id sid tid token lemma pos work_infoczas7nfbzac5mhjnxpkxle 1 1 General general JJ work_infoczas7nfbzac5mhjnxpkxle 1 2 rights right NNS work_infoczas7nfbzac5mhjnxpkxle 1 3 Copyright copyright NN work_infoczas7nfbzac5mhjnxpkxle 1 4 and and CC work_infoczas7nfbzac5mhjnxpkxle 1 5 moral moral JJ work_infoczas7nfbzac5mhjnxpkxle 1 6 rights right NNS work_infoczas7nfbzac5mhjnxpkxle 1 7 for for IN work_infoczas7nfbzac5mhjnxpkxle 1 8 the the DT work_infoczas7nfbzac5mhjnxpkxle 1 9 publications publication NNS work_infoczas7nfbzac5mhjnxpkxle 1 10 made make VBN work_infoczas7nfbzac5mhjnxpkxle 1 11 accessible accessible JJ work_infoczas7nfbzac5mhjnxpkxle 1 12 in in IN work_infoczas7nfbzac5mhjnxpkxle 1 13 the the DT work_infoczas7nfbzac5mhjnxpkxle 1 14 public public JJ work_infoczas7nfbzac5mhjnxpkxle 1 15 portal portal NN work_infoczas7nfbzac5mhjnxpkxle 1 16 are be VBP work_infoczas7nfbzac5mhjnxpkxle 1 17 retained retain VBN work_infoczas7nfbzac5mhjnxpkxle 1 18 by by IN work_infoczas7nfbzac5mhjnxpkxle 1 19 the the DT work_infoczas7nfbzac5mhjnxpkxle 1 20 authors author NNS work_infoczas7nfbzac5mhjnxpkxle 1 21 and/or and/or CC work_infoczas7nfbzac5mhjnxpkxle 1 22 other other JJ work_infoczas7nfbzac5mhjnxpkxle 1 23 copyright copyright NN work_infoczas7nfbzac5mhjnxpkxle 1 24 owners owner NNS work_infoczas7nfbzac5mhjnxpkxle 1 25 and and CC work_infoczas7nfbzac5mhjnxpkxle 1 26 it -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 1 27 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 1 28 a a DT work_infoczas7nfbzac5mhjnxpkxle 1 29 condition condition NN work_infoczas7nfbzac5mhjnxpkxle 1 30 of of IN work_infoczas7nfbzac5mhjnxpkxle 1 31 accessing access VBG work_infoczas7nfbzac5mhjnxpkxle 1 32 publications publication NNS work_infoczas7nfbzac5mhjnxpkxle 1 33 that that WDT work_infoczas7nfbzac5mhjnxpkxle 1 34 users user NNS work_infoczas7nfbzac5mhjnxpkxle 1 35 recognise recognise VBP work_infoczas7nfbzac5mhjnxpkxle 1 36 and and CC work_infoczas7nfbzac5mhjnxpkxle 1 37 abide abide VBP work_infoczas7nfbzac5mhjnxpkxle 1 38 by by IN work_infoczas7nfbzac5mhjnxpkxle 1 39 the the DT work_infoczas7nfbzac5mhjnxpkxle 1 40 legal legal JJ work_infoczas7nfbzac5mhjnxpkxle 1 41 requirements requirement NNS work_infoczas7nfbzac5mhjnxpkxle 1 42 associated associate VBN work_infoczas7nfbzac5mhjnxpkxle 1 43 with with IN work_infoczas7nfbzac5mhjnxpkxle 1 44 these these DT work_infoczas7nfbzac5mhjnxpkxle 1 45 rights right NNS work_infoczas7nfbzac5mhjnxpkxle 1 46 . . . work_infoczas7nfbzac5mhjnxpkxle 2 1   NFP work_infoczas7nfbzac5mhjnxpkxle 2 2 Users user NNS work_infoczas7nfbzac5mhjnxpkxle 2 3 may may MD work_infoczas7nfbzac5mhjnxpkxle 2 4 download download VB work_infoczas7nfbzac5mhjnxpkxle 2 5 and and CC work_infoczas7nfbzac5mhjnxpkxle 2 6 print print VB work_infoczas7nfbzac5mhjnxpkxle 2 7 one one CD work_infoczas7nfbzac5mhjnxpkxle 2 8 copy copy NN work_infoczas7nfbzac5mhjnxpkxle 2 9 of of IN work_infoczas7nfbzac5mhjnxpkxle 2 10 any any DT work_infoczas7nfbzac5mhjnxpkxle 2 11 publication publication NN work_infoczas7nfbzac5mhjnxpkxle 2 12 from from IN work_infoczas7nfbzac5mhjnxpkxle 2 13 the the DT work_infoczas7nfbzac5mhjnxpkxle 2 14 public public JJ work_infoczas7nfbzac5mhjnxpkxle 2 15 portal portal NN work_infoczas7nfbzac5mhjnxpkxle 2 16 for for IN work_infoczas7nfbzac5mhjnxpkxle 2 17 the the DT work_infoczas7nfbzac5mhjnxpkxle 2 18 purpose purpose NN work_infoczas7nfbzac5mhjnxpkxle 2 19 of of IN work_infoczas7nfbzac5mhjnxpkxle 2 20 private private JJ work_infoczas7nfbzac5mhjnxpkxle 2 21 study study NN work_infoczas7nfbzac5mhjnxpkxle 2 22 or or CC work_infoczas7nfbzac5mhjnxpkxle 2 23 research research NN work_infoczas7nfbzac5mhjnxpkxle 2 24 . . . work_infoczas7nfbzac5mhjnxpkxle 3 1   NFP work_infoczas7nfbzac5mhjnxpkxle 3 2 You -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 3 3 may may MD work_infoczas7nfbzac5mhjnxpkxle 3 4 not not RB work_infoczas7nfbzac5mhjnxpkxle 3 5 further further RB work_infoczas7nfbzac5mhjnxpkxle 3 6 distribute distribute VB work_infoczas7nfbzac5mhjnxpkxle 3 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 3 8 material material NN work_infoczas7nfbzac5mhjnxpkxle 3 9 or or CC work_infoczas7nfbzac5mhjnxpkxle 3 10 use use VB work_infoczas7nfbzac5mhjnxpkxle 3 11 it -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 3 12 for for IN work_infoczas7nfbzac5mhjnxpkxle 3 13 any any DT work_infoczas7nfbzac5mhjnxpkxle 3 14 profit profit NN work_infoczas7nfbzac5mhjnxpkxle 3 15 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 3 16 making make VBG work_infoczas7nfbzac5mhjnxpkxle 3 17 activity activity NN work_infoczas7nfbzac5mhjnxpkxle 3 18 or or CC work_infoczas7nfbzac5mhjnxpkxle 3 19 commercial commercial JJ work_infoczas7nfbzac5mhjnxpkxle 3 20 gain gain NN work_infoczas7nfbzac5mhjnxpkxle 3 21   : work_infoczas7nfbzac5mhjnxpkxle 3 22 You -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 3 23 may may MD work_infoczas7nfbzac5mhjnxpkxle 3 24 freely freely RB work_infoczas7nfbzac5mhjnxpkxle 3 25 distribute distribute VB work_infoczas7nfbzac5mhjnxpkxle 3 26 the the DT work_infoczas7nfbzac5mhjnxpkxle 3 27 URL url NN work_infoczas7nfbzac5mhjnxpkxle 3 28 identifying identify VBG work_infoczas7nfbzac5mhjnxpkxle 3 29 the the DT work_infoczas7nfbzac5mhjnxpkxle 3 30 publication publication NN work_infoczas7nfbzac5mhjnxpkxle 3 31 in in IN work_infoczas7nfbzac5mhjnxpkxle 3 32 the the DT work_infoczas7nfbzac5mhjnxpkxle 3 33 public public JJ work_infoczas7nfbzac5mhjnxpkxle 3 34 portal portal NN work_infoczas7nfbzac5mhjnxpkxle 3 35 If if IN work_infoczas7nfbzac5mhjnxpkxle 3 36 you -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 3 37 believe believe VBP work_infoczas7nfbzac5mhjnxpkxle 3 38 that that IN work_infoczas7nfbzac5mhjnxpkxle 3 39 this this DT work_infoczas7nfbzac5mhjnxpkxle 3 40 document document NN work_infoczas7nfbzac5mhjnxpkxle 3 41 breaches breach VBZ work_infoczas7nfbzac5mhjnxpkxle 3 42 copyright copyright NN work_infoczas7nfbzac5mhjnxpkxle 3 43 please please UH work_infoczas7nfbzac5mhjnxpkxle 3 44 contact contact VB work_infoczas7nfbzac5mhjnxpkxle 3 45 us -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 3 46 providing provide VBG work_infoczas7nfbzac5mhjnxpkxle 3 47 details detail NNS work_infoczas7nfbzac5mhjnxpkxle 3 48 , , , work_infoczas7nfbzac5mhjnxpkxle 3 49 and and CC work_infoczas7nfbzac5mhjnxpkxle 3 50 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 3 51 will will MD work_infoczas7nfbzac5mhjnxpkxle 3 52 remove remove VB work_infoczas7nfbzac5mhjnxpkxle 3 53 access access NN work_infoczas7nfbzac5mhjnxpkxle 3 54 to to IN work_infoczas7nfbzac5mhjnxpkxle 3 55 the the DT work_infoczas7nfbzac5mhjnxpkxle 3 56 work work NN work_infoczas7nfbzac5mhjnxpkxle 3 57 immediately immediately RB work_infoczas7nfbzac5mhjnxpkxle 3 58 and and CC work_infoczas7nfbzac5mhjnxpkxle 3 59 investigate investigate VB work_infoczas7nfbzac5mhjnxpkxle 3 60 your -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 3 61 claim claim NN work_infoczas7nfbzac5mhjnxpkxle 3 62 . . . work_infoczas7nfbzac5mhjnxpkxle 4 1 Downloaded download VBN work_infoczas7nfbzac5mhjnxpkxle 4 2 from from IN work_infoczas7nfbzac5mhjnxpkxle 4 3 orbit.dtu.dk orbit.dtu.dk NNP work_infoczas7nfbzac5mhjnxpkxle 4 4 on on IN work_infoczas7nfbzac5mhjnxpkxle 4 5 : : : work_infoczas7nfbzac5mhjnxpkxle 4 6 Apr Apr NNP work_infoczas7nfbzac5mhjnxpkxle 4 7 06 06 CD work_infoczas7nfbzac5mhjnxpkxle 4 8 , , , work_infoczas7nfbzac5mhjnxpkxle 4 9 2021 2021 CD work_infoczas7nfbzac5mhjnxpkxle 4 10 Pig pig NN work_infoczas7nfbzac5mhjnxpkxle 4 11 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 4 12 by by IN work_infoczas7nfbzac5mhjnxpkxle 4 13 Sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 4 14 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 4 15 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 4 16 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 4 17 Jakobsen Jakobsen NNP work_infoczas7nfbzac5mhjnxpkxle 4 18 , , , work_infoczas7nfbzac5mhjnxpkxle 4 19 Jannik Jannik NNP work_infoczas7nfbzac5mhjnxpkxle 4 20 E. E. NNP work_infoczas7nfbzac5mhjnxpkxle 4 21 ; ; : work_infoczas7nfbzac5mhjnxpkxle 4 22 Li Li NNP work_infoczas7nfbzac5mhjnxpkxle 4 23 , , , work_infoczas7nfbzac5mhjnxpkxle 4 24 Juan Juan NNP work_infoczas7nfbzac5mhjnxpkxle 4 25 ; ; : work_infoczas7nfbzac5mhjnxpkxle 4 26 Kragh Kragh NNP work_infoczas7nfbzac5mhjnxpkxle 4 27 , , , work_infoczas7nfbzac5mhjnxpkxle 4 28 Peter Peter NNP work_infoczas7nfbzac5mhjnxpkxle 4 29 M. M. NNP work_infoczas7nfbzac5mhjnxpkxle 4 30 ; ; : work_infoczas7nfbzac5mhjnxpkxle 4 31 Moldt Moldt NNP work_infoczas7nfbzac5mhjnxpkxle 4 32 , , , work_infoczas7nfbzac5mhjnxpkxle 4 33 Brian Brian NNP work_infoczas7nfbzac5mhjnxpkxle 4 34 ; ; : work_infoczas7nfbzac5mhjnxpkxle 4 35 Lin Lin NNP work_infoczas7nfbzac5mhjnxpkxle 4 36 , , , work_infoczas7nfbzac5mhjnxpkxle 4 37 Lin Lin NNP work_infoczas7nfbzac5mhjnxpkxle 4 38 ; ; : work_infoczas7nfbzac5mhjnxpkxle 4 39 Liu Liu NNP work_infoczas7nfbzac5mhjnxpkxle 4 40 , , , work_infoczas7nfbzac5mhjnxpkxle 4 41 Ying Ying NNP work_infoczas7nfbzac5mhjnxpkxle 4 42 ; ; : work_infoczas7nfbzac5mhjnxpkxle 4 43 Schmidt Schmidt NNP work_infoczas7nfbzac5mhjnxpkxle 4 44 , , , work_infoczas7nfbzac5mhjnxpkxle 4 45 Mette Mette NNP work_infoczas7nfbzac5mhjnxpkxle 4 46 ; ; : work_infoczas7nfbzac5mhjnxpkxle 4 47 Winther Winther NNP work_infoczas7nfbzac5mhjnxpkxle 4 48 , , , work_infoczas7nfbzac5mhjnxpkxle 4 49 Kjeld Kjeld NNP work_infoczas7nfbzac5mhjnxpkxle 4 50 D. D. NNP work_infoczas7nfbzac5mhjnxpkxle 4 51 ; ; : work_infoczas7nfbzac5mhjnxpkxle 4 52 Schyth Schyth NNP work_infoczas7nfbzac5mhjnxpkxle 4 53 , , , work_infoczas7nfbzac5mhjnxpkxle 4 54 Brian Brian NNP work_infoczas7nfbzac5mhjnxpkxle 4 55 Dall Dall NNP work_infoczas7nfbzac5mhjnxpkxle 4 56 ; ; : work_infoczas7nfbzac5mhjnxpkxle 4 57 Holm Holm NNP work_infoczas7nfbzac5mhjnxpkxle 4 58 , , , work_infoczas7nfbzac5mhjnxpkxle 4 59 Ida Ida NNP work_infoczas7nfbzac5mhjnxpkxle 4 60 E. E. NNP work_infoczas7nfbzac5mhjnxpkxle 4 61 Total Total NNP work_infoczas7nfbzac5mhjnxpkxle 4 62 number number NN work_infoczas7nfbzac5mhjnxpkxle 4 63 of of IN work_infoczas7nfbzac5mhjnxpkxle 4 64 authors author NNS work_infoczas7nfbzac5mhjnxpkxle 4 65 : : : work_infoczas7nfbzac5mhjnxpkxle 4 66 16 16 CD work_infoczas7nfbzac5mhjnxpkxle 4 67 Published publish VBN work_infoczas7nfbzac5mhjnxpkxle 4 68 in in IN work_infoczas7nfbzac5mhjnxpkxle 4 69 : : : work_infoczas7nfbzac5mhjnxpkxle 4 70 Transgenic Transgenic NNP work_infoczas7nfbzac5mhjnxpkxle 4 71 Research Research NNP work_infoczas7nfbzac5mhjnxpkxle 4 72 Link Link NNP work_infoczas7nfbzac5mhjnxpkxle 4 73 to to IN work_infoczas7nfbzac5mhjnxpkxle 4 74 article article NN work_infoczas7nfbzac5mhjnxpkxle 4 75 , , , work_infoczas7nfbzac5mhjnxpkxle 4 76 DOI DOI NNP work_infoczas7nfbzac5mhjnxpkxle 4 77 : : : work_infoczas7nfbzac5mhjnxpkxle 4 78 10.1007 10.1007 CD work_infoczas7nfbzac5mhjnxpkxle 4 79 / / SYM work_infoczas7nfbzac5mhjnxpkxle 4 80 s11248 s11248 CD work_infoczas7nfbzac5mhjnxpkxle 4 81 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 4 82 010 010 CD work_infoczas7nfbzac5mhjnxpkxle 4 83 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 4 84 9438-x 9438-x NNP work_infoczas7nfbzac5mhjnxpkxle 4 85 Publication Publication NNP work_infoczas7nfbzac5mhjnxpkxle 4 86 date date NN work_infoczas7nfbzac5mhjnxpkxle 4 87 : : : work_infoczas7nfbzac5mhjnxpkxle 4 88 2011 2011 CD work_infoczas7nfbzac5mhjnxpkxle 4 89 Link link VB work_infoczas7nfbzac5mhjnxpkxle 4 90 back back RB work_infoczas7nfbzac5mhjnxpkxle 4 91 to to IN work_infoczas7nfbzac5mhjnxpkxle 4 92 DTU DTU NNP work_infoczas7nfbzac5mhjnxpkxle 4 93 Orbit Orbit NNP work_infoczas7nfbzac5mhjnxpkxle 4 94 Citation Citation NNP work_infoczas7nfbzac5mhjnxpkxle 4 95 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 4 96 APA APA NNP work_infoczas7nfbzac5mhjnxpkxle 4 97 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 4 98 : : : work_infoczas7nfbzac5mhjnxpkxle 4 99 Jakobsen Jakobsen NNP work_infoczas7nfbzac5mhjnxpkxle 4 100 , , , work_infoczas7nfbzac5mhjnxpkxle 4 101 J. J. NNP work_infoczas7nfbzac5mhjnxpkxle 4 102 E. E. NNP work_infoczas7nfbzac5mhjnxpkxle 4 103 , , , work_infoczas7nfbzac5mhjnxpkxle 4 104 Li Li NNP work_infoczas7nfbzac5mhjnxpkxle 4 105 , , , work_infoczas7nfbzac5mhjnxpkxle 4 106 J. J. NNP work_infoczas7nfbzac5mhjnxpkxle 4 107 , , , work_infoczas7nfbzac5mhjnxpkxle 4 108 Kragh Kragh NNP work_infoczas7nfbzac5mhjnxpkxle 4 109 , , , work_infoczas7nfbzac5mhjnxpkxle 4 110 P. P. NNP work_infoczas7nfbzac5mhjnxpkxle 4 111 M. M. NNP work_infoczas7nfbzac5mhjnxpkxle 4 112 , , , work_infoczas7nfbzac5mhjnxpkxle 4 113 Moldt Moldt NNP work_infoczas7nfbzac5mhjnxpkxle 4 114 , , , work_infoczas7nfbzac5mhjnxpkxle 4 115 B. B. NNP work_infoczas7nfbzac5mhjnxpkxle 4 116 , , , work_infoczas7nfbzac5mhjnxpkxle 4 117 Lin Lin NNP work_infoczas7nfbzac5mhjnxpkxle 4 118 , , , work_infoczas7nfbzac5mhjnxpkxle 4 119 L. L. NNP work_infoczas7nfbzac5mhjnxpkxle 4 120 , , , work_infoczas7nfbzac5mhjnxpkxle 4 121 Liu Liu NNP work_infoczas7nfbzac5mhjnxpkxle 4 122 , , , work_infoczas7nfbzac5mhjnxpkxle 4 123 Y. Y. NNP work_infoczas7nfbzac5mhjnxpkxle 4 124 , , , work_infoczas7nfbzac5mhjnxpkxle 4 125 Schmidt Schmidt NNP work_infoczas7nfbzac5mhjnxpkxle 4 126 , , , work_infoczas7nfbzac5mhjnxpkxle 4 127 M. M. NNP work_infoczas7nfbzac5mhjnxpkxle 4 128 , , , work_infoczas7nfbzac5mhjnxpkxle 4 129 Winther Winther NNP work_infoczas7nfbzac5mhjnxpkxle 4 130 , , , work_infoczas7nfbzac5mhjnxpkxle 4 131 K. K. NNP work_infoczas7nfbzac5mhjnxpkxle 4 132 D. D. NNP work_infoczas7nfbzac5mhjnxpkxle 4 133 , , , work_infoczas7nfbzac5mhjnxpkxle 4 134 Schyth Schyth NNP work_infoczas7nfbzac5mhjnxpkxle 4 135 , , , work_infoczas7nfbzac5mhjnxpkxle 4 136 B. B. NNP work_infoczas7nfbzac5mhjnxpkxle 4 137 D. D. NNP work_infoczas7nfbzac5mhjnxpkxle 4 138 , , , work_infoczas7nfbzac5mhjnxpkxle 4 139 Holm Holm NNP work_infoczas7nfbzac5mhjnxpkxle 4 140 , , , work_infoczas7nfbzac5mhjnxpkxle 4 141 I. I. NNP work_infoczas7nfbzac5mhjnxpkxle 4 142 E. E. NNP work_infoczas7nfbzac5mhjnxpkxle 4 143 , , , work_infoczas7nfbzac5mhjnxpkxle 4 144 Vajta Vajta NNP work_infoczas7nfbzac5mhjnxpkxle 4 145 , , , work_infoczas7nfbzac5mhjnxpkxle 4 146 G. G. NNP work_infoczas7nfbzac5mhjnxpkxle 4 147 , , , work_infoczas7nfbzac5mhjnxpkxle 4 148 Bolund Bolund NNP work_infoczas7nfbzac5mhjnxpkxle 4 149 , , , work_infoczas7nfbzac5mhjnxpkxle 4 150 L. L. NNP work_infoczas7nfbzac5mhjnxpkxle 4 151 , , , work_infoczas7nfbzac5mhjnxpkxle 4 152 Callesen Callesen NNP work_infoczas7nfbzac5mhjnxpkxle 4 153 , , , work_infoczas7nfbzac5mhjnxpkxle 4 154 H. H. NNP work_infoczas7nfbzac5mhjnxpkxle 4 155 , , , work_infoczas7nfbzac5mhjnxpkxle 4 156 Jørgensen Jørgensen NNP work_infoczas7nfbzac5mhjnxpkxle 4 157 , , , work_infoczas7nfbzac5mhjnxpkxle 4 158 A. A. NNP work_infoczas7nfbzac5mhjnxpkxle 4 159 L. L. NNP work_infoczas7nfbzac5mhjnxpkxle 4 160 , , , work_infoczas7nfbzac5mhjnxpkxle 4 161 Nielsen Nielsen NNP work_infoczas7nfbzac5mhjnxpkxle 4 162 , , , work_infoczas7nfbzac5mhjnxpkxle 4 163 A. A. NNP work_infoczas7nfbzac5mhjnxpkxle 4 164 L. L. NNP work_infoczas7nfbzac5mhjnxpkxle 4 165 , , , work_infoczas7nfbzac5mhjnxpkxle 4 166 & & CC work_infoczas7nfbzac5mhjnxpkxle 4 167 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 4 168 , , , work_infoczas7nfbzac5mhjnxpkxle 4 169 J. J. NNP work_infoczas7nfbzac5mhjnxpkxle 4 170 G. G. NNP work_infoczas7nfbzac5mhjnxpkxle 4 171 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 4 172 2011 2011 CD work_infoczas7nfbzac5mhjnxpkxle 4 173 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 4 174 . . . work_infoczas7nfbzac5mhjnxpkxle 5 1 Pig pig NN work_infoczas7nfbzac5mhjnxpkxle 5 2 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 5 3 by by IN work_infoczas7nfbzac5mhjnxpkxle 5 4 Sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 5 5 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 5 6 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 5 7 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 5 8 . . . work_infoczas7nfbzac5mhjnxpkxle 6 1 Transgenic Transgenic NNP work_infoczas7nfbzac5mhjnxpkxle 6 2 Research Research NNP work_infoczas7nfbzac5mhjnxpkxle 6 3 , , , work_infoczas7nfbzac5mhjnxpkxle 6 4 20(3 20(3 CD work_infoczas7nfbzac5mhjnxpkxle 6 5 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 6 6 , , , work_infoczas7nfbzac5mhjnxpkxle 6 7 533 533 CD work_infoczas7nfbzac5mhjnxpkxle 6 8 - - SYM work_infoczas7nfbzac5mhjnxpkxle 6 9 545 545 CD work_infoczas7nfbzac5mhjnxpkxle 6 10 . . . work_infoczas7nfbzac5mhjnxpkxle 6 11 https://doi.org/10.1007/s11248-010-9438-x https://doi.org/10.1007/s11248-010-9438-x NNP work_infoczas7nfbzac5mhjnxpkxle 6 12 https://doi.org/10.1007/s11248-010-9438-x https://doi.org/10.1007/s11248-010-9438-x NNP work_infoczas7nfbzac5mhjnxpkxle 6 13 https://orbit.dtu.dk/en/publications/55dbe82e-989b-4eeb-b6e6-ccba9197f594 https://orbit.dtu.dk/en/publications/55dbe82e-989b-4eeb-b6e6-ccba9197f594 NNP work_infoczas7nfbzac5mhjnxpkxle 6 14 https://doi.org/10.1007/s11248-010-9438-x https://doi.org/10.1007/s11248-010-9438-x NNP work_infoczas7nfbzac5mhjnxpkxle 6 15 Editorial Editorial NNP work_infoczas7nfbzac5mhjnxpkxle 6 16 Manager(tm Manager(tm NNS work_infoczas7nfbzac5mhjnxpkxle 6 17 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 6 18 for for IN work_infoczas7nfbzac5mhjnxpkxle 6 19 Transgenic Transgenic NNP work_infoczas7nfbzac5mhjnxpkxle 6 20 Research Research NNP work_infoczas7nfbzac5mhjnxpkxle 6 21 Manuscript Manuscript NNP work_infoczas7nfbzac5mhjnxpkxle 6 22 Draft Draft NNP work_infoczas7nfbzac5mhjnxpkxle 6 23 Manuscript Manuscript NNP work_infoczas7nfbzac5mhjnxpkxle 6 24 Number Number NNP work_infoczas7nfbzac5mhjnxpkxle 6 25 : : : work_infoczas7nfbzac5mhjnxpkxle 6 26 TRAG1116R3 trag1116r3 NN work_infoczas7nfbzac5mhjnxpkxle 6 27 Title title NN work_infoczas7nfbzac5mhjnxpkxle 6 28 : : : work_infoczas7nfbzac5mhjnxpkxle 6 29 Pig pig NN work_infoczas7nfbzac5mhjnxpkxle 6 30 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 6 31 by by IN work_infoczas7nfbzac5mhjnxpkxle 6 32 Sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 6 33 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 6 34 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 6 35 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 6 36 Article article NN work_infoczas7nfbzac5mhjnxpkxle 6 37 Type type NN work_infoczas7nfbzac5mhjnxpkxle 6 38 : : : work_infoczas7nfbzac5mhjnxpkxle 6 39 Original original JJ work_infoczas7nfbzac5mhjnxpkxle 6 40 research research NN work_infoczas7nfbzac5mhjnxpkxle 6 41 papers paper NNS work_infoczas7nfbzac5mhjnxpkxle 6 42 Section Section NNP work_infoczas7nfbzac5mhjnxpkxle 6 43 / / SYM work_infoczas7nfbzac5mhjnxpkxle 6 44 Category category NN work_infoczas7nfbzac5mhjnxpkxle 6 45 : : : work_infoczas7nfbzac5mhjnxpkxle 6 46 Animal Animal NNP work_infoczas7nfbzac5mhjnxpkxle 6 47 Section Section NNP work_infoczas7nfbzac5mhjnxpkxle 6 48 Keywords Keywords NNPS work_infoczas7nfbzac5mhjnxpkxle 6 49 : : : work_infoczas7nfbzac5mhjnxpkxle 6 50 Pig pig NN work_infoczas7nfbzac5mhjnxpkxle 6 51 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 6 52 Somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 6 53 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 6 54 nuclear nuclear JJ work_infoczas7nfbzac5mhjnxpkxle 6 55 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 6 56 Genetic genetic JJ work_infoczas7nfbzac5mhjnxpkxle 6 57 engineering engineering NN work_infoczas7nfbzac5mhjnxpkxle 6 58 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 6 59 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 6 60 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 6 61 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 6 62 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 6 63 transposase transposase VBP work_infoczas7nfbzac5mhjnxpkxle 6 64 Systemic systemic JJ work_infoczas7nfbzac5mhjnxpkxle 6 65 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 6 66 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 6 67 Corresponding Corresponding NNP work_infoczas7nfbzac5mhjnxpkxle 6 68 Author author NN work_infoczas7nfbzac5mhjnxpkxle 6 69 : : : work_infoczas7nfbzac5mhjnxpkxle 6 70 Dr. Dr. NNP work_infoczas7nfbzac5mhjnxpkxle 6 71 Jacob Jacob NNP work_infoczas7nfbzac5mhjnxpkxle 6 72 Giehm Giehm NNP work_infoczas7nfbzac5mhjnxpkxle 6 73 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 6 74 , , , work_infoczas7nfbzac5mhjnxpkxle 6 75 Ph.D. ph.d. NN work_infoczas7nfbzac5mhjnxpkxle 7 1 Corresponding Corresponding NNP work_infoczas7nfbzac5mhjnxpkxle 7 2 Author author NN work_infoczas7nfbzac5mhjnxpkxle 7 3 's 's POS work_infoczas7nfbzac5mhjnxpkxle 7 4 Institution Institution NNP work_infoczas7nfbzac5mhjnxpkxle 7 5 : : : work_infoczas7nfbzac5mhjnxpkxle 7 6 University University NNP work_infoczas7nfbzac5mhjnxpkxle 7 7 of of IN work_infoczas7nfbzac5mhjnxpkxle 7 8 Aarhus Aarhus NNP work_infoczas7nfbzac5mhjnxpkxle 7 9 First First NNP work_infoczas7nfbzac5mhjnxpkxle 7 10 Author author NN work_infoczas7nfbzac5mhjnxpkxle 7 11 : : : work_infoczas7nfbzac5mhjnxpkxle 7 12 Jannik Jannik NNP work_infoczas7nfbzac5mhjnxpkxle 7 13 E E NNP work_infoczas7nfbzac5mhjnxpkxle 7 14 Jakobsen Jakobsen NNP work_infoczas7nfbzac5mhjnxpkxle 7 15 Order Order NNP work_infoczas7nfbzac5mhjnxpkxle 7 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 7 17 Authors author NNS work_infoczas7nfbzac5mhjnxpkxle 7 18 : : : work_infoczas7nfbzac5mhjnxpkxle 7 19 Jannik Jannik NNP work_infoczas7nfbzac5mhjnxpkxle 7 20 E E NNP work_infoczas7nfbzac5mhjnxpkxle 7 21 Jakobsen Jakobsen NNP work_infoczas7nfbzac5mhjnxpkxle 7 22 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 23 Juan Juan NNP work_infoczas7nfbzac5mhjnxpkxle 7 24 Li Li NNP work_infoczas7nfbzac5mhjnxpkxle 7 25 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 26 Peter Peter NNP work_infoczas7nfbzac5mhjnxpkxle 7 27 M M NNP work_infoczas7nfbzac5mhjnxpkxle 7 28 Kragh Kragh NNP work_infoczas7nfbzac5mhjnxpkxle 7 29 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 30 Brian Brian NNP work_infoczas7nfbzac5mhjnxpkxle 7 31 Moldt Moldt NNP work_infoczas7nfbzac5mhjnxpkxle 7 32 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 33 Lin Lin NNP work_infoczas7nfbzac5mhjnxpkxle 7 34 Lin Lin NNP work_infoczas7nfbzac5mhjnxpkxle 7 35 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 36 Ying Ying NNP work_infoczas7nfbzac5mhjnxpkxle 7 37 Liu Liu NNP work_infoczas7nfbzac5mhjnxpkxle 7 38 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 39 Mette Mette NNP work_infoczas7nfbzac5mhjnxpkxle 7 40 Schmidt Schmidt NNP work_infoczas7nfbzac5mhjnxpkxle 7 41 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 42 Kjeld Kjeld NNP work_infoczas7nfbzac5mhjnxpkxle 7 43 D D NNP work_infoczas7nfbzac5mhjnxpkxle 7 44 Winther Winther NNP work_infoczas7nfbzac5mhjnxpkxle 7 45 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 46 Brian Brian NNP work_infoczas7nfbzac5mhjnxpkxle 7 47 D D NNP work_infoczas7nfbzac5mhjnxpkxle 7 48 Schyth Schyth NNP work_infoczas7nfbzac5mhjnxpkxle 7 49 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 50 Ida Ida NNP work_infoczas7nfbzac5mhjnxpkxle 7 51 E E NNP work_infoczas7nfbzac5mhjnxpkxle 7 52 Holm Holm NNP work_infoczas7nfbzac5mhjnxpkxle 7 53 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 54 Gabor Gabor NNP work_infoczas7nfbzac5mhjnxpkxle 7 55 Vajta Vajta NNP work_infoczas7nfbzac5mhjnxpkxle 7 56 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 57 Lars Lars NNP work_infoczas7nfbzac5mhjnxpkxle 7 58 Bolund Bolund NNP work_infoczas7nfbzac5mhjnxpkxle 7 59 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 60 Henrik Henrik NNP work_infoczas7nfbzac5mhjnxpkxle 7 61 Callesen Callesen NNP work_infoczas7nfbzac5mhjnxpkxle 7 62 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 63 Arne Arne NNP work_infoczas7nfbzac5mhjnxpkxle 7 64 L L NNP work_infoczas7nfbzac5mhjnxpkxle 7 65 Jørgensen Jørgensen NNP work_infoczas7nfbzac5mhjnxpkxle 7 66 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 67 Anders Anders NNP work_infoczas7nfbzac5mhjnxpkxle 7 68 L L NNP work_infoczas7nfbzac5mhjnxpkxle 7 69 Nielsen Nielsen NNP work_infoczas7nfbzac5mhjnxpkxle 7 70 ; ; : work_infoczas7nfbzac5mhjnxpkxle 7 71 Jacob Jacob NNP work_infoczas7nfbzac5mhjnxpkxle 7 72 G G NNP work_infoczas7nfbzac5mhjnxpkxle 7 73 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 7 74 Abstract Abstract NNP work_infoczas7nfbzac5mhjnxpkxle 7 75 : : : work_infoczas7nfbzac5mhjnxpkxle 7 76 Modelling model VBG work_infoczas7nfbzac5mhjnxpkxle 7 77 of of IN work_infoczas7nfbzac5mhjnxpkxle 7 78 human human JJ work_infoczas7nfbzac5mhjnxpkxle 7 79 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 7 80 in in IN work_infoczas7nfbzac5mhjnxpkxle 7 81 genetically genetically RB work_infoczas7nfbzac5mhjnxpkxle 7 82 engineered engineer VBN work_infoczas7nfbzac5mhjnxpkxle 7 83 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 7 84 provides provide VBZ work_infoczas7nfbzac5mhjnxpkxle 7 85 unique unique JJ work_infoczas7nfbzac5mhjnxpkxle 7 86 possibilities possibility NNS work_infoczas7nfbzac5mhjnxpkxle 7 87 in in IN work_infoczas7nfbzac5mhjnxpkxle 7 88 biomedical biomedical JJ work_infoczas7nfbzac5mhjnxpkxle 7 89 research research NN work_infoczas7nfbzac5mhjnxpkxle 7 90 and and CC work_infoczas7nfbzac5mhjnxpkxle 7 91 in in IN work_infoczas7nfbzac5mhjnxpkxle 7 92 studies study NNS work_infoczas7nfbzac5mhjnxpkxle 7 93 of of IN work_infoczas7nfbzac5mhjnxpkxle 7 94 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 7 95 intervention intervention NN work_infoczas7nfbzac5mhjnxpkxle 7 96 . . . work_infoczas7nfbzac5mhjnxpkxle 8 1 Establishment establishment NN work_infoczas7nfbzac5mhjnxpkxle 8 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 8 3 methodologies methodology NNS work_infoczas7nfbzac5mhjnxpkxle 8 4 that that WDT work_infoczas7nfbzac5mhjnxpkxle 8 5 allow allow VBP work_infoczas7nfbzac5mhjnxpkxle 8 6 efficient efficient JJ work_infoczas7nfbzac5mhjnxpkxle 8 7 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 8 8 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 8 9 by by IN work_infoczas7nfbzac5mhjnxpkxle 8 10 non non JJ work_infoczas7nfbzac5mhjnxpkxle 8 11 - - JJ work_infoczas7nfbzac5mhjnxpkxle 8 12 viral viral JJ work_infoczas7nfbzac5mhjnxpkxle 8 13 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 8 14 carriers carrier NNS work_infoczas7nfbzac5mhjnxpkxle 8 15 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 8 16 an an DT work_infoczas7nfbzac5mhjnxpkxle 8 17 important important JJ work_infoczas7nfbzac5mhjnxpkxle 8 18 step step NN work_infoczas7nfbzac5mhjnxpkxle 8 19 towards towards IN work_infoczas7nfbzac5mhjnxpkxle 8 20 development development NN work_infoczas7nfbzac5mhjnxpkxle 8 21 of of IN work_infoczas7nfbzac5mhjnxpkxle 8 22 new new JJ work_infoczas7nfbzac5mhjnxpkxle 8 23 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 8 24 models model NNS work_infoczas7nfbzac5mhjnxpkxle 8 25 . . . work_infoczas7nfbzac5mhjnxpkxle 9 1 In in IN work_infoczas7nfbzac5mhjnxpkxle 9 2 this this DT work_infoczas7nfbzac5mhjnxpkxle 9 3 report report NN work_infoczas7nfbzac5mhjnxpkxle 9 4 , , , work_infoczas7nfbzac5mhjnxpkxle 9 5 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 9 6 present present VBP work_infoczas7nfbzac5mhjnxpkxle 9 7 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 9 8 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 9 9 created create VBN work_infoczas7nfbzac5mhjnxpkxle 9 10 by by IN work_infoczas7nfbzac5mhjnxpkxle 9 11 Sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 9 12 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 9 13 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 9 14 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 9 15 in in IN work_infoczas7nfbzac5mhjnxpkxle 9 16 primary primary JJ work_infoczas7nfbzac5mhjnxpkxle 9 17 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 9 18 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 9 19 in in IN work_infoczas7nfbzac5mhjnxpkxle 9 20 combination combination NN work_infoczas7nfbzac5mhjnxpkxle 9 21 with with IN work_infoczas7nfbzac5mhjnxpkxle 9 22 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 9 23 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 9 24 nuclear nuclear JJ work_infoczas7nfbzac5mhjnxpkxle 9 25 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 9 26 by by IN work_infoczas7nfbzac5mhjnxpkxle 9 27 handmade handmade NN work_infoczas7nfbzac5mhjnxpkxle 9 28 cloning cloning NN work_infoczas7nfbzac5mhjnxpkxle 9 29 . . . work_infoczas7nfbzac5mhjnxpkxle 10 1 Göttingen Göttingen NNP work_infoczas7nfbzac5mhjnxpkxle 10 2 minipigs minipig NNS work_infoczas7nfbzac5mhjnxpkxle 10 3 expressing express VBG work_infoczas7nfbzac5mhjnxpkxle 10 4 green green JJ work_infoczas7nfbzac5mhjnxpkxle 10 5 fluorescent fluorescent NN work_infoczas7nfbzac5mhjnxpkxle 10 6 protein protein NN work_infoczas7nfbzac5mhjnxpkxle 10 7 are be VBP work_infoczas7nfbzac5mhjnxpkxle 10 8 produced produce VBN work_infoczas7nfbzac5mhjnxpkxle 10 9 by by IN work_infoczas7nfbzac5mhjnxpkxle 10 10 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 10 11 with with IN work_infoczas7nfbzac5mhjnxpkxle 10 12 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 10 13 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 10 14 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 10 15 carrying carry VBG work_infoczas7nfbzac5mhjnxpkxle 10 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 10 17 transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 10 18 driven drive VBN work_infoczas7nfbzac5mhjnxpkxle 10 19 by by IN work_infoczas7nfbzac5mhjnxpkxle 10 20 the the DT work_infoczas7nfbzac5mhjnxpkxle 10 21 human human NNP work_infoczas7nfbzac5mhjnxpkxle 10 22 ubiquitin ubiquitin NNP work_infoczas7nfbzac5mhjnxpkxle 10 23 C C NNP work_infoczas7nfbzac5mhjnxpkxle 10 24 promoter promoter NN work_infoczas7nfbzac5mhjnxpkxle 10 25 . . . work_infoczas7nfbzac5mhjnxpkxle 11 1 These these DT work_infoczas7nfbzac5mhjnxpkxle 11 2 animals animal NNS work_infoczas7nfbzac5mhjnxpkxle 11 3 carry carry VBP work_infoczas7nfbzac5mhjnxpkxle 11 4 multiple multiple JJ work_infoczas7nfbzac5mhjnxpkxle 11 5 copies copy NNS work_infoczas7nfbzac5mhjnxpkxle 11 6 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 11 7 from from IN work_infoczas7nfbzac5mhjnxpkxle 11 8 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 11 9 to to IN work_infoczas7nfbzac5mhjnxpkxle 11 10 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 11 11 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 11 12 of of IN work_infoczas7nfbzac5mhjnxpkxle 11 13 the the DT work_infoczas7nfbzac5mhjnxpkxle 11 14 transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 11 15 and and CC work_infoczas7nfbzac5mhjnxpkxle 11 16 show show VB work_infoczas7nfbzac5mhjnxpkxle 11 17 systemic systemic JJ work_infoczas7nfbzac5mhjnxpkxle 11 18 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 11 19 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 11 20 . . . work_infoczas7nfbzac5mhjnxpkxle 12 1 Transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 12 2 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 12 3 expressing express VBG work_infoczas7nfbzac5mhjnxpkxle 12 4 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 12 5 carry carry VBP work_infoczas7nfbzac5mhjnxpkxle 12 6 both both DT work_infoczas7nfbzac5mhjnxpkxle 12 7 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 12 8 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 12 9 catalyzed catalyzed JJ work_infoczas7nfbzac5mhjnxpkxle 12 10 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 12 11 and and CC work_infoczas7nfbzac5mhjnxpkxle 12 12 at at RB work_infoczas7nfbzac5mhjnxpkxle 12 13 least least RBS work_infoczas7nfbzac5mhjnxpkxle 12 14 one one CD work_infoczas7nfbzac5mhjnxpkxle 12 15 copy copy NN work_infoczas7nfbzac5mhjnxpkxle 12 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 12 17 randomly randomly RB work_infoczas7nfbzac5mhjnxpkxle 12 18 inserted insert VBN work_infoczas7nfbzac5mhjnxpkxle 12 19 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 12 20 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 12 21 . . . work_infoczas7nfbzac5mhjnxpkxle 13 1 Our -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 13 2 findings finding NNS work_infoczas7nfbzac5mhjnxpkxle 13 3 illustrate illustrate VBP work_infoczas7nfbzac5mhjnxpkxle 13 4 critical critical JJ work_infoczas7nfbzac5mhjnxpkxle 13 5 issues issue NNS work_infoczas7nfbzac5mhjnxpkxle 13 6 related relate VBN work_infoczas7nfbzac5mhjnxpkxle 13 7 to to IN work_infoczas7nfbzac5mhjnxpkxle 13 8 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 13 9 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 13 10 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 13 11 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 13 12 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 13 13 , , , work_infoczas7nfbzac5mhjnxpkxle 13 14 including include VBG work_infoczas7nfbzac5mhjnxpkxle 13 15 coincidental coincidental NNP work_infoczas7nfbzac5mhjnxpkxle 13 16 plasmid plasmid NNP work_infoczas7nfbzac5mhjnxpkxle 13 17 insertion insertion NNP work_infoczas7nfbzac5mhjnxpkxle 13 18 and and CC work_infoczas7nfbzac5mhjnxpkxle 13 19 relatively relatively RB work_infoczas7nfbzac5mhjnxpkxle 13 20 low low JJ work_infoczas7nfbzac5mhjnxpkxle 13 21 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 13 22 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 13 23 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 13 24 activity activity NN work_infoczas7nfbzac5mhjnxpkxle 13 25 in in IN work_infoczas7nfbzac5mhjnxpkxle 13 26 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 13 27 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 13 28 , , , work_infoczas7nfbzac5mhjnxpkxle 13 29 but but CC work_infoczas7nfbzac5mhjnxpkxle 13 30 also also RB work_infoczas7nfbzac5mhjnxpkxle 13 31 provide provide VB work_infoczas7nfbzac5mhjnxpkxle 13 32 a a DT work_infoczas7nfbzac5mhjnxpkxle 13 33 platform platform NN work_infoczas7nfbzac5mhjnxpkxle 13 34 for for IN work_infoczas7nfbzac5mhjnxpkxle 13 35 future future JJ work_infoczas7nfbzac5mhjnxpkxle 13 36 development development NN work_infoczas7nfbzac5mhjnxpkxle 13 37 of of IN work_infoczas7nfbzac5mhjnxpkxle 13 38 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 13 39 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 13 40 models model NNS work_infoczas7nfbzac5mhjnxpkxle 13 41 using use VBG work_infoczas7nfbzac5mhjnxpkxle 13 42 the the DT work_infoczas7nfbzac5mhjnxpkxle 13 43 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 13 44 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 13 45 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 13 46 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 13 47 technology technology NN work_infoczas7nfbzac5mhjnxpkxle 13 48 . . . work_infoczas7nfbzac5mhjnxpkxle 14 1 1 1 LS work_infoczas7nfbzac5mhjnxpkxle 14 2 TRAG1116_R3 trag1116_r3 NN work_infoczas7nfbzac5mhjnxpkxle 14 3 Pig Pig NNP work_infoczas7nfbzac5mhjnxpkxle 14 4 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 14 5 by by IN work_infoczas7nfbzac5mhjnxpkxle 14 6 Sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 14 7 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 14 8 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 14 9 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 14 10 Jannik Jannik NNP work_infoczas7nfbzac5mhjnxpkxle 14 11 E. E. NNP work_infoczas7nfbzac5mhjnxpkxle 14 12 Jakobsen Jakobsen NNP work_infoczas7nfbzac5mhjnxpkxle 14 13 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 14 14 , , , work_infoczas7nfbzac5mhjnxpkxle 14 15 Juan Juan NNP work_infoczas7nfbzac5mhjnxpkxle 14 16 Li Li NNP work_infoczas7nfbzac5mhjnxpkxle 14 17 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 14 18 , , , work_infoczas7nfbzac5mhjnxpkxle 14 19 Peter Peter NNP work_infoczas7nfbzac5mhjnxpkxle 14 20 M. M. NNP work_infoczas7nfbzac5mhjnxpkxle 14 21 Kragh Kragh NNP work_infoczas7nfbzac5mhjnxpkxle 14 22 1,2 1,2 CD work_infoczas7nfbzac5mhjnxpkxle 14 23 , , , work_infoczas7nfbzac5mhjnxpkxle 14 24 Brian Brian NNP work_infoczas7nfbzac5mhjnxpkxle 14 25 Moldt Moldt NNP work_infoczas7nfbzac5mhjnxpkxle 14 26 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 14 27 , , , work_infoczas7nfbzac5mhjnxpkxle 14 28 Lin Lin NNP work_infoczas7nfbzac5mhjnxpkxle 14 29 Lin Lin NNP work_infoczas7nfbzac5mhjnxpkxle 14 30 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 14 31 , , , work_infoczas7nfbzac5mhjnxpkxle 14 32 Ying Ying NNP work_infoczas7nfbzac5mhjnxpkxle 14 33 Liu Liu NNP work_infoczas7nfbzac5mhjnxpkxle 14 34 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 14 35 , , , work_infoczas7nfbzac5mhjnxpkxle 14 36 Mette Mette NNP work_infoczas7nfbzac5mhjnxpkxle 14 37 Schmidt Schmidt NNP work_infoczas7nfbzac5mhjnxpkxle 14 38 3 3 CD work_infoczas7nfbzac5mhjnxpkxle 14 39 , , , work_infoczas7nfbzac5mhjnxpkxle 14 40 Kjeld Kjeld NNP work_infoczas7nfbzac5mhjnxpkxle 14 41 Dahl Dahl NNP work_infoczas7nfbzac5mhjnxpkxle 14 42 Winther Winther NNP work_infoczas7nfbzac5mhjnxpkxle 14 43 4 4 CD work_infoczas7nfbzac5mhjnxpkxle 14 44 , , , work_infoczas7nfbzac5mhjnxpkxle 14 45 Brian Brian NNP work_infoczas7nfbzac5mhjnxpkxle 14 46 Dall Dall NNP work_infoczas7nfbzac5mhjnxpkxle 14 47 Schyth Schyth NNP work_infoczas7nfbzac5mhjnxpkxle 14 48 5 5 CD work_infoczas7nfbzac5mhjnxpkxle 14 49 , , , work_infoczas7nfbzac5mhjnxpkxle 14 50 Ida Ida NNP work_infoczas7nfbzac5mhjnxpkxle 14 51 E. E. NNP work_infoczas7nfbzac5mhjnxpkxle 14 52 Holm Holm NNP work_infoczas7nfbzac5mhjnxpkxle 14 53 6,7 6,7 CD work_infoczas7nfbzac5mhjnxpkxle 14 54 , , , work_infoczas7nfbzac5mhjnxpkxle 14 55 Gábor Gábor NNP work_infoczas7nfbzac5mhjnxpkxle 14 56 Vajta Vajta NNP work_infoczas7nfbzac5mhjnxpkxle 14 57 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 14 58 , , , work_infoczas7nfbzac5mhjnxpkxle 14 59 Lars Lars NNP work_infoczas7nfbzac5mhjnxpkxle 14 60 Bolund Bolund NNP work_infoczas7nfbzac5mhjnxpkxle 14 61 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 14 62 , , , work_infoczas7nfbzac5mhjnxpkxle 14 63 Henrik Henrik NNP work_infoczas7nfbzac5mhjnxpkxle 14 64 Callesen Callesen NNP work_infoczas7nfbzac5mhjnxpkxle 14 65 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 14 66 , , , work_infoczas7nfbzac5mhjnxpkxle 14 67 Arne Arne NNP work_infoczas7nfbzac5mhjnxpkxle 14 68 Lund Lund NNP work_infoczas7nfbzac5mhjnxpkxle 14 69 Jørgensen Jørgensen NNP work_infoczas7nfbzac5mhjnxpkxle 14 70 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 14 71 , , , work_infoczas7nfbzac5mhjnxpkxle 14 72 Anders Anders NNPS work_infoczas7nfbzac5mhjnxpkxle 14 73 Lade Lade NNP work_infoczas7nfbzac5mhjnxpkxle 14 74 Nielsen Nielsen NNP work_infoczas7nfbzac5mhjnxpkxle 14 75 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 14 76 * * NFP work_infoczas7nfbzac5mhjnxpkxle 14 77 , , , work_infoczas7nfbzac5mhjnxpkxle 14 78 and and CC work_infoczas7nfbzac5mhjnxpkxle 14 79 Jacob Jacob NNP work_infoczas7nfbzac5mhjnxpkxle 14 80 Giehm Giehm NNP work_infoczas7nfbzac5mhjnxpkxle 14 81 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 14 82 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 14 83 * * NFP work_infoczas7nfbzac5mhjnxpkxle 14 84 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 14 85 Department Department NNP work_infoczas7nfbzac5mhjnxpkxle 14 86 of of IN work_infoczas7nfbzac5mhjnxpkxle 14 87 Human Human NNP work_infoczas7nfbzac5mhjnxpkxle 14 88 Genetics Genetics NNPS work_infoczas7nfbzac5mhjnxpkxle 14 89 , , , work_infoczas7nfbzac5mhjnxpkxle 14 90 University University NNP work_infoczas7nfbzac5mhjnxpkxle 14 91 of of IN work_infoczas7nfbzac5mhjnxpkxle 14 92 Aarhus Aarhus NNP work_infoczas7nfbzac5mhjnxpkxle 14 93 , , , work_infoczas7nfbzac5mhjnxpkxle 14 94 DK-8000 DK-8000 NNP work_infoczas7nfbzac5mhjnxpkxle 14 95 Aarhus Aarhus NNP work_infoczas7nfbzac5mhjnxpkxle 14 96 C C NNP work_infoczas7nfbzac5mhjnxpkxle 14 97 , , , work_infoczas7nfbzac5mhjnxpkxle 14 98 Denmark Denmark NNP work_infoczas7nfbzac5mhjnxpkxle 14 99 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 14 100 Department Department NNP work_infoczas7nfbzac5mhjnxpkxle 14 101 of of IN work_infoczas7nfbzac5mhjnxpkxle 14 102 Genetics Genetics NNPS work_infoczas7nfbzac5mhjnxpkxle 14 103 and and CC work_infoczas7nfbzac5mhjnxpkxle 14 104 Biotechnology Biotechnology NNP work_infoczas7nfbzac5mhjnxpkxle 14 105 , , , work_infoczas7nfbzac5mhjnxpkxle 14 106 Faculty Faculty NNP work_infoczas7nfbzac5mhjnxpkxle 14 107 of of IN work_infoczas7nfbzac5mhjnxpkxle 14 108 Agricultural Agricultural NNP work_infoczas7nfbzac5mhjnxpkxle 14 109 Sciences Sciences NNPS work_infoczas7nfbzac5mhjnxpkxle 14 110 , , , work_infoczas7nfbzac5mhjnxpkxle 14 111 University University NNP work_infoczas7nfbzac5mhjnxpkxle 14 112 of of IN work_infoczas7nfbzac5mhjnxpkxle 14 113 Aarhus Aarhus NNP work_infoczas7nfbzac5mhjnxpkxle 14 114 , , , work_infoczas7nfbzac5mhjnxpkxle 14 115 DK- DK- NNP work_infoczas7nfbzac5mhjnxpkxle 14 116 8830 8830 CD work_infoczas7nfbzac5mhjnxpkxle 14 117 Tjele Tjele NNP work_infoczas7nfbzac5mhjnxpkxle 14 118 , , , work_infoczas7nfbzac5mhjnxpkxle 14 119 Denmark Denmark NNP work_infoczas7nfbzac5mhjnxpkxle 14 120 3 3 CD work_infoczas7nfbzac5mhjnxpkxle 14 121 Department Department NNP work_infoczas7nfbzac5mhjnxpkxle 14 122 of of IN work_infoczas7nfbzac5mhjnxpkxle 14 123 Veterinary Veterinary NNP work_infoczas7nfbzac5mhjnxpkxle 14 124 Reproduction Reproduction NNP work_infoczas7nfbzac5mhjnxpkxle 14 125 and and CC work_infoczas7nfbzac5mhjnxpkxle 14 126 Obstetrics Obstetrics NNP work_infoczas7nfbzac5mhjnxpkxle 14 127 , , , work_infoczas7nfbzac5mhjnxpkxle 14 128 Faculty Faculty NNP work_infoczas7nfbzac5mhjnxpkxle 14 129 of of IN work_infoczas7nfbzac5mhjnxpkxle 14 130 Life Life NNP work_infoczas7nfbzac5mhjnxpkxle 14 131 Sciences Sciences NNPS work_infoczas7nfbzac5mhjnxpkxle 14 132 , , , work_infoczas7nfbzac5mhjnxpkxle 14 133 University University NNP work_infoczas7nfbzac5mhjnxpkxle 14 134 of of IN work_infoczas7nfbzac5mhjnxpkxle 14 135 Copenhagen Copenhagen NNP work_infoczas7nfbzac5mhjnxpkxle 14 136 , , , work_infoczas7nfbzac5mhjnxpkxle 14 137 DK-1870 DK-1870 NNP work_infoczas7nfbzac5mhjnxpkxle 14 138 Frederiksberg Frederiksberg NNP work_infoczas7nfbzac5mhjnxpkxle 14 139 C C NNP work_infoczas7nfbzac5mhjnxpkxle 14 140 , , , work_infoczas7nfbzac5mhjnxpkxle 14 141 Denmark Denmark NNP work_infoczas7nfbzac5mhjnxpkxle 14 142 4 4 CD work_infoczas7nfbzac5mhjnxpkxle 14 143 Danish Danish NNP work_infoczas7nfbzac5mhjnxpkxle 14 144 Agriculture Agriculture NNP work_infoczas7nfbzac5mhjnxpkxle 14 145 and and CC work_infoczas7nfbzac5mhjnxpkxle 14 146 Food Food NNP work_infoczas7nfbzac5mhjnxpkxle 14 147 Council Council NNP work_infoczas7nfbzac5mhjnxpkxle 14 148 , , , work_infoczas7nfbzac5mhjnxpkxle 14 149 Knowledge Knowledge NNP work_infoczas7nfbzac5mhjnxpkxle 14 150 Center Center NNP work_infoczas7nfbzac5mhjnxpkxle 14 151 on on IN work_infoczas7nfbzac5mhjnxpkxle 14 152 Pig Pig NNP work_infoczas7nfbzac5mhjnxpkxle 14 153 production production NN work_infoczas7nfbzac5mhjnxpkxle 14 154 , , , work_infoczas7nfbzac5mhjnxpkxle 14 155 DK-1609 DK-1609 NNP work_infoczas7nfbzac5mhjnxpkxle 14 156 Copenhagen Copenhagen NNP work_infoczas7nfbzac5mhjnxpkxle 14 157 V V NNP work_infoczas7nfbzac5mhjnxpkxle 14 158 , , , work_infoczas7nfbzac5mhjnxpkxle 14 159 Denmark Denmark NNP work_infoczas7nfbzac5mhjnxpkxle 14 160 5 5 CD work_infoczas7nfbzac5mhjnxpkxle 14 161 National National NNP work_infoczas7nfbzac5mhjnxpkxle 14 162 Veterinary Veterinary NNP work_infoczas7nfbzac5mhjnxpkxle 14 163 Institute Institute NNP work_infoczas7nfbzac5mhjnxpkxle 14 164 , , , work_infoczas7nfbzac5mhjnxpkxle 14 165 Technical Technical NNP work_infoczas7nfbzac5mhjnxpkxle 14 166 University University NNP work_infoczas7nfbzac5mhjnxpkxle 14 167 of of IN work_infoczas7nfbzac5mhjnxpkxle 14 168 Denmark Denmark NNP work_infoczas7nfbzac5mhjnxpkxle 14 169 , , , work_infoczas7nfbzac5mhjnxpkxle 14 170 DK-8200 DK-8200 NNP work_infoczas7nfbzac5mhjnxpkxle 14 171 Aarhus Aarhus NNP work_infoczas7nfbzac5mhjnxpkxle 14 172 N N NNP work_infoczas7nfbzac5mhjnxpkxle 14 173 , , , work_infoczas7nfbzac5mhjnxpkxle 14 174 Denmark Denmark NNP work_infoczas7nfbzac5mhjnxpkxle 14 175 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 14 176 Department Department NNP work_infoczas7nfbzac5mhjnxpkxle 14 177 of of IN work_infoczas7nfbzac5mhjnxpkxle 14 178 Pathology Pathology NNP work_infoczas7nfbzac5mhjnxpkxle 14 179 , , , work_infoczas7nfbzac5mhjnxpkxle 14 180 Randers Randers NNPS work_infoczas7nfbzac5mhjnxpkxle 14 181 Hospital Hospital NNP work_infoczas7nfbzac5mhjnxpkxle 14 182 , , , work_infoczas7nfbzac5mhjnxpkxle 14 183 DK-8900 DK-8900 NNP work_infoczas7nfbzac5mhjnxpkxle 14 184 Randers Randers NNP work_infoczas7nfbzac5mhjnxpkxle 14 185 , , , work_infoczas7nfbzac5mhjnxpkxle 14 186 Denmark Denmark NNP work_infoczas7nfbzac5mhjnxpkxle 14 187 7 7 CD work_infoczas7nfbzac5mhjnxpkxle 14 188 Danish Danish NNP work_infoczas7nfbzac5mhjnxpkxle 14 189 Neuroscience Neuroscience NNP work_infoczas7nfbzac5mhjnxpkxle 14 190 Center Center NNP work_infoczas7nfbzac5mhjnxpkxle 14 191 , , , work_infoczas7nfbzac5mhjnxpkxle 14 192 Aarhus Aarhus NNP work_infoczas7nfbzac5mhjnxpkxle 14 193 University University NNP work_infoczas7nfbzac5mhjnxpkxle 14 194 Hospital Hospital NNP work_infoczas7nfbzac5mhjnxpkxle 14 195 , , , work_infoczas7nfbzac5mhjnxpkxle 14 196 DK-8000 DK-8000 NNP work_infoczas7nfbzac5mhjnxpkxle 14 197 Aarhus Aarhus NNP work_infoczas7nfbzac5mhjnxpkxle 14 198 C C NNP work_infoczas7nfbzac5mhjnxpkxle 14 199 * * NFP work_infoczas7nfbzac5mhjnxpkxle 14 200 These these DT work_infoczas7nfbzac5mhjnxpkxle 14 201 authors author NNS work_infoczas7nfbzac5mhjnxpkxle 14 202 contributed contribute VBD work_infoczas7nfbzac5mhjnxpkxle 14 203 equally equally RB work_infoczas7nfbzac5mhjnxpkxle 14 204 to to IN work_infoczas7nfbzac5mhjnxpkxle 14 205 this this DT work_infoczas7nfbzac5mhjnxpkxle 14 206 work work NN work_infoczas7nfbzac5mhjnxpkxle 14 207 Running run VBG work_infoczas7nfbzac5mhjnxpkxle 14 208 title title NN work_infoczas7nfbzac5mhjnxpkxle 14 209 : : : work_infoczas7nfbzac5mhjnxpkxle 14 210 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 14 211 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 14 212 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 14 213 mediated mediate VBN work_infoczas7nfbzac5mhjnxpkxle 14 214 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 14 215 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 14 216 Word Word NNP work_infoczas7nfbzac5mhjnxpkxle 14 217 count count NN work_infoczas7nfbzac5mhjnxpkxle 14 218 abstract abstract NN work_infoczas7nfbzac5mhjnxpkxle 14 219 : : : work_infoczas7nfbzac5mhjnxpkxle 14 220 169 169 CD work_infoczas7nfbzac5mhjnxpkxle 14 221 Word Word NNP work_infoczas7nfbzac5mhjnxpkxle 14 222 count count NN work_infoczas7nfbzac5mhjnxpkxle 14 223 text text NN work_infoczas7nfbzac5mhjnxpkxle 14 224 : : : work_infoczas7nfbzac5mhjnxpkxle 14 225 4728 4728 CD work_infoczas7nfbzac5mhjnxpkxle 14 226 * * NFP work_infoczas7nfbzac5mhjnxpkxle 14 227 Corresponding Corresponding NNP work_infoczas7nfbzac5mhjnxpkxle 14 228 author author NN work_infoczas7nfbzac5mhjnxpkxle 14 229 Jacob Jacob NNP work_infoczas7nfbzac5mhjnxpkxle 14 230 Giehm Giehm NNP work_infoczas7nfbzac5mhjnxpkxle 14 231 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 14 232 , , , work_infoczas7nfbzac5mhjnxpkxle 14 233 Department Department NNP work_infoczas7nfbzac5mhjnxpkxle 14 234 of of IN work_infoczas7nfbzac5mhjnxpkxle 14 235 Human Human NNP work_infoczas7nfbzac5mhjnxpkxle 14 236 Genetics Genetics NNP work_infoczas7nfbzac5mhjnxpkxle 14 237 , , , work_infoczas7nfbzac5mhjnxpkxle 14 238 University University NNP work_infoczas7nfbzac5mhjnxpkxle 14 239 of of IN work_infoczas7nfbzac5mhjnxpkxle 14 240 Aarhus Aarhus NNP work_infoczas7nfbzac5mhjnxpkxle 14 241 , , , work_infoczas7nfbzac5mhjnxpkxle 14 242 Wilh Wilh NNP work_infoczas7nfbzac5mhjnxpkxle 14 243 . . . work_infoczas7nfbzac5mhjnxpkxle 15 1 Meyers Meyers NNP work_infoczas7nfbzac5mhjnxpkxle 15 2 Allé Allé NNP work_infoczas7nfbzac5mhjnxpkxle 15 3 1240 1240 CD work_infoczas7nfbzac5mhjnxpkxle 15 4 , , , work_infoczas7nfbzac5mhjnxpkxle 15 5 DK-8000 DK-8000 NNP work_infoczas7nfbzac5mhjnxpkxle 15 6 Aarhus Aarhus NNP work_infoczas7nfbzac5mhjnxpkxle 15 7 C C NNP work_infoczas7nfbzac5mhjnxpkxle 15 8 , , , work_infoczas7nfbzac5mhjnxpkxle 15 9 Denmark Denmark NNP work_infoczas7nfbzac5mhjnxpkxle 15 10 . . . work_infoczas7nfbzac5mhjnxpkxle 16 1 Phone phone NN work_infoczas7nfbzac5mhjnxpkxle 16 2 : : : work_infoczas7nfbzac5mhjnxpkxle 16 3 +45 +45 NN work_infoczas7nfbzac5mhjnxpkxle 16 4 89421651 89421651 CD work_infoczas7nfbzac5mhjnxpkxle 16 5 . . . work_infoczas7nfbzac5mhjnxpkxle 17 1 Email email NN work_infoczas7nfbzac5mhjnxpkxle 17 2 : : : work_infoczas7nfbzac5mhjnxpkxle 17 3 giehm@humgen.au.dk giehm@humgen.au.dk NNP work_infoczas7nfbzac5mhjnxpkxle 17 4 Manuscript Manuscript NNP work_infoczas7nfbzac5mhjnxpkxle 17 5 Click Click NNP work_infoczas7nfbzac5mhjnxpkxle 17 6 here here RB work_infoczas7nfbzac5mhjnxpkxle 17 7 to to TO work_infoczas7nfbzac5mhjnxpkxle 17 8 view view VB work_infoczas7nfbzac5mhjnxpkxle 17 9 linked link VBN work_infoczas7nfbzac5mhjnxpkxle 17 10 References reference NNS work_infoczas7nfbzac5mhjnxpkxle 17 11 mailto:giehm@humgen.au.dk mailto:giehm@humgen.au.dk NNP work_infoczas7nfbzac5mhjnxpkxle 17 12 http://www.editorialmanager.com/trag/viewRCResults.aspx?pdf=1&docID=1548&rev=3&fileID=25121&msid={B201DFBC-BBE3-42FE-95A7-A056ACDBCE87 http://www.editorialmanager.com/trag/viewRCResults.aspx?pdf=1&docID=1548&rev=3&fileID=25121&msid={B201DFBC-BBE3-42FE-95A7-A056ACDBCE87 NNP work_infoczas7nfbzac5mhjnxpkxle 17 13 } } -RRB- work_infoczas7nfbzac5mhjnxpkxle 17 14 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 17 15 ABSTRACT ABSTRACT NNP work_infoczas7nfbzac5mhjnxpkxle 17 16 Modelling modelling NN work_infoczas7nfbzac5mhjnxpkxle 17 17 of of IN work_infoczas7nfbzac5mhjnxpkxle 17 18 human human JJ work_infoczas7nfbzac5mhjnxpkxle 17 19 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 17 20 in in IN work_infoczas7nfbzac5mhjnxpkxle 17 21 genetically genetically RB work_infoczas7nfbzac5mhjnxpkxle 17 22 engineered engineer VBN work_infoczas7nfbzac5mhjnxpkxle 17 23 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 17 24 provides provide VBZ work_infoczas7nfbzac5mhjnxpkxle 17 25 unique unique JJ work_infoczas7nfbzac5mhjnxpkxle 17 26 possibilities possibility NNS work_infoczas7nfbzac5mhjnxpkxle 17 27 in in IN work_infoczas7nfbzac5mhjnxpkxle 17 28 biomedical biomedical JJ work_infoczas7nfbzac5mhjnxpkxle 17 29 research research NN work_infoczas7nfbzac5mhjnxpkxle 17 30 and and CC work_infoczas7nfbzac5mhjnxpkxle 17 31 in in IN work_infoczas7nfbzac5mhjnxpkxle 17 32 studies study NNS work_infoczas7nfbzac5mhjnxpkxle 17 33 of of IN work_infoczas7nfbzac5mhjnxpkxle 17 34 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 17 35 intervention intervention NN work_infoczas7nfbzac5mhjnxpkxle 17 36 . . . work_infoczas7nfbzac5mhjnxpkxle 18 1 Establishment establishment NN work_infoczas7nfbzac5mhjnxpkxle 18 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 18 3 methodologies methodology NNS work_infoczas7nfbzac5mhjnxpkxle 18 4 that that WDT work_infoczas7nfbzac5mhjnxpkxle 18 5 allow allow VBP work_infoczas7nfbzac5mhjnxpkxle 18 6 efficient efficient JJ work_infoczas7nfbzac5mhjnxpkxle 18 7 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 18 8 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 18 9 by by IN work_infoczas7nfbzac5mhjnxpkxle 18 10 non non JJ work_infoczas7nfbzac5mhjnxpkxle 18 11 - - JJ work_infoczas7nfbzac5mhjnxpkxle 18 12 viral viral JJ work_infoczas7nfbzac5mhjnxpkxle 18 13 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 18 14 carriers carrier NNS work_infoczas7nfbzac5mhjnxpkxle 18 15 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 18 16 an an DT work_infoczas7nfbzac5mhjnxpkxle 18 17 important important JJ work_infoczas7nfbzac5mhjnxpkxle 18 18 step step NN work_infoczas7nfbzac5mhjnxpkxle 18 19 towards towards IN work_infoczas7nfbzac5mhjnxpkxle 18 20 development development NN work_infoczas7nfbzac5mhjnxpkxle 18 21 of of IN work_infoczas7nfbzac5mhjnxpkxle 18 22 new new JJ work_infoczas7nfbzac5mhjnxpkxle 18 23 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 18 24 models model NNS work_infoczas7nfbzac5mhjnxpkxle 18 25 . . . work_infoczas7nfbzac5mhjnxpkxle 19 1 In in IN work_infoczas7nfbzac5mhjnxpkxle 19 2 this this DT work_infoczas7nfbzac5mhjnxpkxle 19 3 report report NN work_infoczas7nfbzac5mhjnxpkxle 19 4 , , , work_infoczas7nfbzac5mhjnxpkxle 19 5 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 19 6 present present VBP work_infoczas7nfbzac5mhjnxpkxle 19 7 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 19 8 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 19 9 created create VBN work_infoczas7nfbzac5mhjnxpkxle 19 10 by by IN work_infoczas7nfbzac5mhjnxpkxle 19 11 Sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 19 12 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 19 13 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 19 14 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 19 15 in in IN work_infoczas7nfbzac5mhjnxpkxle 19 16 primary primary JJ work_infoczas7nfbzac5mhjnxpkxle 19 17 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 19 18 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 19 19 in in IN work_infoczas7nfbzac5mhjnxpkxle 19 20 combination combination NN work_infoczas7nfbzac5mhjnxpkxle 19 21 with with IN work_infoczas7nfbzac5mhjnxpkxle 19 22 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 19 23 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 19 24 nuclear nuclear JJ work_infoczas7nfbzac5mhjnxpkxle 19 25 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 19 26 by by IN work_infoczas7nfbzac5mhjnxpkxle 19 27 handmade handmade NN work_infoczas7nfbzac5mhjnxpkxle 19 28 cloning cloning NN work_infoczas7nfbzac5mhjnxpkxle 19 29 . . . work_infoczas7nfbzac5mhjnxpkxle 20 1 Göttingen Göttingen NNP work_infoczas7nfbzac5mhjnxpkxle 20 2 minipigs minipig NNS work_infoczas7nfbzac5mhjnxpkxle 20 3 expressing express VBG work_infoczas7nfbzac5mhjnxpkxle 20 4 green green JJ work_infoczas7nfbzac5mhjnxpkxle 20 5 fluorescent fluorescent NN work_infoczas7nfbzac5mhjnxpkxle 20 6 protein protein NN work_infoczas7nfbzac5mhjnxpkxle 20 7 are be VBP work_infoczas7nfbzac5mhjnxpkxle 20 8 produced produce VBN work_infoczas7nfbzac5mhjnxpkxle 20 9 by by IN work_infoczas7nfbzac5mhjnxpkxle 20 10 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 20 11 with with IN work_infoczas7nfbzac5mhjnxpkxle 20 12 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 20 13 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 20 14 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 20 15 carrying carry VBG work_infoczas7nfbzac5mhjnxpkxle 20 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 20 17 transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 20 18 driven drive VBN work_infoczas7nfbzac5mhjnxpkxle 20 19 by by IN work_infoczas7nfbzac5mhjnxpkxle 20 20 the the DT work_infoczas7nfbzac5mhjnxpkxle 20 21 human human NNP work_infoczas7nfbzac5mhjnxpkxle 20 22 ubiquitin ubiquitin NNP work_infoczas7nfbzac5mhjnxpkxle 20 23 C C NNP work_infoczas7nfbzac5mhjnxpkxle 20 24 promoter promoter NN work_infoczas7nfbzac5mhjnxpkxle 20 25 . . . work_infoczas7nfbzac5mhjnxpkxle 21 1 These these DT work_infoczas7nfbzac5mhjnxpkxle 21 2 animals animal NNS work_infoczas7nfbzac5mhjnxpkxle 21 3 carry carry VBP work_infoczas7nfbzac5mhjnxpkxle 21 4 multiple multiple JJ work_infoczas7nfbzac5mhjnxpkxle 21 5 copies copy NNS work_infoczas7nfbzac5mhjnxpkxle 21 6 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 21 7 from from IN work_infoczas7nfbzac5mhjnxpkxle 21 8 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 21 9 to to IN work_infoczas7nfbzac5mhjnxpkxle 21 10 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 21 11 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 21 12 of of IN work_infoczas7nfbzac5mhjnxpkxle 21 13 the the DT work_infoczas7nfbzac5mhjnxpkxle 21 14 transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 21 15 and and CC work_infoczas7nfbzac5mhjnxpkxle 21 16 show show VB work_infoczas7nfbzac5mhjnxpkxle 21 17 systemic systemic JJ work_infoczas7nfbzac5mhjnxpkxle 21 18 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 21 19 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 21 20 . . . work_infoczas7nfbzac5mhjnxpkxle 22 1 Transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 22 2 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 22 3 expressing express VBG work_infoczas7nfbzac5mhjnxpkxle 22 4 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 22 5 carry carry VBP work_infoczas7nfbzac5mhjnxpkxle 22 6 both both DT work_infoczas7nfbzac5mhjnxpkxle 22 7 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 22 8 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 22 9 catalyzed catalyzed JJ work_infoczas7nfbzac5mhjnxpkxle 22 10 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 22 11 and and CC work_infoczas7nfbzac5mhjnxpkxle 22 12 at at RB work_infoczas7nfbzac5mhjnxpkxle 22 13 least least RBS work_infoczas7nfbzac5mhjnxpkxle 22 14 one one CD work_infoczas7nfbzac5mhjnxpkxle 22 15 copy copy NN work_infoczas7nfbzac5mhjnxpkxle 22 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 22 17 randomly randomly RB work_infoczas7nfbzac5mhjnxpkxle 22 18 inserted insert VBN work_infoczas7nfbzac5mhjnxpkxle 22 19 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 22 20 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 22 21 . . . work_infoczas7nfbzac5mhjnxpkxle 23 1 Our -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 23 2 findings finding NNS work_infoczas7nfbzac5mhjnxpkxle 23 3 illustrate illustrate VBP work_infoczas7nfbzac5mhjnxpkxle 23 4 critical critical JJ work_infoczas7nfbzac5mhjnxpkxle 23 5 issues issue NNS work_infoczas7nfbzac5mhjnxpkxle 23 6 related relate VBN work_infoczas7nfbzac5mhjnxpkxle 23 7 to to IN work_infoczas7nfbzac5mhjnxpkxle 23 8 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 23 9 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 23 10 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 23 11 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 23 12 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 23 13 , , , work_infoczas7nfbzac5mhjnxpkxle 23 14 including include VBG work_infoczas7nfbzac5mhjnxpkxle 23 15 coincidental coincidental NNP work_infoczas7nfbzac5mhjnxpkxle 23 16 plasmid plasmid NNP work_infoczas7nfbzac5mhjnxpkxle 23 17 insertion insertion NNP work_infoczas7nfbzac5mhjnxpkxle 23 18 and and CC work_infoczas7nfbzac5mhjnxpkxle 23 19 relatively relatively RB work_infoczas7nfbzac5mhjnxpkxle 23 20 low low JJ work_infoczas7nfbzac5mhjnxpkxle 23 21 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 23 22 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 23 23 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 23 24 activity activity NN work_infoczas7nfbzac5mhjnxpkxle 23 25 in in IN work_infoczas7nfbzac5mhjnxpkxle 23 26 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 23 27 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 23 28 , , , work_infoczas7nfbzac5mhjnxpkxle 23 29 but but CC work_infoczas7nfbzac5mhjnxpkxle 23 30 also also RB work_infoczas7nfbzac5mhjnxpkxle 23 31 provide provide VB work_infoczas7nfbzac5mhjnxpkxle 23 32 a a DT work_infoczas7nfbzac5mhjnxpkxle 23 33 platform platform NN work_infoczas7nfbzac5mhjnxpkxle 23 34 for for IN work_infoczas7nfbzac5mhjnxpkxle 23 35 future future JJ work_infoczas7nfbzac5mhjnxpkxle 23 36 development development NN work_infoczas7nfbzac5mhjnxpkxle 23 37 of of IN work_infoczas7nfbzac5mhjnxpkxle 23 38 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 23 39 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 23 40 models model NNS work_infoczas7nfbzac5mhjnxpkxle 23 41 using use VBG work_infoczas7nfbzac5mhjnxpkxle 23 42 the the DT work_infoczas7nfbzac5mhjnxpkxle 23 43 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 23 44 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 23 45 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 23 46 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 23 47 technology technology NN work_infoczas7nfbzac5mhjnxpkxle 23 48 . . . work_infoczas7nfbzac5mhjnxpkxle 24 1 . . . work_infoczas7nfbzac5mhjnxpkxle 25 1 3 3 CD work_infoczas7nfbzac5mhjnxpkxle 25 2 INTRODUCTION introduction NN work_infoczas7nfbzac5mhjnxpkxle 25 3 Disease Disease NNP work_infoczas7nfbzac5mhjnxpkxle 25 4 models model NNS work_infoczas7nfbzac5mhjnxpkxle 25 5 in in IN work_infoczas7nfbzac5mhjnxpkxle 25 6 large large JJ work_infoczas7nfbzac5mhjnxpkxle 25 7 animals animal NNS work_infoczas7nfbzac5mhjnxpkxle 25 8 play play VBP work_infoczas7nfbzac5mhjnxpkxle 25 9 an an DT work_infoczas7nfbzac5mhjnxpkxle 25 10 increasing increase VBG work_infoczas7nfbzac5mhjnxpkxle 25 11 role role NN work_infoczas7nfbzac5mhjnxpkxle 25 12 in in IN work_infoczas7nfbzac5mhjnxpkxle 25 13 biomedical biomedical JJ work_infoczas7nfbzac5mhjnxpkxle 25 14 research research NN work_infoczas7nfbzac5mhjnxpkxle 25 15 and and CC work_infoczas7nfbzac5mhjnxpkxle 25 16 have have VBP work_infoczas7nfbzac5mhjnxpkxle 25 17 important important JJ work_infoczas7nfbzac5mhjnxpkxle 25 18 implications implication NNS work_infoczas7nfbzac5mhjnxpkxle 25 19 for for IN work_infoczas7nfbzac5mhjnxpkxle 25 20 the the DT work_infoczas7nfbzac5mhjnxpkxle 25 21 development development NN work_infoczas7nfbzac5mhjnxpkxle 25 22 of of IN work_infoczas7nfbzac5mhjnxpkxle 25 23 diagnostic diagnostic JJ work_infoczas7nfbzac5mhjnxpkxle 25 24 tools tool NNS work_infoczas7nfbzac5mhjnxpkxle 25 25 and and CC work_infoczas7nfbzac5mhjnxpkxle 25 26 novel novel JJ work_infoczas7nfbzac5mhjnxpkxle 25 27 treatment treatment NN work_infoczas7nfbzac5mhjnxpkxle 25 28 of of IN work_infoczas7nfbzac5mhjnxpkxle 25 29 human human JJ work_infoczas7nfbzac5mhjnxpkxle 25 30 diseases disease NNS work_infoczas7nfbzac5mhjnxpkxle 25 31 . . . work_infoczas7nfbzac5mhjnxpkxle 26 1 Due due IN work_infoczas7nfbzac5mhjnxpkxle 26 2 to to IN work_infoczas7nfbzac5mhjnxpkxle 26 3 the the DT work_infoczas7nfbzac5mhjnxpkxle 26 4 anatomical anatomical JJ work_infoczas7nfbzac5mhjnxpkxle 26 5 and and CC work_infoczas7nfbzac5mhjnxpkxle 26 6 physiological physiological JJ work_infoczas7nfbzac5mhjnxpkxle 26 7 similarities similarity NNS work_infoczas7nfbzac5mhjnxpkxle 26 8 between between IN work_infoczas7nfbzac5mhjnxpkxle 26 9 humans human NNS work_infoczas7nfbzac5mhjnxpkxle 26 10 and and CC work_infoczas7nfbzac5mhjnxpkxle 26 11 the the DT work_infoczas7nfbzac5mhjnxpkxle 26 12 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 26 13 , , , work_infoczas7nfbzac5mhjnxpkxle 26 14 genetically genetically RB work_infoczas7nfbzac5mhjnxpkxle 26 15 engineered engineer VBN work_infoczas7nfbzac5mhjnxpkxle 26 16 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 26 17 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 26 18 models model NNS work_infoczas7nfbzac5mhjnxpkxle 26 19 may may MD work_infoczas7nfbzac5mhjnxpkxle 26 20 be be VB work_infoczas7nfbzac5mhjnxpkxle 26 21 expected expect VBN work_infoczas7nfbzac5mhjnxpkxle 26 22 to to TO work_infoczas7nfbzac5mhjnxpkxle 26 23 recapitulate recapitulate VB work_infoczas7nfbzac5mhjnxpkxle 26 24 human human JJ work_infoczas7nfbzac5mhjnxpkxle 26 25 pathophysiology pathophysiology NN work_infoczas7nfbzac5mhjnxpkxle 26 26 more more RBR work_infoczas7nfbzac5mhjnxpkxle 26 27 faithfully faithfully RB work_infoczas7nfbzac5mhjnxpkxle 26 28 than than IN work_infoczas7nfbzac5mhjnxpkxle 26 29 do do VB work_infoczas7nfbzac5mhjnxpkxle 26 30 corresponding correspond VBG work_infoczas7nfbzac5mhjnxpkxle 26 31 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 26 32 models model NNS work_infoczas7nfbzac5mhjnxpkxle 26 33 in in IN work_infoczas7nfbzac5mhjnxpkxle 26 34 rodents rodent NNS work_infoczas7nfbzac5mhjnxpkxle 26 35 . . . work_infoczas7nfbzac5mhjnxpkxle 27 1 Also also RB work_infoczas7nfbzac5mhjnxpkxle 27 2 , , , work_infoczas7nfbzac5mhjnxpkxle 27 3 a a DT work_infoczas7nfbzac5mhjnxpkxle 27 4 longer long JJR work_infoczas7nfbzac5mhjnxpkxle 27 5 life life NN work_infoczas7nfbzac5mhjnxpkxle 27 6 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 27 7 span span NN work_infoczas7nfbzac5mhjnxpkxle 27 8 may may MD work_infoczas7nfbzac5mhjnxpkxle 27 9 allow allow VB work_infoczas7nfbzac5mhjnxpkxle 27 10 studies study NNS work_infoczas7nfbzac5mhjnxpkxle 27 11 of of IN work_infoczas7nfbzac5mhjnxpkxle 27 12 chronic chronic JJ work_infoczas7nfbzac5mhjnxpkxle 27 13 diseases disease NNS work_infoczas7nfbzac5mhjnxpkxle 27 14 , , , work_infoczas7nfbzac5mhjnxpkxle 27 15 and and CC work_infoczas7nfbzac5mhjnxpkxle 27 16 a a DT work_infoczas7nfbzac5mhjnxpkxle 27 17 human human JJ work_infoczas7nfbzac5mhjnxpkxle 27 18 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 27 19 size size NN work_infoczas7nfbzac5mhjnxpkxle 27 20 body body NN work_infoczas7nfbzac5mhjnxpkxle 27 21 may may MD work_infoczas7nfbzac5mhjnxpkxle 27 22 enable enable VB work_infoczas7nfbzac5mhjnxpkxle 27 23 studies study NNS work_infoczas7nfbzac5mhjnxpkxle 27 24 of of IN work_infoczas7nfbzac5mhjnxpkxle 27 25 surgical surgical JJ work_infoczas7nfbzac5mhjnxpkxle 27 26 intervention intervention NN work_infoczas7nfbzac5mhjnxpkxle 27 27 . . . work_infoczas7nfbzac5mhjnxpkxle 28 1 Pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 28 2 that that WDT work_infoczas7nfbzac5mhjnxpkxle 28 3 are be VBP work_infoczas7nfbzac5mhjnxpkxle 28 4 predisposed predispose VBN work_infoczas7nfbzac5mhjnxpkxle 28 5 for for IN work_infoczas7nfbzac5mhjnxpkxle 28 6 a a DT work_infoczas7nfbzac5mhjnxpkxle 28 7 certain certain JJ work_infoczas7nfbzac5mhjnxpkxle 28 8 monogenic monogenic JJ work_infoczas7nfbzac5mhjnxpkxle 28 9 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 28 10 can can MD work_infoczas7nfbzac5mhjnxpkxle 28 11 be be VB work_infoczas7nfbzac5mhjnxpkxle 28 12 created create VBN work_infoczas7nfbzac5mhjnxpkxle 28 13 by by IN work_infoczas7nfbzac5mhjnxpkxle 28 14 genetic genetic JJ work_infoczas7nfbzac5mhjnxpkxle 28 15 engineering engineering NN work_infoczas7nfbzac5mhjnxpkxle 28 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 28 17 the the DT work_infoczas7nfbzac5mhjnxpkxle 28 18 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 28 19 genome genome NNP work_infoczas7nfbzac5mhjnxpkxle 28 20 , , , work_infoczas7nfbzac5mhjnxpkxle 28 21 in in IN work_infoczas7nfbzac5mhjnxpkxle 28 22 the the DT work_infoczas7nfbzac5mhjnxpkxle 28 23 least least JJS work_infoczas7nfbzac5mhjnxpkxle 28 24 complicated complicated JJ work_infoczas7nfbzac5mhjnxpkxle 28 25 approach approach NN work_infoczas7nfbzac5mhjnxpkxle 28 26 by by IN work_infoczas7nfbzac5mhjnxpkxle 28 27 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 28 28 of of IN work_infoczas7nfbzac5mhjnxpkxle 28 29 a a DT work_infoczas7nfbzac5mhjnxpkxle 28 30 proposed propose VBN work_infoczas7nfbzac5mhjnxpkxle 28 31 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 28 32 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 28 33 which which WDT work_infoczas7nfbzac5mhjnxpkxle 28 34 may may MD work_infoczas7nfbzac5mhjnxpkxle 28 35 in in IN work_infoczas7nfbzac5mhjnxpkxle 28 36 a a DT work_infoczas7nfbzac5mhjnxpkxle 28 37 dominant dominant JJ work_infoczas7nfbzac5mhjnxpkxle 28 38 fashion fashion NN work_infoczas7nfbzac5mhjnxpkxle 28 39 trigger trigger NN work_infoczas7nfbzac5mhjnxpkxle 28 40 development development NN work_infoczas7nfbzac5mhjnxpkxle 28 41 of of IN work_infoczas7nfbzac5mhjnxpkxle 28 42 the the DT work_infoczas7nfbzac5mhjnxpkxle 28 43 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 28 44 . . . work_infoczas7nfbzac5mhjnxpkxle 29 1 Transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 29 2 in in IN work_infoczas7nfbzac5mhjnxpkxle 29 3 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 29 4 has have VBZ work_infoczas7nfbzac5mhjnxpkxle 29 5 previously previously RB work_infoczas7nfbzac5mhjnxpkxle 29 6 been be VBN work_infoczas7nfbzac5mhjnxpkxle 29 7 achieved achieve VBN work_infoczas7nfbzac5mhjnxpkxle 29 8 by by IN work_infoczas7nfbzac5mhjnxpkxle 29 9 transferring transfer VBG work_infoczas7nfbzac5mhjnxpkxle 29 10 the the DT work_infoczas7nfbzac5mhjnxpkxle 29 11 transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 29 12 to to IN work_infoczas7nfbzac5mhjnxpkxle 29 13 oocytes oocyte NNS work_infoczas7nfbzac5mhjnxpkxle 29 14 or or CC work_infoczas7nfbzac5mhjnxpkxle 29 15 early early JJ work_infoczas7nfbzac5mhjnxpkxle 29 16 embryos embryo NNS work_infoczas7nfbzac5mhjnxpkxle 29 17 by by IN work_infoczas7nfbzac5mhjnxpkxle 29 18 pronuclear pronuclear NN work_infoczas7nfbzac5mhjnxpkxle 29 19 injection injection NN work_infoczas7nfbzac5mhjnxpkxle 29 20 of of IN work_infoczas7nfbzac5mhjnxpkxle 29 21 foreign foreign JJ work_infoczas7nfbzac5mhjnxpkxle 29 22 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 29 23 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 29 24 Hammer Hammer NNP work_infoczas7nfbzac5mhjnxpkxle 29 25 et et NNP work_infoczas7nfbzac5mhjnxpkxle 29 26 al al NNP work_infoczas7nfbzac5mhjnxpkxle 29 27 . . . work_infoczas7nfbzac5mhjnxpkxle 30 1 1985 1985 CD work_infoczas7nfbzac5mhjnxpkxle 30 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 30 3 Hirabayashi Hirabayashi NNP work_infoczas7nfbzac5mhjnxpkxle 30 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 30 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 30 6 . . . work_infoczas7nfbzac5mhjnxpkxle 31 1 2001 2001 CD work_infoczas7nfbzac5mhjnxpkxle 31 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 31 3 Nottle Nottle NNP work_infoczas7nfbzac5mhjnxpkxle 31 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 31 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 31 6 . . . work_infoczas7nfbzac5mhjnxpkxle 32 1 2001 2001 CD work_infoczas7nfbzac5mhjnxpkxle 32 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 32 3 , , , work_infoczas7nfbzac5mhjnxpkxle 32 4 transduction transduction NN work_infoczas7nfbzac5mhjnxpkxle 32 5 with with IN work_infoczas7nfbzac5mhjnxpkxle 32 6 retro- retro- NN work_infoczas7nfbzac5mhjnxpkxle 32 7 and and CC work_infoczas7nfbzac5mhjnxpkxle 32 8 lentiviral lentiviral JJ work_infoczas7nfbzac5mhjnxpkxle 32 9 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 32 10 injected inject VBN work_infoczas7nfbzac5mhjnxpkxle 32 11 into into IN work_infoczas7nfbzac5mhjnxpkxle 32 12 the the DT work_infoczas7nfbzac5mhjnxpkxle 32 13 perivitelline perivitelline NN work_infoczas7nfbzac5mhjnxpkxle 32 14 space space NN work_infoczas7nfbzac5mhjnxpkxle 32 15 of of IN work_infoczas7nfbzac5mhjnxpkxle 32 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 32 17 oocyte oocyte NN work_infoczas7nfbzac5mhjnxpkxle 32 18 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 32 19 Cabot Cabot NNP work_infoczas7nfbzac5mhjnxpkxle 32 20 et et NNP work_infoczas7nfbzac5mhjnxpkxle 32 21 al al NNP work_infoczas7nfbzac5mhjnxpkxle 32 22 . . . work_infoczas7nfbzac5mhjnxpkxle 33 1 2001 2001 CD work_infoczas7nfbzac5mhjnxpkxle 33 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 33 3 Hofmann Hofmann NNP work_infoczas7nfbzac5mhjnxpkxle 33 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 33 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 33 6 . . . work_infoczas7nfbzac5mhjnxpkxle 34 1 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 34 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 34 3 Whitelaw Whitelaw NNP work_infoczas7nfbzac5mhjnxpkxle 34 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 34 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 34 6 . . . work_infoczas7nfbzac5mhjnxpkxle 35 1 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 35 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 35 3 or or CC work_infoczas7nfbzac5mhjnxpkxle 35 4 , , , work_infoczas7nfbzac5mhjnxpkxle 35 5 alternatively alternatively RB work_infoczas7nfbzac5mhjnxpkxle 35 6 , , , work_infoczas7nfbzac5mhjnxpkxle 35 7 by by IN work_infoczas7nfbzac5mhjnxpkxle 35 8 sperm sperm NN work_infoczas7nfbzac5mhjnxpkxle 35 9 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 35 10 mediated mediate VBN work_infoczas7nfbzac5mhjnxpkxle 35 11 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 35 12 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 35 13 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 35 14 Naruse Naruse NNP work_infoczas7nfbzac5mhjnxpkxle 35 15 et et NNP work_infoczas7nfbzac5mhjnxpkxle 35 16 al al NNP work_infoczas7nfbzac5mhjnxpkxle 35 17 . . . work_infoczas7nfbzac5mhjnxpkxle 36 1 2005 2005 CD work_infoczas7nfbzac5mhjnxpkxle 36 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 36 3 Webster Webster NNP work_infoczas7nfbzac5mhjnxpkxle 36 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 36 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 36 6 . . . work_infoczas7nfbzac5mhjnxpkxle 37 1 2005 2005 CD work_infoczas7nfbzac5mhjnxpkxle 37 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 37 3 . . . work_infoczas7nfbzac5mhjnxpkxle 38 1 With with IN work_infoczas7nfbzac5mhjnxpkxle 38 2 the the DT work_infoczas7nfbzac5mhjnxpkxle 38 3 successful successful JJ work_infoczas7nfbzac5mhjnxpkxle 38 4 cloning cloning NN work_infoczas7nfbzac5mhjnxpkxle 38 5 of of IN work_infoczas7nfbzac5mhjnxpkxle 38 6 animals animal NNS work_infoczas7nfbzac5mhjnxpkxle 38 7 by by IN work_infoczas7nfbzac5mhjnxpkxle 38 8 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 38 9 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 38 10 nuclear nuclear JJ work_infoczas7nfbzac5mhjnxpkxle 38 11 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 38 12 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 38 13 SCNT SCNT NNP work_infoczas7nfbzac5mhjnxpkxle 38 14 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 38 15 , , , work_infoczas7nfbzac5mhjnxpkxle 38 16 it -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 38 17 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 38 18 now now RB work_infoczas7nfbzac5mhjnxpkxle 38 19 possible possible JJ work_infoczas7nfbzac5mhjnxpkxle 38 20 to to TO work_infoczas7nfbzac5mhjnxpkxle 38 21 produce produce VB work_infoczas7nfbzac5mhjnxpkxle 38 22 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 38 23 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 38 24 from from IN work_infoczas7nfbzac5mhjnxpkxle 38 25 genetically genetically RB work_infoczas7nfbzac5mhjnxpkxle 38 26 engineered engineer VBN work_infoczas7nfbzac5mhjnxpkxle 38 27 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 38 28 donor donor NN work_infoczas7nfbzac5mhjnxpkxle 38 29 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 38 30 . . . work_infoczas7nfbzac5mhjnxpkxle 39 1 In in IN work_infoczas7nfbzac5mhjnxpkxle 39 2 SCNT SCNT NNP work_infoczas7nfbzac5mhjnxpkxle 39 3 , , , work_infoczas7nfbzac5mhjnxpkxle 39 4 reported report VBD work_infoczas7nfbzac5mhjnxpkxle 39 5 for for IN work_infoczas7nfbzac5mhjnxpkxle 39 6 a a DT work_infoczas7nfbzac5mhjnxpkxle 39 7 variety variety NN work_infoczas7nfbzac5mhjnxpkxle 39 8 of of IN work_infoczas7nfbzac5mhjnxpkxle 39 9 animal animal NN work_infoczas7nfbzac5mhjnxpkxle 39 10 species specie NNS work_infoczas7nfbzac5mhjnxpkxle 39 11 including include VBG work_infoczas7nfbzac5mhjnxpkxle 39 12 the the DT work_infoczas7nfbzac5mhjnxpkxle 39 13 mouse mouse NN work_infoczas7nfbzac5mhjnxpkxle 39 14 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 39 15 Wakayama Wakayama NNP work_infoczas7nfbzac5mhjnxpkxle 39 16 et et FW work_infoczas7nfbzac5mhjnxpkxle 39 17 al al NNP work_infoczas7nfbzac5mhjnxpkxle 39 18 . . . work_infoczas7nfbzac5mhjnxpkxle 40 1 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 40 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 40 3 Wakayama Wakayama NNP work_infoczas7nfbzac5mhjnxpkxle 40 4 and and CC work_infoczas7nfbzac5mhjnxpkxle 40 5 Yanagimachi Yanagimachi NNP work_infoczas7nfbzac5mhjnxpkxle 40 6 1999 1999 CD work_infoczas7nfbzac5mhjnxpkxle 40 7 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 40 8 , , , work_infoczas7nfbzac5mhjnxpkxle 40 9 the the DT work_infoczas7nfbzac5mhjnxpkxle 40 10 sheep sheep NN work_infoczas7nfbzac5mhjnxpkxle 40 11 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 40 12 Wilmut Wilmut NNP work_infoczas7nfbzac5mhjnxpkxle 40 13 et et NNP work_infoczas7nfbzac5mhjnxpkxle 40 14 al al NNP work_infoczas7nfbzac5mhjnxpkxle 40 15 . . . work_infoczas7nfbzac5mhjnxpkxle 41 1 1997 1997 CD work_infoczas7nfbzac5mhjnxpkxle 41 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 41 3 , , , work_infoczas7nfbzac5mhjnxpkxle 41 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 41 5 cow cow NN work_infoczas7nfbzac5mhjnxpkxle 41 6 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 41 7 Cibelli Cibelli NNP work_infoczas7nfbzac5mhjnxpkxle 41 8 et et FW work_infoczas7nfbzac5mhjnxpkxle 41 9 al al NNP work_infoczas7nfbzac5mhjnxpkxle 41 10 . . . work_infoczas7nfbzac5mhjnxpkxle 42 1 1998 1998 CD work_infoczas7nfbzac5mhjnxpkxle 42 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 42 3 Kato Kato NNP work_infoczas7nfbzac5mhjnxpkxle 42 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 42 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 42 6 . . . work_infoczas7nfbzac5mhjnxpkxle 43 1 1998 1998 CD work_infoczas7nfbzac5mhjnxpkxle 43 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 43 3 Kubota Kubota NNP work_infoczas7nfbzac5mhjnxpkxle 43 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 43 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 43 6 . . . work_infoczas7nfbzac5mhjnxpkxle 44 1 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 44 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 44 3 , , , work_infoczas7nfbzac5mhjnxpkxle 44 4 and and CC work_infoczas7nfbzac5mhjnxpkxle 44 5 the the DT work_infoczas7nfbzac5mhjnxpkxle 44 6 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 44 7 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 44 8 Betthauser Betthauser NNP work_infoczas7nfbzac5mhjnxpkxle 44 9 et et NNP work_infoczas7nfbzac5mhjnxpkxle 44 10 al al NNP work_infoczas7nfbzac5mhjnxpkxle 44 11 . . . work_infoczas7nfbzac5mhjnxpkxle 45 1 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 45 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 45 3 Onishi Onishi NNP work_infoczas7nfbzac5mhjnxpkxle 45 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 45 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 45 6 . . . work_infoczas7nfbzac5mhjnxpkxle 46 1 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 46 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 46 3 Polejaeva Polejaeva NNP work_infoczas7nfbzac5mhjnxpkxle 46 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 46 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 46 6 . . . work_infoczas7nfbzac5mhjnxpkxle 47 1 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 47 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 47 3 , , , work_infoczas7nfbzac5mhjnxpkxle 47 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 47 5 nucleus nucleus NN work_infoczas7nfbzac5mhjnxpkxle 47 6 from from IN work_infoczas7nfbzac5mhjnxpkxle 47 7 a a DT work_infoczas7nfbzac5mhjnxpkxle 47 8 single single JJ work_infoczas7nfbzac5mhjnxpkxle 47 9 differentiated differentiated JJ work_infoczas7nfbzac5mhjnxpkxle 47 10 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 47 11 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 47 12 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 47 13 transferred transfer VBN work_infoczas7nfbzac5mhjnxpkxle 47 14 into into IN work_infoczas7nfbzac5mhjnxpkxle 47 15 an an DT work_infoczas7nfbzac5mhjnxpkxle 47 16 enucleated enucleate VBN work_infoczas7nfbzac5mhjnxpkxle 47 17 oocyte oocyte NN work_infoczas7nfbzac5mhjnxpkxle 47 18 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 47 19 unfertilized unfertilized JJ work_infoczas7nfbzac5mhjnxpkxle 47 20 egg egg NN work_infoczas7nfbzac5mhjnxpkxle 47 21 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 47 22 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 47 23 , , , work_infoczas7nfbzac5mhjnxpkxle 47 24 and and CC work_infoczas7nfbzac5mhjnxpkxle 47 25 the the DT work_infoczas7nfbzac5mhjnxpkxle 47 26 reconstructed reconstruct VBN work_infoczas7nfbzac5mhjnxpkxle 47 27 embryo embryo NN work_infoczas7nfbzac5mhjnxpkxle 47 28 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 47 29 subsequently subsequently RB work_infoczas7nfbzac5mhjnxpkxle 47 30 transferred transfer VBN work_infoczas7nfbzac5mhjnxpkxle 47 31 to to IN work_infoczas7nfbzac5mhjnxpkxle 47 32 a a DT work_infoczas7nfbzac5mhjnxpkxle 47 33 surrogate surrogate JJ work_infoczas7nfbzac5mhjnxpkxle 47 34 mother mother NN work_infoczas7nfbzac5mhjnxpkxle 47 35 . . . work_infoczas7nfbzac5mhjnxpkxle 48 1 This this DT work_infoczas7nfbzac5mhjnxpkxle 48 2 procedure procedure NN work_infoczas7nfbzac5mhjnxpkxle 48 3 allows allow VBZ work_infoczas7nfbzac5mhjnxpkxle 48 4 modification modification NN work_infoczas7nfbzac5mhjnxpkxle 48 5 of of IN work_infoczas7nfbzac5mhjnxpkxle 48 6 the the DT work_infoczas7nfbzac5mhjnxpkxle 48 7 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 48 8 donor donor NN work_infoczas7nfbzac5mhjnxpkxle 48 9 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 48 10 in in IN work_infoczas7nfbzac5mhjnxpkxle 48 11 culture culture NN work_infoczas7nfbzac5mhjnxpkxle 48 12 by by IN work_infoczas7nfbzac5mhjnxpkxle 48 13 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 48 14 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 48 15 or or CC work_infoczas7nfbzac5mhjnxpkxle 48 16 introduction introduction NN work_infoczas7nfbzac5mhjnxpkxle 48 17 of of IN work_infoczas7nfbzac5mhjnxpkxle 48 18 loss loss NN work_infoczas7nfbzac5mhjnxpkxle 48 19 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 48 20 of- of- CD work_infoczas7nfbzac5mhjnxpkxle 48 21 function function NN work_infoczas7nfbzac5mhjnxpkxle 48 22 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 48 23 knockout knockout NN work_infoczas7nfbzac5mhjnxpkxle 48 24 mutations mutation NNS work_infoczas7nfbzac5mhjnxpkxle 48 25 by by IN work_infoczas7nfbzac5mhjnxpkxle 48 26 homologous homologous JJ work_infoczas7nfbzac5mhjnxpkxle 48 27 recombination recombination NN work_infoczas7nfbzac5mhjnxpkxle 48 28 . . . work_infoczas7nfbzac5mhjnxpkxle 49 1 We -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 49 2 have have VBP work_infoczas7nfbzac5mhjnxpkxle 49 3 previously previously RB work_infoczas7nfbzac5mhjnxpkxle 49 4 produced produce VBN work_infoczas7nfbzac5mhjnxpkxle 49 5 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 49 6 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 49 7 blastocysts blastocyst NNS work_infoczas7nfbzac5mhjnxpkxle 49 8 by by IN work_infoczas7nfbzac5mhjnxpkxle 49 9 SCNT SCNT NNP work_infoczas7nfbzac5mhjnxpkxle 49 10 using use VBG work_infoczas7nfbzac5mhjnxpkxle 49 11 a a DT work_infoczas7nfbzac5mhjnxpkxle 49 12 simplified simplify VBN work_infoczas7nfbzac5mhjnxpkxle 49 13 and and CC work_infoczas7nfbzac5mhjnxpkxle 49 14 improved improve VBN work_infoczas7nfbzac5mhjnxpkxle 49 15 cloning clone VBG work_infoczas7nfbzac5mhjnxpkxle 49 16 procedure procedure NN work_infoczas7nfbzac5mhjnxpkxle 49 17 designated designate VBD work_infoczas7nfbzac5mhjnxpkxle 49 18 4 4 CD work_infoczas7nfbzac5mhjnxpkxle 49 19 handmade handmade NN work_infoczas7nfbzac5mhjnxpkxle 49 20 cloning cloning NN work_infoczas7nfbzac5mhjnxpkxle 49 21 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 49 22 HMC HMC NNP work_infoczas7nfbzac5mhjnxpkxle 49 23 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 49 24 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 49 25 Du Du NNP work_infoczas7nfbzac5mhjnxpkxle 49 26 et et NNP work_infoczas7nfbzac5mhjnxpkxle 49 27 al al NNP work_infoczas7nfbzac5mhjnxpkxle 49 28 . . . work_infoczas7nfbzac5mhjnxpkxle 50 1 2007 2007 CD work_infoczas7nfbzac5mhjnxpkxle 50 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 50 3 Kragh Kragh NNP work_infoczas7nfbzac5mhjnxpkxle 50 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 50 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 50 6 . . . work_infoczas7nfbzac5mhjnxpkxle 51 1 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 51 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 51 3 and and CC work_infoczas7nfbzac5mhjnxpkxle 51 4 recently recently RB work_infoczas7nfbzac5mhjnxpkxle 51 5 developed develop VBD work_infoczas7nfbzac5mhjnxpkxle 51 6 Göttingen Göttingen NNP work_infoczas7nfbzac5mhjnxpkxle 51 7 minipigs minipig NNS work_infoczas7nfbzac5mhjnxpkxle 51 8 expressing express VBG work_infoczas7nfbzac5mhjnxpkxle 51 9 a a DT work_infoczas7nfbzac5mhjnxpkxle 51 10 randomly randomly RB work_infoczas7nfbzac5mhjnxpkxle 51 11 inserted insert VBN work_infoczas7nfbzac5mhjnxpkxle 51 12 human human JJ work_infoczas7nfbzac5mhjnxpkxle 51 13 APP APP NNP work_infoczas7nfbzac5mhjnxpkxle 51 14 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 51 15 containing contain VBG work_infoczas7nfbzac5mhjnxpkxle 51 16 an an DT work_infoczas7nfbzac5mhjnxpkxle 51 17 Alzheimer Alzheimer NNP work_infoczas7nfbzac5mhjnxpkxle 51 18 ’s ’s POS work_infoczas7nfbzac5mhjnxpkxle 51 19 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 51 20 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 51 21 causing cause VBG work_infoczas7nfbzac5mhjnxpkxle 51 22 dominant dominant JJ work_infoczas7nfbzac5mhjnxpkxle 51 23 mutation mutation NN work_infoczas7nfbzac5mhjnxpkxle 51 24 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 51 25 Kragh Kragh NNP work_infoczas7nfbzac5mhjnxpkxle 51 26 et et FW work_infoczas7nfbzac5mhjnxpkxle 51 27 al al NNP work_infoczas7nfbzac5mhjnxpkxle 51 28 . . . work_infoczas7nfbzac5mhjnxpkxle 52 1 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 52 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 52 3 . . . work_infoczas7nfbzac5mhjnxpkxle 53 1 Permanent permanent JJ work_infoczas7nfbzac5mhjnxpkxle 53 2 genetic genetic JJ work_infoczas7nfbzac5mhjnxpkxle 53 3 modification modification NN work_infoczas7nfbzac5mhjnxpkxle 53 4 of of IN work_infoczas7nfbzac5mhjnxpkxle 53 5 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 53 6 donor donor NN work_infoczas7nfbzac5mhjnxpkxle 53 7 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 53 8 for for IN work_infoczas7nfbzac5mhjnxpkxle 53 9 SCNT SCNT NNP work_infoczas7nfbzac5mhjnxpkxle 53 10 has have VBZ work_infoczas7nfbzac5mhjnxpkxle 53 11 been be VBN work_infoczas7nfbzac5mhjnxpkxle 53 12 achieved achieve VBN work_infoczas7nfbzac5mhjnxpkxle 53 13 by by IN work_infoczas7nfbzac5mhjnxpkxle 53 14 random random JJ work_infoczas7nfbzac5mhjnxpkxle 53 15 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 53 16 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 53 17 of of IN work_infoczas7nfbzac5mhjnxpkxle 53 18 plasmid plasmid NNP work_infoczas7nfbzac5mhjnxpkxle 53 19 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 53 20 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 53 21 Hyun Hyun NNP work_infoczas7nfbzac5mhjnxpkxle 53 22 et et FW work_infoczas7nfbzac5mhjnxpkxle 53 23 al al NNP work_infoczas7nfbzac5mhjnxpkxle 53 24 . . . work_infoczas7nfbzac5mhjnxpkxle 54 1 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 54 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 54 3 Kragh Kragh NNP work_infoczas7nfbzac5mhjnxpkxle 54 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 54 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 54 6 . . . work_infoczas7nfbzac5mhjnxpkxle 55 1 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 55 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 55 3 Kragh Kragh NNP work_infoczas7nfbzac5mhjnxpkxle 55 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 55 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 55 6 . . . work_infoczas7nfbzac5mhjnxpkxle 56 1 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 56 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 56 3 Watanabe Watanabe NNP work_infoczas7nfbzac5mhjnxpkxle 56 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 56 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 56 6 . . . work_infoczas7nfbzac5mhjnxpkxle 57 1 2005 2005 LS work_infoczas7nfbzac5mhjnxpkxle 57 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 57 3 or or CC work_infoczas7nfbzac5mhjnxpkxle 57 4 by by IN work_infoczas7nfbzac5mhjnxpkxle 57 5 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 57 6 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 57 7 of of IN work_infoczas7nfbzac5mhjnxpkxle 57 8 transduced transduce VBN work_infoczas7nfbzac5mhjnxpkxle 57 9 retroviral retroviral JJ work_infoczas7nfbzac5mhjnxpkxle 57 10 or or CC work_infoczas7nfbzac5mhjnxpkxle 57 11 lentiviral lentiviral JJ work_infoczas7nfbzac5mhjnxpkxle 57 12 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 57 13 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 57 14 Lai Lai NNP work_infoczas7nfbzac5mhjnxpkxle 57 15 et et NNP work_infoczas7nfbzac5mhjnxpkxle 57 16 al al NNP work_infoczas7nfbzac5mhjnxpkxle 57 17 . . . work_infoczas7nfbzac5mhjnxpkxle 58 1 2002 2002 CD work_infoczas7nfbzac5mhjnxpkxle 58 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 58 3 Park Park NNP work_infoczas7nfbzac5mhjnxpkxle 58 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 58 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 58 6 . . . work_infoczas7nfbzac5mhjnxpkxle 59 1 2001 2001 CD work_infoczas7nfbzac5mhjnxpkxle 59 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 59 3 Park Park NNP work_infoczas7nfbzac5mhjnxpkxle 59 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 59 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 59 6 . . . work_infoczas7nfbzac5mhjnxpkxle 60 1 2002 2002 CD work_infoczas7nfbzac5mhjnxpkxle 60 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 60 3 . . . work_infoczas7nfbzac5mhjnxpkxle 61 1 In in IN work_infoczas7nfbzac5mhjnxpkxle 61 2 both both DT work_infoczas7nfbzac5mhjnxpkxle 61 3 non non JJ work_infoczas7nfbzac5mhjnxpkxle 61 4 - - JJ work_infoczas7nfbzac5mhjnxpkxle 61 5 viral viral JJ work_infoczas7nfbzac5mhjnxpkxle 61 6 and and CC work_infoczas7nfbzac5mhjnxpkxle 61 7 viral viral JJ work_infoczas7nfbzac5mhjnxpkxle 61 8 approaches approach NNS work_infoczas7nfbzac5mhjnxpkxle 61 9 inclusion inclusion NN work_infoczas7nfbzac5mhjnxpkxle 61 10 of of IN work_infoczas7nfbzac5mhjnxpkxle 61 11 a a DT work_infoczas7nfbzac5mhjnxpkxle 61 12 drug drug NN work_infoczas7nfbzac5mhjnxpkxle 61 13 resistance resistance NN work_infoczas7nfbzac5mhjnxpkxle 61 14 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 61 15 in in IN work_infoczas7nfbzac5mhjnxpkxle 61 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 61 17 transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 61 18 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 61 19 encoding encode VBG work_infoczas7nfbzac5mhjnxpkxle 61 20 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 61 21 allows allow VBZ work_infoczas7nfbzac5mhjnxpkxle 61 22 selection selection NN work_infoczas7nfbzac5mhjnxpkxle 61 23 for for IN work_infoczas7nfbzac5mhjnxpkxle 61 24 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 61 25 containing contain VBG work_infoczas7nfbzac5mhjnxpkxle 61 26 the the DT work_infoczas7nfbzac5mhjnxpkxle 61 27 integrated integrated JJ work_infoczas7nfbzac5mhjnxpkxle 61 28 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 61 29 . . . work_infoczas7nfbzac5mhjnxpkxle 62 1 Random random JJ work_infoczas7nfbzac5mhjnxpkxle 62 2 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 62 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 62 4 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 62 5 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 62 6 into into IN work_infoczas7nfbzac5mhjnxpkxle 62 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 62 8 genome genome NN work_infoczas7nfbzac5mhjnxpkxle 62 9 of of IN work_infoczas7nfbzac5mhjnxpkxle 62 10 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 62 11 donor donor NN work_infoczas7nfbzac5mhjnxpkxle 62 12 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 62 13 occurs occur VBZ work_infoczas7nfbzac5mhjnxpkxle 62 14 as as IN work_infoczas7nfbzac5mhjnxpkxle 62 15 a a DT work_infoczas7nfbzac5mhjnxpkxle 62 16 result result NN work_infoczas7nfbzac5mhjnxpkxle 62 17 of of IN work_infoczas7nfbzac5mhjnxpkxle 62 18 breakage breakage NN work_infoczas7nfbzac5mhjnxpkxle 62 19 of of IN work_infoczas7nfbzac5mhjnxpkxle 62 20 the the DT work_infoczas7nfbzac5mhjnxpkxle 62 21 plasmid plasmid NNP work_infoczas7nfbzac5mhjnxpkxle 62 22 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 62 23 . . . work_infoczas7nfbzac5mhjnxpkxle 63 1 Breakage breakage NN work_infoczas7nfbzac5mhjnxpkxle 63 2 within within IN work_infoczas7nfbzac5mhjnxpkxle 63 3 the the DT work_infoczas7nfbzac5mhjnxpkxle 63 4 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 63 5 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 63 6 may may MD work_infoczas7nfbzac5mhjnxpkxle 63 7 result result VB work_infoczas7nfbzac5mhjnxpkxle 63 8 in in IN work_infoczas7nfbzac5mhjnxpkxle 63 9 drug drug NN work_infoczas7nfbzac5mhjnxpkxle 63 10 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 63 11 selected select VBN work_infoczas7nfbzac5mhjnxpkxle 63 12 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 63 13 clones clone NNS work_infoczas7nfbzac5mhjnxpkxle 63 14 and and CC work_infoczas7nfbzac5mhjnxpkxle 63 15 eventually eventually RB work_infoczas7nfbzac5mhjnxpkxle 63 16 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 63 17 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 63 18 that that WDT work_infoczas7nfbzac5mhjnxpkxle 63 19 do do VBP work_infoczas7nfbzac5mhjnxpkxle 63 20 not not RB work_infoczas7nfbzac5mhjnxpkxle 63 21 express express VB work_infoczas7nfbzac5mhjnxpkxle 63 22 the the DT work_infoczas7nfbzac5mhjnxpkxle 63 23 transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 63 24 . . . work_infoczas7nfbzac5mhjnxpkxle 64 1 Moreover moreover RB work_infoczas7nfbzac5mhjnxpkxle 64 2 , , , work_infoczas7nfbzac5mhjnxpkxle 64 3 randomly randomly RB work_infoczas7nfbzac5mhjnxpkxle 64 4 inserted insert VBD work_infoczas7nfbzac5mhjnxpkxle 64 5 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 64 6 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 64 7 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 64 8 thought think VBN work_infoczas7nfbzac5mhjnxpkxle 64 9 to to TO work_infoczas7nfbzac5mhjnxpkxle 64 10 be be VB work_infoczas7nfbzac5mhjnxpkxle 64 11 more more RBR work_infoczas7nfbzac5mhjnxpkxle 64 12 prone prone JJ work_infoczas7nfbzac5mhjnxpkxle 64 13 to to IN work_infoczas7nfbzac5mhjnxpkxle 64 14 formation formation NN work_infoczas7nfbzac5mhjnxpkxle 64 15 of of IN work_infoczas7nfbzac5mhjnxpkxle 64 16 concatamers concatamer NNS work_infoczas7nfbzac5mhjnxpkxle 64 17 during during IN work_infoczas7nfbzac5mhjnxpkxle 64 18 the the DT work_infoczas7nfbzac5mhjnxpkxle 64 19 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 64 20 process process NN work_infoczas7nfbzac5mhjnxpkxle 64 21 which which WDT work_infoczas7nfbzac5mhjnxpkxle 64 22 may may MD work_infoczas7nfbzac5mhjnxpkxle 64 23 affect affect VB work_infoczas7nfbzac5mhjnxpkxle 64 24 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 64 25 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 64 26 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 64 27 Garrick Garrick NNP work_infoczas7nfbzac5mhjnxpkxle 64 28 et et FW work_infoczas7nfbzac5mhjnxpkxle 64 29 al al NNP work_infoczas7nfbzac5mhjnxpkxle 64 30 . . . work_infoczas7nfbzac5mhjnxpkxle 65 1 1998 1998 CD work_infoczas7nfbzac5mhjnxpkxle 65 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 65 3 Leahy Leahy NNP work_infoczas7nfbzac5mhjnxpkxle 65 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 65 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 65 6 . . . work_infoczas7nfbzac5mhjnxpkxle 66 1 1997 1997 CD work_infoczas7nfbzac5mhjnxpkxle 66 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 66 3 . . . work_infoczas7nfbzac5mhjnxpkxle 67 1 High high JJ work_infoczas7nfbzac5mhjnxpkxle 67 2 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 67 3 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 67 4 efficiency efficiency NN work_infoczas7nfbzac5mhjnxpkxle 67 5 and and CC work_infoczas7nfbzac5mhjnxpkxle 67 6 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 67 7 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 67 8 of of IN work_infoczas7nfbzac5mhjnxpkxle 67 9 a a DT work_infoczas7nfbzac5mhjnxpkxle 67 10 defined define VBN work_infoczas7nfbzac5mhjnxpkxle 67 11 genetic genetic JJ work_infoczas7nfbzac5mhjnxpkxle 67 12 entity entity NN work_infoczas7nfbzac5mhjnxpkxle 67 13 are be VBP work_infoczas7nfbzac5mhjnxpkxle 67 14 major major JJ work_infoczas7nfbzac5mhjnxpkxle 67 15 advantages advantage NNS work_infoczas7nfbzac5mhjnxpkxle 67 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 67 17 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 67 18 by by IN work_infoczas7nfbzac5mhjnxpkxle 67 19 retro- retro- JJ work_infoczas7nfbzac5mhjnxpkxle 67 20 and and CC work_infoczas7nfbzac5mhjnxpkxle 67 21 lentiviral lentiviral JJ work_infoczas7nfbzac5mhjnxpkxle 67 22 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 67 23 . . . work_infoczas7nfbzac5mhjnxpkxle 68 1 However however RB work_infoczas7nfbzac5mhjnxpkxle 68 2 , , , work_infoczas7nfbzac5mhjnxpkxle 68 3 both both DT work_infoczas7nfbzac5mhjnxpkxle 68 4 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 68 5 types type NNS work_infoczas7nfbzac5mhjnxpkxle 68 6 preferentially preferentially RB work_infoczas7nfbzac5mhjnxpkxle 68 7 target target VBP work_infoczas7nfbzac5mhjnxpkxle 68 8 transcriptionally transcriptionally RB work_infoczas7nfbzac5mhjnxpkxle 68 9 active active JJ work_infoczas7nfbzac5mhjnxpkxle 68 10 regions region NNS work_infoczas7nfbzac5mhjnxpkxle 68 11 of of IN work_infoczas7nfbzac5mhjnxpkxle 68 12 the the DT work_infoczas7nfbzac5mhjnxpkxle 68 13 genome genome JJ work_infoczas7nfbzac5mhjnxpkxle 68 14 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 68 15 Mitchell Mitchell NNP work_infoczas7nfbzac5mhjnxpkxle 68 16 et et FW work_infoczas7nfbzac5mhjnxpkxle 68 17 al al NNP work_infoczas7nfbzac5mhjnxpkxle 68 18 . . . work_infoczas7nfbzac5mhjnxpkxle 69 1 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 69 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 69 3 Schroder Schroder NNP work_infoczas7nfbzac5mhjnxpkxle 69 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 69 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 69 6 . . . work_infoczas7nfbzac5mhjnxpkxle 70 1 2002 2002 CD work_infoczas7nfbzac5mhjnxpkxle 70 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 70 3 and and CC work_infoczas7nfbzac5mhjnxpkxle 70 4 are be VBP work_infoczas7nfbzac5mhjnxpkxle 70 5 , , , work_infoczas7nfbzac5mhjnxpkxle 70 6 therefore therefore RB work_infoczas7nfbzac5mhjnxpkxle 70 7 , , , work_infoczas7nfbzac5mhjnxpkxle 70 8 likely likely JJ work_infoczas7nfbzac5mhjnxpkxle 70 9 to to TO work_infoczas7nfbzac5mhjnxpkxle 70 10 insert insert VB work_infoczas7nfbzac5mhjnxpkxle 70 11 near near RB work_infoczas7nfbzac5mhjnxpkxle 70 12 or or CC work_infoczas7nfbzac5mhjnxpkxle 70 13 into into IN work_infoczas7nfbzac5mhjnxpkxle 70 14 genes gene NNS work_infoczas7nfbzac5mhjnxpkxle 70 15 . . . work_infoczas7nfbzac5mhjnxpkxle 71 1 Also also RB work_infoczas7nfbzac5mhjnxpkxle 71 2 , , , work_infoczas7nfbzac5mhjnxpkxle 71 3 these these DT work_infoczas7nfbzac5mhjnxpkxle 71 4 technologies technology NNS work_infoczas7nfbzac5mhjnxpkxle 71 5 require require VBP work_infoczas7nfbzac5mhjnxpkxle 71 6 classified classified JJ work_infoczas7nfbzac5mhjnxpkxle 71 7 laboratory laboratory NN work_infoczas7nfbzac5mhjnxpkxle 71 8 facilities facility NNS work_infoczas7nfbzac5mhjnxpkxle 71 9 and and CC work_infoczas7nfbzac5mhjnxpkxle 71 10 extra extra JJ work_infoczas7nfbzac5mhjnxpkxle 71 11 precaution precaution NN work_infoczas7nfbzac5mhjnxpkxle 71 12 with with IN work_infoczas7nfbzac5mhjnxpkxle 71 13 respect respect NN work_infoczas7nfbzac5mhjnxpkxle 71 14 to to IN work_infoczas7nfbzac5mhjnxpkxle 71 15 housing housing NN work_infoczas7nfbzac5mhjnxpkxle 71 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 71 17 the the DT work_infoczas7nfbzac5mhjnxpkxle 71 18 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 71 19 animals animal NNS work_infoczas7nfbzac5mhjnxpkxle 71 20 . . . work_infoczas7nfbzac5mhjnxpkxle 72 1 In in IN work_infoczas7nfbzac5mhjnxpkxle 72 2 this this DT work_infoczas7nfbzac5mhjnxpkxle 72 3 report report NN work_infoczas7nfbzac5mhjnxpkxle 72 4 , , , work_infoczas7nfbzac5mhjnxpkxle 72 5 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 72 6 describe describe VBP work_infoczas7nfbzac5mhjnxpkxle 72 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 72 8 use use NN work_infoczas7nfbzac5mhjnxpkxle 72 9 of of IN work_infoczas7nfbzac5mhjnxpkxle 72 10 Sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 72 11 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 72 12 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 72 13 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 72 14 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 72 15 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 72 16 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 72 17 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 72 18 based base VBN work_infoczas7nfbzac5mhjnxpkxle 72 19 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 72 20 for for IN work_infoczas7nfbzac5mhjnxpkxle 72 21 creation creation NN work_infoczas7nfbzac5mhjnxpkxle 72 22 of of IN work_infoczas7nfbzac5mhjnxpkxle 72 23 genetically genetically RB work_infoczas7nfbzac5mhjnxpkxle 72 24 engineered engineer VBN work_infoczas7nfbzac5mhjnxpkxle 72 25 Göttingen Göttingen NNP work_infoczas7nfbzac5mhjnxpkxle 72 26 minipigs minipig NNS work_infoczas7nfbzac5mhjnxpkxle 72 27 expressing express VBG work_infoczas7nfbzac5mhjnxpkxle 72 28 the the DT work_infoczas7nfbzac5mhjnxpkxle 72 29 green green JJ work_infoczas7nfbzac5mhjnxpkxle 72 30 fluorescent fluorescent NN work_infoczas7nfbzac5mhjnxpkxle 72 31 protein protein NN work_infoczas7nfbzac5mhjnxpkxle 72 32 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 72 33 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 72 34 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 72 35 reporter reporter NN work_infoczas7nfbzac5mhjnxpkxle 72 36 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 72 37 . . . work_infoczas7nfbzac5mhjnxpkxle 73 1 Transposable transposable JJ work_infoczas7nfbzac5mhjnxpkxle 73 2 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 73 3 elements element NNS work_infoczas7nfbzac5mhjnxpkxle 73 4 are be VBP work_infoczas7nfbzac5mhjnxpkxle 73 5 mobile mobile JJ work_infoczas7nfbzac5mhjnxpkxle 73 6 genetic genetic JJ work_infoczas7nfbzac5mhjnxpkxle 73 7 entities entity NNS work_infoczas7nfbzac5mhjnxpkxle 73 8 that that WDT work_infoczas7nfbzac5mhjnxpkxle 73 9 integrate integrate VBP work_infoczas7nfbzac5mhjnxpkxle 73 10 into into IN work_infoczas7nfbzac5mhjnxpkxle 73 11 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 73 12 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 73 13 in in IN work_infoczas7nfbzac5mhjnxpkxle 73 14 a a DT work_infoczas7nfbzac5mhjnxpkxle 73 15 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 73 16 process process NN work_infoczas7nfbzac5mhjnxpkxle 73 17 catalyzed catalyze VBN work_infoczas7nfbzac5mhjnxpkxle 73 18 by by IN work_infoczas7nfbzac5mhjnxpkxle 73 19 the the DT work_infoczas7nfbzac5mhjnxpkxle 73 20 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 73 21 enzyme enzyme NNS work_infoczas7nfbzac5mhjnxpkxle 73 22 encoded encode VBN work_infoczas7nfbzac5mhjnxpkxle 73 23 by by IN work_infoczas7nfbzac5mhjnxpkxle 73 24 the the DT work_infoczas7nfbzac5mhjnxpkxle 73 25 element element NN work_infoczas7nfbzac5mhjnxpkxle 73 26 itself -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 73 27 . . . work_infoczas7nfbzac5mhjnxpkxle 74 1 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 74 2 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 74 3 a a DT work_infoczas7nfbzac5mhjnxpkxle 74 4 cut cut VBN work_infoczas7nfbzac5mhjnxpkxle 74 5 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 74 6 and and CC work_infoczas7nfbzac5mhjnxpkxle 74 7 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 74 8 paste paste NN work_infoczas7nfbzac5mhjnxpkxle 74 9 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 74 10 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 74 11 belonging belong VBG work_infoczas7nfbzac5mhjnxpkxle 74 12 to to IN work_infoczas7nfbzac5mhjnxpkxle 74 13 the the DT work_infoczas7nfbzac5mhjnxpkxle 74 14 Tc1 Tc1 NNP work_infoczas7nfbzac5mhjnxpkxle 74 15 / / SYM work_infoczas7nfbzac5mhjnxpkxle 74 16 mariner mariner NNP work_infoczas7nfbzac5mhjnxpkxle 74 17 superfamily superfamily RB work_infoczas7nfbzac5mhjnxpkxle 74 18 of of IN work_infoczas7nfbzac5mhjnxpkxle 74 19 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 74 20 elements element NNS work_infoczas7nfbzac5mhjnxpkxle 74 21 . . . work_infoczas7nfbzac5mhjnxpkxle 75 1 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 75 2 was be VBD work_infoczas7nfbzac5mhjnxpkxle 75 3 genetically genetically RB work_infoczas7nfbzac5mhjnxpkxle 75 4 reconstructed reconstruct VBN work_infoczas7nfbzac5mhjnxpkxle 75 5 from from IN work_infoczas7nfbzac5mhjnxpkxle 75 6 fossil fossil JJ work_infoczas7nfbzac5mhjnxpkxle 75 7 elements element NNS work_infoczas7nfbzac5mhjnxpkxle 75 8 in in IN work_infoczas7nfbzac5mhjnxpkxle 75 9 the the DT work_infoczas7nfbzac5mhjnxpkxle 75 10 genome genome NN work_infoczas7nfbzac5mhjnxpkxle 75 11 of of IN work_infoczas7nfbzac5mhjnxpkxle 75 12 salmoid salmoid JJ work_infoczas7nfbzac5mhjnxpkxle 75 13 fish fish NN work_infoczas7nfbzac5mhjnxpkxle 75 14 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 75 15 Ivics Ivics NNP work_infoczas7nfbzac5mhjnxpkxle 75 16 et et NNP work_infoczas7nfbzac5mhjnxpkxle 75 17 al al NNP work_infoczas7nfbzac5mhjnxpkxle 75 18 . . . work_infoczas7nfbzac5mhjnxpkxle 76 1 1997 1997 CD work_infoczas7nfbzac5mhjnxpkxle 76 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 76 3 and and CC work_infoczas7nfbzac5mhjnxpkxle 76 4 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 76 5 5 5 CD work_infoczas7nfbzac5mhjnxpkxle 76 6 actively actively RB work_infoczas7nfbzac5mhjnxpkxle 76 7 mobilized mobilize VBN work_infoczas7nfbzac5mhjnxpkxle 76 8 in in IN work_infoczas7nfbzac5mhjnxpkxle 76 9 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 76 10 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 76 11 from from IN work_infoczas7nfbzac5mhjnxpkxle 76 12 a a DT work_infoczas7nfbzac5mhjnxpkxle 76 13 variety variety NN work_infoczas7nfbzac5mhjnxpkxle 76 14 of of IN work_infoczas7nfbzac5mhjnxpkxle 76 15 vertebrate vertebrate NN work_infoczas7nfbzac5mhjnxpkxle 76 16 species specie NNS work_infoczas7nfbzac5mhjnxpkxle 76 17 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 76 18 Izsvak Izsvak NNP work_infoczas7nfbzac5mhjnxpkxle 76 19 et et FW work_infoczas7nfbzac5mhjnxpkxle 76 20 al al NNP work_infoczas7nfbzac5mhjnxpkxle 76 21 . . . work_infoczas7nfbzac5mhjnxpkxle 77 1 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 77 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 77 3 . . . work_infoczas7nfbzac5mhjnxpkxle 78 1 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 78 2 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 78 3 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 78 4 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 78 5 efficiently efficiently RB work_infoczas7nfbzac5mhjnxpkxle 78 6 jump jump VBP work_infoczas7nfbzac5mhjnxpkxle 78 7 from from IN work_infoczas7nfbzac5mhjnxpkxle 78 8 plasmid plasmid JJ work_infoczas7nfbzac5mhjnxpkxle 78 9 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 78 10 introduced introduce VBN work_infoczas7nfbzac5mhjnxpkxle 78 11 into into IN work_infoczas7nfbzac5mhjnxpkxle 78 12 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 78 13 to to IN work_infoczas7nfbzac5mhjnxpkxle 78 14 chromosomal chromosomal NN work_infoczas7nfbzac5mhjnxpkxle 78 15 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 78 16 and and CC work_infoczas7nfbzac5mhjnxpkxle 78 17 are be VBP work_infoczas7nfbzac5mhjnxpkxle 78 18 inserted insert VBN work_infoczas7nfbzac5mhjnxpkxle 78 19 without without IN work_infoczas7nfbzac5mhjnxpkxle 78 20 particular particular JJ work_infoczas7nfbzac5mhjnxpkxle 78 21 preference preference NN work_infoczas7nfbzac5mhjnxpkxle 78 22 for for IN work_infoczas7nfbzac5mhjnxpkxle 78 23 actively actively RB work_infoczas7nfbzac5mhjnxpkxle 78 24 transcribed transcribe VBN work_infoczas7nfbzac5mhjnxpkxle 78 25 genes gene NNS work_infoczas7nfbzac5mhjnxpkxle 78 26 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 78 27 Liu Liu NNP work_infoczas7nfbzac5mhjnxpkxle 78 28 et et NNP work_infoczas7nfbzac5mhjnxpkxle 78 29 al al NNP work_infoczas7nfbzac5mhjnxpkxle 78 30 . . . work_infoczas7nfbzac5mhjnxpkxle 79 1 2005 2005 LS work_infoczas7nfbzac5mhjnxpkxle 79 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 79 3 Yant Yant NNP work_infoczas7nfbzac5mhjnxpkxle 79 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 79 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 79 6 . . . work_infoczas7nfbzac5mhjnxpkxle 80 1 2005 2005 CD work_infoczas7nfbzac5mhjnxpkxle 80 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 80 3 . . . work_infoczas7nfbzac5mhjnxpkxle 81 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 81 2 element element NN work_infoczas7nfbzac5mhjnxpkxle 81 3 has have VBZ work_infoczas7nfbzac5mhjnxpkxle 81 4 been be VBN work_infoczas7nfbzac5mhjnxpkxle 81 5 explored explore VBN work_infoczas7nfbzac5mhjnxpkxle 81 6 for for IN work_infoczas7nfbzac5mhjnxpkxle 81 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 81 8 use use NN work_infoczas7nfbzac5mhjnxpkxle 81 9 in in IN work_infoczas7nfbzac5mhjnxpkxle 81 10 therapeutic therapeutic JJ work_infoczas7nfbzac5mhjnxpkxle 81 11 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 81 12 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 81 13 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 81 14 Izsvak Izsvak NNP work_infoczas7nfbzac5mhjnxpkxle 81 15 and and CC work_infoczas7nfbzac5mhjnxpkxle 81 16 Ivics Ivics NNP work_infoczas7nfbzac5mhjnxpkxle 81 17 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 81 18 ; ; : work_infoczas7nfbzac5mhjnxpkxle 81 19 Liu Liu NNP work_infoczas7nfbzac5mhjnxpkxle 81 20 et et NNP work_infoczas7nfbzac5mhjnxpkxle 81 21 al al NNP work_infoczas7nfbzac5mhjnxpkxle 81 22 . . . work_infoczas7nfbzac5mhjnxpkxle 82 1 2006 2006 CD work_infoczas7nfbzac5mhjnxpkxle 82 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 82 3 Mates Mates NNP work_infoczas7nfbzac5mhjnxpkxle 82 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 82 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 82 6 . . . work_infoczas7nfbzac5mhjnxpkxle 83 1 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 83 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 83 3 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 83 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 83 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 83 6 . . . work_infoczas7nfbzac5mhjnxpkxle 84 1 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 84 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 84 3 Ohlfest Ohlfest NNP work_infoczas7nfbzac5mhjnxpkxle 84 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 84 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 84 6 . . . work_infoczas7nfbzac5mhjnxpkxle 85 1 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 85 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 85 3 Singh Singh NNP work_infoczas7nfbzac5mhjnxpkxle 85 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 85 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 85 6 . . . work_infoczas7nfbzac5mhjnxpkxle 86 1 2008 2008 LS work_infoczas7nfbzac5mhjnxpkxle 86 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 86 3 Yant Yant NNP work_infoczas7nfbzac5mhjnxpkxle 86 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 86 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 86 6 . . . work_infoczas7nfbzac5mhjnxpkxle 87 1 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 87 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 87 3 and and CC work_infoczas7nfbzac5mhjnxpkxle 87 4 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 87 5 widely widely RB work_infoczas7nfbzac5mhjnxpkxle 87 6 used use VBN work_infoczas7nfbzac5mhjnxpkxle 87 7 as as IN work_infoczas7nfbzac5mhjnxpkxle 87 8 a a DT work_infoczas7nfbzac5mhjnxpkxle 87 9 tool tool NN work_infoczas7nfbzac5mhjnxpkxle 87 10 in in IN work_infoczas7nfbzac5mhjnxpkxle 87 11 animal animal NN work_infoczas7nfbzac5mhjnxpkxle 87 12 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 87 13 . . . work_infoczas7nfbzac5mhjnxpkxle 88 1 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 88 2 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 88 3 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 88 4 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 88 5 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 88 6 well well RB work_infoczas7nfbzac5mhjnxpkxle 88 7 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 88 8 established establish VBN work_infoczas7nfbzac5mhjnxpkxle 88 9 in in IN work_infoczas7nfbzac5mhjnxpkxle 88 10 zebrafish zebrafish NNP work_infoczas7nfbzac5mhjnxpkxle 88 11 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 88 12 Davidson Davidson NNP work_infoczas7nfbzac5mhjnxpkxle 88 13 et et NNP work_infoczas7nfbzac5mhjnxpkxle 88 14 al al NNP work_infoczas7nfbzac5mhjnxpkxle 88 15 . . . work_infoczas7nfbzac5mhjnxpkxle 89 1 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 89 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 89 3 , , , work_infoczas7nfbzac5mhjnxpkxle 89 4 and and CC work_infoczas7nfbzac5mhjnxpkxle 89 5 germline germline VB work_infoczas7nfbzac5mhjnxpkxle 89 6 mutagenesis mutagenesis NN work_infoczas7nfbzac5mhjnxpkxle 89 7 by by IN work_infoczas7nfbzac5mhjnxpkxle 89 8 microinjection microinjection NN work_infoczas7nfbzac5mhjnxpkxle 89 9 of of IN work_infoczas7nfbzac5mhjnxpkxle 89 10 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 89 11 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 89 12 and and CC work_infoczas7nfbzac5mhjnxpkxle 89 13 transposase- transposase- NN work_infoczas7nfbzac5mhjnxpkxle 89 14 encoding encode VBG work_infoczas7nfbzac5mhjnxpkxle 89 15 nucleic nucleic NN work_infoczas7nfbzac5mhjnxpkxle 89 16 acids acid NNS work_infoczas7nfbzac5mhjnxpkxle 89 17 into into IN work_infoczas7nfbzac5mhjnxpkxle 89 18 fertilized fertilize VBN work_infoczas7nfbzac5mhjnxpkxle 89 19 oocytes oocyte NNS work_infoczas7nfbzac5mhjnxpkxle 89 20 has have VBZ work_infoczas7nfbzac5mhjnxpkxle 89 21 been be VBN work_infoczas7nfbzac5mhjnxpkxle 89 22 established establish VBN work_infoczas7nfbzac5mhjnxpkxle 89 23 in in IN work_infoczas7nfbzac5mhjnxpkxle 89 24 the the DT work_infoczas7nfbzac5mhjnxpkxle 89 25 mouse mouse NN work_infoczas7nfbzac5mhjnxpkxle 89 26 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 89 27 Dupuy Dupuy NNP work_infoczas7nfbzac5mhjnxpkxle 89 28 et et NNP work_infoczas7nfbzac5mhjnxpkxle 89 29 al al NNP work_infoczas7nfbzac5mhjnxpkxle 89 30 . . . work_infoczas7nfbzac5mhjnxpkxle 90 1 2002 2002 CD work_infoczas7nfbzac5mhjnxpkxle 90 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 90 3 Mates Mates NNP work_infoczas7nfbzac5mhjnxpkxle 90 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 90 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 90 6 . . . work_infoczas7nfbzac5mhjnxpkxle 91 1 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 91 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 91 3 and and CC work_infoczas7nfbzac5mhjnxpkxle 91 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 91 5 frog frog NN work_infoczas7nfbzac5mhjnxpkxle 91 6 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 91 7 Sinzelle Sinzelle NNP work_infoczas7nfbzac5mhjnxpkxle 91 8 et et FW work_infoczas7nfbzac5mhjnxpkxle 91 9 al al NNP work_infoczas7nfbzac5mhjnxpkxle 91 10 . . . work_infoczas7nfbzac5mhjnxpkxle 92 1 2006 2006 CD work_infoczas7nfbzac5mhjnxpkxle 92 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 92 3 Yergeau Yergeau NNP work_infoczas7nfbzac5mhjnxpkxle 92 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 92 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 92 6 . . . work_infoczas7nfbzac5mhjnxpkxle 93 1 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 93 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 93 3 . . . work_infoczas7nfbzac5mhjnxpkxle 94 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 94 2 properties property NNS work_infoczas7nfbzac5mhjnxpkxle 94 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 94 4 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 94 5 as as IN work_infoczas7nfbzac5mhjnxpkxle 94 6 an an DT work_infoczas7nfbzac5mhjnxpkxle 94 7 efficient efficient JJ work_infoczas7nfbzac5mhjnxpkxle 94 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 94 9 genetically genetically RB work_infoczas7nfbzac5mhjnxpkxle 94 10 simple simple JJ work_infoczas7nfbzac5mhjnxpkxle 94 11 non non JJ work_infoczas7nfbzac5mhjnxpkxle 94 12 - - JJ work_infoczas7nfbzac5mhjnxpkxle 94 13 viral viral JJ work_infoczas7nfbzac5mhjnxpkxle 94 14 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 94 15 delivery delivery NN work_infoczas7nfbzac5mhjnxpkxle 94 16 system system NN work_infoczas7nfbzac5mhjnxpkxle 94 17 facilitating facilitate VBG work_infoczas7nfbzac5mhjnxpkxle 94 18 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 94 19 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 94 20 with with IN work_infoczas7nfbzac5mhjnxpkxle 94 21 a a DT work_infoczas7nfbzac5mhjnxpkxle 94 22 random random JJ work_infoczas7nfbzac5mhjnxpkxle 94 23 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 94 24 profile profile NN work_infoczas7nfbzac5mhjnxpkxle 94 25 make make VB work_infoczas7nfbzac5mhjnxpkxle 94 26 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 94 27 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 94 28 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 94 29 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 94 30 relevant relevant JJ work_infoczas7nfbzac5mhjnxpkxle 94 31 tools tool NNS work_infoczas7nfbzac5mhjnxpkxle 94 32 for for IN work_infoczas7nfbzac5mhjnxpkxle 94 33 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 94 34 also also RB work_infoczas7nfbzac5mhjnxpkxle 94 35 of of IN work_infoczas7nfbzac5mhjnxpkxle 94 36 larger large JJR work_infoczas7nfbzac5mhjnxpkxle 94 37 animals animal NNS work_infoczas7nfbzac5mhjnxpkxle 94 38 . . . work_infoczas7nfbzac5mhjnxpkxle 95 1 A a DT work_infoczas7nfbzac5mhjnxpkxle 95 2 routine routine JJ work_infoczas7nfbzac5mhjnxpkxle 95 3 use use NN work_infoczas7nfbzac5mhjnxpkxle 95 4 of of IN work_infoczas7nfbzac5mhjnxpkxle 95 5 transposons transposon NNS work_infoczas7nfbzac5mhjnxpkxle 95 6 may may MD work_infoczas7nfbzac5mhjnxpkxle 95 7 be be VB work_infoczas7nfbzac5mhjnxpkxle 95 8 expected expect VBN work_infoczas7nfbzac5mhjnxpkxle 95 9 to to TO work_infoczas7nfbzac5mhjnxpkxle 95 10 improve improve VB work_infoczas7nfbzac5mhjnxpkxle 95 11 the the DT work_infoczas7nfbzac5mhjnxpkxle 95 12 efficiency efficiency NN work_infoczas7nfbzac5mhjnxpkxle 95 13 of of IN work_infoczas7nfbzac5mhjnxpkxle 95 14 cellular cellular JJ work_infoczas7nfbzac5mhjnxpkxle 95 15 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 95 16 and and CC work_infoczas7nfbzac5mhjnxpkxle 95 17 to to TO work_infoczas7nfbzac5mhjnxpkxle 95 18 reduce reduce VB work_infoczas7nfbzac5mhjnxpkxle 95 19 concatamer concatamer NN work_infoczas7nfbzac5mhjnxpkxle 95 20 formation formation NN work_infoczas7nfbzac5mhjnxpkxle 95 21 and and CC work_infoczas7nfbzac5mhjnxpkxle 95 22 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 95 23 of of IN work_infoczas7nfbzac5mhjnxpkxle 95 24 CpG CpG NNP work_infoczas7nfbzac5mhjnxpkxle 95 25 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 95 26 rich rich JJ work_infoczas7nfbzac5mhjnxpkxle 95 27 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 95 28 sequence sequence NN work_infoczas7nfbzac5mhjnxpkxle 95 29 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 95 30 Clark Clark NNP work_infoczas7nfbzac5mhjnxpkxle 95 31 et et NNP work_infoczas7nfbzac5mhjnxpkxle 95 32 al al NNP work_infoczas7nfbzac5mhjnxpkxle 95 33 . . . work_infoczas7nfbzac5mhjnxpkxle 96 1 2007a 2007a LS work_infoczas7nfbzac5mhjnxpkxle 96 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 96 3 . . . work_infoczas7nfbzac5mhjnxpkxle 97 1 By by IN work_infoczas7nfbzac5mhjnxpkxle 97 2 transfecting transfecte VBG work_infoczas7nfbzac5mhjnxpkxle 97 3 skin skin NN work_infoczas7nfbzac5mhjnxpkxle 97 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 97 5 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 97 6 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 97 7 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 97 8 with with IN work_infoczas7nfbzac5mhjnxpkxle 97 9 plasmid plasmid NNP work_infoczas7nfbzac5mhjnxpkxle 97 10 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 97 11 carrying carry VBG work_infoczas7nfbzac5mhjnxpkxle 97 12 the the DT work_infoczas7nfbzac5mhjnxpkxle 97 13 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 97 14 reporter reporter NN work_infoczas7nfbzac5mhjnxpkxle 97 15 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 97 16 , , , work_infoczas7nfbzac5mhjnxpkxle 97 17 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 97 18 previously previously RB work_infoczas7nfbzac5mhjnxpkxle 97 19 reported report VBD work_infoczas7nfbzac5mhjnxpkxle 97 20 production production NN work_infoczas7nfbzac5mhjnxpkxle 97 21 of of IN work_infoczas7nfbzac5mhjnxpkxle 97 22 blastocysts blastocyst NNS work_infoczas7nfbzac5mhjnxpkxle 97 23 harboring harbor VBG work_infoczas7nfbzac5mhjnxpkxle 97 24 randomly randomly RB work_infoczas7nfbzac5mhjnxpkxle 97 25 inserted insert VBN work_infoczas7nfbzac5mhjnxpkxle 97 26 plasmid plasmid NNP work_infoczas7nfbzac5mhjnxpkxle 97 27 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 97 28 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 97 29 Kragh Kragh NNP work_infoczas7nfbzac5mhjnxpkxle 97 30 et et FW work_infoczas7nfbzac5mhjnxpkxle 97 31 al al NNP work_infoczas7nfbzac5mhjnxpkxle 97 32 . . . work_infoczas7nfbzac5mhjnxpkxle 98 1 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 98 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 98 3 . . . work_infoczas7nfbzac5mhjnxpkxle 99 1 Active active JJ work_infoczas7nfbzac5mhjnxpkxle 99 2 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 99 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 99 4 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 99 5 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 99 6 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 99 7 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 99 8 in in IN work_infoczas7nfbzac5mhjnxpkxle 99 9 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 99 10 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 99 11 has have VBZ work_infoczas7nfbzac5mhjnxpkxle 99 12 recently recently RB work_infoczas7nfbzac5mhjnxpkxle 99 13 been be VBN work_infoczas7nfbzac5mhjnxpkxle 99 14 documented document VBN work_infoczas7nfbzac5mhjnxpkxle 99 15 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 99 16 Clark Clark NNP work_infoczas7nfbzac5mhjnxpkxle 99 17 et et NNP work_infoczas7nfbzac5mhjnxpkxle 99 18 al al NNP work_infoczas7nfbzac5mhjnxpkxle 99 19 . . . work_infoczas7nfbzac5mhjnxpkxle 100 1 2007b 2007b LS work_infoczas7nfbzac5mhjnxpkxle 100 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 100 3 . . . work_infoczas7nfbzac5mhjnxpkxle 101 1 Using use VBG work_infoczas7nfbzac5mhjnxpkxle 101 2 the the DT work_infoczas7nfbzac5mhjnxpkxle 101 3 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 101 4 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 101 5 as as IN work_infoczas7nfbzac5mhjnxpkxle 101 6 a a DT work_infoczas7nfbzac5mhjnxpkxle 101 7 reporter reporter NN work_infoczas7nfbzac5mhjnxpkxle 101 8 , , , work_infoczas7nfbzac5mhjnxpkxle 101 9 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 101 10 here here RB work_infoczas7nfbzac5mhjnxpkxle 101 11 combine combine VBP work_infoczas7nfbzac5mhjnxpkxle 101 12 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 101 13 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 101 14 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 101 15 engineering engineering NN work_infoczas7nfbzac5mhjnxpkxle 101 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 101 17 neonatal neonatal JJ work_infoczas7nfbzac5mhjnxpkxle 101 18 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 101 19 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 101 20 with with IN work_infoczas7nfbzac5mhjnxpkxle 101 21 HMC HMC NNP work_infoczas7nfbzac5mhjnxpkxle 101 22 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 101 23 based base VBN work_infoczas7nfbzac5mhjnxpkxle 101 24 SCNT scnt NN work_infoczas7nfbzac5mhjnxpkxle 101 25 and and CC work_infoczas7nfbzac5mhjnxpkxle 101 26 production production NN work_infoczas7nfbzac5mhjnxpkxle 101 27 of of IN work_infoczas7nfbzac5mhjnxpkxle 101 28 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 101 29 cloned clone VBN work_infoczas7nfbzac5mhjnxpkxle 101 30 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 101 31 . . . work_infoczas7nfbzac5mhjnxpkxle 102 1 We -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 102 2 describe describe VBP work_infoczas7nfbzac5mhjnxpkxle 102 3 events event NNS work_infoczas7nfbzac5mhjnxpkxle 102 4 of of IN work_infoczas7nfbzac5mhjnxpkxle 102 5 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 102 6 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 102 7 leading lead VBG work_infoczas7nfbzac5mhjnxpkxle 102 8 to to IN work_infoczas7nfbzac5mhjnxpkxle 102 9 the the DT work_infoczas7nfbzac5mhjnxpkxle 102 10 production production NN work_infoczas7nfbzac5mhjnxpkxle 102 11 of of IN work_infoczas7nfbzac5mhjnxpkxle 102 12 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 102 13 cloned clone VBN work_infoczas7nfbzac5mhjnxpkxle 102 14 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 102 15 with with IN work_infoczas7nfbzac5mhjnxpkxle 102 16 uniform uniform JJ work_infoczas7nfbzac5mhjnxpkxle 102 17 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 102 18 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 102 19 in in IN work_infoczas7nfbzac5mhjnxpkxle 102 20 all all DT work_infoczas7nfbzac5mhjnxpkxle 102 21 analyzed analyze VBN work_infoczas7nfbzac5mhjnxpkxle 102 22 organs organ NNS work_infoczas7nfbzac5mhjnxpkxle 102 23 , , , work_infoczas7nfbzac5mhjnxpkxle 102 24 but but CC work_infoczas7nfbzac5mhjnxpkxle 102 25 also also RB work_infoczas7nfbzac5mhjnxpkxle 102 26 describe describe VB work_infoczas7nfbzac5mhjnxpkxle 102 27 potential potential JJ work_infoczas7nfbzac5mhjnxpkxle 102 28 critical critical JJ work_infoczas7nfbzac5mhjnxpkxle 102 29 issues issue NNS work_infoczas7nfbzac5mhjnxpkxle 102 30 on on IN work_infoczas7nfbzac5mhjnxpkxle 102 31 the the DT work_infoczas7nfbzac5mhjnxpkxle 102 32 path path NN work_infoczas7nfbzac5mhjnxpkxle 102 33 towards towards IN work_infoczas7nfbzac5mhjnxpkxle 102 34 creating create VBG work_infoczas7nfbzac5mhjnxpkxle 102 35 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 102 36 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 102 37 models model NNS work_infoczas7nfbzac5mhjnxpkxle 102 38 using use VBG work_infoczas7nfbzac5mhjnxpkxle 102 39 the the DT work_infoczas7nfbzac5mhjnxpkxle 102 40 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 102 41 technology technology NN work_infoczas7nfbzac5mhjnxpkxle 102 42 . . . work_infoczas7nfbzac5mhjnxpkxle 103 1 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 103 2 RESULTS results NN work_infoczas7nfbzac5mhjnxpkxle 103 3 Generation generation NN work_infoczas7nfbzac5mhjnxpkxle 103 4 of of IN work_infoczas7nfbzac5mhjnxpkxle 103 5 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 103 6 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 103 7 by by IN work_infoczas7nfbzac5mhjnxpkxle 103 8 SCNT SCNT NNP work_infoczas7nfbzac5mhjnxpkxle 103 9 using use VBG work_infoczas7nfbzac5mhjnxpkxle 103 10 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 103 11 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 103 12 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 103 13 modified modify VBN work_infoczas7nfbzac5mhjnxpkxle 103 14 neonatal neonatal JJ work_infoczas7nfbzac5mhjnxpkxle 103 15 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 103 16 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 103 17 Production production NN work_infoczas7nfbzac5mhjnxpkxle 103 18 of of IN work_infoczas7nfbzac5mhjnxpkxle 103 19 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 103 20 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 103 21 by by IN work_infoczas7nfbzac5mhjnxpkxle 103 22 SCNT SCNT NNP work_infoczas7nfbzac5mhjnxpkxle 103 23 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 103 24 based base VBN work_infoczas7nfbzac5mhjnxpkxle 103 25 on on IN work_infoczas7nfbzac5mhjnxpkxle 103 26 the the DT work_infoczas7nfbzac5mhjnxpkxle 103 27 genetic genetic JJ work_infoczas7nfbzac5mhjnxpkxle 103 28 engineering engineering NN work_infoczas7nfbzac5mhjnxpkxle 103 29 of of IN work_infoczas7nfbzac5mhjnxpkxle 103 30 primary primary JJ work_infoczas7nfbzac5mhjnxpkxle 103 31 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 103 32 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 103 33 prior prior RB work_infoczas7nfbzac5mhjnxpkxle 103 34 to to IN work_infoczas7nfbzac5mhjnxpkxle 103 35 nuclear nuclear JJ work_infoczas7nfbzac5mhjnxpkxle 103 36 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 103 37 . . . work_infoczas7nfbzac5mhjnxpkxle 104 1 To to TO work_infoczas7nfbzac5mhjnxpkxle 104 2 investigate investigate VB work_infoczas7nfbzac5mhjnxpkxle 104 3 the the DT work_infoczas7nfbzac5mhjnxpkxle 104 4 potential potential JJ work_infoczas7nfbzac5mhjnxpkxle 104 5 use use NN work_infoczas7nfbzac5mhjnxpkxle 104 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 104 7 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 104 8 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 104 9 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 104 10 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 104 11 in in IN work_infoczas7nfbzac5mhjnxpkxle 104 12 minipig minipig NNP work_infoczas7nfbzac5mhjnxpkxle 104 13 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 104 14 , , , work_infoczas7nfbzac5mhjnxpkxle 104 15 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 104 16 constructed construct VBD work_infoczas7nfbzac5mhjnxpkxle 104 17 a a DT work_infoczas7nfbzac5mhjnxpkxle 104 18 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 104 19 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 104 20 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 104 21 , , , work_infoczas7nfbzac5mhjnxpkxle 104 22 designated designate VBD work_infoczas7nfbzac5mhjnxpkxle 104 23 pSBT psbt JJ work_infoczas7nfbzac5mhjnxpkxle 104 24 / / SYM work_infoczas7nfbzac5mhjnxpkxle 104 25 cHS4.Ubi cHS4.Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 104 26 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 104 27 GIN.cHS4 GIN.cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 104 28 , , , work_infoczas7nfbzac5mhjnxpkxle 104 29 harboring harbor VBG work_infoczas7nfbzac5mhjnxpkxle 104 30 a a DT work_infoczas7nfbzac5mhjnxpkxle 104 31 bicistronic bicistronic JJ work_infoczas7nfbzac5mhjnxpkxle 104 32 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 104 33 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 104 34 with with IN work_infoczas7nfbzac5mhjnxpkxle 104 35 a a DT work_infoczas7nfbzac5mhjnxpkxle 104 36 reporter reporter NN work_infoczas7nfbzac5mhjnxpkxle 104 37 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 104 38 encoding encode VBG work_infoczas7nfbzac5mhjnxpkxle 104 39 green green JJ work_infoczas7nfbzac5mhjnxpkxle 104 40 fluorescent fluorescent NN work_infoczas7nfbzac5mhjnxpkxle 104 41 protein protein NN work_infoczas7nfbzac5mhjnxpkxle 104 42 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 104 43 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 104 44 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 104 45 linked link VBD work_infoczas7nfbzac5mhjnxpkxle 104 46 to to IN work_infoczas7nfbzac5mhjnxpkxle 104 47 a a DT work_infoczas7nfbzac5mhjnxpkxle 104 48 neomycin neomycin NNP work_infoczas7nfbzac5mhjnxpkxle 104 49 resistance resistance NNP work_infoczas7nfbzac5mhjnxpkxle 104 50 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 104 51 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 104 52 neo neo NNP work_infoczas7nfbzac5mhjnxpkxle 104 53 r r NNP work_infoczas7nfbzac5mhjnxpkxle 104 54 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 104 55 through through IN work_infoczas7nfbzac5mhjnxpkxle 104 56 an an DT work_infoczas7nfbzac5mhjnxpkxle 104 57 internal internal JJ work_infoczas7nfbzac5mhjnxpkxle 104 58 ribosomal ribosomal NN work_infoczas7nfbzac5mhjnxpkxle 104 59 entry entry NN work_infoczas7nfbzac5mhjnxpkxle 104 60 site site NN work_infoczas7nfbzac5mhjnxpkxle 104 61 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 104 62 IRES ires NN work_infoczas7nfbzac5mhjnxpkxle 104 63 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 104 64 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 104 65 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 104 66 . . . work_infoczas7nfbzac5mhjnxpkxle 105 1 1A 1a LS work_infoczas7nfbzac5mhjnxpkxle 105 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 105 3 . . . work_infoczas7nfbzac5mhjnxpkxle 106 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 106 2 human human JJ work_infoczas7nfbzac5mhjnxpkxle 106 3 ubiquitin ubiquitin JJ work_infoczas7nfbzac5mhjnxpkxle 106 4 C c NN work_infoczas7nfbzac5mhjnxpkxle 106 5 promoter promoter NN work_infoczas7nfbzac5mhjnxpkxle 106 6 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 106 7 Ubi ubi NN work_infoczas7nfbzac5mhjnxpkxle 106 8 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 106 9 was be VBD work_infoczas7nfbzac5mhjnxpkxle 106 10 utilized utilize VBN work_infoczas7nfbzac5mhjnxpkxle 106 11 to to TO work_infoczas7nfbzac5mhjnxpkxle 106 12 drive drive VB work_infoczas7nfbzac5mhjnxpkxle 106 13 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 106 14 , , , work_infoczas7nfbzac5mhjnxpkxle 106 15 and and CC work_infoczas7nfbzac5mhjnxpkxle 106 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 106 17 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 106 18 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 106 19 was be VBD work_infoczas7nfbzac5mhjnxpkxle 106 20 flanked flank VBN work_infoczas7nfbzac5mhjnxpkxle 106 21 on on IN work_infoczas7nfbzac5mhjnxpkxle 106 22 each each DT work_infoczas7nfbzac5mhjnxpkxle 106 23 side side NN work_infoczas7nfbzac5mhjnxpkxle 106 24 by by IN work_infoczas7nfbzac5mhjnxpkxle 106 25 1.2-kb 1.2-kb CD work_infoczas7nfbzac5mhjnxpkxle 106 26 long long JJ work_infoczas7nfbzac5mhjnxpkxle 106 27 5’-HS4 5’-HS4 NNP work_infoczas7nfbzac5mhjnxpkxle 106 28 chicken chicken NN work_infoczas7nfbzac5mhjnxpkxle 106 29 β β NNP work_infoczas7nfbzac5mhjnxpkxle 106 30 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 106 31 globin globin NNP work_infoczas7nfbzac5mhjnxpkxle 106 32 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 106 33 cHS4 cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 106 34 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 106 35 insulators insulator NNS work_infoczas7nfbzac5mhjnxpkxle 106 36 , , , work_infoczas7nfbzac5mhjnxpkxle 106 37 which which WDT work_infoczas7nfbzac5mhjnxpkxle 106 38 have have VBP work_infoczas7nfbzac5mhjnxpkxle 106 39 been be VBN work_infoczas7nfbzac5mhjnxpkxle 106 40 shown show VBN work_infoczas7nfbzac5mhjnxpkxle 106 41 to to TO work_infoczas7nfbzac5mhjnxpkxle 106 42 protect protect VB work_infoczas7nfbzac5mhjnxpkxle 106 43 the the DT work_infoczas7nfbzac5mhjnxpkxle 106 44 genetic genetic JJ work_infoczas7nfbzac5mhjnxpkxle 106 45 cargo cargo NN work_infoczas7nfbzac5mhjnxpkxle 106 46 of of IN work_infoczas7nfbzac5mhjnxpkxle 106 47 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 106 48 transposons transposon NNS work_infoczas7nfbzac5mhjnxpkxle 106 49 from from IN work_infoczas7nfbzac5mhjnxpkxle 106 50 transcriptional transcriptional JJ work_infoczas7nfbzac5mhjnxpkxle 106 51 silencing silencing NN work_infoczas7nfbzac5mhjnxpkxle 106 52 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 106 53 Dalsgaard Dalsgaard NNP work_infoczas7nfbzac5mhjnxpkxle 106 54 et et FW work_infoczas7nfbzac5mhjnxpkxle 106 55 al al NNP work_infoczas7nfbzac5mhjnxpkxle 106 56 . . . work_infoczas7nfbzac5mhjnxpkxle 107 1 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 107 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 107 3 . . . work_infoczas7nfbzac5mhjnxpkxle 108 1 Transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 108 2 in in IN work_infoczas7nfbzac5mhjnxpkxle 108 3 neonatal neonatal JJ work_infoczas7nfbzac5mhjnxpkxle 108 4 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 108 5 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 108 6 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 108 7 NPFs NPFs NNP work_infoczas7nfbzac5mhjnxpkxle 108 8 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 108 9 was be VBD work_infoczas7nfbzac5mhjnxpkxle 108 10 carried carry VBN work_infoczas7nfbzac5mhjnxpkxle 108 11 out out RP work_infoczas7nfbzac5mhjnxpkxle 108 12 by by IN work_infoczas7nfbzac5mhjnxpkxle 108 13 co co NN work_infoczas7nfbzac5mhjnxpkxle 108 14 - - JJ work_infoczas7nfbzac5mhjnxpkxle 108 15 transfecting transfecting JJ work_infoczas7nfbzac5mhjnxpkxle 108 16 pSBT psbt NN work_infoczas7nfbzac5mhjnxpkxle 108 17 / / SYM work_infoczas7nfbzac5mhjnxpkxle 108 18 cHS4.Ubi cHS4.Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 108 19 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 108 20 GIN.cHS4 GIN.cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 108 21 with with IN work_infoczas7nfbzac5mhjnxpkxle 108 22 an an DT work_infoczas7nfbzac5mhjnxpkxle 108 23 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 108 24 construct construct NN work_infoczas7nfbzac5mhjnxpkxle 108 25 , , , work_infoczas7nfbzac5mhjnxpkxle 108 26 pCMV pCMV NNP work_infoczas7nfbzac5mhjnxpkxle 108 27 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 108 28 HSB3 HSB3 NNP work_infoczas7nfbzac5mhjnxpkxle 108 29 , , , work_infoczas7nfbzac5mhjnxpkxle 108 30 encoding encode VBG work_infoczas7nfbzac5mhjnxpkxle 108 31 the the DT work_infoczas7nfbzac5mhjnxpkxle 108 32 hyperactive hyperactive JJ work_infoczas7nfbzac5mhjnxpkxle 108 33 HSB3 HSB3 NNP work_infoczas7nfbzac5mhjnxpkxle 108 34 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 108 35 variant variant JJ work_infoczas7nfbzac5mhjnxpkxle 108 36 . . . work_infoczas7nfbzac5mhjnxpkxle 109 1 This this DT work_infoczas7nfbzac5mhjnxpkxle 109 2 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 109 3 variant variant NN work_infoczas7nfbzac5mhjnxpkxle 109 4 has have VBZ work_infoczas7nfbzac5mhjnxpkxle 109 5 previously previously RB work_infoczas7nfbzac5mhjnxpkxle 109 6 been be VBN work_infoczas7nfbzac5mhjnxpkxle 109 7 shown show VBN work_infoczas7nfbzac5mhjnxpkxle 109 8 to to TO work_infoczas7nfbzac5mhjnxpkxle 109 9 increase increase VB work_infoczas7nfbzac5mhjnxpkxle 109 10 levels level NNS work_infoczas7nfbzac5mhjnxpkxle 109 11 of of IN work_infoczas7nfbzac5mhjnxpkxle 109 12 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 109 13 more more JJR work_infoczas7nfbzac5mhjnxpkxle 109 14 than than IN work_infoczas7nfbzac5mhjnxpkxle 109 15 5-fold 5-fold CD work_infoczas7nfbzac5mhjnxpkxle 109 16 compared compare VBN work_infoczas7nfbzac5mhjnxpkxle 109 17 to to IN work_infoczas7nfbzac5mhjnxpkxle 109 18 the the DT work_infoczas7nfbzac5mhjnxpkxle 109 19 original original JJ work_infoczas7nfbzac5mhjnxpkxle 109 20 SB10 SB10 NNP work_infoczas7nfbzac5mhjnxpkxle 109 21 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 109 22 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 109 23 Yant Yant NNP work_infoczas7nfbzac5mhjnxpkxle 109 24 et et FW work_infoczas7nfbzac5mhjnxpkxle 109 25 al al NNP work_infoczas7nfbzac5mhjnxpkxle 109 26 . . . work_infoczas7nfbzac5mhjnxpkxle 110 1 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 110 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 110 3 . . . work_infoczas7nfbzac5mhjnxpkxle 111 1 By by IN work_infoczas7nfbzac5mhjnxpkxle 111 2 transfecting transfecte VBG work_infoczas7nfbzac5mhjnxpkxle 111 3 supercoiled supercoile VBD work_infoczas7nfbzac5mhjnxpkxle 111 4 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 111 5 donor donor NN work_infoczas7nfbzac5mhjnxpkxle 111 6 plasmid plasmid NNP work_infoczas7nfbzac5mhjnxpkxle 111 7 and and CC work_infoczas7nfbzac5mhjnxpkxle 111 8 HSB3-encoding HSB3-encoding NNP work_infoczas7nfbzac5mhjnxpkxle 111 9 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 111 10 in in IN work_infoczas7nfbzac5mhjnxpkxle 111 11 a a DT work_infoczas7nfbzac5mhjnxpkxle 111 12 40:1 40:1 CD work_infoczas7nfbzac5mhjnxpkxle 111 13 ratio ratio NN work_infoczas7nfbzac5mhjnxpkxle 111 14 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 111 15 using use VBG work_infoczas7nfbzac5mhjnxpkxle 111 16 50 50 CD work_infoczas7nfbzac5mhjnxpkxle 111 17 ng ng NNS work_infoczas7nfbzac5mhjnxpkxle 111 18 of of IN work_infoczas7nfbzac5mhjnxpkxle 111 19 pCMV pCMV NNP work_infoczas7nfbzac5mhjnxpkxle 111 20 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 111 21 HSB3 HSB3 NNP work_infoczas7nfbzac5mhjnxpkxle 111 22 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 111 23 , , , work_infoczas7nfbzac5mhjnxpkxle 111 24 the the DT work_infoczas7nfbzac5mhjnxpkxle 111 25 number number NN work_infoczas7nfbzac5mhjnxpkxle 111 26 of of IN work_infoczas7nfbzac5mhjnxpkxle 111 27 G418-resistant g418-resistant JJ work_infoczas7nfbzac5mhjnxpkxle 111 28 NPF NPF NNP work_infoczas7nfbzac5mhjnxpkxle 111 29 colonies colony NNS work_infoczas7nfbzac5mhjnxpkxle 111 30 was be VBD work_infoczas7nfbzac5mhjnxpkxle 111 31 increased increase VBN work_infoczas7nfbzac5mhjnxpkxle 111 32 12-fold 12-fold CD work_infoczas7nfbzac5mhjnxpkxle 111 33 relative relative NN work_infoczas7nfbzac5mhjnxpkxle 111 34 to to IN work_infoczas7nfbzac5mhjnxpkxle 111 35 an an DT work_infoczas7nfbzac5mhjnxpkxle 111 36 experiment experiment NN work_infoczas7nfbzac5mhjnxpkxle 111 37 in in IN work_infoczas7nfbzac5mhjnxpkxle 111 38 which which WDT work_infoczas7nfbzac5mhjnxpkxle 111 39 HSB3-expressing HSB3-expressing NNP work_infoczas7nfbzac5mhjnxpkxle 111 40 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 111 41 was be VBD work_infoczas7nfbzac5mhjnxpkxle 111 42 replaced replace VBN work_infoczas7nfbzac5mhjnxpkxle 111 43 by by IN work_infoczas7nfbzac5mhjnxpkxle 111 44 the the DT work_infoczas7nfbzac5mhjnxpkxle 111 45 pUC19 puc19 JJ work_infoczas7nfbzac5mhjnxpkxle 111 46 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 111 47 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 111 48 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 111 49 . . . work_infoczas7nfbzac5mhjnxpkxle 112 1 1B 1B NNP work_infoczas7nfbzac5mhjnxpkxle 112 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 112 3 . . . work_infoczas7nfbzac5mhjnxpkxle 113 1 This this DT work_infoczas7nfbzac5mhjnxpkxle 113 2 suggested suggest VBD work_infoczas7nfbzac5mhjnxpkxle 113 3 that that IN work_infoczas7nfbzac5mhjnxpkxle 113 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 113 5 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 113 6 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 113 7 was be VBD work_infoczas7nfbzac5mhjnxpkxle 113 8 actively actively RB work_infoczas7nfbzac5mhjnxpkxle 113 9 mobilized mobilize VBN work_infoczas7nfbzac5mhjnxpkxle 113 10 by by IN work_infoczas7nfbzac5mhjnxpkxle 113 11 the the DT work_infoczas7nfbzac5mhjnxpkxle 113 12 HSB3 HSB3 NNP work_infoczas7nfbzac5mhjnxpkxle 113 13 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 113 14 from from IN work_infoczas7nfbzac5mhjnxpkxle 113 15 transfected transfecte VBN work_infoczas7nfbzac5mhjnxpkxle 113 16 plasmid plasmid NNP work_infoczas7nfbzac5mhjnxpkxle 113 17 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 113 18 and and CC work_infoczas7nfbzac5mhjnxpkxle 113 19 inserted insert VBN work_infoczas7nfbzac5mhjnxpkxle 113 20 into into IN work_infoczas7nfbzac5mhjnxpkxle 113 21 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 113 22 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 113 23 of of IN work_infoczas7nfbzac5mhjnxpkxle 113 24 NPFs npf NNS work_infoczas7nfbzac5mhjnxpkxle 113 25 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 113 26 from from IN work_infoczas7nfbzac5mhjnxpkxle 113 27 a a DT work_infoczas7nfbzac5mhjnxpkxle 113 28 newborn newborn JJ work_infoczas7nfbzac5mhjnxpkxle 113 29 Göttingen Göttingen NNP work_infoczas7nfbzac5mhjnxpkxle 113 30 minipig minipig NNP work_infoczas7nfbzac5mhjnxpkxle 113 31 . . . work_infoczas7nfbzac5mhjnxpkxle 114 1 We -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 114 2 next next RB work_infoczas7nfbzac5mhjnxpkxle 114 3 created create VBD work_infoczas7nfbzac5mhjnxpkxle 114 4 a a DT work_infoczas7nfbzac5mhjnxpkxle 114 5 pool pool NN work_infoczas7nfbzac5mhjnxpkxle 114 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 114 7 clones clone NNS work_infoczas7nfbzac5mhjnxpkxle 114 8 consisting consist VBG work_infoczas7nfbzac5mhjnxpkxle 114 9 of of IN work_infoczas7nfbzac5mhjnxpkxle 114 10 a a DT work_infoczas7nfbzac5mhjnxpkxle 114 11 total total NN work_infoczas7nfbzac5mhjnxpkxle 114 12 of of IN work_infoczas7nfbzac5mhjnxpkxle 114 13 80 80 CD work_infoczas7nfbzac5mhjnxpkxle 114 14 independent independent JJ work_infoczas7nfbzac5mhjnxpkxle 114 15 G418-resistant g418-resistant NN work_infoczas7nfbzac5mhjnxpkxle 114 16 NPF NPF NNP work_infoczas7nfbzac5mhjnxpkxle 114 17 clones clone NNS work_infoczas7nfbzac5mhjnxpkxle 114 18 . . . work_infoczas7nfbzac5mhjnxpkxle 115 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 115 2 pool pool NN work_infoczas7nfbzac5mhjnxpkxle 115 3 was be VBD work_infoczas7nfbzac5mhjnxpkxle 115 4 expanded expand VBN work_infoczas7nfbzac5mhjnxpkxle 115 5 , , , work_infoczas7nfbzac5mhjnxpkxle 115 6 and and CC work_infoczas7nfbzac5mhjnxpkxle 115 7 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 115 8 blastocysts blastocyst NNS work_infoczas7nfbzac5mhjnxpkxle 115 9 were be VBD work_infoczas7nfbzac5mhjnxpkxle 115 10 created create VBN work_infoczas7nfbzac5mhjnxpkxle 115 11 by by IN work_infoczas7nfbzac5mhjnxpkxle 115 12 HMC HMC NNP work_infoczas7nfbzac5mhjnxpkxle 115 13 . . . work_infoczas7nfbzac5mhjnxpkxle 116 1 A a DT work_infoczas7nfbzac5mhjnxpkxle 116 2 total total NN work_infoczas7nfbzac5mhjnxpkxle 116 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 116 4 102 102 CD work_infoczas7nfbzac5mhjnxpkxle 116 5 blastocysts blastocyst NNS work_infoczas7nfbzac5mhjnxpkxle 116 6 were be VBD work_infoczas7nfbzac5mhjnxpkxle 116 7 transferred transfer VBN work_infoczas7nfbzac5mhjnxpkxle 116 8 to to IN work_infoczas7nfbzac5mhjnxpkxle 116 9 the the DT work_infoczas7nfbzac5mhjnxpkxle 116 10 surrogate surrogate JJ work_infoczas7nfbzac5mhjnxpkxle 116 11 sow sow NN work_infoczas7nfbzac5mhjnxpkxle 116 12 . . . work_infoczas7nfbzac5mhjnxpkxle 117 1 Eight eight CD work_infoczas7nfbzac5mhjnxpkxle 117 2 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 117 3 were be VBD work_infoczas7nfbzac5mhjnxpkxle 117 4 born bear VBN work_infoczas7nfbzac5mhjnxpkxle 117 5 alive alive JJ work_infoczas7nfbzac5mhjnxpkxle 117 6 and and CC work_infoczas7nfbzac5mhjnxpkxle 117 7 one one CD work_infoczas7nfbzac5mhjnxpkxle 117 8 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 117 9 was be VBD work_infoczas7nfbzac5mhjnxpkxle 117 10 stillborn stillborn VBN work_infoczas7nfbzac5mhjnxpkxle 117 11 , , , work_infoczas7nfbzac5mhjnxpkxle 117 12 and and CC work_infoczas7nfbzac5mhjnxpkxle 117 13 none none NN work_infoczas7nfbzac5mhjnxpkxle 117 14 of of IN work_infoczas7nfbzac5mhjnxpkxle 117 15 the the DT work_infoczas7nfbzac5mhjnxpkxle 117 16 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 117 17 showed show VBD work_infoczas7nfbzac5mhjnxpkxle 117 18 any any DT work_infoczas7nfbzac5mhjnxpkxle 117 19 sign sign NN work_infoczas7nfbzac5mhjnxpkxle 117 20 of of IN work_infoczas7nfbzac5mhjnxpkxle 117 21 abnormalities abnormality NNS work_infoczas7nfbzac5mhjnxpkxle 117 22 . . . work_infoczas7nfbzac5mhjnxpkxle 118 1 Five five CD work_infoczas7nfbzac5mhjnxpkxle 118 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 118 3 the the DT work_infoczas7nfbzac5mhjnxpkxle 118 4 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 118 5 survived survive VBD work_infoczas7nfbzac5mhjnxpkxle 118 6 past past RB work_infoczas7nfbzac5mhjnxpkxle 118 7 weaning wean VBG work_infoczas7nfbzac5mhjnxpkxle 118 8 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 118 9 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 118 10 . . . work_infoczas7nfbzac5mhjnxpkxle 119 1 1C 1C NNP work_infoczas7nfbzac5mhjnxpkxle 119 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 119 3 . . . work_infoczas7nfbzac5mhjnxpkxle 120 1 7 7 CD work_infoczas7nfbzac5mhjnxpkxle 120 2 Ear Ear NNP work_infoczas7nfbzac5mhjnxpkxle 120 3 cuts cut VBZ work_infoczas7nfbzac5mhjnxpkxle 120 4 from from IN work_infoczas7nfbzac5mhjnxpkxle 120 5 these these DT work_infoczas7nfbzac5mhjnxpkxle 120 6 five five CD work_infoczas7nfbzac5mhjnxpkxle 120 7 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 120 8 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 120 9 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 120 10 # # $ work_infoczas7nfbzac5mhjnxpkxle 120 11 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 120 12 - - SYM work_infoczas7nfbzac5mhjnxpkxle 120 13 9 9 CD work_infoczas7nfbzac5mhjnxpkxle 120 14 , , , work_infoczas7nfbzac5mhjnxpkxle 120 15 and and CC work_infoczas7nfbzac5mhjnxpkxle 120 16 # # $ work_infoczas7nfbzac5mhjnxpkxle 120 17 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 120 18 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 120 19 were be VBD work_infoczas7nfbzac5mhjnxpkxle 120 20 analyzed analyze VBN work_infoczas7nfbzac5mhjnxpkxle 120 21 for for IN work_infoczas7nfbzac5mhjnxpkxle 120 22 fluorescence fluorescence NN work_infoczas7nfbzac5mhjnxpkxle 120 23 under under IN work_infoczas7nfbzac5mhjnxpkxle 120 24 blue blue JJ work_infoczas7nfbzac5mhjnxpkxle 120 25 light light NN work_infoczas7nfbzac5mhjnxpkxle 120 26 , , , work_infoczas7nfbzac5mhjnxpkxle 120 27 and and CC work_infoczas7nfbzac5mhjnxpkxle 120 28 four four CD work_infoczas7nfbzac5mhjnxpkxle 120 29 of of IN work_infoczas7nfbzac5mhjnxpkxle 120 30 the the DT work_infoczas7nfbzac5mhjnxpkxle 120 31 five five CD work_infoczas7nfbzac5mhjnxpkxle 120 32 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 120 33 were be VBD work_infoczas7nfbzac5mhjnxpkxle 120 34 found find VBN work_infoczas7nfbzac5mhjnxpkxle 120 35 to to TO work_infoczas7nfbzac5mhjnxpkxle 120 36 express express VB work_infoczas7nfbzac5mhjnxpkxle 120 37 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 120 38 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 120 39 Fig Fig NNP work_infoczas7nfbzac5mhjnxpkxle 120 40 . . . work_infoczas7nfbzac5mhjnxpkxle 121 1 1D 1D NNP work_infoczas7nfbzac5mhjnxpkxle 121 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 121 3 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 121 4 # # $ work_infoczas7nfbzac5mhjnxpkxle 121 5 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 121 6 - - SYM work_infoczas7nfbzac5mhjnxpkxle 121 7 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 121 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 121 9 # # $ work_infoczas7nfbzac5mhjnxpkxle 121 10 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 121 11 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 121 12 . . . work_infoczas7nfbzac5mhjnxpkxle 122 1 These these DT work_infoczas7nfbzac5mhjnxpkxle 122 2 findings finding NNS work_infoczas7nfbzac5mhjnxpkxle 122 3 were be VBD work_infoczas7nfbzac5mhjnxpkxle 122 4 confirmed confirm VBN work_infoczas7nfbzac5mhjnxpkxle 122 5 by by IN work_infoczas7nfbzac5mhjnxpkxle 122 6 fluorescence fluorescence NN work_infoczas7nfbzac5mhjnxpkxle 122 7 microscopy microscopy JJ work_infoczas7nfbzac5mhjnxpkxle 122 8 of of IN work_infoczas7nfbzac5mhjnxpkxle 122 9 cultured cultured JJ work_infoczas7nfbzac5mhjnxpkxle 122 10 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 122 11 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 122 12 from from IN work_infoczas7nfbzac5mhjnxpkxle 122 13 the the DT work_infoczas7nfbzac5mhjnxpkxle 122 14 ear ear NN work_infoczas7nfbzac5mhjnxpkxle 122 15 biopsies biopsy NNS work_infoczas7nfbzac5mhjnxpkxle 122 16 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 122 17 data datum NNS work_infoczas7nfbzac5mhjnxpkxle 122 18 not not RB work_infoczas7nfbzac5mhjnxpkxle 122 19 shown show VBN work_infoczas7nfbzac5mhjnxpkxle 122 20 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 122 21 . . . work_infoczas7nfbzac5mhjnxpkxle 123 1 Furthermore furthermore RB work_infoczas7nfbzac5mhjnxpkxle 123 2 , , , work_infoczas7nfbzac5mhjnxpkxle 123 3 only only RB work_infoczas7nfbzac5mhjnxpkxle 123 4 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 123 5 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 123 6 from from IN work_infoczas7nfbzac5mhjnxpkxle 123 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 123 8 four four CD work_infoczas7nfbzac5mhjnxpkxle 123 9 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 123 10 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 123 11 positive positive JJ work_infoczas7nfbzac5mhjnxpkxle 123 12 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 123 13 survived survive VBD work_infoczas7nfbzac5mhjnxpkxle 123 14 10 10 CD work_infoczas7nfbzac5mhjnxpkxle 123 15 days day NNS work_infoczas7nfbzac5mhjnxpkxle 123 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 123 17 culturing culturing NN work_infoczas7nfbzac5mhjnxpkxle 123 18 in in IN work_infoczas7nfbzac5mhjnxpkxle 123 19 G418-containing g418-containe VBG work_infoczas7nfbzac5mhjnxpkxle 123 20 medium medium NN work_infoczas7nfbzac5mhjnxpkxle 123 21 , , , work_infoczas7nfbzac5mhjnxpkxle 123 22 whereas whereas IN work_infoczas7nfbzac5mhjnxpkxle 123 23 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 123 24 from from IN work_infoczas7nfbzac5mhjnxpkxle 123 25 the the DT work_infoczas7nfbzac5mhjnxpkxle 123 26 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 123 27 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 123 28 negative negative JJ work_infoczas7nfbzac5mhjnxpkxle 123 29 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 123 30 proved prove VBD work_infoczas7nfbzac5mhjnxpkxle 123 31 not not RB work_infoczas7nfbzac5mhjnxpkxle 123 32 to to TO work_infoczas7nfbzac5mhjnxpkxle 123 33 be be VB work_infoczas7nfbzac5mhjnxpkxle 123 34 G418-resistant g418-resistant JJ work_infoczas7nfbzac5mhjnxpkxle 123 35 . . . work_infoczas7nfbzac5mhjnxpkxle 124 1 These these DT work_infoczas7nfbzac5mhjnxpkxle 124 2 observations observation NNS work_infoczas7nfbzac5mhjnxpkxle 124 3 indicated indicate VBD work_infoczas7nfbzac5mhjnxpkxle 124 4 that that IN work_infoczas7nfbzac5mhjnxpkxle 124 5 at at RB work_infoczas7nfbzac5mhjnxpkxle 124 6 least least RBS work_infoczas7nfbzac5mhjnxpkxle 124 7 one one CD work_infoczas7nfbzac5mhjnxpkxle 124 8 intact intact JJ work_infoczas7nfbzac5mhjnxpkxle 124 9 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 124 10 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 124 11 IRES IRES NNP work_infoczas7nfbzac5mhjnxpkxle 124 12 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 124 13 neo neo NNP work_infoczas7nfbzac5mhjnxpkxle 124 14 r r NN work_infoczas7nfbzac5mhjnxpkxle 124 15 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 124 16 was be VBD work_infoczas7nfbzac5mhjnxpkxle 124 17 present present JJ work_infoczas7nfbzac5mhjnxpkxle 124 18 in in IN work_infoczas7nfbzac5mhjnxpkxle 124 19 four four CD work_infoczas7nfbzac5mhjnxpkxle 124 20 out out IN work_infoczas7nfbzac5mhjnxpkxle 124 21 of of IN work_infoczas7nfbzac5mhjnxpkxle 124 22 five five CD work_infoczas7nfbzac5mhjnxpkxle 124 23 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 124 24 . . . work_infoczas7nfbzac5mhjnxpkxle 125 1 Multiple multiple JJ work_infoczas7nfbzac5mhjnxpkxle 125 2 integrations integration NNS work_infoczas7nfbzac5mhjnxpkxle 125 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 125 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 125 5 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 125 6 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 125 7 in in IN work_infoczas7nfbzac5mhjnxpkxle 125 8 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 125 9 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 125 10 expressing express VBG work_infoczas7nfbzac5mhjnxpkxle 125 11 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 125 12 To to TO work_infoczas7nfbzac5mhjnxpkxle 125 13 characterize characterize VB work_infoczas7nfbzac5mhjnxpkxle 125 14 the the DT work_infoczas7nfbzac5mhjnxpkxle 125 15 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 125 16 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 125 17 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 125 18 in in IN work_infoczas7nfbzac5mhjnxpkxle 125 19 the the DT work_infoczas7nfbzac5mhjnxpkxle 125 20 cloned clone VBN work_infoczas7nfbzac5mhjnxpkxle 125 21 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 125 22 , , , work_infoczas7nfbzac5mhjnxpkxle 125 23 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 125 24 first first RB work_infoczas7nfbzac5mhjnxpkxle 125 25 performed perform VBD work_infoczas7nfbzac5mhjnxpkxle 125 26 Southern southern JJ work_infoczas7nfbzac5mhjnxpkxle 125 27 blot blot NN work_infoczas7nfbzac5mhjnxpkxle 125 28 analysis analysis NN work_infoczas7nfbzac5mhjnxpkxle 125 29 , , , work_infoczas7nfbzac5mhjnxpkxle 125 30 using use VBG work_infoczas7nfbzac5mhjnxpkxle 125 31 a a DT work_infoczas7nfbzac5mhjnxpkxle 125 32 neo neo NN work_infoczas7nfbzac5mhjnxpkxle 125 33 r r NN work_infoczas7nfbzac5mhjnxpkxle 125 34 probe probe NN work_infoczas7nfbzac5mhjnxpkxle 125 35 , , , work_infoczas7nfbzac5mhjnxpkxle 125 36 on on IN work_infoczas7nfbzac5mhjnxpkxle 125 37 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 125 38 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 125 39 isolated isolate VBN work_infoczas7nfbzac5mhjnxpkxle 125 40 from from IN work_infoczas7nfbzac5mhjnxpkxle 125 41 blood blood NN work_infoczas7nfbzac5mhjnxpkxle 125 42 samples sample NNS work_infoczas7nfbzac5mhjnxpkxle 125 43 . . . work_infoczas7nfbzac5mhjnxpkxle 126 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 126 2 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 126 3 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 126 4 was be VBD work_infoczas7nfbzac5mhjnxpkxle 126 5 digested digest VBN work_infoczas7nfbzac5mhjnxpkxle 126 6 with with IN work_infoczas7nfbzac5mhjnxpkxle 126 7 XbaI XbaI NNP work_infoczas7nfbzac5mhjnxpkxle 126 8 , , , work_infoczas7nfbzac5mhjnxpkxle 126 9 which which WDT work_infoczas7nfbzac5mhjnxpkxle 126 10 cuts cut VBZ work_infoczas7nfbzac5mhjnxpkxle 126 11 once once RB work_infoczas7nfbzac5mhjnxpkxle 126 12 in in IN work_infoczas7nfbzac5mhjnxpkxle 126 13 the the DT work_infoczas7nfbzac5mhjnxpkxle 126 14 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 126 15 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 126 16 in in IN work_infoczas7nfbzac5mhjnxpkxle 126 17 the the DT work_infoczas7nfbzac5mhjnxpkxle 126 18 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 126 19 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 126 20 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 126 21 and and CC work_infoczas7nfbzac5mhjnxpkxle 126 22 in in IN work_infoczas7nfbzac5mhjnxpkxle 126 23 the the DT work_infoczas7nfbzac5mhjnxpkxle 126 24 case case NN work_infoczas7nfbzac5mhjnxpkxle 126 25 of of IN work_infoczas7nfbzac5mhjnxpkxle 126 26 a a DT work_infoczas7nfbzac5mhjnxpkxle 126 27 random random JJ work_infoczas7nfbzac5mhjnxpkxle 126 28 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 126 29 in in IN work_infoczas7nfbzac5mhjnxpkxle 126 30 the the DT work_infoczas7nfbzac5mhjnxpkxle 126 31 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 126 32 region region NN work_infoczas7nfbzac5mhjnxpkxle 126 33 flanking flank VBG work_infoczas7nfbzac5mhjnxpkxle 126 34 the the DT work_infoczas7nfbzac5mhjnxpkxle 126 35 right right JJ work_infoczas7nfbzac5mhjnxpkxle 126 36 IR IR NNP work_infoczas7nfbzac5mhjnxpkxle 126 37 of of IN work_infoczas7nfbzac5mhjnxpkxle 126 38 the the DT work_infoczas7nfbzac5mhjnxpkxle 126 39 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 126 40 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 126 41 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 126 42 . . . work_infoczas7nfbzac5mhjnxpkxle 127 1 2A 2a LS work_infoczas7nfbzac5mhjnxpkxle 127 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 127 3 . . . work_infoczas7nfbzac5mhjnxpkxle 128 1 Among among IN work_infoczas7nfbzac5mhjnxpkxle 128 2 the the DT work_infoczas7nfbzac5mhjnxpkxle 128 3 four four CD work_infoczas7nfbzac5mhjnxpkxle 128 4 GFP- GFP- NNP work_infoczas7nfbzac5mhjnxpkxle 128 5 positive positive JJ work_infoczas7nfbzac5mhjnxpkxle 128 6 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 128 7 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 128 8 # # $ work_infoczas7nfbzac5mhjnxpkxle 128 9 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 128 10 - - SYM work_infoczas7nfbzac5mhjnxpkxle 128 11 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 128 12 and and CC work_infoczas7nfbzac5mhjnxpkxle 128 13 # # $ work_infoczas7nfbzac5mhjnxpkxle 128 14 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 128 15 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 128 16 , , , work_infoczas7nfbzac5mhjnxpkxle 128 17 three three CD work_infoczas7nfbzac5mhjnxpkxle 128 18 different different JJ work_infoczas7nfbzac5mhjnxpkxle 128 19 banding banding NN work_infoczas7nfbzac5mhjnxpkxle 128 20 patterns pattern NNS work_infoczas7nfbzac5mhjnxpkxle 128 21 were be VBD work_infoczas7nfbzac5mhjnxpkxle 128 22 observed observe VBN work_infoczas7nfbzac5mhjnxpkxle 128 23 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 128 24 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 128 25 . . . work_infoczas7nfbzac5mhjnxpkxle 129 1 2B 2B NNP work_infoczas7nfbzac5mhjnxpkxle 129 2 , , , work_infoczas7nfbzac5mhjnxpkxle 129 3 lanes lanes NNP work_infoczas7nfbzac5mhjnxpkxle 129 4 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 129 5 - - SYM work_infoczas7nfbzac5mhjnxpkxle 129 6 4 4 CD work_infoczas7nfbzac5mhjnxpkxle 129 7 and and CC work_infoczas7nfbzac5mhjnxpkxle 129 8 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 129 9 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 129 10 . . . work_infoczas7nfbzac5mhjnxpkxle 130 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 130 2 band band NN work_infoczas7nfbzac5mhjnxpkxle 130 3 patterns pattern VBZ work_infoczas7nfbzac5mhjnxpkxle 130 4 in in IN work_infoczas7nfbzac5mhjnxpkxle 130 5 lanes lane NNS work_infoczas7nfbzac5mhjnxpkxle 130 6 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 130 7 and and CC work_infoczas7nfbzac5mhjnxpkxle 130 8 3 3 CD work_infoczas7nfbzac5mhjnxpkxle 130 9 were be VBD work_infoczas7nfbzac5mhjnxpkxle 130 10 identical identical JJ work_infoczas7nfbzac5mhjnxpkxle 130 11 and and CC work_infoczas7nfbzac5mhjnxpkxle 130 12 suggested suggest VBD work_infoczas7nfbzac5mhjnxpkxle 130 13 a a DT work_infoczas7nfbzac5mhjnxpkxle 130 14 total total NN work_infoczas7nfbzac5mhjnxpkxle 130 15 of of IN work_infoczas7nfbzac5mhjnxpkxle 130 16 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 130 17 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 130 18 . . . work_infoczas7nfbzac5mhjnxpkxle 131 1 Hence hence RB work_infoczas7nfbzac5mhjnxpkxle 131 2 , , , work_infoczas7nfbzac5mhjnxpkxle 131 3 the the DT work_infoczas7nfbzac5mhjnxpkxle 131 4 two two CD work_infoczas7nfbzac5mhjnxpkxle 131 5 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 131 6 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 131 7 # # $ work_infoczas7nfbzac5mhjnxpkxle 131 8 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 131 9 and and CC work_infoczas7nfbzac5mhjnxpkxle 131 10 # # $ work_infoczas7nfbzac5mhjnxpkxle 131 11 7 7 CD work_infoczas7nfbzac5mhjnxpkxle 131 12 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 131 13 originated originate VBD work_infoczas7nfbzac5mhjnxpkxle 131 14 from from IN work_infoczas7nfbzac5mhjnxpkxle 131 15 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 131 16 from from IN work_infoczas7nfbzac5mhjnxpkxle 131 17 the the DT work_infoczas7nfbzac5mhjnxpkxle 131 18 same same JJ work_infoczas7nfbzac5mhjnxpkxle 131 19 NPF NPF NNP work_infoczas7nfbzac5mhjnxpkxle 131 20 clone clone NN work_infoczas7nfbzac5mhjnxpkxle 131 21 . . . work_infoczas7nfbzac5mhjnxpkxle 132 1 Two two CD work_infoczas7nfbzac5mhjnxpkxle 132 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 132 3 the the DT work_infoczas7nfbzac5mhjnxpkxle 132 4 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 132 5 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 132 6 # # $ work_infoczas7nfbzac5mhjnxpkxle 132 7 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 132 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 132 9 # # $ work_infoczas7nfbzac5mhjnxpkxle 132 10 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 132 11 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 132 12 , , , work_infoczas7nfbzac5mhjnxpkxle 132 13 each each DT work_infoczas7nfbzac5mhjnxpkxle 132 14 containing contain VBG work_infoczas7nfbzac5mhjnxpkxle 132 15 eight eight CD work_infoczas7nfbzac5mhjnxpkxle 132 16 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 132 17 , , , work_infoczas7nfbzac5mhjnxpkxle 132 18 had have VBD work_infoczas7nfbzac5mhjnxpkxle 132 19 almost almost RB work_infoczas7nfbzac5mhjnxpkxle 132 20 identical identical JJ work_infoczas7nfbzac5mhjnxpkxle 132 21 banding banding NN work_infoczas7nfbzac5mhjnxpkxle 132 22 patterns pattern NNS work_infoczas7nfbzac5mhjnxpkxle 132 23 except except IN work_infoczas7nfbzac5mhjnxpkxle 132 24 for for IN work_infoczas7nfbzac5mhjnxpkxle 132 25 two two CD work_infoczas7nfbzac5mhjnxpkxle 132 26 single single JJ work_infoczas7nfbzac5mhjnxpkxle 132 27 bands band NNS work_infoczas7nfbzac5mhjnxpkxle 132 28 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 132 29 position position NN work_infoczas7nfbzac5mhjnxpkxle 132 30 marked mark VBN work_infoczas7nfbzac5mhjnxpkxle 132 31 with with IN work_infoczas7nfbzac5mhjnxpkxle 132 32 arrows arrow NNS work_infoczas7nfbzac5mhjnxpkxle 132 33 in in IN work_infoczas7nfbzac5mhjnxpkxle 132 34 Fig Fig NNP work_infoczas7nfbzac5mhjnxpkxle 132 35 . . . work_infoczas7nfbzac5mhjnxpkxle 133 1 2B 2b LS work_infoczas7nfbzac5mhjnxpkxle 133 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 133 3 that that WDT work_infoczas7nfbzac5mhjnxpkxle 133 4 varied vary VBD work_infoczas7nfbzac5mhjnxpkxle 133 5 between between IN work_infoczas7nfbzac5mhjnxpkxle 133 6 the the DT work_infoczas7nfbzac5mhjnxpkxle 133 7 two two CD work_infoczas7nfbzac5mhjnxpkxle 133 8 animals animal NNS work_infoczas7nfbzac5mhjnxpkxle 133 9 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 133 10 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 133 11 . . . work_infoczas7nfbzac5mhjnxpkxle 134 1 2B 2B NNP work_infoczas7nfbzac5mhjnxpkxle 134 2 , , , work_infoczas7nfbzac5mhjnxpkxle 134 3 lanes lanes NNP work_infoczas7nfbzac5mhjnxpkxle 134 4 4 4 CD work_infoczas7nfbzac5mhjnxpkxle 134 5 and and CC work_infoczas7nfbzac5mhjnxpkxle 134 6 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 134 7 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 134 8 . . . work_infoczas7nfbzac5mhjnxpkxle 135 1 These these DT work_infoczas7nfbzac5mhjnxpkxle 135 2 two two CD work_infoczas7nfbzac5mhjnxpkxle 135 3 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 135 4 were be VBD work_infoczas7nfbzac5mhjnxpkxle 135 5 most most RBS work_infoczas7nfbzac5mhjnxpkxle 135 6 likely likely RB work_infoczas7nfbzac5mhjnxpkxle 135 7 generated generate VBN work_infoczas7nfbzac5mhjnxpkxle 135 8 from from IN work_infoczas7nfbzac5mhjnxpkxle 135 9 the the DT work_infoczas7nfbzac5mhjnxpkxle 135 10 same same JJ work_infoczas7nfbzac5mhjnxpkxle 135 11 NPF NPF NNP work_infoczas7nfbzac5mhjnxpkxle 135 12 clone clone NN work_infoczas7nfbzac5mhjnxpkxle 135 13 which which WDT work_infoczas7nfbzac5mhjnxpkxle 135 14 happened happen VBD work_infoczas7nfbzac5mhjnxpkxle 135 15 to to TO work_infoczas7nfbzac5mhjnxpkxle 135 16 be be VB work_infoczas7nfbzac5mhjnxpkxle 135 17 polyclonal polyclonal JJ work_infoczas7nfbzac5mhjnxpkxle 135 18 due due IN work_infoczas7nfbzac5mhjnxpkxle 135 19 to to IN work_infoczas7nfbzac5mhjnxpkxle 135 20 late late JJ work_infoczas7nfbzac5mhjnxpkxle 135 21 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 135 22 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 135 23 or or CC work_infoczas7nfbzac5mhjnxpkxle 135 24 , , , work_infoczas7nfbzac5mhjnxpkxle 135 25 alternatively alternatively RB work_infoczas7nfbzac5mhjnxpkxle 135 26 , , , work_infoczas7nfbzac5mhjnxpkxle 135 27 due due JJ work_infoczas7nfbzac5mhjnxpkxle 135 28 to to IN work_infoczas7nfbzac5mhjnxpkxle 135 29 re re NN work_infoczas7nfbzac5mhjnxpkxle 135 30 - - NN work_infoczas7nfbzac5mhjnxpkxle 135 31 mobilization mobilization NN work_infoczas7nfbzac5mhjnxpkxle 135 32 of of IN work_infoczas7nfbzac5mhjnxpkxle 135 33 an an DT work_infoczas7nfbzac5mhjnxpkxle 135 34 inserted insert VBN work_infoczas7nfbzac5mhjnxpkxle 135 35 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 135 36 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 135 37 during during IN work_infoczas7nfbzac5mhjnxpkxle 135 38 clone clone NN work_infoczas7nfbzac5mhjnxpkxle 135 39 development development NN work_infoczas7nfbzac5mhjnxpkxle 135 40 and and CC work_infoczas7nfbzac5mhjnxpkxle 135 41 selection selection NN work_infoczas7nfbzac5mhjnxpkxle 135 42 . . . work_infoczas7nfbzac5mhjnxpkxle 136 1 Interestingly interestingly RB work_infoczas7nfbzac5mhjnxpkxle 136 2 , , , work_infoczas7nfbzac5mhjnxpkxle 136 3 the the DT work_infoczas7nfbzac5mhjnxpkxle 136 4 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 136 5 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 136 6 negative negative JJ work_infoczas7nfbzac5mhjnxpkxle 136 7 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 136 8 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 136 9 # # $ work_infoczas7nfbzac5mhjnxpkxle 136 10 9 9 CD work_infoczas7nfbzac5mhjnxpkxle 136 11 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 136 12 was be VBD work_infoczas7nfbzac5mhjnxpkxle 136 13 indeed indeed RB work_infoczas7nfbzac5mhjnxpkxle 136 14 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 136 15 but but CC work_infoczas7nfbzac5mhjnxpkxle 136 16 contained contain VBN work_infoczas7nfbzac5mhjnxpkxle 136 17 only only RB work_infoczas7nfbzac5mhjnxpkxle 136 18 randomly randomly RB work_infoczas7nfbzac5mhjnxpkxle 136 19 inserted insert VBN work_infoczas7nfbzac5mhjnxpkxle 136 20 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 136 21 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 136 22 , , , work_infoczas7nfbzac5mhjnxpkxle 136 23 as as IN work_infoczas7nfbzac5mhjnxpkxle 136 24 indicated indicate VBN work_infoczas7nfbzac5mhjnxpkxle 136 25 by by IN work_infoczas7nfbzac5mhjnxpkxle 136 26 the the DT work_infoczas7nfbzac5mhjnxpkxle 136 27 detection detection NN work_infoczas7nfbzac5mhjnxpkxle 136 28 of of IN work_infoczas7nfbzac5mhjnxpkxle 136 29 a a DT work_infoczas7nfbzac5mhjnxpkxle 136 30 4.5-kb 4.5-kb CD work_infoczas7nfbzac5mhjnxpkxle 136 31 fragment fragment NN work_infoczas7nfbzac5mhjnxpkxle 136 32 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 136 33 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 136 34 . . . work_infoczas7nfbzac5mhjnxpkxle 137 1 2B 2B NNP work_infoczas7nfbzac5mhjnxpkxle 137 2 , , , work_infoczas7nfbzac5mhjnxpkxle 137 3 lane lane NNP work_infoczas7nfbzac5mhjnxpkxle 137 4 5 5 CD work_infoczas7nfbzac5mhjnxpkxle 137 5 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 137 6 . . . work_infoczas7nfbzac5mhjnxpkxle 138 1 Our -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 138 2 data datum NNS work_infoczas7nfbzac5mhjnxpkxle 138 3 suggested suggest VBD work_infoczas7nfbzac5mhjnxpkxle 138 4 that that IN work_infoczas7nfbzac5mhjnxpkxle 138 5 the the DT work_infoczas7nfbzac5mhjnxpkxle 138 6 five five CD work_infoczas7nfbzac5mhjnxpkxle 138 7 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 138 8 were be VBD work_infoczas7nfbzac5mhjnxpkxle 138 9 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 138 10 from from IN work_infoczas7nfbzac5mhjnxpkxle 138 11 a a DT work_infoczas7nfbzac5mhjnxpkxle 138 12 total total NN work_infoczas7nfbzac5mhjnxpkxle 138 13 of of IN work_infoczas7nfbzac5mhjnxpkxle 138 14 three three CD work_infoczas7nfbzac5mhjnxpkxle 138 15 NPF NPF NNP work_infoczas7nfbzac5mhjnxpkxle 138 16 clones clone NNS work_infoczas7nfbzac5mhjnxpkxle 138 17 selected select VBN work_infoczas7nfbzac5mhjnxpkxle 138 18 among among IN work_infoczas7nfbzac5mhjnxpkxle 138 19 the the DT work_infoczas7nfbzac5mhjnxpkxle 138 20 80 80 CD work_infoczas7nfbzac5mhjnxpkxle 138 21 clones clone NNS work_infoczas7nfbzac5mhjnxpkxle 138 22 of of IN work_infoczas7nfbzac5mhjnxpkxle 138 23 the the DT work_infoczas7nfbzac5mhjnxpkxle 138 24 original original JJ work_infoczas7nfbzac5mhjnxpkxle 138 25 pool pool NN work_infoczas7nfbzac5mhjnxpkxle 138 26 . . . work_infoczas7nfbzac5mhjnxpkxle 139 1 This this DT work_infoczas7nfbzac5mhjnxpkxle 139 2 lends lend VBZ work_infoczas7nfbzac5mhjnxpkxle 139 3 support support NN work_infoczas7nfbzac5mhjnxpkxle 139 4 to to IN work_infoczas7nfbzac5mhjnxpkxle 139 5 the the DT work_infoczas7nfbzac5mhjnxpkxle 139 6 notion notion NN work_infoczas7nfbzac5mhjnxpkxle 139 7 that that IN work_infoczas7nfbzac5mhjnxpkxle 139 8 a a DT work_infoczas7nfbzac5mhjnxpkxle 139 9 strong strong JJ work_infoczas7nfbzac5mhjnxpkxle 139 10 selection selection NN work_infoczas7nfbzac5mhjnxpkxle 139 11 pressure pressure NN work_infoczas7nfbzac5mhjnxpkxle 139 12 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 139 13 evident evident JJ work_infoczas7nfbzac5mhjnxpkxle 139 14 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 139 15 at at IN work_infoczas7nfbzac5mhjnxpkxle 139 16 one one CD work_infoczas7nfbzac5mhjnxpkxle 139 17 or or CC work_infoczas7nfbzac5mhjnxpkxle 139 18 more more JJR work_infoczas7nfbzac5mhjnxpkxle 139 19 steps step NNS work_infoczas7nfbzac5mhjnxpkxle 139 20 of of IN work_infoczas7nfbzac5mhjnxpkxle 139 21 the the DT work_infoczas7nfbzac5mhjnxpkxle 139 22 cloning clone VBG work_infoczas7nfbzac5mhjnxpkxle 139 23 procedure procedure NN work_infoczas7nfbzac5mhjnxpkxle 139 24 including include VBG work_infoczas7nfbzac5mhjnxpkxle 139 25 expansion expansion NN work_infoczas7nfbzac5mhjnxpkxle 139 26 of of IN work_infoczas7nfbzac5mhjnxpkxle 139 27 pooled pool VBN work_infoczas7nfbzac5mhjnxpkxle 139 28 clones clone NNS work_infoczas7nfbzac5mhjnxpkxle 139 29 , , , work_infoczas7nfbzac5mhjnxpkxle 139 30 generation generation NN work_infoczas7nfbzac5mhjnxpkxle 139 31 of of IN work_infoczas7nfbzac5mhjnxpkxle 139 32 blastocysts blastocyst NNS work_infoczas7nfbzac5mhjnxpkxle 139 33 , , , work_infoczas7nfbzac5mhjnxpkxle 139 34 and/or and/or CC work_infoczas7nfbzac5mhjnxpkxle 139 35 embryo embryo NN work_infoczas7nfbzac5mhjnxpkxle 139 36 and and CC work_infoczas7nfbzac5mhjnxpkxle 139 37 fetal fetal JJ work_infoczas7nfbzac5mhjnxpkxle 139 38 development development NN work_infoczas7nfbzac5mhjnxpkxle 139 39 . . . work_infoczas7nfbzac5mhjnxpkxle 140 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 140 2 Southern southern JJ work_infoczas7nfbzac5mhjnxpkxle 140 3 blot blot NN work_infoczas7nfbzac5mhjnxpkxle 140 4 analysis analysis NN work_infoczas7nfbzac5mhjnxpkxle 140 5 suggested suggest VBD work_infoczas7nfbzac5mhjnxpkxle 140 6 that that IN work_infoczas7nfbzac5mhjnxpkxle 140 7 random random JJ work_infoczas7nfbzac5mhjnxpkxle 140 8 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 140 9 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 140 10 caused cause VBN work_infoczas7nfbzac5mhjnxpkxle 140 11 by by IN work_infoczas7nfbzac5mhjnxpkxle 140 12 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 140 13 breakage breakage NN work_infoczas7nfbzac5mhjnxpkxle 140 14 in in IN work_infoczas7nfbzac5mhjnxpkxle 140 15 the the DT work_infoczas7nfbzac5mhjnxpkxle 140 16 region region NN work_infoczas7nfbzac5mhjnxpkxle 140 17 outside outside IN work_infoczas7nfbzac5mhjnxpkxle 140 18 the the DT work_infoczas7nfbzac5mhjnxpkxle 140 19 XbaI XbaI NNP work_infoczas7nfbzac5mhjnxpkxle 140 20 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 140 21 flanked flank VBN work_infoczas7nfbzac5mhjnxpkxle 140 22 GIN GIN NNP work_infoczas7nfbzac5mhjnxpkxle 140 23 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 140 24 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 140 25 resulting result VBG work_infoczas7nfbzac5mhjnxpkxle 140 26 in in IN work_infoczas7nfbzac5mhjnxpkxle 140 27 a a DT work_infoczas7nfbzac5mhjnxpkxle 140 28 4.5-kb 4.5-kb CD work_infoczas7nfbzac5mhjnxpkxle 140 29 fragment fragment NN work_infoczas7nfbzac5mhjnxpkxle 140 30 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 140 31 occurred occur VBD work_infoczas7nfbzac5mhjnxpkxle 140 32 in in IN work_infoczas7nfbzac5mhjnxpkxle 140 33 all all DT work_infoczas7nfbzac5mhjnxpkxle 140 34 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 140 35 but but CC work_infoczas7nfbzac5mhjnxpkxle 140 36 was be VBD work_infoczas7nfbzac5mhjnxpkxle 140 37 prominent prominent JJ work_infoczas7nfbzac5mhjnxpkxle 140 38 only only RB work_infoczas7nfbzac5mhjnxpkxle 140 39 in in IN work_infoczas7nfbzac5mhjnxpkxle 140 40 the the DT work_infoczas7nfbzac5mhjnxpkxle 140 41 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 140 42 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 140 43 negative negative JJ work_infoczas7nfbzac5mhjnxpkxle 140 44 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 140 45 . . . work_infoczas7nfbzac5mhjnxpkxle 141 1 In in IN work_infoczas7nfbzac5mhjnxpkxle 141 2 this this DT work_infoczas7nfbzac5mhjnxpkxle 141 3 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 141 4 , , , work_infoczas7nfbzac5mhjnxpkxle 141 5 the the DT work_infoczas7nfbzac5mhjnxpkxle 141 6 4.5-kb 4.5-kb CD work_infoczas7nfbzac5mhjnxpkxle 141 7 band band NN work_infoczas7nfbzac5mhjnxpkxle 141 8 was be VBD work_infoczas7nfbzac5mhjnxpkxle 141 9 representative representative JJ work_infoczas7nfbzac5mhjnxpkxle 141 10 of of IN work_infoczas7nfbzac5mhjnxpkxle 141 11 two two CD work_infoczas7nfbzac5mhjnxpkxle 141 12 independent independent JJ work_infoczas7nfbzac5mhjnxpkxle 141 13 random random JJ work_infoczas7nfbzac5mhjnxpkxle 141 14 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 141 15 , , , work_infoczas7nfbzac5mhjnxpkxle 141 16 which which WDT work_infoczas7nfbzac5mhjnxpkxle 141 17 was be VBD work_infoczas7nfbzac5mhjnxpkxle 141 18 confirmed confirm VBN work_infoczas7nfbzac5mhjnxpkxle 141 19 by by IN work_infoczas7nfbzac5mhjnxpkxle 141 20 the the DT work_infoczas7nfbzac5mhjnxpkxle 141 21 presence presence NN work_infoczas7nfbzac5mhjnxpkxle 141 22 of of IN work_infoczas7nfbzac5mhjnxpkxle 141 23 two two CD work_infoczas7nfbzac5mhjnxpkxle 141 24 separate separate JJ work_infoczas7nfbzac5mhjnxpkxle 141 25 bands band NNS work_infoczas7nfbzac5mhjnxpkxle 141 26 on on IN work_infoczas7nfbzac5mhjnxpkxle 141 27 a a DT work_infoczas7nfbzac5mhjnxpkxle 141 28 Southern southern JJ work_infoczas7nfbzac5mhjnxpkxle 141 29 blot blot NN work_infoczas7nfbzac5mhjnxpkxle 141 30 , , , work_infoczas7nfbzac5mhjnxpkxle 141 31 where where WRB work_infoczas7nfbzac5mhjnxpkxle 141 32 enzymes enzyme NNS work_infoczas7nfbzac5mhjnxpkxle 141 33 cutting cut VBG work_infoczas7nfbzac5mhjnxpkxle 141 34 within within IN work_infoczas7nfbzac5mhjnxpkxle 141 35 the the DT work_infoczas7nfbzac5mhjnxpkxle 141 36 Ubi Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 141 37 promoter promoter NN work_infoczas7nfbzac5mhjnxpkxle 141 38 and and CC work_infoczas7nfbzac5mhjnxpkxle 141 39 within within IN work_infoczas7nfbzac5mhjnxpkxle 141 40 the the DT work_infoczas7nfbzac5mhjnxpkxle 141 41 IRES IRES NNP work_infoczas7nfbzac5mhjnxpkxle 141 42 sequence sequence NN work_infoczas7nfbzac5mhjnxpkxle 141 43 were be VBD work_infoczas7nfbzac5mhjnxpkxle 141 44 used use VBN work_infoczas7nfbzac5mhjnxpkxle 141 45 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 141 46 data datum NNS work_infoczas7nfbzac5mhjnxpkxle 141 47 not not RB work_infoczas7nfbzac5mhjnxpkxle 141 48 shown show VBN work_infoczas7nfbzac5mhjnxpkxle 141 49 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 141 50 . . . work_infoczas7nfbzac5mhjnxpkxle 142 1 We -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 142 2 have have VBP work_infoczas7nfbzac5mhjnxpkxle 142 3 previously previously RB work_infoczas7nfbzac5mhjnxpkxle 142 4 found find VBN work_infoczas7nfbzac5mhjnxpkxle 142 5 evidence evidence NN work_infoczas7nfbzac5mhjnxpkxle 142 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 142 7 random random JJ work_infoczas7nfbzac5mhjnxpkxle 142 8 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 142 9 of of IN work_infoczas7nfbzac5mhjnxpkxle 142 10 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 142 11 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 142 12 encoding encode VBG work_infoczas7nfbzac5mhjnxpkxle 142 13 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 142 14 sequences sequence NNS work_infoczas7nfbzac5mhjnxpkxle 142 15 in in IN work_infoczas7nfbzac5mhjnxpkxle 142 16 a a DT work_infoczas7nfbzac5mhjnxpkxle 142 17 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 142 18 cloned clone VBN work_infoczas7nfbzac5mhjnxpkxle 142 19 Göttingen Göttingen NNP work_infoczas7nfbzac5mhjnxpkxle 142 20 minipig minipig NNP work_infoczas7nfbzac5mhjnxpkxle 142 21 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 142 22 J. J. NNP work_infoczas7nfbzac5mhjnxpkxle 142 23 E. E. NNP work_infoczas7nfbzac5mhjnxpkxle 142 24 Jakobsen Jakobsen NNP work_infoczas7nfbzac5mhjnxpkxle 142 25 , , , work_infoczas7nfbzac5mhjnxpkxle 142 26 A. A. NNP work_infoczas7nfbzac5mhjnxpkxle 142 27 L. L. NNP work_infoczas7nfbzac5mhjnxpkxle 142 28 Jørgensen Jørgensen NNP work_infoczas7nfbzac5mhjnxpkxle 142 29 , , , work_infoczas7nfbzac5mhjnxpkxle 142 30 A. A. NNP work_infoczas7nfbzac5mhjnxpkxle 142 31 L. L. NNP work_infoczas7nfbzac5mhjnxpkxle 142 32 Nielsen Nielsen NNP work_infoczas7nfbzac5mhjnxpkxle 142 33 , , , work_infoczas7nfbzac5mhjnxpkxle 142 34 J. J. NNP work_infoczas7nfbzac5mhjnxpkxle 142 35 G. G. NNP work_infoczas7nfbzac5mhjnxpkxle 142 36 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 142 37 ; ; , work_infoczas7nfbzac5mhjnxpkxle 142 38 unpublished unpublished JJ work_infoczas7nfbzac5mhjnxpkxle 142 39 observations observation NNS work_infoczas7nfbzac5mhjnxpkxle 142 40 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 142 41 . . . work_infoczas7nfbzac5mhjnxpkxle 143 1 A a DT work_infoczas7nfbzac5mhjnxpkxle 143 2 sample sample NN work_infoczas7nfbzac5mhjnxpkxle 143 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 143 4 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 143 5 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 143 6 from from IN work_infoczas7nfbzac5mhjnxpkxle 143 7 this this DT work_infoczas7nfbzac5mhjnxpkxle 143 8 animal animal NN work_infoczas7nfbzac5mhjnxpkxle 143 9 was be VBD work_infoczas7nfbzac5mhjnxpkxle 143 10 utilized utilize VBN work_infoczas7nfbzac5mhjnxpkxle 143 11 as as IN work_infoczas7nfbzac5mhjnxpkxle 143 12 a a DT work_infoczas7nfbzac5mhjnxpkxle 143 13 positive positive JJ work_infoczas7nfbzac5mhjnxpkxle 143 14 control control NN work_infoczas7nfbzac5mhjnxpkxle 143 15 in in IN work_infoczas7nfbzac5mhjnxpkxle 143 16 a a DT work_infoczas7nfbzac5mhjnxpkxle 143 17 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 143 18 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 143 19 based base VBN work_infoczas7nfbzac5mhjnxpkxle 143 20 analysis analysis NN work_infoczas7nfbzac5mhjnxpkxle 143 21 of of IN work_infoczas7nfbzac5mhjnxpkxle 143 22 the the DT work_infoczas7nfbzac5mhjnxpkxle 143 23 presence presence NN work_infoczas7nfbzac5mhjnxpkxle 143 24 of of IN work_infoczas7nfbzac5mhjnxpkxle 143 25 such such JJ work_infoczas7nfbzac5mhjnxpkxle 143 26 sequences sequence NNS work_infoczas7nfbzac5mhjnxpkxle 143 27 in in IN work_infoczas7nfbzac5mhjnxpkxle 143 28 the the DT work_infoczas7nfbzac5mhjnxpkxle 143 29 five five CD work_infoczas7nfbzac5mhjnxpkxle 143 30 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 143 31 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 143 32 studied study VBN work_infoczas7nfbzac5mhjnxpkxle 143 33 here here RB work_infoczas7nfbzac5mhjnxpkxle 143 34 . . . work_infoczas7nfbzac5mhjnxpkxle 144 1 We -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 144 2 did do VBD work_infoczas7nfbzac5mhjnxpkxle 144 3 not not RB work_infoczas7nfbzac5mhjnxpkxle 144 4 find find VB work_infoczas7nfbzac5mhjnxpkxle 144 5 any any DT work_infoczas7nfbzac5mhjnxpkxle 144 6 evidence evidence NN work_infoczas7nfbzac5mhjnxpkxle 144 7 of of IN work_infoczas7nfbzac5mhjnxpkxle 144 8 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 144 9 of of IN work_infoczas7nfbzac5mhjnxpkxle 144 10 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 144 11 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 144 12 encoding encode VBG work_infoczas7nfbzac5mhjnxpkxle 144 13 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 144 14 in in IN work_infoczas7nfbzac5mhjnxpkxle 144 15 any any DT work_infoczas7nfbzac5mhjnxpkxle 144 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 144 17 the the DT work_infoczas7nfbzac5mhjnxpkxle 144 18 five five CD work_infoczas7nfbzac5mhjnxpkxle 144 19 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 144 20 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 144 21 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 144 22 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 144 23 . . . work_infoczas7nfbzac5mhjnxpkxle 145 1 2C 2C NNP work_infoczas7nfbzac5mhjnxpkxle 145 2 , , , work_infoczas7nfbzac5mhjnxpkxle 145 3 lanes lane VBZ work_infoczas7nfbzac5mhjnxpkxle 145 4 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 145 5 - - SYM work_infoczas7nfbzac5mhjnxpkxle 145 6 5 5 CD work_infoczas7nfbzac5mhjnxpkxle 145 7 and and CC work_infoczas7nfbzac5mhjnxpkxle 145 8 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 145 9 - - SYM work_infoczas7nfbzac5mhjnxpkxle 145 10 12 12 CD work_infoczas7nfbzac5mhjnxpkxle 145 11 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 145 12 or or CC work_infoczas7nfbzac5mhjnxpkxle 145 13 a a DT work_infoczas7nfbzac5mhjnxpkxle 145 14 negative negative JJ work_infoczas7nfbzac5mhjnxpkxle 145 15 control control NN work_infoczas7nfbzac5mhjnxpkxle 145 16 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 145 17 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 145 18 . . . work_infoczas7nfbzac5mhjnxpkxle 146 1 2C 2C NNP work_infoczas7nfbzac5mhjnxpkxle 146 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 146 3 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 146 4 # # $ work_infoczas7nfbzac5mhjnxpkxle 146 5 78 78 CD work_infoczas7nfbzac5mhjnxpkxle 146 6 , , , work_infoczas7nfbzac5mhjnxpkxle 146 7 lanes lane VBZ work_infoczas7nfbzac5mhjnxpkxle 146 8 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 146 9 and and CC work_infoczas7nfbzac5mhjnxpkxle 146 10 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 146 11 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 146 12 , , , work_infoczas7nfbzac5mhjnxpkxle 146 13 whereas whereas IN work_infoczas7nfbzac5mhjnxpkxle 146 14 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 146 15 analysis analysis NN work_infoczas7nfbzac5mhjnxpkxle 146 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 146 17 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 146 18 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 146 19 from from IN work_infoczas7nfbzac5mhjnxpkxle 146 20 the the DT work_infoczas7nfbzac5mhjnxpkxle 146 21 positive positive JJ work_infoczas7nfbzac5mhjnxpkxle 146 22 control control NN work_infoczas7nfbzac5mhjnxpkxle 146 23 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 146 24 showed show VBD work_infoczas7nfbzac5mhjnxpkxle 146 25 evidence evidence NN work_infoczas7nfbzac5mhjnxpkxle 146 26 of of IN work_infoczas7nfbzac5mhjnxpkxle 146 27 the the DT work_infoczas7nfbzac5mhjnxpkxle 146 28 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 146 29 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 146 30 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 146 31 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 146 32 . . . work_infoczas7nfbzac5mhjnxpkxle 147 1 2C 2C NNP work_infoczas7nfbzac5mhjnxpkxle 147 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 147 3 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 147 4 # # $ work_infoczas7nfbzac5mhjnxpkxle 147 5 58 58 CD work_infoczas7nfbzac5mhjnxpkxle 147 6 , , , work_infoczas7nfbzac5mhjnxpkxle 147 7 lanes lane VBZ work_infoczas7nfbzac5mhjnxpkxle 147 8 7 7 CD work_infoczas7nfbzac5mhjnxpkxle 147 9 and and CC work_infoczas7nfbzac5mhjnxpkxle 147 10 14 14 CD work_infoczas7nfbzac5mhjnxpkxle 147 11 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 147 12 . . . work_infoczas7nfbzac5mhjnxpkxle 148 1 Evidence evidence NN work_infoczas7nfbzac5mhjnxpkxle 148 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 148 3 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 148 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 148 5 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 148 6 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 148 7 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 148 8 by by IN work_infoczas7nfbzac5mhjnxpkxle 148 9 analysis analysis NN work_infoczas7nfbzac5mhjnxpkxle 148 10 of of IN work_infoczas7nfbzac5mhjnxpkxle 148 11 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 148 12 sites site NNS work_infoczas7nfbzac5mhjnxpkxle 148 13 in in IN work_infoczas7nfbzac5mhjnxpkxle 148 14 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 148 15 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 148 16 To to TO work_infoczas7nfbzac5mhjnxpkxle 148 17 confirm confirm VB work_infoczas7nfbzac5mhjnxpkxle 148 18 that that IN work_infoczas7nfbzac5mhjnxpkxle 148 19 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 148 20 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 148 21 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 148 22 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 148 23 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 148 24 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 148 25 was be VBD work_infoczas7nfbzac5mhjnxpkxle 148 26 a a DT work_infoczas7nfbzac5mhjnxpkxle 148 27 prevalent prevalent JJ work_infoczas7nfbzac5mhjnxpkxle 148 28 mechanism mechanism NN work_infoczas7nfbzac5mhjnxpkxle 148 29 of of IN work_infoczas7nfbzac5mhjnxpkxle 148 30 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 148 31 during during IN work_infoczas7nfbzac5mhjnxpkxle 148 32 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 148 33 , , , work_infoczas7nfbzac5mhjnxpkxle 148 34 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 148 35 performed perform VBD work_infoczas7nfbzac5mhjnxpkxle 148 36 long long JJ work_infoczas7nfbzac5mhjnxpkxle 148 37 distance distance NN work_infoczas7nfbzac5mhjnxpkxle 148 38 inverse inverse NN work_infoczas7nfbzac5mhjnxpkxle 148 39 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 148 40 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 148 41 LDI LDI NNP work_infoczas7nfbzac5mhjnxpkxle 148 42 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 148 43 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 148 44 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 148 45 on on IN work_infoczas7nfbzac5mhjnxpkxle 148 46 one one CD work_infoczas7nfbzac5mhjnxpkxle 148 47 of of IN work_infoczas7nfbzac5mhjnxpkxle 148 48 the the DT work_infoczas7nfbzac5mhjnxpkxle 148 49 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 148 50 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 148 51 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 148 52 # # NNP work_infoczas7nfbzac5mhjnxpkxle 148 53 8) 8) CD work_infoczas7nfbzac5mhjnxpkxle 148 54 containing contain VBG work_infoczas7nfbzac5mhjnxpkxle 148 55 eight eight CD work_infoczas7nfbzac5mhjnxpkxle 148 56 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 148 57 to to TO work_infoczas7nfbzac5mhjnxpkxle 148 58 identify identify VB work_infoczas7nfbzac5mhjnxpkxle 148 59 junction junction NN work_infoczas7nfbzac5mhjnxpkxle 148 60 sites site NNS work_infoczas7nfbzac5mhjnxpkxle 148 61 between between IN work_infoczas7nfbzac5mhjnxpkxle 148 62 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 148 63 and and CC work_infoczas7nfbzac5mhjnxpkxle 148 64 genomic genomic NNP work_infoczas7nfbzac5mhjnxpkxle 148 65 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 148 66 . . . work_infoczas7nfbzac5mhjnxpkxle 149 1 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 149 2 amplification amplification NN work_infoczas7nfbzac5mhjnxpkxle 149 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 149 4 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 149 5 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 149 6 digested digest VBN work_infoczas7nfbzac5mhjnxpkxle 149 7 with with IN work_infoczas7nfbzac5mhjnxpkxle 149 8 either either CC work_infoczas7nfbzac5mhjnxpkxle 149 9 BsrGI BsrGI NNP work_infoczas7nfbzac5mhjnxpkxle 149 10 or or CC work_infoczas7nfbzac5mhjnxpkxle 149 11 BsrGI BsrGI NNP work_infoczas7nfbzac5mhjnxpkxle 149 12 / / SYM work_infoczas7nfbzac5mhjnxpkxle 149 13 NdeI NdeI NNP work_infoczas7nfbzac5mhjnxpkxle 149 14 resulted result VBD work_infoczas7nfbzac5mhjnxpkxle 149 15 in in IN work_infoczas7nfbzac5mhjnxpkxle 149 16 a a DT work_infoczas7nfbzac5mhjnxpkxle 149 17 total total NN work_infoczas7nfbzac5mhjnxpkxle 149 18 of of IN work_infoczas7nfbzac5mhjnxpkxle 149 19 five five CD work_infoczas7nfbzac5mhjnxpkxle 149 20 amplicons amplicon NNS work_infoczas7nfbzac5mhjnxpkxle 149 21 , , , work_infoczas7nfbzac5mhjnxpkxle 149 22 all all DT work_infoczas7nfbzac5mhjnxpkxle 149 23 of of IN work_infoczas7nfbzac5mhjnxpkxle 149 24 which which WDT work_infoczas7nfbzac5mhjnxpkxle 149 25 were be VBD work_infoczas7nfbzac5mhjnxpkxle 149 26 found find VBN work_infoczas7nfbzac5mhjnxpkxle 149 27 by by IN work_infoczas7nfbzac5mhjnxpkxle 149 28 sequence sequence NN work_infoczas7nfbzac5mhjnxpkxle 149 29 analysis analysis NN work_infoczas7nfbzac5mhjnxpkxle 149 30 to to TO work_infoczas7nfbzac5mhjnxpkxle 149 31 carry carry VB work_infoczas7nfbzac5mhjnxpkxle 149 32 the the DT work_infoczas7nfbzac5mhjnxpkxle 149 33 hallmarks hallmark NNS work_infoczas7nfbzac5mhjnxpkxle 149 34 of of IN work_infoczas7nfbzac5mhjnxpkxle 149 35 a a DT work_infoczas7nfbzac5mhjnxpkxle 149 36 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 149 37 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 149 38 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 149 39 . . . work_infoczas7nfbzac5mhjnxpkxle 150 1 Four four CD work_infoczas7nfbzac5mhjnxpkxle 150 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 150 3 these these DT work_infoczas7nfbzac5mhjnxpkxle 150 4 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 150 5 were be VBD work_infoczas7nfbzac5mhjnxpkxle 150 6 unique unique JJ work_infoczas7nfbzac5mhjnxpkxle 150 7 and and CC work_infoczas7nfbzac5mhjnxpkxle 150 8 could could MD work_infoczas7nfbzac5mhjnxpkxle 150 9 be be VB work_infoczas7nfbzac5mhjnxpkxle 150 10 mapped map VBN work_infoczas7nfbzac5mhjnxpkxle 150 11 to to IN work_infoczas7nfbzac5mhjnxpkxle 150 12 different different JJ work_infoczas7nfbzac5mhjnxpkxle 150 13 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 150 14 loci loci NN work_infoczas7nfbzac5mhjnxpkxle 150 15 , , , work_infoczas7nfbzac5mhjnxpkxle 150 16 all all DT work_infoczas7nfbzac5mhjnxpkxle 150 17 situated situate VBN work_infoczas7nfbzac5mhjnxpkxle 150 18 in in IN work_infoczas7nfbzac5mhjnxpkxle 150 19 intergenic intergenic JJ work_infoczas7nfbzac5mhjnxpkxle 150 20 regions region NNS work_infoczas7nfbzac5mhjnxpkxle 150 21 9 9 CD work_infoczas7nfbzac5mhjnxpkxle 150 22 of of IN work_infoczas7nfbzac5mhjnxpkxle 150 23 the the DT work_infoczas7nfbzac5mhjnxpkxle 150 24 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 150 25 genome genome NN work_infoczas7nfbzac5mhjnxpkxle 150 26 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 150 27 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 150 28 . . . work_infoczas7nfbzac5mhjnxpkxle 151 1 3 3 LS work_infoczas7nfbzac5mhjnxpkxle 151 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 151 3 . . . work_infoczas7nfbzac5mhjnxpkxle 152 1 In in IN work_infoczas7nfbzac5mhjnxpkxle 152 2 summary summary NN work_infoczas7nfbzac5mhjnxpkxle 152 3 , , , work_infoczas7nfbzac5mhjnxpkxle 152 4 our -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 152 5 genetic genetic JJ work_infoczas7nfbzac5mhjnxpkxle 152 6 analyses analysis NNS work_infoczas7nfbzac5mhjnxpkxle 152 7 show show VBP work_infoczas7nfbzac5mhjnxpkxle 152 8 that that IN work_infoczas7nfbzac5mhjnxpkxle 152 9 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 152 10 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 152 11 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 152 12 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 152 13 was be VBD work_infoczas7nfbzac5mhjnxpkxle 152 14 achieved achieve VBN work_infoczas7nfbzac5mhjnxpkxle 152 15 by by IN work_infoczas7nfbzac5mhjnxpkxle 152 16 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 152 17 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 152 18 into into IN work_infoczas7nfbzac5mhjnxpkxle 152 19 intergenic intergenic JJ work_infoczas7nfbzac5mhjnxpkxle 152 20 regions region NNS work_infoczas7nfbzac5mhjnxpkxle 152 21 . . . work_infoczas7nfbzac5mhjnxpkxle 153 1 Systemic systemic JJ work_infoczas7nfbzac5mhjnxpkxle 153 2 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 153 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 153 4 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 153 5 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 153 6 in in IN work_infoczas7nfbzac5mhjnxpkxle 153 7 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 153 8 harboring harbor VBG work_infoczas7nfbzac5mhjnxpkxle 153 9 multiple multiple JJ work_infoczas7nfbzac5mhjnxpkxle 153 10 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 153 11 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 153 12 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 153 13 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 153 14 We -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 153 15 sacrificed sacrifice VBD work_infoczas7nfbzac5mhjnxpkxle 153 16 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 153 17 # # NN work_infoczas7nfbzac5mhjnxpkxle 153 18 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 153 19 to to TO work_infoczas7nfbzac5mhjnxpkxle 153 20 carry carry VB work_infoczas7nfbzac5mhjnxpkxle 153 21 out out RP work_infoczas7nfbzac5mhjnxpkxle 153 22 careful careful JJ work_infoczas7nfbzac5mhjnxpkxle 153 23 fluorescence fluorescence NN work_infoczas7nfbzac5mhjnxpkxle 153 24 imaging imaging NN work_infoczas7nfbzac5mhjnxpkxle 153 25 of of IN work_infoczas7nfbzac5mhjnxpkxle 153 26 the the DT work_infoczas7nfbzac5mhjnxpkxle 153 27 animal animal NN work_infoczas7nfbzac5mhjnxpkxle 153 28 in in IN work_infoczas7nfbzac5mhjnxpkxle 153 29 comparison comparison NN work_infoczas7nfbzac5mhjnxpkxle 153 30 with with IN work_infoczas7nfbzac5mhjnxpkxle 153 31 an an DT work_infoczas7nfbzac5mhjnxpkxle 153 32 age- age- RB work_infoczas7nfbzac5mhjnxpkxle 153 33 matched match VBN work_infoczas7nfbzac5mhjnxpkxle 153 34 animal animal NN work_infoczas7nfbzac5mhjnxpkxle 153 35 as as IN work_infoczas7nfbzac5mhjnxpkxle 153 36 negative negative JJ work_infoczas7nfbzac5mhjnxpkxle 153 37 control control NN work_infoczas7nfbzac5mhjnxpkxle 153 38 . . . work_infoczas7nfbzac5mhjnxpkxle 154 1 As as IN work_infoczas7nfbzac5mhjnxpkxle 154 2 shown show VBN work_infoczas7nfbzac5mhjnxpkxle 154 3 in in IN work_infoczas7nfbzac5mhjnxpkxle 154 4 Fig Fig NNP work_infoczas7nfbzac5mhjnxpkxle 154 5 . . . work_infoczas7nfbzac5mhjnxpkxle 155 1 4A 4A NNP work_infoczas7nfbzac5mhjnxpkxle 155 2 through through IN work_infoczas7nfbzac5mhjnxpkxle 155 3 Q Q NNP work_infoczas7nfbzac5mhjnxpkxle 155 4 , , , work_infoczas7nfbzac5mhjnxpkxle 155 5 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 155 6 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 155 7 was be VBD work_infoczas7nfbzac5mhjnxpkxle 155 8 detected detect VBN work_infoczas7nfbzac5mhjnxpkxle 155 9 in in IN work_infoczas7nfbzac5mhjnxpkxle 155 10 all all DT work_infoczas7nfbzac5mhjnxpkxle 155 11 seventeen seventeen CD work_infoczas7nfbzac5mhjnxpkxle 155 12 analyzed analyzed JJ work_infoczas7nfbzac5mhjnxpkxle 155 13 tissues tissue NNS work_infoczas7nfbzac5mhjnxpkxle 155 14 of of IN work_infoczas7nfbzac5mhjnxpkxle 155 15 the the DT work_infoczas7nfbzac5mhjnxpkxle 155 16 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 155 17 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 155 18 including include VBG work_infoczas7nfbzac5mhjnxpkxle 155 19 brain brain NN work_infoczas7nfbzac5mhjnxpkxle 155 20 , , , work_infoczas7nfbzac5mhjnxpkxle 155 21 eye eye NN work_infoczas7nfbzac5mhjnxpkxle 155 22 , , , work_infoczas7nfbzac5mhjnxpkxle 155 23 skeletal skeletal JJ work_infoczas7nfbzac5mhjnxpkxle 155 24 muscle muscle NN work_infoczas7nfbzac5mhjnxpkxle 155 25 , , , work_infoczas7nfbzac5mhjnxpkxle 155 26 heart heart NN work_infoczas7nfbzac5mhjnxpkxle 155 27 , , , work_infoczas7nfbzac5mhjnxpkxle 155 28 lung lung NN work_infoczas7nfbzac5mhjnxpkxle 155 29 , , , work_infoczas7nfbzac5mhjnxpkxle 155 30 liver liver NN work_infoczas7nfbzac5mhjnxpkxle 155 31 , , , work_infoczas7nfbzac5mhjnxpkxle 155 32 and and CC work_infoczas7nfbzac5mhjnxpkxle 155 33 skin skin NN work_infoczas7nfbzac5mhjnxpkxle 155 34 . . . work_infoczas7nfbzac5mhjnxpkxle 156 1 Some some DT work_infoczas7nfbzac5mhjnxpkxle 156 2 autofluorescence autofluorescence NN work_infoczas7nfbzac5mhjnxpkxle 156 3 could could MD work_infoczas7nfbzac5mhjnxpkxle 156 4 be be VB work_infoczas7nfbzac5mhjnxpkxle 156 5 detected detect VBN work_infoczas7nfbzac5mhjnxpkxle 156 6 in in IN work_infoczas7nfbzac5mhjnxpkxle 156 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 156 8 liver liver NN work_infoczas7nfbzac5mhjnxpkxle 156 9 , , , work_infoczas7nfbzac5mhjnxpkxle 156 10 heart heart NN work_infoczas7nfbzac5mhjnxpkxle 156 11 , , , work_infoczas7nfbzac5mhjnxpkxle 156 12 and and CC work_infoczas7nfbzac5mhjnxpkxle 156 13 rib rib NN work_infoczas7nfbzac5mhjnxpkxle 156 14 bone bone NN work_infoczas7nfbzac5mhjnxpkxle 156 15 of of IN work_infoczas7nfbzac5mhjnxpkxle 156 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 156 17 control control NN work_infoczas7nfbzac5mhjnxpkxle 156 18 pig pig NNP work_infoczas7nfbzac5mhjnxpkxle 156 19 but but CC work_infoczas7nfbzac5mhjnxpkxle 156 20 fluorescence fluorescence NN work_infoczas7nfbzac5mhjnxpkxle 156 21 was be VBD work_infoczas7nfbzac5mhjnxpkxle 156 22 still still RB work_infoczas7nfbzac5mhjnxpkxle 156 23 considerably considerably RB work_infoczas7nfbzac5mhjnxpkxle 156 24 stronger strong JJR work_infoczas7nfbzac5mhjnxpkxle 156 25 in in IN work_infoczas7nfbzac5mhjnxpkxle 156 26 the the DT work_infoczas7nfbzac5mhjnxpkxle 156 27 tissues tissue NNS work_infoczas7nfbzac5mhjnxpkxle 156 28 from from IN work_infoczas7nfbzac5mhjnxpkxle 156 29 the the DT work_infoczas7nfbzac5mhjnxpkxle 156 30 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 156 31 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 156 32 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 156 33 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 156 34 . . . work_infoczas7nfbzac5mhjnxpkxle 157 1 4 4 LS work_infoczas7nfbzac5mhjnxpkxle 157 2 , , , work_infoczas7nfbzac5mhjnxpkxle 157 3 panels panel NNS work_infoczas7nfbzac5mhjnxpkxle 157 4 B1 B1 NNP work_infoczas7nfbzac5mhjnxpkxle 157 5 / / SYM work_infoczas7nfbzac5mhjnxpkxle 157 6 B2 b2 NN work_infoczas7nfbzac5mhjnxpkxle 157 7 , , , work_infoczas7nfbzac5mhjnxpkxle 157 8 G1 G1 NNP work_infoczas7nfbzac5mhjnxpkxle 157 9 / / SYM work_infoczas7nfbzac5mhjnxpkxle 157 10 G2 G2 NNP work_infoczas7nfbzac5mhjnxpkxle 157 11 , , , work_infoczas7nfbzac5mhjnxpkxle 157 12 and and CC work_infoczas7nfbzac5mhjnxpkxle 157 13 P1 P1 NNP work_infoczas7nfbzac5mhjnxpkxle 157 14 / / SYM work_infoczas7nfbzac5mhjnxpkxle 157 15 P2 P2 NNP work_infoczas7nfbzac5mhjnxpkxle 157 16 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 157 17 . . . work_infoczas7nfbzac5mhjnxpkxle 158 1 Heart heart NN work_infoczas7nfbzac5mhjnxpkxle 158 2 and and CC work_infoczas7nfbzac5mhjnxpkxle 158 3 testis testis VB work_infoczas7nfbzac5mhjnxpkxle 158 4 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 158 5 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 158 6 . . . work_infoczas7nfbzac5mhjnxpkxle 159 1 4 4 LS work_infoczas7nfbzac5mhjnxpkxle 159 2 , , , work_infoczas7nfbzac5mhjnxpkxle 159 3 panels panel VBZ work_infoczas7nfbzac5mhjnxpkxle 159 4 G2 G2 NNP work_infoczas7nfbzac5mhjnxpkxle 159 5 and and CC work_infoczas7nfbzac5mhjnxpkxle 159 6 H2 H2 NNP work_infoczas7nfbzac5mhjnxpkxle 159 7 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 159 8 did do VBD work_infoczas7nfbzac5mhjnxpkxle 159 9 not not RB work_infoczas7nfbzac5mhjnxpkxle 159 10 show show VB work_infoczas7nfbzac5mhjnxpkxle 159 11 similar similar JJ work_infoczas7nfbzac5mhjnxpkxle 159 12 uniform uniform JJ work_infoczas7nfbzac5mhjnxpkxle 159 13 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 159 14 of of IN work_infoczas7nfbzac5mhjnxpkxle 159 15 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 159 16 compared compare VBN work_infoczas7nfbzac5mhjnxpkxle 159 17 to to IN work_infoczas7nfbzac5mhjnxpkxle 159 18 the the DT work_infoczas7nfbzac5mhjnxpkxle 159 19 remaining remain VBG work_infoczas7nfbzac5mhjnxpkxle 159 20 organs organ NNS work_infoczas7nfbzac5mhjnxpkxle 159 21 , , , work_infoczas7nfbzac5mhjnxpkxle 159 22 and and CC work_infoczas7nfbzac5mhjnxpkxle 159 23 the the DT work_infoczas7nfbzac5mhjnxpkxle 159 24 fat fat JJ work_infoczas7nfbzac5mhjnxpkxle 159 25 layer layer NN work_infoczas7nfbzac5mhjnxpkxle 159 26 covering cover VBG work_infoczas7nfbzac5mhjnxpkxle 159 27 the the DT work_infoczas7nfbzac5mhjnxpkxle 159 28 heart heart NN work_infoczas7nfbzac5mhjnxpkxle 159 29 had have VBD work_infoczas7nfbzac5mhjnxpkxle 159 30 increased increase VBN work_infoczas7nfbzac5mhjnxpkxle 159 31 fluorescence fluorescence NN work_infoczas7nfbzac5mhjnxpkxle 159 32 compared compare VBN work_infoczas7nfbzac5mhjnxpkxle 159 33 to to IN work_infoczas7nfbzac5mhjnxpkxle 159 34 the the DT work_infoczas7nfbzac5mhjnxpkxle 159 35 smooth smooth JJ work_infoczas7nfbzac5mhjnxpkxle 159 36 heart heart NN work_infoczas7nfbzac5mhjnxpkxle 159 37 muscle muscle NN work_infoczas7nfbzac5mhjnxpkxle 159 38 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 159 39 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 159 40 . . . work_infoczas7nfbzac5mhjnxpkxle 160 1 4 4 LS work_infoczas7nfbzac5mhjnxpkxle 160 2 , , , work_infoczas7nfbzac5mhjnxpkxle 160 3 panels panel VBZ work_infoczas7nfbzac5mhjnxpkxle 160 4 G1 G1 NNP work_infoczas7nfbzac5mhjnxpkxle 160 5 and and CC work_infoczas7nfbzac5mhjnxpkxle 160 6 G2 G2 NNP work_infoczas7nfbzac5mhjnxpkxle 160 7 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 160 8 , , , work_infoczas7nfbzac5mhjnxpkxle 160 9 whereas whereas IN work_infoczas7nfbzac5mhjnxpkxle 160 10 fluorescence fluorescence NN work_infoczas7nfbzac5mhjnxpkxle 160 11 in in IN work_infoczas7nfbzac5mhjnxpkxle 160 12 the the DT work_infoczas7nfbzac5mhjnxpkxle 160 13 epididymis epididymis NNP work_infoczas7nfbzac5mhjnxpkxle 160 14 was be VBD work_infoczas7nfbzac5mhjnxpkxle 160 15 considerably considerably RB work_infoczas7nfbzac5mhjnxpkxle 160 16 higher high JJR work_infoczas7nfbzac5mhjnxpkxle 160 17 than than IN work_infoczas7nfbzac5mhjnxpkxle 160 18 in in IN work_infoczas7nfbzac5mhjnxpkxle 160 19 the the DT work_infoczas7nfbzac5mhjnxpkxle 160 20 testis testis NN work_infoczas7nfbzac5mhjnxpkxle 160 21 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 160 22 Fig fig NN work_infoczas7nfbzac5mhjnxpkxle 160 23 . . . work_infoczas7nfbzac5mhjnxpkxle 161 1 4 4 LS work_infoczas7nfbzac5mhjnxpkxle 161 2 , , , work_infoczas7nfbzac5mhjnxpkxle 161 3 panels panel VBZ work_infoczas7nfbzac5mhjnxpkxle 161 4 H1 H1 NNP work_infoczas7nfbzac5mhjnxpkxle 161 5 and and CC work_infoczas7nfbzac5mhjnxpkxle 161 6 H2 H2 NNP work_infoczas7nfbzac5mhjnxpkxle 161 7 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 161 8 . . . work_infoczas7nfbzac5mhjnxpkxle 162 1 This this DT work_infoczas7nfbzac5mhjnxpkxle 162 2 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 162 3 contained contain VBD work_infoczas7nfbzac5mhjnxpkxle 162 4 a a DT work_infoczas7nfbzac5mhjnxpkxle 162 5 minimum minimum NN work_infoczas7nfbzac5mhjnxpkxle 162 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 162 7 four four CD work_infoczas7nfbzac5mhjnxpkxle 162 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 162 9 possibly possibly RB work_infoczas7nfbzac5mhjnxpkxle 162 10 more more JJR work_infoczas7nfbzac5mhjnxpkxle 162 11 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 162 12 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 162 13 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 162 14 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 162 15 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 162 16 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 162 17 flanked flank VBN work_infoczas7nfbzac5mhjnxpkxle 162 18 by by IN work_infoczas7nfbzac5mhjnxpkxle 162 19 cHS4 cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 162 20 insulators insulator NNS work_infoczas7nfbzac5mhjnxpkxle 162 21 . . . work_infoczas7nfbzac5mhjnxpkxle 163 1 It -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 163 2 was be VBD work_infoczas7nfbzac5mhjnxpkxle 163 3 not not RB work_infoczas7nfbzac5mhjnxpkxle 163 4 possible possible JJ work_infoczas7nfbzac5mhjnxpkxle 163 5 at at IN work_infoczas7nfbzac5mhjnxpkxle 163 6 this this DT work_infoczas7nfbzac5mhjnxpkxle 163 7 stage stage NN work_infoczas7nfbzac5mhjnxpkxle 163 8 to to TO work_infoczas7nfbzac5mhjnxpkxle 163 9 determine determine VB work_infoczas7nfbzac5mhjnxpkxle 163 10 the the DT work_infoczas7nfbzac5mhjnxpkxle 163 11 contribution contribution NN work_infoczas7nfbzac5mhjnxpkxle 163 12 of of IN work_infoczas7nfbzac5mhjnxpkxle 163 13 each each DT work_infoczas7nfbzac5mhjnxpkxle 163 14 of of IN work_infoczas7nfbzac5mhjnxpkxle 163 15 the the DT work_infoczas7nfbzac5mhjnxpkxle 163 16 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 163 17 to to IN work_infoczas7nfbzac5mhjnxpkxle 163 18 the the DT work_infoczas7nfbzac5mhjnxpkxle 163 19 overall overall JJ work_infoczas7nfbzac5mhjnxpkxle 163 20 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 163 21 of of IN work_infoczas7nfbzac5mhjnxpkxle 163 22 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 163 23 . . . work_infoczas7nfbzac5mhjnxpkxle 164 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 164 2 animals animal NNS work_infoczas7nfbzac5mhjnxpkxle 164 3 are be VBP work_infoczas7nfbzac5mhjnxpkxle 164 4 currently currently RB work_infoczas7nfbzac5mhjnxpkxle 164 5 being be VBG work_infoczas7nfbzac5mhjnxpkxle 164 6 bred breed VBN work_infoczas7nfbzac5mhjnxpkxle 164 7 and and CC work_infoczas7nfbzac5mhjnxpkxle 164 8 future future JJ work_infoczas7nfbzac5mhjnxpkxle 164 9 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 164 10 offspring offspring NN work_infoczas7nfbzac5mhjnxpkxle 164 11 will will MD work_infoczas7nfbzac5mhjnxpkxle 164 12 be be VB work_infoczas7nfbzac5mhjnxpkxle 164 13 analyzed analyze VBN work_infoczas7nfbzac5mhjnxpkxle 164 14 for for IN work_infoczas7nfbzac5mhjnxpkxle 164 15 persistency persistency NN work_infoczas7nfbzac5mhjnxpkxle 164 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 164 17 and and CC work_infoczas7nfbzac5mhjnxpkxle 164 18 differences difference NNS work_infoczas7nfbzac5mhjnxpkxle 164 19 in in IN work_infoczas7nfbzac5mhjnxpkxle 164 20 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 164 21 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 164 22 . . . work_infoczas7nfbzac5mhjnxpkxle 165 1 10 10 CD work_infoczas7nfbzac5mhjnxpkxle 165 2 DISCUSSION discussion NN work_infoczas7nfbzac5mhjnxpkxle 165 3 We -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 165 4 describe describe VBP work_infoczas7nfbzac5mhjnxpkxle 165 5 here here RB work_infoczas7nfbzac5mhjnxpkxle 165 6 the the DT work_infoczas7nfbzac5mhjnxpkxle 165 7 production production NN work_infoczas7nfbzac5mhjnxpkxle 165 8 of of IN work_infoczas7nfbzac5mhjnxpkxle 165 9 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 165 10 cloned clone VBN work_infoczas7nfbzac5mhjnxpkxle 165 11 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 165 12 by by IN work_infoczas7nfbzac5mhjnxpkxle 165 13 HMC HMC NNP work_infoczas7nfbzac5mhjnxpkxle 165 14 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 165 15 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 165 16 nuclear nuclear JJ work_infoczas7nfbzac5mhjnxpkxle 165 17 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 165 18 using use VBG work_infoczas7nfbzac5mhjnxpkxle 165 19 primary primary JJ work_infoczas7nfbzac5mhjnxpkxle 165 20 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 165 21 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 165 22 modified modify VBN work_infoczas7nfbzac5mhjnxpkxle 165 23 by by IN work_infoczas7nfbzac5mhjnxpkxle 165 24 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 165 25 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 165 26 of of IN work_infoczas7nfbzac5mhjnxpkxle 165 27 a a DT work_infoczas7nfbzac5mhjnxpkxle 165 28 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 165 29 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 165 30 tagged tag VBN work_infoczas7nfbzac5mhjnxpkxle 165 31 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 165 32 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 165 33 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 165 34 transposon transposon JJ work_infoczas7nfbzac5mhjnxpkxle 165 35 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 165 36 . . . work_infoczas7nfbzac5mhjnxpkxle 166 1 By by IN work_infoczas7nfbzac5mhjnxpkxle 166 2 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 166 3 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 166 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 166 5 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 166 6 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 166 7 of of IN work_infoczas7nfbzac5mhjnxpkxle 166 8 the the DT work_infoczas7nfbzac5mhjnxpkxle 166 9 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 166 10 marker marker NN work_infoczas7nfbzac5mhjnxpkxle 166 11 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 166 12 , , , work_infoczas7nfbzac5mhjnxpkxle 166 13 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 166 14 established establish VBD work_infoczas7nfbzac5mhjnxpkxle 166 15 Göttingen Göttingen NNP work_infoczas7nfbzac5mhjnxpkxle 166 16 minipigs minipig NNS work_infoczas7nfbzac5mhjnxpkxle 166 17 with with IN work_infoczas7nfbzac5mhjnxpkxle 166 18 systemic systemic JJ work_infoczas7nfbzac5mhjnxpkxle 166 19 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 166 20 of of IN work_infoczas7nfbzac5mhjnxpkxle 166 21 the the DT work_infoczas7nfbzac5mhjnxpkxle 166 22 transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 166 23 . . . work_infoczas7nfbzac5mhjnxpkxle 167 1 Hence hence RB work_infoczas7nfbzac5mhjnxpkxle 167 2 , , , work_infoczas7nfbzac5mhjnxpkxle 167 3 all all DT work_infoczas7nfbzac5mhjnxpkxle 167 4 of of IN work_infoczas7nfbzac5mhjnxpkxle 167 5 seventeen seventeen CD work_infoczas7nfbzac5mhjnxpkxle 167 6 analyzed analyzed JJ work_infoczas7nfbzac5mhjnxpkxle 167 7 tissues tissue NNS work_infoczas7nfbzac5mhjnxpkxle 167 8 , , , work_infoczas7nfbzac5mhjnxpkxle 167 9 including include VBG work_infoczas7nfbzac5mhjnxpkxle 167 10 brain brain NN work_infoczas7nfbzac5mhjnxpkxle 167 11 , , , work_infoczas7nfbzac5mhjnxpkxle 167 12 eye eye NN work_infoczas7nfbzac5mhjnxpkxle 167 13 , , , work_infoczas7nfbzac5mhjnxpkxle 167 14 muscle muscle NN work_infoczas7nfbzac5mhjnxpkxle 167 15 , , , work_infoczas7nfbzac5mhjnxpkxle 167 16 heart heart NN work_infoczas7nfbzac5mhjnxpkxle 167 17 , , , work_infoczas7nfbzac5mhjnxpkxle 167 18 lung lung NN work_infoczas7nfbzac5mhjnxpkxle 167 19 , , , work_infoczas7nfbzac5mhjnxpkxle 167 20 liver liver NN work_infoczas7nfbzac5mhjnxpkxle 167 21 , , , work_infoczas7nfbzac5mhjnxpkxle 167 22 and and CC work_infoczas7nfbzac5mhjnxpkxle 167 23 skin skin NN work_infoczas7nfbzac5mhjnxpkxle 167 24 , , , work_infoczas7nfbzac5mhjnxpkxle 167 25 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 167 26 from from IN work_infoczas7nfbzac5mhjnxpkxle 167 27 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 167 28 containing contain VBG work_infoczas7nfbzac5mhjnxpkxle 167 29 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 167 30 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 167 31 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 167 32 were be VBD work_infoczas7nfbzac5mhjnxpkxle 167 33 found find VBN work_infoczas7nfbzac5mhjnxpkxle 167 34 to to TO work_infoczas7nfbzac5mhjnxpkxle 167 35 express express VB work_infoczas7nfbzac5mhjnxpkxle 167 36 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 167 37 by by IN work_infoczas7nfbzac5mhjnxpkxle 167 38 fluorescence fluorescence NN work_infoczas7nfbzac5mhjnxpkxle 167 39 imaging imaging NN work_infoczas7nfbzac5mhjnxpkxle 167 40 . . . work_infoczas7nfbzac5mhjnxpkxle 168 1 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 168 2 facilitates facilitate VBZ work_infoczas7nfbzac5mhjnxpkxle 168 3 efficient efficient JJ work_infoczas7nfbzac5mhjnxpkxle 168 4 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 168 5 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 168 6 in in IN work_infoczas7nfbzac5mhjnxpkxle 168 7 a a DT work_infoczas7nfbzac5mhjnxpkxle 168 8 wide wide JJ work_infoczas7nfbzac5mhjnxpkxle 168 9 panel panel NN work_infoczas7nfbzac5mhjnxpkxle 168 10 of of IN work_infoczas7nfbzac5mhjnxpkxle 168 11 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 168 12 types type NNS work_infoczas7nfbzac5mhjnxpkxle 168 13 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 168 14 Izsvak Izsvak NNP work_infoczas7nfbzac5mhjnxpkxle 168 15 et et FW work_infoczas7nfbzac5mhjnxpkxle 168 16 al al NNP work_infoczas7nfbzac5mhjnxpkxle 168 17 . . . work_infoczas7nfbzac5mhjnxpkxle 169 1 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 169 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 169 3 and and CC work_infoczas7nfbzac5mhjnxpkxle 169 4 represents represent VBZ work_infoczas7nfbzac5mhjnxpkxle 169 5 an an DT work_infoczas7nfbzac5mhjnxpkxle 169 6 attractive attractive JJ work_infoczas7nfbzac5mhjnxpkxle 169 7 non non JJ work_infoczas7nfbzac5mhjnxpkxle 169 8 - - JJ work_infoczas7nfbzac5mhjnxpkxle 169 9 viral viral JJ work_infoczas7nfbzac5mhjnxpkxle 169 10 approach approach NN work_infoczas7nfbzac5mhjnxpkxle 169 11 for for IN work_infoczas7nfbzac5mhjnxpkxle 169 12 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 169 13 delivery delivery NN work_infoczas7nfbzac5mhjnxpkxle 169 14 and and CC work_infoczas7nfbzac5mhjnxpkxle 169 15 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 169 16 in in IN work_infoczas7nfbzac5mhjnxpkxle 169 17 both both DT work_infoczas7nfbzac5mhjnxpkxle 169 18 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 169 19 therapy therapy NN work_infoczas7nfbzac5mhjnxpkxle 169 20 applications application NNS work_infoczas7nfbzac5mhjnxpkxle 169 21 and and CC work_infoczas7nfbzac5mhjnxpkxle 169 22 animal animal NN work_infoczas7nfbzac5mhjnxpkxle 169 23 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 169 24 . . . work_infoczas7nfbzac5mhjnxpkxle 170 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 170 2 level level NN work_infoczas7nfbzac5mhjnxpkxle 170 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 170 4 stable stable JJ work_infoczas7nfbzac5mhjnxpkxle 170 5 transfection transfection NN work_infoczas7nfbzac5mhjnxpkxle 170 6 mediated mediate VBN work_infoczas7nfbzac5mhjnxpkxle 170 7 by by IN work_infoczas7nfbzac5mhjnxpkxle 170 8 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 170 9 in in IN work_infoczas7nfbzac5mhjnxpkxle 170 10 a a DT work_infoczas7nfbzac5mhjnxpkxle 170 11 particular particular JJ work_infoczas7nfbzac5mhjnxpkxle 170 12 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 170 13 type type NN work_infoczas7nfbzac5mhjnxpkxle 170 14 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 170 15 under under IN work_infoczas7nfbzac5mhjnxpkxle 170 16 influence influence NN work_infoczas7nfbzac5mhjnxpkxle 170 17 of of IN work_infoczas7nfbzac5mhjnxpkxle 170 18 the the DT work_infoczas7nfbzac5mhjnxpkxle 170 19 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 170 20 transfection transfection NN work_infoczas7nfbzac5mhjnxpkxle 170 21 efficiency efficiency NN work_infoczas7nfbzac5mhjnxpkxle 170 22 as as RB work_infoczas7nfbzac5mhjnxpkxle 170 23 well well RB work_infoczas7nfbzac5mhjnxpkxle 170 24 as as IN work_infoczas7nfbzac5mhjnxpkxle 170 25 the the DT work_infoczas7nfbzac5mhjnxpkxle 170 26 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 170 27 mobilization mobilization NN work_infoczas7nfbzac5mhjnxpkxle 170 28 rate rate NN work_infoczas7nfbzac5mhjnxpkxle 170 29 , , , work_infoczas7nfbzac5mhjnxpkxle 170 30 both both DT work_infoczas7nfbzac5mhjnxpkxle 170 31 of of IN work_infoczas7nfbzac5mhjnxpkxle 170 32 which which WDT work_infoczas7nfbzac5mhjnxpkxle 170 33 may may MD work_infoczas7nfbzac5mhjnxpkxle 170 34 vary vary VB work_infoczas7nfbzac5mhjnxpkxle 170 35 considerably considerably RB work_infoczas7nfbzac5mhjnxpkxle 170 36 between between IN work_infoczas7nfbzac5mhjnxpkxle 170 37 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 170 38 types type NNS work_infoczas7nfbzac5mhjnxpkxle 170 39 . . . work_infoczas7nfbzac5mhjnxpkxle 171 1 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 171 2 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 171 3 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 171 4 supported support VBN work_infoczas7nfbzac5mhjnxpkxle 171 5 by by IN work_infoczas7nfbzac5mhjnxpkxle 171 6 cellular cellular JJ work_infoczas7nfbzac5mhjnxpkxle 171 7 factors factor NNS work_infoczas7nfbzac5mhjnxpkxle 171 8 like like IN work_infoczas7nfbzac5mhjnxpkxle 171 9 the the DT work_infoczas7nfbzac5mhjnxpkxle 171 10 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 171 11 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 171 12 bending bend VBG work_infoczas7nfbzac5mhjnxpkxle 171 13 protein protein NN work_infoczas7nfbzac5mhjnxpkxle 171 14 HMGB1 HMGB1 NNS work_infoczas7nfbzac5mhjnxpkxle 171 15 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 171 16 Zayed Zayed NNP work_infoczas7nfbzac5mhjnxpkxle 171 17 et et NNP work_infoczas7nfbzac5mhjnxpkxle 171 18 al al NNP work_infoczas7nfbzac5mhjnxpkxle 171 19 . . . work_infoczas7nfbzac5mhjnxpkxle 172 1 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 172 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 172 3 and and CC work_infoczas7nfbzac5mhjnxpkxle 172 4 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 172 5 influenced influence VBN work_infoczas7nfbzac5mhjnxpkxle 172 6 by by IN work_infoczas7nfbzac5mhjnxpkxle 172 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 172 8 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 172 9 concentration concentration NN work_infoczas7nfbzac5mhjnxpkxle 172 10 , , , work_infoczas7nfbzac5mhjnxpkxle 172 11 which which WDT work_infoczas7nfbzac5mhjnxpkxle 172 12 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 172 13 likely likely JJ work_infoczas7nfbzac5mhjnxpkxle 172 14 to to TO work_infoczas7nfbzac5mhjnxpkxle 172 15 vary vary VB work_infoczas7nfbzac5mhjnxpkxle 172 16 between between IN work_infoczas7nfbzac5mhjnxpkxle 172 17 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 172 18 types type NNS work_infoczas7nfbzac5mhjnxpkxle 172 19 depending depend VBG work_infoczas7nfbzac5mhjnxpkxle 172 20 on on IN work_infoczas7nfbzac5mhjnxpkxle 172 21 efficacy efficacy NN work_infoczas7nfbzac5mhjnxpkxle 172 22 of of IN work_infoczas7nfbzac5mhjnxpkxle 172 23 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 172 24 uptake uptake JJ work_infoczas7nfbzac5mhjnxpkxle 172 25 and and CC work_infoczas7nfbzac5mhjnxpkxle 172 26 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 172 27 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 172 28 design design NN work_infoczas7nfbzac5mhjnxpkxle 172 29 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 172 30 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 172 31 et et NNP work_infoczas7nfbzac5mhjnxpkxle 172 32 al al NNP work_infoczas7nfbzac5mhjnxpkxle 172 33 . . . work_infoczas7nfbzac5mhjnxpkxle 173 1 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 173 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 173 3 . . . work_infoczas7nfbzac5mhjnxpkxle 174 1 In in IN work_infoczas7nfbzac5mhjnxpkxle 174 2 NPFs NPFs NNP work_infoczas7nfbzac5mhjnxpkxle 174 3 , , , work_infoczas7nfbzac5mhjnxpkxle 174 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 174 5 level level NN work_infoczas7nfbzac5mhjnxpkxle 174 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 174 7 stable stable JJ work_infoczas7nfbzac5mhjnxpkxle 174 8 transfection transfection NN work_infoczas7nfbzac5mhjnxpkxle 174 9 obtained obtain VBN work_infoczas7nfbzac5mhjnxpkxle 174 10 with with IN work_infoczas7nfbzac5mhjnxpkxle 174 11 the the DT work_infoczas7nfbzac5mhjnxpkxle 174 12 HSB3 HSB3 NNP work_infoczas7nfbzac5mhjnxpkxle 174 13 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 174 14 was be VBD work_infoczas7nfbzac5mhjnxpkxle 174 15 significantly significantly RB work_infoczas7nfbzac5mhjnxpkxle 174 16 increased increase VBN work_infoczas7nfbzac5mhjnxpkxle 174 17 over over IN work_infoczas7nfbzac5mhjnxpkxle 174 18 background background NN work_infoczas7nfbzac5mhjnxpkxle 174 19 but but CC work_infoczas7nfbzac5mhjnxpkxle 174 20 was be VBD work_infoczas7nfbzac5mhjnxpkxle 174 21 relatively relatively RB work_infoczas7nfbzac5mhjnxpkxle 174 22 low low JJ work_infoczas7nfbzac5mhjnxpkxle 174 23 compared compare VBN work_infoczas7nfbzac5mhjnxpkxle 174 24 to to IN work_infoczas7nfbzac5mhjnxpkxle 174 25 levels level NNS work_infoczas7nfbzac5mhjnxpkxle 174 26 that that IN work_infoczas7nfbzac5mhjnxpkxle 174 27 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 174 28 routinely routinely RB work_infoczas7nfbzac5mhjnxpkxle 174 29 measure measure VBP work_infoczas7nfbzac5mhjnxpkxle 174 30 in in IN work_infoczas7nfbzac5mhjnxpkxle 174 31 murine murine NNP work_infoczas7nfbzac5mhjnxpkxle 174 32 or or CC work_infoczas7nfbzac5mhjnxpkxle 174 33 human human JJ work_infoczas7nfbzac5mhjnxpkxle 174 34 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 174 35 lines line NNS work_infoczas7nfbzac5mhjnxpkxle 174 36 . . . work_infoczas7nfbzac5mhjnxpkxle 175 1 As as IN work_infoczas7nfbzac5mhjnxpkxle 175 2 the the DT work_infoczas7nfbzac5mhjnxpkxle 175 3 NPFs npf NNS work_infoczas7nfbzac5mhjnxpkxle 175 4 were be VBD work_infoczas7nfbzac5mhjnxpkxle 175 5 quite quite RB work_infoczas7nfbzac5mhjnxpkxle 175 6 efficiently efficiently RB work_infoczas7nfbzac5mhjnxpkxle 175 7 transfected transfecte VBN work_infoczas7nfbzac5mhjnxpkxle 175 8 with with IN work_infoczas7nfbzac5mhjnxpkxle 175 9 at at RB work_infoczas7nfbzac5mhjnxpkxle 175 10 least least RBS work_infoczas7nfbzac5mhjnxpkxle 175 11 50 50 CD work_infoczas7nfbzac5mhjnxpkxle 175 12 % % NN work_infoczas7nfbzac5mhjnxpkxle 175 13 of of IN work_infoczas7nfbzac5mhjnxpkxle 175 14 the the DT work_infoczas7nfbzac5mhjnxpkxle 175 15 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 175 16 transiently transiently RB work_infoczas7nfbzac5mhjnxpkxle 175 17 expressing express VBG work_infoczas7nfbzac5mhjnxpkxle 175 18 the the DT work_infoczas7nfbzac5mhjnxpkxle 175 19 transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 175 20 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 175 21 data datum NNS work_infoczas7nfbzac5mhjnxpkxle 175 22 not not RB work_infoczas7nfbzac5mhjnxpkxle 175 23 shown show VBN work_infoczas7nfbzac5mhjnxpkxle 175 24 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 175 25 , , , work_infoczas7nfbzac5mhjnxpkxle 175 26 our -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 175 27 findings finding NNS work_infoczas7nfbzac5mhjnxpkxle 175 28 most most RBS work_infoczas7nfbzac5mhjnxpkxle 175 29 likely likely RB work_infoczas7nfbzac5mhjnxpkxle 175 30 reflect reflect VBP work_infoczas7nfbzac5mhjnxpkxle 175 31 suboptimal suboptimal JJ work_infoczas7nfbzac5mhjnxpkxle 175 32 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 175 33 rates rate NNS work_infoczas7nfbzac5mhjnxpkxle 175 34 in in IN work_infoczas7nfbzac5mhjnxpkxle 175 35 NPFs NPFs NNP work_infoczas7nfbzac5mhjnxpkxle 175 36 . . . work_infoczas7nfbzac5mhjnxpkxle 176 1 Still still RB work_infoczas7nfbzac5mhjnxpkxle 176 2 , , , work_infoczas7nfbzac5mhjnxpkxle 176 3 the the DT work_infoczas7nfbzac5mhjnxpkxle 176 4 HSB3 HSB3 NNP work_infoczas7nfbzac5mhjnxpkxle 176 5 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 176 6 increased increase VBD work_infoczas7nfbzac5mhjnxpkxle 176 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 176 8 level level NN work_infoczas7nfbzac5mhjnxpkxle 176 9 of of IN work_infoczas7nfbzac5mhjnxpkxle 176 10 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 176 11 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 176 12 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 176 13 as as IN work_infoczas7nfbzac5mhjnxpkxle 176 14 measured measure VBN work_infoczas7nfbzac5mhjnxpkxle 176 15 by by IN work_infoczas7nfbzac5mhjnxpkxle 176 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 176 17 number number NN work_infoczas7nfbzac5mhjnxpkxle 176 18 of of IN work_infoczas7nfbzac5mhjnxpkxle 176 19 drug drug NN work_infoczas7nfbzac5mhjnxpkxle 176 20 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 176 21 resistant resistant JJ work_infoczas7nfbzac5mhjnxpkxle 176 22 colonies colony NNS work_infoczas7nfbzac5mhjnxpkxle 176 23 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 176 24 12-fold 12-fold CD work_infoczas7nfbzac5mhjnxpkxle 176 25 relative relative NN work_infoczas7nfbzac5mhjnxpkxle 176 26 to to IN work_infoczas7nfbzac5mhjnxpkxle 176 27 the the DT work_infoczas7nfbzac5mhjnxpkxle 176 28 negative negative JJ work_infoczas7nfbzac5mhjnxpkxle 176 29 control control NN work_infoczas7nfbzac5mhjnxpkxle 176 30 in in IN work_infoczas7nfbzac5mhjnxpkxle 176 31 which which WDT work_infoczas7nfbzac5mhjnxpkxle 176 32 pUC19 pUC19 NNP work_infoczas7nfbzac5mhjnxpkxle 176 33 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 176 34 was be VBD work_infoczas7nfbzac5mhjnxpkxle 176 35 co co VBN work_infoczas7nfbzac5mhjnxpkxle 176 36 - - VBN work_infoczas7nfbzac5mhjnxpkxle 176 37 transfected transfecte VBN work_infoczas7nfbzac5mhjnxpkxle 176 38 with with IN work_infoczas7nfbzac5mhjnxpkxle 176 39 the the DT work_infoczas7nfbzac5mhjnxpkxle 176 40 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 176 41 donor donor NN work_infoczas7nfbzac5mhjnxpkxle 176 42 plasmid plasmid NNP work_infoczas7nfbzac5mhjnxpkxle 176 43 . . . work_infoczas7nfbzac5mhjnxpkxle 177 1 In in IN work_infoczas7nfbzac5mhjnxpkxle 177 2 analogy analogy NN work_infoczas7nfbzac5mhjnxpkxle 177 3 , , , work_infoczas7nfbzac5mhjnxpkxle 177 4 previous previous JJ work_infoczas7nfbzac5mhjnxpkxle 177 5 work work NN work_infoczas7nfbzac5mhjnxpkxle 177 6 by by IN work_infoczas7nfbzac5mhjnxpkxle 177 7 Clark Clark NNP work_infoczas7nfbzac5mhjnxpkxle 177 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 177 9 co co NNS work_infoczas7nfbzac5mhjnxpkxle 177 10 - - NNS work_infoczas7nfbzac5mhjnxpkxle 177 11 workers worker NNS work_infoczas7nfbzac5mhjnxpkxle 177 12 showed show VBD work_infoczas7nfbzac5mhjnxpkxle 177 13 that that IN work_infoczas7nfbzac5mhjnxpkxle 177 14 the the DT work_infoczas7nfbzac5mhjnxpkxle 177 15 SB11 SB11 NNP work_infoczas7nfbzac5mhjnxpkxle 177 16 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 177 17 variant variant JJ work_infoczas7nfbzac5mhjnxpkxle 177 18 increased increase VBN work_infoczas7nfbzac5mhjnxpkxle 177 19 colony colony NN work_infoczas7nfbzac5mhjnxpkxle 177 20 formation formation NN work_infoczas7nfbzac5mhjnxpkxle 177 21 in in IN work_infoczas7nfbzac5mhjnxpkxle 177 22 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 177 23 fetal fetal JJ work_infoczas7nfbzac5mhjnxpkxle 177 24 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 177 25 2.5-fold 2.5-fold CD work_infoczas7nfbzac5mhjnxpkxle 177 26 over over IN work_infoczas7nfbzac5mhjnxpkxle 177 27 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 177 28 transfected transfecte VBN work_infoczas7nfbzac5mhjnxpkxle 177 29 with with IN work_infoczas7nfbzac5mhjnxpkxle 177 30 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 177 31 encoding encode VBG work_infoczas7nfbzac5mhjnxpkxle 177 32 an an DT work_infoczas7nfbzac5mhjnxpkxle 177 33 inactive inactive JJ work_infoczas7nfbzac5mhjnxpkxle 177 34 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 177 35 variant variant NN work_infoczas7nfbzac5mhjnxpkxle 177 36 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 177 37 Clark Clark NNP work_infoczas7nfbzac5mhjnxpkxle 177 38 et et NNP work_infoczas7nfbzac5mhjnxpkxle 177 39 al al NNP work_infoczas7nfbzac5mhjnxpkxle 177 40 . . . work_infoczas7nfbzac5mhjnxpkxle 178 1 2007b 2007b LS work_infoczas7nfbzac5mhjnxpkxle 178 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 178 3 . . . work_infoczas7nfbzac5mhjnxpkxle 179 1 To to TO work_infoczas7nfbzac5mhjnxpkxle 179 2 create create VB work_infoczas7nfbzac5mhjnxpkxle 179 3 a a DT work_infoczas7nfbzac5mhjnxpkxle 179 4 procedure procedure NN work_infoczas7nfbzac5mhjnxpkxle 179 5 for for IN work_infoczas7nfbzac5mhjnxpkxle 179 6 production production NN work_infoczas7nfbzac5mhjnxpkxle 179 7 of of IN work_infoczas7nfbzac5mhjnxpkxle 179 8 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 179 9 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 179 10 tagged tag VBN work_infoczas7nfbzac5mhjnxpkxle 179 11 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 179 12 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 179 13 11 11 CD work_infoczas7nfbzac5mhjnxpkxle 179 14 with with IN work_infoczas7nfbzac5mhjnxpkxle 179 15 reduced reduce VBN work_infoczas7nfbzac5mhjnxpkxle 179 16 risk risk NN work_infoczas7nfbzac5mhjnxpkxle 179 17 of of IN work_infoczas7nfbzac5mhjnxpkxle 179 18 inserting insert VBG work_infoczas7nfbzac5mhjnxpkxle 179 19 the the DT work_infoczas7nfbzac5mhjnxpkxle 179 20 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 179 21 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 179 22 in in IN work_infoczas7nfbzac5mhjnxpkxle 179 23 the the DT work_infoczas7nfbzac5mhjnxpkxle 179 24 animals animal NNS work_infoczas7nfbzac5mhjnxpkxle 179 25 , , , work_infoczas7nfbzac5mhjnxpkxle 179 26 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 179 27 utilized utilize VBD work_infoczas7nfbzac5mhjnxpkxle 179 28 low low JJ work_infoczas7nfbzac5mhjnxpkxle 179 29 amounts amount NNS work_infoczas7nfbzac5mhjnxpkxle 179 30 of of IN work_infoczas7nfbzac5mhjnxpkxle 179 31 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 179 32 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 179 33 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 179 34 encoding encode VBG work_infoczas7nfbzac5mhjnxpkxle 179 35 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 179 36 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 179 37 50 50 CD work_infoczas7nfbzac5mhjnxpkxle 179 38 ng ng NN work_infoczas7nfbzac5mhjnxpkxle 179 39 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 179 40 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 179 41 during during IN work_infoczas7nfbzac5mhjnxpkxle 179 42 in in FW work_infoczas7nfbzac5mhjnxpkxle 179 43 vitro vitro FW work_infoczas7nfbzac5mhjnxpkxle 179 44 manipulation manipulation NN work_infoczas7nfbzac5mhjnxpkxle 179 45 of of IN work_infoczas7nfbzac5mhjnxpkxle 179 46 NPFs npf NNS work_infoczas7nfbzac5mhjnxpkxle 179 47 . . . work_infoczas7nfbzac5mhjnxpkxle 180 1 Evidence evidence NN work_infoczas7nfbzac5mhjnxpkxle 180 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 180 3 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 180 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 180 5 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 180 6 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 180 7 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 180 8 was be VBD work_infoczas7nfbzac5mhjnxpkxle 180 9 obtained obtain VBN work_infoczas7nfbzac5mhjnxpkxle 180 10 in in IN work_infoczas7nfbzac5mhjnxpkxle 180 11 the the DT work_infoczas7nfbzac5mhjnxpkxle 180 12 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 180 13 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 180 14 transgenic transgenic NNP work_infoczas7nfbzac5mhjnxpkxle 180 15 litter litter NN work_infoczas7nfbzac5mhjnxpkxle 180 16 . . . work_infoczas7nfbzac5mhjnxpkxle 181 1 Hence hence RB work_infoczas7nfbzac5mhjnxpkxle 181 2 , , , work_infoczas7nfbzac5mhjnxpkxle 181 3 in in IN work_infoczas7nfbzac5mhjnxpkxle 181 4 one one CD work_infoczas7nfbzac5mhjnxpkxle 181 5 of of IN work_infoczas7nfbzac5mhjnxpkxle 181 6 the the DT work_infoczas7nfbzac5mhjnxpkxle 181 7 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 181 8 , , , work_infoczas7nfbzac5mhjnxpkxle 181 9 for for IN work_infoczas7nfbzac5mhjnxpkxle 181 10 which which WDT work_infoczas7nfbzac5mhjnxpkxle 181 11 the the DT work_infoczas7nfbzac5mhjnxpkxle 181 12 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 181 13 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 181 14 were be VBD work_infoczas7nfbzac5mhjnxpkxle 181 15 carefully carefully RB work_infoczas7nfbzac5mhjnxpkxle 181 16 mapped map VBN work_infoczas7nfbzac5mhjnxpkxle 181 17 , , , work_infoczas7nfbzac5mhjnxpkxle 181 18 at at IN work_infoczas7nfbzac5mhjnxpkxle 181 19 least least JJS work_infoczas7nfbzac5mhjnxpkxle 181 20 four four CD work_infoczas7nfbzac5mhjnxpkxle 181 21 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 181 22 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 181 23 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 181 24 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 181 25 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 181 26 out out IN work_infoczas7nfbzac5mhjnxpkxle 181 27 of of IN work_infoczas7nfbzac5mhjnxpkxle 181 28 a a DT work_infoczas7nfbzac5mhjnxpkxle 181 29 total total NN work_infoczas7nfbzac5mhjnxpkxle 181 30 of of IN work_infoczas7nfbzac5mhjnxpkxle 181 31 eight eight CD work_infoczas7nfbzac5mhjnxpkxle 181 32 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 181 33 could could MD work_infoczas7nfbzac5mhjnxpkxle 181 34 be be VB work_infoczas7nfbzac5mhjnxpkxle 181 35 detected detect VBN work_infoczas7nfbzac5mhjnxpkxle 181 36 . . . work_infoczas7nfbzac5mhjnxpkxle 182 1 Integrating integrate VBG work_infoczas7nfbzac5mhjnxpkxle 182 2 viral viral JJ work_infoczas7nfbzac5mhjnxpkxle 182 3 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 182 4 are be VBP work_infoczas7nfbzac5mhjnxpkxle 182 5 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 182 6 towards towards IN work_infoczas7nfbzac5mhjnxpkxle 182 7 transcriptionally transcriptionally RB work_infoczas7nfbzac5mhjnxpkxle 182 8 active active JJ work_infoczas7nfbzac5mhjnxpkxle 182 9 regions region NNS work_infoczas7nfbzac5mhjnxpkxle 182 10 in in IN work_infoczas7nfbzac5mhjnxpkxle 182 11 the the DT work_infoczas7nfbzac5mhjnxpkxle 182 12 genome genome NN work_infoczas7nfbzac5mhjnxpkxle 182 13 due due IN work_infoczas7nfbzac5mhjnxpkxle 182 14 to to IN work_infoczas7nfbzac5mhjnxpkxle 182 15 specific specific JJ work_infoczas7nfbzac5mhjnxpkxle 182 16 interactions interaction NNS work_infoczas7nfbzac5mhjnxpkxle 182 17 between between IN work_infoczas7nfbzac5mhjnxpkxle 182 18 cellular cellular JJ work_infoczas7nfbzac5mhjnxpkxle 182 19 and and CC work_infoczas7nfbzac5mhjnxpkxle 182 20 viral viral JJ work_infoczas7nfbzac5mhjnxpkxle 182 21 proteins protein NNS work_infoczas7nfbzac5mhjnxpkxle 182 22 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 182 23 Ciuffi Ciuffi NNP work_infoczas7nfbzac5mhjnxpkxle 182 24 et et FW work_infoczas7nfbzac5mhjnxpkxle 182 25 al al NNP work_infoczas7nfbzac5mhjnxpkxle 182 26 . . . work_infoczas7nfbzac5mhjnxpkxle 183 1 2005 2005 CD work_infoczas7nfbzac5mhjnxpkxle 183 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 183 3 . . . work_infoczas7nfbzac5mhjnxpkxle 184 1 One one CD work_infoczas7nfbzac5mhjnxpkxle 184 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 184 3 the the DT work_infoczas7nfbzac5mhjnxpkxle 184 4 advantages advantage NNS work_infoczas7nfbzac5mhjnxpkxle 184 5 of of IN work_infoczas7nfbzac5mhjnxpkxle 184 6 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 184 7 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 184 8 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 184 9 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 184 10 the the DT work_infoczas7nfbzac5mhjnxpkxle 184 11 random random JJ work_infoczas7nfbzac5mhjnxpkxle 184 12 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 184 13 profile profile NN work_infoczas7nfbzac5mhjnxpkxle 184 14 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 184 15 Liu Liu NNP work_infoczas7nfbzac5mhjnxpkxle 184 16 et et NNP work_infoczas7nfbzac5mhjnxpkxle 184 17 al al NNP work_infoczas7nfbzac5mhjnxpkxle 184 18 . . . work_infoczas7nfbzac5mhjnxpkxle 185 1 2005 2005 CD work_infoczas7nfbzac5mhjnxpkxle 185 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 185 3 Staunstrup Staunstrup NNP work_infoczas7nfbzac5mhjnxpkxle 185 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 185 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 185 6 . . . work_infoczas7nfbzac5mhjnxpkxle 186 1 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 186 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 186 3 Yant Yant NNP work_infoczas7nfbzac5mhjnxpkxle 186 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 186 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 186 6 . . . work_infoczas7nfbzac5mhjnxpkxle 187 1 2005 2005 CD work_infoczas7nfbzac5mhjnxpkxle 187 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 187 3 . . . work_infoczas7nfbzac5mhjnxpkxle 188 1 Thus thus RB work_infoczas7nfbzac5mhjnxpkxle 188 2 , , , work_infoczas7nfbzac5mhjnxpkxle 188 3 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 188 4 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 188 5 are be VBP work_infoczas7nfbzac5mhjnxpkxle 188 6 less less RBR work_infoczas7nfbzac5mhjnxpkxle 188 7 likely likely JJ work_infoczas7nfbzac5mhjnxpkxle 188 8 to to TO work_infoczas7nfbzac5mhjnxpkxle 188 9 target target VB work_infoczas7nfbzac5mhjnxpkxle 188 10 genes gene NNS work_infoczas7nfbzac5mhjnxpkxle 188 11 but but CC work_infoczas7nfbzac5mhjnxpkxle 188 12 may may MD work_infoczas7nfbzac5mhjnxpkxle 188 13 be be VB work_infoczas7nfbzac5mhjnxpkxle 188 14 more more RBR work_infoczas7nfbzac5mhjnxpkxle 188 15 vulnerable vulnerable JJ work_infoczas7nfbzac5mhjnxpkxle 188 16 to to IN work_infoczas7nfbzac5mhjnxpkxle 188 17 transcriptional transcriptional JJ work_infoczas7nfbzac5mhjnxpkxle 188 18 silencing silencing NN work_infoczas7nfbzac5mhjnxpkxle 188 19 when when WRB work_infoczas7nfbzac5mhjnxpkxle 188 20 inserted insert VBN work_infoczas7nfbzac5mhjnxpkxle 188 21 in in IN work_infoczas7nfbzac5mhjnxpkxle 188 22 heterochromatin heterochromatin NNP work_infoczas7nfbzac5mhjnxpkxle 188 23 . . . work_infoczas7nfbzac5mhjnxpkxle 189 1 In in IN work_infoczas7nfbzac5mhjnxpkxle 189 2 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 189 3 lines line NNS work_infoczas7nfbzac5mhjnxpkxle 189 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 189 5 immediate immediate JJ work_infoczas7nfbzac5mhjnxpkxle 189 6 silencing silencing NN work_infoczas7nfbzac5mhjnxpkxle 189 7 of of IN work_infoczas7nfbzac5mhjnxpkxle 189 8 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 189 9 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 189 10 after after IN work_infoczas7nfbzac5mhjnxpkxle 189 11 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 189 12 can can MD work_infoczas7nfbzac5mhjnxpkxle 189 13 be be VB work_infoczas7nfbzac5mhjnxpkxle 189 14 relieved relieve VBN work_infoczas7nfbzac5mhjnxpkxle 189 15 by by IN work_infoczas7nfbzac5mhjnxpkxle 189 16 flanking flank VBG work_infoczas7nfbzac5mhjnxpkxle 189 17 the the DT work_infoczas7nfbzac5mhjnxpkxle 189 18 transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 189 19 with with IN work_infoczas7nfbzac5mhjnxpkxle 189 20 cHS4 cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 189 21 insulators insulator NNS work_infoczas7nfbzac5mhjnxpkxle 189 22 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 189 23 Dalsgaard Dalsgaard NNP work_infoczas7nfbzac5mhjnxpkxle 189 24 et et NNP work_infoczas7nfbzac5mhjnxpkxle 189 25 al al NNP work_infoczas7nfbzac5mhjnxpkxle 189 26 . . . work_infoczas7nfbzac5mhjnxpkxle 190 1 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 190 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 190 3 , , , work_infoczas7nfbzac5mhjnxpkxle 190 4 and and CC work_infoczas7nfbzac5mhjnxpkxle 190 5 downstream downstream JJ work_infoczas7nfbzac5mhjnxpkxle 190 6 analyses analysis NNS work_infoczas7nfbzac5mhjnxpkxle 190 7 of of IN work_infoczas7nfbzac5mhjnxpkxle 190 8 the the DT work_infoczas7nfbzac5mhjnxpkxle 190 9 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 190 10 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 190 11 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 190 12 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 190 13 will will MD work_infoczas7nfbzac5mhjnxpkxle 190 14 assist assist VB work_infoczas7nfbzac5mhjnxpkxle 190 15 in in IN work_infoczas7nfbzac5mhjnxpkxle 190 16 documenting document VBG work_infoczas7nfbzac5mhjnxpkxle 190 17 the the DT work_infoczas7nfbzac5mhjnxpkxle 190 18 potential potential JJ work_infoczas7nfbzac5mhjnxpkxle 190 19 benefits benefit NNS work_infoczas7nfbzac5mhjnxpkxle 190 20 of of IN work_infoczas7nfbzac5mhjnxpkxle 190 21 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 190 22 insulation insulation NN work_infoczas7nfbzac5mhjnxpkxle 190 23 . . . work_infoczas7nfbzac5mhjnxpkxle 191 1 Although although IN work_infoczas7nfbzac5mhjnxpkxle 191 2 HSB3 HSB3 NNP work_infoczas7nfbzac5mhjnxpkxle 191 3 mediated mediate VBD work_infoczas7nfbzac5mhjnxpkxle 191 4 increased increase VBD work_infoczas7nfbzac5mhjnxpkxle 191 5 stable stable JJ work_infoczas7nfbzac5mhjnxpkxle 191 6 transfection transfection NN work_infoczas7nfbzac5mhjnxpkxle 191 7 in in IN work_infoczas7nfbzac5mhjnxpkxle 191 8 NPFs NPFs NNP work_infoczas7nfbzac5mhjnxpkxle 191 9 , , , work_infoczas7nfbzac5mhjnxpkxle 191 10 at at RB work_infoczas7nfbzac5mhjnxpkxle 191 11 least least RBS work_infoczas7nfbzac5mhjnxpkxle 191 12 one one CD work_infoczas7nfbzac5mhjnxpkxle 191 13 random random JJ work_infoczas7nfbzac5mhjnxpkxle 191 14 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 191 15 containing contain VBG work_infoczas7nfbzac5mhjnxpkxle 191 16 remnants remnant NNS work_infoczas7nfbzac5mhjnxpkxle 191 17 of of IN work_infoczas7nfbzac5mhjnxpkxle 191 18 the the DT work_infoczas7nfbzac5mhjnxpkxle 191 19 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 191 20 donor donor NN work_infoczas7nfbzac5mhjnxpkxle 191 21 plasmid plasmid NNP work_infoczas7nfbzac5mhjnxpkxle 191 22 backbone backbone NN work_infoczas7nfbzac5mhjnxpkxle 191 23 was be VBD work_infoczas7nfbzac5mhjnxpkxle 191 24 identified identify VBN work_infoczas7nfbzac5mhjnxpkxle 191 25 in in IN work_infoczas7nfbzac5mhjnxpkxle 191 26 each each DT work_infoczas7nfbzac5mhjnxpkxle 191 27 of of IN work_infoczas7nfbzac5mhjnxpkxle 191 28 the the DT work_infoczas7nfbzac5mhjnxpkxle 191 29 five five CD work_infoczas7nfbzac5mhjnxpkxle 191 30 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 191 31 . . . work_infoczas7nfbzac5mhjnxpkxle 192 1 Notably notably RB work_infoczas7nfbzac5mhjnxpkxle 192 2 , , , work_infoczas7nfbzac5mhjnxpkxle 192 3 one one CD work_infoczas7nfbzac5mhjnxpkxle 192 4 of of IN work_infoczas7nfbzac5mhjnxpkxle 192 5 the the DT work_infoczas7nfbzac5mhjnxpkxle 192 6 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 192 7 was be VBD work_infoczas7nfbzac5mhjnxpkxle 192 8 found find VBN work_infoczas7nfbzac5mhjnxpkxle 192 9 to to TO work_infoczas7nfbzac5mhjnxpkxle 192 10 contain contain VB work_infoczas7nfbzac5mhjnxpkxle 192 11 two two CD work_infoczas7nfbzac5mhjnxpkxle 192 12 random random JJ work_infoczas7nfbzac5mhjnxpkxle 192 13 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 192 14 only only RB work_infoczas7nfbzac5mhjnxpkxle 192 15 . . . work_infoczas7nfbzac5mhjnxpkxle 193 1 This this DT work_infoczas7nfbzac5mhjnxpkxle 193 2 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 193 3 did do VBD work_infoczas7nfbzac5mhjnxpkxle 193 4 not not RB work_infoczas7nfbzac5mhjnxpkxle 193 5 express express VB work_infoczas7nfbzac5mhjnxpkxle 193 6 the the DT work_infoczas7nfbzac5mhjnxpkxle 193 7 transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 193 8 , , , work_infoczas7nfbzac5mhjnxpkxle 193 9 suggesting suggest VBG work_infoczas7nfbzac5mhjnxpkxle 193 10 that that IN work_infoczas7nfbzac5mhjnxpkxle 193 11 the the DT work_infoczas7nfbzac5mhjnxpkxle 193 12 two two CD work_infoczas7nfbzac5mhjnxpkxle 193 13 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 193 14 cassettes cassette NNS work_infoczas7nfbzac5mhjnxpkxle 193 15 were be VBD work_infoczas7nfbzac5mhjnxpkxle 193 16 transcriptionally transcriptionally RB work_infoczas7nfbzac5mhjnxpkxle 193 17 silenced silence VBN work_infoczas7nfbzac5mhjnxpkxle 193 18 or or CC work_infoczas7nfbzac5mhjnxpkxle 193 19 , , , work_infoczas7nfbzac5mhjnxpkxle 193 20 as as IN work_infoczas7nfbzac5mhjnxpkxle 193 21 can can MD work_infoczas7nfbzac5mhjnxpkxle 193 22 not not RB work_infoczas7nfbzac5mhjnxpkxle 193 23 be be VB work_infoczas7nfbzac5mhjnxpkxle 193 24 formally formally RB work_infoczas7nfbzac5mhjnxpkxle 193 25 excluded exclude VBN work_infoczas7nfbzac5mhjnxpkxle 193 26 , , , work_infoczas7nfbzac5mhjnxpkxle 193 27 that that IN work_infoczas7nfbzac5mhjnxpkxle 193 28 the the DT work_infoczas7nfbzac5mhjnxpkxle 193 29 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 193 30 breakage breakage NN work_infoczas7nfbzac5mhjnxpkxle 193 31 point point NN work_infoczas7nfbzac5mhjnxpkxle 193 32 in in IN work_infoczas7nfbzac5mhjnxpkxle 193 33 both both DT work_infoczas7nfbzac5mhjnxpkxle 193 34 cases case NNS work_infoczas7nfbzac5mhjnxpkxle 193 35 affected affect VBD work_infoczas7nfbzac5mhjnxpkxle 193 36 both both DT work_infoczas7nfbzac5mhjnxpkxle 193 37 genes gene NNS work_infoczas7nfbzac5mhjnxpkxle 193 38 of of IN work_infoczas7nfbzac5mhjnxpkxle 193 39 the the DT work_infoczas7nfbzac5mhjnxpkxle 193 40 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 193 41 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 193 42 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 193 43 at at IN work_infoczas7nfbzac5mhjnxpkxle 193 44 some some DT work_infoczas7nfbzac5mhjnxpkxle 193 45 point point NN work_infoczas7nfbzac5mhjnxpkxle 193 46 after after IN work_infoczas7nfbzac5mhjnxpkxle 193 47 initial initial JJ work_infoczas7nfbzac5mhjnxpkxle 193 48 selection selection NN work_infoczas7nfbzac5mhjnxpkxle 193 49 . . . work_infoczas7nfbzac5mhjnxpkxle 194 1 Transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 194 2 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 194 3 encoding encode VBG work_infoczas7nfbzac5mhjnxpkxle 194 4 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 194 5 could could MD work_infoczas7nfbzac5mhjnxpkxle 194 6 not not RB work_infoczas7nfbzac5mhjnxpkxle 194 7 be be VB work_infoczas7nfbzac5mhjnxpkxle 194 8 detected detect VBN work_infoczas7nfbzac5mhjnxpkxle 194 9 in in IN work_infoczas7nfbzac5mhjnxpkxle 194 10 any any DT work_infoczas7nfbzac5mhjnxpkxle 194 11 of of IN work_infoczas7nfbzac5mhjnxpkxle 194 12 the the DT work_infoczas7nfbzac5mhjnxpkxle 194 13 five five CD work_infoczas7nfbzac5mhjnxpkxle 194 14 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 194 15 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 194 16 . . . work_infoczas7nfbzac5mhjnxpkxle 195 1 This this DT work_infoczas7nfbzac5mhjnxpkxle 195 2 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 195 3 an an DT work_infoczas7nfbzac5mhjnxpkxle 195 4 important important JJ work_infoczas7nfbzac5mhjnxpkxle 195 5 finding finding NN work_infoczas7nfbzac5mhjnxpkxle 195 6 since since IN work_infoczas7nfbzac5mhjnxpkxle 195 7 continued continued JJ work_infoczas7nfbzac5mhjnxpkxle 195 8 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 195 9 of of IN work_infoczas7nfbzac5mhjnxpkxle 195 10 the the DT work_infoczas7nfbzac5mhjnxpkxle 195 11 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 195 12 could could MD work_infoczas7nfbzac5mhjnxpkxle 195 13 potentially potentially RB work_infoczas7nfbzac5mhjnxpkxle 195 14 facilitate facilitate VB work_infoczas7nfbzac5mhjnxpkxle 195 15 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 195 16 re re NN work_infoczas7nfbzac5mhjnxpkxle 195 17 - - NN work_infoczas7nfbzac5mhjnxpkxle 195 18 mobilization mobilization NN work_infoczas7nfbzac5mhjnxpkxle 195 19 and and CC work_infoczas7nfbzac5mhjnxpkxle 195 20 re re NN work_infoczas7nfbzac5mhjnxpkxle 195 21 - - NN work_infoczas7nfbzac5mhjnxpkxle 195 22 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 195 23 , , , work_infoczas7nfbzac5mhjnxpkxle 195 24 resulting result VBG work_infoczas7nfbzac5mhjnxpkxle 195 25 in in IN work_infoczas7nfbzac5mhjnxpkxle 195 26 reduced reduce VBN work_infoczas7nfbzac5mhjnxpkxle 195 27 overall overall JJ work_infoczas7nfbzac5mhjnxpkxle 195 28 stability stability NN work_infoczas7nfbzac5mhjnxpkxle 195 29 of of IN work_infoczas7nfbzac5mhjnxpkxle 195 30 the the DT work_infoczas7nfbzac5mhjnxpkxle 195 31 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 195 32 model model NN work_infoczas7nfbzac5mhjnxpkxle 195 33 . . . work_infoczas7nfbzac5mhjnxpkxle 196 1 Although although IN work_infoczas7nfbzac5mhjnxpkxle 196 2 this this DT work_infoczas7nfbzac5mhjnxpkxle 196 3 aspect aspect NN work_infoczas7nfbzac5mhjnxpkxle 196 4 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 196 5 rarely rarely RB work_infoczas7nfbzac5mhjnxpkxle 196 6 experimentally experimentally RB work_infoczas7nfbzac5mhjnxpkxle 196 7 addressed address VBN work_infoczas7nfbzac5mhjnxpkxle 196 8 in in IN work_infoczas7nfbzac5mhjnxpkxle 196 9 reports report NNS work_infoczas7nfbzac5mhjnxpkxle 196 10 describing describe VBG work_infoczas7nfbzac5mhjnxpkxle 196 11 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 196 12 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 196 13 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 196 14 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 196 15 delivery delivery NN work_infoczas7nfbzac5mhjnxpkxle 196 16 , , , work_infoczas7nfbzac5mhjnxpkxle 196 17 methods method NNS work_infoczas7nfbzac5mhjnxpkxle 196 18 for for IN work_infoczas7nfbzac5mhjnxpkxle 196 19 co co NN work_infoczas7nfbzac5mhjnxpkxle 196 20 - - NN work_infoczas7nfbzac5mhjnxpkxle 196 21 delivery delivery NN work_infoczas7nfbzac5mhjnxpkxle 196 22 of of IN work_infoczas7nfbzac5mhjnxpkxle 196 23 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 196 24 donor donor NN work_infoczas7nfbzac5mhjnxpkxle 196 25 plasmid plasmid NNP work_infoczas7nfbzac5mhjnxpkxle 196 26 with with IN work_infoczas7nfbzac5mhjnxpkxle 196 27 in in FW work_infoczas7nfbzac5mhjnxpkxle 196 28 vitro vitro FW work_infoczas7nfbzac5mhjnxpkxle 196 29 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 196 30 synthesized synthesize VBN work_infoczas7nfbzac5mhjnxpkxle 196 31 mRNA mrna NN work_infoczas7nfbzac5mhjnxpkxle 196 32 encoding encode VBG work_infoczas7nfbzac5mhjnxpkxle 196 33 the the DT work_infoczas7nfbzac5mhjnxpkxle 196 34 12 12 CD work_infoczas7nfbzac5mhjnxpkxle 196 35 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 196 36 to to IN work_infoczas7nfbzac5mhjnxpkxle 196 37 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 196 38 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 196 39 have have VBP work_infoczas7nfbzac5mhjnxpkxle 196 40 been be VBN work_infoczas7nfbzac5mhjnxpkxle 196 41 described describe VBN work_infoczas7nfbzac5mhjnxpkxle 196 42 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 196 43 Wilber Wilber NNP work_infoczas7nfbzac5mhjnxpkxle 196 44 et et FW work_infoczas7nfbzac5mhjnxpkxle 196 45 al al NNP work_infoczas7nfbzac5mhjnxpkxle 196 46 . . . work_infoczas7nfbzac5mhjnxpkxle 197 1 2006 2006 CD work_infoczas7nfbzac5mhjnxpkxle 197 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 197 3 and and CC work_infoczas7nfbzac5mhjnxpkxle 197 4 may may MD work_infoczas7nfbzac5mhjnxpkxle 197 5 be be VB work_infoczas7nfbzac5mhjnxpkxle 197 6 applicable applicable JJ work_infoczas7nfbzac5mhjnxpkxle 197 7 in in IN work_infoczas7nfbzac5mhjnxpkxle 197 8 protocols protocol NNS work_infoczas7nfbzac5mhjnxpkxle 197 9 of of IN work_infoczas7nfbzac5mhjnxpkxle 197 10 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 197 11 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 197 12 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 197 13 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 197 14 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 197 15 . . . work_infoczas7nfbzac5mhjnxpkxle 198 1 Also also RB work_infoczas7nfbzac5mhjnxpkxle 198 2 , , , work_infoczas7nfbzac5mhjnxpkxle 198 3 the the DT work_infoczas7nfbzac5mhjnxpkxle 198 4 use use NN work_infoczas7nfbzac5mhjnxpkxle 198 5 of of IN work_infoczas7nfbzac5mhjnxpkxle 198 6 in in FW work_infoczas7nfbzac5mhjnxpkxle 198 7 vitro vitro FW work_infoczas7nfbzac5mhjnxpkxle 198 8 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 198 9 transcribed transcribe VBN work_infoczas7nfbzac5mhjnxpkxle 198 10 mRNA mRNA NNP work_infoczas7nfbzac5mhjnxpkxle 198 11 as as IN work_infoczas7nfbzac5mhjnxpkxle 198 12 a a DT work_infoczas7nfbzac5mhjnxpkxle 198 13 source source NN work_infoczas7nfbzac5mhjnxpkxle 198 14 of of IN work_infoczas7nfbzac5mhjnxpkxle 198 15 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 198 16 has have VBZ work_infoczas7nfbzac5mhjnxpkxle 198 17 been be VBN work_infoczas7nfbzac5mhjnxpkxle 198 18 successful successful JJ work_infoczas7nfbzac5mhjnxpkxle 198 19 in in IN work_infoczas7nfbzac5mhjnxpkxle 198 20 early early JJ work_infoczas7nfbzac5mhjnxpkxle 198 21 embryos embryo NNS work_infoczas7nfbzac5mhjnxpkxle 198 22 of of IN work_infoczas7nfbzac5mhjnxpkxle 198 23 frog frog NN work_infoczas7nfbzac5mhjnxpkxle 198 24 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 198 25 Sinzelle Sinzelle NNP work_infoczas7nfbzac5mhjnxpkxle 198 26 et et FW work_infoczas7nfbzac5mhjnxpkxle 198 27 al al NNP work_infoczas7nfbzac5mhjnxpkxle 198 28 . . . work_infoczas7nfbzac5mhjnxpkxle 199 1 2006 2006 CD work_infoczas7nfbzac5mhjnxpkxle 199 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 199 3 , , , work_infoczas7nfbzac5mhjnxpkxle 199 4 zebrafish zebrafish NNP work_infoczas7nfbzac5mhjnxpkxle 199 5 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 199 6 Davidson Davidson NNP work_infoczas7nfbzac5mhjnxpkxle 199 7 et et NNP work_infoczas7nfbzac5mhjnxpkxle 199 8 al al NNP work_infoczas7nfbzac5mhjnxpkxle 199 9 . . . work_infoczas7nfbzac5mhjnxpkxle 200 1 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 200 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 200 3 , , , work_infoczas7nfbzac5mhjnxpkxle 200 4 and and CC work_infoczas7nfbzac5mhjnxpkxle 200 5 mouse mouse NN work_infoczas7nfbzac5mhjnxpkxle 200 6 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 200 7 Dupuy Dupuy NNP work_infoczas7nfbzac5mhjnxpkxle 200 8 et et NNP work_infoczas7nfbzac5mhjnxpkxle 200 9 al al NNP work_infoczas7nfbzac5mhjnxpkxle 200 10 . . . work_infoczas7nfbzac5mhjnxpkxle 201 1 2002 2002 CD work_infoczas7nfbzac5mhjnxpkxle 201 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 201 3 . . . work_infoczas7nfbzac5mhjnxpkxle 202 1 Cloning clone VBG work_infoczas7nfbzac5mhjnxpkxle 202 2 based base VBN work_infoczas7nfbzac5mhjnxpkxle 202 3 on on IN work_infoczas7nfbzac5mhjnxpkxle 202 4 a a DT work_infoczas7nfbzac5mhjnxpkxle 202 5 pool pool NN work_infoczas7nfbzac5mhjnxpkxle 202 6 consisting consisting NN work_infoczas7nfbzac5mhjnxpkxle 202 7 of of IN work_infoczas7nfbzac5mhjnxpkxle 202 8 80 80 CD work_infoczas7nfbzac5mhjnxpkxle 202 9 NPF NPF NNP work_infoczas7nfbzac5mhjnxpkxle 202 10 clones clone NNS work_infoczas7nfbzac5mhjnxpkxle 202 11 resulted result VBD work_infoczas7nfbzac5mhjnxpkxle 202 12 in in IN work_infoczas7nfbzac5mhjnxpkxle 202 13 birth birth NN work_infoczas7nfbzac5mhjnxpkxle 202 14 of of IN work_infoczas7nfbzac5mhjnxpkxle 202 15 one one CD work_infoczas7nfbzac5mhjnxpkxle 202 16 set set NN work_infoczas7nfbzac5mhjnxpkxle 202 17 of of IN work_infoczas7nfbzac5mhjnxpkxle 202 18 genetically genetically RB work_infoczas7nfbzac5mhjnxpkxle 202 19 identical identical JJ work_infoczas7nfbzac5mhjnxpkxle 202 20 twins twin NNS work_infoczas7nfbzac5mhjnxpkxle 202 21 and and CC work_infoczas7nfbzac5mhjnxpkxle 202 22 a a DT work_infoczas7nfbzac5mhjnxpkxle 202 23 pair pair NN work_infoczas7nfbzac5mhjnxpkxle 202 24 of of IN work_infoczas7nfbzac5mhjnxpkxle 202 25 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 202 26 originating originate VBG work_infoczas7nfbzac5mhjnxpkxle 202 27 from from IN work_infoczas7nfbzac5mhjnxpkxle 202 28 a a DT work_infoczas7nfbzac5mhjnxpkxle 202 29 single single JJ work_infoczas7nfbzac5mhjnxpkxle 202 30 NPF NPF NNP work_infoczas7nfbzac5mhjnxpkxle 202 31 clone clone NN work_infoczas7nfbzac5mhjnxpkxle 202 32 . . . work_infoczas7nfbzac5mhjnxpkxle 203 1 This this DT work_infoczas7nfbzac5mhjnxpkxle 203 2 indicates indicate VBZ work_infoczas7nfbzac5mhjnxpkxle 203 3 that that IN work_infoczas7nfbzac5mhjnxpkxle 203 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 203 5 genetic genetic JJ work_infoczas7nfbzac5mhjnxpkxle 203 6 engineering engineering NN work_infoczas7nfbzac5mhjnxpkxle 203 7 process process NN work_infoczas7nfbzac5mhjnxpkxle 203 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 203 9 cloning clone VBG work_infoczas7nfbzac5mhjnxpkxle 203 10 by by IN work_infoczas7nfbzac5mhjnxpkxle 203 11 HMC HMC NNP work_infoczas7nfbzac5mhjnxpkxle 203 12 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 203 13 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 203 14 SCNT SCNT NNP work_infoczas7nfbzac5mhjnxpkxle 203 15 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 203 16 accompanied accompany VBN work_infoczas7nfbzac5mhjnxpkxle 203 17 by by IN work_infoczas7nfbzac5mhjnxpkxle 203 18 a a DT work_infoczas7nfbzac5mhjnxpkxle 203 19 selection selection NN work_infoczas7nfbzac5mhjnxpkxle 203 20 pressure pressure NN work_infoczas7nfbzac5mhjnxpkxle 203 21 . . . work_infoczas7nfbzac5mhjnxpkxle 204 1 Whether whether IN work_infoczas7nfbzac5mhjnxpkxle 204 2 this this DT work_infoczas7nfbzac5mhjnxpkxle 204 3 pressure pressure NN work_infoczas7nfbzac5mhjnxpkxle 204 4 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 204 5 in in IN work_infoczas7nfbzac5mhjnxpkxle 204 6 effect effect NN work_infoczas7nfbzac5mhjnxpkxle 204 7 during during IN work_infoczas7nfbzac5mhjnxpkxle 204 8 in in FW work_infoczas7nfbzac5mhjnxpkxle 204 9 vitro vitro FW work_infoczas7nfbzac5mhjnxpkxle 204 10 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 204 11 expansion expansion NN work_infoczas7nfbzac5mhjnxpkxle 204 12 or or CC work_infoczas7nfbzac5mhjnxpkxle 204 13 during during IN work_infoczas7nfbzac5mhjnxpkxle 204 14 blastocyst blastocyst NN work_infoczas7nfbzac5mhjnxpkxle 204 15 or or CC work_infoczas7nfbzac5mhjnxpkxle 204 16 embryo embryo NN work_infoczas7nfbzac5mhjnxpkxle 204 17 development development NN work_infoczas7nfbzac5mhjnxpkxle 204 18 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 204 19 not not RB work_infoczas7nfbzac5mhjnxpkxle 204 20 clear clear JJ work_infoczas7nfbzac5mhjnxpkxle 204 21 . . . work_infoczas7nfbzac5mhjnxpkxle 205 1 Also also RB work_infoczas7nfbzac5mhjnxpkxle 205 2 , , , work_infoczas7nfbzac5mhjnxpkxle 205 3 it -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 205 4 remains remain VBZ work_infoczas7nfbzac5mhjnxpkxle 205 5 a a DT work_infoczas7nfbzac5mhjnxpkxle 205 6 matter matter NN work_infoczas7nfbzac5mhjnxpkxle 205 7 of of IN work_infoczas7nfbzac5mhjnxpkxle 205 8 speculation speculation NN work_infoczas7nfbzac5mhjnxpkxle 205 9 whether whether IN work_infoczas7nfbzac5mhjnxpkxle 205 10 these these DT work_infoczas7nfbzac5mhjnxpkxle 205 11 observations observation NNS work_infoczas7nfbzac5mhjnxpkxle 205 12 reflect reflect VBP work_infoczas7nfbzac5mhjnxpkxle 205 13 any any DT work_infoczas7nfbzac5mhjnxpkxle 205 14 toxicity toxicity NN work_infoczas7nfbzac5mhjnxpkxle 205 15 related relate VBN work_infoczas7nfbzac5mhjnxpkxle 205 16 to to IN work_infoczas7nfbzac5mhjnxpkxle 205 17 the the DT work_infoczas7nfbzac5mhjnxpkxle 205 18 actions action NNS work_infoczas7nfbzac5mhjnxpkxle 205 19 of of IN work_infoczas7nfbzac5mhjnxpkxle 205 20 the the DT work_infoczas7nfbzac5mhjnxpkxle 205 21 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 205 22 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 205 23 . . . work_infoczas7nfbzac5mhjnxpkxle 206 1 Despite despite IN work_infoczas7nfbzac5mhjnxpkxle 206 2 the the DT work_infoczas7nfbzac5mhjnxpkxle 206 3 fact fact NN work_infoczas7nfbzac5mhjnxpkxle 206 4 that that IN work_infoczas7nfbzac5mhjnxpkxle 206 5 four four CD work_infoczas7nfbzac5mhjnxpkxle 206 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 206 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 206 8 five five CD work_infoczas7nfbzac5mhjnxpkxle 206 9 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 206 10 contained contain VBN work_infoczas7nfbzac5mhjnxpkxle 206 11 at at IN work_infoczas7nfbzac5mhjnxpkxle 206 12 least least JJS work_infoczas7nfbzac5mhjnxpkxle 206 13 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 206 14 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 206 15 , , , work_infoczas7nfbzac5mhjnxpkxle 206 16 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 206 17 find find VBP work_infoczas7nfbzac5mhjnxpkxle 206 18 it -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 206 19 unlikely unlikely JJ work_infoczas7nfbzac5mhjnxpkxle 206 20 that that IN work_infoczas7nfbzac5mhjnxpkxle 206 21 these these DT work_infoczas7nfbzac5mhjnxpkxle 206 22 clones clone NNS work_infoczas7nfbzac5mhjnxpkxle 206 23 had have VBD work_infoczas7nfbzac5mhjnxpkxle 206 24 a a DT work_infoczas7nfbzac5mhjnxpkxle 206 25 selective selective JJ work_infoczas7nfbzac5mhjnxpkxle 206 26 advantage advantage NN work_infoczas7nfbzac5mhjnxpkxle 206 27 due due IN work_infoczas7nfbzac5mhjnxpkxle 206 28 to to IN work_infoczas7nfbzac5mhjnxpkxle 206 29 their -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 206 30 high high JJ work_infoczas7nfbzac5mhjnxpkxle 206 31 copy copy NN work_infoczas7nfbzac5mhjnxpkxle 206 32 numbers number NNS work_infoczas7nfbzac5mhjnxpkxle 206 33 . . . work_infoczas7nfbzac5mhjnxpkxle 207 1 However however RB work_infoczas7nfbzac5mhjnxpkxle 207 2 , , , work_infoczas7nfbzac5mhjnxpkxle 207 3 our -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 207 4 data datum NNS work_infoczas7nfbzac5mhjnxpkxle 207 5 suggest suggest VBP work_infoczas7nfbzac5mhjnxpkxle 207 6 that that IN work_infoczas7nfbzac5mhjnxpkxle 207 7 a a DT work_infoczas7nfbzac5mhjnxpkxle 207 8 cloning clone VBG work_infoczas7nfbzac5mhjnxpkxle 207 9 strategy strategy NN work_infoczas7nfbzac5mhjnxpkxle 207 10 based base VBN work_infoczas7nfbzac5mhjnxpkxle 207 11 on on IN work_infoczas7nfbzac5mhjnxpkxle 207 12 expanded expand VBN work_infoczas7nfbzac5mhjnxpkxle 207 13 pools pool NNS work_infoczas7nfbzac5mhjnxpkxle 207 14 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 207 15 more more RBR work_infoczas7nfbzac5mhjnxpkxle 207 16 likely likely JJ work_infoczas7nfbzac5mhjnxpkxle 207 17 to to TO work_infoczas7nfbzac5mhjnxpkxle 207 18 succeed succeed VB work_infoczas7nfbzac5mhjnxpkxle 207 19 , , , work_infoczas7nfbzac5mhjnxpkxle 207 20 less less JJR work_infoczas7nfbzac5mhjnxpkxle 207 21 labor labor NN work_infoczas7nfbzac5mhjnxpkxle 207 22 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 207 23 intensive intensive JJ work_infoczas7nfbzac5mhjnxpkxle 207 24 , , , work_infoczas7nfbzac5mhjnxpkxle 207 25 and and CC work_infoczas7nfbzac5mhjnxpkxle 207 26 cheaper cheap JJR work_infoczas7nfbzac5mhjnxpkxle 207 27 than than IN work_infoczas7nfbzac5mhjnxpkxle 207 28 cloning clone VBG work_infoczas7nfbzac5mhjnxpkxle 207 29 using use VBG work_infoczas7nfbzac5mhjnxpkxle 207 30 individual individual JJ work_infoczas7nfbzac5mhjnxpkxle 207 31 clones clone NNS work_infoczas7nfbzac5mhjnxpkxle 207 32 . . . work_infoczas7nfbzac5mhjnxpkxle 208 1 Moreover moreover RB work_infoczas7nfbzac5mhjnxpkxle 208 2 , , , work_infoczas7nfbzac5mhjnxpkxle 208 3 by by IN work_infoczas7nfbzac5mhjnxpkxle 208 4 growing grow VBG work_infoczas7nfbzac5mhjnxpkxle 208 5 pools pool NNS work_infoczas7nfbzac5mhjnxpkxle 208 6 rather rather RB work_infoczas7nfbzac5mhjnxpkxle 208 7 than than IN work_infoczas7nfbzac5mhjnxpkxle 208 8 individual individual JJ work_infoczas7nfbzac5mhjnxpkxle 208 9 drug drug NN work_infoczas7nfbzac5mhjnxpkxle 208 10 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 208 11 resistant resistant JJ work_infoczas7nfbzac5mhjnxpkxle 208 12 clones clone NNS work_infoczas7nfbzac5mhjnxpkxle 208 13 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 208 14 could could MD work_infoczas7nfbzac5mhjnxpkxle 208 15 minimize minimize VB work_infoczas7nfbzac5mhjnxpkxle 208 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 208 17 number number NN work_infoczas7nfbzac5mhjnxpkxle 208 18 of of IN work_infoczas7nfbzac5mhjnxpkxle 208 19 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 208 20 cycles cycle NNS work_infoczas7nfbzac5mhjnxpkxle 208 21 and and CC work_infoczas7nfbzac5mhjnxpkxle 208 22 limit limit VB work_infoczas7nfbzac5mhjnxpkxle 208 23 the the DT work_infoczas7nfbzac5mhjnxpkxle 208 24 risk risk NN work_infoczas7nfbzac5mhjnxpkxle 208 25 of of IN work_infoczas7nfbzac5mhjnxpkxle 208 26 chromosomal chromosomal JJ work_infoczas7nfbzac5mhjnxpkxle 208 27 aberrations aberration NNS work_infoczas7nfbzac5mhjnxpkxle 208 28 during during IN work_infoczas7nfbzac5mhjnxpkxle 208 29 in in FW work_infoczas7nfbzac5mhjnxpkxle 208 30 vitro vitro FW work_infoczas7nfbzac5mhjnxpkxle 208 31 culturing culturing NN work_infoczas7nfbzac5mhjnxpkxle 208 32 of of IN work_infoczas7nfbzac5mhjnxpkxle 208 33 the the DT work_infoczas7nfbzac5mhjnxpkxle 208 34 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 208 35 . . . work_infoczas7nfbzac5mhjnxpkxle 209 1 Retro- Retro- NNP work_infoczas7nfbzac5mhjnxpkxle 209 2 and and CC work_infoczas7nfbzac5mhjnxpkxle 209 3 lentiviral lentiviral JJ work_infoczas7nfbzac5mhjnxpkxle 209 4 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 209 5 are be VBP work_infoczas7nfbzac5mhjnxpkxle 209 6 established establish VBN work_infoczas7nfbzac5mhjnxpkxle 209 7 potent potent JJ work_infoczas7nfbzac5mhjnxpkxle 209 8 tools tool NNS work_infoczas7nfbzac5mhjnxpkxle 209 9 for for IN work_infoczas7nfbzac5mhjnxpkxle 209 10 stable stable JJ work_infoczas7nfbzac5mhjnxpkxle 209 11 modification modification NN work_infoczas7nfbzac5mhjnxpkxle 209 12 of of IN work_infoczas7nfbzac5mhjnxpkxle 209 13 porcine porcine JJ work_infoczas7nfbzac5mhjnxpkxle 209 14 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 209 15 donor donor NN work_infoczas7nfbzac5mhjnxpkxle 209 16 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 209 17 for for IN work_infoczas7nfbzac5mhjnxpkxle 209 18 SCNT SCNT NNP work_infoczas7nfbzac5mhjnxpkxle 209 19 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 209 20 Lai Lai NNP work_infoczas7nfbzac5mhjnxpkxle 209 21 et et NNP work_infoczas7nfbzac5mhjnxpkxle 209 22 al al NNP work_infoczas7nfbzac5mhjnxpkxle 209 23 . . . work_infoczas7nfbzac5mhjnxpkxle 210 1 2002 2002 CD work_infoczas7nfbzac5mhjnxpkxle 210 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 210 3 Park Park NNP work_infoczas7nfbzac5mhjnxpkxle 210 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 210 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 210 6 . . . work_infoczas7nfbzac5mhjnxpkxle 211 1 2001 2001 CD work_infoczas7nfbzac5mhjnxpkxle 211 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 211 3 Park Park NNP work_infoczas7nfbzac5mhjnxpkxle 211 4 et et FW work_infoczas7nfbzac5mhjnxpkxle 211 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 211 6 . . . work_infoczas7nfbzac5mhjnxpkxle 212 1 2002 2002 CD work_infoczas7nfbzac5mhjnxpkxle 212 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 212 3 and and CC work_infoczas7nfbzac5mhjnxpkxle 212 4 have have VBP work_infoczas7nfbzac5mhjnxpkxle 212 5 been be VBN work_infoczas7nfbzac5mhjnxpkxle 212 6 successfully successfully RB work_infoczas7nfbzac5mhjnxpkxle 212 7 used use VBN work_infoczas7nfbzac5mhjnxpkxle 212 8 for for IN work_infoczas7nfbzac5mhjnxpkxle 212 9 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 212 10 delivery delivery NN work_infoczas7nfbzac5mhjnxpkxle 212 11 to to IN work_infoczas7nfbzac5mhjnxpkxle 212 12 porcine porcine VB work_infoczas7nfbzac5mhjnxpkxle 212 13 embryos embryo NNS work_infoczas7nfbzac5mhjnxpkxle 212 14 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 212 15 Cabot Cabot NNP work_infoczas7nfbzac5mhjnxpkxle 212 16 et et NNP work_infoczas7nfbzac5mhjnxpkxle 212 17 al al NNP work_infoczas7nfbzac5mhjnxpkxle 212 18 . . . work_infoczas7nfbzac5mhjnxpkxle 213 1 2001 2001 CD work_infoczas7nfbzac5mhjnxpkxle 213 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 213 3 Hofmann Hofmann NNP work_infoczas7nfbzac5mhjnxpkxle 213 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 213 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 213 6 . . . work_infoczas7nfbzac5mhjnxpkxle 214 1 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 214 2 ; ; : work_infoczas7nfbzac5mhjnxpkxle 214 3 Whitelaw Whitelaw NNP work_infoczas7nfbzac5mhjnxpkxle 214 4 et et NNP work_infoczas7nfbzac5mhjnxpkxle 214 5 al al NNP work_infoczas7nfbzac5mhjnxpkxle 214 6 . . . work_infoczas7nfbzac5mhjnxpkxle 215 1 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 215 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 215 3 . . . work_infoczas7nfbzac5mhjnxpkxle 216 1 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 216 2 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 216 3 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 216 4 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 216 5 combined combine VBN work_infoczas7nfbzac5mhjnxpkxle 216 6 with with IN work_infoczas7nfbzac5mhjnxpkxle 216 7 SCNT SCNT NNP work_infoczas7nfbzac5mhjnxpkxle 216 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 216 9 HMC HMC NNP work_infoczas7nfbzac5mhjnxpkxle 216 10 represents represent VBZ work_infoczas7nfbzac5mhjnxpkxle 216 11 a a DT work_infoczas7nfbzac5mhjnxpkxle 216 12 nonviral nonviral JJ work_infoczas7nfbzac5mhjnxpkxle 216 13 alternative alternative NN work_infoczas7nfbzac5mhjnxpkxle 216 14 that that WDT work_infoczas7nfbzac5mhjnxpkxle 216 15 offers offer VBZ work_infoczas7nfbzac5mhjnxpkxle 216 16 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 216 17 of of IN work_infoczas7nfbzac5mhjnxpkxle 216 18 a a DT work_infoczas7nfbzac5mhjnxpkxle 216 19 defined define VBN work_infoczas7nfbzac5mhjnxpkxle 216 20 genetic genetic JJ work_infoczas7nfbzac5mhjnxpkxle 216 21 unit unit NN work_infoczas7nfbzac5mhjnxpkxle 216 22 , , , work_infoczas7nfbzac5mhjnxpkxle 216 23 strong strong JJ work_infoczas7nfbzac5mhjnxpkxle 216 24 systemic systemic JJ work_infoczas7nfbzac5mhjnxpkxle 216 25 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 216 26 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 216 27 , , , work_infoczas7nfbzac5mhjnxpkxle 216 28 and and CC work_infoczas7nfbzac5mhjnxpkxle 216 29 possibilities possibility NNS work_infoczas7nfbzac5mhjnxpkxle 216 30 of of IN work_infoczas7nfbzac5mhjnxpkxle 216 31 multicopy multicopy JJ work_infoczas7nfbzac5mhjnxpkxle 216 32 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 216 33 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 216 34 . . . work_infoczas7nfbzac5mhjnxpkxle 217 1 Still still RB work_infoczas7nfbzac5mhjnxpkxle 217 2 , , , work_infoczas7nfbzac5mhjnxpkxle 217 3 the the DT work_infoczas7nfbzac5mhjnxpkxle 217 4 moderate moderate JJ work_infoczas7nfbzac5mhjnxpkxle 217 5 efficiency efficiency NN work_infoczas7nfbzac5mhjnxpkxle 217 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 217 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 217 8 cloning clone VBG work_infoczas7nfbzac5mhjnxpkxle 217 9 procedure procedure NN work_infoczas7nfbzac5mhjnxpkxle 217 10 requires require VBZ work_infoczas7nfbzac5mhjnxpkxle 217 11 that that IN work_infoczas7nfbzac5mhjnxpkxle 217 12 a a DT work_infoczas7nfbzac5mhjnxpkxle 217 13 significant significant JJ work_infoczas7nfbzac5mhjnxpkxle 217 14 amount amount NN work_infoczas7nfbzac5mhjnxpkxle 217 15 of of IN work_infoczas7nfbzac5mhjnxpkxle 217 16 nuclear nuclear JJ work_infoczas7nfbzac5mhjnxpkxle 217 17 transfers transfer NNS work_infoczas7nfbzac5mhjnxpkxle 217 18 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 217 19 performed perform VBN work_infoczas7nfbzac5mhjnxpkxle 217 20 . . . work_infoczas7nfbzac5mhjnxpkxle 218 1 In in IN work_infoczas7nfbzac5mhjnxpkxle 218 2 our -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 218 3 hands hand NNS work_infoczas7nfbzac5mhjnxpkxle 218 4 , , , work_infoczas7nfbzac5mhjnxpkxle 218 5 102 102 CD work_infoczas7nfbzac5mhjnxpkxle 218 6 blastocysts blastocyst NNS work_infoczas7nfbzac5mhjnxpkxle 218 7 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 218 8 resulting result VBG work_infoczas7nfbzac5mhjnxpkxle 218 9 from from IN work_infoczas7nfbzac5mhjnxpkxle 218 10 more more JJR work_infoczas7nfbzac5mhjnxpkxle 218 11 than than IN work_infoczas7nfbzac5mhjnxpkxle 218 12 250 250 CD work_infoczas7nfbzac5mhjnxpkxle 218 13 reconstructed reconstructed JJ work_infoczas7nfbzac5mhjnxpkxle 218 14 embryos embryo NNS work_infoczas7nfbzac5mhjnxpkxle 218 15 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 218 16 were be VBD work_infoczas7nfbzac5mhjnxpkxle 218 17 transferred transfer VBN work_infoczas7nfbzac5mhjnxpkxle 218 18 to to IN work_infoczas7nfbzac5mhjnxpkxle 218 19 the the DT work_infoczas7nfbzac5mhjnxpkxle 218 20 surrogate surrogate JJ work_infoczas7nfbzac5mhjnxpkxle 218 21 sow sow NN work_infoczas7nfbzac5mhjnxpkxle 218 22 to to TO work_infoczas7nfbzac5mhjnxpkxle 218 23 create create VB work_infoczas7nfbzac5mhjnxpkxle 218 24 a a DT work_infoczas7nfbzac5mhjnxpkxle 218 25 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 218 26 litter litter NN work_infoczas7nfbzac5mhjnxpkxle 218 27 of of IN work_infoczas7nfbzac5mhjnxpkxle 218 28 eight eight CD work_infoczas7nfbzac5mhjnxpkxle 218 29 piglets piglet NNS work_infoczas7nfbzac5mhjnxpkxle 218 30 . . . work_infoczas7nfbzac5mhjnxpkxle 219 1 Five five CD work_infoczas7nfbzac5mhjnxpkxle 219 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 219 3 these these DT work_infoczas7nfbzac5mhjnxpkxle 219 4 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 219 5 survived survive VBD work_infoczas7nfbzac5mhjnxpkxle 219 6 past past RB work_infoczas7nfbzac5mhjnxpkxle 219 7 weaning weaning NN work_infoczas7nfbzac5mhjnxpkxle 219 8 , , , work_infoczas7nfbzac5mhjnxpkxle 219 9 whereas whereas IN work_infoczas7nfbzac5mhjnxpkxle 219 10 three three CD work_infoczas7nfbzac5mhjnxpkxle 219 11 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 219 12 died die VBD work_infoczas7nfbzac5mhjnxpkxle 219 13 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 219 14 without without IN work_infoczas7nfbzac5mhjnxpkxle 219 15 signs sign NNS work_infoczas7nfbzac5mhjnxpkxle 219 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 219 17 any any DT work_infoczas7nfbzac5mhjnxpkxle 219 18 macroscopic macroscopic JJ work_infoczas7nfbzac5mhjnxpkxle 219 19 malformations malformation NNS work_infoczas7nfbzac5mhjnxpkxle 219 20 , , , work_infoczas7nfbzac5mhjnxpkxle 219 21 as as IN work_infoczas7nfbzac5mhjnxpkxle 219 22 suggested suggest VBN work_infoczas7nfbzac5mhjnxpkxle 219 23 by by IN work_infoczas7nfbzac5mhjnxpkxle 219 24 the the DT work_infoczas7nfbzac5mhjnxpkxle 219 25 post post JJ work_infoczas7nfbzac5mhjnxpkxle 219 26 - - JJ work_infoczas7nfbzac5mhjnxpkxle 219 27 mortem mortem JJ work_infoczas7nfbzac5mhjnxpkxle 219 28 examination examination NN work_infoczas7nfbzac5mhjnxpkxle 219 29 . . . work_infoczas7nfbzac5mhjnxpkxle 220 1 Reports report NNS work_infoczas7nfbzac5mhjnxpkxle 220 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 220 3 lentiviral lentiviral JJ work_infoczas7nfbzac5mhjnxpkxle 220 4 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 220 5 based base VBN work_infoczas7nfbzac5mhjnxpkxle 220 6 on on IN work_infoczas7nfbzac5mhjnxpkxle 220 7 injection injection NN work_infoczas7nfbzac5mhjnxpkxle 220 8 of of IN work_infoczas7nfbzac5mhjnxpkxle 220 9 viral viral JJ work_infoczas7nfbzac5mhjnxpkxle 220 10 particles particle NNS work_infoczas7nfbzac5mhjnxpkxle 220 11 into into IN work_infoczas7nfbzac5mhjnxpkxle 220 12 the the DT work_infoczas7nfbzac5mhjnxpkxle 220 13 perivitelline perivitelline NN work_infoczas7nfbzac5mhjnxpkxle 220 14 space space NN work_infoczas7nfbzac5mhjnxpkxle 220 15 of of IN work_infoczas7nfbzac5mhjnxpkxle 220 16 single single JJ work_infoczas7nfbzac5mhjnxpkxle 220 17 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 220 18 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 220 19 embryos embryo NNS work_infoczas7nfbzac5mhjnxpkxle 220 20 have have VBP work_infoczas7nfbzac5mhjnxpkxle 220 21 shown show VBN work_infoczas7nfbzac5mhjnxpkxle 220 22 birth birth NN work_infoczas7nfbzac5mhjnxpkxle 220 23 of of IN work_infoczas7nfbzac5mhjnxpkxle 220 24 as as RB work_infoczas7nfbzac5mhjnxpkxle 220 25 many many JJ work_infoczas7nfbzac5mhjnxpkxle 220 26 as as IN work_infoczas7nfbzac5mhjnxpkxle 220 27 34 34 CD work_infoczas7nfbzac5mhjnxpkxle 220 28 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 220 29 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 220 30 from from IN work_infoczas7nfbzac5mhjnxpkxle 220 31 244 244 CD work_infoczas7nfbzac5mhjnxpkxle 220 32 zygotes zygote NNS work_infoczas7nfbzac5mhjnxpkxle 220 33 treated treat VBN work_infoczas7nfbzac5mhjnxpkxle 220 34 with with IN work_infoczas7nfbzac5mhjnxpkxle 220 35 human human JJ work_infoczas7nfbzac5mhjnxpkxle 220 36 immunodeficiency immunodeficiency NN work_infoczas7nfbzac5mhjnxpkxle 220 37 virus virus NN work_infoczas7nfbzac5mhjnxpkxle 220 38 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 220 39 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 220 40 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 220 41 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 220 42 Hofmann Hofmann NNP work_infoczas7nfbzac5mhjnxpkxle 220 43 et et NNP work_infoczas7nfbzac5mhjnxpkxle 220 44 al al NNP work_infoczas7nfbzac5mhjnxpkxle 220 45 . . . work_infoczas7nfbzac5mhjnxpkxle 221 1 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 221 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 221 3 and and CC work_infoczas7nfbzac5mhjnxpkxle 221 4 37 37 CD work_infoczas7nfbzac5mhjnxpkxle 221 5 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 221 6 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 221 7 from from IN work_infoczas7nfbzac5mhjnxpkxle 221 8 a a DT work_infoczas7nfbzac5mhjnxpkxle 221 9 total total NN work_infoczas7nfbzac5mhjnxpkxle 221 10 of of IN work_infoczas7nfbzac5mhjnxpkxle 221 11 120 120 CD work_infoczas7nfbzac5mhjnxpkxle 221 12 zygotes zygote NNS work_infoczas7nfbzac5mhjnxpkxle 221 13 treated treat VBN work_infoczas7nfbzac5mhjnxpkxle 221 14 with with IN work_infoczas7nfbzac5mhjnxpkxle 221 15 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 221 16 based base VBN work_infoczas7nfbzac5mhjnxpkxle 221 17 on on IN work_infoczas7nfbzac5mhjnxpkxle 221 18 equine equine NN work_infoczas7nfbzac5mhjnxpkxle 221 19 infectious infectious JJ work_infoczas7nfbzac5mhjnxpkxle 221 20 anaemia anaemia NN work_infoczas7nfbzac5mhjnxpkxle 221 21 virus virus NN work_infoczas7nfbzac5mhjnxpkxle 221 22 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 221 23 Whitelaw Whitelaw NNP work_infoczas7nfbzac5mhjnxpkxle 221 24 et et NNP work_infoczas7nfbzac5mhjnxpkxle 221 25 al al NNP work_infoczas7nfbzac5mhjnxpkxle 221 26 . . . work_infoczas7nfbzac5mhjnxpkxle 222 1 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 222 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 222 3 . . . work_infoczas7nfbzac5mhjnxpkxle 223 1 Notably notably RB work_infoczas7nfbzac5mhjnxpkxle 223 2 , , , work_infoczas7nfbzac5mhjnxpkxle 223 3 a a DT work_infoczas7nfbzac5mhjnxpkxle 223 4 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 223 5 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 223 6 based base VBN work_infoczas7nfbzac5mhjnxpkxle 223 7 approach approach NN work_infoczas7nfbzac5mhjnxpkxle 223 8 does do VBZ work_infoczas7nfbzac5mhjnxpkxle 223 9 not not RB work_infoczas7nfbzac5mhjnxpkxle 223 10 require require VB work_infoczas7nfbzac5mhjnxpkxle 223 11 classified classified JJ work_infoczas7nfbzac5mhjnxpkxle 223 12 laboratory laboratory NN work_infoczas7nfbzac5mhjnxpkxle 223 13 facilities facility NNS work_infoczas7nfbzac5mhjnxpkxle 223 14 for for IN work_infoczas7nfbzac5mhjnxpkxle 223 15 genetic genetic JJ work_infoczas7nfbzac5mhjnxpkxle 223 16 engineering engineering NN work_infoczas7nfbzac5mhjnxpkxle 223 17 of of IN work_infoczas7nfbzac5mhjnxpkxle 223 18 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 223 19 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 223 20 , , , work_infoczas7nfbzac5mhjnxpkxle 223 21 production production NN work_infoczas7nfbzac5mhjnxpkxle 223 22 of of IN work_infoczas7nfbzac5mhjnxpkxle 223 23 early early JJ work_infoczas7nfbzac5mhjnxpkxle 223 24 embryos embryo NNS work_infoczas7nfbzac5mhjnxpkxle 223 25 , , , work_infoczas7nfbzac5mhjnxpkxle 223 26 or or CC work_infoczas7nfbzac5mhjnxpkxle 223 27 housing housing NN work_infoczas7nfbzac5mhjnxpkxle 223 28 of of IN work_infoczas7nfbzac5mhjnxpkxle 223 29 the the DT work_infoczas7nfbzac5mhjnxpkxle 223 30 animals animal NNS work_infoczas7nfbzac5mhjnxpkxle 223 31 . . . work_infoczas7nfbzac5mhjnxpkxle 224 1 Our -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 224 2 protocol protocol NN work_infoczas7nfbzac5mhjnxpkxle 224 3 may may MD work_infoczas7nfbzac5mhjnxpkxle 224 4 be be VB work_infoczas7nfbzac5mhjnxpkxle 224 5 further further RB work_infoczas7nfbzac5mhjnxpkxle 224 6 refined refine VBN work_infoczas7nfbzac5mhjnxpkxle 224 7 for for IN work_infoczas7nfbzac5mhjnxpkxle 224 8 example example NN work_infoczas7nfbzac5mhjnxpkxle 224 9 by by IN work_infoczas7nfbzac5mhjnxpkxle 224 10 use use NN work_infoczas7nfbzac5mhjnxpkxle 224 11 of of IN work_infoczas7nfbzac5mhjnxpkxle 224 12 the the DT work_infoczas7nfbzac5mhjnxpkxle 224 13 highly highly RB work_infoczas7nfbzac5mhjnxpkxle 224 14 potent potent JJ work_infoczas7nfbzac5mhjnxpkxle 224 15 SB100X sb100x JJ work_infoczas7nfbzac5mhjnxpkxle 224 16 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 224 17 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 224 18 Mates Mates NNPS work_infoczas7nfbzac5mhjnxpkxle 224 19 et et NNP work_infoczas7nfbzac5mhjnxpkxle 224 20 al al NNP work_infoczas7nfbzac5mhjnxpkxle 224 21 . . . work_infoczas7nfbzac5mhjnxpkxle 225 1 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 225 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 225 3 encoded encode VBN work_infoczas7nfbzac5mhjnxpkxle 225 4 by by IN work_infoczas7nfbzac5mhjnxpkxle 225 5 trace trace NN work_infoczas7nfbzac5mhjnxpkxle 225 6 amounts amount NNS work_infoczas7nfbzac5mhjnxpkxle 225 7 of of IN work_infoczas7nfbzac5mhjnxpkxle 225 8 transfected transfected JJ work_infoczas7nfbzac5mhjnxpkxle 225 9 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 225 10 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 225 11 or or CC work_infoczas7nfbzac5mhjnxpkxle 225 12 in in IN work_infoczas7nfbzac5mhjnxpkxle 225 13 vitro vitro FW work_infoczas7nfbzac5mhjnxpkxle 225 14 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 225 15 transcribed transcribed NNP work_infoczas7nfbzac5mhjnxpkxle 225 16 RNA RNA NNP work_infoczas7nfbzac5mhjnxpkxle 225 17 . . . work_infoczas7nfbzac5mhjnxpkxle 226 1 We -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 226 2 believe believe VBP work_infoczas7nfbzac5mhjnxpkxle 226 3 that that IN work_infoczas7nfbzac5mhjnxpkxle 226 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 226 5 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 226 6 system system NN work_infoczas7nfbzac5mhjnxpkxle 226 7 combined combine VBN work_infoczas7nfbzac5mhjnxpkxle 226 8 with with IN work_infoczas7nfbzac5mhjnxpkxle 226 9 SCNT SCNT NNP work_infoczas7nfbzac5mhjnxpkxle 226 10 has have VBZ work_infoczas7nfbzac5mhjnxpkxle 226 11 the the DT work_infoczas7nfbzac5mhjnxpkxle 226 12 potential potential NN work_infoczas7nfbzac5mhjnxpkxle 226 13 of of IN work_infoczas7nfbzac5mhjnxpkxle 226 14 becoming become VBG work_infoczas7nfbzac5mhjnxpkxle 226 15 a a DT work_infoczas7nfbzac5mhjnxpkxle 226 16 preferred preferred JJ work_infoczas7nfbzac5mhjnxpkxle 226 17 tool tool NN work_infoczas7nfbzac5mhjnxpkxle 226 18 for for IN work_infoczas7nfbzac5mhjnxpkxle 226 19 introduction introduction NN work_infoczas7nfbzac5mhjnxpkxle 226 20 of of IN work_infoczas7nfbzac5mhjnxpkxle 226 21 dominant dominant JJ work_infoczas7nfbzac5mhjnxpkxle 226 22 mutations mutation NNS work_infoczas7nfbzac5mhjnxpkxle 226 23 or or CC work_infoczas7nfbzac5mhjnxpkxle 226 24 shRNA shrna NN work_infoczas7nfbzac5mhjnxpkxle 226 25 genes gene NNS work_infoczas7nfbzac5mhjnxpkxle 226 26 in in IN work_infoczas7nfbzac5mhjnxpkxle 226 27 the the DT work_infoczas7nfbzac5mhjnxpkxle 226 28 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 226 29 genome genome NN work_infoczas7nfbzac5mhjnxpkxle 226 30 and and CC work_infoczas7nfbzac5mhjnxpkxle 226 31 that that IN work_infoczas7nfbzac5mhjnxpkxle 226 32 this this DT work_infoczas7nfbzac5mhjnxpkxle 226 33 approach approach NN work_infoczas7nfbzac5mhjnxpkxle 226 34 will will MD work_infoczas7nfbzac5mhjnxpkxle 226 35 prove prove VB work_infoczas7nfbzac5mhjnxpkxle 226 36 to to TO work_infoczas7nfbzac5mhjnxpkxle 226 37 be be VB work_infoczas7nfbzac5mhjnxpkxle 226 38 of of IN work_infoczas7nfbzac5mhjnxpkxle 226 39 high high JJ work_infoczas7nfbzac5mhjnxpkxle 226 40 value value NN work_infoczas7nfbzac5mhjnxpkxle 226 41 for for IN work_infoczas7nfbzac5mhjnxpkxle 226 42 creation creation NN work_infoczas7nfbzac5mhjnxpkxle 226 43 of of IN work_infoczas7nfbzac5mhjnxpkxle 226 44 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 226 45 models model NNS work_infoczas7nfbzac5mhjnxpkxle 226 46 in in IN work_infoczas7nfbzac5mhjnxpkxle 226 47 the the DT work_infoczas7nfbzac5mhjnxpkxle 226 48 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 226 49 . . . work_infoczas7nfbzac5mhjnxpkxle 227 1 14 14 CD work_infoczas7nfbzac5mhjnxpkxle 227 2 MATERIALS material NNS work_infoczas7nfbzac5mhjnxpkxle 227 3 AND and CC work_infoczas7nfbzac5mhjnxpkxle 227 4 METHODS methods JJ work_infoczas7nfbzac5mhjnxpkxle 227 5 Vector vector NN work_infoczas7nfbzac5mhjnxpkxle 227 6 construction construction NN work_infoczas7nfbzac5mhjnxpkxle 227 7 The the DT work_infoczas7nfbzac5mhjnxpkxle 227 8 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 227 9 donor donor NN work_infoczas7nfbzac5mhjnxpkxle 227 10 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 227 11 , , , work_infoczas7nfbzac5mhjnxpkxle 227 12 pSBT pSBT NNP work_infoczas7nfbzac5mhjnxpkxle 227 13 / / SYM work_infoczas7nfbzac5mhjnxpkxle 227 14 cHS4.Ubi cHS4.Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 227 15 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 227 16 GIN.cHS4 GIN.cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 227 17 , , , work_infoczas7nfbzac5mhjnxpkxle 227 18 was be VBD work_infoczas7nfbzac5mhjnxpkxle 227 19 generated generate VBN work_infoczas7nfbzac5mhjnxpkxle 227 20 by by IN work_infoczas7nfbzac5mhjnxpkxle 227 21 inserting insert VBG work_infoczas7nfbzac5mhjnxpkxle 227 22 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 227 23 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 227 24 amplified amplify VBN work_infoczas7nfbzac5mhjnxpkxle 227 25 1.2-kb 1.2-kb CD work_infoczas7nfbzac5mhjnxpkxle 227 26 cHS4 chs4 IN work_infoczas7nfbzac5mhjnxpkxle 227 27 insulators insulator NNS work_infoczas7nfbzac5mhjnxpkxle 227 28 into into IN work_infoczas7nfbzac5mhjnxpkxle 227 29 linkers linker NNS work_infoczas7nfbzac5mhjnxpkxle 227 30 flanking flank VBG work_infoczas7nfbzac5mhjnxpkxle 227 31 the the DT work_infoczas7nfbzac5mhjnxpkxle 227 32 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 227 33 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 227 34 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 227 35 in in IN work_infoczas7nfbzac5mhjnxpkxle 227 36 the the DT work_infoczas7nfbzac5mhjnxpkxle 227 37 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 227 38 pSBT psbt NN work_infoczas7nfbzac5mhjnxpkxle 227 39 / / SYM work_infoczas7nfbzac5mhjnxpkxle 227 40 Ubi Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 227 41 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 227 42 GIN GIN NNP work_infoczas7nfbzac5mhjnxpkxle 227 43 . . . work_infoczas7nfbzac5mhjnxpkxle 228 1 This this DT work_infoczas7nfbzac5mhjnxpkxle 228 2 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 228 3 contains contain VBZ work_infoczas7nfbzac5mhjnxpkxle 228 4 a a DT work_infoczas7nfbzac5mhjnxpkxle 228 5 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 228 6 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 228 7 IRES IRES NNP work_infoczas7nfbzac5mhjnxpkxle 228 8 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 228 9 neo neo NNP work_infoczas7nfbzac5mhjnxpkxle 228 10 r r NNP work_infoczas7nfbzac5mhjnxpkxle 228 11 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 228 12 GIN GIN NNP work_infoczas7nfbzac5mhjnxpkxle 228 13 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 228 14 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 228 15 driven drive VBN work_infoczas7nfbzac5mhjnxpkxle 228 16 by by IN work_infoczas7nfbzac5mhjnxpkxle 228 17 the the DT work_infoczas7nfbzac5mhjnxpkxle 228 18 human human NN work_infoczas7nfbzac5mhjnxpkxle 228 19 ubiquitin ubiquitin JJ work_infoczas7nfbzac5mhjnxpkxle 228 20 C C NNP work_infoczas7nfbzac5mhjnxpkxle 228 21 promoter promoter NN work_infoczas7nfbzac5mhjnxpkxle 228 22 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 228 23 Ubi ubi NN work_infoczas7nfbzac5mhjnxpkxle 228 24 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 228 25 and and CC work_infoczas7nfbzac5mhjnxpkxle 228 26 was be VBD work_infoczas7nfbzac5mhjnxpkxle 228 27 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 228 28 from from IN work_infoczas7nfbzac5mhjnxpkxle 228 29 pSBT psbt JJ work_infoczas7nfbzac5mhjnxpkxle 228 30 / / SYM work_infoczas7nfbzac5mhjnxpkxle 228 31 RSV RSV NNP work_infoczas7nfbzac5mhjnxpkxle 228 32 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 228 33 FGIP FGIP NNP work_infoczas7nfbzac5mhjnxpkxle 228 34 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 228 35 Moldt Moldt NNP work_infoczas7nfbzac5mhjnxpkxle 228 36 et et NNP work_infoczas7nfbzac5mhjnxpkxle 228 37 al al NNP work_infoczas7nfbzac5mhjnxpkxle 228 38 . . . work_infoczas7nfbzac5mhjnxpkxle 229 1 2008 2008 LS work_infoczas7nfbzac5mhjnxpkxle 229 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 229 3 . . . work_infoczas7nfbzac5mhjnxpkxle 230 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 230 2 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 230 3 sequence sequence NN work_infoczas7nfbzac5mhjnxpkxle 230 4 , , , work_infoczas7nfbzac5mhjnxpkxle 230 5 originally originally RB work_infoczas7nfbzac5mhjnxpkxle 230 6 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 230 7 from from IN work_infoczas7nfbzac5mhjnxpkxle 230 8 peGFP.N1 pegfp.n1 PRP work_infoczas7nfbzac5mhjnxpkxle 230 9 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 230 10 Clontech Clontech NNP work_infoczas7nfbzac5mhjnxpkxle 230 11 , , , work_infoczas7nfbzac5mhjnxpkxle 230 12 Mountain Mountain NNP work_infoczas7nfbzac5mhjnxpkxle 230 13 View View NNP work_infoczas7nfbzac5mhjnxpkxle 230 14 , , , work_infoczas7nfbzac5mhjnxpkxle 230 15 CA CA NNP work_infoczas7nfbzac5mhjnxpkxle 230 16 , , , work_infoczas7nfbzac5mhjnxpkxle 230 17 USA USA NNP work_infoczas7nfbzac5mhjnxpkxle 230 18 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 230 19 , , , work_infoczas7nfbzac5mhjnxpkxle 230 20 contained contain VBD work_infoczas7nfbzac5mhjnxpkxle 230 21 a a DT work_infoczas7nfbzac5mhjnxpkxle 230 22 Flp Flp NNP work_infoczas7nfbzac5mhjnxpkxle 230 23 recognition recognition NN work_infoczas7nfbzac5mhjnxpkxle 230 24 sequence sequence NN work_infoczas7nfbzac5mhjnxpkxle 230 25 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 230 26 FRT FRT NNP work_infoczas7nfbzac5mhjnxpkxle 230 27 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 230 28 inserted insert VBD work_infoczas7nfbzac5mhjnxpkxle 230 29 between between IN work_infoczas7nfbzac5mhjnxpkxle 230 30 the the DT work_infoczas7nfbzac5mhjnxpkxle 230 31 start start NN work_infoczas7nfbzac5mhjnxpkxle 230 32 codon codon NNP work_infoczas7nfbzac5mhjnxpkxle 230 33 and and CC work_infoczas7nfbzac5mhjnxpkxle 230 34 the the DT work_infoczas7nfbzac5mhjnxpkxle 230 35 remaining remain VBG work_infoczas7nfbzac5mhjnxpkxle 230 36 part part NN work_infoczas7nfbzac5mhjnxpkxle 230 37 of of IN work_infoczas7nfbzac5mhjnxpkxle 230 38 the the DT work_infoczas7nfbzac5mhjnxpkxle 230 39 eGFP egfp NN work_infoczas7nfbzac5mhjnxpkxle 230 40 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 230 41 , , , work_infoczas7nfbzac5mhjnxpkxle 230 42 as as IN work_infoczas7nfbzac5mhjnxpkxle 230 43 described describe VBN work_infoczas7nfbzac5mhjnxpkxle 230 44 in in IN work_infoczas7nfbzac5mhjnxpkxle 230 45 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 230 46 Moldt Moldt NNP work_infoczas7nfbzac5mhjnxpkxle 230 47 et et NNP work_infoczas7nfbzac5mhjnxpkxle 230 48 al al NNP work_infoczas7nfbzac5mhjnxpkxle 230 49 . . . work_infoczas7nfbzac5mhjnxpkxle 231 1 2008 2008 LS work_infoczas7nfbzac5mhjnxpkxle 231 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 231 3 . . . work_infoczas7nfbzac5mhjnxpkxle 232 1 This this DT work_infoczas7nfbzac5mhjnxpkxle 232 2 chimeric chimeric JJ work_infoczas7nfbzac5mhjnxpkxle 232 3 variant variant NN work_infoczas7nfbzac5mhjnxpkxle 232 4 of of IN work_infoczas7nfbzac5mhjnxpkxle 232 5 the the DT work_infoczas7nfbzac5mhjnxpkxle 232 6 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 232 7 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 232 8 here here RB work_infoczas7nfbzac5mhjnxpkxle 232 9 simply simply RB work_infoczas7nfbzac5mhjnxpkxle 232 10 designated designate VBN work_infoczas7nfbzac5mhjnxpkxle 232 11 ‘ ' `` work_infoczas7nfbzac5mhjnxpkxle 232 12 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 232 13 ’ ' '' work_infoczas7nfbzac5mhjnxpkxle 232 14 . . . work_infoczas7nfbzac5mhjnxpkxle 233 1 pSBT pSBT NNP work_infoczas7nfbzac5mhjnxpkxle 233 2 / / SYM work_infoczas7nfbzac5mhjnxpkxle 233 3 Ubi Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 233 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 233 5 GIN GIN NNP work_infoczas7nfbzac5mhjnxpkxle 233 6 was be VBD work_infoczas7nfbzac5mhjnxpkxle 233 7 generated generate VBN work_infoczas7nfbzac5mhjnxpkxle 233 8 by by IN work_infoczas7nfbzac5mhjnxpkxle 233 9 replacing replace VBG work_infoczas7nfbzac5mhjnxpkxle 233 10 the the DT work_infoczas7nfbzac5mhjnxpkxle 233 11 SV40 SV40 NNP work_infoczas7nfbzac5mhjnxpkxle 233 12 promoter promoter NN work_infoczas7nfbzac5mhjnxpkxle 233 13 of of IN work_infoczas7nfbzac5mhjnxpkxle 233 14 pSBT psbt NN work_infoczas7nfbzac5mhjnxpkxle 233 15 / / SYM work_infoczas7nfbzac5mhjnxpkxle 233 16 SV40-FGIP SV40-FGIP NNP work_infoczas7nfbzac5mhjnxpkxle 233 17 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 233 18 similar similar JJ work_infoczas7nfbzac5mhjnxpkxle 233 19 , , , work_infoczas7nfbzac5mhjnxpkxle 233 20 except except IN work_infoczas7nfbzac5mhjnxpkxle 233 21 for for IN work_infoczas7nfbzac5mhjnxpkxle 233 22 the the DT work_infoczas7nfbzac5mhjnxpkxle 233 23 promoter promoter NN work_infoczas7nfbzac5mhjnxpkxle 233 24 , , , work_infoczas7nfbzac5mhjnxpkxle 233 25 to to IN work_infoczas7nfbzac5mhjnxpkxle 233 26 pSBT psbt JJ work_infoczas7nfbzac5mhjnxpkxle 233 27 / / SYM work_infoczas7nfbzac5mhjnxpkxle 233 28 RSV RSV NNP work_infoczas7nfbzac5mhjnxpkxle 233 29 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 233 30 FGIP FGIP NNP work_infoczas7nfbzac5mhjnxpkxle 233 31 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 233 32 with with IN work_infoczas7nfbzac5mhjnxpkxle 233 33 the the DT work_infoczas7nfbzac5mhjnxpkxle 233 34 human human JJ work_infoczas7nfbzac5mhjnxpkxle 233 35 ubiquitin ubiquitin JJ work_infoczas7nfbzac5mhjnxpkxle 233 36 C C NNP work_infoczas7nfbzac5mhjnxpkxle 233 37 promoter promoter NN work_infoczas7nfbzac5mhjnxpkxle 233 38 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 233 39 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 233 40 amplified amplify VBN work_infoczas7nfbzac5mhjnxpkxle 233 41 from from IN work_infoczas7nfbzac5mhjnxpkxle 233 42 pUbiC pubic NN work_infoczas7nfbzac5mhjnxpkxle 233 43 - - : work_infoczas7nfbzac5mhjnxpkxle 233 44 Jun Jun NNP work_infoczas7nfbzac5mhjnxpkxle 233 45 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 233 46 Schorpp Schorpp NNP work_infoczas7nfbzac5mhjnxpkxle 233 47 et et FW work_infoczas7nfbzac5mhjnxpkxle 233 48 al al NNP work_infoczas7nfbzac5mhjnxpkxle 233 49 . . . work_infoczas7nfbzac5mhjnxpkxle 234 1 1996 1996 CD work_infoczas7nfbzac5mhjnxpkxle 234 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 234 3 using use VBG work_infoczas7nfbzac5mhjnxpkxle 234 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 234 5 following follow VBG work_infoczas7nfbzac5mhjnxpkxle 234 6 two two CD work_infoczas7nfbzac5mhjnxpkxle 234 7 primers primer NNS work_infoczas7nfbzac5mhjnxpkxle 234 8 : : : work_infoczas7nfbzac5mhjnxpkxle 234 9 5´- 5´- CD work_infoczas7nfbzac5mhjnxpkxle 234 10 GTAAACGCGTAGATCTGGCCTCCGCGCC-3 GTAAACGCGTAGATCTGGCCTCCGCGCC-3 NNP work_infoczas7nfbzac5mhjnxpkxle 234 11 ´ ´ CD work_infoczas7nfbzac5mhjnxpkxle 234 12 and and CC work_infoczas7nfbzac5mhjnxpkxle 234 13 5’-CGGTCCATGGAAGCTTCGTCTAACAAAAAAG-3 5’-CGGTCCATGGAAGCTTCGTCTAACAAAAAAG-3 NNP work_infoczas7nfbzac5mhjnxpkxle 234 14 ’ ' '' work_infoczas7nfbzac5mhjnxpkxle 234 15 . . . work_infoczas7nfbzac5mhjnxpkxle 235 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 235 2 fragment fragment NN work_infoczas7nfbzac5mhjnxpkxle 235 3 was be VBD work_infoczas7nfbzac5mhjnxpkxle 235 4 inserted insert VBN work_infoczas7nfbzac5mhjnxpkxle 235 5 into into IN work_infoczas7nfbzac5mhjnxpkxle 235 6 MluI MluI NNP work_infoczas7nfbzac5mhjnxpkxle 235 7 / / SYM work_infoczas7nfbzac5mhjnxpkxle 235 8 NcoI NcoI NNP work_infoczas7nfbzac5mhjnxpkxle 235 9 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 235 10 digested digest VBN work_infoczas7nfbzac5mhjnxpkxle 235 11 pSBT psbt NN work_infoczas7nfbzac5mhjnxpkxle 235 12 / / SYM work_infoczas7nfbzac5mhjnxpkxle 235 13 SV40-FGIP SV40-FGIP NNP work_infoczas7nfbzac5mhjnxpkxle 235 14 . . . work_infoczas7nfbzac5mhjnxpkxle 236 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 236 2 IRES ires JJ work_infoczas7nfbzac5mhjnxpkxle 236 3 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 236 4 puro puro NN work_infoczas7nfbzac5mhjnxpkxle 236 5 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 236 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 236 7 pSBT psbt NN work_infoczas7nfbzac5mhjnxpkxle 236 8 / / SYM work_infoczas7nfbzac5mhjnxpkxle 236 9 Ubi- Ubi- NNP work_infoczas7nfbzac5mhjnxpkxle 236 10 FGIP FGIP NNP work_infoczas7nfbzac5mhjnxpkxle 236 11 was be VBD work_infoczas7nfbzac5mhjnxpkxle 236 12 replaced replace VBN work_infoczas7nfbzac5mhjnxpkxle 236 13 with with IN work_infoczas7nfbzac5mhjnxpkxle 236 14 an an DT work_infoczas7nfbzac5mhjnxpkxle 236 15 IRES IRES NNP work_infoczas7nfbzac5mhjnxpkxle 236 16 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 236 17 neo neo NN work_infoczas7nfbzac5mhjnxpkxle 236 18 r r NN work_infoczas7nfbzac5mhjnxpkxle 236 19 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 236 20 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 236 21 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 236 22 amplified amplify VBN work_infoczas7nfbzac5mhjnxpkxle 236 23 from from IN work_infoczas7nfbzac5mhjnxpkxle 236 24 pCMV pcmv NN work_infoczas7nfbzac5mhjnxpkxle 236 25 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 236 26 Bipep Bipep NNP work_infoczas7nfbzac5mhjnxpkxle 236 27 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 236 28 Tolstrup Tolstrup NNP work_infoczas7nfbzac5mhjnxpkxle 236 29 et et FW work_infoczas7nfbzac5mhjnxpkxle 236 30 al al NNP work_infoczas7nfbzac5mhjnxpkxle 236 31 . . . work_infoczas7nfbzac5mhjnxpkxle 237 1 2001 2001 LS work_infoczas7nfbzac5mhjnxpkxle 237 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 237 3 creating create VBG work_infoczas7nfbzac5mhjnxpkxle 237 4 pSBT psbt NN work_infoczas7nfbzac5mhjnxpkxle 237 5 / / SYM work_infoczas7nfbzac5mhjnxpkxle 237 6 Ubi Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 237 7 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 237 8 GIN GIN NNP work_infoczas7nfbzac5mhjnxpkxle 237 9 . . . work_infoczas7nfbzac5mhjnxpkxle 238 1 pCMV pCMV NNP work_infoczas7nfbzac5mhjnxpkxle 238 2 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 238 3 HSB3 HSB3 NNP work_infoczas7nfbzac5mhjnxpkxle 238 4 was be VBD work_infoczas7nfbzac5mhjnxpkxle 238 5 previously previously RB work_infoczas7nfbzac5mhjnxpkxle 238 6 described describe VBN work_infoczas7nfbzac5mhjnxpkxle 238 7 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 238 8 Yant Yant NNP work_infoczas7nfbzac5mhjnxpkxle 238 9 et et FW work_infoczas7nfbzac5mhjnxpkxle 238 10 al al NNP work_infoczas7nfbzac5mhjnxpkxle 238 11 . . . work_infoczas7nfbzac5mhjnxpkxle 239 1 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 239 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 239 3 . . . work_infoczas7nfbzac5mhjnxpkxle 240 1 Generation generation NN work_infoczas7nfbzac5mhjnxpkxle 240 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 240 3 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 240 4 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 240 5 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 240 6 by by IN work_infoczas7nfbzac5mhjnxpkxle 240 7 transfection transfection NN work_infoczas7nfbzac5mhjnxpkxle 240 8 of of IN work_infoczas7nfbzac5mhjnxpkxle 240 9 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 240 10 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 240 11 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 240 12 Neonatal Neonatal NNP work_infoczas7nfbzac5mhjnxpkxle 240 13 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 240 14 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 240 15 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 240 16 NPFs NPFs NNP work_infoczas7nfbzac5mhjnxpkxle 240 17 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 240 18 were be VBD work_infoczas7nfbzac5mhjnxpkxle 240 19 cultured culture VBN work_infoczas7nfbzac5mhjnxpkxle 240 20 from from IN work_infoczas7nfbzac5mhjnxpkxle 240 21 ear ear NN work_infoczas7nfbzac5mhjnxpkxle 240 22 biopsies biopsy NNS work_infoczas7nfbzac5mhjnxpkxle 240 23 of of IN work_infoczas7nfbzac5mhjnxpkxle 240 24 newborn newborn JJ work_infoczas7nfbzac5mhjnxpkxle 240 25 Göttingen Göttingen NNP work_infoczas7nfbzac5mhjnxpkxle 240 26 minipig minipig NNP work_infoczas7nfbzac5mhjnxpkxle 240 27 no no UH work_infoczas7nfbzac5mhjnxpkxle 240 28 . . . work_infoczas7nfbzac5mhjnxpkxle 241 1 74113 74113 CD work_infoczas7nfbzac5mhjnxpkxle 241 2 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 241 3 Ellegaard Ellegaard NNP work_infoczas7nfbzac5mhjnxpkxle 241 4 Göttingen Göttingen NNP work_infoczas7nfbzac5mhjnxpkxle 241 5 Minipigs Minipigs NNP work_infoczas7nfbzac5mhjnxpkxle 241 6 A A NNP work_infoczas7nfbzac5mhjnxpkxle 241 7 / / SYM work_infoczas7nfbzac5mhjnxpkxle 241 8 S s NN work_infoczas7nfbzac5mhjnxpkxle 241 9 , , , work_infoczas7nfbzac5mhjnxpkxle 241 10 Soroe Soroe NNP work_infoczas7nfbzac5mhjnxpkxle 241 11 Landevej Landevej NNP work_infoczas7nfbzac5mhjnxpkxle 241 12 302 302 CD work_infoczas7nfbzac5mhjnxpkxle 241 13 , , , work_infoczas7nfbzac5mhjnxpkxle 241 14 DK-4261 DK-4261 NNPS work_infoczas7nfbzac5mhjnxpkxle 241 15 Dalmose Dalmose NNP work_infoczas7nfbzac5mhjnxpkxle 241 16 , , , work_infoczas7nfbzac5mhjnxpkxle 241 17 Denmark Denmark NNP work_infoczas7nfbzac5mhjnxpkxle 241 18 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 241 19 as as IN work_infoczas7nfbzac5mhjnxpkxle 241 20 previously previously RB work_infoczas7nfbzac5mhjnxpkxle 241 21 described describe VBN work_infoczas7nfbzac5mhjnxpkxle 241 22 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 241 23 Kragh Kragh NNP work_infoczas7nfbzac5mhjnxpkxle 241 24 et et FW work_infoczas7nfbzac5mhjnxpkxle 241 25 al al NNP work_infoczas7nfbzac5mhjnxpkxle 241 26 . . . work_infoczas7nfbzac5mhjnxpkxle 242 1 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 242 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 242 3 . . . work_infoczas7nfbzac5mhjnxpkxle 243 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 243 2 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 243 3 were be VBD work_infoczas7nfbzac5mhjnxpkxle 243 4 grown grow VBN work_infoczas7nfbzac5mhjnxpkxle 243 5 in in IN work_infoczas7nfbzac5mhjnxpkxle 243 6 DMEM DMEM NNS work_infoczas7nfbzac5mhjnxpkxle 243 7 to to IN work_infoczas7nfbzac5mhjnxpkxle 243 8 50 50 CD work_infoczas7nfbzac5mhjnxpkxle 243 9 % % NN work_infoczas7nfbzac5mhjnxpkxle 243 10 confluence confluence NN work_infoczas7nfbzac5mhjnxpkxle 243 11 , , , work_infoczas7nfbzac5mhjnxpkxle 243 12 trypsinized trypsinize VBN work_infoczas7nfbzac5mhjnxpkxle 243 13 , , , work_infoczas7nfbzac5mhjnxpkxle 243 14 and and CC work_infoczas7nfbzac5mhjnxpkxle 243 15 resuspended resuspend VBD work_infoczas7nfbzac5mhjnxpkxle 243 16 for for IN work_infoczas7nfbzac5mhjnxpkxle 243 17 further further JJ work_infoczas7nfbzac5mhjnxpkxle 243 18 expansion expansion NN work_infoczas7nfbzac5mhjnxpkxle 243 19 prior prior RB work_infoczas7nfbzac5mhjnxpkxle 243 20 to to IN work_infoczas7nfbzac5mhjnxpkxle 243 21 freezing freezing NN work_infoczas7nfbzac5mhjnxpkxle 243 22 of of IN work_infoczas7nfbzac5mhjnxpkxle 243 23 aliquots aliquot NNS work_infoczas7nfbzac5mhjnxpkxle 243 24 . . . work_infoczas7nfbzac5mhjnxpkxle 244 1 For for IN work_infoczas7nfbzac5mhjnxpkxle 244 2 production production NN work_infoczas7nfbzac5mhjnxpkxle 244 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 244 4 a a DT work_infoczas7nfbzac5mhjnxpkxle 244 5 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 244 6 litter litter NN work_infoczas7nfbzac5mhjnxpkxle 244 7 , , , work_infoczas7nfbzac5mhjnxpkxle 244 8 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 244 9   CD work_infoczas7nfbzac5mhjnxpkxle 244 10 10 10 CD work_infoczas7nfbzac5mhjnxpkxle 244 11 5 5 CD work_infoczas7nfbzac5mhjnxpkxle 244 12 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 244 13 were be VBD work_infoczas7nfbzac5mhjnxpkxle 244 14 transfected transfecte VBN work_infoczas7nfbzac5mhjnxpkxle 244 15 in in IN work_infoczas7nfbzac5mhjnxpkxle 244 16 a a DT work_infoczas7nfbzac5mhjnxpkxle 244 17 in in IN work_infoczas7nfbzac5mhjnxpkxle 244 18 a a DT work_infoczas7nfbzac5mhjnxpkxle 244 19 60-cm 60-cm $ work_infoczas7nfbzac5mhjnxpkxle 244 20 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 244 21 dish dish NN work_infoczas7nfbzac5mhjnxpkxle 244 22 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 244 23 in in IN work_infoczas7nfbzac5mhjnxpkxle 244 24 10 10 CD work_infoczas7nfbzac5mhjnxpkxle 244 25 ml ml NN work_infoczas7nfbzac5mhjnxpkxle 244 26 medium medium NN work_infoczas7nfbzac5mhjnxpkxle 244 27 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 244 28 using use VBG work_infoczas7nfbzac5mhjnxpkxle 244 29 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 244 30 µg µg IN work_infoczas7nfbzac5mhjnxpkxle 244 31 of of IN work_infoczas7nfbzac5mhjnxpkxle 244 32 the the DT work_infoczas7nfbzac5mhjnxpkxle 244 33 pSBT psbt NN work_infoczas7nfbzac5mhjnxpkxle 244 34 / / SYM work_infoczas7nfbzac5mhjnxpkxle 244 35 cHS4.Ubi- cHS4.Ubi- NNP work_infoczas7nfbzac5mhjnxpkxle 244 36 GIN.cHS4 GIN.cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 244 37 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 244 38 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 244 39 and and CC work_infoczas7nfbzac5mhjnxpkxle 244 40 50 50 CD work_infoczas7nfbzac5mhjnxpkxle 244 41 ng ng NN work_infoczas7nfbzac5mhjnxpkxle 244 42 pCMV pcmv JJ work_infoczas7nfbzac5mhjnxpkxle 244 43 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 244 44 HSB3 HSB3 NNP work_infoczas7nfbzac5mhjnxpkxle 244 45 or or CC work_infoczas7nfbzac5mhjnxpkxle 244 46 as as IN work_infoczas7nfbzac5mhjnxpkxle 244 47 control control NN work_infoczas7nfbzac5mhjnxpkxle 244 48 50 50 CD work_infoczas7nfbzac5mhjnxpkxle 244 49 ng ng NN work_infoczas7nfbzac5mhjnxpkxle 244 50 pUC19 pUC19 NNP work_infoczas7nfbzac5mhjnxpkxle 244 51 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 244 52 . . . work_infoczas7nfbzac5mhjnxpkxle 245 1 10 10 CD work_infoczas7nfbzac5mhjnxpkxle 245 2 µl µl IN work_infoczas7nfbzac5mhjnxpkxle 245 3 FuGENE FuGENE NNP work_infoczas7nfbzac5mhjnxpkxle 245 4 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 245 5 15 15 CD work_infoczas7nfbzac5mhjnxpkxle 245 6 was be VBD work_infoczas7nfbzac5mhjnxpkxle 245 7 used use VBN work_infoczas7nfbzac5mhjnxpkxle 245 8 in in IN work_infoczas7nfbzac5mhjnxpkxle 245 9 the the DT work_infoczas7nfbzac5mhjnxpkxle 245 10 reaction reaction NN work_infoczas7nfbzac5mhjnxpkxle 245 11 mixture mixture NN work_infoczas7nfbzac5mhjnxpkxle 245 12 . . . work_infoczas7nfbzac5mhjnxpkxle 246 1 On on IN work_infoczas7nfbzac5mhjnxpkxle 246 2 the the DT work_infoczas7nfbzac5mhjnxpkxle 246 3 following follow VBG work_infoczas7nfbzac5mhjnxpkxle 246 4 day day NN work_infoczas7nfbzac5mhjnxpkxle 246 5 , , , work_infoczas7nfbzac5mhjnxpkxle 246 6 the the DT work_infoczas7nfbzac5mhjnxpkxle 246 7 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 246 8 were be VBD work_infoczas7nfbzac5mhjnxpkxle 246 9 washed wash VBN work_infoczas7nfbzac5mhjnxpkxle 246 10 with with IN work_infoczas7nfbzac5mhjnxpkxle 246 11 PBS PBS NNP work_infoczas7nfbzac5mhjnxpkxle 246 12 and and CC work_infoczas7nfbzac5mhjnxpkxle 246 13 subsequently subsequently RB work_infoczas7nfbzac5mhjnxpkxle 246 14 cultured cultured JJ work_infoczas7nfbzac5mhjnxpkxle 246 15 in in IN work_infoczas7nfbzac5mhjnxpkxle 246 16 G418-containing g418-containe VBG work_infoczas7nfbzac5mhjnxpkxle 246 17 medium medium NN work_infoczas7nfbzac5mhjnxpkxle 246 18 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 246 19 0.75 0.75 CD work_infoczas7nfbzac5mhjnxpkxle 246 20 µg µg IN work_infoczas7nfbzac5mhjnxpkxle 246 21 / / SYM work_infoczas7nfbzac5mhjnxpkxle 246 22 ml ml NN work_infoczas7nfbzac5mhjnxpkxle 246 23 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 246 24 for for IN work_infoczas7nfbzac5mhjnxpkxle 246 25 two two CD work_infoczas7nfbzac5mhjnxpkxle 246 26 weeks week NNS work_infoczas7nfbzac5mhjnxpkxle 246 27 . . . work_infoczas7nfbzac5mhjnxpkxle 247 1 A a DT work_infoczas7nfbzac5mhjnxpkxle 247 2 total total NN work_infoczas7nfbzac5mhjnxpkxle 247 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 247 4 80 80 CD work_infoczas7nfbzac5mhjnxpkxle 247 5 colonies colony NNS work_infoczas7nfbzac5mhjnxpkxle 247 6 were be VBD work_infoczas7nfbzac5mhjnxpkxle 247 7 pooled pool VBN work_infoczas7nfbzac5mhjnxpkxle 247 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 247 9 grown grow VBN work_infoczas7nfbzac5mhjnxpkxle 247 10 for for IN work_infoczas7nfbzac5mhjnxpkxle 247 11 9 9 CD work_infoczas7nfbzac5mhjnxpkxle 247 12 days day NNS work_infoczas7nfbzac5mhjnxpkxle 247 13 prior prior RB work_infoczas7nfbzac5mhjnxpkxle 247 14 to to IN work_infoczas7nfbzac5mhjnxpkxle 247 15 SCNT SCNT NNP work_infoczas7nfbzac5mhjnxpkxle 247 16 by by IN work_infoczas7nfbzac5mhjnxpkxle 247 17 HMC HMC NNP work_infoczas7nfbzac5mhjnxpkxle 247 18 . . . work_infoczas7nfbzac5mhjnxpkxle 248 1 Handmade handmade JJ work_infoczas7nfbzac5mhjnxpkxle 248 2 cloning cloning NN work_infoczas7nfbzac5mhjnxpkxle 248 3 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 248 4 HMC HMC NNP work_infoczas7nfbzac5mhjnxpkxle 248 5 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 248 6 using use VBG work_infoczas7nfbzac5mhjnxpkxle 248 7 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 248 8 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 248 9 treated treat VBN work_infoczas7nfbzac5mhjnxpkxle 248 10 NPFs NPFs NNP work_infoczas7nfbzac5mhjnxpkxle 248 11 Handmade Handmade NNP work_infoczas7nfbzac5mhjnxpkxle 248 12 cloning cloning NN work_infoczas7nfbzac5mhjnxpkxle 248 13 was be VBD work_infoczas7nfbzac5mhjnxpkxle 248 14 performed perform VBN work_infoczas7nfbzac5mhjnxpkxle 248 15 as as IN work_infoczas7nfbzac5mhjnxpkxle 248 16 previously previously RB work_infoczas7nfbzac5mhjnxpkxle 248 17 described describe VBN work_infoczas7nfbzac5mhjnxpkxle 248 18 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 248 19 Du Du NNP work_infoczas7nfbzac5mhjnxpkxle 248 20 et et NNP work_infoczas7nfbzac5mhjnxpkxle 248 21 al al NNP work_infoczas7nfbzac5mhjnxpkxle 248 22 . . . work_infoczas7nfbzac5mhjnxpkxle 249 1 2007 2007 CD work_infoczas7nfbzac5mhjnxpkxle 249 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 249 3 . . . work_infoczas7nfbzac5mhjnxpkxle 250 1 Briefly briefly RB work_infoczas7nfbzac5mhjnxpkxle 250 2 , , , work_infoczas7nfbzac5mhjnxpkxle 250 3 oocytes oocyte NNS work_infoczas7nfbzac5mhjnxpkxle 250 4 with with IN work_infoczas7nfbzac5mhjnxpkxle 250 5 partially partially RB work_infoczas7nfbzac5mhjnxpkxle 250 6 digested digest VBN work_infoczas7nfbzac5mhjnxpkxle 250 7 zonae zonae NNP work_infoczas7nfbzac5mhjnxpkxle 250 8 pellucidae pellucidae NN work_infoczas7nfbzac5mhjnxpkxle 250 9 were be VBD work_infoczas7nfbzac5mhjnxpkxle 250 10 enucleated enucleate VBN work_infoczas7nfbzac5mhjnxpkxle 250 11 by by IN work_infoczas7nfbzac5mhjnxpkxle 250 12 oriented orient VBN work_infoczas7nfbzac5mhjnxpkxle 250 13 bisection bisection NN work_infoczas7nfbzac5mhjnxpkxle 250 14 according accord VBG work_infoczas7nfbzac5mhjnxpkxle 250 15 to to IN work_infoczas7nfbzac5mhjnxpkxle 250 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 250 17 polar polar JJ work_infoczas7nfbzac5mhjnxpkxle 250 18 body body NN work_infoczas7nfbzac5mhjnxpkxle 250 19 position position NN work_infoczas7nfbzac5mhjnxpkxle 250 20 . . . work_infoczas7nfbzac5mhjnxpkxle 251 1 For for IN work_infoczas7nfbzac5mhjnxpkxle 251 2 each each DT work_infoczas7nfbzac5mhjnxpkxle 251 3 oocyte oocyte NN work_infoczas7nfbzac5mhjnxpkxle 251 4 , , , work_infoczas7nfbzac5mhjnxpkxle 251 5 the the DT work_infoczas7nfbzac5mhjnxpkxle 251 6 part part NN work_infoczas7nfbzac5mhjnxpkxle 251 7 without without IN work_infoczas7nfbzac5mhjnxpkxle 251 8 chromatin chromatin NNP work_infoczas7nfbzac5mhjnxpkxle 251 9 , , , work_infoczas7nfbzac5mhjnxpkxle 251 10 i.e. i.e. FW work_infoczas7nfbzac5mhjnxpkxle 252 1 the the DT work_infoczas7nfbzac5mhjnxpkxle 252 2 cytoplast cytoplast NN work_infoczas7nfbzac5mhjnxpkxle 252 3 , , , work_infoczas7nfbzac5mhjnxpkxle 252 4 was be VBD work_infoczas7nfbzac5mhjnxpkxle 252 5 collected collect VBN work_infoczas7nfbzac5mhjnxpkxle 252 6 and and CC work_infoczas7nfbzac5mhjnxpkxle 252 7 electrofused electrofuse VBN work_infoczas7nfbzac5mhjnxpkxle 252 8 with with IN work_infoczas7nfbzac5mhjnxpkxle 252 9 one one CD work_infoczas7nfbzac5mhjnxpkxle 252 10 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 252 11 fibroblast fibroblast NN work_infoczas7nfbzac5mhjnxpkxle 252 12 modified modify VBN work_infoczas7nfbzac5mhjnxpkxle 252 13 by by IN work_infoczas7nfbzac5mhjnxpkxle 252 14 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 252 15 to to TO work_infoczas7nfbzac5mhjnxpkxle 252 16 carry carry VB work_infoczas7nfbzac5mhjnxpkxle 252 17 the the DT work_infoczas7nfbzac5mhjnxpkxle 252 18 cHS4.Ubi cHS4.Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 252 19 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 252 20 GIN.cHS4 GIN.cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 252 21 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 252 22 . . . work_infoczas7nfbzac5mhjnxpkxle 253 1 Another another DT work_infoczas7nfbzac5mhjnxpkxle 253 2 cytoplast cytoplast NN work_infoczas7nfbzac5mhjnxpkxle 253 3 was be VBD work_infoczas7nfbzac5mhjnxpkxle 253 4 then then RB work_infoczas7nfbzac5mhjnxpkxle 253 5 electrofused electrofuse VBN work_infoczas7nfbzac5mhjnxpkxle 253 6 with with IN work_infoczas7nfbzac5mhjnxpkxle 253 7 each each DT work_infoczas7nfbzac5mhjnxpkxle 253 8 cytoplast cytoplast NN work_infoczas7nfbzac5mhjnxpkxle 253 9 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 253 10 fibroblast fibroblast NN work_infoczas7nfbzac5mhjnxpkxle 253 11 pair pair NN work_infoczas7nfbzac5mhjnxpkxle 253 12 during during IN work_infoczas7nfbzac5mhjnxpkxle 253 13 a a DT work_infoczas7nfbzac5mhjnxpkxle 253 14 second second JJ work_infoczas7nfbzac5mhjnxpkxle 253 15 fusion fusion NN work_infoczas7nfbzac5mhjnxpkxle 253 16 round round NN work_infoczas7nfbzac5mhjnxpkxle 253 17 which which WDT work_infoczas7nfbzac5mhjnxpkxle 253 18 also also RB work_infoczas7nfbzac5mhjnxpkxle 253 19 activated activate VBD work_infoczas7nfbzac5mhjnxpkxle 253 20 the the DT work_infoczas7nfbzac5mhjnxpkxle 253 21 reconstructed reconstruct VBN work_infoczas7nfbzac5mhjnxpkxle 253 22 embryo embryo NN work_infoczas7nfbzac5mhjnxpkxle 253 23 . . . work_infoczas7nfbzac5mhjnxpkxle 254 1 After after IN work_infoczas7nfbzac5mhjnxpkxle 254 2 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 254 3 days day NNS work_infoczas7nfbzac5mhjnxpkxle 254 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 254 5 in in FW work_infoczas7nfbzac5mhjnxpkxle 254 6 vitro vitro FW work_infoczas7nfbzac5mhjnxpkxle 254 7 cultured cultured NNP work_infoczas7nfbzac5mhjnxpkxle 254 8 embryos embryo NNS work_infoczas7nfbzac5mhjnxpkxle 254 9 were be VBD work_infoczas7nfbzac5mhjnxpkxle 254 10 developed develop VBN work_infoczas7nfbzac5mhjnxpkxle 254 11 into into IN work_infoczas7nfbzac5mhjnxpkxle 254 12 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 254 13 blastocysts blastocyst NNS work_infoczas7nfbzac5mhjnxpkxle 254 14 . . . work_infoczas7nfbzac5mhjnxpkxle 255 1 Day day NN work_infoczas7nfbzac5mhjnxpkxle 255 2 5 5 CD work_infoczas7nfbzac5mhjnxpkxle 255 3 and and CC work_infoczas7nfbzac5mhjnxpkxle 255 4 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 255 5 blastocysts blastocyst NNS work_infoczas7nfbzac5mhjnxpkxle 255 6 with with IN work_infoczas7nfbzac5mhjnxpkxle 255 7 clearly clearly RB work_infoczas7nfbzac5mhjnxpkxle 255 8 visible visible JJ work_infoczas7nfbzac5mhjnxpkxle 255 9 inner inner JJ work_infoczas7nfbzac5mhjnxpkxle 255 10 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 255 11 mass mass NN work_infoczas7nfbzac5mhjnxpkxle 255 12 were be VBD work_infoczas7nfbzac5mhjnxpkxle 255 13 surgically surgically RB work_infoczas7nfbzac5mhjnxpkxle 255 14 transferred transfer VBN work_infoczas7nfbzac5mhjnxpkxle 255 15 to to IN work_infoczas7nfbzac5mhjnxpkxle 255 16 a a DT work_infoczas7nfbzac5mhjnxpkxle 255 17 Danish danish JJ work_infoczas7nfbzac5mhjnxpkxle 255 18 landrace landrace NN work_infoczas7nfbzac5mhjnxpkxle 255 19 sow sow NN work_infoczas7nfbzac5mhjnxpkxle 255 20 on on IN work_infoczas7nfbzac5mhjnxpkxle 255 21 day day NN work_infoczas7nfbzac5mhjnxpkxle 255 22 4 4 CD work_infoczas7nfbzac5mhjnxpkxle 255 23 or or CC work_infoczas7nfbzac5mhjnxpkxle 255 24 5 5 CD work_infoczas7nfbzac5mhjnxpkxle 255 25 after after IN work_infoczas7nfbzac5mhjnxpkxle 255 26 weaning wean VBG work_infoczas7nfbzac5mhjnxpkxle 255 27 . . . work_infoczas7nfbzac5mhjnxpkxle 256 1 Pregnancy pregnancy NN work_infoczas7nfbzac5mhjnxpkxle 256 2 in in IN work_infoczas7nfbzac5mhjnxpkxle 256 3 the the DT work_infoczas7nfbzac5mhjnxpkxle 256 4 surrogate surrogate JJ work_infoczas7nfbzac5mhjnxpkxle 256 5 sow sow NN work_infoczas7nfbzac5mhjnxpkxle 256 6 was be VBD work_infoczas7nfbzac5mhjnxpkxle 256 7 diagnosed diagnose VBN work_infoczas7nfbzac5mhjnxpkxle 256 8 by by IN work_infoczas7nfbzac5mhjnxpkxle 256 9 ultrasonography ultrasonography NNP work_infoczas7nfbzac5mhjnxpkxle 256 10 on on IN work_infoczas7nfbzac5mhjnxpkxle 256 11 day day NN work_infoczas7nfbzac5mhjnxpkxle 256 12 21 21 CD work_infoczas7nfbzac5mhjnxpkxle 256 13 and and CC work_infoczas7nfbzac5mhjnxpkxle 256 14 confirmed confirm VBD work_infoczas7nfbzac5mhjnxpkxle 256 15 during during IN work_infoczas7nfbzac5mhjnxpkxle 256 16 later later JJ work_infoczas7nfbzac5mhjnxpkxle 256 17 stages stage NNS work_infoczas7nfbzac5mhjnxpkxle 256 18 of of IN work_infoczas7nfbzac5mhjnxpkxle 256 19 the the DT work_infoczas7nfbzac5mhjnxpkxle 256 20 pregnancy pregnancy NN work_infoczas7nfbzac5mhjnxpkxle 256 21 . . . work_infoczas7nfbzac5mhjnxpkxle 257 1 Pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 257 2 were be VBD work_infoczas7nfbzac5mhjnxpkxle 257 3 delivered deliver VBN work_infoczas7nfbzac5mhjnxpkxle 257 4 by by IN work_infoczas7nfbzac5mhjnxpkxle 257 5 natural natural JJ work_infoczas7nfbzac5mhjnxpkxle 257 6 birth birth NN work_infoczas7nfbzac5mhjnxpkxle 257 7 on on IN work_infoczas7nfbzac5mhjnxpkxle 257 8 day day NN work_infoczas7nfbzac5mhjnxpkxle 257 9 114 114 CD work_infoczas7nfbzac5mhjnxpkxle 257 10 and and CC work_infoczas7nfbzac5mhjnxpkxle 257 11 raised raise VBN work_infoczas7nfbzac5mhjnxpkxle 257 12 by by IN work_infoczas7nfbzac5mhjnxpkxle 257 13 their -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 257 14 surrogate surrogate JJ work_infoczas7nfbzac5mhjnxpkxle 257 15 mother mother NN work_infoczas7nfbzac5mhjnxpkxle 257 16 . . . work_infoczas7nfbzac5mhjnxpkxle 258 1 Pregnancy Pregnancy NNP work_infoczas7nfbzac5mhjnxpkxle 258 2 was be VBD work_infoczas7nfbzac5mhjnxpkxle 258 3 established establish VBN work_infoczas7nfbzac5mhjnxpkxle 258 4 by by IN work_infoczas7nfbzac5mhjnxpkxle 258 5 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 258 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 258 7 102 102 CD work_infoczas7nfbzac5mhjnxpkxle 258 8 cHS4.Ubi cHS4.Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 258 9 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 258 10 GIN.cHS4-transgenic GIN.cHS4-transgenic NNP work_infoczas7nfbzac5mhjnxpkxle 258 11 blastocysts blastocyst NNS work_infoczas7nfbzac5mhjnxpkxle 258 12 to to IN work_infoczas7nfbzac5mhjnxpkxle 258 13 the the DT work_infoczas7nfbzac5mhjnxpkxle 258 14 surrogate surrogate JJ work_infoczas7nfbzac5mhjnxpkxle 258 15 sow sow NN work_infoczas7nfbzac5mhjnxpkxle 258 16 . . . work_infoczas7nfbzac5mhjnxpkxle 259 1 Among among IN work_infoczas7nfbzac5mhjnxpkxle 259 2 nine nine CD work_infoczas7nfbzac5mhjnxpkxle 259 3 delivered deliver VBN work_infoczas7nfbzac5mhjnxpkxle 259 4 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 259 5 , , , work_infoczas7nfbzac5mhjnxpkxle 259 6 one one CD work_infoczas7nfbzac5mhjnxpkxle 259 7 was be VBD work_infoczas7nfbzac5mhjnxpkxle 259 8 stillborn stillborn VBN work_infoczas7nfbzac5mhjnxpkxle 259 9 and and CC work_infoczas7nfbzac5mhjnxpkxle 259 10 five five CD work_infoczas7nfbzac5mhjnxpkxle 259 11 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 259 12 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 259 13 referred refer VBN work_infoczas7nfbzac5mhjnxpkxle 259 14 to to IN work_infoczas7nfbzac5mhjnxpkxle 259 15 as as IN work_infoczas7nfbzac5mhjnxpkxle 259 16 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 259 17 # # $ work_infoczas7nfbzac5mhjnxpkxle 259 18 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 259 19 - - SYM work_infoczas7nfbzac5mhjnxpkxle 259 20 9 9 CD work_infoczas7nfbzac5mhjnxpkxle 259 21 and and CC work_infoczas7nfbzac5mhjnxpkxle 259 22 # # $ work_infoczas7nfbzac5mhjnxpkxle 259 23 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 259 24 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 259 25 survived survive VBD work_infoczas7nfbzac5mhjnxpkxle 259 26 past past NN work_infoczas7nfbzac5mhjnxpkxle 259 27 weaning wean VBG work_infoczas7nfbzac5mhjnxpkxle 259 28 . . . work_infoczas7nfbzac5mhjnxpkxle 260 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 260 2 non non JJ work_infoczas7nfbzac5mhjnxpkxle 260 3 - - JJ work_infoczas7nfbzac5mhjnxpkxle 260 4 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 260 5 wildtype wildtype NN work_infoczas7nfbzac5mhjnxpkxle 260 6 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 260 7 included include VBD work_infoczas7nfbzac5mhjnxpkxle 260 8 as as IN work_infoczas7nfbzac5mhjnxpkxle 260 9 a a DT work_infoczas7nfbzac5mhjnxpkxle 260 10 negative negative JJ work_infoczas7nfbzac5mhjnxpkxle 260 11 control control NN work_infoczas7nfbzac5mhjnxpkxle 260 12 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 260 13 was be VBD work_infoczas7nfbzac5mhjnxpkxle 260 14 designated designate VBN work_infoczas7nfbzac5mhjnxpkxle 260 15 # # $ work_infoczas7nfbzac5mhjnxpkxle 260 16 78 78 CD work_infoczas7nfbzac5mhjnxpkxle 260 17 . . . work_infoczas7nfbzac5mhjnxpkxle 261 1 Analysis analysis NN work_infoczas7nfbzac5mhjnxpkxle 261 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 261 3 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 261 4 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 261 5 5-mm 5-mm CD work_infoczas7nfbzac5mhjnxpkxle 261 6 ear ear NN work_infoczas7nfbzac5mhjnxpkxle 261 7 cuts cut NNS work_infoczas7nfbzac5mhjnxpkxle 261 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 261 9 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 261 10 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 261 11 from from IN work_infoczas7nfbzac5mhjnxpkxle 261 12 ear ear NN work_infoczas7nfbzac5mhjnxpkxle 261 13 biopsies biopsy NNS work_infoczas7nfbzac5mhjnxpkxle 261 14 were be VBD work_infoczas7nfbzac5mhjnxpkxle 261 15 analysed analyse VBN work_infoczas7nfbzac5mhjnxpkxle 261 16 by by IN work_infoczas7nfbzac5mhjnxpkxle 261 17 fluorescense fluorescense NN work_infoczas7nfbzac5mhjnxpkxle 261 18 microscopy microscopy JJ work_infoczas7nfbzac5mhjnxpkxle 261 19 . . . work_infoczas7nfbzac5mhjnxpkxle 262 1 Green green JJ work_infoczas7nfbzac5mhjnxpkxle 262 2 fluorescent fluorescent NN work_infoczas7nfbzac5mhjnxpkxle 262 3 protein protein NN work_infoczas7nfbzac5mhjnxpkxle 262 4 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 262 5 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 262 6 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 262 7 was be VBD work_infoczas7nfbzac5mhjnxpkxle 262 8 monitored monitor VBN work_infoczas7nfbzac5mhjnxpkxle 262 9 with with IN work_infoczas7nfbzac5mhjnxpkxle 262 10 a a DT work_infoczas7nfbzac5mhjnxpkxle 262 11 492 492 CD work_infoczas7nfbzac5mhjnxpkxle 262 12 nm nm IN work_infoczas7nfbzac5mhjnxpkxle 262 13 excitation excitation NN work_infoczas7nfbzac5mhjnxpkxle 262 14 filter filter NN work_infoczas7nfbzac5mhjnxpkxle 262 15 and and CC work_infoczas7nfbzac5mhjnxpkxle 262 16 a a DT work_infoczas7nfbzac5mhjnxpkxle 262 17 508 508 CD work_infoczas7nfbzac5mhjnxpkxle 262 18 nm nm CD work_infoczas7nfbzac5mhjnxpkxle 262 19 emission emission NN work_infoczas7nfbzac5mhjnxpkxle 262 20 filter filter NN work_infoczas7nfbzac5mhjnxpkxle 262 21 , , , work_infoczas7nfbzac5mhjnxpkxle 262 22 exposing expose VBG work_infoczas7nfbzac5mhjnxpkxle 262 23 for for IN work_infoczas7nfbzac5mhjnxpkxle 262 24 0.5 0.5 CD work_infoczas7nfbzac5mhjnxpkxle 262 25 sec sec NNP work_infoczas7nfbzac5mhjnxpkxle 262 26 . . . work_infoczas7nfbzac5mhjnxpkxle 263 1 Organs organ NNS work_infoczas7nfbzac5mhjnxpkxle 263 2 and and CC work_infoczas7nfbzac5mhjnxpkxle 263 3 tissues tissue NNS work_infoczas7nfbzac5mhjnxpkxle 263 4 from from IN work_infoczas7nfbzac5mhjnxpkxle 263 5 selected select VBN work_infoczas7nfbzac5mhjnxpkxle 263 6 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 263 7 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 263 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 263 9 control control NN work_infoczas7nfbzac5mhjnxpkxle 263 10 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 263 11 were be VBD work_infoczas7nfbzac5mhjnxpkxle 263 12 imaged image VBN work_infoczas7nfbzac5mhjnxpkxle 263 13 using use VBG work_infoczas7nfbzac5mhjnxpkxle 263 14 a a DT work_infoczas7nfbzac5mhjnxpkxle 263 15 Kodak Kodak NNP work_infoczas7nfbzac5mhjnxpkxle 263 16 Multispectral Multispectral NNP work_infoczas7nfbzac5mhjnxpkxle 263 17 In In NNP work_infoczas7nfbzac5mhjnxpkxle 263 18 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 263 19 Vivo Vivo NNP work_infoczas7nfbzac5mhjnxpkxle 263 20 FX FX NNP work_infoczas7nfbzac5mhjnxpkxle 263 21 machine machine NN work_infoczas7nfbzac5mhjnxpkxle 263 22 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 263 23 Carestream Carestream NNP work_infoczas7nfbzac5mhjnxpkxle 263 24 Inc Inc NNP work_infoczas7nfbzac5mhjnxpkxle 263 25 , , , work_infoczas7nfbzac5mhjnxpkxle 263 26 New New NNP work_infoczas7nfbzac5mhjnxpkxle 263 27 Haven Haven NNP work_infoczas7nfbzac5mhjnxpkxle 263 28 , , , work_infoczas7nfbzac5mhjnxpkxle 263 29 CT CT NNP work_infoczas7nfbzac5mhjnxpkxle 263 30 , , , work_infoczas7nfbzac5mhjnxpkxle 263 31 USA USA NNP work_infoczas7nfbzac5mhjnxpkxle 263 32 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 263 33 . . . work_infoczas7nfbzac5mhjnxpkxle 264 1 For for IN work_infoczas7nfbzac5mhjnxpkxle 264 2 each each DT work_infoczas7nfbzac5mhjnxpkxle 264 3 16 16 CD work_infoczas7nfbzac5mhjnxpkxle 264 4 organ organ NN work_infoczas7nfbzac5mhjnxpkxle 264 5 or or CC work_infoczas7nfbzac5mhjnxpkxle 264 6 tissue tissue NN work_infoczas7nfbzac5mhjnxpkxle 264 7 two two CD work_infoczas7nfbzac5mhjnxpkxle 264 8 images image NNS work_infoczas7nfbzac5mhjnxpkxle 264 9 were be VBD work_infoczas7nfbzac5mhjnxpkxle 264 10 acquired acquire VBN work_infoczas7nfbzac5mhjnxpkxle 264 11 , , , work_infoczas7nfbzac5mhjnxpkxle 264 12 one one CD work_infoczas7nfbzac5mhjnxpkxle 264 13 at at IN work_infoczas7nfbzac5mhjnxpkxle 264 14 white white JJ work_infoczas7nfbzac5mhjnxpkxle 264 15 light light NN work_infoczas7nfbzac5mhjnxpkxle 264 16 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 264 17 excitation excitation NN work_infoczas7nfbzac5mhjnxpkxle 264 18 : : : work_infoczas7nfbzac5mhjnxpkxle 264 19 0 0 CD work_infoczas7nfbzac5mhjnxpkxle 264 20 nm nm UH work_infoczas7nfbzac5mhjnxpkxle 264 21 ; ; : work_infoczas7nfbzac5mhjnxpkxle 264 22 emission emission NN work_infoczas7nfbzac5mhjnxpkxle 264 23 : : : work_infoczas7nfbzac5mhjnxpkxle 264 24 600 600 CD work_infoczas7nfbzac5mhjnxpkxle 264 25 nm nm CD work_infoczas7nfbzac5mhjnxpkxle 264 26 ; ; : work_infoczas7nfbzac5mhjnxpkxle 264 27 exposure exposure NN work_infoczas7nfbzac5mhjnxpkxle 264 28 time time NN work_infoczas7nfbzac5mhjnxpkxle 264 29 : : : work_infoczas7nfbzac5mhjnxpkxle 264 30 0.175 0.175 CD work_infoczas7nfbzac5mhjnxpkxle 264 31 sec sec NNP work_infoczas7nfbzac5mhjnxpkxle 264 32 ; ; : work_infoczas7nfbzac5mhjnxpkxle 264 33 F F NNP work_infoczas7nfbzac5mhjnxpkxle 264 34 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 264 35 stop:22.14 stop:22.14 NNP work_infoczas7nfbzac5mhjnxpkxle 264 36 ; ; : work_infoczas7nfbzac5mhjnxpkxle 264 37 no no DT work_infoczas7nfbzac5mhjnxpkxle 264 38 binning binning NN work_infoczas7nfbzac5mhjnxpkxle 264 39 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 264 40 and and CC work_infoczas7nfbzac5mhjnxpkxle 264 41 one one CD work_infoczas7nfbzac5mhjnxpkxle 264 42 by by IN work_infoczas7nfbzac5mhjnxpkxle 264 43 which which WDT work_infoczas7nfbzac5mhjnxpkxle 264 44 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 264 45 fluorescence fluorescence NN work_infoczas7nfbzac5mhjnxpkxle 264 46 was be VBD work_infoczas7nfbzac5mhjnxpkxle 264 47 monitored monitor VBN work_infoczas7nfbzac5mhjnxpkxle 264 48 by by IN work_infoczas7nfbzac5mhjnxpkxle 264 49 using use VBG work_infoczas7nfbzac5mhjnxpkxle 264 50 the the DT work_infoczas7nfbzac5mhjnxpkxle 264 51 multi multi JJ work_infoczas7nfbzac5mhjnxpkxle 264 52 - - JJ work_infoczas7nfbzac5mhjnxpkxle 264 53 wavelength wavelength JJ work_infoczas7nfbzac5mhjnxpkxle 264 54 function function NN work_infoczas7nfbzac5mhjnxpkxle 264 55 with with IN work_infoczas7nfbzac5mhjnxpkxle 264 56 a a DT work_infoczas7nfbzac5mhjnxpkxle 264 57 480 480 CD work_infoczas7nfbzac5mhjnxpkxle 264 58 nm nm NN work_infoczas7nfbzac5mhjnxpkxle 264 59 excitation excitation NN work_infoczas7nfbzac5mhjnxpkxle 264 60 filter filter NN work_infoczas7nfbzac5mhjnxpkxle 264 61 and and CC work_infoczas7nfbzac5mhjnxpkxle 264 62 a a DT work_infoczas7nfbzac5mhjnxpkxle 264 63 535 535 CD work_infoczas7nfbzac5mhjnxpkxle 264 64 nm nm CD work_infoczas7nfbzac5mhjnxpkxle 264 65 emission emission NN work_infoczas7nfbzac5mhjnxpkxle 264 66 filter filter NN work_infoczas7nfbzac5mhjnxpkxle 264 67 , , , work_infoczas7nfbzac5mhjnxpkxle 264 68 exposing expose VBG work_infoczas7nfbzac5mhjnxpkxle 264 69 for for IN work_infoczas7nfbzac5mhjnxpkxle 264 70 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 264 71 sec sec NNP work_infoczas7nfbzac5mhjnxpkxle 264 72 . . . work_infoczas7nfbzac5mhjnxpkxle 265 1 with with IN work_infoczas7nfbzac5mhjnxpkxle 265 2 f f NNP work_infoczas7nfbzac5mhjnxpkxle 265 3 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 265 4 stop stop VB work_infoczas7nfbzac5mhjnxpkxle 265 5 : : : work_infoczas7nfbzac5mhjnxpkxle 265 6 2.51 2.51 CD work_infoczas7nfbzac5mhjnxpkxle 265 7 and and CC work_infoczas7nfbzac5mhjnxpkxle 265 8 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 265 9 x x SYM work_infoczas7nfbzac5mhjnxpkxle 265 10 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 265 11 binning binning NN work_infoczas7nfbzac5mhjnxpkxle 265 12 . . . work_infoczas7nfbzac5mhjnxpkxle 266 1 Every every DT work_infoczas7nfbzac5mhjnxpkxle 266 2 two two CD work_infoczas7nfbzac5mhjnxpkxle 266 3 images image NNS work_infoczas7nfbzac5mhjnxpkxle 266 4 were be VBD work_infoczas7nfbzac5mhjnxpkxle 266 5 acquired acquire VBN work_infoczas7nfbzac5mhjnxpkxle 266 6 using use VBG work_infoczas7nfbzac5mhjnxpkxle 266 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 266 8 same same JJ work_infoczas7nfbzac5mhjnxpkxle 266 9 field field NN work_infoczas7nfbzac5mhjnxpkxle 266 10 of of IN work_infoczas7nfbzac5mhjnxpkxle 266 11 view view NN work_infoczas7nfbzac5mhjnxpkxle 266 12 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 266 13 FOV FOV NNP work_infoczas7nfbzac5mhjnxpkxle 266 14 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 266 15 . . . work_infoczas7nfbzac5mhjnxpkxle 267 1 After after IN work_infoczas7nfbzac5mhjnxpkxle 267 2 acquisition acquisition NN work_infoczas7nfbzac5mhjnxpkxle 267 3 , , , work_infoczas7nfbzac5mhjnxpkxle 267 4 pictures picture NNS work_infoczas7nfbzac5mhjnxpkxle 267 5 taken take VBN work_infoczas7nfbzac5mhjnxpkxle 267 6 in in IN work_infoczas7nfbzac5mhjnxpkxle 267 7 full full JJ work_infoczas7nfbzac5mhjnxpkxle 267 8 light light NN work_infoczas7nfbzac5mhjnxpkxle 267 9 were be VBD work_infoczas7nfbzac5mhjnxpkxle 267 10 added add VBN work_infoczas7nfbzac5mhjnxpkxle 267 11 a a DT work_infoczas7nfbzac5mhjnxpkxle 267 12 scale scale NN work_infoczas7nfbzac5mhjnxpkxle 267 13 bar bar NN work_infoczas7nfbzac5mhjnxpkxle 267 14 . . . work_infoczas7nfbzac5mhjnxpkxle 268 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 268 2 fluorescence fluorescence NN work_infoczas7nfbzac5mhjnxpkxle 268 3 emission emission NN work_infoczas7nfbzac5mhjnxpkxle 268 4 picture picture NN work_infoczas7nfbzac5mhjnxpkxle 268 5 was be VBD work_infoczas7nfbzac5mhjnxpkxle 268 6 artificially artificially RB work_infoczas7nfbzac5mhjnxpkxle 268 7 coloured colour VBN work_infoczas7nfbzac5mhjnxpkxle 268 8 green green NN work_infoczas7nfbzac5mhjnxpkxle 268 9 in in IN work_infoczas7nfbzac5mhjnxpkxle 268 10 the the DT work_infoczas7nfbzac5mhjnxpkxle 268 11 Kodak Kodak NNP work_infoczas7nfbzac5mhjnxpkxle 268 12 IM IM NNP work_infoczas7nfbzac5mhjnxpkxle 268 13 Software Software NNP work_infoczas7nfbzac5mhjnxpkxle 268 14 v1.0 v1.0 VBP work_infoczas7nfbzac5mhjnxpkxle 268 15 according accord VBG work_infoczas7nfbzac5mhjnxpkxle 268 16 to to IN work_infoczas7nfbzac5mhjnxpkxle 268 17 the the DT work_infoczas7nfbzac5mhjnxpkxle 268 18 signal signal NN work_infoczas7nfbzac5mhjnxpkxle 268 19 detected detect VBD work_infoczas7nfbzac5mhjnxpkxle 268 20 using use VBG work_infoczas7nfbzac5mhjnxpkxle 268 21 the the DT work_infoczas7nfbzac5mhjnxpkxle 268 22 specified specify VBN work_infoczas7nfbzac5mhjnxpkxle 268 23 filter filter NN work_infoczas7nfbzac5mhjnxpkxle 268 24 set set NN work_infoczas7nfbzac5mhjnxpkxle 268 25 . . . work_infoczas7nfbzac5mhjnxpkxle 269 1 Copy copy NN work_infoczas7nfbzac5mhjnxpkxle 269 2 number number NN work_infoczas7nfbzac5mhjnxpkxle 269 3 analysis analysis NN work_infoczas7nfbzac5mhjnxpkxle 269 4 by by IN work_infoczas7nfbzac5mhjnxpkxle 269 5 Southern Southern NNP work_infoczas7nfbzac5mhjnxpkxle 269 6 blotting blotting NN work_infoczas7nfbzac5mhjnxpkxle 269 7 Six six CD work_infoczas7nfbzac5mhjnxpkxle 269 8 µg µg IN work_infoczas7nfbzac5mhjnxpkxle 269 9 of of IN work_infoczas7nfbzac5mhjnxpkxle 269 10 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 269 11 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 269 12 was be VBD work_infoczas7nfbzac5mhjnxpkxle 269 13 digested digest VBN work_infoczas7nfbzac5mhjnxpkxle 269 14 with with IN work_infoczas7nfbzac5mhjnxpkxle 269 15 restriction restriction NN work_infoczas7nfbzac5mhjnxpkxle 269 16 enzymes enzyme NNS work_infoczas7nfbzac5mhjnxpkxle 269 17 , , , work_infoczas7nfbzac5mhjnxpkxle 269 18 loaded load VBN work_infoczas7nfbzac5mhjnxpkxle 269 19 on on IN work_infoczas7nfbzac5mhjnxpkxle 269 20 a a DT work_infoczas7nfbzac5mhjnxpkxle 269 21 0.7 0.7 CD work_infoczas7nfbzac5mhjnxpkxle 269 22 % % NN work_infoczas7nfbzac5mhjnxpkxle 269 23 agarose agarose JJ work_infoczas7nfbzac5mhjnxpkxle 269 24 gel gel NN work_infoczas7nfbzac5mhjnxpkxle 269 25 and and CC work_infoczas7nfbzac5mhjnxpkxle 269 26 electrophoresed electrophorese VBN work_infoczas7nfbzac5mhjnxpkxle 269 27 overnight overnight RB work_infoczas7nfbzac5mhjnxpkxle 269 28 at at IN work_infoczas7nfbzac5mhjnxpkxle 269 29 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 269 30 V v NN work_infoczas7nfbzac5mhjnxpkxle 269 31 / / SYM work_infoczas7nfbzac5mhjnxpkxle 269 32 cm cm NNS work_infoczas7nfbzac5mhjnxpkxle 269 33 . . . work_infoczas7nfbzac5mhjnxpkxle 270 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 270 2 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 270 3 was be VBD work_infoczas7nfbzac5mhjnxpkxle 270 4 transferred transfer VBN work_infoczas7nfbzac5mhjnxpkxle 270 5 to to IN work_infoczas7nfbzac5mhjnxpkxle 270 6 a a DT work_infoczas7nfbzac5mhjnxpkxle 270 7 nylon nylon NN work_infoczas7nfbzac5mhjnxpkxle 270 8 membrane membrane NN work_infoczas7nfbzac5mhjnxpkxle 270 9 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 270 10 Roche Roche NNP work_infoczas7nfbzac5mhjnxpkxle 270 11 , , , work_infoczas7nfbzac5mhjnxpkxle 270 12 Basel Basel NNP work_infoczas7nfbzac5mhjnxpkxle 270 13 , , , work_infoczas7nfbzac5mhjnxpkxle 270 14 Switzerland Switzerland NNP work_infoczas7nfbzac5mhjnxpkxle 270 15 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 270 16 by by IN work_infoczas7nfbzac5mhjnxpkxle 270 17 vacuum vacuum NN work_infoczas7nfbzac5mhjnxpkxle 270 18 suction suction NN work_infoczas7nfbzac5mhjnxpkxle 270 19 . . . work_infoczas7nfbzac5mhjnxpkxle 271 1 During during IN work_infoczas7nfbzac5mhjnxpkxle 271 2 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 271 3 , , , work_infoczas7nfbzac5mhjnxpkxle 271 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 271 5 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 271 6 was be VBD work_infoczas7nfbzac5mhjnxpkxle 271 7 nicked nicked JJ work_infoczas7nfbzac5mhjnxpkxle 271 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 271 9 denatured denature VBN work_infoczas7nfbzac5mhjnxpkxle 271 10 by by IN work_infoczas7nfbzac5mhjnxpkxle 271 11 0.25 0.25 CD work_infoczas7nfbzac5mhjnxpkxle 271 12 M M NNP work_infoczas7nfbzac5mhjnxpkxle 271 13 HCl HCl NNS work_infoczas7nfbzac5mhjnxpkxle 271 14 and and CC work_infoczas7nfbzac5mhjnxpkxle 271 15 1.5 1.5 CD work_infoczas7nfbzac5mhjnxpkxle 271 16 M M NNP work_infoczas7nfbzac5mhjnxpkxle 271 17 NaCl NaCl NNP work_infoczas7nfbzac5mhjnxpkxle 271 18 plus plus CC work_infoczas7nfbzac5mhjnxpkxle 271 19 0.5 0.5 CD work_infoczas7nfbzac5mhjnxpkxle 271 20 M m NN work_infoczas7nfbzac5mhjnxpkxle 271 21 NaOH naoh NN work_infoczas7nfbzac5mhjnxpkxle 271 22 , , , work_infoczas7nfbzac5mhjnxpkxle 271 23 respectively respectively RB work_infoczas7nfbzac5mhjnxpkxle 271 24 . . . work_infoczas7nfbzac5mhjnxpkxle 272 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 272 2 membrane membrane NN work_infoczas7nfbzac5mhjnxpkxle 272 3 was be VBD work_infoczas7nfbzac5mhjnxpkxle 272 4 prehybridized prehybridize VBN work_infoczas7nfbzac5mhjnxpkxle 272 5 in in IN work_infoczas7nfbzac5mhjnxpkxle 272 6 5xSSPE 5xsspe JJ work_infoczas7nfbzac5mhjnxpkxle 272 7 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 272 8 0.75 0.75 CD work_infoczas7nfbzac5mhjnxpkxle 272 9 M M NNP work_infoczas7nfbzac5mhjnxpkxle 272 10 NaCl NaCl NNP work_infoczas7nfbzac5mhjnxpkxle 272 11 , , , work_infoczas7nfbzac5mhjnxpkxle 272 12 0.05 0.05 CD work_infoczas7nfbzac5mhjnxpkxle 272 13 M m NN work_infoczas7nfbzac5mhjnxpkxle 272 14 NaH2PO4 NaH2PO4 NNP work_infoczas7nfbzac5mhjnxpkxle 272 15 , , , work_infoczas7nfbzac5mhjnxpkxle 272 16 0.005 0.005 CD work_infoczas7nfbzac5mhjnxpkxle 272 17 M M NNP work_infoczas7nfbzac5mhjnxpkxle 272 18 EDTA EDTA NNP work_infoczas7nfbzac5mhjnxpkxle 272 19 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 272 20 , , , work_infoczas7nfbzac5mhjnxpkxle 272 21 5x 5x NNP work_infoczas7nfbzac5mhjnxpkxle 272 22 Denhart Denhart NNP work_infoczas7nfbzac5mhjnxpkxle 272 23 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 272 24 0.1 0.1 CD work_infoczas7nfbzac5mhjnxpkxle 272 25 % % NN work_infoczas7nfbzac5mhjnxpkxle 272 26 Ficoll400 Ficoll400 NNP work_infoczas7nfbzac5mhjnxpkxle 272 27 , , , work_infoczas7nfbzac5mhjnxpkxle 272 28 0.1 0.1 CD work_infoczas7nfbzac5mhjnxpkxle 272 29 % % NN work_infoczas7nfbzac5mhjnxpkxle 272 30 polyvinylpyrrolidone polyvinylpyrrolidone NN work_infoczas7nfbzac5mhjnxpkxle 272 31 , , , work_infoczas7nfbzac5mhjnxpkxle 272 32 0.1 0.1 CD work_infoczas7nfbzac5mhjnxpkxle 272 33 % % NN work_infoczas7nfbzac5mhjnxpkxle 272 34 BSA BSA NNP work_infoczas7nfbzac5mhjnxpkxle 272 35 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 272 36 , , , work_infoczas7nfbzac5mhjnxpkxle 272 37 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 272 38 % % NN work_infoczas7nfbzac5mhjnxpkxle 272 39 SDS sds NN work_infoczas7nfbzac5mhjnxpkxle 272 40 , , , work_infoczas7nfbzac5mhjnxpkxle 272 41 50 50 CD work_infoczas7nfbzac5mhjnxpkxle 272 42 % % NN work_infoczas7nfbzac5mhjnxpkxle 272 43 formamide formamide NN work_infoczas7nfbzac5mhjnxpkxle 272 44 , , , work_infoczas7nfbzac5mhjnxpkxle 272 45 5 5 CD work_infoczas7nfbzac5mhjnxpkxle 272 46 % % NN work_infoczas7nfbzac5mhjnxpkxle 272 47 dextransulphate dextransulphate NN work_infoczas7nfbzac5mhjnxpkxle 272 48 and and CC work_infoczas7nfbzac5mhjnxpkxle 272 49 single single JJ work_infoczas7nfbzac5mhjnxpkxle 272 50 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 272 51 stranded strand VBN work_infoczas7nfbzac5mhjnxpkxle 272 52 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 272 53 carrier carrier NN work_infoczas7nfbzac5mhjnxpkxle 272 54 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 272 55 0.5 0.5 CD work_infoczas7nfbzac5mhjnxpkxle 272 56 mg mg NNP work_infoczas7nfbzac5mhjnxpkxle 272 57 / / SYM work_infoczas7nfbzac5mhjnxpkxle 272 58 ml ml NNP work_infoczas7nfbzac5mhjnxpkxle 272 59 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 272 60 overnight overnight RB work_infoczas7nfbzac5mhjnxpkxle 272 61 at at IN work_infoczas7nfbzac5mhjnxpkxle 272 62 42 42 CD work_infoczas7nfbzac5mhjnxpkxle 272 63 o o NN work_infoczas7nfbzac5mhjnxpkxle 272 64 C. C. NNP work_infoczas7nfbzac5mhjnxpkxle 272 65 The the DT work_infoczas7nfbzac5mhjnxpkxle 272 66 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 272 67 probe probe NN work_infoczas7nfbzac5mhjnxpkxle 272 68 , , , work_infoczas7nfbzac5mhjnxpkxle 272 69 a a DT work_infoczas7nfbzac5mhjnxpkxle 272 70 670-bp 670-bp CD work_infoczas7nfbzac5mhjnxpkxle 272 71 fragment fragment NN work_infoczas7nfbzac5mhjnxpkxle 272 72 of of IN work_infoczas7nfbzac5mhjnxpkxle 272 73 the the DT work_infoczas7nfbzac5mhjnxpkxle 272 74 neomycin neomycin NNP work_infoczas7nfbzac5mhjnxpkxle 272 75 resistance resistance NNP work_infoczas7nfbzac5mhjnxpkxle 272 76 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 272 77 , , , work_infoczas7nfbzac5mhjnxpkxle 272 78 was be VBD work_infoczas7nfbzac5mhjnxpkxle 272 79 labelled label VBN work_infoczas7nfbzac5mhjnxpkxle 272 80 with with IN work_infoczas7nfbzac5mhjnxpkxle 272 81 P P NNP work_infoczas7nfbzac5mhjnxpkxle 272 82 32 32 CD work_infoczas7nfbzac5mhjnxpkxle 272 83 -labelled -labelle VBN work_infoczas7nfbzac5mhjnxpkxle 272 84 CTP ctp NN work_infoczas7nfbzac5mhjnxpkxle 272 85 by by IN work_infoczas7nfbzac5mhjnxpkxle 272 86 random random JJ work_infoczas7nfbzac5mhjnxpkxle 272 87 priming priming NN work_infoczas7nfbzac5mhjnxpkxle 272 88 , , , work_infoczas7nfbzac5mhjnxpkxle 272 89 heat heat NN work_infoczas7nfbzac5mhjnxpkxle 272 90 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 272 91 denatured denature VBN work_infoczas7nfbzac5mhjnxpkxle 272 92 , , , work_infoczas7nfbzac5mhjnxpkxle 272 93 added add VBN work_infoczas7nfbzac5mhjnxpkxle 272 94 to to IN work_infoczas7nfbzac5mhjnxpkxle 272 95 prehybridization prehybridization NN work_infoczas7nfbzac5mhjnxpkxle 272 96 solution solution NN work_infoczas7nfbzac5mhjnxpkxle 272 97 , , , work_infoczas7nfbzac5mhjnxpkxle 272 98 and and CC work_infoczas7nfbzac5mhjnxpkxle 272 99 incubated incubate VBD work_infoczas7nfbzac5mhjnxpkxle 272 100 with with IN work_infoczas7nfbzac5mhjnxpkxle 272 101 the the DT work_infoczas7nfbzac5mhjnxpkxle 272 102 membrane membrane NN work_infoczas7nfbzac5mhjnxpkxle 272 103 overnight overnight RB work_infoczas7nfbzac5mhjnxpkxle 272 104 at at IN work_infoczas7nfbzac5mhjnxpkxle 272 105 42 42 CD work_infoczas7nfbzac5mhjnxpkxle 272 106 o o NN work_infoczas7nfbzac5mhjnxpkxle 272 107 C. C. NNP work_infoczas7nfbzac5mhjnxpkxle 272 108 The the DT work_infoczas7nfbzac5mhjnxpkxle 272 109 membrane membrane NN work_infoczas7nfbzac5mhjnxpkxle 272 110 was be VBD work_infoczas7nfbzac5mhjnxpkxle 272 111 washed wash VBN work_infoczas7nfbzac5mhjnxpkxle 272 112 in in IN work_infoczas7nfbzac5mhjnxpkxle 272 113 2x 2x CD work_infoczas7nfbzac5mhjnxpkxle 272 114 SSPE SSPE NNP work_infoczas7nfbzac5mhjnxpkxle 272 115 for for IN work_infoczas7nfbzac5mhjnxpkxle 272 116 2x5 2x5 CD work_infoczas7nfbzac5mhjnxpkxle 272 117 min min NN work_infoczas7nfbzac5mhjnxpkxle 272 118 at at IN work_infoczas7nfbzac5mhjnxpkxle 272 119 26 26 CD work_infoczas7nfbzac5mhjnxpkxle 272 120 o o NN work_infoczas7nfbzac5mhjnxpkxle 272 121 C C NNP work_infoczas7nfbzac5mhjnxpkxle 272 122 , , , work_infoczas7nfbzac5mhjnxpkxle 272 123 in in IN work_infoczas7nfbzac5mhjnxpkxle 272 124 2x 2x JJ work_infoczas7nfbzac5mhjnxpkxle 272 125 SSPE SSPE NNP work_infoczas7nfbzac5mhjnxpkxle 272 126 , , , work_infoczas7nfbzac5mhjnxpkxle 272 127 0.5 0.5 CD work_infoczas7nfbzac5mhjnxpkxle 272 128 % % NN work_infoczas7nfbzac5mhjnxpkxle 272 129 SDS sds NN work_infoczas7nfbzac5mhjnxpkxle 272 130 for for IN work_infoczas7nfbzac5mhjnxpkxle 272 131 2x15 2x15 CD work_infoczas7nfbzac5mhjnxpkxle 272 132 min min NN work_infoczas7nfbzac5mhjnxpkxle 272 133 at at IN work_infoczas7nfbzac5mhjnxpkxle 272 134 63 63 CD work_infoczas7nfbzac5mhjnxpkxle 272 135 o o NN work_infoczas7nfbzac5mhjnxpkxle 272 136 C c NN work_infoczas7nfbzac5mhjnxpkxle 272 137 , , , work_infoczas7nfbzac5mhjnxpkxle 272 138 and and CC work_infoczas7nfbzac5mhjnxpkxle 272 139 in in IN work_infoczas7nfbzac5mhjnxpkxle 272 140 0.2x 0.2x JJ work_infoczas7nfbzac5mhjnxpkxle 272 141 SSPE sspe JJ work_infoczas7nfbzac5mhjnxpkxle 272 142 , , , work_infoczas7nfbzac5mhjnxpkxle 272 143 0.5 0.5 CD work_infoczas7nfbzac5mhjnxpkxle 272 144 % % NN work_infoczas7nfbzac5mhjnxpkxle 272 145 SDS SDS NNP work_infoczas7nfbzac5mhjnxpkxle 272 146 for for IN work_infoczas7nfbzac5mhjnxpkxle 272 147 15 15 CD work_infoczas7nfbzac5mhjnxpkxle 272 148 min min NN work_infoczas7nfbzac5mhjnxpkxle 272 149 at at IN work_infoczas7nfbzac5mhjnxpkxle 272 150 63 63 CD work_infoczas7nfbzac5mhjnxpkxle 272 151 o o NN work_infoczas7nfbzac5mhjnxpkxle 272 152 C. C. NNP work_infoczas7nfbzac5mhjnxpkxle 272 153 The the DT work_infoczas7nfbzac5mhjnxpkxle 272 154 membrane membrane NN work_infoczas7nfbzac5mhjnxpkxle 272 155 was be VBD work_infoczas7nfbzac5mhjnxpkxle 272 156 air air NN work_infoczas7nfbzac5mhjnxpkxle 272 157 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 272 158 dried dry VBN work_infoczas7nfbzac5mhjnxpkxle 272 159 prior prior RB work_infoczas7nfbzac5mhjnxpkxle 272 160 to to IN work_infoczas7nfbzac5mhjnxpkxle 272 161 X x NN work_infoczas7nfbzac5mhjnxpkxle 272 162 - - NN work_infoczas7nfbzac5mhjnxpkxle 272 163 ray ray NN work_infoczas7nfbzac5mhjnxpkxle 272 164 film film NN work_infoczas7nfbzac5mhjnxpkxle 272 165 exposure exposure NN work_infoczas7nfbzac5mhjnxpkxle 272 166 . . . work_infoczas7nfbzac5mhjnxpkxle 273 1 Detection detection NN work_infoczas7nfbzac5mhjnxpkxle 273 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 273 3 genomically genomically RB work_infoczas7nfbzac5mhjnxpkxle 273 4 integrated integrate VBN work_infoczas7nfbzac5mhjnxpkxle 273 5 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 273 6 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 273 7 by by IN work_infoczas7nfbzac5mhjnxpkxle 273 8 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 273 9 To to TO work_infoczas7nfbzac5mhjnxpkxle 273 10 investigate investigate VB work_infoczas7nfbzac5mhjnxpkxle 273 11 whether whether IN work_infoczas7nfbzac5mhjnxpkxle 273 12 or or CC work_infoczas7nfbzac5mhjnxpkxle 273 13 not not RB work_infoczas7nfbzac5mhjnxpkxle 273 14 the the DT work_infoczas7nfbzac5mhjnxpkxle 273 15 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 273 16 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 273 17 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 273 18 encoding encode VBG work_infoczas7nfbzac5mhjnxpkxle 273 19 helper helper NN work_infoczas7nfbzac5mhjnxpkxle 273 20 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 273 21 had have VBD work_infoczas7nfbzac5mhjnxpkxle 273 22 been be VBN work_infoczas7nfbzac5mhjnxpkxle 273 23 incorporated incorporate VBN work_infoczas7nfbzac5mhjnxpkxle 273 24 into into IN work_infoczas7nfbzac5mhjnxpkxle 273 25 the the DT work_infoczas7nfbzac5mhjnxpkxle 273 26 genome genome NN work_infoczas7nfbzac5mhjnxpkxle 273 27 of of IN work_infoczas7nfbzac5mhjnxpkxle 273 28 the the DT work_infoczas7nfbzac5mhjnxpkxle 273 29 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 273 30 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 273 31 transgenic transgenic NNP work_infoczas7nfbzac5mhjnxpkxle 273 32 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 273 33 , , , work_infoczas7nfbzac5mhjnxpkxle 273 34 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 273 35 used use VBD work_infoczas7nfbzac5mhjnxpkxle 273 36 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 273 37 with with IN work_infoczas7nfbzac5mhjnxpkxle 273 38 two two CD work_infoczas7nfbzac5mhjnxpkxle 273 39 set set NN work_infoczas7nfbzac5mhjnxpkxle 273 40 of of IN work_infoczas7nfbzac5mhjnxpkxle 273 41 primers primer NNS work_infoczas7nfbzac5mhjnxpkxle 273 42 . . . work_infoczas7nfbzac5mhjnxpkxle 274 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 274 2 first first JJ work_infoczas7nfbzac5mhjnxpkxle 274 3 primer primer JJ work_infoczas7nfbzac5mhjnxpkxle 274 4 pair pair NN work_infoczas7nfbzac5mhjnxpkxle 274 5 17 17 CD work_infoczas7nfbzac5mhjnxpkxle 274 6 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 274 7 5´-GCTTAGGGTTAGGCGTTTTG-3 5´-gcttagggttaggcgttttg-3 CD work_infoczas7nfbzac5mhjnxpkxle 274 8 ´ ´ CD work_infoczas7nfbzac5mhjnxpkxle 274 9 and and CC work_infoczas7nfbzac5mhjnxpkxle 274 10 5´-GGTTATGTCGATATAGGACTCG-3 5´-ggttatgtcgatataggactcg-3 $ work_infoczas7nfbzac5mhjnxpkxle 274 11 ´ ´ CD work_infoczas7nfbzac5mhjnxpkxle 274 12 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 274 13 spanned span VBD work_infoczas7nfbzac5mhjnxpkxle 274 14 part part NN work_infoczas7nfbzac5mhjnxpkxle 274 15 of of IN work_infoczas7nfbzac5mhjnxpkxle 274 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 274 17 CMV CMV NNP work_infoczas7nfbzac5mhjnxpkxle 274 18 promoter promoter NN work_infoczas7nfbzac5mhjnxpkxle 274 19 and and CC work_infoczas7nfbzac5mhjnxpkxle 274 20 the the DT work_infoczas7nfbzac5mhjnxpkxle 274 21 5 5 CD work_infoczas7nfbzac5mhjnxpkxle 274 22 ´ ´ CD work_infoczas7nfbzac5mhjnxpkxle 274 23 ORF ORF NNP work_infoczas7nfbzac5mhjnxpkxle 274 24 in in IN work_infoczas7nfbzac5mhjnxpkxle 274 25 the the DT work_infoczas7nfbzac5mhjnxpkxle 274 26 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 274 27 . . . work_infoczas7nfbzac5mhjnxpkxle 275 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 275 2 second second JJ work_infoczas7nfbzac5mhjnxpkxle 275 3 primer primer JJ work_infoczas7nfbzac5mhjnxpkxle 275 4 pair pair NN work_infoczas7nfbzac5mhjnxpkxle 275 5 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 275 6 5´-GCCAGACTACGGTTTGCAAC-3 5´-gccagactacggtttgcaac-3 CD work_infoczas7nfbzac5mhjnxpkxle 275 7 ´ ´ CD work_infoczas7nfbzac5mhjnxpkxle 275 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 275 9 5´-CACCTTCCAGGGTCAAGGAAG-3 5´-caccttccagggtcaaggaag-3 CD work_infoczas7nfbzac5mhjnxpkxle 275 10 ´ ´ CD work_infoczas7nfbzac5mhjnxpkxle 275 11 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 275 12 amplified amplify VBD work_infoczas7nfbzac5mhjnxpkxle 275 13 the the DT work_infoczas7nfbzac5mhjnxpkxle 275 14 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 275 15 ORF ORF NNP work_infoczas7nfbzac5mhjnxpkxle 275 16 . . . work_infoczas7nfbzac5mhjnxpkxle 276 1 Identification identification NN work_infoczas7nfbzac5mhjnxpkxle 276 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 276 3 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 276 4 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 276 5 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 276 6 sites site NNS work_infoczas7nfbzac5mhjnxpkxle 276 7 by by IN work_infoczas7nfbzac5mhjnxpkxle 276 8 long long JJ work_infoczas7nfbzac5mhjnxpkxle 276 9 distance distance NN work_infoczas7nfbzac5mhjnxpkxle 276 10 inverse inverse NN work_infoczas7nfbzac5mhjnxpkxle 276 11 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 276 12 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 276 13 LDI LDI NNP work_infoczas7nfbzac5mhjnxpkxle 276 14 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 276 15 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 276 16 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 276 17 Templates template NNS work_infoczas7nfbzac5mhjnxpkxle 276 18 for for IN work_infoczas7nfbzac5mhjnxpkxle 276 19 amplification amplification NN work_infoczas7nfbzac5mhjnxpkxle 276 20 of of IN work_infoczas7nfbzac5mhjnxpkxle 276 21 junction junction NN work_infoczas7nfbzac5mhjnxpkxle 276 22 sites site NNS work_infoczas7nfbzac5mhjnxpkxle 276 23 between between IN work_infoczas7nfbzac5mhjnxpkxle 276 24 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 276 25 and and CC work_infoczas7nfbzac5mhjnxpkxle 276 26 genomic genomic NNP work_infoczas7nfbzac5mhjnxpkxle 276 27 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 276 28 were be VBD work_infoczas7nfbzac5mhjnxpkxle 276 29 generated generate VBN work_infoczas7nfbzac5mhjnxpkxle 276 30 as as IN work_infoczas7nfbzac5mhjnxpkxle 276 31 follows follow VBZ work_infoczas7nfbzac5mhjnxpkxle 276 32 : : : work_infoczas7nfbzac5mhjnxpkxle 276 33 0.5 0.5 CD work_infoczas7nfbzac5mhjnxpkxle 276 34 µg µg IN work_infoczas7nfbzac5mhjnxpkxle 276 35 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 276 36 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 276 37 was be VBD work_infoczas7nfbzac5mhjnxpkxle 276 38 digested digest VBN work_infoczas7nfbzac5mhjnxpkxle 276 39 with with IN work_infoczas7nfbzac5mhjnxpkxle 276 40 either either DT work_infoczas7nfbzac5mhjnxpkxle 276 41 BsrGI bsrgi NN work_infoczas7nfbzac5mhjnxpkxle 276 42 alone alone RB work_infoczas7nfbzac5mhjnxpkxle 276 43 or or CC work_infoczas7nfbzac5mhjnxpkxle 276 44 together together RB work_infoczas7nfbzac5mhjnxpkxle 276 45 with with IN work_infoczas7nfbzac5mhjnxpkxle 276 46 NdeI NdeI NNP work_infoczas7nfbzac5mhjnxpkxle 276 47 , , , work_infoczas7nfbzac5mhjnxpkxle 276 48 purified purify VBN work_infoczas7nfbzac5mhjnxpkxle 276 49 on on IN work_infoczas7nfbzac5mhjnxpkxle 276 50 a a DT work_infoczas7nfbzac5mhjnxpkxle 276 51 GFX GFX NNP work_infoczas7nfbzac5mhjnxpkxle 276 52 column column NN work_infoczas7nfbzac5mhjnxpkxle 276 53 and and CC work_infoczas7nfbzac5mhjnxpkxle 276 54 eluted elute VBD work_infoczas7nfbzac5mhjnxpkxle 276 55 in in IN work_infoczas7nfbzac5mhjnxpkxle 276 56 40 40 CD work_infoczas7nfbzac5mhjnxpkxle 276 57 µl µl IN work_infoczas7nfbzac5mhjnxpkxle 276 58 ddH2O. ddH2O. NNP work_infoczas7nfbzac5mhjnxpkxle 277 1 After after IN work_infoczas7nfbzac5mhjnxpkxle 277 2 adjustment adjustment NN work_infoczas7nfbzac5mhjnxpkxle 277 3 with with IN work_infoczas7nfbzac5mhjnxpkxle 277 4 ddH2O ddh2o NN work_infoczas7nfbzac5mhjnxpkxle 277 5 and and CC work_infoczas7nfbzac5mhjnxpkxle 277 6 10x 10x NNS work_infoczas7nfbzac5mhjnxpkxle 277 7 T4 T4 NNP work_infoczas7nfbzac5mhjnxpkxle 277 8 ligase ligase NN work_infoczas7nfbzac5mhjnxpkxle 277 9 buffer buffer VB work_infoczas7nfbzac5mhjnxpkxle 277 10 , , , work_infoczas7nfbzac5mhjnxpkxle 277 11 T4 T4 NNP work_infoczas7nfbzac5mhjnxpkxle 277 12 ligase ligase NN work_infoczas7nfbzac5mhjnxpkxle 277 13 was be VBD work_infoczas7nfbzac5mhjnxpkxle 277 14 added add VBN work_infoczas7nfbzac5mhjnxpkxle 277 15 , , , work_infoczas7nfbzac5mhjnxpkxle 277 16 and and CC work_infoczas7nfbzac5mhjnxpkxle 277 17 the the DT work_infoczas7nfbzac5mhjnxpkxle 277 18 500 500 CD work_infoczas7nfbzac5mhjnxpkxle 277 19 µl µl NNP work_infoczas7nfbzac5mhjnxpkxle 277 20 ligation ligation NN work_infoczas7nfbzac5mhjnxpkxle 277 21 mix mix NN work_infoczas7nfbzac5mhjnxpkxle 277 22 was be VBD work_infoczas7nfbzac5mhjnxpkxle 277 23 incubated incubate VBN work_infoczas7nfbzac5mhjnxpkxle 277 24 at at IN work_infoczas7nfbzac5mhjnxpkxle 277 25 16 16 CD work_infoczas7nfbzac5mhjnxpkxle 277 26 o o NN work_infoczas7nfbzac5mhjnxpkxle 277 27 C c NN work_infoczas7nfbzac5mhjnxpkxle 277 28 overnight overnight RB work_infoczas7nfbzac5mhjnxpkxle 277 29 . . . work_infoczas7nfbzac5mhjnxpkxle 278 1 Ligated Ligated NNP work_infoczas7nfbzac5mhjnxpkxle 278 2 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 278 3 was be VBD work_infoczas7nfbzac5mhjnxpkxle 278 4 purified purify VBN work_infoczas7nfbzac5mhjnxpkxle 278 5 on on IN work_infoczas7nfbzac5mhjnxpkxle 278 6 a a DT work_infoczas7nfbzac5mhjnxpkxle 278 7 GFX GFX NNP work_infoczas7nfbzac5mhjnxpkxle 278 8 column column NN work_infoczas7nfbzac5mhjnxpkxle 278 9 and and CC work_infoczas7nfbzac5mhjnxpkxle 278 10 eluded elude VBD work_infoczas7nfbzac5mhjnxpkxle 278 11 in in IN work_infoczas7nfbzac5mhjnxpkxle 278 12 40 40 CD work_infoczas7nfbzac5mhjnxpkxle 278 13 µl µl IN work_infoczas7nfbzac5mhjnxpkxle 278 14 ddH2O ddh2o NN work_infoczas7nfbzac5mhjnxpkxle 278 15 , , , work_infoczas7nfbzac5mhjnxpkxle 278 16 and and CC work_infoczas7nfbzac5mhjnxpkxle 278 17 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 278 18 µl µl CC work_infoczas7nfbzac5mhjnxpkxle 278 19 was be VBD work_infoczas7nfbzac5mhjnxpkxle 278 20 used use VBN work_infoczas7nfbzac5mhjnxpkxle 278 21 as as IN work_infoczas7nfbzac5mhjnxpkxle 278 22 template template NN work_infoczas7nfbzac5mhjnxpkxle 278 23 in in IN work_infoczas7nfbzac5mhjnxpkxle 278 24 the the DT work_infoczas7nfbzac5mhjnxpkxle 278 25 first first JJ work_infoczas7nfbzac5mhjnxpkxle 278 26 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 278 27 reaction reaction NN work_infoczas7nfbzac5mhjnxpkxle 278 28 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 278 29 33 33 CD work_infoczas7nfbzac5mhjnxpkxle 278 30 cycles cycle NNS work_infoczas7nfbzac5mhjnxpkxle 278 31 , , , work_infoczas7nfbzac5mhjnxpkxle 278 32 20 20 CD work_infoczas7nfbzac5mhjnxpkxle 278 33 pmol pmol NN work_infoczas7nfbzac5mhjnxpkxle 278 34 primers primer NNS work_infoczas7nfbzac5mhjnxpkxle 278 35 and and CC work_infoczas7nfbzac5mhjnxpkxle 278 36 annealing anneal VBG work_infoczas7nfbzac5mhjnxpkxle 278 37 temperature temperature NN work_infoczas7nfbzac5mhjnxpkxle 278 38 of of IN work_infoczas7nfbzac5mhjnxpkxle 278 39 59 59 CD work_infoczas7nfbzac5mhjnxpkxle 278 40 o o NN work_infoczas7nfbzac5mhjnxpkxle 278 41 C c NN work_infoczas7nfbzac5mhjnxpkxle 278 42 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 278 43 using use VBG work_infoczas7nfbzac5mhjnxpkxle 278 44 primers primer NNS work_infoczas7nfbzac5mhjnxpkxle 278 45 with with IN work_infoczas7nfbzac5mhjnxpkxle 278 46 recognition recognition NN work_infoczas7nfbzac5mhjnxpkxle 278 47 sequences sequence NNS work_infoczas7nfbzac5mhjnxpkxle 278 48 within within IN work_infoczas7nfbzac5mhjnxpkxle 278 49 the the DT work_infoczas7nfbzac5mhjnxpkxle 278 50 right right RB work_infoczas7nfbzac5mhjnxpkxle 278 51 inverted invert VBN work_infoczas7nfbzac5mhjnxpkxle 278 52 repeat repeat NN work_infoczas7nfbzac5mhjnxpkxle 278 53 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 278 54 5´-GTCCTGCTGGAGTTCGTGAC-3 5´-GTCCTGCTGGAGTTCGTGAC-3 NNP work_infoczas7nfbzac5mhjnxpkxle 278 55 ´ ´ CD work_infoczas7nfbzac5mhjnxpkxle 278 56 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 278 57 and and CC work_infoczas7nfbzac5mhjnxpkxle 278 58 within within IN work_infoczas7nfbzac5mhjnxpkxle 278 59 the the DT work_infoczas7nfbzac5mhjnxpkxle 278 60 neo neo NNP work_infoczas7nfbzac5mhjnxpkxle 278 61 r r NNP work_infoczas7nfbzac5mhjnxpkxle 278 62 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 278 63 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 278 64 5´- 5´- CD work_infoczas7nfbzac5mhjnxpkxle 278 65 GGCACAGTCAACTTAGTGTATG-3 GGCACAGTCAACTTAGTGTATG-3 NNP work_infoczas7nfbzac5mhjnxpkxle 278 66 ´ ´ NNP work_infoczas7nfbzac5mhjnxpkxle 278 67 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 278 68 . . . work_infoczas7nfbzac5mhjnxpkxle 279 1 Subsequently subsequently RB work_infoczas7nfbzac5mhjnxpkxle 279 2 , , , work_infoczas7nfbzac5mhjnxpkxle 279 3 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 279 4 µl µl IN work_infoczas7nfbzac5mhjnxpkxle 279 5 of of IN work_infoczas7nfbzac5mhjnxpkxle 279 6 this this DT work_infoczas7nfbzac5mhjnxpkxle 279 7 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 279 8 reaction reaction NN work_infoczas7nfbzac5mhjnxpkxle 279 9 was be VBD work_infoczas7nfbzac5mhjnxpkxle 279 10 used use VBN work_infoczas7nfbzac5mhjnxpkxle 279 11 as as IN work_infoczas7nfbzac5mhjnxpkxle 279 12 template template NN work_infoczas7nfbzac5mhjnxpkxle 279 13 in in IN work_infoczas7nfbzac5mhjnxpkxle 279 14 a a DT work_infoczas7nfbzac5mhjnxpkxle 279 15 nested nest VBN work_infoczas7nfbzac5mhjnxpkxle 279 16 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 279 17 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 279 18 30 30 CD work_infoczas7nfbzac5mhjnxpkxle 279 19 cycles cycle NNS work_infoczas7nfbzac5mhjnxpkxle 279 20 , , , work_infoczas7nfbzac5mhjnxpkxle 279 21 10 10 CD work_infoczas7nfbzac5mhjnxpkxle 279 22 pmol pmol NN work_infoczas7nfbzac5mhjnxpkxle 279 23 primers primer NNS work_infoczas7nfbzac5mhjnxpkxle 279 24 and and CC work_infoczas7nfbzac5mhjnxpkxle 279 25 annealing anneal VBG work_infoczas7nfbzac5mhjnxpkxle 279 26 temperature temperature NN work_infoczas7nfbzac5mhjnxpkxle 279 27 of of IN work_infoczas7nfbzac5mhjnxpkxle 279 28 58.5 58.5 CD work_infoczas7nfbzac5mhjnxpkxle 279 29 o o NN work_infoczas7nfbzac5mhjnxpkxle 279 30 C C NNP work_infoczas7nfbzac5mhjnxpkxle 279 31 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 279 32 using use VBG work_infoczas7nfbzac5mhjnxpkxle 279 33 the the DT work_infoczas7nfbzac5mhjnxpkxle 279 34 primers primer NNS work_infoczas7nfbzac5mhjnxpkxle 279 35 5´ATCACTCTCGGCATGGAC-3 5´atcactctcggcatggac-3 CD work_infoczas7nfbzac5mhjnxpkxle 279 36 ´ ´ CD work_infoczas7nfbzac5mhjnxpkxle 279 37 and and CC work_infoczas7nfbzac5mhjnxpkxle 279 38 5´-GATGTCCTAACTGACTTGCC-3 5´-gatgtcctaactgacttgcc-3 CD work_infoczas7nfbzac5mhjnxpkxle 279 39 ´ ´ CD work_infoczas7nfbzac5mhjnxpkxle 279 40 . . . work_infoczas7nfbzac5mhjnxpkxle 280 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 280 2 resulting result VBG work_infoczas7nfbzac5mhjnxpkxle 280 3 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 280 4 products product NNS work_infoczas7nfbzac5mhjnxpkxle 280 5 were be VBD work_infoczas7nfbzac5mhjnxpkxle 280 6 gel- gel- RB work_infoczas7nfbzac5mhjnxpkxle 280 7 purified purify VBN work_infoczas7nfbzac5mhjnxpkxle 280 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 280 9 sequenced sequence VBN work_infoczas7nfbzac5mhjnxpkxle 280 10 . . . work_infoczas7nfbzac5mhjnxpkxle 281 1 18 18 CD work_infoczas7nfbzac5mhjnxpkxle 281 2 ACKNOWLEDGMENTS acknowledgment NNS work_infoczas7nfbzac5mhjnxpkxle 281 3 We -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 281 4 thank thank VBP work_infoczas7nfbzac5mhjnxpkxle 281 5 Peter Peter NNP work_infoczas7nfbzac5mhjnxpkxle 281 6 Angels Angels NNP work_infoczas7nfbzac5mhjnxpkxle 281 7 for for IN work_infoczas7nfbzac5mhjnxpkxle 281 8 providing provide VBG work_infoczas7nfbzac5mhjnxpkxle 281 9 pUbiC pubic NN work_infoczas7nfbzac5mhjnxpkxle 281 10 - - : work_infoczas7nfbzac5mhjnxpkxle 281 11 Jun Jun NNP work_infoczas7nfbzac5mhjnxpkxle 281 12 . . . work_infoczas7nfbzac5mhjnxpkxle 282 1 We -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 282 2 thank thank VBP work_infoczas7nfbzac5mhjnxpkxle 282 3 Tina Tina NNP work_infoczas7nfbzac5mhjnxpkxle 282 4 Fuglsang Fuglsang NNP work_infoczas7nfbzac5mhjnxpkxle 282 5 and and CC work_infoczas7nfbzac5mhjnxpkxle 282 6 Marianne Marianne NNP work_infoczas7nfbzac5mhjnxpkxle 282 7 Gregers Gregers NNP work_infoczas7nfbzac5mhjnxpkxle 282 8 Johansen Johansen NNP work_infoczas7nfbzac5mhjnxpkxle 282 9 for for IN work_infoczas7nfbzac5mhjnxpkxle 282 10 excellent excellent JJ work_infoczas7nfbzac5mhjnxpkxle 282 11 technical technical JJ work_infoczas7nfbzac5mhjnxpkxle 282 12 assistance assistance NN work_infoczas7nfbzac5mhjnxpkxle 282 13 . . . work_infoczas7nfbzac5mhjnxpkxle 283 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 283 2 work work NN work_infoczas7nfbzac5mhjnxpkxle 283 3 by by IN work_infoczas7nfbzac5mhjnxpkxle 283 4 J.E.J. J.E.J. NNP work_infoczas7nfbzac5mhjnxpkxle 284 1 was be VBD work_infoczas7nfbzac5mhjnxpkxle 284 2 carried carry VBN work_infoczas7nfbzac5mhjnxpkxle 284 3 out out RP work_infoczas7nfbzac5mhjnxpkxle 284 4 in in IN work_infoczas7nfbzac5mhjnxpkxle 284 5 the the DT work_infoczas7nfbzac5mhjnxpkxle 284 6 laboratory laboratory NN work_infoczas7nfbzac5mhjnxpkxle 284 7 of of IN work_infoczas7nfbzac5mhjnxpkxle 284 8 A.L.J. A.L.J. NNP work_infoczas7nfbzac5mhjnxpkxle 285 1 This this DT work_infoczas7nfbzac5mhjnxpkxle 285 2 work work NN work_infoczas7nfbzac5mhjnxpkxle 285 3 was be VBD work_infoczas7nfbzac5mhjnxpkxle 285 4 made make VBN work_infoczas7nfbzac5mhjnxpkxle 285 5 possible possible JJ work_infoczas7nfbzac5mhjnxpkxle 285 6 through through IN work_infoczas7nfbzac5mhjnxpkxle 285 7 support support NN work_infoczas7nfbzac5mhjnxpkxle 285 8 from from IN work_infoczas7nfbzac5mhjnxpkxle 285 9 the the DT work_infoczas7nfbzac5mhjnxpkxle 285 10 Danish Danish NNP work_infoczas7nfbzac5mhjnxpkxle 285 11 National National NNP work_infoczas7nfbzac5mhjnxpkxle 285 12 Advanced Advanced NNP work_infoczas7nfbzac5mhjnxpkxle 285 13 Technology Technology NNP work_infoczas7nfbzac5mhjnxpkxle 285 14 Foundation Foundation NNP work_infoczas7nfbzac5mhjnxpkxle 285 15 , , , work_infoczas7nfbzac5mhjnxpkxle 285 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 285 17 Lundbeck Lundbeck NNP work_infoczas7nfbzac5mhjnxpkxle 285 18 Foundation Foundation NNP work_infoczas7nfbzac5mhjnxpkxle 285 19 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 285 20 grant grant VB work_infoczas7nfbzac5mhjnxpkxle 285 21 no no UH work_infoczas7nfbzac5mhjnxpkxle 285 22 . . . work_infoczas7nfbzac5mhjnxpkxle 286 1 R19-A2108 R19-A2108 NNP work_infoczas7nfbzac5mhjnxpkxle 286 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 286 3 , , , work_infoczas7nfbzac5mhjnxpkxle 286 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 286 5 Danish Danish NNP work_infoczas7nfbzac5mhjnxpkxle 286 6 Research Research NNP work_infoczas7nfbzac5mhjnxpkxle 286 7 Agency Agency NNP work_infoczas7nfbzac5mhjnxpkxle 286 8 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 286 9 grant grant VB work_infoczas7nfbzac5mhjnxpkxle 286 10 no no UH work_infoczas7nfbzac5mhjnxpkxle 286 11 . . . work_infoczas7nfbzac5mhjnxpkxle 287 1 274 274 CD work_infoczas7nfbzac5mhjnxpkxle 287 2 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 287 3 05 05 CD work_infoczas7nfbzac5mhjnxpkxle 287 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 287 5 0197 0197 CD work_infoczas7nfbzac5mhjnxpkxle 287 6 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 287 7 , , , work_infoczas7nfbzac5mhjnxpkxle 287 8 the the DT work_infoczas7nfbzac5mhjnxpkxle 287 9 Danish Danish NNP work_infoczas7nfbzac5mhjnxpkxle 287 10 Medical Medical NNP work_infoczas7nfbzac5mhjnxpkxle 287 11 Research Research NNP work_infoczas7nfbzac5mhjnxpkxle 287 12 Council Council NNP work_infoczas7nfbzac5mhjnxpkxle 287 13 , , , work_infoczas7nfbzac5mhjnxpkxle 287 14 the the DT work_infoczas7nfbzac5mhjnxpkxle 287 15 Novo Novo NNP work_infoczas7nfbzac5mhjnxpkxle 287 16 Nordisk Nordisk NNP work_infoczas7nfbzac5mhjnxpkxle 287 17 Foundation Foundation NNP work_infoczas7nfbzac5mhjnxpkxle 287 18 , , , work_infoczas7nfbzac5mhjnxpkxle 287 19 the the DT work_infoczas7nfbzac5mhjnxpkxle 287 20 Carlsberg Carlsberg NNP work_infoczas7nfbzac5mhjnxpkxle 287 21 Foundation Foundation NNP work_infoczas7nfbzac5mhjnxpkxle 287 22 , , , work_infoczas7nfbzac5mhjnxpkxle 287 23 the the DT work_infoczas7nfbzac5mhjnxpkxle 287 24 Danish Danish NNP work_infoczas7nfbzac5mhjnxpkxle 287 25 Cancer Cancer NNP work_infoczas7nfbzac5mhjnxpkxle 287 26 Society Society NNP work_infoczas7nfbzac5mhjnxpkxle 287 27 , , , work_infoczas7nfbzac5mhjnxpkxle 287 28 the the DT work_infoczas7nfbzac5mhjnxpkxle 287 29 Danish Danish NNP work_infoczas7nfbzac5mhjnxpkxle 287 30 Heart Heart NNP work_infoczas7nfbzac5mhjnxpkxle 287 31 Association Association NNP work_infoczas7nfbzac5mhjnxpkxle 287 32 , , , work_infoczas7nfbzac5mhjnxpkxle 287 33 Aage Aage NNP work_infoczas7nfbzac5mhjnxpkxle 287 34 Bangs Bangs NNP work_infoczas7nfbzac5mhjnxpkxle 287 35 Foundation Foundation NNP work_infoczas7nfbzac5mhjnxpkxle 287 36 , , , work_infoczas7nfbzac5mhjnxpkxle 287 37 the the DT work_infoczas7nfbzac5mhjnxpkxle 287 38 Augustinus Augustinus NNP work_infoczas7nfbzac5mhjnxpkxle 287 39 Foundation Foundation NNP work_infoczas7nfbzac5mhjnxpkxle 287 40 , , , work_infoczas7nfbzac5mhjnxpkxle 287 41 and and CC work_infoczas7nfbzac5mhjnxpkxle 287 42 the the DT work_infoczas7nfbzac5mhjnxpkxle 287 43 EU EU NNP work_infoczas7nfbzac5mhjnxpkxle 287 44 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 287 45 EU EU NNP work_infoczas7nfbzac5mhjnxpkxle 287 46 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 287 47 FP6-STREP FP6-STREP NNP work_infoczas7nfbzac5mhjnxpkxle 287 48 , , , work_infoczas7nfbzac5mhjnxpkxle 287 49 contract contract NN work_infoczas7nfbzac5mhjnxpkxle 287 50 number number NN work_infoczas7nfbzac5mhjnxpkxle 287 51 018961 018961 CD work_infoczas7nfbzac5mhjnxpkxle 287 52 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 287 53 . . . work_infoczas7nfbzac5mhjnxpkxle 288 1 B.M B.M NNP work_infoczas7nfbzac5mhjnxpkxle 288 2 was be VBD work_infoczas7nfbzac5mhjnxpkxle 288 3 funded fund VBN work_infoczas7nfbzac5mhjnxpkxle 288 4 by by IN work_infoczas7nfbzac5mhjnxpkxle 288 5 a a DT work_infoczas7nfbzac5mhjnxpkxle 288 6 grant grant NN work_infoczas7nfbzac5mhjnxpkxle 288 7 from from IN work_infoczas7nfbzac5mhjnxpkxle 288 8 the the DT work_infoczas7nfbzac5mhjnxpkxle 288 9 Danish Danish NNP work_infoczas7nfbzac5mhjnxpkxle 288 10 Cancer Cancer NNP work_infoczas7nfbzac5mhjnxpkxle 288 11 Society Society NNP work_infoczas7nfbzac5mhjnxpkxle 288 12 . . . work_infoczas7nfbzac5mhjnxpkxle 289 1 19 19 CD work_infoczas7nfbzac5mhjnxpkxle 289 2 FIGURE figure NN work_infoczas7nfbzac5mhjnxpkxle 289 3 LEGENDS legend NNS work_infoczas7nfbzac5mhjnxpkxle 289 4 Figure figure NN work_infoczas7nfbzac5mhjnxpkxle 289 5 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 289 6 . . . work_infoczas7nfbzac5mhjnxpkxle 290 1 Generation generation NN work_infoczas7nfbzac5mhjnxpkxle 290 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 290 3 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 290 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 290 5 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 290 6 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 290 7 by by IN work_infoczas7nfbzac5mhjnxpkxle 290 8 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 290 9 of of IN work_infoczas7nfbzac5mhjnxpkxle 290 10 insulated insulated JJ work_infoczas7nfbzac5mhjnxpkxle 290 11 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 290 12 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 290 13 encoding encode VBG work_infoczas7nfbzac5mhjnxpkxle 290 14 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 290 15 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 290 16 in in IN work_infoczas7nfbzac5mhjnxpkxle 290 17 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 290 18 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 290 19 . . . work_infoczas7nfbzac5mhjnxpkxle 291 1 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 291 2 A a DT work_infoczas7nfbzac5mhjnxpkxle 291 3 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 291 4 Schematic schematic JJ work_infoczas7nfbzac5mhjnxpkxle 291 5 map map NN work_infoczas7nfbzac5mhjnxpkxle 291 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 291 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 291 8 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 291 9 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 291 10 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 291 11 , , , work_infoczas7nfbzac5mhjnxpkxle 291 12 pSBT pSBT NNP work_infoczas7nfbzac5mhjnxpkxle 291 13 / / SYM work_infoczas7nfbzac5mhjnxpkxle 291 14 cHS4.Ubi cHS4.Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 291 15 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 291 16 GIN.cHS4 GIN.cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 291 17 , , , work_infoczas7nfbzac5mhjnxpkxle 291 18 used use VBD work_infoczas7nfbzac5mhjnxpkxle 291 19 to to TO work_infoczas7nfbzac5mhjnxpkxle 291 20 create create VB work_infoczas7nfbzac5mhjnxpkxle 291 21 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 291 22 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 291 23 . . . work_infoczas7nfbzac5mhjnxpkxle 292 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 292 2 borders border NNS work_infoczas7nfbzac5mhjnxpkxle 292 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 292 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 292 5 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 292 6 are be VBP work_infoczas7nfbzac5mhjnxpkxle 292 7 indicated indicate VBN work_infoczas7nfbzac5mhjnxpkxle 292 8 by by IN work_infoczas7nfbzac5mhjnxpkxle 292 9 grey grey NN work_infoczas7nfbzac5mhjnxpkxle 292 10 arrows arrow NNS work_infoczas7nfbzac5mhjnxpkxle 292 11 representing represent VBG work_infoczas7nfbzac5mhjnxpkxle 292 12 the the DT work_infoczas7nfbzac5mhjnxpkxle 292 13 left left NN work_infoczas7nfbzac5mhjnxpkxle 292 14 and and CC work_infoczas7nfbzac5mhjnxpkxle 292 15 right right JJ work_infoczas7nfbzac5mhjnxpkxle 292 16 inverted invert VBN work_infoczas7nfbzac5mhjnxpkxle 292 17 repeats repeat NNS work_infoczas7nfbzac5mhjnxpkxle 292 18 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 292 19 LIR LIR NNP work_infoczas7nfbzac5mhjnxpkxle 292 20 and and CC work_infoczas7nfbzac5mhjnxpkxle 292 21 RIR RIR NNP work_infoczas7nfbzac5mhjnxpkxle 292 22 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 292 23 . . . work_infoczas7nfbzac5mhjnxpkxle 293 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 293 2 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 293 3 contains contain VBZ work_infoczas7nfbzac5mhjnxpkxle 293 4 a a DT work_infoczas7nfbzac5mhjnxpkxle 293 5 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 293 6 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 293 7 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 293 8 IRES IRES NNP work_infoczas7nfbzac5mhjnxpkxle 293 9 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 293 10 neo neo NNP work_infoczas7nfbzac5mhjnxpkxle 293 11 r r NN work_infoczas7nfbzac5mhjnxpkxle 293 12 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 293 13 flanked flank VBN work_infoczas7nfbzac5mhjnxpkxle 293 14 by by IN work_infoczas7nfbzac5mhjnxpkxle 293 15 1.2-kb 1.2-kb CD work_infoczas7nfbzac5mhjnxpkxle 293 16 long long JJ work_infoczas7nfbzac5mhjnxpkxle 293 17 5’-HS4 5’-HS4 NNP work_infoczas7nfbzac5mhjnxpkxle 293 18 chicken chicken NN work_infoczas7nfbzac5mhjnxpkxle 293 19 β β NNP work_infoczas7nfbzac5mhjnxpkxle 293 20 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 293 21 globin globin NNP work_infoczas7nfbzac5mhjnxpkxle 293 22 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 293 23 cHS4 cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 293 24 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 293 25 insulators insulator NNS work_infoczas7nfbzac5mhjnxpkxle 293 26 . . . work_infoczas7nfbzac5mhjnxpkxle 294 1 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 294 2 B b NN work_infoczas7nfbzac5mhjnxpkxle 294 3 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 294 4 Stable stable JJ work_infoczas7nfbzac5mhjnxpkxle 294 5 transfection transfection NN work_infoczas7nfbzac5mhjnxpkxle 294 6 efficiency efficiency NN work_infoczas7nfbzac5mhjnxpkxle 294 7 in in IN work_infoczas7nfbzac5mhjnxpkxle 294 8 NPFs NPFs NNP work_infoczas7nfbzac5mhjnxpkxle 294 9 . . . work_infoczas7nfbzac5mhjnxpkxle 295 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 295 2 diagram diagram NN work_infoczas7nfbzac5mhjnxpkxle 295 3 depicts depict VBZ work_infoczas7nfbzac5mhjnxpkxle 295 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 295 5 number number NN work_infoczas7nfbzac5mhjnxpkxle 295 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 295 7 G418-resistant g418-resistant JJ work_infoczas7nfbzac5mhjnxpkxle 295 8 colonies colony NNS work_infoczas7nfbzac5mhjnxpkxle 295 9 measured measure VBN work_infoczas7nfbzac5mhjnxpkxle 295 10 by by IN work_infoczas7nfbzac5mhjnxpkxle 295 11 co co NN work_infoczas7nfbzac5mhjnxpkxle 295 12 - - NN work_infoczas7nfbzac5mhjnxpkxle 295 13 transfection transfection NN work_infoczas7nfbzac5mhjnxpkxle 295 14 of of IN work_infoczas7nfbzac5mhjnxpkxle 295 15 NPFs npf NNS work_infoczas7nfbzac5mhjnxpkxle 295 16 with with IN work_infoczas7nfbzac5mhjnxpkxle 295 17 pSBT psbt JJ work_infoczas7nfbzac5mhjnxpkxle 295 18 / / SYM work_infoczas7nfbzac5mhjnxpkxle 295 19 cHS4.Ubi cHS4.Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 295 20 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 295 21 GIN.cHS4 GIN.cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 295 22 with with IN work_infoczas7nfbzac5mhjnxpkxle 295 23 either either DT work_infoczas7nfbzac5mhjnxpkxle 295 24 pUC19 pUC19 NNS work_infoczas7nfbzac5mhjnxpkxle 295 25 or or CC work_infoczas7nfbzac5mhjnxpkxle 295 26 the the DT work_infoczas7nfbzac5mhjnxpkxle 295 27 HSB3-encoding hsb3-encode VBG work_infoczas7nfbzac5mhjnxpkxle 295 28 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 295 29 pCMV pCMV NNP work_infoczas7nfbzac5mhjnxpkxle 295 30 - - : work_infoczas7nfbzac5mhjnxpkxle 295 31 HSB3 HSB3 NNP work_infoczas7nfbzac5mhjnxpkxle 295 32 using use VBG work_infoczas7nfbzac5mhjnxpkxle 295 33 a a DT work_infoczas7nfbzac5mhjnxpkxle 295 34 40:1 40:1 CD work_infoczas7nfbzac5mhjnxpkxle 295 35 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 295 36 ratio ratio NN work_infoczas7nfbzac5mhjnxpkxle 295 37 . . . work_infoczas7nfbzac5mhjnxpkxle 296 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 296 2 experiment experiment NN work_infoczas7nfbzac5mhjnxpkxle 296 3 was be VBD work_infoczas7nfbzac5mhjnxpkxle 296 4 performed perform VBN work_infoczas7nfbzac5mhjnxpkxle 296 5 in in IN work_infoczas7nfbzac5mhjnxpkxle 296 6 triplicates triplicate NNS work_infoczas7nfbzac5mhjnxpkxle 296 7 and and CC work_infoczas7nfbzac5mhjnxpkxle 296 8 the the DT work_infoczas7nfbzac5mhjnxpkxle 296 9 data datum NNS work_infoczas7nfbzac5mhjnxpkxle 296 10 are be VBP work_infoczas7nfbzac5mhjnxpkxle 296 11 presented present VBN work_infoczas7nfbzac5mhjnxpkxle 296 12 as as IN work_infoczas7nfbzac5mhjnxpkxle 296 13 mean mean NNP work_infoczas7nfbzac5mhjnxpkxle 296 14 ± ± NNP work_infoczas7nfbzac5mhjnxpkxle 296 15 SD sd NN work_infoczas7nfbzac5mhjnxpkxle 296 16 . . . work_infoczas7nfbzac5mhjnxpkxle 297 1 A a DT work_infoczas7nfbzac5mhjnxpkxle 297 2 mean mean NN work_infoczas7nfbzac5mhjnxpkxle 297 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 297 4 7 7 CD work_infoczas7nfbzac5mhjnxpkxle 297 5 and and CC work_infoczas7nfbzac5mhjnxpkxle 297 6 90 90 CD work_infoczas7nfbzac5mhjnxpkxle 297 7 colonies colony NNS work_infoczas7nfbzac5mhjnxpkxle 297 8 was be VBD work_infoczas7nfbzac5mhjnxpkxle 297 9 observed observe VBN work_infoczas7nfbzac5mhjnxpkxle 297 10 in in IN work_infoczas7nfbzac5mhjnxpkxle 297 11 cultures culture NNS work_infoczas7nfbzac5mhjnxpkxle 297 12 transfected transfecte VBN work_infoczas7nfbzac5mhjnxpkxle 297 13 with with IN work_infoczas7nfbzac5mhjnxpkxle 297 14 pUC19 pUC19 NNS work_infoczas7nfbzac5mhjnxpkxle 297 15 and and CC work_infoczas7nfbzac5mhjnxpkxle 297 16 pCMV pCMV NNP work_infoczas7nfbzac5mhjnxpkxle 297 17 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 297 18 HSB3 HSB3 NNP work_infoczas7nfbzac5mhjnxpkxle 297 19 , , , work_infoczas7nfbzac5mhjnxpkxle 297 20 respectively respectively RB work_infoczas7nfbzac5mhjnxpkxle 297 21 . . . work_infoczas7nfbzac5mhjnxpkxle 298 1 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 298 2 C C NNP work_infoczas7nfbzac5mhjnxpkxle 298 3 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 298 4 Five five CD work_infoczas7nfbzac5mhjnxpkxle 298 5 young young JJ work_infoczas7nfbzac5mhjnxpkxle 298 6 Göttingen Göttingen NNP work_infoczas7nfbzac5mhjnxpkxle 298 7 minipigs minipig NNS work_infoczas7nfbzac5mhjnxpkxle 298 8 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 298 9 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 298 10 # # $ work_infoczas7nfbzac5mhjnxpkxle 298 11 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 298 12 - - SYM work_infoczas7nfbzac5mhjnxpkxle 298 13 9 9 CD work_infoczas7nfbzac5mhjnxpkxle 298 14 and and CC work_infoczas7nfbzac5mhjnxpkxle 298 15 # # $ work_infoczas7nfbzac5mhjnxpkxle 298 16 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 298 17 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 298 18 established establish VBN work_infoczas7nfbzac5mhjnxpkxle 298 19 by by IN work_infoczas7nfbzac5mhjnxpkxle 298 20 SCNT SCNT NNP work_infoczas7nfbzac5mhjnxpkxle 298 21 . . . work_infoczas7nfbzac5mhjnxpkxle 299 1 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 299 2 D d NN work_infoczas7nfbzac5mhjnxpkxle 299 3 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 299 4 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 299 5 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 299 6 in in IN work_infoczas7nfbzac5mhjnxpkxle 299 7 ear ear NN work_infoczas7nfbzac5mhjnxpkxle 299 8 cuts cut NNS work_infoczas7nfbzac5mhjnxpkxle 299 9 from from IN work_infoczas7nfbzac5mhjnxpkxle 299 10 the the DT work_infoczas7nfbzac5mhjnxpkxle 299 11 five five CD work_infoczas7nfbzac5mhjnxpkxle 299 12 Göttingen Göttingen NNP work_infoczas7nfbzac5mhjnxpkxle 299 13 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 299 14 . . . work_infoczas7nfbzac5mhjnxpkxle 300 1 Ear ear NN work_infoczas7nfbzac5mhjnxpkxle 300 2 cuts cut NNS work_infoczas7nfbzac5mhjnxpkxle 300 3 were be VBD work_infoczas7nfbzac5mhjnxpkxle 300 4 analyzed analyze VBN work_infoczas7nfbzac5mhjnxpkxle 300 5 in in IN work_infoczas7nfbzac5mhjnxpkxle 300 6 normal normal JJ work_infoczas7nfbzac5mhjnxpkxle 300 7 light light NN work_infoczas7nfbzac5mhjnxpkxle 300 8 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 300 9 a a DT work_infoczas7nfbzac5mhjnxpkxle 300 10 , , , work_infoczas7nfbzac5mhjnxpkxle 300 11 c c NN work_infoczas7nfbzac5mhjnxpkxle 300 12 , , , work_infoczas7nfbzac5mhjnxpkxle 300 13 e e NNP work_infoczas7nfbzac5mhjnxpkxle 300 14 , , , work_infoczas7nfbzac5mhjnxpkxle 300 15 g g NN work_infoczas7nfbzac5mhjnxpkxle 300 16 , , , work_infoczas7nfbzac5mhjnxpkxle 300 17 and and CC work_infoczas7nfbzac5mhjnxpkxle 300 18 i i PRP work_infoczas7nfbzac5mhjnxpkxle 300 19 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 300 20 and and CC work_infoczas7nfbzac5mhjnxpkxle 300 21 under under IN work_infoczas7nfbzac5mhjnxpkxle 300 22 blue blue JJ work_infoczas7nfbzac5mhjnxpkxle 300 23 light light NN work_infoczas7nfbzac5mhjnxpkxle 300 24 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 300 25 b b NN work_infoczas7nfbzac5mhjnxpkxle 300 26 , , , work_infoczas7nfbzac5mhjnxpkxle 300 27 d d NNP work_infoczas7nfbzac5mhjnxpkxle 300 28 , , , work_infoczas7nfbzac5mhjnxpkxle 300 29 f f NNP work_infoczas7nfbzac5mhjnxpkxle 300 30 , , , work_infoczas7nfbzac5mhjnxpkxle 300 31 h h NNP work_infoczas7nfbzac5mhjnxpkxle 300 32 , , , work_infoczas7nfbzac5mhjnxpkxle 300 33 and and CC work_infoczas7nfbzac5mhjnxpkxle 300 34 j j NNP work_infoczas7nfbzac5mhjnxpkxle 300 35 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 300 36 . . . work_infoczas7nfbzac5mhjnxpkxle 301 1 Samples sample NNS work_infoczas7nfbzac5mhjnxpkxle 301 2 were be VBD work_infoczas7nfbzac5mhjnxpkxle 301 3 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 301 4 from from IN work_infoczas7nfbzac5mhjnxpkxle 301 5 the the DT work_infoczas7nfbzac5mhjnxpkxle 301 6 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 301 7 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 301 8 # # $ work_infoczas7nfbzac5mhjnxpkxle 301 9 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 301 10 - - SYM work_infoczas7nfbzac5mhjnxpkxle 301 11 9 9 CD work_infoczas7nfbzac5mhjnxpkxle 301 12 and and CC work_infoczas7nfbzac5mhjnxpkxle 301 13 # # $ work_infoczas7nfbzac5mhjnxpkxle 301 14 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 301 15 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 301 16 indicated indicate VBD work_infoczas7nfbzac5mhjnxpkxle 301 17 above above IN work_infoczas7nfbzac5mhjnxpkxle 301 18 each each DT work_infoczas7nfbzac5mhjnxpkxle 301 19 set set VBN work_infoczas7nfbzac5mhjnxpkxle 301 20 up up RP work_infoczas7nfbzac5mhjnxpkxle 301 21 of of IN work_infoczas7nfbzac5mhjnxpkxle 301 22 pictures picture NNS work_infoczas7nfbzac5mhjnxpkxle 301 23 . . . work_infoczas7nfbzac5mhjnxpkxle 302 1 Figure figure NN work_infoczas7nfbzac5mhjnxpkxle 302 2 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 302 3 . . . work_infoczas7nfbzac5mhjnxpkxle 303 1 Genetic genetic JJ work_infoczas7nfbzac5mhjnxpkxle 303 2 analysis analysis NN work_infoczas7nfbzac5mhjnxpkxle 303 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 303 4 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 303 5 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 303 6 harboring harbor VBG work_infoczas7nfbzac5mhjnxpkxle 303 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 303 8 SBT SBT NNP work_infoczas7nfbzac5mhjnxpkxle 303 9 / / SYM work_infoczas7nfbzac5mhjnxpkxle 303 10 cHS4.Ubi cHS4.Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 303 11 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 303 12 GIN.cHS4 GIN.cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 303 13 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 303 14 . . . work_infoczas7nfbzac5mhjnxpkxle 304 1 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 304 2 A a DT work_infoczas7nfbzac5mhjnxpkxle 304 3 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 304 4 Schematic schematic JJ work_infoczas7nfbzac5mhjnxpkxle 304 5 map map NN work_infoczas7nfbzac5mhjnxpkxle 304 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 304 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 304 8 pSBT psbt NN work_infoczas7nfbzac5mhjnxpkxle 304 9 / / SYM work_infoczas7nfbzac5mhjnxpkxle 304 10 cHS4.Ubi cHS4.Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 304 11 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 304 12 GIN.cHS4 GIN.cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 304 13 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 304 14 with with IN work_infoczas7nfbzac5mhjnxpkxle 304 15 relevant relevant JJ work_infoczas7nfbzac5mhjnxpkxle 304 16 restriction restriction NN work_infoczas7nfbzac5mhjnxpkxle 304 17 sites site NNS work_infoczas7nfbzac5mhjnxpkxle 304 18 , , , work_infoczas7nfbzac5mhjnxpkxle 304 19 used use VBN work_infoczas7nfbzac5mhjnxpkxle 304 20 in in IN work_infoczas7nfbzac5mhjnxpkxle 304 21 the the DT work_infoczas7nfbzac5mhjnxpkxle 304 22 Southern southern JJ work_infoczas7nfbzac5mhjnxpkxle 304 23 blot blot NN work_infoczas7nfbzac5mhjnxpkxle 304 24 analysis analysis NN work_infoczas7nfbzac5mhjnxpkxle 304 25 , , , work_infoczas7nfbzac5mhjnxpkxle 304 26 indicated indicate VBN work_infoczas7nfbzac5mhjnxpkxle 304 27 by by IN work_infoczas7nfbzac5mhjnxpkxle 304 28 dashed dash VBN work_infoczas7nfbzac5mhjnxpkxle 304 29 lines line NNS work_infoczas7nfbzac5mhjnxpkxle 304 30 . . . work_infoczas7nfbzac5mhjnxpkxle 305 1 Restriction restriction NN work_infoczas7nfbzac5mhjnxpkxle 305 2 sites site NNS work_infoczas7nfbzac5mhjnxpkxle 305 3 used use VBN work_infoczas7nfbzac5mhjnxpkxle 305 4 for for IN work_infoczas7nfbzac5mhjnxpkxle 305 5 LDI LDI NNP work_infoczas7nfbzac5mhjnxpkxle 305 6 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 305 7 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 305 8 are be VBP work_infoczas7nfbzac5mhjnxpkxle 305 9 shown show VBN work_infoczas7nfbzac5mhjnxpkxle 305 10 in in IN work_infoczas7nfbzac5mhjnxpkxle 305 11 bold bold JJ work_infoczas7nfbzac5mhjnxpkxle 305 12 and and CC work_infoczas7nfbzac5mhjnxpkxle 305 13 marked mark VBN work_infoczas7nfbzac5mhjnxpkxle 305 14 with with IN work_infoczas7nfbzac5mhjnxpkxle 305 15 arrows arrow NNS work_infoczas7nfbzac5mhjnxpkxle 305 16 . . . work_infoczas7nfbzac5mhjnxpkxle 306 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 306 2 small small JJ work_infoczas7nfbzac5mhjnxpkxle 306 3 arrowheads arrowhead NNS work_infoczas7nfbzac5mhjnxpkxle 306 4 indicate indicate VBP work_infoczas7nfbzac5mhjnxpkxle 306 5 location location NN work_infoczas7nfbzac5mhjnxpkxle 306 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 306 7 primers primer NNS work_infoczas7nfbzac5mhjnxpkxle 306 8 used use VBN work_infoczas7nfbzac5mhjnxpkxle 306 9 in in IN work_infoczas7nfbzac5mhjnxpkxle 306 10 the the DT work_infoczas7nfbzac5mhjnxpkxle 306 11 LDI LDI NNP work_infoczas7nfbzac5mhjnxpkxle 306 12 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 306 13 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 306 14 . . . work_infoczas7nfbzac5mhjnxpkxle 307 1 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 307 2 B b NN work_infoczas7nfbzac5mhjnxpkxle 307 3 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 307 4 Southern southern JJ work_infoczas7nfbzac5mhjnxpkxle 307 5 blot blot NN work_infoczas7nfbzac5mhjnxpkxle 307 6 analysis analysis NN work_infoczas7nfbzac5mhjnxpkxle 307 7 of of IN work_infoczas7nfbzac5mhjnxpkxle 307 8 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 307 9 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 307 10 isolated isolate VBN work_infoczas7nfbzac5mhjnxpkxle 307 11 from from IN work_infoczas7nfbzac5mhjnxpkxle 307 12 SBT SBT NNP work_infoczas7nfbzac5mhjnxpkxle 307 13 / / SYM work_infoczas7nfbzac5mhjnxpkxle 307 14 cHS4.Ubi cHS4.Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 307 15 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 307 16 GIN.cHS4-transgenic gin.chs4-transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 307 17 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 307 18 and and CC work_infoczas7nfbzac5mhjnxpkxle 307 19 digested digest VBN work_infoczas7nfbzac5mhjnxpkxle 307 20 with with IN work_infoczas7nfbzac5mhjnxpkxle 307 21 XbaI. XbaI. NNP work_infoczas7nfbzac5mhjnxpkxle 308 1 A a DT work_infoczas7nfbzac5mhjnxpkxle 308 2 670-bp 670-bp CD work_infoczas7nfbzac5mhjnxpkxle 308 3 neo neo NN work_infoczas7nfbzac5mhjnxpkxle 308 4 r r NN work_infoczas7nfbzac5mhjnxpkxle 308 5 fragment fragment NN work_infoczas7nfbzac5mhjnxpkxle 308 6 was be VBD work_infoczas7nfbzac5mhjnxpkxle 308 7 used use VBN work_infoczas7nfbzac5mhjnxpkxle 308 8 as as IN work_infoczas7nfbzac5mhjnxpkxle 308 9 probe probe NN work_infoczas7nfbzac5mhjnxpkxle 308 10 . . . work_infoczas7nfbzac5mhjnxpkxle 309 1 Lanes Lanes NNP work_infoczas7nfbzac5mhjnxpkxle 309 2 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 309 3 - - SYM work_infoczas7nfbzac5mhjnxpkxle 309 4 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 309 5 contain contain NN work_infoczas7nfbzac5mhjnxpkxle 309 6 XbaI XbaI NNP work_infoczas7nfbzac5mhjnxpkxle 309 7 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 309 8 digested digest VBN work_infoczas7nfbzac5mhjnxpkxle 309 9 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 309 10 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 309 11 from from IN work_infoczas7nfbzac5mhjnxpkxle 309 12 the the DT work_infoczas7nfbzac5mhjnxpkxle 309 13 five five CD work_infoczas7nfbzac5mhjnxpkxle 309 14 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 309 15 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 309 16 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 309 17 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 309 18 shown show VBN work_infoczas7nfbzac5mhjnxpkxle 309 19 in in IN work_infoczas7nfbzac5mhjnxpkxle 309 20 Figure figure NN work_infoczas7nfbzac5mhjnxpkxle 309 21 1C. 1c. NN work_infoczas7nfbzac5mhjnxpkxle 310 1 Negative negative JJ work_infoczas7nfbzac5mhjnxpkxle 310 2 controls control NNS work_infoczas7nfbzac5mhjnxpkxle 310 3 are be VBP work_infoczas7nfbzac5mhjnxpkxle 310 4 provided provide VBN work_infoczas7nfbzac5mhjnxpkxle 310 5 in in IN work_infoczas7nfbzac5mhjnxpkxle 310 6 lanes lanes NNP work_infoczas7nfbzac5mhjnxpkxle 310 7 7 7 CD work_infoczas7nfbzac5mhjnxpkxle 310 8 and and CC work_infoczas7nfbzac5mhjnxpkxle 310 9 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 310 10 which which WDT work_infoczas7nfbzac5mhjnxpkxle 310 11 contain contain VBP work_infoczas7nfbzac5mhjnxpkxle 310 12 XbaI XbaI NNP work_infoczas7nfbzac5mhjnxpkxle 310 13 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 310 14 digested digest VBN work_infoczas7nfbzac5mhjnxpkxle 310 15 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 310 16 from from IN work_infoczas7nfbzac5mhjnxpkxle 310 17 a a DT work_infoczas7nfbzac5mhjnxpkxle 310 18 control control NN work_infoczas7nfbzac5mhjnxpkxle 310 19 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 310 20 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 310 21 # # $ work_infoczas7nfbzac5mhjnxpkxle 310 22 78 78 CD work_infoczas7nfbzac5mhjnxpkxle 310 23 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 310 24 and and CC work_infoczas7nfbzac5mhjnxpkxle 310 25 a a DT work_infoczas7nfbzac5mhjnxpkxle 310 26 porcine porcine JJ work_infoczas7nfbzac5mhjnxpkxle 310 27 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 310 28 line line NN work_infoczas7nfbzac5mhjnxpkxle 310 29 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 310 30 PFV PFV NNP work_infoczas7nfbzac5mhjnxpkxle 310 31 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 310 32 which which WDT work_infoczas7nfbzac5mhjnxpkxle 310 33 did do VBD work_infoczas7nfbzac5mhjnxpkxle 310 34 not not RB work_infoczas7nfbzac5mhjnxpkxle 310 35 20 20 CD work_infoczas7nfbzac5mhjnxpkxle 310 36 contain contain VB work_infoczas7nfbzac5mhjnxpkxle 310 37 the the DT work_infoczas7nfbzac5mhjnxpkxle 310 38 neo neo NNP work_infoczas7nfbzac5mhjnxpkxle 310 39 r r NN work_infoczas7nfbzac5mhjnxpkxle 310 40 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 310 41 , , , work_infoczas7nfbzac5mhjnxpkxle 310 42 respectively respectively RB work_infoczas7nfbzac5mhjnxpkxle 310 43 . . . work_infoczas7nfbzac5mhjnxpkxle 311 1 Pig pig NN work_infoczas7nfbzac5mhjnxpkxle 311 2 identification identification NN work_infoczas7nfbzac5mhjnxpkxle 311 3 numbers number NNS work_infoczas7nfbzac5mhjnxpkxle 311 4 are be VBP work_infoczas7nfbzac5mhjnxpkxle 311 5 provided provide VBN work_infoczas7nfbzac5mhjnxpkxle 311 6 above above IN work_infoczas7nfbzac5mhjnxpkxle 311 7 each each DT work_infoczas7nfbzac5mhjnxpkxle 311 8 lane lane NN work_infoczas7nfbzac5mhjnxpkxle 311 9 . . . work_infoczas7nfbzac5mhjnxpkxle 312 1 Lane Lane NNP work_infoczas7nfbzac5mhjnxpkxle 312 2 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 312 3 contains contain VBZ work_infoczas7nfbzac5mhjnxpkxle 312 4 a a DT work_infoczas7nfbzac5mhjnxpkxle 312 5 marker marker NN work_infoczas7nfbzac5mhjnxpkxle 312 6 . . . work_infoczas7nfbzac5mhjnxpkxle 313 1 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 313 2 C C NNP work_infoczas7nfbzac5mhjnxpkxle 313 3 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 313 4 The the DT work_infoczas7nfbzac5mhjnxpkxle 313 5 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 313 6 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 313 7 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 313 8 could could MD work_infoczas7nfbzac5mhjnxpkxle 313 9 not not RB work_infoczas7nfbzac5mhjnxpkxle 313 10 be be VB work_infoczas7nfbzac5mhjnxpkxle 313 11 detected detect VBN work_infoczas7nfbzac5mhjnxpkxle 313 12 in in IN work_infoczas7nfbzac5mhjnxpkxle 313 13 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 313 14 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 313 15 . . . work_infoczas7nfbzac5mhjnxpkxle 314 1 Lanes Lanes NNP work_infoczas7nfbzac5mhjnxpkxle 314 2 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 314 3 - - SYM work_infoczas7nfbzac5mhjnxpkxle 314 4 5 5 CD work_infoczas7nfbzac5mhjnxpkxle 314 5 and and CC work_infoczas7nfbzac5mhjnxpkxle 314 6 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 314 7 - - SYM work_infoczas7nfbzac5mhjnxpkxle 314 8 12 12 CD work_infoczas7nfbzac5mhjnxpkxle 314 9 represent represent NN work_infoczas7nfbzac5mhjnxpkxle 314 10 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 314 11 reactions reaction NNS work_infoczas7nfbzac5mhjnxpkxle 314 12 performed perform VBN work_infoczas7nfbzac5mhjnxpkxle 314 13 on on IN work_infoczas7nfbzac5mhjnxpkxle 314 14 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 314 15 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 314 16 from from IN work_infoczas7nfbzac5mhjnxpkxle 314 17 each each DT work_infoczas7nfbzac5mhjnxpkxle 314 18 of of IN work_infoczas7nfbzac5mhjnxpkxle 314 19 the the DT work_infoczas7nfbzac5mhjnxpkxle 314 20 five five CD work_infoczas7nfbzac5mhjnxpkxle 314 21 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 314 22 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 314 23 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 314 24 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 314 25 using use VBG work_infoczas7nfbzac5mhjnxpkxle 314 26 two two CD work_infoczas7nfbzac5mhjnxpkxle 314 27 primer primer JJ work_infoczas7nfbzac5mhjnxpkxle 314 28 sets set NNS work_infoczas7nfbzac5mhjnxpkxle 314 29 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 314 30 one one CD work_infoczas7nfbzac5mhjnxpkxle 314 31 amplifying amplify VBG work_infoczas7nfbzac5mhjnxpkxle 314 32 part part NN work_infoczas7nfbzac5mhjnxpkxle 314 33 of of IN work_infoczas7nfbzac5mhjnxpkxle 314 34 the the DT work_infoczas7nfbzac5mhjnxpkxle 314 35 CMV CMV NNP work_infoczas7nfbzac5mhjnxpkxle 314 36 promoter promoter NN work_infoczas7nfbzac5mhjnxpkxle 314 37 plus plus CC work_infoczas7nfbzac5mhjnxpkxle 314 38 upstream upstream JJ work_infoczas7nfbzac5mhjnxpkxle 314 39 part part NN work_infoczas7nfbzac5mhjnxpkxle 314 40 of of IN work_infoczas7nfbzac5mhjnxpkxle 314 41 the the DT work_infoczas7nfbzac5mhjnxpkxle 314 42 HSB3 HSB3 NNP work_infoczas7nfbzac5mhjnxpkxle 314 43 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 314 44 and and CC work_infoczas7nfbzac5mhjnxpkxle 314 45 one one CD work_infoczas7nfbzac5mhjnxpkxle 314 46 amplifying amplify VBG work_infoczas7nfbzac5mhjnxpkxle 314 47 the the DT work_infoczas7nfbzac5mhjnxpkxle 314 48 HSB3 HSB3 NNP work_infoczas7nfbzac5mhjnxpkxle 314 49 ORF ORF NNP work_infoczas7nfbzac5mhjnxpkxle 314 50 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 314 51 . . . work_infoczas7nfbzac5mhjnxpkxle 315 1 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 315 2 reactions reaction NNS work_infoczas7nfbzac5mhjnxpkxle 315 3 performed perform VBN work_infoczas7nfbzac5mhjnxpkxle 315 4 on on IN work_infoczas7nfbzac5mhjnxpkxle 315 5 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 315 6 from from IN work_infoczas7nfbzac5mhjnxpkxle 315 7 a a DT work_infoczas7nfbzac5mhjnxpkxle 315 8 wildtype wildtype NN work_infoczas7nfbzac5mhjnxpkxle 315 9 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 315 10 were be VBD work_infoczas7nfbzac5mhjnxpkxle 315 11 loaded load VBN work_infoczas7nfbzac5mhjnxpkxle 315 12 in in IN work_infoczas7nfbzac5mhjnxpkxle 315 13 lanes lanes NNP work_infoczas7nfbzac5mhjnxpkxle 315 14 6 6 CD work_infoczas7nfbzac5mhjnxpkxle 315 15 and and CC work_infoczas7nfbzac5mhjnxpkxle 315 16 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 315 17 , , , work_infoczas7nfbzac5mhjnxpkxle 315 18 whereas whereas IN work_infoczas7nfbzac5mhjnxpkxle 315 19 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 315 20 reactions reaction NNS work_infoczas7nfbzac5mhjnxpkxle 315 21 performed perform VBN work_infoczas7nfbzac5mhjnxpkxle 315 22 on on IN work_infoczas7nfbzac5mhjnxpkxle 315 23 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 315 24 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 315 25 from from IN work_infoczas7nfbzac5mhjnxpkxle 315 26 a a DT work_infoczas7nfbzac5mhjnxpkxle 315 27 control control NN work_infoczas7nfbzac5mhjnxpkxle 315 28 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 315 29 containing contain VBG work_infoczas7nfbzac5mhjnxpkxle 315 30 a a DT work_infoczas7nfbzac5mhjnxpkxle 315 31 genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 315 32 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 315 33 of of IN work_infoczas7nfbzac5mhjnxpkxle 315 34 the the DT work_infoczas7nfbzac5mhjnxpkxle 315 35 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 315 36 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 315 37 were be VBD work_infoczas7nfbzac5mhjnxpkxle 315 38 included include VBN work_infoczas7nfbzac5mhjnxpkxle 315 39 as as IN work_infoczas7nfbzac5mhjnxpkxle 315 40 positive positive JJ work_infoczas7nfbzac5mhjnxpkxle 315 41 controls control NNS work_infoczas7nfbzac5mhjnxpkxle 315 42 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 315 43 lanes lanes NNP work_infoczas7nfbzac5mhjnxpkxle 315 44 7 7 CD work_infoczas7nfbzac5mhjnxpkxle 315 45 and and CC work_infoczas7nfbzac5mhjnxpkxle 315 46 14 14 CD work_infoczas7nfbzac5mhjnxpkxle 315 47 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 315 48 . . . work_infoczas7nfbzac5mhjnxpkxle 316 1 Figure figure NN work_infoczas7nfbzac5mhjnxpkxle 316 2 3 3 CD work_infoczas7nfbzac5mhjnxpkxle 316 3 . . . work_infoczas7nfbzac5mhjnxpkxle 317 1 Identification identification NN work_infoczas7nfbzac5mhjnxpkxle 317 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 317 3 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 317 4 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 317 5 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 317 6 sites site NNS work_infoczas7nfbzac5mhjnxpkxle 317 7 in in IN work_infoczas7nfbzac5mhjnxpkxle 317 8 intergenic intergenic JJ work_infoczas7nfbzac5mhjnxpkxle 317 9 regions region NNS work_infoczas7nfbzac5mhjnxpkxle 317 10 of of IN work_infoczas7nfbzac5mhjnxpkxle 317 11 the the DT work_infoczas7nfbzac5mhjnxpkxle 317 12 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 317 13 genome genome NN work_infoczas7nfbzac5mhjnxpkxle 317 14 . . . work_infoczas7nfbzac5mhjnxpkxle 318 1 Junction junction NN work_infoczas7nfbzac5mhjnxpkxle 318 2 sites site NNS work_infoczas7nfbzac5mhjnxpkxle 318 3 between between IN work_infoczas7nfbzac5mhjnxpkxle 318 4 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 318 5 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 318 6 and and CC work_infoczas7nfbzac5mhjnxpkxle 318 7 flanking flank VBG work_infoczas7nfbzac5mhjnxpkxle 318 8 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 318 9 were be VBD work_infoczas7nfbzac5mhjnxpkxle 318 10 identified identify VBN work_infoczas7nfbzac5mhjnxpkxle 318 11 by by IN work_infoczas7nfbzac5mhjnxpkxle 318 12 LDI LDI NNP work_infoczas7nfbzac5mhjnxpkxle 318 13 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 318 14 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 318 15 in in IN work_infoczas7nfbzac5mhjnxpkxle 318 16 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 318 17 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 318 18 # # NN work_infoczas7nfbzac5mhjnxpkxle 318 19 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 318 20 harboring harbor VBG work_infoczas7nfbzac5mhjnxpkxle 318 21 eight eight CD work_infoczas7nfbzac5mhjnxpkxle 318 22 copies copy NNS work_infoczas7nfbzac5mhjnxpkxle 318 23 of of IN work_infoczas7nfbzac5mhjnxpkxle 318 24 the the DT work_infoczas7nfbzac5mhjnxpkxle 318 25 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 318 26 cassette cassette NN work_infoczas7nfbzac5mhjnxpkxle 318 27 . . . work_infoczas7nfbzac5mhjnxpkxle 319 1 Genomic Genomic NNP work_infoczas7nfbzac5mhjnxpkxle 319 2 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 319 3 was be VBD work_infoczas7nfbzac5mhjnxpkxle 319 4 digested digest VBN work_infoczas7nfbzac5mhjnxpkxle 319 5 with with IN work_infoczas7nfbzac5mhjnxpkxle 319 6 BsrGI BsrGI NNP work_infoczas7nfbzac5mhjnxpkxle 319 7 or or CC work_infoczas7nfbzac5mhjnxpkxle 319 8 BsrGI BsrGI NNP work_infoczas7nfbzac5mhjnxpkxle 319 9 / / SYM work_infoczas7nfbzac5mhjnxpkxle 319 10 NdeI NdeI NNP work_infoczas7nfbzac5mhjnxpkxle 319 11 prior prior RB work_infoczas7nfbzac5mhjnxpkxle 319 12 to to IN work_infoczas7nfbzac5mhjnxpkxle 319 13 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 319 14 amplification amplification NN work_infoczas7nfbzac5mhjnxpkxle 319 15 with with IN work_infoczas7nfbzac5mhjnxpkxle 319 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 319 17 primers primer NNS work_infoczas7nfbzac5mhjnxpkxle 319 18 shown show VBN work_infoczas7nfbzac5mhjnxpkxle 319 19 in in IN work_infoczas7nfbzac5mhjnxpkxle 319 20 Figure figure NN work_infoczas7nfbzac5mhjnxpkxle 319 21 2A. 2a. CD work_infoczas7nfbzac5mhjnxpkxle 320 1 Four four CD work_infoczas7nfbzac5mhjnxpkxle 320 2 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 320 3 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 320 4 sites site NNS work_infoczas7nfbzac5mhjnxpkxle 320 5 , , , work_infoczas7nfbzac5mhjnxpkxle 320 6 all all DT work_infoczas7nfbzac5mhjnxpkxle 320 7 located locate VBN work_infoczas7nfbzac5mhjnxpkxle 320 8 in in IN work_infoczas7nfbzac5mhjnxpkxle 320 9 intergenic intergenic JJ work_infoczas7nfbzac5mhjnxpkxle 320 10 regions region NNS work_infoczas7nfbzac5mhjnxpkxle 320 11 , , , work_infoczas7nfbzac5mhjnxpkxle 320 12 were be VBD work_infoczas7nfbzac5mhjnxpkxle 320 13 identified identify VBN work_infoczas7nfbzac5mhjnxpkxle 320 14 in in IN work_infoczas7nfbzac5mhjnxpkxle 320 15 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 320 16 # # NN work_infoczas7nfbzac5mhjnxpkxle 320 17 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 320 18 by by IN work_infoczas7nfbzac5mhjnxpkxle 320 19 a a DT work_infoczas7nfbzac5mhjnxpkxle 320 20 Blast blast NN work_infoczas7nfbzac5mhjnxpkxle 320 21 searching search VBG work_infoczas7nfbzac5mhjnxpkxle 320 22 the the DT work_infoczas7nfbzac5mhjnxpkxle 320 23 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 320 24 genome genome NN work_infoczas7nfbzac5mhjnxpkxle 320 25 using use VBG work_infoczas7nfbzac5mhjnxpkxle 320 26 sequence sequence NN work_infoczas7nfbzac5mhjnxpkxle 320 27 tags tag NNS work_infoczas7nfbzac5mhjnxpkxle 320 28 identified identify VBN work_infoczas7nfbzac5mhjnxpkxle 320 29 by by IN work_infoczas7nfbzac5mhjnxpkxle 320 30 LDI LDI NNP work_infoczas7nfbzac5mhjnxpkxle 320 31 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 320 32 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 320 33 . . . work_infoczas7nfbzac5mhjnxpkxle 321 1 For for IN work_infoczas7nfbzac5mhjnxpkxle 321 2 one one CD work_infoczas7nfbzac5mhjnxpkxle 321 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 321 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 321 5 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 321 6 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 321 7 indicated indicate VBN work_infoczas7nfbzac5mhjnxpkxle 321 8 with with IN work_infoczas7nfbzac5mhjnxpkxle 321 9 * * NFP work_infoczas7nfbzac5mhjnxpkxle 321 10 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 321 11 , , , work_infoczas7nfbzac5mhjnxpkxle 321 12 only only RB work_infoczas7nfbzac5mhjnxpkxle 321 13 a a DT work_infoczas7nfbzac5mhjnxpkxle 321 14 25-bp 25-bp CD work_infoczas7nfbzac5mhjnxpkxle 321 15 sequence sequence NN work_infoczas7nfbzac5mhjnxpkxle 321 16 tag tag NN work_infoczas7nfbzac5mhjnxpkxle 321 17 could could MD work_infoczas7nfbzac5mhjnxpkxle 321 18 be be VB work_infoczas7nfbzac5mhjnxpkxle 321 19 identified identify VBN work_infoczas7nfbzac5mhjnxpkxle 321 20 due due JJ work_infoczas7nfbzac5mhjnxpkxle 321 21 to to IN work_infoczas7nfbzac5mhjnxpkxle 321 22 the the DT work_infoczas7nfbzac5mhjnxpkxle 321 23 short short JJ work_infoczas7nfbzac5mhjnxpkxle 321 24 length length NN work_infoczas7nfbzac5mhjnxpkxle 321 25 of of IN work_infoczas7nfbzac5mhjnxpkxle 321 26 the the DT work_infoczas7nfbzac5mhjnxpkxle 321 27 LDI LDI NNP work_infoczas7nfbzac5mhjnxpkxle 321 28 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 321 29 PCR PCR NNP work_infoczas7nfbzac5mhjnxpkxle 321 30 product product NN work_infoczas7nfbzac5mhjnxpkxle 321 31 . . . work_infoczas7nfbzac5mhjnxpkxle 322 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 322 2 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 322 3 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 322 4 into into IN work_infoczas7nfbzac5mhjnxpkxle 322 5 chromosome chromosome NN work_infoczas7nfbzac5mhjnxpkxle 322 6 3 3 CD work_infoczas7nfbzac5mhjnxpkxle 322 7 was be VBD work_infoczas7nfbzac5mhjnxpkxle 322 8 flanked flank VBN work_infoczas7nfbzac5mhjnxpkxle 322 9 by by IN work_infoczas7nfbzac5mhjnxpkxle 322 10 a a DT work_infoczas7nfbzac5mhjnxpkxle 322 11 short short JJ work_infoczas7nfbzac5mhjnxpkxle 322 12 stretch stretch NN work_infoczas7nfbzac5mhjnxpkxle 322 13 of of IN work_infoczas7nfbzac5mhjnxpkxle 322 14 TA TA NNP work_infoczas7nfbzac5mhjnxpkxle 322 15 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 322 16 repeats repeat NNS work_infoczas7nfbzac5mhjnxpkxle 322 17 which which WDT work_infoczas7nfbzac5mhjnxpkxle 322 18 represents represent VBZ work_infoczas7nfbzac5mhjnxpkxle 322 19 a a DT work_infoczas7nfbzac5mhjnxpkxle 322 20 potential potential JJ work_infoczas7nfbzac5mhjnxpkxle 322 21 consensus consensus NN work_infoczas7nfbzac5mhjnxpkxle 322 22 SB SB NNP work_infoczas7nfbzac5mhjnxpkxle 322 23 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 322 24 site site NN work_infoczas7nfbzac5mhjnxpkxle 322 25 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 322 26 Vigdal Vigdal NNP work_infoczas7nfbzac5mhjnxpkxle 322 27 et et FW work_infoczas7nfbzac5mhjnxpkxle 322 28 al al NNP work_infoczas7nfbzac5mhjnxpkxle 322 29 . . . work_infoczas7nfbzac5mhjnxpkxle 323 1 2002 2002 CD work_infoczas7nfbzac5mhjnxpkxle 323 2 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 323 3 . . . work_infoczas7nfbzac5mhjnxpkxle 324 1 Figure figure NN work_infoczas7nfbzac5mhjnxpkxle 324 2 4 4 CD work_infoczas7nfbzac5mhjnxpkxle 324 3 . . . work_infoczas7nfbzac5mhjnxpkxle 325 1 Systemic systemic JJ work_infoczas7nfbzac5mhjnxpkxle 325 2 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 325 3 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 325 4 in in IN work_infoczas7nfbzac5mhjnxpkxle 325 5 the the DT work_infoczas7nfbzac5mhjnxpkxle 325 6 SBT SBT NNP work_infoczas7nfbzac5mhjnxpkxle 325 7 / / SYM work_infoczas7nfbzac5mhjnxpkxle 325 8 cHS4.Ubi cHS4.Ubi NNP work_infoczas7nfbzac5mhjnxpkxle 325 9 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 325 10 GIN.cHS4-transgenic GIN.cHS4-transgenic NNP work_infoczas7nfbzac5mhjnxpkxle 325 11 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 325 12 # # NN work_infoczas7nfbzac5mhjnxpkxle 325 13 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 325 14 . . . work_infoczas7nfbzac5mhjnxpkxle 326 1 Organs organ NNS work_infoczas7nfbzac5mhjnxpkxle 326 2 and and CC work_infoczas7nfbzac5mhjnxpkxle 326 3 tissues tissue NNS work_infoczas7nfbzac5mhjnxpkxle 326 4 isolated isolate VBN work_infoczas7nfbzac5mhjnxpkxle 326 5 from from IN work_infoczas7nfbzac5mhjnxpkxle 326 6 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 326 7 # # NN work_infoczas7nfbzac5mhjnxpkxle 326 8 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 326 9 and and CC work_infoczas7nfbzac5mhjnxpkxle 326 10 one one CD work_infoczas7nfbzac5mhjnxpkxle 326 11 wild wild JJ work_infoczas7nfbzac5mhjnxpkxle 326 12 type type NN work_infoczas7nfbzac5mhjnxpkxle 326 13 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 326 14 were be VBD work_infoczas7nfbzac5mhjnxpkxle 326 15 analyzed analyze VBN work_infoczas7nfbzac5mhjnxpkxle 326 16 by by IN work_infoczas7nfbzac5mhjnxpkxle 326 17 normal normal JJ work_infoczas7nfbzac5mhjnxpkxle 326 18 and and CC work_infoczas7nfbzac5mhjnxpkxle 326 19 blue blue JJ work_infoczas7nfbzac5mhjnxpkxle 326 20 light light JJ work_infoczas7nfbzac5mhjnxpkxle 326 21 excitation excitation NN work_infoczas7nfbzac5mhjnxpkxle 326 22 . . . work_infoczas7nfbzac5mhjnxpkxle 327 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 327 2 left left JJ work_infoczas7nfbzac5mhjnxpkxle 327 3 and and CC work_infoczas7nfbzac5mhjnxpkxle 327 4 right right JJ work_infoczas7nfbzac5mhjnxpkxle 327 5 side side NN work_infoczas7nfbzac5mhjnxpkxle 327 6 of of IN work_infoczas7nfbzac5mhjnxpkxle 327 7 each each DT work_infoczas7nfbzac5mhjnxpkxle 327 8 picture picture NN work_infoczas7nfbzac5mhjnxpkxle 327 9 shows show VBZ work_infoczas7nfbzac5mhjnxpkxle 327 10 organ organ NN work_infoczas7nfbzac5mhjnxpkxle 327 11 / / SYM work_infoczas7nfbzac5mhjnxpkxle 327 12 tissue tissue NN work_infoczas7nfbzac5mhjnxpkxle 327 13 from from IN work_infoczas7nfbzac5mhjnxpkxle 327 14 the the DT work_infoczas7nfbzac5mhjnxpkxle 327 15 wild wild JJ work_infoczas7nfbzac5mhjnxpkxle 327 16 type type NN work_infoczas7nfbzac5mhjnxpkxle 327 17 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 327 18 and and CC work_infoczas7nfbzac5mhjnxpkxle 327 19 the the DT work_infoczas7nfbzac5mhjnxpkxle 327 20 pSBT psbt NN work_infoczas7nfbzac5mhjnxpkxle 327 21 / / SYM work_infoczas7nfbzac5mhjnxpkxle 327 22 cHS4.Ubi- cHS4.Ubi- NNP work_infoczas7nfbzac5mhjnxpkxle 327 23 GIN.cHS4 GIN.cHS4 NNP work_infoczas7nfbzac5mhjnxpkxle 327 24 pig pig VB work_infoczas7nfbzac5mhjnxpkxle 327 25 # # NN work_infoczas7nfbzac5mhjnxpkxle 327 26 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 327 27 , , , work_infoczas7nfbzac5mhjnxpkxle 327 28 respectively respectively RB work_infoczas7nfbzac5mhjnxpkxle 327 29 . . . work_infoczas7nfbzac5mhjnxpkxle 328 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 328 2 following follow VBG work_infoczas7nfbzac5mhjnxpkxle 328 3 organs organ NNS work_infoczas7nfbzac5mhjnxpkxle 328 4 or or CC work_infoczas7nfbzac5mhjnxpkxle 328 5 tissues tissue NNS work_infoczas7nfbzac5mhjnxpkxle 328 6 were be VBD work_infoczas7nfbzac5mhjnxpkxle 328 7 analyzed analyze VBN work_infoczas7nfbzac5mhjnxpkxle 328 8 : : : work_infoczas7nfbzac5mhjnxpkxle 328 9 A a DT work_infoczas7nfbzac5mhjnxpkxle 328 10 , , , work_infoczas7nfbzac5mhjnxpkxle 328 11 Brain brain NN work_infoczas7nfbzac5mhjnxpkxle 328 12 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 13 B b NN work_infoczas7nfbzac5mhjnxpkxle 328 14 , , , work_infoczas7nfbzac5mhjnxpkxle 328 15 Rib rib NN work_infoczas7nfbzac5mhjnxpkxle 328 16 bone bone NN work_infoczas7nfbzac5mhjnxpkxle 328 17 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 18 C C NNP work_infoczas7nfbzac5mhjnxpkxle 328 19 , , , work_infoczas7nfbzac5mhjnxpkxle 328 20 Skeletal skeletal JJ work_infoczas7nfbzac5mhjnxpkxle 328 21 muscle muscle NN work_infoczas7nfbzac5mhjnxpkxle 328 22 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 23 D d NN work_infoczas7nfbzac5mhjnxpkxle 328 24 , , , work_infoczas7nfbzac5mhjnxpkxle 328 25 Salivary salivary JJ work_infoczas7nfbzac5mhjnxpkxle 328 26 gland gland NN work_infoczas7nfbzac5mhjnxpkxle 328 27 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 28 E e NN work_infoczas7nfbzac5mhjnxpkxle 328 29 , , , work_infoczas7nfbzac5mhjnxpkxle 328 30 Tongue Tongue NNP work_infoczas7nfbzac5mhjnxpkxle 328 31 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 32 F F NNP work_infoczas7nfbzac5mhjnxpkxle 328 33 , , , work_infoczas7nfbzac5mhjnxpkxle 328 34 Eye Eye NNP work_infoczas7nfbzac5mhjnxpkxle 328 35 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 36 G g NN work_infoczas7nfbzac5mhjnxpkxle 328 37 , , , work_infoczas7nfbzac5mhjnxpkxle 328 38 Heart heart NN work_infoczas7nfbzac5mhjnxpkxle 328 39 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 40 H h NN work_infoczas7nfbzac5mhjnxpkxle 328 41 , , , work_infoczas7nfbzac5mhjnxpkxle 328 42 Testis Testis NNP work_infoczas7nfbzac5mhjnxpkxle 328 43 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 44 I -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 328 45 , , , work_infoczas7nfbzac5mhjnxpkxle 328 46 Subcutaneous subcutaneous JJ work_infoczas7nfbzac5mhjnxpkxle 328 47 fat fat NN work_infoczas7nfbzac5mhjnxpkxle 328 48 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 49 J J NNP work_infoczas7nfbzac5mhjnxpkxle 328 50 , , , work_infoczas7nfbzac5mhjnxpkxle 328 51 Pancreas Pancreas NNP work_infoczas7nfbzac5mhjnxpkxle 328 52 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 53 K K NNP work_infoczas7nfbzac5mhjnxpkxle 328 54 , , , work_infoczas7nfbzac5mhjnxpkxle 328 55 Spleen Spleen NNP work_infoczas7nfbzac5mhjnxpkxle 328 56 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 57 L L NNP work_infoczas7nfbzac5mhjnxpkxle 328 58 , , , work_infoczas7nfbzac5mhjnxpkxle 328 59 Bladder Bladder NNP work_infoczas7nfbzac5mhjnxpkxle 328 60 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 61 M M NNP work_infoczas7nfbzac5mhjnxpkxle 328 62 , , , work_infoczas7nfbzac5mhjnxpkxle 328 63 Colon colon NN work_infoczas7nfbzac5mhjnxpkxle 328 64 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 65 N N NNP work_infoczas7nfbzac5mhjnxpkxle 328 66 , , , work_infoczas7nfbzac5mhjnxpkxle 328 67 Kidney kidney NN work_infoczas7nfbzac5mhjnxpkxle 328 68 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 69 O o UH work_infoczas7nfbzac5mhjnxpkxle 328 70 , , , work_infoczas7nfbzac5mhjnxpkxle 328 71 Lung Lung NNP work_infoczas7nfbzac5mhjnxpkxle 328 72 ; ; : work_infoczas7nfbzac5mhjnxpkxle 328 73 P P NNP work_infoczas7nfbzac5mhjnxpkxle 328 74 , , , work_infoczas7nfbzac5mhjnxpkxle 328 75 Liver Liver NNP work_infoczas7nfbzac5mhjnxpkxle 328 76 , , , work_infoczas7nfbzac5mhjnxpkxle 328 77 and and CC work_infoczas7nfbzac5mhjnxpkxle 328 78 Q Q NNP work_infoczas7nfbzac5mhjnxpkxle 328 79 , , , work_infoczas7nfbzac5mhjnxpkxle 328 80 Skin skin NN work_infoczas7nfbzac5mhjnxpkxle 328 81 . . . work_infoczas7nfbzac5mhjnxpkxle 329 1 Diagrams diagram NNS work_infoczas7nfbzac5mhjnxpkxle 329 2 A1 a1 VBP work_infoczas7nfbzac5mhjnxpkxle 329 3 through through IN work_infoczas7nfbzac5mhjnxpkxle 329 4 Q1 Q1 NNP work_infoczas7nfbzac5mhjnxpkxle 329 5 show show VBP work_infoczas7nfbzac5mhjnxpkxle 329 6 21 21 CD work_infoczas7nfbzac5mhjnxpkxle 329 7 samples sample NNS work_infoczas7nfbzac5mhjnxpkxle 329 8 under under IN work_infoczas7nfbzac5mhjnxpkxle 329 9 normal normal JJ work_infoczas7nfbzac5mhjnxpkxle 329 10 light light NN work_infoczas7nfbzac5mhjnxpkxle 329 11 displayed display VBN work_infoczas7nfbzac5mhjnxpkxle 329 12 in in IN work_infoczas7nfbzac5mhjnxpkxle 329 13 black black JJ work_infoczas7nfbzac5mhjnxpkxle 329 14 and and CC work_infoczas7nfbzac5mhjnxpkxle 329 15 white white JJ work_infoczas7nfbzac5mhjnxpkxle 329 16 . . . work_infoczas7nfbzac5mhjnxpkxle 330 1 A2 A2 NNP work_infoczas7nfbzac5mhjnxpkxle 330 2 through through IN work_infoczas7nfbzac5mhjnxpkxle 330 3 Q2 Q2 NNP work_infoczas7nfbzac5mhjnxpkxle 330 4 show show NN work_infoczas7nfbzac5mhjnxpkxle 330 5 samples sample NNS work_infoczas7nfbzac5mhjnxpkxle 330 6 under under IN work_infoczas7nfbzac5mhjnxpkxle 330 7 blue blue JJ work_infoczas7nfbzac5mhjnxpkxle 330 8 light light JJ work_infoczas7nfbzac5mhjnxpkxle 330 9 excitation excitation NN work_infoczas7nfbzac5mhjnxpkxle 330 10 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 330 11 480 480 CD work_infoczas7nfbzac5mhjnxpkxle 330 12 nm nm CD work_infoczas7nfbzac5mhjnxpkxle 330 13 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 330 14 allowing allow VBG work_infoczas7nfbzac5mhjnxpkxle 330 15 visualization visualization NN work_infoczas7nfbzac5mhjnxpkxle 330 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 330 17 GFP GFP NNP work_infoczas7nfbzac5mhjnxpkxle 330 18 . . . work_infoczas7nfbzac5mhjnxpkxle 331 1 22 22 CD work_infoczas7nfbzac5mhjnxpkxle 331 2 REFERENCES reference NNS work_infoczas7nfbzac5mhjnxpkxle 331 3 Betthauser Betthauser NNP work_infoczas7nfbzac5mhjnxpkxle 331 4 J J NNP work_infoczas7nfbzac5mhjnxpkxle 331 5 , , , work_infoczas7nfbzac5mhjnxpkxle 331 6 Forsberg Forsberg NNP work_infoczas7nfbzac5mhjnxpkxle 331 7 E E NNP work_infoczas7nfbzac5mhjnxpkxle 331 8 , , , work_infoczas7nfbzac5mhjnxpkxle 331 9 Augenstein Augenstein NNP work_infoczas7nfbzac5mhjnxpkxle 331 10 M M NNP work_infoczas7nfbzac5mhjnxpkxle 331 11 , , , work_infoczas7nfbzac5mhjnxpkxle 331 12 Childs Childs NNP work_infoczas7nfbzac5mhjnxpkxle 331 13 L L NNP work_infoczas7nfbzac5mhjnxpkxle 331 14 , , , work_infoczas7nfbzac5mhjnxpkxle 331 15 Eilertsen Eilertsen NNP work_infoczas7nfbzac5mhjnxpkxle 331 16 K K NNP work_infoczas7nfbzac5mhjnxpkxle 331 17 , , , work_infoczas7nfbzac5mhjnxpkxle 331 18 Enos Enos NNP work_infoczas7nfbzac5mhjnxpkxle 331 19 J J NNP work_infoczas7nfbzac5mhjnxpkxle 331 20 , , , work_infoczas7nfbzac5mhjnxpkxle 331 21 Forsythe Forsythe NNP work_infoczas7nfbzac5mhjnxpkxle 331 22 T T NNP work_infoczas7nfbzac5mhjnxpkxle 331 23 , , , work_infoczas7nfbzac5mhjnxpkxle 331 24 Golueke Golueke NNP work_infoczas7nfbzac5mhjnxpkxle 331 25 P P NNP work_infoczas7nfbzac5mhjnxpkxle 331 26 , , , work_infoczas7nfbzac5mhjnxpkxle 331 27 Jurgella Jurgella NNP work_infoczas7nfbzac5mhjnxpkxle 331 28 G G NNP work_infoczas7nfbzac5mhjnxpkxle 331 29 , , , work_infoczas7nfbzac5mhjnxpkxle 331 30 Koppang Koppang NNP work_infoczas7nfbzac5mhjnxpkxle 331 31 R R NNP work_infoczas7nfbzac5mhjnxpkxle 331 32 , , , work_infoczas7nfbzac5mhjnxpkxle 331 33 Lesmeister Lesmeister NNP work_infoczas7nfbzac5mhjnxpkxle 331 34 T T NNP work_infoczas7nfbzac5mhjnxpkxle 331 35 , , , work_infoczas7nfbzac5mhjnxpkxle 331 36 Mallon Mallon NNP work_infoczas7nfbzac5mhjnxpkxle 331 37 K K NNP work_infoczas7nfbzac5mhjnxpkxle 331 38 , , , work_infoczas7nfbzac5mhjnxpkxle 331 39 Mell Mell NNP work_infoczas7nfbzac5mhjnxpkxle 331 40 G G NNP work_infoczas7nfbzac5mhjnxpkxle 331 41 , , , work_infoczas7nfbzac5mhjnxpkxle 331 42 Misica Misica NNP work_infoczas7nfbzac5mhjnxpkxle 331 43 P P NNP work_infoczas7nfbzac5mhjnxpkxle 331 44 , , , work_infoczas7nfbzac5mhjnxpkxle 331 45 Pace Pace NNP work_infoczas7nfbzac5mhjnxpkxle 331 46 M M NNP work_infoczas7nfbzac5mhjnxpkxle 331 47 , , , work_infoczas7nfbzac5mhjnxpkxle 331 48 Pfister Pfister NNP work_infoczas7nfbzac5mhjnxpkxle 331 49 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 331 50 Genskow Genskow NNP work_infoczas7nfbzac5mhjnxpkxle 331 51 M M NNP work_infoczas7nfbzac5mhjnxpkxle 331 52 , , , work_infoczas7nfbzac5mhjnxpkxle 331 53 Strelchenko Strelchenko NNP work_infoczas7nfbzac5mhjnxpkxle 331 54 N N NNP work_infoczas7nfbzac5mhjnxpkxle 331 55 , , , work_infoczas7nfbzac5mhjnxpkxle 331 56 Voelker Voelker NNP work_infoczas7nfbzac5mhjnxpkxle 331 57 G G NNP work_infoczas7nfbzac5mhjnxpkxle 331 58 , , , work_infoczas7nfbzac5mhjnxpkxle 331 59 Watt Watt NNP work_infoczas7nfbzac5mhjnxpkxle 331 60 S S NNP work_infoczas7nfbzac5mhjnxpkxle 331 61 , , , work_infoczas7nfbzac5mhjnxpkxle 331 62 Thompson Thompson NNP work_infoczas7nfbzac5mhjnxpkxle 331 63 S S NNP work_infoczas7nfbzac5mhjnxpkxle 331 64 , , , work_infoczas7nfbzac5mhjnxpkxle 331 65 Bishop Bishop NNP work_infoczas7nfbzac5mhjnxpkxle 331 66 M M NNP work_infoczas7nfbzac5mhjnxpkxle 331 67 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 331 68 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 331 69 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 331 70 Production production NN work_infoczas7nfbzac5mhjnxpkxle 331 71 of of IN work_infoczas7nfbzac5mhjnxpkxle 331 72 cloned clone VBN work_infoczas7nfbzac5mhjnxpkxle 331 73 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 331 74 from from IN work_infoczas7nfbzac5mhjnxpkxle 331 75 in in IN work_infoczas7nfbzac5mhjnxpkxle 331 76 vitro vitro FW work_infoczas7nfbzac5mhjnxpkxle 331 77 systems system NNS work_infoczas7nfbzac5mhjnxpkxle 331 78 . . . work_infoczas7nfbzac5mhjnxpkxle 332 1 Nat Nat NNP work_infoczas7nfbzac5mhjnxpkxle 332 2 Biotechnol Biotechnol NNP work_infoczas7nfbzac5mhjnxpkxle 332 3 18:1055 18:1055 CD work_infoczas7nfbzac5mhjnxpkxle 332 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 332 5 1059 1059 CD work_infoczas7nfbzac5mhjnxpkxle 332 6 . . . work_infoczas7nfbzac5mhjnxpkxle 333 1 Cabot Cabot NNP work_infoczas7nfbzac5mhjnxpkxle 333 2 RA RA NNP work_infoczas7nfbzac5mhjnxpkxle 333 3 , , , work_infoczas7nfbzac5mhjnxpkxle 333 4 Kuhholzer Kuhholzer NNP work_infoczas7nfbzac5mhjnxpkxle 333 5 B B NNP work_infoczas7nfbzac5mhjnxpkxle 333 6 , , , work_infoczas7nfbzac5mhjnxpkxle 333 7 Chan Chan NNP work_infoczas7nfbzac5mhjnxpkxle 333 8 AW AW NNP work_infoczas7nfbzac5mhjnxpkxle 333 9 , , , work_infoczas7nfbzac5mhjnxpkxle 333 10 Lai Lai NNP work_infoczas7nfbzac5mhjnxpkxle 333 11 L L NNP work_infoczas7nfbzac5mhjnxpkxle 333 12 , , , work_infoczas7nfbzac5mhjnxpkxle 333 13 Park Park NNP work_infoczas7nfbzac5mhjnxpkxle 333 14 KW KW NNP work_infoczas7nfbzac5mhjnxpkxle 333 15 , , , work_infoczas7nfbzac5mhjnxpkxle 333 16 Chong Chong NNP work_infoczas7nfbzac5mhjnxpkxle 333 17 KY KY NNP work_infoczas7nfbzac5mhjnxpkxle 333 18 , , , work_infoczas7nfbzac5mhjnxpkxle 333 19 Schatten Schatten NNP work_infoczas7nfbzac5mhjnxpkxle 333 20 G g NN work_infoczas7nfbzac5mhjnxpkxle 333 21 , , , work_infoczas7nfbzac5mhjnxpkxle 333 22 Murphy Murphy NNP work_infoczas7nfbzac5mhjnxpkxle 333 23 CN CN NNP work_infoczas7nfbzac5mhjnxpkxle 333 24 , , , work_infoczas7nfbzac5mhjnxpkxle 333 25 Abeydeera Abeydeera NNP work_infoczas7nfbzac5mhjnxpkxle 333 26 LR LR NNP work_infoczas7nfbzac5mhjnxpkxle 333 27 , , , work_infoczas7nfbzac5mhjnxpkxle 333 28 Day Day NNP work_infoczas7nfbzac5mhjnxpkxle 333 29 BN BN NNP work_infoczas7nfbzac5mhjnxpkxle 333 30 , , , work_infoczas7nfbzac5mhjnxpkxle 333 31 Prather Prather NNP work_infoczas7nfbzac5mhjnxpkxle 333 32 RS RS NNP work_infoczas7nfbzac5mhjnxpkxle 333 33 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 333 34 2001 2001 CD work_infoczas7nfbzac5mhjnxpkxle 333 35 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 333 36 Transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 333 37 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 333 38 produced produce VBN work_infoczas7nfbzac5mhjnxpkxle 333 39 using use VBG work_infoczas7nfbzac5mhjnxpkxle 333 40 in in FW work_infoczas7nfbzac5mhjnxpkxle 333 41 vitro vitro FW work_infoczas7nfbzac5mhjnxpkxle 333 42 matured mature VBN work_infoczas7nfbzac5mhjnxpkxle 333 43 oocytes oocyte NNS work_infoczas7nfbzac5mhjnxpkxle 333 44 infected infect VBN work_infoczas7nfbzac5mhjnxpkxle 333 45 with with IN work_infoczas7nfbzac5mhjnxpkxle 333 46 a a DT work_infoczas7nfbzac5mhjnxpkxle 333 47 retroviral retroviral JJ work_infoczas7nfbzac5mhjnxpkxle 333 48 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 333 49 . . . work_infoczas7nfbzac5mhjnxpkxle 334 1 Anim Anim NNP work_infoczas7nfbzac5mhjnxpkxle 334 2 Biotechnol Biotechnol NNP work_infoczas7nfbzac5mhjnxpkxle 334 3 12:205 12:205 CD work_infoczas7nfbzac5mhjnxpkxle 334 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 334 5 214 214 CD work_infoczas7nfbzac5mhjnxpkxle 334 6 . . . work_infoczas7nfbzac5mhjnxpkxle 335 1 Cibelli Cibelli NNP work_infoczas7nfbzac5mhjnxpkxle 335 2 JB JB NNP work_infoczas7nfbzac5mhjnxpkxle 335 3 , , , work_infoczas7nfbzac5mhjnxpkxle 335 4 Stice Stice NNP work_infoczas7nfbzac5mhjnxpkxle 335 5 SL SL NNP work_infoczas7nfbzac5mhjnxpkxle 335 6 , , , work_infoczas7nfbzac5mhjnxpkxle 335 7 Golueke Golueke NNP work_infoczas7nfbzac5mhjnxpkxle 335 8 PJ PJ NNP work_infoczas7nfbzac5mhjnxpkxle 335 9 , , , work_infoczas7nfbzac5mhjnxpkxle 335 10 Kane Kane NNP work_infoczas7nfbzac5mhjnxpkxle 335 11 JJ JJ NNP work_infoczas7nfbzac5mhjnxpkxle 335 12 , , , work_infoczas7nfbzac5mhjnxpkxle 335 13 Jerry Jerry NNP work_infoczas7nfbzac5mhjnxpkxle 335 14 J J NNP work_infoczas7nfbzac5mhjnxpkxle 335 15 , , , work_infoczas7nfbzac5mhjnxpkxle 335 16 Blackwell Blackwell NNP work_infoczas7nfbzac5mhjnxpkxle 335 17 C C NNP work_infoczas7nfbzac5mhjnxpkxle 335 18 , , , work_infoczas7nfbzac5mhjnxpkxle 335 19 Ponce Ponce NNP work_infoczas7nfbzac5mhjnxpkxle 335 20 de de IN work_infoczas7nfbzac5mhjnxpkxle 335 21 Leon Leon NNP work_infoczas7nfbzac5mhjnxpkxle 335 22 FA FA NNP work_infoczas7nfbzac5mhjnxpkxle 335 23 , , , work_infoczas7nfbzac5mhjnxpkxle 335 24 Robl Robl NNP work_infoczas7nfbzac5mhjnxpkxle 335 25 JM JM NNP work_infoczas7nfbzac5mhjnxpkxle 335 26 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 335 27 1998 1998 CD work_infoczas7nfbzac5mhjnxpkxle 335 28 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 335 29 Cloned clone VBN work_infoczas7nfbzac5mhjnxpkxle 335 30 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 335 31 calves calf NNS work_infoczas7nfbzac5mhjnxpkxle 335 32 produced produce VBN work_infoczas7nfbzac5mhjnxpkxle 335 33 from from IN work_infoczas7nfbzac5mhjnxpkxle 335 34 nonquiescent nonquiescent JJ work_infoczas7nfbzac5mhjnxpkxle 335 35 fetal fetal JJ work_infoczas7nfbzac5mhjnxpkxle 335 36 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 335 37 . . . work_infoczas7nfbzac5mhjnxpkxle 336 1 Science Science NNP work_infoczas7nfbzac5mhjnxpkxle 336 2 280:1256 280:1256 NNP work_infoczas7nfbzac5mhjnxpkxle 336 3 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 336 4 1258 1258 CD work_infoczas7nfbzac5mhjnxpkxle 336 5 . . . work_infoczas7nfbzac5mhjnxpkxle 337 1 Ciuffi Ciuffi NNP work_infoczas7nfbzac5mhjnxpkxle 337 2 A A NNP work_infoczas7nfbzac5mhjnxpkxle 337 3 , , , work_infoczas7nfbzac5mhjnxpkxle 337 4 Llano Llano NNP work_infoczas7nfbzac5mhjnxpkxle 337 5 M M NNP work_infoczas7nfbzac5mhjnxpkxle 337 6 , , , work_infoczas7nfbzac5mhjnxpkxle 337 7 Poeschla Poeschla NNP work_infoczas7nfbzac5mhjnxpkxle 337 8 E E NNP work_infoczas7nfbzac5mhjnxpkxle 337 9 , , , work_infoczas7nfbzac5mhjnxpkxle 337 10 Hoffmann Hoffmann NNP work_infoczas7nfbzac5mhjnxpkxle 337 11 C C NNP work_infoczas7nfbzac5mhjnxpkxle 337 12 , , , work_infoczas7nfbzac5mhjnxpkxle 337 13 Leipzig Leipzig NNP work_infoczas7nfbzac5mhjnxpkxle 337 14 J J NNP work_infoczas7nfbzac5mhjnxpkxle 337 15 , , , work_infoczas7nfbzac5mhjnxpkxle 337 16 Shinn Shinn NNP work_infoczas7nfbzac5mhjnxpkxle 337 17 P P NNP work_infoczas7nfbzac5mhjnxpkxle 337 18 , , , work_infoczas7nfbzac5mhjnxpkxle 337 19 Ecker Ecker NNP work_infoczas7nfbzac5mhjnxpkxle 337 20 JR JR NNP work_infoczas7nfbzac5mhjnxpkxle 337 21 , , , work_infoczas7nfbzac5mhjnxpkxle 337 22 Bushman Bushman NNP work_infoczas7nfbzac5mhjnxpkxle 337 23 F F NNP work_infoczas7nfbzac5mhjnxpkxle 337 24 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 337 25 2005 2005 CD work_infoczas7nfbzac5mhjnxpkxle 337 26 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 337 27 A a DT work_infoczas7nfbzac5mhjnxpkxle 337 28 role role NN work_infoczas7nfbzac5mhjnxpkxle 337 29 for for IN work_infoczas7nfbzac5mhjnxpkxle 337 30 LEDGF LEDGF NNP work_infoczas7nfbzac5mhjnxpkxle 337 31 / / SYM work_infoczas7nfbzac5mhjnxpkxle 337 32 p75 p75 NN work_infoczas7nfbzac5mhjnxpkxle 337 33 in in IN work_infoczas7nfbzac5mhjnxpkxle 337 34 targeting target VBG work_infoczas7nfbzac5mhjnxpkxle 337 35 HIV HIV NNP work_infoczas7nfbzac5mhjnxpkxle 337 36 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 337 37 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 337 38 . . . work_infoczas7nfbzac5mhjnxpkxle 338 1 Nat Nat NNP work_infoczas7nfbzac5mhjnxpkxle 338 2 Med Med NNP work_infoczas7nfbzac5mhjnxpkxle 338 3 11:1287 11:1287 CD work_infoczas7nfbzac5mhjnxpkxle 338 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 338 5 1289 1289 CD work_infoczas7nfbzac5mhjnxpkxle 338 6 . . . work_infoczas7nfbzac5mhjnxpkxle 339 1 Clark Clark NNP work_infoczas7nfbzac5mhjnxpkxle 339 2 KJ KJ NNP work_infoczas7nfbzac5mhjnxpkxle 339 3 , , , work_infoczas7nfbzac5mhjnxpkxle 339 4 Carlson Carlson NNP work_infoczas7nfbzac5mhjnxpkxle 339 5 DF DF NNP work_infoczas7nfbzac5mhjnxpkxle 339 6 , , , work_infoczas7nfbzac5mhjnxpkxle 339 7 Fahrenkrug Fahrenkrug NNP work_infoczas7nfbzac5mhjnxpkxle 339 8 SC SC NNP work_infoczas7nfbzac5mhjnxpkxle 339 9 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 339 10 2007a 2007a CD work_infoczas7nfbzac5mhjnxpkxle 339 11 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 339 12 Pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 339 13 taking take VBG work_infoczas7nfbzac5mhjnxpkxle 339 14 wing wing NN work_infoczas7nfbzac5mhjnxpkxle 339 15 with with IN work_infoczas7nfbzac5mhjnxpkxle 339 16 transposons transposon NNS work_infoczas7nfbzac5mhjnxpkxle 339 17 and and CC work_infoczas7nfbzac5mhjnxpkxle 339 18 recombinases recombinase NNS work_infoczas7nfbzac5mhjnxpkxle 339 19 . . . work_infoczas7nfbzac5mhjnxpkxle 340 1 Genome Genome NNP work_infoczas7nfbzac5mhjnxpkxle 340 2 Biol Biol NNP work_infoczas7nfbzac5mhjnxpkxle 340 3 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 340 4 Suppl Suppl NNP work_infoczas7nfbzac5mhjnxpkxle 340 5 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 340 6 : : : work_infoczas7nfbzac5mhjnxpkxle 340 7 S13 s13 NN work_infoczas7nfbzac5mhjnxpkxle 340 8 . . . work_infoczas7nfbzac5mhjnxpkxle 341 1 Clark Clark NNP work_infoczas7nfbzac5mhjnxpkxle 341 2 KJ KJ NNP work_infoczas7nfbzac5mhjnxpkxle 341 3 , , , work_infoczas7nfbzac5mhjnxpkxle 341 4 Carlson Carlson NNP work_infoczas7nfbzac5mhjnxpkxle 341 5 DF DF NNP work_infoczas7nfbzac5mhjnxpkxle 341 6 , , , work_infoczas7nfbzac5mhjnxpkxle 341 7 Foster Foster NNP work_infoczas7nfbzac5mhjnxpkxle 341 8 LK LK NNP work_infoczas7nfbzac5mhjnxpkxle 341 9 , , , work_infoczas7nfbzac5mhjnxpkxle 341 10 Kong Kong NNP work_infoczas7nfbzac5mhjnxpkxle 341 11 BW BW NNP work_infoczas7nfbzac5mhjnxpkxle 341 12 , , , work_infoczas7nfbzac5mhjnxpkxle 341 13 Foster Foster NNP work_infoczas7nfbzac5mhjnxpkxle 341 14 DN DN NNP work_infoczas7nfbzac5mhjnxpkxle 341 15 , , , work_infoczas7nfbzac5mhjnxpkxle 341 16 Fahrenkrug Fahrenkrug NNP work_infoczas7nfbzac5mhjnxpkxle 341 17 SC SC NNP work_infoczas7nfbzac5mhjnxpkxle 341 18 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 341 19 2007b 2007b NNS work_infoczas7nfbzac5mhjnxpkxle 341 20 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 341 21 Enzymatic enzymatic JJ work_infoczas7nfbzac5mhjnxpkxle 341 22 engineering engineering NN work_infoczas7nfbzac5mhjnxpkxle 341 23 of of IN work_infoczas7nfbzac5mhjnxpkxle 341 24 the the DT work_infoczas7nfbzac5mhjnxpkxle 341 25 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 341 26 genome genome NN work_infoczas7nfbzac5mhjnxpkxle 341 27 with with IN work_infoczas7nfbzac5mhjnxpkxle 341 28 transposons transposon NNS work_infoczas7nfbzac5mhjnxpkxle 341 29 and and CC work_infoczas7nfbzac5mhjnxpkxle 341 30 recombinases recombinase NNS work_infoczas7nfbzac5mhjnxpkxle 341 31 . . . work_infoczas7nfbzac5mhjnxpkxle 342 1 BMC BMC NNP work_infoczas7nfbzac5mhjnxpkxle 342 2 Biotechnol Biotechnol NNP work_infoczas7nfbzac5mhjnxpkxle 342 3 7:42 7:42 CD work_infoczas7nfbzac5mhjnxpkxle 342 4 . . . work_infoczas7nfbzac5mhjnxpkxle 343 1 Dalsgaard Dalsgaard NNP work_infoczas7nfbzac5mhjnxpkxle 343 2 T T NNP work_infoczas7nfbzac5mhjnxpkxle 343 3 , , , work_infoczas7nfbzac5mhjnxpkxle 343 4 Moldt Moldt NNP work_infoczas7nfbzac5mhjnxpkxle 343 5 B B NNP work_infoczas7nfbzac5mhjnxpkxle 343 6 , , , work_infoczas7nfbzac5mhjnxpkxle 343 7 Sharma Sharma NNP work_infoczas7nfbzac5mhjnxpkxle 343 8 N N NNP work_infoczas7nfbzac5mhjnxpkxle 343 9 , , , work_infoczas7nfbzac5mhjnxpkxle 343 10 Wolf Wolf NNP work_infoczas7nfbzac5mhjnxpkxle 343 11 G G NNP work_infoczas7nfbzac5mhjnxpkxle 343 12 , , , work_infoczas7nfbzac5mhjnxpkxle 343 13 Schmitz Schmitz NNP work_infoczas7nfbzac5mhjnxpkxle 343 14 A A NNP work_infoczas7nfbzac5mhjnxpkxle 343 15 , , , work_infoczas7nfbzac5mhjnxpkxle 343 16 Pedersen Pedersen NNP work_infoczas7nfbzac5mhjnxpkxle 343 17 FS FS NNP work_infoczas7nfbzac5mhjnxpkxle 343 18 , , , work_infoczas7nfbzac5mhjnxpkxle 343 19 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 343 20 JG JG NNP work_infoczas7nfbzac5mhjnxpkxle 343 21 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 343 22 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 343 23 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 343 24 Shielding shield VBG work_infoczas7nfbzac5mhjnxpkxle 343 25 of of IN work_infoczas7nfbzac5mhjnxpkxle 343 26 sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 343 27 beauty beauty NN work_infoczas7nfbzac5mhjnxpkxle 343 28 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 343 29 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 343 30 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 343 31 delivered deliver VBN work_infoczas7nfbzac5mhjnxpkxle 343 32 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 343 33 cassettes cassette NNS work_infoczas7nfbzac5mhjnxpkxle 343 34 by by IN work_infoczas7nfbzac5mhjnxpkxle 343 35 heterologous heterologous JJ work_infoczas7nfbzac5mhjnxpkxle 343 36 insulators insulator NNS work_infoczas7nfbzac5mhjnxpkxle 343 37 in in IN work_infoczas7nfbzac5mhjnxpkxle 343 38 early early JJ work_infoczas7nfbzac5mhjnxpkxle 343 39 embryonal embryonal JJ work_infoczas7nfbzac5mhjnxpkxle 343 40 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 343 41 . . . work_infoczas7nfbzac5mhjnxpkxle 344 1 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 344 2 Ther Ther NNP work_infoczas7nfbzac5mhjnxpkxle 344 3 17:121 17:121 CD work_infoczas7nfbzac5mhjnxpkxle 344 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 344 5 130 130 CD work_infoczas7nfbzac5mhjnxpkxle 344 6 . . . work_infoczas7nfbzac5mhjnxpkxle 345 1 Davidson Davidson NNP work_infoczas7nfbzac5mhjnxpkxle 345 2 AE AE NNP work_infoczas7nfbzac5mhjnxpkxle 345 3 , , , work_infoczas7nfbzac5mhjnxpkxle 345 4 Balciunas Balciunas NNP work_infoczas7nfbzac5mhjnxpkxle 345 5 D D NNP work_infoczas7nfbzac5mhjnxpkxle 345 6 , , , work_infoczas7nfbzac5mhjnxpkxle 345 7 Mohn Mohn NNP work_infoczas7nfbzac5mhjnxpkxle 345 8 D D NNP work_infoczas7nfbzac5mhjnxpkxle 345 9 , , , work_infoczas7nfbzac5mhjnxpkxle 345 10 Shaffer Shaffer NNP work_infoczas7nfbzac5mhjnxpkxle 345 11 J J NNP work_infoczas7nfbzac5mhjnxpkxle 345 12 , , , work_infoczas7nfbzac5mhjnxpkxle 345 13 Hermanson Hermanson NNP work_infoczas7nfbzac5mhjnxpkxle 345 14 S S NNP work_infoczas7nfbzac5mhjnxpkxle 345 15 , , , work_infoczas7nfbzac5mhjnxpkxle 345 16 Sivasubbu Sivasubbu NNP work_infoczas7nfbzac5mhjnxpkxle 345 17 S S NNP work_infoczas7nfbzac5mhjnxpkxle 345 18 , , , work_infoczas7nfbzac5mhjnxpkxle 345 19 Cliff Cliff NNP work_infoczas7nfbzac5mhjnxpkxle 345 20 MP MP NNP work_infoczas7nfbzac5mhjnxpkxle 345 21 , , , work_infoczas7nfbzac5mhjnxpkxle 345 22 Hackett Hackett NNP work_infoczas7nfbzac5mhjnxpkxle 345 23 PB PB NNP work_infoczas7nfbzac5mhjnxpkxle 345 24 , , , work_infoczas7nfbzac5mhjnxpkxle 345 25 Ekker Ekker NNP work_infoczas7nfbzac5mhjnxpkxle 345 26 SC SC NNP work_infoczas7nfbzac5mhjnxpkxle 345 27 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 345 28 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 345 29 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 345 30 Efficient efficient JJ work_infoczas7nfbzac5mhjnxpkxle 345 31 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 345 32 delivery delivery NN work_infoczas7nfbzac5mhjnxpkxle 345 33 and and CC work_infoczas7nfbzac5mhjnxpkxle 345 34 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 345 35 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 345 36 in in IN work_infoczas7nfbzac5mhjnxpkxle 345 37 zebrafish zebrafish NNP work_infoczas7nfbzac5mhjnxpkxle 345 38 using use VBG work_infoczas7nfbzac5mhjnxpkxle 345 39 the the DT work_infoczas7nfbzac5mhjnxpkxle 345 40 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 345 41 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 345 42 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 345 43 . . . work_infoczas7nfbzac5mhjnxpkxle 346 1 Dev Dev NNP work_infoczas7nfbzac5mhjnxpkxle 346 2 Biol Biol NNP work_infoczas7nfbzac5mhjnxpkxle 346 3 263:191 263:191 CD work_infoczas7nfbzac5mhjnxpkxle 346 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 346 5 202 202 CD work_infoczas7nfbzac5mhjnxpkxle 346 6 . . . work_infoczas7nfbzac5mhjnxpkxle 347 1 23 23 CD work_infoczas7nfbzac5mhjnxpkxle 347 2 Du Du NNP work_infoczas7nfbzac5mhjnxpkxle 347 3 Y Y NNP work_infoczas7nfbzac5mhjnxpkxle 347 4 , , , work_infoczas7nfbzac5mhjnxpkxle 347 5 Kragh Kragh NNP work_infoczas7nfbzac5mhjnxpkxle 347 6 PM PM NNP work_infoczas7nfbzac5mhjnxpkxle 347 7 , , , work_infoczas7nfbzac5mhjnxpkxle 347 8 Zhang Zhang NNP work_infoczas7nfbzac5mhjnxpkxle 347 9 Y Y NNP work_infoczas7nfbzac5mhjnxpkxle 347 10 , , , work_infoczas7nfbzac5mhjnxpkxle 347 11 Li Li NNP work_infoczas7nfbzac5mhjnxpkxle 347 12 J J NNP work_infoczas7nfbzac5mhjnxpkxle 347 13 , , , work_infoczas7nfbzac5mhjnxpkxle 347 14 Schmidt Schmidt NNP work_infoczas7nfbzac5mhjnxpkxle 347 15 M M NNP work_infoczas7nfbzac5mhjnxpkxle 347 16 , , , work_infoczas7nfbzac5mhjnxpkxle 347 17 Bogh Bogh NNP work_infoczas7nfbzac5mhjnxpkxle 347 18 IB IB NNP work_infoczas7nfbzac5mhjnxpkxle 347 19 , , , work_infoczas7nfbzac5mhjnxpkxle 347 20 Zhang Zhang NNP work_infoczas7nfbzac5mhjnxpkxle 347 21 X X NNP work_infoczas7nfbzac5mhjnxpkxle 347 22 , , , work_infoczas7nfbzac5mhjnxpkxle 347 23 Purup Purup NNP work_infoczas7nfbzac5mhjnxpkxle 347 24 S S NNP work_infoczas7nfbzac5mhjnxpkxle 347 25 , , , work_infoczas7nfbzac5mhjnxpkxle 347 26 Jorgensen Jorgensen NNP work_infoczas7nfbzac5mhjnxpkxle 347 27 AL AL NNP work_infoczas7nfbzac5mhjnxpkxle 347 28 , , , work_infoczas7nfbzac5mhjnxpkxle 347 29 Pedersen Pedersen NNP work_infoczas7nfbzac5mhjnxpkxle 347 30 AM am NN work_infoczas7nfbzac5mhjnxpkxle 347 31 , , , work_infoczas7nfbzac5mhjnxpkxle 347 32 Villemoes Villemoes NNP work_infoczas7nfbzac5mhjnxpkxle 347 33 K K NNP work_infoczas7nfbzac5mhjnxpkxle 347 34 , , , work_infoczas7nfbzac5mhjnxpkxle 347 35 Yang Yang NNP work_infoczas7nfbzac5mhjnxpkxle 347 36 H H NNP work_infoczas7nfbzac5mhjnxpkxle 347 37 , , , work_infoczas7nfbzac5mhjnxpkxle 347 38 Bolund Bolund NNP work_infoczas7nfbzac5mhjnxpkxle 347 39 L L NNP work_infoczas7nfbzac5mhjnxpkxle 347 40 , , , work_infoczas7nfbzac5mhjnxpkxle 347 41 Vajta Vajta NNP work_infoczas7nfbzac5mhjnxpkxle 347 42 G G NNP work_infoczas7nfbzac5mhjnxpkxle 347 43 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 347 44 2007 2007 CD work_infoczas7nfbzac5mhjnxpkxle 347 45 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 347 46 Piglets piglet NNS work_infoczas7nfbzac5mhjnxpkxle 347 47 born bear VBN work_infoczas7nfbzac5mhjnxpkxle 347 48 from from IN work_infoczas7nfbzac5mhjnxpkxle 347 49 handmade handmade JJ work_infoczas7nfbzac5mhjnxpkxle 347 50 cloning cloning NN work_infoczas7nfbzac5mhjnxpkxle 347 51 , , , work_infoczas7nfbzac5mhjnxpkxle 347 52 an an DT work_infoczas7nfbzac5mhjnxpkxle 347 53 innovative innovative JJ work_infoczas7nfbzac5mhjnxpkxle 347 54 cloning cloning NN work_infoczas7nfbzac5mhjnxpkxle 347 55 method method NN work_infoczas7nfbzac5mhjnxpkxle 347 56 without without IN work_infoczas7nfbzac5mhjnxpkxle 347 57 micromanipulation micromanipulation NN work_infoczas7nfbzac5mhjnxpkxle 347 58 . . . work_infoczas7nfbzac5mhjnxpkxle 348 1 Theriogenology Theriogenology NNP work_infoczas7nfbzac5mhjnxpkxle 348 2 68:1104 68:1104 CD work_infoczas7nfbzac5mhjnxpkxle 348 3 - - SYM work_infoczas7nfbzac5mhjnxpkxle 348 4 1110 1110 CD work_infoczas7nfbzac5mhjnxpkxle 348 5 . . . work_infoczas7nfbzac5mhjnxpkxle 349 1 Dupuy Dupuy NNP work_infoczas7nfbzac5mhjnxpkxle 349 2 AJ AJ NNP work_infoczas7nfbzac5mhjnxpkxle 349 3 , , , work_infoczas7nfbzac5mhjnxpkxle 349 4 Clark Clark NNP work_infoczas7nfbzac5mhjnxpkxle 349 5 K K NNP work_infoczas7nfbzac5mhjnxpkxle 349 6 , , , work_infoczas7nfbzac5mhjnxpkxle 349 7 Carlson Carlson NNP work_infoczas7nfbzac5mhjnxpkxle 349 8 CM CM NNP work_infoczas7nfbzac5mhjnxpkxle 349 9 , , , work_infoczas7nfbzac5mhjnxpkxle 349 10 Fritz Fritz NNP work_infoczas7nfbzac5mhjnxpkxle 349 11 S S NNP work_infoczas7nfbzac5mhjnxpkxle 349 12 , , , work_infoczas7nfbzac5mhjnxpkxle 349 13 Davidson Davidson NNP work_infoczas7nfbzac5mhjnxpkxle 349 14 AE AE NNP work_infoczas7nfbzac5mhjnxpkxle 349 15 , , , work_infoczas7nfbzac5mhjnxpkxle 349 16 Markley Markley NNP work_infoczas7nfbzac5mhjnxpkxle 349 17 KM KM NNP work_infoczas7nfbzac5mhjnxpkxle 349 18 , , , work_infoczas7nfbzac5mhjnxpkxle 349 19 Finley Finley NNP work_infoczas7nfbzac5mhjnxpkxle 349 20 K K NNP work_infoczas7nfbzac5mhjnxpkxle 349 21 , , , work_infoczas7nfbzac5mhjnxpkxle 349 22 Fletcher Fletcher NNP work_infoczas7nfbzac5mhjnxpkxle 349 23 CF CF NNP work_infoczas7nfbzac5mhjnxpkxle 349 24 , , , work_infoczas7nfbzac5mhjnxpkxle 349 25 Ekker Ekker NNP work_infoczas7nfbzac5mhjnxpkxle 349 26 SC SC NNP work_infoczas7nfbzac5mhjnxpkxle 349 27 , , , work_infoczas7nfbzac5mhjnxpkxle 349 28 Hackett Hackett NNP work_infoczas7nfbzac5mhjnxpkxle 349 29 PB PB NNP work_infoczas7nfbzac5mhjnxpkxle 349 30 , , , work_infoczas7nfbzac5mhjnxpkxle 349 31 Horn Horn NNP work_infoczas7nfbzac5mhjnxpkxle 349 32 S S NNP work_infoczas7nfbzac5mhjnxpkxle 349 33 , , , work_infoczas7nfbzac5mhjnxpkxle 349 34 Largaespada Largaespada NNP work_infoczas7nfbzac5mhjnxpkxle 349 35 DA DA NNP work_infoczas7nfbzac5mhjnxpkxle 349 36 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 349 37 2002 2002 CD work_infoczas7nfbzac5mhjnxpkxle 349 38 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 349 39 Mammalian mammalian JJ work_infoczas7nfbzac5mhjnxpkxle 349 40 germ germ NN work_infoczas7nfbzac5mhjnxpkxle 349 41 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 349 42 line line NN work_infoczas7nfbzac5mhjnxpkxle 349 43 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 349 44 by by IN work_infoczas7nfbzac5mhjnxpkxle 349 45 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 349 46 . . . work_infoczas7nfbzac5mhjnxpkxle 350 1 Proc Proc NNP work_infoczas7nfbzac5mhjnxpkxle 350 2 Natl Natl NNP work_infoczas7nfbzac5mhjnxpkxle 350 3 Acad Acad NNP work_infoczas7nfbzac5mhjnxpkxle 350 4 Sci Sci NNP work_infoczas7nfbzac5mhjnxpkxle 350 5 U U NNP work_infoczas7nfbzac5mhjnxpkxle 350 6 S S NNP work_infoczas7nfbzac5mhjnxpkxle 350 7 A A NNP work_infoczas7nfbzac5mhjnxpkxle 350 8 99:4495 99:4495 CD work_infoczas7nfbzac5mhjnxpkxle 350 9 - - SYM work_infoczas7nfbzac5mhjnxpkxle 350 10 4499 4499 CD work_infoczas7nfbzac5mhjnxpkxle 350 11 . . . work_infoczas7nfbzac5mhjnxpkxle 351 1 Garrick Garrick NNP work_infoczas7nfbzac5mhjnxpkxle 351 2 D D NNP work_infoczas7nfbzac5mhjnxpkxle 351 3 , , , work_infoczas7nfbzac5mhjnxpkxle 351 4 Fiering Fiering NNP work_infoczas7nfbzac5mhjnxpkxle 351 5 S S NNP work_infoczas7nfbzac5mhjnxpkxle 351 6 , , , work_infoczas7nfbzac5mhjnxpkxle 351 7 Martin Martin NNP work_infoczas7nfbzac5mhjnxpkxle 351 8 DI DI NNP work_infoczas7nfbzac5mhjnxpkxle 351 9 , , , work_infoczas7nfbzac5mhjnxpkxle 351 10 Whitelaw Whitelaw NNP work_infoczas7nfbzac5mhjnxpkxle 351 11 E e NN work_infoczas7nfbzac5mhjnxpkxle 351 12 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 351 13 1998 1998 CD work_infoczas7nfbzac5mhjnxpkxle 351 14 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 351 15 Repeat repeat NN work_infoczas7nfbzac5mhjnxpkxle 351 16 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 351 17 induced induce VBN work_infoczas7nfbzac5mhjnxpkxle 351 18 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 351 19 silencing silencing NN work_infoczas7nfbzac5mhjnxpkxle 351 20 in in IN work_infoczas7nfbzac5mhjnxpkxle 351 21 mammals mammal NNS work_infoczas7nfbzac5mhjnxpkxle 351 22 . . . work_infoczas7nfbzac5mhjnxpkxle 352 1 Nat Nat NNP work_infoczas7nfbzac5mhjnxpkxle 352 2 Genet Genet NNP work_infoczas7nfbzac5mhjnxpkxle 352 3 18:56 18:56 CD work_infoczas7nfbzac5mhjnxpkxle 352 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 352 5 59 59 CD work_infoczas7nfbzac5mhjnxpkxle 352 6 . . . work_infoczas7nfbzac5mhjnxpkxle 353 1 Hammer Hammer NNP work_infoczas7nfbzac5mhjnxpkxle 353 2 RE RE NNP work_infoczas7nfbzac5mhjnxpkxle 353 3 , , , work_infoczas7nfbzac5mhjnxpkxle 353 4 Pursel Pursel NNP work_infoczas7nfbzac5mhjnxpkxle 353 5 VG VG NNP work_infoczas7nfbzac5mhjnxpkxle 353 6 , , , work_infoczas7nfbzac5mhjnxpkxle 353 7 Rexroad Rexroad NNP work_infoczas7nfbzac5mhjnxpkxle 353 8 CE CE NNP work_infoczas7nfbzac5mhjnxpkxle 353 9 , , , work_infoczas7nfbzac5mhjnxpkxle 353 10 Jr. Jr. NNP work_infoczas7nfbzac5mhjnxpkxle 353 11 , , , work_infoczas7nfbzac5mhjnxpkxle 353 12 Wall Wall NNP work_infoczas7nfbzac5mhjnxpkxle 353 13 RJ RJ NNP work_infoczas7nfbzac5mhjnxpkxle 353 14 , , , work_infoczas7nfbzac5mhjnxpkxle 353 15 Bolt Bolt NNP work_infoczas7nfbzac5mhjnxpkxle 353 16 DJ DJ NNP work_infoczas7nfbzac5mhjnxpkxle 353 17 , , , work_infoczas7nfbzac5mhjnxpkxle 353 18 Ebert Ebert NNP work_infoczas7nfbzac5mhjnxpkxle 353 19 KM KM NNP work_infoczas7nfbzac5mhjnxpkxle 353 20 , , , work_infoczas7nfbzac5mhjnxpkxle 353 21 Palmiter Palmiter NNP work_infoczas7nfbzac5mhjnxpkxle 353 22 RD RD NNP work_infoczas7nfbzac5mhjnxpkxle 353 23 , , , work_infoczas7nfbzac5mhjnxpkxle 353 24 Brinster Brinster NNP work_infoczas7nfbzac5mhjnxpkxle 353 25 RL RL NNP work_infoczas7nfbzac5mhjnxpkxle 353 26 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 353 27 1985 1985 CD work_infoczas7nfbzac5mhjnxpkxle 353 28 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 353 29 Production production NN work_infoczas7nfbzac5mhjnxpkxle 353 30 of of IN work_infoczas7nfbzac5mhjnxpkxle 353 31 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 353 32 rabbits rabbit NNS work_infoczas7nfbzac5mhjnxpkxle 353 33 , , , work_infoczas7nfbzac5mhjnxpkxle 353 34 sheep sheep NNS work_infoczas7nfbzac5mhjnxpkxle 353 35 and and CC work_infoczas7nfbzac5mhjnxpkxle 353 36 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 353 37 by by IN work_infoczas7nfbzac5mhjnxpkxle 353 38 microinjection microinjection NN work_infoczas7nfbzac5mhjnxpkxle 353 39 . . . work_infoczas7nfbzac5mhjnxpkxle 354 1 Nature nature NN work_infoczas7nfbzac5mhjnxpkxle 354 2 315:680 315:680 CD work_infoczas7nfbzac5mhjnxpkxle 354 3 - - SYM work_infoczas7nfbzac5mhjnxpkxle 354 4 683 683 CD work_infoczas7nfbzac5mhjnxpkxle 354 5 . . . work_infoczas7nfbzac5mhjnxpkxle 355 1 Hirabayashi Hirabayashi NNP work_infoczas7nfbzac5mhjnxpkxle 355 2 M M NNP work_infoczas7nfbzac5mhjnxpkxle 355 3 , , , work_infoczas7nfbzac5mhjnxpkxle 355 4 Takahashi Takahashi NNP work_infoczas7nfbzac5mhjnxpkxle 355 5 R R NNP work_infoczas7nfbzac5mhjnxpkxle 355 6 , , , work_infoczas7nfbzac5mhjnxpkxle 355 7 Ito Ito NNP work_infoczas7nfbzac5mhjnxpkxle 355 8 K K NNP work_infoczas7nfbzac5mhjnxpkxle 355 9 , , , work_infoczas7nfbzac5mhjnxpkxle 355 10 Kashiwazaki Kashiwazaki NNP work_infoczas7nfbzac5mhjnxpkxle 355 11 N N NNP work_infoczas7nfbzac5mhjnxpkxle 355 12 , , , work_infoczas7nfbzac5mhjnxpkxle 355 13 Hirao Hirao NNP work_infoczas7nfbzac5mhjnxpkxle 355 14 M M NNP work_infoczas7nfbzac5mhjnxpkxle 355 15 , , , work_infoczas7nfbzac5mhjnxpkxle 355 16 Hirasawa Hirasawa NNP work_infoczas7nfbzac5mhjnxpkxle 355 17 K K NNP work_infoczas7nfbzac5mhjnxpkxle 355 18 , , , work_infoczas7nfbzac5mhjnxpkxle 355 19 Hochi Hochi NNP work_infoczas7nfbzac5mhjnxpkxle 355 20 S S NNP work_infoczas7nfbzac5mhjnxpkxle 355 21 , , , work_infoczas7nfbzac5mhjnxpkxle 355 22 Ueda Ueda NNP work_infoczas7nfbzac5mhjnxpkxle 355 23 M M NNP work_infoczas7nfbzac5mhjnxpkxle 355 24 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 355 25 2001 2001 CD work_infoczas7nfbzac5mhjnxpkxle 355 26 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 355 27 A a DT work_infoczas7nfbzac5mhjnxpkxle 355 28 comparative comparative JJ work_infoczas7nfbzac5mhjnxpkxle 355 29 study study NN work_infoczas7nfbzac5mhjnxpkxle 355 30 on on IN work_infoczas7nfbzac5mhjnxpkxle 355 31 the the DT work_infoczas7nfbzac5mhjnxpkxle 355 32 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 355 33 of of IN work_infoczas7nfbzac5mhjnxpkxle 355 34 exogenous exogenous JJ work_infoczas7nfbzac5mhjnxpkxle 355 35 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 355 36 into into IN work_infoczas7nfbzac5mhjnxpkxle 355 37 mouse mouse NN work_infoczas7nfbzac5mhjnxpkxle 355 38 , , , work_infoczas7nfbzac5mhjnxpkxle 355 39 rat rat NN work_infoczas7nfbzac5mhjnxpkxle 355 40 , , , work_infoczas7nfbzac5mhjnxpkxle 355 41 rabbit rabbit NN work_infoczas7nfbzac5mhjnxpkxle 355 42 , , , work_infoczas7nfbzac5mhjnxpkxle 355 43 and and CC work_infoczas7nfbzac5mhjnxpkxle 355 44 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 355 45 genomes genome NNS work_infoczas7nfbzac5mhjnxpkxle 355 46 . . . work_infoczas7nfbzac5mhjnxpkxle 356 1 Exp Exp NNP work_infoczas7nfbzac5mhjnxpkxle 356 2 Anim Anim NNP work_infoczas7nfbzac5mhjnxpkxle 356 3 50:125 50:125 CD work_infoczas7nfbzac5mhjnxpkxle 356 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 356 5 131 131 CD work_infoczas7nfbzac5mhjnxpkxle 356 6 . . . work_infoczas7nfbzac5mhjnxpkxle 357 1 Hofmann Hofmann NNP work_infoczas7nfbzac5mhjnxpkxle 357 2 A A NNP work_infoczas7nfbzac5mhjnxpkxle 357 3 , , , work_infoczas7nfbzac5mhjnxpkxle 357 4 Kessler Kessler NNP work_infoczas7nfbzac5mhjnxpkxle 357 5 B B NNP work_infoczas7nfbzac5mhjnxpkxle 357 6 , , , work_infoczas7nfbzac5mhjnxpkxle 357 7 Ewerling Ewerling NNP work_infoczas7nfbzac5mhjnxpkxle 357 8 S S NNP work_infoczas7nfbzac5mhjnxpkxle 357 9 , , , work_infoczas7nfbzac5mhjnxpkxle 357 10 Weppert Weppert NNP work_infoczas7nfbzac5mhjnxpkxle 357 11 M M NNP work_infoczas7nfbzac5mhjnxpkxle 357 12 , , , work_infoczas7nfbzac5mhjnxpkxle 357 13 Vogg Vogg NNP work_infoczas7nfbzac5mhjnxpkxle 357 14 B B NNP work_infoczas7nfbzac5mhjnxpkxle 357 15 , , , work_infoczas7nfbzac5mhjnxpkxle 357 16 Ludwig Ludwig NNP work_infoczas7nfbzac5mhjnxpkxle 357 17 H H NNP work_infoczas7nfbzac5mhjnxpkxle 357 18 , , , work_infoczas7nfbzac5mhjnxpkxle 357 19 Stojkovic Stojkovic NNP work_infoczas7nfbzac5mhjnxpkxle 357 20 M M NNP work_infoczas7nfbzac5mhjnxpkxle 357 21 , , , work_infoczas7nfbzac5mhjnxpkxle 357 22 Boelhauve Boelhauve NNP work_infoczas7nfbzac5mhjnxpkxle 357 23 M M NNP work_infoczas7nfbzac5mhjnxpkxle 357 24 , , , work_infoczas7nfbzac5mhjnxpkxle 357 25 Brem Brem NNP work_infoczas7nfbzac5mhjnxpkxle 357 26 G g NN work_infoczas7nfbzac5mhjnxpkxle 357 27 , , , work_infoczas7nfbzac5mhjnxpkxle 357 28 Wolf Wolf NNP work_infoczas7nfbzac5mhjnxpkxle 357 29 E E NNP work_infoczas7nfbzac5mhjnxpkxle 357 30 , , , work_infoczas7nfbzac5mhjnxpkxle 357 31 Pfeifer Pfeifer NNP work_infoczas7nfbzac5mhjnxpkxle 357 32 A a NN work_infoczas7nfbzac5mhjnxpkxle 357 33 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 357 34 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 357 35 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 357 36 Efficient efficient JJ work_infoczas7nfbzac5mhjnxpkxle 357 37 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 357 38 in in IN work_infoczas7nfbzac5mhjnxpkxle 357 39 farm farm NN work_infoczas7nfbzac5mhjnxpkxle 357 40 animals animal NNS work_infoczas7nfbzac5mhjnxpkxle 357 41 by by IN work_infoczas7nfbzac5mhjnxpkxle 357 42 lentiviral lentiviral JJ work_infoczas7nfbzac5mhjnxpkxle 357 43 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 357 44 . . . work_infoczas7nfbzac5mhjnxpkxle 358 1 EMBO EMBO NNP work_infoczas7nfbzac5mhjnxpkxle 358 2 Rep Rep NNP work_infoczas7nfbzac5mhjnxpkxle 358 3 4:1054 4:1054 CD work_infoczas7nfbzac5mhjnxpkxle 358 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 358 5 1060 1060 CD work_infoczas7nfbzac5mhjnxpkxle 358 6 . . . work_infoczas7nfbzac5mhjnxpkxle 359 1 Hyun Hyun NNP work_infoczas7nfbzac5mhjnxpkxle 359 2 S S NNP work_infoczas7nfbzac5mhjnxpkxle 359 3 , , , work_infoczas7nfbzac5mhjnxpkxle 359 4 Lee Lee NNP work_infoczas7nfbzac5mhjnxpkxle 359 5 G G NNP work_infoczas7nfbzac5mhjnxpkxle 359 6 , , , work_infoczas7nfbzac5mhjnxpkxle 359 7 Kim Kim NNP work_infoczas7nfbzac5mhjnxpkxle 359 8 D D NNP work_infoczas7nfbzac5mhjnxpkxle 359 9 , , , work_infoczas7nfbzac5mhjnxpkxle 359 10 Kim Kim NNP work_infoczas7nfbzac5mhjnxpkxle 359 11 H H NNP work_infoczas7nfbzac5mhjnxpkxle 359 12 , , , work_infoczas7nfbzac5mhjnxpkxle 359 13 Lee Lee NNP work_infoczas7nfbzac5mhjnxpkxle 359 14 S S NNP work_infoczas7nfbzac5mhjnxpkxle 359 15 , , , work_infoczas7nfbzac5mhjnxpkxle 359 16 Nam Nam NNP work_infoczas7nfbzac5mhjnxpkxle 359 17 D D NNP work_infoczas7nfbzac5mhjnxpkxle 359 18 , , , work_infoczas7nfbzac5mhjnxpkxle 359 19 Jeong Jeong NNP work_infoczas7nfbzac5mhjnxpkxle 359 20 Y Y NNP work_infoczas7nfbzac5mhjnxpkxle 359 21 , , , work_infoczas7nfbzac5mhjnxpkxle 359 22 Kim Kim NNP work_infoczas7nfbzac5mhjnxpkxle 359 23 S S NNP work_infoczas7nfbzac5mhjnxpkxle 359 24 , , , work_infoczas7nfbzac5mhjnxpkxle 359 25 Yeom Yeom NNP work_infoczas7nfbzac5mhjnxpkxle 359 26 S S NNP work_infoczas7nfbzac5mhjnxpkxle 359 27 , , , work_infoczas7nfbzac5mhjnxpkxle 359 28 Kang Kang NNP work_infoczas7nfbzac5mhjnxpkxle 359 29 S S NNP work_infoczas7nfbzac5mhjnxpkxle 359 30 , , , work_infoczas7nfbzac5mhjnxpkxle 359 31 Han Han NNP work_infoczas7nfbzac5mhjnxpkxle 359 32 J J NNP work_infoczas7nfbzac5mhjnxpkxle 359 33 , , , work_infoczas7nfbzac5mhjnxpkxle 359 34 Lee Lee NNP work_infoczas7nfbzac5mhjnxpkxle 359 35 B B NNP work_infoczas7nfbzac5mhjnxpkxle 359 36 , , , work_infoczas7nfbzac5mhjnxpkxle 359 37 Hwang Hwang NNP work_infoczas7nfbzac5mhjnxpkxle 359 38 W W NNP work_infoczas7nfbzac5mhjnxpkxle 359 39 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 359 40 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 359 41 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 359 42 Production production NN work_infoczas7nfbzac5mhjnxpkxle 359 43 of of IN work_infoczas7nfbzac5mhjnxpkxle 359 44 nuclear nuclear JJ work_infoczas7nfbzac5mhjnxpkxle 359 45 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 359 46 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 359 47 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 359 48 piglets piglet NNS work_infoczas7nfbzac5mhjnxpkxle 359 49 using use VBG work_infoczas7nfbzac5mhjnxpkxle 359 50 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 359 51 fetal fetal JJ work_infoczas7nfbzac5mhjnxpkxle 359 52 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 359 53 transfected transfecte VBN work_infoczas7nfbzac5mhjnxpkxle 359 54 with with IN work_infoczas7nfbzac5mhjnxpkxle 359 55 the the DT work_infoczas7nfbzac5mhjnxpkxle 359 56 enhanced enhanced JJ work_infoczas7nfbzac5mhjnxpkxle 359 57 green green JJ work_infoczas7nfbzac5mhjnxpkxle 359 58 fluorescent fluorescent NN work_infoczas7nfbzac5mhjnxpkxle 359 59 protein protein NN work_infoczas7nfbzac5mhjnxpkxle 359 60 . . . work_infoczas7nfbzac5mhjnxpkxle 360 1 Biol Biol NNP work_infoczas7nfbzac5mhjnxpkxle 360 2 Reprod Reprod NNP work_infoczas7nfbzac5mhjnxpkxle 360 3 69:1060 69:1060 CD work_infoczas7nfbzac5mhjnxpkxle 360 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 360 5 1068 1068 CD work_infoczas7nfbzac5mhjnxpkxle 360 6 . . . work_infoczas7nfbzac5mhjnxpkxle 361 1 Ivics Ivics NNP work_infoczas7nfbzac5mhjnxpkxle 361 2 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 361 3 , , , work_infoczas7nfbzac5mhjnxpkxle 361 4 Hackett Hackett NNP work_infoczas7nfbzac5mhjnxpkxle 361 5 PB PB NNP work_infoczas7nfbzac5mhjnxpkxle 361 6 , , , work_infoczas7nfbzac5mhjnxpkxle 361 7 Plasterk Plasterk NNP work_infoczas7nfbzac5mhjnxpkxle 361 8 RH RH NNP work_infoczas7nfbzac5mhjnxpkxle 361 9 , , , work_infoczas7nfbzac5mhjnxpkxle 361 10 Izsvak Izsvak NNP work_infoczas7nfbzac5mhjnxpkxle 361 11 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 361 12 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 361 13 1997 1997 CD work_infoczas7nfbzac5mhjnxpkxle 361 14 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 361 15 Molecular molecular JJ work_infoczas7nfbzac5mhjnxpkxle 361 16 reconstruction reconstruction NN work_infoczas7nfbzac5mhjnxpkxle 361 17 of of IN work_infoczas7nfbzac5mhjnxpkxle 361 18 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 361 19 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 361 20 , , , work_infoczas7nfbzac5mhjnxpkxle 361 21 a a DT work_infoczas7nfbzac5mhjnxpkxle 361 22 Tc1-like tc1-like JJ work_infoczas7nfbzac5mhjnxpkxle 361 23 transposon transposon NN work_infoczas7nfbzac5mhjnxpkxle 361 24 from from IN work_infoczas7nfbzac5mhjnxpkxle 361 25 fish fish NN work_infoczas7nfbzac5mhjnxpkxle 361 26 , , , work_infoczas7nfbzac5mhjnxpkxle 361 27 and and CC work_infoczas7nfbzac5mhjnxpkxle 361 28 its -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 361 29 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 361 30 in in IN work_infoczas7nfbzac5mhjnxpkxle 361 31 human human JJ work_infoczas7nfbzac5mhjnxpkxle 361 32 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 361 33 . . . work_infoczas7nfbzac5mhjnxpkxle 362 1 Cell Cell NNP work_infoczas7nfbzac5mhjnxpkxle 362 2 91:501 91:501 CD work_infoczas7nfbzac5mhjnxpkxle 362 3 - - : work_infoczas7nfbzac5mhjnxpkxle 362 4 510 510 CD work_infoczas7nfbzac5mhjnxpkxle 362 5 . . . work_infoczas7nfbzac5mhjnxpkxle 363 1 Izsvak Izsvak NNP work_infoczas7nfbzac5mhjnxpkxle 363 2 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 363 3 , , , work_infoczas7nfbzac5mhjnxpkxle 363 4 Ivics Ivics NNP work_infoczas7nfbzac5mhjnxpkxle 363 5 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 363 6 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 363 7 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 363 8 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 363 9 Sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 363 10 beauty beauty NN work_infoczas7nfbzac5mhjnxpkxle 363 11 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 363 12 : : : work_infoczas7nfbzac5mhjnxpkxle 363 13 biology biology NN work_infoczas7nfbzac5mhjnxpkxle 363 14 and and CC work_infoczas7nfbzac5mhjnxpkxle 363 15 applications application NNS work_infoczas7nfbzac5mhjnxpkxle 363 16 for for IN work_infoczas7nfbzac5mhjnxpkxle 363 17 molecular molecular JJ work_infoczas7nfbzac5mhjnxpkxle 363 18 therapy therapy NN work_infoczas7nfbzac5mhjnxpkxle 363 19 . . . work_infoczas7nfbzac5mhjnxpkxle 364 1 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 364 2 Ther Ther NNP work_infoczas7nfbzac5mhjnxpkxle 364 3 9:147 9:147 NNP work_infoczas7nfbzac5mhjnxpkxle 364 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 364 5 156 156 CD work_infoczas7nfbzac5mhjnxpkxle 364 6 . . . work_infoczas7nfbzac5mhjnxpkxle 365 1 24 24 CD work_infoczas7nfbzac5mhjnxpkxle 365 2 Izsvak Izsvak NNP work_infoczas7nfbzac5mhjnxpkxle 365 3 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 365 4 , , , work_infoczas7nfbzac5mhjnxpkxle 365 5 Ivics Ivics NNP work_infoczas7nfbzac5mhjnxpkxle 365 6 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 365 7 , , , work_infoczas7nfbzac5mhjnxpkxle 365 8 Plasterk Plasterk NNP work_infoczas7nfbzac5mhjnxpkxle 365 9 RH RH NNP work_infoczas7nfbzac5mhjnxpkxle 365 10 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 365 11 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 365 12 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 365 13 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 365 14 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 365 15 , , , work_infoczas7nfbzac5mhjnxpkxle 365 16 a a DT work_infoczas7nfbzac5mhjnxpkxle 365 17 wide wide JJ work_infoczas7nfbzac5mhjnxpkxle 365 18 host host NN work_infoczas7nfbzac5mhjnxpkxle 365 19 - - JJ work_infoczas7nfbzac5mhjnxpkxle 365 20 range range JJ work_infoczas7nfbzac5mhjnxpkxle 365 21 transposon transposon JJ work_infoczas7nfbzac5mhjnxpkxle 365 22 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 365 23 for for IN work_infoczas7nfbzac5mhjnxpkxle 365 24 genetic genetic JJ work_infoczas7nfbzac5mhjnxpkxle 365 25 transformation transformation NN work_infoczas7nfbzac5mhjnxpkxle 365 26 in in IN work_infoczas7nfbzac5mhjnxpkxle 365 27 vertebrates vertebrate NNS work_infoczas7nfbzac5mhjnxpkxle 365 28 . . . work_infoczas7nfbzac5mhjnxpkxle 366 1 J J NNP work_infoczas7nfbzac5mhjnxpkxle 366 2 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 366 3 Biol Biol NNP work_infoczas7nfbzac5mhjnxpkxle 366 4 302:93 302:93 CD work_infoczas7nfbzac5mhjnxpkxle 366 5 - - SYM work_infoczas7nfbzac5mhjnxpkxle 366 6 102 102 CD work_infoczas7nfbzac5mhjnxpkxle 366 7 . . . work_infoczas7nfbzac5mhjnxpkxle 367 1 Kato Kato NNP work_infoczas7nfbzac5mhjnxpkxle 367 2 Y Y NNP work_infoczas7nfbzac5mhjnxpkxle 367 3 , , , work_infoczas7nfbzac5mhjnxpkxle 367 4 Tani Tani NNP work_infoczas7nfbzac5mhjnxpkxle 367 5 T T NNP work_infoczas7nfbzac5mhjnxpkxle 367 6 , , , work_infoczas7nfbzac5mhjnxpkxle 367 7 Sotomaru Sotomaru NNP work_infoczas7nfbzac5mhjnxpkxle 367 8 Y Y NNP work_infoczas7nfbzac5mhjnxpkxle 367 9 , , , work_infoczas7nfbzac5mhjnxpkxle 367 10 Kurokawa Kurokawa NNP work_infoczas7nfbzac5mhjnxpkxle 367 11 K K NNP work_infoczas7nfbzac5mhjnxpkxle 367 12 , , , work_infoczas7nfbzac5mhjnxpkxle 367 13 Kato Kato NNP work_infoczas7nfbzac5mhjnxpkxle 367 14 J J NNP work_infoczas7nfbzac5mhjnxpkxle 367 15 , , , work_infoczas7nfbzac5mhjnxpkxle 367 16 Doguchi Doguchi NNP work_infoczas7nfbzac5mhjnxpkxle 367 17 H H NNP work_infoczas7nfbzac5mhjnxpkxle 367 18 , , , work_infoczas7nfbzac5mhjnxpkxle 367 19 Yasue Yasue NNP work_infoczas7nfbzac5mhjnxpkxle 367 20 H H NNP work_infoczas7nfbzac5mhjnxpkxle 367 21 , , , work_infoczas7nfbzac5mhjnxpkxle 367 22 Tsunoda Tsunoda NNP work_infoczas7nfbzac5mhjnxpkxle 367 23 Y Y NNP work_infoczas7nfbzac5mhjnxpkxle 367 24 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 367 25 1998 1998 CD work_infoczas7nfbzac5mhjnxpkxle 367 26 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 367 27 Eight eight CD work_infoczas7nfbzac5mhjnxpkxle 367 28 calves calf NNS work_infoczas7nfbzac5mhjnxpkxle 367 29 cloned clone VBN work_infoczas7nfbzac5mhjnxpkxle 367 30 from from IN work_infoczas7nfbzac5mhjnxpkxle 367 31 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 367 32 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 367 33 of of IN work_infoczas7nfbzac5mhjnxpkxle 367 34 a a DT work_infoczas7nfbzac5mhjnxpkxle 367 35 single single JJ work_infoczas7nfbzac5mhjnxpkxle 367 36 adult adult NN work_infoczas7nfbzac5mhjnxpkxle 367 37 . . . work_infoczas7nfbzac5mhjnxpkxle 368 1 Science Science NNP work_infoczas7nfbzac5mhjnxpkxle 368 2 282:2095 282:2095 NNP work_infoczas7nfbzac5mhjnxpkxle 368 3 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 368 4 2098 2098 CD work_infoczas7nfbzac5mhjnxpkxle 368 5 . . . work_infoczas7nfbzac5mhjnxpkxle 369 1 Kragh Kragh NNP work_infoczas7nfbzac5mhjnxpkxle 369 2 PM PM NNP work_infoczas7nfbzac5mhjnxpkxle 369 3 , , , work_infoczas7nfbzac5mhjnxpkxle 369 4 Nielsen Nielsen NNP work_infoczas7nfbzac5mhjnxpkxle 369 5 AL AL NNP work_infoczas7nfbzac5mhjnxpkxle 369 6 , , , work_infoczas7nfbzac5mhjnxpkxle 369 7 Li Li NNP work_infoczas7nfbzac5mhjnxpkxle 369 8 J J NNP work_infoczas7nfbzac5mhjnxpkxle 369 9 , , , work_infoczas7nfbzac5mhjnxpkxle 369 10 Du Du NNP work_infoczas7nfbzac5mhjnxpkxle 369 11 Y Y NNP work_infoczas7nfbzac5mhjnxpkxle 369 12 , , , work_infoczas7nfbzac5mhjnxpkxle 369 13 Lin Lin NNP work_infoczas7nfbzac5mhjnxpkxle 369 14 L L NNP work_infoczas7nfbzac5mhjnxpkxle 369 15 , , , work_infoczas7nfbzac5mhjnxpkxle 369 16 Schmidt Schmidt NNP work_infoczas7nfbzac5mhjnxpkxle 369 17 M M NNP work_infoczas7nfbzac5mhjnxpkxle 369 18 , , , work_infoczas7nfbzac5mhjnxpkxle 369 19 Bogh Bogh NNP work_infoczas7nfbzac5mhjnxpkxle 369 20 IB IB NNP work_infoczas7nfbzac5mhjnxpkxle 369 21 , , , work_infoczas7nfbzac5mhjnxpkxle 369 22 Holm Holm NNP work_infoczas7nfbzac5mhjnxpkxle 369 23 IE IE NNP work_infoczas7nfbzac5mhjnxpkxle 369 24 , , , work_infoczas7nfbzac5mhjnxpkxle 369 25 Jakobsen Jakobsen NNP work_infoczas7nfbzac5mhjnxpkxle 369 26 JE JE NNP work_infoczas7nfbzac5mhjnxpkxle 369 27 , , , work_infoczas7nfbzac5mhjnxpkxle 369 28 Johansen Johansen NNP work_infoczas7nfbzac5mhjnxpkxle 369 29 MG MG NNP work_infoczas7nfbzac5mhjnxpkxle 369 30 , , , work_infoczas7nfbzac5mhjnxpkxle 369 31 Purup Purup NNP work_infoczas7nfbzac5mhjnxpkxle 369 32 S S NNP work_infoczas7nfbzac5mhjnxpkxle 369 33 , , , work_infoczas7nfbzac5mhjnxpkxle 369 34 Bolund Bolund NNP work_infoczas7nfbzac5mhjnxpkxle 369 35 L L NNP work_infoczas7nfbzac5mhjnxpkxle 369 36 , , , work_infoczas7nfbzac5mhjnxpkxle 369 37 Vajta Vajta NNP work_infoczas7nfbzac5mhjnxpkxle 369 38 G G NNP work_infoczas7nfbzac5mhjnxpkxle 369 39 , , , work_infoczas7nfbzac5mhjnxpkxle 369 40 Jorgensen Jorgensen NNP work_infoczas7nfbzac5mhjnxpkxle 369 41 AL AL NNP work_infoczas7nfbzac5mhjnxpkxle 369 42 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 369 43 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 369 44 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 369 45 Hemizygous hemizygous JJ work_infoczas7nfbzac5mhjnxpkxle 369 46 minipigs minipig NNS work_infoczas7nfbzac5mhjnxpkxle 369 47 produced produce VBN work_infoczas7nfbzac5mhjnxpkxle 369 48 by by IN work_infoczas7nfbzac5mhjnxpkxle 369 49 random random JJ work_infoczas7nfbzac5mhjnxpkxle 369 50 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 369 51 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 369 52 and and CC work_infoczas7nfbzac5mhjnxpkxle 369 53 handmade handmade NN work_infoczas7nfbzac5mhjnxpkxle 369 54 cloning cloning NN work_infoczas7nfbzac5mhjnxpkxle 369 55 express express VB work_infoczas7nfbzac5mhjnxpkxle 369 56 the the DT work_infoczas7nfbzac5mhjnxpkxle 369 57 Alzheimer Alzheimer NNP work_infoczas7nfbzac5mhjnxpkxle 369 58 's 's POS work_infoczas7nfbzac5mhjnxpkxle 369 59 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 369 60 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 369 61 causing cause VBG work_infoczas7nfbzac5mhjnxpkxle 369 62 dominant dominant JJ work_infoczas7nfbzac5mhjnxpkxle 369 63 mutation mutation NN work_infoczas7nfbzac5mhjnxpkxle 369 64 APPsw APPsw NNP work_infoczas7nfbzac5mhjnxpkxle 369 65 . . . work_infoczas7nfbzac5mhjnxpkxle 370 1 Transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 370 2 Res Res NNP work_infoczas7nfbzac5mhjnxpkxle 370 3 18:545 18:545 NN work_infoczas7nfbzac5mhjnxpkxle 370 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 370 5 558 558 CD work_infoczas7nfbzac5mhjnxpkxle 370 6 . . . work_infoczas7nfbzac5mhjnxpkxle 371 1 Kragh Kragh NNP work_infoczas7nfbzac5mhjnxpkxle 371 2 PM PM NNP work_infoczas7nfbzac5mhjnxpkxle 371 3 , , , work_infoczas7nfbzac5mhjnxpkxle 371 4 Vajta Vajta NNP work_infoczas7nfbzac5mhjnxpkxle 371 5 G G NNP work_infoczas7nfbzac5mhjnxpkxle 371 6 , , , work_infoczas7nfbzac5mhjnxpkxle 371 7 Corydon Corydon NNP work_infoczas7nfbzac5mhjnxpkxle 371 8 TJ TJ NNP work_infoczas7nfbzac5mhjnxpkxle 371 9 , , , work_infoczas7nfbzac5mhjnxpkxle 371 10 Purup Purup NNP work_infoczas7nfbzac5mhjnxpkxle 371 11 S S NNP work_infoczas7nfbzac5mhjnxpkxle 371 12 , , , work_infoczas7nfbzac5mhjnxpkxle 371 13 Bolund Bolund NNP work_infoczas7nfbzac5mhjnxpkxle 371 14 L L NNP work_infoczas7nfbzac5mhjnxpkxle 371 15 , , , work_infoczas7nfbzac5mhjnxpkxle 371 16 Callesen Callesen NNP work_infoczas7nfbzac5mhjnxpkxle 371 17 H H NNP work_infoczas7nfbzac5mhjnxpkxle 371 18 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 371 19 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 371 20 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 371 21 Production production NN work_infoczas7nfbzac5mhjnxpkxle 371 22 of of IN work_infoczas7nfbzac5mhjnxpkxle 371 23 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 371 24 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 371 25 blastocysts blastocyst NNS work_infoczas7nfbzac5mhjnxpkxle 371 26 by by IN work_infoczas7nfbzac5mhjnxpkxle 371 27 hand hand NN work_infoczas7nfbzac5mhjnxpkxle 371 28 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 371 29 made make VBN work_infoczas7nfbzac5mhjnxpkxle 371 30 cloning cloning NN work_infoczas7nfbzac5mhjnxpkxle 371 31 . . . work_infoczas7nfbzac5mhjnxpkxle 372 1 Reprod Reprod NNP work_infoczas7nfbzac5mhjnxpkxle 372 2 Fertil Fertil NNP work_infoczas7nfbzac5mhjnxpkxle 372 3 Dev Dev NNP work_infoczas7nfbzac5mhjnxpkxle 372 4 16:315 16:315 CD work_infoczas7nfbzac5mhjnxpkxle 372 5 - - SYM work_infoczas7nfbzac5mhjnxpkxle 372 6 318 318 CD work_infoczas7nfbzac5mhjnxpkxle 372 7 . . . work_infoczas7nfbzac5mhjnxpkxle 373 1 Kubota Kubota NNP work_infoczas7nfbzac5mhjnxpkxle 373 2 C C NNP work_infoczas7nfbzac5mhjnxpkxle 373 3 , , , work_infoczas7nfbzac5mhjnxpkxle 373 4 Yamakuchi Yamakuchi NNP work_infoczas7nfbzac5mhjnxpkxle 373 5 H H NNP work_infoczas7nfbzac5mhjnxpkxle 373 6 , , , work_infoczas7nfbzac5mhjnxpkxle 373 7 Todoroki Todoroki NNP work_infoczas7nfbzac5mhjnxpkxle 373 8 J J NNP work_infoczas7nfbzac5mhjnxpkxle 373 9 , , , work_infoczas7nfbzac5mhjnxpkxle 373 10 Mizoshita Mizoshita NNP work_infoczas7nfbzac5mhjnxpkxle 373 11 K K NNP work_infoczas7nfbzac5mhjnxpkxle 373 12 , , , work_infoczas7nfbzac5mhjnxpkxle 373 13 Tabara Tabara NNP work_infoczas7nfbzac5mhjnxpkxle 373 14 N N NNP work_infoczas7nfbzac5mhjnxpkxle 373 15 , , , work_infoczas7nfbzac5mhjnxpkxle 373 16 Barber Barber NNP work_infoczas7nfbzac5mhjnxpkxle 373 17 M M NNP work_infoczas7nfbzac5mhjnxpkxle 373 18 , , , work_infoczas7nfbzac5mhjnxpkxle 373 19 Yang Yang NNP work_infoczas7nfbzac5mhjnxpkxle 373 20 X X NNP work_infoczas7nfbzac5mhjnxpkxle 373 21 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 373 22 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 373 23 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 373 24 Six six CD work_infoczas7nfbzac5mhjnxpkxle 373 25 cloned clone VBN work_infoczas7nfbzac5mhjnxpkxle 373 26 calves calf NNS work_infoczas7nfbzac5mhjnxpkxle 373 27 produced produce VBN work_infoczas7nfbzac5mhjnxpkxle 373 28 from from IN work_infoczas7nfbzac5mhjnxpkxle 373 29 adult adult NN work_infoczas7nfbzac5mhjnxpkxle 373 30 fibroblast fibroblast NN work_infoczas7nfbzac5mhjnxpkxle 373 31 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 373 32 after after IN work_infoczas7nfbzac5mhjnxpkxle 373 33 long long JJ work_infoczas7nfbzac5mhjnxpkxle 373 34 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 373 35 term term NN work_infoczas7nfbzac5mhjnxpkxle 373 36 culture culture NN work_infoczas7nfbzac5mhjnxpkxle 373 37 . . . work_infoczas7nfbzac5mhjnxpkxle 374 1 Proc Proc NNP work_infoczas7nfbzac5mhjnxpkxle 374 2 Natl Natl NNP work_infoczas7nfbzac5mhjnxpkxle 374 3 Acad Acad NNP work_infoczas7nfbzac5mhjnxpkxle 374 4 Sci Sci NNP work_infoczas7nfbzac5mhjnxpkxle 374 5 U U NNP work_infoczas7nfbzac5mhjnxpkxle 374 6 S S NNP work_infoczas7nfbzac5mhjnxpkxle 374 7 A a DT work_infoczas7nfbzac5mhjnxpkxle 374 8 97:990 97:990 CD work_infoczas7nfbzac5mhjnxpkxle 374 9 - - SYM work_infoczas7nfbzac5mhjnxpkxle 374 10 995 995 CD work_infoczas7nfbzac5mhjnxpkxle 374 11 . . . work_infoczas7nfbzac5mhjnxpkxle 375 1 Lai Lai NNP work_infoczas7nfbzac5mhjnxpkxle 375 2 L L NNP work_infoczas7nfbzac5mhjnxpkxle 375 3 , , , work_infoczas7nfbzac5mhjnxpkxle 375 4 Park Park NNP work_infoczas7nfbzac5mhjnxpkxle 375 5 KW KW NNP work_infoczas7nfbzac5mhjnxpkxle 375 6 , , , work_infoczas7nfbzac5mhjnxpkxle 375 7 Cheong Cheong NNP work_infoczas7nfbzac5mhjnxpkxle 375 8 HT HT NNP work_infoczas7nfbzac5mhjnxpkxle 375 9 , , , work_infoczas7nfbzac5mhjnxpkxle 375 10 Kuhholzer Kuhholzer NNP work_infoczas7nfbzac5mhjnxpkxle 375 11 B B NNP work_infoczas7nfbzac5mhjnxpkxle 375 12 , , , work_infoczas7nfbzac5mhjnxpkxle 375 13 Samuel Samuel NNP work_infoczas7nfbzac5mhjnxpkxle 375 14 M M NNP work_infoczas7nfbzac5mhjnxpkxle 375 15 , , , work_infoczas7nfbzac5mhjnxpkxle 375 16 Bonk Bonk NNP work_infoczas7nfbzac5mhjnxpkxle 375 17 A A NNP work_infoczas7nfbzac5mhjnxpkxle 375 18 , , , work_infoczas7nfbzac5mhjnxpkxle 375 19 I -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 375 20 m be VBP work_infoczas7nfbzac5mhjnxpkxle 375 21 GS GS NNP work_infoczas7nfbzac5mhjnxpkxle 375 22 , , , work_infoczas7nfbzac5mhjnxpkxle 375 23 Rieke Rieke NNP work_infoczas7nfbzac5mhjnxpkxle 375 24 A A NNP work_infoczas7nfbzac5mhjnxpkxle 375 25 , , , work_infoczas7nfbzac5mhjnxpkxle 375 26 Day Day NNP work_infoczas7nfbzac5mhjnxpkxle 375 27 BN BN NNP work_infoczas7nfbzac5mhjnxpkxle 375 28 , , , work_infoczas7nfbzac5mhjnxpkxle 375 29 Murphy Murphy NNP work_infoczas7nfbzac5mhjnxpkxle 375 30 CN CN NNP work_infoczas7nfbzac5mhjnxpkxle 375 31 , , , work_infoczas7nfbzac5mhjnxpkxle 375 32 Carter Carter NNP work_infoczas7nfbzac5mhjnxpkxle 375 33 DB DB NNP work_infoczas7nfbzac5mhjnxpkxle 375 34 , , , work_infoczas7nfbzac5mhjnxpkxle 375 35 Prather Prather NNP work_infoczas7nfbzac5mhjnxpkxle 375 36 RS RS NNP work_infoczas7nfbzac5mhjnxpkxle 375 37 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 375 38 2002 2002 CD work_infoczas7nfbzac5mhjnxpkxle 375 39 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 375 40 Transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 375 41 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 375 42 expressing express VBG work_infoczas7nfbzac5mhjnxpkxle 375 43 the the DT work_infoczas7nfbzac5mhjnxpkxle 375 44 enhanced enhanced JJ work_infoczas7nfbzac5mhjnxpkxle 375 45 green green JJ work_infoczas7nfbzac5mhjnxpkxle 375 46 fluorescent fluorescent NN work_infoczas7nfbzac5mhjnxpkxle 375 47 protein protein NN work_infoczas7nfbzac5mhjnxpkxle 375 48 produced produce VBN work_infoczas7nfbzac5mhjnxpkxle 375 49 by by IN work_infoczas7nfbzac5mhjnxpkxle 375 50 nuclear nuclear JJ work_infoczas7nfbzac5mhjnxpkxle 375 51 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 375 52 using use VBG work_infoczas7nfbzac5mhjnxpkxle 375 53 colchicine colchicine NN work_infoczas7nfbzac5mhjnxpkxle 375 54 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 375 55 treated treat VBN work_infoczas7nfbzac5mhjnxpkxle 375 56 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 375 57 as as IN work_infoczas7nfbzac5mhjnxpkxle 375 58 donor donor NN work_infoczas7nfbzac5mhjnxpkxle 375 59 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 375 60 . . . work_infoczas7nfbzac5mhjnxpkxle 376 1 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 376 2 Reprod Reprod NNP work_infoczas7nfbzac5mhjnxpkxle 376 3 Dev Dev NNP work_infoczas7nfbzac5mhjnxpkxle 376 4 62:300 62:300 CD work_infoczas7nfbzac5mhjnxpkxle 376 5 - - SYM work_infoczas7nfbzac5mhjnxpkxle 376 6 306 306 CD work_infoczas7nfbzac5mhjnxpkxle 376 7 . . . work_infoczas7nfbzac5mhjnxpkxle 377 1 Leahy Leahy NNP work_infoczas7nfbzac5mhjnxpkxle 377 2 P P NNP work_infoczas7nfbzac5mhjnxpkxle 377 3 , , , work_infoczas7nfbzac5mhjnxpkxle 377 4 Carmichael Carmichael NNP work_infoczas7nfbzac5mhjnxpkxle 377 5 GG GG NNP work_infoczas7nfbzac5mhjnxpkxle 377 6 , , , work_infoczas7nfbzac5mhjnxpkxle 377 7 Rossomando Rossomando NNP work_infoczas7nfbzac5mhjnxpkxle 377 8 EF EF NNP work_infoczas7nfbzac5mhjnxpkxle 377 9 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 377 10 1997 1997 CD work_infoczas7nfbzac5mhjnxpkxle 377 11 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 377 12 Transcription transcription NN work_infoczas7nfbzac5mhjnxpkxle 377 13 from from IN work_infoczas7nfbzac5mhjnxpkxle 377 14 plasmid plasmid JJ work_infoczas7nfbzac5mhjnxpkxle 377 15 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 377 16 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 377 17 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 377 18 increased increase VBN work_infoczas7nfbzac5mhjnxpkxle 377 19 up up IN work_infoczas7nfbzac5mhjnxpkxle 377 20 to to IN work_infoczas7nfbzac5mhjnxpkxle 377 21 14-fold 14-fold CD work_infoczas7nfbzac5mhjnxpkxle 377 22 when when WRB work_infoczas7nfbzac5mhjnxpkxle 377 23 plasmids plasmid NNS work_infoczas7nfbzac5mhjnxpkxle 377 24 are be VBP work_infoczas7nfbzac5mhjnxpkxle 377 25 transfected transfecte VBN work_infoczas7nfbzac5mhjnxpkxle 377 26 as as IN work_infoczas7nfbzac5mhjnxpkxle 377 27 concatemers concatemer NNS work_infoczas7nfbzac5mhjnxpkxle 377 28 . . . work_infoczas7nfbzac5mhjnxpkxle 378 1 Nucleic Nucleic NNP work_infoczas7nfbzac5mhjnxpkxle 378 2 Acids Acids NNPS work_infoczas7nfbzac5mhjnxpkxle 378 3 Res res VBP work_infoczas7nfbzac5mhjnxpkxle 378 4 25:449 25:449 CD work_infoczas7nfbzac5mhjnxpkxle 378 5 - - SYM work_infoczas7nfbzac5mhjnxpkxle 378 6 450 450 CD work_infoczas7nfbzac5mhjnxpkxle 378 7 . . . work_infoczas7nfbzac5mhjnxpkxle 379 1 Liu Liu NNP work_infoczas7nfbzac5mhjnxpkxle 379 2 G G NNP work_infoczas7nfbzac5mhjnxpkxle 379 3 , , , work_infoczas7nfbzac5mhjnxpkxle 379 4 Geurts Geurts NNP work_infoczas7nfbzac5mhjnxpkxle 379 5 AM am NN work_infoczas7nfbzac5mhjnxpkxle 379 6 , , , work_infoczas7nfbzac5mhjnxpkxle 379 7 Yae Yae NNP work_infoczas7nfbzac5mhjnxpkxle 379 8 K K NNP work_infoczas7nfbzac5mhjnxpkxle 379 9 , , , work_infoczas7nfbzac5mhjnxpkxle 379 10 Srinivasan Srinivasan NNP work_infoczas7nfbzac5mhjnxpkxle 379 11 AR AR NNP work_infoczas7nfbzac5mhjnxpkxle 379 12 , , , work_infoczas7nfbzac5mhjnxpkxle 379 13 Fahrenkrug Fahrenkrug NNP work_infoczas7nfbzac5mhjnxpkxle 379 14 SC SC NNP work_infoczas7nfbzac5mhjnxpkxle 379 15 , , , work_infoczas7nfbzac5mhjnxpkxle 379 16 Largaespada Largaespada NNP work_infoczas7nfbzac5mhjnxpkxle 379 17 DA DA NNP work_infoczas7nfbzac5mhjnxpkxle 379 18 , , , work_infoczas7nfbzac5mhjnxpkxle 379 19 Takeda Takeda NNP work_infoczas7nfbzac5mhjnxpkxle 379 20 J J NNP work_infoczas7nfbzac5mhjnxpkxle 379 21 , , , work_infoczas7nfbzac5mhjnxpkxle 379 22 Horie Horie NNP work_infoczas7nfbzac5mhjnxpkxle 379 23 K K NNP work_infoczas7nfbzac5mhjnxpkxle 379 24 , , , work_infoczas7nfbzac5mhjnxpkxle 379 25 Olson Olson NNP work_infoczas7nfbzac5mhjnxpkxle 379 26 WK WK NNP work_infoczas7nfbzac5mhjnxpkxle 379 27 , , , work_infoczas7nfbzac5mhjnxpkxle 379 28 Hackett Hackett NNP work_infoczas7nfbzac5mhjnxpkxle 379 29 PB PB NNP work_infoczas7nfbzac5mhjnxpkxle 379 30 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 379 31 2005 2005 CD work_infoczas7nfbzac5mhjnxpkxle 379 32 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 379 33 Target target NN work_infoczas7nfbzac5mhjnxpkxle 379 34 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 379 35 site site NN work_infoczas7nfbzac5mhjnxpkxle 379 36 preferences preference NNS work_infoczas7nfbzac5mhjnxpkxle 379 37 of of IN work_infoczas7nfbzac5mhjnxpkxle 379 38 Sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 379 39 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 379 40 transposons transposon NNS work_infoczas7nfbzac5mhjnxpkxle 379 41 . . . work_infoczas7nfbzac5mhjnxpkxle 380 1 J J NNP work_infoczas7nfbzac5mhjnxpkxle 380 2 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 380 3 Biol Biol NNP work_infoczas7nfbzac5mhjnxpkxle 380 4 346:161- 346:161- CD work_infoczas7nfbzac5mhjnxpkxle 380 5 173 173 CD work_infoczas7nfbzac5mhjnxpkxle 380 6 . . . work_infoczas7nfbzac5mhjnxpkxle 381 1 25 25 CD work_infoczas7nfbzac5mhjnxpkxle 381 2 Liu Liu NNP work_infoczas7nfbzac5mhjnxpkxle 381 3 L L NNP work_infoczas7nfbzac5mhjnxpkxle 381 4 , , , work_infoczas7nfbzac5mhjnxpkxle 381 5 Mah Mah NNP work_infoczas7nfbzac5mhjnxpkxle 381 6 C C NNP work_infoczas7nfbzac5mhjnxpkxle 381 7 , , , work_infoczas7nfbzac5mhjnxpkxle 381 8 Fletcher Fletcher NNP work_infoczas7nfbzac5mhjnxpkxle 381 9 BS BS NNP work_infoczas7nfbzac5mhjnxpkxle 381 10 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 381 11 2006 2006 CD work_infoczas7nfbzac5mhjnxpkxle 381 12 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 381 13 Sustained Sustained NNP work_infoczas7nfbzac5mhjnxpkxle 381 14 FVIII FVIII NNP work_infoczas7nfbzac5mhjnxpkxle 381 15 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 381 16 and and CC work_infoczas7nfbzac5mhjnxpkxle 381 17 phenotypic phenotypic NN work_infoczas7nfbzac5mhjnxpkxle 381 18 correction correction NN work_infoczas7nfbzac5mhjnxpkxle 381 19 of of IN work_infoczas7nfbzac5mhjnxpkxle 381 20 hemophilia hemophilia NNP work_infoczas7nfbzac5mhjnxpkxle 381 21 A A NNP work_infoczas7nfbzac5mhjnxpkxle 381 22 in in IN work_infoczas7nfbzac5mhjnxpkxle 381 23 neonatal neonatal JJ work_infoczas7nfbzac5mhjnxpkxle 381 24 mice mouse NNS work_infoczas7nfbzac5mhjnxpkxle 381 25 using use VBG work_infoczas7nfbzac5mhjnxpkxle 381 26 an an DT work_infoczas7nfbzac5mhjnxpkxle 381 27 endothelial endothelial RB work_infoczas7nfbzac5mhjnxpkxle 381 28 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 381 29 targeted target VBN work_infoczas7nfbzac5mhjnxpkxle 381 30 sleeping sleeping NN work_infoczas7nfbzac5mhjnxpkxle 381 31 beauty beauty NN work_infoczas7nfbzac5mhjnxpkxle 381 32 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 381 33 . . . work_infoczas7nfbzac5mhjnxpkxle 382 1 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 382 2 Ther Ther NNP work_infoczas7nfbzac5mhjnxpkxle 382 3 13:1006- 13:1006- NNP work_infoczas7nfbzac5mhjnxpkxle 382 4 1015 1015 CD work_infoczas7nfbzac5mhjnxpkxle 382 5 . . . work_infoczas7nfbzac5mhjnxpkxle 383 1 Mates Mates NNP work_infoczas7nfbzac5mhjnxpkxle 383 2 L L NNP work_infoczas7nfbzac5mhjnxpkxle 383 3 , , , work_infoczas7nfbzac5mhjnxpkxle 383 4 Chuah Chuah NNP work_infoczas7nfbzac5mhjnxpkxle 383 5 MK MK NNP work_infoczas7nfbzac5mhjnxpkxle 383 6 , , , work_infoczas7nfbzac5mhjnxpkxle 383 7 Belay belay NN work_infoczas7nfbzac5mhjnxpkxle 383 8 E E NNS work_infoczas7nfbzac5mhjnxpkxle 383 9 , , , work_infoczas7nfbzac5mhjnxpkxle 383 10 Jerchow Jerchow NNP work_infoczas7nfbzac5mhjnxpkxle 383 11 B B NNP work_infoczas7nfbzac5mhjnxpkxle 383 12 , , , work_infoczas7nfbzac5mhjnxpkxle 383 13 Manoj Manoj NNP work_infoczas7nfbzac5mhjnxpkxle 383 14 N N NNP work_infoczas7nfbzac5mhjnxpkxle 383 15 , , , work_infoczas7nfbzac5mhjnxpkxle 383 16 Acosta Acosta NNP work_infoczas7nfbzac5mhjnxpkxle 383 17 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 383 18 Sanchez Sanchez NNP work_infoczas7nfbzac5mhjnxpkxle 383 19 A A NNP work_infoczas7nfbzac5mhjnxpkxle 383 20 , , , work_infoczas7nfbzac5mhjnxpkxle 383 21 Grzela Grzela NNP work_infoczas7nfbzac5mhjnxpkxle 383 22 DP DP NNP work_infoczas7nfbzac5mhjnxpkxle 383 23 , , , work_infoczas7nfbzac5mhjnxpkxle 383 24 Schmitt Schmitt NNP work_infoczas7nfbzac5mhjnxpkxle 383 25 A A NNP work_infoczas7nfbzac5mhjnxpkxle 383 26 , , , work_infoczas7nfbzac5mhjnxpkxle 383 27 Becker Becker NNP work_infoczas7nfbzac5mhjnxpkxle 383 28 K K NNP work_infoczas7nfbzac5mhjnxpkxle 383 29 , , , work_infoczas7nfbzac5mhjnxpkxle 383 30 Matrai Matrai NNP work_infoczas7nfbzac5mhjnxpkxle 383 31 J J NNP work_infoczas7nfbzac5mhjnxpkxle 383 32 , , , work_infoczas7nfbzac5mhjnxpkxle 383 33 Ma Ma NNP work_infoczas7nfbzac5mhjnxpkxle 383 34 L L NNP work_infoczas7nfbzac5mhjnxpkxle 383 35 , , , work_infoczas7nfbzac5mhjnxpkxle 383 36 Samara Samara NNP work_infoczas7nfbzac5mhjnxpkxle 383 37 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 383 38 Kuko Kuko NNP work_infoczas7nfbzac5mhjnxpkxle 383 39 E e NN work_infoczas7nfbzac5mhjnxpkxle 383 40 , , , work_infoczas7nfbzac5mhjnxpkxle 383 41 Gysemans Gysemans NNP work_infoczas7nfbzac5mhjnxpkxle 383 42 C C NNP work_infoczas7nfbzac5mhjnxpkxle 383 43 , , , work_infoczas7nfbzac5mhjnxpkxle 383 44 Pryputniewicz Pryputniewicz NNP work_infoczas7nfbzac5mhjnxpkxle 383 45 D D NNP work_infoczas7nfbzac5mhjnxpkxle 383 46 , , , work_infoczas7nfbzac5mhjnxpkxle 383 47 Miskey Miskey NNP work_infoczas7nfbzac5mhjnxpkxle 383 48 C C NNP work_infoczas7nfbzac5mhjnxpkxle 383 49 , , , work_infoczas7nfbzac5mhjnxpkxle 383 50 Fletcher Fletcher NNP work_infoczas7nfbzac5mhjnxpkxle 383 51 B B NNP work_infoczas7nfbzac5mhjnxpkxle 383 52 , , , work_infoczas7nfbzac5mhjnxpkxle 383 53 Vandendriessche Vandendriessche NNP work_infoczas7nfbzac5mhjnxpkxle 383 54 T T NNP work_infoczas7nfbzac5mhjnxpkxle 383 55 , , , work_infoczas7nfbzac5mhjnxpkxle 383 56 Ivics Ivics NNP work_infoczas7nfbzac5mhjnxpkxle 383 57 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 383 58 , , , work_infoczas7nfbzac5mhjnxpkxle 383 59 Izsvak Izsvak NNP work_infoczas7nfbzac5mhjnxpkxle 383 60 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 383 61 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 383 62 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 383 63 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 383 64 Molecular molecular JJ work_infoczas7nfbzac5mhjnxpkxle 383 65 evolution evolution NN work_infoczas7nfbzac5mhjnxpkxle 383 66 of of IN work_infoczas7nfbzac5mhjnxpkxle 383 67 a a DT work_infoczas7nfbzac5mhjnxpkxle 383 68 novel novel JJ work_infoczas7nfbzac5mhjnxpkxle 383 69 hyperactive hyperactive JJ work_infoczas7nfbzac5mhjnxpkxle 383 70 Sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 383 71 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 383 72 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 383 73 enables enable VBZ work_infoczas7nfbzac5mhjnxpkxle 383 74 robust robust JJ work_infoczas7nfbzac5mhjnxpkxle 383 75 stable stable JJ work_infoczas7nfbzac5mhjnxpkxle 383 76 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 383 77 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 383 78 in in IN work_infoczas7nfbzac5mhjnxpkxle 383 79 vertebrates vertebrate NNS work_infoczas7nfbzac5mhjnxpkxle 383 80 . . . work_infoczas7nfbzac5mhjnxpkxle 384 1 Nat Nat NNP work_infoczas7nfbzac5mhjnxpkxle 384 2 Genet Genet NNP work_infoczas7nfbzac5mhjnxpkxle 384 3 41:753 41:753 NNS work_infoczas7nfbzac5mhjnxpkxle 384 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 384 5 761 761 CD work_infoczas7nfbzac5mhjnxpkxle 384 6 . . . work_infoczas7nfbzac5mhjnxpkxle 385 1 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 385 2 JG JG NNP work_infoczas7nfbzac5mhjnxpkxle 385 3 , , , work_infoczas7nfbzac5mhjnxpkxle 385 4 Yant Yant NNP work_infoczas7nfbzac5mhjnxpkxle 385 5 SR SR NNP work_infoczas7nfbzac5mhjnxpkxle 385 6 , , , work_infoczas7nfbzac5mhjnxpkxle 385 7 Meuse Meuse NNP work_infoczas7nfbzac5mhjnxpkxle 385 8 L L NNP work_infoczas7nfbzac5mhjnxpkxle 385 9 , , , work_infoczas7nfbzac5mhjnxpkxle 385 10 Huang Huang NNP work_infoczas7nfbzac5mhjnxpkxle 385 11 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 385 12 , , , work_infoczas7nfbzac5mhjnxpkxle 385 13 Xu Xu NNP work_infoczas7nfbzac5mhjnxpkxle 385 14 H H NNP work_infoczas7nfbzac5mhjnxpkxle 385 15 , , , work_infoczas7nfbzac5mhjnxpkxle 385 16 Kay Kay NNP work_infoczas7nfbzac5mhjnxpkxle 385 17 MA MA NNP work_infoczas7nfbzac5mhjnxpkxle 385 18 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 385 19 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 385 20 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 385 21 Helper helper NN work_infoczas7nfbzac5mhjnxpkxle 385 22 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 385 23 Independent Independent NNP work_infoczas7nfbzac5mhjnxpkxle 385 24 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 385 25 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 385 26 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 385 27 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 385 28 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 385 29 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 385 30 for for IN work_infoczas7nfbzac5mhjnxpkxle 385 31 efficient efficient JJ work_infoczas7nfbzac5mhjnxpkxle 385 32 nonviral nonviral JJ work_infoczas7nfbzac5mhjnxpkxle 385 33 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 385 34 delivery delivery NN work_infoczas7nfbzac5mhjnxpkxle 385 35 and and CC work_infoczas7nfbzac5mhjnxpkxle 385 36 persistent persistent JJ work_infoczas7nfbzac5mhjnxpkxle 385 37 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 385 38 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 385 39 in in IN work_infoczas7nfbzac5mhjnxpkxle 385 40 vivo vivo NNP work_infoczas7nfbzac5mhjnxpkxle 385 41 . . . work_infoczas7nfbzac5mhjnxpkxle 386 1 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 386 2 Ther Ther NNP work_infoczas7nfbzac5mhjnxpkxle 386 3 8:654 8:654 NNP work_infoczas7nfbzac5mhjnxpkxle 386 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 386 5 665 665 CD work_infoczas7nfbzac5mhjnxpkxle 386 6 . . . work_infoczas7nfbzac5mhjnxpkxle 387 1 Mitchell Mitchell NNP work_infoczas7nfbzac5mhjnxpkxle 387 2 RS RS NNP work_infoczas7nfbzac5mhjnxpkxle 387 3 , , , work_infoczas7nfbzac5mhjnxpkxle 387 4 Beitzel Beitzel NNP work_infoczas7nfbzac5mhjnxpkxle 387 5 BF BF NNP work_infoczas7nfbzac5mhjnxpkxle 387 6 , , , work_infoczas7nfbzac5mhjnxpkxle 387 7 Schroder Schroder NNP work_infoczas7nfbzac5mhjnxpkxle 387 8 AR AR NNP work_infoczas7nfbzac5mhjnxpkxle 387 9 , , , work_infoczas7nfbzac5mhjnxpkxle 387 10 Shinn Shinn NNP work_infoczas7nfbzac5mhjnxpkxle 387 11 P P NNP work_infoczas7nfbzac5mhjnxpkxle 387 12 , , , work_infoczas7nfbzac5mhjnxpkxle 387 13 Chen Chen NNP work_infoczas7nfbzac5mhjnxpkxle 387 14 H H NNP work_infoczas7nfbzac5mhjnxpkxle 387 15 , , , work_infoczas7nfbzac5mhjnxpkxle 387 16 Berry Berry NNP work_infoczas7nfbzac5mhjnxpkxle 387 17 CC CC NNP work_infoczas7nfbzac5mhjnxpkxle 387 18 , , , work_infoczas7nfbzac5mhjnxpkxle 387 19 Ecker Ecker NNP work_infoczas7nfbzac5mhjnxpkxle 387 20 JR JR NNP work_infoczas7nfbzac5mhjnxpkxle 387 21 , , , work_infoczas7nfbzac5mhjnxpkxle 387 22 Bushman Bushman NNP work_infoczas7nfbzac5mhjnxpkxle 387 23 FD FD NNP work_infoczas7nfbzac5mhjnxpkxle 387 24 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 387 25 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 387 26 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 387 27 Retroviral retroviral JJ work_infoczas7nfbzac5mhjnxpkxle 387 28 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 387 29 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 387 30 : : : work_infoczas7nfbzac5mhjnxpkxle 387 31 ASLV aslv NN work_infoczas7nfbzac5mhjnxpkxle 387 32 , , , work_infoczas7nfbzac5mhjnxpkxle 387 33 HIV HIV NNP work_infoczas7nfbzac5mhjnxpkxle 387 34 , , , work_infoczas7nfbzac5mhjnxpkxle 387 35 and and CC work_infoczas7nfbzac5mhjnxpkxle 387 36 MLV MLV NNP work_infoczas7nfbzac5mhjnxpkxle 387 37 show show VBP work_infoczas7nfbzac5mhjnxpkxle 387 38 distinct distinct JJ work_infoczas7nfbzac5mhjnxpkxle 387 39 target target NN work_infoczas7nfbzac5mhjnxpkxle 387 40 site site NN work_infoczas7nfbzac5mhjnxpkxle 387 41 preferences preference NNS work_infoczas7nfbzac5mhjnxpkxle 387 42 . . . work_infoczas7nfbzac5mhjnxpkxle 388 1 PLoS PLoS NNP work_infoczas7nfbzac5mhjnxpkxle 388 2 Biol Biol NNP work_infoczas7nfbzac5mhjnxpkxle 388 3 2 2 CD work_infoczas7nfbzac5mhjnxpkxle 388 4 : : : work_infoczas7nfbzac5mhjnxpkxle 388 5 E234 e234 PRP work_infoczas7nfbzac5mhjnxpkxle 388 6 . . . work_infoczas7nfbzac5mhjnxpkxle 389 1 Moldt Moldt NNP work_infoczas7nfbzac5mhjnxpkxle 389 2 B B NNP work_infoczas7nfbzac5mhjnxpkxle 389 3 , , , work_infoczas7nfbzac5mhjnxpkxle 389 4 Staunstrup Staunstrup NNP work_infoczas7nfbzac5mhjnxpkxle 389 5 NH NH NNP work_infoczas7nfbzac5mhjnxpkxle 389 6 , , , work_infoczas7nfbzac5mhjnxpkxle 389 7 Jakobsen Jakobsen NNP work_infoczas7nfbzac5mhjnxpkxle 389 8 M M NNP work_infoczas7nfbzac5mhjnxpkxle 389 9 , , , work_infoczas7nfbzac5mhjnxpkxle 389 10 Yanez Yanez NNP work_infoczas7nfbzac5mhjnxpkxle 389 11 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 389 12 Munoz Munoz NNP work_infoczas7nfbzac5mhjnxpkxle 389 13 RJ RJ NNP work_infoczas7nfbzac5mhjnxpkxle 389 14 , , , work_infoczas7nfbzac5mhjnxpkxle 389 15 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 389 16 JG JG NNP work_infoczas7nfbzac5mhjnxpkxle 389 17 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 389 18 2008 2008 CD work_infoczas7nfbzac5mhjnxpkxle 389 19 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 389 20 Genomic genomic JJ work_infoczas7nfbzac5mhjnxpkxle 389 21 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 389 22 of of IN work_infoczas7nfbzac5mhjnxpkxle 389 23 lentiviral lentiviral JJ work_infoczas7nfbzac5mhjnxpkxle 389 24 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 389 25 circles circle NNS work_infoczas7nfbzac5mhjnxpkxle 389 26 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 389 27 by by IN work_infoczas7nfbzac5mhjnxpkxle 389 28 the the DT work_infoczas7nfbzac5mhjnxpkxle 389 29 yeast yeast NN work_infoczas7nfbzac5mhjnxpkxle 389 30 Flp flp NN work_infoczas7nfbzac5mhjnxpkxle 389 31 recombinase recombinase NN work_infoczas7nfbzac5mhjnxpkxle 389 32 . . . work_infoczas7nfbzac5mhjnxpkxle 390 1 BMC BMC NNP work_infoczas7nfbzac5mhjnxpkxle 390 2 Biotechnol Biotechnol NNP work_infoczas7nfbzac5mhjnxpkxle 390 3 8:60 8:60 CD work_infoczas7nfbzac5mhjnxpkxle 390 4 . . . work_infoczas7nfbzac5mhjnxpkxle 391 1 Naruse Naruse NNP work_infoczas7nfbzac5mhjnxpkxle 391 2 K K NNP work_infoczas7nfbzac5mhjnxpkxle 391 3 , , , work_infoczas7nfbzac5mhjnxpkxle 391 4 Ishikawa Ishikawa NNP work_infoczas7nfbzac5mhjnxpkxle 391 5 H H NNP work_infoczas7nfbzac5mhjnxpkxle 391 6 , , , work_infoczas7nfbzac5mhjnxpkxle 391 7 Kawano Kawano NNP work_infoczas7nfbzac5mhjnxpkxle 391 8 HO HO NNP work_infoczas7nfbzac5mhjnxpkxle 391 9 , , , work_infoczas7nfbzac5mhjnxpkxle 391 10 Ueda Ueda NNP work_infoczas7nfbzac5mhjnxpkxle 391 11 H H NNP work_infoczas7nfbzac5mhjnxpkxle 391 12 , , , work_infoczas7nfbzac5mhjnxpkxle 391 13 Kurome Kurome NNP work_infoczas7nfbzac5mhjnxpkxle 391 14 M M NNP work_infoczas7nfbzac5mhjnxpkxle 391 15 , , , work_infoczas7nfbzac5mhjnxpkxle 391 16 Miyazaki Miyazaki NNP work_infoczas7nfbzac5mhjnxpkxle 391 17 K K NNP work_infoczas7nfbzac5mhjnxpkxle 391 18 , , , work_infoczas7nfbzac5mhjnxpkxle 391 19 Endo Endo NNP work_infoczas7nfbzac5mhjnxpkxle 391 20 M M NNP work_infoczas7nfbzac5mhjnxpkxle 391 21 , , , work_infoczas7nfbzac5mhjnxpkxle 391 22 Sawasaki Sawasaki NNP work_infoczas7nfbzac5mhjnxpkxle 391 23 T T NNP work_infoczas7nfbzac5mhjnxpkxle 391 24 , , , work_infoczas7nfbzac5mhjnxpkxle 391 25 Nagashima Nagashima NNP work_infoczas7nfbzac5mhjnxpkxle 391 26 H H NNP work_infoczas7nfbzac5mhjnxpkxle 391 27 , , , work_infoczas7nfbzac5mhjnxpkxle 391 28 Makuuchi Makuuchi NNP work_infoczas7nfbzac5mhjnxpkxle 391 29 M M NNP work_infoczas7nfbzac5mhjnxpkxle 391 30 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 391 31 2005 2005 CD work_infoczas7nfbzac5mhjnxpkxle 391 32 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 391 33 Production production NN work_infoczas7nfbzac5mhjnxpkxle 391 34 of of IN work_infoczas7nfbzac5mhjnxpkxle 391 35 a a DT work_infoczas7nfbzac5mhjnxpkxle 391 36 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 391 37 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 391 38 expressing express VBG work_infoczas7nfbzac5mhjnxpkxle 391 39 human human JJ work_infoczas7nfbzac5mhjnxpkxle 391 40 albumin albumin NN work_infoczas7nfbzac5mhjnxpkxle 391 41 and and CC work_infoczas7nfbzac5mhjnxpkxle 391 42 enhanced enhance VBD work_infoczas7nfbzac5mhjnxpkxle 391 43 green green JJ work_infoczas7nfbzac5mhjnxpkxle 391 44 fluorescent fluorescent NN work_infoczas7nfbzac5mhjnxpkxle 391 45 protein protein NN work_infoczas7nfbzac5mhjnxpkxle 391 46 . . . work_infoczas7nfbzac5mhjnxpkxle 392 1 J J NNP work_infoczas7nfbzac5mhjnxpkxle 392 2 Reprod Reprod NNP work_infoczas7nfbzac5mhjnxpkxle 392 3 Dev Dev NNP work_infoczas7nfbzac5mhjnxpkxle 392 4 51:539 51:539 CD work_infoczas7nfbzac5mhjnxpkxle 392 5 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 392 6 546 546 CD work_infoczas7nfbzac5mhjnxpkxle 392 7 . . . work_infoczas7nfbzac5mhjnxpkxle 393 1 Nottle Nottle NNP work_infoczas7nfbzac5mhjnxpkxle 393 2 MB MB NNP work_infoczas7nfbzac5mhjnxpkxle 393 3 , , , work_infoczas7nfbzac5mhjnxpkxle 393 4 Haskard Haskard NNP work_infoczas7nfbzac5mhjnxpkxle 393 5 KA KA NNP work_infoczas7nfbzac5mhjnxpkxle 393 6 , , , work_infoczas7nfbzac5mhjnxpkxle 393 7 Verma Verma NNP work_infoczas7nfbzac5mhjnxpkxle 393 8 PJ PJ NNP work_infoczas7nfbzac5mhjnxpkxle 393 9 , , , work_infoczas7nfbzac5mhjnxpkxle 393 10 Du Du NNP work_infoczas7nfbzac5mhjnxpkxle 393 11 ZT ZT NNP work_infoczas7nfbzac5mhjnxpkxle 393 12 , , , work_infoczas7nfbzac5mhjnxpkxle 393 13 Grupen Grupen NNP work_infoczas7nfbzac5mhjnxpkxle 393 14 CG CG NNP work_infoczas7nfbzac5mhjnxpkxle 393 15 , , , work_infoczas7nfbzac5mhjnxpkxle 393 16 McIlfatrick McIlfatrick NNP work_infoczas7nfbzac5mhjnxpkxle 393 17 SM SM NNP work_infoczas7nfbzac5mhjnxpkxle 393 18 , , , work_infoczas7nfbzac5mhjnxpkxle 393 19 Ashman Ashman NNP work_infoczas7nfbzac5mhjnxpkxle 393 20 RJ RJ NNP work_infoczas7nfbzac5mhjnxpkxle 393 21 , , , work_infoczas7nfbzac5mhjnxpkxle 393 22 Harrison Harrison NNP work_infoczas7nfbzac5mhjnxpkxle 393 23 SJ SJ NNP work_infoczas7nfbzac5mhjnxpkxle 393 24 , , , work_infoczas7nfbzac5mhjnxpkxle 393 25 Barlow Barlow NNP work_infoczas7nfbzac5mhjnxpkxle 393 26 H H NNP work_infoczas7nfbzac5mhjnxpkxle 393 27 , , , work_infoczas7nfbzac5mhjnxpkxle 393 28 Wigley Wigley NNP work_infoczas7nfbzac5mhjnxpkxle 393 29 PL PL NNP work_infoczas7nfbzac5mhjnxpkxle 393 30 , , , work_infoczas7nfbzac5mhjnxpkxle 393 31 Lyons Lyons NNP work_infoczas7nfbzac5mhjnxpkxle 393 32 IG IG NNP work_infoczas7nfbzac5mhjnxpkxle 393 33 , , , work_infoczas7nfbzac5mhjnxpkxle 393 34 Cowan Cowan NNP work_infoczas7nfbzac5mhjnxpkxle 393 35 PJ PJ NNP work_infoczas7nfbzac5mhjnxpkxle 393 36 , , , work_infoczas7nfbzac5mhjnxpkxle 393 37 Crawford Crawford NNP work_infoczas7nfbzac5mhjnxpkxle 393 38 RJ RJ NNP work_infoczas7nfbzac5mhjnxpkxle 393 39 , , , work_infoczas7nfbzac5mhjnxpkxle 393 40 Tolstoshev Tolstoshev NNP work_infoczas7nfbzac5mhjnxpkxle 393 41 PL PL NNP work_infoczas7nfbzac5mhjnxpkxle 393 42 , , , work_infoczas7nfbzac5mhjnxpkxle 393 43 Pearse Pearse NNP work_infoczas7nfbzac5mhjnxpkxle 393 44 MJ MJ NNP work_infoczas7nfbzac5mhjnxpkxle 393 45 , , , work_infoczas7nfbzac5mhjnxpkxle 393 46 Robins Robins NNP work_infoczas7nfbzac5mhjnxpkxle 393 47 AJ AJ NNP work_infoczas7nfbzac5mhjnxpkxle 393 48 , , , work_infoczas7nfbzac5mhjnxpkxle 393 49 d'Apice d'Apice NNP work_infoczas7nfbzac5mhjnxpkxle 393 50 AJ AJ NNP work_infoczas7nfbzac5mhjnxpkxle 393 51 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 393 52 2001 2001 CD work_infoczas7nfbzac5mhjnxpkxle 393 53 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 393 54 Effect effect NN work_infoczas7nfbzac5mhjnxpkxle 393 55 of of IN work_infoczas7nfbzac5mhjnxpkxle 393 56 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 393 57 concentration concentration NN work_infoczas7nfbzac5mhjnxpkxle 393 58 on on IN work_infoczas7nfbzac5mhjnxpkxle 393 59 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 393 60 rates rate NNS work_infoczas7nfbzac5mhjnxpkxle 393 61 in in IN work_infoczas7nfbzac5mhjnxpkxle 393 62 mice mouse NNS work_infoczas7nfbzac5mhjnxpkxle 393 63 and and CC work_infoczas7nfbzac5mhjnxpkxle 393 64 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 393 65 . . . work_infoczas7nfbzac5mhjnxpkxle 394 1 Transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 394 2 Res Res NNP work_infoczas7nfbzac5mhjnxpkxle 394 3 10:523 10:523 NNS work_infoczas7nfbzac5mhjnxpkxle 394 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 394 5 531 531 CD work_infoczas7nfbzac5mhjnxpkxle 394 6 . . . work_infoczas7nfbzac5mhjnxpkxle 395 1 Ohlfest Ohlfest NNP work_infoczas7nfbzac5mhjnxpkxle 395 2 JR JR NNP work_infoczas7nfbzac5mhjnxpkxle 395 3 , , , work_infoczas7nfbzac5mhjnxpkxle 395 4 Lobitz Lobitz NNP work_infoczas7nfbzac5mhjnxpkxle 395 5 PD PD NNP work_infoczas7nfbzac5mhjnxpkxle 395 6 , , , work_infoczas7nfbzac5mhjnxpkxle 395 7 Perkinson Perkinson NNP work_infoczas7nfbzac5mhjnxpkxle 395 8 SG SG NNP work_infoczas7nfbzac5mhjnxpkxle 395 9 , , , work_infoczas7nfbzac5mhjnxpkxle 395 10 Largaespada Largaespada NNP work_infoczas7nfbzac5mhjnxpkxle 395 11 DA DA NNP work_infoczas7nfbzac5mhjnxpkxle 395 12 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 395 13 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 395 14 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 395 15 Integration integration NN work_infoczas7nfbzac5mhjnxpkxle 395 16 and and CC work_infoczas7nfbzac5mhjnxpkxle 395 17 long long JJ work_infoczas7nfbzac5mhjnxpkxle 395 18 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 395 19 term term NN work_infoczas7nfbzac5mhjnxpkxle 395 20 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 395 21 in in IN work_infoczas7nfbzac5mhjnxpkxle 395 22 xenografted xenografted NNP work_infoczas7nfbzac5mhjnxpkxle 395 23 human human JJ work_infoczas7nfbzac5mhjnxpkxle 395 24 glioblastoma glioblastoma NN work_infoczas7nfbzac5mhjnxpkxle 395 25 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 395 26 using use VBG work_infoczas7nfbzac5mhjnxpkxle 395 27 a a DT work_infoczas7nfbzac5mhjnxpkxle 395 28 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 395 29 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 395 30 based base VBN work_infoczas7nfbzac5mhjnxpkxle 395 31 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 395 32 system system NN work_infoczas7nfbzac5mhjnxpkxle 395 33 . . . work_infoczas7nfbzac5mhjnxpkxle 396 1 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 396 2 Ther Ther NNP work_infoczas7nfbzac5mhjnxpkxle 396 3 10:260 10:260 CD work_infoczas7nfbzac5mhjnxpkxle 396 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 396 5 268 268 CD work_infoczas7nfbzac5mhjnxpkxle 396 6 . . . work_infoczas7nfbzac5mhjnxpkxle 397 1 26 26 CD work_infoczas7nfbzac5mhjnxpkxle 397 2 Onishi Onishi NNP work_infoczas7nfbzac5mhjnxpkxle 397 3 A A NNP work_infoczas7nfbzac5mhjnxpkxle 397 4 , , , work_infoczas7nfbzac5mhjnxpkxle 397 5 Iwamoto Iwamoto NNP work_infoczas7nfbzac5mhjnxpkxle 397 6 M M NNP work_infoczas7nfbzac5mhjnxpkxle 397 7 , , , work_infoczas7nfbzac5mhjnxpkxle 397 8 Akita Akita NNP work_infoczas7nfbzac5mhjnxpkxle 397 9 T T NNP work_infoczas7nfbzac5mhjnxpkxle 397 10 , , , work_infoczas7nfbzac5mhjnxpkxle 397 11 Mikawa Mikawa NNP work_infoczas7nfbzac5mhjnxpkxle 397 12 S S NNP work_infoczas7nfbzac5mhjnxpkxle 397 13 , , , work_infoczas7nfbzac5mhjnxpkxle 397 14 Takeda Takeda NNP work_infoczas7nfbzac5mhjnxpkxle 397 15 K K NNP work_infoczas7nfbzac5mhjnxpkxle 397 16 , , , work_infoczas7nfbzac5mhjnxpkxle 397 17 Awata Awata NNP work_infoczas7nfbzac5mhjnxpkxle 397 18 T T NNP work_infoczas7nfbzac5mhjnxpkxle 397 19 , , , work_infoczas7nfbzac5mhjnxpkxle 397 20 Hanada Hanada NNP work_infoczas7nfbzac5mhjnxpkxle 397 21 H H NNP work_infoczas7nfbzac5mhjnxpkxle 397 22 , , , work_infoczas7nfbzac5mhjnxpkxle 397 23 Perry Perry NNP work_infoczas7nfbzac5mhjnxpkxle 397 24 AC AC NNP work_infoczas7nfbzac5mhjnxpkxle 397 25 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 397 26 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 397 27 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 397 28 Pig pig NN work_infoczas7nfbzac5mhjnxpkxle 397 29 cloning clone VBG work_infoczas7nfbzac5mhjnxpkxle 397 30 by by IN work_infoczas7nfbzac5mhjnxpkxle 397 31 microinjection microinjection NN work_infoczas7nfbzac5mhjnxpkxle 397 32 of of IN work_infoczas7nfbzac5mhjnxpkxle 397 33 fetal fetal JJ work_infoczas7nfbzac5mhjnxpkxle 397 34 fibroblast fibroblast NN work_infoczas7nfbzac5mhjnxpkxle 397 35 nuclei nucleus NNS work_infoczas7nfbzac5mhjnxpkxle 397 36 . . . work_infoczas7nfbzac5mhjnxpkxle 398 1 Science Science NNP work_infoczas7nfbzac5mhjnxpkxle 398 2 289:1188 289:1188 NNP work_infoczas7nfbzac5mhjnxpkxle 398 3 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 398 4 1190 1190 CD work_infoczas7nfbzac5mhjnxpkxle 398 5 . . . work_infoczas7nfbzac5mhjnxpkxle 399 1 Park Park NNP work_infoczas7nfbzac5mhjnxpkxle 399 2 KW KW NNP work_infoczas7nfbzac5mhjnxpkxle 399 3 , , , work_infoczas7nfbzac5mhjnxpkxle 399 4 Cheong Cheong NNP work_infoczas7nfbzac5mhjnxpkxle 399 5 HT HT NNP work_infoczas7nfbzac5mhjnxpkxle 399 6 , , , work_infoczas7nfbzac5mhjnxpkxle 399 7 Lai Lai NNP work_infoczas7nfbzac5mhjnxpkxle 399 8 L L NNP work_infoczas7nfbzac5mhjnxpkxle 399 9 , , , work_infoczas7nfbzac5mhjnxpkxle 399 10 I -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 399 11 m be VBP work_infoczas7nfbzac5mhjnxpkxle 399 12 GS GS NNP work_infoczas7nfbzac5mhjnxpkxle 399 13 , , , work_infoczas7nfbzac5mhjnxpkxle 399 14 Kuhholzer Kuhholzer NNP work_infoczas7nfbzac5mhjnxpkxle 399 15 B B NNP work_infoczas7nfbzac5mhjnxpkxle 399 16 , , , work_infoczas7nfbzac5mhjnxpkxle 399 17 Bonk Bonk NNP work_infoczas7nfbzac5mhjnxpkxle 399 18 A A NNP work_infoczas7nfbzac5mhjnxpkxle 399 19 , , , work_infoczas7nfbzac5mhjnxpkxle 399 20 Samuel Samuel NNP work_infoczas7nfbzac5mhjnxpkxle 399 21 M M NNP work_infoczas7nfbzac5mhjnxpkxle 399 22 , , , work_infoczas7nfbzac5mhjnxpkxle 399 23 Rieke Rieke NNP work_infoczas7nfbzac5mhjnxpkxle 399 24 A A NNP work_infoczas7nfbzac5mhjnxpkxle 399 25 , , , work_infoczas7nfbzac5mhjnxpkxle 399 26 Day Day NNP work_infoczas7nfbzac5mhjnxpkxle 399 27 BN BN NNP work_infoczas7nfbzac5mhjnxpkxle 399 28 , , , work_infoczas7nfbzac5mhjnxpkxle 399 29 Murphy Murphy NNP work_infoczas7nfbzac5mhjnxpkxle 399 30 CN CN NNP work_infoczas7nfbzac5mhjnxpkxle 399 31 , , , work_infoczas7nfbzac5mhjnxpkxle 399 32 Carter Carter NNP work_infoczas7nfbzac5mhjnxpkxle 399 33 DB DB NNP work_infoczas7nfbzac5mhjnxpkxle 399 34 , , , work_infoczas7nfbzac5mhjnxpkxle 399 35 Prather Prather NNP work_infoczas7nfbzac5mhjnxpkxle 399 36 RS RS NNP work_infoczas7nfbzac5mhjnxpkxle 399 37 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 399 38 2001 2001 CD work_infoczas7nfbzac5mhjnxpkxle 399 39 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 399 40 Production production NN work_infoczas7nfbzac5mhjnxpkxle 399 41 of of IN work_infoczas7nfbzac5mhjnxpkxle 399 42 nuclear nuclear JJ work_infoczas7nfbzac5mhjnxpkxle 399 43 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 399 44 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 399 45 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 399 46 swine swine NNS work_infoczas7nfbzac5mhjnxpkxle 399 47 that that WDT work_infoczas7nfbzac5mhjnxpkxle 399 48 express express VBP work_infoczas7nfbzac5mhjnxpkxle 399 49 the the DT work_infoczas7nfbzac5mhjnxpkxle 399 50 enhanced enhanced JJ work_infoczas7nfbzac5mhjnxpkxle 399 51 green green JJ work_infoczas7nfbzac5mhjnxpkxle 399 52 fluorescent fluorescent NN work_infoczas7nfbzac5mhjnxpkxle 399 53 protein protein NN work_infoczas7nfbzac5mhjnxpkxle 399 54 . . . work_infoczas7nfbzac5mhjnxpkxle 400 1 Anim Anim NNP work_infoczas7nfbzac5mhjnxpkxle 400 2 Biotechnol Biotechnol NNP work_infoczas7nfbzac5mhjnxpkxle 400 3 12:173 12:173 CD work_infoczas7nfbzac5mhjnxpkxle 400 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 400 5 181 181 CD work_infoczas7nfbzac5mhjnxpkxle 400 6 . . . work_infoczas7nfbzac5mhjnxpkxle 401 1 Park Park NNP work_infoczas7nfbzac5mhjnxpkxle 401 2 KW KW NNP work_infoczas7nfbzac5mhjnxpkxle 401 3 , , , work_infoczas7nfbzac5mhjnxpkxle 401 4 Lai Lai NNP work_infoczas7nfbzac5mhjnxpkxle 401 5 L L NNP work_infoczas7nfbzac5mhjnxpkxle 401 6 , , , work_infoczas7nfbzac5mhjnxpkxle 401 7 Cheong Cheong NNP work_infoczas7nfbzac5mhjnxpkxle 401 8 HT HT NNP work_infoczas7nfbzac5mhjnxpkxle 401 9 , , , work_infoczas7nfbzac5mhjnxpkxle 401 10 Cabot Cabot NNP work_infoczas7nfbzac5mhjnxpkxle 401 11 R R NNP work_infoczas7nfbzac5mhjnxpkxle 401 12 , , , work_infoczas7nfbzac5mhjnxpkxle 401 13 Sun Sun NNP work_infoczas7nfbzac5mhjnxpkxle 401 14 QY QY NNP work_infoczas7nfbzac5mhjnxpkxle 401 15 , , , work_infoczas7nfbzac5mhjnxpkxle 401 16 Wu Wu NNP work_infoczas7nfbzac5mhjnxpkxle 401 17 G G NNP work_infoczas7nfbzac5mhjnxpkxle 401 18 , , , work_infoczas7nfbzac5mhjnxpkxle 401 19 Rucker Rucker NNP work_infoczas7nfbzac5mhjnxpkxle 401 20 EB EB NNP work_infoczas7nfbzac5mhjnxpkxle 401 21 , , , work_infoczas7nfbzac5mhjnxpkxle 401 22 Durtschi Durtschi NNP work_infoczas7nfbzac5mhjnxpkxle 401 23 D D NNP work_infoczas7nfbzac5mhjnxpkxle 401 24 , , , work_infoczas7nfbzac5mhjnxpkxle 401 25 Bonk Bonk NNP work_infoczas7nfbzac5mhjnxpkxle 401 26 A A NNP work_infoczas7nfbzac5mhjnxpkxle 401 27 , , , work_infoczas7nfbzac5mhjnxpkxle 401 28 Samuel Samuel NNP work_infoczas7nfbzac5mhjnxpkxle 401 29 M M NNP work_infoczas7nfbzac5mhjnxpkxle 401 30 , , , work_infoczas7nfbzac5mhjnxpkxle 401 31 Rieke Rieke NNP work_infoczas7nfbzac5mhjnxpkxle 401 32 A A NNP work_infoczas7nfbzac5mhjnxpkxle 401 33 , , , work_infoczas7nfbzac5mhjnxpkxle 401 34 Day Day NNP work_infoczas7nfbzac5mhjnxpkxle 401 35 BN BN NNP work_infoczas7nfbzac5mhjnxpkxle 401 36 , , , work_infoczas7nfbzac5mhjnxpkxle 401 37 Murphy Murphy NNP work_infoczas7nfbzac5mhjnxpkxle 401 38 CN CN NNP work_infoczas7nfbzac5mhjnxpkxle 401 39 , , , work_infoczas7nfbzac5mhjnxpkxle 401 40 Carter Carter NNP work_infoczas7nfbzac5mhjnxpkxle 401 41 DB DB NNP work_infoczas7nfbzac5mhjnxpkxle 401 42 , , , work_infoczas7nfbzac5mhjnxpkxle 401 43 Prather Prather NNP work_infoczas7nfbzac5mhjnxpkxle 401 44 RS RS NNP work_infoczas7nfbzac5mhjnxpkxle 401 45 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 401 46 2002 2002 CD work_infoczas7nfbzac5mhjnxpkxle 401 47 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 401 48 Mosaic Mosaic NNP work_infoczas7nfbzac5mhjnxpkxle 401 49 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 401 50 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 401 51 in in IN work_infoczas7nfbzac5mhjnxpkxle 401 52 nuclear nuclear JJ work_infoczas7nfbzac5mhjnxpkxle 401 53 transfer- transfer- NN work_infoczas7nfbzac5mhjnxpkxle 401 54 derived derive VBD work_infoczas7nfbzac5mhjnxpkxle 401 55 embryos embryo NNS work_infoczas7nfbzac5mhjnxpkxle 401 56 and and CC work_infoczas7nfbzac5mhjnxpkxle 401 57 the the DT work_infoczas7nfbzac5mhjnxpkxle 401 58 production production NN work_infoczas7nfbzac5mhjnxpkxle 401 59 of of IN work_infoczas7nfbzac5mhjnxpkxle 401 60 cloned clone VBN work_infoczas7nfbzac5mhjnxpkxle 401 61 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 401 62 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 401 63 from from IN work_infoczas7nfbzac5mhjnxpkxle 401 64 ear ear NN work_infoczas7nfbzac5mhjnxpkxle 401 65 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 401 66 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 401 67 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 401 68 . . . work_infoczas7nfbzac5mhjnxpkxle 402 1 Biol Biol NNP work_infoczas7nfbzac5mhjnxpkxle 402 2 Reprod Reprod NNP work_infoczas7nfbzac5mhjnxpkxle 402 3 66:1001 66:1001 SYM work_infoczas7nfbzac5mhjnxpkxle 402 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 402 5 1005 1005 CD work_infoczas7nfbzac5mhjnxpkxle 402 6 . . . work_infoczas7nfbzac5mhjnxpkxle 403 1 Polejaeva Polejaeva NNP work_infoczas7nfbzac5mhjnxpkxle 403 2 IA IA NNP work_infoczas7nfbzac5mhjnxpkxle 403 3 , , , work_infoczas7nfbzac5mhjnxpkxle 403 4 Chen Chen NNP work_infoczas7nfbzac5mhjnxpkxle 403 5 SH SH NNP work_infoczas7nfbzac5mhjnxpkxle 403 6 , , , work_infoczas7nfbzac5mhjnxpkxle 403 7 Vaught Vaught NNP work_infoczas7nfbzac5mhjnxpkxle 403 8 TD TD NNP work_infoczas7nfbzac5mhjnxpkxle 403 9 , , , work_infoczas7nfbzac5mhjnxpkxle 403 10 Page page NN work_infoczas7nfbzac5mhjnxpkxle 403 11 RL RL NNP work_infoczas7nfbzac5mhjnxpkxle 403 12 , , , work_infoczas7nfbzac5mhjnxpkxle 403 13 Mullins Mullins NNP work_infoczas7nfbzac5mhjnxpkxle 403 14 J J NNP work_infoczas7nfbzac5mhjnxpkxle 403 15 , , , work_infoczas7nfbzac5mhjnxpkxle 403 16 Ball Ball NNP work_infoczas7nfbzac5mhjnxpkxle 403 17 S S NNP work_infoczas7nfbzac5mhjnxpkxle 403 18 , , , work_infoczas7nfbzac5mhjnxpkxle 403 19 Dai Dai NNP work_infoczas7nfbzac5mhjnxpkxle 403 20 Y Y NNP work_infoczas7nfbzac5mhjnxpkxle 403 21 , , , work_infoczas7nfbzac5mhjnxpkxle 403 22 Boone Boone NNP work_infoczas7nfbzac5mhjnxpkxle 403 23 J J NNP work_infoczas7nfbzac5mhjnxpkxle 403 24 , , , work_infoczas7nfbzac5mhjnxpkxle 403 25 Walker Walker NNP work_infoczas7nfbzac5mhjnxpkxle 403 26 S S NNP work_infoczas7nfbzac5mhjnxpkxle 403 27 , , , work_infoczas7nfbzac5mhjnxpkxle 403 28 Ayares Ayares NNP work_infoczas7nfbzac5mhjnxpkxle 403 29 DL DL NNP work_infoczas7nfbzac5mhjnxpkxle 403 30 , , , work_infoczas7nfbzac5mhjnxpkxle 403 31 Colman Colman NNP work_infoczas7nfbzac5mhjnxpkxle 403 32 A A NNP work_infoczas7nfbzac5mhjnxpkxle 403 33 , , , work_infoczas7nfbzac5mhjnxpkxle 403 34 Campbell Campbell NNP work_infoczas7nfbzac5mhjnxpkxle 403 35 KH KH NNP work_infoczas7nfbzac5mhjnxpkxle 403 36 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 403 37 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 403 38 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 403 39 Cloned clone VBN work_infoczas7nfbzac5mhjnxpkxle 403 40 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 403 41 produced produce VBN work_infoczas7nfbzac5mhjnxpkxle 403 42 by by IN work_infoczas7nfbzac5mhjnxpkxle 403 43 nuclear nuclear JJ work_infoczas7nfbzac5mhjnxpkxle 403 44 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 403 45 from from IN work_infoczas7nfbzac5mhjnxpkxle 403 46 adult adult JJ work_infoczas7nfbzac5mhjnxpkxle 403 47 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 403 48 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 403 49 . . . work_infoczas7nfbzac5mhjnxpkxle 404 1 Nature nature NN work_infoczas7nfbzac5mhjnxpkxle 404 2 407:86 407:86 CD work_infoczas7nfbzac5mhjnxpkxle 404 3 - - SYM work_infoczas7nfbzac5mhjnxpkxle 404 4 90 90 CD work_infoczas7nfbzac5mhjnxpkxle 404 5 . . . work_infoczas7nfbzac5mhjnxpkxle 405 1 Schorpp Schorpp NNP work_infoczas7nfbzac5mhjnxpkxle 405 2 M M NNP work_infoczas7nfbzac5mhjnxpkxle 405 3 , , , work_infoczas7nfbzac5mhjnxpkxle 405 4 Jager Jager NNP work_infoczas7nfbzac5mhjnxpkxle 405 5 R R NNP work_infoczas7nfbzac5mhjnxpkxle 405 6 , , , work_infoczas7nfbzac5mhjnxpkxle 405 7 Schellander Schellander NNP work_infoczas7nfbzac5mhjnxpkxle 405 8 K K NNP work_infoczas7nfbzac5mhjnxpkxle 405 9 , , , work_infoczas7nfbzac5mhjnxpkxle 405 10 Schenkel Schenkel NNP work_infoczas7nfbzac5mhjnxpkxle 405 11 J J NNP work_infoczas7nfbzac5mhjnxpkxle 405 12 , , , work_infoczas7nfbzac5mhjnxpkxle 405 13 Wagner Wagner NNP work_infoczas7nfbzac5mhjnxpkxle 405 14 EF EF NNP work_infoczas7nfbzac5mhjnxpkxle 405 15 , , , work_infoczas7nfbzac5mhjnxpkxle 405 16 Weiher Weiher NNP work_infoczas7nfbzac5mhjnxpkxle 405 17 H H NNP work_infoczas7nfbzac5mhjnxpkxle 405 18 , , , work_infoczas7nfbzac5mhjnxpkxle 405 19 Angel Angel NNP work_infoczas7nfbzac5mhjnxpkxle 405 20 P P NNP work_infoczas7nfbzac5mhjnxpkxle 405 21 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 405 22 1996 1996 CD work_infoczas7nfbzac5mhjnxpkxle 405 23 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 405 24 The the DT work_infoczas7nfbzac5mhjnxpkxle 405 25 human human NN work_infoczas7nfbzac5mhjnxpkxle 405 26 ubiquitin ubiquitin JJ work_infoczas7nfbzac5mhjnxpkxle 405 27 C C NNP work_infoczas7nfbzac5mhjnxpkxle 405 28 promoter promoter NN work_infoczas7nfbzac5mhjnxpkxle 405 29 directs direct VBZ work_infoczas7nfbzac5mhjnxpkxle 405 30 high high JJ work_infoczas7nfbzac5mhjnxpkxle 405 31 ubiquitous ubiquitous JJ work_infoczas7nfbzac5mhjnxpkxle 405 32 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 405 33 of of IN work_infoczas7nfbzac5mhjnxpkxle 405 34 transgenes transgene NNS work_infoczas7nfbzac5mhjnxpkxle 405 35 in in IN work_infoczas7nfbzac5mhjnxpkxle 405 36 mice mouse NNS work_infoczas7nfbzac5mhjnxpkxle 405 37 . . . work_infoczas7nfbzac5mhjnxpkxle 406 1 Nucleic Nucleic NNP work_infoczas7nfbzac5mhjnxpkxle 406 2 Acids Acids NNPS work_infoczas7nfbzac5mhjnxpkxle 406 3 Res Res NNP work_infoczas7nfbzac5mhjnxpkxle 406 4 24:1787- 24:1787- NNP work_infoczas7nfbzac5mhjnxpkxle 406 5 1788 1788 CD work_infoczas7nfbzac5mhjnxpkxle 406 6 . . . work_infoczas7nfbzac5mhjnxpkxle 407 1 Schroder Schroder NNP work_infoczas7nfbzac5mhjnxpkxle 407 2 AR AR NNP work_infoczas7nfbzac5mhjnxpkxle 407 3 , , , work_infoczas7nfbzac5mhjnxpkxle 407 4 Shinn Shinn NNP work_infoczas7nfbzac5mhjnxpkxle 407 5 P P NNP work_infoczas7nfbzac5mhjnxpkxle 407 6 , , , work_infoczas7nfbzac5mhjnxpkxle 407 7 Chen Chen NNP work_infoczas7nfbzac5mhjnxpkxle 407 8 H H NNP work_infoczas7nfbzac5mhjnxpkxle 407 9 , , , work_infoczas7nfbzac5mhjnxpkxle 407 10 Berry Berry NNP work_infoczas7nfbzac5mhjnxpkxle 407 11 C C NNP work_infoczas7nfbzac5mhjnxpkxle 407 12 , , , work_infoczas7nfbzac5mhjnxpkxle 407 13 Ecker Ecker NNP work_infoczas7nfbzac5mhjnxpkxle 407 14 JR JR NNP work_infoczas7nfbzac5mhjnxpkxle 407 15 , , , work_infoczas7nfbzac5mhjnxpkxle 407 16 Bushman Bushman NNP work_infoczas7nfbzac5mhjnxpkxle 407 17 F F NNP work_infoczas7nfbzac5mhjnxpkxle 407 18 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 407 19 2002 2002 CD work_infoczas7nfbzac5mhjnxpkxle 407 20 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 407 21 HIV-1 HIV-1 NNP work_infoczas7nfbzac5mhjnxpkxle 407 22 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 407 23 in in IN work_infoczas7nfbzac5mhjnxpkxle 407 24 the the DT work_infoczas7nfbzac5mhjnxpkxle 407 25 human human JJ work_infoczas7nfbzac5mhjnxpkxle 407 26 genome genome NN work_infoczas7nfbzac5mhjnxpkxle 407 27 favors favor VBZ work_infoczas7nfbzac5mhjnxpkxle 407 28 active active JJ work_infoczas7nfbzac5mhjnxpkxle 407 29 genes gene NNS work_infoczas7nfbzac5mhjnxpkxle 407 30 and and CC work_infoczas7nfbzac5mhjnxpkxle 407 31 local local JJ work_infoczas7nfbzac5mhjnxpkxle 407 32 hotspots hotspot NNS work_infoczas7nfbzac5mhjnxpkxle 407 33 . . . work_infoczas7nfbzac5mhjnxpkxle 408 1 Cell Cell NNP work_infoczas7nfbzac5mhjnxpkxle 408 2 110:521 110:521 CD work_infoczas7nfbzac5mhjnxpkxle 408 3 - - SYM work_infoczas7nfbzac5mhjnxpkxle 408 4 529 529 CD work_infoczas7nfbzac5mhjnxpkxle 408 5 . . . work_infoczas7nfbzac5mhjnxpkxle 409 1 Singh Singh NNP work_infoczas7nfbzac5mhjnxpkxle 409 2 H H NNP work_infoczas7nfbzac5mhjnxpkxle 409 3 , , , work_infoczas7nfbzac5mhjnxpkxle 409 4 Manuri Manuri NNP work_infoczas7nfbzac5mhjnxpkxle 409 5 PR PR NNP work_infoczas7nfbzac5mhjnxpkxle 409 6 , , , work_infoczas7nfbzac5mhjnxpkxle 409 7 Olivares Olivares NNP work_infoczas7nfbzac5mhjnxpkxle 409 8 S S NNP work_infoczas7nfbzac5mhjnxpkxle 409 9 , , , work_infoczas7nfbzac5mhjnxpkxle 409 10 Dara Dara NNP work_infoczas7nfbzac5mhjnxpkxle 409 11 N N NNP work_infoczas7nfbzac5mhjnxpkxle 409 12 , , , work_infoczas7nfbzac5mhjnxpkxle 409 13 Dawson Dawson NNP work_infoczas7nfbzac5mhjnxpkxle 409 14 MJ MJ NNP work_infoczas7nfbzac5mhjnxpkxle 409 15 , , , work_infoczas7nfbzac5mhjnxpkxle 409 16 Huls Huls NNP work_infoczas7nfbzac5mhjnxpkxle 409 17 H H NNP work_infoczas7nfbzac5mhjnxpkxle 409 18 , , , work_infoczas7nfbzac5mhjnxpkxle 409 19 Hackett Hackett NNP work_infoczas7nfbzac5mhjnxpkxle 409 20 PB PB NNP work_infoczas7nfbzac5mhjnxpkxle 409 21 , , , work_infoczas7nfbzac5mhjnxpkxle 409 22 Kohn Kohn NNP work_infoczas7nfbzac5mhjnxpkxle 409 23 DB DB NNP work_infoczas7nfbzac5mhjnxpkxle 409 24 , , , work_infoczas7nfbzac5mhjnxpkxle 409 25 Shpall Shpall NNP work_infoczas7nfbzac5mhjnxpkxle 409 26 EJ EJ NNP work_infoczas7nfbzac5mhjnxpkxle 409 27 , , , work_infoczas7nfbzac5mhjnxpkxle 409 28 Champlin Champlin NNP work_infoczas7nfbzac5mhjnxpkxle 409 29 RE RE NNP work_infoczas7nfbzac5mhjnxpkxle 409 30 , , , work_infoczas7nfbzac5mhjnxpkxle 409 31 Cooper Cooper NNP work_infoczas7nfbzac5mhjnxpkxle 409 32 LJ LJ NNP work_infoczas7nfbzac5mhjnxpkxle 409 33 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 409 34 2008 2008 CD work_infoczas7nfbzac5mhjnxpkxle 409 35 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 409 36 Redirecting redirect VBG work_infoczas7nfbzac5mhjnxpkxle 409 37 specificity specificity NN work_infoczas7nfbzac5mhjnxpkxle 409 38 of of IN work_infoczas7nfbzac5mhjnxpkxle 409 39 T t NN work_infoczas7nfbzac5mhjnxpkxle 409 40 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 409 41 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 409 42 populations population NNS work_infoczas7nfbzac5mhjnxpkxle 409 43 for for IN work_infoczas7nfbzac5mhjnxpkxle 409 44 CD19 CD19 NNP work_infoczas7nfbzac5mhjnxpkxle 409 45 using use VBG work_infoczas7nfbzac5mhjnxpkxle 409 46 the the DT work_infoczas7nfbzac5mhjnxpkxle 409 47 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 409 48 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 409 49 system system NN work_infoczas7nfbzac5mhjnxpkxle 409 50 . . . work_infoczas7nfbzac5mhjnxpkxle 410 1 Cancer cancer NN work_infoczas7nfbzac5mhjnxpkxle 410 2 Res Res NNP work_infoczas7nfbzac5mhjnxpkxle 410 3 68:2961 68:2961 CD work_infoczas7nfbzac5mhjnxpkxle 410 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 410 5 2971 2971 CD work_infoczas7nfbzac5mhjnxpkxle 410 6 . . . work_infoczas7nfbzac5mhjnxpkxle 411 1 Sinzelle Sinzelle NNP work_infoczas7nfbzac5mhjnxpkxle 411 2 L L NNP work_infoczas7nfbzac5mhjnxpkxle 411 3 , , , work_infoczas7nfbzac5mhjnxpkxle 411 4 Vallin Vallin NNP work_infoczas7nfbzac5mhjnxpkxle 411 5 J J NNP work_infoczas7nfbzac5mhjnxpkxle 411 6 , , , work_infoczas7nfbzac5mhjnxpkxle 411 7 Coen Coen NNP work_infoczas7nfbzac5mhjnxpkxle 411 8 L L NNP work_infoczas7nfbzac5mhjnxpkxle 411 9 , , , work_infoczas7nfbzac5mhjnxpkxle 411 10 Chesneau Chesneau NNP work_infoczas7nfbzac5mhjnxpkxle 411 11 A A NNP work_infoczas7nfbzac5mhjnxpkxle 411 12 , , , work_infoczas7nfbzac5mhjnxpkxle 411 13 Du Du NNP work_infoczas7nfbzac5mhjnxpkxle 411 14 Pasquier Pasquier NNP work_infoczas7nfbzac5mhjnxpkxle 411 15 D D NNP work_infoczas7nfbzac5mhjnxpkxle 411 16 , , , work_infoczas7nfbzac5mhjnxpkxle 411 17 Pollet Pollet NNP work_infoczas7nfbzac5mhjnxpkxle 411 18 N N NNP work_infoczas7nfbzac5mhjnxpkxle 411 19 , , , work_infoczas7nfbzac5mhjnxpkxle 411 20 Demeneix Demeneix NNP work_infoczas7nfbzac5mhjnxpkxle 411 21 B B NNP work_infoczas7nfbzac5mhjnxpkxle 411 22 , , , work_infoczas7nfbzac5mhjnxpkxle 411 23 Mazabraud Mazabraud NNP work_infoczas7nfbzac5mhjnxpkxle 411 24 A A NNP work_infoczas7nfbzac5mhjnxpkxle 411 25 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 411 26 2006 2006 CD work_infoczas7nfbzac5mhjnxpkxle 411 27 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 411 28 Generation generation NN work_infoczas7nfbzac5mhjnxpkxle 411 29 of of IN work_infoczas7nfbzac5mhjnxpkxle 411 30 trangenic trangenic JJ work_infoczas7nfbzac5mhjnxpkxle 411 31 Xenopus Xenopus NNP work_infoczas7nfbzac5mhjnxpkxle 411 32 laevis laevi NNS work_infoczas7nfbzac5mhjnxpkxle 411 33 using use VBG work_infoczas7nfbzac5mhjnxpkxle 411 34 the the DT work_infoczas7nfbzac5mhjnxpkxle 411 35 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 411 36 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 411 37 transposon transposon NN work_infoczas7nfbzac5mhjnxpkxle 411 38 system system NN work_infoczas7nfbzac5mhjnxpkxle 411 39 . . . work_infoczas7nfbzac5mhjnxpkxle 412 1 Transgenic Transgenic NNP work_infoczas7nfbzac5mhjnxpkxle 412 2 Res Res NNP work_infoczas7nfbzac5mhjnxpkxle 412 3 15:751 15:751 CD work_infoczas7nfbzac5mhjnxpkxle 412 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 412 5 760 760 CD work_infoczas7nfbzac5mhjnxpkxle 412 6 . . . work_infoczas7nfbzac5mhjnxpkxle 413 1 27 27 CD work_infoczas7nfbzac5mhjnxpkxle 413 2 Staunstrup Staunstrup NNP work_infoczas7nfbzac5mhjnxpkxle 413 3 NH NH NNP work_infoczas7nfbzac5mhjnxpkxle 413 4 , , , work_infoczas7nfbzac5mhjnxpkxle 413 5 Moldt Moldt NNP work_infoczas7nfbzac5mhjnxpkxle 413 6 B B NNP work_infoczas7nfbzac5mhjnxpkxle 413 7 , , , work_infoczas7nfbzac5mhjnxpkxle 413 8 Mates Mates NNP work_infoczas7nfbzac5mhjnxpkxle 413 9 L L NNP work_infoczas7nfbzac5mhjnxpkxle 413 10 , , , work_infoczas7nfbzac5mhjnxpkxle 413 11 Villesen Villesen NNP work_infoczas7nfbzac5mhjnxpkxle 413 12 P P NNP work_infoczas7nfbzac5mhjnxpkxle 413 13 , , , work_infoczas7nfbzac5mhjnxpkxle 413 14 Jakobsen Jakobsen NNP work_infoczas7nfbzac5mhjnxpkxle 413 15 M M NNP work_infoczas7nfbzac5mhjnxpkxle 413 16 , , , work_infoczas7nfbzac5mhjnxpkxle 413 17 Ivics Ivics NNP work_infoczas7nfbzac5mhjnxpkxle 413 18 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 413 19 , , , work_infoczas7nfbzac5mhjnxpkxle 413 20 Izsvak Izsvak NNP work_infoczas7nfbzac5mhjnxpkxle 413 21 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 413 22 , , , work_infoczas7nfbzac5mhjnxpkxle 413 23 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 413 24 JG JG NNP work_infoczas7nfbzac5mhjnxpkxle 413 25 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 413 26 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 413 27 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 413 28 Hybrid hybrid JJ work_infoczas7nfbzac5mhjnxpkxle 413 29 lentivirus lentivirus NN work_infoczas7nfbzac5mhjnxpkxle 413 30 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 413 31 transposon transposon NN work_infoczas7nfbzac5mhjnxpkxle 413 32 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 413 33 with with IN work_infoczas7nfbzac5mhjnxpkxle 413 34 a a DT work_infoczas7nfbzac5mhjnxpkxle 413 35 random random JJ work_infoczas7nfbzac5mhjnxpkxle 413 36 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 413 37 profile profile NN work_infoczas7nfbzac5mhjnxpkxle 413 38 in in IN work_infoczas7nfbzac5mhjnxpkxle 413 39 human human JJ work_infoczas7nfbzac5mhjnxpkxle 413 40 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 413 41 . . . work_infoczas7nfbzac5mhjnxpkxle 414 1 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 414 2 Ther Ther NNP work_infoczas7nfbzac5mhjnxpkxle 414 3 17:1205 17:1205 CD work_infoczas7nfbzac5mhjnxpkxle 414 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 414 5 1214 1214 CD work_infoczas7nfbzac5mhjnxpkxle 414 6 . . . work_infoczas7nfbzac5mhjnxpkxle 415 1 Tolstrup Tolstrup NNP work_infoczas7nfbzac5mhjnxpkxle 415 2 AB AB NNP work_infoczas7nfbzac5mhjnxpkxle 415 3 , , , work_infoczas7nfbzac5mhjnxpkxle 415 4 Duch Duch NNP work_infoczas7nfbzac5mhjnxpkxle 415 5 M M NNP work_infoczas7nfbzac5mhjnxpkxle 415 6 , , , work_infoczas7nfbzac5mhjnxpkxle 415 7 Dalum Dalum NNP work_infoczas7nfbzac5mhjnxpkxle 415 8 I I NNP work_infoczas7nfbzac5mhjnxpkxle 415 9 , , , work_infoczas7nfbzac5mhjnxpkxle 415 10 Pedersen Pedersen NNP work_infoczas7nfbzac5mhjnxpkxle 415 11 FS FS NNP work_infoczas7nfbzac5mhjnxpkxle 415 12 , , , work_infoczas7nfbzac5mhjnxpkxle 415 13 Mouritsen Mouritsen NNP work_infoczas7nfbzac5mhjnxpkxle 415 14 S S NNP work_infoczas7nfbzac5mhjnxpkxle 415 15 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 415 16 2001 2001 CD work_infoczas7nfbzac5mhjnxpkxle 415 17 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 415 18 Functional functional JJ work_infoczas7nfbzac5mhjnxpkxle 415 19 screening screening NN work_infoczas7nfbzac5mhjnxpkxle 415 20 of of IN work_infoczas7nfbzac5mhjnxpkxle 415 21 a a DT work_infoczas7nfbzac5mhjnxpkxle 415 22 retroviral retroviral JJ work_infoczas7nfbzac5mhjnxpkxle 415 23 peptide peptide JJ work_infoczas7nfbzac5mhjnxpkxle 415 24 library library NN work_infoczas7nfbzac5mhjnxpkxle 415 25 for for IN work_infoczas7nfbzac5mhjnxpkxle 415 26 MHC MHC NNP work_infoczas7nfbzac5mhjnxpkxle 415 27 class class NN work_infoczas7nfbzac5mhjnxpkxle 415 28 I -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 415 29 presentation presentation NN work_infoczas7nfbzac5mhjnxpkxle 415 30 . . . work_infoczas7nfbzac5mhjnxpkxle 416 1 Gene Gene NNP work_infoczas7nfbzac5mhjnxpkxle 416 2 263:77 263:77 CD work_infoczas7nfbzac5mhjnxpkxle 416 3 - - SYM work_infoczas7nfbzac5mhjnxpkxle 416 4 84 84 CD work_infoczas7nfbzac5mhjnxpkxle 416 5 . . . work_infoczas7nfbzac5mhjnxpkxle 417 1 Vigdal Vigdal NNP work_infoczas7nfbzac5mhjnxpkxle 417 2 TJ TJ NNP work_infoczas7nfbzac5mhjnxpkxle 417 3 , , , work_infoczas7nfbzac5mhjnxpkxle 417 4 Kaufman Kaufman NNP work_infoczas7nfbzac5mhjnxpkxle 417 5 CD CD NNP work_infoczas7nfbzac5mhjnxpkxle 417 6 , , , work_infoczas7nfbzac5mhjnxpkxle 417 7 Izsvak Izsvak NNP work_infoczas7nfbzac5mhjnxpkxle 417 8 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 417 9 , , , work_infoczas7nfbzac5mhjnxpkxle 417 10 Voytas Voytas NNP work_infoczas7nfbzac5mhjnxpkxle 417 11 DF DF NNP work_infoczas7nfbzac5mhjnxpkxle 417 12 , , , work_infoczas7nfbzac5mhjnxpkxle 417 13 Ivics Ivics NNP work_infoczas7nfbzac5mhjnxpkxle 417 14 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 417 15 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 417 16 2002 2002 CD work_infoczas7nfbzac5mhjnxpkxle 417 17 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 417 18 Common common JJ work_infoczas7nfbzac5mhjnxpkxle 417 19 physical physical JJ work_infoczas7nfbzac5mhjnxpkxle 417 20 properties property NNS work_infoczas7nfbzac5mhjnxpkxle 417 21 of of IN work_infoczas7nfbzac5mhjnxpkxle 417 22 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 417 23 affecting affect VBG work_infoczas7nfbzac5mhjnxpkxle 417 24 target target NN work_infoczas7nfbzac5mhjnxpkxle 417 25 site site NN work_infoczas7nfbzac5mhjnxpkxle 417 26 selection selection NN work_infoczas7nfbzac5mhjnxpkxle 417 27 of of IN work_infoczas7nfbzac5mhjnxpkxle 417 28 sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 417 29 beauty beauty NN work_infoczas7nfbzac5mhjnxpkxle 417 30 and and CC work_infoczas7nfbzac5mhjnxpkxle 417 31 other other JJ work_infoczas7nfbzac5mhjnxpkxle 417 32 Tc1 Tc1 NNP work_infoczas7nfbzac5mhjnxpkxle 417 33 / / SYM work_infoczas7nfbzac5mhjnxpkxle 417 34 mariner mariner NN work_infoczas7nfbzac5mhjnxpkxle 417 35 transposable transposable JJ work_infoczas7nfbzac5mhjnxpkxle 417 36 elements element NNS work_infoczas7nfbzac5mhjnxpkxle 417 37 . . . work_infoczas7nfbzac5mhjnxpkxle 418 1 J J NNP work_infoczas7nfbzac5mhjnxpkxle 418 2 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 418 3 Biol Biol NNP work_infoczas7nfbzac5mhjnxpkxle 418 4 323:441 323:441 CD work_infoczas7nfbzac5mhjnxpkxle 418 5 - - SYM work_infoczas7nfbzac5mhjnxpkxle 418 6 452 452 CD work_infoczas7nfbzac5mhjnxpkxle 418 7 . . . work_infoczas7nfbzac5mhjnxpkxle 419 1 Wakayama Wakayama NNP work_infoczas7nfbzac5mhjnxpkxle 419 2 T T NNP work_infoczas7nfbzac5mhjnxpkxle 419 3 , , , work_infoczas7nfbzac5mhjnxpkxle 419 4 Shinkai Shinkai NNP work_infoczas7nfbzac5mhjnxpkxle 419 5 Y Y NNP work_infoczas7nfbzac5mhjnxpkxle 419 6 , , , work_infoczas7nfbzac5mhjnxpkxle 419 7 Tamashiro Tamashiro NNP work_infoczas7nfbzac5mhjnxpkxle 419 8 KL KL NNP work_infoczas7nfbzac5mhjnxpkxle 419 9 , , , work_infoczas7nfbzac5mhjnxpkxle 419 10 Niida Niida NNP work_infoczas7nfbzac5mhjnxpkxle 419 11 H H NNP work_infoczas7nfbzac5mhjnxpkxle 419 12 , , , work_infoczas7nfbzac5mhjnxpkxle 419 13 Blanchard Blanchard NNP work_infoczas7nfbzac5mhjnxpkxle 419 14 DC DC NNP work_infoczas7nfbzac5mhjnxpkxle 419 15 , , , work_infoczas7nfbzac5mhjnxpkxle 419 16 Blanchard Blanchard NNP work_infoczas7nfbzac5mhjnxpkxle 419 17 RJ RJ NNP work_infoczas7nfbzac5mhjnxpkxle 419 18 , , , work_infoczas7nfbzac5mhjnxpkxle 419 19 Ogura Ogura NNP work_infoczas7nfbzac5mhjnxpkxle 419 20 A A NNP work_infoczas7nfbzac5mhjnxpkxle 419 21 , , , work_infoczas7nfbzac5mhjnxpkxle 419 22 Tanemura Tanemura NNP work_infoczas7nfbzac5mhjnxpkxle 419 23 K K NNP work_infoczas7nfbzac5mhjnxpkxle 419 24 , , , work_infoczas7nfbzac5mhjnxpkxle 419 25 Tachibana Tachibana NNP work_infoczas7nfbzac5mhjnxpkxle 419 26 M M NNP work_infoczas7nfbzac5mhjnxpkxle 419 27 , , , work_infoczas7nfbzac5mhjnxpkxle 419 28 Perry Perry NNP work_infoczas7nfbzac5mhjnxpkxle 419 29 AC AC NNP work_infoczas7nfbzac5mhjnxpkxle 419 30 , , , work_infoczas7nfbzac5mhjnxpkxle 419 31 Colgan Colgan NNP work_infoczas7nfbzac5mhjnxpkxle 419 32 DF DF NNP work_infoczas7nfbzac5mhjnxpkxle 419 33 , , , work_infoczas7nfbzac5mhjnxpkxle 419 34 Mombaerts Mombaerts NNP work_infoczas7nfbzac5mhjnxpkxle 419 35 P P NNP work_infoczas7nfbzac5mhjnxpkxle 419 36 , , , work_infoczas7nfbzac5mhjnxpkxle 419 37 Yanagimachi Yanagimachi NNP work_infoczas7nfbzac5mhjnxpkxle 419 38 R R NNP work_infoczas7nfbzac5mhjnxpkxle 419 39 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 419 40 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 419 41 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 419 42 Cloning clone VBG work_infoczas7nfbzac5mhjnxpkxle 419 43 of of IN work_infoczas7nfbzac5mhjnxpkxle 419 44 mice mouse NNS work_infoczas7nfbzac5mhjnxpkxle 419 45 to to IN work_infoczas7nfbzac5mhjnxpkxle 419 46 six six CD work_infoczas7nfbzac5mhjnxpkxle 419 47 generations generation NNS work_infoczas7nfbzac5mhjnxpkxle 419 48 . . . work_infoczas7nfbzac5mhjnxpkxle 420 1 Nature nature NN work_infoczas7nfbzac5mhjnxpkxle 420 2 407:318 407:318 CD work_infoczas7nfbzac5mhjnxpkxle 420 3 - - SYM work_infoczas7nfbzac5mhjnxpkxle 420 4 319 319 CD work_infoczas7nfbzac5mhjnxpkxle 420 5 . . . work_infoczas7nfbzac5mhjnxpkxle 421 1 Wakayama Wakayama NNP work_infoczas7nfbzac5mhjnxpkxle 421 2 T T NNP work_infoczas7nfbzac5mhjnxpkxle 421 3 , , , work_infoczas7nfbzac5mhjnxpkxle 421 4 Yanagimachi Yanagimachi NNP work_infoczas7nfbzac5mhjnxpkxle 421 5 R R NNP work_infoczas7nfbzac5mhjnxpkxle 421 6 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 421 7 1999 1999 CD work_infoczas7nfbzac5mhjnxpkxle 421 8 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 421 9 Cloning clone VBG work_infoczas7nfbzac5mhjnxpkxle 421 10 of of IN work_infoczas7nfbzac5mhjnxpkxle 421 11 male male JJ work_infoczas7nfbzac5mhjnxpkxle 421 12 mice mouse NNS work_infoczas7nfbzac5mhjnxpkxle 421 13 from from IN work_infoczas7nfbzac5mhjnxpkxle 421 14 adult adult NN work_infoczas7nfbzac5mhjnxpkxle 421 15 tail tail NN work_infoczas7nfbzac5mhjnxpkxle 421 16 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 421 17 tip tip NN work_infoczas7nfbzac5mhjnxpkxle 421 18 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 421 19 . . . work_infoczas7nfbzac5mhjnxpkxle 422 1 Nat Nat NNP work_infoczas7nfbzac5mhjnxpkxle 422 2 Genet Genet NNP work_infoczas7nfbzac5mhjnxpkxle 422 3 22:127 22:127 CD work_infoczas7nfbzac5mhjnxpkxle 422 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 422 5 128 128 CD work_infoczas7nfbzac5mhjnxpkxle 422 6 . . . work_infoczas7nfbzac5mhjnxpkxle 423 1 Watanabe Watanabe NNP work_infoczas7nfbzac5mhjnxpkxle 423 2 S S NNP work_infoczas7nfbzac5mhjnxpkxle 423 3 , , , work_infoczas7nfbzac5mhjnxpkxle 423 4 Iwamoto Iwamoto NNP work_infoczas7nfbzac5mhjnxpkxle 423 5 M M NNP work_infoczas7nfbzac5mhjnxpkxle 423 6 , , , work_infoczas7nfbzac5mhjnxpkxle 423 7 Suzuki Suzuki NNP work_infoczas7nfbzac5mhjnxpkxle 423 8 S S NNP work_infoczas7nfbzac5mhjnxpkxle 423 9 , , , work_infoczas7nfbzac5mhjnxpkxle 423 10 Fuchimoto Fuchimoto NNP work_infoczas7nfbzac5mhjnxpkxle 423 11 D D NNP work_infoczas7nfbzac5mhjnxpkxle 423 12 , , , work_infoczas7nfbzac5mhjnxpkxle 423 13 Honma Honma NNP work_infoczas7nfbzac5mhjnxpkxle 423 14 D D NNP work_infoczas7nfbzac5mhjnxpkxle 423 15 , , , work_infoczas7nfbzac5mhjnxpkxle 423 16 Nagai Nagai NNP work_infoczas7nfbzac5mhjnxpkxle 423 17 T T NNP work_infoczas7nfbzac5mhjnxpkxle 423 18 , , , work_infoczas7nfbzac5mhjnxpkxle 423 19 Hashimoto Hashimoto NNP work_infoczas7nfbzac5mhjnxpkxle 423 20 M M NNP work_infoczas7nfbzac5mhjnxpkxle 423 21 , , , work_infoczas7nfbzac5mhjnxpkxle 423 22 Yazaki Yazaki NNP work_infoczas7nfbzac5mhjnxpkxle 423 23 S S NNP work_infoczas7nfbzac5mhjnxpkxle 423 24 , , , work_infoczas7nfbzac5mhjnxpkxle 423 25 Sato Sato NNP work_infoczas7nfbzac5mhjnxpkxle 423 26 M M NNP work_infoczas7nfbzac5mhjnxpkxle 423 27 , , , work_infoczas7nfbzac5mhjnxpkxle 423 28 Onishi Onishi NNP work_infoczas7nfbzac5mhjnxpkxle 423 29 A A NNP work_infoczas7nfbzac5mhjnxpkxle 423 30 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 423 31 2005 2005 CD work_infoczas7nfbzac5mhjnxpkxle 423 32 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 423 33 A a DT work_infoczas7nfbzac5mhjnxpkxle 423 34 novel novel JJ work_infoczas7nfbzac5mhjnxpkxle 423 35 method method NN work_infoczas7nfbzac5mhjnxpkxle 423 36 for for IN work_infoczas7nfbzac5mhjnxpkxle 423 37 the the DT work_infoczas7nfbzac5mhjnxpkxle 423 38 production production NN work_infoczas7nfbzac5mhjnxpkxle 423 39 of of IN work_infoczas7nfbzac5mhjnxpkxle 423 40 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 423 41 cloned clone VBN work_infoczas7nfbzac5mhjnxpkxle 423 42 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 423 43 : : : work_infoczas7nfbzac5mhjnxpkxle 423 44 electroporation- electroporation- NNP work_infoczas7nfbzac5mhjnxpkxle 423 45 mediated mediate VBD work_infoczas7nfbzac5mhjnxpkxle 423 46 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 423 47 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 423 48 to to IN work_infoczas7nfbzac5mhjnxpkxle 423 49 non non JJ work_infoczas7nfbzac5mhjnxpkxle 423 50 - - JJ work_infoczas7nfbzac5mhjnxpkxle 423 51 cultured cultured JJ work_infoczas7nfbzac5mhjnxpkxle 423 52 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 423 53 and and CC work_infoczas7nfbzac5mhjnxpkxle 423 54 subsequent subsequent JJ work_infoczas7nfbzac5mhjnxpkxle 423 55 selection selection NN work_infoczas7nfbzac5mhjnxpkxle 423 56 with with IN work_infoczas7nfbzac5mhjnxpkxle 423 57 puromycin puromycin NNS work_infoczas7nfbzac5mhjnxpkxle 423 58 . . . work_infoczas7nfbzac5mhjnxpkxle 424 1 Biol Biol NNP work_infoczas7nfbzac5mhjnxpkxle 424 2 Reprod Reprod NNP work_infoczas7nfbzac5mhjnxpkxle 424 3 72:309 72:309 CD work_infoczas7nfbzac5mhjnxpkxle 424 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 424 5 315 315 CD work_infoczas7nfbzac5mhjnxpkxle 424 6 . . . work_infoczas7nfbzac5mhjnxpkxle 425 1 Webster Webster NNP work_infoczas7nfbzac5mhjnxpkxle 425 2 NL NL NNP work_infoczas7nfbzac5mhjnxpkxle 425 3 , , , work_infoczas7nfbzac5mhjnxpkxle 425 4 Forni Forni NNP work_infoczas7nfbzac5mhjnxpkxle 425 5 M M NNP work_infoczas7nfbzac5mhjnxpkxle 425 6 , , , work_infoczas7nfbzac5mhjnxpkxle 425 7 Bacci Bacci NNP work_infoczas7nfbzac5mhjnxpkxle 425 8 ML ML NNP work_infoczas7nfbzac5mhjnxpkxle 425 9 , , , work_infoczas7nfbzac5mhjnxpkxle 425 10 Giovannoni Giovannoni NNP work_infoczas7nfbzac5mhjnxpkxle 425 11 R R NNP work_infoczas7nfbzac5mhjnxpkxle 425 12 , , , work_infoczas7nfbzac5mhjnxpkxle 425 13 Razzini Razzini NNP work_infoczas7nfbzac5mhjnxpkxle 425 14 R R NNP work_infoczas7nfbzac5mhjnxpkxle 425 15 , , , work_infoczas7nfbzac5mhjnxpkxle 425 16 Fantinati Fantinati NNP work_infoczas7nfbzac5mhjnxpkxle 425 17 P P NNP work_infoczas7nfbzac5mhjnxpkxle 425 18 , , , work_infoczas7nfbzac5mhjnxpkxle 425 19 Zannoni Zannoni NNP work_infoczas7nfbzac5mhjnxpkxle 425 20 A A NNP work_infoczas7nfbzac5mhjnxpkxle 425 21 , , , work_infoczas7nfbzac5mhjnxpkxle 425 22 Fusetti Fusetti NNP work_infoczas7nfbzac5mhjnxpkxle 425 23 L L NNP work_infoczas7nfbzac5mhjnxpkxle 425 24 , , , work_infoczas7nfbzac5mhjnxpkxle 425 25 Dalpra Dalpra NNP work_infoczas7nfbzac5mhjnxpkxle 425 26 L L NNP work_infoczas7nfbzac5mhjnxpkxle 425 27 , , , work_infoczas7nfbzac5mhjnxpkxle 425 28 Bianco Bianco NNP work_infoczas7nfbzac5mhjnxpkxle 425 29 MR MR NNP work_infoczas7nfbzac5mhjnxpkxle 425 30 , , , work_infoczas7nfbzac5mhjnxpkxle 425 31 Papa Papa NNP work_infoczas7nfbzac5mhjnxpkxle 425 32 M M NNP work_infoczas7nfbzac5mhjnxpkxle 425 33 , , , work_infoczas7nfbzac5mhjnxpkxle 425 34 Seren Seren NNP work_infoczas7nfbzac5mhjnxpkxle 425 35 E E NNP work_infoczas7nfbzac5mhjnxpkxle 425 36 , , , work_infoczas7nfbzac5mhjnxpkxle 425 37 Sandrin Sandrin NNP work_infoczas7nfbzac5mhjnxpkxle 425 38 MS MS NNP work_infoczas7nfbzac5mhjnxpkxle 425 39 , , , work_infoczas7nfbzac5mhjnxpkxle 425 40 Mc Mc NNP work_infoczas7nfbzac5mhjnxpkxle 425 41 Kenzie Kenzie NNP work_infoczas7nfbzac5mhjnxpkxle 425 42 IF IF NNP work_infoczas7nfbzac5mhjnxpkxle 425 43 , , , work_infoczas7nfbzac5mhjnxpkxle 425 44 Lavitrano Lavitrano NNP work_infoczas7nfbzac5mhjnxpkxle 425 45 M M NNP work_infoczas7nfbzac5mhjnxpkxle 425 46 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 425 47 2005 2005 CD work_infoczas7nfbzac5mhjnxpkxle 425 48 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 425 49 Multi multi JJ work_infoczas7nfbzac5mhjnxpkxle 425 50 - - JJ work_infoczas7nfbzac5mhjnxpkxle 425 51 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 425 52 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 425 53 expressing express VBG work_infoczas7nfbzac5mhjnxpkxle 425 54 three three CD work_infoczas7nfbzac5mhjnxpkxle 425 55 fluorescent fluorescent NN work_infoczas7nfbzac5mhjnxpkxle 425 56 proteins protein NNS work_infoczas7nfbzac5mhjnxpkxle 425 57 produced produce VBN work_infoczas7nfbzac5mhjnxpkxle 425 58 with with IN work_infoczas7nfbzac5mhjnxpkxle 425 59 high high JJ work_infoczas7nfbzac5mhjnxpkxle 425 60 efficiency efficiency NN work_infoczas7nfbzac5mhjnxpkxle 425 61 by by IN work_infoczas7nfbzac5mhjnxpkxle 425 62 sperm sperm JJ work_infoczas7nfbzac5mhjnxpkxle 425 63 mediated mediate VBN work_infoczas7nfbzac5mhjnxpkxle 425 64 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 425 65 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 425 66 . . . work_infoczas7nfbzac5mhjnxpkxle 426 1 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 426 2 Reprod Reprod NNP work_infoczas7nfbzac5mhjnxpkxle 426 3 Dev Dev NNP work_infoczas7nfbzac5mhjnxpkxle 426 4 72:68 72:68 CD work_infoczas7nfbzac5mhjnxpkxle 426 5 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 426 6 76 76 CD work_infoczas7nfbzac5mhjnxpkxle 426 7 . . . work_infoczas7nfbzac5mhjnxpkxle 427 1 Whitelaw Whitelaw NNP work_infoczas7nfbzac5mhjnxpkxle 427 2 CB CB NNP work_infoczas7nfbzac5mhjnxpkxle 427 3 , , , work_infoczas7nfbzac5mhjnxpkxle 427 4 Radcliffe Radcliffe NNP work_infoczas7nfbzac5mhjnxpkxle 427 5 PA PA NNP work_infoczas7nfbzac5mhjnxpkxle 427 6 , , , work_infoczas7nfbzac5mhjnxpkxle 427 7 Ritchie Ritchie NNP work_infoczas7nfbzac5mhjnxpkxle 427 8 WA WA NNP work_infoczas7nfbzac5mhjnxpkxle 427 9 , , , work_infoczas7nfbzac5mhjnxpkxle 427 10 Carlisle Carlisle NNP work_infoczas7nfbzac5mhjnxpkxle 427 11 A A NNP work_infoczas7nfbzac5mhjnxpkxle 427 12 , , , work_infoczas7nfbzac5mhjnxpkxle 427 13 Ellard Ellard NNP work_infoczas7nfbzac5mhjnxpkxle 427 14 FM FM NNP work_infoczas7nfbzac5mhjnxpkxle 427 15 , , , work_infoczas7nfbzac5mhjnxpkxle 427 16 Pena Pena NNP work_infoczas7nfbzac5mhjnxpkxle 427 17 RN RN NNP work_infoczas7nfbzac5mhjnxpkxle 427 18 , , , work_infoczas7nfbzac5mhjnxpkxle 427 19 Rowe Rowe NNP work_infoczas7nfbzac5mhjnxpkxle 427 20 J J NNP work_infoczas7nfbzac5mhjnxpkxle 427 21 , , , work_infoczas7nfbzac5mhjnxpkxle 427 22 Clark Clark NNP work_infoczas7nfbzac5mhjnxpkxle 427 23 AJ AJ NNP work_infoczas7nfbzac5mhjnxpkxle 427 24 , , , work_infoczas7nfbzac5mhjnxpkxle 427 25 King King NNP work_infoczas7nfbzac5mhjnxpkxle 427 26 TJ TJ NNP work_infoczas7nfbzac5mhjnxpkxle 427 27 , , , work_infoczas7nfbzac5mhjnxpkxle 427 28 Mitrophanous Mitrophanous NNP work_infoczas7nfbzac5mhjnxpkxle 427 29 KA KA NNP work_infoczas7nfbzac5mhjnxpkxle 427 30 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 427 31 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 427 32 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 427 33 Efficient efficient JJ work_infoczas7nfbzac5mhjnxpkxle 427 34 generation generation NN work_infoczas7nfbzac5mhjnxpkxle 427 35 of of IN work_infoczas7nfbzac5mhjnxpkxle 427 36 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 427 37 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 427 38 using use VBG work_infoczas7nfbzac5mhjnxpkxle 427 39 equine equine NN work_infoczas7nfbzac5mhjnxpkxle 427 40 infectious infectious JJ work_infoczas7nfbzac5mhjnxpkxle 427 41 anaemia anaemia NN work_infoczas7nfbzac5mhjnxpkxle 427 42 virus virus NN work_infoczas7nfbzac5mhjnxpkxle 427 43 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 427 44 EIAV EIAV NNP work_infoczas7nfbzac5mhjnxpkxle 427 45 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 427 46 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 427 47 vector vector NN work_infoczas7nfbzac5mhjnxpkxle 427 48 . . . work_infoczas7nfbzac5mhjnxpkxle 428 1 FEBS FEBS NNP work_infoczas7nfbzac5mhjnxpkxle 428 2 Lett Lett NNP work_infoczas7nfbzac5mhjnxpkxle 428 3 571:233 571:233 CD work_infoczas7nfbzac5mhjnxpkxle 428 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 428 5 236 236 CD work_infoczas7nfbzac5mhjnxpkxle 428 6 . . . work_infoczas7nfbzac5mhjnxpkxle 429 1 28 28 CD work_infoczas7nfbzac5mhjnxpkxle 429 2 Wilber Wilber NNP work_infoczas7nfbzac5mhjnxpkxle 429 3 A A NNP work_infoczas7nfbzac5mhjnxpkxle 429 4 , , , work_infoczas7nfbzac5mhjnxpkxle 429 5 Frandsen Frandsen NNP work_infoczas7nfbzac5mhjnxpkxle 429 6 JL JL NNP work_infoczas7nfbzac5mhjnxpkxle 429 7 , , , work_infoczas7nfbzac5mhjnxpkxle 429 8 Geurts Geurts NNP work_infoczas7nfbzac5mhjnxpkxle 429 9 JL JL NNP work_infoczas7nfbzac5mhjnxpkxle 429 10 , , , work_infoczas7nfbzac5mhjnxpkxle 429 11 Largaespada Largaespada NNP work_infoczas7nfbzac5mhjnxpkxle 429 12 DA DA NNP work_infoczas7nfbzac5mhjnxpkxle 429 13 , , , work_infoczas7nfbzac5mhjnxpkxle 429 14 Hackett Hackett NNP work_infoczas7nfbzac5mhjnxpkxle 429 15 PB PB NNP work_infoczas7nfbzac5mhjnxpkxle 429 16 , , , work_infoczas7nfbzac5mhjnxpkxle 429 17 McIvor McIvor NNP work_infoczas7nfbzac5mhjnxpkxle 429 18 RS RS NNP work_infoczas7nfbzac5mhjnxpkxle 429 19 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 429 20 2006 2006 CD work_infoczas7nfbzac5mhjnxpkxle 429 21 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 429 22 RNA RNA NNP work_infoczas7nfbzac5mhjnxpkxle 429 23 as as IN work_infoczas7nfbzac5mhjnxpkxle 429 24 a a DT work_infoczas7nfbzac5mhjnxpkxle 429 25 source source NN work_infoczas7nfbzac5mhjnxpkxle 429 26 of of IN work_infoczas7nfbzac5mhjnxpkxle 429 27 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 429 28 for for IN work_infoczas7nfbzac5mhjnxpkxle 429 29 sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 429 30 beauty beauty NN work_infoczas7nfbzac5mhjnxpkxle 429 31 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 429 32 mediated mediate VBN work_infoczas7nfbzac5mhjnxpkxle 429 33 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 429 34 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 429 35 and and CC work_infoczas7nfbzac5mhjnxpkxle 429 36 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 429 37 in in IN work_infoczas7nfbzac5mhjnxpkxle 429 38 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 429 39 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 429 40 and and CC work_infoczas7nfbzac5mhjnxpkxle 429 41 tissues tissue NNS work_infoczas7nfbzac5mhjnxpkxle 429 42 . . . work_infoczas7nfbzac5mhjnxpkxle 430 1 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 430 2 Ther Ther NNP work_infoczas7nfbzac5mhjnxpkxle 430 3 13:625 13:625 CD work_infoczas7nfbzac5mhjnxpkxle 430 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 430 5 630 630 CD work_infoczas7nfbzac5mhjnxpkxle 430 6 . . . work_infoczas7nfbzac5mhjnxpkxle 431 1 Wilmut Wilmut NNP work_infoczas7nfbzac5mhjnxpkxle 431 2 I I NNP work_infoczas7nfbzac5mhjnxpkxle 431 3 , , , work_infoczas7nfbzac5mhjnxpkxle 431 4 Schnieke Schnieke NNP work_infoczas7nfbzac5mhjnxpkxle 431 5 AE AE NNP work_infoczas7nfbzac5mhjnxpkxle 431 6 , , , work_infoczas7nfbzac5mhjnxpkxle 431 7 McWhir McWhir NNP work_infoczas7nfbzac5mhjnxpkxle 431 8 J J NNP work_infoczas7nfbzac5mhjnxpkxle 431 9 , , , work_infoczas7nfbzac5mhjnxpkxle 431 10 Kind Kind NNP work_infoczas7nfbzac5mhjnxpkxle 431 11 AJ AJ NNP work_infoczas7nfbzac5mhjnxpkxle 431 12 , , , work_infoczas7nfbzac5mhjnxpkxle 431 13 Campbell Campbell NNP work_infoczas7nfbzac5mhjnxpkxle 431 14 KH KH NNP work_infoczas7nfbzac5mhjnxpkxle 431 15 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 431 16 1997 1997 CD work_infoczas7nfbzac5mhjnxpkxle 431 17 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 431 18 Viable viable JJ work_infoczas7nfbzac5mhjnxpkxle 431 19 offspring offspring NN work_infoczas7nfbzac5mhjnxpkxle 431 20 derived derive VBN work_infoczas7nfbzac5mhjnxpkxle 431 21 from from IN work_infoczas7nfbzac5mhjnxpkxle 431 22 fetal fetal JJ work_infoczas7nfbzac5mhjnxpkxle 431 23 and and CC work_infoczas7nfbzac5mhjnxpkxle 431 24 adult adult NN work_infoczas7nfbzac5mhjnxpkxle 431 25 mammalian mammalian JJ work_infoczas7nfbzac5mhjnxpkxle 431 26 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 431 27 . . . work_infoczas7nfbzac5mhjnxpkxle 432 1 Nature Nature NNP work_infoczas7nfbzac5mhjnxpkxle 432 2 385:810 385:810 CD work_infoczas7nfbzac5mhjnxpkxle 432 3 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 432 4 813 813 CD work_infoczas7nfbzac5mhjnxpkxle 432 5 . . . work_infoczas7nfbzac5mhjnxpkxle 433 1 Yant yant JJ work_infoczas7nfbzac5mhjnxpkxle 433 2 SR SR NNP work_infoczas7nfbzac5mhjnxpkxle 433 3 , , , work_infoczas7nfbzac5mhjnxpkxle 433 4 Meuse Meuse NNP work_infoczas7nfbzac5mhjnxpkxle 433 5 L L NNP work_infoczas7nfbzac5mhjnxpkxle 433 6 , , , work_infoczas7nfbzac5mhjnxpkxle 433 7 Chiu Chiu NNP work_infoczas7nfbzac5mhjnxpkxle 433 8 W W NNP work_infoczas7nfbzac5mhjnxpkxle 433 9 , , , work_infoczas7nfbzac5mhjnxpkxle 433 10 Ivics Ivics NNP work_infoczas7nfbzac5mhjnxpkxle 433 11 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 433 12 , , , work_infoczas7nfbzac5mhjnxpkxle 433 13 Izsvak Izsvak NNP work_infoczas7nfbzac5mhjnxpkxle 433 14 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 433 15 , , , work_infoczas7nfbzac5mhjnxpkxle 433 16 Kay Kay NNP work_infoczas7nfbzac5mhjnxpkxle 433 17 MA MA NNP work_infoczas7nfbzac5mhjnxpkxle 433 18 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 433 19 2000 2000 CD work_infoczas7nfbzac5mhjnxpkxle 433 20 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 433 21 Somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 433 22 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 433 23 and and CC work_infoczas7nfbzac5mhjnxpkxle 433 24 long long JJ work_infoczas7nfbzac5mhjnxpkxle 433 25 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 433 26 term term NN work_infoczas7nfbzac5mhjnxpkxle 433 27 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 433 28 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 433 29 in in IN work_infoczas7nfbzac5mhjnxpkxle 433 30 normal normal JJ work_infoczas7nfbzac5mhjnxpkxle 433 31 and and CC work_infoczas7nfbzac5mhjnxpkxle 433 32 haemophilic haemophilic JJ work_infoczas7nfbzac5mhjnxpkxle 433 33 mice mouse NNS work_infoczas7nfbzac5mhjnxpkxle 433 34 using use VBG work_infoczas7nfbzac5mhjnxpkxle 433 35 a a DT work_infoczas7nfbzac5mhjnxpkxle 433 36 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 433 37 transposon transposon NN work_infoczas7nfbzac5mhjnxpkxle 433 38 system system NN work_infoczas7nfbzac5mhjnxpkxle 433 39 . . . work_infoczas7nfbzac5mhjnxpkxle 434 1 Nat Nat NNP work_infoczas7nfbzac5mhjnxpkxle 434 2 Genet Genet NNP work_infoczas7nfbzac5mhjnxpkxle 434 3 25:35 25:35 CD work_infoczas7nfbzac5mhjnxpkxle 434 4 - - SYM work_infoczas7nfbzac5mhjnxpkxle 434 5 41 41 CD work_infoczas7nfbzac5mhjnxpkxle 434 6 . . . work_infoczas7nfbzac5mhjnxpkxle 435 1 Yant yant JJ work_infoczas7nfbzac5mhjnxpkxle 435 2 SR SR NNP work_infoczas7nfbzac5mhjnxpkxle 435 3 , , , work_infoczas7nfbzac5mhjnxpkxle 435 4 Park Park NNP work_infoczas7nfbzac5mhjnxpkxle 435 5 J J NNP work_infoczas7nfbzac5mhjnxpkxle 435 6 , , , work_infoczas7nfbzac5mhjnxpkxle 435 7 Huang Huang NNP work_infoczas7nfbzac5mhjnxpkxle 435 8 Y Y NNP work_infoczas7nfbzac5mhjnxpkxle 435 9 , , , work_infoczas7nfbzac5mhjnxpkxle 435 10 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 435 11 JG JG NNP work_infoczas7nfbzac5mhjnxpkxle 435 12 , , , work_infoczas7nfbzac5mhjnxpkxle 435 13 Kay Kay NNP work_infoczas7nfbzac5mhjnxpkxle 435 14 MA MA NNP work_infoczas7nfbzac5mhjnxpkxle 435 15 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 435 16 2004 2004 CD work_infoczas7nfbzac5mhjnxpkxle 435 17 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 435 18 Mutational mutational JJ work_infoczas7nfbzac5mhjnxpkxle 435 19 analysis analysis NN work_infoczas7nfbzac5mhjnxpkxle 435 20 of of IN work_infoczas7nfbzac5mhjnxpkxle 435 21 the the DT work_infoczas7nfbzac5mhjnxpkxle 435 22 N N NNP work_infoczas7nfbzac5mhjnxpkxle 435 23 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 435 24 terminal terminal NN work_infoczas7nfbzac5mhjnxpkxle 435 25 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 435 26 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 435 27 binding bind VBG work_infoczas7nfbzac5mhjnxpkxle 435 28 domain domain NN work_infoczas7nfbzac5mhjnxpkxle 435 29 of of IN work_infoczas7nfbzac5mhjnxpkxle 435 30 sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 435 31 beauty beauty NN work_infoczas7nfbzac5mhjnxpkxle 435 32 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 435 33 : : : work_infoczas7nfbzac5mhjnxpkxle 435 34 critical critical JJ work_infoczas7nfbzac5mhjnxpkxle 435 35 residues residue NNS work_infoczas7nfbzac5mhjnxpkxle 435 36 for for IN work_infoczas7nfbzac5mhjnxpkxle 435 37 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 435 38 binding bind VBG work_infoczas7nfbzac5mhjnxpkxle 435 39 and and CC work_infoczas7nfbzac5mhjnxpkxle 435 40 hyperactivity hyperactivity NN work_infoczas7nfbzac5mhjnxpkxle 435 41 in in IN work_infoczas7nfbzac5mhjnxpkxle 435 42 mammalian mammalian JJ work_infoczas7nfbzac5mhjnxpkxle 435 43 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 435 44 . . . work_infoczas7nfbzac5mhjnxpkxle 436 1 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 436 2 Cell Cell NNP work_infoczas7nfbzac5mhjnxpkxle 436 3 Biol Biol NNP work_infoczas7nfbzac5mhjnxpkxle 436 4 24:9239 24:9239 CD work_infoczas7nfbzac5mhjnxpkxle 436 5 - - SYM work_infoczas7nfbzac5mhjnxpkxle 436 6 9247 9247 CD work_infoczas7nfbzac5mhjnxpkxle 436 7 . . . work_infoczas7nfbzac5mhjnxpkxle 437 1 Yant yant JJ work_infoczas7nfbzac5mhjnxpkxle 437 2 SR SR NNP work_infoczas7nfbzac5mhjnxpkxle 437 3 , , , work_infoczas7nfbzac5mhjnxpkxle 437 4 Wu Wu NNP work_infoczas7nfbzac5mhjnxpkxle 437 5 X X NNP work_infoczas7nfbzac5mhjnxpkxle 437 6 , , , work_infoczas7nfbzac5mhjnxpkxle 437 7 Huang Huang NNP work_infoczas7nfbzac5mhjnxpkxle 437 8 Y Y NNP work_infoczas7nfbzac5mhjnxpkxle 437 9 , , , work_infoczas7nfbzac5mhjnxpkxle 437 10 Garrison Garrison NNP work_infoczas7nfbzac5mhjnxpkxle 437 11 B B NNP work_infoczas7nfbzac5mhjnxpkxle 437 12 , , , work_infoczas7nfbzac5mhjnxpkxle 437 13 Burgess Burgess NNP work_infoczas7nfbzac5mhjnxpkxle 437 14 SM SM NNP work_infoczas7nfbzac5mhjnxpkxle 437 15 , , , work_infoczas7nfbzac5mhjnxpkxle 437 16 Kay Kay NNP work_infoczas7nfbzac5mhjnxpkxle 437 17 MA MA NNP work_infoczas7nfbzac5mhjnxpkxle 437 18 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 437 19 2005 2005 CD work_infoczas7nfbzac5mhjnxpkxle 437 20 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 437 21 High high JJ work_infoczas7nfbzac5mhjnxpkxle 437 22 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 437 23 resolution resolution NN work_infoczas7nfbzac5mhjnxpkxle 437 24 genome genome NN work_infoczas7nfbzac5mhjnxpkxle 437 25 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 437 26 wide wide JJ work_infoczas7nfbzac5mhjnxpkxle 437 27 mapping mapping NN work_infoczas7nfbzac5mhjnxpkxle 437 28 of of IN work_infoczas7nfbzac5mhjnxpkxle 437 29 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 437 30 integration integration NN work_infoczas7nfbzac5mhjnxpkxle 437 31 in in IN work_infoczas7nfbzac5mhjnxpkxle 437 32 mammals mammal NNS work_infoczas7nfbzac5mhjnxpkxle 437 33 . . . work_infoczas7nfbzac5mhjnxpkxle 438 1 Mol Mol NNP work_infoczas7nfbzac5mhjnxpkxle 438 2 Cell Cell NNP work_infoczas7nfbzac5mhjnxpkxle 438 3 Biol Biol NNP work_infoczas7nfbzac5mhjnxpkxle 438 4 25:2085 25:2085 CD work_infoczas7nfbzac5mhjnxpkxle 438 5 - - SYM work_infoczas7nfbzac5mhjnxpkxle 438 6 2094 2094 CD work_infoczas7nfbzac5mhjnxpkxle 438 7 . . . work_infoczas7nfbzac5mhjnxpkxle 439 1 Yergeau Yergeau NNP work_infoczas7nfbzac5mhjnxpkxle 439 2 DA DA NNP work_infoczas7nfbzac5mhjnxpkxle 439 3 , , , work_infoczas7nfbzac5mhjnxpkxle 439 4 Johnson Johnson NNP work_infoczas7nfbzac5mhjnxpkxle 439 5 Hamlet Hamlet NNP work_infoczas7nfbzac5mhjnxpkxle 439 6 MR MR NNP work_infoczas7nfbzac5mhjnxpkxle 439 7 , , , work_infoczas7nfbzac5mhjnxpkxle 439 8 Kuliyev Kuliyev NNP work_infoczas7nfbzac5mhjnxpkxle 439 9 E E NNP work_infoczas7nfbzac5mhjnxpkxle 439 10 , , , work_infoczas7nfbzac5mhjnxpkxle 439 11 Zhu Zhu NNP work_infoczas7nfbzac5mhjnxpkxle 439 12 H H NNP work_infoczas7nfbzac5mhjnxpkxle 439 13 , , , work_infoczas7nfbzac5mhjnxpkxle 439 14 Doherty Doherty NNP work_infoczas7nfbzac5mhjnxpkxle 439 15 JR JR NNP work_infoczas7nfbzac5mhjnxpkxle 439 16 , , , work_infoczas7nfbzac5mhjnxpkxle 439 17 Archer Archer NNP work_infoczas7nfbzac5mhjnxpkxle 439 18 TD TD NNP work_infoczas7nfbzac5mhjnxpkxle 439 19 , , , work_infoczas7nfbzac5mhjnxpkxle 439 20 Subhawong Subhawong NNP work_infoczas7nfbzac5mhjnxpkxle 439 21 AP AP NNP work_infoczas7nfbzac5mhjnxpkxle 439 22 , , , work_infoczas7nfbzac5mhjnxpkxle 439 23 Valentine Valentine NNP work_infoczas7nfbzac5mhjnxpkxle 439 24 MB MB NNP work_infoczas7nfbzac5mhjnxpkxle 439 25 , , , work_infoczas7nfbzac5mhjnxpkxle 439 26 Kelley Kelley NNP work_infoczas7nfbzac5mhjnxpkxle 439 27 CM CM NNP work_infoczas7nfbzac5mhjnxpkxle 439 28 , , , work_infoczas7nfbzac5mhjnxpkxle 439 29 Mead Mead NNP work_infoczas7nfbzac5mhjnxpkxle 439 30 PE PE NNP work_infoczas7nfbzac5mhjnxpkxle 439 31 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 439 32 2009 2009 CD work_infoczas7nfbzac5mhjnxpkxle 439 33 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 439 34 Transgenesis Transgenesis NNP work_infoczas7nfbzac5mhjnxpkxle 439 35 in in IN work_infoczas7nfbzac5mhjnxpkxle 439 36 Xenopus Xenopus NNP work_infoczas7nfbzac5mhjnxpkxle 439 37 using use VBG work_infoczas7nfbzac5mhjnxpkxle 439 38 the the DT work_infoczas7nfbzac5mhjnxpkxle 439 39 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 439 40 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 439 41 transposon transposon NN work_infoczas7nfbzac5mhjnxpkxle 439 42 system system NN work_infoczas7nfbzac5mhjnxpkxle 439 43 . . . work_infoczas7nfbzac5mhjnxpkxle 440 1 Dev Dev NNP work_infoczas7nfbzac5mhjnxpkxle 440 2 Dyn Dyn NNP work_infoczas7nfbzac5mhjnxpkxle 440 3 238:1727 238:1727 NNP work_infoczas7nfbzac5mhjnxpkxle 440 4 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 440 5 1743 1743 CD work_infoczas7nfbzac5mhjnxpkxle 440 6 . . . work_infoczas7nfbzac5mhjnxpkxle 441 1 Zayed Zayed NNP work_infoczas7nfbzac5mhjnxpkxle 441 2 H H NNP work_infoczas7nfbzac5mhjnxpkxle 441 3 , , , work_infoczas7nfbzac5mhjnxpkxle 441 4 Izsvak Izsvak NNP work_infoczas7nfbzac5mhjnxpkxle 441 5 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 441 6 , , , work_infoczas7nfbzac5mhjnxpkxle 441 7 Khare Khare NNP work_infoczas7nfbzac5mhjnxpkxle 441 8 D D NNP work_infoczas7nfbzac5mhjnxpkxle 441 9 , , , work_infoczas7nfbzac5mhjnxpkxle 441 10 Heinemann Heinemann NNP work_infoczas7nfbzac5mhjnxpkxle 441 11 U U NNP work_infoczas7nfbzac5mhjnxpkxle 441 12 , , , work_infoczas7nfbzac5mhjnxpkxle 441 13 Ivics Ivics NNP work_infoczas7nfbzac5mhjnxpkxle 441 14 Z Z NNP work_infoczas7nfbzac5mhjnxpkxle 441 15 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 441 16 2003 2003 CD work_infoczas7nfbzac5mhjnxpkxle 441 17 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 441 18 The the DT work_infoczas7nfbzac5mhjnxpkxle 441 19 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 441 20 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 441 21 bending bend VBG work_infoczas7nfbzac5mhjnxpkxle 441 22 protein protein NN work_infoczas7nfbzac5mhjnxpkxle 441 23 HMGB1 HMGB1 NNP work_infoczas7nfbzac5mhjnxpkxle 441 24 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 441 25 a a DT work_infoczas7nfbzac5mhjnxpkxle 441 26 cellular cellular JJ work_infoczas7nfbzac5mhjnxpkxle 441 27 cofactor cofactor NN work_infoczas7nfbzac5mhjnxpkxle 441 28 of of IN work_infoczas7nfbzac5mhjnxpkxle 441 29 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 441 30 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 441 31 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 441 32 . . . work_infoczas7nfbzac5mhjnxpkxle 442 1 Nucleic Nucleic NNP work_infoczas7nfbzac5mhjnxpkxle 442 2 Acids Acids NNPS work_infoczas7nfbzac5mhjnxpkxle 442 3 Res Res NNP work_infoczas7nfbzac5mhjnxpkxle 442 4 31:2313 31:2313 NNP work_infoczas7nfbzac5mhjnxpkxle 442 5 - - SYM work_infoczas7nfbzac5mhjnxpkxle 442 6 2322 2322 CD work_infoczas7nfbzac5mhjnxpkxle 442 7 . . . work_infoczas7nfbzac5mhjnxpkxle 443 1 ABSTRACT ABSTRACT NNP work_infoczas7nfbzac5mhjnxpkxle 443 2 Modelling Modelling NNP work_infoczas7nfbzac5mhjnxpkxle 443 3 of of IN work_infoczas7nfbzac5mhjnxpkxle 443 4 human human JJ work_infoczas7nfbzac5mhjnxpkxle 443 5 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 443 6 in in IN work_infoczas7nfbzac5mhjnxpkxle 443 7 genetically genetically RB work_infoczas7nfbzac5mhjnxpkxle 443 8 engineered engineer VBN work_infoczas7nfbzac5mhjnxpkxle 443 9 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 443 10 provides provide VBZ work_infoczas7nfbzac5mhjnxpkxle 443 11 unique unique JJ work_infoczas7nfbzac5mhjnxpkxle 443 12 possibilities possibility NNS work_infoczas7nfbzac5mhjnxpkxle 443 13 in in IN work_infoczas7nfbzac5mhjnxpkxle 443 14 biomedical biomedical JJ work_infoczas7nfbzac5mhjnxpkxle 443 15 research research NN work_infoczas7nfbzac5mhjnxpkxle 443 16 and and CC work_infoczas7nfbzac5mhjnxpkxle 443 17 in in IN work_infoczas7nfbzac5mhjnxpkxle 443 18 studies study NNS work_infoczas7nfbzac5mhjnxpkxle 443 19 of of IN work_infoczas7nfbzac5mhjnxpkxle 443 20 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 443 21 intervention intervention NN work_infoczas7nfbzac5mhjnxpkxle 443 22 . . . work_infoczas7nfbzac5mhjnxpkxle 444 1 Establishment establishment NN work_infoczas7nfbzac5mhjnxpkxle 444 2 of of IN work_infoczas7nfbzac5mhjnxpkxle 444 3 methodologies methodology NNS work_infoczas7nfbzac5mhjnxpkxle 444 4 that that WDT work_infoczas7nfbzac5mhjnxpkxle 444 5 allow allow VBP work_infoczas7nfbzac5mhjnxpkxle 444 6 efficient efficient JJ work_infoczas7nfbzac5mhjnxpkxle 444 7 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 444 8 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 444 9 by by IN work_infoczas7nfbzac5mhjnxpkxle 444 10 non non JJ work_infoczas7nfbzac5mhjnxpkxle 444 11 - - JJ work_infoczas7nfbzac5mhjnxpkxle 444 12 viral viral JJ work_infoczas7nfbzac5mhjnxpkxle 444 13 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 444 14 carriers carrier NNS work_infoczas7nfbzac5mhjnxpkxle 444 15 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 444 16 an an DT work_infoczas7nfbzac5mhjnxpkxle 444 17 important important JJ work_infoczas7nfbzac5mhjnxpkxle 444 18 step step NN work_infoczas7nfbzac5mhjnxpkxle 444 19 towards towards IN work_infoczas7nfbzac5mhjnxpkxle 444 20 development development NN work_infoczas7nfbzac5mhjnxpkxle 444 21 of of IN work_infoczas7nfbzac5mhjnxpkxle 444 22 new new JJ work_infoczas7nfbzac5mhjnxpkxle 444 23 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 444 24 models model NNS work_infoczas7nfbzac5mhjnxpkxle 444 25 . . . work_infoczas7nfbzac5mhjnxpkxle 445 1 In in IN work_infoczas7nfbzac5mhjnxpkxle 445 2 this this DT work_infoczas7nfbzac5mhjnxpkxle 445 3 report report NN work_infoczas7nfbzac5mhjnxpkxle 445 4 , , , work_infoczas7nfbzac5mhjnxpkxle 445 5 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 445 6 present present VBP work_infoczas7nfbzac5mhjnxpkxle 445 7 transgenic transgenic JJ work_infoczas7nfbzac5mhjnxpkxle 445 8 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 445 9 created create VBN work_infoczas7nfbzac5mhjnxpkxle 445 10 by by IN work_infoczas7nfbzac5mhjnxpkxle 445 11 Sleeping sleep VBG work_infoczas7nfbzac5mhjnxpkxle 445 12 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 445 13 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 445 14 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 445 15 in in IN work_infoczas7nfbzac5mhjnxpkxle 445 16 primary primary JJ work_infoczas7nfbzac5mhjnxpkxle 445 17 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 445 18 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 445 19 in in IN work_infoczas7nfbzac5mhjnxpkxle 445 20 combination combination NN work_infoczas7nfbzac5mhjnxpkxle 445 21 with with IN work_infoczas7nfbzac5mhjnxpkxle 445 22 somatic somatic JJ work_infoczas7nfbzac5mhjnxpkxle 445 23 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 445 24 nuclear nuclear JJ work_infoczas7nfbzac5mhjnxpkxle 445 25 transfer transfer NN work_infoczas7nfbzac5mhjnxpkxle 445 26 by by IN work_infoczas7nfbzac5mhjnxpkxle 445 27 handmade handmade NN work_infoczas7nfbzac5mhjnxpkxle 445 28 cloning cloning NN work_infoczas7nfbzac5mhjnxpkxle 445 29 . . . work_infoczas7nfbzac5mhjnxpkxle 446 1 Göttingen Göttingen NNP work_infoczas7nfbzac5mhjnxpkxle 446 2 minipigs minipig NNS work_infoczas7nfbzac5mhjnxpkxle 446 3 expressing express VBG work_infoczas7nfbzac5mhjnxpkxle 446 4 green green JJ work_infoczas7nfbzac5mhjnxpkxle 446 5 fluorescent fluorescent NN work_infoczas7nfbzac5mhjnxpkxle 446 6 protein protein NN work_infoczas7nfbzac5mhjnxpkxle 446 7 are be VBP work_infoczas7nfbzac5mhjnxpkxle 446 8 produced produce VBN work_infoczas7nfbzac5mhjnxpkxle 446 9 by by IN work_infoczas7nfbzac5mhjnxpkxle 446 10 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 446 11 with with IN work_infoczas7nfbzac5mhjnxpkxle 446 12 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 446 13 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 446 14 vectors vector NNS work_infoczas7nfbzac5mhjnxpkxle 446 15 carrying carry VBG work_infoczas7nfbzac5mhjnxpkxle 446 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 446 17 transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 446 18 driven drive VBN work_infoczas7nfbzac5mhjnxpkxle 446 19 by by IN work_infoczas7nfbzac5mhjnxpkxle 446 20 the the DT work_infoczas7nfbzac5mhjnxpkxle 446 21 human human NNP work_infoczas7nfbzac5mhjnxpkxle 446 22 ubiquitin ubiquitin NNP work_infoczas7nfbzac5mhjnxpkxle 446 23 C C NNP work_infoczas7nfbzac5mhjnxpkxle 446 24 promoter promoter NN work_infoczas7nfbzac5mhjnxpkxle 446 25 . . . work_infoczas7nfbzac5mhjnxpkxle 447 1 These these DT work_infoczas7nfbzac5mhjnxpkxle 447 2 animals animal NNS work_infoczas7nfbzac5mhjnxpkxle 447 3 carry carry VBP work_infoczas7nfbzac5mhjnxpkxle 447 4 multiple multiple JJ work_infoczas7nfbzac5mhjnxpkxle 447 5 copies copy NNS work_infoczas7nfbzac5mhjnxpkxle 447 6 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 447 7 from from IN work_infoczas7nfbzac5mhjnxpkxle 447 8 8 8 CD work_infoczas7nfbzac5mhjnxpkxle 447 9 to to IN work_infoczas7nfbzac5mhjnxpkxle 447 10 13 13 CD work_infoczas7nfbzac5mhjnxpkxle 447 11 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 447 12 of of IN work_infoczas7nfbzac5mhjnxpkxle 447 13 the the DT work_infoczas7nfbzac5mhjnxpkxle 447 14 transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 447 15 and and CC work_infoczas7nfbzac5mhjnxpkxle 447 16 show show VB work_infoczas7nfbzac5mhjnxpkxle 447 17 systemic systemic JJ work_infoczas7nfbzac5mhjnxpkxle 447 18 transgene transgene JJ work_infoczas7nfbzac5mhjnxpkxle 447 19 expression expression NN work_infoczas7nfbzac5mhjnxpkxle 447 20 . . . work_infoczas7nfbzac5mhjnxpkxle 448 1 Transgene transgene NN work_infoczas7nfbzac5mhjnxpkxle 448 2 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 448 3 expressing express VBG work_infoczas7nfbzac5mhjnxpkxle 448 4 pigs pig NNS work_infoczas7nfbzac5mhjnxpkxle 448 5 carry carry VBP work_infoczas7nfbzac5mhjnxpkxle 448 6 both both DT work_infoczas7nfbzac5mhjnxpkxle 448 7 transposase transposase NN work_infoczas7nfbzac5mhjnxpkxle 448 8 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 448 9 catalyzed catalyzed JJ work_infoczas7nfbzac5mhjnxpkxle 448 10 insertions insertion NNS work_infoczas7nfbzac5mhjnxpkxle 448 11 and and CC work_infoczas7nfbzac5mhjnxpkxle 448 12 at at RB work_infoczas7nfbzac5mhjnxpkxle 448 13 least least RBS work_infoczas7nfbzac5mhjnxpkxle 448 14 one one CD work_infoczas7nfbzac5mhjnxpkxle 448 15 copy copy NN work_infoczas7nfbzac5mhjnxpkxle 448 16 of of IN work_infoczas7nfbzac5mhjnxpkxle 448 17 randomly randomly RB work_infoczas7nfbzac5mhjnxpkxle 448 18 inserted insert VBN work_infoczas7nfbzac5mhjnxpkxle 448 19 plasmid plasmid NN work_infoczas7nfbzac5mhjnxpkxle 448 20 DNA dna NN work_infoczas7nfbzac5mhjnxpkxle 448 21 . . . work_infoczas7nfbzac5mhjnxpkxle 449 1 Our -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 449 2 findings finding NNS work_infoczas7nfbzac5mhjnxpkxle 449 3 illustrate illustrate VBP work_infoczas7nfbzac5mhjnxpkxle 449 4 critical critical JJ work_infoczas7nfbzac5mhjnxpkxle 449 5 issues issue NNS work_infoczas7nfbzac5mhjnxpkxle 449 6 related relate VBN work_infoczas7nfbzac5mhjnxpkxle 449 7 to to IN work_infoczas7nfbzac5mhjnxpkxle 449 8 DNA DNA NNP work_infoczas7nfbzac5mhjnxpkxle 449 9 transposon transposon NNP work_infoczas7nfbzac5mhjnxpkxle 449 10 - - HYPH work_infoczas7nfbzac5mhjnxpkxle 449 11 directed direct VBN work_infoczas7nfbzac5mhjnxpkxle 449 12 transgenesis transgenesis NN work_infoczas7nfbzac5mhjnxpkxle 449 13 , , , work_infoczas7nfbzac5mhjnxpkxle 449 14 including include VBG work_infoczas7nfbzac5mhjnxpkxle 449 15 coincidental coincidental NNP work_infoczas7nfbzac5mhjnxpkxle 449 16 plasmid plasmid NNP work_infoczas7nfbzac5mhjnxpkxle 449 17 insertion insertion NNP work_infoczas7nfbzac5mhjnxpkxle 449 18 and and CC work_infoczas7nfbzac5mhjnxpkxle 449 19 relatively relatively RB work_infoczas7nfbzac5mhjnxpkxle 449 20 low low JJ work_infoczas7nfbzac5mhjnxpkxle 449 21 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 449 22 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 449 23 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 449 24 activity activity NN work_infoczas7nfbzac5mhjnxpkxle 449 25 in in IN work_infoczas7nfbzac5mhjnxpkxle 449 26 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 449 27 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 449 28 , , , work_infoczas7nfbzac5mhjnxpkxle 449 29 but but CC work_infoczas7nfbzac5mhjnxpkxle 449 30 also also RB work_infoczas7nfbzac5mhjnxpkxle 449 31 provide provide VB work_infoczas7nfbzac5mhjnxpkxle 449 32 a a DT work_infoczas7nfbzac5mhjnxpkxle 449 33 platform platform NN work_infoczas7nfbzac5mhjnxpkxle 449 34 for for IN work_infoczas7nfbzac5mhjnxpkxle 449 35 future future JJ work_infoczas7nfbzac5mhjnxpkxle 449 36 development development NN work_infoczas7nfbzac5mhjnxpkxle 449 37 of of IN work_infoczas7nfbzac5mhjnxpkxle 449 38 porcine porcine NN work_infoczas7nfbzac5mhjnxpkxle 449 39 disease disease NN work_infoczas7nfbzac5mhjnxpkxle 449 40 models model NNS work_infoczas7nfbzac5mhjnxpkxle 449 41 using use VBG work_infoczas7nfbzac5mhjnxpkxle 449 42 the the DT work_infoczas7nfbzac5mhjnxpkxle 449 43 Sleeping Sleeping NNP work_infoczas7nfbzac5mhjnxpkxle 449 44 Beauty Beauty NNP work_infoczas7nfbzac5mhjnxpkxle 449 45 gene gene NN work_infoczas7nfbzac5mhjnxpkxle 449 46 insertion insertion NN work_infoczas7nfbzac5mhjnxpkxle 449 47 technology technology NN work_infoczas7nfbzac5mhjnxpkxle 449 48 . . . work_infoczas7nfbzac5mhjnxpkxle 450 1 Abstract abstract JJ work_infoczas7nfbzac5mhjnxpkxle 450 2 To to IN work_infoczas7nfbzac5mhjnxpkxle 450 3 Dr. Dr. NNP work_infoczas7nfbzac5mhjnxpkxle 450 4 Bruce Bruce NNP work_infoczas7nfbzac5mhjnxpkxle 450 5 Whitelaw Whitelaw NNP work_infoczas7nfbzac5mhjnxpkxle 450 6 Editor Editor NNP work_infoczas7nfbzac5mhjnxpkxle 450 7 in in IN work_infoczas7nfbzac5mhjnxpkxle 450 8 Chief Chief NNP work_infoczas7nfbzac5mhjnxpkxle 450 9 Transgenic Transgenic NNP work_infoczas7nfbzac5mhjnxpkxle 450 10 Research Research NNP work_infoczas7nfbzac5mhjnxpkxle 450 11 Dear Dear NNP work_infoczas7nfbzac5mhjnxpkxle 450 12 Dr. Dr. NNP work_infoczas7nfbzac5mhjnxpkxle 450 13 Whitelaw Whitelaw NNP work_infoczas7nfbzac5mhjnxpkxle 450 14 We -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 450 15 appreciate appreciate VBP work_infoczas7nfbzac5mhjnxpkxle 450 16 the the DT work_infoczas7nfbzac5mhjnxpkxle 450 17 nice nice JJ work_infoczas7nfbzac5mhjnxpkxle 450 18 words word NNS work_infoczas7nfbzac5mhjnxpkxle 450 19 and and CC work_infoczas7nfbzac5mhjnxpkxle 450 20 are be VBP work_infoczas7nfbzac5mhjnxpkxle 450 21 grateful grateful JJ work_infoczas7nfbzac5mhjnxpkxle 450 22 that that IN work_infoczas7nfbzac5mhjnxpkxle 450 23 the the DT work_infoczas7nfbzac5mhjnxpkxle 450 24 manuscript manuscript NN work_infoczas7nfbzac5mhjnxpkxle 450 25 has have VBZ work_infoczas7nfbzac5mhjnxpkxle 450 26 now now RB work_infoczas7nfbzac5mhjnxpkxle 450 27 been be VBN work_infoczas7nfbzac5mhjnxpkxle 450 28 finally finally RB work_infoczas7nfbzac5mhjnxpkxle 450 29 accepted accept VBN work_infoczas7nfbzac5mhjnxpkxle 450 30 for for IN work_infoczas7nfbzac5mhjnxpkxle 450 31 publication publication NN work_infoczas7nfbzac5mhjnxpkxle 450 32 in in IN work_infoczas7nfbzac5mhjnxpkxle 450 33 Transgenic Transgenic NNP work_infoczas7nfbzac5mhjnxpkxle 450 34 Research Research NNP work_infoczas7nfbzac5mhjnxpkxle 450 35 . . . work_infoczas7nfbzac5mhjnxpkxle 451 1 We -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 451 2 have have VBP work_infoczas7nfbzac5mhjnxpkxle 451 3 considered consider VBN work_infoczas7nfbzac5mhjnxpkxle 451 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 451 5 two two CD work_infoczas7nfbzac5mhjnxpkxle 451 6 specific specific JJ work_infoczas7nfbzac5mhjnxpkxle 451 7 comments comment NNS work_infoczas7nfbzac5mhjnxpkxle 451 8 mentioned mention VBN work_infoczas7nfbzac5mhjnxpkxle 451 9 and and CC work_infoczas7nfbzac5mhjnxpkxle 451 10 have have VBP work_infoczas7nfbzac5mhjnxpkxle 451 11 revised revise VBN work_infoczas7nfbzac5mhjnxpkxle 451 12 the the DT work_infoczas7nfbzac5mhjnxpkxle 451 13 manuscript manuscript NN work_infoczas7nfbzac5mhjnxpkxle 451 14 accordingly accordingly RB work_infoczas7nfbzac5mhjnxpkxle 451 15 . . . work_infoczas7nfbzac5mhjnxpkxle 452 1 However however RB work_infoczas7nfbzac5mhjnxpkxle 452 2 , , , work_infoczas7nfbzac5mhjnxpkxle 452 3 regarding regard VBG work_infoczas7nfbzac5mhjnxpkxle 452 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 452 5 first first JJ work_infoczas7nfbzac5mhjnxpkxle 452 6 comment comment NN work_infoczas7nfbzac5mhjnxpkxle 452 7 , , , work_infoczas7nfbzac5mhjnxpkxle 452 8 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 452 9 chose choose VBD work_infoczas7nfbzac5mhjnxpkxle 452 10 not not RB work_infoczas7nfbzac5mhjnxpkxle 452 11 to to TO work_infoczas7nfbzac5mhjnxpkxle 452 12 include include VB work_infoczas7nfbzac5mhjnxpkxle 452 13 ' ' `` work_infoczas7nfbzac5mhjnxpkxle 452 14 immortalised immortalised JJ work_infoczas7nfbzac5mhjnxpkxle 452 15 ' ' '' work_infoczas7nfbzac5mhjnxpkxle 452 16 . . . work_infoczas7nfbzac5mhjnxpkxle 453 1 In in IN work_infoczas7nfbzac5mhjnxpkxle 453 2 the the DT work_infoczas7nfbzac5mhjnxpkxle 453 3 work work NN work_infoczas7nfbzac5mhjnxpkxle 453 4 by by IN work_infoczas7nfbzac5mhjnxpkxle 453 5 Clark Clark NNP work_infoczas7nfbzac5mhjnxpkxle 453 6 and and CC work_infoczas7nfbzac5mhjnxpkxle 453 7 co- co- JJ work_infoczas7nfbzac5mhjnxpkxle 453 8 workers worker NNS work_infoczas7nfbzac5mhjnxpkxle 453 9 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 453 10 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 453 11 carried carry VBN work_infoczas7nfbzac5mhjnxpkxle 453 12 out out RP work_infoczas7nfbzac5mhjnxpkxle 453 13 in in IN work_infoczas7nfbzac5mhjnxpkxle 453 14 both both DT work_infoczas7nfbzac5mhjnxpkxle 453 15 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 453 16 fetal fetal JJ work_infoczas7nfbzac5mhjnxpkxle 453 17 fibroblasts fibroblast NNS work_infoczas7nfbzac5mhjnxpkxle 453 18 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 453 19 called call VBN work_infoczas7nfbzac5mhjnxpkxle 453 20 PFFs pff NNS work_infoczas7nfbzac5mhjnxpkxle 453 21 in in IN work_infoczas7nfbzac5mhjnxpkxle 453 22 the the DT work_infoczas7nfbzac5mhjnxpkxle 453 23 paper paper NN work_infoczas7nfbzac5mhjnxpkxle 453 24 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 453 25 and and CC work_infoczas7nfbzac5mhjnxpkxle 453 26 in in IN work_infoczas7nfbzac5mhjnxpkxle 453 27 the the DT work_infoczas7nfbzac5mhjnxpkxle 453 28 immortalized immortalized JJ work_infoczas7nfbzac5mhjnxpkxle 453 29 pig pig NN work_infoczas7nfbzac5mhjnxpkxle 453 30 cell cell NN work_infoczas7nfbzac5mhjnxpkxle 453 31 line line NN work_infoczas7nfbzac5mhjnxpkxle 453 32 PEGE PEGE NNP work_infoczas7nfbzac5mhjnxpkxle 453 33 . . . work_infoczas7nfbzac5mhjnxpkxle 454 1 The the DT work_infoczas7nfbzac5mhjnxpkxle 454 2 results result NNS work_infoczas7nfbzac5mhjnxpkxle 454 3 that that WDT work_infoczas7nfbzac5mhjnxpkxle 454 4 we -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 454 5 refer refer VBP work_infoczas7nfbzac5mhjnxpkxle 454 6 to to IN work_infoczas7nfbzac5mhjnxpkxle 454 7 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 454 8 2.5-fold 2.5-fold CD work_infoczas7nfbzac5mhjnxpkxle 454 9 increase increase NN work_infoczas7nfbzac5mhjnxpkxle 454 10 in in IN work_infoczas7nfbzac5mhjnxpkxle 454 11 the the DT work_infoczas7nfbzac5mhjnxpkxle 454 12 presence presence NN work_infoczas7nfbzac5mhjnxpkxle 454 13 of of IN work_infoczas7nfbzac5mhjnxpkxle 454 14 SB11 SB11 NNP work_infoczas7nfbzac5mhjnxpkxle 454 15 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 454 16 were be VBD work_infoczas7nfbzac5mhjnxpkxle 454 17 created create VBN work_infoczas7nfbzac5mhjnxpkxle 454 18 by by IN work_infoczas7nfbzac5mhjnxpkxle 454 19 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 454 20 in in IN work_infoczas7nfbzac5mhjnxpkxle 454 21 PFFs pff NNS work_infoczas7nfbzac5mhjnxpkxle 454 22 whereas whereas IN work_infoczas7nfbzac5mhjnxpkxle 454 23 transposition transposition NN work_infoczas7nfbzac5mhjnxpkxle 454 24 in in IN work_infoczas7nfbzac5mhjnxpkxle 454 25 PEGE PEGE NNP work_infoczas7nfbzac5mhjnxpkxle 454 26 cells cell NNS work_infoczas7nfbzac5mhjnxpkxle 454 27 gave give VBD work_infoczas7nfbzac5mhjnxpkxle 454 28 a a DT work_infoczas7nfbzac5mhjnxpkxle 454 29 10-fold 10-fold JJ work_infoczas7nfbzac5mhjnxpkxle 454 30 increase increase NN work_infoczas7nfbzac5mhjnxpkxle 454 31 . . . work_infoczas7nfbzac5mhjnxpkxle 455 1 We -PRON- PRP work_infoczas7nfbzac5mhjnxpkxle 455 2 think think VBP work_infoczas7nfbzac5mhjnxpkxle 455 3 that that IN work_infoczas7nfbzac5mhjnxpkxle 455 4 the the DT work_infoczas7nfbzac5mhjnxpkxle 455 5 comparison comparison NN work_infoczas7nfbzac5mhjnxpkxle 455 6 with with IN work_infoczas7nfbzac5mhjnxpkxle 455 7 the the DT work_infoczas7nfbzac5mhjnxpkxle 455 8 data data NN work_infoczas7nfbzac5mhjnxpkxle 455 9 from from IN work_infoczas7nfbzac5mhjnxpkxle 455 10 PFF PFF NNP work_infoczas7nfbzac5mhjnxpkxle 455 11 is be VBZ work_infoczas7nfbzac5mhjnxpkxle 455 12 most most RBS work_infoczas7nfbzac5mhjnxpkxle 455 13 relevant relevant JJ work_infoczas7nfbzac5mhjnxpkxle 455 14 for for IN work_infoczas7nfbzac5mhjnxpkxle 455 15 our -PRON- PRP$ work_infoczas7nfbzac5mhjnxpkxle 455 16 study study NN work_infoczas7nfbzac5mhjnxpkxle 455 17 and and CC work_infoczas7nfbzac5mhjnxpkxle 455 18 therefore therefore RB work_infoczas7nfbzac5mhjnxpkxle 455 19 did do VBD work_infoczas7nfbzac5mhjnxpkxle 455 20 not not RB work_infoczas7nfbzac5mhjnxpkxle 455 21 introduce introduce VB work_infoczas7nfbzac5mhjnxpkxle 455 22 further further JJ work_infoczas7nfbzac5mhjnxpkxle 455 23 changes change NNS work_infoczas7nfbzac5mhjnxpkxle 455 24 in in IN work_infoczas7nfbzac5mhjnxpkxle 455 25 the the DT work_infoczas7nfbzac5mhjnxpkxle 455 26 manuscript manuscript NN work_infoczas7nfbzac5mhjnxpkxle 455 27 . . . work_infoczas7nfbzac5mhjnxpkxle 456 1 Sincerely sincerely RB work_infoczas7nfbzac5mhjnxpkxle 456 2 , , , work_infoczas7nfbzac5mhjnxpkxle 456 3 Jacob Jacob NNP work_infoczas7nfbzac5mhjnxpkxle 456 4 Giehm Giehm NNP work_infoczas7nfbzac5mhjnxpkxle 456 5 Mikkelsen Mikkelsen NNP work_infoczas7nfbzac5mhjnxpkxle 456 6 , , , work_infoczas7nfbzac5mhjnxpkxle 456 7 PhD phd NN work_infoczas7nfbzac5mhjnxpkxle 456 8 Corresponding correspond VBG work_infoczas7nfbzac5mhjnxpkxle 456 9 author author NN work_infoczas7nfbzac5mhjnxpkxle 456 10 * * NFP work_infoczas7nfbzac5mhjnxpkxle 456 11 Response response NN work_infoczas7nfbzac5mhjnxpkxle 456 12 to to IN work_infoczas7nfbzac5mhjnxpkxle 456 13 reviewer reviewer NNP work_infoczas7nfbzac5mhjnxpkxle 456 14 's 's POS work_infoczas7nfbzac5mhjnxpkxle 456 15 comments comment NNS work_infoczas7nfbzac5mhjnxpkxle 456 16 Colour Colour NNP work_infoczas7nfbzac5mhjnxpkxle 456 17 figure figure NN work_infoczas7nfbzac5mhjnxpkxle 456 18 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 456 19 Figure figure NN work_infoczas7nfbzac5mhjnxpkxle 456 20 1 1 CD work_infoczas7nfbzac5mhjnxpkxle 456 21 ) ) -RRB- work_infoczas7nfbzac5mhjnxpkxle 456 22 Click click VB work_infoczas7nfbzac5mhjnxpkxle 456 23 here here RB work_infoczas7nfbzac5mhjnxpkxle 456 24 to to TO work_infoczas7nfbzac5mhjnxpkxle 456 25 download download VB work_infoczas7nfbzac5mhjnxpkxle 456 26 high high JJ work_infoczas7nfbzac5mhjnxpkxle 456 27 resolution resolution NN work_infoczas7nfbzac5mhjnxpkxle 456 28 image image NN work_infoczas7nfbzac5mhjnxpkxle 456 29 http://www.editorialmanager.com/trag/download.aspx?id=25113&guid=856d53c8-db89-43d5-81fb-564d42ec769c&scheme=1 http://www.editorialmanager.com/trag/download.aspx?id=25113&guid=856d53c8-db89-43d5-81fb-564d42ec769c&scheme=1 NNP work_infoczas7nfbzac5mhjnxpkxle 456 30 Colour colour NN work_infoczas7nfbzac5mhjnxpkxle 456 31 figure figure NN work_infoczas7nfbzac5mhjnxpkxle 456 32 ( ( -LRB- work_infoczas7nfbzac5mhjnxpkxle 456 33 Figure figure NN work_infoczas7nfbzac5mhjnxpkxle 456 34 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456 64 Click click VB work_infoczas7nfbzac5mhjnxpkxle 456 65 here here RB work_infoczas7nfbzac5mhjnxpkxle 456 66 to to TO work_infoczas7nfbzac5mhjnxpkxle 456 67 download download VB work_infoczas7nfbzac5mhjnxpkxle 456 68 high high JJ work_infoczas7nfbzac5mhjnxpkxle 456 69 resolution resolution NN work_infoczas7nfbzac5mhjnxpkxle 456 70 image image NN work_infoczas7nfbzac5mhjnxpkxle 456 71 http://www.editorialmanager.com/trag/download.aspx?id=25116&guid=571a9abe-d4b1-43a9-869b-b1685a686eaf&scheme=1 http://www.editorialmanager.com/trag/download.aspx?id=25116&guid=571a9abe-d4b1-43a9-869b-b1685a686eaf&scheme=1 .