id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_4u5ifj5cdbaqfm53dlrqlbzv6a	Jen-Hsuan Chang	Sleeping Beauty Transposon-Mediated Asparaginase Gene Delivery by a Nanoparticle Platform	2019	9	.pdf	application/pdf	6601	661	62	The Sleeping Beauty (SB) transposon system3 is a non-viral vector that can mediate stable integration of therapeutic transgenes into the genomes of treated cells4,5 and provides sustained expression over a long time. the asparaginase gene as a potent therapy to the asparagine-sensitive cancer cells, especially when co-treated with To investigate the full strength of the asparaginase gene therapy, we performed the asparagine depletion experiments with the ASNase-carried PC9 and ASNase-carried A549 stable cells. PEI-adsorbed MSN showed good uptake and low cytotoxicity to human lung cancer cells. PEI-adsorbed MSN showed good uptake and low cytotoxicity to human lung cancer cells. PEI-absorbed MSN successfully delivered the Sleeping Beauty system for asparaginase expression. Figure 1 The nanoparticle delivery of the Sleeping Beauty transposon system to mediate the asparaginase (ASNase) gene integration into cancer cells. Figure 5 The asparaginase gene was successfully delivered and expressed by the PEI-absorbed MSN transfection in PC9 and A549 cells.	./cache/work_4u5ifj5cdbaqfm53dlrqlbzv6a.pdf	./txt/work_4u5ifj5cdbaqfm53dlrqlbzv6a.txt