id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_kd2rl4y2cvgnfhjpc4tu7iejha	Adam J. Dupuy	Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system	2005	6	.pdf	application/pdf	5023	432	57	member of the Tc1/mariner class of transposons, can be mobilized in mouse somatic cells at frequencies high enough to founder transgenic animals were screened to determine their transposon copy number and the methylation status of the MSCV animals containing a high copy number of unmethylated transposons (6057, 6070, 6113) were used to establish transgenic lines excised from transposon concatomers in double-transgenic embryos, transposons in double-transgenic embryos (Fig. 1e). showed that the normal morphology of the cerebellum was disrupted, with tumour cells invading the molecular layer (Fig. 3a). Analysis of SB integration sites in tumour DNA T2/Onc2 primarily activates gene expression by integrating upstream contained SB integrations in intron 27 of Notch1 (Fig. 4a, Supplementary Table 4). cDNA probe showed that all tumours with Notch1 integrations (lanes 1–4) also had activating integrations upstream of Rasgrp1 (Fig. 5, Supplementary Table 4), a gene that positively regulates Ras signalling.	./cache/work_kd2rl4y2cvgnfhjpc4tu7iejha.pdf	./txt/work_kd2rl4y2cvgnfhjpc4tu7iejha.txt