id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
10_1101-2021_01_07_425675	Peng, Weiwei	Mass spectrometry-based sequencing of the anti-FLAG-M2 antibody using multiple proteases and a dual fragmentation scheme	2021	17	.pdf	application/pdf	7264	569	60	Mass spectrometry-based sequencing of the anti-FLAG-M2 antibody using multiple proteases and a dual fragmentation scheme Mass spectrometry-based sequencing of the anti-FLAG-M2 antibody using multiple 1 mass spectrometry, antibody, de novo sequencing, EThcD, stepped HCD, Herceptin, FLAG tag, 16 method for direct de novo sequencing of monoclonal IgG from the purified antibody products. Direct mass spectrometry (MS)-based sequencing of the secreted antibody products is a useful 50 protocol achieved full sequence coverage of the variable domains of both heavy and light chains, 81 mass spectrometry-based de novo sequencing of the monoclonal antibody Herceptin. Mass spectrometry based de novo sequence of the mouse monoclonal anti-FLAG-M2 antibody. We next applied our sequencing protocol to the mouse monoclonal anti-FLAG-M2 antibody as a 159 antibody produced with the experimentally determined sequence demonstrate equivalent FLAG-tag binding 181 There are four other monoclonal antibody sequences against the FLAG tag publicly available 205 	./cache/10_1101-2021_01_07_425675.pdf	./txt/10_1101-2021_01_07_425675.txt