id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
10_1101-2020_12_31_424931	Banchenko, Sofia	Structural insights into Cullin4-RING ubiquitin ligase remodelling by Vpr from simian immunodeficiency viruses	2021	45	.pdf	application/pdf	16409	1983	68	SDS-PAGE shows that SAMHD1-ΔCtD did not co-elute with DDB1/DCAF1-CtD/Vprmus (Fig 1A, B, 138 Crystal Structure analysis of apoand Vprmus-bound DDB1/DCAF1-CtD protein 154 Superposition of the apo-DDB1/DCAF1-CtD and Vprmus-bound crystal structures reveals 176 DDB1/DCAF1-CtD/Vprmus/SAMHD1 complex peak (Fig 2D, fraction 6), when compared to the wild 199 To obtain mechanistic insight into Vprmus-recruitment of SAMHD1-CtD, we initiated cryo-EM analyses 209 Molecular models of DDB1 BP domains A and C (BPA, BPC), DCAF1-CtD and Vprmus, derived from 218 biochemical data, showing that SAMHD1-CtD is sufficient for recruitment to DDB1/DCAF1/Vprmus, 226 SAMHD1-CtD cross-links were with the C-terminus of CUL4 and the "acidic loop" of DCAF1 (Fig 245 A66W mutant showed a reduction of DDB1/DCAF1-CtD/Vprmus/SAMHD1 complex peak intensity (Fig 261 Like SIV Vpx, "hybrid" Vpr proteins down-regulate the host restriction factor SAMHD1 by recruiting 324 both DDB1/DCAF1-CtD/Vprmus (core) and CUL4/ROC1 (stalk). (A) GF analysis of in vitro reconstitution of protein complexes containing DDB1/DCAF1-CtD, Vprmus 972 Crystal structure of the DDB1/DCAF1-CtD/Vprmus complex.	./cache/10_1101-2020_12_31_424931.pdf	./txt/10_1101-2020_12_31_424931.txt