id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_gltjsmuqpfeypm6xwdzmj5zty4	Jianhua Zhang	Functional cardiac fibroblasts derived from human pluripotent stem cells via second heart field progenitors	2019.0	15	.pdf	application/pdf	14553	2476	70	differentiated hPSC-CFs exhibit cell morphology, growth, gene expression, fibroblast markers, ECM production, and myofibroblast transformation similar to native human CFs. Results transcription factors ISL1, NKX2-5, and TBX5 indicating commitment of cardiac progenitors in the GiWi protocol (Fig. 1c). These stage-specific progenitors were reproducibly generated from other hPSC lines using the GiWi protocol (Supplementary Fig. 2). fibroblast antibody labeled cells from hPSC-CFs, hfV-CFs, haVCFs, and hDFs populations also uniformly expressed vimentin, d Flow cytometry analysis of cells throughout the GiFGF protocol for expression of the fibroblast marker (anti-human fibroblasts, e Schematic method of the GiFGF protocol and stage-specific progenitors in differentiation of hPSCs to CFs. Gray lines indicate RPMI medium again demonstrated hPSC-CFs and haV-CFs were the most closely related cell populations studied (Fig. 7c). Functional cardiac fibroblasts derived from human pluripotent stem cells via second heart field progenitors Functional cardiac fibroblasts derived from human pluripotent stem cells via second heart field progenitors	./cache/work_gltjsmuqpfeypm6xwdzmj5zty4.pdf	./txt/work_gltjsmuqpfeypm6xwdzmj5zty4.txt