id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_dfl5dl3uufd7rgphvtgduvnq6a	Darius Balciunas	Enhancer trapping in zebrafish using the Sleeping Beauty transposon	2004	15	.pdf	application/pdf	12255	1115	42	established 9 lines of zebrafish with distinct tissueor organ-specific GFP expression patterns from expresses GFP specifically in caudal primary motoneurons due to an insertion into the poly(ADPribose) glycohydrolase (PARG) locus. Enhancer detection ("trapping") using insertion site context vectors was popularized as a genomics tool in Drosophila. rapid identification of transposon insertion sites responsible for specific GFP expression patterns. enhancer trap lines exhibit GFP expression patterns we did not observe any dependency of the expression pattern on the genomic context of the transposon insertion. Enhancer trap lines exhibit a variety of unique GFP expression patterns. Southern analysis indicated that there is a single transposon insertion in this line, and it is linked to GFP expression (Figure 5). In all lines except ET1 (see above), a single GFP expression-linked transposon insertion event was detected by Many of the obtained enhancer trap lines express GFP in GFP expression in ET2 line embryos is indistinguishable from endogenous PARG gene expressionFigure 6	./cache/work_dfl5dl3uufd7rgphvtgduvnq6a.pdf	./txt/work_dfl5dl3uufd7rgphvtgduvnq6a.txt