id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_cvm5u7naonhzfbhmhlzfmmmgrq	Morgan Newman	A hyperactive sleeping beauty transposase enhances transgenesis in zebrafish embryos	2010	4	.pdf	application/pdf	2406	263	50	zebrafish transgenesis consisting of the inverted repeats of 'sleeping beauty' flanking the gene to be transposed. Using our previously characterised mitfa-amyloidb-GFP transgene, we observed an eight-fold enhancement in transient transgenesis following detection of transgene expression in melanophores Conclusion: The novel hyperactive 'sleeping beauty' transposase enhances the rate of transgenesis in zebrafish Sleeping beauty consists of the transposase gene flanked by terminal inverted repeats of direct we compared the rates of transient transgenesis in zebrafish embryos using the original transposase mRNA mRNA and, once injected into the zebrafish embryos, Zebrafish embryos were injected at the 1-cell stage injected embryos with GFP transcript expression, also had Therefore, it is not possible to observe melanophore-specific GFP expression in these embryos. between transgenesis and embryo defect rates. 'sleeping beauty' transposase enhances the rate of transgenesis in zebrafish embryos. Embryos injected with transgene pT2-mitfa-amyloidb-GFP and SB100x transposase enhances transgenesis in zebrafish embryos.	./cache/work_cvm5u7naonhzfbhmhlzfmmmgrq.pdf	./txt/work_cvm5u7naonhzfbhmhlzfmmmgrq.txt