id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_n3bw4ncjifdnfczsjaf4nalnu4	R. Ikeda	Sleeping Beauty Transposase Has an Affinity for Heterochromatin Conformation	2006	12	.pdf	application/pdf	8791	1194	66	The excision frequency of the SB transposon, which is the first step of the transposition event, was enhanced by approximately 100-fold. Using this mechanism, we performed a tTR-based study by inducing heterochromatin formation in inserted tetO sequences within or near the transposon IR/DRs and compared the efficiencies of SB transposition with either the pCAG-IRES-Hygro control or pCAG-tTR-IRES-Hygro expression vector and cultured in ES cell medium containing 200 �g/ml hygromycin B. Genomic DNA was isolated from tTR and control clones after transfection with Transposition of DNA-type transposons generally consists of its excision and subsequent reinsertion into the host These results suggest that tTR effectively induced DNA methylation around the transposon/tetO region. Genomic DNAs from tTR and control clones were digested with methylation-insensitive MspI (shown on the right). Recruitment of SB transposase to tTR-induced heterochromatinized transposons. After the transfection of SB transposase, tTR and control clones were inoculated into 96-well culture plates with the indicated cell numbers.	./cache/work_n3bw4ncjifdnfczsjaf4nalnu4.pdf	./txt/work_n3bw4ncjifdnfczsjaf4nalnu4.txt