id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_sny56posuffadh5a367n5vi4uq	Geyi Liu	Excision ofSleeping Beauty transposons: parameters and applications to gene therapy	2004	10	.pdf	application/pdf	7490	683	64	Excision of Sleeping Beauty transposons: parameters and applications to gene therapy Sleeping Beauty (SB) transposon system as a non-viral vector for gene therapy. that border the sites of excision inside and outside the transposon in order to determine the most active sequences for transposition from a donor plasmid. SB transposons leave characteristically different footprints at excision sites assess transposition in livers of mice that were injected with the SB transposon Sons, Ltd. Keywords footprint; mouse liver; Tc1/mariner; terminal repeats; transposition Copyright  2004 John Wiley & Sons, Ltd. Excision of Sleeping Beauty Transposons 575 steps involved in transposition, the excision of the transposon from the donor site and the integration of the The relative excision activity of the mutated transposon To sequence the footprint, the PCR products were gelextracted (for mutations with low excision activity, Excision sites were sequenced using primers outside the transposon. PCR analysis of transposon excision from plasmids in	./cache/work_sny56posuffadh5a367n5vi4uq.pdf	./txt/work_sny56posuffadh5a367n5vi4uq.txt