id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_aya3cew4hvfrhmf3ur3er2dz4y	Adrian Kovač	RNA-guided retargeting of Sleeping Beauty transposition in human cells	2020	43	.pdf	application/pdf	12256	1081	64	Keywords: genetic engineering, CRISPR/Cas, gene targeting, DNA-binding, gene insertion 23 such as the SB100X transposase catalyze gene transfer in human cells with high efficiency (13). into the target cell's genome with great efficiency and at the same time in a site-specific manner. study failed to target the piggyBac transposon into the HPRT gene with CRISPR/Cas9 132 Next, integration site datasets generated with dCas9-N57 + SB100X + sgAluY-1 (Figure 249 by fusion to dCas9 as an RNA-guided targeting domain in human cells, and as a result be 294 activity of Alu-directed sgRNAs, 1 µg of genomic DNA isolated from human HEK293T cells 446 Ivics, Z., Hackett, P.B., Plasterk, R.H. and Izsvák, Z. Moldt, B., Miskey, C., Staunstrup, N.H., Gogol-Döring, A., Bak, R.O., Sharma, N., Mátés, L., Izsvák, 622 Voigt, K., Gogol-Döring, A., Miskey, C., Chen, W., Cathomen, T., Izsvák, Z. Ammar, I., Gogol-Döring, A., Miskey, C., Chen, W., Cathomen, T., Izsvák, Z.	./cache/work_aya3cew4hvfrhmf3ur3er2dz4y.pdf	./txt/work_aya3cew4hvfrhmf3ur3er2dz4y.txt