id sid tid token lemma pos work_sny56posuffadh5a367n5vi4uq 1 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 1 2 of of IN work_sny56posuffadh5a367n5vi4uq 1 3 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 1 4 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 1 5 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 1 6 : : : work_sny56posuffadh5a367n5vi4uq 1 7 parameters parameter NNS work_sny56posuffadh5a367n5vi4uq 1 8 and and CC work_sny56posuffadh5a367n5vi4uq 1 9 applications application NNS work_sny56posuffadh5a367n5vi4uq 1 10 to to TO work_sny56posuffadh5a367n5vi4uq 1 11 gene gene VB work_sny56posuffadh5a367n5vi4uq 1 12 therapy therapy NN work_sny56posuffadh5a367n5vi4uq 1 13 THE the DT work_sny56posuffadh5a367n5vi4uq 1 14 JOURNAL JOURNAL NNP work_sny56posuffadh5a367n5vi4uq 1 15 OF of IN work_sny56posuffadh5a367n5vi4uq 1 16 GENE gene NN work_sny56posuffadh5a367n5vi4uq 1 17 MEDICINE medicine NN work_sny56posuffadh5a367n5vi4uq 1 18 R r NN work_sny56posuffadh5a367n5vi4uq 1 19 E e NN work_sny56posuffadh5a367n5vi4uq 1 20 S s NN work_sny56posuffadh5a367n5vi4uq 1 21 E e NN work_sny56posuffadh5a367n5vi4uq 1 22 A a NN work_sny56posuffadh5a367n5vi4uq 1 23 R r NN work_sny56posuffadh5a367n5vi4uq 1 24 C c NN work_sny56posuffadh5a367n5vi4uq 1 25 H h NN work_sny56posuffadh5a367n5vi4uq 1 26 A a NN work_sny56posuffadh5a367n5vi4uq 1 27 R r NN work_sny56posuffadh5a367n5vi4uq 1 28 T t NN work_sny56posuffadh5a367n5vi4uq 1 29 I i NN work_sny56posuffadh5a367n5vi4uq 1 30 C c NN work_sny56posuffadh5a367n5vi4uq 1 31 L l NN work_sny56posuffadh5a367n5vi4uq 1 32 E e NN work_sny56posuffadh5a367n5vi4uq 1 33 J j NN work_sny56posuffadh5a367n5vi4uq 1 34 Gene Gene NNP work_sny56posuffadh5a367n5vi4uq 1 35 Med Med NNP work_sny56posuffadh5a367n5vi4uq 1 36 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 1 37 ; ; : work_sny56posuffadh5a367n5vi4uq 1 38 6 6 CD work_sny56posuffadh5a367n5vi4uq 1 39 : : : work_sny56posuffadh5a367n5vi4uq 1 40 574 574 CD work_sny56posuffadh5a367n5vi4uq 1 41 – – : work_sny56posuffadh5a367n5vi4uq 1 42 583 583 CD work_sny56posuffadh5a367n5vi4uq 1 43 . . . work_sny56posuffadh5a367n5vi4uq 2 1 Published publish VBN work_sny56posuffadh5a367n5vi4uq 2 2 online online JJ work_sny56posuffadh5a367n5vi4uq 2 3 8 8 CD work_sny56posuffadh5a367n5vi4uq 2 4 March March NNP work_sny56posuffadh5a367n5vi4uq 2 5 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 2 6 in in IN work_sny56posuffadh5a367n5vi4uq 2 7 Wiley Wiley NNP work_sny56posuffadh5a367n5vi4uq 2 8 InterScience InterScience NNP work_sny56posuffadh5a367n5vi4uq 2 9 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 2 10 www.interscience.wiley.com www.interscience.wiley.com CD work_sny56posuffadh5a367n5vi4uq 2 11 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 2 12 . . . work_sny56posuffadh5a367n5vi4uq 3 1 DOI doi NN work_sny56posuffadh5a367n5vi4uq 3 2 : : : work_sny56posuffadh5a367n5vi4uq 3 3 10.1002 10.1002 CD work_sny56posuffadh5a367n5vi4uq 3 4 / / SYM work_sny56posuffadh5a367n5vi4uq 3 5 jgm.486 jgm.486 NNP work_sny56posuffadh5a367n5vi4uq 3 6 Excision excision NN work_sny56posuffadh5a367n5vi4uq 3 7 of of IN work_sny56posuffadh5a367n5vi4uq 3 8 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 3 9 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 3 10 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 3 11 : : : work_sny56posuffadh5a367n5vi4uq 3 12 parameters parameter NNS work_sny56posuffadh5a367n5vi4uq 3 13 and and CC work_sny56posuffadh5a367n5vi4uq 3 14 applications application NNS work_sny56posuffadh5a367n5vi4uq 3 15 to to TO work_sny56posuffadh5a367n5vi4uq 3 16 gene gene VB work_sny56posuffadh5a367n5vi4uq 3 17 therapy therapy NN work_sny56posuffadh5a367n5vi4uq 3 18 Geyi Geyi NNP work_sny56posuffadh5a367n5vi4uq 3 19 Liu1,2 Liu1,2 NNP work_sny56posuffadh5a367n5vi4uq 3 20 Elena Elena NNP work_sny56posuffadh5a367n5vi4uq 3 21 L. L. NNP work_sny56posuffadh5a367n5vi4uq 3 22 Aronovich1,3 aronovich1,3 CD work_sny56posuffadh5a367n5vi4uq 3 23 Zongbin Zongbin NNP work_sny56posuffadh5a367n5vi4uq 3 24 Cui1,2,4 Cui1,2,4 VBG work_sny56posuffadh5a367n5vi4uq 3 25 Chester Chester NNP work_sny56posuffadh5a367n5vi4uq 3 26 B. B. NNP work_sny56posuffadh5a367n5vi4uq 3 27 Whitley1,3 Whitley1,3 NNP work_sny56posuffadh5a367n5vi4uq 3 28 Perry Perry NNP work_sny56posuffadh5a367n5vi4uq 3 29 B. B. NNP work_sny56posuffadh5a367n5vi4uq 3 30 Hackett1,2,5 Hackett1,2,5 NNP work_sny56posuffadh5a367n5vi4uq 3 31 * * NFP work_sny56posuffadh5a367n5vi4uq 3 32 1Department 1department CD work_sny56posuffadh5a367n5vi4uq 3 33 of of IN work_sny56posuffadh5a367n5vi4uq 3 34 Genetics Genetics NNP work_sny56posuffadh5a367n5vi4uq 3 35 , , , work_sny56posuffadh5a367n5vi4uq 3 36 Cell Cell NNP work_sny56posuffadh5a367n5vi4uq 3 37 Biology Biology NNP work_sny56posuffadh5a367n5vi4uq 3 38 and and CC work_sny56posuffadh5a367n5vi4uq 3 39 Development Development NNP work_sny56posuffadh5a367n5vi4uq 3 40 and and CC work_sny56posuffadh5a367n5vi4uq 3 41 The the DT work_sny56posuffadh5a367n5vi4uq 3 42 Institute Institute NNP work_sny56posuffadh5a367n5vi4uq 3 43 of of IN work_sny56posuffadh5a367n5vi4uq 3 44 Human Human NNP work_sny56posuffadh5a367n5vi4uq 3 45 Genetics Genetics NNP work_sny56posuffadh5a367n5vi4uq 3 46 , , , work_sny56posuffadh5a367n5vi4uq 3 47 University University NNP work_sny56posuffadh5a367n5vi4uq 3 48 of of IN work_sny56posuffadh5a367n5vi4uq 3 49 Minnesota Minnesota NNP work_sny56posuffadh5a367n5vi4uq 3 50 , , , work_sny56posuffadh5a367n5vi4uq 3 51 Minneapolis Minneapolis NNP work_sny56posuffadh5a367n5vi4uq 3 52 , , , work_sny56posuffadh5a367n5vi4uq 3 53 MN MN NNP work_sny56posuffadh5a367n5vi4uq 3 54 55455 55455 CD work_sny56posuffadh5a367n5vi4uq 3 55 , , , work_sny56posuffadh5a367n5vi4uq 3 56 USA USA NNP work_sny56posuffadh5a367n5vi4uq 3 57 2The 2the CD work_sny56posuffadh5a367n5vi4uq 3 58 Arnold Arnold NNP work_sny56posuffadh5a367n5vi4uq 3 59 and and CC work_sny56posuffadh5a367n5vi4uq 3 60 Mabel Mabel NNP work_sny56posuffadh5a367n5vi4uq 3 61 Beckman Beckman NNP work_sny56posuffadh5a367n5vi4uq 3 62 Center Center NNP work_sny56posuffadh5a367n5vi4uq 3 63 for for IN work_sny56posuffadh5a367n5vi4uq 3 64 Transposon Transposon NNP work_sny56posuffadh5a367n5vi4uq 3 65 Research Research NNP work_sny56posuffadh5a367n5vi4uq 3 66 , , , work_sny56posuffadh5a367n5vi4uq 3 67 University University NNP work_sny56posuffadh5a367n5vi4uq 3 68 of of IN work_sny56posuffadh5a367n5vi4uq 3 69 Minnesota Minnesota NNP work_sny56posuffadh5a367n5vi4uq 3 70 , , , work_sny56posuffadh5a367n5vi4uq 3 71 Minneapolis Minneapolis NNP work_sny56posuffadh5a367n5vi4uq 3 72 , , , work_sny56posuffadh5a367n5vi4uq 3 73 MN MN NNP work_sny56posuffadh5a367n5vi4uq 3 74 55455 55455 CD work_sny56posuffadh5a367n5vi4uq 3 75 , , , work_sny56posuffadh5a367n5vi4uq 3 76 USA USA NNP work_sny56posuffadh5a367n5vi4uq 3 77 3Department 3Department NNP work_sny56posuffadh5a367n5vi4uq 3 78 of of IN work_sny56posuffadh5a367n5vi4uq 3 79 Pediatrics Pediatrics NNPS work_sny56posuffadh5a367n5vi4uq 3 80 , , , work_sny56posuffadh5a367n5vi4uq 3 81 University University NNP work_sny56posuffadh5a367n5vi4uq 3 82 of of IN work_sny56posuffadh5a367n5vi4uq 3 83 Minnesota Minnesota NNP work_sny56posuffadh5a367n5vi4uq 3 84 , , , work_sny56posuffadh5a367n5vi4uq 3 85 Minneapolis Minneapolis NNP work_sny56posuffadh5a367n5vi4uq 3 86 , , , work_sny56posuffadh5a367n5vi4uq 3 87 MN MN NNP work_sny56posuffadh5a367n5vi4uq 3 88 55455 55455 CD work_sny56posuffadh5a367n5vi4uq 3 89 , , , work_sny56posuffadh5a367n5vi4uq 3 90 USA USA NNP work_sny56posuffadh5a367n5vi4uq 3 91 4Institite 4Institite NNP work_sny56posuffadh5a367n5vi4uq 3 92 of of IN work_sny56posuffadh5a367n5vi4uq 3 93 Hydrobiology Hydrobiology NNP work_sny56posuffadh5a367n5vi4uq 3 94 , , , work_sny56posuffadh5a367n5vi4uq 3 95 Chinese Chinese NNP work_sny56posuffadh5a367n5vi4uq 3 96 Academy Academy NNP work_sny56posuffadh5a367n5vi4uq 3 97 of of IN work_sny56posuffadh5a367n5vi4uq 3 98 Sciences Sciences NNPS work_sny56posuffadh5a367n5vi4uq 3 99 , , , work_sny56posuffadh5a367n5vi4uq 3 100 Wuhan Wuhan NNP work_sny56posuffadh5a367n5vi4uq 3 101 , , , work_sny56posuffadh5a367n5vi4uq 3 102 P.R. P.R. NNP work_sny56posuffadh5a367n5vi4uq 4 1 China China NNP work_sny56posuffadh5a367n5vi4uq 4 2 5Discovery 5Discovery NNP work_sny56posuffadh5a367n5vi4uq 4 3 Genomics Genomics NNP work_sny56posuffadh5a367n5vi4uq 4 4 , , , work_sny56posuffadh5a367n5vi4uq 4 5 Inc. Inc. NNP work_sny56posuffadh5a367n5vi4uq 4 6 , , , work_sny56posuffadh5a367n5vi4uq 4 7 Minneapolis Minneapolis NNP work_sny56posuffadh5a367n5vi4uq 4 8 , , , work_sny56posuffadh5a367n5vi4uq 4 9 MN MN NNP work_sny56posuffadh5a367n5vi4uq 4 10 55413 55413 CD work_sny56posuffadh5a367n5vi4uq 4 11 , , , work_sny56posuffadh5a367n5vi4uq 4 12 USA USA NNP work_sny56posuffadh5a367n5vi4uq 4 13 * * NFP work_sny56posuffadh5a367n5vi4uq 4 14 Correspondence correspondence NN work_sny56posuffadh5a367n5vi4uq 4 15 to to TO work_sny56posuffadh5a367n5vi4uq 4 16 : : : work_sny56posuffadh5a367n5vi4uq 4 17 Perry Perry NNP work_sny56posuffadh5a367n5vi4uq 4 18 B. B. NNP work_sny56posuffadh5a367n5vi4uq 4 19 Hackett Hackett NNP work_sny56posuffadh5a367n5vi4uq 4 20 , , , work_sny56posuffadh5a367n5vi4uq 4 21 Discovery Discovery NNP work_sny56posuffadh5a367n5vi4uq 4 22 Genomics Genomics NNP work_sny56posuffadh5a367n5vi4uq 4 23 , , , work_sny56posuffadh5a367n5vi4uq 4 24 Inc. Inc. NNP work_sny56posuffadh5a367n5vi4uq 4 25 , , , work_sny56posuffadh5a367n5vi4uq 4 26 614 614 CD work_sny56posuffadh5a367n5vi4uq 4 27 McKinley McKinley NNP work_sny56posuffadh5a367n5vi4uq 4 28 Place Place NNP work_sny56posuffadh5a367n5vi4uq 4 29 NE NE NNP work_sny56posuffadh5a367n5vi4uq 4 30 , , , work_sny56posuffadh5a367n5vi4uq 4 31 Minneapolis Minneapolis NNP work_sny56posuffadh5a367n5vi4uq 4 32 , , , work_sny56posuffadh5a367n5vi4uq 4 33 MN MN NNP work_sny56posuffadh5a367n5vi4uq 4 34 55413 55413 CD work_sny56posuffadh5a367n5vi4uq 4 35 , , , work_sny56posuffadh5a367n5vi4uq 4 36 USA USA NNP work_sny56posuffadh5a367n5vi4uq 4 37 . . . work_sny56posuffadh5a367n5vi4uq 5 1 E e NN work_sny56posuffadh5a367n5vi4uq 5 2 - - NN work_sny56posuffadh5a367n5vi4uq 5 3 mail mail NN work_sny56posuffadh5a367n5vi4uq 5 4 : : : work_sny56posuffadh5a367n5vi4uq 5 5 perryh@discoverygenomics.net perryh@discoverygenomics.net XX work_sny56posuffadh5a367n5vi4uq 5 6 Received receive VBN work_sny56posuffadh5a367n5vi4uq 5 7 : : : work_sny56posuffadh5a367n5vi4uq 5 8 13 13 CD work_sny56posuffadh5a367n5vi4uq 5 9 March March NNP work_sny56posuffadh5a367n5vi4uq 5 10 2003 2003 CD work_sny56posuffadh5a367n5vi4uq 5 11 Revised revise VBN work_sny56posuffadh5a367n5vi4uq 5 12 : : : work_sny56posuffadh5a367n5vi4uq 5 13 27 27 CD work_sny56posuffadh5a367n5vi4uq 5 14 May May NNP work_sny56posuffadh5a367n5vi4uq 5 15 2003 2003 CD work_sny56posuffadh5a367n5vi4uq 5 16 Accepted accept VBN work_sny56posuffadh5a367n5vi4uq 5 17 : : : work_sny56posuffadh5a367n5vi4uq 5 18 27 27 CD work_sny56posuffadh5a367n5vi4uq 5 19 June June NNP work_sny56posuffadh5a367n5vi4uq 5 20 2003 2003 CD work_sny56posuffadh5a367n5vi4uq 5 21 Abstract Abstract NNP work_sny56posuffadh5a367n5vi4uq 5 22 A a DT work_sny56posuffadh5a367n5vi4uq 5 23 major major JJ work_sny56posuffadh5a367n5vi4uq 5 24 problem problem NN work_sny56posuffadh5a367n5vi4uq 5 25 in in IN work_sny56posuffadh5a367n5vi4uq 5 26 gene gene NN work_sny56posuffadh5a367n5vi4uq 5 27 therapy therapy NN work_sny56posuffadh5a367n5vi4uq 5 28 is be VBZ work_sny56posuffadh5a367n5vi4uq 5 29 the the DT work_sny56posuffadh5a367n5vi4uq 5 30 determination determination NN work_sny56posuffadh5a367n5vi4uq 5 31 of of IN work_sny56posuffadh5a367n5vi4uq 5 32 the the DT work_sny56posuffadh5a367n5vi4uq 5 33 rates rate NNS work_sny56posuffadh5a367n5vi4uq 5 34 at at IN work_sny56posuffadh5a367n5vi4uq 5 35 which which WDT work_sny56posuffadh5a367n5vi4uq 5 36 gene gene NN work_sny56posuffadh5a367n5vi4uq 5 37 transfer transfer NN work_sny56posuffadh5a367n5vi4uq 5 38 has have VBZ work_sny56posuffadh5a367n5vi4uq 5 39 occurred occur VBN work_sny56posuffadh5a367n5vi4uq 5 40 . . . work_sny56posuffadh5a367n5vi4uq 6 1 Our -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 6 2 work work NN work_sny56posuffadh5a367n5vi4uq 6 3 has have VBZ work_sny56posuffadh5a367n5vi4uq 6 4 focused focus VBN work_sny56posuffadh5a367n5vi4uq 6 5 on on IN work_sny56posuffadh5a367n5vi4uq 6 6 applications application NNS work_sny56posuffadh5a367n5vi4uq 6 7 of of IN work_sny56posuffadh5a367n5vi4uq 6 8 the the DT work_sny56posuffadh5a367n5vi4uq 6 9 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 6 10 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 6 11 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 6 12 SB SB NNP work_sny56posuffadh5a367n5vi4uq 6 13 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 6 14 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 6 15 system system NN work_sny56posuffadh5a367n5vi4uq 6 16 as as IN work_sny56posuffadh5a367n5vi4uq 6 17 a a DT work_sny56posuffadh5a367n5vi4uq 6 18 non non JJ work_sny56posuffadh5a367n5vi4uq 6 19 - - JJ work_sny56posuffadh5a367n5vi4uq 6 20 viral viral JJ work_sny56posuffadh5a367n5vi4uq 6 21 vector vector NN work_sny56posuffadh5a367n5vi4uq 6 22 for for IN work_sny56posuffadh5a367n5vi4uq 6 23 gene gene NN work_sny56posuffadh5a367n5vi4uq 6 24 therapy therapy NN work_sny56posuffadh5a367n5vi4uq 6 25 . . . work_sny56posuffadh5a367n5vi4uq 7 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 7 2 of of IN work_sny56posuffadh5a367n5vi4uq 7 3 a a DT work_sny56posuffadh5a367n5vi4uq 7 4 transposon transposon NN work_sny56posuffadh5a367n5vi4uq 7 5 from from IN work_sny56posuffadh5a367n5vi4uq 7 6 a a DT work_sny56posuffadh5a367n5vi4uq 7 7 donor donor NN work_sny56posuffadh5a367n5vi4uq 7 8 molecule molecule NN work_sny56posuffadh5a367n5vi4uq 7 9 and and CC work_sny56posuffadh5a367n5vi4uq 7 10 its -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 7 11 integration integration NN work_sny56posuffadh5a367n5vi4uq 7 12 into into IN work_sny56posuffadh5a367n5vi4uq 7 13 a a DT work_sny56posuffadh5a367n5vi4uq 7 14 cellular cellular JJ work_sny56posuffadh5a367n5vi4uq 7 15 chromosome chromosome NN work_sny56posuffadh5a367n5vi4uq 7 16 are be VBP work_sny56posuffadh5a367n5vi4uq 7 17 catalyzed catalyze VBN work_sny56posuffadh5a367n5vi4uq 7 18 by by IN work_sny56posuffadh5a367n5vi4uq 7 19 SB SB NNP work_sny56posuffadh5a367n5vi4uq 7 20 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 7 21 . . . work_sny56posuffadh5a367n5vi4uq 8 1 In in IN work_sny56posuffadh5a367n5vi4uq 8 2 this this DT work_sny56posuffadh5a367n5vi4uq 8 3 study study NN work_sny56posuffadh5a367n5vi4uq 8 4 , , , work_sny56posuffadh5a367n5vi4uq 8 5 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 8 6 used use VBD work_sny56posuffadh5a367n5vi4uq 8 7 a a DT work_sny56posuffadh5a367n5vi4uq 8 8 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 8 9 - - HYPH work_sny56posuffadh5a367n5vi4uq 8 10 based base VBN work_sny56posuffadh5a367n5vi4uq 8 11 excision excision NN work_sny56posuffadh5a367n5vi4uq 8 12 assay assay NN work_sny56posuffadh5a367n5vi4uq 8 13 to to TO work_sny56posuffadh5a367n5vi4uq 8 14 study study VB work_sny56posuffadh5a367n5vi4uq 8 15 the the DT work_sny56posuffadh5a367n5vi4uq 8 16 excision excision NN work_sny56posuffadh5a367n5vi4uq 8 17 step step NN work_sny56posuffadh5a367n5vi4uq 8 18 of of IN work_sny56posuffadh5a367n5vi4uq 8 19 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 8 20 . . . work_sny56posuffadh5a367n5vi4uq 9 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 9 2 used use VBD work_sny56posuffadh5a367n5vi4uq 9 3 the the DT work_sny56posuffadh5a367n5vi4uq 9 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 9 5 assay assay NN work_sny56posuffadh5a367n5vi4uq 9 6 to to TO work_sny56posuffadh5a367n5vi4uq 9 7 evaluate evaluate VB work_sny56posuffadh5a367n5vi4uq 9 8 the the DT work_sny56posuffadh5a367n5vi4uq 9 9 importance importance NN work_sny56posuffadh5a367n5vi4uq 9 10 of of IN work_sny56posuffadh5a367n5vi4uq 9 11 various various JJ work_sny56posuffadh5a367n5vi4uq 9 12 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 9 13 that that WDT work_sny56posuffadh5a367n5vi4uq 9 14 border border VBP work_sny56posuffadh5a367n5vi4uq 9 15 the the DT work_sny56posuffadh5a367n5vi4uq 9 16 sites site NNS work_sny56posuffadh5a367n5vi4uq 9 17 of of IN work_sny56posuffadh5a367n5vi4uq 9 18 excision excision NN work_sny56posuffadh5a367n5vi4uq 9 19 inside inside IN work_sny56posuffadh5a367n5vi4uq 9 20 and and CC work_sny56posuffadh5a367n5vi4uq 9 21 outside outside IN work_sny56posuffadh5a367n5vi4uq 9 22 the the DT work_sny56posuffadh5a367n5vi4uq 9 23 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 9 24 in in IN work_sny56posuffadh5a367n5vi4uq 9 25 order order NN work_sny56posuffadh5a367n5vi4uq 9 26 to to TO work_sny56posuffadh5a367n5vi4uq 9 27 determine determine VB work_sny56posuffadh5a367n5vi4uq 9 28 the the DT work_sny56posuffadh5a367n5vi4uq 9 29 most most RBS work_sny56posuffadh5a367n5vi4uq 9 30 active active JJ work_sny56posuffadh5a367n5vi4uq 9 31 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 9 32 for for IN work_sny56posuffadh5a367n5vi4uq 9 33 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 9 34 from from IN work_sny56posuffadh5a367n5vi4uq 9 35 a a DT work_sny56posuffadh5a367n5vi4uq 9 36 donor donor NN work_sny56posuffadh5a367n5vi4uq 9 37 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 9 38 . . . work_sny56posuffadh5a367n5vi4uq 10 1 These these DT work_sny56posuffadh5a367n5vi4uq 10 2 findings finding NNS work_sny56posuffadh5a367n5vi4uq 10 3 together together RB work_sny56posuffadh5a367n5vi4uq 10 4 with with IN work_sny56posuffadh5a367n5vi4uq 10 5 our -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 10 6 previous previous JJ work_sny56posuffadh5a367n5vi4uq 10 7 results result NNS work_sny56posuffadh5a367n5vi4uq 10 8 in in IN work_sny56posuffadh5a367n5vi4uq 10 9 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 10 10 binding bind VBG work_sny56posuffadh5a367n5vi4uq 10 11 to to IN work_sny56posuffadh5a367n5vi4uq 10 12 the the DT work_sny56posuffadh5a367n5vi4uq 10 13 terminal terminal JJ work_sny56posuffadh5a367n5vi4uq 10 14 repeats repeat NNS work_sny56posuffadh5a367n5vi4uq 10 15 suggest suggest VBP work_sny56posuffadh5a367n5vi4uq 10 16 that that IN work_sny56posuffadh5a367n5vi4uq 10 17 the the DT work_sny56posuffadh5a367n5vi4uq 10 18 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 10 19 in in IN work_sny56posuffadh5a367n5vi4uq 10 20 the the DT work_sny56posuffadh5a367n5vi4uq 10 21 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 10 22 - - HYPH work_sny56posuffadh5a367n5vi4uq 10 23 junction junction NNP work_sny56posuffadh5a367n5vi4uq 10 24 of of IN work_sny56posuffadh5a367n5vi4uq 10 25 SB SB NNP work_sny56posuffadh5a367n5vi4uq 10 26 are be VBP work_sny56posuffadh5a367n5vi4uq 10 27 involved involve VBN work_sny56posuffadh5a367n5vi4uq 10 28 in in IN work_sny56posuffadh5a367n5vi4uq 10 29 steps step NNS work_sny56posuffadh5a367n5vi4uq 10 30 subsequent subsequent JJ work_sny56posuffadh5a367n5vi4uq 10 31 to to IN work_sny56posuffadh5a367n5vi4uq 10 32 DNA dna NN work_sny56posuffadh5a367n5vi4uq 10 33 binding binding JJ work_sny56posuffadh5a367n5vi4uq 10 34 but but CC work_sny56posuffadh5a367n5vi4uq 10 35 before before IN work_sny56posuffadh5a367n5vi4uq 10 36 excision excision NN work_sny56posuffadh5a367n5vi4uq 10 37 , , , work_sny56posuffadh5a367n5vi4uq 10 38 and and CC work_sny56posuffadh5a367n5vi4uq 10 39 that that IN work_sny56posuffadh5a367n5vi4uq 10 40 they -PRON- PRP work_sny56posuffadh5a367n5vi4uq 10 41 may may MD work_sny56posuffadh5a367n5vi4uq 10 42 have have VB work_sny56posuffadh5a367n5vi4uq 10 43 a a DT work_sny56posuffadh5a367n5vi4uq 10 44 role role NN work_sny56posuffadh5a367n5vi4uq 10 45 in in IN work_sny56posuffadh5a367n5vi4uq 10 46 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 10 47 – – : work_sny56posuffadh5a367n5vi4uq 10 48 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 10 49 interaction interaction NN work_sny56posuffadh5a367n5vi4uq 10 50 . . . work_sny56posuffadh5a367n5vi4uq 11 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 11 2 found find VBD work_sny56posuffadh5a367n5vi4uq 11 3 that that IN work_sny56posuffadh5a367n5vi4uq 11 4 SB SB NNP work_sny56posuffadh5a367n5vi4uq 11 5 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 11 6 leave leave VBP work_sny56posuffadh5a367n5vi4uq 11 7 characteristically characteristically RB work_sny56posuffadh5a367n5vi4uq 11 8 different different JJ work_sny56posuffadh5a367n5vi4uq 11 9 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 11 10 at at IN work_sny56posuffadh5a367n5vi4uq 11 11 excision excision NN work_sny56posuffadh5a367n5vi4uq 11 12 sites site NNS work_sny56posuffadh5a367n5vi4uq 11 13 in in IN work_sny56posuffadh5a367n5vi4uq 11 14 different different JJ work_sny56posuffadh5a367n5vi4uq 11 15 cell cell NN work_sny56posuffadh5a367n5vi4uq 11 16 types type NNS work_sny56posuffadh5a367n5vi4uq 11 17 , , , work_sny56posuffadh5a367n5vi4uq 11 18 suggesting suggest VBG work_sny56posuffadh5a367n5vi4uq 11 19 that that IN work_sny56posuffadh5a367n5vi4uq 11 20 alternative alternative JJ work_sny56posuffadh5a367n5vi4uq 11 21 repair repair NN work_sny56posuffadh5a367n5vi4uq 11 22 machineries machinery NNS work_sny56posuffadh5a367n5vi4uq 11 23 operate operate VBP work_sny56posuffadh5a367n5vi4uq 11 24 in in IN work_sny56posuffadh5a367n5vi4uq 11 25 concert concert NN work_sny56posuffadh5a367n5vi4uq 11 26 with with IN work_sny56posuffadh5a367n5vi4uq 11 27 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 11 28 . . . work_sny56posuffadh5a367n5vi4uq 12 1 Most most RBS work_sny56posuffadh5a367n5vi4uq 12 2 importantly importantly RB work_sny56posuffadh5a367n5vi4uq 12 3 , , , work_sny56posuffadh5a367n5vi4uq 12 4 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 12 5 found find VBD work_sny56posuffadh5a367n5vi4uq 12 6 that that IN work_sny56posuffadh5a367n5vi4uq 12 7 the the DT work_sny56posuffadh5a367n5vi4uq 12 8 rates rate NNS work_sny56posuffadh5a367n5vi4uq 12 9 of of IN work_sny56posuffadh5a367n5vi4uq 12 10 excision excision NN work_sny56posuffadh5a367n5vi4uq 12 11 correlate correlate VBP work_sny56posuffadh5a367n5vi4uq 12 12 with with IN work_sny56posuffadh5a367n5vi4uq 12 13 the the DT work_sny56posuffadh5a367n5vi4uq 12 14 rates rate NNS work_sny56posuffadh5a367n5vi4uq 12 15 of of IN work_sny56posuffadh5a367n5vi4uq 12 16 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 12 17 . . . work_sny56posuffadh5a367n5vi4uq 13 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 13 2 used use VBD work_sny56posuffadh5a367n5vi4uq 13 3 this this DT work_sny56posuffadh5a367n5vi4uq 13 4 finding finding NN work_sny56posuffadh5a367n5vi4uq 13 5 to to TO work_sny56posuffadh5a367n5vi4uq 13 6 assess assess VB work_sny56posuffadh5a367n5vi4uq 13 7 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 13 8 in in IN work_sny56posuffadh5a367n5vi4uq 13 9 livers liver NNS work_sny56posuffadh5a367n5vi4uq 13 10 of of IN work_sny56posuffadh5a367n5vi4uq 13 11 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 13 12 that that WDT work_sny56posuffadh5a367n5vi4uq 13 13 were be VBD work_sny56posuffadh5a367n5vi4uq 13 14 injected inject VBN work_sny56posuffadh5a367n5vi4uq 13 15 with with IN work_sny56posuffadh5a367n5vi4uq 13 16 the the DT work_sny56posuffadh5a367n5vi4uq 13 17 SB SB NNP work_sny56posuffadh5a367n5vi4uq 13 18 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 13 19 and and CC work_sny56posuffadh5a367n5vi4uq 13 20 transposase transposase NNP work_sny56posuffadh5a367n5vi4uq 13 21 . . . work_sny56posuffadh5a367n5vi4uq 14 1 The the DT work_sny56posuffadh5a367n5vi4uq 14 2 excision excision NN work_sny56posuffadh5a367n5vi4uq 14 3 assay assay NN work_sny56posuffadh5a367n5vi4uq 14 4 appears appear VBZ work_sny56posuffadh5a367n5vi4uq 14 5 to to TO work_sny56posuffadh5a367n5vi4uq 14 6 be be VB work_sny56posuffadh5a367n5vi4uq 14 7 a a DT work_sny56posuffadh5a367n5vi4uq 14 8 relatively relatively RB work_sny56posuffadh5a367n5vi4uq 14 9 quick quick JJ work_sny56posuffadh5a367n5vi4uq 14 10 and and CC work_sny56posuffadh5a367n5vi4uq 14 11 easy easy JJ work_sny56posuffadh5a367n5vi4uq 14 12 method method NN work_sny56posuffadh5a367n5vi4uq 14 13 to to TO work_sny56posuffadh5a367n5vi4uq 14 14 optimize optimize VB work_sny56posuffadh5a367n5vi4uq 14 15 protocols protocol NNS work_sny56posuffadh5a367n5vi4uq 14 16 for for IN work_sny56posuffadh5a367n5vi4uq 14 17 delivery delivery NN work_sny56posuffadh5a367n5vi4uq 14 18 of of IN work_sny56posuffadh5a367n5vi4uq 14 19 genes gene NNS work_sny56posuffadh5a367n5vi4uq 14 20 in in IN work_sny56posuffadh5a367n5vi4uq 14 21 SB SB NNP work_sny56posuffadh5a367n5vi4uq 14 22 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 14 23 to to IN work_sny56posuffadh5a367n5vi4uq 14 24 mammalian mammalian JJ work_sny56posuffadh5a367n5vi4uq 14 25 chromosomes chromosome NNS work_sny56posuffadh5a367n5vi4uq 14 26 in in IN work_sny56posuffadh5a367n5vi4uq 14 27 living live VBG work_sny56posuffadh5a367n5vi4uq 14 28 animals animal NNS work_sny56posuffadh5a367n5vi4uq 14 29 . . . work_sny56posuffadh5a367n5vi4uq 15 1 Copyright copyright NN work_sny56posuffadh5a367n5vi4uq 15 2   CD work_sny56posuffadh5a367n5vi4uq 15 3 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 15 4 John John NNP work_sny56posuffadh5a367n5vi4uq 15 5 Wiley Wiley NNP work_sny56posuffadh5a367n5vi4uq 15 6 & & CC work_sny56posuffadh5a367n5vi4uq 15 7 Sons Sons NNPS work_sny56posuffadh5a367n5vi4uq 15 8 , , , work_sny56posuffadh5a367n5vi4uq 15 9 Ltd. Ltd. NNP work_sny56posuffadh5a367n5vi4uq 15 10 Keywords Keywords NNP work_sny56posuffadh5a367n5vi4uq 15 11 footprint footprint NN work_sny56posuffadh5a367n5vi4uq 15 12 ; ; : work_sny56posuffadh5a367n5vi4uq 15 13 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 15 14 liver liver NN work_sny56posuffadh5a367n5vi4uq 15 15 ; ; : work_sny56posuffadh5a367n5vi4uq 15 16 Tc1 Tc1 NNP work_sny56posuffadh5a367n5vi4uq 15 17 / / SYM work_sny56posuffadh5a367n5vi4uq 15 18 mariner mariner NN work_sny56posuffadh5a367n5vi4uq 15 19 ; ; , work_sny56posuffadh5a367n5vi4uq 15 20 terminal terminal JJ work_sny56posuffadh5a367n5vi4uq 15 21 repeats repeat NNS work_sny56posuffadh5a367n5vi4uq 15 22 ; ; : work_sny56posuffadh5a367n5vi4uq 15 23 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 15 24 Introduction introduction NN work_sny56posuffadh5a367n5vi4uq 15 25 Tc1 Tc1 NNP work_sny56posuffadh5a367n5vi4uq 15 26 / / SYM work_sny56posuffadh5a367n5vi4uq 15 27 mariner mariner NN work_sny56posuffadh5a367n5vi4uq 15 28 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 15 29 are be VBP work_sny56posuffadh5a367n5vi4uq 15 30 a a DT work_sny56posuffadh5a367n5vi4uq 15 31 large large JJ work_sny56posuffadh5a367n5vi4uq 15 32 family family NN work_sny56posuffadh5a367n5vi4uq 15 33 of of IN work_sny56posuffadh5a367n5vi4uq 15 34 DNA dna NN work_sny56posuffadh5a367n5vi4uq 15 35 transposable transposable JJ work_sny56posuffadh5a367n5vi4uq 15 36 elements element NNS work_sny56posuffadh5a367n5vi4uq 15 37 . . . work_sny56posuffadh5a367n5vi4uq 16 1 They -PRON- PRP work_sny56posuffadh5a367n5vi4uq 16 2 are be VBP work_sny56posuffadh5a367n5vi4uq 16 3 widespread widespread JJ work_sny56posuffadh5a367n5vi4uq 16 4 in in IN work_sny56posuffadh5a367n5vi4uq 16 5 nature nature NN work_sny56posuffadh5a367n5vi4uq 16 6 and and CC work_sny56posuffadh5a367n5vi4uq 16 7 can can MD work_sny56posuffadh5a367n5vi4uq 16 8 be be VB work_sny56posuffadh5a367n5vi4uq 16 9 found find VBN work_sny56posuffadh5a367n5vi4uq 16 10 in in IN work_sny56posuffadh5a367n5vi4uq 16 11 virtually virtually RB work_sny56posuffadh5a367n5vi4uq 16 12 all all DT work_sny56posuffadh5a367n5vi4uq 16 13 animal animal NN work_sny56posuffadh5a367n5vi4uq 16 14 phyla phyla NN work_sny56posuffadh5a367n5vi4uq 16 15 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 16 16 1,2 1,2 CD work_sny56posuffadh5a367n5vi4uq 16 17 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 16 18 . . . work_sny56posuffadh5a367n5vi4uq 17 1 Recently recently RB work_sny56posuffadh5a367n5vi4uq 17 2 , , , work_sny56posuffadh5a367n5vi4uq 17 3 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 17 4 reconstructed reconstruct VBD work_sny56posuffadh5a367n5vi4uq 17 5 a a DT work_sny56posuffadh5a367n5vi4uq 17 6 synthetic synthetic JJ work_sny56posuffadh5a367n5vi4uq 17 7 Tc1-like tc1-like JJ work_sny56posuffadh5a367n5vi4uq 17 8 vertebrate vertebrate NN work_sny56posuffadh5a367n5vi4uq 17 9 transposon transposon NN work_sny56posuffadh5a367n5vi4uq 17 10 system system NN work_sny56posuffadh5a367n5vi4uq 17 11 called call VBD work_sny56posuffadh5a367n5vi4uq 17 12 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 17 13 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 17 14 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 17 15 SB SB NNP work_sny56posuffadh5a367n5vi4uq 17 16 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 17 17 from from IN work_sny56posuffadh5a367n5vi4uq 17 18 inactive inactive JJ work_sny56posuffadh5a367n5vi4uq 17 19 salmonid salmonid JJ work_sny56posuffadh5a367n5vi4uq 17 20 fish fish NN work_sny56posuffadh5a367n5vi4uq 17 21 elements element NNS work_sny56posuffadh5a367n5vi4uq 17 22 by by IN work_sny56posuffadh5a367n5vi4uq 17 23 a a DT work_sny56posuffadh5a367n5vi4uq 17 24 comparative comparative JJ work_sny56posuffadh5a367n5vi4uq 17 25 phylogenetic phylogenetic JJ work_sny56posuffadh5a367n5vi4uq 17 26 approach approach NN work_sny56posuffadh5a367n5vi4uq 17 27 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 17 28 3 3 CD work_sny56posuffadh5a367n5vi4uq 17 29 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 17 30 . . . work_sny56posuffadh5a367n5vi4uq 18 1 In in IN work_sny56posuffadh5a367n5vi4uq 18 2 mammalian mammalian JJ work_sny56posuffadh5a367n5vi4uq 18 3 cells cell NNS work_sny56posuffadh5a367n5vi4uq 18 4 , , , work_sny56posuffadh5a367n5vi4uq 18 5 the the DT work_sny56posuffadh5a367n5vi4uq 18 6 SB SB NNP work_sny56posuffadh5a367n5vi4uq 18 7 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 18 8 system system NN work_sny56posuffadh5a367n5vi4uq 18 9 is be VBZ work_sny56posuffadh5a367n5vi4uq 18 10 an an DT work_sny56posuffadh5a367n5vi4uq 18 11 order order NN work_sny56posuffadh5a367n5vi4uq 18 12 of of IN work_sny56posuffadh5a367n5vi4uq 18 13 magnitude magnitude NN work_sny56posuffadh5a367n5vi4uq 18 14 more more RBR work_sny56posuffadh5a367n5vi4uq 18 15 efficient efficient JJ work_sny56posuffadh5a367n5vi4uq 18 16 than than IN work_sny56posuffadh5a367n5vi4uq 18 17 other other JJ work_sny56posuffadh5a367n5vi4uq 18 18 Tc1 tc1 NN work_sny56posuffadh5a367n5vi4uq 18 19 family family NN work_sny56posuffadh5a367n5vi4uq 18 20 members member NNS work_sny56posuffadh5a367n5vi4uq 18 21 tested test VBN work_sny56posuffadh5a367n5vi4uq 18 22 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 18 23 4 4 CD work_sny56posuffadh5a367n5vi4uq 18 24 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 18 25 . . . work_sny56posuffadh5a367n5vi4uq 19 1 Since since IN work_sny56posuffadh5a367n5vi4uq 19 2 its -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 19 3 discovery discovery NN work_sny56posuffadh5a367n5vi4uq 19 4 , , , work_sny56posuffadh5a367n5vi4uq 19 5 SB SB NNP work_sny56posuffadh5a367n5vi4uq 19 6 has have VBZ work_sny56posuffadh5a367n5vi4uq 19 7 been be VBN work_sny56posuffadh5a367n5vi4uq 19 8 used use VBN work_sny56posuffadh5a367n5vi4uq 19 9 in in IN work_sny56posuffadh5a367n5vi4uq 19 10 a a DT work_sny56posuffadh5a367n5vi4uq 19 11 wide wide JJ work_sny56posuffadh5a367n5vi4uq 19 12 range range NN work_sny56posuffadh5a367n5vi4uq 19 13 of of IN work_sny56posuffadh5a367n5vi4uq 19 14 vertebrate vertebrate NN work_sny56posuffadh5a367n5vi4uq 19 15 systems system NNS work_sny56posuffadh5a367n5vi4uq 19 16 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 19 17 5 5 CD work_sny56posuffadh5a367n5vi4uq 19 18 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 19 19 for for IN work_sny56posuffadh5a367n5vi4uq 19 20 functional functional JJ work_sny56posuffadh5a367n5vi4uq 19 21 genomics genomic NNS work_sny56posuffadh5a367n5vi4uq 19 22 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 19 23 6 6 CD work_sny56posuffadh5a367n5vi4uq 19 24 – – SYM work_sny56posuffadh5a367n5vi4uq 19 25 8 8 CD work_sny56posuffadh5a367n5vi4uq 19 26 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 19 27 , , , work_sny56posuffadh5a367n5vi4uq 19 28 germ germ NN work_sny56posuffadh5a367n5vi4uq 19 29 - - HYPH work_sny56posuffadh5a367n5vi4uq 19 30 line line NN work_sny56posuffadh5a367n5vi4uq 19 31 transgenesis transgenesis NN work_sny56posuffadh5a367n5vi4uq 19 32 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 19 33 4,6,9 4,6,9 CD work_sny56posuffadh5a367n5vi4uq 19 34 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 19 35 , , , work_sny56posuffadh5a367n5vi4uq 19 36 and and CC work_sny56posuffadh5a367n5vi4uq 19 37 somatic somatic JJ work_sny56posuffadh5a367n5vi4uq 19 38 integration integration NN work_sny56posuffadh5a367n5vi4uq 19 39 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 19 40 10 10 CD work_sny56posuffadh5a367n5vi4uq 19 41 – – : work_sny56posuffadh5a367n5vi4uq 19 42 12 12 CD work_sny56posuffadh5a367n5vi4uq 19 43 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 19 44 . . . work_sny56posuffadh5a367n5vi4uq 20 1 Its -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 20 2 higher high JJR work_sny56posuffadh5a367n5vi4uq 20 3 efficiency efficiency NN work_sny56posuffadh5a367n5vi4uq 20 4 compared compare VBN work_sny56posuffadh5a367n5vi4uq 20 5 to to IN work_sny56posuffadh5a367n5vi4uq 20 6 other other JJ work_sny56posuffadh5a367n5vi4uq 20 7 DNA DNA NNP work_sny56posuffadh5a367n5vi4uq 20 8 - - HYPH work_sny56posuffadh5a367n5vi4uq 20 9 based base VBN work_sny56posuffadh5a367n5vi4uq 20 10 transposable transposable JJ work_sny56posuffadh5a367n5vi4uq 20 11 elements element NNS work_sny56posuffadh5a367n5vi4uq 20 12 , , , work_sny56posuffadh5a367n5vi4uq 20 13 its -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 20 14 ability ability NN work_sny56posuffadh5a367n5vi4uq 20 15 to to TO work_sny56posuffadh5a367n5vi4uq 20 16 accommodate accommodate VB work_sny56posuffadh5a367n5vi4uq 20 17 large large JJ work_sny56posuffadh5a367n5vi4uq 20 18 genetic genetic JJ work_sny56posuffadh5a367n5vi4uq 20 19 cargoes cargo NNS work_sny56posuffadh5a367n5vi4uq 20 20 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 20 21 5,13 5,13 CD work_sny56posuffadh5a367n5vi4uq 20 22 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 20 23 , , , work_sny56posuffadh5a367n5vi4uq 20 24 and and CC work_sny56posuffadh5a367n5vi4uq 20 25 its -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 20 26 presumed presume VBN work_sny56posuffadh5a367n5vi4uq 20 27 safety safety NN work_sny56posuffadh5a367n5vi4uq 20 28 compared compare VBN work_sny56posuffadh5a367n5vi4uq 20 29 to to IN work_sny56posuffadh5a367n5vi4uq 20 30 viral viral JJ work_sny56posuffadh5a367n5vi4uq 20 31 vectors vector NNS work_sny56posuffadh5a367n5vi4uq 20 32 make make VBP work_sny56posuffadh5a367n5vi4uq 20 33 SB SB NNP work_sny56posuffadh5a367n5vi4uq 20 34 a a DT work_sny56posuffadh5a367n5vi4uq 20 35 potentially potentially RB work_sny56posuffadh5a367n5vi4uq 20 36 powerful powerful JJ work_sny56posuffadh5a367n5vi4uq 20 37 tool tool NN work_sny56posuffadh5a367n5vi4uq 20 38 for for IN work_sny56posuffadh5a367n5vi4uq 20 39 human human JJ work_sny56posuffadh5a367n5vi4uq 20 40 gene gene NN work_sny56posuffadh5a367n5vi4uq 20 41 therapy therapy NN work_sny56posuffadh5a367n5vi4uq 20 42 . . . work_sny56posuffadh5a367n5vi4uq 21 1 The the DT work_sny56posuffadh5a367n5vi4uq 21 2 SB SB NNP work_sny56posuffadh5a367n5vi4uq 21 3 transposon transposon NN work_sny56posuffadh5a367n5vi4uq 21 4 system system NN work_sny56posuffadh5a367n5vi4uq 21 5 consists consist VBZ work_sny56posuffadh5a367n5vi4uq 21 6 of of IN work_sny56posuffadh5a367n5vi4uq 21 7 two two CD work_sny56posuffadh5a367n5vi4uq 21 8 parts part NNS work_sny56posuffadh5a367n5vi4uq 21 9 – – : work_sny56posuffadh5a367n5vi4uq 21 10 the the DT work_sny56posuffadh5a367n5vi4uq 21 11 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 21 12 , , , work_sny56posuffadh5a367n5vi4uq 21 13 consisting consist VBG work_sny56posuffadh5a367n5vi4uq 21 14 of of IN work_sny56posuffadh5a367n5vi4uq 21 15 inverted invert VBN work_sny56posuffadh5a367n5vi4uq 21 16 terminal terminal JJ work_sny56posuffadh5a367n5vi4uq 21 17 repeats repeat NNS work_sny56posuffadh5a367n5vi4uq 21 18 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 21 19 ITRs ITRs NNP work_sny56posuffadh5a367n5vi4uq 21 20 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 21 21 , , , work_sny56posuffadh5a367n5vi4uq 21 22 and and CC work_sny56posuffadh5a367n5vi4uq 21 23 the the DT work_sny56posuffadh5a367n5vi4uq 21 24 SB SB NNP work_sny56posuffadh5a367n5vi4uq 21 25 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 21 26 that that WDT work_sny56posuffadh5a367n5vi4uq 21 27 catalyzes catalyze VBZ work_sny56posuffadh5a367n5vi4uq 21 28 the the DT work_sny56posuffadh5a367n5vi4uq 21 29 mobilization mobilization NN work_sny56posuffadh5a367n5vi4uq 21 30 of of IN work_sny56posuffadh5a367n5vi4uq 21 31 the the DT work_sny56posuffadh5a367n5vi4uq 21 32 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 21 33 . . . work_sny56posuffadh5a367n5vi4uq 22 1 Like like IN work_sny56posuffadh5a367n5vi4uq 22 2 other other JJ work_sny56posuffadh5a367n5vi4uq 22 3 members member NNS work_sny56posuffadh5a367n5vi4uq 22 4 of of IN work_sny56posuffadh5a367n5vi4uq 22 5 the the DT work_sny56posuffadh5a367n5vi4uq 22 6 Tc1 tc1 NN work_sny56posuffadh5a367n5vi4uq 22 7 / / SYM work_sny56posuffadh5a367n5vi4uq 22 8 mariner mariner NN work_sny56posuffadh5a367n5vi4uq 22 9 Copyright copyright NN work_sny56posuffadh5a367n5vi4uq 22 10   CD work_sny56posuffadh5a367n5vi4uq 22 11 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 22 12 John John NNP work_sny56posuffadh5a367n5vi4uq 22 13 Wiley Wiley NNP work_sny56posuffadh5a367n5vi4uq 22 14 & & CC work_sny56posuffadh5a367n5vi4uq 22 15 Sons Sons NNPS work_sny56posuffadh5a367n5vi4uq 22 16 , , , work_sny56posuffadh5a367n5vi4uq 22 17 Ltd. Ltd. NNP work_sny56posuffadh5a367n5vi4uq 22 18 Excision excision NN work_sny56posuffadh5a367n5vi4uq 22 19 of of IN work_sny56posuffadh5a367n5vi4uq 22 20 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 22 21 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 22 22 Transposons Transposons NNP work_sny56posuffadh5a367n5vi4uq 22 23 575 575 CD work_sny56posuffadh5a367n5vi4uq 22 24 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 22 25 family family NN work_sny56posuffadh5a367n5vi4uq 22 26 , , , work_sny56posuffadh5a367n5vi4uq 22 27 SB SB NNP work_sny56posuffadh5a367n5vi4uq 22 28 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 22 29 are be VBP work_sny56posuffadh5a367n5vi4uq 22 30 mobilized mobilize VBN work_sny56posuffadh5a367n5vi4uq 22 31 via via IN work_sny56posuffadh5a367n5vi4uq 22 32 a a DT work_sny56posuffadh5a367n5vi4uq 22 33 cut- cut- NN work_sny56posuffadh5a367n5vi4uq 22 34 and and CC work_sny56posuffadh5a367n5vi4uq 22 35 - - HYPH work_sny56posuffadh5a367n5vi4uq 22 36 paste paste NN work_sny56posuffadh5a367n5vi4uq 22 37 mechanism mechanism NN work_sny56posuffadh5a367n5vi4uq 22 38 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 22 39 Figure Figure NNP work_sny56posuffadh5a367n5vi4uq 22 40 1A 1a NN work_sny56posuffadh5a367n5vi4uq 22 41 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 22 42 . . . work_sny56posuffadh5a367n5vi4uq 23 1 There there EX work_sny56posuffadh5a367n5vi4uq 23 2 are be VBP work_sny56posuffadh5a367n5vi4uq 23 3 two two CD work_sny56posuffadh5a367n5vi4uq 23 4 major major JJ work_sny56posuffadh5a367n5vi4uq 23 5 steps step NNS work_sny56posuffadh5a367n5vi4uq 23 6 involved involve VBN work_sny56posuffadh5a367n5vi4uq 23 7 in in IN work_sny56posuffadh5a367n5vi4uq 23 8 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 23 9 , , , work_sny56posuffadh5a367n5vi4uq 23 10 the the DT work_sny56posuffadh5a367n5vi4uq 23 11 excision excision NN work_sny56posuffadh5a367n5vi4uq 23 12 of of IN work_sny56posuffadh5a367n5vi4uq 23 13 the the DT work_sny56posuffadh5a367n5vi4uq 23 14 trans- trans- JJ work_sny56posuffadh5a367n5vi4uq 23 15 poson poson NN work_sny56posuffadh5a367n5vi4uq 23 16 from from IN work_sny56posuffadh5a367n5vi4uq 23 17 the the DT work_sny56posuffadh5a367n5vi4uq 23 18 donor donor NN work_sny56posuffadh5a367n5vi4uq 23 19 site site NN work_sny56posuffadh5a367n5vi4uq 23 20 and and CC work_sny56posuffadh5a367n5vi4uq 23 21 the the DT work_sny56posuffadh5a367n5vi4uq 23 22 integration integration NN work_sny56posuffadh5a367n5vi4uq 23 23 of of IN work_sny56posuffadh5a367n5vi4uq 23 24 the the DT work_sny56posuffadh5a367n5vi4uq 23 25 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 23 26 into into IN work_sny56posuffadh5a367n5vi4uq 23 27 the the DT work_sny56posuffadh5a367n5vi4uq 23 28 target target NN work_sny56posuffadh5a367n5vi4uq 23 29 site site NN work_sny56posuffadh5a367n5vi4uq 23 30 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 23 31 2 2 CD work_sny56posuffadh5a367n5vi4uq 23 32 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 23 33 . . . work_sny56posuffadh5a367n5vi4uq 24 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 24 2 from from IN work_sny56posuffadh5a367n5vi4uq 24 3 the the DT work_sny56posuffadh5a367n5vi4uq 24 4 donor donor NN work_sny56posuffadh5a367n5vi4uq 24 5 site site NN work_sny56posuffadh5a367n5vi4uq 24 6 involves involve VBZ work_sny56posuffadh5a367n5vi4uq 24 7 staggered stagger VBN work_sny56posuffadh5a367n5vi4uq 24 8 , , , work_sny56posuffadh5a367n5vi4uq 24 9 double double JJ work_sny56posuffadh5a367n5vi4uq 24 10 - - HYPH work_sny56posuffadh5a367n5vi4uq 24 11 stranded strand VBN work_sny56posuffadh5a367n5vi4uq 24 12 DNA dna NN work_sny56posuffadh5a367n5vi4uq 24 13 breaks break NNS work_sny56posuffadh5a367n5vi4uq 24 14 at at IN work_sny56posuffadh5a367n5vi4uq 24 15 each each DT work_sny56posuffadh5a367n5vi4uq 24 16 side side NN work_sny56posuffadh5a367n5vi4uq 24 17 of of IN work_sny56posuffadh5a367n5vi4uq 24 18 the the DT work_sny56posuffadh5a367n5vi4uq 24 19 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 24 20 , , , work_sny56posuffadh5a367n5vi4uq 24 21 which which WDT work_sny56posuffadh5a367n5vi4uq 24 22 result result VBP work_sny56posuffadh5a367n5vi4uq 24 23 in in IN work_sny56posuffadh5a367n5vi4uq 24 24 a a DT work_sny56posuffadh5a367n5vi4uq 24 25 small small JJ work_sny56posuffadh5a367n5vi4uq 24 26 num- num- NN work_sny56posuffadh5a367n5vi4uq 24 27 ber ber NN work_sny56posuffadh5a367n5vi4uq 24 28 of of IN work_sny56posuffadh5a367n5vi4uq 24 29 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 24 30 at at IN work_sny56posuffadh5a367n5vi4uq 24 31 the the DT work_sny56posuffadh5a367n5vi4uq 24 32 termini termini NN work_sny56posuffadh5a367n5vi4uq 24 33 of of IN work_sny56posuffadh5a367n5vi4uq 24 34 the the DT work_sny56posuffadh5a367n5vi4uq 24 35 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 24 36 being be VBG work_sny56posuffadh5a367n5vi4uq 24 37 left leave VBN work_sny56posuffadh5a367n5vi4uq 24 38 behind behind RB work_sny56posuffadh5a367n5vi4uq 24 39 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 24 40 2,14 2,14 CD work_sny56posuffadh5a367n5vi4uq 24 41 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 24 42 . . . work_sny56posuffadh5a367n5vi4uq 25 1 The the DT work_sny56posuffadh5a367n5vi4uq 25 2 majority majority NN work_sny56posuffadh5a367n5vi4uq 25 3 of of IN work_sny56posuffadh5a367n5vi4uq 25 4 Tc1 Tc1 NNP work_sny56posuffadh5a367n5vi4uq 25 5 / / SYM work_sny56posuffadh5a367n5vi4uq 25 6 mariner mariner NNP work_sny56posuffadh5a367n5vi4uq 25 7 trans- trans- NN work_sny56posuffadh5a367n5vi4uq 25 8 posons poson NNS work_sny56posuffadh5a367n5vi4uq 25 9 integrate integrate VBP work_sny56posuffadh5a367n5vi4uq 25 10 into into IN work_sny56posuffadh5a367n5vi4uq 25 11 TA TA NNP work_sny56posuffadh5a367n5vi4uq 25 12 - - HYPH work_sny56posuffadh5a367n5vi4uq 25 13 dinucleotide dinucleotide NN work_sny56posuffadh5a367n5vi4uq 25 14 base base NN work_sny56posuffadh5a367n5vi4uq 25 15 pairs pair NNS work_sny56posuffadh5a367n5vi4uq 25 16 in in IN work_sny56posuffadh5a367n5vi4uq 25 17 a a DT work_sny56posuffadh5a367n5vi4uq 25 18 fairly fairly RB work_sny56posuffadh5a367n5vi4uq 25 19 random random JJ work_sny56posuffadh5a367n5vi4uq 25 20 manner manner NN work_sny56posuffadh5a367n5vi4uq 25 21 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 25 22 8,9,15 8,9,15 NNP work_sny56posuffadh5a367n5vi4uq 25 23 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 25 24 . . . work_sny56posuffadh5a367n5vi4uq 26 1 As as IN work_sny56posuffadh5a367n5vi4uq 26 2 a a DT work_sny56posuffadh5a367n5vi4uq 26 3 result result NN work_sny56posuffadh5a367n5vi4uq 26 4 of of IN work_sny56posuffadh5a367n5vi4uq 26 5 the the DT work_sny56posuffadh5a367n5vi4uq 26 6 staggered staggered JJ work_sny56posuffadh5a367n5vi4uq 26 7 cut cut NN work_sny56posuffadh5a367n5vi4uq 26 8 at at IN work_sny56posuffadh5a367n5vi4uq 26 9 the the DT work_sny56posuffadh5a367n5vi4uq 26 10 TA TA NNP work_sny56posuffadh5a367n5vi4uq 26 11 target target NN work_sny56posuffadh5a367n5vi4uq 26 12 sites site NNS work_sny56posuffadh5a367n5vi4uq 26 13 , , , work_sny56posuffadh5a367n5vi4uq 26 14 the the DT work_sny56posuffadh5a367n5vi4uq 26 15 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 26 16 are be VBP work_sny56posuffadh5a367n5vi4uq 26 17 flanked flank VBN work_sny56posuffadh5a367n5vi4uq 26 18 by by IN work_sny56posuffadh5a367n5vi4uq 26 19 TA- TA- NNP work_sny56posuffadh5a367n5vi4uq 26 20 dinucleotides dinucleotide NNS work_sny56posuffadh5a367n5vi4uq 26 21 on on IN work_sny56posuffadh5a367n5vi4uq 26 22 both both DT work_sny56posuffadh5a367n5vi4uq 26 23 sides side NNS work_sny56posuffadh5a367n5vi4uq 26 24 after after IN work_sny56posuffadh5a367n5vi4uq 26 25 integration integration NN work_sny56posuffadh5a367n5vi4uq 26 26 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 26 27 Figure figure NN work_sny56posuffadh5a367n5vi4uq 26 28 1B 1b NN work_sny56posuffadh5a367n5vi4uq 26 29 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 26 30 , , , work_sny56posuffadh5a367n5vi4uq 26 31 a a DT work_sny56posuffadh5a367n5vi4uq 26 32 phenomenon phenomenon NN work_sny56posuffadh5a367n5vi4uq 26 33 called call VBN work_sny56posuffadh5a367n5vi4uq 26 34 target target NN work_sny56posuffadh5a367n5vi4uq 26 35 - - HYPH work_sny56posuffadh5a367n5vi4uq 26 36 site site NN work_sny56posuffadh5a367n5vi4uq 26 37 duplication duplication NN work_sny56posuffadh5a367n5vi4uq 26 38 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 26 39 2,3 2,3 CD work_sny56posuffadh5a367n5vi4uq 26 40 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 26 41 . . . work_sny56posuffadh5a367n5vi4uq 27 1 The the DT work_sny56posuffadh5a367n5vi4uq 27 2 ITRs itr NNS work_sny56posuffadh5a367n5vi4uq 27 3 of of IN work_sny56posuffadh5a367n5vi4uq 27 4 the the DT work_sny56posuffadh5a367n5vi4uq 27 5 current current JJ work_sny56posuffadh5a367n5vi4uq 27 6 SB SB NNP work_sny56posuffadh5a367n5vi4uq 27 7 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 27 8 came come VBD work_sny56posuffadh5a367n5vi4uq 27 9 from from IN work_sny56posuffadh5a367n5vi4uq 27 10 a a DT work_sny56posuffadh5a367n5vi4uq 27 11 single single JJ work_sny56posuffadh5a367n5vi4uq 27 12 Tc1-like tc1-like JJ work_sny56posuffadh5a367n5vi4uq 27 13 element element NN work_sny56posuffadh5a367n5vi4uq 27 14 from from IN work_sny56posuffadh5a367n5vi4uq 27 15 a a DT work_sny56posuffadh5a367n5vi4uq 27 16 salmonid salmonid NN work_sny56posuffadh5a367n5vi4uq 27 17 , , , work_sny56posuffadh5a367n5vi4uq 27 18 Tanichthys Tanichthys NNP work_sny56posuffadh5a367n5vi4uq 27 19 albonuibes albonuibe NNS work_sny56posuffadh5a367n5vi4uq 27 20 – – : work_sny56posuffadh5a367n5vi4uq 27 21 referred refer VBD work_sny56posuffadh5a367n5vi4uq 27 22 to to IN work_sny56posuffadh5a367n5vi4uq 27 23 as as IN work_sny56posuffadh5a367n5vi4uq 27 24 T. T. NNP work_sny56posuffadh5a367n5vi4uq 27 25 T T NNP work_sny56posuffadh5a367n5vi4uq 27 26 has have VBZ work_sny56posuffadh5a367n5vi4uq 27 27 two two CD work_sny56posuffadh5a367n5vi4uq 27 28 inverted invert VBN work_sny56posuffadh5a367n5vi4uq 27 29 repeats repeat NNS work_sny56posuffadh5a367n5vi4uq 27 30 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 27 31 IRs IRs NNP work_sny56posuffadh5a367n5vi4uq 27 32 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 27 33 at at IN work_sny56posuffadh5a367n5vi4uq 27 34 its -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 27 35 termini termini NN work_sny56posuffadh5a367n5vi4uq 27 36 and and CC work_sny56posuffadh5a367n5vi4uq 27 37 two two CD work_sny56posuffadh5a367n5vi4uq 27 38 ‘ ' `` work_sny56posuffadh5a367n5vi4uq 27 39 direct direct JJ work_sny56posuffadh5a367n5vi4uq 27 40 repeats repeat NNS work_sny56posuffadh5a367n5vi4uq 27 41 ’ ' '' work_sny56posuffadh5a367n5vi4uq 27 42 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 27 43 DRs dr NNS work_sny56posuffadh5a367n5vi4uq 27 44 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 27 45 within within IN work_sny56posuffadh5a367n5vi4uq 27 46 each each DT work_sny56posuffadh5a367n5vi4uq 27 47 IR IR NNP work_sny56posuffadh5a367n5vi4uq 27 48 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 27 49 Figure figure NN work_sny56posuffadh5a367n5vi4uq 27 50 1A 1a NN work_sny56posuffadh5a367n5vi4uq 27 51 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 27 52 . . . work_sny56posuffadh5a367n5vi4uq 28 1 The the DT work_sny56posuffadh5a367n5vi4uq 28 2 outer outer JJ work_sny56posuffadh5a367n5vi4uq 28 3 DRs dr NNS work_sny56posuffadh5a367n5vi4uq 28 4 , , , work_sny56posuffadh5a367n5vi4uq 28 5 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 28 6 and and CC work_sny56posuffadh5a367n5vi4uq 28 7 Ro Ro NNP work_sny56posuffadh5a367n5vi4uq 28 8 , , , work_sny56posuffadh5a367n5vi4uq 28 9 are be VBP work_sny56posuffadh5a367n5vi4uq 28 10 located locate VBN work_sny56posuffadh5a367n5vi4uq 28 11 at at IN work_sny56posuffadh5a367n5vi4uq 28 12 the the DT work_sny56posuffadh5a367n5vi4uq 28 13 left left JJ work_sny56posuffadh5a367n5vi4uq 28 14 and and CC work_sny56posuffadh5a367n5vi4uq 28 15 right right JJ work_sny56posuffadh5a367n5vi4uq 28 16 termini termini NN work_sny56posuffadh5a367n5vi4uq 28 17 of of IN work_sny56posuffadh5a367n5vi4uq 28 18 the the DT work_sny56posuffadh5a367n5vi4uq 28 19 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 28 20 , , , work_sny56posuffadh5a367n5vi4uq 28 21 respectively respectively RB work_sny56posuffadh5a367n5vi4uq 28 22 , , , work_sny56posuffadh5a367n5vi4uq 28 23 and and CC work_sny56posuffadh5a367n5vi4uq 28 24 the the DT work_sny56posuffadh5a367n5vi4uq 28 25 inner inner JJ work_sny56posuffadh5a367n5vi4uq 28 26 DRs dr NNS work_sny56posuffadh5a367n5vi4uq 28 27 , , , work_sny56posuffadh5a367n5vi4uq 28 28 Li Li NNP work_sny56posuffadh5a367n5vi4uq 28 29 and and CC work_sny56posuffadh5a367n5vi4uq 28 30 Ri Ri NNP work_sny56posuffadh5a367n5vi4uq 28 31 , , , work_sny56posuffadh5a367n5vi4uq 28 32 are be VBP work_sny56posuffadh5a367n5vi4uq 28 33 located locate VBN work_sny56posuffadh5a367n5vi4uq 28 34 further further RB work_sny56posuffadh5a367n5vi4uq 28 35 inside inside IN work_sny56posuffadh5a367n5vi4uq 28 36 the the DT work_sny56posuffadh5a367n5vi4uq 28 37 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 28 38 . . . work_sny56posuffadh5a367n5vi4uq 29 1 Both both DT work_sny56posuffadh5a367n5vi4uq 29 2 DRs dr NNS work_sny56posuffadh5a367n5vi4uq 29 3 contain contain VBP work_sny56posuffadh5a367n5vi4uq 29 4 binding bind VBG work_sny56posuffadh5a367n5vi4uq 29 5 sites site NNS work_sny56posuffadh5a367n5vi4uq 29 6 for for IN work_sny56posuffadh5a367n5vi4uq 29 7 SB SB NNP work_sny56posuffadh5a367n5vi4uq 29 8 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 29 9 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 29 10 3 3 CD work_sny56posuffadh5a367n5vi4uq 29 11 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 29 12 and and CC work_sny56posuffadh5a367n5vi4uq 29 13 the the DT work_sny56posuffadh5a367n5vi4uq 29 14 middle middle JJ work_sny56posuffadh5a367n5vi4uq 29 15 18 18 CD work_sny56posuffadh5a367n5vi4uq 29 16 bp bp NN work_sny56posuffadh5a367n5vi4uq 29 17 within within IN work_sny56posuffadh5a367n5vi4uq 29 18 the the DT work_sny56posuffadh5a367n5vi4uq 29 19 DRs dr NNS work_sny56posuffadh5a367n5vi4uq 29 20 have have VBP work_sny56posuffadh5a367n5vi4uq 29 21 been be VBN work_sny56posuffadh5a367n5vi4uq 29 22 suggested suggest VBN work_sny56posuffadh5a367n5vi4uq 29 23 to to TO work_sny56posuffadh5a367n5vi4uq 29 24 comprise comprise VB work_sny56posuffadh5a367n5vi4uq 29 25 a a DT work_sny56posuffadh5a367n5vi4uq 29 26 minimal minimal JJ work_sny56posuffadh5a367n5vi4uq 29 27 core core NN work_sny56posuffadh5a367n5vi4uq 29 28 sequence sequence NN work_sny56posuffadh5a367n5vi4uq 29 29 for for IN work_sny56posuffadh5a367n5vi4uq 29 30 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 29 31 binding bind VBG work_sny56posuffadh5a367n5vi4uq 29 32 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 29 33 16 16 CD work_sny56posuffadh5a367n5vi4uq 29 34 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 29 35 . . . work_sny56posuffadh5a367n5vi4uq 30 1 Both both DT work_sny56posuffadh5a367n5vi4uq 30 2 the the DT work_sny56posuffadh5a367n5vi4uq 30 3 outer outer JJ work_sny56posuffadh5a367n5vi4uq 30 4 and and CC work_sny56posuffadh5a367n5vi4uq 30 5 inner inner JJ work_sny56posuffadh5a367n5vi4uq 30 6 DRs dr NNS work_sny56posuffadh5a367n5vi4uq 30 7 are be VBP work_sny56posuffadh5a367n5vi4uq 30 8 required require VBN work_sny56posuffadh5a367n5vi4uq 30 9 for for IN work_sny56posuffadh5a367n5vi4uq 30 10 efficient efficient JJ work_sny56posuffadh5a367n5vi4uq 30 11 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 30 12 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 30 13 3 3 CD work_sny56posuffadh5a367n5vi4uq 30 14 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 30 15 , , , work_sny56posuffadh5a367n5vi4uq 30 16 but but CC work_sny56posuffadh5a367n5vi4uq 30 17 they -PRON- PRP work_sny56posuffadh5a367n5vi4uq 30 18 are be VBP work_sny56posuffadh5a367n5vi4uq 30 19 not not RB work_sny56posuffadh5a367n5vi4uq 30 20 interchangeable interchangeable JJ work_sny56posuffadh5a367n5vi4uq 30 21 , , , work_sny56posuffadh5a367n5vi4uq 30 22 indicating indicate VBG work_sny56posuffadh5a367n5vi4uq 30 23 that that IN work_sny56posuffadh5a367n5vi4uq 30 24 their -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 30 25 roles role NNS work_sny56posuffadh5a367n5vi4uq 30 26 in in IN work_sny56posuffadh5a367n5vi4uq 30 27 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 30 28 are be VBP work_sny56posuffadh5a367n5vi4uq 30 29 different different JJ work_sny56posuffadh5a367n5vi4uq 30 30 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 30 31 16 16 CD work_sny56posuffadh5a367n5vi4uq 30 32 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 30 33 . . . work_sny56posuffadh5a367n5vi4uq 31 1 B B NNP work_sny56posuffadh5a367n5vi4uq 31 2 A a DT work_sny56posuffadh5a367n5vi4uq 31 3 Figure figure NN work_sny56posuffadh5a367n5vi4uq 31 4 1 1 CD work_sny56posuffadh5a367n5vi4uq 31 5 . . . work_sny56posuffadh5a367n5vi4uq 32 1 The the DT work_sny56posuffadh5a367n5vi4uq 32 2 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 32 3 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 32 4 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 32 5 SB SB NNP work_sny56posuffadh5a367n5vi4uq 32 6 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 32 7 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 32 8 and and CC work_sny56posuffadh5a367n5vi4uq 32 9 its -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 32 10 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 32 11 . . . work_sny56posuffadh5a367n5vi4uq 33 1 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 33 2 A a DT work_sny56posuffadh5a367n5vi4uq 33 3 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 33 4 Structure structure NN work_sny56posuffadh5a367n5vi4uq 33 5 of of IN work_sny56posuffadh5a367n5vi4uq 33 6 the the DT work_sny56posuffadh5a367n5vi4uq 33 7 terminal terminal JJ work_sny56posuffadh5a367n5vi4uq 33 8 repeats repeat NNS work_sny56posuffadh5a367n5vi4uq 33 9 of of IN work_sny56posuffadh5a367n5vi4uq 33 10 the the DT work_sny56posuffadh5a367n5vi4uq 33 11 SB SB NNP work_sny56posuffadh5a367n5vi4uq 33 12 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 33 13 . . . work_sny56posuffadh5a367n5vi4uq 34 1 The the DT work_sny56posuffadh5a367n5vi4uq 34 2 DRs dr NNS work_sny56posuffadh5a367n5vi4uq 34 3 of of IN work_sny56posuffadh5a367n5vi4uq 34 4 the the DT work_sny56posuffadh5a367n5vi4uq 34 5 ITRs itr NNS work_sny56posuffadh5a367n5vi4uq 34 6 are be VBP work_sny56posuffadh5a367n5vi4uq 34 7 designated designate VBN work_sny56posuffadh5a367n5vi4uq 34 8 by by IN work_sny56posuffadh5a367n5vi4uq 34 9 arrowheads arrowhead NNS work_sny56posuffadh5a367n5vi4uq 34 10 and and CC work_sny56posuffadh5a367n5vi4uq 34 11 are be VBP work_sny56posuffadh5a367n5vi4uq 34 12 labeled label VBN work_sny56posuffadh5a367n5vi4uq 34 13 according accord VBG work_sny56posuffadh5a367n5vi4uq 34 14 to to IN work_sny56posuffadh5a367n5vi4uq 34 15 their -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 34 16 positions position NNS work_sny56posuffadh5a367n5vi4uq 34 17 in in IN work_sny56posuffadh5a367n5vi4uq 34 18 the the DT work_sny56posuffadh5a367n5vi4uq 34 19 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 34 20 used use VBN work_sny56posuffadh5a367n5vi4uq 34 21 in in IN work_sny56posuffadh5a367n5vi4uq 34 22 this this DT work_sny56posuffadh5a367n5vi4uq 34 23 study study NN work_sny56posuffadh5a367n5vi4uq 34 24 . . . work_sny56posuffadh5a367n5vi4uq 35 1 The the DT work_sny56posuffadh5a367n5vi4uq 35 2 boxed boxed JJ work_sny56posuffadh5a367n5vi4uq 35 3 TAs ta NNS work_sny56posuffadh5a367n5vi4uq 35 4 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 35 5 the the DT work_sny56posuffadh5a367n5vi4uq 35 6 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 35 7 result result VBP work_sny56posuffadh5a367n5vi4uq 35 8 from from IN work_sny56posuffadh5a367n5vi4uq 35 9 duplication duplication NN work_sny56posuffadh5a367n5vi4uq 35 10 of of IN work_sny56posuffadh5a367n5vi4uq 35 11 the the DT work_sny56posuffadh5a367n5vi4uq 35 12 original original JJ work_sny56posuffadh5a367n5vi4uq 35 13 TA TA NNP work_sny56posuffadh5a367n5vi4uq 35 14 insertion insertion NN work_sny56posuffadh5a367n5vi4uq 35 15 site site NN work_sny56posuffadh5a367n5vi4uq 35 16 . . . work_sny56posuffadh5a367n5vi4uq 36 1 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 36 2 B b NN work_sny56posuffadh5a367n5vi4uq 36 3 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 36 4 ‘ ' `` work_sny56posuffadh5a367n5vi4uq 36 5 ‘ ' `` work_sny56posuffadh5a367n5vi4uq 36 6 Cut Cut NNP work_sny56posuffadh5a367n5vi4uq 36 7 - - HYPH work_sny56posuffadh5a367n5vi4uq 36 8 and and CC work_sny56posuffadh5a367n5vi4uq 36 9 - - HYPH work_sny56posuffadh5a367n5vi4uq 36 10 paste paste NN work_sny56posuffadh5a367n5vi4uq 36 11 ’ ' '' work_sny56posuffadh5a367n5vi4uq 36 12 ’ ' '' work_sny56posuffadh5a367n5vi4uq 36 13 mechanism mechanism NN work_sny56posuffadh5a367n5vi4uq 36 14 of of IN work_sny56posuffadh5a367n5vi4uq 36 15 SB SB NNP work_sny56posuffadh5a367n5vi4uq 36 16 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 36 17 revised revise VBN work_sny56posuffadh5a367n5vi4uq 36 18 from from IN work_sny56posuffadh5a367n5vi4uq 36 19 Luo Luo NNP work_sny56posuffadh5a367n5vi4uq 36 20 et et NNP work_sny56posuffadh5a367n5vi4uq 36 21 al al NNP work_sny56posuffadh5a367n5vi4uq 36 22 . . . work_sny56posuffadh5a367n5vi4uq 37 1 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 37 2 14 14 CD work_sny56posuffadh5a367n5vi4uq 37 3 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 37 4 and and CC work_sny56posuffadh5a367n5vi4uq 37 5 Plasterk Plasterk NNP work_sny56posuffadh5a367n5vi4uq 37 6 et et NNP work_sny56posuffadh5a367n5vi4uq 37 7 al al NNP work_sny56posuffadh5a367n5vi4uq 37 8 . . . work_sny56posuffadh5a367n5vi4uq 38 1 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 38 2 2 2 CD work_sny56posuffadh5a367n5vi4uq 38 3 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 38 4 . . . work_sny56posuffadh5a367n5vi4uq 39 1 The the DT work_sny56posuffadh5a367n5vi4uq 39 2 two two CD work_sny56posuffadh5a367n5vi4uq 39 3 major major JJ work_sny56posuffadh5a367n5vi4uq 39 4 steps step NNS work_sny56posuffadh5a367n5vi4uq 39 5 involved involve VBN work_sny56posuffadh5a367n5vi4uq 39 6 in in IN work_sny56posuffadh5a367n5vi4uq 39 7 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 39 8 , , , work_sny56posuffadh5a367n5vi4uq 39 9 excision excision NN work_sny56posuffadh5a367n5vi4uq 39 10 and and CC work_sny56posuffadh5a367n5vi4uq 39 11 integration integration NN work_sny56posuffadh5a367n5vi4uq 39 12 of of IN work_sny56posuffadh5a367n5vi4uq 39 13 a a DT work_sny56posuffadh5a367n5vi4uq 39 14 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 39 15 are be VBP work_sny56posuffadh5a367n5vi4uq 39 16 shown show VBN work_sny56posuffadh5a367n5vi4uq 39 17 . . . work_sny56posuffadh5a367n5vi4uq 40 1 The the DT work_sny56posuffadh5a367n5vi4uq 40 2 two two CD work_sny56posuffadh5a367n5vi4uq 40 3 broken broken JJ work_sny56posuffadh5a367n5vi4uq 40 4 ends end NNS work_sny56posuffadh5a367n5vi4uq 40 5 at at IN work_sny56posuffadh5a367n5vi4uq 40 6 the the DT work_sny56posuffadh5a367n5vi4uq 40 7 donor donor NN work_sny56posuffadh5a367n5vi4uq 40 8 sites site NNS work_sny56posuffadh5a367n5vi4uq 40 9 , , , work_sny56posuffadh5a367n5vi4uq 40 10 joined join VBN work_sny56posuffadh5a367n5vi4uq 40 11 together together RB work_sny56posuffadh5a367n5vi4uq 40 12 by by IN work_sny56posuffadh5a367n5vi4uq 40 13 non non JJ work_sny56posuffadh5a367n5vi4uq 40 14 - - JJ work_sny56posuffadh5a367n5vi4uq 40 15 homologous homologous JJ work_sny56posuffadh5a367n5vi4uq 40 16 end end NN work_sny56posuffadh5a367n5vi4uq 40 17 - - HYPH work_sny56posuffadh5a367n5vi4uq 40 18 joining joining NN work_sny56posuffadh5a367n5vi4uq 40 19 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 40 20 NHEJ NHEJ NNP work_sny56posuffadh5a367n5vi4uq 40 21 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 40 22 enzymes enzyme NNS work_sny56posuffadh5a367n5vi4uq 40 23 encoded encode VBN work_sny56posuffadh5a367n5vi4uq 40 24 by by IN work_sny56posuffadh5a367n5vi4uq 40 25 the the DT work_sny56posuffadh5a367n5vi4uq 40 26 host host NN work_sny56posuffadh5a367n5vi4uq 40 27 , , , work_sny56posuffadh5a367n5vi4uq 40 28 leave leave VB work_sny56posuffadh5a367n5vi4uq 40 29 a a DT work_sny56posuffadh5a367n5vi4uq 40 30 footprint footprint NN work_sny56posuffadh5a367n5vi4uq 40 31 at at IN work_sny56posuffadh5a367n5vi4uq 40 32 the the DT work_sny56posuffadh5a367n5vi4uq 40 33 donor donor NN work_sny56posuffadh5a367n5vi4uq 40 34 site site NN work_sny56posuffadh5a367n5vi4uq 40 35 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 40 36 - - HYPH work_sny56posuffadh5a367n5vi4uq 40 37 based base VBN work_sny56posuffadh5a367n5vi4uq 40 38 excision excision NN work_sny56posuffadh5a367n5vi4uq 40 39 analysis analysis NN work_sny56posuffadh5a367n5vi4uq 40 40 has have VBZ work_sny56posuffadh5a367n5vi4uq 40 41 been be VBN work_sny56posuffadh5a367n5vi4uq 40 42 used use VBN work_sny56posuffadh5a367n5vi4uq 40 43 to to TO work_sny56posuffadh5a367n5vi4uq 40 44 detect detect VB work_sny56posuffadh5a367n5vi4uq 40 45 excision excision NN work_sny56posuffadh5a367n5vi4uq 40 46 from from IN work_sny56posuffadh5a367n5vi4uq 40 47 a a DT work_sny56posuffadh5a367n5vi4uq 40 48 chromosomal chromosomal JJ work_sny56posuffadh5a367n5vi4uq 40 49 location location NN work_sny56posuffadh5a367n5vi4uq 40 50 by by IN work_sny56posuffadh5a367n5vi4uq 40 51 SB SB NNP work_sny56posuffadh5a367n5vi4uq 40 52 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 40 53 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 40 54 4,7,9,14 4,7,9,14 NN work_sny56posuffadh5a367n5vi4uq 40 55 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 40 56 . . . work_sny56posuffadh5a367n5vi4uq 41 1 However however RB work_sny56posuffadh5a367n5vi4uq 41 2 , , , work_sny56posuffadh5a367n5vi4uq 41 3 this this DT work_sny56posuffadh5a367n5vi4uq 41 4 method method NN work_sny56posuffadh5a367n5vi4uq 41 5 is be VBZ work_sny56posuffadh5a367n5vi4uq 41 6 not not RB work_sny56posuffadh5a367n5vi4uq 41 7 suitable suitable JJ work_sny56posuffadh5a367n5vi4uq 41 8 for for IN work_sny56posuffadh5a367n5vi4uq 41 9 studying study VBG work_sny56posuffadh5a367n5vi4uq 41 10 the the DT work_sny56posuffadh5a367n5vi4uq 41 11 mechanism mechanism NN work_sny56posuffadh5a367n5vi4uq 41 12 of of IN work_sny56posuffadh5a367n5vi4uq 41 13 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 41 14 because because IN work_sny56posuffadh5a367n5vi4uq 41 15 the the DT work_sny56posuffadh5a367n5vi4uq 41 16 excision excision NN work_sny56posuffadh5a367n5vi4uq 41 17 is be VBZ work_sny56posuffadh5a367n5vi4uq 41 18 limited limit VBN work_sny56posuffadh5a367n5vi4uq 41 19 to to IN work_sny56posuffadh5a367n5vi4uq 41 20 a a DT work_sny56posuffadh5a367n5vi4uq 41 21 particular particular JJ work_sny56posuffadh5a367n5vi4uq 41 22 transposon transposon NN work_sny56posuffadh5a367n5vi4uq 41 23 and and CC work_sny56posuffadh5a367n5vi4uq 41 24 a a DT work_sny56posuffadh5a367n5vi4uq 41 25 particular particular JJ work_sny56posuffadh5a367n5vi4uq 41 26 donor donor NN work_sny56posuffadh5a367n5vi4uq 41 27 site site NN work_sny56posuffadh5a367n5vi4uq 41 28 in in IN work_sny56posuffadh5a367n5vi4uq 41 29 the the DT work_sny56posuffadh5a367n5vi4uq 41 30 chromosomal chromosomal JJ work_sny56posuffadh5a367n5vi4uq 41 31 position position NN work_sny56posuffadh5a367n5vi4uq 41 32 . . . work_sny56posuffadh5a367n5vi4uq 42 1 Plasmid Plasmid NNP work_sny56posuffadh5a367n5vi4uq 42 2 - - HYPH work_sny56posuffadh5a367n5vi4uq 42 3 based base VBN work_sny56posuffadh5a367n5vi4uq 42 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 42 5 assays assays RB work_sny56posuffadh5a367n5vi4uq 42 6 , , , work_sny56posuffadh5a367n5vi4uq 42 7 on on IN work_sny56posuffadh5a367n5vi4uq 42 8 the the DT work_sny56posuffadh5a367n5vi4uq 42 9 other other JJ work_sny56posuffadh5a367n5vi4uq 42 10 hand hand NN work_sny56posuffadh5a367n5vi4uq 42 11 , , , work_sny56posuffadh5a367n5vi4uq 42 12 are be VBP work_sny56posuffadh5a367n5vi4uq 42 13 more more RBR work_sny56posuffadh5a367n5vi4uq 42 14 versatile versatile JJ work_sny56posuffadh5a367n5vi4uq 42 15 and and CC work_sny56posuffadh5a367n5vi4uq 42 16 easier easy JJR work_sny56posuffadh5a367n5vi4uq 42 17 to to TO work_sny56posuffadh5a367n5vi4uq 42 18 perform perform VB work_sny56posuffadh5a367n5vi4uq 42 19 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 42 20 18,19 18,19 CD work_sny56posuffadh5a367n5vi4uq 42 21 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 42 22 . . . work_sny56posuffadh5a367n5vi4uq 43 1 Hence hence RB work_sny56posuffadh5a367n5vi4uq 43 2 , , , work_sny56posuffadh5a367n5vi4uq 43 3 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 43 4 developed develop VBD work_sny56posuffadh5a367n5vi4uq 43 5 a a DT work_sny56posuffadh5a367n5vi4uq 43 6 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 43 7 - - HYPH work_sny56posuffadh5a367n5vi4uq 43 8 based base VBN work_sny56posuffadh5a367n5vi4uq 43 9 excision excision NN work_sny56posuffadh5a367n5vi4uq 43 10 assay assay NN work_sny56posuffadh5a367n5vi4uq 43 11 for for IN work_sny56posuffadh5a367n5vi4uq 43 12 the the DT work_sny56posuffadh5a367n5vi4uq 43 13 SB SB NNP work_sny56posuffadh5a367n5vi4uq 43 14 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 43 15 system system NN work_sny56posuffadh5a367n5vi4uq 43 16 to to TO work_sny56posuffadh5a367n5vi4uq 43 17 study study VB work_sny56posuffadh5a367n5vi4uq 43 18 the the DT work_sny56posuffadh5a367n5vi4uq 43 19 different different JJ work_sny56posuffadh5a367n5vi4uq 43 20 steps step NNS work_sny56posuffadh5a367n5vi4uq 43 21 of of IN work_sny56posuffadh5a367n5vi4uq 43 22 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 43 23 in in IN work_sny56posuffadh5a367n5vi4uq 43 24 more more JJR work_sny56posuffadh5a367n5vi4uq 43 25 detail detail NN work_sny56posuffadh5a367n5vi4uq 43 26 and and CC work_sny56posuffadh5a367n5vi4uq 43 27 found find VBD work_sny56posuffadh5a367n5vi4uq 43 28 a a DT work_sny56posuffadh5a367n5vi4uq 43 29 correlation correlation NN work_sny56posuffadh5a367n5vi4uq 43 30 between between IN work_sny56posuffadh5a367n5vi4uq 43 31 the the DT work_sny56posuffadh5a367n5vi4uq 43 32 excision excision NN work_sny56posuffadh5a367n5vi4uq 43 33 rate rate NN work_sny56posuffadh5a367n5vi4uq 43 34 and and CC work_sny56posuffadh5a367n5vi4uq 43 35 the the DT work_sny56posuffadh5a367n5vi4uq 43 36 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 43 37 rate rate NN work_sny56posuffadh5a367n5vi4uq 43 38 . . . work_sny56posuffadh5a367n5vi4uq 44 1 Using use VBG work_sny56posuffadh5a367n5vi4uq 44 2 this this DT work_sny56posuffadh5a367n5vi4uq 44 3 assay assay NN work_sny56posuffadh5a367n5vi4uq 44 4 , , , work_sny56posuffadh5a367n5vi4uq 44 5 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 44 6 analyzed analyze VBD work_sny56posuffadh5a367n5vi4uq 44 7 the the DT work_sny56posuffadh5a367n5vi4uq 44 8 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 44 9 of of IN work_sny56posuffadh5a367n5vi4uq 44 10 SB SB NNP work_sny56posuffadh5a367n5vi4uq 44 11 in in IN work_sny56posuffadh5a367n5vi4uq 44 12 tissue tissue NN work_sny56posuffadh5a367n5vi4uq 44 13 - - HYPH work_sny56posuffadh5a367n5vi4uq 44 14 cultured cultured JJ work_sny56posuffadh5a367n5vi4uq 44 15 cells cell NNS work_sny56posuffadh5a367n5vi4uq 44 16 as as RB work_sny56posuffadh5a367n5vi4uq 44 17 well well RB work_sny56posuffadh5a367n5vi4uq 44 18 as as IN work_sny56posuffadh5a367n5vi4uq 44 19 in in IN work_sny56posuffadh5a367n5vi4uq 44 20 zebrafish zebrafish NNP work_sny56posuffadh5a367n5vi4uq 44 21 embryos embryo NNS work_sny56posuffadh5a367n5vi4uq 44 22 and and CC work_sny56posuffadh5a367n5vi4uq 44 23 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 44 24 . . . work_sny56posuffadh5a367n5vi4uq 45 1 The the DT work_sny56posuffadh5a367n5vi4uq 45 2 results result NNS work_sny56posuffadh5a367n5vi4uq 45 3 of of IN work_sny56posuffadh5a367n5vi4uq 45 4 these these DT work_sny56posuffadh5a367n5vi4uq 45 5 studies study NNS work_sny56posuffadh5a367n5vi4uq 45 6 directed direct VBD work_sny56posuffadh5a367n5vi4uq 45 7 design design NN work_sny56posuffadh5a367n5vi4uq 45 8 of of IN work_sny56posuffadh5a367n5vi4uq 45 9 a a DT work_sny56posuffadh5a367n5vi4uq 45 10 better well JJR work_sny56posuffadh5a367n5vi4uq 45 11 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 45 12 as as RB work_sny56posuffadh5a367n5vi4uq 45 13 well well RB work_sny56posuffadh5a367n5vi4uq 45 14 as as IN work_sny56posuffadh5a367n5vi4uq 45 15 led lead VBD work_sny56posuffadh5a367n5vi4uq 45 16 to to IN work_sny56posuffadh5a367n5vi4uq 45 17 the the DT work_sny56posuffadh5a367n5vi4uq 45 18 development development NN work_sny56posuffadh5a367n5vi4uq 45 19 of of IN work_sny56posuffadh5a367n5vi4uq 45 20 a a DT work_sny56posuffadh5a367n5vi4uq 45 21 method method NN work_sny56posuffadh5a367n5vi4uq 45 22 for for IN work_sny56posuffadh5a367n5vi4uq 45 23 determining determine VBG work_sny56posuffadh5a367n5vi4uq 45 24 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 45 25 from from IN work_sny56posuffadh5a367n5vi4uq 45 26 a a DT work_sny56posuffadh5a367n5vi4uq 45 27 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 45 28 in in IN work_sny56posuffadh5a367n5vi4uq 45 29 organs organ NNS work_sny56posuffadh5a367n5vi4uq 45 30 of of IN work_sny56posuffadh5a367n5vi4uq 45 31 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 45 32 . . . work_sny56posuffadh5a367n5vi4uq 46 1 This this DT work_sny56posuffadh5a367n5vi4uq 46 2 latter latter JJ work_sny56posuffadh5a367n5vi4uq 46 3 assay assay NN work_sny56posuffadh5a367n5vi4uq 46 4 should should MD work_sny56posuffadh5a367n5vi4uq 46 5 be be VB work_sny56posuffadh5a367n5vi4uq 46 6 extremely extremely RB work_sny56posuffadh5a367n5vi4uq 46 7 useful useful JJ work_sny56posuffadh5a367n5vi4uq 46 8 for for IN work_sny56posuffadh5a367n5vi4uq 46 9 optimizing optimize VBG work_sny56posuffadh5a367n5vi4uq 46 10 SB SB NNP work_sny56posuffadh5a367n5vi4uq 46 11 - - HYPH work_sny56posuffadh5a367n5vi4uq 46 12 mediated mediate VBN work_sny56posuffadh5a367n5vi4uq 46 13 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 46 14 reactions reaction NNS work_sny56posuffadh5a367n5vi4uq 46 15 in in IN work_sny56posuffadh5a367n5vi4uq 46 16 mammals mammal NNS work_sny56posuffadh5a367n5vi4uq 46 17 as as IN work_sny56posuffadh5a367n5vi4uq 46 18 a a DT work_sny56posuffadh5a367n5vi4uq 46 19 step step NN work_sny56posuffadh5a367n5vi4uq 46 20 towards towards IN work_sny56posuffadh5a367n5vi4uq 46 21 harnessing harness VBG work_sny56posuffadh5a367n5vi4uq 46 22 the the DT work_sny56posuffadh5a367n5vi4uq 46 23 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 46 24 for for IN work_sny56posuffadh5a367n5vi4uq 46 25 gene gene NN work_sny56posuffadh5a367n5vi4uq 46 26 therapy therapy NN work_sny56posuffadh5a367n5vi4uq 46 27 . . . work_sny56posuffadh5a367n5vi4uq 47 1 In in IN work_sny56posuffadh5a367n5vi4uq 47 2 such such JJ work_sny56posuffadh5a367n5vi4uq 47 3 procedures procedure NNS work_sny56posuffadh5a367n5vi4uq 47 4 , , , work_sny56posuffadh5a367n5vi4uq 47 5 a a DT work_sny56posuffadh5a367n5vi4uq 47 6 gene gene NN work_sny56posuffadh5a367n5vi4uq 47 7 contained contain VBN work_sny56posuffadh5a367n5vi4uq 47 8 in in IN work_sny56posuffadh5a367n5vi4uq 47 9 a a DT work_sny56posuffadh5a367n5vi4uq 47 10 transposon transposon JJ work_sny56posuffadh5a367n5vi4uq 47 11 vector vector NN work_sny56posuffadh5a367n5vi4uq 47 12 is be VBZ work_sny56posuffadh5a367n5vi4uq 47 13 prepared prepare VBN work_sny56posuffadh5a367n5vi4uq 47 14 at at IN work_sny56posuffadh5a367n5vi4uq 47 15 high high JJ work_sny56posuffadh5a367n5vi4uq 47 16 concentrations concentration NNS work_sny56posuffadh5a367n5vi4uq 47 17 in in IN work_sny56posuffadh5a367n5vi4uq 47 18 a a DT work_sny56posuffadh5a367n5vi4uq 47 19 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 47 20 that that WDT work_sny56posuffadh5a367n5vi4uq 47 21 is be VBZ work_sny56posuffadh5a367n5vi4uq 47 22 then then RB work_sny56posuffadh5a367n5vi4uq 47 23 delivered deliver VBN work_sny56posuffadh5a367n5vi4uq 47 24 by by IN work_sny56posuffadh5a367n5vi4uq 47 25 any any DT work_sny56posuffadh5a367n5vi4uq 47 26 of of IN work_sny56posuffadh5a367n5vi4uq 47 27 several several JJ work_sny56posuffadh5a367n5vi4uq 47 28 methods method NNS work_sny56posuffadh5a367n5vi4uq 47 29 to to IN work_sny56posuffadh5a367n5vi4uq 47 30 a a DT work_sny56posuffadh5a367n5vi4uq 47 31 target target NN work_sny56posuffadh5a367n5vi4uq 47 32 organ organ NN work_sny56posuffadh5a367n5vi4uq 47 33 . . . work_sny56posuffadh5a367n5vi4uq 48 1 As as IN work_sny56posuffadh5a367n5vi4uq 48 2 a a DT work_sny56posuffadh5a367n5vi4uq 48 3 result result NN work_sny56posuffadh5a367n5vi4uq 48 4 , , , work_sny56posuffadh5a367n5vi4uq 48 5 the the DT work_sny56posuffadh5a367n5vi4uq 48 6 sequence sequence NN work_sny56posuffadh5a367n5vi4uq 48 7 of of IN work_sny56posuffadh5a367n5vi4uq 48 8 the the DT work_sny56posuffadh5a367n5vi4uq 48 9 donor donor NN work_sny56posuffadh5a367n5vi4uq 48 10 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 48 11 can can MD work_sny56posuffadh5a367n5vi4uq 48 12 serve serve VB work_sny56posuffadh5a367n5vi4uq 48 13 as as IN work_sny56posuffadh5a367n5vi4uq 48 14 a a DT work_sny56posuffadh5a367n5vi4uq 48 15 common common JJ work_sny56posuffadh5a367n5vi4uq 48 16 site site NN work_sny56posuffadh5a367n5vi4uq 48 17 for for IN work_sny56posuffadh5a367n5vi4uq 48 18 monitoring monitor VBG work_sny56posuffadh5a367n5vi4uq 48 19 the the DT work_sny56posuffadh5a367n5vi4uq 48 20 excision excision NN work_sny56posuffadh5a367n5vi4uq 48 21 step step NN work_sny56posuffadh5a367n5vi4uq 48 22 of of IN work_sny56posuffadh5a367n5vi4uq 48 23 the the DT work_sny56posuffadh5a367n5vi4uq 48 24 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 48 25 reaction reaction NN work_sny56posuffadh5a367n5vi4uq 48 26 . . . work_sny56posuffadh5a367n5vi4uq 49 1 Materials material NNS work_sny56posuffadh5a367n5vi4uq 49 2 and and CC work_sny56posuffadh5a367n5vi4uq 49 3 methods method NNS work_sny56posuffadh5a367n5vi4uq 49 4 Plasmids plasmid NNS work_sny56posuffadh5a367n5vi4uq 49 5 The the DT work_sny56posuffadh5a367n5vi4uq 49 6 maps map NNS work_sny56posuffadh5a367n5vi4uq 49 7 and and CC work_sny56posuffadh5a367n5vi4uq 49 8 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 49 9 for for IN work_sny56posuffadh5a367n5vi4uq 49 10 plasmids plasmid NNS work_sny56posuffadh5a367n5vi4uq 49 11 pCMVSB pcmvsb NN work_sny56posuffadh5a367n5vi4uq 49 12 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 49 13 also also RB work_sny56posuffadh5a367n5vi4uq 49 14 called call VBN work_sny56posuffadh5a367n5vi4uq 49 15 pSB10 pSB10 NNP work_sny56posuffadh5a367n5vi4uq 49 16 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 49 17 and and CC work_sny56posuffadh5a367n5vi4uq 49 18 pSB10- pSB10- NNP work_sny56posuffadh5a367n5vi4uq 49 19 � � NNP work_sny56posuffadh5a367n5vi4uq 49 20 DDE DDE NNP work_sny56posuffadh5a367n5vi4uq 49 21 , , , work_sny56posuffadh5a367n5vi4uq 49 22 which which WDT work_sny56posuffadh5a367n5vi4uq 49 23 contain contain VBP work_sny56posuffadh5a367n5vi4uq 49 24 an an DT work_sny56posuffadh5a367n5vi4uq 49 25 active active JJ work_sny56posuffadh5a367n5vi4uq 49 26 and and CC work_sny56posuffadh5a367n5vi4uq 49 27 inactive inactive JJ work_sny56posuffadh5a367n5vi4uq 49 28 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 49 29 gene gene NN work_sny56posuffadh5a367n5vi4uq 49 30 , , , work_sny56posuffadh5a367n5vi4uq 49 31 respectively respectively RB work_sny56posuffadh5a367n5vi4uq 49 32 , , , work_sny56posuffadh5a367n5vi4uq 49 33 and and CC work_sny56posuffadh5a367n5vi4uq 49 34 pT pT NNP work_sny56posuffadh5a367n5vi4uq 49 35 / / SYM work_sny56posuffadh5a367n5vi4uq 49 36 neo neo NNP work_sny56posuffadh5a367n5vi4uq 49 37 which which WDT work_sny56posuffadh5a367n5vi4uq 49 38 contains contain VBZ work_sny56posuffadh5a367n5vi4uq 49 39 a a DT work_sny56posuffadh5a367n5vi4uq 49 40 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 49 41 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 49 42 3 3 CD work_sny56posuffadh5a367n5vi4uq 49 43 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 49 44 as as RB work_sny56posuffadh5a367n5vi4uq 49 45 well well RB work_sny56posuffadh5a367n5vi4uq 49 46 as as IN work_sny56posuffadh5a367n5vi4uq 49 47 pT pT NNP work_sny56posuffadh5a367n5vi4uq 49 48 / / SYM work_sny56posuffadh5a367n5vi4uq 49 49 HindIIIneo HindIIIneo NNP work_sny56posuffadh5a367n5vi4uq 49 50 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 49 51 Adam Adam NNP work_sny56posuffadh5a367n5vi4uq 49 52 Dupuy Dupuy NNP work_sny56posuffadh5a367n5vi4uq 49 53 , , , work_sny56posuffadh5a367n5vi4uq 49 54 unpublished unpublished JJ work_sny56posuffadh5a367n5vi4uq 49 55 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 49 56 can can MD work_sny56posuffadh5a367n5vi4uq 49 57 be be VB work_sny56posuffadh5a367n5vi4uq 49 58 found find VBN work_sny56posuffadh5a367n5vi4uq 49 59 through through IN work_sny56posuffadh5a367n5vi4uq 49 60 our -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 49 61 website website NN work_sny56posuffadh5a367n5vi4uq 49 62 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 49 63 20 20 CD work_sny56posuffadh5a367n5vi4uq 49 64 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 49 65 . . . work_sny56posuffadh5a367n5vi4uq 50 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 50 2 name name VBP work_sny56posuffadh5a367n5vi4uq 50 3 pT pT NNP work_sny56posuffadh5a367n5vi4uq 50 4 / / SYM work_sny56posuffadh5a367n5vi4uq 50 5 HindIIIneo HindIIIneo NNP work_sny56posuffadh5a367n5vi4uq 50 6 variants variant NNS work_sny56posuffadh5a367n5vi4uq 50 7 by by IN work_sny56posuffadh5a367n5vi4uq 50 8 the the DT work_sny56posuffadh5a367n5vi4uq 50 9 DRs dr NNS work_sny56posuffadh5a367n5vi4uq 50 10 that that WDT work_sny56posuffadh5a367n5vi4uq 50 11 are be VBP work_sny56posuffadh5a367n5vi4uq 50 12 in in IN work_sny56posuffadh5a367n5vi4uq 50 13 their -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 50 14 left left NN work_sny56posuffadh5a367n5vi4uq 50 15 and and CC work_sny56posuffadh5a367n5vi4uq 50 16 right right JJ work_sny56posuffadh5a367n5vi4uq 50 17 IRs irs NNS work_sny56posuffadh5a367n5vi4uq 50 18 . . . work_sny56posuffadh5a367n5vi4uq 51 1 All all DT work_sny56posuffadh5a367n5vi4uq 51 2 variant variant JJ work_sny56posuffadh5a367n5vi4uq 51 3 pT pT NNP work_sny56posuffadh5a367n5vi4uq 51 4 / / SYM work_sny56posuffadh5a367n5vi4uq 51 5 HindIIIneo HindIIIneo NNP work_sny56posuffadh5a367n5vi4uq 51 6 constructs construct NNS work_sny56posuffadh5a367n5vi4uq 51 7 were be VBD work_sny56posuffadh5a367n5vi4uq 51 8 made make VBN work_sny56posuffadh5a367n5vi4uq 51 9 by by IN work_sny56posuffadh5a367n5vi4uq 51 10 PCR- PCR- NNP work_sny56posuffadh5a367n5vi4uq 51 11 mediated mediate VBN work_sny56posuffadh5a367n5vi4uq 51 12 , , , work_sny56posuffadh5a367n5vi4uq 51 13 site site NN work_sny56posuffadh5a367n5vi4uq 51 14 - - HYPH work_sny56posuffadh5a367n5vi4uq 51 15 directed direct VBN work_sny56posuffadh5a367n5vi4uq 51 16 mutagenesis mutagenesis NN work_sny56posuffadh5a367n5vi4uq 51 17 described describe VBN work_sny56posuffadh5a367n5vi4uq 51 18 previously previously RB work_sny56posuffadh5a367n5vi4uq 51 19 for for IN work_sny56posuffadh5a367n5vi4uq 51 20 LoLi LoLi NNP work_sny56posuffadh5a367n5vi4uq 51 21 – – : work_sny56posuffadh5a367n5vi4uq 51 22 LiLo LiLo NNP work_sny56posuffadh5a367n5vi4uq 51 23 , , , work_sny56posuffadh5a367n5vi4uq 51 24 RoRi RoRi NNP work_sny56posuffadh5a367n5vi4uq 51 25 – – : work_sny56posuffadh5a367n5vi4uq 51 26 RiRo RiRo NNP work_sny56posuffadh5a367n5vi4uq 51 27 , , , work_sny56posuffadh5a367n5vi4uq 51 28 LiLi LiLi NNP work_sny56posuffadh5a367n5vi4uq 51 29 – – : work_sny56posuffadh5a367n5vi4uq 51 30 RiRo RiRo NNP work_sny56posuffadh5a367n5vi4uq 51 31 and and CC work_sny56posuffadh5a367n5vi4uq 51 32 LoLo LoLo NNP work_sny56posuffadh5a367n5vi4uq 51 33 – – : work_sny56posuffadh5a367n5vi4uq 51 34 RiRo RiRo NNP work_sny56posuffadh5a367n5vi4uq 51 35 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 51 36 16 16 CD work_sny56posuffadh5a367n5vi4uq 51 37 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 51 38 . . . work_sny56posuffadh5a367n5vi4uq 52 1 Mutagenic mutagenic JJ work_sny56posuffadh5a367n5vi4uq 52 2 primers primer NNS work_sny56posuffadh5a367n5vi4uq 52 3 SacI SacI NNP work_sny56posuffadh5a367n5vi4uq 52 4 + + CC work_sny56posuffadh5a367n5vi4uq 52 5 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 52 6 and and CC work_sny56posuffadh5a367n5vi4uq 52 7 BamHI BamHI NNP work_sny56posuffadh5a367n5vi4uq 52 8 + + NNP work_sny56posuffadh5a367n5vi4uq 52 9 Ro Ro NNP work_sny56posuffadh5a367n5vi4uq 52 10 were be VBD work_sny56posuffadh5a367n5vi4uq 52 11 used use VBN work_sny56posuffadh5a367n5vi4uq 52 12 to to TO work_sny56posuffadh5a367n5vi4uq 52 13 amplify amplify VB work_sny56posuffadh5a367n5vi4uq 52 14 complete complete JJ work_sny56posuffadh5a367n5vi4uq 52 15 variant variant JJ work_sny56posuffadh5a367n5vi4uq 52 16 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 52 17 on on IN work_sny56posuffadh5a367n5vi4uq 52 18 pT pT NNP work_sny56posuffadh5a367n5vi4uq 52 19 / / SYM work_sny56posuffadh5a367n5vi4uq 52 20 HindIIIneo hindiiineo NN work_sny56posuffadh5a367n5vi4uq 52 21 . . . work_sny56posuffadh5a367n5vi4uq 53 1 After after IN work_sny56posuffadh5a367n5vi4uq 53 2 digesting digest VBG work_sny56posuffadh5a367n5vi4uq 53 3 with with IN work_sny56posuffadh5a367n5vi4uq 53 4 SacI SacI NNP work_sny56posuffadh5a367n5vi4uq 53 5 and and CC work_sny56posuffadh5a367n5vi4uq 53 6 BamHI BamHI NNP work_sny56posuffadh5a367n5vi4uq 53 7 , , , work_sny56posuffadh5a367n5vi4uq 53 8 the the DT work_sny56posuffadh5a367n5vi4uq 53 9 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 53 10 fragments fragment NNS work_sny56posuffadh5a367n5vi4uq 53 11 were be VBD work_sny56posuffadh5a367n5vi4uq 53 12 ligated ligate VBN work_sny56posuffadh5a367n5vi4uq 53 13 into into IN work_sny56posuffadh5a367n5vi4uq 53 14 the the DT work_sny56posuffadh5a367n5vi4uq 53 15 SacI SacI NNP work_sny56posuffadh5a367n5vi4uq 53 16 / / SYM work_sny56posuffadh5a367n5vi4uq 53 17 BamHI BamHI NNP work_sny56posuffadh5a367n5vi4uq 53 18 vector vector NN work_sny56posuffadh5a367n5vi4uq 53 19 fragment fragment NN work_sny56posuffadh5a367n5vi4uq 53 20 of of IN work_sny56posuffadh5a367n5vi4uq 53 21 pT pT NNP work_sny56posuffadh5a367n5vi4uq 53 22 / / SYM work_sny56posuffadh5a367n5vi4uq 53 23 HindIIIneo hindiiineo NN work_sny56posuffadh5a367n5vi4uq 53 24 . . . work_sny56posuffadh5a367n5vi4uq 54 1 All all DT work_sny56posuffadh5a367n5vi4uq 54 2 constructs construct NNS work_sny56posuffadh5a367n5vi4uq 54 3 were be VBD work_sny56posuffadh5a367n5vi4uq 54 4 confirmed confirm VBN work_sny56posuffadh5a367n5vi4uq 54 5 by by IN work_sny56posuffadh5a367n5vi4uq 54 6 sequencing sequence VBG work_sny56posuffadh5a367n5vi4uq 54 7 . . . work_sny56posuffadh5a367n5vi4uq 55 1 Mutagenic mutagenic JJ work_sny56posuffadh5a367n5vi4uq 55 2 primers primer NNS work_sny56posuffadh5a367n5vi4uq 55 3 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 55 4 used use VBD work_sny56posuffadh5a367n5vi4uq 55 5 are be VBP work_sny56posuffadh5a367n5vi4uq 55 6 listed list VBN work_sny56posuffadh5a367n5vi4uq 55 7 below below RB work_sny56posuffadh5a367n5vi4uq 55 8 with with IN work_sny56posuffadh5a367n5vi4uq 55 9 specific specific JJ work_sny56posuffadh5a367n5vi4uq 55 10 mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 55 11 underlined underline VBN work_sny56posuffadh5a367n5vi4uq 55 12 : : : work_sny56posuffadh5a367n5vi4uq 55 13 SacI SacI NNP work_sny56posuffadh5a367n5vi4uq 55 14 + + CC work_sny56posuffadh5a367n5vi4uq 55 15 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 55 16 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 55 17 AAA AAA NNP work_sny56posuffadh5a367n5vi4uq 55 18 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 55 19 : : : work_sny56posuffadh5a367n5vi4uq 55 20 TTGGAGCTCGGTACCCTAAAATTGAA- TTGGAGCTCGGTACCCTAAAATTGAA- VBZ work_sny56posuffadh5a367n5vi4uq 55 21 GTC GTC NNP work_sny56posuffadh5a367n5vi4uq 55 22 SacI SacI NNP work_sny56posuffadh5a367n5vi4uq 55 23 + + NNP work_sny56posuffadh5a367n5vi4uq 55 24 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 55 25 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 55 26 CAA CAA NNP work_sny56posuffadh5a367n5vi4uq 55 27 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 55 28 : : : work_sny56posuffadh5a367n5vi4uq 55 29 TTGGAGCTCGGTACCCTACAATTGAA- TTGGAGCTCGGTACCCTACAATTGAA- NNP work_sny56posuffadh5a367n5vi4uq 55 30 GTC GTC NNP work_sny56posuffadh5a367n5vi4uq 55 31 SacI SacI NNP work_sny56posuffadh5a367n5vi4uq 55 32 + + NNP work_sny56posuffadh5a367n5vi4uq 55 33 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 55 34 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 55 35 AAG AAG NNP work_sny56posuffadh5a367n5vi4uq 55 36 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 55 37 : : : work_sny56posuffadh5a367n5vi4uq 55 38 TTGGAGCTCGGTACCCTAAAGTTGAA- TTGGAGCTCGGTACCCTAAAGTTGAA- NNP work_sny56posuffadh5a367n5vi4uq 55 39 GTC GTC NNP work_sny56posuffadh5a367n5vi4uq 55 40 BamHI BamHI NNP work_sny56posuffadh5a367n5vi4uq 55 41 + + NNP work_sny56posuffadh5a367n5vi4uq 55 42 Ro Ro NNP work_sny56posuffadh5a367n5vi4uq 55 43 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 55 44 AAA AAA NNP work_sny56posuffadh5a367n5vi4uq 55 45 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 55 46 : : : work_sny56posuffadh5a367n5vi4uq 55 47 AGCTAGAGGATCCCCTAAAATTGA- agctagaggatcccctaaaattga- JJ work_sny56posuffadh5a367n5vi4uq 55 48 AGTCG agtcg NN work_sny56posuffadh5a367n5vi4uq 55 49 Copyright copyright NN work_sny56posuffadh5a367n5vi4uq 55 50   CD work_sny56posuffadh5a367n5vi4uq 55 51 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 55 52 John John NNP work_sny56posuffadh5a367n5vi4uq 55 53 Wiley Wiley NNP work_sny56posuffadh5a367n5vi4uq 55 54 & & CC work_sny56posuffadh5a367n5vi4uq 55 55 Sons Sons NNPS work_sny56posuffadh5a367n5vi4uq 55 56 , , , work_sny56posuffadh5a367n5vi4uq 55 57 Ltd. Ltd. NNP work_sny56posuffadh5a367n5vi4uq 55 58 J J NNP work_sny56posuffadh5a367n5vi4uq 55 59 Gene Gene NNP work_sny56posuffadh5a367n5vi4uq 55 60 Med Med NNP work_sny56posuffadh5a367n5vi4uq 55 61 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 55 62 ; ; : work_sny56posuffadh5a367n5vi4uq 55 63 6 6 CD work_sny56posuffadh5a367n5vi4uq 55 64 : : : work_sny56posuffadh5a367n5vi4uq 55 65 574 574 CD work_sny56posuffadh5a367n5vi4uq 55 66 – – : work_sny56posuffadh5a367n5vi4uq 55 67 583 583 CD work_sny56posuffadh5a367n5vi4uq 55 68 . . . work_sny56posuffadh5a367n5vi4uq 56 1 576 576 CD work_sny56posuffadh5a367n5vi4uq 56 2 G. G. NNP work_sny56posuffadh5a367n5vi4uq 56 3 Liu Liu NNP work_sny56posuffadh5a367n5vi4uq 56 4 et et NNP work_sny56posuffadh5a367n5vi4uq 56 5 al al NNP work_sny56posuffadh5a367n5vi4uq 56 6 . . . work_sny56posuffadh5a367n5vi4uq 57 1 BamHI BamHI NNP work_sny56posuffadh5a367n5vi4uq 57 2 + + NNP work_sny56posuffadh5a367n5vi4uq 57 3 Ro Ro NNP work_sny56posuffadh5a367n5vi4uq 57 4 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 57 5 CAA caa NN work_sny56posuffadh5a367n5vi4uq 57 6 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 57 7 : : : work_sny56posuffadh5a367n5vi4uq 57 8 AGCTAGAGGATCCCCTACAATTGA- AGCTAGAGGATCCCCTACAATTGA- NNP work_sny56posuffadh5a367n5vi4uq 57 9 AGTCG AGTCG NNP work_sny56posuffadh5a367n5vi4uq 57 10 BamHI BamHI NNP work_sny56posuffadh5a367n5vi4uq 57 11 + + NNP work_sny56posuffadh5a367n5vi4uq 57 12 Ro Ro NNP work_sny56posuffadh5a367n5vi4uq 57 13 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 57 14 AAG AAG NNP work_sny56posuffadh5a367n5vi4uq 57 15 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 57 16 : : : work_sny56posuffadh5a367n5vi4uq 57 17 AGCTAGAGGATCCCCTAAAGTTGA- agctagaggatcccctaaagttga- JJ work_sny56posuffadh5a367n5vi4uq 57 18 AGTCG AGTCG NNP work_sny56posuffadh5a367n5vi4uq 57 19 BamHI BamHI NNP work_sny56posuffadh5a367n5vi4uq 57 20 + + NNP work_sny56posuffadh5a367n5vi4uq 57 21 Ro Ro NNP work_sny56posuffadh5a367n5vi4uq 57 22 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 57 23 CC CC NNP work_sny56posuffadh5a367n5vi4uq 57 24 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 57 25 : : : work_sny56posuffadh5a367n5vi4uq 57 26 AGCTAGAGGATCCCCCCCAGTTGAA- AGCTAGAGGATCCCCCCCAGTTGAA- NNP work_sny56posuffadh5a367n5vi4uq 57 27 GTCG GTCG NNP work_sny56posuffadh5a367n5vi4uq 57 28 SacI SacI NNP work_sny56posuffadh5a367n5vi4uq 57 29 + + CC work_sny56posuffadh5a367n5vi4uq 57 30 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 57 31 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 57 32 GG GG NNP work_sny56posuffadh5a367n5vi4uq 57 33 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 57 34 : : : work_sny56posuffadh5a367n5vi4uq 57 35 TTGGAGCTCGGTACCCGGCAGTTGAA- TTGGAGCTCGGTACCCGGCAGTTGAA- NNP work_sny56posuffadh5a367n5vi4uq 57 36 GTC GTC NNP work_sny56posuffadh5a367n5vi4uq 57 37 Plasmid Plasmid NNP work_sny56posuffadh5a367n5vi4uq 57 38 - - HYPH work_sny56posuffadh5a367n5vi4uq 57 39 based base VBN work_sny56posuffadh5a367n5vi4uq 57 40 excision excision NN work_sny56posuffadh5a367n5vi4uq 57 41 analysis analysis NN work_sny56posuffadh5a367n5vi4uq 57 42 Excision excision NN work_sny56posuffadh5a367n5vi4uq 57 43 in in IN work_sny56posuffadh5a367n5vi4uq 57 44 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 57 45 cells cell NNS work_sny56posuffadh5a367n5vi4uq 57 46 : : : work_sny56posuffadh5a367n5vi4uq 57 47 500 500 CD work_sny56posuffadh5a367n5vi4uq 57 48 ng ng NNP work_sny56posuffadh5a367n5vi4uq 57 49 pT pT NNP work_sny56posuffadh5a367n5vi4uq 57 50 / / SYM work_sny56posuffadh5a367n5vi4uq 57 51 neo neo NNP work_sny56posuffadh5a367n5vi4uq 57 52 or or CC work_sny56posuffadh5a367n5vi4uq 57 53 pT pT NNP work_sny56posuffadh5a367n5vi4uq 57 54 / / SYM work_sny56posuffadh5a367n5vi4uq 57 55 HindIIIneo HindIIIneo NNP work_sny56posuffadh5a367n5vi4uq 57 56 mutation mutation NN work_sny56posuffadh5a367n5vi4uq 57 57 constructs construct NNS work_sny56posuffadh5a367n5vi4uq 57 58 were be VBD work_sny56posuffadh5a367n5vi4uq 57 59 co co VBN work_sny56posuffadh5a367n5vi4uq 57 60 - - VBN work_sny56posuffadh5a367n5vi4uq 57 61 transfected transfected JJ work_sny56posuffadh5a367n5vi4uq 57 62 with with IN work_sny56posuffadh5a367n5vi4uq 57 63 100 100 CD work_sny56posuffadh5a367n5vi4uq 57 64 ng ng NN work_sny56posuffadh5a367n5vi4uq 57 65 pCMVSB pcmvsb NN work_sny56posuffadh5a367n5vi4uq 57 66 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 57 67 or or CC work_sny56posuffadh5a367n5vi4uq 57 68 pSB10- pSB10- NNP work_sny56posuffadh5a367n5vi4uq 57 69 � � , work_sny56posuffadh5a367n5vi4uq 57 70 DDE DDE NNP work_sny56posuffadh5a367n5vi4uq 57 71 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 57 72 into into IN work_sny56posuffadh5a367n5vi4uq 57 73 3 3 CD work_sny56posuffadh5a367n5vi4uq 57 74 × × CD work_sny56posuffadh5a367n5vi4uq 57 75 105 105 CD work_sny56posuffadh5a367n5vi4uq 57 76 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 57 77 cells cell NNS work_sny56posuffadh5a367n5vi4uq 57 78 . . . work_sny56posuffadh5a367n5vi4uq 58 1 Four four CD work_sny56posuffadh5a367n5vi4uq 58 2 days day NNS work_sny56posuffadh5a367n5vi4uq 58 3 post post NN work_sny56posuffadh5a367n5vi4uq 58 4 - - JJ work_sny56posuffadh5a367n5vi4uq 58 5 transfection transfection JJ work_sny56posuffadh5a367n5vi4uq 58 6 , , , work_sny56posuffadh5a367n5vi4uq 58 7 cells cell NNS work_sny56posuffadh5a367n5vi4uq 58 8 were be VBD work_sny56posuffadh5a367n5vi4uq 58 9 collected collect VBN work_sny56posuffadh5a367n5vi4uq 58 10 and and CC work_sny56posuffadh5a367n5vi4uq 58 11 lysed lyse VBN work_sny56posuffadh5a367n5vi4uq 58 12 in in IN work_sny56posuffadh5a367n5vi4uq 58 13 200 200 CD work_sny56posuffadh5a367n5vi4uq 58 14 µl µl IN work_sny56posuffadh5a367n5vi4uq 58 15 lysis lysis NN work_sny56posuffadh5a367n5vi4uq 58 16 buffer buffer NN work_sny56posuffadh5a367n5vi4uq 58 17 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 58 18 50 50 CD work_sny56posuffadh5a367n5vi4uq 58 19 mM mM NNP work_sny56posuffadh5a367n5vi4uq 58 20 KCl KCl NNS work_sny56posuffadh5a367n5vi4uq 58 21 , , , work_sny56posuffadh5a367n5vi4uq 58 22 10 10 CD work_sny56posuffadh5a367n5vi4uq 58 23 mM mM NNP work_sny56posuffadh5a367n5vi4uq 58 24 Tris Tris NNP work_sny56posuffadh5a367n5vi4uq 58 25 - - HYPH work_sny56posuffadh5a367n5vi4uq 58 26 HCl HCl NNP work_sny56posuffadh5a367n5vi4uq 58 27 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 58 28 pH pH NNP work_sny56posuffadh5a367n5vi4uq 58 29 8.3 8.3 CD work_sny56posuffadh5a367n5vi4uq 58 30 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 58 31 , , , work_sny56posuffadh5a367n5vi4uq 58 32 2.5 2.5 CD work_sny56posuffadh5a367n5vi4uq 58 33 mM mM NNP work_sny56posuffadh5a367n5vi4uq 58 34 MgCl2 mgcl2 NN work_sny56posuffadh5a367n5vi4uq 58 35 , , , work_sny56posuffadh5a367n5vi4uq 58 36 10 10 CD work_sny56posuffadh5a367n5vi4uq 58 37 mM mm NN work_sny56posuffadh5a367n5vi4uq 58 38 EDTA EDTA NNP work_sny56posuffadh5a367n5vi4uq 58 39 , , , work_sny56posuffadh5a367n5vi4uq 58 40 0.45 0.45 CD work_sny56posuffadh5a367n5vi4uq 58 41 % % NN work_sny56posuffadh5a367n5vi4uq 58 42 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 58 43 w w NNP work_sny56posuffadh5a367n5vi4uq 58 44 / / SYM work_sny56posuffadh5a367n5vi4uq 58 45 v v NNP work_sny56posuffadh5a367n5vi4uq 58 46 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 58 47 NP40 NP40 NNP work_sny56posuffadh5a367n5vi4uq 58 48 , , , work_sny56posuffadh5a367n5vi4uq 58 49 0.45 0.45 CD work_sny56posuffadh5a367n5vi4uq 58 50 % % NN work_sny56posuffadh5a367n5vi4uq 58 51 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 58 52 w w NNP work_sny56posuffadh5a367n5vi4uq 58 53 / / SYM work_sny56posuffadh5a367n5vi4uq 58 54 v v NNP work_sny56posuffadh5a367n5vi4uq 58 55 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 58 56 Tween-20 tween-20 CD work_sny56posuffadh5a367n5vi4uq 58 57 , , , work_sny56posuffadh5a367n5vi4uq 58 58 100 100 CD work_sny56posuffadh5a367n5vi4uq 58 59 µg µg NN work_sny56posuffadh5a367n5vi4uq 58 60 / / SYM work_sny56posuffadh5a367n5vi4uq 58 61 ml ml NNP work_sny56posuffadh5a367n5vi4uq 58 62 Proteinase Proteinase NNP work_sny56posuffadh5a367n5vi4uq 58 63 K K NNP work_sny56posuffadh5a367n5vi4uq 58 64 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 58 65 and and CC work_sny56posuffadh5a367n5vi4uq 58 66 incubated incubate VBD work_sny56posuffadh5a367n5vi4uq 58 67 at at IN work_sny56posuffadh5a367n5vi4uq 58 68 55 55 CD work_sny56posuffadh5a367n5vi4uq 58 69 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 58 70 C C NNP work_sny56posuffadh5a367n5vi4uq 58 71 for for IN work_sny56posuffadh5a367n5vi4uq 58 72 2 2 CD work_sny56posuffadh5a367n5vi4uq 58 73 – – SYM work_sny56posuffadh5a367n5vi4uq 58 74 3 3 CD work_sny56posuffadh5a367n5vi4uq 58 75 h h NN work_sny56posuffadh5a367n5vi4uq 58 76 followed follow VBN work_sny56posuffadh5a367n5vi4uq 58 77 by by IN work_sny56posuffadh5a367n5vi4uq 58 78 95 95 CD work_sny56posuffadh5a367n5vi4uq 58 79 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 58 80 C C NNP work_sny56posuffadh5a367n5vi4uq 58 81 for for IN work_sny56posuffadh5a367n5vi4uq 58 82 20 20 CD work_sny56posuffadh5a367n5vi4uq 58 83 min min NN work_sny56posuffadh5a367n5vi4uq 58 84 to to TO work_sny56posuffadh5a367n5vi4uq 58 85 inactivate inactivate VB work_sny56posuffadh5a367n5vi4uq 58 86 the the DT work_sny56posuffadh5a367n5vi4uq 58 87 Proteinase Proteinase NNP work_sny56posuffadh5a367n5vi4uq 58 88 K. K. NNP work_sny56posuffadh5a367n5vi4uq 58 89 For for IN work_sny56posuffadh5a367n5vi4uq 58 90 temporal temporal JJ work_sny56posuffadh5a367n5vi4uq 58 91 analyses analysis NNS work_sny56posuffadh5a367n5vi4uq 58 92 , , , work_sny56posuffadh5a367n5vi4uq 58 93 5 5 CD work_sny56posuffadh5a367n5vi4uq 58 94 µl µl IN work_sny56posuffadh5a367n5vi4uq 58 95 of of IN work_sny56posuffadh5a367n5vi4uq 58 96 lysate lysate NN work_sny56posuffadh5a367n5vi4uq 58 97 were be VBD work_sny56posuffadh5a367n5vi4uq 58 98 added add VBN work_sny56posuffadh5a367n5vi4uq 58 99 to to IN work_sny56posuffadh5a367n5vi4uq 58 100 the the DT work_sny56posuffadh5a367n5vi4uq 58 101 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 58 102 mixture mixture NN work_sny56posuffadh5a367n5vi4uq 58 103 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 58 104 1 1 CD work_sny56posuffadh5a367n5vi4uq 58 105 × × NN work_sny56posuffadh5a367n5vi4uq 58 106 NH4 NH4 NNP work_sny56posuffadh5a367n5vi4uq 58 107 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 58 108 buffer buffer NN work_sny56posuffadh5a367n5vi4uq 58 109 , , , work_sny56posuffadh5a367n5vi4uq 58 110 3 3 CD work_sny56posuffadh5a367n5vi4uq 58 111 mM mM NNP work_sny56posuffadh5a367n5vi4uq 58 112 MgCl2 mgcl2 NN work_sny56posuffadh5a367n5vi4uq 58 113 , , , work_sny56posuffadh5a367n5vi4uq 58 114 0.2 0.2 CD work_sny56posuffadh5a367n5vi4uq 58 115 mM mm NN work_sny56posuffadh5a367n5vi4uq 58 116 dNTP dntp NN work_sny56posuffadh5a367n5vi4uq 58 117 , , , work_sny56posuffadh5a367n5vi4uq 58 118 10 10 CD work_sny56posuffadh5a367n5vi4uq 58 119 pmol pmol NN work_sny56posuffadh5a367n5vi4uq 58 120 forward forward RB work_sny56posuffadh5a367n5vi4uq 58 121 and and CC work_sny56posuffadh5a367n5vi4uq 58 122 reverse reverse VBP work_sny56posuffadh5a367n5vi4uq 58 123 primer primer NN work_sny56posuffadh5a367n5vi4uq 58 124 each each DT work_sny56posuffadh5a367n5vi4uq 58 125 , , , work_sny56posuffadh5a367n5vi4uq 58 126 0.5 0.5 CD work_sny56posuffadh5a367n5vi4uq 58 127 µl µl IN work_sny56posuffadh5a367n5vi4uq 58 128 Biolase Biolase NNP work_sny56posuffadh5a367n5vi4uq 58 129 , , , work_sny56posuffadh5a367n5vi4uq 58 130 buffer buffer NN work_sny56posuffadh5a367n5vi4uq 58 131 and and CC work_sny56posuffadh5a367n5vi4uq 58 132 enzyme enzyme NNS work_sny56posuffadh5a367n5vi4uq 58 133 from from IN work_sny56posuffadh5a367n5vi4uq 58 134 Bioline Bioline NNP work_sny56posuffadh5a367n5vi4uq 58 135 USA USA NNP work_sny56posuffadh5a367n5vi4uq 58 136 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 58 137 . . . work_sny56posuffadh5a367n5vi4uq 59 1 The the DT work_sny56posuffadh5a367n5vi4uq 59 2 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 59 3 conditions condition NNS work_sny56posuffadh5a367n5vi4uq 59 4 were be VBD work_sny56posuffadh5a367n5vi4uq 59 5 as as IN work_sny56posuffadh5a367n5vi4uq 59 6 follows follow VBZ work_sny56posuffadh5a367n5vi4uq 59 7 : : : work_sny56posuffadh5a367n5vi4uq 59 8 94 94 CD work_sny56posuffadh5a367n5vi4uq 59 9 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 59 10 C C NNP work_sny56posuffadh5a367n5vi4uq 59 11 for for IN work_sny56posuffadh5a367n5vi4uq 59 12 5 5 CD work_sny56posuffadh5a367n5vi4uq 59 13 min min NN work_sny56posuffadh5a367n5vi4uq 59 14 , , , work_sny56posuffadh5a367n5vi4uq 59 15 60 60 CD work_sny56posuffadh5a367n5vi4uq 59 16 cycles cycle NNS work_sny56posuffadh5a367n5vi4uq 59 17 of of IN work_sny56posuffadh5a367n5vi4uq 59 18 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 59 19 94 94 CD work_sny56posuffadh5a367n5vi4uq 59 20 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 59 21 C C NNP work_sny56posuffadh5a367n5vi4uq 59 22 30 30 CD work_sny56posuffadh5a367n5vi4uq 59 23 s s NNP work_sny56posuffadh5a367n5vi4uq 59 24 , , , work_sny56posuffadh5a367n5vi4uq 59 25 64 64 CD work_sny56posuffadh5a367n5vi4uq 59 26 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 59 27 C C NNP work_sny56posuffadh5a367n5vi4uq 59 28 30 30 CD work_sny56posuffadh5a367n5vi4uq 59 29 s s NN work_sny56posuffadh5a367n5vi4uq 59 30 , , , work_sny56posuffadh5a367n5vi4uq 59 31 72 72 CD work_sny56posuffadh5a367n5vi4uq 59 32 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 59 33 C C NNP work_sny56posuffadh5a367n5vi4uq 59 34 20 20 CD work_sny56posuffadh5a367n5vi4uq 59 35 s s NNPS work_sny56posuffadh5a367n5vi4uq 59 36 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 59 37 , , , work_sny56posuffadh5a367n5vi4uq 59 38 followed follow VBN work_sny56posuffadh5a367n5vi4uq 59 39 by by IN work_sny56posuffadh5a367n5vi4uq 59 40 72 72 CD work_sny56posuffadh5a367n5vi4uq 59 41 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 59 42 C C NNP work_sny56posuffadh5a367n5vi4uq 59 43 for for IN work_sny56posuffadh5a367n5vi4uq 59 44 10 10 CD work_sny56posuffadh5a367n5vi4uq 59 45 min min NN work_sny56posuffadh5a367n5vi4uq 59 46 . . . work_sny56posuffadh5a367n5vi4uq 60 1 Five five CD work_sny56posuffadh5a367n5vi4uq 60 2 µl µl IN work_sny56posuffadh5a367n5vi4uq 60 3 of of IN work_sny56posuffadh5a367n5vi4uq 60 4 1/50 1/50 CD work_sny56posuffadh5a367n5vi4uq 60 5 dilution dilution NN work_sny56posuffadh5a367n5vi4uq 60 6 of of IN work_sny56posuffadh5a367n5vi4uq 60 7 the the DT work_sny56posuffadh5a367n5vi4uq 60 8 above above JJ work_sny56posuffadh5a367n5vi4uq 60 9 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 60 10 product product NN work_sny56posuffadh5a367n5vi4uq 60 11 were be VBD work_sny56posuffadh5a367n5vi4uq 60 12 used use VBN work_sny56posuffadh5a367n5vi4uq 60 13 for for IN work_sny56posuffadh5a367n5vi4uq 60 14 the the DT work_sny56posuffadh5a367n5vi4uq 60 15 second second JJ work_sny56posuffadh5a367n5vi4uq 60 16 round round NN work_sny56posuffadh5a367n5vi4uq 60 17 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 60 18 using use VBG work_sny56posuffadh5a367n5vi4uq 60 19 nested nested JJ work_sny56posuffadh5a367n5vi4uq 60 20 primers primer NNS work_sny56posuffadh5a367n5vi4uq 60 21 and and CC work_sny56posuffadh5a367n5vi4uq 60 22 1.5 1.5 CD work_sny56posuffadh5a367n5vi4uq 60 23 mM mM NNP work_sny56posuffadh5a367n5vi4uq 60 24 MgCl2 mgcl2 NN work_sny56posuffadh5a367n5vi4uq 60 25 ; ; : work_sny56posuffadh5a367n5vi4uq 60 26 the the DT work_sny56posuffadh5a367n5vi4uq 60 27 composition composition NN work_sny56posuffadh5a367n5vi4uq 60 28 of of IN work_sny56posuffadh5a367n5vi4uq 60 29 the the DT work_sny56posuffadh5a367n5vi4uq 60 30 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 60 31 mixture mixture NN work_sny56posuffadh5a367n5vi4uq 60 32 was be VBD work_sny56posuffadh5a367n5vi4uq 60 33 otherwise otherwise RB work_sny56posuffadh5a367n5vi4uq 60 34 the the DT work_sny56posuffadh5a367n5vi4uq 60 35 same same JJ work_sny56posuffadh5a367n5vi4uq 60 36 . . . work_sny56posuffadh5a367n5vi4uq 61 1 Nested Nested NNP work_sny56posuffadh5a367n5vi4uq 61 2 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 61 3 was be VBD work_sny56posuffadh5a367n5vi4uq 61 4 performed perform VBN work_sny56posuffadh5a367n5vi4uq 61 5 at at IN work_sny56posuffadh5a367n5vi4uq 61 6 94 94 CD work_sny56posuffadh5a367n5vi4uq 61 7 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 61 8 C C NNP work_sny56posuffadh5a367n5vi4uq 61 9 for for IN work_sny56posuffadh5a367n5vi4uq 61 10 5 5 CD work_sny56posuffadh5a367n5vi4uq 61 11 min min NN work_sny56posuffadh5a367n5vi4uq 61 12 , , , work_sny56posuffadh5a367n5vi4uq 61 13 35 35 CD work_sny56posuffadh5a367n5vi4uq 61 14 cycles cycle NNS work_sny56posuffadh5a367n5vi4uq 61 15 of of IN work_sny56posuffadh5a367n5vi4uq 61 16 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 61 17 94 94 CD work_sny56posuffadh5a367n5vi4uq 61 18 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 61 19 C C NNP work_sny56posuffadh5a367n5vi4uq 61 20 30 30 CD work_sny56posuffadh5a367n5vi4uq 61 21 s s NNP work_sny56posuffadh5a367n5vi4uq 61 22 , , , work_sny56posuffadh5a367n5vi4uq 61 23 64 64 CD work_sny56posuffadh5a367n5vi4uq 61 24 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 61 25 C C NNP work_sny56posuffadh5a367n5vi4uq 61 26 30 30 CD work_sny56posuffadh5a367n5vi4uq 61 27 s s NN work_sny56posuffadh5a367n5vi4uq 61 28 , , , work_sny56posuffadh5a367n5vi4uq 61 29 72 72 CD work_sny56posuffadh5a367n5vi4uq 61 30 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 61 31 C C NNP work_sny56posuffadh5a367n5vi4uq 61 32 5 5 CD work_sny56posuffadh5a367n5vi4uq 61 33 s s NNP work_sny56posuffadh5a367n5vi4uq 61 34 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 61 35 , , , work_sny56posuffadh5a367n5vi4uq 61 36 followed follow VBN work_sny56posuffadh5a367n5vi4uq 61 37 by by IN work_sny56posuffadh5a367n5vi4uq 61 38 72 72 CD work_sny56posuffadh5a367n5vi4uq 61 39 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 61 40 C C NNP work_sny56posuffadh5a367n5vi4uq 61 41 for for IN work_sny56posuffadh5a367n5vi4uq 61 42 10 10 CD work_sny56posuffadh5a367n5vi4uq 61 43 min min NN work_sny56posuffadh5a367n5vi4uq 61 44 . . . work_sny56posuffadh5a367n5vi4uq 62 1 For for IN work_sny56posuffadh5a367n5vi4uq 62 2 pT pT NNP work_sny56posuffadh5a367n5vi4uq 62 3 / / SYM work_sny56posuffadh5a367n5vi4uq 62 4 neo neo NNP work_sny56posuffadh5a367n5vi4uq 62 5 , , , work_sny56posuffadh5a367n5vi4uq 62 6 only only RB work_sny56posuffadh5a367n5vi4uq 62 7 one one CD work_sny56posuffadh5a367n5vi4uq 62 8 round round NN work_sny56posuffadh5a367n5vi4uq 62 9 of of IN work_sny56posuffadh5a367n5vi4uq 62 10 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 62 11 was be VBD work_sny56posuffadh5a367n5vi4uq 62 12 performed perform VBN work_sny56posuffadh5a367n5vi4uq 62 13 and and CC work_sny56posuffadh5a367n5vi4uq 62 14 gave give VBD work_sny56posuffadh5a367n5vi4uq 62 15 products product NNS work_sny56posuffadh5a367n5vi4uq 62 16 of of IN work_sny56posuffadh5a367n5vi4uq 62 17 about about RB work_sny56posuffadh5a367n5vi4uq 62 18 582 582 CD work_sny56posuffadh5a367n5vi4uq 62 19 bp bp NN work_sny56posuffadh5a367n5vi4uq 62 20 . . . work_sny56posuffadh5a367n5vi4uq 63 1 Primers primer NNS work_sny56posuffadh5a367n5vi4uq 63 2 were be VBD work_sny56posuffadh5a367n5vi4uq 63 3 : : : work_sny56posuffadh5a367n5vi4uq 63 4 o o IN work_sny56posuffadh5a367n5vi4uq 63 5 - - HYPH work_sny56posuffadh5a367n5vi4uq 63 6 lac lac NNP work_sny56posuffadh5a367n5vi4uq 63 7 - - HYPH work_sny56posuffadh5a367n5vi4uq 63 8 L l NN work_sny56posuffadh5a367n5vi4uq 63 9 : : : work_sny56posuffadh5a367n5vi4uq 63 10 5′-GGCTGGCTTAACTATGCGGCATCAG-3′ 5′-ggctggcttaactatgcggcatcag-3′ CD work_sny56posuffadh5a367n5vi4uq 63 11 o o NN work_sny56posuffadh5a367n5vi4uq 63 12 - - HYPH work_sny56posuffadh5a367n5vi4uq 63 13 lac lac NN work_sny56posuffadh5a367n5vi4uq 63 14 - - HYPH work_sny56posuffadh5a367n5vi4uq 63 15 R r NN work_sny56posuffadh5a367n5vi4uq 63 16 : : : work_sny56posuffadh5a367n5vi4uq 63 17 5′-GTCAGTGAGCGAGGAAGCGGAAGAG-3′ 5′-GTCAGTGAGCGAGGAAGCGGAAGAG-3′ NNP work_sny56posuffadh5a367n5vi4uq 63 18 For for IN work_sny56posuffadh5a367n5vi4uq 63 19 pT pT NNP work_sny56posuffadh5a367n5vi4uq 63 20 / / SYM work_sny56posuffadh5a367n5vi4uq 63 21 HindIIIneo HindIIIneo NNP work_sny56posuffadh5a367n5vi4uq 63 22 and and CC work_sny56posuffadh5a367n5vi4uq 63 23 its -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 63 24 mutation mutation NN work_sny56posuffadh5a367n5vi4uq 63 25 constructs construct NNS work_sny56posuffadh5a367n5vi4uq 63 26 , , , work_sny56posuffadh5a367n5vi4uq 63 27 nested nest VBN work_sny56posuffadh5a367n5vi4uq 63 28 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 63 29 gave give VBD work_sny56posuffadh5a367n5vi4uq 63 30 products product NNS work_sny56posuffadh5a367n5vi4uq 63 31 of of IN work_sny56posuffadh5a367n5vi4uq 63 32 about about RB work_sny56posuffadh5a367n5vi4uq 63 33 316 316 CD work_sny56posuffadh5a367n5vi4uq 63 34 bp bp NN work_sny56posuffadh5a367n5vi4uq 63 35 . . . work_sny56posuffadh5a367n5vi4uq 64 1 Primers primer NNS work_sny56posuffadh5a367n5vi4uq 64 2 were be VBD work_sny56posuffadh5a367n5vi4uq 64 3 : : : work_sny56posuffadh5a367n5vi4uq 64 4 1st 1st JJ work_sny56posuffadh5a367n5vi4uq 64 5 round round NN work_sny56posuffadh5a367n5vi4uq 64 6 : : : work_sny56posuffadh5a367n5vi4uq 64 7 F1-ex F1-ex NNS work_sny56posuffadh5a367n5vi4uq 64 8 : : : work_sny56posuffadh5a367n5vi4uq 64 9 5′-CCAAACTGGAACAACACTCAAC- 5′-ccaaactggaacaacactcaac- CD work_sny56posuffadh5a367n5vi4uq 64 10 CCTATCTC-3′ CCTATCTC-3′ NNP work_sny56posuffadh5a367n5vi4uq 64 11 o o NN work_sny56posuffadh5a367n5vi4uq 64 12 - - HYPH work_sny56posuffadh5a367n5vi4uq 64 13 lac lac NN work_sny56posuffadh5a367n5vi4uq 64 14 - - HYPH work_sny56posuffadh5a367n5vi4uq 64 15 R r NN work_sny56posuffadh5a367n5vi4uq 64 16 : : : work_sny56posuffadh5a367n5vi4uq 64 17 5′-GTCAGTGAGCGAGGAAGCGGAAGAG-3′. 5′-gtcagtgagcgaggaagcggaagag-3′. CD work_sny56posuffadh5a367n5vi4uq 65 1 2nd 2nd JJ work_sny56posuffadh5a367n5vi4uq 65 2 round round NN work_sny56posuffadh5a367n5vi4uq 65 3 : : : work_sny56posuffadh5a367n5vi4uq 65 4 KJC031 KJC031 NNP work_sny56posuffadh5a367n5vi4uq 65 5 : : : work_sny56posuffadh5a367n5vi4uq 65 6 5′-CGATTAAGTTGGGTAACGCCAG- 5′-cgattaagttgggtaacgccag- CD work_sny56posuffadh5a367n5vi4uq 65 7 GGTTT-3′ GGTTT-3′ NNPS work_sny56posuffadh5a367n5vi4uq 65 8 i i PRP work_sny56posuffadh5a367n5vi4uq 65 9 - - HYPH work_sny56posuffadh5a367n5vi4uq 65 10 lac lac NN work_sny56posuffadh5a367n5vi4uq 65 11 - - HYPH work_sny56posuffadh5a367n5vi4uq 65 12 R r NN work_sny56posuffadh5a367n5vi4uq 65 13 : : : work_sny56posuffadh5a367n5vi4uq 65 14 5′-AGCTCACTCATTAGGCACCCCAGGC-3′. 5′-agctcactcattaggcaccccaggc-3′. LS work_sny56posuffadh5a367n5vi4uq 66 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 66 2 in in IN work_sny56posuffadh5a367n5vi4uq 66 3 zebrafish zebrafish NNP work_sny56posuffadh5a367n5vi4uq 66 4 embryos embryos NNP work_sny56posuffadh5a367n5vi4uq 66 5 : : : work_sny56posuffadh5a367n5vi4uq 66 6 25 25 CD work_sny56posuffadh5a367n5vi4uq 66 7 ng/µl ng/µl CD work_sny56posuffadh5a367n5vi4uq 66 8 pT pT NNP work_sny56posuffadh5a367n5vi4uq 66 9 / / SYM work_sny56posuffadh5a367n5vi4uq 66 10 neo neo NNP work_sny56posuffadh5a367n5vi4uq 66 11 was be VBD work_sny56posuffadh5a367n5vi4uq 66 12 co co VBN work_sny56posuffadh5a367n5vi4uq 66 13 - - VBN work_sny56posuffadh5a367n5vi4uq 66 14 injected inject VBN work_sny56posuffadh5a367n5vi4uq 66 15 with with IN work_sny56posuffadh5a367n5vi4uq 66 16 25 25 CD work_sny56posuffadh5a367n5vi4uq 66 17 ng/µl ng/µl CD work_sny56posuffadh5a367n5vi4uq 66 18 SB SB NNP work_sny56posuffadh5a367n5vi4uq 66 19 mRNA mrna NN work_sny56posuffadh5a367n5vi4uq 66 20 into into IN work_sny56posuffadh5a367n5vi4uq 66 21 one one CD work_sny56posuffadh5a367n5vi4uq 66 22 - - HYPH work_sny56posuffadh5a367n5vi4uq 66 23 cell cell NN work_sny56posuffadh5a367n5vi4uq 66 24 - - HYPH work_sny56posuffadh5a367n5vi4uq 66 25 stage stage NN work_sny56posuffadh5a367n5vi4uq 66 26 zebrafish zebrafish JJ work_sny56posuffadh5a367n5vi4uq 66 27 embryos embryo NNS work_sny56posuffadh5a367n5vi4uq 66 28 . . . work_sny56posuffadh5a367n5vi4uq 67 1 SB SB NNP work_sny56posuffadh5a367n5vi4uq 67 2 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 67 3 mRNA mRNA NNP work_sny56posuffadh5a367n5vi4uq 67 4 was be VBD work_sny56posuffadh5a367n5vi4uq 67 5 synthesized synthesize VBN work_sny56posuffadh5a367n5vi4uq 67 6 by by IN work_sny56posuffadh5a367n5vi4uq 67 7 in in IN work_sny56posuffadh5a367n5vi4uq 67 8 vitro vitro FW work_sny56posuffadh5a367n5vi4uq 67 9 transcription transcription NN work_sny56posuffadh5a367n5vi4uq 67 10 using use VBG work_sny56posuffadh5a367n5vi4uq 67 11 the the DT work_sny56posuffadh5a367n5vi4uq 67 12 Message Message NNP work_sny56posuffadh5a367n5vi4uq 67 13 Machine Machine NNP work_sny56posuffadh5a367n5vi4uq 67 14 large- large- NN work_sny56posuffadh5a367n5vi4uq 67 15 scale scale NN work_sny56posuffadh5a367n5vi4uq 67 16 in in IN work_sny56posuffadh5a367n5vi4uq 67 17 vitro vitro FW work_sny56posuffadh5a367n5vi4uq 67 18 transcription transcription NN work_sny56posuffadh5a367n5vi4uq 67 19 kit kit NN work_sny56posuffadh5a367n5vi4uq 67 20 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 67 21 Ambion Ambion NNP work_sny56posuffadh5a367n5vi4uq 67 22 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 67 23 . . . work_sny56posuffadh5a367n5vi4uq 68 1 The the DT work_sny56posuffadh5a367n5vi4uq 68 2 injection injection NN work_sny56posuffadh5a367n5vi4uq 68 3 volume volume NN work_sny56posuffadh5a367n5vi4uq 68 4 for for IN work_sny56posuffadh5a367n5vi4uq 68 5 each each DT work_sny56posuffadh5a367n5vi4uq 68 6 embryo embryo NN work_sny56posuffadh5a367n5vi4uq 68 7 was be VBD work_sny56posuffadh5a367n5vi4uq 68 8 1 1 CD work_sny56posuffadh5a367n5vi4uq 68 9 – – SYM work_sny56posuffadh5a367n5vi4uq 68 10 3 3 CD work_sny56posuffadh5a367n5vi4uq 68 11 nl nl NN work_sny56posuffadh5a367n5vi4uq 68 12 . . . work_sny56posuffadh5a367n5vi4uq 69 1 Twenty twenty CD work_sny56posuffadh5a367n5vi4uq 69 2 - - HYPH work_sny56posuffadh5a367n5vi4uq 69 3 four four CD work_sny56posuffadh5a367n5vi4uq 69 4 hours hour NNS work_sny56posuffadh5a367n5vi4uq 69 5 after after IN work_sny56posuffadh5a367n5vi4uq 69 6 injection injection NN work_sny56posuffadh5a367n5vi4uq 69 7 , , , work_sny56posuffadh5a367n5vi4uq 69 8 single single JJ work_sny56posuffadh5a367n5vi4uq 69 9 embryos embryo NNS work_sny56posuffadh5a367n5vi4uq 69 10 were be VBD work_sny56posuffadh5a367n5vi4uq 69 11 lysed lyse VBN work_sny56posuffadh5a367n5vi4uq 69 12 in in IN work_sny56posuffadh5a367n5vi4uq 69 13 50 50 CD work_sny56posuffadh5a367n5vi4uq 69 14 µl µl IN work_sny56posuffadh5a367n5vi4uq 69 15 lysis lysis NN work_sny56posuffadh5a367n5vi4uq 69 16 buffer buffer NN work_sny56posuffadh5a367n5vi4uq 69 17 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 69 18 10 10 CD work_sny56posuffadh5a367n5vi4uq 69 19 mM mm NN work_sny56posuffadh5a367n5vi4uq 69 20 EDTA edta NN work_sny56posuffadh5a367n5vi4uq 69 21 , , , work_sny56posuffadh5a367n5vi4uq 69 22 10 10 CD work_sny56posuffadh5a367n5vi4uq 69 23 mM mM NNP work_sny56posuffadh5a367n5vi4uq 69 24 Tris Tris NNP work_sny56posuffadh5a367n5vi4uq 69 25 - - HYPH work_sny56posuffadh5a367n5vi4uq 69 26 HCl HCl NNP work_sny56posuffadh5a367n5vi4uq 69 27 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 69 28 pH pH NNP work_sny56posuffadh5a367n5vi4uq 69 29 8.0 8.0 CD work_sny56posuffadh5a367n5vi4uq 69 30 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 69 31 , , , work_sny56posuffadh5a367n5vi4uq 69 32 200 200 CD work_sny56posuffadh5a367n5vi4uq 69 33 µg µg IN work_sny56posuffadh5a367n5vi4uq 69 34 / / SYM work_sny56posuffadh5a367n5vi4uq 69 35 ml ml NNP work_sny56posuffadh5a367n5vi4uq 69 36 Proteinase Proteinase NNP work_sny56posuffadh5a367n5vi4uq 69 37 K K NNP work_sny56posuffadh5a367n5vi4uq 69 38 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 69 39 as as IN work_sny56posuffadh5a367n5vi4uq 69 40 described describe VBN work_sny56posuffadh5a367n5vi4uq 69 41 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 69 42 18,19 18,19 CD work_sny56posuffadh5a367n5vi4uq 69 43 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 69 44 for for IN work_sny56posuffadh5a367n5vi4uq 69 45 3 3 CD work_sny56posuffadh5a367n5vi4uq 69 46 h h NN work_sny56posuffadh5a367n5vi4uq 69 47 at at IN work_sny56posuffadh5a367n5vi4uq 69 48 50 50 CD work_sny56posuffadh5a367n5vi4uq 69 49 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 69 50 C C NNP work_sny56posuffadh5a367n5vi4uq 69 51 , , , work_sny56posuffadh5a367n5vi4uq 69 52 followed follow VBN work_sny56posuffadh5a367n5vi4uq 69 53 by by IN work_sny56posuffadh5a367n5vi4uq 69 54 20 20 CD work_sny56posuffadh5a367n5vi4uq 69 55 min min NN work_sny56posuffadh5a367n5vi4uq 69 56 incubation incubation NN work_sny56posuffadh5a367n5vi4uq 69 57 at at IN work_sny56posuffadh5a367n5vi4uq 69 58 95 95 CD work_sny56posuffadh5a367n5vi4uq 69 59 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 69 60 C C NNP work_sny56posuffadh5a367n5vi4uq 69 61 to to TO work_sny56posuffadh5a367n5vi4uq 69 62 inactivate inactivate VB work_sny56posuffadh5a367n5vi4uq 69 63 the the DT work_sny56posuffadh5a367n5vi4uq 69 64 Proteinase Proteinase NNP work_sny56posuffadh5a367n5vi4uq 69 65 K K NNP work_sny56posuffadh5a367n5vi4uq 69 66 ; ; : work_sny56posuffadh5a367n5vi4uq 69 67 2 2 CD work_sny56posuffadh5a367n5vi4uq 69 68 µl µl XX work_sny56posuffadh5a367n5vi4uq 69 69 of of IN work_sny56posuffadh5a367n5vi4uq 69 70 embryo embryo NN work_sny56posuffadh5a367n5vi4uq 69 71 lysate lysate NN work_sny56posuffadh5a367n5vi4uq 69 72 were be VBD work_sny56posuffadh5a367n5vi4uq 69 73 used use VBN work_sny56posuffadh5a367n5vi4uq 69 74 for for IN work_sny56posuffadh5a367n5vi4uq 69 75 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 69 76 . . . work_sny56posuffadh5a367n5vi4uq 70 1 The the DT work_sny56posuffadh5a367n5vi4uq 70 2 program program NN work_sny56posuffadh5a367n5vi4uq 70 3 used use VBN work_sny56posuffadh5a367n5vi4uq 70 4 for for IN work_sny56posuffadh5a367n5vi4uq 70 5 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 70 6 was be VBD work_sny56posuffadh5a367n5vi4uq 70 7 as as IN work_sny56posuffadh5a367n5vi4uq 70 8 follows follow VBZ work_sny56posuffadh5a367n5vi4uq 70 9 : : : work_sny56posuffadh5a367n5vi4uq 70 10 94 94 CD work_sny56posuffadh5a367n5vi4uq 70 11 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 70 12 C C NNP work_sny56posuffadh5a367n5vi4uq 70 13 5 5 CD work_sny56posuffadh5a367n5vi4uq 70 14 min min NN work_sny56posuffadh5a367n5vi4uq 70 15 , , , work_sny56posuffadh5a367n5vi4uq 70 16 30 30 CD work_sny56posuffadh5a367n5vi4uq 70 17 cycles cycle NNS work_sny56posuffadh5a367n5vi4uq 70 18 of of IN work_sny56posuffadh5a367n5vi4uq 70 19 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 70 20 94 94 CD work_sny56posuffadh5a367n5vi4uq 70 21 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 70 22 C C NNP work_sny56posuffadh5a367n5vi4uq 70 23 30 30 CD work_sny56posuffadh5a367n5vi4uq 70 24 s s NNP work_sny56posuffadh5a367n5vi4uq 70 25 , , , work_sny56posuffadh5a367n5vi4uq 70 26 67 67 CD work_sny56posuffadh5a367n5vi4uq 70 27 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 70 28 C C NNP work_sny56posuffadh5a367n5vi4uq 70 29 30 30 CD work_sny56posuffadh5a367n5vi4uq 70 30 s s NNP work_sny56posuffadh5a367n5vi4uq 70 31 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 70 32 , , , work_sny56posuffadh5a367n5vi4uq 70 33 25 25 CD work_sny56posuffadh5a367n5vi4uq 70 34 cycles cycle NNS work_sny56posuffadh5a367n5vi4uq 70 35 of of IN work_sny56posuffadh5a367n5vi4uq 70 36 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 70 37 94 94 CD work_sny56posuffadh5a367n5vi4uq 70 38 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 70 39 C C NNP work_sny56posuffadh5a367n5vi4uq 70 40 for for IN work_sny56posuffadh5a367n5vi4uq 70 41 30 30 CD work_sny56posuffadh5a367n5vi4uq 70 42 s s NN work_sny56posuffadh5a367n5vi4uq 70 43 , , , work_sny56posuffadh5a367n5vi4uq 70 44 67 67 CD work_sny56posuffadh5a367n5vi4uq 70 45 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 70 46 C C NNP work_sny56posuffadh5a367n5vi4uq 70 47 for for IN work_sny56posuffadh5a367n5vi4uq 70 48 30 30 CD work_sny56posuffadh5a367n5vi4uq 70 49 s s NN work_sny56posuffadh5a367n5vi4uq 70 50 , , , work_sny56posuffadh5a367n5vi4uq 70 51 72 72 CD work_sny56posuffadh5a367n5vi4uq 70 52 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 70 53 C C NNP work_sny56posuffadh5a367n5vi4uq 70 54 for for IN work_sny56posuffadh5a367n5vi4uq 70 55 5 5 CD work_sny56posuffadh5a367n5vi4uq 70 56 s s POS work_sny56posuffadh5a367n5vi4uq 70 57 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 70 58 followed follow VBN work_sny56posuffadh5a367n5vi4uq 70 59 by by IN work_sny56posuffadh5a367n5vi4uq 70 60 72 72 CD work_sny56posuffadh5a367n5vi4uq 70 61 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 70 62 C C NNP work_sny56posuffadh5a367n5vi4uq 70 63 for for IN work_sny56posuffadh5a367n5vi4uq 70 64 10 10 CD work_sny56posuffadh5a367n5vi4uq 70 65 min min NN work_sny56posuffadh5a367n5vi4uq 70 66 . . . work_sny56posuffadh5a367n5vi4uq 71 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 71 2 in in IN work_sny56posuffadh5a367n5vi4uq 71 3 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 71 4 liver liver NN work_sny56posuffadh5a367n5vi4uq 71 5 : : : work_sny56posuffadh5a367n5vi4uq 71 6 DNA DNA NNP work_sny56posuffadh5a367n5vi4uq 71 7 was be VBD work_sny56posuffadh5a367n5vi4uq 71 8 isolated isolate VBN work_sny56posuffadh5a367n5vi4uq 71 9 from from IN work_sny56posuffadh5a367n5vi4uq 71 10 8 8 CD work_sny56posuffadh5a367n5vi4uq 71 11 mm3 mm3 NNS work_sny56posuffadh5a367n5vi4uq 71 12 frozen frozen JJ work_sny56posuffadh5a367n5vi4uq 71 13 liver liver NN work_sny56posuffadh5a367n5vi4uq 71 14 specimens specimen NNS work_sny56posuffadh5a367n5vi4uq 71 15 using use VBG work_sny56posuffadh5a367n5vi4uq 71 16 the the DT work_sny56posuffadh5a367n5vi4uq 71 17 Puregene Puregene NNP work_sny56posuffadh5a367n5vi4uq 71 18 DNA DNA NNP work_sny56posuffadh5a367n5vi4uq 71 19 purification purification NN work_sny56posuffadh5a367n5vi4uq 71 20 kit kit NN work_sny56posuffadh5a367n5vi4uq 71 21 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 71 22 Gentra Gentra NNP work_sny56posuffadh5a367n5vi4uq 71 23 Systems Systems NNP work_sny56posuffadh5a367n5vi4uq 71 24 , , , work_sny56posuffadh5a367n5vi4uq 71 25 Minneapolis Minneapolis NNP work_sny56posuffadh5a367n5vi4uq 71 26 , , , work_sny56posuffadh5a367n5vi4uq 71 27 MN MN NNP work_sny56posuffadh5a367n5vi4uq 71 28 , , , work_sny56posuffadh5a367n5vi4uq 71 29 USA USA NNP work_sny56posuffadh5a367n5vi4uq 71 30 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 71 31 . . . work_sny56posuffadh5a367n5vi4uq 72 1 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 72 2 was be VBD work_sny56posuffadh5a367n5vi4uq 72 3 performed perform VBN work_sny56posuffadh5a367n5vi4uq 72 4 in in IN work_sny56posuffadh5a367n5vi4uq 72 5 two two CD work_sny56posuffadh5a367n5vi4uq 72 6 rounds round NNS work_sny56posuffadh5a367n5vi4uq 72 7 of of IN work_sny56posuffadh5a367n5vi4uq 72 8 amplification amplification NN work_sny56posuffadh5a367n5vi4uq 72 9 . . . work_sny56posuffadh5a367n5vi4uq 73 1 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 73 2 I -PRON- PRP work_sny56posuffadh5a367n5vi4uq 73 3 was be VBD work_sny56posuffadh5a367n5vi4uq 73 4 carried carry VBN work_sny56posuffadh5a367n5vi4uq 73 5 out out RP work_sny56posuffadh5a367n5vi4uq 73 6 in in IN work_sny56posuffadh5a367n5vi4uq 73 7 a a DT work_sny56posuffadh5a367n5vi4uq 73 8 50-µl 50-µl JJ work_sny56posuffadh5a367n5vi4uq 73 9 reaction reaction NN work_sny56posuffadh5a367n5vi4uq 73 10 mixture mixture NN work_sny56posuffadh5a367n5vi4uq 73 11 containing contain VBG work_sny56posuffadh5a367n5vi4uq 73 12 1 1 CD work_sny56posuffadh5a367n5vi4uq 73 13 µg µg IN work_sny56posuffadh5a367n5vi4uq 73 14 DNA dna NN work_sny56posuffadh5a367n5vi4uq 73 15 , , , work_sny56posuffadh5a367n5vi4uq 73 16 5 5 CD work_sny56posuffadh5a367n5vi4uq 73 17 % % NN work_sny56posuffadh5a367n5vi4uq 73 18 DMSO DMSO NNS work_sny56posuffadh5a367n5vi4uq 73 19 , , , work_sny56posuffadh5a367n5vi4uq 73 20 5 5 CD work_sny56posuffadh5a367n5vi4uq 73 21 % % NN work_sny56posuffadh5a367n5vi4uq 73 22 glycerol glycerol NN work_sny56posuffadh5a367n5vi4uq 73 23 , , , work_sny56posuffadh5a367n5vi4uq 73 24 10 10 CD work_sny56posuffadh5a367n5vi4uq 73 25 pmol pmol NN work_sny56posuffadh5a367n5vi4uq 73 26 each each DT work_sny56posuffadh5a367n5vi4uq 73 27 forward forward RB work_sny56posuffadh5a367n5vi4uq 73 28 and and CC work_sny56posuffadh5a367n5vi4uq 73 29 reverse reverse VB work_sny56posuffadh5a367n5vi4uq 73 30 primer primer NNP work_sny56posuffadh5a367n5vi4uq 73 31 , , , work_sny56posuffadh5a367n5vi4uq 73 32 a a DT work_sny56posuffadh5a367n5vi4uq 73 33 0.2 0.2 CD work_sny56posuffadh5a367n5vi4uq 73 34 mM mm NN work_sny56posuffadh5a367n5vi4uq 73 35 concentration concentration NN work_sny56posuffadh5a367n5vi4uq 73 36 of of IN work_sny56posuffadh5a367n5vi4uq 73 37 each each DT work_sny56posuffadh5a367n5vi4uq 73 38 dNTP dntp NN work_sny56posuffadh5a367n5vi4uq 73 39 , , , work_sny56posuffadh5a367n5vi4uq 73 40 1× 1× NNP work_sny56posuffadh5a367n5vi4uq 73 41 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 73 42 buffer buffer VBP work_sny56posuffadh5a367n5vi4uq 73 43 A a DT work_sny56posuffadh5a367n5vi4uq 73 44 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 73 45 Invitrogen Invitrogen NNP work_sny56posuffadh5a367n5vi4uq 73 46 , , , work_sny56posuffadh5a367n5vi4uq 73 47 Carlsbad Carlsbad NNP work_sny56posuffadh5a367n5vi4uq 73 48 , , , work_sny56posuffadh5a367n5vi4uq 73 49 WI WI NNP work_sny56posuffadh5a367n5vi4uq 73 50 , , , work_sny56posuffadh5a367n5vi4uq 73 51 USA USA NNP work_sny56posuffadh5a367n5vi4uq 73 52 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 73 53 , , , work_sny56posuffadh5a367n5vi4uq 73 54 and and CC work_sny56posuffadh5a367n5vi4uq 73 55 5U 5U NNP work_sny56posuffadh5a367n5vi4uq 73 56 Taq Taq NNP work_sny56posuffadh5a367n5vi4uq 73 57 DNA dna NN work_sny56posuffadh5a367n5vi4uq 73 58 polymerase polymerase NN work_sny56posuffadh5a367n5vi4uq 73 59 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 73 60 Promega Promega NNP work_sny56posuffadh5a367n5vi4uq 73 61 , , , work_sny56posuffadh5a367n5vi4uq 73 62 Madison Madison NNP work_sny56posuffadh5a367n5vi4uq 73 63 , , , work_sny56posuffadh5a367n5vi4uq 73 64 WI WI NNP work_sny56posuffadh5a367n5vi4uq 73 65 , , , work_sny56posuffadh5a367n5vi4uq 73 66 USA USA NNP work_sny56posuffadh5a367n5vi4uq 73 67 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 73 68 . . . work_sny56posuffadh5a367n5vi4uq 74 1 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 74 2 conditions condition NNS work_sny56posuffadh5a367n5vi4uq 74 3 were be VBD work_sny56posuffadh5a367n5vi4uq 74 4 : : : work_sny56posuffadh5a367n5vi4uq 74 5 95 95 CD work_sny56posuffadh5a367n5vi4uq 74 6 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 74 7 C C NNP work_sny56posuffadh5a367n5vi4uq 74 8 for for IN work_sny56posuffadh5a367n5vi4uq 74 9 5 5 CD work_sny56posuffadh5a367n5vi4uq 74 10 min min NN work_sny56posuffadh5a367n5vi4uq 74 11 followed follow VBN work_sny56posuffadh5a367n5vi4uq 74 12 by by IN work_sny56posuffadh5a367n5vi4uq 74 13 45 45 CD work_sny56posuffadh5a367n5vi4uq 74 14 cycles cycle NNS work_sny56posuffadh5a367n5vi4uq 74 15 of of IN work_sny56posuffadh5a367n5vi4uq 74 16 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 74 17 95 95 CD work_sny56posuffadh5a367n5vi4uq 74 18 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 74 19 C C NNP work_sny56posuffadh5a367n5vi4uq 74 20 , , , work_sny56posuffadh5a367n5vi4uq 74 21 40 40 CD work_sny56posuffadh5a367n5vi4uq 74 22 s s NN work_sny56posuffadh5a367n5vi4uq 74 23 , , , work_sny56posuffadh5a367n5vi4uq 74 24 58 58 CD work_sny56posuffadh5a367n5vi4uq 74 25 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 74 26 C C NNP work_sny56posuffadh5a367n5vi4uq 74 27 , , , work_sny56posuffadh5a367n5vi4uq 74 28 30 30 CD work_sny56posuffadh5a367n5vi4uq 74 29 s s NN work_sny56posuffadh5a367n5vi4uq 74 30 , , , work_sny56posuffadh5a367n5vi4uq 74 31 72 72 CD work_sny56posuffadh5a367n5vi4uq 74 32 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 74 33 C C NNP work_sny56posuffadh5a367n5vi4uq 74 34 for for IN work_sny56posuffadh5a367n5vi4uq 74 35 1 1 CD work_sny56posuffadh5a367n5vi4uq 74 36 min min NN work_sny56posuffadh5a367n5vi4uq 74 37 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 74 38 with with IN work_sny56posuffadh5a367n5vi4uq 74 39 the the DT work_sny56posuffadh5a367n5vi4uq 74 40 final final JJ work_sny56posuffadh5a367n5vi4uq 74 41 extension extension NN work_sny56posuffadh5a367n5vi4uq 74 42 of of IN work_sny56posuffadh5a367n5vi4uq 74 43 5 5 CD work_sny56posuffadh5a367n5vi4uq 74 44 min min NN work_sny56posuffadh5a367n5vi4uq 74 45 at at IN work_sny56posuffadh5a367n5vi4uq 74 46 72 72 CD work_sny56posuffadh5a367n5vi4uq 74 47 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 74 48 C. C. NNP work_sny56posuffadh5a367n5vi4uq 75 1 A a DT work_sny56posuffadh5a367n5vi4uq 75 2 10-µl 10-µl CD work_sny56posuffadh5a367n5vi4uq 75 3 aliquot aliquot NNS work_sny56posuffadh5a367n5vi4uq 75 4 of of IN work_sny56posuffadh5a367n5vi4uq 75 5 the the DT work_sny56posuffadh5a367n5vi4uq 75 6 primary primary JJ work_sny56posuffadh5a367n5vi4uq 75 7 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 75 8 product product NN work_sny56posuffadh5a367n5vi4uq 75 9 was be VBD work_sny56posuffadh5a367n5vi4uq 75 10 used use VBN work_sny56posuffadh5a367n5vi4uq 75 11 for for IN work_sny56posuffadh5a367n5vi4uq 75 12 secondary secondary JJ work_sny56posuffadh5a367n5vi4uq 75 13 amplification amplification NN work_sny56posuffadh5a367n5vi4uq 75 14 in in IN work_sny56posuffadh5a367n5vi4uq 75 15 a a DT work_sny56posuffadh5a367n5vi4uq 75 16 100-µl 100-µl CD work_sny56posuffadh5a367n5vi4uq 75 17 reaction reaction NN work_sny56posuffadh5a367n5vi4uq 75 18 with with IN work_sny56posuffadh5a367n5vi4uq 75 19 nested nested JJ work_sny56posuffadh5a367n5vi4uq 75 20 primers primer NNS work_sny56posuffadh5a367n5vi4uq 75 21 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 75 22 10 10 CD work_sny56posuffadh5a367n5vi4uq 75 23 µm µm NNP work_sny56posuffadh5a367n5vi4uq 75 24 concentration concentration NN work_sny56posuffadh5a367n5vi4uq 75 25 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 75 26 and and CC work_sny56posuffadh5a367n5vi4uq 75 27 the the DT work_sny56posuffadh5a367n5vi4uq 75 28 same same JJ work_sny56posuffadh5a367n5vi4uq 75 29 cycling cycling NN work_sny56posuffadh5a367n5vi4uq 75 30 conditions condition NNS work_sny56posuffadh5a367n5vi4uq 75 31 except except IN work_sny56posuffadh5a367n5vi4uq 75 32 that that IN work_sny56posuffadh5a367n5vi4uq 75 33 the the DT work_sny56posuffadh5a367n5vi4uq 75 34 number number NN work_sny56posuffadh5a367n5vi4uq 75 35 of of IN work_sny56posuffadh5a367n5vi4uq 75 36 cycles cycle NNS work_sny56posuffadh5a367n5vi4uq 75 37 was be VBD work_sny56posuffadh5a367n5vi4uq 75 38 35 35 CD work_sny56posuffadh5a367n5vi4uq 75 39 . . . work_sny56posuffadh5a367n5vi4uq 76 1 The the DT work_sny56posuffadh5a367n5vi4uq 76 2 expected expected JJ work_sny56posuffadh5a367n5vi4uq 76 3 size size NN work_sny56posuffadh5a367n5vi4uq 76 4 of of IN work_sny56posuffadh5a367n5vi4uq 76 5 the the DT work_sny56posuffadh5a367n5vi4uq 76 6 amplified amplified JJ work_sny56posuffadh5a367n5vi4uq 76 7 excision excision NN work_sny56posuffadh5a367n5vi4uq 76 8 product product NN work_sny56posuffadh5a367n5vi4uq 76 9 was be VBD work_sny56posuffadh5a367n5vi4uq 76 10 approximately approximately RB work_sny56posuffadh5a367n5vi4uq 76 11 456 456 CD work_sny56posuffadh5a367n5vi4uq 76 12 bp bp NN work_sny56posuffadh5a367n5vi4uq 76 13 . . . work_sny56posuffadh5a367n5vi4uq 77 1 The the DT work_sny56posuffadh5a367n5vi4uq 77 2 primers primer NNS work_sny56posuffadh5a367n5vi4uq 77 3 used use VBN work_sny56posuffadh5a367n5vi4uq 77 4 for for IN work_sny56posuffadh5a367n5vi4uq 77 5 the the DT work_sny56posuffadh5a367n5vi4uq 77 6 excision excision NN work_sny56posuffadh5a367n5vi4uq 77 7 assay assay NN work_sny56posuffadh5a367n5vi4uq 77 8 were be VBD work_sny56posuffadh5a367n5vi4uq 77 9 outside outside RB work_sny56posuffadh5a367n5vi4uq 77 10 left left JJ work_sny56posuffadh5a367n5vi4uq 77 11 and and CC work_sny56posuffadh5a367n5vi4uq 77 12 right right JJ work_sny56posuffadh5a367n5vi4uq 77 13 ITRs itr NNS work_sny56posuffadh5a367n5vi4uq 77 14 . . . work_sny56posuffadh5a367n5vi4uq 78 1 The the DT work_sny56posuffadh5a367n5vi4uq 78 2 primer primer JJ work_sny56posuffadh5a367n5vi4uq 78 3 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 78 4 were be VBD work_sny56posuffadh5a367n5vi4uq 78 5 : : : work_sny56posuffadh5a367n5vi4uq 78 6 FP1 FP1 NNP work_sny56posuffadh5a367n5vi4uq 78 7 : : : work_sny56posuffadh5a367n5vi4uq 78 8 5′-TGACGTTGGAGTCCACGTTC-3′ 5′-tgacgttggagtccacgttc-3′ CD work_sny56posuffadh5a367n5vi4uq 78 9 RP1 rp1 NN work_sny56posuffadh5a367n5vi4uq 78 10 : : : work_sny56posuffadh5a367n5vi4uq 78 11 5′-GGCTCGTATGTTGTGTGG-3′ 5′-ggctcgtatgttgtgtgg-3′ CD work_sny56posuffadh5a367n5vi4uq 78 12 FP2 fp2 NN work_sny56posuffadh5a367n5vi4uq 78 13 : : : work_sny56posuffadh5a367n5vi4uq 78 14 5′-CTGGAACAACACTCAACCCT-3′ 5′-ctggaacaacactcaaccct-3′ CD work_sny56posuffadh5a367n5vi4uq 78 15 RP2 RP2 NNS work_sny56posuffadh5a367n5vi4uq 78 16 : : : work_sny56posuffadh5a367n5vi4uq 78 17 5′-CACACAGGAAACAGCTATGA-3′. 5′-cacacaggaaacagctatga-3′. LS work_sny56posuffadh5a367n5vi4uq 79 1 Detection detection NN work_sny56posuffadh5a367n5vi4uq 79 2 and and CC work_sny56posuffadh5a367n5vi4uq 79 3 quantification quantification NN work_sny56posuffadh5a367n5vi4uq 79 4 of of IN work_sny56posuffadh5a367n5vi4uq 79 5 the the DT work_sny56posuffadh5a367n5vi4uq 79 6 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 79 7 product product NN work_sny56posuffadh5a367n5vi4uq 79 8 The the DT work_sny56posuffadh5a367n5vi4uq 79 9 excision excision NN work_sny56posuffadh5a367n5vi4uq 79 10 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 79 11 products product NNS work_sny56posuffadh5a367n5vi4uq 79 12 were be VBD work_sny56posuffadh5a367n5vi4uq 79 13 separated separate VBN work_sny56posuffadh5a367n5vi4uq 79 14 on on IN work_sny56posuffadh5a367n5vi4uq 79 15 3 3 CD work_sny56posuffadh5a367n5vi4uq 79 16 % % NN work_sny56posuffadh5a367n5vi4uq 79 17 low- low- NN work_sny56posuffadh5a367n5vi4uq 79 18 melt melt NN work_sny56posuffadh5a367n5vi4uq 79 19 gels gel NNS work_sny56posuffadh5a367n5vi4uq 79 20 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 79 21 GenePure GenePure NNP work_sny56posuffadh5a367n5vi4uq 79 22 Sieve Sieve NNP work_sny56posuffadh5a367n5vi4uq 79 23 GQA GQA NNP work_sny56posuffadh5a367n5vi4uq 79 24 agarose agarose NN work_sny56posuffadh5a367n5vi4uq 79 25 , , , work_sny56posuffadh5a367n5vi4uq 79 26 ISC ISC NNP work_sny56posuffadh5a367n5vi4uq 79 27 Bioexpress Bioexpress NNP work_sny56posuffadh5a367n5vi4uq 79 28 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 79 29 and and CC work_sny56posuffadh5a367n5vi4uq 79 30 stained stain VBN work_sny56posuffadh5a367n5vi4uq 79 31 with with IN work_sny56posuffadh5a367n5vi4uq 79 32 50 50 CD work_sny56posuffadh5a367n5vi4uq 79 33 µg µg CD work_sny56posuffadh5a367n5vi4uq 79 34 / / SYM work_sny56posuffadh5a367n5vi4uq 79 35 ml ml NNP work_sny56posuffadh5a367n5vi4uq 79 36 ethidium ethidium NN work_sny56posuffadh5a367n5vi4uq 79 37 bromide bromide NN work_sny56posuffadh5a367n5vi4uq 79 38 . . . work_sny56posuffadh5a367n5vi4uq 80 1 For for IN work_sny56posuffadh5a367n5vi4uq 80 2 better well JJR work_sny56posuffadh5a367n5vi4uq 80 3 resolution resolution NN work_sny56posuffadh5a367n5vi4uq 80 4 and and CC work_sny56posuffadh5a367n5vi4uq 80 5 quantification quantification NN work_sny56posuffadh5a367n5vi4uq 80 6 , , , work_sny56posuffadh5a367n5vi4uq 80 7 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 80 8 products product NNS work_sny56posuffadh5a367n5vi4uq 80 9 were be VBD work_sny56posuffadh5a367n5vi4uq 80 10 separated separate VBN work_sny56posuffadh5a367n5vi4uq 80 11 on on IN work_sny56posuffadh5a367n5vi4uq 80 12 6 6 CD work_sny56posuffadh5a367n5vi4uq 80 13 % % NN work_sny56posuffadh5a367n5vi4uq 80 14 polyacrylamide polyacrylamide NN work_sny56posuffadh5a367n5vi4uq 80 15 gels gel NNS work_sny56posuffadh5a367n5vi4uq 80 16 and and CC work_sny56posuffadh5a367n5vi4uq 80 17 stained stain VBD work_sny56posuffadh5a367n5vi4uq 80 18 with with IN work_sny56posuffadh5a367n5vi4uq 80 19 1 1 CD work_sny56posuffadh5a367n5vi4uq 80 20 : : SYM work_sny56posuffadh5a367n5vi4uq 80 21 10 10 CD work_sny56posuffadh5a367n5vi4uq 80 22 000 000 CD work_sny56posuffadh5a367n5vi4uq 80 23 dilution dilution NN work_sny56posuffadh5a367n5vi4uq 80 24 of of IN work_sny56posuffadh5a367n5vi4uq 80 25 SYBR SYBR NNP work_sny56posuffadh5a367n5vi4uq 80 26 green green JJ work_sny56posuffadh5a367n5vi4uq 80 27 I -PRON- PRP work_sny56posuffadh5a367n5vi4uq 80 28 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 80 29 Molecular Molecular NNP work_sny56posuffadh5a367n5vi4uq 80 30 Probes Probes NNPS work_sny56posuffadh5a367n5vi4uq 80 31 , , , work_sny56posuffadh5a367n5vi4uq 80 32 Inc. Inc. NNP work_sny56posuffadh5a367n5vi4uq 80 33 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 80 34 for for IN work_sny56posuffadh5a367n5vi4uq 80 35 45 45 CD work_sny56posuffadh5a367n5vi4uq 80 36 min min NN work_sny56posuffadh5a367n5vi4uq 80 37 . . . work_sny56posuffadh5a367n5vi4uq 81 1 The the DT work_sny56posuffadh5a367n5vi4uq 81 2 gels gel NNS work_sny56posuffadh5a367n5vi4uq 81 3 were be VBD work_sny56posuffadh5a367n5vi4uq 81 4 scanned scan VBN work_sny56posuffadh5a367n5vi4uq 81 5 with with IN work_sny56posuffadh5a367n5vi4uq 81 6 a a DT work_sny56posuffadh5a367n5vi4uq 81 7 Storm storm NN work_sny56posuffadh5a367n5vi4uq 81 8 phosphor phosphor NN work_sny56posuffadh5a367n5vi4uq 81 9 imager imager NN work_sny56posuffadh5a367n5vi4uq 81 10 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 81 11 Molecular Molecular NNP work_sny56posuffadh5a367n5vi4uq 81 12 Dynamics Dynamics NNPS work_sny56posuffadh5a367n5vi4uq 81 13 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 81 14 at at IN work_sny56posuffadh5a367n5vi4uq 81 15 850 850 CD work_sny56posuffadh5a367n5vi4uq 81 16 V v NN work_sny56posuffadh5a367n5vi4uq 81 17 in in IN work_sny56posuffadh5a367n5vi4uq 81 18 the the DT work_sny56posuffadh5a367n5vi4uq 81 19 blue blue JJ work_sny56posuffadh5a367n5vi4uq 81 20 fluorescence fluorescence NN work_sny56posuffadh5a367n5vi4uq 81 21 mode mode NN work_sny56posuffadh5a367n5vi4uq 81 22 to to TO work_sny56posuffadh5a367n5vi4uq 81 23 visualize visualize VB work_sny56posuffadh5a367n5vi4uq 81 24 the the DT work_sny56posuffadh5a367n5vi4uq 81 25 bands band NNS work_sny56posuffadh5a367n5vi4uq 81 26 . . . work_sny56posuffadh5a367n5vi4uq 82 1 To to TO work_sny56posuffadh5a367n5vi4uq 82 2 standardize standardize VB work_sny56posuffadh5a367n5vi4uq 82 3 the the DT work_sny56posuffadh5a367n5vi4uq 82 4 input input NN work_sny56posuffadh5a367n5vi4uq 82 5 total total JJ work_sny56posuffadh5a367n5vi4uq 82 6 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 82 7 DNA DNA NNP work_sny56posuffadh5a367n5vi4uq 82 8 for for IN work_sny56posuffadh5a367n5vi4uq 82 9 each each DT work_sny56posuffadh5a367n5vi4uq 82 10 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 82 11 , , , work_sny56posuffadh5a367n5vi4uq 82 12 a a DT work_sny56posuffadh5a367n5vi4uq 82 13 1/5000 1/5000 CD work_sny56posuffadh5a367n5vi4uq 82 14 dilution dilution NN work_sny56posuffadh5a367n5vi4uq 82 15 of of IN work_sny56posuffadh5a367n5vi4uq 82 16 initial initial JJ work_sny56posuffadh5a367n5vi4uq 82 17 lysate lysate NN work_sny56posuffadh5a367n5vi4uq 82 18 was be VBD work_sny56posuffadh5a367n5vi4uq 82 19 used use VBN work_sny56posuffadh5a367n5vi4uq 82 20 to to TO work_sny56posuffadh5a367n5vi4uq 82 21 amplify amplify VB work_sny56posuffadh5a367n5vi4uq 82 22 a a DT work_sny56posuffadh5a367n5vi4uq 82 23 segment segment NN work_sny56posuffadh5a367n5vi4uq 82 24 of of IN work_sny56posuffadh5a367n5vi4uq 82 25 the the DT work_sny56posuffadh5a367n5vi4uq 82 26 ampicillin ampicillin NNP work_sny56posuffadh5a367n5vi4uq 82 27 - - HYPH work_sny56posuffadh5a367n5vi4uq 82 28 resistance resistance NN work_sny56posuffadh5a367n5vi4uq 82 29 gene gene NN work_sny56posuffadh5a367n5vi4uq 82 30 on on IN work_sny56posuffadh5a367n5vi4uq 82 31 both both DT work_sny56posuffadh5a367n5vi4uq 82 32 pCMVSB pcmvsb NN work_sny56posuffadh5a367n5vi4uq 82 33 and and CC work_sny56posuffadh5a367n5vi4uq 82 34 input input NN work_sny56posuffadh5a367n5vi4uq 82 35 pT pT NNP work_sny56posuffadh5a367n5vi4uq 82 36 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 82 37 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 82 38 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 82 39 depending depend VBG work_sny56posuffadh5a367n5vi4uq 82 40 on on IN work_sny56posuffadh5a367n5vi4uq 82 41 the the DT work_sny56posuffadh5a367n5vi4uq 82 42 experiment experiment NN work_sny56posuffadh5a367n5vi4uq 82 43 , , , work_sny56posuffadh5a367n5vi4uq 82 44 it -PRON- PRP work_sny56posuffadh5a367n5vi4uq 82 45 could could MD work_sny56posuffadh5a367n5vi4uq 82 46 be be VB work_sny56posuffadh5a367n5vi4uq 82 47 pT pT NNP work_sny56posuffadh5a367n5vi4uq 82 48 / / SYM work_sny56posuffadh5a367n5vi4uq 82 49 HindIIIneo hindiiineo NN work_sny56posuffadh5a367n5vi4uq 82 50 , , , work_sny56posuffadh5a367n5vi4uq 82 51 one one CD work_sny56posuffadh5a367n5vi4uq 82 52 of of IN work_sny56posuffadh5a367n5vi4uq 82 53 the the DT work_sny56posuffadh5a367n5vi4uq 82 54 mutants mutant NNS work_sny56posuffadh5a367n5vi4uq 82 55 , , , work_sny56posuffadh5a367n5vi4uq 82 56 or or CC work_sny56posuffadh5a367n5vi4uq 82 57 pT pT NNP work_sny56posuffadh5a367n5vi4uq 82 58 / / SYM work_sny56posuffadh5a367n5vi4uq 82 59 neo neo NN work_sny56posuffadh5a367n5vi4uq 82 60 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 82 61 . . . work_sny56posuffadh5a367n5vi4uq 83 1 These these DT work_sny56posuffadh5a367n5vi4uq 83 2 products product NNS work_sny56posuffadh5a367n5vi4uq 83 3 were be VBD work_sny56posuffadh5a367n5vi4uq 83 4 separated separate VBN work_sny56posuffadh5a367n5vi4uq 83 5 on on IN work_sny56posuffadh5a367n5vi4uq 83 6 1 1 CD work_sny56posuffadh5a367n5vi4uq 83 7 % % NN work_sny56posuffadh5a367n5vi4uq 83 8 agarose agarose JJ work_sny56posuffadh5a367n5vi4uq 83 9 gel gel NN work_sny56posuffadh5a367n5vi4uq 83 10 and and CC work_sny56posuffadh5a367n5vi4uq 83 11 stained stain VBN work_sny56posuffadh5a367n5vi4uq 83 12 with with IN work_sny56posuffadh5a367n5vi4uq 83 13 50 50 CD work_sny56posuffadh5a367n5vi4uq 83 14 µg µg CD work_sny56posuffadh5a367n5vi4uq 83 15 / / SYM work_sny56posuffadh5a367n5vi4uq 83 16 ml ml NNP work_sny56posuffadh5a367n5vi4uq 83 17 ethidium ethidium NN work_sny56posuffadh5a367n5vi4uq 83 18 bromide bromide NN work_sny56posuffadh5a367n5vi4uq 83 19 . . . work_sny56posuffadh5a367n5vi4uq 84 1 The the DT work_sny56posuffadh5a367n5vi4uq 84 2 intensity intensity NN work_sny56posuffadh5a367n5vi4uq 84 3 of of IN work_sny56posuffadh5a367n5vi4uq 84 4 each each DT work_sny56posuffadh5a367n5vi4uq 84 5 band band NN work_sny56posuffadh5a367n5vi4uq 84 6 was be VBD work_sny56posuffadh5a367n5vi4uq 84 7 measured measure VBN work_sny56posuffadh5a367n5vi4uq 84 8 in in IN work_sny56posuffadh5a367n5vi4uq 84 9 arbitrary arbitrary JJ work_sny56posuffadh5a367n5vi4uq 84 10 increments increment NNS work_sny56posuffadh5a367n5vi4uq 84 11 of of IN work_sny56posuffadh5a367n5vi4uq 84 12 density density NN work_sny56posuffadh5a367n5vi4uq 84 13 units unit NNS work_sny56posuffadh5a367n5vi4uq 84 14 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 84 15 integrated integrate VBN work_sny56posuffadh5a367n5vi4uq 84 16 optical optical JJ work_sny56posuffadh5a367n5vi4uq 84 17 density density NN work_sny56posuffadh5a367n5vi4uq 84 18 , , , work_sny56posuffadh5a367n5vi4uq 84 19 IOD IOD NNP work_sny56posuffadh5a367n5vi4uq 84 20 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 84 21 using use VBG work_sny56posuffadh5a367n5vi4uq 84 22 Gel Gel NNP work_sny56posuffadh5a367n5vi4uq 84 23 - - HYPH work_sny56posuffadh5a367n5vi4uq 84 24 Pro Pro NNP work_sny56posuffadh5a367n5vi4uq 84 25 analyzer analyzer NN work_sny56posuffadh5a367n5vi4uq 84 26 imaging image VBG work_sny56posuffadh5a367n5vi4uq 84 27 software software NN work_sny56posuffadh5a367n5vi4uq 84 28 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 84 29 Media Media NNP work_sny56posuffadh5a367n5vi4uq 84 30 Cybernetics Cybernetics NNP work_sny56posuffadh5a367n5vi4uq 84 31 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 84 32 . . . work_sny56posuffadh5a367n5vi4uq 85 1 The the DT work_sny56posuffadh5a367n5vi4uq 85 2 background background NN work_sny56posuffadh5a367n5vi4uq 85 3 was be VBD work_sny56posuffadh5a367n5vi4uq 85 4 corrected correct VBN work_sny56posuffadh5a367n5vi4uq 85 5 using use VBG work_sny56posuffadh5a367n5vi4uq 85 6 the the DT work_sny56posuffadh5a367n5vi4uq 85 7 filtered filtered JJ work_sny56posuffadh5a367n5vi4uq 85 8 profile profile NN work_sny56posuffadh5a367n5vi4uq 85 9 method method NN work_sny56posuffadh5a367n5vi4uq 85 10 , , , work_sny56posuffadh5a367n5vi4uq 85 11 as as IN work_sny56posuffadh5a367n5vi4uq 85 12 instructed instruct VBN work_sny56posuffadh5a367n5vi4uq 85 13 in in IN work_sny56posuffadh5a367n5vi4uq 85 14 the the DT work_sny56posuffadh5a367n5vi4uq 85 15 manual manual NN work_sny56posuffadh5a367n5vi4uq 85 16 . . . work_sny56posuffadh5a367n5vi4uq 86 1 The the DT work_sny56posuffadh5a367n5vi4uq 86 2 relative relative JJ work_sny56posuffadh5a367n5vi4uq 86 3 excision excision NN work_sny56posuffadh5a367n5vi4uq 86 4 activity activity NN work_sny56posuffadh5a367n5vi4uq 86 5 of of IN work_sny56posuffadh5a367n5vi4uq 86 6 the the DT work_sny56posuffadh5a367n5vi4uq 86 7 mutated mutate VBN work_sny56posuffadh5a367n5vi4uq 86 8 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 86 9 constructs construct NNS work_sny56posuffadh5a367n5vi4uq 86 10 was be VBD work_sny56posuffadh5a367n5vi4uq 86 11 derived derive VBN work_sny56posuffadh5a367n5vi4uq 86 12 from from IN work_sny56posuffadh5a367n5vi4uq 86 13 their -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 86 14 standardized standardized JJ work_sny56posuffadh5a367n5vi4uq 86 15 IOD IOD NNP work_sny56posuffadh5a367n5vi4uq 86 16 , , , work_sny56posuffadh5a367n5vi4uq 86 17 i.e. i.e. FW work_sny56posuffadh5a367n5vi4uq 86 18 , , , work_sny56posuffadh5a367n5vi4uq 86 19 the the DT work_sny56posuffadh5a367n5vi4uq 86 20 IOD IOD NNP work_sny56posuffadh5a367n5vi4uq 86 21 of of IN work_sny56posuffadh5a367n5vi4uq 86 22 the the DT work_sny56posuffadh5a367n5vi4uq 86 23 excision excision NN work_sny56posuffadh5a367n5vi4uq 86 24 band band NN work_sny56posuffadh5a367n5vi4uq 86 25 divided divide VBN work_sny56posuffadh5a367n5vi4uq 86 26 by by IN work_sny56posuffadh5a367n5vi4uq 86 27 the the DT work_sny56posuffadh5a367n5vi4uq 86 28 relative relative JJ work_sny56posuffadh5a367n5vi4uq 86 29 input input NN work_sny56posuffadh5a367n5vi4uq 86 30 template template NN work_sny56posuffadh5a367n5vi4uq 86 31 . . . work_sny56posuffadh5a367n5vi4uq 87 1 The the DT work_sny56posuffadh5a367n5vi4uq 87 2 relative relative JJ work_sny56posuffadh5a367n5vi4uq 87 3 excision excision NN work_sny56posuffadh5a367n5vi4uq 87 4 activity activity NN work_sny56posuffadh5a367n5vi4uq 87 5 is be VBZ work_sny56posuffadh5a367n5vi4uq 87 6 indicated indicate VBN work_sny56posuffadh5a367n5vi4uq 87 7 as as IN work_sny56posuffadh5a367n5vi4uq 87 8 a a DT work_sny56posuffadh5a367n5vi4uq 87 9 percentage percentage NN work_sny56posuffadh5a367n5vi4uq 87 10 of of IN work_sny56posuffadh5a367n5vi4uq 87 11 the the DT work_sny56posuffadh5a367n5vi4uq 87 12 excision excision NN work_sny56posuffadh5a367n5vi4uq 87 13 activity activity NN work_sny56posuffadh5a367n5vi4uq 87 14 of of IN work_sny56posuffadh5a367n5vi4uq 87 15 the the DT work_sny56posuffadh5a367n5vi4uq 87 16 control control NN work_sny56posuffadh5a367n5vi4uq 87 17 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 87 18 pT pT NNP work_sny56posuffadh5a367n5vi4uq 87 19 / / SYM work_sny56posuffadh5a367n5vi4uq 87 20 HindIIIneo HindIIIneo NNP work_sny56posuffadh5a367n5vi4uq 87 21 , , , work_sny56posuffadh5a367n5vi4uq 87 22 for for IN work_sny56posuffadh5a367n5vi4uq 87 23 which which WDT work_sny56posuffadh5a367n5vi4uq 87 24 a a DT work_sny56posuffadh5a367n5vi4uq 87 25 standard standard JJ work_sny56posuffadh5a367n5vi4uq 87 26 curve curve NN work_sny56posuffadh5a367n5vi4uq 87 27 Copyright copyright NN work_sny56posuffadh5a367n5vi4uq 87 28   CD work_sny56posuffadh5a367n5vi4uq 87 29 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 87 30 John John NNP work_sny56posuffadh5a367n5vi4uq 87 31 Wiley Wiley NNP work_sny56posuffadh5a367n5vi4uq 87 32 & & CC work_sny56posuffadh5a367n5vi4uq 87 33 Sons Sons NNPS work_sny56posuffadh5a367n5vi4uq 87 34 , , , work_sny56posuffadh5a367n5vi4uq 87 35 Ltd. Ltd. NNP work_sny56posuffadh5a367n5vi4uq 87 36 J J NNP work_sny56posuffadh5a367n5vi4uq 87 37 Gene Gene NNP work_sny56posuffadh5a367n5vi4uq 87 38 Med Med NNP work_sny56posuffadh5a367n5vi4uq 87 39 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 87 40 ; ; : work_sny56posuffadh5a367n5vi4uq 87 41 6 6 CD work_sny56posuffadh5a367n5vi4uq 87 42 : : : work_sny56posuffadh5a367n5vi4uq 87 43 574 574 CD work_sny56posuffadh5a367n5vi4uq 87 44 – – : work_sny56posuffadh5a367n5vi4uq 87 45 583 583 CD work_sny56posuffadh5a367n5vi4uq 87 46 . . . work_sny56posuffadh5a367n5vi4uq 88 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 88 2 of of IN work_sny56posuffadh5a367n5vi4uq 88 3 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 88 4 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 88 5 Transposons Transposons NNP work_sny56posuffadh5a367n5vi4uq 88 6 577 577 CD work_sny56posuffadh5a367n5vi4uq 88 7 derived derive VBN work_sny56posuffadh5a367n5vi4uq 88 8 from from IN work_sny56posuffadh5a367n5vi4uq 88 9 the the DT work_sny56posuffadh5a367n5vi4uq 88 10 ratio ratio NN work_sny56posuffadh5a367n5vi4uq 88 11 of of IN work_sny56posuffadh5a367n5vi4uq 88 12 the the DT work_sny56posuffadh5a367n5vi4uq 88 13 different different JJ work_sny56posuffadh5a367n5vi4uq 88 14 dilutions dilution NNS work_sny56posuffadh5a367n5vi4uq 88 15 of of IN work_sny56posuffadh5a367n5vi4uq 88 16 the the DT work_sny56posuffadh5a367n5vi4uq 88 17 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 88 18 was be VBD work_sny56posuffadh5a367n5vi4uq 88 19 constructed construct VBN work_sny56posuffadh5a367n5vi4uq 88 20 . . . work_sny56posuffadh5a367n5vi4uq 89 1 Footprint footprint NN work_sny56posuffadh5a367n5vi4uq 89 2 sequencing sequence VBG work_sny56posuffadh5a367n5vi4uq 89 3 To to TO work_sny56posuffadh5a367n5vi4uq 89 4 sequence sequence VB work_sny56posuffadh5a367n5vi4uq 89 5 the the DT work_sny56posuffadh5a367n5vi4uq 89 6 footprint footprint NN work_sny56posuffadh5a367n5vi4uq 89 7 , , , work_sny56posuffadh5a367n5vi4uq 89 8 the the DT work_sny56posuffadh5a367n5vi4uq 89 9 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 89 10 products product NNS work_sny56posuffadh5a367n5vi4uq 89 11 were be VBD work_sny56posuffadh5a367n5vi4uq 89 12 gel- gel- NNP work_sny56posuffadh5a367n5vi4uq 89 13 extracted extract VBN work_sny56posuffadh5a367n5vi4uq 89 14 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 89 15 for for IN work_sny56posuffadh5a367n5vi4uq 89 16 mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 89 17 with with IN work_sny56posuffadh5a367n5vi4uq 89 18 low low JJ work_sny56posuffadh5a367n5vi4uq 89 19 excision excision NN work_sny56posuffadh5a367n5vi4uq 89 20 activity activity NN work_sny56posuffadh5a367n5vi4uq 89 21 , , , work_sny56posuffadh5a367n5vi4uq 89 22 reamplified reamplifie VBN work_sny56posuffadh5a367n5vi4uq 89 23 with with IN work_sny56posuffadh5a367n5vi4uq 89 24 the the DT work_sny56posuffadh5a367n5vi4uq 89 25 nested nest VBN work_sny56posuffadh5a367n5vi4uq 89 26 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 89 27 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 89 28 , , , work_sny56posuffadh5a367n5vi4uq 89 29 cloned clone VBN work_sny56posuffadh5a367n5vi4uq 89 30 into into IN work_sny56posuffadh5a367n5vi4uq 89 31 the the DT work_sny56posuffadh5a367n5vi4uq 89 32 TOPO topo NN work_sny56posuffadh5a367n5vi4uq 89 33 TA TA NNP work_sny56posuffadh5a367n5vi4uq 89 34 cloning clone VBG work_sny56posuffadh5a367n5vi4uq 89 35 vector vector NN work_sny56posuffadh5a367n5vi4uq 89 36 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 89 37 Invitrogen Invitrogen NNP work_sny56posuffadh5a367n5vi4uq 89 38 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 89 39 and and CC work_sny56posuffadh5a367n5vi4uq 89 40 sequenced sequence VBN work_sny56posuffadh5a367n5vi4uq 89 41 by by IN work_sny56posuffadh5a367n5vi4uq 89 42 the the DT work_sny56posuffadh5a367n5vi4uq 89 43 Advanced Advanced NNP work_sny56posuffadh5a367n5vi4uq 89 44 Genetics Genetics NNP work_sny56posuffadh5a367n5vi4uq 89 45 Analysis Analysis NNP work_sny56posuffadh5a367n5vi4uq 89 46 Center Center NNP work_sny56posuffadh5a367n5vi4uq 89 47 at at IN work_sny56posuffadh5a367n5vi4uq 89 48 the the DT work_sny56posuffadh5a367n5vi4uq 89 49 University University NNP work_sny56posuffadh5a367n5vi4uq 89 50 of of IN work_sny56posuffadh5a367n5vi4uq 89 51 Minnesota Minnesota NNP work_sny56posuffadh5a367n5vi4uq 89 52 . . . work_sny56posuffadh5a367n5vi4uq 90 1 Transposon transposon JJ work_sny56posuffadh5a367n5vi4uq 90 2 delivery delivery NN work_sny56posuffadh5a367n5vi4uq 90 3 to to TO work_sny56posuffadh5a367n5vi4uq 90 4 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 90 5 liver liver NN work_sny56posuffadh5a367n5vi4uq 90 6 The the DT work_sny56posuffadh5a367n5vi4uq 90 7 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 90 8 - - HYPH work_sny56posuffadh5a367n5vi4uq 90 9 containing contain VBG work_sny56posuffadh5a367n5vi4uq 90 10 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 90 11 pT pT NNP work_sny56posuffadh5a367n5vi4uq 90 12 / / SYM work_sny56posuffadh5a367n5vi4uq 90 13 CAGGS CAGGS NNP work_sny56posuffadh5a367n5vi4uq 90 14 - - HYPH work_sny56posuffadh5a367n5vi4uq 90 15 GUSB GUSB NNP work_sny56posuffadh5a367n5vi4uq 90 16 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 90 17 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 90 18 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 90 19 and and CC work_sny56posuffadh5a367n5vi4uq 90 20 the the DT work_sny56posuffadh5a367n5vi4uq 90 21 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 90 22 - - HYPH work_sny56posuffadh5a367n5vi4uq 90 23 expressing express VBG work_sny56posuffadh5a367n5vi4uq 90 24 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 90 25 pCMVSB pcmvsb NN work_sny56posuffadh5a367n5vi4uq 90 26 were be VBD work_sny56posuffadh5a367n5vi4uq 90 27 used use VBN work_sny56posuffadh5a367n5vi4uq 90 28 to to TO work_sny56posuffadh5a367n5vi4uq 90 29 assay assay VB work_sny56posuffadh5a367n5vi4uq 90 30 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 90 31 in in IN work_sny56posuffadh5a367n5vi4uq 90 32 adult adult NN work_sny56posuffadh5a367n5vi4uq 90 33 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 90 34 tissues tissue NNS work_sny56posuffadh5a367n5vi4uq 90 35 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 90 36 Aronovich Aronovich NNP work_sny56posuffadh5a367n5vi4uq 90 37 et et FW work_sny56posuffadh5a367n5vi4uq 90 38 al al NNP work_sny56posuffadh5a367n5vi4uq 90 39 . . NNP work_sny56posuffadh5a367n5vi4uq 90 40 , , , work_sny56posuffadh5a367n5vi4uq 90 41 manuscript manuscript NNP work_sny56posuffadh5a367n5vi4uq 90 42 in in IN work_sny56posuffadh5a367n5vi4uq 90 43 prep prep NNP work_sny56posuffadh5a367n5vi4uq 90 44 . . . work_sny56posuffadh5a367n5vi4uq 90 45 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 90 46 . . . work_sny56posuffadh5a367n5vi4uq 91 1 The the DT work_sny56posuffadh5a367n5vi4uq 91 2 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 91 3 contains contain VBZ work_sny56posuffadh5a367n5vi4uq 91 4 an an DT work_sny56posuffadh5a367n5vi4uq 91 5 expression expression NN work_sny56posuffadh5a367n5vi4uq 91 6 cassette cassette NN work_sny56posuffadh5a367n5vi4uq 91 7 for for IN work_sny56posuffadh5a367n5vi4uq 91 8 the the DT work_sny56posuffadh5a367n5vi4uq 91 9 GUSB GUSB NNP work_sny56posuffadh5a367n5vi4uq 91 10 gene gene NN work_sny56posuffadh5a367n5vi4uq 91 11 to to TO work_sny56posuffadh5a367n5vi4uq 91 12 restore restore VB work_sny56posuffadh5a367n5vi4uq 91 13 activity activity NN work_sny56posuffadh5a367n5vi4uq 91 14 to to IN work_sny56posuffadh5a367n5vi4uq 91 15 mutant mutant JJ work_sny56posuffadh5a367n5vi4uq 91 16 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 91 17 deficient deficient JJ work_sny56posuffadh5a367n5vi4uq 91 18 in in IN work_sny56posuffadh5a367n5vi4uq 91 19 β- β- NNP work_sny56posuffadh5a367n5vi4uq 91 20 glucuronidase glucuronidase NNP work_sny56posuffadh5a367n5vi4uq 91 21 activity activity NN work_sny56posuffadh5a367n5vi4uq 91 22 . . . work_sny56posuffadh5a367n5vi4uq 92 1 The the DT work_sny56posuffadh5a367n5vi4uq 92 2 mucopolysaccharidosis mucopolysaccharidosis NN work_sny56posuffadh5a367n5vi4uq 92 3 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 92 4 MPS MPS NNP work_sny56posuffadh5a367n5vi4uq 92 5 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 92 6 type type NN work_sny56posuffadh5a367n5vi4uq 92 7 VII vii NN work_sny56posuffadh5a367n5vi4uq 92 8 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 92 9 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 92 10 B6.C B6.C NNP work_sny56posuffadh5a367n5vi4uq 92 11 - - HYPH work_sny56posuffadh5a367n5vi4uq 92 12 H-2bml H-2bml NNP work_sny56posuffadh5a367n5vi4uq 92 13 / / SYM work_sny56posuffadh5a367n5vi4uq 92 14 ByBir ByBir NNP work_sny56posuffadh5a367n5vi4uq 92 15 - - HYPH work_sny56posuffadh5a367n5vi4uq 92 16 gusmps gusmps NNP work_sny56posuffadh5a367n5vi4uq 92 17 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 92 18 were be VBD work_sny56posuffadh5a367n5vi4uq 92 19 obtained obtain VBN work_sny56posuffadh5a367n5vi4uq 92 20 from from IN work_sny56posuffadh5a367n5vi4uq 92 21 Jackson Jackson NNP work_sny56posuffadh5a367n5vi4uq 92 22 Laboratories Laboratories NNP work_sny56posuffadh5a367n5vi4uq 92 23 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 92 24 Bar Bar NNP work_sny56posuffadh5a367n5vi4uq 92 25 Harbor Harbor NNP work_sny56posuffadh5a367n5vi4uq 92 26 , , , work_sny56posuffadh5a367n5vi4uq 92 27 ME ME NNP work_sny56posuffadh5a367n5vi4uq 92 28 , , , work_sny56posuffadh5a367n5vi4uq 92 29 USA USA NNP work_sny56posuffadh5a367n5vi4uq 92 30 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 92 31 and and CC work_sny56posuffadh5a367n5vi4uq 92 32 maintained maintain VBD work_sny56posuffadh5a367n5vi4uq 92 33 in in IN work_sny56posuffadh5a367n5vi4uq 92 34 the the DT work_sny56posuffadh5a367n5vi4uq 92 35 AAALAC AAALAC NNP work_sny56posuffadh5a367n5vi4uq 92 36 - - HYPH work_sny56posuffadh5a367n5vi4uq 92 37 accredited accredit VBN work_sny56posuffadh5a367n5vi4uq 92 38 specific specific JJ work_sny56posuffadh5a367n5vi4uq 92 39 pathogen- pathogen- NNP work_sny56posuffadh5a367n5vi4uq 92 40 free free JJ work_sny56posuffadh5a367n5vi4uq 92 41 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 92 42 facility facility NN work_sny56posuffadh5a367n5vi4uq 92 43 at at IN work_sny56posuffadh5a367n5vi4uq 92 44 the the DT work_sny56posuffadh5a367n5vi4uq 92 45 University University NNP work_sny56posuffadh5a367n5vi4uq 92 46 of of IN work_sny56posuffadh5a367n5vi4uq 92 47 Minnesota Minnesota NNP work_sny56posuffadh5a367n5vi4uq 92 48 . . . work_sny56posuffadh5a367n5vi4uq 93 1 The the DT work_sny56posuffadh5a367n5vi4uq 93 2 plasmids plasmid NNS work_sny56posuffadh5a367n5vi4uq 93 3 were be VBD work_sny56posuffadh5a367n5vi4uq 93 4 injected inject VBN work_sny56posuffadh5a367n5vi4uq 93 5 into into IN work_sny56posuffadh5a367n5vi4uq 93 6 the the DT work_sny56posuffadh5a367n5vi4uq 93 7 tail tail NN work_sny56posuffadh5a367n5vi4uq 93 8 vein vein NN work_sny56posuffadh5a367n5vi4uq 93 9 of of IN work_sny56posuffadh5a367n5vi4uq 93 10 homozygous homozygous JJ work_sny56posuffadh5a367n5vi4uq 93 11 12 12 CD work_sny56posuffadh5a367n5vi4uq 93 12 – – : work_sny56posuffadh5a367n5vi4uq 93 13 16-week 16-week CD work_sny56posuffadh5a367n5vi4uq 93 14 - - HYPH work_sny56posuffadh5a367n5vi4uq 93 15 old old JJ work_sny56posuffadh5a367n5vi4uq 93 16 MPS MPS NNP work_sny56posuffadh5a367n5vi4uq 93 17 VII VII NNP work_sny56posuffadh5a367n5vi4uq 93 18 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 93 19 using use VBG work_sny56posuffadh5a367n5vi4uq 93 20 a a DT work_sny56posuffadh5a367n5vi4uq 93 21 3-ml 3-ml CD work_sny56posuffadh5a367n5vi4uq 93 22 latex latex NN work_sny56posuffadh5a367n5vi4uq 93 23 - - HYPH work_sny56posuffadh5a367n5vi4uq 93 24 free free JJ work_sny56posuffadh5a367n5vi4uq 93 25 syringe syringe NN work_sny56posuffadh5a367n5vi4uq 93 26 with with IN work_sny56posuffadh5a367n5vi4uq 93 27 a a DT work_sny56posuffadh5a367n5vi4uq 93 28 271/2 271/2 NNP work_sny56posuffadh5a367n5vi4uq 93 29 G g NN work_sny56posuffadh5a367n5vi4uq 93 30 needle needle NN work_sny56posuffadh5a367n5vi4uq 93 31 . . . work_sny56posuffadh5a367n5vi4uq 94 1 The the DT work_sny56posuffadh5a367n5vi4uq 94 2 hydrodynamics hydrodynamic NNS work_sny56posuffadh5a367n5vi4uq 94 3 - - HYPH work_sny56posuffadh5a367n5vi4uq 94 4 based base VBN work_sny56posuffadh5a367n5vi4uq 94 5 procedure procedure NN work_sny56posuffadh5a367n5vi4uq 94 6 was be VBD work_sny56posuffadh5a367n5vi4uq 94 7 performed perform VBN work_sny56posuffadh5a367n5vi4uq 94 8 as as IN work_sny56posuffadh5a367n5vi4uq 94 9 described describe VBN work_sny56posuffadh5a367n5vi4uq 94 10 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 94 11 21,22 21,22 CD work_sny56posuffadh5a367n5vi4uq 94 12 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 94 13 . . . work_sny56posuffadh5a367n5vi4uq 95 1 Each each DT work_sny56posuffadh5a367n5vi4uq 95 2 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 95 3 received receive VBD work_sny56posuffadh5a367n5vi4uq 95 4 plasmid plasmid JJ work_sny56posuffadh5a367n5vi4uq 95 5 DNA dna NN work_sny56posuffadh5a367n5vi4uq 95 6 in in IN work_sny56posuffadh5a367n5vi4uq 95 7 lactated lactate VBN work_sny56posuffadh5a367n5vi4uq 95 8 Ringer Ringer NNP work_sny56posuffadh5a367n5vi4uq 95 9 ’s ’s POS work_sny56posuffadh5a367n5vi4uq 95 10 solution solution NN work_sny56posuffadh5a367n5vi4uq 95 11 in in IN work_sny56posuffadh5a367n5vi4uq 95 12 a a DT work_sny56posuffadh5a367n5vi4uq 95 13 total total JJ work_sny56posuffadh5a367n5vi4uq 95 14 volume volume NN work_sny56posuffadh5a367n5vi4uq 95 15 equal equal JJ work_sny56posuffadh5a367n5vi4uq 95 16 to to IN work_sny56posuffadh5a367n5vi4uq 95 17 10 10 CD work_sny56posuffadh5a367n5vi4uq 95 18 % % NN work_sny56posuffadh5a367n5vi4uq 95 19 of of IN work_sny56posuffadh5a367n5vi4uq 95 20 body body NN work_sny56posuffadh5a367n5vi4uq 95 21 weight weight NN work_sny56posuffadh5a367n5vi4uq 95 22 . . . work_sny56posuffadh5a367n5vi4uq 96 1 25 25 CD work_sny56posuffadh5a367n5vi4uq 96 2 µg µg IN work_sny56posuffadh5a367n5vi4uq 96 3 of of IN work_sny56posuffadh5a367n5vi4uq 96 4 a a DT work_sny56posuffadh5a367n5vi4uq 96 5 single single JJ work_sny56posuffadh5a367n5vi4uq 96 6 preparation preparation NN work_sny56posuffadh5a367n5vi4uq 96 7 of of IN work_sny56posuffadh5a367n5vi4uq 96 8 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 96 9 pT pT NNP work_sny56posuffadh5a367n5vi4uq 96 10 / / SYM work_sny56posuffadh5a367n5vi4uq 96 11 CAGGS CAGGS NNP work_sny56posuffadh5a367n5vi4uq 96 12 - - HYPH work_sny56posuffadh5a367n5vi4uq 96 13 GUSB GUSB NNP work_sny56posuffadh5a367n5vi4uq 96 14 were be VBD work_sny56posuffadh5a367n5vi4uq 96 15 injected inject VBN work_sny56posuffadh5a367n5vi4uq 96 16 either either CC work_sny56posuffadh5a367n5vi4uq 96 17 alone alone RB work_sny56posuffadh5a367n5vi4uq 96 18 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 96 19 ‘ ' `` work_sny56posuffadh5a367n5vi4uq 96 20 Treatment Treatment NNP work_sny56posuffadh5a367n5vi4uq 96 21 Group Group NNP work_sny56posuffadh5a367n5vi4uq 96 22 1 1 CD work_sny56posuffadh5a367n5vi4uq 96 23 ’ ' '' work_sny56posuffadh5a367n5vi4uq 96 24 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 96 25 , , , work_sny56posuffadh5a367n5vi4uq 96 26 or or CC work_sny56posuffadh5a367n5vi4uq 96 27 with with IN work_sny56posuffadh5a367n5vi4uq 96 28 pCMVSB pcmvsb NN work_sny56posuffadh5a367n5vi4uq 96 29 at at IN work_sny56posuffadh5a367n5vi4uq 96 30 10 10 CD work_sny56posuffadh5a367n5vi4uq 96 31 : : SYM work_sny56posuffadh5a367n5vi4uq 96 32 1 1 CD work_sny56posuffadh5a367n5vi4uq 96 33 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 96 34 / / SYM work_sny56posuffadh5a367n5vi4uq 96 35 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 96 36 molar molar JJ work_sny56posuffadh5a367n5vi4uq 96 37 ratio ratio NN work_sny56posuffadh5a367n5vi4uq 96 38 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 96 39 ‘ ' `` work_sny56posuffadh5a367n5vi4uq 96 40 Treatment Treatment NNP work_sny56posuffadh5a367n5vi4uq 96 41 Group Group NNP work_sny56posuffadh5a367n5vi4uq 96 42 2 2 CD work_sny56posuffadh5a367n5vi4uq 96 43 ’ ' '' work_sny56posuffadh5a367n5vi4uq 96 44 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 96 45 . . . work_sny56posuffadh5a367n5vi4uq 97 1 37.5 37.5 CD work_sny56posuffadh5a367n5vi4uq 97 2 µg µg IN work_sny56posuffadh5a367n5vi4uq 97 3 DNA DNA NNP work_sny56posuffadh5a367n5vi4uq 97 4 were be VBD work_sny56posuffadh5a367n5vi4uq 97 5 injected inject VBN work_sny56posuffadh5a367n5vi4uq 97 6 into into IN work_sny56posuffadh5a367n5vi4uq 97 7 all all DT work_sny56posuffadh5a367n5vi4uq 97 8 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 97 9 with with IN work_sny56posuffadh5a367n5vi4uq 97 10 pBluescript pBluescript NNP work_sny56posuffadh5a367n5vi4uq 97 11 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 97 12 as as IN work_sny56posuffadh5a367n5vi4uq 97 13 a a DT work_sny56posuffadh5a367n5vi4uq 97 14 ‘ ' `` work_sny56posuffadh5a367n5vi4uq 97 15 filler filler NN work_sny56posuffadh5a367n5vi4uq 97 16 ’ ' '' work_sny56posuffadh5a367n5vi4uq 97 17 ; ; : work_sny56posuffadh5a367n5vi4uq 97 18 the the DT work_sny56posuffadh5a367n5vi4uq 97 19 sham sham NNP work_sny56posuffadh5a367n5vi4uq 97 20 treatment treatment NNP work_sny56posuffadh5a367n5vi4uq 97 21 control control NNP work_sny56posuffadh5a367n5vi4uq 97 22 group group NN work_sny56posuffadh5a367n5vi4uq 97 23 of of IN work_sny56posuffadh5a367n5vi4uq 97 24 MPS MPS NNP work_sny56posuffadh5a367n5vi4uq 97 25 VII VII NNP work_sny56posuffadh5a367n5vi4uq 97 26 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 97 27 was be VBD work_sny56posuffadh5a367n5vi4uq 97 28 injected inject VBN work_sny56posuffadh5a367n5vi4uq 97 29 with with IN work_sny56posuffadh5a367n5vi4uq 97 30 pBluescript pBluescript NNP work_sny56posuffadh5a367n5vi4uq 97 31 alone alone RB work_sny56posuffadh5a367n5vi4uq 97 32 . . . work_sny56posuffadh5a367n5vi4uq 98 1 All all DT work_sny56posuffadh5a367n5vi4uq 98 2 injections injection NNS work_sny56posuffadh5a367n5vi4uq 98 3 were be VBD work_sny56posuffadh5a367n5vi4uq 98 4 performed perform VBN work_sny56posuffadh5a367n5vi4uq 98 5 only only RB work_sny56posuffadh5a367n5vi4uq 98 6 once once RB work_sny56posuffadh5a367n5vi4uq 98 7 . . . work_sny56posuffadh5a367n5vi4uq 99 1 The the DT work_sny56posuffadh5a367n5vi4uq 99 2 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 99 3 were be VBD work_sny56posuffadh5a367n5vi4uq 99 4 euthanized euthanize VBN work_sny56posuffadh5a367n5vi4uq 99 5 1-week 1-week CD work_sny56posuffadh5a367n5vi4uq 99 6 post post NN work_sny56posuffadh5a367n5vi4uq 99 7 - - NN work_sny56posuffadh5a367n5vi4uq 99 8 injection injection NN work_sny56posuffadh5a367n5vi4uq 99 9 , , , work_sny56posuffadh5a367n5vi4uq 99 10 livers liver NNS work_sny56posuffadh5a367n5vi4uq 99 11 were be VBD work_sny56posuffadh5a367n5vi4uq 99 12 harvested harvest VBN work_sny56posuffadh5a367n5vi4uq 99 13 and and CC work_sny56posuffadh5a367n5vi4uq 99 14 frozen freeze VBN work_sny56posuffadh5a367n5vi4uq 99 15 at at IN work_sny56posuffadh5a367n5vi4uq 99 16 −80 −80 NNP work_sny56posuffadh5a367n5vi4uq 99 17 ◦ ◦ NNP work_sny56posuffadh5a367n5vi4uq 99 18 C. C. NNP work_sny56posuffadh5a367n5vi4uq 100 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 100 2 assays assay NNS work_sny56posuffadh5a367n5vi4uq 100 3 were be VBD work_sny56posuffadh5a367n5vi4uq 100 4 performed perform VBN work_sny56posuffadh5a367n5vi4uq 100 5 as as IN work_sny56posuffadh5a367n5vi4uq 100 6 described describe VBN work_sny56posuffadh5a367n5vi4uq 100 7 above above RB work_sny56posuffadh5a367n5vi4uq 100 8 . . . work_sny56posuffadh5a367n5vi4uq 101 1 Results result NNS work_sny56posuffadh5a367n5vi4uq 101 2 Assay Assay NNP work_sny56posuffadh5a367n5vi4uq 101 3 for for IN work_sny56posuffadh5a367n5vi4uq 101 4 the the DT work_sny56posuffadh5a367n5vi4uq 101 5 excision excision NN work_sny56posuffadh5a367n5vi4uq 101 6 step step NN work_sny56posuffadh5a367n5vi4uq 101 7 of of IN work_sny56posuffadh5a367n5vi4uq 101 8 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 101 9 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 101 10 developed develop VBD work_sny56posuffadh5a367n5vi4uq 101 11 and and CC work_sny56posuffadh5a367n5vi4uq 101 12 used use VBD work_sny56posuffadh5a367n5vi4uq 101 13 the the DT work_sny56posuffadh5a367n5vi4uq 101 14 excision excision NN work_sny56posuffadh5a367n5vi4uq 101 15 assay assay NN work_sny56posuffadh5a367n5vi4uq 101 16 first first RB work_sny56posuffadh5a367n5vi4uq 101 17 in in IN work_sny56posuffadh5a367n5vi4uq 101 18 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 101 19 cells cell NNS work_sny56posuffadh5a367n5vi4uq 101 20 where where WRB work_sny56posuffadh5a367n5vi4uq 101 21 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 101 22 co co VBP work_sny56posuffadh5a367n5vi4uq 101 23 - - VBD work_sny56posuffadh5a367n5vi4uq 101 24 transfected transfecte VBD work_sny56posuffadh5a367n5vi4uq 101 25 the the DT work_sny56posuffadh5a367n5vi4uq 101 26 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 101 27 plasmid plasmid NNP work_sny56posuffadh5a367n5vi4uq 101 28 pT pT NNP work_sny56posuffadh5a367n5vi4uq 101 29 / / SYM work_sny56posuffadh5a367n5vi4uq 101 30 neo neo NNP work_sny56posuffadh5a367n5vi4uq 101 31 and and CC work_sny56posuffadh5a367n5vi4uq 101 32 the the DT work_sny56posuffadh5a367n5vi4uq 101 33 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 101 34 - - HYPH work_sny56posuffadh5a367n5vi4uq 101 35 expressing express VBG work_sny56posuffadh5a367n5vi4uq 101 36 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 101 37 pCMVSB pcmvsb NN work_sny56posuffadh5a367n5vi4uq 101 38 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 101 39 Figure figure NN work_sny56posuffadh5a367n5vi4uq 101 40 2 2 CD work_sny56posuffadh5a367n5vi4uq 101 41 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 101 42 . . . work_sny56posuffadh5a367n5vi4uq 102 1 Three three CD work_sny56posuffadh5a367n5vi4uq 102 2 days day NNS work_sny56posuffadh5a367n5vi4uq 102 3 after after IN work_sny56posuffadh5a367n5vi4uq 102 4 transfection transfection NN work_sny56posuffadh5a367n5vi4uq 102 5 , , , work_sny56posuffadh5a367n5vi4uq 102 6 the the DT work_sny56posuffadh5a367n5vi4uq 102 7 plasmids plasmid NNS work_sny56posuffadh5a367n5vi4uq 102 8 in in IN work_sny56posuffadh5a367n5vi4uq 102 9 the the DT work_sny56posuffadh5a367n5vi4uq 102 10 cell cell NN work_sny56posuffadh5a367n5vi4uq 102 11 lysate lysate NN work_sny56posuffadh5a367n5vi4uq 102 12 were be VBD work_sny56posuffadh5a367n5vi4uq 102 13 used use VBN work_sny56posuffadh5a367n5vi4uq 102 14 as as IN work_sny56posuffadh5a367n5vi4uq 102 15 templates template NNS work_sny56posuffadh5a367n5vi4uq 102 16 for for IN work_sny56posuffadh5a367n5vi4uq 102 17 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 102 18 . . . work_sny56posuffadh5a367n5vi4uq 103 1 Using use VBG work_sny56posuffadh5a367n5vi4uq 103 2 primers primer NNS work_sny56posuffadh5a367n5vi4uq 103 3 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 103 4 the the DT work_sny56posuffadh5a367n5vi4uq 103 5 donor donor NN work_sny56posuffadh5a367n5vi4uq 103 6 sites site NNS work_sny56posuffadh5a367n5vi4uq 103 7 , , , work_sny56posuffadh5a367n5vi4uq 103 8 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 103 9 detected detect VBD work_sny56posuffadh5a367n5vi4uq 103 10 excision excision NN work_sny56posuffadh5a367n5vi4uq 103 11 by by IN work_sny56posuffadh5a367n5vi4uq 103 12 amplifying amplify VBG work_sny56posuffadh5a367n5vi4uq 103 13 a a DT work_sny56posuffadh5a367n5vi4uq 103 14 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 103 15 product product NN work_sny56posuffadh5a367n5vi4uq 103 16 that that WDT work_sny56posuffadh5a367n5vi4uq 103 17 corresponded correspond VBD work_sny56posuffadh5a367n5vi4uq 103 18 to to IN work_sny56posuffadh5a367n5vi4uq 103 19 the the DT work_sny56posuffadh5a367n5vi4uq 103 20 size size NN work_sny56posuffadh5a367n5vi4uq 103 21 of of IN work_sny56posuffadh5a367n5vi4uq 103 22 a a DT work_sny56posuffadh5a367n5vi4uq 103 23 rejoined rejoin VBN work_sny56posuffadh5a367n5vi4uq 103 24 vector vector NN work_sny56posuffadh5a367n5vi4uq 103 25 after after IN work_sny56posuffadh5a367n5vi4uq 103 26 the the DT work_sny56posuffadh5a367n5vi4uq 103 27 excision excision NN work_sny56posuffadh5a367n5vi4uq 103 28 of of IN work_sny56posuffadh5a367n5vi4uq 103 29 the the DT work_sny56posuffadh5a367n5vi4uq 103 30 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 103 31 . . . work_sny56posuffadh5a367n5vi4uq 104 1 As as IN work_sny56posuffadh5a367n5vi4uq 104 2 shown show VBN work_sny56posuffadh5a367n5vi4uq 104 3 in in IN work_sny56posuffadh5a367n5vi4uq 104 4 Figure Figure NNP work_sny56posuffadh5a367n5vi4uq 104 5 3A 3A NNP work_sny56posuffadh5a367n5vi4uq 104 6 , , , work_sny56posuffadh5a367n5vi4uq 104 7 a a DT work_sny56posuffadh5a367n5vi4uq 104 8 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 104 9 product product NN work_sny56posuffadh5a367n5vi4uq 104 10 of of IN work_sny56posuffadh5a367n5vi4uq 104 11 Figure figure NN work_sny56posuffadh5a367n5vi4uq 104 12 2 2 CD work_sny56posuffadh5a367n5vi4uq 104 13 . . . work_sny56posuffadh5a367n5vi4uq 105 1 Schematic schematic JJ work_sny56posuffadh5a367n5vi4uq 105 2 of of IN work_sny56posuffadh5a367n5vi4uq 105 3 the the DT work_sny56posuffadh5a367n5vi4uq 105 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 105 5 assay assay NN work_sny56posuffadh5a367n5vi4uq 105 6 . . . work_sny56posuffadh5a367n5vi4uq 106 1 Plasmids plasmid NNS work_sny56posuffadh5a367n5vi4uq 106 2 containing contain VBG work_sny56posuffadh5a367n5vi4uq 106 3 a a DT work_sny56posuffadh5a367n5vi4uq 106 4 transposon transposon NN work_sny56posuffadh5a367n5vi4uq 106 5 and and CC work_sny56posuffadh5a367n5vi4uq 106 6 CMVSB CMVSB NNP work_sny56posuffadh5a367n5vi4uq 106 7 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 106 8 were be VBD work_sny56posuffadh5a367n5vi4uq 106 9 co co VBN work_sny56posuffadh5a367n5vi4uq 106 10 - - VBN work_sny56posuffadh5a367n5vi4uq 106 11 transfected transfected JJ work_sny56posuffadh5a367n5vi4uq 106 12 into into IN work_sny56posuffadh5a367n5vi4uq 106 13 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 106 14 cells cell NNS work_sny56posuffadh5a367n5vi4uq 106 15 . . . work_sny56posuffadh5a367n5vi4uq 107 1 Four four CD work_sny56posuffadh5a367n5vi4uq 107 2 days day NNS work_sny56posuffadh5a367n5vi4uq 107 3 post post NN work_sny56posuffadh5a367n5vi4uq 107 4 - - JJ work_sny56posuffadh5a367n5vi4uq 107 5 transfection transfection NN work_sny56posuffadh5a367n5vi4uq 107 6 , , , work_sny56posuffadh5a367n5vi4uq 107 7 cell cell NN work_sny56posuffadh5a367n5vi4uq 107 8 lysates lysate NNS work_sny56posuffadh5a367n5vi4uq 107 9 were be VBD work_sny56posuffadh5a367n5vi4uq 107 10 obtained obtain VBN work_sny56posuffadh5a367n5vi4uq 107 11 and and CC work_sny56posuffadh5a367n5vi4uq 107 12 used use VBN work_sny56posuffadh5a367n5vi4uq 107 13 for for IN work_sny56posuffadh5a367n5vi4uq 107 14 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 107 15 with with IN work_sny56posuffadh5a367n5vi4uq 107 16 primers primer NNS work_sny56posuffadh5a367n5vi4uq 107 17 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 107 18 the the DT work_sny56posuffadh5a367n5vi4uq 107 19 donor donor NN work_sny56posuffadh5a367n5vi4uq 107 20 sites site NNS work_sny56posuffadh5a367n5vi4uq 107 21 . . . work_sny56posuffadh5a367n5vi4uq 108 1 The the DT work_sny56posuffadh5a367n5vi4uq 108 2 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 108 3 products product NNS work_sny56posuffadh5a367n5vi4uq 108 4 were be VBD work_sny56posuffadh5a367n5vi4uq 108 5 sequenced sequence VBN work_sny56posuffadh5a367n5vi4uq 108 6 to to TO work_sny56posuffadh5a367n5vi4uq 108 7 determine determine VB work_sny56posuffadh5a367n5vi4uq 108 8 the the DT work_sny56posuffadh5a367n5vi4uq 108 9 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 108 10 of of IN work_sny56posuffadh5a367n5vi4uq 108 11 the the DT work_sny56posuffadh5a367n5vi4uq 108 12 excision excision NN work_sny56posuffadh5a367n5vi4uq 108 13 . . . work_sny56posuffadh5a367n5vi4uq 109 1 The the DT work_sny56posuffadh5a367n5vi4uq 109 2 procedure procedure NN work_sny56posuffadh5a367n5vi4uq 109 3 is be VBZ work_sny56posuffadh5a367n5vi4uq 109 4 shown show VBN work_sny56posuffadh5a367n5vi4uq 109 5 on on IN work_sny56posuffadh5a367n5vi4uq 109 6 the the DT work_sny56posuffadh5a367n5vi4uq 109 7 left left NN work_sny56posuffadh5a367n5vi4uq 109 8 and and CC work_sny56posuffadh5a367n5vi4uq 109 9 the the DT work_sny56posuffadh5a367n5vi4uq 109 10 state state NN work_sny56posuffadh5a367n5vi4uq 109 11 of of IN work_sny56posuffadh5a367n5vi4uq 109 12 the the DT work_sny56posuffadh5a367n5vi4uq 109 13 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 109 14 and and CC work_sny56posuffadh5a367n5vi4uq 109 15 its -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 109 16 excision excision NN work_sny56posuffadh5a367n5vi4uq 109 17 product product NN work_sny56posuffadh5a367n5vi4uq 109 18 is be VBZ work_sny56posuffadh5a367n5vi4uq 109 19 shown show VBN work_sny56posuffadh5a367n5vi4uq 109 20 on on IN work_sny56posuffadh5a367n5vi4uq 109 21 the the DT work_sny56posuffadh5a367n5vi4uq 109 22 right right NN work_sny56posuffadh5a367n5vi4uq 109 23 approximately approximately RB work_sny56posuffadh5a367n5vi4uq 109 24 582 582 CD work_sny56posuffadh5a367n5vi4uq 109 25 bp bp NN work_sny56posuffadh5a367n5vi4uq 109 26 was be VBD work_sny56posuffadh5a367n5vi4uq 109 27 amplified amplify VBN work_sny56posuffadh5a367n5vi4uq 109 28 in in IN work_sny56posuffadh5a367n5vi4uq 109 29 cell cell NN work_sny56posuffadh5a367n5vi4uq 109 30 lysates lysate NNS work_sny56posuffadh5a367n5vi4uq 109 31 from from IN work_sny56posuffadh5a367n5vi4uq 109 32 a a DT work_sny56posuffadh5a367n5vi4uq 109 33 pCMVSB pcmvsb NN work_sny56posuffadh5a367n5vi4uq 109 34 and and CC work_sny56posuffadh5a367n5vi4uq 109 35 pT pT NNP work_sny56posuffadh5a367n5vi4uq 109 36 / / SYM work_sny56posuffadh5a367n5vi4uq 109 37 neo neo NNP work_sny56posuffadh5a367n5vi4uq 109 38 co co NN work_sny56posuffadh5a367n5vi4uq 109 39 - - NN work_sny56posuffadh5a367n5vi4uq 109 40 transfection transfection NN work_sny56posuffadh5a367n5vi4uq 109 41 . . . work_sny56posuffadh5a367n5vi4uq 110 1 The the DT work_sny56posuffadh5a367n5vi4uq 110 2 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 110 3 product product NN work_sny56posuffadh5a367n5vi4uq 110 4 appeared appear VBD work_sny56posuffadh5a367n5vi4uq 110 5 to to TO work_sny56posuffadh5a367n5vi4uq 110 6 be be VB work_sny56posuffadh5a367n5vi4uq 110 7 specific specific JJ work_sny56posuffadh5a367n5vi4uq 110 8 to to IN work_sny56posuffadh5a367n5vi4uq 110 9 SB SB NNP work_sny56posuffadh5a367n5vi4uq 110 10 excision excision NN work_sny56posuffadh5a367n5vi4uq 110 11 because because IN work_sny56posuffadh5a367n5vi4uq 110 12 neither neither DT work_sny56posuffadh5a367n5vi4uq 110 13 pSB- pSB- NNP work_sny56posuffadh5a367n5vi4uq 110 14 � � NNP work_sny56posuffadh5a367n5vi4uq 110 15 DDE DDE NNP work_sny56posuffadh5a367n5vi4uq 110 16 , , , work_sny56posuffadh5a367n5vi4uq 110 17 which which WDT work_sny56posuffadh5a367n5vi4uq 110 18 has have VBZ work_sny56posuffadh5a367n5vi4uq 110 19 a a DT work_sny56posuffadh5a367n5vi4uq 110 20 mutated mutated JJ work_sny56posuffadh5a367n5vi4uq 110 21 catalytic catalytic JJ work_sny56posuffadh5a367n5vi4uq 110 22 domain domain NN work_sny56posuffadh5a367n5vi4uq 110 23 , , , work_sny56posuffadh5a367n5vi4uq 110 24 the the DT work_sny56posuffadh5a367n5vi4uq 110 25 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 110 26 plasmids plasmid NNS work_sny56posuffadh5a367n5vi4uq 110 27 alone alone RB work_sny56posuffadh5a367n5vi4uq 110 28 , , , work_sny56posuffadh5a367n5vi4uq 110 29 nor nor CC work_sny56posuffadh5a367n5vi4uq 110 30 the the DT work_sny56posuffadh5a367n5vi4uq 110 31 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 110 32 plasmids plasmid NNS work_sny56posuffadh5a367n5vi4uq 110 33 alone alone RB work_sny56posuffadh5a367n5vi4uq 110 34 , , , work_sny56posuffadh5a367n5vi4uq 110 35 produced produce VBD work_sny56posuffadh5a367n5vi4uq 110 36 the the DT work_sny56posuffadh5a367n5vi4uq 110 37 582-bp 582-bp CD work_sny56posuffadh5a367n5vi4uq 110 38 product product NN work_sny56posuffadh5a367n5vi4uq 110 39 . . . work_sny56posuffadh5a367n5vi4uq 111 1 Figure figure NN work_sny56posuffadh5a367n5vi4uq 111 2 3B 3B NNP work_sny56posuffadh5a367n5vi4uq 111 3 shows show VBZ work_sny56posuffadh5a367n5vi4uq 111 4 the the DT work_sny56posuffadh5a367n5vi4uq 111 5 accumulation accumulation NN work_sny56posuffadh5a367n5vi4uq 111 6 of of IN work_sny56posuffadh5a367n5vi4uq 111 7 the the DT work_sny56posuffadh5a367n5vi4uq 111 8 excision excision NN work_sny56posuffadh5a367n5vi4uq 111 9 products product NNS work_sny56posuffadh5a367n5vi4uq 111 10 over over IN work_sny56posuffadh5a367n5vi4uq 111 11 several several JJ work_sny56posuffadh5a367n5vi4uq 111 12 days day NNS work_sny56posuffadh5a367n5vi4uq 111 13 . . . work_sny56posuffadh5a367n5vi4uq 112 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 112 2 products product NNS work_sny56posuffadh5a367n5vi4uq 112 3 were be VBD work_sny56posuffadh5a367n5vi4uq 112 4 detectable detectable JJ work_sny56posuffadh5a367n5vi4uq 112 5 18 18 CD work_sny56posuffadh5a367n5vi4uq 112 6 h h NN work_sny56posuffadh5a367n5vi4uq 112 7 after after IN work_sny56posuffadh5a367n5vi4uq 112 8 transfection transfection NN work_sny56posuffadh5a367n5vi4uq 112 9 and and CC work_sny56posuffadh5a367n5vi4uq 112 10 their -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 112 11 levels level NNS work_sny56posuffadh5a367n5vi4uq 112 12 continued continue VBD work_sny56posuffadh5a367n5vi4uq 112 13 to to TO work_sny56posuffadh5a367n5vi4uq 112 14 rise rise VB work_sny56posuffadh5a367n5vi4uq 112 15 through through IN work_sny56posuffadh5a367n5vi4uq 112 16 89 89 CD work_sny56posuffadh5a367n5vi4uq 112 17 h h NN work_sny56posuffadh5a367n5vi4uq 112 18 after after IN work_sny56posuffadh5a367n5vi4uq 112 19 transfection transfection NN work_sny56posuffadh5a367n5vi4uq 112 20 . . . work_sny56posuffadh5a367n5vi4uq 113 1 In in IN work_sny56posuffadh5a367n5vi4uq 113 2 subsequent subsequent JJ work_sny56posuffadh5a367n5vi4uq 113 3 experiments experiment NNS work_sny56posuffadh5a367n5vi4uq 113 4 , , , work_sny56posuffadh5a367n5vi4uq 113 5 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 113 6 collected collect VBD work_sny56posuffadh5a367n5vi4uq 113 7 samples sample NNS work_sny56posuffadh5a367n5vi4uq 113 8 at at IN work_sny56posuffadh5a367n5vi4uq 113 9 72 72 CD work_sny56posuffadh5a367n5vi4uq 113 10 h h NN work_sny56posuffadh5a367n5vi4uq 113 11 for for IN work_sny56posuffadh5a367n5vi4uq 113 12 quantification quantification NN work_sny56posuffadh5a367n5vi4uq 113 13 of of IN work_sny56posuffadh5a367n5vi4uq 113 14 excision excision NN work_sny56posuffadh5a367n5vi4uq 113 15 events event NNS work_sny56posuffadh5a367n5vi4uq 113 16 under under IN work_sny56posuffadh5a367n5vi4uq 113 17 different different JJ work_sny56posuffadh5a367n5vi4uq 113 18 conditions condition NNS work_sny56posuffadh5a367n5vi4uq 113 19 . . . work_sny56posuffadh5a367n5vi4uq 114 1 Figure figure NN work_sny56posuffadh5a367n5vi4uq 114 2 3C 3c NN work_sny56posuffadh5a367n5vi4uq 114 3 shows show VBZ work_sny56posuffadh5a367n5vi4uq 114 4 the the DT work_sny56posuffadh5a367n5vi4uq 114 5 same same JJ work_sny56posuffadh5a367n5vi4uq 114 6 excision excision NN work_sny56posuffadh5a367n5vi4uq 114 7 assay assay NN work_sny56posuffadh5a367n5vi4uq 114 8 carried carry VBD work_sny56posuffadh5a367n5vi4uq 114 9 out out RP work_sny56posuffadh5a367n5vi4uq 114 10 in in IN work_sny56posuffadh5a367n5vi4uq 114 11 zebrafish zebrafish JJ work_sny56posuffadh5a367n5vi4uq 114 12 embryos embryo NNS work_sny56posuffadh5a367n5vi4uq 114 13 . . . work_sny56posuffadh5a367n5vi4uq 115 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 115 2 co co VBD work_sny56posuffadh5a367n5vi4uq 115 3 - - VBD work_sny56posuffadh5a367n5vi4uq 115 4 injected inject VBD work_sny56posuffadh5a367n5vi4uq 115 5 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 115 6 plasmids plasmid NNS work_sny56posuffadh5a367n5vi4uq 115 7 and and CC work_sny56posuffadh5a367n5vi4uq 115 8 SB SB NNP work_sny56posuffadh5a367n5vi4uq 115 9 mRNA mrna NN work_sny56posuffadh5a367n5vi4uq 115 10 as as IN work_sny56posuffadh5a367n5vi4uq 115 11 the the DT work_sny56posuffadh5a367n5vi4uq 115 12 source source NN work_sny56posuffadh5a367n5vi4uq 115 13 of of IN work_sny56posuffadh5a367n5vi4uq 115 14 the the DT work_sny56posuffadh5a367n5vi4uq 115 15 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 115 16 . . . work_sny56posuffadh5a367n5vi4uq 116 1 Extracts extract NNS work_sny56posuffadh5a367n5vi4uq 116 2 of of IN work_sny56posuffadh5a367n5vi4uq 116 3 24-h 24-h CD work_sny56posuffadh5a367n5vi4uq 116 4 embryos embryo NNS work_sny56posuffadh5a367n5vi4uq 116 5 were be VBD work_sny56posuffadh5a367n5vi4uq 116 6 used use VBN work_sny56posuffadh5a367n5vi4uq 116 7 for for IN work_sny56posuffadh5a367n5vi4uq 116 8 analysis analysis NN work_sny56posuffadh5a367n5vi4uq 116 9 of of IN work_sny56posuffadh5a367n5vi4uq 116 10 excision excision NN work_sny56posuffadh5a367n5vi4uq 116 11 by by IN work_sny56posuffadh5a367n5vi4uq 116 12 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 116 13 . . . work_sny56posuffadh5a367n5vi4uq 117 1 As as IN work_sny56posuffadh5a367n5vi4uq 117 2 with with IN work_sny56posuffadh5a367n5vi4uq 117 3 the the DT work_sny56posuffadh5a367n5vi4uq 117 4 cell cell NN work_sny56posuffadh5a367n5vi4uq 117 5 cultures culture NNS work_sny56posuffadh5a367n5vi4uq 117 6 , , , work_sny56posuffadh5a367n5vi4uq 117 7 excision excision NN work_sny56posuffadh5a367n5vi4uq 117 8 was be VBD work_sny56posuffadh5a367n5vi4uq 117 9 evident evident JJ work_sny56posuffadh5a367n5vi4uq 117 10 only only RB work_sny56posuffadh5a367n5vi4uq 117 11 in in IN work_sny56posuffadh5a367n5vi4uq 117 12 the the DT work_sny56posuffadh5a367n5vi4uq 117 13 presence presence NN work_sny56posuffadh5a367n5vi4uq 117 14 of of IN work_sny56posuffadh5a367n5vi4uq 117 15 SB SB NNP work_sny56posuffadh5a367n5vi4uq 117 16 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 117 17 , , , work_sny56posuffadh5a367n5vi4uq 117 18 which which WDT work_sny56posuffadh5a367n5vi4uq 117 19 supports support VBZ work_sny56posuffadh5a367n5vi4uq 117 20 our -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 117 21 previous previous JJ work_sny56posuffadh5a367n5vi4uq 117 22 findings finding NNS work_sny56posuffadh5a367n5vi4uq 117 23 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 117 24 9 9 CD work_sny56posuffadh5a367n5vi4uq 117 25 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 117 26 . . . work_sny56posuffadh5a367n5vi4uq 118 1 Footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 118 2 of of IN work_sny56posuffadh5a367n5vi4uq 118 3 SB SB NNP work_sny56posuffadh5a367n5vi4uq 118 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 118 5 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 118 6 cloned clone VBD work_sny56posuffadh5a367n5vi4uq 118 7 and and CC work_sny56posuffadh5a367n5vi4uq 118 8 sequenced sequence VBD work_sny56posuffadh5a367n5vi4uq 118 9 the the DT work_sny56posuffadh5a367n5vi4uq 118 10 excision excision NN work_sny56posuffadh5a367n5vi4uq 118 11 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 118 12 products product NNS work_sny56posuffadh5a367n5vi4uq 118 13 to to TO work_sny56posuffadh5a367n5vi4uq 118 14 study study VB work_sny56posuffadh5a367n5vi4uq 118 15 the the DT work_sny56posuffadh5a367n5vi4uq 118 16 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 118 17 left leave VBN work_sny56posuffadh5a367n5vi4uq 118 18 by by IN work_sny56posuffadh5a367n5vi4uq 118 19 the the DT work_sny56posuffadh5a367n5vi4uq 118 20 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 118 21 . . . work_sny56posuffadh5a367n5vi4uq 119 1 Table table NN work_sny56posuffadh5a367n5vi4uq 119 2 1 1 CD work_sny56posuffadh5a367n5vi4uq 119 3 shows show VBZ work_sny56posuffadh5a367n5vi4uq 119 4 the the DT work_sny56posuffadh5a367n5vi4uq 119 5 summary summary NN work_sny56posuffadh5a367n5vi4uq 119 6 of of IN work_sny56posuffadh5a367n5vi4uq 119 7 the the DT work_sny56posuffadh5a367n5vi4uq 119 8 footprint footprint NN work_sny56posuffadh5a367n5vi4uq 119 9 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 119 10 acquired acquire VBD work_sny56posuffadh5a367n5vi4uq 119 11 from from IN work_sny56posuffadh5a367n5vi4uq 119 12 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 119 13 cells cell NNS work_sny56posuffadh5a367n5vi4uq 119 14 and and CC work_sny56posuffadh5a367n5vi4uq 119 15 zebrafish zebrafish JJ work_sny56posuffadh5a367n5vi4uq 119 16 embryos embryo NNS work_sny56posuffadh5a367n5vi4uq 119 17 . . . work_sny56posuffadh5a367n5vi4uq 120 1 The the DT work_sny56posuffadh5a367n5vi4uq 120 2 two two CD work_sny56posuffadh5a367n5vi4uq 120 3 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 120 4 sides side NNS work_sny56posuffadh5a367n5vi4uq 120 5 of of IN work_sny56posuffadh5a367n5vi4uq 120 6 the the DT work_sny56posuffadh5a367n5vi4uq 120 7 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 120 8 had have VBD work_sny56posuffadh5a367n5vi4uq 120 9 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 120 10 of of IN work_sny56posuffadh5a367n5vi4uq 120 11 varying vary VBG work_sny56posuffadh5a367n5vi4uq 120 12 lengths length NNS work_sny56posuffadh5a367n5vi4uq 120 13 that that WDT work_sny56posuffadh5a367n5vi4uq 120 14 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 120 15 categorized categorize VBD work_sny56posuffadh5a367n5vi4uq 120 16 in in IN work_sny56posuffadh5a367n5vi4uq 120 17 one one CD work_sny56posuffadh5a367n5vi4uq 120 18 of of IN work_sny56posuffadh5a367n5vi4uq 120 19 three three CD work_sny56posuffadh5a367n5vi4uq 120 20 ways way NNS work_sny56posuffadh5a367n5vi4uq 120 21 – – : work_sny56posuffadh5a367n5vi4uq 120 22 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 120 23 footprint footprint NN work_sny56posuffadh5a367n5vi4uq 120 24 , , , work_sny56posuffadh5a367n5vi4uq 120 25 non non JJ work_sny56posuffadh5a367n5vi4uq 120 26 - - JJ work_sny56posuffadh5a367n5vi4uq 120 27 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 120 28 footprint footprint NN work_sny56posuffadh5a367n5vi4uq 120 29 , , , work_sny56posuffadh5a367n5vi4uq 120 30 gap gap NN work_sny56posuffadh5a367n5vi4uq 120 31 / / SYM work_sny56posuffadh5a367n5vi4uq 120 32 insertion insertion NN work_sny56posuffadh5a367n5vi4uq 120 33 . . . work_sny56posuffadh5a367n5vi4uq 121 1 Canon- Canon- NNP work_sny56posuffadh5a367n5vi4uq 121 2 ical ical JJ work_sny56posuffadh5a367n5vi4uq 121 3 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 121 4 had have VBD work_sny56posuffadh5a367n5vi4uq 121 5 7-bp 7-bp CD work_sny56posuffadh5a367n5vi4uq 121 6 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 121 7 TACAGTA TACAGTA NNP work_sny56posuffadh5a367n5vi4uq 121 8 or or CC work_sny56posuffadh5a367n5vi4uq 121 9 TACT- TACT- NNP work_sny56posuffadh5a367n5vi4uq 121 10 GTA GTA NNP work_sny56posuffadh5a367n5vi4uq 121 11 conforming conform VBG work_sny56posuffadh5a367n5vi4uq 121 12 to to IN work_sny56posuffadh5a367n5vi4uq 121 13 the the DT work_sny56posuffadh5a367n5vi4uq 121 14 model model NN work_sny56posuffadh5a367n5vi4uq 121 15 illustrated illustrate VBN work_sny56posuffadh5a367n5vi4uq 121 16 in in IN work_sny56posuffadh5a367n5vi4uq 121 17 Figure Figure NNP work_sny56posuffadh5a367n5vi4uq 121 18 1B 1b NN work_sny56posuffadh5a367n5vi4uq 121 19 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 121 20 5-bp 5-bp JJ work_sny56posuffadh5a367n5vi4uq 121 21 insertions insertion NNS work_sny56posuffadh5a367n5vi4uq 121 22 of of IN work_sny56posuffadh5a367n5vi4uq 121 23 either either DT work_sny56posuffadh5a367n5vi4uq 121 24 CAGTA CAGTA NNP work_sny56posuffadh5a367n5vi4uq 121 25 or or CC work_sny56posuffadh5a367n5vi4uq 121 26 CTGTA CTGTA NNP work_sny56posuffadh5a367n5vi4uq 121 27 following follow VBG work_sny56posuffadh5a367n5vi4uq 121 28 Copyright copyright NN work_sny56posuffadh5a367n5vi4uq 121 29   CD work_sny56posuffadh5a367n5vi4uq 121 30 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 121 31 John John NNP work_sny56posuffadh5a367n5vi4uq 121 32 Wiley Wiley NNP work_sny56posuffadh5a367n5vi4uq 121 33 & & CC work_sny56posuffadh5a367n5vi4uq 121 34 Sons Sons NNPS work_sny56posuffadh5a367n5vi4uq 121 35 , , , work_sny56posuffadh5a367n5vi4uq 121 36 Ltd. Ltd. NNP work_sny56posuffadh5a367n5vi4uq 121 37 J J NNP work_sny56posuffadh5a367n5vi4uq 121 38 Gene Gene NNP work_sny56posuffadh5a367n5vi4uq 121 39 Med Med NNP work_sny56posuffadh5a367n5vi4uq 121 40 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 121 41 ; ; : work_sny56posuffadh5a367n5vi4uq 121 42 6 6 CD work_sny56posuffadh5a367n5vi4uq 121 43 : : : work_sny56posuffadh5a367n5vi4uq 121 44 574 574 CD work_sny56posuffadh5a367n5vi4uq 121 45 – – : work_sny56posuffadh5a367n5vi4uq 121 46 583 583 CD work_sny56posuffadh5a367n5vi4uq 121 47 . . . work_sny56posuffadh5a367n5vi4uq 122 1 578 578 CD work_sny56posuffadh5a367n5vi4uq 122 2 G. G. NNP work_sny56posuffadh5a367n5vi4uq 122 3 Liu Liu NNP work_sny56posuffadh5a367n5vi4uq 122 4 et et NNP work_sny56posuffadh5a367n5vi4uq 122 5 al al NNP work_sny56posuffadh5a367n5vi4uq 122 6 . . . work_sny56posuffadh5a367n5vi4uq 123 1 Table table NN work_sny56posuffadh5a367n5vi4uq 123 2 1 1 CD work_sny56posuffadh5a367n5vi4uq 123 3 . . . work_sny56posuffadh5a367n5vi4uq 124 1 Footprint footprint NN work_sny56posuffadh5a367n5vi4uq 124 2 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 124 3 from from IN work_sny56posuffadh5a367n5vi4uq 124 4 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 124 5 cells cell NNS work_sny56posuffadh5a367n5vi4uq 124 6 and and CC work_sny56posuffadh5a367n5vi4uq 124 7 zebrafish zebrafish JJ work_sny56posuffadh5a367n5vi4uq 124 8 embryos embryo NNS work_sny56posuffadh5a367n5vi4uq 124 9 Systems Systems NNPS work_sny56posuffadh5a367n5vi4uq 124 10 Category Category NNP work_sny56posuffadh5a367n5vi4uq 124 11 Left left JJ work_sny56posuffadh5a367n5vi4uq 124 12 - - HYPH work_sny56posuffadh5a367n5vi4uq 124 13 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 124 14 Footprint Footprint NNP work_sny56posuffadh5a367n5vi4uq 124 15 Right right RB work_sny56posuffadh5a367n5vi4uq 124 16 - - HYPH work_sny56posuffadh5a367n5vi4uq 124 17 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 124 18 Events event NNS work_sny56posuffadh5a367n5vi4uq 124 19 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 124 20 cells cell VBZ work_sny56posuffadh5a367n5vi4uq 124 21 Canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 124 22 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 124 23 TTCGAGCTCGGTACCC ttcgagctcggtaccc NN work_sny56posuffadh5a367n5vi4uq 124 24 TA TA NNP work_sny56posuffadh5a367n5vi4uq 124 25 CAG CAG NNP work_sny56posuffadh5a367n5vi4uq 124 26 TA TA NNP work_sny56posuffadh5a367n5vi4uq 124 27 GGGGATCCTCTAGAGT GGGGATCCTCTAGAGT NNP work_sny56posuffadh5a367n5vi4uq 124 28 4 4 CD work_sny56posuffadh5a367n5vi4uq 124 29 TTCGAGCTCGGTACCC ttcgagctcggtaccc NN work_sny56posuffadh5a367n5vi4uq 124 30 TA TA NNP work_sny56posuffadh5a367n5vi4uq 124 31 CTG CTG NNP work_sny56posuffadh5a367n5vi4uq 124 32 TA ta NN work_sny56posuffadh5a367n5vi4uq 124 33 GGGGATCCTCTAGAGT GGGGATCCTCTAGAGT NNP work_sny56posuffadh5a367n5vi4uq 124 34 2 2 CD work_sny56posuffadh5a367n5vi4uq 124 35 Non non JJ work_sny56posuffadh5a367n5vi4uq 124 36 - - JJ work_sny56posuffadh5a367n5vi4uq 124 37 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 124 38 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 124 39 TTCGAGCTCGGTACCC ttcgagctcggtaccc NN work_sny56posuffadh5a367n5vi4uq 124 40 T t NN work_sny56posuffadh5a367n5vi4uq 124 41 G g NN work_sny56posuffadh5a367n5vi4uq 124 42 TA ta NN work_sny56posuffadh5a367n5vi4uq 124 43 GGGGATCCTCTAGAGT GGGGATCCTCTAGAGT NNP work_sny56posuffadh5a367n5vi4uq 124 44 3 3 CD work_sny56posuffadh5a367n5vi4uq 124 45 TTCGAGCTCGGTACCC ttcgagctcggtaccc NN work_sny56posuffadh5a367n5vi4uq 124 46 TA ta NN work_sny56posuffadh5a367n5vi4uq 124 47 TA ta NN work_sny56posuffadh5a367n5vi4uq 124 48 GGGGATCCTCTAGAGT GGGGATCCTCTAGAGT NNP work_sny56posuffadh5a367n5vi4uq 124 49 2 2 CD work_sny56posuffadh5a367n5vi4uq 124 50 TTCGAGCTCGGTACCC ttcgagctcggtaccc NN work_sny56posuffadh5a367n5vi4uq 124 51 TA ta NN work_sny56posuffadh5a367n5vi4uq 124 52 C c NN work_sny56posuffadh5a367n5vi4uq 124 53 TA ta NN work_sny56posuffadh5a367n5vi4uq 124 54 GGGGATCCTCTAGAGT GGGGATCCTCTAGAGT NNP work_sny56posuffadh5a367n5vi4uq 124 55 1 1 CD work_sny56posuffadh5a367n5vi4uq 124 56 TTCGAGCTCGGTACCC ttcgagctcggtaccc NN work_sny56posuffadh5a367n5vi4uq 124 57 T t NN work_sny56posuffadh5a367n5vi4uq 124 58 TG tg NN work_sny56posuffadh5a367n5vi4uq 124 59 TA ta NN work_sny56posuffadh5a367n5vi4uq 124 60 GGGGATCCTCTAGAGT ggggatcctctagagt NN work_sny56posuffadh5a367n5vi4uq 124 61 1 1 CD work_sny56posuffadh5a367n5vi4uq 124 62 TTCGAGCTCGGTACCC ttcgagctcggtaccc NN work_sny56posuffadh5a367n5vi4uq 124 63 TA ta NN work_sny56posuffadh5a367n5vi4uq 124 64 C C NNP work_sny56posuffadh5a367n5vi4uq 124 65 A a NN work_sny56posuffadh5a367n5vi4uq 124 66 GGGGATCCTCTAGAGT GGGGATCCTCTAGAGT NNP work_sny56posuffadh5a367n5vi4uq 124 67 1 1 CD work_sny56posuffadh5a367n5vi4uq 124 68 Gaps gap NNS work_sny56posuffadh5a367n5vi4uq 124 69 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 124 70 82-bp 82-bp : work_sny56posuffadh5a367n5vi4uq 124 71 deletion deletion NN work_sny56posuffadh5a367n5vi4uq 124 72 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 124 73 G g NN work_sny56posuffadh5a367n5vi4uq 124 74 TA ta NN work_sny56posuffadh5a367n5vi4uq 124 75 GGGGATCCTCTAGAGT ggggatcctctagagt NN work_sny56posuffadh5a367n5vi4uq 124 76 1 1 CD work_sny56posuffadh5a367n5vi4uq 124 77 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 124 78 67-bp 67-bp CD work_sny56posuffadh5a367n5vi4uq 124 79 deletion deletion NN work_sny56posuffadh5a367n5vi4uq 124 80 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 124 81 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 124 82 89bp 89bp JJ work_sny56posuffadh5a367n5vi4uq 124 83 deletion deletion NN work_sny56posuffadh5a367n5vi4uq 124 84 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 124 85 1 1 CD work_sny56posuffadh5a367n5vi4uq 124 86 Insertions insertion NNS work_sny56posuffadh5a367n5vi4uq 124 87 TTCGAGCT ttcgagct NN work_sny56posuffadh5a367n5vi4uq 124 88 TGCATGTGGGAGGTTTTTTC TGCATGTGGGAGGTTTTTTC JJR work_sny56posuffadh5a367n5vi4uq 124 89 GGATCCTCTANAGT GGATCCTCTANAGT NNS work_sny56posuffadh5a367n5vi4uq 124 90 1 1 CD work_sny56posuffadh5a367n5vi4uq 124 91 Zebrafish zebrafish JJ work_sny56posuffadh5a367n5vi4uq 124 92 embryos embryo NNS work_sny56posuffadh5a367n5vi4uq 124 93 Canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 124 94 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 124 95 TTCGAGCTCGGTACCC ttcgagctcggtaccc NN work_sny56posuffadh5a367n5vi4uq 124 96 TA TA NNP work_sny56posuffadh5a367n5vi4uq 124 97 CTG CTG NNP work_sny56posuffadh5a367n5vi4uq 124 98 TA TA NNP work_sny56posuffadh5a367n5vi4uq 124 99 GGGGATCCTCTAGAGT GGGGATCCTCTAGAGT NNP work_sny56posuffadh5a367n5vi4uq 124 100 5 5 CD work_sny56posuffadh5a367n5vi4uq 124 101 TTCCAACNCGGTACCC TTCCAACNCGGTACCC NNP work_sny56posuffadh5a367n5vi4uq 124 102 TA TA NNP work_sny56posuffadh5a367n5vi4uq 124 103 CAG CAG NNP work_sny56posuffadh5a367n5vi4uq 124 104 TA TA NNP work_sny56posuffadh5a367n5vi4uq 124 105 GGGAATCCTCTAGAGT GGGAATCCTCTAGAGT NNP work_sny56posuffadh5a367n5vi4uq 124 106 4 4 CD work_sny56posuffadh5a367n5vi4uq 124 107 Gaps gap NNS work_sny56posuffadh5a367n5vi4uq 124 108 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 124 109 17bp 17bp JJ work_sny56posuffadh5a367n5vi4uq 124 110 deletion deletion NN work_sny56posuffadh5a367n5vi4uq 124 111 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 124 112 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 124 113 19bp 19bp JJ work_sny56posuffadh5a367n5vi4uq 124 114 deletion deletion NN work_sny56posuffadh5a367n5vi4uq 124 115 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 124 116 1 1 CD work_sny56posuffadh5a367n5vi4uq 124 117 Excision excision NN work_sny56posuffadh5a367n5vi4uq 124 118 sites site NNS work_sny56posuffadh5a367n5vi4uq 124 119 were be VBD work_sny56posuffadh5a367n5vi4uq 124 120 sequenced sequence VBN work_sny56posuffadh5a367n5vi4uq 124 121 using use VBG work_sny56posuffadh5a367n5vi4uq 124 122 primers primer NNS work_sny56posuffadh5a367n5vi4uq 124 123 outside outside IN work_sny56posuffadh5a367n5vi4uq 124 124 the the DT work_sny56posuffadh5a367n5vi4uq 124 125 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 124 126 . . . work_sny56posuffadh5a367n5vi4uq 125 1 Short short JJ work_sny56posuffadh5a367n5vi4uq 125 2 lines line NNS work_sny56posuffadh5a367n5vi4uq 125 3 indicate indicate VBP work_sny56posuffadh5a367n5vi4uq 125 4 the the DT work_sny56posuffadh5a367n5vi4uq 125 5 lost lost JJ work_sny56posuffadh5a367n5vi4uq 125 6 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 125 7 . . . work_sny56posuffadh5a367n5vi4uq 126 1 Footprint footprint NN work_sny56posuffadh5a367n5vi4uq 126 2 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 126 3 are be VBP work_sny56posuffadh5a367n5vi4uq 126 4 organized organize VBN work_sny56posuffadh5a367n5vi4uq 126 5 in in IN work_sny56posuffadh5a367n5vi4uq 126 6 several several JJ work_sny56posuffadh5a367n5vi4uq 126 7 categories category NNS work_sny56posuffadh5a367n5vi4uq 126 8 . . . work_sny56posuffadh5a367n5vi4uq 127 1 Canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 127 2 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 127 3 have have VBP work_sny56posuffadh5a367n5vi4uq 127 4 7-bp 7-bp CD work_sny56posuffadh5a367n5vi4uq 127 5 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 127 6 of of IN work_sny56posuffadh5a367n5vi4uq 127 7 TACAGTA tacagta NN work_sny56posuffadh5a367n5vi4uq 127 8 or or CC work_sny56posuffadh5a367n5vi4uq 127 9 TACTGTA tactgta NN work_sny56posuffadh5a367n5vi4uq 127 10 , , , work_sny56posuffadh5a367n5vi4uq 127 11 conforming conform VBG work_sny56posuffadh5a367n5vi4uq 127 12 to to IN work_sny56posuffadh5a367n5vi4uq 127 13 the the DT work_sny56posuffadh5a367n5vi4uq 127 14 model model NN work_sny56posuffadh5a367n5vi4uq 127 15 illustrated illustrate VBN work_sny56posuffadh5a367n5vi4uq 127 16 in in IN work_sny56posuffadh5a367n5vi4uq 127 17 Figure Figure NNP work_sny56posuffadh5a367n5vi4uq 127 18 1B. 1b. CD work_sny56posuffadh5a367n5vi4uq 128 1 Non non JJ work_sny56posuffadh5a367n5vi4uq 128 2 - - JJ work_sny56posuffadh5a367n5vi4uq 128 3 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 128 4 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 128 5 have have VBP work_sny56posuffadh5a367n5vi4uq 128 6 small small JJ work_sny56posuffadh5a367n5vi4uq 128 7 deletions deletion NNS work_sny56posuffadh5a367n5vi4uq 128 8 in in IN work_sny56posuffadh5a367n5vi4uq 128 9 the the DT work_sny56posuffadh5a367n5vi4uq 128 10 7-bp 7-bp JJ work_sny56posuffadh5a367n5vi4uq 128 11 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 128 12 sequence sequence NN work_sny56posuffadh5a367n5vi4uq 128 13 . . . work_sny56posuffadh5a367n5vi4uq 129 1 Gaps gap NNS work_sny56posuffadh5a367n5vi4uq 129 2 have have VBP work_sny56posuffadh5a367n5vi4uq 129 3 larger large JJR work_sny56posuffadh5a367n5vi4uq 129 4 deletions deletion NNS work_sny56posuffadh5a367n5vi4uq 129 5 in in IN work_sny56posuffadh5a367n5vi4uq 129 6 either either DT work_sny56posuffadh5a367n5vi4uq 129 7 or or CC work_sny56posuffadh5a367n5vi4uq 129 8 both both DT work_sny56posuffadh5a367n5vi4uq 129 9 sides side NNS work_sny56posuffadh5a367n5vi4uq 129 10 of of IN work_sny56posuffadh5a367n5vi4uq 129 11 the the DT work_sny56posuffadh5a367n5vi4uq 129 12 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 129 13 sequence sequence NN work_sny56posuffadh5a367n5vi4uq 129 14 as as IN work_sny56posuffadh5a367n5vi4uq 129 15 indicated indicate VBN work_sny56posuffadh5a367n5vi4uq 129 16 . . . work_sny56posuffadh5a367n5vi4uq 130 1 C C NNP work_sny56posuffadh5a367n5vi4uq 130 2 B B NNP work_sny56posuffadh5a367n5vi4uq 130 3 A A NNP work_sny56posuffadh5a367n5vi4uq 130 4 Figure figure NN work_sny56posuffadh5a367n5vi4uq 130 5 3 3 CD work_sny56posuffadh5a367n5vi4uq 130 6 . . . work_sny56posuffadh5a367n5vi4uq 131 1 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 131 2 analysis analysis NN work_sny56posuffadh5a367n5vi4uq 131 3 of of IN work_sny56posuffadh5a367n5vi4uq 131 4 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 131 5 excision excision NN work_sny56posuffadh5a367n5vi4uq 131 6 from from IN work_sny56posuffadh5a367n5vi4uq 131 7 plasmids plasmid NNS work_sny56posuffadh5a367n5vi4uq 131 8 in in IN work_sny56posuffadh5a367n5vi4uq 131 9 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 131 10 cells cell NNS work_sny56posuffadh5a367n5vi4uq 131 11 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 131 12 A a NN work_sny56posuffadh5a367n5vi4uq 131 13 and and CC work_sny56posuffadh5a367n5vi4uq 131 14 B b NN work_sny56posuffadh5a367n5vi4uq 131 15 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 131 16 and and CC work_sny56posuffadh5a367n5vi4uq 131 17 in in IN work_sny56posuffadh5a367n5vi4uq 131 18 zebrafish zebrafish NNP work_sny56posuffadh5a367n5vi4uq 131 19 embryos embryos NNP work_sny56posuffadh5a367n5vi4uq 131 20 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 131 21 C C NNP work_sny56posuffadh5a367n5vi4uq 131 22 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 131 23 . . . work_sny56posuffadh5a367n5vi4uq 132 1 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 132 2 A a DT work_sny56posuffadh5a367n5vi4uq 132 3 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 132 4 Plasmids Plasmids NNP work_sny56posuffadh5a367n5vi4uq 132 5 with with IN work_sny56posuffadh5a367n5vi4uq 132 6 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 132 7 and and CC work_sny56posuffadh5a367n5vi4uq 132 8 CMVSB cmvsb NN work_sny56posuffadh5a367n5vi4uq 132 9 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 132 10 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 132 11 pT pT NNP work_sny56posuffadh5a367n5vi4uq 132 12 / / SYM work_sny56posuffadh5a367n5vi4uq 132 13 neo neo NNP work_sny56posuffadh5a367n5vi4uq 132 14 and and CC work_sny56posuffadh5a367n5vi4uq 132 15 pCMVSB pcmvsb NN work_sny56posuffadh5a367n5vi4uq 132 16 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 132 17 were be VBD work_sny56posuffadh5a367n5vi4uq 132 18 co co VBN work_sny56posuffadh5a367n5vi4uq 132 19 - - VBN work_sny56posuffadh5a367n5vi4uq 132 20 transfected transfected JJ work_sny56posuffadh5a367n5vi4uq 132 21 into into IN work_sny56posuffadh5a367n5vi4uq 132 22 the the DT work_sny56posuffadh5a367n5vi4uq 132 23 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 132 24 cells cell NNS work_sny56posuffadh5a367n5vi4uq 132 25 . . . work_sny56posuffadh5a367n5vi4uq 133 1 Cell cell NN work_sny56posuffadh5a367n5vi4uq 133 2 lysate lysate NN work_sny56posuffadh5a367n5vi4uq 133 3 was be VBD work_sny56posuffadh5a367n5vi4uq 133 4 obtained obtain VBN work_sny56posuffadh5a367n5vi4uq 133 5 for for IN work_sny56posuffadh5a367n5vi4uq 133 6 nested nest VBN work_sny56posuffadh5a367n5vi4uq 133 7 - - HYPH work_sny56posuffadh5a367n5vi4uq 133 8 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 133 9 using use VBG work_sny56posuffadh5a367n5vi4uq 133 10 primers primer NNS work_sny56posuffadh5a367n5vi4uq 133 11 outside outside IN work_sny56posuffadh5a367n5vi4uq 133 12 the the DT work_sny56posuffadh5a367n5vi4uq 133 13 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 133 14 . . . work_sny56posuffadh5a367n5vi4uq 134 1 � � NNP work_sny56posuffadh5a367n5vi4uq 134 2 DDE DDE NNP work_sny56posuffadh5a367n5vi4uq 134 3 is be VBZ work_sny56posuffadh5a367n5vi4uq 134 4 a a DT work_sny56posuffadh5a367n5vi4uq 134 5 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 134 6 without without IN work_sny56posuffadh5a367n5vi4uq 134 7 a a DT work_sny56posuffadh5a367n5vi4uq 134 8 catalytic catalytic JJ work_sny56posuffadh5a367n5vi4uq 134 9 domain domain NN work_sny56posuffadh5a367n5vi4uq 134 10 . . . work_sny56posuffadh5a367n5vi4uq 135 1 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 135 2 B b NN work_sny56posuffadh5a367n5vi4uq 135 3 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 135 4 Time time NN work_sny56posuffadh5a367n5vi4uq 135 5 - - HYPH work_sny56posuffadh5a367n5vi4uq 135 6 course course NN work_sny56posuffadh5a367n5vi4uq 135 7 accumulation accumulation NN work_sny56posuffadh5a367n5vi4uq 135 8 of of IN work_sny56posuffadh5a367n5vi4uq 135 9 excision excision NN work_sny56posuffadh5a367n5vi4uq 135 10 products product NNS work_sny56posuffadh5a367n5vi4uq 135 11 from from IN work_sny56posuffadh5a367n5vi4uq 135 12 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 135 13 cells cell NNS work_sny56posuffadh5a367n5vi4uq 135 14 . . . work_sny56posuffadh5a367n5vi4uq 136 1 Hours hour NNS work_sny56posuffadh5a367n5vi4uq 136 2 post post NN work_sny56posuffadh5a367n5vi4uq 136 3 - - JJ work_sny56posuffadh5a367n5vi4uq 136 4 transfection transfection NN work_sny56posuffadh5a367n5vi4uq 136 5 are be VBP work_sny56posuffadh5a367n5vi4uq 136 6 marked mark VBN work_sny56posuffadh5a367n5vi4uq 136 7 on on IN work_sny56posuffadh5a367n5vi4uq 136 8 top top NN work_sny56posuffadh5a367n5vi4uq 136 9 of of IN work_sny56posuffadh5a367n5vi4uq 136 10 the the DT work_sny56posuffadh5a367n5vi4uq 136 11 gel gel NN work_sny56posuffadh5a367n5vi4uq 136 12 . . . work_sny56posuffadh5a367n5vi4uq 137 1 Each each DT work_sny56posuffadh5a367n5vi4uq 137 2 time time NN work_sny56posuffadh5a367n5vi4uq 137 3 point point NN work_sny56posuffadh5a367n5vi4uq 137 4 is be VBZ work_sny56posuffadh5a367n5vi4uq 137 5 represented represent VBN work_sny56posuffadh5a367n5vi4uq 137 6 by by IN work_sny56posuffadh5a367n5vi4uq 137 7 two two CD work_sny56posuffadh5a367n5vi4uq 137 8 separate separate JJ work_sny56posuffadh5a367n5vi4uq 137 9 transfections transfection NNS work_sny56posuffadh5a367n5vi4uq 137 10 . . . work_sny56posuffadh5a367n5vi4uq 138 1 The the DT work_sny56posuffadh5a367n5vi4uq 138 2 marker marker NN work_sny56posuffadh5a367n5vi4uq 138 3 lane lane NN work_sny56posuffadh5a367n5vi4uq 138 4 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 138 5 M m NN work_sny56posuffadh5a367n5vi4uq 138 6 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 138 7 on on IN work_sny56posuffadh5a367n5vi4uq 138 8 the the DT work_sny56posuffadh5a367n5vi4uq 138 9 left left NN work_sny56posuffadh5a367n5vi4uq 138 10 of of IN work_sny56posuffadh5a367n5vi4uq 138 11 the the DT work_sny56posuffadh5a367n5vi4uq 138 12 gels gel NNS work_sny56posuffadh5a367n5vi4uq 138 13 in in IN work_sny56posuffadh5a367n5vi4uq 138 14 A a NN work_sny56posuffadh5a367n5vi4uq 138 15 and and CC work_sny56posuffadh5a367n5vi4uq 138 16 C C NNP work_sny56posuffadh5a367n5vi4uq 138 17 is be VBZ work_sny56posuffadh5a367n5vi4uq 138 18 a a DT work_sny56posuffadh5a367n5vi4uq 138 19 100-bp 100-bp CD work_sny56posuffadh5a367n5vi4uq 138 20 ladder ladder NN work_sny56posuffadh5a367n5vi4uq 138 21 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 138 22 New New NNP work_sny56posuffadh5a367n5vi4uq 138 23 England England NNP work_sny56posuffadh5a367n5vi4uq 138 24 Biolabs Biolabs NNP work_sny56posuffadh5a367n5vi4uq 138 25 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 138 26 . . . work_sny56posuffadh5a367n5vi4uq 139 1 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 139 2 C C NNP work_sny56posuffadh5a367n5vi4uq 139 3 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 139 4 SB SB NNP work_sny56posuffadh5a367n5vi4uq 139 5 mRNA mRNA NNP work_sny56posuffadh5a367n5vi4uq 139 6 was be VBD work_sny56posuffadh5a367n5vi4uq 139 7 co co VBN work_sny56posuffadh5a367n5vi4uq 139 8 - - VBN work_sny56posuffadh5a367n5vi4uq 139 9 injected inject VBN work_sny56posuffadh5a367n5vi4uq 139 10 into into IN work_sny56posuffadh5a367n5vi4uq 139 11 one one CD work_sny56posuffadh5a367n5vi4uq 139 12 - - HYPH work_sny56posuffadh5a367n5vi4uq 139 13 cell cell NN work_sny56posuffadh5a367n5vi4uq 139 14 - - HYPH work_sny56posuffadh5a367n5vi4uq 139 15 stage stage NN work_sny56posuffadh5a367n5vi4uq 139 16 zebrafish zebrafish JJ work_sny56posuffadh5a367n5vi4uq 139 17 embryos embryo NNS work_sny56posuffadh5a367n5vi4uq 139 18 with with IN work_sny56posuffadh5a367n5vi4uq 139 19 plasmids plasmid NNS work_sny56posuffadh5a367n5vi4uq 139 20 containing contain VBG work_sny56posuffadh5a367n5vi4uq 139 21 a a DT work_sny56posuffadh5a367n5vi4uq 139 22 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 139 23 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 139 24 pT pT NNP work_sny56posuffadh5a367n5vi4uq 139 25 / / SYM work_sny56posuffadh5a367n5vi4uq 139 26 neo neo NNP work_sny56posuffadh5a367n5vi4uq 139 27 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 139 28 . . . work_sny56posuffadh5a367n5vi4uq 140 1 Twenty twenty CD work_sny56posuffadh5a367n5vi4uq 140 2 - - HYPH work_sny56posuffadh5a367n5vi4uq 140 3 four four CD work_sny56posuffadh5a367n5vi4uq 140 4 hours hour NNS work_sny56posuffadh5a367n5vi4uq 140 5 after after IN work_sny56posuffadh5a367n5vi4uq 140 6 microinjection microinjection NN work_sny56posuffadh5a367n5vi4uq 140 7 , , , work_sny56posuffadh5a367n5vi4uq 140 8 lysates lysate VBZ work_sny56posuffadh5a367n5vi4uq 140 9 from from IN work_sny56posuffadh5a367n5vi4uq 140 10 single single JJ work_sny56posuffadh5a367n5vi4uq 140 11 embryos embryo NNS work_sny56posuffadh5a367n5vi4uq 140 12 were be VBD work_sny56posuffadh5a367n5vi4uq 140 13 used use VBN work_sny56posuffadh5a367n5vi4uq 140 14 for for IN work_sny56posuffadh5a367n5vi4uq 140 15 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 140 16 analysis analysis NN work_sny56posuffadh5a367n5vi4uq 140 17 . . . work_sny56posuffadh5a367n5vi4uq 141 1 Two two CD work_sny56posuffadh5a367n5vi4uq 141 2 different different JJ work_sny56posuffadh5a367n5vi4uq 141 3 embryos embryo NNS work_sny56posuffadh5a367n5vi4uq 141 4 were be VBD work_sny56posuffadh5a367n5vi4uq 141 5 used use VBN work_sny56posuffadh5a367n5vi4uq 141 6 in in IN work_sny56posuffadh5a367n5vi4uq 141 7 the the DT work_sny56posuffadh5a367n5vi4uq 141 8 last last JJ work_sny56posuffadh5a367n5vi4uq 141 9 three three CD work_sny56posuffadh5a367n5vi4uq 141 10 categories category NNS work_sny56posuffadh5a367n5vi4uq 141 11 . . . work_sny56posuffadh5a367n5vi4uq 142 1 ∗ ∗ XX work_sny56posuffadh5a367n5vi4uq 142 2 : : : work_sny56posuffadh5a367n5vi4uq 142 3 pT pT NNP work_sny56posuffadh5a367n5vi4uq 142 4 / / SYM work_sny56posuffadh5a367n5vi4uq 142 5 neo neo NNP work_sny56posuffadh5a367n5vi4uq 142 6 plasmids plasmid NNS work_sny56posuffadh5a367n5vi4uq 142 7 mixed mix VBN work_sny56posuffadh5a367n5vi4uq 142 8 with with IN work_sny56posuffadh5a367n5vi4uq 142 9 embryo embryo NN work_sny56posuffadh5a367n5vi4uq 142 10 lysate lysate NN work_sny56posuffadh5a367n5vi4uq 142 11 were be VBD work_sny56posuffadh5a367n5vi4uq 142 12 used use VBN work_sny56posuffadh5a367n5vi4uq 142 13 as as IN work_sny56posuffadh5a367n5vi4uq 142 14 template template NN work_sny56posuffadh5a367n5vi4uq 142 15 in in IN work_sny56posuffadh5a367n5vi4uq 142 16 this this DT work_sny56posuffadh5a367n5vi4uq 142 17 case case NN work_sny56posuffadh5a367n5vi4uq 142 18 the the DT work_sny56posuffadh5a367n5vi4uq 142 19 insertion insertion NN work_sny56posuffadh5a367n5vi4uq 142 20 TA TA NNP work_sny56posuffadh5a367n5vi4uq 142 21 site site NN work_sny56posuffadh5a367n5vi4uq 142 22 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 142 23 . . . work_sny56posuffadh5a367n5vi4uq 143 1 Non non JJ work_sny56posuffadh5a367n5vi4uq 143 2 - - JJ work_sny56posuffadh5a367n5vi4uq 143 3 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 143 4 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 143 5 had have VBD work_sny56posuffadh5a367n5vi4uq 143 6 small small JJ work_sny56posuffadh5a367n5vi4uq 143 7 deletions deletion NNS work_sny56posuffadh5a367n5vi4uq 143 8 in in IN work_sny56posuffadh5a367n5vi4uq 143 9 the the DT work_sny56posuffadh5a367n5vi4uq 143 10 7-bp 7-bp JJ work_sny56posuffadh5a367n5vi4uq 143 11 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 143 12 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 143 13 . . . work_sny56posuffadh5a367n5vi4uq 144 1 In in IN work_sny56posuffadh5a367n5vi4uq 144 2 the the DT work_sny56posuffadh5a367n5vi4uq 144 3 case case NN work_sny56posuffadh5a367n5vi4uq 144 4 of of IN work_sny56posuffadh5a367n5vi4uq 144 5 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 144 6 cells cell NNS work_sny56posuffadh5a367n5vi4uq 144 7 , , , work_sny56posuffadh5a367n5vi4uq 144 8 deletions deletion NNS work_sny56posuffadh5a367n5vi4uq 144 9 were be VBD work_sny56posuffadh5a367n5vi4uq 144 10 usually usually RB work_sny56posuffadh5a367n5vi4uq 144 11 2 2 CD work_sny56posuffadh5a367n5vi4uq 144 12 – – SYM work_sny56posuffadh5a367n5vi4uq 144 13 3 3 CD work_sny56posuffadh5a367n5vi4uq 144 14 bp bp NN work_sny56posuffadh5a367n5vi4uq 144 15 . . . work_sny56posuffadh5a367n5vi4uq 145 1 Gaps gap NNS work_sny56posuffadh5a367n5vi4uq 145 2 had have VBD work_sny56posuffadh5a367n5vi4uq 145 3 large large JJ work_sny56posuffadh5a367n5vi4uq 145 4 deletions deletion NNS work_sny56posuffadh5a367n5vi4uq 145 5 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 145 6 17 17 CD work_sny56posuffadh5a367n5vi4uq 145 7 – – SYM work_sny56posuffadh5a367n5vi4uq 145 8 89 89 CD work_sny56posuffadh5a367n5vi4uq 145 9 bp bp NN work_sny56posuffadh5a367n5vi4uq 145 10 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 145 11 on on IN work_sny56posuffadh5a367n5vi4uq 145 12 either either DT work_sny56posuffadh5a367n5vi4uq 145 13 or or CC work_sny56posuffadh5a367n5vi4uq 145 14 both both DT work_sny56posuffadh5a367n5vi4uq 145 15 sides side NNS work_sny56posuffadh5a367n5vi4uq 145 16 of of IN work_sny56posuffadh5a367n5vi4uq 145 17 the the DT work_sny56posuffadh5a367n5vi4uq 145 18 flank- flank- NN work_sny56posuffadh5a367n5vi4uq 145 19 ing ing NN work_sny56posuffadh5a367n5vi4uq 145 20 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 145 21 . . . work_sny56posuffadh5a367n5vi4uq 146 1 In in IN work_sny56posuffadh5a367n5vi4uq 146 2 one one CD work_sny56posuffadh5a367n5vi4uq 146 3 case case NN work_sny56posuffadh5a367n5vi4uq 146 4 , , , work_sny56posuffadh5a367n5vi4uq 146 5 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 146 6 observed observe VBD work_sny56posuffadh5a367n5vi4uq 146 7 a a DT work_sny56posuffadh5a367n5vi4uq 146 8 20-bp 20-bp NNP work_sny56posuffadh5a367n5vi4uq 146 9 insertion insertion NN work_sny56posuffadh5a367n5vi4uq 146 10 of of IN work_sny56posuffadh5a367n5vi4uq 146 11 unknown unknown JJ work_sny56posuffadh5a367n5vi4uq 146 12 origin origin NN work_sny56posuffadh5a367n5vi4uq 146 13 between between IN work_sny56posuffadh5a367n5vi4uq 146 14 the the DT work_sny56posuffadh5a367n5vi4uq 146 15 two two CD work_sny56posuffadh5a367n5vi4uq 146 16 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 146 17 sites site NNS work_sny56posuffadh5a367n5vi4uq 146 18 . . . work_sny56posuffadh5a367n5vi4uq 147 1 Most Most JJS work_sny56posuffadh5a367n5vi4uq 147 2 of of IN work_sny56posuffadh5a367n5vi4uq 147 3 the the DT work_sny56posuffadh5a367n5vi4uq 147 4 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 147 5 in in IN work_sny56posuffadh5a367n5vi4uq 147 6 zebrafish zebrafish NNP work_sny56posuffadh5a367n5vi4uq 147 7 embryos embryos NNPS work_sny56posuffadh5a367n5vi4uq 147 8 were be VBD work_sny56posuffadh5a367n5vi4uq 147 9 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 147 10 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 147 11 , , , work_sny56posuffadh5a367n5vi4uq 147 12 whereas whereas IN work_sny56posuffadh5a367n5vi4uq 147 13 the the DT work_sny56posuffadh5a367n5vi4uq 147 14 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 147 15 in in IN work_sny56posuffadh5a367n5vi4uq 147 16 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 147 17 cells cell NNS work_sny56posuffadh5a367n5vi4uq 147 18 consisted consist VBN work_sny56posuffadh5a367n5vi4uq 147 19 of of IN work_sny56posuffadh5a367n5vi4uq 147 20 similar similar JJ work_sny56posuffadh5a367n5vi4uq 147 21 percentages percentage NNS work_sny56posuffadh5a367n5vi4uq 147 22 of of IN work_sny56posuffadh5a367n5vi4uq 147 23 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 147 24 and and CC work_sny56posuffadh5a367n5vi4uq 147 25 non non JJ work_sny56posuffadh5a367n5vi4uq 147 26 - - JJ work_sny56posuffadh5a367n5vi4uq 147 27 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 147 28 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 147 29 . . . work_sny56posuffadh5a367n5vi4uq 148 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 148 2 activity activity NN work_sny56posuffadh5a367n5vi4uq 148 3 correlates correlate VBZ work_sny56posuffadh5a367n5vi4uq 148 4 to to IN work_sny56posuffadh5a367n5vi4uq 148 5 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 148 6 activity activity NN work_sny56posuffadh5a367n5vi4uq 148 7 A a DT work_sny56posuffadh5a367n5vi4uq 148 8 preliminary preliminary JJ work_sny56posuffadh5a367n5vi4uq 148 9 study study NN work_sny56posuffadh5a367n5vi4uq 148 10 suggested suggest VBD work_sny56posuffadh5a367n5vi4uq 148 11 that that IN work_sny56posuffadh5a367n5vi4uq 148 12 the the DT work_sny56posuffadh5a367n5vi4uq 148 13 excision excision NN work_sny56posuffadh5a367n5vi4uq 148 14 activities activity NNS work_sny56posuffadh5a367n5vi4uq 148 15 correlate correlate VBP work_sny56posuffadh5a367n5vi4uq 148 16 to to IN work_sny56posuffadh5a367n5vi4uq 148 17 the the DT work_sny56posuffadh5a367n5vi4uq 148 18 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 148 19 activity activity NN work_sny56posuffadh5a367n5vi4uq 148 20 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 148 21 16 16 CD work_sny56posuffadh5a367n5vi4uq 148 22 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 148 23 . . . work_sny56posuffadh5a367n5vi4uq 149 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 149 2 examined examine VBD work_sny56posuffadh5a367n5vi4uq 149 3 this this DT work_sny56posuffadh5a367n5vi4uq 149 4 hypothesis hypothesis NN work_sny56posuffadh5a367n5vi4uq 149 5 by by IN work_sny56posuffadh5a367n5vi4uq 149 6 measuring measure VBG work_sny56posuffadh5a367n5vi4uq 149 7 the the DT work_sny56posuffadh5a367n5vi4uq 149 8 excision excision NN work_sny56posuffadh5a367n5vi4uq 149 9 activity activity NN work_sny56posuffadh5a367n5vi4uq 149 10 of of IN work_sny56posuffadh5a367n5vi4uq 149 11 IR IR NNP work_sny56posuffadh5a367n5vi4uq 149 12 / / SYM work_sny56posuffadh5a367n5vi4uq 149 13 DR DR NNP work_sny56posuffadh5a367n5vi4uq 149 14 mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 149 15 shown show VBN work_sny56posuffadh5a367n5vi4uq 149 16 to to TO work_sny56posuffadh5a367n5vi4uq 149 17 have have VB work_sny56posuffadh5a367n5vi4uq 149 18 different different JJ work_sny56posuffadh5a367n5vi4uq 149 19 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 149 20 rates rate NNS work_sny56posuffadh5a367n5vi4uq 149 21 to to TO work_sny56posuffadh5a367n5vi4uq 149 22 evaluate evaluate VB work_sny56posuffadh5a367n5vi4uq 149 23 its -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 149 24 validity validity NN work_sny56posuffadh5a367n5vi4uq 149 25 . . . work_sny56posuffadh5a367n5vi4uq 150 1 To to TO work_sny56posuffadh5a367n5vi4uq 150 2 quantify quantify VB work_sny56posuffadh5a367n5vi4uq 150 3 the the DT work_sny56posuffadh5a367n5vi4uq 150 4 levels level NNS work_sny56posuffadh5a367n5vi4uq 150 5 of of IN work_sny56posuffadh5a367n5vi4uq 150 6 excision excision NN work_sny56posuffadh5a367n5vi4uq 150 7 , , , work_sny56posuffadh5a367n5vi4uq 150 8 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 150 9 generated generate VBD work_sny56posuffadh5a367n5vi4uq 150 10 a a DT work_sny56posuffadh5a367n5vi4uq 150 11 set set NN work_sny56posuffadh5a367n5vi4uq 150 12 of of IN work_sny56posuffadh5a367n5vi4uq 150 13 standards standard NNS work_sny56posuffadh5a367n5vi4uq 150 14 using use VBG work_sny56posuffadh5a367n5vi4uq 150 15 dilutions dilution NNS work_sny56posuffadh5a367n5vi4uq 150 16 of of IN work_sny56posuffadh5a367n5vi4uq 150 17 the the DT work_sny56posuffadh5a367n5vi4uq 150 18 excision excision NN work_sny56posuffadh5a367n5vi4uq 150 19 lysate lysate NN work_sny56posuffadh5a367n5vi4uq 150 20 of of IN work_sny56posuffadh5a367n5vi4uq 150 21 the the DT work_sny56posuffadh5a367n5vi4uq 150 22 original original JJ work_sny56posuffadh5a367n5vi4uq 150 23 pT pT NNP work_sny56posuffadh5a367n5vi4uq 150 24 / / SYM work_sny56posuffadh5a367n5vi4uq 150 25 HindIIIneo hindiiineo NN work_sny56posuffadh5a367n5vi4uq 150 26 . . . work_sny56posuffadh5a367n5vi4uq 151 1 As as IN work_sny56posuffadh5a367n5vi4uq 151 2 the the DT work_sny56posuffadh5a367n5vi4uq 151 3 control control NN work_sny56posuffadh5a367n5vi4uq 151 4 for for IN work_sny56posuffadh5a367n5vi4uq 151 5 the the DT work_sny56posuffadh5a367n5vi4uq 151 6 total total JJ work_sny56posuffadh5a367n5vi4uq 151 7 input input NN work_sny56posuffadh5a367n5vi4uq 151 8 template template NN work_sny56posuffadh5a367n5vi4uq 151 9 , , , work_sny56posuffadh5a367n5vi4uq 151 10 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 151 11 used use VBD work_sny56posuffadh5a367n5vi4uq 151 12 the the DT work_sny56posuffadh5a367n5vi4uq 151 13 amplification amplification NN work_sny56posuffadh5a367n5vi4uq 151 14 of of IN work_sny56posuffadh5a367n5vi4uq 151 15 a a DT work_sny56posuffadh5a367n5vi4uq 151 16 segment segment NN work_sny56posuffadh5a367n5vi4uq 151 17 in in IN work_sny56posuffadh5a367n5vi4uq 151 18 the the DT work_sny56posuffadh5a367n5vi4uq 151 19 backbone backbone NN work_sny56posuffadh5a367n5vi4uq 151 20 of of IN work_sny56posuffadh5a367n5vi4uq 151 21 both both CC work_sny56posuffadh5a367n5vi4uq 151 22 pT pT NNP work_sny56posuffadh5a367n5vi4uq 151 23 and and CC work_sny56posuffadh5a367n5vi4uq 151 24 pCMVSB pcmvsb NN work_sny56posuffadh5a367n5vi4uq 151 25 plasmids plasmid NNS work_sny56posuffadh5a367n5vi4uq 151 26 . . . work_sny56posuffadh5a367n5vi4uq 152 1 For for IN work_sny56posuffadh5a367n5vi4uq 152 2 each each DT work_sny56posuffadh5a367n5vi4uq 152 3 gel gel NN work_sny56posuffadh5a367n5vi4uq 152 4 scan scan NNP work_sny56posuffadh5a367n5vi4uq 152 5 , , , work_sny56posuffadh5a367n5vi4uq 152 6 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 152 7 first first RB work_sny56posuffadh5a367n5vi4uq 152 8 standardized standardize VBD work_sny56posuffadh5a367n5vi4uq 152 9 the the DT work_sny56posuffadh5a367n5vi4uq 152 10 intensity intensity NN work_sny56posuffadh5a367n5vi4uq 152 11 of of IN work_sny56posuffadh5a367n5vi4uq 152 12 the the DT work_sny56posuffadh5a367n5vi4uq 152 13 excision excision NN work_sny56posuffadh5a367n5vi4uq 152 14 bands band NNS work_sny56posuffadh5a367n5vi4uq 152 15 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 152 16 in in IN work_sny56posuffadh5a367n5vi4uq 152 17 arbitrary arbitrary JJ work_sny56posuffadh5a367n5vi4uq 152 18 increments increment NNS work_sny56posuffadh5a367n5vi4uq 152 19 of of IN work_sny56posuffadh5a367n5vi4uq 152 20 density density NN work_sny56posuffadh5a367n5vi4uq 152 21 units unit NNS work_sny56posuffadh5a367n5vi4uq 152 22 , , , work_sny56posuffadh5a367n5vi4uq 152 23 IOD IOD NNP work_sny56posuffadh5a367n5vi4uq 152 24 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 152 25 by by IN work_sny56posuffadh5a367n5vi4uq 152 26 dividing divide VBG work_sny56posuffadh5a367n5vi4uq 152 27 the the DT work_sny56posuffadh5a367n5vi4uq 152 28 IOD IOD NNP work_sny56posuffadh5a367n5vi4uq 152 29 of of IN work_sny56posuffadh5a367n5vi4uq 152 30 the the DT work_sny56posuffadh5a367n5vi4uq 152 31 excision excision NN work_sny56posuffadh5a367n5vi4uq 152 32 bands band NNS work_sny56posuffadh5a367n5vi4uq 152 33 by by IN work_sny56posuffadh5a367n5vi4uq 152 34 the the DT work_sny56posuffadh5a367n5vi4uq 152 35 relative relative JJ work_sny56posuffadh5a367n5vi4uq 152 36 amount amount NN work_sny56posuffadh5a367n5vi4uq 152 37 of of IN work_sny56posuffadh5a367n5vi4uq 152 38 total total JJ work_sny56posuffadh5a367n5vi4uq 152 39 input input NN work_sny56posuffadh5a367n5vi4uq 152 40 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 152 41 . . . work_sny56posuffadh5a367n5vi4uq 153 1 A a DT work_sny56posuffadh5a367n5vi4uq 153 2 standard standard JJ work_sny56posuffadh5a367n5vi4uq 153 3 curve curve NN work_sny56posuffadh5a367n5vi4uq 153 4 was be VBD work_sny56posuffadh5a367n5vi4uq 153 5 generated generate VBN work_sny56posuffadh5a367n5vi4uq 153 6 from from IN work_sny56posuffadh5a367n5vi4uq 153 7 the the DT work_sny56posuffadh5a367n5vi4uq 153 8 standardized standardized JJ work_sny56posuffadh5a367n5vi4uq 153 9 IOD IOD NNP work_sny56posuffadh5a367n5vi4uq 153 10 values value NNS work_sny56posuffadh5a367n5vi4uq 153 11 of of IN work_sny56posuffadh5a367n5vi4uq 153 12 the the DT work_sny56posuffadh5a367n5vi4uq 153 13 amplification amplification NN work_sny56posuffadh5a367n5vi4uq 153 14 products product NNS work_sny56posuffadh5a367n5vi4uq 153 15 resulting result VBG work_sny56posuffadh5a367n5vi4uq 153 16 from from IN work_sny56posuffadh5a367n5vi4uq 153 17 dilutions dilution NNS work_sny56posuffadh5a367n5vi4uq 153 18 of of IN work_sny56posuffadh5a367n5vi4uq 153 19 the the DT work_sny56posuffadh5a367n5vi4uq 153 20 standard standard JJ work_sny56posuffadh5a367n5vi4uq 153 21 pT pT NNP work_sny56posuffadh5a367n5vi4uq 153 22 / / SYM work_sny56posuffadh5a367n5vi4uq 153 23 HindIIIneo hindiiineo NN work_sny56posuffadh5a367n5vi4uq 153 24 . . . work_sny56posuffadh5a367n5vi4uq 154 1 The the DT work_sny56posuffadh5a367n5vi4uq 154 2 relative relative JJ work_sny56posuffadh5a367n5vi4uq 154 3 excision excision NN work_sny56posuffadh5a367n5vi4uq 154 4 activity activity NN work_sny56posuffadh5a367n5vi4uq 154 5 for for IN work_sny56posuffadh5a367n5vi4uq 154 6 each each DT work_sny56posuffadh5a367n5vi4uq 154 7 reaction reaction NN work_sny56posuffadh5a367n5vi4uq 154 8 was be VBD work_sny56posuffadh5a367n5vi4uq 154 9 calculated calculate VBN work_sny56posuffadh5a367n5vi4uq 154 10 as as IN work_sny56posuffadh5a367n5vi4uq 154 11 a a DT work_sny56posuffadh5a367n5vi4uq 154 12 percentage percentage NN work_sny56posuffadh5a367n5vi4uq 154 13 of of IN work_sny56posuffadh5a367n5vi4uq 154 14 the the DT work_sny56posuffadh5a367n5vi4uq 154 15 pT pT NNP work_sny56posuffadh5a367n5vi4uq 154 16 / / SYM work_sny56posuffadh5a367n5vi4uq 154 17 HindIIIneo HindIIIneo NNP work_sny56posuffadh5a367n5vi4uq 154 18 activity activity NN work_sny56posuffadh5a367n5vi4uq 154 19 . . . work_sny56posuffadh5a367n5vi4uq 155 1 Figure figure NN work_sny56posuffadh5a367n5vi4uq 155 2 4 4 CD work_sny56posuffadh5a367n5vi4uq 155 3 shows show VBZ work_sny56posuffadh5a367n5vi4uq 155 4 one one CD work_sny56posuffadh5a367n5vi4uq 155 5 example example NN work_sny56posuffadh5a367n5vi4uq 155 6 of of IN work_sny56posuffadh5a367n5vi4uq 155 7 a a DT work_sny56posuffadh5a367n5vi4uq 155 8 gel gel NN work_sny56posuffadh5a367n5vi4uq 155 9 scan scan NNP work_sny56posuffadh5a367n5vi4uq 155 10 and and CC work_sny56posuffadh5a367n5vi4uq 155 11 the the DT work_sny56posuffadh5a367n5vi4uq 155 12 quantification quantification NN work_sny56posuffadh5a367n5vi4uq 155 13 of of IN work_sny56posuffadh5a367n5vi4uq 155 14 the the DT work_sny56posuffadh5a367n5vi4uq 155 15 excision excision NN work_sny56posuffadh5a367n5vi4uq 155 16 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 155 17 . . . work_sny56posuffadh5a367n5vi4uq 156 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 156 2 compared compare VBD work_sny56posuffadh5a367n5vi4uq 156 3 these these DT work_sny56posuffadh5a367n5vi4uq 156 4 relative relative JJ work_sny56posuffadh5a367n5vi4uq 156 5 excision excision NN work_sny56posuffadh5a367n5vi4uq 156 6 activities activity NNS work_sny56posuffadh5a367n5vi4uq 156 7 with with IN work_sny56posuffadh5a367n5vi4uq 156 8 the the DT work_sny56posuffadh5a367n5vi4uq 156 9 earlier early RBR work_sny56posuffadh5a367n5vi4uq 156 10 reported report VBN work_sny56posuffadh5a367n5vi4uq 156 11 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 156 12 rates rate NNS work_sny56posuffadh5a367n5vi4uq 156 13 measured measure VBN work_sny56posuffadh5a367n5vi4uq 156 14 by by IN work_sny56posuffadh5a367n5vi4uq 156 15 a a DT work_sny56posuffadh5a367n5vi4uq 156 16 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 156 17 assay assay NN work_sny56posuffadh5a367n5vi4uq 156 18 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 156 19 16 16 CD work_sny56posuffadh5a367n5vi4uq 156 20 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 156 21 . . . work_sny56posuffadh5a367n5vi4uq 157 1 In in IN work_sny56posuffadh5a367n5vi4uq 157 2 this this DT work_sny56posuffadh5a367n5vi4uq 157 3 assay assay NN work_sny56posuffadh5a367n5vi4uq 157 4 , , , work_sny56posuffadh5a367n5vi4uq 157 5 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 157 6 - - HYPH work_sny56posuffadh5a367n5vi4uq 157 7 mediated mediate VBN work_sny56posuffadh5a367n5vi4uq 157 8 chromosome chromosome NN work_sny56posuffadh5a367n5vi4uq 157 9 integration integration NN work_sny56posuffadh5a367n5vi4uq 157 10 was be VBD work_sny56posuffadh5a367n5vi4uq 157 11 indicated indicate VBN work_sny56posuffadh5a367n5vi4uq 157 12 by by IN work_sny56posuffadh5a367n5vi4uq 157 13 the the DT work_sny56posuffadh5a367n5vi4uq 157 14 increase increase NN work_sny56posuffadh5a367n5vi4uq 157 15 in in IN work_sny56posuffadh5a367n5vi4uq 157 16 the the DT work_sny56posuffadh5a367n5vi4uq 157 17 number number NN work_sny56posuffadh5a367n5vi4uq 157 18 of of IN work_sny56posuffadh5a367n5vi4uq 157 19 G418-resistant g418-resistant JJ work_sny56posuffadh5a367n5vi4uq 157 20 colonies colony NNS work_sny56posuffadh5a367n5vi4uq 157 21 , , , work_sny56posuffadh5a367n5vi4uq 157 22 and and CC work_sny56posuffadh5a367n5vi4uq 157 23 the the DT work_sny56posuffadh5a367n5vi4uq 157 24 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 157 25 rate rate NN work_sny56posuffadh5a367n5vi4uq 157 26 was be VBD work_sny56posuffadh5a367n5vi4uq 157 27 measured measure VBN work_sny56posuffadh5a367n5vi4uq 157 28 as as IN work_sny56posuffadh5a367n5vi4uq 157 29 the the DT work_sny56posuffadh5a367n5vi4uq 157 30 percentage percentage NN work_sny56posuffadh5a367n5vi4uq 157 31 of of IN work_sny56posuffadh5a367n5vi4uq 157 32 the the DT work_sny56posuffadh5a367n5vi4uq 157 33 pT pT NNP work_sny56posuffadh5a367n5vi4uq 157 34 / / SYM work_sny56posuffadh5a367n5vi4uq 157 35 HindIIIneo HindIIIneo NNP work_sny56posuffadh5a367n5vi4uq 157 36 activity activity NN work_sny56posuffadh5a367n5vi4uq 157 37 . . . work_sny56posuffadh5a367n5vi4uq 158 1 The the DT work_sny56posuffadh5a367n5vi4uq 158 2 comparisons comparison NNS work_sny56posuffadh5a367n5vi4uq 158 3 of of IN work_sny56posuffadh5a367n5vi4uq 158 4 the the DT work_sny56posuffadh5a367n5vi4uq 158 5 activities activity NNS work_sny56posuffadh5a367n5vi4uq 158 6 of of IN work_sny56posuffadh5a367n5vi4uq 158 7 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 158 8 and and CC work_sny56posuffadh5a367n5vi4uq 158 9 excision excision NN work_sny56posuffadh5a367n5vi4uq 158 10 are be VBP work_sny56posuffadh5a367n5vi4uq 158 11 summarized summarize VBN work_sny56posuffadh5a367n5vi4uq 158 12 in in IN work_sny56posuffadh5a367n5vi4uq 158 13 Table table NN work_sny56posuffadh5a367n5vi4uq 158 14 2 2 CD work_sny56posuffadh5a367n5vi4uq 158 15 . . . work_sny56posuffadh5a367n5vi4uq 159 1 Mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 159 2 with with IN work_sny56posuffadh5a367n5vi4uq 159 3 high high JJ work_sny56posuffadh5a367n5vi4uq 159 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 159 5 activities activity NNS work_sny56posuffadh5a367n5vi4uq 159 6 correlated correlate VBD work_sny56posuffadh5a367n5vi4uq 159 7 with with IN work_sny56posuffadh5a367n5vi4uq 159 8 high high JJ work_sny56posuffadh5a367n5vi4uq 159 9 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 159 10 activities activity NNS work_sny56posuffadh5a367n5vi4uq 159 11 Copyright copyright NN work_sny56posuffadh5a367n5vi4uq 159 12   CD work_sny56posuffadh5a367n5vi4uq 159 13 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 159 14 John John NNP work_sny56posuffadh5a367n5vi4uq 159 15 Wiley Wiley NNP work_sny56posuffadh5a367n5vi4uq 159 16 & & CC work_sny56posuffadh5a367n5vi4uq 159 17 Sons Sons NNPS work_sny56posuffadh5a367n5vi4uq 159 18 , , , work_sny56posuffadh5a367n5vi4uq 159 19 Ltd. Ltd. NNP work_sny56posuffadh5a367n5vi4uq 159 20 J J NNP work_sny56posuffadh5a367n5vi4uq 159 21 Gene Gene NNP work_sny56posuffadh5a367n5vi4uq 159 22 Med Med NNP work_sny56posuffadh5a367n5vi4uq 159 23 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 159 24 ; ; : work_sny56posuffadh5a367n5vi4uq 159 25 6 6 CD work_sny56posuffadh5a367n5vi4uq 159 26 : : : work_sny56posuffadh5a367n5vi4uq 159 27 574 574 CD work_sny56posuffadh5a367n5vi4uq 159 28 – – : work_sny56posuffadh5a367n5vi4uq 159 29 583 583 CD work_sny56posuffadh5a367n5vi4uq 159 30 . . . work_sny56posuffadh5a367n5vi4uq 160 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 160 2 of of IN work_sny56posuffadh5a367n5vi4uq 160 3 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 160 4 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 160 5 Transposons Transposons NNP work_sny56posuffadh5a367n5vi4uq 160 6 579 579 CD work_sny56posuffadh5a367n5vi4uq 160 7 and and CC work_sny56posuffadh5a367n5vi4uq 160 8 mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 160 9 with with IN work_sny56posuffadh5a367n5vi4uq 160 10 low low JJ work_sny56posuffadh5a367n5vi4uq 160 11 excision excision NN work_sny56posuffadh5a367n5vi4uq 160 12 activities activity NNS work_sny56posuffadh5a367n5vi4uq 160 13 correlated correlate VBD work_sny56posuffadh5a367n5vi4uq 160 14 with with IN work_sny56posuffadh5a367n5vi4uq 160 15 low low JJ work_sny56posuffadh5a367n5vi4uq 160 16 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 160 17 activities activity NNS work_sny56posuffadh5a367n5vi4uq 160 18 . . . work_sny56posuffadh5a367n5vi4uq 161 1 Although although IN work_sny56posuffadh5a367n5vi4uq 161 2 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 161 3 activities activity NNS work_sny56posuffadh5a367n5vi4uq 161 4 varied vary VBD work_sny56posuffadh5a367n5vi4uq 161 5 between between IN work_sny56posuffadh5a367n5vi4uq 161 6 parallel parallel JJ work_sny56posuffadh5a367n5vi4uq 161 7 experiments experiment NNS work_sny56posuffadh5a367n5vi4uq 161 8 , , , work_sny56posuffadh5a367n5vi4uq 161 9 the the DT work_sny56posuffadh5a367n5vi4uq 161 10 relative relative JJ work_sny56posuffadh5a367n5vi4uq 161 11 excision excision NN work_sny56posuffadh5a367n5vi4uq 161 12 activities activity NNS work_sny56posuffadh5a367n5vi4uq 161 13 approximated approximate VBD work_sny56posuffadh5a367n5vi4uq 161 14 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 161 15 rates rate NNS work_sny56posuffadh5a367n5vi4uq 161 16 within within IN work_sny56posuffadh5a367n5vi4uq 161 17 the the DT work_sny56posuffadh5a367n5vi4uq 161 18 uncertainties uncertainty NNS work_sny56posuffadh5a367n5vi4uq 161 19 of of IN work_sny56posuffadh5a367n5vi4uq 161 20 our -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 161 21 measurements measurement NNS work_sny56posuffadh5a367n5vi4uq 161 22 , , , work_sny56posuffadh5a367n5vi4uq 161 23 suggesting suggest VBG work_sny56posuffadh5a367n5vi4uq 161 24 that that IN work_sny56posuffadh5a367n5vi4uq 161 25 excision excision NN work_sny56posuffadh5a367n5vi4uq 161 26 can can MD work_sny56posuffadh5a367n5vi4uq 161 27 be be VB work_sny56posuffadh5a367n5vi4uq 161 28 used use VBN work_sny56posuffadh5a367n5vi4uq 161 29 as as IN work_sny56posuffadh5a367n5vi4uq 161 30 an an DT work_sny56posuffadh5a367n5vi4uq 161 31 indicator indicator NN work_sny56posuffadh5a367n5vi4uq 161 32 of of IN work_sny56posuffadh5a367n5vi4uq 161 33 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 161 34 efficiency efficiency NN work_sny56posuffadh5a367n5vi4uq 161 35 and and CC work_sny56posuffadh5a367n5vi4uq 161 36 thereby thereby RB work_sny56posuffadh5a367n5vi4uq 161 37 for for IN work_sny56posuffadh5a367n5vi4uq 161 38 improvement improvement NN work_sny56posuffadh5a367n5vi4uq 161 39 of of IN work_sny56posuffadh5a367n5vi4uq 161 40 the the DT work_sny56posuffadh5a367n5vi4uq 161 41 SB SB NNP work_sny56posuffadh5a367n5vi4uq 161 42 system system NN work_sny56posuffadh5a367n5vi4uq 161 43 . . . work_sny56posuffadh5a367n5vi4uq 162 1 Use use NN work_sny56posuffadh5a367n5vi4uq 162 2 of of IN work_sny56posuffadh5a367n5vi4uq 162 3 the the DT work_sny56posuffadh5a367n5vi4uq 162 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 162 5 assay assay NN work_sny56posuffadh5a367n5vi4uq 162 6 to to TO work_sny56posuffadh5a367n5vi4uq 162 7 improve improve VB work_sny56posuffadh5a367n5vi4uq 162 8 the the DT work_sny56posuffadh5a367n5vi4uq 162 9 SB SB NNP work_sny56posuffadh5a367n5vi4uq 162 10 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 162 11 system system NN work_sny56posuffadh5a367n5vi4uq 162 12 The the DT work_sny56posuffadh5a367n5vi4uq 162 13 outer outer JJ work_sny56posuffadh5a367n5vi4uq 162 14 and and CC work_sny56posuffadh5a367n5vi4uq 162 15 inner inner JJ work_sny56posuffadh5a367n5vi4uq 162 16 DRs dr NNS work_sny56posuffadh5a367n5vi4uq 162 17 have have VBP work_sny56posuffadh5a367n5vi4uq 162 18 different different JJ work_sny56posuffadh5a367n5vi4uq 162 19 roles role NNS work_sny56posuffadh5a367n5vi4uq 162 20 in in IN work_sny56posuffadh5a367n5vi4uq 162 21 excision excision NN work_sny56posuffadh5a367n5vi4uq 162 22 and and CC work_sny56posuffadh5a367n5vi4uq 162 23 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 162 24 can can MD work_sny56posuffadh5a367n5vi4uq 162 25 be be VB work_sny56posuffadh5a367n5vi4uq 162 26 excised excise VBN work_sny56posuffadh5a367n5vi4uq 162 27 efficiently efficiently RB work_sny56posuffadh5a367n5vi4uq 162 28 only only RB work_sny56posuffadh5a367n5vi4uq 162 29 at at IN work_sny56posuffadh5a367n5vi4uq 162 30 the the DT work_sny56posuffadh5a367n5vi4uq 162 31 outer outer JJ work_sny56posuffadh5a367n5vi4uq 162 32 DRs dr NNS work_sny56posuffadh5a367n5vi4uq 162 33 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 162 34 16 16 CD work_sny56posuffadh5a367n5vi4uq 162 35 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 162 36 . . . work_sny56posuffadh5a367n5vi4uq 163 1 These these DT work_sny56posuffadh5a367n5vi4uq 163 2 results result NNS work_sny56posuffadh5a367n5vi4uq 163 3 suggested suggest VBD work_sny56posuffadh5a367n5vi4uq 163 4 that that IN work_sny56posuffadh5a367n5vi4uq 163 5 it -PRON- PRP work_sny56posuffadh5a367n5vi4uq 163 6 is be VBZ work_sny56posuffadh5a367n5vi4uq 163 7 not not RB work_sny56posuffadh5a367n5vi4uq 163 8 the the DT work_sny56posuffadh5a367n5vi4uq 163 9 location location NN work_sny56posuffadh5a367n5vi4uq 163 10 but but CC work_sny56posuffadh5a367n5vi4uq 163 11 the the DT work_sny56posuffadh5a367n5vi4uq 163 12 differences difference NNS work_sny56posuffadh5a367n5vi4uq 163 13 in in IN work_sny56posuffadh5a367n5vi4uq 163 14 sequence sequence NN work_sny56posuffadh5a367n5vi4uq 163 15 between between IN work_sny56posuffadh5a367n5vi4uq 163 16 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 163 17 and and CC work_sny56posuffadh5a367n5vi4uq 163 18 Li Li NNP work_sny56posuffadh5a367n5vi4uq 163 19 that that WDT work_sny56posuffadh5a367n5vi4uq 163 20 gives give VBZ work_sny56posuffadh5a367n5vi4uq 163 21 them -PRON- PRP work_sny56posuffadh5a367n5vi4uq 163 22 different different JJ work_sny56posuffadh5a367n5vi4uq 163 23 functional functional JJ work_sny56posuffadh5a367n5vi4uq 163 24 roles role NNS work_sny56posuffadh5a367n5vi4uq 163 25 . . . work_sny56posuffadh5a367n5vi4uq 164 1 Comparison comparison NN work_sny56posuffadh5a367n5vi4uq 164 2 of of IN work_sny56posuffadh5a367n5vi4uq 164 3 the the DT work_sny56posuffadh5a367n5vi4uq 164 4 Table table NN work_sny56posuffadh5a367n5vi4uq 164 5 2 2 CD work_sny56posuffadh5a367n5vi4uq 164 6 . . . work_sny56posuffadh5a367n5vi4uq 165 1 Comparison comparison NN work_sny56posuffadh5a367n5vi4uq 165 2 of of IN work_sny56posuffadh5a367n5vi4uq 165 3 relative relative JJ work_sny56posuffadh5a367n5vi4uq 165 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 165 5 activity activity NN work_sny56posuffadh5a367n5vi4uq 165 6 and and CC work_sny56posuffadh5a367n5vi4uq 165 7 relative relative JJ work_sny56posuffadh5a367n5vi4uq 165 8 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 165 9 rates rate NNS work_sny56posuffadh5a367n5vi4uq 165 10 Constructs Constructs NNP work_sny56posuffadh5a367n5vi4uq 165 11 Relative relative JJ work_sny56posuffadh5a367n5vi4uq 165 12 excision excision NN work_sny56posuffadh5a367n5vi4uq 165 13 activity activity NN work_sny56posuffadh5a367n5vi4uq 165 14 Relative relative JJ work_sny56posuffadh5a367n5vi4uq 165 15 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 165 16 rate rate NN work_sny56posuffadh5a367n5vi4uq 165 17 LoLi LoLi NNP work_sny56posuffadh5a367n5vi4uq 165 18 − − NNP work_sny56posuffadh5a367n5vi4uq 165 19 RiRo RiRo NNP work_sny56posuffadh5a367n5vi4uq 165 20 + + SYM work_sny56posuffadh5a367n5vi4uq 165 21 � � NNP work_sny56posuffadh5a367n5vi4uq 165 22 DDE DDE NNP work_sny56posuffadh5a367n5vi4uq 165 23 nd nd NNP work_sny56posuffadh5a367n5vi4uq 165 24 4 4 CD work_sny56posuffadh5a367n5vi4uq 165 25 % % NN work_sny56posuffadh5a367n5vi4uq 165 26 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 165 27 1%)∗ 1%)∗ CD work_sny56posuffadh5a367n5vi4uq 165 28 LoLi LoLi NNP work_sny56posuffadh5a367n5vi4uq 165 29 − − NNP work_sny56posuffadh5a367n5vi4uq 165 30 RiRo RiRo NNP work_sny56posuffadh5a367n5vi4uq 165 31 100 100 CD work_sny56posuffadh5a367n5vi4uq 165 32 % % NN work_sny56posuffadh5a367n5vi4uq 165 33 100 100 CD work_sny56posuffadh5a367n5vi4uq 165 34 % % NN work_sny56posuffadh5a367n5vi4uq 165 35 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 165 36 30 30 CD work_sny56posuffadh5a367n5vi4uq 165 37 % % NN work_sny56posuffadh5a367n5vi4uq 165 38 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 165 39 RoRi RoRi NNP work_sny56posuffadh5a367n5vi4uq 165 40 − − NNP work_sny56posuffadh5a367n5vi4uq 165 41 RiRo RiRo NNP work_sny56posuffadh5a367n5vi4uq 165 42 42 42 CD work_sny56posuffadh5a367n5vi4uq 165 43 % % NN work_sny56posuffadh5a367n5vi4uq 165 44 29 29 CD work_sny56posuffadh5a367n5vi4uq 165 45 % % NN work_sny56posuffadh5a367n5vi4uq 165 46 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 165 47 6 6 CD work_sny56posuffadh5a367n5vi4uq 165 48 % % NN work_sny56posuffadh5a367n5vi4uq 165 49 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 165 50 LoLi LoLi NNP work_sny56posuffadh5a367n5vi4uq 165 51 − − NNP work_sny56posuffadh5a367n5vi4uq 165 52 LiLo LiLo NNP work_sny56posuffadh5a367n5vi4uq 165 53 95 95 CD work_sny56posuffadh5a367n5vi4uq 165 54 % % NN work_sny56posuffadh5a367n5vi4uq 165 55 101 101 CD work_sny56posuffadh5a367n5vi4uq 165 56 % % NN work_sny56posuffadh5a367n5vi4uq 165 57 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 165 58 30 30 CD work_sny56posuffadh5a367n5vi4uq 165 59 % % NN work_sny56posuffadh5a367n5vi4uq 165 60 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 165 61 LiLi LiLi NNP work_sny56posuffadh5a367n5vi4uq 165 62 − − NNP work_sny56posuffadh5a367n5vi4uq 165 63 RiRo RiRo NNP work_sny56posuffadh5a367n5vi4uq 165 64 nd nd NNP work_sny56posuffadh5a367n5vi4uq 165 65 4 4 CD work_sny56posuffadh5a367n5vi4uq 165 66 % % NN work_sny56posuffadh5a367n5vi4uq 165 67 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 165 68 1%)∗ 1%)∗ CD work_sny56posuffadh5a367n5vi4uq 165 69 LoLo LoLo NNP work_sny56posuffadh5a367n5vi4uq 165 70 − − NNP work_sny56posuffadh5a367n5vi4uq 165 71 RiRo RiRo NNP work_sny56posuffadh5a367n5vi4uq 165 72 46 46 CD work_sny56posuffadh5a367n5vi4uq 165 73 % % NN work_sny56posuffadh5a367n5vi4uq 165 74 43 43 CD work_sny56posuffadh5a367n5vi4uq 165 75 % % NN work_sny56posuffadh5a367n5vi4uq 165 76 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 165 77 23 23 CD work_sny56posuffadh5a367n5vi4uq 165 78 % % NN work_sny56posuffadh5a367n5vi4uq 165 79 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 165 80 Relative relative JJ work_sny56posuffadh5a367n5vi4uq 165 81 excision excision NN work_sny56posuffadh5a367n5vi4uq 165 82 activities activity NNS work_sny56posuffadh5a367n5vi4uq 165 83 were be VBD work_sny56posuffadh5a367n5vi4uq 165 84 measured measure VBN work_sny56posuffadh5a367n5vi4uq 165 85 as as IN work_sny56posuffadh5a367n5vi4uq 165 86 described describe VBN work_sny56posuffadh5a367n5vi4uq 165 87 in in IN work_sny56posuffadh5a367n5vi4uq 165 88 Figure Figure NNP work_sny56posuffadh5a367n5vi4uq 165 89 4 4 CD work_sny56posuffadh5a367n5vi4uq 165 90 . . . work_sny56posuffadh5a367n5vi4uq 166 1 The the DT work_sny56posuffadh5a367n5vi4uq 166 2 relative relative JJ work_sny56posuffadh5a367n5vi4uq 166 3 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 166 4 rates rate NNS work_sny56posuffadh5a367n5vi4uq 166 5 were be VBD work_sny56posuffadh5a367n5vi4uq 166 6 determined determine VBN work_sny56posuffadh5a367n5vi4uq 166 7 as as IN work_sny56posuffadh5a367n5vi4uq 166 8 described describe VBN work_sny56posuffadh5a367n5vi4uq 166 9 in in IN work_sny56posuffadh5a367n5vi4uq 166 10 Cui Cui NNP work_sny56posuffadh5a367n5vi4uq 166 11 et et NNP work_sny56posuffadh5a367n5vi4uq 166 12 al al NNP work_sny56posuffadh5a367n5vi4uq 166 13 . . . work_sny56posuffadh5a367n5vi4uq 167 1 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 167 2 16 16 CD work_sny56posuffadh5a367n5vi4uq 167 3 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 167 4 . . . work_sny56posuffadh5a367n5vi4uq 168 1 Transposition transposition NN work_sny56posuffadh5a367n5vi4uq 168 2 rates rate NNS work_sny56posuffadh5a367n5vi4uq 168 3 are be VBP work_sny56posuffadh5a367n5vi4uq 168 4 averages average NNS work_sny56posuffadh5a367n5vi4uq 168 5 of of IN work_sny56posuffadh5a367n5vi4uq 168 6 four four CD work_sny56posuffadh5a367n5vi4uq 168 7 to to TO work_sny56posuffadh5a367n5vi4uq 168 8 seven seven CD work_sny56posuffadh5a367n5vi4uq 168 9 independent independent JJ work_sny56posuffadh5a367n5vi4uq 168 10 transfections transfection NNS work_sny56posuffadh5a367n5vi4uq 168 11 , , , work_sny56posuffadh5a367n5vi4uq 168 12 numbers number NNS work_sny56posuffadh5a367n5vi4uq 168 13 in in IN work_sny56posuffadh5a367n5vi4uq 168 14 parentheses parenthesis NNS work_sny56posuffadh5a367n5vi4uq 168 15 show show VBP work_sny56posuffadh5a367n5vi4uq 168 16 the the DT work_sny56posuffadh5a367n5vi4uq 168 17 95 95 CD work_sny56posuffadh5a367n5vi4uq 168 18 % % NN work_sny56posuffadh5a367n5vi4uq 168 19 confidence confidence NN work_sny56posuffadh5a367n5vi4uq 168 20 interval interval NN work_sny56posuffadh5a367n5vi4uq 168 21 . . . work_sny56posuffadh5a367n5vi4uq 169 1 nd nd NNP work_sny56posuffadh5a367n5vi4uq 169 2 , , , work_sny56posuffadh5a367n5vi4uq 169 3 non non JJ work_sny56posuffadh5a367n5vi4uq 169 4 - - JJ work_sny56posuffadh5a367n5vi4uq 169 5 detectable detectable JJ work_sny56posuffadh5a367n5vi4uq 169 6 ; ; : work_sny56posuffadh5a367n5vi4uq 169 7 ∗ ∗ NNP work_sny56posuffadh5a367n5vi4uq 169 8 , , , work_sny56posuffadh5a367n5vi4uq 169 9 background background NN work_sny56posuffadh5a367n5vi4uq 169 10 level level NN work_sny56posuffadh5a367n5vi4uq 169 11 of of IN work_sny56posuffadh5a367n5vi4uq 169 12 colony colony NN work_sny56posuffadh5a367n5vi4uq 169 13 number number NN work_sny56posuffadh5a367n5vi4uq 169 14 . . . work_sny56posuffadh5a367n5vi4uq 170 1 LoLiRiRo LoLiRiRo NNP work_sny56posuffadh5a367n5vi4uq 170 2 is be VBZ work_sny56posuffadh5a367n5vi4uq 170 3 pT pT NNP work_sny56posuffadh5a367n5vi4uq 170 4 / / SYM work_sny56posuffadh5a367n5vi4uq 170 5 Hindillneo Hindillneo NNP work_sny56posuffadh5a367n5vi4uq 170 6 . . . work_sny56posuffadh5a367n5vi4uq 171 1 Figure figure NN work_sny56posuffadh5a367n5vi4uq 171 2 4 4 CD work_sny56posuffadh5a367n5vi4uq 171 3 . . . work_sny56posuffadh5a367n5vi4uq 172 1 Quantification quantification NN work_sny56posuffadh5a367n5vi4uq 172 2 of of IN work_sny56posuffadh5a367n5vi4uq 172 3 relative relative JJ work_sny56posuffadh5a367n5vi4uq 172 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 172 5 activity activity NN work_sny56posuffadh5a367n5vi4uq 172 6 in in IN work_sny56posuffadh5a367n5vi4uq 172 7 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 172 8 cell cell NN work_sny56posuffadh5a367n5vi4uq 172 9 excision excision NN work_sny56posuffadh5a367n5vi4uq 172 10 assay assay NN work_sny56posuffadh5a367n5vi4uq 172 11 . . . work_sny56posuffadh5a367n5vi4uq 173 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 173 2 levels level NNS work_sny56posuffadh5a367n5vi4uq 173 3 of of IN work_sny56posuffadh5a367n5vi4uq 173 4 four four CD work_sny56posuffadh5a367n5vi4uq 173 5 IR IR NNP work_sny56posuffadh5a367n5vi4uq 173 6 / / SYM work_sny56posuffadh5a367n5vi4uq 173 7 DR DR NNP work_sny56posuffadh5a367n5vi4uq 173 8 mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 173 9 were be VBD work_sny56posuffadh5a367n5vi4uq 173 10 measured measure VBN work_sny56posuffadh5a367n5vi4uq 173 11 relative relative JJ work_sny56posuffadh5a367n5vi4uq 173 12 to to IN work_sny56posuffadh5a367n5vi4uq 173 13 the the DT work_sny56posuffadh5a367n5vi4uq 173 14 activity activity NN work_sny56posuffadh5a367n5vi4uq 173 15 of of IN work_sny56posuffadh5a367n5vi4uq 173 16 LoLi LoLi NNP work_sny56posuffadh5a367n5vi4uq 173 17 – – : work_sny56posuffadh5a367n5vi4uq 173 18 RiRo RiRo NNP work_sny56posuffadh5a367n5vi4uq 173 19 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 173 20 the the DT work_sny56posuffadh5a367n5vi4uq 173 21 pT pT NNP work_sny56posuffadh5a367n5vi4uq 173 22 / / SYM work_sny56posuffadh5a367n5vi4uq 173 23 HindIIIneo HindIIIneo NNP work_sny56posuffadh5a367n5vi4uq 173 24 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 173 25 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 173 26 . . . work_sny56posuffadh5a367n5vi4uq 174 1 The the DT work_sny56posuffadh5a367n5vi4uq 174 2 top top JJ work_sny56posuffadh5a367n5vi4uq 174 3 gel gel NN work_sny56posuffadh5a367n5vi4uq 174 4 shows show VBZ work_sny56posuffadh5a367n5vi4uq 174 5 excision excision NN work_sny56posuffadh5a367n5vi4uq 174 6 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 174 7 products product NNS work_sny56posuffadh5a367n5vi4uq 174 8 run run VBP work_sny56posuffadh5a367n5vi4uq 174 9 on on IN work_sny56posuffadh5a367n5vi4uq 174 10 a a DT work_sny56posuffadh5a367n5vi4uq 174 11 6 6 CD work_sny56posuffadh5a367n5vi4uq 174 12 % % NN work_sny56posuffadh5a367n5vi4uq 174 13 polyacrylamide polyacrylamide NN work_sny56posuffadh5a367n5vi4uq 174 14 gel gel NN work_sny56posuffadh5a367n5vi4uq 174 15 stained stain VBN work_sny56posuffadh5a367n5vi4uq 174 16 with with IN work_sny56posuffadh5a367n5vi4uq 174 17 SYBR SYBR NNP work_sny56posuffadh5a367n5vi4uq 174 18 green green JJ work_sny56posuffadh5a367n5vi4uq 174 19 I. i. NN work_sny56posuffadh5a367n5vi4uq 175 1 The the DT work_sny56posuffadh5a367n5vi4uq 175 2 lower low JJR work_sny56posuffadh5a367n5vi4uq 175 3 gel gel NN work_sny56posuffadh5a367n5vi4uq 175 4 shows show VBZ work_sny56posuffadh5a367n5vi4uq 175 5 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 175 6 amplification amplification NN work_sny56posuffadh5a367n5vi4uq 175 7 of of IN work_sny56posuffadh5a367n5vi4uq 175 8 a a DT work_sny56posuffadh5a367n5vi4uq 175 9 segment segment NN work_sny56posuffadh5a367n5vi4uq 175 10 of of IN work_sny56posuffadh5a367n5vi4uq 175 11 the the DT work_sny56posuffadh5a367n5vi4uq 175 12 backbone backbone NN work_sny56posuffadh5a367n5vi4uq 175 13 of of IN work_sny56posuffadh5a367n5vi4uq 175 14 pT pT NNP work_sny56posuffadh5a367n5vi4uq 175 15 / / SYM work_sny56posuffadh5a367n5vi4uq 175 16 neo neo NNP work_sny56posuffadh5a367n5vi4uq 175 17 and and CC work_sny56posuffadh5a367n5vi4uq 175 18 pCMVSB pcmvsb NN work_sny56posuffadh5a367n5vi4uq 175 19 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 175 20 or or CC work_sny56posuffadh5a367n5vi4uq 175 21 pSB10- pSB10- NNP work_sny56posuffadh5a367n5vi4uq 175 22 � � , work_sny56posuffadh5a367n5vi4uq 175 23 DDE DDE NNP work_sny56posuffadh5a367n5vi4uq 175 24 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 175 25 as as IN work_sny56posuffadh5a367n5vi4uq 175 26 an an DT work_sny56posuffadh5a367n5vi4uq 175 27 input input NN work_sny56posuffadh5a367n5vi4uq 175 28 control control NN work_sny56posuffadh5a367n5vi4uq 175 29 for for IN work_sny56posuffadh5a367n5vi4uq 175 30 the the DT work_sny56posuffadh5a367n5vi4uq 175 31 total total JJ work_sny56posuffadh5a367n5vi4uq 175 32 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 175 33 in in IN work_sny56posuffadh5a367n5vi4uq 175 34 the the DT work_sny56posuffadh5a367n5vi4uq 175 35 lysate lysate NN work_sny56posuffadh5a367n5vi4uq 175 36 . . . work_sny56posuffadh5a367n5vi4uq 176 1 The the DT work_sny56posuffadh5a367n5vi4uq 176 2 relative relative JJ work_sny56posuffadh5a367n5vi4uq 176 3 excision excision NN work_sny56posuffadh5a367n5vi4uq 176 4 abundance abundance NN work_sny56posuffadh5a367n5vi4uq 176 5 was be VBD work_sny56posuffadh5a367n5vi4uq 176 6 measured measure VBN work_sny56posuffadh5a367n5vi4uq 176 7 as as IN work_sny56posuffadh5a367n5vi4uq 176 8 a a DT work_sny56posuffadh5a367n5vi4uq 176 9 ratio ratio NN work_sny56posuffadh5a367n5vi4uq 176 10 of of IN work_sny56posuffadh5a367n5vi4uq 176 11 the the DT work_sny56posuffadh5a367n5vi4uq 176 12 band band NN work_sny56posuffadh5a367n5vi4uq 176 13 intensity intensity NN work_sny56posuffadh5a367n5vi4uq 176 14 of of IN work_sny56posuffadh5a367n5vi4uq 176 15 the the DT work_sny56posuffadh5a367n5vi4uq 176 16 excision excision NN work_sny56posuffadh5a367n5vi4uq 176 17 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 176 18 products product NNS work_sny56posuffadh5a367n5vi4uq 176 19 to to IN work_sny56posuffadh5a367n5vi4uq 176 20 that that DT work_sny56posuffadh5a367n5vi4uq 176 21 of of IN work_sny56posuffadh5a367n5vi4uq 176 22 the the DT work_sny56posuffadh5a367n5vi4uq 176 23 amplification amplification NN work_sny56posuffadh5a367n5vi4uq 176 24 of of IN work_sny56posuffadh5a367n5vi4uq 176 25 the the DT work_sny56posuffadh5a367n5vi4uq 176 26 segment segment NN work_sny56posuffadh5a367n5vi4uq 176 27 on on IN work_sny56posuffadh5a367n5vi4uq 176 28 the the DT work_sny56posuffadh5a367n5vi4uq 176 29 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 176 30 backbone backbone NN work_sny56posuffadh5a367n5vi4uq 176 31 . . . work_sny56posuffadh5a367n5vi4uq 177 1 ‘ ' `` work_sny56posuffadh5a367n5vi4uq 177 2 ‘ ' `` work_sny56posuffadh5a367n5vi4uq 177 3 Rel Rel NNP work_sny56posuffadh5a367n5vi4uq 177 4 . . . work_sny56posuffadh5a367n5vi4uq 178 1 template template VB work_sny56posuffadh5a367n5vi4uq 178 2 ’ ' '' work_sny56posuffadh5a367n5vi4uq 178 3 ’ ' '' work_sny56posuffadh5a367n5vi4uq 178 4 indicates indicate VBZ work_sny56posuffadh5a367n5vi4uq 178 5 the the DT work_sny56posuffadh5a367n5vi4uq 178 6 relative relative JJ work_sny56posuffadh5a367n5vi4uq 178 7 amount amount NN work_sny56posuffadh5a367n5vi4uq 178 8 of of IN work_sny56posuffadh5a367n5vi4uq 178 9 the the DT work_sny56posuffadh5a367n5vi4uq 178 10 input input NN work_sny56posuffadh5a367n5vi4uq 178 11 lysate lysate NN work_sny56posuffadh5a367n5vi4uq 178 12 . . . work_sny56posuffadh5a367n5vi4uq 179 1 Relative relative JJ work_sny56posuffadh5a367n5vi4uq 179 2 excision excision NN work_sny56posuffadh5a367n5vi4uq 179 3 activity activity NN work_sny56posuffadh5a367n5vi4uq 179 4 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 179 5 ‘ ' `` work_sny56posuffadh5a367n5vi4uq 179 6 ‘ ' `` work_sny56posuffadh5a367n5vi4uq 179 7 Rel Rel NNP work_sny56posuffadh5a367n5vi4uq 179 8 . . . work_sny56posuffadh5a367n5vi4uq 180 1 activity activity NN work_sny56posuffadh5a367n5vi4uq 180 2 ’ ' '' work_sny56posuffadh5a367n5vi4uq 180 3 ’ ' '' work_sny56posuffadh5a367n5vi4uq 180 4 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 180 5 is be VBZ work_sny56posuffadh5a367n5vi4uq 180 6 indicated indicate VBN work_sny56posuffadh5a367n5vi4uq 180 7 as as IN work_sny56posuffadh5a367n5vi4uq 180 8 a a DT work_sny56posuffadh5a367n5vi4uq 180 9 percentage percentage NN work_sny56posuffadh5a367n5vi4uq 180 10 of of IN work_sny56posuffadh5a367n5vi4uq 180 11 control control NN work_sny56posuffadh5a367n5vi4uq 180 12 activity activity NN work_sny56posuffadh5a367n5vi4uq 180 13 using use VBG work_sny56posuffadh5a367n5vi4uq 180 14 the the DT work_sny56posuffadh5a367n5vi4uq 180 15 ratio ratio NN work_sny56posuffadh5a367n5vi4uq 180 16 for for IN work_sny56posuffadh5a367n5vi4uq 180 17 each each DT work_sny56posuffadh5a367n5vi4uq 180 18 mutation mutation NN work_sny56posuffadh5a367n5vi4uq 180 19 compared compare VBN work_sny56posuffadh5a367n5vi4uq 180 20 to to IN work_sny56posuffadh5a367n5vi4uq 180 21 a a DT work_sny56posuffadh5a367n5vi4uq 180 22 standard standard JJ work_sny56posuffadh5a367n5vi4uq 180 23 curve curve NN work_sny56posuffadh5a367n5vi4uq 180 24 derived derive VBN work_sny56posuffadh5a367n5vi4uq 180 25 from from IN work_sny56posuffadh5a367n5vi4uq 180 26 the the DT work_sny56posuffadh5a367n5vi4uq 180 27 ratio ratio NN work_sny56posuffadh5a367n5vi4uq 180 28 of of IN work_sny56posuffadh5a367n5vi4uq 180 29 the the DT work_sny56posuffadh5a367n5vi4uq 180 30 different different JJ work_sny56posuffadh5a367n5vi4uq 180 31 dilutions dilution NNS work_sny56posuffadh5a367n5vi4uq 180 32 of of IN work_sny56posuffadh5a367n5vi4uq 180 33 the the DT work_sny56posuffadh5a367n5vi4uq 180 34 original original JJ work_sny56posuffadh5a367n5vi4uq 180 35 pT pT NNP work_sny56posuffadh5a367n5vi4uq 180 36 / / SYM work_sny56posuffadh5a367n5vi4uq 180 37 HindIIIneo HindIIIneo NNP work_sny56posuffadh5a367n5vi4uq 180 38 activity activity NN work_sny56posuffadh5a367n5vi4uq 180 39 . . . work_sny56posuffadh5a367n5vi4uq 181 1 ND ND NNP work_sny56posuffadh5a367n5vi4uq 181 2 , , , work_sny56posuffadh5a367n5vi4uq 181 3 non non JJ work_sny56posuffadh5a367n5vi4uq 181 4 - - JJ work_sny56posuffadh5a367n5vi4uq 181 5 detectable detectable JJ work_sny56posuffadh5a367n5vi4uq 181 6 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 181 7 and and CC work_sny56posuffadh5a367n5vi4uq 181 8 Li Li NNP work_sny56posuffadh5a367n5vi4uq 181 9 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 181 10 showed show VBD work_sny56posuffadh5a367n5vi4uq 181 11 that that IN work_sny56posuffadh5a367n5vi4uq 181 12 there there EX work_sny56posuffadh5a367n5vi4uq 181 13 are be VBP work_sny56posuffadh5a367n5vi4uq 181 14 two two CD work_sny56posuffadh5a367n5vi4uq 181 15 discrete discrete JJ work_sny56posuffadh5a367n5vi4uq 181 16 regions region NNS work_sny56posuffadh5a367n5vi4uq 181 17 in in IN work_sny56posuffadh5a367n5vi4uq 181 18 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 181 19 that that WDT work_sny56posuffadh5a367n5vi4uq 181 20 are be VBP work_sny56posuffadh5a367n5vi4uq 181 21 different different JJ work_sny56posuffadh5a367n5vi4uq 181 22 from from IN work_sny56posuffadh5a367n5vi4uq 181 23 Li Li NNP work_sny56posuffadh5a367n5vi4uq 181 24 , , , work_sny56posuffadh5a367n5vi4uq 181 25 regions region NNS work_sny56posuffadh5a367n5vi4uq 181 26 I -PRON- PRP work_sny56posuffadh5a367n5vi4uq 181 27 and and CC work_sny56posuffadh5a367n5vi4uq 181 28 II II NNP work_sny56posuffadh5a367n5vi4uq 181 29 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 181 30 Figure Figure NNP work_sny56posuffadh5a367n5vi4uq 181 31 5A 5a RB work_sny56posuffadh5a367n5vi4uq 181 32 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 181 33 . . . work_sny56posuffadh5a367n5vi4uq 182 1 To to TO work_sny56posuffadh5a367n5vi4uq 182 2 determine determine VB work_sny56posuffadh5a367n5vi4uq 182 3 which which WDT work_sny56posuffadh5a367n5vi4uq 182 4 region region NN work_sny56posuffadh5a367n5vi4uq 182 5 is be VBZ work_sny56posuffadh5a367n5vi4uq 182 6 critical critical JJ work_sny56posuffadh5a367n5vi4uq 182 7 for for IN work_sny56posuffadh5a367n5vi4uq 182 8 excision excision NN work_sny56posuffadh5a367n5vi4uq 182 9 , , , work_sny56posuffadh5a367n5vi4uq 182 10 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 182 11 made make VBD work_sny56posuffadh5a367n5vi4uq 182 12 mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 182 13 in in IN work_sny56posuffadh5a367n5vi4uq 182 14 each each DT work_sny56posuffadh5a367n5vi4uq 182 15 and and CC work_sny56posuffadh5a367n5vi4uq 182 16 tested test VBN work_sny56posuffadh5a367n5vi4uq 182 17 whether whether IN work_sny56posuffadh5a367n5vi4uq 182 18 the the DT work_sny56posuffadh5a367n5vi4uq 182 19 excision excision NN work_sny56posuffadh5a367n5vi4uq 182 20 activity activity NN work_sny56posuffadh5a367n5vi4uq 182 21 was be VBD work_sny56posuffadh5a367n5vi4uq 182 22 impaired impair VBN work_sny56posuffadh5a367n5vi4uq 182 23 . . . work_sny56posuffadh5a367n5vi4uq 183 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 183 2 focused focus VBD work_sny56posuffadh5a367n5vi4uq 183 3 on on IN work_sny56posuffadh5a367n5vi4uq 183 4 two two CD work_sny56posuffadh5a367n5vi4uq 183 5 positions position NNS work_sny56posuffadh5a367n5vi4uq 183 6 in in IN work_sny56posuffadh5a367n5vi4uq 183 7 region region NN work_sny56posuffadh5a367n5vi4uq 183 8 I -PRON- PRP work_sny56posuffadh5a367n5vi4uq 183 9 because because IN work_sny56posuffadh5a367n5vi4uq 183 10 the the DT work_sny56posuffadh5a367n5vi4uq 183 11 model model NN work_sny56posuffadh5a367n5vi4uq 183 12 in in IN work_sny56posuffadh5a367n5vi4uq 183 13 Figure Figure NNP work_sny56posuffadh5a367n5vi4uq 183 14 1 1 CD work_sny56posuffadh5a367n5vi4uq 183 15 predicts predict VBZ work_sny56posuffadh5a367n5vi4uq 183 16 that that IN work_sny56posuffadh5a367n5vi4uq 183 17 the the DT work_sny56posuffadh5a367n5vi4uq 183 18 C C NNP work_sny56posuffadh5a367n5vi4uq 183 19 in in IN work_sny56posuffadh5a367n5vi4uq 183 20 the the DT work_sny56posuffadh5a367n5vi4uq 183 21 first first JJ work_sny56posuffadh5a367n5vi4uq 183 22 position position NN work_sny56posuffadh5a367n5vi4uq 183 23 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 183 24 C1 c1 NN work_sny56posuffadh5a367n5vi4uq 183 25 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 183 26 and and CC work_sny56posuffadh5a367n5vi4uq 183 27 the the DT work_sny56posuffadh5a367n5vi4uq 183 28 G g NN work_sny56posuffadh5a367n5vi4uq 183 29 in in IN work_sny56posuffadh5a367n5vi4uq 183 30 the the DT work_sny56posuffadh5a367n5vi4uq 183 31 third third JJ work_sny56posuffadh5a367n5vi4uq 183 32 position position NN work_sny56posuffadh5a367n5vi4uq 183 33 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 183 34 G3 g3 NN work_sny56posuffadh5a367n5vi4uq 183 35 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 183 36 at at IN work_sny56posuffadh5a367n5vi4uq 183 37 the the DT work_sny56posuffadh5a367n5vi4uq 183 38 tip tip NN work_sny56posuffadh5a367n5vi4uq 183 39 of of IN work_sny56posuffadh5a367n5vi4uq 183 40 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 183 41 and and CC work_sny56posuffadh5a367n5vi4uq 183 42 Ro Ro NNP work_sny56posuffadh5a367n5vi4uq 183 43 demarcate demarcate VB work_sny56posuffadh5a367n5vi4uq 183 44 the the DT work_sny56posuffadh5a367n5vi4uq 183 45 staggered stagger VBN work_sny56posuffadh5a367n5vi4uq 183 46 cuts cut NNS work_sny56posuffadh5a367n5vi4uq 183 47 . . . work_sny56posuffadh5a367n5vi4uq 184 1 These these DT work_sny56posuffadh5a367n5vi4uq 184 2 positions position NNS work_sny56posuffadh5a367n5vi4uq 184 3 , , , work_sny56posuffadh5a367n5vi4uq 184 4 which which WDT work_sny56posuffadh5a367n5vi4uq 184 5 are be VBP work_sny56posuffadh5a367n5vi4uq 184 6 the the DT work_sny56posuffadh5a367n5vi4uq 184 7 same same JJ work_sny56posuffadh5a367n5vi4uq 184 8 on on IN work_sny56posuffadh5a367n5vi4uq 184 9 the the DT work_sny56posuffadh5a367n5vi4uq 184 10 left left NN work_sny56posuffadh5a367n5vi4uq 184 11 and and CC work_sny56posuffadh5a367n5vi4uq 184 12 right right JJ work_sny56posuffadh5a367n5vi4uq 184 13 IRs irs NNS work_sny56posuffadh5a367n5vi4uq 184 14 , , , work_sny56posuffadh5a367n5vi4uq 184 15 should should MD work_sny56posuffadh5a367n5vi4uq 184 16 thus thus RB work_sny56posuffadh5a367n5vi4uq 184 17 be be VB work_sny56posuffadh5a367n5vi4uq 184 18 vital vital JJ work_sny56posuffadh5a367n5vi4uq 184 19 to to IN work_sny56posuffadh5a367n5vi4uq 184 20 the the DT work_sny56posuffadh5a367n5vi4uq 184 21 overall overall JJ work_sny56posuffadh5a367n5vi4uq 184 22 interaction interaction NN work_sny56posuffadh5a367n5vi4uq 184 23 between between IN work_sny56posuffadh5a367n5vi4uq 184 24 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 184 25 and and CC work_sny56posuffadh5a367n5vi4uq 184 26 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 184 27 in in IN work_sny56posuffadh5a367n5vi4uq 184 28 this this DT work_sny56posuffadh5a367n5vi4uq 184 29 region region NN work_sny56posuffadh5a367n5vi4uq 184 30 . . . work_sny56posuffadh5a367n5vi4uq 185 1 If if IN work_sny56posuffadh5a367n5vi4uq 185 2 true true JJ work_sny56posuffadh5a367n5vi4uq 185 3 , , , work_sny56posuffadh5a367n5vi4uq 185 4 changing change VBG work_sny56posuffadh5a367n5vi4uq 185 5 the the DT work_sny56posuffadh5a367n5vi4uq 185 6 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 185 7 at at IN work_sny56posuffadh5a367n5vi4uq 185 8 these these DT work_sny56posuffadh5a367n5vi4uq 185 9 two two CD work_sny56posuffadh5a367n5vi4uq 185 10 positions position NNS work_sny56posuffadh5a367n5vi4uq 185 11 should should MD work_sny56posuffadh5a367n5vi4uq 185 12 affect affect VB work_sny56posuffadh5a367n5vi4uq 185 13 this this DT work_sny56posuffadh5a367n5vi4uq 185 14 interaction interaction NN work_sny56posuffadh5a367n5vi4uq 185 15 and and CC work_sny56posuffadh5a367n5vi4uq 185 16 cause cause VB work_sny56posuffadh5a367n5vi4uq 185 17 reduced reduce VBN work_sny56posuffadh5a367n5vi4uq 185 18 excision excision NN work_sny56posuffadh5a367n5vi4uq 185 19 activity activity NN work_sny56posuffadh5a367n5vi4uq 185 20 . . . work_sny56posuffadh5a367n5vi4uq 186 1 Figure figure NN work_sny56posuffadh5a367n5vi4uq 186 2 5B 5b NN work_sny56posuffadh5a367n5vi4uq 186 3 is be VBZ work_sny56posuffadh5a367n5vi4uq 186 4 a a DT work_sny56posuffadh5a367n5vi4uq 186 5 detailed detailed JJ work_sny56posuffadh5a367n5vi4uq 186 6 analysis analysis NN work_sny56posuffadh5a367n5vi4uq 186 7 of of IN work_sny56posuffadh5a367n5vi4uq 186 8 positions position NNS work_sny56posuffadh5a367n5vi4uq 186 9 C1 c1 NN work_sny56posuffadh5a367n5vi4uq 186 10 and and CC work_sny56posuffadh5a367n5vi4uq 186 11 G3 G3 NNP work_sny56posuffadh5a367n5vi4uq 186 12 in in IN work_sny56posuffadh5a367n5vi4uq 186 13 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 186 14 and and CC work_sny56posuffadh5a367n5vi4uq 186 15 Ro Ro NNP work_sny56posuffadh5a367n5vi4uq 186 16 . . . work_sny56posuffadh5a367n5vi4uq 187 1 In in IN work_sny56posuffadh5a367n5vi4uq 187 2 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 187 3 , , , work_sny56posuffadh5a367n5vi4uq 187 4 when when WRB work_sny56posuffadh5a367n5vi4uq 187 5 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 187 6 changed change VBD work_sny56posuffadh5a367n5vi4uq 187 7 either either CC work_sny56posuffadh5a367n5vi4uq 187 8 C1 C1 NNP work_sny56posuffadh5a367n5vi4uq 187 9 or or CC work_sny56posuffadh5a367n5vi4uq 187 10 G3 g3 NN work_sny56posuffadh5a367n5vi4uq 187 11 , , , work_sny56posuffadh5a367n5vi4uq 187 12 excision excision NN work_sny56posuffadh5a367n5vi4uq 187 13 activity activity NN work_sny56posuffadh5a367n5vi4uq 187 14 was be VBD work_sny56posuffadh5a367n5vi4uq 187 15 reduced reduce VBN work_sny56posuffadh5a367n5vi4uq 187 16 to to IN work_sny56posuffadh5a367n5vi4uq 187 17 11 11 CD work_sny56posuffadh5a367n5vi4uq 187 18 and and CC work_sny56posuffadh5a367n5vi4uq 187 19 18 18 CD work_sny56posuffadh5a367n5vi4uq 187 20 % % NN work_sny56posuffadh5a367n5vi4uq 187 21 , , , work_sny56posuffadh5a367n5vi4uq 187 22 respectively respectively RB work_sny56posuffadh5a367n5vi4uq 187 23 , , , work_sny56posuffadh5a367n5vi4uq 187 24 indicating indicate VBG work_sny56posuffadh5a367n5vi4uq 187 25 that that IN work_sny56posuffadh5a367n5vi4uq 187 26 both both DT work_sny56posuffadh5a367n5vi4uq 187 27 C1 C1 NNP work_sny56posuffadh5a367n5vi4uq 187 28 and and CC work_sny56posuffadh5a367n5vi4uq 187 29 G3 G3 NNP work_sny56posuffadh5a367n5vi4uq 187 30 contribute contribute VBP work_sny56posuffadh5a367n5vi4uq 187 31 to to IN work_sny56posuffadh5a367n5vi4uq 187 32 excision excision NN work_sny56posuffadh5a367n5vi4uq 187 33 activity activity NN work_sny56posuffadh5a367n5vi4uq 187 34 . . . work_sny56posuffadh5a367n5vi4uq 188 1 The the DT work_sny56posuffadh5a367n5vi4uq 188 2 double double JJ work_sny56posuffadh5a367n5vi4uq 188 3 mutation mutation NN work_sny56posuffadh5a367n5vi4uq 188 4 at at IN work_sny56posuffadh5a367n5vi4uq 188 5 both both DT work_sny56posuffadh5a367n5vi4uq 188 6 +1 +1 NNPS work_sny56posuffadh5a367n5vi4uq 188 7 and and CC work_sny56posuffadh5a367n5vi4uq 188 8 +3 +3 HYPH work_sny56posuffadh5a367n5vi4uq 188 9 reduced reduce VBN work_sny56posuffadh5a367n5vi4uq 188 10 excision excision NN work_sny56posuffadh5a367n5vi4uq 188 11 activity activity NN work_sny56posuffadh5a367n5vi4uq 188 12 to to IN work_sny56posuffadh5a367n5vi4uq 188 13 below below IN work_sny56posuffadh5a367n5vi4uq 188 14 the the DT work_sny56posuffadh5a367n5vi4uq 188 15 limit limit NN work_sny56posuffadh5a367n5vi4uq 188 16 of of IN work_sny56posuffadh5a367n5vi4uq 188 17 detection detection NN work_sny56posuffadh5a367n5vi4uq 188 18 . . . work_sny56posuffadh5a367n5vi4uq 189 1 The the DT work_sny56posuffadh5a367n5vi4uq 189 2 same same JJ work_sny56posuffadh5a367n5vi4uq 189 3 mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 189 4 were be VBD work_sny56posuffadh5a367n5vi4uq 189 5 made make VBN work_sny56posuffadh5a367n5vi4uq 189 6 in in IN work_sny56posuffadh5a367n5vi4uq 189 7 Ro Ro NNP work_sny56posuffadh5a367n5vi4uq 189 8 and and CC work_sny56posuffadh5a367n5vi4uq 189 9 the the DT work_sny56posuffadh5a367n5vi4uq 189 10 effects effect NNS work_sny56posuffadh5a367n5vi4uq 189 11 were be VBD work_sny56posuffadh5a367n5vi4uq 189 12 similar similar JJ work_sny56posuffadh5a367n5vi4uq 189 13 , , , work_sny56posuffadh5a367n5vi4uq 189 14 indicating indicate VBG work_sny56posuffadh5a367n5vi4uq 189 15 that that IN work_sny56posuffadh5a367n5vi4uq 189 16 these these DT work_sny56posuffadh5a367n5vi4uq 189 17 positions position NNS work_sny56posuffadh5a367n5vi4uq 189 18 have have VBP work_sny56posuffadh5a367n5vi4uq 189 19 the the DT work_sny56posuffadh5a367n5vi4uq 189 20 same same JJ work_sny56posuffadh5a367n5vi4uq 189 21 functions function NNS work_sny56posuffadh5a367n5vi4uq 189 22 on on IN work_sny56posuffadh5a367n5vi4uq 189 23 both both DT work_sny56posuffadh5a367n5vi4uq 189 24 sides side NNS work_sny56posuffadh5a367n5vi4uq 189 25 . . . work_sny56posuffadh5a367n5vi4uq 190 1 These these DT work_sny56posuffadh5a367n5vi4uq 190 2 data datum NNS work_sny56posuffadh5a367n5vi4uq 190 3 suggest suggest VBP work_sny56posuffadh5a367n5vi4uq 190 4 that that IN work_sny56posuffadh5a367n5vi4uq 190 5 the the DT work_sny56posuffadh5a367n5vi4uq 190 6 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 190 7 in in IN work_sny56posuffadh5a367n5vi4uq 190 8 the the DT work_sny56posuffadh5a367n5vi4uq 190 9 first first JJ work_sny56posuffadh5a367n5vi4uq 190 10 and and CC work_sny56posuffadh5a367n5vi4uq 190 11 the the DT work_sny56posuffadh5a367n5vi4uq 190 12 third third JJ work_sny56posuffadh5a367n5vi4uq 190 13 positions position NNS work_sny56posuffadh5a367n5vi4uq 190 14 at at IN work_sny56posuffadh5a367n5vi4uq 190 15 the the DT work_sny56posuffadh5a367n5vi4uq 190 16 termini termini NN work_sny56posuffadh5a367n5vi4uq 190 17 of of IN work_sny56posuffadh5a367n5vi4uq 190 18 the the DT work_sny56posuffadh5a367n5vi4uq 190 19 outer outer JJ work_sny56posuffadh5a367n5vi4uq 190 20 DRs dr NNS work_sny56posuffadh5a367n5vi4uq 190 21 in in IN work_sny56posuffadh5a367n5vi4uq 190 22 region region NN work_sny56posuffadh5a367n5vi4uq 190 23 I -PRON- PRP work_sny56posuffadh5a367n5vi4uq 190 24 are be VBP work_sny56posuffadh5a367n5vi4uq 190 25 critical critical JJ work_sny56posuffadh5a367n5vi4uq 190 26 for for IN work_sny56posuffadh5a367n5vi4uq 190 27 excision excision NN work_sny56posuffadh5a367n5vi4uq 190 28 . . . work_sny56posuffadh5a367n5vi4uq 191 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 191 2 tested test VBD work_sny56posuffadh5a367n5vi4uq 191 3 the the DT work_sny56posuffadh5a367n5vi4uq 191 4 requirement requirement NN work_sny56posuffadh5a367n5vi4uq 191 5 for for IN work_sny56posuffadh5a367n5vi4uq 191 6 region region NN work_sny56posuffadh5a367n5vi4uq 191 7 II II NNP work_sny56posuffadh5a367n5vi4uq 191 8 by by IN work_sny56posuffadh5a367n5vi4uq 191 9 mutating mutate VBG work_sny56posuffadh5a367n5vi4uq 191 10 three three CD work_sny56posuffadh5a367n5vi4uq 191 11 out out IN work_sny56posuffadh5a367n5vi4uq 191 12 of of IN work_sny56posuffadh5a367n5vi4uq 191 13 the the DT work_sny56posuffadh5a367n5vi4uq 191 14 five five CD work_sny56posuffadh5a367n5vi4uq 191 15 terminal terminal JJ work_sny56posuffadh5a367n5vi4uq 191 16 basepairs basepair NNS work_sny56posuffadh5a367n5vi4uq 191 17 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 191 18 TTAAG ttaag NN work_sny56posuffadh5a367n5vi4uq 191 19 to to IN work_sny56posuffadh5a367n5vi4uq 191 20 GGGAG GGGAG NNP work_sny56posuffadh5a367n5vi4uq 191 21 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 191 22 . . . work_sny56posuffadh5a367n5vi4uq 192 1 Comparison Comparison NNP work_sny56posuffadh5a367n5vi4uq 192 2 of of IN work_sny56posuffadh5a367n5vi4uq 192 3 Figure Figure NNP work_sny56posuffadh5a367n5vi4uq 192 4 5C 5c NN work_sny56posuffadh5a367n5vi4uq 192 5 lanes lane VBZ work_sny56posuffadh5a367n5vi4uq 192 6 1 1 CD work_sny56posuffadh5a367n5vi4uq 192 7 and and CC work_sny56posuffadh5a367n5vi4uq 192 8 2 2 CD work_sny56posuffadh5a367n5vi4uq 192 9 indicated indicate VBD work_sny56posuffadh5a367n5vi4uq 192 10 that that IN work_sny56posuffadh5a367n5vi4uq 192 11 the the DT work_sny56posuffadh5a367n5vi4uq 192 12 exact exact JJ work_sny56posuffadh5a367n5vi4uq 192 13 sequence sequence NN work_sny56posuffadh5a367n5vi4uq 192 14 of of IN work_sny56posuffadh5a367n5vi4uq 192 15 region region NN work_sny56posuffadh5a367n5vi4uq 192 16 II II NNP work_sny56posuffadh5a367n5vi4uq 192 17 was be VBD work_sny56posuffadh5a367n5vi4uq 192 18 not not RB work_sny56posuffadh5a367n5vi4uq 192 19 critical critical JJ work_sny56posuffadh5a367n5vi4uq 192 20 for for IN work_sny56posuffadh5a367n5vi4uq 192 21 excision excision NN work_sny56posuffadh5a367n5vi4uq 192 22 because because IN work_sny56posuffadh5a367n5vi4uq 192 23 mutating mutate VBG work_sny56posuffadh5a367n5vi4uq 192 24 these these DT work_sny56posuffadh5a367n5vi4uq 192 25 three three CD work_sny56posuffadh5a367n5vi4uq 192 26 base base NN work_sny56posuffadh5a367n5vi4uq 192 27 pairs pair NNS work_sny56posuffadh5a367n5vi4uq 192 28 did do VBD work_sny56posuffadh5a367n5vi4uq 192 29 TT TT NNP work_sny56posuffadh5a367n5vi4uq 192 30 AA AA NNP work_sny56posuffadh5a367n5vi4uq 192 31 G G NNP work_sny56posuffadh5a367n5vi4uq 192 32 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 192 33 p p NN work_sny56posuffadh5a367n5vi4uq 192 34 T t NN work_sny56posuffadh5a367n5vi4uq 192 35 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 192 36 G g NN work_sny56posuffadh5a367n5vi4uq 192 37 G g NN work_sny56posuffadh5a367n5vi4uq 192 38 G g NN work_sny56posuffadh5a367n5vi4uq 192 39 AG AG NNP work_sny56posuffadh5a367n5vi4uq 192 40 M m NN work_sny56posuffadh5a367n5vi4uq 192 41 1 1 CD work_sny56posuffadh5a367n5vi4uq 192 42 2 2 CD work_sny56posuffadh5a367n5vi4uq 192 43 C c NN work_sny56posuffadh5a367n5vi4uq 192 44 B B NNP work_sny56posuffadh5a367n5vi4uq 192 45 A A NNP work_sny56posuffadh5a367n5vi4uq 192 46 Figure figure NN work_sny56posuffadh5a367n5vi4uq 192 47 5 5 CD work_sny56posuffadh5a367n5vi4uq 192 48 . . . work_sny56posuffadh5a367n5vi4uq 193 1 Terminal terminal JJ work_sny56posuffadh5a367n5vi4uq 193 2 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 193 3 in in IN work_sny56posuffadh5a367n5vi4uq 193 4 the the DT work_sny56posuffadh5a367n5vi4uq 193 5 outer outer JJ work_sny56posuffadh5a367n5vi4uq 193 6 DRs dr NNS work_sny56posuffadh5a367n5vi4uq 193 7 are be VBP work_sny56posuffadh5a367n5vi4uq 193 8 important important JJ work_sny56posuffadh5a367n5vi4uq 193 9 for for IN work_sny56posuffadh5a367n5vi4uq 193 10 excision excision NN work_sny56posuffadh5a367n5vi4uq 193 11 . . . work_sny56posuffadh5a367n5vi4uq 194 1 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 194 2 A a DT work_sny56posuffadh5a367n5vi4uq 194 3 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 194 4 Sequence sequence NN work_sny56posuffadh5a367n5vi4uq 194 5 comparison comparison NN work_sny56posuffadh5a367n5vi4uq 194 6 of of IN work_sny56posuffadh5a367n5vi4uq 194 7 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 194 8 and and CC work_sny56posuffadh5a367n5vi4uq 194 9 Li Li NNP work_sny56posuffadh5a367n5vi4uq 194 10 . . . work_sny56posuffadh5a367n5vi4uq 195 1 Mutated mutate VBN work_sny56posuffadh5a367n5vi4uq 195 2 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 195 3 in in IN work_sny56posuffadh5a367n5vi4uq 195 4 regions region NNS work_sny56posuffadh5a367n5vi4uq 195 5 I -PRON- PRP work_sny56posuffadh5a367n5vi4uq 195 6 and and CC work_sny56posuffadh5a367n5vi4uq 195 7 II II NNP work_sny56posuffadh5a367n5vi4uq 195 8 are be VBP work_sny56posuffadh5a367n5vi4uq 195 9 underlined underline VBN work_sny56posuffadh5a367n5vi4uq 195 10 . . . work_sny56posuffadh5a367n5vi4uq 196 1 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 196 2 B b NN work_sny56posuffadh5a367n5vi4uq 196 3 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 196 4 Mutations Mutations NNPS work_sny56posuffadh5a367n5vi4uq 196 5 in in IN work_sny56posuffadh5a367n5vi4uq 196 6 region region NN work_sny56posuffadh5a367n5vi4uq 196 7 I -PRON- PRP work_sny56posuffadh5a367n5vi4uq 196 8 at at IN work_sny56posuffadh5a367n5vi4uq 196 9 the the DT work_sny56posuffadh5a367n5vi4uq 196 10 first first JJ work_sny56posuffadh5a367n5vi4uq 196 11 and and CC work_sny56posuffadh5a367n5vi4uq 196 12 third third JJ work_sny56posuffadh5a367n5vi4uq 196 13 positions position NNS work_sny56posuffadh5a367n5vi4uq 196 14 at at IN work_sny56posuffadh5a367n5vi4uq 196 15 the the DT work_sny56posuffadh5a367n5vi4uq 196 16 terminus terminus NN work_sny56posuffadh5a367n5vi4uq 196 17 of of IN work_sny56posuffadh5a367n5vi4uq 196 18 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 196 19 are be VBP work_sny56posuffadh5a367n5vi4uq 196 20 underlined underline VBN work_sny56posuffadh5a367n5vi4uq 196 21 . . . work_sny56posuffadh5a367n5vi4uq 197 1 Lo(CAG)Li Lo(CAG)Li NNS work_sny56posuffadh5a367n5vi4uq 197 2 – – : work_sny56posuffadh5a367n5vi4uq 197 3 Ri(CTG)Ro Ri(CTG)Ro NNP work_sny56posuffadh5a367n5vi4uq 197 4 indicate indicate VBP work_sny56posuffadh5a367n5vi4uq 197 5 the the DT work_sny56posuffadh5a367n5vi4uq 197 6 pT pT NNP work_sny56posuffadh5a367n5vi4uq 197 7 / / SYM work_sny56posuffadh5a367n5vi4uq 197 8 HindIIIneo HindIIIneo NNP work_sny56posuffadh5a367n5vi4uq 197 9 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 197 10 . . . work_sny56posuffadh5a367n5vi4uq 198 1 Quantification quantification NN work_sny56posuffadh5a367n5vi4uq 198 2 of of IN work_sny56posuffadh5a367n5vi4uq 198 3 the the DT work_sny56posuffadh5a367n5vi4uq 198 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 198 5 activity activity NN work_sny56posuffadh5a367n5vi4uq 198 6 is be VBZ work_sny56posuffadh5a367n5vi4uq 198 7 as as RB work_sny56posuffadh5a367n5vi4uq 198 8 described describe VBN work_sny56posuffadh5a367n5vi4uq 198 9 in in IN work_sny56posuffadh5a367n5vi4uq 198 10 Figure Figure NNP work_sny56posuffadh5a367n5vi4uq 198 11 4 4 CD work_sny56posuffadh5a367n5vi4uq 198 12 . . . work_sny56posuffadh5a367n5vi4uq 199 1 ND ND NNP work_sny56posuffadh5a367n5vi4uq 199 2 , , , work_sny56posuffadh5a367n5vi4uq 199 3 non non JJ work_sny56posuffadh5a367n5vi4uq 199 4 - - JJ work_sny56posuffadh5a367n5vi4uq 199 5 detectable detectable JJ work_sny56posuffadh5a367n5vi4uq 199 6 . . . work_sny56posuffadh5a367n5vi4uq 200 1 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 200 2 C C NNP work_sny56posuffadh5a367n5vi4uq 200 3 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 200 4 Mutations Mutations NNPS work_sny56posuffadh5a367n5vi4uq 200 5 in in IN work_sny56posuffadh5a367n5vi4uq 200 6 region region NN work_sny56posuffadh5a367n5vi4uq 200 7 II ii CD work_sny56posuffadh5a367n5vi4uq 200 8 wherein wherein WRB work_sny56posuffadh5a367n5vi4uq 200 9 three three CD work_sny56posuffadh5a367n5vi4uq 200 10 out out IN work_sny56posuffadh5a367n5vi4uq 200 11 of of IN work_sny56posuffadh5a367n5vi4uq 200 12 the the DT work_sny56posuffadh5a367n5vi4uq 200 13 five five CD work_sny56posuffadh5a367n5vi4uq 200 14 terminal terminal JJ work_sny56posuffadh5a367n5vi4uq 200 15 base base NN work_sny56posuffadh5a367n5vi4uq 200 16 pairs pair NNS work_sny56posuffadh5a367n5vi4uq 200 17 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 200 18 TTAAG ttaag NN work_sny56posuffadh5a367n5vi4uq 200 19 to to IN work_sny56posuffadh5a367n5vi4uq 200 20 GGGAG GGGAG NNP work_sny56posuffadh5a367n5vi4uq 200 21 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 200 22 were be VBD work_sny56posuffadh5a367n5vi4uq 200 23 changed change VBN work_sny56posuffadh5a367n5vi4uq 200 24 Copyright copyright NN work_sny56posuffadh5a367n5vi4uq 200 25   CD work_sny56posuffadh5a367n5vi4uq 200 26 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 200 27 John John NNP work_sny56posuffadh5a367n5vi4uq 200 28 Wiley Wiley NNP work_sny56posuffadh5a367n5vi4uq 200 29 & & CC work_sny56posuffadh5a367n5vi4uq 200 30 Sons Sons NNPS work_sny56posuffadh5a367n5vi4uq 200 31 , , , work_sny56posuffadh5a367n5vi4uq 200 32 Ltd. Ltd. NNP work_sny56posuffadh5a367n5vi4uq 200 33 J J NNP work_sny56posuffadh5a367n5vi4uq 200 34 Gene Gene NNP work_sny56posuffadh5a367n5vi4uq 200 35 Med Med NNP work_sny56posuffadh5a367n5vi4uq 200 36 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 200 37 ; ; : work_sny56posuffadh5a367n5vi4uq 200 38 6 6 CD work_sny56posuffadh5a367n5vi4uq 200 39 : : : work_sny56posuffadh5a367n5vi4uq 200 40 574 574 CD work_sny56posuffadh5a367n5vi4uq 200 41 – – : work_sny56posuffadh5a367n5vi4uq 200 42 583 583 CD work_sny56posuffadh5a367n5vi4uq 200 43 . . . work_sny56posuffadh5a367n5vi4uq 201 1 580 580 CD work_sny56posuffadh5a367n5vi4uq 201 2 G. G. NNP work_sny56posuffadh5a367n5vi4uq 201 3 Liu Liu NNP work_sny56posuffadh5a367n5vi4uq 201 4 et et NNP work_sny56posuffadh5a367n5vi4uq 201 5 al al NNP work_sny56posuffadh5a367n5vi4uq 201 6 . . . work_sny56posuffadh5a367n5vi4uq 202 1 not not RB work_sny56posuffadh5a367n5vi4uq 202 2 reduce reduce VB work_sny56posuffadh5a367n5vi4uq 202 3 excision excision NN work_sny56posuffadh5a367n5vi4uq 202 4 activity activity NN work_sny56posuffadh5a367n5vi4uq 202 5 . . . work_sny56posuffadh5a367n5vi4uq 203 1 Instead instead RB work_sny56posuffadh5a367n5vi4uq 203 2 , , , work_sny56posuffadh5a367n5vi4uq 203 3 the the DT work_sny56posuffadh5a367n5vi4uq 203 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 203 5 activity activity NN work_sny56posuffadh5a367n5vi4uq 203 6 seems seem VBZ work_sny56posuffadh5a367n5vi4uq 203 7 to to TO work_sny56posuffadh5a367n5vi4uq 203 8 have have VB work_sny56posuffadh5a367n5vi4uq 203 9 been be VBN work_sny56posuffadh5a367n5vi4uq 203 10 increased increase VBN work_sny56posuffadh5a367n5vi4uq 203 11 . . . work_sny56posuffadh5a367n5vi4uq 204 1 The the DT work_sny56posuffadh5a367n5vi4uq 204 2 band band NN work_sny56posuffadh5a367n5vi4uq 204 3 intensity intensity NN work_sny56posuffadh5a367n5vi4uq 204 4 for for IN work_sny56posuffadh5a367n5vi4uq 204 5 the the DT work_sny56posuffadh5a367n5vi4uq 204 6 GGGAG GGGAG NNP work_sny56posuffadh5a367n5vi4uq 204 7 mutation mutation NN work_sny56posuffadh5a367n5vi4uq 204 8 construct construct NN work_sny56posuffadh5a367n5vi4uq 204 9 was be VBD work_sny56posuffadh5a367n5vi4uq 204 10 eight eight CD work_sny56posuffadh5a367n5vi4uq 204 11 times time NNS work_sny56posuffadh5a367n5vi4uq 204 12 more more RBR work_sny56posuffadh5a367n5vi4uq 204 13 intense intense JJ work_sny56posuffadh5a367n5vi4uq 204 14 than than IN work_sny56posuffadh5a367n5vi4uq 204 15 that that DT work_sny56posuffadh5a367n5vi4uq 204 16 of of IN work_sny56posuffadh5a367n5vi4uq 204 17 the the DT work_sny56posuffadh5a367n5vi4uq 204 18 pT pT NNP work_sny56posuffadh5a367n5vi4uq 204 19 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 204 20 TTAAG TTAAG NNP work_sny56posuffadh5a367n5vi4uq 204 21 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 204 22 , , , work_sny56posuffadh5a367n5vi4uq 204 23 which which WDT work_sny56posuffadh5a367n5vi4uq 204 24 exceeded exceed VBD work_sny56posuffadh5a367n5vi4uq 204 25 the the DT work_sny56posuffadh5a367n5vi4uq 204 26 limit limit NN work_sny56posuffadh5a367n5vi4uq 204 27 of of IN work_sny56posuffadh5a367n5vi4uq 204 28 our -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 204 29 detection detection NN work_sny56posuffadh5a367n5vi4uq 204 30 method method NN work_sny56posuffadh5a367n5vi4uq 204 31 . . . work_sny56posuffadh5a367n5vi4uq 205 1 This this DT work_sny56posuffadh5a367n5vi4uq 205 2 trend trend NN work_sny56posuffadh5a367n5vi4uq 205 3 persisted persist VBD work_sny56posuffadh5a367n5vi4uq 205 4 in in IN work_sny56posuffadh5a367n5vi4uq 205 5 repeated repeat VBN work_sny56posuffadh5a367n5vi4uq 205 6 experiments experiment NNS work_sny56posuffadh5a367n5vi4uq 205 7 , , , work_sny56posuffadh5a367n5vi4uq 205 8 leading lead VBG work_sny56posuffadh5a367n5vi4uq 205 9 us -PRON- PRP work_sny56posuffadh5a367n5vi4uq 205 10 to to TO work_sny56posuffadh5a367n5vi4uq 205 11 incorporate incorporate VB work_sny56posuffadh5a367n5vi4uq 205 12 these these DT work_sny56posuffadh5a367n5vi4uq 205 13 mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 205 14 in in IN work_sny56posuffadh5a367n5vi4uq 205 15 the the DT work_sny56posuffadh5a367n5vi4uq 205 16 next next JJ work_sny56posuffadh5a367n5vi4uq 205 17 generation generation NN work_sny56posuffadh5a367n5vi4uq 205 18 of of IN work_sny56posuffadh5a367n5vi4uq 205 19 improved improved JJ work_sny56posuffadh5a367n5vi4uq 205 20 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 205 21 DNA DNA NNP work_sny56posuffadh5a367n5vi4uq 205 22 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 205 23 unpublished unpublished JJ work_sny56posuffadh5a367n5vi4uq 205 24 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 205 25 . . . work_sny56posuffadh5a367n5vi4uq 206 1 TA TA NNP work_sny56posuffadh5a367n5vi4uq 206 2 - - HYPH work_sny56posuffadh5a367n5vi4uq 206 3 dinucleotides dinucleotide NNS work_sny56posuffadh5a367n5vi4uq 206 4 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 206 5 the the DT work_sny56posuffadh5a367n5vi4uq 206 6 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 206 7 affect affect VB work_sny56posuffadh5a367n5vi4uq 206 8 excision excision NN work_sny56posuffadh5a367n5vi4uq 206 9 The the DT work_sny56posuffadh5a367n5vi4uq 206 10 TA TA NNP work_sny56posuffadh5a367n5vi4uq 206 11 - - HYPH work_sny56posuffadh5a367n5vi4uq 206 12 dinucleotides dinucleotide NNS work_sny56posuffadh5a367n5vi4uq 206 13 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 206 14 the the DT work_sny56posuffadh5a367n5vi4uq 206 15 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 206 16 are be VBP work_sny56posuffadh5a367n5vi4uq 206 17 of of IN work_sny56posuffadh5a367n5vi4uq 206 18 special special JJ work_sny56posuffadh5a367n5vi4uq 206 19 interest interest NN work_sny56posuffadh5a367n5vi4uq 206 20 . . . work_sny56posuffadh5a367n5vi4uq 207 1 SB SB NNP work_sny56posuffadh5a367n5vi4uq 207 2 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 207 3 insert insert VBP work_sny56posuffadh5a367n5vi4uq 207 4 only only RB work_sny56posuffadh5a367n5vi4uq 207 5 into into IN work_sny56posuffadh5a367n5vi4uq 207 6 TA- TA- NNP work_sny56posuffadh5a367n5vi4uq 207 7 dinucleotides dinucleotide NNS work_sny56posuffadh5a367n5vi4uq 207 8 , , , work_sny56posuffadh5a367n5vi4uq 207 9 where where WRB work_sny56posuffadh5a367n5vi4uq 207 10 a a DT work_sny56posuffadh5a367n5vi4uq 207 11 target target NN work_sny56posuffadh5a367n5vi4uq 207 12 - - HYPH work_sny56posuffadh5a367n5vi4uq 207 13 site site NN work_sny56posuffadh5a367n5vi4uq 207 14 duplication duplication NN work_sny56posuffadh5a367n5vi4uq 207 15 event event NN work_sny56posuffadh5a367n5vi4uq 207 16 leads lead VBZ work_sny56posuffadh5a367n5vi4uq 207 17 to to IN work_sny56posuffadh5a367n5vi4uq 207 18 TA TA NNP work_sny56posuffadh5a367n5vi4uq 207 19 flanks flank NNS work_sny56posuffadh5a367n5vi4uq 207 20 on on IN work_sny56posuffadh5a367n5vi4uq 207 21 both both DT work_sny56posuffadh5a367n5vi4uq 207 22 sides side NNS work_sny56posuffadh5a367n5vi4uq 207 23 of of IN work_sny56posuffadh5a367n5vi4uq 207 24 the the DT work_sny56posuffadh5a367n5vi4uq 207 25 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 207 26 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 207 27 1 1 CD work_sny56posuffadh5a367n5vi4uq 207 28 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 207 29 . . . work_sny56posuffadh5a367n5vi4uq 208 1 This this DT work_sny56posuffadh5a367n5vi4uq 208 2 raised raise VBD work_sny56posuffadh5a367n5vi4uq 208 3 the the DT work_sny56posuffadh5a367n5vi4uq 208 4 question question NN work_sny56posuffadh5a367n5vi4uq 208 5 of of IN work_sny56posuffadh5a367n5vi4uq 208 6 whether whether IN work_sny56posuffadh5a367n5vi4uq 208 7 there there EX work_sny56posuffadh5a367n5vi4uq 208 8 is be VBZ work_sny56posuffadh5a367n5vi4uq 208 9 a a DT work_sny56posuffadh5a367n5vi4uq 208 10 functional functional JJ work_sny56posuffadh5a367n5vi4uq 208 11 requirement requirement NN work_sny56posuffadh5a367n5vi4uq 208 12 for for IN work_sny56posuffadh5a367n5vi4uq 208 13 TA TA NNP work_sny56posuffadh5a367n5vi4uq 208 14 in in IN work_sny56posuffadh5a367n5vi4uq 208 15 excision excision NN work_sny56posuffadh5a367n5vi4uq 208 16 . . . work_sny56posuffadh5a367n5vi4uq 209 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 209 2 examined examine VBD work_sny56posuffadh5a367n5vi4uq 209 3 this this DT work_sny56posuffadh5a367n5vi4uq 209 4 question question NN work_sny56posuffadh5a367n5vi4uq 209 5 by by IN work_sny56posuffadh5a367n5vi4uq 209 6 replacing replace VBG work_sny56posuffadh5a367n5vi4uq 209 7 the the DT work_sny56posuffadh5a367n5vi4uq 209 8 TA TA NNP work_sny56posuffadh5a367n5vi4uq 209 9 - - HYPH work_sny56posuffadh5a367n5vi4uq 209 10 dinucleotides dinucleotide NNS work_sny56posuffadh5a367n5vi4uq 209 11 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 209 12 the the DT work_sny56posuffadh5a367n5vi4uq 209 13 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 209 14 . . . work_sny56posuffadh5a367n5vi4uq 210 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 210 2 found find VBD work_sny56posuffadh5a367n5vi4uq 210 3 that that IN work_sny56posuffadh5a367n5vi4uq 210 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 210 5 can can MD work_sny56posuffadh5a367n5vi4uq 210 6 still still RB work_sny56posuffadh5a367n5vi4uq 210 7 occur occur VB work_sny56posuffadh5a367n5vi4uq 210 8 when when WRB work_sny56posuffadh5a367n5vi4uq 210 9 only only RB work_sny56posuffadh5a367n5vi4uq 210 10 one one CD work_sny56posuffadh5a367n5vi4uq 210 11 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 210 12 TA TA NNP work_sny56posuffadh5a367n5vi4uq 210 13 is be VBZ work_sny56posuffadh5a367n5vi4uq 210 14 present present JJ work_sny56posuffadh5a367n5vi4uq 210 15 , , , work_sny56posuffadh5a367n5vi4uq 210 16 although although IN work_sny56posuffadh5a367n5vi4uq 210 17 activity activity NN work_sny56posuffadh5a367n5vi4uq 210 18 is be VBZ work_sny56posuffadh5a367n5vi4uq 210 19 dramatically dramatically RB work_sny56posuffadh5a367n5vi4uq 210 20 reduced reduce VBN work_sny56posuffadh5a367n5vi4uq 210 21 . . . work_sny56posuffadh5a367n5vi4uq 211 1 When when WRB work_sny56posuffadh5a367n5vi4uq 211 2 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 211 3 replaced replace VBD work_sny56posuffadh5a367n5vi4uq 211 4 both both DT work_sny56posuffadh5a367n5vi4uq 211 5 TAs ta NNS work_sny56posuffadh5a367n5vi4uq 211 6 , , , work_sny56posuffadh5a367n5vi4uq 211 7 the the DT work_sny56posuffadh5a367n5vi4uq 211 8 excision excision NN work_sny56posuffadh5a367n5vi4uq 211 9 rate rate NN work_sny56posuffadh5a367n5vi4uq 211 10 was be VBD work_sny56posuffadh5a367n5vi4uq 211 11 reduced reduce VBN work_sny56posuffadh5a367n5vi4uq 211 12 to to IN work_sny56posuffadh5a367n5vi4uq 211 13 below below IN work_sny56posuffadh5a367n5vi4uq 211 14 the the DT work_sny56posuffadh5a367n5vi4uq 211 15 limit limit NN work_sny56posuffadh5a367n5vi4uq 211 16 of of IN work_sny56posuffadh5a367n5vi4uq 211 17 detection detection NN work_sny56posuffadh5a367n5vi4uq 211 18 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 211 19 Figure figure NN work_sny56posuffadh5a367n5vi4uq 211 20 6A 6a NN work_sny56posuffadh5a367n5vi4uq 211 21 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 211 22 . . . work_sny56posuffadh5a367n5vi4uq 212 1 These these DT work_sny56posuffadh5a367n5vi4uq 212 2 results result NNS work_sny56posuffadh5a367n5vi4uq 212 3 show show VBP work_sny56posuffadh5a367n5vi4uq 212 4 that that IN work_sny56posuffadh5a367n5vi4uq 212 5 the the DT work_sny56posuffadh5a367n5vi4uq 212 6 TAs ta NNS work_sny56posuffadh5a367n5vi4uq 212 7 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 212 8 the the DT work_sny56posuffadh5a367n5vi4uq 212 9 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 212 10 are be VBP work_sny56posuffadh5a367n5vi4uq 212 11 strongly strongly RB work_sny56posuffadh5a367n5vi4uq 212 12 involved involve VBN work_sny56posuffadh5a367n5vi4uq 212 13 in in IN work_sny56posuffadh5a367n5vi4uq 212 14 excision excision NN work_sny56posuffadh5a367n5vi4uq 212 15 and and CC work_sny56posuffadh5a367n5vi4uq 212 16 confirm confirm VB work_sny56posuffadh5a367n5vi4uq 212 17 our -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 212 18 earlier early JJR work_sny56posuffadh5a367n5vi4uq 212 19 finding finding NN work_sny56posuffadh5a367n5vi4uq 212 20 of of IN work_sny56posuffadh5a367n5vi4uq 212 21 their -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 212 22 importance importance NN work_sny56posuffadh5a367n5vi4uq 212 23 in in IN work_sny56posuffadh5a367n5vi4uq 212 24 the the DT work_sny56posuffadh5a367n5vi4uq 212 25 overall overall JJ work_sny56posuffadh5a367n5vi4uq 212 26 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 212 27 process process NN work_sny56posuffadh5a367n5vi4uq 212 28 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 212 29 16 16 CD work_sny56posuffadh5a367n5vi4uq 212 30 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 212 31 . . . work_sny56posuffadh5a367n5vi4uq 213 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 213 2 were be VBD work_sny56posuffadh5a367n5vi4uq 213 3 able able JJ work_sny56posuffadh5a367n5vi4uq 213 4 to to TO work_sny56posuffadh5a367n5vi4uq 213 5 acquire acquire VB work_sny56posuffadh5a367n5vi4uq 213 6 complete complete JJ work_sny56posuffadh5a367n5vi4uq 213 7 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 213 8 from from IN work_sny56posuffadh5a367n5vi4uq 213 9 the the DT work_sny56posuffadh5a367n5vi4uq 213 10 two two CD work_sny56posuffadh5a367n5vi4uq 213 11 mutation mutation NN work_sny56posuffadh5a367n5vi4uq 213 12 constructs construct NNS work_sny56posuffadh5a367n5vi4uq 213 13 lacking lack VBG work_sny56posuffadh5a367n5vi4uq 213 14 TA TA NNP work_sny56posuffadh5a367n5vi4uq 213 15 on on IN work_sny56posuffadh5a367n5vi4uq 213 16 one one CD work_sny56posuffadh5a367n5vi4uq 213 17 side side NN work_sny56posuffadh5a367n5vi4uq 213 18 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 213 19 Figure figure VB work_sny56posuffadh5a367n5vi4uq 213 20 6B 6b NN work_sny56posuffadh5a367n5vi4uq 213 21 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 213 22 . . . work_sny56posuffadh5a367n5vi4uq 214 1 The the DT work_sny56posuffadh5a367n5vi4uq 214 2 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 214 3 showed show VBD work_sny56posuffadh5a367n5vi4uq 214 4 that that IN work_sny56posuffadh5a367n5vi4uq 214 5 when when WRB work_sny56posuffadh5a367n5vi4uq 214 6 GG GG NNP work_sny56posuffadh5a367n5vi4uq 214 7 replaced replace VBD work_sny56posuffadh5a367n5vi4uq 214 8 the the DT work_sny56posuffadh5a367n5vi4uq 214 9 TA TA NNP work_sny56posuffadh5a367n5vi4uq 214 10 outside outside IN work_sny56posuffadh5a367n5vi4uq 214 11 the the DT work_sny56posuffadh5a367n5vi4uq 214 12 outer outer JJ work_sny56posuffadh5a367n5vi4uq 214 13 DR DR NNP work_sny56posuffadh5a367n5vi4uq 214 14 , , , work_sny56posuffadh5a367n5vi4uq 214 15 the the DT work_sny56posuffadh5a367n5vi4uq 214 16 substitution substitution NN work_sny56posuffadh5a367n5vi4uq 214 17 remained remain VBD work_sny56posuffadh5a367n5vi4uq 214 18 in in IN work_sny56posuffadh5a367n5vi4uq 214 19 the the DT work_sny56posuffadh5a367n5vi4uq 214 20 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 214 21 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 214 22 underlined underline VBN work_sny56posuffadh5a367n5vi4uq 214 23 GGs gg NNS work_sny56posuffadh5a367n5vi4uq 214 24 in in IN work_sny56posuffadh5a367n5vi4uq 214 25 Figure Figure NNP work_sny56posuffadh5a367n5vi4uq 214 26 6B 6b NN work_sny56posuffadh5a367n5vi4uq 214 27 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 214 28 . . . work_sny56posuffadh5a367n5vi4uq 215 1 Use use NN work_sny56posuffadh5a367n5vi4uq 215 2 of of IN work_sny56posuffadh5a367n5vi4uq 215 3 the the DT work_sny56posuffadh5a367n5vi4uq 215 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 215 5 assay assay NN work_sny56posuffadh5a367n5vi4uq 215 6 to to TO work_sny56posuffadh5a367n5vi4uq 215 7 evaluate evaluate VB work_sny56posuffadh5a367n5vi4uq 215 8 SB SB NNP work_sny56posuffadh5a367n5vi4uq 215 9 activity activity NN work_sny56posuffadh5a367n5vi4uq 215 10 in in IN work_sny56posuffadh5a367n5vi4uq 215 11 adult adult NN work_sny56posuffadh5a367n5vi4uq 215 12 animals animal VBZ work_sny56posuffadh5a367n5vi4uq 215 13 The the DT work_sny56posuffadh5a367n5vi4uq 215 14 SB SB NNP work_sny56posuffadh5a367n5vi4uq 215 15 transposon transposon NN work_sny56posuffadh5a367n5vi4uq 215 16 system system NN work_sny56posuffadh5a367n5vi4uq 215 17 has have VBZ work_sny56posuffadh5a367n5vi4uq 215 18 been be VBN work_sny56posuffadh5a367n5vi4uq 215 19 used use VBN work_sny56posuffadh5a367n5vi4uq 215 20 for for IN work_sny56posuffadh5a367n5vi4uq 215 21 gene gene NN work_sny56posuffadh5a367n5vi4uq 215 22 transfer transfer NN work_sny56posuffadh5a367n5vi4uq 215 23 into into IN work_sny56posuffadh5a367n5vi4uq 215 24 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 215 25 as as IN work_sny56posuffadh5a367n5vi4uq 215 26 a a DT work_sny56posuffadh5a367n5vi4uq 215 27 model model NN work_sny56posuffadh5a367n5vi4uq 215 28 for for IN work_sny56posuffadh5a367n5vi4uq 215 29 use use NN work_sny56posuffadh5a367n5vi4uq 215 30 in in IN work_sny56posuffadh5a367n5vi4uq 215 31 human human JJ work_sny56posuffadh5a367n5vi4uq 215 32 gene gene NN work_sny56posuffadh5a367n5vi4uq 215 33 therapy therapy NN work_sny56posuffadh5a367n5vi4uq 215 34 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 215 35 10 10 CD work_sny56posuffadh5a367n5vi4uq 215 36 – – : work_sny56posuffadh5a367n5vi4uq 215 37 12 12 CD work_sny56posuffadh5a367n5vi4uq 215 38 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 215 39 . . . work_sny56posuffadh5a367n5vi4uq 216 1 However however RB work_sny56posuffadh5a367n5vi4uq 216 2 , , , work_sny56posuffadh5a367n5vi4uq 216 3 evaluating evaluate VBG work_sny56posuffadh5a367n5vi4uq 216 4 the the DT work_sny56posuffadh5a367n5vi4uq 216 5 efficacy efficacy NN work_sny56posuffadh5a367n5vi4uq 216 6 of of IN work_sny56posuffadh5a367n5vi4uq 216 7 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 216 8 is be VBZ work_sny56posuffadh5a367n5vi4uq 216 9 difficult difficult JJ work_sny56posuffadh5a367n5vi4uq 216 10 because because IN work_sny56posuffadh5a367n5vi4uq 216 11 delivery delivery NN work_sny56posuffadh5a367n5vi4uq 216 12 of of IN work_sny56posuffadh5a367n5vi4uq 216 13 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 216 14 to to IN work_sny56posuffadh5a367n5vi4uq 216 15 many many JJ work_sny56posuffadh5a367n5vi4uq 216 16 different different JJ work_sny56posuffadh5a367n5vi4uq 216 17 cells cell NNS work_sny56posuffadh5a367n5vi4uq 216 18 of of IN work_sny56posuffadh5a367n5vi4uq 216 19 an an DT work_sny56posuffadh5a367n5vi4uq 216 20 organ organ NN work_sny56posuffadh5a367n5vi4uq 216 21 results result NNS work_sny56posuffadh5a367n5vi4uq 216 22 in in IN work_sny56posuffadh5a367n5vi4uq 216 23 integration integration NN work_sny56posuffadh5a367n5vi4uq 216 24 events event NNS work_sny56posuffadh5a367n5vi4uq 216 25 in in IN work_sny56posuffadh5a367n5vi4uq 216 26 different different JJ work_sny56posuffadh5a367n5vi4uq 216 27 sites site NNS work_sny56posuffadh5a367n5vi4uq 216 28 of of IN work_sny56posuffadh5a367n5vi4uq 216 29 various various JJ work_sny56posuffadh5a367n5vi4uq 216 30 chromosomes chromosome NNS work_sny56posuffadh5a367n5vi4uq 216 31 . . . work_sny56posuffadh5a367n5vi4uq 217 1 Hence hence RB work_sny56posuffadh5a367n5vi4uq 217 2 , , , work_sny56posuffadh5a367n5vi4uq 217 3 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 217 4 examined examine VBD work_sny56posuffadh5a367n5vi4uq 217 5 whether whether IN work_sny56posuffadh5a367n5vi4uq 217 6 the the DT work_sny56posuffadh5a367n5vi4uq 217 7 excision excision NN work_sny56posuffadh5a367n5vi4uq 217 8 assay assay NN work_sny56posuffadh5a367n5vi4uq 217 9 could could MD work_sny56posuffadh5a367n5vi4uq 217 10 be be VB work_sny56posuffadh5a367n5vi4uq 217 11 used use VBN work_sny56posuffadh5a367n5vi4uq 217 12 for for IN work_sny56posuffadh5a367n5vi4uq 217 13 this this DT work_sny56posuffadh5a367n5vi4uq 217 14 purpose purpose NN work_sny56posuffadh5a367n5vi4uq 217 15 . . . work_sny56posuffadh5a367n5vi4uq 218 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 218 2 used use VBD work_sny56posuffadh5a367n5vi4uq 218 3 the the DT work_sny56posuffadh5a367n5vi4uq 218 4 hydrodynamic hydrodynamic JJ work_sny56posuffadh5a367n5vi4uq 218 5 delivery delivery NN work_sny56posuffadh5a367n5vi4uq 218 6 method method NN work_sny56posuffadh5a367n5vi4uq 218 7 of of IN work_sny56posuffadh5a367n5vi4uq 218 8 Liu Liu NNP work_sny56posuffadh5a367n5vi4uq 218 9 et et NNP work_sny56posuffadh5a367n5vi4uq 218 10 al al NNP work_sny56posuffadh5a367n5vi4uq 218 11 . . . work_sny56posuffadh5a367n5vi4uq 219 1 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 219 2 21 21 CD work_sny56posuffadh5a367n5vi4uq 219 3 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 219 4 and and CC work_sny56posuffadh5a367n5vi4uq 219 5 Zhang Zhang NNP work_sny56posuffadh5a367n5vi4uq 219 6 et et NNP work_sny56posuffadh5a367n5vi4uq 219 7 al al NNP work_sny56posuffadh5a367n5vi4uq 219 8 . . . work_sny56posuffadh5a367n5vi4uq 220 1 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 220 2 22 22 CD work_sny56posuffadh5a367n5vi4uq 220 3 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 220 4 to to TO work_sny56posuffadh5a367n5vi4uq 220 5 deliver deliver VB work_sny56posuffadh5a367n5vi4uq 220 6 an an DT work_sny56posuffadh5a367n5vi4uq 220 7 SB SB NNP work_sny56posuffadh5a367n5vi4uq 220 8 transposon transposon NN work_sny56posuffadh5a367n5vi4uq 220 9 to to IN work_sny56posuffadh5a367n5vi4uq 220 10 MPS MPS NNP work_sny56posuffadh5a367n5vi4uq 220 11 VII VII NNP work_sny56posuffadh5a367n5vi4uq 220 12 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 220 13 that that WDT work_sny56posuffadh5a367n5vi4uq 220 14 were be VBD work_sny56posuffadh5a367n5vi4uq 220 15 completely completely RB work_sny56posuffadh5a367n5vi4uq 220 16 deficient deficient JJ work_sny56posuffadh5a367n5vi4uq 220 17 in in IN work_sny56posuffadh5a367n5vi4uq 220 18 lysosomal lysosomal JJ work_sny56posuffadh5a367n5vi4uq 220 19 hydrolase hydrolase NN work_sny56posuffadh5a367n5vi4uq 220 20 β β NN work_sny56posuffadh5a367n5vi4uq 220 21 - - HYPH work_sny56posuffadh5a367n5vi4uq 220 22 glucuronidase glucuronidase NNP work_sny56posuffadh5a367n5vi4uq 220 23 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 220 24 23 23 CD work_sny56posuffadh5a367n5vi4uq 220 25 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 220 26 . . . work_sny56posuffadh5a367n5vi4uq 221 1 The the DT work_sny56posuffadh5a367n5vi4uq 221 2 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 221 3 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 221 4 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 221 5 constructed construct VBD work_sny56posuffadh5a367n5vi4uq 221 6 contained contain VBD work_sny56posuffadh5a367n5vi4uq 221 7 an an DT work_sny56posuffadh5a367n5vi4uq 221 8 expression expression NN work_sny56posuffadh5a367n5vi4uq 221 9 cassette cassette NN work_sny56posuffadh5a367n5vi4uq 221 10 for for IN work_sny56posuffadh5a367n5vi4uq 221 11 β β NN work_sny56posuffadh5a367n5vi4uq 221 12 - - HYPH work_sny56posuffadh5a367n5vi4uq 221 13 glucuronidase glucuronidase NNP work_sny56posuffadh5a367n5vi4uq 221 14 CAGGS CAGGS NNP work_sny56posuffadh5a367n5vi4uq 221 15 - - HYPH work_sny56posuffadh5a367n5vi4uq 221 16 GUSB GUSB NNP work_sny56posuffadh5a367n5vi4uq 221 17 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 221 18 Aronovich Aronovich NNP work_sny56posuffadh5a367n5vi4uq 221 19 et et FW work_sny56posuffadh5a367n5vi4uq 221 20 al al NNP work_sny56posuffadh5a367n5vi4uq 221 21 . . NNP work_sny56posuffadh5a367n5vi4uq 221 22 , , , work_sny56posuffadh5a367n5vi4uq 221 23 in in IN work_sny56posuffadh5a367n5vi4uq 221 24 preparation preparation NN work_sny56posuffadh5a367n5vi4uq 221 25 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 221 26 . . . work_sny56posuffadh5a367n5vi4uq 222 1 Two two CD work_sny56posuffadh5a367n5vi4uq 222 2 groups group NNS work_sny56posuffadh5a367n5vi4uq 222 3 of of IN work_sny56posuffadh5a367n5vi4uq 222 4 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 222 5 were be VBD work_sny56posuffadh5a367n5vi4uq 222 6 injected inject VBN work_sny56posuffadh5a367n5vi4uq 222 7 with with IN work_sny56posuffadh5a367n5vi4uq 222 8 pT pT NNP work_sny56posuffadh5a367n5vi4uq 222 9 / / SYM work_sny56posuffadh5a367n5vi4uq 222 10 CAGGS CAGGS NNP work_sny56posuffadh5a367n5vi4uq 222 11 - - HYPH work_sny56posuffadh5a367n5vi4uq 222 12 GUSB GUSB NNP work_sny56posuffadh5a367n5vi4uq 222 13 : : : work_sny56posuffadh5a367n5vi4uq 222 14 Treatment Treatment NNP work_sny56posuffadh5a367n5vi4uq 222 15 Group Group NNP work_sny56posuffadh5a367n5vi4uq 222 16 1 1 CD work_sny56posuffadh5a367n5vi4uq 222 17 received receive VBD work_sny56posuffadh5a367n5vi4uq 222 18 only only RB work_sny56posuffadh5a367n5vi4uq 222 19 the the DT work_sny56posuffadh5a367n5vi4uq 222 20 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 222 21 - - HYPH work_sny56posuffadh5a367n5vi4uq 222 22 containing contain VBG work_sny56posuffadh5a367n5vi4uq 222 23 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 222 24 , , , work_sny56posuffadh5a367n5vi4uq 222 25 Treatment Treatment NNP work_sny56posuffadh5a367n5vi4uq 222 26 Group Group NNP work_sny56posuffadh5a367n5vi4uq 222 27 2 2 CD work_sny56posuffadh5a367n5vi4uq 222 28 was be VBD work_sny56posuffadh5a367n5vi4uq 222 29 co co VBN work_sny56posuffadh5a367n5vi4uq 222 30 - - VBN work_sny56posuffadh5a367n5vi4uq 222 31 injected inject VBN work_sny56posuffadh5a367n5vi4uq 222 32 with with IN work_sny56posuffadh5a367n5vi4uq 222 33 pCMVSB pcmvsb NN work_sny56posuffadh5a367n5vi4uq 222 34 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 222 35 at at IN work_sny56posuffadh5a367n5vi4uq 222 36 a a DT work_sny56posuffadh5a367n5vi4uq 222 37 molar molar JJ work_sny56posuffadh5a367n5vi4uq 222 38 ratio ratio NN work_sny56posuffadh5a367n5vi4uq 222 39 of of IN work_sny56posuffadh5a367n5vi4uq 222 40 10 10 CD work_sny56posuffadh5a367n5vi4uq 222 41 : : SYM work_sny56posuffadh5a367n5vi4uq 222 42 1 1 CD work_sny56posuffadh5a367n5vi4uq 222 43 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 222 44 / / SYM work_sny56posuffadh5a367n5vi4uq 222 45 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 222 46 . . . work_sny56posuffadh5a367n5vi4uq 223 1 As as IN work_sny56posuffadh5a367n5vi4uq 223 2 in in IN work_sny56posuffadh5a367n5vi4uq 223 3 zebrafish zebrafish NNP work_sny56posuffadh5a367n5vi4uq 223 4 , , , work_sny56posuffadh5a367n5vi4uq 223 5 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 223 6 detected detect VBD work_sny56posuffadh5a367n5vi4uq 223 7 excision excision NN work_sny56posuffadh5a367n5vi4uq 223 8 events event NNS work_sny56posuffadh5a367n5vi4uq 223 9 only only RB work_sny56posuffadh5a367n5vi4uq 223 10 when when WRB work_sny56posuffadh5a367n5vi4uq 223 11 both both CC work_sny56posuffadh5a367n5vi4uq 223 12 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 223 13 and and CC work_sny56posuffadh5a367n5vi4uq 223 14 transposase transposase NNP work_sny56posuffadh5a367n5vi4uq 223 15 were be VBD work_sny56posuffadh5a367n5vi4uq 223 16 injected inject VBN work_sny56posuffadh5a367n5vi4uq 223 17 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 223 18 Figure Figure NNP work_sny56posuffadh5a367n5vi4uq 223 19 7 7 CD work_sny56posuffadh5a367n5vi4uq 223 20 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 223 21 . . . work_sny56posuffadh5a367n5vi4uq 224 1 The the DT work_sny56posuffadh5a367n5vi4uq 224 2 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 224 3 bands band NNS work_sny56posuffadh5a367n5vi4uq 224 4 were be VBD work_sny56posuffadh5a367n5vi4uq 224 5 excised excise VBN work_sny56posuffadh5a367n5vi4uq 224 6 from from IN work_sny56posuffadh5a367n5vi4uq 224 7 the the DT work_sny56posuffadh5a367n5vi4uq 224 8 gel gel NN work_sny56posuffadh5a367n5vi4uq 224 9 for for IN work_sny56posuffadh5a367n5vi4uq 224 10 cloning clone VBG work_sny56posuffadh5a367n5vi4uq 224 11 and and CC work_sny56posuffadh5a367n5vi4uq 224 12 sequencing sequence VBG work_sny56posuffadh5a367n5vi4uq 224 13 as as IN work_sny56posuffadh5a367n5vi4uq 224 14 described describe VBN work_sny56posuffadh5a367n5vi4uq 224 15 in in IN work_sny56posuffadh5a367n5vi4uq 224 16 the the DT work_sny56posuffadh5a367n5vi4uq 224 17 previous previous JJ work_sny56posuffadh5a367n5vi4uq 224 18 section section NN work_sny56posuffadh5a367n5vi4uq 224 19 . . . work_sny56posuffadh5a367n5vi4uq 225 1 The the DT work_sny56posuffadh5a367n5vi4uq 225 2 predominant predominant JJ work_sny56posuffadh5a367n5vi4uq 225 3 band band NN work_sny56posuffadh5a367n5vi4uq 225 4 of of IN work_sny56posuffadh5a367n5vi4uq 225 5 456 456 CD work_sny56posuffadh5a367n5vi4uq 225 6 bp bp NN work_sny56posuffadh5a367n5vi4uq 225 7 yielded yield VBN work_sny56posuffadh5a367n5vi4uq 225 8 eight eight CD work_sny56posuffadh5a367n5vi4uq 225 9 readable readable JJ work_sny56posuffadh5a367n5vi4uq 225 10 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 225 11 , , , work_sny56posuffadh5a367n5vi4uq 225 12 seven seven CD work_sny56posuffadh5a367n5vi4uq 225 13 of of IN work_sny56posuffadh5a367n5vi4uq 225 14 which which WDT work_sny56posuffadh5a367n5vi4uq 225 15 gave give VBD work_sny56posuffadh5a367n5vi4uq 225 16 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 225 17 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 225 18 of of IN work_sny56posuffadh5a367n5vi4uq 225 19 TAC(A TAC(A NNP work_sny56posuffadh5a367n5vi4uq 225 20 / / SYM work_sny56posuffadh5a367n5vi4uq 225 21 T)GTA T)GTA NNP work_sny56posuffadh5a367n5vi4uq 225 22 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 225 23 Table table NN work_sny56posuffadh5a367n5vi4uq 225 24 4 4 CD work_sny56posuffadh5a367n5vi4uq 225 25 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 225 26 , , , work_sny56posuffadh5a367n5vi4uq 225 27 similar similar JJ work_sny56posuffadh5a367n5vi4uq 225 28 to to IN work_sny56posuffadh5a367n5vi4uq 225 29 those those DT work_sny56posuffadh5a367n5vi4uq 225 30 found find VBN work_sny56posuffadh5a367n5vi4uq 225 31 in in IN work_sny56posuffadh5a367n5vi4uq 225 32 zebrafish zebrafish NNP work_sny56posuffadh5a367n5vi4uq 225 33 embryos embryos NNP work_sny56posuffadh5a367n5vi4uq 225 34 and and CC work_sny56posuffadh5a367n5vi4uq 225 35 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 225 36 ES ES NNP work_sny56posuffadh5a367n5vi4uq 225 37 cells cell NNS work_sny56posuffadh5a367n5vi4uq 225 38 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 225 39 Table table NN work_sny56posuffadh5a367n5vi4uq 225 40 3 3 CD work_sny56posuffadh5a367n5vi4uq 225 41 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 225 42 and and CC work_sny56posuffadh5a367n5vi4uq 225 43 one one CD work_sny56posuffadh5a367n5vi4uq 225 44 of of IN work_sny56posuffadh5a367n5vi4uq 225 45 which which WDT work_sny56posuffadh5a367n5vi4uq 225 46 appeared appear VBD work_sny56posuffadh5a367n5vi4uq 225 47 to to TO work_sny56posuffadh5a367n5vi4uq 225 48 be be VB work_sny56posuffadh5a367n5vi4uq 225 49 a a DT work_sny56posuffadh5a367n5vi4uq 225 50 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 225 51 event event NN work_sny56posuffadh5a367n5vi4uq 225 52 using use VBG work_sny56posuffadh5a367n5vi4uq 225 53 an an DT work_sny56posuffadh5a367n5vi4uq 225 54 alternative alternative JJ work_sny56posuffadh5a367n5vi4uq 225 55 TA TA NNP work_sny56posuffadh5a367n5vi4uq 225 56 excision excision NN work_sny56posuffadh5a367n5vi4uq 225 57 site site NN work_sny56posuffadh5a367n5vi4uq 225 58 . . . work_sny56posuffadh5a367n5vi4uq 226 1 Another another DT work_sny56posuffadh5a367n5vi4uq 226 2 six six CD work_sny56posuffadh5a367n5vi4uq 226 3 events event NNS work_sny56posuffadh5a367n5vi4uq 226 4 were be VBD work_sny56posuffadh5a367n5vi4uq 226 5 sequenced sequence VBN work_sny56posuffadh5a367n5vi4uq 226 6 from from IN work_sny56posuffadh5a367n5vi4uq 226 7 smaller small JJR work_sny56posuffadh5a367n5vi4uq 226 8 , , , work_sny56posuffadh5a367n5vi4uq 226 9 minor minor JJ work_sny56posuffadh5a367n5vi4uq 226 10 bands band NNS work_sny56posuffadh5a367n5vi4uq 226 11 that that WDT work_sny56posuffadh5a367n5vi4uq 226 12 showed show VBD work_sny56posuffadh5a367n5vi4uq 226 13 deletions deletion NNS work_sny56posuffadh5a367n5vi4uq 226 14 of of IN work_sny56posuffadh5a367n5vi4uq 226 15 various various JJ work_sny56posuffadh5a367n5vi4uq 226 16 sizes size NNS work_sny56posuffadh5a367n5vi4uq 226 17 and and CC work_sny56posuffadh5a367n5vi4uq 226 18 nucleotide nucleotide JJ work_sny56posuffadh5a367n5vi4uq 226 19 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 226 20 that that WDT work_sny56posuffadh5a367n5vi4uq 226 21 indicated indicate VBD work_sny56posuffadh5a367n5vi4uq 226 22 illegitimate illegitimate JJ work_sny56posuffadh5a367n5vi4uq 226 23 B B NNP work_sny56posuffadh5a367n5vi4uq 226 24 A A NNP work_sny56posuffadh5a367n5vi4uq 226 25 Figure Figure NNP work_sny56posuffadh5a367n5vi4uq 226 26 6 6 CD work_sny56posuffadh5a367n5vi4uq 226 27 . . . work_sny56posuffadh5a367n5vi4uq 227 1 Effect effect NN work_sny56posuffadh5a367n5vi4uq 227 2 of of IN work_sny56posuffadh5a367n5vi4uq 227 3 TA TA NNP work_sny56posuffadh5a367n5vi4uq 227 4 - - HYPH work_sny56posuffadh5a367n5vi4uq 227 5 dinucleotides dinucleotide NNS work_sny56posuffadh5a367n5vi4uq 227 6 on on IN work_sny56posuffadh5a367n5vi4uq 227 7 excision excision NN work_sny56posuffadh5a367n5vi4uq 227 8 . . . work_sny56posuffadh5a367n5vi4uq 228 1 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 228 2 A a NN work_sny56posuffadh5a367n5vi4uq 228 3 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 228 4 Excision excision NN work_sny56posuffadh5a367n5vi4uq 228 5 analysis analysis NN work_sny56posuffadh5a367n5vi4uq 228 6 of of IN work_sny56posuffadh5a367n5vi4uq 228 7 TA TA NNP work_sny56posuffadh5a367n5vi4uq 228 8 mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 228 9 on on IN work_sny56posuffadh5a367n5vi4uq 228 10 either either DT work_sny56posuffadh5a367n5vi4uq 228 11 side side NN work_sny56posuffadh5a367n5vi4uq 228 12 and and CC work_sny56posuffadh5a367n5vi4uq 228 13 both both DT work_sny56posuffadh5a367n5vi4uq 228 14 sides side NNS work_sny56posuffadh5a367n5vi4uq 228 15 of of IN work_sny56posuffadh5a367n5vi4uq 228 16 the the DT work_sny56posuffadh5a367n5vi4uq 228 17 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 228 18 . . . work_sny56posuffadh5a367n5vi4uq 229 1 TA TA NNP work_sny56posuffadh5a367n5vi4uq 229 2 Lo Lo NNP work_sny56posuffadh5a367n5vi4uq 229 3 - - HYPH work_sny56posuffadh5a367n5vi4uq 229 4 RoTA RoTA NNP work_sny56posuffadh5a367n5vi4uq 229 5 indicates indicate VBZ work_sny56posuffadh5a367n5vi4uq 229 6 the the DT work_sny56posuffadh5a367n5vi4uq 229 7 pT pT NNP work_sny56posuffadh5a367n5vi4uq 229 8 / / SYM work_sny56posuffadh5a367n5vi4uq 229 9 HindIIIneo HindIIIneo NNP work_sny56posuffadh5a367n5vi4uq 229 10 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 229 11 . . . work_sny56posuffadh5a367n5vi4uq 230 1 Mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 230 2 are be VBP work_sny56posuffadh5a367n5vi4uq 230 3 underlined underline VBN work_sny56posuffadh5a367n5vi4uq 230 4 . . . work_sny56posuffadh5a367n5vi4uq 231 1 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 231 2 B b NN work_sny56posuffadh5a367n5vi4uq 231 3 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 231 4 Sequences Sequences NNPS work_sny56posuffadh5a367n5vi4uq 231 5 of of IN work_sny56posuffadh5a367n5vi4uq 231 6 the the DT work_sny56posuffadh5a367n5vi4uq 231 7 excision excision NN work_sny56posuffadh5a367n5vi4uq 231 8 site site NN work_sny56posuffadh5a367n5vi4uq 231 9 of of IN work_sny56posuffadh5a367n5vi4uq 231 10 the the DT work_sny56posuffadh5a367n5vi4uq 231 11 mutated mutate VBN work_sny56posuffadh5a367n5vi4uq 231 12 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 231 13 . . . work_sny56posuffadh5a367n5vi4uq 232 1 The the DT work_sny56posuffadh5a367n5vi4uq 232 2 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 232 3 are be VBP work_sny56posuffadh5a367n5vi4uq 232 4 underlined underline VBN work_sny56posuffadh5a367n5vi4uq 232 5 . . . work_sny56posuffadh5a367n5vi4uq 233 1 ND ND NNP work_sny56posuffadh5a367n5vi4uq 233 2 , , , work_sny56posuffadh5a367n5vi4uq 233 3 non non JJ work_sny56posuffadh5a367n5vi4uq 233 4 - - JJ work_sny56posuffadh5a367n5vi4uq 233 5 detectable detectable JJ work_sny56posuffadh5a367n5vi4uq 233 6 Copyright copyright NN work_sny56posuffadh5a367n5vi4uq 233 7   CD work_sny56posuffadh5a367n5vi4uq 233 8 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 233 9 John John NNP work_sny56posuffadh5a367n5vi4uq 233 10 Wiley Wiley NNP work_sny56posuffadh5a367n5vi4uq 233 11 & & CC work_sny56posuffadh5a367n5vi4uq 233 12 Sons Sons NNPS work_sny56posuffadh5a367n5vi4uq 233 13 , , , work_sny56posuffadh5a367n5vi4uq 233 14 Ltd. Ltd. NNP work_sny56posuffadh5a367n5vi4uq 233 15 J J NNP work_sny56posuffadh5a367n5vi4uq 233 16 Gene Gene NNP work_sny56posuffadh5a367n5vi4uq 233 17 Med Med NNP work_sny56posuffadh5a367n5vi4uq 233 18 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 233 19 ; ; : work_sny56posuffadh5a367n5vi4uq 233 20 6 6 CD work_sny56posuffadh5a367n5vi4uq 233 21 : : : work_sny56posuffadh5a367n5vi4uq 233 22 574 574 CD work_sny56posuffadh5a367n5vi4uq 233 23 – – : work_sny56posuffadh5a367n5vi4uq 233 24 583 583 CD work_sny56posuffadh5a367n5vi4uq 233 25 . . . work_sny56posuffadh5a367n5vi4uq 234 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 234 2 of of IN work_sny56posuffadh5a367n5vi4uq 234 3 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 234 4 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 234 5 Transposons Transposons NNP work_sny56posuffadh5a367n5vi4uq 234 6 581 581 NNP work_sny56posuffadh5a367n5vi4uq 234 7 Table table NN work_sny56posuffadh5a367n5vi4uq 234 8 3 3 CD work_sny56posuffadh5a367n5vi4uq 234 9 . . . work_sny56posuffadh5a367n5vi4uq 235 1 Comparison Comparison NNP work_sny56posuffadh5a367n5vi4uq 235 2 of of IN work_sny56posuffadh5a367n5vi4uq 235 3 SB SB NNP work_sny56posuffadh5a367n5vi4uq 235 4 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 235 5 in in IN work_sny56posuffadh5a367n5vi4uq 235 6 different different JJ work_sny56posuffadh5a367n5vi4uq 235 7 systems system NNS work_sny56posuffadh5a367n5vi4uq 235 8 Canonical Canonical NNP work_sny56posuffadh5a367n5vi4uq 235 9 Non non JJ work_sny56posuffadh5a367n5vi4uq 235 10 - - JJ work_sny56posuffadh5a367n5vi4uq 235 11 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 235 12 footprint footprint NN work_sny56posuffadh5a367n5vi4uq 235 13 missing miss VBG work_sny56posuffadh5a367n5vi4uq 235 14 Gaps/ gaps/ CD work_sny56posuffadh5a367n5vi4uq 235 15 Total total JJ work_sny56posuffadh5a367n5vi4uq 235 16 footprint footprint NN work_sny56posuffadh5a367n5vi4uq 235 17 1 1 CD work_sny56posuffadh5a367n5vi4uq 235 18 bp bp NN work_sny56posuffadh5a367n5vi4uq 235 19 2 2 CD work_sny56posuffadh5a367n5vi4uq 235 20 bp bp NNP work_sny56posuffadh5a367n5vi4uq 235 21 3 3 CD work_sny56posuffadh5a367n5vi4uq 235 22 bp bp NNP work_sny56posuffadh5a367n5vi4uq 235 23 Insertions Insertions NNP work_sny56posuffadh5a367n5vi4uq 235 24 events event NNS work_sny56posuffadh5a367n5vi4uq 235 25 Reference Reference NNP work_sny56posuffadh5a367n5vi4uq 235 26 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 235 27 cell cell NN work_sny56posuffadh5a367n5vi4uq 235 28 cultures culture VBZ work_sny56posuffadh5a367n5vi4uq 235 29 35 35 CD work_sny56posuffadh5a367n5vi4uq 235 30 % % NN work_sny56posuffadh5a367n5vi4uq 235 31 0 0 CD work_sny56posuffadh5a367n5vi4uq 235 32 18 18 CD work_sny56posuffadh5a367n5vi4uq 235 33 % % NN work_sny56posuffadh5a367n5vi4uq 235 34 29 29 CD work_sny56posuffadh5a367n5vi4uq 235 35 % % NN work_sny56posuffadh5a367n5vi4uq 235 36 18 18 CD work_sny56posuffadh5a367n5vi4uq 235 37 % % NN work_sny56posuffadh5a367n5vi4uq 235 38 17 17 CD work_sny56posuffadh5a367n5vi4uq 235 39 This this DT work_sny56posuffadh5a367n5vi4uq 235 40 study study NN work_sny56posuffadh5a367n5vi4uq 235 41 Zebrafish Zebrafish NNP work_sny56posuffadh5a367n5vi4uq 235 42 embryos embryo VBZ work_sny56posuffadh5a367n5vi4uq 235 43 96 96 CD work_sny56posuffadh5a367n5vi4uq 235 44 % % NN work_sny56posuffadh5a367n5vi4uq 235 45 0 0 CD work_sny56posuffadh5a367n5vi4uq 235 46 0 0 CD work_sny56posuffadh5a367n5vi4uq 235 47 0 0 CD work_sny56posuffadh5a367n5vi4uq 235 48 4 4 CD work_sny56posuffadh5a367n5vi4uq 235 49 % % NN work_sny56posuffadh5a367n5vi4uq 235 50 25 25 CD work_sny56posuffadh5a367n5vi4uq 235 51 This this DT work_sny56posuffadh5a367n5vi4uq 235 52 study study NN work_sny56posuffadh5a367n5vi4uq 235 53 and and CC work_sny56posuffadh5a367n5vi4uq 235 54 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 235 55 g g NNP work_sny56posuffadh5a367n5vi4uq 235 56 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 235 57 Mouse mouse NN work_sny56posuffadh5a367n5vi4uq 235 58 spermatids spermatid NNS work_sny56posuffadh5a367n5vi4uq 235 59 0 0 CD work_sny56posuffadh5a367n5vi4uq 235 60 31 31 CD work_sny56posuffadh5a367n5vi4uq 235 61 % % NN work_sny56posuffadh5a367n5vi4uq 235 62 44 44 CD work_sny56posuffadh5a367n5vi4uq 235 63 % % NN work_sny56posuffadh5a367n5vi4uq 235 64 0 0 CD work_sny56posuffadh5a367n5vi4uq 235 65 25 25 CD work_sny56posuffadh5a367n5vi4uq 235 66 % % NN work_sny56posuffadh5a367n5vi4uq 235 67 16 16 CD work_sny56posuffadh5a367n5vi4uq 235 68 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 235 69 4 4 CD work_sny56posuffadh5a367n5vi4uq 235 70 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 235 71 Mouse Mouse NNP work_sny56posuffadh5a367n5vi4uq 235 72 ES ES NNP work_sny56posuffadh5a367n5vi4uq 235 73 cells cell VBZ work_sny56posuffadh5a367n5vi4uq 235 74 85 85 CD work_sny56posuffadh5a367n5vi4uq 235 75 % % NN work_sny56posuffadh5a367n5vi4uq 235 76 0 0 CD work_sny56posuffadh5a367n5vi4uq 235 77 0 0 CD work_sny56posuffadh5a367n5vi4uq 235 78 8 8 CD work_sny56posuffadh5a367n5vi4uq 235 79 % % NN work_sny56posuffadh5a367n5vi4uq 235 80 8 8 CD work_sny56posuffadh5a367n5vi4uq 235 81 % % NN work_sny56posuffadh5a367n5vi4uq 235 82 13 13 CD work_sny56posuffadh5a367n5vi4uq 235 83 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 235 84 14 14 CD work_sny56posuffadh5a367n5vi4uq 235 85 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 235 86 Mouse Mouse NNP work_sny56posuffadh5a367n5vi4uq 235 87 Livers Livers NNP work_sny56posuffadh5a367n5vi4uq 235 88 88 88 CD work_sny56posuffadh5a367n5vi4uq 235 89 % % NN work_sny56posuffadh5a367n5vi4uq 235 90 0 0 CD work_sny56posuffadh5a367n5vi4uq 235 91 0 0 CD work_sny56posuffadh5a367n5vi4uq 235 92 0 0 CD work_sny56posuffadh5a367n5vi4uq 235 93 12 12 CD work_sny56posuffadh5a367n5vi4uq 235 94 % % NN work_sny56posuffadh5a367n5vi4uq 235 95 8 8 CD work_sny56posuffadh5a367n5vi4uq 235 96 This this DT work_sny56posuffadh5a367n5vi4uq 235 97 study study NN work_sny56posuffadh5a367n5vi4uq 235 98 Table table NN work_sny56posuffadh5a367n5vi4uq 235 99 4 4 CD work_sny56posuffadh5a367n5vi4uq 235 100 . . . work_sny56posuffadh5a367n5vi4uq 236 1 Footprint footprint NN work_sny56posuffadh5a367n5vi4uq 236 2 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 236 3 from from IN work_sny56posuffadh5a367n5vi4uq 236 4 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 236 5 livers liver NNS work_sny56posuffadh5a367n5vi4uq 236 6 Left leave VBN work_sny56posuffadh5a367n5vi4uq 236 7 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 8 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 236 9 Footprint Footprint NNP work_sny56posuffadh5a367n5vi4uq 236 10 Right right RB work_sny56posuffadh5a367n5vi4uq 236 11 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 12 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 236 13 Events event NNS work_sny56posuffadh5a367n5vi4uq 236 14 Major Major NNP work_sny56posuffadh5a367n5vi4uq 236 15 Band Band NNP work_sny56posuffadh5a367n5vi4uq 236 16 of of IN work_sny56posuffadh5a367n5vi4uq 236 17 expected expect VBN work_sny56posuffadh5a367n5vi4uq 236 18 size size NN work_sny56posuffadh5a367n5vi4uq 236 19 ∼456 ∼456 NNP work_sny56posuffadh5a367n5vi4uq 236 20 bp bp NNP work_sny56posuffadh5a367n5vi4uq 236 21 Canonical Canonical NNP work_sny56posuffadh5a367n5vi4uq 236 22 GACTCACTATAGGGCGAATTGGAGCTCGGTACCC GACTCACTATAGGGCGAATTGGAGCTCGGTACCC NNP work_sny56posuffadh5a367n5vi4uq 236 23 TA TA NNP work_sny56posuffadh5a367n5vi4uq 236 24 CAG CAG NNP work_sny56posuffadh5a367n5vi4uq 236 25 TA TA NNP work_sny56posuffadh5a367n5vi4uq 236 26 GGGGATCCTCTAGCTAGAGT GGGGATCCTCTAGCTAGAGT NNP work_sny56posuffadh5a367n5vi4uq 236 27 6 6 CD work_sny56posuffadh5a367n5vi4uq 236 28 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 236 29 GACTCACTATAGGGCGAATTGGAGCTCGGTACCC GACTCACTATAGGGCGAATTGGAGCTCGGTACCC NNP work_sny56posuffadh5a367n5vi4uq 236 30 TA TA NNP work_sny56posuffadh5a367n5vi4uq 236 31 CTG CTG NNP work_sny56posuffadh5a367n5vi4uq 236 32 TA TA NNP work_sny56posuffadh5a367n5vi4uq 236 33 GGGGATCCTCTAGCTAGAGT ggggatcctctagctagagt NN work_sny56posuffadh5a367n5vi4uq 236 34 1 1 CD work_sny56posuffadh5a367n5vi4uq 236 35 Gaps Gaps NNP work_sny56posuffadh5a367n5vi4uq 236 36 GACTCACTA- GACTCACTA- NNP work_sny56posuffadh5a367n5vi4uq 236 37 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 38 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 39 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 40 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 41 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 42 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 43 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 44 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 45 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 46 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 47 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 48 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 49 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 50 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 51 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 52 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 53 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 54 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 55 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 56 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 57 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 58 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 59 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 60 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 61 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 62 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 63 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 64 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 65 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 66 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 67 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 68 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 69 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 70 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 71 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 72 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 73 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 74 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 75 TAGAGT tagagt NN work_sny56posuffadh5a367n5vi4uq 236 76 1 1 CD work_sny56posuffadh5a367n5vi4uq 236 77 Minor Minor NNP work_sny56posuffadh5a367n5vi4uq 236 78 Bands Bands NNPS work_sny56posuffadh5a367n5vi4uq 236 79 of of IN work_sny56posuffadh5a367n5vi4uq 236 80 smaller small JJR work_sny56posuffadh5a367n5vi4uq 236 81 size size NN work_sny56posuffadh5a367n5vi4uq 236 82 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 236 83 82 82 CD work_sny56posuffadh5a367n5vi4uq 236 84 bp bp NN work_sny56posuffadh5a367n5vi4uq 236 85 deletion deletion NN work_sny56posuffadh5a367n5vi4uq 236 86 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 236 87 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 88 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 89 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 90 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 91 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 92 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 93 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 94 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 236 95 36 36 CD work_sny56posuffadh5a367n5vi4uq 236 96 bp bp NN work_sny56posuffadh5a367n5vi4uq 236 97 deletion deletion NN work_sny56posuffadh5a367n5vi4uq 236 98 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 236 99 1 1 CD work_sny56posuffadh5a367n5vi4uq 236 100 Deletions deletion NNS work_sny56posuffadh5a367n5vi4uq 236 101 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 236 102 87 87 CD work_sny56posuffadh5a367n5vi4uq 236 103 bp bp NN work_sny56posuffadh5a367n5vi4uq 236 104 deletion deletion NN work_sny56posuffadh5a367n5vi4uq 236 105 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 236 106 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 107 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 108 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 109 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 110 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 111 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 112 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 113 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 236 114 40 40 CD work_sny56posuffadh5a367n5vi4uq 236 115 bp bp NN work_sny56posuffadh5a367n5vi4uq 236 116 deletion deletion NN work_sny56posuffadh5a367n5vi4uq 236 117 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 236 118 1 1 CD work_sny56posuffadh5a367n5vi4uq 236 119 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 236 120 96 96 CD work_sny56posuffadh5a367n5vi4uq 236 121 bp bp NN work_sny56posuffadh5a367n5vi4uq 236 122 deletion deletion NN work_sny56posuffadh5a367n5vi4uq 236 123 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 236 124 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 125 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 126 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 127 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 128 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 129 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 130 - - HYPH work_sny56posuffadh5a367n5vi4uq 236 131 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 236 132 76 76 CD work_sny56posuffadh5a367n5vi4uq 236 133 bp bp NN work_sny56posuffadh5a367n5vi4uq 236 134 deletion deletion NN work_sny56posuffadh5a367n5vi4uq 236 135 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 236 136 1 1 CD work_sny56posuffadh5a367n5vi4uq 236 137 Random Random NNP work_sny56posuffadh5a367n5vi4uq 236 138 54 54 CD work_sny56posuffadh5a367n5vi4uq 236 139 bp bp NN work_sny56posuffadh5a367n5vi4uq 236 140 upstream upstream RB work_sny56posuffadh5a367n5vi4uq 236 141 of of IN work_sny56posuffadh5a367n5vi4uq 236 142 left left JJ work_sny56posuffadh5a367n5vi4uq 236 143 TA TA NNP work_sny56posuffadh5a367n5vi4uq 236 144 , , , work_sny56posuffadh5a367n5vi4uq 236 145 sequence sequence NN work_sny56posuffadh5a367n5vi4uq 236 146 continues continue VBZ work_sny56posuffadh5a367n5vi4uq 236 147 at at IN work_sny56posuffadh5a367n5vi4uq 236 148 right right RB work_sny56posuffadh5a367n5vi4uq 236 149 ITR ITR NNP work_sny56posuffadh5a367n5vi4uq 236 150 . . . work_sny56posuffadh5a367n5vi4uq 237 1 1 1 CD work_sny56posuffadh5a367n5vi4uq 237 2 insertion insertion NN work_sny56posuffadh5a367n5vi4uq 237 3 78 78 CD work_sny56posuffadh5a367n5vi4uq 237 4 bp bp NN work_sny56posuffadh5a367n5vi4uq 237 5 upstream upstream RB work_sny56posuffadh5a367n5vi4uq 237 6 of of IN work_sny56posuffadh5a367n5vi4uq 237 7 left left JJ work_sny56posuffadh5a367n5vi4uq 237 8 TA TA NNP work_sny56posuffadh5a367n5vi4uq 237 9 1 1 CD work_sny56posuffadh5a367n5vi4uq 237 10 82 82 CD work_sny56posuffadh5a367n5vi4uq 237 11 bp bp NN work_sny56posuffadh5a367n5vi4uq 237 12 upstream upstream RB work_sny56posuffadh5a367n5vi4uq 237 13 of of IN work_sny56posuffadh5a367n5vi4uq 237 14 left left JJ work_sny56posuffadh5a367n5vi4uq 237 15 TA TA NNP work_sny56posuffadh5a367n5vi4uq 237 16 continues continue VBZ work_sny56posuffadh5a367n5vi4uq 237 17 into into IN work_sny56posuffadh5a367n5vi4uq 237 18 the the DT work_sny56posuffadh5a367n5vi4uq 237 19 complementary complementary JJ work_sny56posuffadh5a367n5vi4uq 237 20 strand strand NNP work_sny56posuffadh5a367n5vi4uq 237 21 44 44 CD work_sny56posuffadh5a367n5vi4uq 237 22 bp bp NNP work_sny56posuffadh5a367n5vi4uq 237 23 downstream downstream NNP work_sny56posuffadh5a367n5vi4uq 237 24 the the DT work_sny56posuffadh5a367n5vi4uq 237 25 right right JJ work_sny56posuffadh5a367n5vi4uq 237 26 TA TA NNP work_sny56posuffadh5a367n5vi4uq 237 27 1 1 CD work_sny56posuffadh5a367n5vi4uq 237 28 500 500 CD work_sny56posuffadh5a367n5vi4uq 237 29 1 1 CD work_sny56posuffadh5a367n5vi4uq 237 30 2 2 CD work_sny56posuffadh5a367n5vi4uq 237 31 3 3 CD work_sny56posuffadh5a367n5vi4uq 237 32 4 4 CD work_sny56posuffadh5a367n5vi4uq 237 33 400 400 CD work_sny56posuffadh5a367n5vi4uq 237 34 Figure figure NN work_sny56posuffadh5a367n5vi4uq 237 35 7 7 CD work_sny56posuffadh5a367n5vi4uq 237 36 . . . work_sny56posuffadh5a367n5vi4uq 238 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 238 2 products product NNS work_sny56posuffadh5a367n5vi4uq 238 3 in in IN work_sny56posuffadh5a367n5vi4uq 238 4 the the DT work_sny56posuffadh5a367n5vi4uq 238 5 livers liver NNS work_sny56posuffadh5a367n5vi4uq 238 6 of of IN work_sny56posuffadh5a367n5vi4uq 238 7 SB SB NNP work_sny56posuffadh5a367n5vi4uq 238 8 - - HYPH work_sny56posuffadh5a367n5vi4uq 238 9 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 238 10 - - HYPH work_sny56posuffadh5a367n5vi4uq 238 11 trea- trea- NNP work_sny56posuffadh5a367n5vi4uq 238 12 ted ted NNP work_sny56posuffadh5a367n5vi4uq 238 13 MPS MPS NNP work_sny56posuffadh5a367n5vi4uq 238 14 VII VII NNP work_sny56posuffadh5a367n5vi4uq 238 15 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 238 16 . . . work_sny56posuffadh5a367n5vi4uq 239 1 Lane Lane NNP work_sny56posuffadh5a367n5vi4uq 239 2 1 1 CD work_sny56posuffadh5a367n5vi4uq 239 3 , , , work_sny56posuffadh5a367n5vi4uq 239 4 100-bp 100-bp CD work_sny56posuffadh5a367n5vi4uq 239 5 interval interval NN work_sny56posuffadh5a367n5vi4uq 239 6 markers marker NNS work_sny56posuffadh5a367n5vi4uq 239 7 ; ; : work_sny56posuffadh5a367n5vi4uq 239 8 lane lane NN work_sny56posuffadh5a367n5vi4uq 239 9 2 2 CD work_sny56posuffadh5a367n5vi4uq 239 10 , , , work_sny56posuffadh5a367n5vi4uq 239 11 treatment treatment NN work_sny56posuffadh5a367n5vi4uq 239 12 with with IN work_sny56posuffadh5a367n5vi4uq 239 13 pT pT NNP work_sny56posuffadh5a367n5vi4uq 239 14 / / SYM work_sny56posuffadh5a367n5vi4uq 239 15 CAGGS CAGGS NNP work_sny56posuffadh5a367n5vi4uq 239 16 - - HYPH work_sny56posuffadh5a367n5vi4uq 239 17 GUSB GUSB NNP work_sny56posuffadh5a367n5vi4uq 239 18 alone alone RB work_sny56posuffadh5a367n5vi4uq 239 19 ; ; : work_sny56posuffadh5a367n5vi4uq 239 20 lane lane NNP work_sny56posuffadh5a367n5vi4uq 239 21 3 3 CD work_sny56posuffadh5a367n5vi4uq 239 22 , , , work_sny56posuffadh5a367n5vi4uq 239 23 treatment treatment NN work_sny56posuffadh5a367n5vi4uq 239 24 with with IN work_sny56posuffadh5a367n5vi4uq 239 25 pT pT NNP work_sny56posuffadh5a367n5vi4uq 239 26 / / SYM work_sny56posuffadh5a367n5vi4uq 239 27 CAGGS CAGGS NNP work_sny56posuffadh5a367n5vi4uq 239 28 - - HYPH work_sny56posuffadh5a367n5vi4uq 239 29 GUSB GUSB NNP work_sny56posuffadh5a367n5vi4uq 239 30 + + SYM work_sny56posuffadh5a367n5vi4uq 239 31 pCMVSB pcmvsb NN work_sny56posuffadh5a367n5vi4uq 239 32 , , , work_sny56posuffadh5a367n5vi4uq 239 33 lane lane NNP work_sny56posuffadh5a367n5vi4uq 239 34 4 4 CD work_sny56posuffadh5a367n5vi4uq 239 35 , , , work_sny56posuffadh5a367n5vi4uq 239 36 sham sham JJ work_sny56posuffadh5a367n5vi4uq 239 37 - - HYPH work_sny56posuffadh5a367n5vi4uq 239 38 treatment treatment NN work_sny56posuffadh5a367n5vi4uq 239 39 with with IN work_sny56posuffadh5a367n5vi4uq 239 40 pBluscript pBluscript NNP work_sny56posuffadh5a367n5vi4uq 239 41 recombination recombination NN work_sny56posuffadh5a367n5vi4uq 239 42 that that WDT work_sny56posuffadh5a367n5vi4uq 239 43 did do VBD work_sny56posuffadh5a367n5vi4uq 239 44 not not RB work_sny56posuffadh5a367n5vi4uq 239 45 use use VB work_sny56posuffadh5a367n5vi4uq 239 46 SB SB NNP work_sny56posuffadh5a367n5vi4uq 239 47 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 239 48 . . . work_sny56posuffadh5a367n5vi4uq 240 1 Taken take VBN work_sny56posuffadh5a367n5vi4uq 240 2 together together RB work_sny56posuffadh5a367n5vi4uq 240 3 , , , work_sny56posuffadh5a367n5vi4uq 240 4 the the DT work_sny56posuffadh5a367n5vi4uq 240 5 data datum NNS work_sny56posuffadh5a367n5vi4uq 240 6 suggest suggest VBP work_sny56posuffadh5a367n5vi4uq 240 7 that that IN work_sny56posuffadh5a367n5vi4uq 240 8 the the DT work_sny56posuffadh5a367n5vi4uq 240 9 excision excision NN work_sny56posuffadh5a367n5vi4uq 240 10 assay assay NN work_sny56posuffadh5a367n5vi4uq 240 11 is be VBZ work_sny56posuffadh5a367n5vi4uq 240 12 useful useful JJ work_sny56posuffadh5a367n5vi4uq 240 13 for for IN work_sny56posuffadh5a367n5vi4uq 240 14 quickly quickly RB work_sny56posuffadh5a367n5vi4uq 240 15 evaluating evaluate VBG work_sny56posuffadh5a367n5vi4uq 240 16 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 240 17 of of IN work_sny56posuffadh5a367n5vi4uq 240 18 transgenes transgene NNS work_sny56posuffadh5a367n5vi4uq 240 19 into into IN work_sny56posuffadh5a367n5vi4uq 240 20 organs organ NNS work_sny56posuffadh5a367n5vi4uq 240 21 and and CC work_sny56posuffadh5a367n5vi4uq 240 22 tissues tissue NNS work_sny56posuffadh5a367n5vi4uq 240 23 of of IN work_sny56posuffadh5a367n5vi4uq 240 24 living living NN work_sny56posuffadh5a367n5vi4uq 240 25 animals animal NNS work_sny56posuffadh5a367n5vi4uq 240 26 . . . work_sny56posuffadh5a367n5vi4uq 241 1 Discussion Discussion NNP work_sny56posuffadh5a367n5vi4uq 241 2 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 241 3 developed develop VBD work_sny56posuffadh5a367n5vi4uq 241 4 a a DT work_sny56posuffadh5a367n5vi4uq 241 5 plasmid plasmid NN work_sny56posuffadh5a367n5vi4uq 241 6 - - HYPH work_sny56posuffadh5a367n5vi4uq 241 7 based base VBN work_sny56posuffadh5a367n5vi4uq 241 8 excision excision NN work_sny56posuffadh5a367n5vi4uq 241 9 assay assay NN work_sny56posuffadh5a367n5vi4uq 241 10 for for IN work_sny56posuffadh5a367n5vi4uq 241 11 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 241 12 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 241 13 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 241 14 SB)-mediated sb)-mediated CD work_sny56posuffadh5a367n5vi4uq 241 15 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 241 16 and and CC work_sny56posuffadh5a367n5vi4uq 241 17 confirm confirm VB work_sny56posuffadh5a367n5vi4uq 241 18 preliminary preliminary JJ work_sny56posuffadh5a367n5vi4uq 241 19 findings finding NNS work_sny56posuffadh5a367n5vi4uq 241 20 that that WDT work_sny56posuffadh5a367n5vi4uq 241 21 suggested suggest VBD work_sny56posuffadh5a367n5vi4uq 241 22 that that IN work_sny56posuffadh5a367n5vi4uq 241 23 excision excision NN work_sny56posuffadh5a367n5vi4uq 241 24 rates rate NNS work_sny56posuffadh5a367n5vi4uq 241 25 correlate correlate VBP work_sny56posuffadh5a367n5vi4uq 241 26 to to IN work_sny56posuffadh5a367n5vi4uq 241 27 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 241 28 rates rate NNS work_sny56posuffadh5a367n5vi4uq 241 29 . . . work_sny56posuffadh5a367n5vi4uq 242 1 Here here RB work_sny56posuffadh5a367n5vi4uq 242 2 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 242 3 have have VBP work_sny56posuffadh5a367n5vi4uq 242 4 used use VBN work_sny56posuffadh5a367n5vi4uq 242 5 the the DT work_sny56posuffadh5a367n5vi4uq 242 6 excision excision NN work_sny56posuffadh5a367n5vi4uq 242 7 assay assay NN work_sny56posuffadh5a367n5vi4uq 242 8 as as IN work_sny56posuffadh5a367n5vi4uq 242 9 a a DT work_sny56posuffadh5a367n5vi4uq 242 10 simplified simplified JJ work_sny56posuffadh5a367n5vi4uq 242 11 method method NN work_sny56posuffadh5a367n5vi4uq 242 12 to to TO work_sny56posuffadh5a367n5vi4uq 242 13 determine determine VB work_sny56posuffadh5a367n5vi4uq 242 14 the the DT work_sny56posuffadh5a367n5vi4uq 242 15 outcome outcome NN work_sny56posuffadh5a367n5vi4uq 242 16 of of IN work_sny56posuffadh5a367n5vi4uq 242 17 the the DT work_sny56posuffadh5a367n5vi4uq 242 18 multi multi JJ work_sny56posuffadh5a367n5vi4uq 242 19 - - JJ work_sny56posuffadh5a367n5vi4uq 242 20 step step JJ work_sny56posuffadh5a367n5vi4uq 242 21 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 242 22 process process NN work_sny56posuffadh5a367n5vi4uq 242 23 and and CC work_sny56posuffadh5a367n5vi4uq 242 24 to to TO work_sny56posuffadh5a367n5vi4uq 242 25 facilitate facilitate VB work_sny56posuffadh5a367n5vi4uq 242 26 our -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 242 27 understanding understanding NN work_sny56posuffadh5a367n5vi4uq 242 28 of of IN work_sny56posuffadh5a367n5vi4uq 242 29 the the DT work_sny56posuffadh5a367n5vi4uq 242 30 cis cis NN work_sny56posuffadh5a367n5vi4uq 242 31 - - HYPH work_sny56posuffadh5a367n5vi4uq 242 32 elements element NNS work_sny56posuffadh5a367n5vi4uq 242 33 required require VBN work_sny56posuffadh5a367n5vi4uq 242 34 for for IN work_sny56posuffadh5a367n5vi4uq 242 35 SB SB NNP work_sny56posuffadh5a367n5vi4uq 242 36 excision excision NN work_sny56posuffadh5a367n5vi4uq 242 37 . . . work_sny56posuffadh5a367n5vi4uq 243 1 This this DT work_sny56posuffadh5a367n5vi4uq 243 2 PCR PCR NNP work_sny56posuffadh5a367n5vi4uq 243 3 - - HYPH work_sny56posuffadh5a367n5vi4uq 243 4 based base VBN work_sny56posuffadh5a367n5vi4uq 243 5 excision excision NN work_sny56posuffadh5a367n5vi4uq 243 6 assay assay NN work_sny56posuffadh5a367n5vi4uq 243 7 is be VBZ work_sny56posuffadh5a367n5vi4uq 243 8 independent independent JJ work_sny56posuffadh5a367n5vi4uq 243 9 of of IN work_sny56posuffadh5a367n5vi4uq 243 10 the the DT work_sny56posuffadh5a367n5vi4uq 243 11 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 243 12 content content NN work_sny56posuffadh5a367n5vi4uq 243 13 and and CC work_sny56posuffadh5a367n5vi4uq 243 14 has have VBZ work_sny56posuffadh5a367n5vi4uq 243 15 shown show VBN work_sny56posuffadh5a367n5vi4uq 243 16 that that IN work_sny56posuffadh5a367n5vi4uq 243 17 the the DT work_sny56posuffadh5a367n5vi4uq 243 18 excision excision NN work_sny56posuffadh5a367n5vi4uq 243 19 assay assay NN work_sny56posuffadh5a367n5vi4uq 243 20 can can MD work_sny56posuffadh5a367n5vi4uq 243 21 be be VB work_sny56posuffadh5a367n5vi4uq 243 22 used use VBN work_sny56posuffadh5a367n5vi4uq 243 23 to to TO work_sny56posuffadh5a367n5vi4uq 243 24 monitor monitor VB work_sny56posuffadh5a367n5vi4uq 243 25 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 243 26 in in IN work_sny56posuffadh5a367n5vi4uq 243 27 systems system NNS work_sny56posuffadh5a367n5vi4uq 243 28 wherein wherein WRB work_sny56posuffadh5a367n5vi4uq 243 29 drug drug NN work_sny56posuffadh5a367n5vi4uq 243 30 selection selection NN work_sny56posuffadh5a367n5vi4uq 243 31 is be VBZ work_sny56posuffadh5a367n5vi4uq 243 32 not not RB work_sny56posuffadh5a367n5vi4uq 243 33 feasible feasible JJ work_sny56posuffadh5a367n5vi4uq 243 34 , , , work_sny56posuffadh5a367n5vi4uq 243 35 such such JJ work_sny56posuffadh5a367n5vi4uq 243 36 as as IN work_sny56posuffadh5a367n5vi4uq 243 37 in in IN work_sny56posuffadh5a367n5vi4uq 243 38 non non JJ work_sny56posuffadh5a367n5vi4uq 243 39 - - JJ work_sny56posuffadh5a367n5vi4uq 243 40 dividing dividing JJ work_sny56posuffadh5a367n5vi4uq 243 41 cells cell NNS work_sny56posuffadh5a367n5vi4uq 243 42 of of IN work_sny56posuffadh5a367n5vi4uq 243 43 whole whole JJ work_sny56posuffadh5a367n5vi4uq 243 44 animal animal NN work_sny56posuffadh5a367n5vi4uq 243 45 tissues tissue NNS work_sny56posuffadh5a367n5vi4uq 243 46 . . . work_sny56posuffadh5a367n5vi4uq 244 1 Thus thus RB work_sny56posuffadh5a367n5vi4uq 244 2 , , , work_sny56posuffadh5a367n5vi4uq 244 3 the the DT work_sny56posuffadh5a367n5vi4uq 244 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 244 5 assay assay NN work_sny56posuffadh5a367n5vi4uq 244 6 offers offer VBZ work_sny56posuffadh5a367n5vi4uq 244 7 a a DT work_sny56posuffadh5a367n5vi4uq 244 8 high high JJ work_sny56posuffadh5a367n5vi4uq 244 9 - - HYPH work_sny56posuffadh5a367n5vi4uq 244 10 throughput throughput NN work_sny56posuffadh5a367n5vi4uq 244 11 means mean NNS work_sny56posuffadh5a367n5vi4uq 244 12 to to TO work_sny56posuffadh5a367n5vi4uq 244 13 detect detect VB work_sny56posuffadh5a367n5vi4uq 244 14 and and CC work_sny56posuffadh5a367n5vi4uq 244 15 measure measure VB work_sny56posuffadh5a367n5vi4uq 244 16 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 244 17 in in IN work_sny56posuffadh5a367n5vi4uq 244 18 somatic somatic JJ work_sny56posuffadh5a367n5vi4uq 244 19 tissues tissue NNS work_sny56posuffadh5a367n5vi4uq 244 20 in in IN work_sny56posuffadh5a367n5vi4uq 244 21 which which WDT work_sny56posuffadh5a367n5vi4uq 244 22 multiple multiple JJ work_sny56posuffadh5a367n5vi4uq 244 23 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 244 24 events event NNS work_sny56posuffadh5a367n5vi4uq 244 25 occur occur VBP work_sny56posuffadh5a367n5vi4uq 244 26 in in IN work_sny56posuffadh5a367n5vi4uq 244 27 a a DT work_sny56posuffadh5a367n5vi4uq 244 28 large large JJ work_sny56posuffadh5a367n5vi4uq 244 29 number number NN work_sny56posuffadh5a367n5vi4uq 244 30 of of IN work_sny56posuffadh5a367n5vi4uq 244 31 cells cell NNS work_sny56posuffadh5a367n5vi4uq 244 32 . . . work_sny56posuffadh5a367n5vi4uq 245 1 The the DT work_sny56posuffadh5a367n5vi4uq 245 2 excision excision NN work_sny56posuffadh5a367n5vi4uq 245 3 assay assay NN work_sny56posuffadh5a367n5vi4uq 245 4 was be VBD work_sny56posuffadh5a367n5vi4uq 245 5 used use VBN work_sny56posuffadh5a367n5vi4uq 245 6 to to TO work_sny56posuffadh5a367n5vi4uq 245 7 elucidate elucidate VB work_sny56posuffadh5a367n5vi4uq 245 8 several several JJ work_sny56posuffadh5a367n5vi4uq 245 9 param- param- JJ work_sny56posuffadh5a367n5vi4uq 245 10 eters eter NNS work_sny56posuffadh5a367n5vi4uq 245 11 of of IN work_sny56posuffadh5a367n5vi4uq 245 12 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 245 13 that that WDT work_sny56posuffadh5a367n5vi4uq 245 14 have have VBP work_sny56posuffadh5a367n5vi4uq 245 15 not not RB work_sny56posuffadh5a367n5vi4uq 245 16 been be VBN work_sny56posuffadh5a367n5vi4uq 245 17 appreciated appreciate VBN work_sny56posuffadh5a367n5vi4uq 245 18 before before RB work_sny56posuffadh5a367n5vi4uq 245 19 . . . work_sny56posuffadh5a367n5vi4uq 246 1 The the DT work_sny56posuffadh5a367n5vi4uq 246 2 first first JJ work_sny56posuffadh5a367n5vi4uq 246 3 involves involve VBZ work_sny56posuffadh5a367n5vi4uq 246 4 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 246 5 left leave VBN work_sny56posuffadh5a367n5vi4uq 246 6 in in IN work_sny56posuffadh5a367n5vi4uq 246 7 the the DT work_sny56posuffadh5a367n5vi4uq 246 8 exci- exci- JJ work_sny56posuffadh5a367n5vi4uq 246 9 sion sion NN work_sny56posuffadh5a367n5vi4uq 246 10 site site NN work_sny56posuffadh5a367n5vi4uq 246 11 . . . work_sny56posuffadh5a367n5vi4uq 247 1 The the DT work_sny56posuffadh5a367n5vi4uq 247 2 ability ability NN work_sny56posuffadh5a367n5vi4uq 247 3 to to TO work_sny56posuffadh5a367n5vi4uq 247 4 revert revert VB work_sny56posuffadh5a367n5vi4uq 247 5 the the DT work_sny56posuffadh5a367n5vi4uq 247 6 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 247 7 event event NN work_sny56posuffadh5a367n5vi4uq 247 8 after after IN work_sny56posuffadh5a367n5vi4uq 247 9 remobilization remobilization NN work_sny56posuffadh5a367n5vi4uq 247 10 of of IN work_sny56posuffadh5a367n5vi4uq 247 11 the the DT work_sny56posuffadh5a367n5vi4uq 247 12 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 247 13 is be VBZ work_sny56posuffadh5a367n5vi4uq 247 14 one one CD work_sny56posuffadh5a367n5vi4uq 247 15 potential potential JJ work_sny56posuffadh5a367n5vi4uq 247 16 advantage advantage NN work_sny56posuffadh5a367n5vi4uq 247 17 of of IN work_sny56posuffadh5a367n5vi4uq 247 18 using use VBG work_sny56posuffadh5a367n5vi4uq 247 19 transposable transposable JJ work_sny56posuffadh5a367n5vi4uq 247 20 elements element NNS work_sny56posuffadh5a367n5vi4uq 247 21 in in IN work_sny56posuffadh5a367n5vi4uq 247 22 functional functional JJ work_sny56posuffadh5a367n5vi4uq 247 23 genomics genomic NNS work_sny56posuffadh5a367n5vi4uq 247 24 . . . work_sny56posuffadh5a367n5vi4uq 248 1 Consequently consequently RB work_sny56posuffadh5a367n5vi4uq 248 2 , , , work_sny56posuffadh5a367n5vi4uq 248 3 it -PRON- PRP work_sny56posuffadh5a367n5vi4uq 248 4 is be VBZ work_sny56posuffadh5a367n5vi4uq 248 5 important important JJ work_sny56posuffadh5a367n5vi4uq 248 6 to to TO work_sny56posuffadh5a367n5vi4uq 248 7 know know VB work_sny56posuffadh5a367n5vi4uq 248 8 whether whether IN work_sny56posuffadh5a367n5vi4uq 248 9 the the DT work_sny56posuffadh5a367n5vi4uq 248 10 footprint footprint NN work_sny56posuffadh5a367n5vi4uq 248 11 left leave VBD work_sny56posuffadh5a367n5vi4uq 248 12 after after IN work_sny56posuffadh5a367n5vi4uq 248 13 excision excision NN work_sny56posuffadh5a367n5vi4uq 248 14 would would MD work_sny56posuffadh5a367n5vi4uq 248 15 maintain maintain VB work_sny56posuffadh5a367n5vi4uq 248 16 the the DT work_sny56posuffadh5a367n5vi4uq 248 17 open open JJ work_sny56posuffadh5a367n5vi4uq 248 18 reading reading NN work_sny56posuffadh5a367n5vi4uq 248 19 frame frame NN work_sny56posuffadh5a367n5vi4uq 248 20 for for IN work_sny56posuffadh5a367n5vi4uq 248 21 translation translation NN work_sny56posuffadh5a367n5vi4uq 248 22 . . . work_sny56posuffadh5a367n5vi4uq 249 1 Two two CD work_sny56posuffadh5a367n5vi4uq 249 2 previous previous JJ work_sny56posuffadh5a367n5vi4uq 249 3 studies study NNS work_sny56posuffadh5a367n5vi4uq 249 4 have have VBP work_sny56posuffadh5a367n5vi4uq 249 5 provided provide VBN work_sny56posuffadh5a367n5vi4uq 249 6 conflicting conflicting JJ work_sny56posuffadh5a367n5vi4uq 249 7 results result NNS work_sny56posuffadh5a367n5vi4uq 249 8 regarding regard VBG work_sny56posuffadh5a367n5vi4uq 249 9 the the DT work_sny56posuffadh5a367n5vi4uq 249 10 SB SB NNP work_sny56posuffadh5a367n5vi4uq 249 11 footprint footprint NN work_sny56posuffadh5a367n5vi4uq 249 12 . . . work_sny56posuffadh5a367n5vi4uq 250 1 Luo Luo NNP work_sny56posuffadh5a367n5vi4uq 250 2 et et NNP work_sny56posuffadh5a367n5vi4uq 250 3 al al NNP work_sny56posuffadh5a367n5vi4uq 250 4 . . . work_sny56posuffadh5a367n5vi4uq 251 1 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 251 2 14 14 CD work_sny56posuffadh5a367n5vi4uq 251 3 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 251 4 observed observe VBN work_sny56posuffadh5a367n5vi4uq 251 5 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 251 6 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 251 7 in in IN work_sny56posuffadh5a367n5vi4uq 251 8 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 251 9 embryonic embryonic JJ work_sny56posuffadh5a367n5vi4uq 251 10 stem stem NN work_sny56posuffadh5a367n5vi4uq 251 11 cells cell NNS work_sny56posuffadh5a367n5vi4uq 251 12 , , , work_sny56posuffadh5a367n5vi4uq 251 13 whereas whereas IN work_sny56posuffadh5a367n5vi4uq 251 14 Fischer Fischer NNP work_sny56posuffadh5a367n5vi4uq 251 15 et et NNP work_sny56posuffadh5a367n5vi4uq 251 16 al al NNP work_sny56posuffadh5a367n5vi4uq 251 17 . . . work_sny56posuffadh5a367n5vi4uq 252 1 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 252 2 4 4 CD work_sny56posuffadh5a367n5vi4uq 252 3 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 252 4 observed observe VBN work_sny56posuffadh5a367n5vi4uq 252 5 non non JJ work_sny56posuffadh5a367n5vi4uq 252 6 - - JJ work_sny56posuffadh5a367n5vi4uq 252 7 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 252 8 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 252 9 in in IN work_sny56posuffadh5a367n5vi4uq 252 10 mouse mouse NNP work_sny56posuffadh5a367n5vi4uq 252 11 haploid haploid NNP work_sny56posuffadh5a367n5vi4uq 252 12 spermatids spermatid NNS work_sny56posuffadh5a367n5vi4uq 252 13 . . . work_sny56posuffadh5a367n5vi4uq 253 1 In in IN work_sny56posuffadh5a367n5vi4uq 253 2 this this DT work_sny56posuffadh5a367n5vi4uq 253 3 study study NN work_sny56posuffadh5a367n5vi4uq 253 4 , , , work_sny56posuffadh5a367n5vi4uq 253 5 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 253 6 determined determine VBD work_sny56posuffadh5a367n5vi4uq 253 7 the the DT work_sny56posuffadh5a367n5vi4uq 253 8 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 253 9 in in IN work_sny56posuffadh5a367n5vi4uq 253 10 tissue tissue NN work_sny56posuffadh5a367n5vi4uq 253 11 - - HYPH work_sny56posuffadh5a367n5vi4uq 253 12 cultured cultured JJ work_sny56posuffadh5a367n5vi4uq 253 13 cells cell NNS work_sny56posuffadh5a367n5vi4uq 253 14 and and CC work_sny56posuffadh5a367n5vi4uq 253 15 whole whole JJ work_sny56posuffadh5a367n5vi4uq 253 16 animals animal NNS work_sny56posuffadh5a367n5vi4uq 253 17 . . . work_sny56posuffadh5a367n5vi4uq 254 1 As as IN work_sny56posuffadh5a367n5vi4uq 254 2 summarized summarize VBN work_sny56posuffadh5a367n5vi4uq 254 3 in in IN work_sny56posuffadh5a367n5vi4uq 254 4 Table table NN work_sny56posuffadh5a367n5vi4uq 254 5 3 3 CD work_sny56posuffadh5a367n5vi4uq 254 6 , , , work_sny56posuffadh5a367n5vi4uq 254 7 in in IN work_sny56posuffadh5a367n5vi4uq 254 8 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 254 9 cells cell NNS work_sny56posuffadh5a367n5vi4uq 254 10 there there EX work_sny56posuffadh5a367n5vi4uq 254 11 is be VBZ work_sny56posuffadh5a367n5vi4uq 254 12 a a DT work_sny56posuffadh5a367n5vi4uq 254 13 mixture mixture NN work_sny56posuffadh5a367n5vi4uq 254 14 of of IN work_sny56posuffadh5a367n5vi4uq 254 15 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 254 16 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 254 17 and and CC work_sny56posuffadh5a367n5vi4uq 254 18 non non JJ work_sny56posuffadh5a367n5vi4uq 254 19 - - JJ work_sny56posuffadh5a367n5vi4uq 254 20 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 254 21 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 254 22 . . . work_sny56posuffadh5a367n5vi4uq 255 1 In in IN work_sny56posuffadh5a367n5vi4uq 255 2 zebrafish zebrafish NNP work_sny56posuffadh5a367n5vi4uq 255 3 , , , work_sny56posuffadh5a367n5vi4uq 255 4 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 255 5 embryonic embryonic JJ work_sny56posuffadh5a367n5vi4uq 255 6 stem stem NN work_sny56posuffadh5a367n5vi4uq 255 7 cells cell NNS work_sny56posuffadh5a367n5vi4uq 255 8 and and CC work_sny56posuffadh5a367n5vi4uq 255 9 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 255 10 liver liver NN work_sny56posuffadh5a367n5vi4uq 255 11 cells cell NNS work_sny56posuffadh5a367n5vi4uq 255 12 , , , work_sny56posuffadh5a367n5vi4uq 255 13 most most JJS work_sny56posuffadh5a367n5vi4uq 255 14 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 255 15 are be VBP work_sny56posuffadh5a367n5vi4uq 255 16 canon- canon- VBG work_sny56posuffadh5a367n5vi4uq 255 17 ical ical JJ work_sny56posuffadh5a367n5vi4uq 255 18 . . . work_sny56posuffadh5a367n5vi4uq 256 1 In in IN work_sny56posuffadh5a367n5vi4uq 256 2 haploid haploid JJ work_sny56posuffadh5a367n5vi4uq 256 3 spermatids spermatid NNS work_sny56posuffadh5a367n5vi4uq 256 4 , , , work_sny56posuffadh5a367n5vi4uq 256 5 none none NN work_sny56posuffadh5a367n5vi4uq 256 6 of of IN work_sny56posuffadh5a367n5vi4uq 256 7 the the DT work_sny56posuffadh5a367n5vi4uq 256 8 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 256 9 were be VBD work_sny56posuffadh5a367n5vi4uq 256 10 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 256 11 . . . work_sny56posuffadh5a367n5vi4uq 257 1 Together together RB work_sny56posuffadh5a367n5vi4uq 257 2 , , , work_sny56posuffadh5a367n5vi4uq 257 3 these these DT work_sny56posuffadh5a367n5vi4uq 257 4 results result NNS work_sny56posuffadh5a367n5vi4uq 257 5 show show VBP work_sny56posuffadh5a367n5vi4uq 257 6 that that IN work_sny56posuffadh5a367n5vi4uq 257 7 SB SB NNP work_sny56posuffadh5a367n5vi4uq 257 8 leaves leave VBZ work_sny56posuffadh5a367n5vi4uq 257 9 dif- dif- CC work_sny56posuffadh5a367n5vi4uq 257 10 ferent ferent JJ work_sny56posuffadh5a367n5vi4uq 257 11 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 257 12 in in IN work_sny56posuffadh5a367n5vi4uq 257 13 different different JJ work_sny56posuffadh5a367n5vi4uq 257 14 cell cell NN work_sny56posuffadh5a367n5vi4uq 257 15 types type NNS work_sny56posuffadh5a367n5vi4uq 257 16 and and CC work_sny56posuffadh5a367n5vi4uq 257 17 that that IN work_sny56posuffadh5a367n5vi4uq 257 18 the the DT work_sny56posuffadh5a367n5vi4uq 257 19 ability ability NN work_sny56posuffadh5a367n5vi4uq 257 20 Copyright copyright NN work_sny56posuffadh5a367n5vi4uq 257 21   CD work_sny56posuffadh5a367n5vi4uq 257 22 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 257 23 John John NNP work_sny56posuffadh5a367n5vi4uq 257 24 Wiley Wiley NNP work_sny56posuffadh5a367n5vi4uq 257 25 & & CC work_sny56posuffadh5a367n5vi4uq 257 26 Sons Sons NNPS work_sny56posuffadh5a367n5vi4uq 257 27 , , , work_sny56posuffadh5a367n5vi4uq 257 28 Ltd. Ltd. NNP work_sny56posuffadh5a367n5vi4uq 257 29 J J NNP work_sny56posuffadh5a367n5vi4uq 257 30 Gene Gene NNP work_sny56posuffadh5a367n5vi4uq 257 31 Med Med NNP work_sny56posuffadh5a367n5vi4uq 257 32 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 257 33 ; ; : work_sny56posuffadh5a367n5vi4uq 257 34 6 6 CD work_sny56posuffadh5a367n5vi4uq 257 35 : : : work_sny56posuffadh5a367n5vi4uq 257 36 574 574 CD work_sny56posuffadh5a367n5vi4uq 257 37 – – : work_sny56posuffadh5a367n5vi4uq 257 38 583 583 CD work_sny56posuffadh5a367n5vi4uq 257 39 . . . work_sny56posuffadh5a367n5vi4uq 258 1 582 582 CD work_sny56posuffadh5a367n5vi4uq 258 2 G. G. NNP work_sny56posuffadh5a367n5vi4uq 258 3 Liu Liu NNP work_sny56posuffadh5a367n5vi4uq 258 4 et et NNP work_sny56posuffadh5a367n5vi4uq 258 5 al al NNP work_sny56posuffadh5a367n5vi4uq 258 6 . . . work_sny56posuffadh5a367n5vi4uq 259 1 to to TO work_sny56posuffadh5a367n5vi4uq 259 2 revert revert VB work_sny56posuffadh5a367n5vi4uq 259 3 to to IN work_sny56posuffadh5a367n5vi4uq 259 4 wild wild JJ work_sny56posuffadh5a367n5vi4uq 259 5 type type NN work_sny56posuffadh5a367n5vi4uq 259 6 after after IN work_sny56posuffadh5a367n5vi4uq 259 7 remobilization remobilization NN work_sny56posuffadh5a367n5vi4uq 259 8 may may MD work_sny56posuffadh5a367n5vi4uq 259 9 be be VB work_sny56posuffadh5a367n5vi4uq 259 10 lim- lim- RB work_sny56posuffadh5a367n5vi4uq 259 11 ited ite VBN work_sny56posuffadh5a367n5vi4uq 259 12 by by IN work_sny56posuffadh5a367n5vi4uq 259 13 the the DT work_sny56posuffadh5a367n5vi4uq 259 14 cell cell NN work_sny56posuffadh5a367n5vi4uq 259 15 or or CC work_sny56posuffadh5a367n5vi4uq 259 16 tissue tissue NN work_sny56posuffadh5a367n5vi4uq 259 17 type type NN work_sny56posuffadh5a367n5vi4uq 259 18 . . . work_sny56posuffadh5a367n5vi4uq 260 1 In in IN work_sny56posuffadh5a367n5vi4uq 260 2 zebrafish zebrafish JJ work_sny56posuffadh5a367n5vi4uq 260 3 embryos embryo NNS work_sny56posuffadh5a367n5vi4uq 260 4 , , , work_sny56posuffadh5a367n5vi4uq 260 5 and and CC work_sny56posuffadh5a367n5vi4uq 260 6 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 260 7 embryonic embryonic JJ work_sny56posuffadh5a367n5vi4uq 260 8 stem stem NN work_sny56posuffadh5a367n5vi4uq 260 9 cells cell NNS work_sny56posuffadh5a367n5vi4uq 260 10 and and CC work_sny56posuffadh5a367n5vi4uq 260 11 cells cell NNS work_sny56posuffadh5a367n5vi4uq 260 12 of of IN work_sny56posuffadh5a367n5vi4uq 260 13 the the DT work_sny56posuffadh5a367n5vi4uq 260 14 adult adult NN work_sny56posuffadh5a367n5vi4uq 260 15 liver liver NN work_sny56posuffadh5a367n5vi4uq 260 16 , , , work_sny56posuffadh5a367n5vi4uq 260 17 90 90 CD work_sny56posuffadh5a367n5vi4uq 260 18 % % NN work_sny56posuffadh5a367n5vi4uq 260 19 of of IN work_sny56posuffadh5a367n5vi4uq 260 20 the the DT work_sny56posuffadh5a367n5vi4uq 260 21 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 260 22 add add VB work_sny56posuffadh5a367n5vi4uq 260 23 5 5 CD work_sny56posuffadh5a367n5vi4uq 260 24 bp bp NN work_sny56posuffadh5a367n5vi4uq 260 25 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 260 26 TA TA NNP work_sny56posuffadh5a367n5vi4uq 260 27 + + CC work_sny56posuffadh5a367n5vi4uq 260 28 CAG CAG NNP work_sny56posuffadh5a367n5vi4uq 260 29 or or CC work_sny56posuffadh5a367n5vi4uq 260 30 CTG CTG NNP work_sny56posuffadh5a367n5vi4uq 260 31 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 260 32 to to IN work_sny56posuffadh5a367n5vi4uq 260 33 the the DT work_sny56posuffadh5a367n5vi4uq 260 34 open open JJ work_sny56posuffadh5a367n5vi4uq 260 35 reading reading NN work_sny56posuffadh5a367n5vi4uq 260 36 frame frame NN work_sny56posuffadh5a367n5vi4uq 260 37 , , , work_sny56posuffadh5a367n5vi4uq 260 38 which which WDT work_sny56posuffadh5a367n5vi4uq 260 39 should should MD work_sny56posuffadh5a367n5vi4uq 260 40 cause cause VB work_sny56posuffadh5a367n5vi4uq 260 41 a a DT work_sny56posuffadh5a367n5vi4uq 260 42 frame frame NN work_sny56posuffadh5a367n5vi4uq 260 43 shift shift NN work_sny56posuffadh5a367n5vi4uq 260 44 . . . work_sny56posuffadh5a367n5vi4uq 261 1 In in IN work_sny56posuffadh5a367n5vi4uq 261 2 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 261 3 haploid haploid NN work_sny56posuffadh5a367n5vi4uq 261 4 spermatids spermatid NNS work_sny56posuffadh5a367n5vi4uq 261 5 , , , work_sny56posuffadh5a367n5vi4uq 261 6 over over IN work_sny56posuffadh5a367n5vi4uq 261 7 40 40 CD work_sny56posuffadh5a367n5vi4uq 261 8 % % NN work_sny56posuffadh5a367n5vi4uq 261 9 of of IN work_sny56posuffadh5a367n5vi4uq 261 10 the the DT work_sny56posuffadh5a367n5vi4uq 261 11 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 261 12 add add VB work_sny56posuffadh5a367n5vi4uq 261 13 only only RB work_sny56posuffadh5a367n5vi4uq 261 14 3 3 CD work_sny56posuffadh5a367n5vi4uq 261 15 bp bp NN work_sny56posuffadh5a367n5vi4uq 261 16 , , , work_sny56posuffadh5a367n5vi4uq 261 17 which which WDT work_sny56posuffadh5a367n5vi4uq 261 18 would would MD work_sny56posuffadh5a367n5vi4uq 261 19 allow allow VB work_sny56posuffadh5a367n5vi4uq 261 20 reversion reversion NN work_sny56posuffadh5a367n5vi4uq 261 21 to to IN work_sny56posuffadh5a367n5vi4uq 261 22 the the DT work_sny56posuffadh5a367n5vi4uq 261 23 wild- wild- NN work_sny56posuffadh5a367n5vi4uq 261 24 type type NN work_sny56posuffadh5a367n5vi4uq 261 25 phenotype phenotype NN work_sny56posuffadh5a367n5vi4uq 261 26 . . . work_sny56posuffadh5a367n5vi4uq 262 1 Thus thus RB work_sny56posuffadh5a367n5vi4uq 262 2 , , , work_sny56posuffadh5a367n5vi4uq 262 3 for for IN work_sny56posuffadh5a367n5vi4uq 262 4 experimental experimental JJ work_sny56posuffadh5a367n5vi4uq 262 5 studies study NNS work_sny56posuffadh5a367n5vi4uq 262 6 , , , work_sny56posuffadh5a367n5vi4uq 262 7 reversion reversion NN work_sny56posuffadh5a367n5vi4uq 262 8 to to IN work_sny56posuffadh5a367n5vi4uq 262 9 wild wild JJ work_sny56posuffadh5a367n5vi4uq 262 10 type type NN work_sny56posuffadh5a367n5vi4uq 262 11 would would MD work_sny56posuffadh5a367n5vi4uq 262 12 be be VB work_sny56posuffadh5a367n5vi4uq 262 13 rare rare JJ work_sny56posuffadh5a367n5vi4uq 262 14 when when WRB work_sny56posuffadh5a367n5vi4uq 262 15 using use VBG work_sny56posuffadh5a367n5vi4uq 262 16 SB SB NNP work_sny56posuffadh5a367n5vi4uq 262 17 in in IN work_sny56posuffadh5a367n5vi4uq 262 18 zebrafish zebrafish NNP work_sny56posuffadh5a367n5vi4uq 262 19 embryos embryos NNP work_sny56posuffadh5a367n5vi4uq 262 20 , , , work_sny56posuffadh5a367n5vi4uq 262 21 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 262 22 embryonic embryonic JJ work_sny56posuffadh5a367n5vi4uq 262 23 stem stem NN work_sny56posuffadh5a367n5vi4uq 262 24 cells cell NNS work_sny56posuffadh5a367n5vi4uq 262 25 , , , work_sny56posuffadh5a367n5vi4uq 262 26 and and CC work_sny56posuffadh5a367n5vi4uq 262 27 tissues tissue NNS work_sny56posuffadh5a367n5vi4uq 262 28 in in IN work_sny56posuffadh5a367n5vi4uq 262 29 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 262 30 . . . work_sny56posuffadh5a367n5vi4uq 263 1 The the DT work_sny56posuffadh5a367n5vi4uq 263 2 footprint footprint NN work_sny56posuffadh5a367n5vi4uq 263 3 data datum NNS work_sny56posuffadh5a367n5vi4uq 263 4 also also RB work_sny56posuffadh5a367n5vi4uq 263 5 showed show VBD work_sny56posuffadh5a367n5vi4uq 263 6 that that IN work_sny56posuffadh5a367n5vi4uq 263 7 when when WRB work_sny56posuffadh5a367n5vi4uq 263 8 GG GG NNP work_sny56posuffadh5a367n5vi4uq 263 9 replaced replace VBD work_sny56posuffadh5a367n5vi4uq 263 10 the the DT work_sny56posuffadh5a367n5vi4uq 263 11 TA TA NNP work_sny56posuffadh5a367n5vi4uq 263 12 outside outside IN work_sny56posuffadh5a367n5vi4uq 263 13 the the DT work_sny56posuffadh5a367n5vi4uq 263 14 outer outer JJ work_sny56posuffadh5a367n5vi4uq 263 15 DR DR NNP work_sny56posuffadh5a367n5vi4uq 263 16 , , , work_sny56posuffadh5a367n5vi4uq 263 17 the the DT work_sny56posuffadh5a367n5vi4uq 263 18 substitution substitution NN work_sny56posuffadh5a367n5vi4uq 263 19 remained remain VBD work_sny56posuffadh5a367n5vi4uq 263 20 in in IN work_sny56posuffadh5a367n5vi4uq 263 21 the the DT work_sny56posuffadh5a367n5vi4uq 263 22 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 263 23 . . . work_sny56posuffadh5a367n5vi4uq 264 1 This this DT work_sny56posuffadh5a367n5vi4uq 264 2 supports support VBZ work_sny56posuffadh5a367n5vi4uq 264 3 the the DT work_sny56posuffadh5a367n5vi4uq 264 4 current current JJ work_sny56posuffadh5a367n5vi4uq 264 5 model model NN work_sny56posuffadh5a367n5vi4uq 264 6 that that WDT work_sny56posuffadh5a367n5vi4uq 264 7 the the DT work_sny56posuffadh5a367n5vi4uq 264 8 TA TA NNP work_sny56posuffadh5a367n5vi4uq 264 9 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 264 10 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 264 11 the the DT work_sny56posuffadh5a367n5vi4uq 264 12 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 264 13 are be VBP work_sny56posuffadh5a367n5vi4uq 264 14 not not RB work_sny56posuffadh5a367n5vi4uq 264 15 part part NN work_sny56posuffadh5a367n5vi4uq 264 16 of of IN work_sny56posuffadh5a367n5vi4uq 264 17 the the DT work_sny56posuffadh5a367n5vi4uq 264 18 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 264 19 and and CC work_sny56posuffadh5a367n5vi4uq 264 20 are be VBP work_sny56posuffadh5a367n5vi4uq 264 21 not not RB work_sny56posuffadh5a367n5vi4uq 264 22 carried carry VBN work_sny56posuffadh5a367n5vi4uq 264 23 over over RP work_sny56posuffadh5a367n5vi4uq 264 24 into into IN work_sny56posuffadh5a367n5vi4uq 264 25 a a DT work_sny56posuffadh5a367n5vi4uq 264 26 target target NN work_sny56posuffadh5a367n5vi4uq 264 27 site site NN work_sny56posuffadh5a367n5vi4uq 264 28 . . . work_sny56posuffadh5a367n5vi4uq 265 1 Double double JJ work_sny56posuffadh5a367n5vi4uq 265 2 - - HYPH work_sny56posuffadh5a367n5vi4uq 265 3 strand strand NN work_sny56posuffadh5a367n5vi4uq 265 4 breaks break NNS work_sny56posuffadh5a367n5vi4uq 265 5 generated generate VBN work_sny56posuffadh5a367n5vi4uq 265 6 by by IN work_sny56posuffadh5a367n5vi4uq 265 7 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 265 8 excision excision NN work_sny56posuffadh5a367n5vi4uq 265 9 are be VBP work_sny56posuffadh5a367n5vi4uq 265 10 thought think VBN work_sny56posuffadh5a367n5vi4uq 265 11 to to TO work_sny56posuffadh5a367n5vi4uq 265 12 be be VB work_sny56posuffadh5a367n5vi4uq 265 13 repaired repair VBN work_sny56posuffadh5a367n5vi4uq 265 14 by by IN work_sny56posuffadh5a367n5vi4uq 265 15 a a DT work_sny56posuffadh5a367n5vi4uq 265 16 process process NN work_sny56posuffadh5a367n5vi4uq 265 17 called call VBN work_sny56posuffadh5a367n5vi4uq 265 18 non- non- NNP work_sny56posuffadh5a367n5vi4uq 265 19 homologous homologous JJ work_sny56posuffadh5a367n5vi4uq 265 20 end end NN work_sny56posuffadh5a367n5vi4uq 265 21 - - HYPH work_sny56posuffadh5a367n5vi4uq 265 22 joining join VBG work_sny56posuffadh5a367n5vi4uq 265 23 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 265 24 4 4 CD work_sny56posuffadh5a367n5vi4uq 265 25 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 265 26 . . . work_sny56posuffadh5a367n5vi4uq 266 1 In in IN work_sny56posuffadh5a367n5vi4uq 266 2 vertebrates vertebrate NNS work_sny56posuffadh5a367n5vi4uq 266 3 , , , work_sny56posuffadh5a367n5vi4uq 266 4 this this DT work_sny56posuffadh5a367n5vi4uq 266 5 process process NN work_sny56posuffadh5a367n5vi4uq 266 6 is be VBZ work_sny56posuffadh5a367n5vi4uq 266 7 catalyzed catalyze VBN work_sny56posuffadh5a367n5vi4uq 266 8 by by IN work_sny56posuffadh5a367n5vi4uq 266 9 a a DT work_sny56posuffadh5a367n5vi4uq 266 10 group group NN work_sny56posuffadh5a367n5vi4uq 266 11 of of IN work_sny56posuffadh5a367n5vi4uq 266 12 enzymes enzyme NNS work_sny56posuffadh5a367n5vi4uq 266 13 including include VBG work_sny56posuffadh5a367n5vi4uq 266 14 Ku70 Ku70 NNP work_sny56posuffadh5a367n5vi4uq 266 15 and and CC work_sny56posuffadh5a367n5vi4uq 266 16 Ku80 Ku80 NNP work_sny56posuffadh5a367n5vi4uq 266 17 end end VB work_sny56posuffadh5a367n5vi4uq 266 18 - - HYPH work_sny56posuffadh5a367n5vi4uq 266 19 binding bind VBG work_sny56posuffadh5a367n5vi4uq 266 20 factors factor NNS work_sny56posuffadh5a367n5vi4uq 266 21 , , , work_sny56posuffadh5a367n5vi4uq 266 22 the the DT work_sny56posuffadh5a367n5vi4uq 266 23 catalytic catalytic JJ work_sny56posuffadh5a367n5vi4uq 266 24 subunit subunit NN work_sny56posuffadh5a367n5vi4uq 266 25 of of IN work_sny56posuffadh5a367n5vi4uq 266 26 DNA- DNA- NNP work_sny56posuffadh5a367n5vi4uq 266 27 dependent dependent JJ work_sny56posuffadh5a367n5vi4uq 266 28 protein protein NN work_sny56posuffadh5a367n5vi4uq 266 29 kinase kinase NN work_sny56posuffadh5a367n5vi4uq 266 30 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 266 31 DNA DNA NNP work_sny56posuffadh5a367n5vi4uq 266 32 - - HYPH work_sny56posuffadh5a367n5vi4uq 266 33 PK PK NNP work_sny56posuffadh5a367n5vi4uq 266 34 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 266 35 , , , work_sny56posuffadh5a367n5vi4uq 266 36 and and CC work_sny56posuffadh5a367n5vi4uq 266 37 the the DT work_sny56posuffadh5a367n5vi4uq 266 38 XRCC4 XRCC4 NNP work_sny56posuffadh5a367n5vi4uq 266 39 / / SYM work_sny56posuffadh5a367n5vi4uq 266 40 DNA DNA NNP work_sny56posuffadh5a367n5vi4uq 266 41 ligase ligase NN work_sny56posuffadh5a367n5vi4uq 266 42 IV IV NNP work_sny56posuffadh5a367n5vi4uq 266 43 heteromeric heteromeric NN work_sny56posuffadh5a367n5vi4uq 266 44 complex complex NN work_sny56posuffadh5a367n5vi4uq 266 45 . . . work_sny56posuffadh5a367n5vi4uq 267 1 Mutation mutation NN work_sny56posuffadh5a367n5vi4uq 267 2 studies study NNS work_sny56posuffadh5a367n5vi4uq 267 3 in in IN work_sny56posuffadh5a367n5vi4uq 267 4 yeast yeast NN work_sny56posuffadh5a367n5vi4uq 267 5 have have VBP work_sny56posuffadh5a367n5vi4uq 267 6 shown show VBN work_sny56posuffadh5a367n5vi4uq 267 7 that that DT work_sny56posuffadh5a367n5vi4uq 267 8 loss loss NN work_sny56posuffadh5a367n5vi4uq 267 9 of of IN work_sny56posuffadh5a367n5vi4uq 267 10 different different JJ work_sny56posuffadh5a367n5vi4uq 267 11 subsets subset NNS work_sny56posuffadh5a367n5vi4uq 267 12 of of IN work_sny56posuffadh5a367n5vi4uq 267 13 these these DT work_sny56posuffadh5a367n5vi4uq 267 14 enzymes enzyme NNS work_sny56posuffadh5a367n5vi4uq 267 15 leads lead VBZ work_sny56posuffadh5a367n5vi4uq 267 16 to to IN work_sny56posuffadh5a367n5vi4uq 267 17 different different JJ work_sny56posuffadh5a367n5vi4uq 267 18 repair repair NN work_sny56posuffadh5a367n5vi4uq 267 19 products product NNS work_sny56posuffadh5a367n5vi4uq 267 20 , , , work_sny56posuffadh5a367n5vi4uq 267 21 including include VBG work_sny56posuffadh5a367n5vi4uq 267 22 accurate accurate JJ work_sny56posuffadh5a367n5vi4uq 267 23 repair repair NN work_sny56posuffadh5a367n5vi4uq 267 24 , , , work_sny56posuffadh5a367n5vi4uq 267 25 inaccurate inaccurate JJ work_sny56posuffadh5a367n5vi4uq 267 26 repair repair NN work_sny56posuffadh5a367n5vi4uq 267 27 , , , work_sny56posuffadh5a367n5vi4uq 267 28 and and CC work_sny56posuffadh5a367n5vi4uq 267 29 a a DT work_sny56posuffadh5a367n5vi4uq 267 30 mixture mixture NN work_sny56posuffadh5a367n5vi4uq 267 31 of of IN work_sny56posuffadh5a367n5vi4uq 267 32 accurate accurate JJ work_sny56posuffadh5a367n5vi4uq 267 33 and and CC work_sny56posuffadh5a367n5vi4uq 267 34 inaccurate inaccurate JJ work_sny56posuffadh5a367n5vi4uq 267 35 repair repair NN work_sny56posuffadh5a367n5vi4uq 267 36 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 267 37 24 24 CD work_sny56posuffadh5a367n5vi4uq 267 38 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 267 39 . . . work_sny56posuffadh5a367n5vi4uq 268 1 These these DT work_sny56posuffadh5a367n5vi4uq 268 2 results result NNS work_sny56posuffadh5a367n5vi4uq 268 3 resemble resemble VBP work_sny56posuffadh5a367n5vi4uq 268 4 the the DT work_sny56posuffadh5a367n5vi4uq 268 5 different different JJ work_sny56posuffadh5a367n5vi4uq 268 6 footprint footprint NN work_sny56posuffadh5a367n5vi4uq 268 7 patterns pattern NNS work_sny56posuffadh5a367n5vi4uq 268 8 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 268 9 observed observe VBD work_sny56posuffadh5a367n5vi4uq 268 10 in in IN work_sny56posuffadh5a367n5vi4uq 268 11 different different JJ work_sny56posuffadh5a367n5vi4uq 268 12 cell cell NN work_sny56posuffadh5a367n5vi4uq 268 13 types type NNS work_sny56posuffadh5a367n5vi4uq 268 14 – – : work_sny56posuffadh5a367n5vi4uq 268 15 mostly mostly RB work_sny56posuffadh5a367n5vi4uq 268 16 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 268 17 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 268 18 in in IN work_sny56posuffadh5a367n5vi4uq 268 19 zebrafish zebrafish JJ work_sny56posuffadh5a367n5vi4uq 268 20 cells cell NNS work_sny56posuffadh5a367n5vi4uq 268 21 as as RB work_sny56posuffadh5a367n5vi4uq 268 22 well well RB work_sny56posuffadh5a367n5vi4uq 268 23 as as IN work_sny56posuffadh5a367n5vi4uq 268 24 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 268 25 embryonic embryonic JJ work_sny56posuffadh5a367n5vi4uq 268 26 stem stem NN work_sny56posuffadh5a367n5vi4uq 268 27 cells cell NNS work_sny56posuffadh5a367n5vi4uq 268 28 and and CC work_sny56posuffadh5a367n5vi4uq 268 29 liver liver NN work_sny56posuffadh5a367n5vi4uq 268 30 cells cell NNS work_sny56posuffadh5a367n5vi4uq 268 31 , , , work_sny56posuffadh5a367n5vi4uq 268 32 non- non- NNP work_sny56posuffadh5a367n5vi4uq 268 33 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 268 34 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 268 35 in in IN work_sny56posuffadh5a367n5vi4uq 268 36 mouse mouse NNP work_sny56posuffadh5a367n5vi4uq 268 37 haploid haploid NN work_sny56posuffadh5a367n5vi4uq 268 38 spermatids spermatid NNS work_sny56posuffadh5a367n5vi4uq 268 39 , , , work_sny56posuffadh5a367n5vi4uq 268 40 and and CC work_sny56posuffadh5a367n5vi4uq 268 41 a a DT work_sny56posuffadh5a367n5vi4uq 268 42 mixture mixture NN work_sny56posuffadh5a367n5vi4uq 268 43 of of IN work_sny56posuffadh5a367n5vi4uq 268 44 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 268 45 and and CC work_sny56posuffadh5a367n5vi4uq 268 46 non non JJ work_sny56posuffadh5a367n5vi4uq 268 47 - - JJ work_sny56posuffadh5a367n5vi4uq 268 48 canonical canonical JJ work_sny56posuffadh5a367n5vi4uq 268 49 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 268 50 in in IN work_sny56posuffadh5a367n5vi4uq 268 51 HeLa HeLa NNP work_sny56posuffadh5a367n5vi4uq 268 52 cells cell NNS work_sny56posuffadh5a367n5vi4uq 268 53 . . . work_sny56posuffadh5a367n5vi4uq 269 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 269 2 suspect suspect VBP work_sny56posuffadh5a367n5vi4uq 269 3 that that IN work_sny56posuffadh5a367n5vi4uq 269 4 the the DT work_sny56posuffadh5a367n5vi4uq 269 5 DNA DNA NNP work_sny56posuffadh5a367n5vi4uq 269 6 repair repair NN work_sny56posuffadh5a367n5vi4uq 269 7 machineries machinery NNS work_sny56posuffadh5a367n5vi4uq 269 8 differ differ VBP work_sny56posuffadh5a367n5vi4uq 269 9 in in IN work_sny56posuffadh5a367n5vi4uq 269 10 some some DT work_sny56posuffadh5a367n5vi4uq 269 11 way way NN work_sny56posuffadh5a367n5vi4uq 269 12 that that WDT work_sny56posuffadh5a367n5vi4uq 269 13 leaves leave VBZ work_sny56posuffadh5a367n5vi4uq 269 14 characteristic characteristic JJ work_sny56posuffadh5a367n5vi4uq 269 15 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 269 16 for for IN work_sny56posuffadh5a367n5vi4uq 269 17 each each DT work_sny56posuffadh5a367n5vi4uq 269 18 cell cell NN work_sny56posuffadh5a367n5vi4uq 269 19 type type NN work_sny56posuffadh5a367n5vi4uq 269 20 . . . work_sny56posuffadh5a367n5vi4uq 270 1 This this DT work_sny56posuffadh5a367n5vi4uq 270 2 hypothesis hypothesis NN work_sny56posuffadh5a367n5vi4uq 270 3 could could MD work_sny56posuffadh5a367n5vi4uq 270 4 be be VB work_sny56posuffadh5a367n5vi4uq 270 5 further further RB work_sny56posuffadh5a367n5vi4uq 270 6 investigated investigate VBN work_sny56posuffadh5a367n5vi4uq 270 7 by by IN work_sny56posuffadh5a367n5vi4uq 270 8 examining examine VBG work_sny56posuffadh5a367n5vi4uq 270 9 SB SB NNP work_sny56posuffadh5a367n5vi4uq 270 10 footprints footprint NNS work_sny56posuffadh5a367n5vi4uq 270 11 in in IN work_sny56posuffadh5a367n5vi4uq 270 12 cell cell NN work_sny56posuffadh5a367n5vi4uq 270 13 lines line NNS work_sny56posuffadh5a367n5vi4uq 270 14 with with IN work_sny56posuffadh5a367n5vi4uq 270 15 known know VBN work_sny56posuffadh5a367n5vi4uq 270 16 repair repair NN work_sny56posuffadh5a367n5vi4uq 270 17 defects defect NNS work_sny56posuffadh5a367n5vi4uq 270 18 . . . work_sny56posuffadh5a367n5vi4uq 271 1 The the DT work_sny56posuffadh5a367n5vi4uq 271 2 excision excision NN work_sny56posuffadh5a367n5vi4uq 271 3 step step NN work_sny56posuffadh5a367n5vi4uq 271 4 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 271 5 studied study VBD work_sny56posuffadh5a367n5vi4uq 271 6 here here RB work_sny56posuffadh5a367n5vi4uq 271 7 is be VBZ work_sny56posuffadh5a367n5vi4uq 271 8 one one CD work_sny56posuffadh5a367n5vi4uq 271 9 of of IN work_sny56posuffadh5a367n5vi4uq 271 10 the the DT work_sny56posuffadh5a367n5vi4uq 271 11 major major JJ work_sny56posuffadh5a367n5vi4uq 271 12 steps step NNS work_sny56posuffadh5a367n5vi4uq 271 13 involved involve VBN work_sny56posuffadh5a367n5vi4uq 271 14 in in IN work_sny56posuffadh5a367n5vi4uq 271 15 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 271 16 . . . work_sny56posuffadh5a367n5vi4uq 272 1 Studies study NNS work_sny56posuffadh5a367n5vi4uq 272 2 from from IN work_sny56posuffadh5a367n5vi4uq 272 3 other other JJ work_sny56posuffadh5a367n5vi4uq 272 4 DNA dna NN work_sny56posuffadh5a367n5vi4uq 272 5 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 272 6 such such JJ work_sny56posuffadh5a367n5vi4uq 272 7 as as IN work_sny56posuffadh5a367n5vi4uq 272 8 Tn10 Tn10 NNP work_sny56posuffadh5a367n5vi4uq 272 9 , , , work_sny56posuffadh5a367n5vi4uq 272 10 Tn5 tn5 CD work_sny56posuffadh5a367n5vi4uq 272 11 and and CC work_sny56posuffadh5a367n5vi4uq 272 12 Mu Mu NNP work_sny56posuffadh5a367n5vi4uq 272 13 have have VBP work_sny56posuffadh5a367n5vi4uq 272 14 identified identify VBN work_sny56posuffadh5a367n5vi4uq 272 15 the the DT work_sny56posuffadh5a367n5vi4uq 272 16 detailed detailed JJ work_sny56posuffadh5a367n5vi4uq 272 17 steps step NNS work_sny56posuffadh5a367n5vi4uq 272 18 of of IN work_sny56posuffadh5a367n5vi4uq 272 19 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 272 20 . . . work_sny56posuffadh5a367n5vi4uq 273 1 Specifically specifically RB work_sny56posuffadh5a367n5vi4uq 273 2 , , , work_sny56posuffadh5a367n5vi4uq 273 3 excision excision NN work_sny56posuffadh5a367n5vi4uq 273 4 of of IN work_sny56posuffadh5a367n5vi4uq 273 5 the the DT work_sny56posuffadh5a367n5vi4uq 273 6 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 273 7 consists consist VBZ work_sny56posuffadh5a367n5vi4uq 273 8 of of IN work_sny56posuffadh5a367n5vi4uq 273 9 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 273 10 - - HYPH work_sny56posuffadh5a367n5vi4uq 273 11 binding bind VBG work_sny56posuffadh5a367n5vi4uq 273 12 to to IN work_sny56posuffadh5a367n5vi4uq 273 13 the the DT work_sny56posuffadh5a367n5vi4uq 273 14 terminal terminal JJ work_sny56posuffadh5a367n5vi4uq 273 15 repeats repeat NNS work_sny56posuffadh5a367n5vi4uq 273 16 followed follow VBN work_sny56posuffadh5a367n5vi4uq 273 17 by by IN work_sny56posuffadh5a367n5vi4uq 273 18 formation formation NN work_sny56posuffadh5a367n5vi4uq 273 19 of of IN work_sny56posuffadh5a367n5vi4uq 273 20 a a DT work_sny56posuffadh5a367n5vi4uq 273 21 synaptic synaptic JJ work_sny56posuffadh5a367n5vi4uq 273 22 complex complex NN work_sny56posuffadh5a367n5vi4uq 273 23 comprising comprise VBG work_sny56posuffadh5a367n5vi4uq 273 24 the the DT work_sny56posuffadh5a367n5vi4uq 273 25 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 273 26 – – : work_sny56posuffadh5a367n5vi4uq 273 27 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 273 28 . . . work_sny56posuffadh5a367n5vi4uq 274 1 For for IN work_sny56posuffadh5a367n5vi4uq 274 2 the the DT work_sny56posuffadh5a367n5vi4uq 274 3 Mu Mu NNP work_sny56posuffadh5a367n5vi4uq 274 4 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 274 5 , , , work_sny56posuffadh5a367n5vi4uq 274 6 this this DT work_sny56posuffadh5a367n5vi4uq 274 7 step step NN work_sny56posuffadh5a367n5vi4uq 274 8 is be VBZ work_sny56posuffadh5a367n5vi4uq 274 9 called call VBN work_sny56posuffadh5a367n5vi4uq 274 10 transpososome transpososome NN work_sny56posuffadh5a367n5vi4uq 274 11 assembly assembly NN work_sny56posuffadh5a367n5vi4uq 274 12 . . . work_sny56posuffadh5a367n5vi4uq 275 1 The the DT work_sny56posuffadh5a367n5vi4uq 275 2 cut cut VBN work_sny56posuffadh5a367n5vi4uq 275 3 - - HYPH work_sny56posuffadh5a367n5vi4uq 275 4 and and CC work_sny56posuffadh5a367n5vi4uq 275 5 - - HYPH work_sny56posuffadh5a367n5vi4uq 275 6 paste paste NN work_sny56posuffadh5a367n5vi4uq 275 7 reaction reaction NN work_sny56posuffadh5a367n5vi4uq 275 8 then then RB work_sny56posuffadh5a367n5vi4uq 275 9 occurs occur VBZ work_sny56posuffadh5a367n5vi4uq 275 10 with with IN work_sny56posuffadh5a367n5vi4uq 275 11 first first JJ work_sny56posuffadh5a367n5vi4uq 275 12 - - HYPH work_sny56posuffadh5a367n5vi4uq 275 13 strand strand NN work_sny56posuffadh5a367n5vi4uq 275 14 nicking nicking NN work_sny56posuffadh5a367n5vi4uq 275 15 , , , work_sny56posuffadh5a367n5vi4uq 275 16 hairpin hairpin NN work_sny56posuffadh5a367n5vi4uq 275 17 formation formation NN work_sny56posuffadh5a367n5vi4uq 275 18 and and CC work_sny56posuffadh5a367n5vi4uq 275 19 hairpin hairpin NN work_sny56posuffadh5a367n5vi4uq 275 20 resolution resolution NN work_sny56posuffadh5a367n5vi4uq 275 21 , , , work_sny56posuffadh5a367n5vi4uq 275 22 which which WDT work_sny56posuffadh5a367n5vi4uq 275 23 releases release VBZ work_sny56posuffadh5a367n5vi4uq 275 24 the the DT work_sny56posuffadh5a367n5vi4uq 275 25 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 275 26 from from IN work_sny56posuffadh5a367n5vi4uq 275 27 the the DT work_sny56posuffadh5a367n5vi4uq 275 28 donor donor NN work_sny56posuffadh5a367n5vi4uq 275 29 site site NN work_sny56posuffadh5a367n5vi4uq 275 30 , , , work_sny56posuffadh5a367n5vi4uq 275 31 and and CC work_sny56posuffadh5a367n5vi4uq 275 32 marks mark VBZ work_sny56posuffadh5a367n5vi4uq 275 33 the the DT work_sny56posuffadh5a367n5vi4uq 275 34 end end NN work_sny56posuffadh5a367n5vi4uq 275 35 of of IN work_sny56posuffadh5a367n5vi4uq 275 36 the the DT work_sny56posuffadh5a367n5vi4uq 275 37 excision excision NN work_sny56posuffadh5a367n5vi4uq 275 38 step step NN work_sny56posuffadh5a367n5vi4uq 275 39 . . . work_sny56posuffadh5a367n5vi4uq 276 1 Biochemical biochemical JJ work_sny56posuffadh5a367n5vi4uq 276 2 studies study NNS work_sny56posuffadh5a367n5vi4uq 276 3 in in IN work_sny56posuffadh5a367n5vi4uq 276 4 these these DT work_sny56posuffadh5a367n5vi4uq 276 5 systems system NNS work_sny56posuffadh5a367n5vi4uq 276 6 have have VBP work_sny56posuffadh5a367n5vi4uq 276 7 shown show VBN work_sny56posuffadh5a367n5vi4uq 276 8 that that IN work_sny56posuffadh5a367n5vi4uq 276 9 the the DT work_sny56posuffadh5a367n5vi4uq 276 10 terminal terminal JJ work_sny56posuffadh5a367n5vi4uq 276 11 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 276 12 at at IN work_sny56posuffadh5a367n5vi4uq 276 13 the the DT work_sny56posuffadh5a367n5vi4uq 276 14 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 276 15 - - HYPH work_sny56posuffadh5a367n5vi4uq 276 16 donor donor NN work_sny56posuffadh5a367n5vi4uq 276 17 junction junction NN work_sny56posuffadh5a367n5vi4uq 276 18 are be VBP work_sny56posuffadh5a367n5vi4uq 276 19 involved involve VBN work_sny56posuffadh5a367n5vi4uq 276 20 in in IN work_sny56posuffadh5a367n5vi4uq 276 21 steps step NNS work_sny56posuffadh5a367n5vi4uq 276 22 subsequent subsequent JJ work_sny56posuffadh5a367n5vi4uq 276 23 to to IN work_sny56posuffadh5a367n5vi4uq 276 24 DNA dna NN work_sny56posuffadh5a367n5vi4uq 276 25 binding bind VBG work_sny56posuffadh5a367n5vi4uq 276 26 but but CC work_sny56posuffadh5a367n5vi4uq 276 27 before before IN work_sny56posuffadh5a367n5vi4uq 276 28 excision excision NN work_sny56posuffadh5a367n5vi4uq 276 29 . . . work_sny56posuffadh5a367n5vi4uq 277 1 In in IN work_sny56posuffadh5a367n5vi4uq 277 2 Mu Mu NNP work_sny56posuffadh5a367n5vi4uq 277 3 , , , work_sny56posuffadh5a367n5vi4uq 277 4 the the DT work_sny56posuffadh5a367n5vi4uq 277 5 terminal terminal JJ work_sny56posuffadh5a367n5vi4uq 277 6 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 277 7 at at IN work_sny56posuffadh5a367n5vi4uq 277 8 the the DT work_sny56posuffadh5a367n5vi4uq 277 9 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 277 10 - - HYPH work_sny56posuffadh5a367n5vi4uq 277 11 junction junction NNP work_sny56posuffadh5a367n5vi4uq 277 12 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 277 13 are be VBP work_sny56posuffadh5a367n5vi4uq 277 14 involved involve VBN work_sny56posuffadh5a367n5vi4uq 277 15 in in IN work_sny56posuffadh5a367n5vi4uq 277 16 transpososome transpososome NN work_sny56posuffadh5a367n5vi4uq 277 17 assembly assembly NN work_sny56posuffadh5a367n5vi4uq 277 18 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 277 19 26,27 26,27 CD work_sny56posuffadh5a367n5vi4uq 277 20 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 277 21 . . . work_sny56posuffadh5a367n5vi4uq 278 1 In in IN work_sny56posuffadh5a367n5vi4uq 278 2 Tn5 tn5 CD work_sny56posuffadh5a367n5vi4uq 278 3 , , , work_sny56posuffadh5a367n5vi4uq 278 4 the the DT work_sny56posuffadh5a367n5vi4uq 278 5 end end NN work_sny56posuffadh5a367n5vi4uq 278 6 sequences sequence NNS work_sny56posuffadh5a367n5vi4uq 278 7 are be VBP work_sny56posuffadh5a367n5vi4uq 278 8 specifically specifically RB work_sny56posuffadh5a367n5vi4uq 278 9 required require VBN work_sny56posuffadh5a367n5vi4uq 278 10 for for IN work_sny56posuffadh5a367n5vi4uq 278 11 synaptic synaptic JJ work_sny56posuffadh5a367n5vi4uq 278 12 complex complex JJ work_sny56posuffadh5a367n5vi4uq 278 13 formation formation NN work_sny56posuffadh5a367n5vi4uq 278 14 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 278 15 28 28 CD work_sny56posuffadh5a367n5vi4uq 278 16 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 278 17 . . . work_sny56posuffadh5a367n5vi4uq 279 1 In in IN work_sny56posuffadh5a367n5vi4uq 279 2 Tn10 Tn10 NNP work_sny56posuffadh5a367n5vi4uq 279 3 , , , work_sny56posuffadh5a367n5vi4uq 279 4 mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 279 5 in in IN work_sny56posuffadh5a367n5vi4uq 279 6 these these DT work_sny56posuffadh5a367n5vi4uq 279 7 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 279 8 prevent prevent VBP work_sny56posuffadh5a367n5vi4uq 279 9 hairpin hairpin NN work_sny56posuffadh5a367n5vi4uq 279 10 formation formation NN work_sny56posuffadh5a367n5vi4uq 279 11 and and CC work_sny56posuffadh5a367n5vi4uq 279 12 strand strand NN work_sny56posuffadh5a367n5vi4uq 279 13 transfer transfer NN work_sny56posuffadh5a367n5vi4uq 279 14 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 279 15 25 25 CD work_sny56posuffadh5a367n5vi4uq 279 16 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 279 17 . . . work_sny56posuffadh5a367n5vi4uq 280 1 In in IN work_sny56posuffadh5a367n5vi4uq 280 2 SB SB NNP work_sny56posuffadh5a367n5vi4uq 280 3 - - HYPH work_sny56posuffadh5a367n5vi4uq 280 4 mediated mediate VBN work_sny56posuffadh5a367n5vi4uq 280 5 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 280 6 , , , work_sny56posuffadh5a367n5vi4uq 280 7 mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 280 8 in in IN work_sny56posuffadh5a367n5vi4uq 280 9 terminal terminal JJ work_sny56posuffadh5a367n5vi4uq 280 10 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 280 11 do do VBP work_sny56posuffadh5a367n5vi4uq 280 12 not not RB work_sny56posuffadh5a367n5vi4uq 280 13 affect affect VB work_sny56posuffadh5a367n5vi4uq 280 14 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 280 15 binding bind VBG work_sny56posuffadh5a367n5vi4uq 280 16 , , , work_sny56posuffadh5a367n5vi4uq 280 17 but but CC work_sny56posuffadh5a367n5vi4uq 280 18 do do VB work_sny56posuffadh5a367n5vi4uq 280 19 affect affect VB work_sny56posuffadh5a367n5vi4uq 280 20 excision excision NN work_sny56posuffadh5a367n5vi4uq 280 21 activity activity NN work_sny56posuffadh5a367n5vi4uq 280 22 , , , work_sny56posuffadh5a367n5vi4uq 280 23 indicating indicate VBG work_sny56posuffadh5a367n5vi4uq 280 24 they -PRON- PRP work_sny56posuffadh5a367n5vi4uq 280 25 may may MD work_sny56posuffadh5a367n5vi4uq 280 26 have have VB work_sny56posuffadh5a367n5vi4uq 280 27 a a DT work_sny56posuffadh5a367n5vi4uq 280 28 similar similar JJ work_sny56posuffadh5a367n5vi4uq 280 29 function function NN work_sny56posuffadh5a367n5vi4uq 280 30 subsequent subsequent JJ work_sny56posuffadh5a367n5vi4uq 280 31 to to IN work_sny56posuffadh5a367n5vi4uq 280 32 DNA dna NN work_sny56posuffadh5a367n5vi4uq 280 33 binding bind VBG work_sny56posuffadh5a367n5vi4uq 280 34 but but CC work_sny56posuffadh5a367n5vi4uq 280 35 before before IN work_sny56posuffadh5a367n5vi4uq 280 36 excision excision NN work_sny56posuffadh5a367n5vi4uq 280 37 as as IN work_sny56posuffadh5a367n5vi4uq 280 38 with with IN work_sny56posuffadh5a367n5vi4uq 280 39 other other JJ work_sny56posuffadh5a367n5vi4uq 280 40 DNA dna NN work_sny56posuffadh5a367n5vi4uq 280 41 transposons transposon NNS work_sny56posuffadh5a367n5vi4uq 280 42 . . . work_sny56posuffadh5a367n5vi4uq 281 1 Our -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 281 2 study study NN work_sny56posuffadh5a367n5vi4uq 281 3 of of IN work_sny56posuffadh5a367n5vi4uq 281 4 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 281 5 bordering border VBG work_sny56posuffadh5a367n5vi4uq 281 6 the the DT work_sny56posuffadh5a367n5vi4uq 281 7 sites site NNS work_sny56posuffadh5a367n5vi4uq 281 8 of of IN work_sny56posuffadh5a367n5vi4uq 281 9 excision excision NN work_sny56posuffadh5a367n5vi4uq 281 10 both both CC work_sny56posuffadh5a367n5vi4uq 281 11 inside inside IN work_sny56posuffadh5a367n5vi4uq 281 12 and and CC work_sny56posuffadh5a367n5vi4uq 281 13 outside outside IN work_sny56posuffadh5a367n5vi4uq 281 14 the the DT work_sny56posuffadh5a367n5vi4uq 281 15 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 281 16 suggests suggest VBZ work_sny56posuffadh5a367n5vi4uq 281 17 that that IN work_sny56posuffadh5a367n5vi4uq 281 18 the the DT work_sny56posuffadh5a367n5vi4uq 281 19 requirement requirement NN work_sny56posuffadh5a367n5vi4uq 281 20 of of IN work_sny56posuffadh5a367n5vi4uq 281 21 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 281 22 - - HYPH work_sny56posuffadh5a367n5vi4uq 281 23 donor donor NN work_sny56posuffadh5a367n5vi4uq 281 24 junction junction NN work_sny56posuffadh5a367n5vi4uq 281 25 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 281 26 for for IN work_sny56posuffadh5a367n5vi4uq 281 27 excision excision NN work_sny56posuffadh5a367n5vi4uq 281 28 is be VBZ work_sny56posuffadh5a367n5vi4uq 281 29 not not RB work_sny56posuffadh5a367n5vi4uq 281 30 base base NN work_sny56posuffadh5a367n5vi4uq 281 31 - - HYPH work_sny56posuffadh5a367n5vi4uq 281 32 pair pair NN work_sny56posuffadh5a367n5vi4uq 281 33 specific specific NN work_sny56posuffadh5a367n5vi4uq 281 34 . . . work_sny56posuffadh5a367n5vi4uq 282 1 Replacement replacement NN work_sny56posuffadh5a367n5vi4uq 282 2 of of IN work_sny56posuffadh5a367n5vi4uq 282 3 the the DT work_sny56posuffadh5a367n5vi4uq 282 4 TA- TA- NNP work_sny56posuffadh5a367n5vi4uq 282 5 dinucleotide dinucleotide NN work_sny56posuffadh5a367n5vi4uq 282 6 , , , work_sny56posuffadh5a367n5vi4uq 282 7 or or CC work_sny56posuffadh5a367n5vi4uq 282 8 of of IN work_sny56posuffadh5a367n5vi4uq 282 9 single single JJ work_sny56posuffadh5a367n5vi4uq 282 10 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 282 11 at at IN work_sny56posuffadh5a367n5vi4uq 282 12 the the DT work_sny56posuffadh5a367n5vi4uq 282 13 end end NN work_sny56posuffadh5a367n5vi4uq 282 14 of of IN work_sny56posuffadh5a367n5vi4uq 282 15 the the DT work_sny56posuffadh5a367n5vi4uq 282 16 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 282 17 , , , work_sny56posuffadh5a367n5vi4uq 282 18 reduced reduce VBN work_sny56posuffadh5a367n5vi4uq 282 19 but but CC work_sny56posuffadh5a367n5vi4uq 282 20 did do VBD work_sny56posuffadh5a367n5vi4uq 282 21 not not RB work_sny56posuffadh5a367n5vi4uq 282 22 abolish abolish VB work_sny56posuffadh5a367n5vi4uq 282 23 excision excision NN work_sny56posuffadh5a367n5vi4uq 282 24 activity activity NN work_sny56posuffadh5a367n5vi4uq 282 25 . . . work_sny56posuffadh5a367n5vi4uq 283 1 These these DT work_sny56posuffadh5a367n5vi4uq 283 2 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 283 3 may may MD work_sny56posuffadh5a367n5vi4uq 283 4 work work VB work_sny56posuffadh5a367n5vi4uq 283 5 synergistically synergistically RB work_sny56posuffadh5a367n5vi4uq 283 6 . . . work_sny56posuffadh5a367n5vi4uq 284 1 Whereas whereas IN work_sny56posuffadh5a367n5vi4uq 284 2 point point NN work_sny56posuffadh5a367n5vi4uq 284 3 mutation mutation NN work_sny56posuffadh5a367n5vi4uq 284 4 at at IN work_sny56posuffadh5a367n5vi4uq 284 5 either either DT work_sny56posuffadh5a367n5vi4uq 284 6 position position NN work_sny56posuffadh5a367n5vi4uq 284 7 1 1 CD work_sny56posuffadh5a367n5vi4uq 284 8 or or CC work_sny56posuffadh5a367n5vi4uq 284 9 3 3 CD work_sny56posuffadh5a367n5vi4uq 284 10 at at IN work_sny56posuffadh5a367n5vi4uq 284 11 the the DT work_sny56posuffadh5a367n5vi4uq 284 12 end end NN work_sny56posuffadh5a367n5vi4uq 284 13 of of IN work_sny56posuffadh5a367n5vi4uq 284 14 the the DT work_sny56posuffadh5a367n5vi4uq 284 15 left left JJ work_sny56posuffadh5a367n5vi4uq 284 16 IR IR NNP work_sny56posuffadh5a367n5vi4uq 284 17 reduced reduce VBN work_sny56posuffadh5a367n5vi4uq 284 18 relative relative JJ work_sny56posuffadh5a367n5vi4uq 284 19 excision excision NN work_sny56posuffadh5a367n5vi4uq 284 20 activity activity NN work_sny56posuffadh5a367n5vi4uq 284 21 to to IN work_sny56posuffadh5a367n5vi4uq 284 22 11 11 CD work_sny56posuffadh5a367n5vi4uq 284 23 and and CC work_sny56posuffadh5a367n5vi4uq 284 24 18 18 CD work_sny56posuffadh5a367n5vi4uq 284 25 % % NN work_sny56posuffadh5a367n5vi4uq 284 26 , , , work_sny56posuffadh5a367n5vi4uq 284 27 respectively respectively RB work_sny56posuffadh5a367n5vi4uq 284 28 , , , work_sny56posuffadh5a367n5vi4uq 284 29 the the DT work_sny56posuffadh5a367n5vi4uq 284 30 double double JJ work_sny56posuffadh5a367n5vi4uq 284 31 mutation mutation NN work_sny56posuffadh5a367n5vi4uq 284 32 reduced reduce VBD work_sny56posuffadh5a367n5vi4uq 284 33 the the DT work_sny56posuffadh5a367n5vi4uq 284 34 relative relative JJ work_sny56posuffadh5a367n5vi4uq 284 35 excision excision NN work_sny56posuffadh5a367n5vi4uq 284 36 activity activity NN work_sny56posuffadh5a367n5vi4uq 284 37 below below IN work_sny56posuffadh5a367n5vi4uq 284 38 the the DT work_sny56posuffadh5a367n5vi4uq 284 39 detectable detectable JJ work_sny56posuffadh5a367n5vi4uq 284 40 limit limit NN work_sny56posuffadh5a367n5vi4uq 284 41 , , , work_sny56posuffadh5a367n5vi4uq 284 42 about about RB work_sny56posuffadh5a367n5vi4uq 284 43 5 5 CD work_sny56posuffadh5a367n5vi4uq 284 44 % % NN work_sny56posuffadh5a367n5vi4uq 284 45 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 284 46 11 11 CD work_sny56posuffadh5a367n5vi4uq 284 47 % % NN work_sny56posuffadh5a367n5vi4uq 284 48 × × CD work_sny56posuffadh5a367n5vi4uq 284 49 18 18 CD work_sny56posuffadh5a367n5vi4uq 284 50 % % NN work_sny56posuffadh5a367n5vi4uq 284 51 < < XX work_sny56posuffadh5a367n5vi4uq 284 52 5 5 CD work_sny56posuffadh5a367n5vi4uq 284 53 % % NN work_sny56posuffadh5a367n5vi4uq 284 54 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 284 55 . . . work_sny56posuffadh5a367n5vi4uq 285 1 These these DT work_sny56posuffadh5a367n5vi4uq 285 2 results result NNS work_sny56posuffadh5a367n5vi4uq 285 3 suggest suggest VBP work_sny56posuffadh5a367n5vi4uq 285 4 that that IN work_sny56posuffadh5a367n5vi4uq 285 5 the the DT work_sny56posuffadh5a367n5vi4uq 285 6 nucleotides nucleotide NNS work_sny56posuffadh5a367n5vi4uq 285 7 in in IN work_sny56posuffadh5a367n5vi4uq 285 8 this this DT work_sny56posuffadh5a367n5vi4uq 285 9 region region NN work_sny56posuffadh5a367n5vi4uq 285 10 may may MD work_sny56posuffadh5a367n5vi4uq 285 11 contribute contribute VB work_sny56posuffadh5a367n5vi4uq 285 12 to to IN work_sny56posuffadh5a367n5vi4uq 285 13 a a DT work_sny56posuffadh5a367n5vi4uq 285 14 certain certain JJ work_sny56posuffadh5a367n5vi4uq 285 15 environment environment NN work_sny56posuffadh5a367n5vi4uq 285 16 to to TO work_sny56posuffadh5a367n5vi4uq 285 17 affect affect VB work_sny56posuffadh5a367n5vi4uq 285 18 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 285 19 – – : work_sny56posuffadh5a367n5vi4uq 285 20 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 285 21 interactions interaction NNS work_sny56posuffadh5a367n5vi4uq 285 22 . . . work_sny56posuffadh5a367n5vi4uq 286 1 This this DT work_sny56posuffadh5a367n5vi4uq 286 2 environment environment NN work_sny56posuffadh5a367n5vi4uq 286 3 could could MD work_sny56posuffadh5a367n5vi4uq 286 4 have have VB work_sny56posuffadh5a367n5vi4uq 286 5 certain certain JJ work_sny56posuffadh5a367n5vi4uq 286 6 physical physical JJ work_sny56posuffadh5a367n5vi4uq 286 7 properties property NNS work_sny56posuffadh5a367n5vi4uq 286 8 required require VBN work_sny56posuffadh5a367n5vi4uq 286 9 for for IN work_sny56posuffadh5a367n5vi4uq 286 10 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 286 11 catalytic catalytic JJ work_sny56posuffadh5a367n5vi4uq 286 12 activity activity NN work_sny56posuffadh5a367n5vi4uq 286 13 , , , work_sny56posuffadh5a367n5vi4uq 286 14 similar similar JJ work_sny56posuffadh5a367n5vi4uq 286 15 to to IN work_sny56posuffadh5a367n5vi4uq 286 16 the the DT work_sny56posuffadh5a367n5vi4uq 286 17 one one NN work_sny56posuffadh5a367n5vi4uq 286 18 observed observe VBN work_sny56posuffadh5a367n5vi4uq 286 19 at at IN work_sny56posuffadh5a367n5vi4uq 286 20 the the DT work_sny56posuffadh5a367n5vi4uq 286 21 target target NN work_sny56posuffadh5a367n5vi4uq 286 22 site site NN work_sny56posuffadh5a367n5vi4uq 286 23 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 286 24 15,17 15,17 CD work_sny56posuffadh5a367n5vi4uq 286 25 ; ; : work_sny56posuffadh5a367n5vi4uq 286 26 G. G. NNP work_sny56posuffadh5a367n5vi4uq 286 27 Liu Liu NNP work_sny56posuffadh5a367n5vi4uq 286 28 , , , work_sny56posuffadh5a367n5vi4uq 286 29 in in IN work_sny56posuffadh5a367n5vi4uq 286 30 preparation preparation NN work_sny56posuffadh5a367n5vi4uq 286 31 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 286 32 . . . work_sny56posuffadh5a367n5vi4uq 287 1 Perturbing perturb VBG work_sny56posuffadh5a367n5vi4uq 287 2 these these DT work_sny56posuffadh5a367n5vi4uq 287 3 interactions interaction NNS work_sny56posuffadh5a367n5vi4uq 287 4 would would MD work_sny56posuffadh5a367n5vi4uq 287 5 result result VB work_sny56posuffadh5a367n5vi4uq 287 6 in in IN work_sny56posuffadh5a367n5vi4uq 287 7 a a DT work_sny56posuffadh5a367n5vi4uq 287 8 less less RBR work_sny56posuffadh5a367n5vi4uq 287 9 favorable favorable JJ work_sny56posuffadh5a367n5vi4uq 287 10 environment environment NN work_sny56posuffadh5a367n5vi4uq 287 11 that that WDT work_sny56posuffadh5a367n5vi4uq 287 12 would would MD work_sny56posuffadh5a367n5vi4uq 287 13 lead lead VB work_sny56posuffadh5a367n5vi4uq 287 14 to to IN work_sny56posuffadh5a367n5vi4uq 287 15 a a DT work_sny56posuffadh5a367n5vi4uq 287 16 decrease decrease NN work_sny56posuffadh5a367n5vi4uq 287 17 in in IN work_sny56posuffadh5a367n5vi4uq 287 18 the the DT work_sny56posuffadh5a367n5vi4uq 287 19 rate rate NN work_sny56posuffadh5a367n5vi4uq 287 20 of of IN work_sny56posuffadh5a367n5vi4uq 287 21 excision excision NN work_sny56posuffadh5a367n5vi4uq 287 22 and and CC work_sny56posuffadh5a367n5vi4uq 287 23 an an DT work_sny56posuffadh5a367n5vi4uq 287 24 approximately approximately RB work_sny56posuffadh5a367n5vi4uq 287 25 equal equal JJ work_sny56posuffadh5a367n5vi4uq 287 26 decrease decrease NN work_sny56posuffadh5a367n5vi4uq 287 27 in in IN work_sny56posuffadh5a367n5vi4uq 287 28 the the DT work_sny56posuffadh5a367n5vi4uq 287 29 rate rate NN work_sny56posuffadh5a367n5vi4uq 287 30 of of IN work_sny56posuffadh5a367n5vi4uq 287 31 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 287 32 . . . work_sny56posuffadh5a367n5vi4uq 288 1 If if IN work_sny56posuffadh5a367n5vi4uq 288 2 this this DT work_sny56posuffadh5a367n5vi4uq 288 3 hypothesis hypothesis NN work_sny56posuffadh5a367n5vi4uq 288 4 is be VBZ work_sny56posuffadh5a367n5vi4uq 288 5 true true JJ work_sny56posuffadh5a367n5vi4uq 288 6 , , , work_sny56posuffadh5a367n5vi4uq 288 7 then then RB work_sny56posuffadh5a367n5vi4uq 288 8 the the DT work_sny56posuffadh5a367n5vi4uq 288 9 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 288 10 sequence sequence NN work_sny56posuffadh5a367n5vi4uq 288 11 of of IN work_sny56posuffadh5a367n5vi4uq 288 12 the the DT work_sny56posuffadh5a367n5vi4uq 288 13 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 288 14 outside outside IN work_sny56posuffadh5a367n5vi4uq 288 15 the the DT work_sny56posuffadh5a367n5vi4uq 288 16 TA TA NNP work_sny56posuffadh5a367n5vi4uq 288 17 - - HYPH work_sny56posuffadh5a367n5vi4uq 288 18 dinucleotides dinucleotide NNS work_sny56posuffadh5a367n5vi4uq 288 19 might may MD work_sny56posuffadh5a367n5vi4uq 288 20 influence influence VB work_sny56posuffadh5a367n5vi4uq 288 21 transposon transposon JJ work_sny56posuffadh5a367n5vi4uq 288 22 activity activity NN work_sny56posuffadh5a367n5vi4uq 288 23 as as RB work_sny56posuffadh5a367n5vi4uq 288 24 well well RB work_sny56posuffadh5a367n5vi4uq 288 25 . . . work_sny56posuffadh5a367n5vi4uq 289 1 Thus thus RB work_sny56posuffadh5a367n5vi4uq 289 2 , , , work_sny56posuffadh5a367n5vi4uq 289 3 with with IN work_sny56posuffadh5a367n5vi4uq 289 4 the the DT work_sny56posuffadh5a367n5vi4uq 289 5 excision excision NN work_sny56posuffadh5a367n5vi4uq 289 6 assay assay NN work_sny56posuffadh5a367n5vi4uq 289 7 , , , work_sny56posuffadh5a367n5vi4uq 289 8 we -PRON- PRP work_sny56posuffadh5a367n5vi4uq 289 9 should should MD work_sny56posuffadh5a367n5vi4uq 289 10 be be VB work_sny56posuffadh5a367n5vi4uq 289 11 able able JJ work_sny56posuffadh5a367n5vi4uq 289 12 to to TO work_sny56posuffadh5a367n5vi4uq 289 13 improve improve VB work_sny56posuffadh5a367n5vi4uq 289 14 further further RB work_sny56posuffadh5a367n5vi4uq 289 15 the the DT work_sny56posuffadh5a367n5vi4uq 289 16 flanking flank VBG work_sny56posuffadh5a367n5vi4uq 289 17 sites site NNS work_sny56posuffadh5a367n5vi4uq 289 18 , , , work_sny56posuffadh5a367n5vi4uq 289 19 which which WDT work_sny56posuffadh5a367n5vi4uq 289 20 , , , work_sny56posuffadh5a367n5vi4uq 289 21 like like IN work_sny56posuffadh5a367n5vi4uq 289 22 improving improve VBG work_sny56posuffadh5a367n5vi4uq 289 23 the the DT work_sny56posuffadh5a367n5vi4uq 289 24 activity activity NN work_sny56posuffadh5a367n5vi4uq 289 25 of of IN work_sny56posuffadh5a367n5vi4uq 289 26 SB SB NNP work_sny56posuffadh5a367n5vi4uq 289 27 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 289 28 [ [ -LRB- work_sny56posuffadh5a367n5vi4uq 289 29 13 13 CD work_sny56posuffadh5a367n5vi4uq 289 30 ] ] -RRB- work_sny56posuffadh5a367n5vi4uq 289 31 , , , work_sny56posuffadh5a367n5vi4uq 289 32 should should MD work_sny56posuffadh5a367n5vi4uq 289 33 lead lead VB work_sny56posuffadh5a367n5vi4uq 289 34 to to IN work_sny56posuffadh5a367n5vi4uq 289 35 more more RBR work_sny56posuffadh5a367n5vi4uq 289 36 a a DT work_sny56posuffadh5a367n5vi4uq 289 37 more more RBR work_sny56posuffadh5a367n5vi4uq 289 38 powerful powerful JJ work_sny56posuffadh5a367n5vi4uq 289 39 transposon transposon JJ work_sny56posuffadh5a367n5vi4uq 289 40 system system NN work_sny56posuffadh5a367n5vi4uq 289 41 . . . work_sny56posuffadh5a367n5vi4uq 290 1 Our -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 290 2 results result NNS work_sny56posuffadh5a367n5vi4uq 290 3 have have VBP work_sny56posuffadh5a367n5vi4uq 290 4 considerable considerable JJ work_sny56posuffadh5a367n5vi4uq 290 5 potential potential NN work_sny56posuffadh5a367n5vi4uq 290 6 for for IN work_sny56posuffadh5a367n5vi4uq 290 7 developing develop VBG work_sny56posuffadh5a367n5vi4uq 290 8 non non JJ work_sny56posuffadh5a367n5vi4uq 290 9 - - JJ work_sny56posuffadh5a367n5vi4uq 290 10 viral viral JJ work_sny56posuffadh5a367n5vi4uq 290 11 , , , work_sny56posuffadh5a367n5vi4uq 290 12 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 290 13 - - HYPH work_sny56posuffadh5a367n5vi4uq 290 14 mediated mediate VBN work_sny56posuffadh5a367n5vi4uq 290 15 , , , work_sny56posuffadh5a367n5vi4uq 290 16 gene gene NN work_sny56posuffadh5a367n5vi4uq 290 17 - - HYPH work_sny56posuffadh5a367n5vi4uq 290 18 transfer transfer NN work_sny56posuffadh5a367n5vi4uq 290 19 vectors vector NNS work_sny56posuffadh5a367n5vi4uq 290 20 for for IN work_sny56posuffadh5a367n5vi4uq 290 21 human human JJ work_sny56posuffadh5a367n5vi4uq 290 22 therapy therapy NN work_sny56posuffadh5a367n5vi4uq 290 23 . . . work_sny56posuffadh5a367n5vi4uq 291 1 At at IN work_sny56posuffadh5a367n5vi4uq 291 2 present present NN work_sny56posuffadh5a367n5vi4uq 291 3 , , , work_sny56posuffadh5a367n5vi4uq 291 4 the the DT work_sny56posuffadh5a367n5vi4uq 291 5 only only JJ work_sny56posuffadh5a367n5vi4uq 291 6 method method NN work_sny56posuffadh5a367n5vi4uq 291 7 for for IN work_sny56posuffadh5a367n5vi4uq 291 8 evaluation evaluation NN work_sny56posuffadh5a367n5vi4uq 291 9 of of IN work_sny56posuffadh5a367n5vi4uq 291 10 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 291 11 events event NNS work_sny56posuffadh5a367n5vi4uq 291 12 in in IN work_sny56posuffadh5a367n5vi4uq 291 13 organs organ NNS work_sny56posuffadh5a367n5vi4uq 291 14 of of IN work_sny56posuffadh5a367n5vi4uq 291 15 multi multi JJ work_sny56posuffadh5a367n5vi4uq 291 16 - - JJ work_sny56posuffadh5a367n5vi4uq 291 17 cellular cellular JJ work_sny56posuffadh5a367n5vi4uq 291 18 animals animal NNS work_sny56posuffadh5a367n5vi4uq 291 19 is be VBZ work_sny56posuffadh5a367n5vi4uq 291 20 to to TO work_sny56posuffadh5a367n5vi4uq 291 21 sequence sequence VB work_sny56posuffadh5a367n5vi4uq 291 22 insertion insertion NN work_sny56posuffadh5a367n5vi4uq 291 23 sites site NNS work_sny56posuffadh5a367n5vi4uq 291 24 in in IN work_sny56posuffadh5a367n5vi4uq 291 25 chromosomes chromosome NNS work_sny56posuffadh5a367n5vi4uq 291 26 of of IN work_sny56posuffadh5a367n5vi4uq 291 27 treated treat VBN work_sny56posuffadh5a367n5vi4uq 291 28 animals animal NNS work_sny56posuffadh5a367n5vi4uq 291 29 because because IN work_sny56posuffadh5a367n5vi4uq 291 30 each each DT work_sny56posuffadh5a367n5vi4uq 291 31 cell cell NN work_sny56posuffadh5a367n5vi4uq 291 32 in in IN work_sny56posuffadh5a367n5vi4uq 291 33 which which WDT work_sny56posuffadh5a367n5vi4uq 291 34 the the DT work_sny56posuffadh5a367n5vi4uq 291 35 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 291 36 integrates integrates NNPS work_sny56posuffadh5a367n5vi4uq 291 37 is be VBZ work_sny56posuffadh5a367n5vi4uq 291 38 a a DT work_sny56posuffadh5a367n5vi4uq 291 39 separate separate JJ work_sny56posuffadh5a367n5vi4uq 291 40 event event NN work_sny56posuffadh5a367n5vi4uq 291 41 that that WDT work_sny56posuffadh5a367n5vi4uq 291 42 is be VBZ work_sny56posuffadh5a367n5vi4uq 291 43 non non JJ work_sny56posuffadh5a367n5vi4uq 291 44 - - JJ work_sny56posuffadh5a367n5vi4uq 291 45 clonal clonal JJ work_sny56posuffadh5a367n5vi4uq 291 46 . . . work_sny56posuffadh5a367n5vi4uq 292 1 This this DT work_sny56posuffadh5a367n5vi4uq 292 2 procedure procedure NN work_sny56posuffadh5a367n5vi4uq 292 3 is be VBZ work_sny56posuffadh5a367n5vi4uq 292 4 exceptionally exceptionally RB work_sny56posuffadh5a367n5vi4uq 292 5 labor- labor- FW work_sny56posuffadh5a367n5vi4uq 292 6 intensive intensive JJ work_sny56posuffadh5a367n5vi4uq 292 7 and and CC work_sny56posuffadh5a367n5vi4uq 292 8 non non JJ work_sny56posuffadh5a367n5vi4uq 292 9 - - JJ work_sny56posuffadh5a367n5vi4uq 292 10 quantitative quantitative JJ work_sny56posuffadh5a367n5vi4uq 292 11 . . . work_sny56posuffadh5a367n5vi4uq 293 1 In in IN work_sny56posuffadh5a367n5vi4uq 293 2 contrast contrast NN work_sny56posuffadh5a367n5vi4uq 293 3 , , , work_sny56posuffadh5a367n5vi4uq 293 4 by by IN work_sny56posuffadh5a367n5vi4uq 293 5 employing employ VBG work_sny56posuffadh5a367n5vi4uq 293 6 the the DT work_sny56posuffadh5a367n5vi4uq 293 7 excision excision NN work_sny56posuffadh5a367n5vi4uq 293 8 assay assay VB work_sny56posuffadh5a367n5vi4uq 293 9 a a DT work_sny56posuffadh5a367n5vi4uq 293 10 few few JJ work_sny56posuffadh5a367n5vi4uq 293 11 days day NNS work_sny56posuffadh5a367n5vi4uq 293 12 after after IN work_sny56posuffadh5a367n5vi4uq 293 13 gene gene NN work_sny56posuffadh5a367n5vi4uq 293 14 transfer transfer NN work_sny56posuffadh5a367n5vi4uq 293 15 , , , work_sny56posuffadh5a367n5vi4uq 293 16 investigators investigator NNS work_sny56posuffadh5a367n5vi4uq 293 17 will will MD work_sny56posuffadh5a367n5vi4uq 293 18 be be VB work_sny56posuffadh5a367n5vi4uq 293 19 able able JJ work_sny56posuffadh5a367n5vi4uq 293 20 to to TO work_sny56posuffadh5a367n5vi4uq 293 21 approximate approximate VB work_sny56posuffadh5a367n5vi4uq 293 22 efficiently efficiently RB work_sny56posuffadh5a367n5vi4uq 293 23 and and CC work_sny56posuffadh5a367n5vi4uq 293 24 quickly quickly RB work_sny56posuffadh5a367n5vi4uq 293 25 the the DT work_sny56posuffadh5a367n5vi4uq 293 26 levels level NNS work_sny56posuffadh5a367n5vi4uq 293 27 of of IN work_sny56posuffadh5a367n5vi4uq 293 28 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 293 29 into into IN work_sny56posuffadh5a367n5vi4uq 293 30 chromosomes chromosome NNS work_sny56posuffadh5a367n5vi4uq 293 31 of of IN work_sny56posuffadh5a367n5vi4uq 293 32 a a DT work_sny56posuffadh5a367n5vi4uq 293 33 multi multi JJ work_sny56posuffadh5a367n5vi4uq 293 34 - - JJ work_sny56posuffadh5a367n5vi4uq 293 35 cellular cellular JJ work_sny56posuffadh5a367n5vi4uq 293 36 organ organ NN work_sny56posuffadh5a367n5vi4uq 293 37 . . . work_sny56posuffadh5a367n5vi4uq 294 1 Thus thus RB work_sny56posuffadh5a367n5vi4uq 294 2 , , , work_sny56posuffadh5a367n5vi4uq 294 3 the the DT work_sny56posuffadh5a367n5vi4uq 294 4 excision excision NN work_sny56posuffadh5a367n5vi4uq 294 5 assay assay NN work_sny56posuffadh5a367n5vi4uq 294 6 should should MD work_sny56posuffadh5a367n5vi4uq 294 7 be be VB work_sny56posuffadh5a367n5vi4uq 294 8 extremely extremely RB work_sny56posuffadh5a367n5vi4uq 294 9 useful useful JJ work_sny56posuffadh5a367n5vi4uq 294 10 for for IN work_sny56posuffadh5a367n5vi4uq 294 11 evaluation evaluation NN work_sny56posuffadh5a367n5vi4uq 294 12 of of IN work_sny56posuffadh5a367n5vi4uq 294 13 parameters parameter NNS work_sny56posuffadh5a367n5vi4uq 294 14 affecting affect VBG work_sny56posuffadh5a367n5vi4uq 294 15 gene gene NN work_sny56posuffadh5a367n5vi4uq 294 16 delivery delivery NN work_sny56posuffadh5a367n5vi4uq 294 17 using use VBG work_sny56posuffadh5a367n5vi4uq 294 18 plasmids plasmid NNS work_sny56posuffadh5a367n5vi4uq 294 19 as as IN work_sny56posuffadh5a367n5vi4uq 294 20 donor donor NN work_sny56posuffadh5a367n5vi4uq 294 21 vehicles vehicle NNS work_sny56posuffadh5a367n5vi4uq 294 22 . . . work_sny56posuffadh5a367n5vi4uq 295 1 Acknowledgements acknowledgement NNS work_sny56posuffadh5a367n5vi4uq 295 2 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 295 3 thank thank VBP work_sny56posuffadh5a367n5vi4uq 295 4 Dr. Dr. NNP work_sny56posuffadh5a367n5vi4uq 295 5 Akihiko Akihiko NNP work_sny56posuffadh5a367n5vi4uq 295 6 Koga Koga NNP work_sny56posuffadh5a367n5vi4uq 295 7 and and CC work_sny56posuffadh5a367n5vi4uq 295 8 Dr. Dr. NNP work_sny56posuffadh5a367n5vi4uq 295 9 Koichi Koichi NNP work_sny56posuffadh5a367n5vi4uq 295 10 Kawakami Kawakami NNP work_sny56posuffadh5a367n5vi4uq 295 11 for for IN work_sny56posuffadh5a367n5vi4uq 295 12 assistance assistance NN work_sny56posuffadh5a367n5vi4uq 295 13 in in IN work_sny56posuffadh5a367n5vi4uq 295 14 development development NN work_sny56posuffadh5a367n5vi4uq 295 15 of of IN work_sny56posuffadh5a367n5vi4uq 295 16 the the DT work_sny56posuffadh5a367n5vi4uq 295 17 excision excision NN work_sny56posuffadh5a367n5vi4uq 295 18 assay assay NN work_sny56posuffadh5a367n5vi4uq 295 19 . . . work_sny56posuffadh5a367n5vi4uq 296 1 We -PRON- PRP work_sny56posuffadh5a367n5vi4uq 296 2 are be VBP work_sny56posuffadh5a367n5vi4uq 296 3 especially especially RB work_sny56posuffadh5a367n5vi4uq 296 4 grateful grateful JJ work_sny56posuffadh5a367n5vi4uq 296 5 to to IN work_sny56posuffadh5a367n5vi4uq 296 6 Dr. Dr. NNP work_sny56posuffadh5a367n5vi4uq 296 7 Yusuke Yusuke NNP work_sny56posuffadh5a367n5vi4uq 296 8 Kamachi Kamachi NNP work_sny56posuffadh5a367n5vi4uq 296 9 and and CC work_sny56posuffadh5a367n5vi4uq 296 10 Heather Heather NNP work_sny56posuffadh5a367n5vi4uq 296 11 Gardner Gardner NNP work_sny56posuffadh5a367n5vi4uq 296 12 for for IN work_sny56posuffadh5a367n5vi4uq 296 13 instructive instructive JJ work_sny56posuffadh5a367n5vi4uq 296 14 discussions discussion NNS work_sny56posuffadh5a367n5vi4uq 296 15 . . . work_sny56posuffadh5a367n5vi4uq 297 1 Many many JJ work_sny56posuffadh5a367n5vi4uq 297 2 thanks thank NNS work_sny56posuffadh5a367n5vi4uq 297 3 to to IN work_sny56posuffadh5a367n5vi4uq 297 4 Stephen Stephen NNP work_sny56posuffadh5a367n5vi4uq 297 5 Ekker Ekker NNP work_sny56posuffadh5a367n5vi4uq 297 6 , , , work_sny56posuffadh5a367n5vi4uq 297 7 Scott Scott NNP work_sny56posuffadh5a367n5vi4uq 297 8 McIvor McIvor NNP work_sny56posuffadh5a367n5vi4uq 297 9 , , , work_sny56posuffadh5a367n5vi4uq 297 10 David David NNP work_sny56posuffadh5a367n5vi4uq 297 11 Largaespada Largaespada NNP work_sny56posuffadh5a367n5vi4uq 297 12 and and CC work_sny56posuffadh5a367n5vi4uq 297 13 other other JJ work_sny56posuffadh5a367n5vi4uq 297 14 members member NNS work_sny56posuffadh5a367n5vi4uq 297 15 of of IN work_sny56posuffadh5a367n5vi4uq 297 16 the the DT work_sny56posuffadh5a367n5vi4uq 297 17 Beckman Beckman NNP work_sny56posuffadh5a367n5vi4uq 297 18 Center Center NNP work_sny56posuffadh5a367n5vi4uq 297 19 for for IN work_sny56posuffadh5a367n5vi4uq 297 20 Transposon Transposon NNP work_sny56posuffadh5a367n5vi4uq 297 21 Research Research NNP work_sny56posuffadh5a367n5vi4uq 297 22 for for IN work_sny56posuffadh5a367n5vi4uq 297 23 insights insight NNS work_sny56posuffadh5a367n5vi4uq 297 24 and and CC work_sny56posuffadh5a367n5vi4uq 297 25 guidance guidance NN work_sny56posuffadh5a367n5vi4uq 297 26 and and CC work_sny56posuffadh5a367n5vi4uq 297 27 David David NNP work_sny56posuffadh5a367n5vi4uq 297 28 Erickson Erickson NNP work_sny56posuffadh5a367n5vi4uq 297 29 for for IN work_sny56posuffadh5a367n5vi4uq 297 30 help help NN work_sny56posuffadh5a367n5vi4uq 297 31 with with IN work_sny56posuffadh5a367n5vi4uq 297 32 the the DT work_sny56posuffadh5a367n5vi4uq 297 33 manuscript manuscript NN work_sny56posuffadh5a367n5vi4uq 297 34 and and CC work_sny56posuffadh5a367n5vi4uq 297 35 tables table NNS work_sny56posuffadh5a367n5vi4uq 297 36 . . . work_sny56posuffadh5a367n5vi4uq 298 1 This this DT work_sny56posuffadh5a367n5vi4uq 298 2 work work NN work_sny56posuffadh5a367n5vi4uq 298 3 was be VBD work_sny56posuffadh5a367n5vi4uq 298 4 supported support VBN work_sny56posuffadh5a367n5vi4uq 298 5 by by IN work_sny56posuffadh5a367n5vi4uq 298 6 grants grant NNS work_sny56posuffadh5a367n5vi4uq 298 7 from from IN work_sny56posuffadh5a367n5vi4uq 298 8 the the DT work_sny56posuffadh5a367n5vi4uq 298 9 Arnold Arnold NNP work_sny56posuffadh5a367n5vi4uq 298 10 and and CC work_sny56posuffadh5a367n5vi4uq 298 11 Mabel Mabel NNP work_sny56posuffadh5a367n5vi4uq 298 12 Beckman Beckman NNP work_sny56posuffadh5a367n5vi4uq 298 13 Foundation Foundation NNP work_sny56posuffadh5a367n5vi4uq 298 14 and and CC work_sny56posuffadh5a367n5vi4uq 298 15 the the DT work_sny56posuffadh5a367n5vi4uq 298 16 NIH NIH NNP work_sny56posuffadh5a367n5vi4uq 298 17 ( ( -LRB- work_sny56posuffadh5a367n5vi4uq 298 18 NCRR NCRR NNP work_sny56posuffadh5a367n5vi4uq 298 19 R01 R01 NNP work_sny56posuffadh5a367n5vi4uq 298 20 - - HYPH work_sny56posuffadh5a367n5vi4uq 298 21 066525 066525 CD work_sny56posuffadh5a367n5vi4uq 298 22 - - HYPH work_sny56posuffadh5a367n5vi4uq 298 23 07 07 CD work_sny56posuffadh5a367n5vi4uq 298 24 and and CC work_sny56posuffadh5a367n5vi4uq 298 25 NICHD NICHD NNP work_sny56posuffadh5a367n5vi4uq 298 26 P01-HD32652 P01-HD32652 NNP work_sny56posuffadh5a367n5vi4uq 298 27 ) ) -RRB- work_sny56posuffadh5a367n5vi4uq 298 28 . . . work_sny56posuffadh5a367n5vi4uq 299 1 ELA ELA NNP work_sny56posuffadh5a367n5vi4uq 299 2 was be VBD work_sny56posuffadh5a367n5vi4uq 299 3 supported support VBN work_sny56posuffadh5a367n5vi4uq 299 4 by by IN work_sny56posuffadh5a367n5vi4uq 299 5 a a DT work_sny56posuffadh5a367n5vi4uq 299 6 Viking Viking NNP work_sny56posuffadh5a367n5vi4uq 299 7 Children Children NNP work_sny56posuffadh5a367n5vi4uq 299 8 ’s ’s POS work_sny56posuffadh5a367n5vi4uq 299 9 Fund Fund NNP work_sny56posuffadh5a367n5vi4uq 299 10 Grant Grant NNP work_sny56posuffadh5a367n5vi4uq 299 11 - - HYPH work_sny56posuffadh5a367n5vi4uq 299 12 in in IN work_sny56posuffadh5a367n5vi4uq 299 13 - - HYPH work_sny56posuffadh5a367n5vi4uq 299 14 Aid aid NN work_sny56posuffadh5a367n5vi4uq 299 15 . . . work_sny56posuffadh5a367n5vi4uq 300 1 Copyright copyright NN work_sny56posuffadh5a367n5vi4uq 300 2   CD work_sny56posuffadh5a367n5vi4uq 300 3 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 300 4 John John NNP work_sny56posuffadh5a367n5vi4uq 300 5 Wiley Wiley NNP work_sny56posuffadh5a367n5vi4uq 300 6 & & CC work_sny56posuffadh5a367n5vi4uq 300 7 Sons Sons NNPS work_sny56posuffadh5a367n5vi4uq 300 8 , , , work_sny56posuffadh5a367n5vi4uq 300 9 Ltd. Ltd. NNP work_sny56posuffadh5a367n5vi4uq 300 10 J J NNP work_sny56posuffadh5a367n5vi4uq 300 11 Gene Gene NNP work_sny56posuffadh5a367n5vi4uq 300 12 Med Med NNP work_sny56posuffadh5a367n5vi4uq 300 13 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 300 14 ; ; : work_sny56posuffadh5a367n5vi4uq 300 15 6 6 CD work_sny56posuffadh5a367n5vi4uq 300 16 : : : work_sny56posuffadh5a367n5vi4uq 300 17 574 574 CD work_sny56posuffadh5a367n5vi4uq 300 18 – – : work_sny56posuffadh5a367n5vi4uq 300 19 583 583 CD work_sny56posuffadh5a367n5vi4uq 300 20 . . . work_sny56posuffadh5a367n5vi4uq 301 1 Excision excision NN work_sny56posuffadh5a367n5vi4uq 301 2 of of IN work_sny56posuffadh5a367n5vi4uq 301 3 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 301 4 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 301 5 Transposons Transposons NNP work_sny56posuffadh5a367n5vi4uq 301 6 583 583 CD work_sny56posuffadh5a367n5vi4uq 301 7 References reference NNS work_sny56posuffadh5a367n5vi4uq 301 8 1 1 CD work_sny56posuffadh5a367n5vi4uq 301 9 . . . work_sny56posuffadh5a367n5vi4uq 302 1 Plasterk Plasterk NNP work_sny56posuffadh5a367n5vi4uq 302 2 RH RH NNP work_sny56posuffadh5a367n5vi4uq 302 3 . . . work_sny56posuffadh5a367n5vi4uq 303 1 The the DT work_sny56posuffadh5a367n5vi4uq 303 2 Tc1 Tc1 NNP work_sny56posuffadh5a367n5vi4uq 303 3 / / SYM work_sny56posuffadh5a367n5vi4uq 303 4 mariner mariner NNP work_sny56posuffadh5a367n5vi4uq 303 5 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 303 6 family family NN work_sny56posuffadh5a367n5vi4uq 303 7 . . . work_sny56posuffadh5a367n5vi4uq 304 1 Curr Curr NNP work_sny56posuffadh5a367n5vi4uq 304 2 Top Top NNP work_sny56posuffadh5a367n5vi4uq 304 3 Microbiol Microbiol NNP work_sny56posuffadh5a367n5vi4uq 304 4 Immunol Immunol NNP work_sny56posuffadh5a367n5vi4uq 304 5 1996 1996 CD work_sny56posuffadh5a367n5vi4uq 304 6 ; ; : work_sny56posuffadh5a367n5vi4uq 304 7 204 204 CD work_sny56posuffadh5a367n5vi4uq 304 8 : : : work_sny56posuffadh5a367n5vi4uq 304 9 125 125 CD work_sny56posuffadh5a367n5vi4uq 304 10 – – : work_sny56posuffadh5a367n5vi4uq 304 11 143 143 CD work_sny56posuffadh5a367n5vi4uq 304 12 . . . work_sny56posuffadh5a367n5vi4uq 305 1 2 2 LS work_sny56posuffadh5a367n5vi4uq 305 2 . . . work_sny56posuffadh5a367n5vi4uq 306 1 Plasterk Plasterk NNP work_sny56posuffadh5a367n5vi4uq 306 2 RH RH NNP work_sny56posuffadh5a367n5vi4uq 306 3 , , , work_sny56posuffadh5a367n5vi4uq 306 4 Izsvak Izsvak NNP work_sny56posuffadh5a367n5vi4uq 306 5 Z Z NNP work_sny56posuffadh5a367n5vi4uq 306 6 , , , work_sny56posuffadh5a367n5vi4uq 306 7 Ivics Ivics NNP work_sny56posuffadh5a367n5vi4uq 306 8 Z. Z. NNP work_sny56posuffadh5a367n5vi4uq 306 9 Resident Resident NNP work_sny56posuffadh5a367n5vi4uq 306 10 aliens alien NNS work_sny56posuffadh5a367n5vi4uq 306 11 : : : work_sny56posuffadh5a367n5vi4uq 306 12 the the DT work_sny56posuffadh5a367n5vi4uq 306 13 Tc1 Tc1 NNP work_sny56posuffadh5a367n5vi4uq 306 14 / / SYM work_sny56posuffadh5a367n5vi4uq 306 15 mariner mariner NNP work_sny56posuffadh5a367n5vi4uq 306 16 superfamily superfamily RB work_sny56posuffadh5a367n5vi4uq 306 17 of of IN work_sny56posuffadh5a367n5vi4uq 306 18 transposable transposable JJ work_sny56posuffadh5a367n5vi4uq 306 19 elements element NNS work_sny56posuffadh5a367n5vi4uq 306 20 . . . work_sny56posuffadh5a367n5vi4uq 307 1 Trends Trends NNPS work_sny56posuffadh5a367n5vi4uq 307 2 Genet Genet NNP work_sny56posuffadh5a367n5vi4uq 307 3 1999 1999 CD work_sny56posuffadh5a367n5vi4uq 307 4 ; ; : work_sny56posuffadh5a367n5vi4uq 307 5 15 15 CD work_sny56posuffadh5a367n5vi4uq 307 6 : : SYM work_sny56posuffadh5a367n5vi4uq 307 7 326 326 CD work_sny56posuffadh5a367n5vi4uq 307 8 – – : work_sny56posuffadh5a367n5vi4uq 307 9 332 332 CD work_sny56posuffadh5a367n5vi4uq 307 10 . . . work_sny56posuffadh5a367n5vi4uq 308 1 3 3 LS work_sny56posuffadh5a367n5vi4uq 308 2 . . . work_sny56posuffadh5a367n5vi4uq 309 1 Ivics Ivics NNP work_sny56posuffadh5a367n5vi4uq 309 2 Z Z NNP work_sny56posuffadh5a367n5vi4uq 309 3 , , , work_sny56posuffadh5a367n5vi4uq 309 4 Hackett Hackett NNP work_sny56posuffadh5a367n5vi4uq 309 5 PB PB NNP work_sny56posuffadh5a367n5vi4uq 309 6 , , , work_sny56posuffadh5a367n5vi4uq 309 7 Plasterk Plasterk NNP work_sny56posuffadh5a367n5vi4uq 309 8 RH RH NNP work_sny56posuffadh5a367n5vi4uq 309 9 , , , work_sny56posuffadh5a367n5vi4uq 309 10 Izsvak Izsvak NNP work_sny56posuffadh5a367n5vi4uq 309 11 Z. Z. NNP work_sny56posuffadh5a367n5vi4uq 309 12 Molecular Molecular NNP work_sny56posuffadh5a367n5vi4uq 309 13 reconstruction reconstruction NN work_sny56posuffadh5a367n5vi4uq 309 14 of of IN work_sny56posuffadh5a367n5vi4uq 309 15 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 309 16 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 309 17 , , , work_sny56posuffadh5a367n5vi4uq 309 18 a a DT work_sny56posuffadh5a367n5vi4uq 309 19 Tc1-like tc1-like JJ work_sny56posuffadh5a367n5vi4uq 309 20 transposon transposon NN work_sny56posuffadh5a367n5vi4uq 309 21 from from IN work_sny56posuffadh5a367n5vi4uq 309 22 fish fish NN work_sny56posuffadh5a367n5vi4uq 309 23 , , , work_sny56posuffadh5a367n5vi4uq 309 24 and and CC work_sny56posuffadh5a367n5vi4uq 309 25 its -PRON- PRP$ work_sny56posuffadh5a367n5vi4uq 309 26 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 309 27 in in IN work_sny56posuffadh5a367n5vi4uq 309 28 human human JJ work_sny56posuffadh5a367n5vi4uq 309 29 cells cell NNS work_sny56posuffadh5a367n5vi4uq 309 30 . . . work_sny56posuffadh5a367n5vi4uq 310 1 Cell cell NN work_sny56posuffadh5a367n5vi4uq 310 2 1997 1997 CD work_sny56posuffadh5a367n5vi4uq 310 3 ; ; : work_sny56posuffadh5a367n5vi4uq 310 4 91 91 CD work_sny56posuffadh5a367n5vi4uq 310 5 : : : work_sny56posuffadh5a367n5vi4uq 310 6 501 501 CD work_sny56posuffadh5a367n5vi4uq 310 7 – – : work_sny56posuffadh5a367n5vi4uq 310 8 510 510 CD work_sny56posuffadh5a367n5vi4uq 310 9 . . . work_sny56posuffadh5a367n5vi4uq 311 1 4 4 LS work_sny56posuffadh5a367n5vi4uq 311 2 . . . work_sny56posuffadh5a367n5vi4uq 312 1 Fischer Fischer NNP work_sny56posuffadh5a367n5vi4uq 312 2 SE SE NNP work_sny56posuffadh5a367n5vi4uq 312 3 , , , work_sny56posuffadh5a367n5vi4uq 312 4 Wienholds Wienholds NNP work_sny56posuffadh5a367n5vi4uq 312 5 E E NNP work_sny56posuffadh5a367n5vi4uq 312 6 , , , work_sny56posuffadh5a367n5vi4uq 312 7 Plasterk Plasterk NNP work_sny56posuffadh5a367n5vi4uq 312 8 RH RH NNP work_sny56posuffadh5a367n5vi4uq 312 9 . . . work_sny56posuffadh5a367n5vi4uq 313 1 Regulated regulated JJ work_sny56posuffadh5a367n5vi4uq 313 2 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 313 3 of of IN work_sny56posuffadh5a367n5vi4uq 313 4 a a DT work_sny56posuffadh5a367n5vi4uq 313 5 fish fish NN work_sny56posuffadh5a367n5vi4uq 313 6 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 313 7 in in IN work_sny56posuffadh5a367n5vi4uq 313 8 the the DT work_sny56posuffadh5a367n5vi4uq 313 9 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 313 10 germ germ NN work_sny56posuffadh5a367n5vi4uq 313 11 line line NN work_sny56posuffadh5a367n5vi4uq 313 12 . . . work_sny56posuffadh5a367n5vi4uq 314 1 Proc Proc NNP work_sny56posuffadh5a367n5vi4uq 314 2 Natl Natl NNP work_sny56posuffadh5a367n5vi4uq 314 3 Acad Acad NNP work_sny56posuffadh5a367n5vi4uq 314 4 Sci Sci NNP work_sny56posuffadh5a367n5vi4uq 314 5 U U NNP work_sny56posuffadh5a367n5vi4uq 314 6 S S NNP work_sny56posuffadh5a367n5vi4uq 314 7 A A NNP work_sny56posuffadh5a367n5vi4uq 314 8 2001 2001 CD work_sny56posuffadh5a367n5vi4uq 314 9 ; ; : work_sny56posuffadh5a367n5vi4uq 314 10 98 98 CD work_sny56posuffadh5a367n5vi4uq 314 11 : : SYM work_sny56posuffadh5a367n5vi4uq 314 12 6759 6759 CD work_sny56posuffadh5a367n5vi4uq 314 13 – – : work_sny56posuffadh5a367n5vi4uq 314 14 6764 6764 CD work_sny56posuffadh5a367n5vi4uq 314 15 . . . work_sny56posuffadh5a367n5vi4uq 315 1 5 5 CD work_sny56posuffadh5a367n5vi4uq 315 2 . . . work_sny56posuffadh5a367n5vi4uq 316 1 Izsvak Izsvak NNP work_sny56posuffadh5a367n5vi4uq 316 2 Z Z NNP work_sny56posuffadh5a367n5vi4uq 316 3 , , , work_sny56posuffadh5a367n5vi4uq 316 4 Ivics Ivics NNP work_sny56posuffadh5a367n5vi4uq 316 5 Z Z NNP work_sny56posuffadh5a367n5vi4uq 316 6 , , , work_sny56posuffadh5a367n5vi4uq 316 7 Plasterk Plasterk NNP work_sny56posuffadh5a367n5vi4uq 316 8 RH RH NNP work_sny56posuffadh5a367n5vi4uq 316 9 . . . work_sny56posuffadh5a367n5vi4uq 317 1 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 317 2 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 317 3 , , , work_sny56posuffadh5a367n5vi4uq 317 4 a a DT work_sny56posuffadh5a367n5vi4uq 317 5 wide wide JJ work_sny56posuffadh5a367n5vi4uq 317 6 host host NN work_sny56posuffadh5a367n5vi4uq 317 7 - - JJ work_sny56posuffadh5a367n5vi4uq 317 8 range range JJ work_sny56posuffadh5a367n5vi4uq 317 9 transposon transposon JJ work_sny56posuffadh5a367n5vi4uq 317 10 vector vector NN work_sny56posuffadh5a367n5vi4uq 317 11 for for IN work_sny56posuffadh5a367n5vi4uq 317 12 genetic genetic JJ work_sny56posuffadh5a367n5vi4uq 317 13 transformation transformation NN work_sny56posuffadh5a367n5vi4uq 317 14 in in IN work_sny56posuffadh5a367n5vi4uq 317 15 vertebrates vertebrate NNS work_sny56posuffadh5a367n5vi4uq 317 16 . . . work_sny56posuffadh5a367n5vi4uq 318 1 J J NNP work_sny56posuffadh5a367n5vi4uq 318 2 Mol Mol NNP work_sny56posuffadh5a367n5vi4uq 318 3 Biol Biol NNP work_sny56posuffadh5a367n5vi4uq 318 4 2000 2000 CD work_sny56posuffadh5a367n5vi4uq 318 5 ; ; : work_sny56posuffadh5a367n5vi4uq 318 6 302 302 CD work_sny56posuffadh5a367n5vi4uq 318 7 : : : work_sny56posuffadh5a367n5vi4uq 318 8 93 93 CD work_sny56posuffadh5a367n5vi4uq 318 9 – – : work_sny56posuffadh5a367n5vi4uq 318 10 102 102 CD work_sny56posuffadh5a367n5vi4uq 318 11 . . . work_sny56posuffadh5a367n5vi4uq 319 1 6 6 CD work_sny56posuffadh5a367n5vi4uq 319 2 . . . work_sny56posuffadh5a367n5vi4uq 320 1 Dupuy Dupuy NNP work_sny56posuffadh5a367n5vi4uq 320 2 AJ AJ NNP work_sny56posuffadh5a367n5vi4uq 320 3 , , , work_sny56posuffadh5a367n5vi4uq 320 4 Fritz Fritz NNP work_sny56posuffadh5a367n5vi4uq 320 5 S S NNP work_sny56posuffadh5a367n5vi4uq 320 6 , , , work_sny56posuffadh5a367n5vi4uq 320 7 Largaespada Largaespada NNP work_sny56posuffadh5a367n5vi4uq 320 8 DA DA NNP work_sny56posuffadh5a367n5vi4uq 320 9 . . . work_sny56posuffadh5a367n5vi4uq 321 1 Transposition transposition NN work_sny56posuffadh5a367n5vi4uq 321 2 and and CC work_sny56posuffadh5a367n5vi4uq 321 3 gene gene NN work_sny56posuffadh5a367n5vi4uq 321 4 disruption disruption NN work_sny56posuffadh5a367n5vi4uq 321 5 in in IN work_sny56posuffadh5a367n5vi4uq 321 6 the the DT work_sny56posuffadh5a367n5vi4uq 321 7 male male JJ work_sny56posuffadh5a367n5vi4uq 321 8 germline germline NN work_sny56posuffadh5a367n5vi4uq 321 9 of of IN work_sny56posuffadh5a367n5vi4uq 321 10 the the DT work_sny56posuffadh5a367n5vi4uq 321 11 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 321 12 . . . work_sny56posuffadh5a367n5vi4uq 322 1 Genesis Genesis NNP work_sny56posuffadh5a367n5vi4uq 322 2 2001 2001 CD work_sny56posuffadh5a367n5vi4uq 322 3 ; ; : work_sny56posuffadh5a367n5vi4uq 322 4 30 30 CD work_sny56posuffadh5a367n5vi4uq 322 5 : : : work_sny56posuffadh5a367n5vi4uq 322 6 82 82 CD work_sny56posuffadh5a367n5vi4uq 322 7 – – SYM work_sny56posuffadh5a367n5vi4uq 322 8 88 88 CD work_sny56posuffadh5a367n5vi4uq 322 9 . . . work_sny56posuffadh5a367n5vi4uq 323 1 7 7 LS work_sny56posuffadh5a367n5vi4uq 323 2 . . . work_sny56posuffadh5a367n5vi4uq 324 1 Horie Horie NNP work_sny56posuffadh5a367n5vi4uq 324 2 K K NNP work_sny56posuffadh5a367n5vi4uq 324 3 , , , work_sny56posuffadh5a367n5vi4uq 324 4 Kuroiwa Kuroiwa NNPS work_sny56posuffadh5a367n5vi4uq 324 5 A A NNP work_sny56posuffadh5a367n5vi4uq 324 6 , , , work_sny56posuffadh5a367n5vi4uq 324 7 Ikawa Ikawa NNP work_sny56posuffadh5a367n5vi4uq 324 8 M M NNP work_sny56posuffadh5a367n5vi4uq 324 9 , , , work_sny56posuffadh5a367n5vi4uq 324 10 et et NNP work_sny56posuffadh5a367n5vi4uq 324 11 al al NNP work_sny56posuffadh5a367n5vi4uq 324 12 . . . work_sny56posuffadh5a367n5vi4uq 325 1 Efficient efficient JJ work_sny56posuffadh5a367n5vi4uq 325 2 chromosomal chromosomal JJ work_sny56posuffadh5a367n5vi4uq 325 3 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 325 4 of of IN work_sny56posuffadh5a367n5vi4uq 325 5 a a DT work_sny56posuffadh5a367n5vi4uq 325 6 Tc1 Tc1 NNP work_sny56posuffadh5a367n5vi4uq 325 7 / / SYM work_sny56posuffadh5a367n5vi4uq 325 8 mariner mariner NN work_sny56posuffadh5a367n5vi4uq 325 9 - - HYPH work_sny56posuffadh5a367n5vi4uq 325 10 like like JJ work_sny56posuffadh5a367n5vi4uq 325 11 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 325 12 Sleeping sleep VBG work_sny56posuffadh5a367n5vi4uq 325 13 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 325 14 in in IN work_sny56posuffadh5a367n5vi4uq 325 15 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 325 16 . . . work_sny56posuffadh5a367n5vi4uq 326 1 Proc Proc NNP work_sny56posuffadh5a367n5vi4uq 326 2 Natl Natl NNP work_sny56posuffadh5a367n5vi4uq 326 3 Acad Acad NNP work_sny56posuffadh5a367n5vi4uq 326 4 Sci Sci NNP work_sny56posuffadh5a367n5vi4uq 326 5 U U NNP work_sny56posuffadh5a367n5vi4uq 326 6 S S NNP work_sny56posuffadh5a367n5vi4uq 326 7 A A NNP work_sny56posuffadh5a367n5vi4uq 326 8 2001 2001 CD work_sny56posuffadh5a367n5vi4uq 326 9 ; ; : work_sny56posuffadh5a367n5vi4uq 326 10 98 98 CD work_sny56posuffadh5a367n5vi4uq 326 11 : : SYM work_sny56posuffadh5a367n5vi4uq 326 12 9191 9191 CD work_sny56posuffadh5a367n5vi4uq 326 13 – – : work_sny56posuffadh5a367n5vi4uq 326 14 9196 9196 CD work_sny56posuffadh5a367n5vi4uq 326 15 . . . work_sny56posuffadh5a367n5vi4uq 327 1 8 8 LS work_sny56posuffadh5a367n5vi4uq 327 2 . . . work_sny56posuffadh5a367n5vi4uq 328 1 Clark Clark NNP work_sny56posuffadh5a367n5vi4uq 328 2 KJ KJ NNP work_sny56posuffadh5a367n5vi4uq 328 3 , , , work_sny56posuffadh5a367n5vi4uq 328 4 Geurts Geurts NNP work_sny56posuffadh5a367n5vi4uq 328 5 AM AM NNP work_sny56posuffadh5a367n5vi4uq 328 6 , , , work_sny56posuffadh5a367n5vi4uq 328 7 Bell Bell NNP work_sny56posuffadh5a367n5vi4uq 328 8 JB JB NNP work_sny56posuffadh5a367n5vi4uq 328 9 , , , work_sny56posuffadh5a367n5vi4uq 328 10 Hackett Hackett NNP work_sny56posuffadh5a367n5vi4uq 328 11 PB PB NNP work_sny56posuffadh5a367n5vi4uq 328 12 . . . work_sny56posuffadh5a367n5vi4uq 329 1 Transposon Transposon NNP work_sny56posuffadh5a367n5vi4uq 329 2 vectors vector NNS work_sny56posuffadh5a367n5vi4uq 329 3 for for IN work_sny56posuffadh5a367n5vi4uq 329 4 gene gene NN work_sny56posuffadh5a367n5vi4uq 329 5 - - HYPH work_sny56posuffadh5a367n5vi4uq 329 6 trap trap NN work_sny56posuffadh5a367n5vi4uq 329 7 insertional insertional JJ work_sny56posuffadh5a367n5vi4uq 329 8 mutagenesis mutagenesis NN work_sny56posuffadh5a367n5vi4uq 329 9 in in IN work_sny56posuffadh5a367n5vi4uq 329 10 vertebrates vertebrate NNS work_sny56posuffadh5a367n5vi4uq 329 11 . . . work_sny56posuffadh5a367n5vi4uq 330 1 Proc Proc NNP work_sny56posuffadh5a367n5vi4uq 330 2 Natl Natl NNP work_sny56posuffadh5a367n5vi4uq 330 3 Acd Acd NNP work_sny56posuffadh5a367n5vi4uq 330 4 Sci Sci NNP work_sny56posuffadh5a367n5vi4uq 330 5 U U NNP work_sny56posuffadh5a367n5vi4uq 330 6 S S NNP work_sny56posuffadh5a367n5vi4uq 330 7 A A NNP work_sny56posuffadh5a367n5vi4uq 330 8 2003 2003 CD work_sny56posuffadh5a367n5vi4uq 330 9 ; ; : work_sny56posuffadh5a367n5vi4uq 330 10 submitted submit VBN work_sny56posuffadh5a367n5vi4uq 330 11 . . . work_sny56posuffadh5a367n5vi4uq 331 1 9 9 CD work_sny56posuffadh5a367n5vi4uq 331 2 . . . work_sny56posuffadh5a367n5vi4uq 332 1 Dupuy Dupuy NNP work_sny56posuffadh5a367n5vi4uq 332 2 AJ AJ NNP work_sny56posuffadh5a367n5vi4uq 332 3 , , , work_sny56posuffadh5a367n5vi4uq 332 4 Clark Clark NNP work_sny56posuffadh5a367n5vi4uq 332 5 KJ KJ NNP work_sny56posuffadh5a367n5vi4uq 332 6 , , , work_sny56posuffadh5a367n5vi4uq 332 7 Carlson Carlson NNP work_sny56posuffadh5a367n5vi4uq 332 8 CM CM NNP work_sny56posuffadh5a367n5vi4uq 332 9 , , , work_sny56posuffadh5a367n5vi4uq 332 10 et et NNP work_sny56posuffadh5a367n5vi4uq 332 11 al al NNP work_sny56posuffadh5a367n5vi4uq 332 12 . . . work_sny56posuffadh5a367n5vi4uq 333 1 Mammalian mammalian JJ work_sny56posuffadh5a367n5vi4uq 333 2 germ germ NN work_sny56posuffadh5a367n5vi4uq 333 3 - - HYPH work_sny56posuffadh5a367n5vi4uq 333 4 line line NN work_sny56posuffadh5a367n5vi4uq 333 5 transgenesis transgenesis NN work_sny56posuffadh5a367n5vi4uq 333 6 by by IN work_sny56posuffadh5a367n5vi4uq 333 7 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 333 8 . . . work_sny56posuffadh5a367n5vi4uq 334 1 Proc Proc NNP work_sny56posuffadh5a367n5vi4uq 334 2 Natl Natl NNP work_sny56posuffadh5a367n5vi4uq 334 3 Acad Acad NNP work_sny56posuffadh5a367n5vi4uq 334 4 Sci Sci NNP work_sny56posuffadh5a367n5vi4uq 334 5 U U NNP work_sny56posuffadh5a367n5vi4uq 334 6 S S NNP work_sny56posuffadh5a367n5vi4uq 334 7 A A NNP work_sny56posuffadh5a367n5vi4uq 334 8 2002 2002 CD work_sny56posuffadh5a367n5vi4uq 334 9 ; ; : work_sny56posuffadh5a367n5vi4uq 334 10 99 99 CD work_sny56posuffadh5a367n5vi4uq 334 11 : : : work_sny56posuffadh5a367n5vi4uq 334 12 4495 4495 CD work_sny56posuffadh5a367n5vi4uq 334 13 – – : work_sny56posuffadh5a367n5vi4uq 334 14 4499 4499 CD work_sny56posuffadh5a367n5vi4uq 334 15 . . . work_sny56posuffadh5a367n5vi4uq 335 1 10 10 CD work_sny56posuffadh5a367n5vi4uq 335 2 . . . work_sny56posuffadh5a367n5vi4uq 336 1 Yant yant JJ work_sny56posuffadh5a367n5vi4uq 336 2 SR SR NNP work_sny56posuffadh5a367n5vi4uq 336 3 , , , work_sny56posuffadh5a367n5vi4uq 336 4 Meuse Meuse NNP work_sny56posuffadh5a367n5vi4uq 336 5 L L NNP work_sny56posuffadh5a367n5vi4uq 336 6 , , , work_sny56posuffadh5a367n5vi4uq 336 7 Chiu Chiu NNP work_sny56posuffadh5a367n5vi4uq 336 8 W W NNP work_sny56posuffadh5a367n5vi4uq 336 9 , , , work_sny56posuffadh5a367n5vi4uq 336 10 et et NNP work_sny56posuffadh5a367n5vi4uq 336 11 al al NNP work_sny56posuffadh5a367n5vi4uq 336 12 . . . work_sny56posuffadh5a367n5vi4uq 337 1 Somatic somatic JJ work_sny56posuffadh5a367n5vi4uq 337 2 integration integration NN work_sny56posuffadh5a367n5vi4uq 337 3 and and CC work_sny56posuffadh5a367n5vi4uq 337 4 long- long- JJ work_sny56posuffadh5a367n5vi4uq 337 5 term term NN work_sny56posuffadh5a367n5vi4uq 337 6 transgene transgene JJ work_sny56posuffadh5a367n5vi4uq 337 7 expression expression NN work_sny56posuffadh5a367n5vi4uq 337 8 in in IN work_sny56posuffadh5a367n5vi4uq 337 9 normal normal JJ work_sny56posuffadh5a367n5vi4uq 337 10 and and CC work_sny56posuffadh5a367n5vi4uq 337 11 haemophilic haemophilic JJ work_sny56posuffadh5a367n5vi4uq 337 12 mice mouse NNS work_sny56posuffadh5a367n5vi4uq 337 13 using use VBG work_sny56posuffadh5a367n5vi4uq 337 14 a a DT work_sny56posuffadh5a367n5vi4uq 337 15 DNA dna NN work_sny56posuffadh5a367n5vi4uq 337 16 transposon transposon NN work_sny56posuffadh5a367n5vi4uq 337 17 system system NN work_sny56posuffadh5a367n5vi4uq 337 18 . . . work_sny56posuffadh5a367n5vi4uq 338 1 Nat Nat NNP work_sny56posuffadh5a367n5vi4uq 338 2 Genet Genet NNP work_sny56posuffadh5a367n5vi4uq 338 3 2000 2000 CD work_sny56posuffadh5a367n5vi4uq 338 4 ; ; : work_sny56posuffadh5a367n5vi4uq 338 5 25 25 CD work_sny56posuffadh5a367n5vi4uq 338 6 : : SYM work_sny56posuffadh5a367n5vi4uq 338 7 35 35 CD work_sny56posuffadh5a367n5vi4uq 338 8 – – : work_sny56posuffadh5a367n5vi4uq 338 9 41 41 CD work_sny56posuffadh5a367n5vi4uq 338 10 . . . work_sny56posuffadh5a367n5vi4uq 339 1 11 11 CD work_sny56posuffadh5a367n5vi4uq 339 2 . . . work_sny56posuffadh5a367n5vi4uq 340 1 Yant yant JJ work_sny56posuffadh5a367n5vi4uq 340 2 SR SR NNP work_sny56posuffadh5a367n5vi4uq 340 3 , , , work_sny56posuffadh5a367n5vi4uq 340 4 Ehrhardt Ehrhardt NNP work_sny56posuffadh5a367n5vi4uq 340 5 A A NNP work_sny56posuffadh5a367n5vi4uq 340 6 , , , work_sny56posuffadh5a367n5vi4uq 340 7 Mikkelsen Mikkelsen NNP work_sny56posuffadh5a367n5vi4uq 340 8 JG JG NNP work_sny56posuffadh5a367n5vi4uq 340 9 , , , work_sny56posuffadh5a367n5vi4uq 340 10 et et NNP work_sny56posuffadh5a367n5vi4uq 340 11 al al NNP work_sny56posuffadh5a367n5vi4uq 340 12 . . . work_sny56posuffadh5a367n5vi4uq 341 1 Transposition transposition NN work_sny56posuffadh5a367n5vi4uq 341 2 from from IN work_sny56posuffadh5a367n5vi4uq 341 3 a a DT work_sny56posuffadh5a367n5vi4uq 341 4 gutless gutless JJ work_sny56posuffadh5a367n5vi4uq 341 5 adenotransposon adenotransposon JJ work_sny56posuffadh5a367n5vi4uq 341 6 vector vector NN work_sny56posuffadh5a367n5vi4uq 341 7 stabilizes stabilize VBZ work_sny56posuffadh5a367n5vi4uq 341 8 transgene transgene JJ work_sny56posuffadh5a367n5vi4uq 341 9 expression expression NN work_sny56posuffadh5a367n5vi4uq 341 10 in in IN work_sny56posuffadh5a367n5vi4uq 341 11 vivo vivo NNP work_sny56posuffadh5a367n5vi4uq 341 12 . . . work_sny56posuffadh5a367n5vi4uq 342 1 Nat Nat NNP work_sny56posuffadh5a367n5vi4uq 342 2 Biotechnol Biotechnol NNP work_sny56posuffadh5a367n5vi4uq 342 3 2002 2002 CD work_sny56posuffadh5a367n5vi4uq 342 4 ; ; : work_sny56posuffadh5a367n5vi4uq 342 5 20 20 CD work_sny56posuffadh5a367n5vi4uq 342 6 : : SYM work_sny56posuffadh5a367n5vi4uq 342 7 999 999 CD work_sny56posuffadh5a367n5vi4uq 342 8 – – SYM work_sny56posuffadh5a367n5vi4uq 342 9 1005 1005 CD work_sny56posuffadh5a367n5vi4uq 342 10 . . . work_sny56posuffadh5a367n5vi4uq 343 1 12 12 CD work_sny56posuffadh5a367n5vi4uq 343 2 . . . work_sny56posuffadh5a367n5vi4uq 344 1 Belur Belur NNP work_sny56posuffadh5a367n5vi4uq 344 2 LR LR NNP work_sny56posuffadh5a367n5vi4uq 344 3 , , , work_sny56posuffadh5a367n5vi4uq 344 4 Frandsen Frandsen NNP work_sny56posuffadh5a367n5vi4uq 344 5 JL JL NNP work_sny56posuffadh5a367n5vi4uq 344 6 , , , work_sny56posuffadh5a367n5vi4uq 344 7 Dupuy Dupuy NNP work_sny56posuffadh5a367n5vi4uq 344 8 AJ AJ NNP work_sny56posuffadh5a367n5vi4uq 344 9 , , , work_sny56posuffadh5a367n5vi4uq 344 10 et et NNP work_sny56posuffadh5a367n5vi4uq 344 11 al al NNP work_sny56posuffadh5a367n5vi4uq 344 12 . . . work_sny56posuffadh5a367n5vi4uq 345 1 Gene gene NN work_sny56posuffadh5a367n5vi4uq 345 2 insertion insertion NN work_sny56posuffadh5a367n5vi4uq 345 3 and and CC work_sny56posuffadh5a367n5vi4uq 345 4 long long JJ work_sny56posuffadh5a367n5vi4uq 345 5 - - HYPH work_sny56posuffadh5a367n5vi4uq 345 6 term term NN work_sny56posuffadh5a367n5vi4uq 345 7 expression expression NN work_sny56posuffadh5a367n5vi4uq 345 8 in in IN work_sny56posuffadh5a367n5vi4uq 345 9 lung lung NN work_sny56posuffadh5a367n5vi4uq 345 10 mediated mediate VBN work_sny56posuffadh5a367n5vi4uq 345 11 by by IN work_sny56posuffadh5a367n5vi4uq 345 12 the the DT work_sny56posuffadh5a367n5vi4uq 345 13 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 345 14 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 345 15 transposon transposon NN work_sny56posuffadh5a367n5vi4uq 345 16 system system NN work_sny56posuffadh5a367n5vi4uq 345 17 . . . work_sny56posuffadh5a367n5vi4uq 346 1 Mol Mol NNP work_sny56posuffadh5a367n5vi4uq 346 2 Ther Ther NNP work_sny56posuffadh5a367n5vi4uq 346 3 2003 2003 CD work_sny56posuffadh5a367n5vi4uq 346 4 ; ; : work_sny56posuffadh5a367n5vi4uq 346 5 8 8 CD work_sny56posuffadh5a367n5vi4uq 346 6 : : : work_sny56posuffadh5a367n5vi4uq 346 7 501 501 CD work_sny56posuffadh5a367n5vi4uq 346 8 – – : work_sny56posuffadh5a367n5vi4uq 346 9 507 507 CD work_sny56posuffadh5a367n5vi4uq 346 10 . . . work_sny56posuffadh5a367n5vi4uq 347 1 13 13 CD work_sny56posuffadh5a367n5vi4uq 347 2 . . . work_sny56posuffadh5a367n5vi4uq 348 1 Geurts geurt NNS work_sny56posuffadh5a367n5vi4uq 348 2 AM am NN work_sny56posuffadh5a367n5vi4uq 348 3 , , , work_sny56posuffadh5a367n5vi4uq 348 4 Yang Yang NNP work_sny56posuffadh5a367n5vi4uq 348 5 Y Y NNP work_sny56posuffadh5a367n5vi4uq 348 6 , , , work_sny56posuffadh5a367n5vi4uq 348 7 Clark Clark NNP work_sny56posuffadh5a367n5vi4uq 348 8 KJ KJ NNP work_sny56posuffadh5a367n5vi4uq 348 9 , , , work_sny56posuffadh5a367n5vi4uq 348 10 et et NNP work_sny56posuffadh5a367n5vi4uq 348 11 al al NNP work_sny56posuffadh5a367n5vi4uq 348 12 . . . work_sny56posuffadh5a367n5vi4uq 349 1 Gene gene NN work_sny56posuffadh5a367n5vi4uq 349 2 transfer transfer NN work_sny56posuffadh5a367n5vi4uq 349 3 into into IN work_sny56posuffadh5a367n5vi4uq 349 4 genomes genome NNS work_sny56posuffadh5a367n5vi4uq 349 5 of of IN work_sny56posuffadh5a367n5vi4uq 349 6 human human JJ work_sny56posuffadh5a367n5vi4uq 349 7 cells cell NNS work_sny56posuffadh5a367n5vi4uq 349 8 by by IN work_sny56posuffadh5a367n5vi4uq 349 9 the the DT work_sny56posuffadh5a367n5vi4uq 349 10 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 349 11 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 349 12 transposon transposon NN work_sny56posuffadh5a367n5vi4uq 349 13 system system NN work_sny56posuffadh5a367n5vi4uq 349 14 . . . work_sny56posuffadh5a367n5vi4uq 350 1 Mol Mol NNP work_sny56posuffadh5a367n5vi4uq 350 2 Ther Ther NNP work_sny56posuffadh5a367n5vi4uq 350 3 2003 2003 CD work_sny56posuffadh5a367n5vi4uq 350 4 ; ; : work_sny56posuffadh5a367n5vi4uq 350 5 8 8 CD work_sny56posuffadh5a367n5vi4uq 350 6 : : SYM work_sny56posuffadh5a367n5vi4uq 350 7 108 108 CD work_sny56posuffadh5a367n5vi4uq 350 8 – – SYM work_sny56posuffadh5a367n5vi4uq 350 9 117 117 CD work_sny56posuffadh5a367n5vi4uq 350 10 . . . work_sny56posuffadh5a367n5vi4uq 351 1 14 14 CD work_sny56posuffadh5a367n5vi4uq 351 2 . . . work_sny56posuffadh5a367n5vi4uq 352 1 Luo Luo NNP work_sny56posuffadh5a367n5vi4uq 352 2 G G NNP work_sny56posuffadh5a367n5vi4uq 352 3 , , , work_sny56posuffadh5a367n5vi4uq 352 4 Ivics Ivics NNP work_sny56posuffadh5a367n5vi4uq 352 5 Z Z NNP work_sny56posuffadh5a367n5vi4uq 352 6 , , , work_sny56posuffadh5a367n5vi4uq 352 7 Izsvak Izsvak NNP work_sny56posuffadh5a367n5vi4uq 352 8 Z Z NNP work_sny56posuffadh5a367n5vi4uq 352 9 , , , work_sny56posuffadh5a367n5vi4uq 352 10 Bradley Bradley NNP work_sny56posuffadh5a367n5vi4uq 352 11 A. A. NNP work_sny56posuffadh5a367n5vi4uq 352 12 Chromosomal Chromosomal NNP work_sny56posuffadh5a367n5vi4uq 352 13 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 352 14 of of IN work_sny56posuffadh5a367n5vi4uq 352 15 a a DT work_sny56posuffadh5a367n5vi4uq 352 16 Tc1 tc1 NN work_sny56posuffadh5a367n5vi4uq 352 17 / / SYM work_sny56posuffadh5a367n5vi4uq 352 18 mariner mariner NN work_sny56posuffadh5a367n5vi4uq 352 19 - - HYPH work_sny56posuffadh5a367n5vi4uq 352 20 like like JJ work_sny56posuffadh5a367n5vi4uq 352 21 element element NN work_sny56posuffadh5a367n5vi4uq 352 22 in in IN work_sny56posuffadh5a367n5vi4uq 352 23 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 352 24 embryonic embryonic JJ work_sny56posuffadh5a367n5vi4uq 352 25 stem stem NN work_sny56posuffadh5a367n5vi4uq 352 26 cells cell NNS work_sny56posuffadh5a367n5vi4uq 352 27 . . . work_sny56posuffadh5a367n5vi4uq 353 1 Proc Proc NNP work_sny56posuffadh5a367n5vi4uq 353 2 Natl Natl NNP work_sny56posuffadh5a367n5vi4uq 353 3 Acad Acad NNP work_sny56posuffadh5a367n5vi4uq 353 4 Sci Sci NNP work_sny56posuffadh5a367n5vi4uq 353 5 U U NNP work_sny56posuffadh5a367n5vi4uq 353 6 S S NNP work_sny56posuffadh5a367n5vi4uq 353 7 A A NNP work_sny56posuffadh5a367n5vi4uq 353 8 1998 1998 CD work_sny56posuffadh5a367n5vi4uq 353 9 ; ; : work_sny56posuffadh5a367n5vi4uq 353 10 95 95 CD work_sny56posuffadh5a367n5vi4uq 353 11 : : SYM work_sny56posuffadh5a367n5vi4uq 353 12 10 10 CD work_sny56posuffadh5a367n5vi4uq 353 13 769 769 CD work_sny56posuffadh5a367n5vi4uq 353 14 – – : work_sny56posuffadh5a367n5vi4uq 353 15 10 10 CD work_sny56posuffadh5a367n5vi4uq 353 16 773 773 CD work_sny56posuffadh5a367n5vi4uq 353 17 . . . work_sny56posuffadh5a367n5vi4uq 354 1 15 15 CD work_sny56posuffadh5a367n5vi4uq 354 2 . . . work_sny56posuffadh5a367n5vi4uq 355 1 Vigdal Vigdal NNP work_sny56posuffadh5a367n5vi4uq 355 2 TJ TJ NNP work_sny56posuffadh5a367n5vi4uq 355 3 , , , work_sny56posuffadh5a367n5vi4uq 355 4 Kaufman Kaufman NNP work_sny56posuffadh5a367n5vi4uq 355 5 CD CD NNP work_sny56posuffadh5a367n5vi4uq 355 6 , , , work_sny56posuffadh5a367n5vi4uq 355 7 Izsvak Izsvak NNP work_sny56posuffadh5a367n5vi4uq 355 8 Z Z NNP work_sny56posuffadh5a367n5vi4uq 355 9 , , , work_sny56posuffadh5a367n5vi4uq 355 10 et et NNP work_sny56posuffadh5a367n5vi4uq 355 11 al al NNP work_sny56posuffadh5a367n5vi4uq 355 12 . . . work_sny56posuffadh5a367n5vi4uq 356 1 Common common JJ work_sny56posuffadh5a367n5vi4uq 356 2 physical physical JJ work_sny56posuffadh5a367n5vi4uq 356 3 properties property NNS work_sny56posuffadh5a367n5vi4uq 356 4 of of IN work_sny56posuffadh5a367n5vi4uq 356 5 DNA dna NN work_sny56posuffadh5a367n5vi4uq 356 6 affecting affect VBG work_sny56posuffadh5a367n5vi4uq 356 7 target target NN work_sny56posuffadh5a367n5vi4uq 356 8 site site NN work_sny56posuffadh5a367n5vi4uq 356 9 selection selection NN work_sny56posuffadh5a367n5vi4uq 356 10 of of IN work_sny56posuffadh5a367n5vi4uq 356 11 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 356 12 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 356 13 and and CC work_sny56posuffadh5a367n5vi4uq 356 14 other other JJ work_sny56posuffadh5a367n5vi4uq 356 15 Tc1 tc1 CD work_sny56posuffadh5a367n5vi4uq 356 16 / / SYM work_sny56posuffadh5a367n5vi4uq 356 17 mariner mariner NN work_sny56posuffadh5a367n5vi4uq 356 18 transposable transposable JJ work_sny56posuffadh5a367n5vi4uq 356 19 elements element NNS work_sny56posuffadh5a367n5vi4uq 356 20 . . . work_sny56posuffadh5a367n5vi4uq 357 1 J J NNP work_sny56posuffadh5a367n5vi4uq 357 2 Mol Mol NNP work_sny56posuffadh5a367n5vi4uq 357 3 Biol Biol NNP work_sny56posuffadh5a367n5vi4uq 357 4 2002 2002 CD work_sny56posuffadh5a367n5vi4uq 357 5 ; ; : work_sny56posuffadh5a367n5vi4uq 357 6 323 323 CD work_sny56posuffadh5a367n5vi4uq 357 7 : : : work_sny56posuffadh5a367n5vi4uq 357 8 441 441 CD work_sny56posuffadh5a367n5vi4uq 357 9 – – : work_sny56posuffadh5a367n5vi4uq 357 10 452 452 CD work_sny56posuffadh5a367n5vi4uq 357 11 . . . work_sny56posuffadh5a367n5vi4uq 358 1 16 16 CD work_sny56posuffadh5a367n5vi4uq 358 2 . . . work_sny56posuffadh5a367n5vi4uq 359 1 Cui Cui NNP work_sny56posuffadh5a367n5vi4uq 359 2 Z Z NNP work_sny56posuffadh5a367n5vi4uq 359 3 , , , work_sny56posuffadh5a367n5vi4uq 359 4 Geurts Geurts NNP work_sny56posuffadh5a367n5vi4uq 359 5 AM am NN work_sny56posuffadh5a367n5vi4uq 359 6 , , , work_sny56posuffadh5a367n5vi4uq 359 7 Liu Liu NNP work_sny56posuffadh5a367n5vi4uq 359 8 G G NNP work_sny56posuffadh5a367n5vi4uq 359 9 , , , work_sny56posuffadh5a367n5vi4uq 359 10 et et NNP work_sny56posuffadh5a367n5vi4uq 359 11 al al NNP work_sny56posuffadh5a367n5vi4uq 359 12 . . . work_sny56posuffadh5a367n5vi4uq 360 1 Structure structure NN work_sny56posuffadh5a367n5vi4uq 360 2 - - HYPH work_sny56posuffadh5a367n5vi4uq 360 3 function function NN work_sny56posuffadh5a367n5vi4uq 360 4 analysis analysis NN work_sny56posuffadh5a367n5vi4uq 360 5 of of IN work_sny56posuffadh5a367n5vi4uq 360 6 the the DT work_sny56posuffadh5a367n5vi4uq 360 7 inverted inverted JJ work_sny56posuffadh5a367n5vi4uq 360 8 terminal terminal JJ work_sny56posuffadh5a367n5vi4uq 360 9 repeats repeat NNS work_sny56posuffadh5a367n5vi4uq 360 10 of of IN work_sny56posuffadh5a367n5vi4uq 360 11 the the DT work_sny56posuffadh5a367n5vi4uq 360 12 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 360 13 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 360 14 transposon transposon NNP work_sny56posuffadh5a367n5vi4uq 360 15 . . . work_sny56posuffadh5a367n5vi4uq 361 1 J J NNP work_sny56posuffadh5a367n5vi4uq 361 2 Mol Mol NNP work_sny56posuffadh5a367n5vi4uq 361 3 Biol Biol NNP work_sny56posuffadh5a367n5vi4uq 361 4 2002 2002 CD work_sny56posuffadh5a367n5vi4uq 361 5 ; ; : work_sny56posuffadh5a367n5vi4uq 361 6 318 318 CD work_sny56posuffadh5a367n5vi4uq 361 7 : : SYM work_sny56posuffadh5a367n5vi4uq 361 8 1221 1221 CD work_sny56posuffadh5a367n5vi4uq 361 9 – – : work_sny56posuffadh5a367n5vi4uq 361 10 1235 1235 CD work_sny56posuffadh5a367n5vi4uq 361 11 . . . work_sny56posuffadh5a367n5vi4uq 362 1 17 17 CD work_sny56posuffadh5a367n5vi4uq 362 2 . . . work_sny56posuffadh5a367n5vi4uq 363 1 Izsvak Izsvak NNP work_sny56posuffadh5a367n5vi4uq 363 2 Z Z NNP work_sny56posuffadh5a367n5vi4uq 363 3 , , , work_sny56posuffadh5a367n5vi4uq 363 4 Khare Khare NNP work_sny56posuffadh5a367n5vi4uq 363 5 D D NNP work_sny56posuffadh5a367n5vi4uq 363 6 , , , work_sny56posuffadh5a367n5vi4uq 363 7 Behlke Behlke NNP work_sny56posuffadh5a367n5vi4uq 363 8 J J NNP work_sny56posuffadh5a367n5vi4uq 363 9 , , , work_sny56posuffadh5a367n5vi4uq 363 10 et et NNP work_sny56posuffadh5a367n5vi4uq 363 11 al al NNP work_sny56posuffadh5a367n5vi4uq 363 12 . . . work_sny56posuffadh5a367n5vi4uq 364 1 Involvement involvement NN work_sny56posuffadh5a367n5vi4uq 364 2 of of IN work_sny56posuffadh5a367n5vi4uq 364 3 a a DT work_sny56posuffadh5a367n5vi4uq 364 4 bifunctional bifunctional JJ work_sny56posuffadh5a367n5vi4uq 364 5 , , , work_sny56posuffadh5a367n5vi4uq 364 6 paired pair VBN work_sny56posuffadh5a367n5vi4uq 364 7 - - HYPH work_sny56posuffadh5a367n5vi4uq 364 8 like like JJ work_sny56posuffadh5a367n5vi4uq 364 9 DNA dna NN work_sny56posuffadh5a367n5vi4uq 364 10 - - HYPH work_sny56posuffadh5a367n5vi4uq 364 11 binding bind VBG work_sny56posuffadh5a367n5vi4uq 364 12 domain domain NN work_sny56posuffadh5a367n5vi4uq 364 13 and and CC work_sny56posuffadh5a367n5vi4uq 364 14 a a DT work_sny56posuffadh5a367n5vi4uq 364 15 transpositional transpositional JJ work_sny56posuffadh5a367n5vi4uq 364 16 enhancer enhancer NN work_sny56posuffadh5a367n5vi4uq 364 17 in in IN work_sny56posuffadh5a367n5vi4uq 364 18 Sleeping Sleeping NNP work_sny56posuffadh5a367n5vi4uq 364 19 Beauty Beauty NNP work_sny56posuffadh5a367n5vi4uq 364 20 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 364 21 . . . work_sny56posuffadh5a367n5vi4uq 365 1 J J NNP work_sny56posuffadh5a367n5vi4uq 365 2 Biol Biol NNP work_sny56posuffadh5a367n5vi4uq 365 3 Chem Chem NNP work_sny56posuffadh5a367n5vi4uq 365 4 2002 2002 CD work_sny56posuffadh5a367n5vi4uq 365 5 ; ; : work_sny56posuffadh5a367n5vi4uq 365 6 277 277 CD work_sny56posuffadh5a367n5vi4uq 365 7 : : : work_sny56posuffadh5a367n5vi4uq 365 8 34 34 CD work_sny56posuffadh5a367n5vi4uq 365 9 581 581 CD work_sny56posuffadh5a367n5vi4uq 365 10 – – : work_sny56posuffadh5a367n5vi4uq 365 11 34 34 CD work_sny56posuffadh5a367n5vi4uq 365 12 588 588 CD work_sny56posuffadh5a367n5vi4uq 365 13 . . . work_sny56posuffadh5a367n5vi4uq 366 1 18 18 CD work_sny56posuffadh5a367n5vi4uq 366 2 . . . work_sny56posuffadh5a367n5vi4uq 367 1 Kawakami Kawakami NNP work_sny56posuffadh5a367n5vi4uq 367 2 K K NNP work_sny56posuffadh5a367n5vi4uq 367 3 , , , work_sny56posuffadh5a367n5vi4uq 367 4 Koga Koga NNP work_sny56posuffadh5a367n5vi4uq 367 5 A A NNP work_sny56posuffadh5a367n5vi4uq 367 6 , , , work_sny56posuffadh5a367n5vi4uq 367 7 Hori Hori NNP work_sny56posuffadh5a367n5vi4uq 367 8 H H NNP work_sny56posuffadh5a367n5vi4uq 367 9 , , , work_sny56posuffadh5a367n5vi4uq 367 10 Shima Shima NNP work_sny56posuffadh5a367n5vi4uq 367 11 A. a. NN work_sny56posuffadh5a367n5vi4uq 367 12 Excision Excision NNP work_sny56posuffadh5a367n5vi4uq 367 13 of of IN work_sny56posuffadh5a367n5vi4uq 367 14 the the DT work_sny56posuffadh5a367n5vi4uq 367 15 tol2 tol2 NN work_sny56posuffadh5a367n5vi4uq 367 16 transposable transposable JJ work_sny56posuffadh5a367n5vi4uq 367 17 element element NN work_sny56posuffadh5a367n5vi4uq 367 18 of of IN work_sny56posuffadh5a367n5vi4uq 367 19 the the DT work_sny56posuffadh5a367n5vi4uq 367 20 medaka medaka NN work_sny56posuffadh5a367n5vi4uq 367 21 fish fish NN work_sny56posuffadh5a367n5vi4uq 367 22 , , , work_sny56posuffadh5a367n5vi4uq 367 23 Oryzias Oryzias NNP work_sny56posuffadh5a367n5vi4uq 367 24 latipes latipe VBZ work_sny56posuffadh5a367n5vi4uq 367 25 , , , work_sny56posuffadh5a367n5vi4uq 367 26 in in IN work_sny56posuffadh5a367n5vi4uq 367 27 zebrafish zebrafish NNP work_sny56posuffadh5a367n5vi4uq 367 28 . . . work_sny56posuffadh5a367n5vi4uq 368 1 Danio Danio NNP work_sny56posuffadh5a367n5vi4uq 368 2 rerio rerio NN work_sny56posuffadh5a367n5vi4uq 368 3 . . . work_sny56posuffadh5a367n5vi4uq 369 1 Gene gene NN work_sny56posuffadh5a367n5vi4uq 369 2 1998 1998 CD work_sny56posuffadh5a367n5vi4uq 369 3 ; ; : work_sny56posuffadh5a367n5vi4uq 369 4 225 225 CD work_sny56posuffadh5a367n5vi4uq 369 5 : : SYM work_sny56posuffadh5a367n5vi4uq 369 6 17 17 CD work_sny56posuffadh5a367n5vi4uq 369 7 – – SYM work_sny56posuffadh5a367n5vi4uq 369 8 22 22 CD work_sny56posuffadh5a367n5vi4uq 369 9 . . . work_sny56posuffadh5a367n5vi4uq 370 1 19 19 CD work_sny56posuffadh5a367n5vi4uq 370 2 . . . work_sny56posuffadh5a367n5vi4uq 371 1 Kawakami Kawakami NNP work_sny56posuffadh5a367n5vi4uq 371 2 K K NNP work_sny56posuffadh5a367n5vi4uq 371 3 , , , work_sny56posuffadh5a367n5vi4uq 371 4 Shima Shima NNP work_sny56posuffadh5a367n5vi4uq 371 5 A. A. NNP work_sny56posuffadh5a367n5vi4uq 372 1 Identification identification NN work_sny56posuffadh5a367n5vi4uq 372 2 of of IN work_sny56posuffadh5a367n5vi4uq 372 3 the the DT work_sny56posuffadh5a367n5vi4uq 372 4 Tol2 tol2 JJ work_sny56posuffadh5a367n5vi4uq 372 5 transposase transposase NN work_sny56posuffadh5a367n5vi4uq 372 6 of of IN work_sny56posuffadh5a367n5vi4uq 372 7 the the DT work_sny56posuffadh5a367n5vi4uq 372 8 medaka medaka NN work_sny56posuffadh5a367n5vi4uq 372 9 fish fish NN work_sny56posuffadh5a367n5vi4uq 372 10 Oryzias Oryzias NNP work_sny56posuffadh5a367n5vi4uq 372 11 latipes latipe VBZ work_sny56posuffadh5a367n5vi4uq 372 12 that that WDT work_sny56posuffadh5a367n5vi4uq 372 13 catalyzes catalyze VBZ work_sny56posuffadh5a367n5vi4uq 372 14 excision excision NN work_sny56posuffadh5a367n5vi4uq 372 15 of of IN work_sny56posuffadh5a367n5vi4uq 372 16 a a DT work_sny56posuffadh5a367n5vi4uq 372 17 nonautonomous nonautonomous JJ work_sny56posuffadh5a367n5vi4uq 372 18 Tol2 Tol2 NNP work_sny56posuffadh5a367n5vi4uq 372 19 element element NN work_sny56posuffadh5a367n5vi4uq 372 20 in in IN work_sny56posuffadh5a367n5vi4uq 372 21 zebrafish zebrafish JJ work_sny56posuffadh5a367n5vi4uq 372 22 Danio Danio NNP work_sny56posuffadh5a367n5vi4uq 372 23 rerio rerio NN work_sny56posuffadh5a367n5vi4uq 372 24 . . . work_sny56posuffadh5a367n5vi4uq 373 1 Gene gene NN work_sny56posuffadh5a367n5vi4uq 373 2 1999 1999 CD work_sny56posuffadh5a367n5vi4uq 373 3 ; ; : work_sny56posuffadh5a367n5vi4uq 373 4 240 240 CD work_sny56posuffadh5a367n5vi4uq 373 5 : : : work_sny56posuffadh5a367n5vi4uq 373 6 239 239 CD work_sny56posuffadh5a367n5vi4uq 373 7 – – : work_sny56posuffadh5a367n5vi4uq 373 8 244 244 CD work_sny56posuffadh5a367n5vi4uq 373 9 . . . work_sny56posuffadh5a367n5vi4uq 374 1 20 20 CD work_sny56posuffadh5a367n5vi4uq 374 2 . . . work_sny56posuffadh5a367n5vi4uq 375 1 Available available JJ work_sny56posuffadh5a367n5vi4uq 375 2 : : : work_sny56posuffadh5a367n5vi4uq 375 3 http://www.cbs.umn.edu/labs/perry/. http://www.cbs.umn.edu/labs/perry/. NNP work_sny56posuffadh5a367n5vi4uq 376 1 21 21 CD work_sny56posuffadh5a367n5vi4uq 376 2 . . . work_sny56posuffadh5a367n5vi4uq 377 1 Liu Liu NNP work_sny56posuffadh5a367n5vi4uq 377 2 F F NNP work_sny56posuffadh5a367n5vi4uq 377 3 , , , work_sny56posuffadh5a367n5vi4uq 377 4 Song Song NNP work_sny56posuffadh5a367n5vi4uq 377 5 YK YK NNP work_sny56posuffadh5a367n5vi4uq 377 6 , , , work_sny56posuffadh5a367n5vi4uq 377 7 Liu Liu NNP work_sny56posuffadh5a367n5vi4uq 377 8 D. D. NNP work_sny56posuffadh5a367n5vi4uq 377 9 Hydrodynamics Hydrodynamics NNP work_sny56posuffadh5a367n5vi4uq 377 10 - - HYPH work_sny56posuffadh5a367n5vi4uq 377 11 based base VBN work_sny56posuffadh5a367n5vi4uq 377 12 transfection transfection NN work_sny56posuffadh5a367n5vi4uq 377 13 in in IN work_sny56posuffadh5a367n5vi4uq 377 14 animals animal NNS work_sny56posuffadh5a367n5vi4uq 377 15 by by IN work_sny56posuffadh5a367n5vi4uq 377 16 systemic systemic JJ work_sny56posuffadh5a367n5vi4uq 377 17 administration administration NNP work_sny56posuffadh5a367n5vi4uq 377 18 of of IN work_sny56posuffadh5a367n5vi4uq 377 19 plasmid plasmid NNP work_sny56posuffadh5a367n5vi4uq 377 20 DNA DNA NNP work_sny56posuffadh5a367n5vi4uq 377 21 . . . work_sny56posuffadh5a367n5vi4uq 378 1 Gene Gene NNP work_sny56posuffadh5a367n5vi4uq 378 2 Ther Ther NNP work_sny56posuffadh5a367n5vi4uq 378 3 1999 1999 CD work_sny56posuffadh5a367n5vi4uq 378 4 ; ; : work_sny56posuffadh5a367n5vi4uq 378 5 6 6 CD work_sny56posuffadh5a367n5vi4uq 378 6 : : SYM work_sny56posuffadh5a367n5vi4uq 378 7 1258 1258 CD work_sny56posuffadh5a367n5vi4uq 378 8 – – : work_sny56posuffadh5a367n5vi4uq 378 9 1266 1266 CD work_sny56posuffadh5a367n5vi4uq 378 10 . . . work_sny56posuffadh5a367n5vi4uq 379 1 22 22 CD work_sny56posuffadh5a367n5vi4uq 379 2 . . . work_sny56posuffadh5a367n5vi4uq 380 1 Zhang Zhang NNP work_sny56posuffadh5a367n5vi4uq 380 2 G G NNP work_sny56posuffadh5a367n5vi4uq 380 3 , , , work_sny56posuffadh5a367n5vi4uq 380 4 Budker Budker NNP work_sny56posuffadh5a367n5vi4uq 380 5 V V NNP work_sny56posuffadh5a367n5vi4uq 380 6 , , , work_sny56posuffadh5a367n5vi4uq 380 7 Wolff Wolff NNP work_sny56posuffadh5a367n5vi4uq 380 8 JA JA NNP work_sny56posuffadh5a367n5vi4uq 380 9 . . . work_sny56posuffadh5a367n5vi4uq 381 1 High high JJ work_sny56posuffadh5a367n5vi4uq 381 2 levels level NNS work_sny56posuffadh5a367n5vi4uq 381 3 of of IN work_sny56posuffadh5a367n5vi4uq 381 4 foreign foreign JJ work_sny56posuffadh5a367n5vi4uq 381 5 gene gene NN work_sny56posuffadh5a367n5vi4uq 381 6 expression expression NN work_sny56posuffadh5a367n5vi4uq 381 7 in in IN work_sny56posuffadh5a367n5vi4uq 381 8 hepatocytes hepatocyte NNS work_sny56posuffadh5a367n5vi4uq 381 9 after after IN work_sny56posuffadh5a367n5vi4uq 381 10 tail tail NN work_sny56posuffadh5a367n5vi4uq 381 11 vein vein NN work_sny56posuffadh5a367n5vi4uq 381 12 injections injection NNS work_sny56posuffadh5a367n5vi4uq 381 13 of of IN work_sny56posuffadh5a367n5vi4uq 381 14 naked naked JJ work_sny56posuffadh5a367n5vi4uq 381 15 plasmid plasmid NNP work_sny56posuffadh5a367n5vi4uq 381 16 DNA DNA NNP work_sny56posuffadh5a367n5vi4uq 381 17 . . . work_sny56posuffadh5a367n5vi4uq 382 1 Hum Hum NNP work_sny56posuffadh5a367n5vi4uq 382 2 Gene Gene NNP work_sny56posuffadh5a367n5vi4uq 382 3 Ther Ther NNP work_sny56posuffadh5a367n5vi4uq 382 4 1999 1999 CD work_sny56posuffadh5a367n5vi4uq 382 5 ; ; : work_sny56posuffadh5a367n5vi4uq 382 6 10 10 CD work_sny56posuffadh5a367n5vi4uq 382 7 : : SYM work_sny56posuffadh5a367n5vi4uq 382 8 1735 1735 CD work_sny56posuffadh5a367n5vi4uq 382 9 – – : work_sny56posuffadh5a367n5vi4uq 382 10 1737 1737 CD work_sny56posuffadh5a367n5vi4uq 382 11 . . . work_sny56posuffadh5a367n5vi4uq 383 1 23 23 CD work_sny56posuffadh5a367n5vi4uq 383 2 . . . work_sny56posuffadh5a367n5vi4uq 384 1 Birkenmeier Birkenmeier NNP work_sny56posuffadh5a367n5vi4uq 384 2 EH EH NNP work_sny56posuffadh5a367n5vi4uq 384 3 , , , work_sny56posuffadh5a367n5vi4uq 384 4 Davisson Davisson NNP work_sny56posuffadh5a367n5vi4uq 384 5 MT MT NNP work_sny56posuffadh5a367n5vi4uq 384 6 , , , work_sny56posuffadh5a367n5vi4uq 384 7 Bearner Bearner NNP work_sny56posuffadh5a367n5vi4uq 384 8 WG WG NNP work_sny56posuffadh5a367n5vi4uq 384 9 , , , work_sny56posuffadh5a367n5vi4uq 384 10 et et NNP work_sny56posuffadh5a367n5vi4uq 384 11 al al NNP work_sny56posuffadh5a367n5vi4uq 384 12 . . . work_sny56posuffadh5a367n5vi4uq 385 1 Murine Murine NNP work_sny56posuffadh5a367n5vi4uq 385 2 mucopolysaccharidosis mucopolysaccharidosis NN work_sny56posuffadh5a367n5vi4uq 385 3 VII vii NN work_sny56posuffadh5a367n5vi4uq 385 4 : : : work_sny56posuffadh5a367n5vi4uq 385 5 characterization characterization NN work_sny56posuffadh5a367n5vi4uq 385 6 of of IN work_sny56posuffadh5a367n5vi4uq 385 7 a a DT work_sny56posuffadh5a367n5vi4uq 385 8 mouse mouse NN work_sny56posuffadh5a367n5vi4uq 385 9 with with IN work_sny56posuffadh5a367n5vi4uq 385 10 β β NN work_sny56posuffadh5a367n5vi4uq 385 11 - - HYPH work_sny56posuffadh5a367n5vi4uq 385 12 glucuronidase glucuronidase NN work_sny56posuffadh5a367n5vi4uq 385 13 deficiency deficiency NN work_sny56posuffadh5a367n5vi4uq 385 14 . . . work_sny56posuffadh5a367n5vi4uq 386 1 J J NNP work_sny56posuffadh5a367n5vi4uq 386 2 Clin Clin NNP work_sny56posuffadh5a367n5vi4uq 386 3 Invest Invest NNP work_sny56posuffadh5a367n5vi4uq 386 4 1989 1989 CD work_sny56posuffadh5a367n5vi4uq 386 5 ; ; : work_sny56posuffadh5a367n5vi4uq 386 6 83 83 CD work_sny56posuffadh5a367n5vi4uq 386 7 : : SYM work_sny56posuffadh5a367n5vi4uq 386 8 1258 1258 CD work_sny56posuffadh5a367n5vi4uq 386 9 – – : work_sny56posuffadh5a367n5vi4uq 386 10 1266 1266 CD work_sny56posuffadh5a367n5vi4uq 386 11 . . . work_sny56posuffadh5a367n5vi4uq 387 1 24 24 CD work_sny56posuffadh5a367n5vi4uq 387 2 . . . work_sny56posuffadh5a367n5vi4uq 388 1 Critchlow Critchlow NNP work_sny56posuffadh5a367n5vi4uq 388 2 SE SE NNP work_sny56posuffadh5a367n5vi4uq 388 3 , , , work_sny56posuffadh5a367n5vi4uq 388 4 Jackson Jackson NNP work_sny56posuffadh5a367n5vi4uq 388 5 SP SP NNP work_sny56posuffadh5a367n5vi4uq 388 6 . . . work_sny56posuffadh5a367n5vi4uq 389 1 DNA dna NN work_sny56posuffadh5a367n5vi4uq 389 2 end end NN work_sny56posuffadh5a367n5vi4uq 389 3 - - HYPH work_sny56posuffadh5a367n5vi4uq 389 4 joining joining NN work_sny56posuffadh5a367n5vi4uq 389 5 : : : work_sny56posuffadh5a367n5vi4uq 389 6 from from IN work_sny56posuffadh5a367n5vi4uq 389 7 yeast yeast NN work_sny56posuffadh5a367n5vi4uq 389 8 to to IN work_sny56posuffadh5a367n5vi4uq 389 9 man man NN work_sny56posuffadh5a367n5vi4uq 389 10 . . . work_sny56posuffadh5a367n5vi4uq 390 1 Trends Trends NNPS work_sny56posuffadh5a367n5vi4uq 390 2 Biochem Biochem NNP work_sny56posuffadh5a367n5vi4uq 390 3 Sci Sci NNP work_sny56posuffadh5a367n5vi4uq 390 4 1998 1998 CD work_sny56posuffadh5a367n5vi4uq 390 5 ; ; : work_sny56posuffadh5a367n5vi4uq 390 6 23 23 CD work_sny56posuffadh5a367n5vi4uq 390 7 : : SYM work_sny56posuffadh5a367n5vi4uq 390 8 394 394 CD work_sny56posuffadh5a367n5vi4uq 390 9 – – : work_sny56posuffadh5a367n5vi4uq 390 10 398 398 CD work_sny56posuffadh5a367n5vi4uq 390 11 . . . work_sny56posuffadh5a367n5vi4uq 391 1 25 25 CD work_sny56posuffadh5a367n5vi4uq 391 2 . . . work_sny56posuffadh5a367n5vi4uq 392 1 Allingham Allingham NNP work_sny56posuffadh5a367n5vi4uq 392 2 JS JS NNP work_sny56posuffadh5a367n5vi4uq 392 3 , , , work_sny56posuffadh5a367n5vi4uq 392 4 Wardle Wardle NNP work_sny56posuffadh5a367n5vi4uq 392 5 SJ SJ NNP work_sny56posuffadh5a367n5vi4uq 392 6 , , , work_sny56posuffadh5a367n5vi4uq 392 7 Haniford Haniford NNP work_sny56posuffadh5a367n5vi4uq 392 8 DB DB NNP work_sny56posuffadh5a367n5vi4uq 392 9 . . . work_sny56posuffadh5a367n5vi4uq 393 1 Determinants determinant NNS work_sny56posuffadh5a367n5vi4uq 393 2 for for IN work_sny56posuffadh5a367n5vi4uq 393 3 hairpin hairpin NN work_sny56posuffadh5a367n5vi4uq 393 4 formation formation NN work_sny56posuffadh5a367n5vi4uq 393 5 in in IN work_sny56posuffadh5a367n5vi4uq 393 6 Tn10 Tn10 NNP work_sny56posuffadh5a367n5vi4uq 393 7 transposition transposition NN work_sny56posuffadh5a367n5vi4uq 393 8 . . . work_sny56posuffadh5a367n5vi4uq 394 1 EMBO EMBO NNP work_sny56posuffadh5a367n5vi4uq 394 2 J J NNP work_sny56posuffadh5a367n5vi4uq 394 3 2001 2001 CD work_sny56posuffadh5a367n5vi4uq 394 4 ; ; : work_sny56posuffadh5a367n5vi4uq 394 5 20 20 CD work_sny56posuffadh5a367n5vi4uq 394 6 : : SYM work_sny56posuffadh5a367n5vi4uq 394 7 2931 2931 CD work_sny56posuffadh5a367n5vi4uq 394 8 – – : work_sny56posuffadh5a367n5vi4uq 394 9 2942 2942 CD work_sny56posuffadh5a367n5vi4uq 394 10 . . . work_sny56posuffadh5a367n5vi4uq 395 1 26 26 CD work_sny56posuffadh5a367n5vi4uq 395 2 . . . work_sny56posuffadh5a367n5vi4uq 396 1 Coros Coros NNP work_sny56posuffadh5a367n5vi4uq 396 2 CJ CJ NNP work_sny56posuffadh5a367n5vi4uq 396 3 , , , work_sny56posuffadh5a367n5vi4uq 396 4 Chaconas Chaconas NNP work_sny56posuffadh5a367n5vi4uq 396 5 G. G. NNP work_sny56posuffadh5a367n5vi4uq 396 6 Effect Effect NNP work_sny56posuffadh5a367n5vi4uq 396 7 of of IN work_sny56posuffadh5a367n5vi4uq 396 8 mutations mutation NNS work_sny56posuffadh5a367n5vi4uq 396 9 in in IN work_sny56posuffadh5a367n5vi4uq 396 10 the the DT work_sny56posuffadh5a367n5vi4uq 396 11 Mu Mu NNP work_sny56posuffadh5a367n5vi4uq 396 12 - - HYPH work_sny56posuffadh5a367n5vi4uq 396 13 host host NN work_sny56posuffadh5a367n5vi4uq 396 14 junction junction NN work_sny56posuffadh5a367n5vi4uq 396 15 region region NN work_sny56posuffadh5a367n5vi4uq 396 16 on on IN work_sny56posuffadh5a367n5vi4uq 396 17 transpososome transpososome NN work_sny56posuffadh5a367n5vi4uq 396 18 assembly assembly NN work_sny56posuffadh5a367n5vi4uq 396 19 . . . work_sny56posuffadh5a367n5vi4uq 397 1 J J NNP work_sny56posuffadh5a367n5vi4uq 397 2 Mol Mol NNP work_sny56posuffadh5a367n5vi4uq 397 3 Biol Biol NNP work_sny56posuffadh5a367n5vi4uq 397 4 2001 2001 CD work_sny56posuffadh5a367n5vi4uq 397 5 ; ; : work_sny56posuffadh5a367n5vi4uq 397 6 310 310 CD work_sny56posuffadh5a367n5vi4uq 397 7 : : SYM work_sny56posuffadh5a367n5vi4uq 397 8 299 299 CD work_sny56posuffadh5a367n5vi4uq 397 9 – – : work_sny56posuffadh5a367n5vi4uq 397 10 309 309 CD work_sny56posuffadh5a367n5vi4uq 397 11 . . . work_sny56posuffadh5a367n5vi4uq 398 1 27 27 CD work_sny56posuffadh5a367n5vi4uq 398 2 . . . work_sny56posuffadh5a367n5vi4uq 399 1 Lee Lee NNP work_sny56posuffadh5a367n5vi4uq 399 2 I I NNP work_sny56posuffadh5a367n5vi4uq 399 3 , , , work_sny56posuffadh5a367n5vi4uq 399 4 Harshey Harshey NNP work_sny56posuffadh5a367n5vi4uq 399 5 RM RM NNP work_sny56posuffadh5a367n5vi4uq 399 6 . . . work_sny56posuffadh5a367n5vi4uq 400 1 Importance importance NN work_sny56posuffadh5a367n5vi4uq 400 2 of of IN work_sny56posuffadh5a367n5vi4uq 400 3 the the DT work_sny56posuffadh5a367n5vi4uq 400 4 conserved conserve VBN work_sny56posuffadh5a367n5vi4uq 400 5 CA CA NNP work_sny56posuffadh5a367n5vi4uq 400 6 dinucleotide dinucleotide VB work_sny56posuffadh5a367n5vi4uq 400 7 at at IN work_sny56posuffadh5a367n5vi4uq 400 8 Mu Mu NNP work_sny56posuffadh5a367n5vi4uq 400 9 termini termini NN work_sny56posuffadh5a367n5vi4uq 400 10 . . . work_sny56posuffadh5a367n5vi4uq 401 1 J J NNP work_sny56posuffadh5a367n5vi4uq 401 2 Mol Mol NNP work_sny56posuffadh5a367n5vi4uq 401 3 Biol Biol NNP work_sny56posuffadh5a367n5vi4uq 401 4 2001 2001 CD work_sny56posuffadh5a367n5vi4uq 401 5 ; ; : work_sny56posuffadh5a367n5vi4uq 401 6 314 314 CD work_sny56posuffadh5a367n5vi4uq 401 7 : : : work_sny56posuffadh5a367n5vi4uq 401 8 433 433 CD work_sny56posuffadh5a367n5vi4uq 401 9 – – : work_sny56posuffadh5a367n5vi4uq 401 10 444 444 CD work_sny56posuffadh5a367n5vi4uq 401 11 . . . work_sny56posuffadh5a367n5vi4uq 402 1 28 28 CD work_sny56posuffadh5a367n5vi4uq 402 2 . . . work_sny56posuffadh5a367n5vi4uq 403 1 Bhasin Bhasin NNP work_sny56posuffadh5a367n5vi4uq 403 2 A A NNP work_sny56posuffadh5a367n5vi4uq 403 3 , , , work_sny56posuffadh5a367n5vi4uq 403 4 Goryshin Goryshin NNP work_sny56posuffadh5a367n5vi4uq 403 5 IY IY NNP work_sny56posuffadh5a367n5vi4uq 403 6 , , , work_sny56posuffadh5a367n5vi4uq 403 7 Steiniger Steiniger NNP work_sny56posuffadh5a367n5vi4uq 403 8 - - HYPH work_sny56posuffadh5a367n5vi4uq 403 9 White White NNP work_sny56posuffadh5a367n5vi4uq 403 10 M M NNP work_sny56posuffadh5a367n5vi4uq 403 11 , , , work_sny56posuffadh5a367n5vi4uq 403 12 et et NNP work_sny56posuffadh5a367n5vi4uq 403 13 al al NNP work_sny56posuffadh5a367n5vi4uq 403 14 . . . work_sny56posuffadh5a367n5vi4uq 404 1 Characteriza- Characteriza- NNP work_sny56posuffadh5a367n5vi4uq 404 2 tion tion NN work_sny56posuffadh5a367n5vi4uq 404 3 of of IN work_sny56posuffadh5a367n5vi4uq 404 4 a a DT work_sny56posuffadh5a367n5vi4uq 404 5 Tn5 tn5 NN work_sny56posuffadh5a367n5vi4uq 404 6 pre pre JJ work_sny56posuffadh5a367n5vi4uq 404 7 - - JJ work_sny56posuffadh5a367n5vi4uq 404 8 cleavage cleavage JJ work_sny56posuffadh5a367n5vi4uq 404 9 synaptic synaptic JJ work_sny56posuffadh5a367n5vi4uq 404 10 complex complex NN work_sny56posuffadh5a367n5vi4uq 404 11 . . . work_sny56posuffadh5a367n5vi4uq 405 1 J J NNP work_sny56posuffadh5a367n5vi4uq 405 2 Mol Mol NNP work_sny56posuffadh5a367n5vi4uq 405 3 Biol Biol NNP work_sny56posuffadh5a367n5vi4uq 405 4 2000 2000 CD work_sny56posuffadh5a367n5vi4uq 405 5 ; ; : work_sny56posuffadh5a367n5vi4uq 405 6 302 302 CD work_sny56posuffadh5a367n5vi4uq 405 7 : : : work_sny56posuffadh5a367n5vi4uq 405 8 49 49 CD work_sny56posuffadh5a367n5vi4uq 405 9 – – SYM work_sny56posuffadh5a367n5vi4uq 405 10 63 63 CD work_sny56posuffadh5a367n5vi4uq 405 11 . . . work_sny56posuffadh5a367n5vi4uq 406 1 Copyright copyright NN work_sny56posuffadh5a367n5vi4uq 406 2   CD work_sny56posuffadh5a367n5vi4uq 406 3 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 406 4 John John NNP work_sny56posuffadh5a367n5vi4uq 406 5 Wiley Wiley NNP work_sny56posuffadh5a367n5vi4uq 406 6 & & CC work_sny56posuffadh5a367n5vi4uq 406 7 Sons Sons NNPS work_sny56posuffadh5a367n5vi4uq 406 8 , , , work_sny56posuffadh5a367n5vi4uq 406 9 Ltd. Ltd. NNP work_sny56posuffadh5a367n5vi4uq 406 10 J J NNP work_sny56posuffadh5a367n5vi4uq 406 11 Gene Gene NNP work_sny56posuffadh5a367n5vi4uq 406 12 Med Med NNP work_sny56posuffadh5a367n5vi4uq 406 13 2004 2004 CD work_sny56posuffadh5a367n5vi4uq 406 14 ; ; : work_sny56posuffadh5a367n5vi4uq 406 15 6 6 CD work_sny56posuffadh5a367n5vi4uq 406 16 : : : work_sny56posuffadh5a367n5vi4uq 406 17 574 574 CD work_sny56posuffadh5a367n5vi4uq 406 18 – – : work_sny56posuffadh5a367n5vi4uq 406 19 583 583 CD work_sny56posuffadh5a367n5vi4uq 406 20 . . .