id author title date pages extension mime words sentences flesch summary cache txt work_o4btvfjgwfdtze7ehrivhcvp5i Xin Wang Cellular Fate of Truncated Slow Skeletal Muscle Troponin T Produced by Glu180Nonsense Mutation in Amish Nemaline Myopathy 2005.0 9 .pdf application/pdf 9225 713 65 eukaryotic cells to produce truncated slow TnT protein at high Cloning of Mutant Human Slow TnT cDNA from ANM Patient Muscle—A frozen diagnostic muscle biopsy obtained from the quadriceps poly(A)� mRNA, cDNA encoding slow skeletal muscle TnT was amplified by PCR using oligonucleotide primers synthesized according to the ANM truncated slow TnT to tropomyosin, enzyme-linked immunosorbent assay solid phase protein binding experiments (27) were applied to high and low molecular weight truncated slow TnT proteins, were slow TnT mRNA and truncated protein in skeletal muscle cells. from the transient transfected C2C12 cells confirmed the expression of mutant slow TnT mRNA (data not shown). and lack of truncated slow TnT protein in ANM muscle cells, Cellular Fate of Truncated Slow Skeletal Muscle Troponin T Produced by Glu180 Nonsense Mutation in Amish Nemaline Myopathy* Cellular Fate of Truncated Slow Skeletal Muscle Troponin T Produced by Glu180 Nonsense Mutation in Amish Nemaline Myopathy* ./cache/work_o4btvfjgwfdtze7ehrivhcvp5i.pdf ./txt/work_o4btvfjgwfdtze7ehrivhcvp5i.txt