id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_lg7uwni46nhjxg5pc3yrqf32wy	Donald A Yergeau	Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis	2011	18	.pdf	application/pdf	11871	1029	48	Results: Transgenic frogs that express the SB10 transposase were bred with SB transposon-harboring animals to The remobilized SB transposons frequently integrated near the site of the donor locus; approximately Remobilization of a non-autonomous transposon transgene is achieved by expressing the transposase enzyme in Outcross of the transposase enzyme and transposon substrate double transgenic animals can result in An organism-wide change in GFP intensity (Figure 4a) likely represents modification of the parental transposon donor locus during gametogenesis that Figure 4 Excision and re-integration of SB transposons in the progeny of double-transgenic hopper frogs. observed in individual 8F hopper frogs, with an average rate of two remobilization events per thousand GFP-positive progeny (0.2%). Southern blot analysis of genomic DNA harvested from the progeny of double transgenic 8F hopper frogs. Although the remobilization frequency observed in the outcross of the double transgenic frogs is low, the re-integration events are	./cache/work_lg7uwni46nhjxg5pc3yrqf32wy.pdf	./txt/work_lg7uwni46nhjxg5pc3yrqf32wy.txt