Transcriptionally regulated and nontoxic delivery of the hyperactive Sleeping Beauty Transposase | Semantic Scholar Skip to search formSkip to main content> Semantic Scholar's Logo Search Sign InCreate Free Account You are currently offline. Some features of the site may not work correctly. DOI:10.1038/mtm.2016.38 Corpus ID: 23940126Transcriptionally regulated and nontoxic delivery of the hyperactive Sleeping Beauty Transposase @article{Cocchiarella2016TranscriptionallyRA, title={Transcriptionally regulated and nontoxic delivery of the hyperactive Sleeping Beauty Transposase}, author={Fabienne Cocchiarella and M. Latella and V. Basile and F. Miselli and M. Galla and Carol Imbriano and A. Recchia}, journal={Molecular Therapy. Methods & Clinical Development}, year={2016}, volume={3} } Fabienne Cocchiarella, M. Latella, +4 authors A. Recchia Published 2016 Biology, Medicine Molecular Therapy. Methods & Clinical Development The Sleeping Beauty (SB) transposase and, in particular, its hyperactive variant SB100X raises increasing interest for gene therapy application, including genome modification and, more recently, induced pluripotent stem cells (iPS) reprogramming. The documented cytotoxicity of the transposase, when constitutively expressed by an integrating retroviral vector (iRV), has been circumvented by the transient delivery of SB100X using retroviral mRNA transfer. In this study, we developed an… Expand View on PubMed cell.com Save to Library Create Alert Cite Launch Research Feed Share This Paper 2 CitationsBackground Citations 1 View All Figures and Topics from this paper figure 1 figure 2 figure 3 figure 4 figure 5 View All 5 Figures & Tables Hyperactive behavior Transposase Tetracycline Adverse reaction to drug Induced Pluripotent Stem Cells Retroviridae Trans-Activators keratinocyte Green Fluorescent Proteins Reprogram 2 Citations Citation Type Citation Type All Types Cites Results Cites Methods Cites Background Has PDF Publication Type Author More Filters More Filters Filters Sort by Relevance Sort by Most Influenced Papers Sort by Citation Count Sort by Recency Going non-viral: the Sleeping Beauty transposon system breaks on through to the clinical side M. Hudecek, Z. Izsvák, S. Johnen, M. Renner, G. Thumann, Z. 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