id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_466tn3xcyjgtbmodeguo2he56i	Orsolya Kolacsek	Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers	2011	8	.pdf	application/pdf	6073	685	47	Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers In this paper, we show that a real-time quantitative PCR-based, transgeneindependent (qPCR-TI) method is able to determine SB transposon copy numbers regardless of the genetic cargo. qPCR-TI method allowed rapid and accurate determination of SB transposon copy numbers in various cell types, Figure 1 Real-time PCR assay designed for different transposon and transgene regions. method could be used for reliable and precise transposon copy-number determination using the IRDR-L TaqMan® assay. To test the qPCR-TI method on other samples, we examined clones of the HUES9 human embryonic stem cell line Figure 2 Comparing copy-number determination by green fluorescent protein (GFP) or transposon-specific real-time PCR. (B) Copy numbers determined by transgene (GFP) specific real-time PCR assay developed transgene-independent method for determining SB transposon copy numbers was validated: (i) it PCR-based (qPCR-TI) method for measuring SB transposon copy numbers. method for determining Sleeping Beauty transposon copy numbers.	./cache/work_466tn3xcyjgtbmodeguo2he56i.pdf	./txt/work_466tn3xcyjgtbmodeguo2he56i.txt