id sid tid token lemma pos 10_1101-2020_11_24_390039 1 1 O'Keefe O'Keefe NNP 10_1101-2020_11_24_390039 1 2 et et NNP 10_1101-2020_11_24_390039 1 3 al al NNP 10_1101-2020_11_24_390039 1 4 . . . 10_1101-2020_11_24_390039 2 1 JOCES JOCES NNP 10_1101-2020_11_24_390039 2 2 2020 2020 CD 10_1101-2020_11_24_390039 2 3 257758 257758 CD 10_1101-2020_11_24_390039 2 4 resubmission resubmission NN 10_1101-2020_11_24_390039 2 5 Ipomoeassin Ipomoeassin NNP 10_1101-2020_11_24_390039 2 6 - - HYPH 10_1101-2020_11_24_390039 2 7 F F NNP 10_1101-2020_11_24_390039 2 8 inhibits inhibit VBZ 10_1101-2020_11_24_390039 2 9 the the DT 10_1101-2020_11_24_390039 2 10 in in FW 10_1101-2020_11_24_390039 2 11 vitro vitro FW 10_1101-2020_11_24_390039 2 12 biogenesis biogenesis NN 10_1101-2020_11_24_390039 2 13 of of IN 10_1101-2020_11_24_390039 2 14 the the DT 10_1101-2020_11_24_390039 2 15 SARS- SARS- NNP 10_1101-2020_11_24_390039 2 16 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 2 17 spike spike JJ 10_1101-2020_11_24_390039 2 18 protein protein NN 10_1101-2020_11_24_390039 2 19 and and CC 10_1101-2020_11_24_390039 2 20 its -PRON- PRP$ 10_1101-2020_11_24_390039 2 21 host host NN 10_1101-2020_11_24_390039 2 22 cell cell NN 10_1101-2020_11_24_390039 2 23 membrane membrane NN 10_1101-2020_11_24_390039 2 24 receptor receptor NN 10_1101-2020_11_24_390039 2 25 Sarah Sarah NNP 10_1101-2020_11_24_390039 2 26 O’Keefe1,4 O’Keefe1,4 NFP 10_1101-2020_11_24_390039 2 27 , , , 10_1101-2020_11_24_390039 2 28 Peristera Peristera NNP 10_1101-2020_11_24_390039 2 29 Roboti1 Roboti1 NNP 10_1101-2020_11_24_390039 2 30 , , , 10_1101-2020_11_24_390039 2 31 Kwabena Kwabena NNP 10_1101-2020_11_24_390039 2 32 B. B. NNP 10_1101-2020_11_24_390039 2 33 Duah2 Duah2 VBZ 10_1101-2020_11_24_390039 2 34 , , , 10_1101-2020_11_24_390039 2 35 Guanghui Guanghui NNP 10_1101-2020_11_24_390039 2 36 Zong3 zong3 NN 10_1101-2020_11_24_390039 2 37 , , , 10_1101-2020_11_24_390039 2 38 Hayden Hayden NNP 10_1101-2020_11_24_390039 2 39 Schneider2 Schneider2 NNP 10_1101-2020_11_24_390039 2 40 , , , 10_1101-2020_11_24_390039 2 41 Wei Wei NNP 10_1101-2020_11_24_390039 2 42 Q. Q. NNP 10_1101-2020_11_24_390039 2 43 Shi2 Shi2 NNP 10_1101-2020_11_24_390039 2 44 and and CC 10_1101-2020_11_24_390039 2 45 Stephen Stephen NNP 10_1101-2020_11_24_390039 2 46 High1,4 High1,4 NNP 10_1101-2020_11_24_390039 2 47 1School 1school CD 10_1101-2020_11_24_390039 2 48 of of IN 10_1101-2020_11_24_390039 2 49 Biological Biological NNP 10_1101-2020_11_24_390039 2 50 Sciences Sciences NNPS 10_1101-2020_11_24_390039 2 51 , , , 10_1101-2020_11_24_390039 2 52 Faculty Faculty NNP 10_1101-2020_11_24_390039 2 53 of of IN 10_1101-2020_11_24_390039 2 54 Biology Biology NNP 10_1101-2020_11_24_390039 2 55 , , , 10_1101-2020_11_24_390039 2 56 Medicine Medicine NNP 10_1101-2020_11_24_390039 2 57 and and CC 10_1101-2020_11_24_390039 2 58 Health Health NNP 10_1101-2020_11_24_390039 2 59 , , , 10_1101-2020_11_24_390039 2 60 University University NNP 10_1101-2020_11_24_390039 2 61 of of IN 10_1101-2020_11_24_390039 2 62 Manchester Manchester NNP 10_1101-2020_11_24_390039 2 63 , , , 10_1101-2020_11_24_390039 2 64 Manchester Manchester NNP 10_1101-2020_11_24_390039 2 65 , , , 10_1101-2020_11_24_390039 2 66 M13 M13 NNP 10_1101-2020_11_24_390039 2 67 9PT 9PT NNP 10_1101-2020_11_24_390039 2 68 , , , 10_1101-2020_11_24_390039 2 69 United United NNP 10_1101-2020_11_24_390039 2 70 Kingdom Kingdom NNP 10_1101-2020_11_24_390039 2 71 2Department 2department CD 10_1101-2020_11_24_390039 2 72 of of IN 10_1101-2020_11_24_390039 2 73 Chemistry Chemistry NNP 10_1101-2020_11_24_390039 2 74 , , , 10_1101-2020_11_24_390039 2 75 Ball Ball NNP 10_1101-2020_11_24_390039 2 76 State State NNP 10_1101-2020_11_24_390039 2 77 University University NNP 10_1101-2020_11_24_390039 2 78 , , , 10_1101-2020_11_24_390039 2 79 Muncie Muncie NNP 10_1101-2020_11_24_390039 2 80 , , , 10_1101-2020_11_24_390039 2 81 Indiana Indiana NNP 10_1101-2020_11_24_390039 2 82 47306 47306 CD 10_1101-2020_11_24_390039 2 83 , , , 10_1101-2020_11_24_390039 2 84 USA USA NNP 10_1101-2020_11_24_390039 2 85 3Department 3Department NNP 10_1101-2020_11_24_390039 2 86 of of IN 10_1101-2020_11_24_390039 2 87 Chemistry Chemistry NNP 10_1101-2020_11_24_390039 2 88 and and CC 10_1101-2020_11_24_390039 2 89 Biochemistry Biochemistry NNP 10_1101-2020_11_24_390039 2 90 , , , 10_1101-2020_11_24_390039 2 91 University University NNP 10_1101-2020_11_24_390039 2 92 of of IN 10_1101-2020_11_24_390039 2 93 Maryland Maryland NNP 10_1101-2020_11_24_390039 2 94 , , , 10_1101-2020_11_24_390039 2 95 College College NNP 10_1101-2020_11_24_390039 2 96 Park Park NNP 10_1101-2020_11_24_390039 2 97 , , , 10_1101-2020_11_24_390039 2 98 Maryland Maryland NNP 10_1101-2020_11_24_390039 2 99 20742 20742 CD 10_1101-2020_11_24_390039 2 100 , , , 10_1101-2020_11_24_390039 2 101 USA USA NNP 10_1101-2020_11_24_390039 2 102 4Lead 4lead CD 10_1101-2020_11_24_390039 2 103 Contacts Contacts NNPS 10_1101-2020_11_24_390039 2 104 for for IN 10_1101-2020_11_24_390039 2 105 correspondence correspondence NN 10_1101-2020_11_24_390039 2 106 : : : 10_1101-2020_11_24_390039 2 107 sarah.okeefe@manchester.ac.uk sarah.okeefe@manchester.ac.uk NNS 10_1101-2020_11_24_390039 2 108 ; ; : 10_1101-2020_11_24_390039 2 109 stephen.high@manchester.ac.uk stephen.high@manchester.ac.uk NNS 10_1101-2020_11_24_390039 2 110 Running Running NNP 10_1101-2020_11_24_390039 2 111 Title Title NNP 10_1101-2020_11_24_390039 2 112 Ipom Ipom NNP 10_1101-2020_11_24_390039 2 113 - - HYPH 10_1101-2020_11_24_390039 2 114 F F NNP 10_1101-2020_11_24_390039 2 115 as as IN 10_1101-2020_11_24_390039 2 116 a a DT 10_1101-2020_11_24_390039 2 117 potential potential JJ 10_1101-2020_11_24_390039 2 118 antiviral antiviral JJ 10_1101-2020_11_24_390039 2 119 agent agent NN 10_1101-2020_11_24_390039 2 120 Keywords Keywords NNPS 10_1101-2020_11_24_390039 2 121 Cell Cell NNP 10_1101-2020_11_24_390039 2 122 - - HYPH 10_1101-2020_11_24_390039 2 123 free free JJ 10_1101-2020_11_24_390039 2 124 translation translation NN 10_1101-2020_11_24_390039 2 125 , , , 10_1101-2020_11_24_390039 2 126 Endoplasmic endoplasmic JJ 10_1101-2020_11_24_390039 2 127 reticulum reticulum NN 10_1101-2020_11_24_390039 2 128 ( ( -LRB- 10_1101-2020_11_24_390039 2 129 ER ER NNP 10_1101-2020_11_24_390039 2 130 ) ) -RRB- 10_1101-2020_11_24_390039 2 131 , , , 10_1101-2020_11_24_390039 2 132 ER ER NNP 10_1101-2020_11_24_390039 2 133 membrane membrane NN 10_1101-2020_11_24_390039 2 134 complex complex NN 10_1101-2020_11_24_390039 2 135 ( ( -LRB- 10_1101-2020_11_24_390039 2 136 EMC EMC NNP 10_1101-2020_11_24_390039 2 137 ) ) -RRB- 10_1101-2020_11_24_390039 2 138 , , , 10_1101-2020_11_24_390039 2 139 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 2 140 translocon translocon NN 10_1101-2020_11_24_390039 2 141 , , , 10_1101-2020_11_24_390039 2 142 viral viral JJ 10_1101-2020_11_24_390039 2 143 protein protein NN 10_1101-2020_11_24_390039 2 144 biogenesis biogenesis NN 10_1101-2020_11_24_390039 2 145 . . . 10_1101-2020_11_24_390039 3 1 .CC .CC NFP 10_1101-2020_11_24_390039 3 2 - - : 10_1101-2020_11_24_390039 3 3 BY by IN 10_1101-2020_11_24_390039 3 4 - - HYPH 10_1101-2020_11_24_390039 3 5 ND ND NNP 10_1101-2020_11_24_390039 3 6 4.0 4.0 CD 10_1101-2020_11_24_390039 3 7 International international JJ 10_1101-2020_11_24_390039 3 8 licensemade licensemade NN 10_1101-2020_11_24_390039 3 9 available available JJ 10_1101-2020_11_24_390039 3 10 under under IN 10_1101-2020_11_24_390039 3 11 a a DT 10_1101-2020_11_24_390039 3 12 ( ( -LRB- 10_1101-2020_11_24_390039 3 13 which which WDT 10_1101-2020_11_24_390039 3 14 was be VBD 10_1101-2020_11_24_390039 3 15 not not RB 10_1101-2020_11_24_390039 3 16 certified certify VBN 10_1101-2020_11_24_390039 3 17 by by IN 10_1101-2020_11_24_390039 3 18 peer peer NN 10_1101-2020_11_24_390039 3 19 review review NN 10_1101-2020_11_24_390039 3 20 ) ) -RRB- 10_1101-2020_11_24_390039 3 21 is be VBZ 10_1101-2020_11_24_390039 3 22 the the DT 10_1101-2020_11_24_390039 3 23 author author NN 10_1101-2020_11_24_390039 3 24 / / SYM 10_1101-2020_11_24_390039 3 25 funder funder NN 10_1101-2020_11_24_390039 3 26 , , , 10_1101-2020_11_24_390039 3 27 who who WP 10_1101-2020_11_24_390039 3 28 has have VBZ 10_1101-2020_11_24_390039 3 29 granted grant VBN 10_1101-2020_11_24_390039 3 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 3 31 a a DT 10_1101-2020_11_24_390039 3 32 license license NN 10_1101-2020_11_24_390039 3 33 to to TO 10_1101-2020_11_24_390039 3 34 display display VB 10_1101-2020_11_24_390039 3 35 the the DT 10_1101-2020_11_24_390039 3 36 preprint preprint NN 10_1101-2020_11_24_390039 3 37 in in IN 10_1101-2020_11_24_390039 3 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 3 39 . . . 10_1101-2020_11_24_390039 4 1 It -PRON- PRP 10_1101-2020_11_24_390039 4 2 is be VBZ 10_1101-2020_11_24_390039 4 3 The the DT 10_1101-2020_11_24_390039 4 4 copyright copyright NN 10_1101-2020_11_24_390039 4 5 holder holder NN 10_1101-2020_11_24_390039 4 6 for for IN 10_1101-2020_11_24_390039 4 7 this this DT 10_1101-2020_11_24_390039 4 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 4 9 version version NN 10_1101-2020_11_24_390039 4 10 posted post VBD 10_1101-2020_11_24_390039 4 11 January January NNP 10_1101-2020_11_24_390039 4 12 5 5 CD 10_1101-2020_11_24_390039 4 13 , , , 10_1101-2020_11_24_390039 4 14 2021 2021 CD 10_1101-2020_11_24_390039 4 15 . . . 10_1101-2020_11_24_390039 4 16 ; ; : 10_1101-2020_11_24_390039 4 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 4 18 : : : 10_1101-2020_11_24_390039 4 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 4 20 preprint preprint JJ 10_1101-2020_11_24_390039 4 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 4 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 4 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 4 24 - - HYPH 10_1101-2020_11_24_390039 4 25 F F NNP 10_1101-2020_11_24_390039 4 26 as as IN 10_1101-2020_11_24_390039 4 27 a a DT 10_1101-2020_11_24_390039 4 28 potential potential JJ 10_1101-2020_11_24_390039 4 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 4 30 agent agent NN 10_1101-2020_11_24_390039 4 31 Page Page NNP 10_1101-2020_11_24_390039 4 32 2 2 CD 10_1101-2020_11_24_390039 4 33 of of IN 10_1101-2020_11_24_390039 4 34 21 21 CD 10_1101-2020_11_24_390039 4 35 Abstract Abstract NNP 10_1101-2020_11_24_390039 4 36 In in IN 10_1101-2020_11_24_390039 4 37 order order NN 10_1101-2020_11_24_390039 4 38 to to TO 10_1101-2020_11_24_390039 4 39 produce produce VB 10_1101-2020_11_24_390039 4 40 proteins protein NNS 10_1101-2020_11_24_390039 4 41 essential essential JJ 10_1101-2020_11_24_390039 4 42 for for IN 10_1101-2020_11_24_390039 4 43 their -PRON- PRP$ 10_1101-2020_11_24_390039 4 44 propagation propagation NN 10_1101-2020_11_24_390039 4 45 , , , 10_1101-2020_11_24_390039 4 46 many many JJ 10_1101-2020_11_24_390039 4 47 pathogenic pathogenic JJ 10_1101-2020_11_24_390039 4 48 human human JJ 10_1101-2020_11_24_390039 4 49 viruses virus NNS 10_1101-2020_11_24_390039 4 50 , , , 10_1101-2020_11_24_390039 4 51 including include VBG 10_1101-2020_11_24_390039 4 52 SARS SARS NNP 10_1101-2020_11_24_390039 4 53 - - HYPH 10_1101-2020_11_24_390039 4 54 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 4 55 the the DT 10_1101-2020_11_24_390039 4 56 causative causative JJ 10_1101-2020_11_24_390039 4 57 agent agent NN 10_1101-2020_11_24_390039 4 58 of of IN 10_1101-2020_11_24_390039 4 59 COVID-19 covid-19 JJ 10_1101-2020_11_24_390039 4 60 respiratory respiratory JJ 10_1101-2020_11_24_390039 4 61 disease disease NN 10_1101-2020_11_24_390039 4 62 , , , 10_1101-2020_11_24_390039 4 63 commandeer commandeer NNP 10_1101-2020_11_24_390039 4 64 host host NN 10_1101-2020_11_24_390039 4 65 biosynthetic biosynthetic JJ 10_1101-2020_11_24_390039 4 66 machineries machinery NNS 10_1101-2020_11_24_390039 4 67 and and CC 10_1101-2020_11_24_390039 4 68 mechanisms mechanism NNS 10_1101-2020_11_24_390039 4 69 . . . 10_1101-2020_11_24_390039 5 1 Three three CD 10_1101-2020_11_24_390039 5 2 major major JJ 10_1101-2020_11_24_390039 5 3 structural structural JJ 10_1101-2020_11_24_390039 5 4 proteins protein NNS 10_1101-2020_11_24_390039 5 5 , , , 10_1101-2020_11_24_390039 5 6 the the DT 10_1101-2020_11_24_390039 5 7 spike spike NN 10_1101-2020_11_24_390039 5 8 , , , 10_1101-2020_11_24_390039 5 9 envelope envelope NN 10_1101-2020_11_24_390039 5 10 and and CC 10_1101-2020_11_24_390039 5 11 membrane membrane NN 10_1101-2020_11_24_390039 5 12 proteins protein NNS 10_1101-2020_11_24_390039 5 13 , , , 10_1101-2020_11_24_390039 5 14 are be VBP 10_1101-2020_11_24_390039 5 15 amongst amongst IN 10_1101-2020_11_24_390039 5 16 several several JJ 10_1101-2020_11_24_390039 5 17 SARS SARS NNP 10_1101-2020_11_24_390039 5 18 - - HYPH 10_1101-2020_11_24_390039 5 19 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 5 20 components component NNS 10_1101-2020_11_24_390039 5 21 synthesised synthesise VBD 10_1101-2020_11_24_390039 5 22 at at IN 10_1101-2020_11_24_390039 5 23 the the DT 10_1101-2020_11_24_390039 5 24 endoplasmic endoplasmic JJ 10_1101-2020_11_24_390039 5 25 reticulum reticulum NN 10_1101-2020_11_24_390039 5 26 ( ( -LRB- 10_1101-2020_11_24_390039 5 27 ER ER NNP 10_1101-2020_11_24_390039 5 28 ) ) -RRB- 10_1101-2020_11_24_390039 5 29 of of IN 10_1101-2020_11_24_390039 5 30 infected infect VBN 10_1101-2020_11_24_390039 5 31 human human JJ 10_1101-2020_11_24_390039 5 32 cells cell NNS 10_1101-2020_11_24_390039 5 33 prior prior RB 10_1101-2020_11_24_390039 5 34 to to IN 10_1101-2020_11_24_390039 5 35 the the DT 10_1101-2020_11_24_390039 5 36 assembly assembly NN 10_1101-2020_11_24_390039 5 37 of of IN 10_1101-2020_11_24_390039 5 38 new new JJ 10_1101-2020_11_24_390039 5 39 viral viral JJ 10_1101-2020_11_24_390039 5 40 particles particle NNS 10_1101-2020_11_24_390039 5 41 . . . 10_1101-2020_11_24_390039 6 1 Hence hence RB 10_1101-2020_11_24_390039 6 2 , , , 10_1101-2020_11_24_390039 6 3 the the DT 10_1101-2020_11_24_390039 6 4 inhibition inhibition NN 10_1101-2020_11_24_390039 6 5 of of IN 10_1101-2020_11_24_390039 6 6 membrane membrane NN 10_1101-2020_11_24_390039 6 7 protein protein NN 10_1101-2020_11_24_390039 6 8 synthesis synthesis NN 10_1101-2020_11_24_390039 6 9 at at IN 10_1101-2020_11_24_390039 6 10 the the DT 10_1101-2020_11_24_390039 6 11 ER ER NNP 10_1101-2020_11_24_390039 6 12 is be VBZ 10_1101-2020_11_24_390039 6 13 an an DT 10_1101-2020_11_24_390039 6 14 attractive attractive JJ 10_1101-2020_11_24_390039 6 15 strategy strategy NN 10_1101-2020_11_24_390039 6 16 for for IN 10_1101-2020_11_24_390039 6 17 reducing reduce VBG 10_1101-2020_11_24_390039 6 18 the the DT 10_1101-2020_11_24_390039 6 19 pathogenicity pathogenicity NN 10_1101-2020_11_24_390039 6 20 of of IN 10_1101-2020_11_24_390039 6 21 SARS SARS NNP 10_1101-2020_11_24_390039 6 22 - - HYPH 10_1101-2020_11_24_390039 6 23 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 6 24 and and CC 10_1101-2020_11_24_390039 6 25 other other JJ 10_1101-2020_11_24_390039 6 26 obligate obligate NN 10_1101-2020_11_24_390039 6 27 viral viral JJ 10_1101-2020_11_24_390039 6 28 pathogens pathogen NNS 10_1101-2020_11_24_390039 6 29 . . . 10_1101-2020_11_24_390039 7 1 Using use VBG 10_1101-2020_11_24_390039 7 2 an an DT 10_1101-2020_11_24_390039 7 3 in in FW 10_1101-2020_11_24_390039 7 4 vitro vitro FW 10_1101-2020_11_24_390039 7 5 system system NN 10_1101-2020_11_24_390039 7 6 , , , 10_1101-2020_11_24_390039 7 7 we -PRON- PRP 10_1101-2020_11_24_390039 7 8 demonstrate demonstrate VBP 10_1101-2020_11_24_390039 7 9 that that IN 10_1101-2020_11_24_390039 7 10 the the DT 10_1101-2020_11_24_390039 7 11 small small JJ 10_1101-2020_11_24_390039 7 12 molecule molecule NN 10_1101-2020_11_24_390039 7 13 inhibitor inhibitor NN 10_1101-2020_11_24_390039 7 14 ipomoeassin ipomoeassin NNP 10_1101-2020_11_24_390039 7 15 F F NNP 10_1101-2020_11_24_390039 7 16 ( ( -LRB- 10_1101-2020_11_24_390039 7 17 Ipom Ipom NNP 10_1101-2020_11_24_390039 7 18 - - HYPH 10_1101-2020_11_24_390039 7 19 F F NNP 10_1101-2020_11_24_390039 7 20 ) ) -RRB- 10_1101-2020_11_24_390039 7 21 potently potently RB 10_1101-2020_11_24_390039 7 22 blocks block VBZ 10_1101-2020_11_24_390039 7 23 the the DT 10_1101-2020_11_24_390039 7 24 Sec61-mediated sec61-mediated JJ 10_1101-2020_11_24_390039 7 25 ER ER NNP 10_1101-2020_11_24_390039 7 26 membrane membrane NN 10_1101-2020_11_24_390039 7 27 translocation translocation NN 10_1101-2020_11_24_390039 7 28 / / SYM 10_1101-2020_11_24_390039 7 29 insertion insertion NN 10_1101-2020_11_24_390039 7 30 of of IN 10_1101-2020_11_24_390039 7 31 three three CD 10_1101-2020_11_24_390039 7 32 therapeutic therapeutic JJ 10_1101-2020_11_24_390039 7 33 protein protein NN 10_1101-2020_11_24_390039 7 34 targets target NNS 10_1101-2020_11_24_390039 7 35 for for IN 10_1101-2020_11_24_390039 7 36 SARS SARS NNP 10_1101-2020_11_24_390039 7 37 - - HYPH 10_1101-2020_11_24_390039 7 38 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 7 39 infection infection NN 10_1101-2020_11_24_390039 7 40 ; ; : 10_1101-2020_11_24_390039 7 41 the the DT 10_1101-2020_11_24_390039 7 42 viral viral JJ 10_1101-2020_11_24_390039 7 43 spike spike NN 10_1101-2020_11_24_390039 7 44 and and CC 10_1101-2020_11_24_390039 7 45 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 7 46 proteins protein VBZ 10_1101-2020_11_24_390039 7 47 together together RB 10_1101-2020_11_24_390039 7 48 with with IN 10_1101-2020_11_24_390039 7 49 angiotensin angiotensin NN 10_1101-2020_11_24_390039 7 50 - - HYPH 10_1101-2020_11_24_390039 7 51 converting convert VBG 10_1101-2020_11_24_390039 7 52 enzyme enzyme NNS 10_1101-2020_11_24_390039 7 53 2 2 CD 10_1101-2020_11_24_390039 7 54 , , , 10_1101-2020_11_24_390039 7 55 the the DT 10_1101-2020_11_24_390039 7 56 host host NN 10_1101-2020_11_24_390039 7 57 cell cell NN 10_1101-2020_11_24_390039 7 58 plasma plasma NNP 10_1101-2020_11_24_390039 7 59 membrane membrane NN 10_1101-2020_11_24_390039 7 60 receptor receptor NN 10_1101-2020_11_24_390039 7 61 . . . 10_1101-2020_11_24_390039 8 1 Our -PRON- PRP$ 10_1101-2020_11_24_390039 8 2 findings finding NNS 10_1101-2020_11_24_390039 8 3 highlight highlight VBP 10_1101-2020_11_24_390039 8 4 the the DT 10_1101-2020_11_24_390039 8 5 potential potential NN 10_1101-2020_11_24_390039 8 6 for for IN 10_1101-2020_11_24_390039 8 7 using use VBG 10_1101-2020_11_24_390039 8 8 ER ER NNP 10_1101-2020_11_24_390039 8 9 protein protein NN 10_1101-2020_11_24_390039 8 10 translocation translocation NN 10_1101-2020_11_24_390039 8 11 inhibitors inhibitor NNS 10_1101-2020_11_24_390039 8 12 such such JJ 10_1101-2020_11_24_390039 8 13 as as IN 10_1101-2020_11_24_390039 8 14 Ipom Ipom NNP 10_1101-2020_11_24_390039 8 15 - - HYPH 10_1101-2020_11_24_390039 8 16 F F NNP 10_1101-2020_11_24_390039 8 17 as as IN 10_1101-2020_11_24_390039 8 18 host host NN 10_1101-2020_11_24_390039 8 19 - - HYPH 10_1101-2020_11_24_390039 8 20 targeting targeting JJ 10_1101-2020_11_24_390039 8 21 , , , 10_1101-2020_11_24_390039 8 22 broad broad JJ 10_1101-2020_11_24_390039 8 23 - - HYPH 10_1101-2020_11_24_390039 8 24 spectrum spectrum NN 10_1101-2020_11_24_390039 8 25 , , , 10_1101-2020_11_24_390039 8 26 antiviral antiviral JJ 10_1101-2020_11_24_390039 8 27 agents agent NNS 10_1101-2020_11_24_390039 8 28 . . . 10_1101-2020_11_24_390039 9 1 .CC .CC NFP 10_1101-2020_11_24_390039 9 2 - - : 10_1101-2020_11_24_390039 9 3 BY by IN 10_1101-2020_11_24_390039 9 4 - - HYPH 10_1101-2020_11_24_390039 9 5 ND ND NNP 10_1101-2020_11_24_390039 9 6 4.0 4.0 CD 10_1101-2020_11_24_390039 9 7 International international JJ 10_1101-2020_11_24_390039 9 8 licensemade licensemade NN 10_1101-2020_11_24_390039 9 9 available available JJ 10_1101-2020_11_24_390039 9 10 under under IN 10_1101-2020_11_24_390039 9 11 a a DT 10_1101-2020_11_24_390039 9 12 ( ( -LRB- 10_1101-2020_11_24_390039 9 13 which which WDT 10_1101-2020_11_24_390039 9 14 was be VBD 10_1101-2020_11_24_390039 9 15 not not RB 10_1101-2020_11_24_390039 9 16 certified certify VBN 10_1101-2020_11_24_390039 9 17 by by IN 10_1101-2020_11_24_390039 9 18 peer peer NN 10_1101-2020_11_24_390039 9 19 review review NN 10_1101-2020_11_24_390039 9 20 ) ) -RRB- 10_1101-2020_11_24_390039 9 21 is be VBZ 10_1101-2020_11_24_390039 9 22 the the DT 10_1101-2020_11_24_390039 9 23 author author NN 10_1101-2020_11_24_390039 9 24 / / SYM 10_1101-2020_11_24_390039 9 25 funder funder NN 10_1101-2020_11_24_390039 9 26 , , , 10_1101-2020_11_24_390039 9 27 who who WP 10_1101-2020_11_24_390039 9 28 has have VBZ 10_1101-2020_11_24_390039 9 29 granted grant VBN 10_1101-2020_11_24_390039 9 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 9 31 a a DT 10_1101-2020_11_24_390039 9 32 license license NN 10_1101-2020_11_24_390039 9 33 to to TO 10_1101-2020_11_24_390039 9 34 display display VB 10_1101-2020_11_24_390039 9 35 the the DT 10_1101-2020_11_24_390039 9 36 preprint preprint NN 10_1101-2020_11_24_390039 9 37 in in IN 10_1101-2020_11_24_390039 9 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 9 39 . . . 10_1101-2020_11_24_390039 10 1 It -PRON- PRP 10_1101-2020_11_24_390039 10 2 is be VBZ 10_1101-2020_11_24_390039 10 3 The the DT 10_1101-2020_11_24_390039 10 4 copyright copyright NN 10_1101-2020_11_24_390039 10 5 holder holder NN 10_1101-2020_11_24_390039 10 6 for for IN 10_1101-2020_11_24_390039 10 7 this this DT 10_1101-2020_11_24_390039 10 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 10 9 version version NN 10_1101-2020_11_24_390039 10 10 posted post VBD 10_1101-2020_11_24_390039 10 11 January January NNP 10_1101-2020_11_24_390039 10 12 5 5 CD 10_1101-2020_11_24_390039 10 13 , , , 10_1101-2020_11_24_390039 10 14 2021 2021 CD 10_1101-2020_11_24_390039 10 15 . . . 10_1101-2020_11_24_390039 10 16 ; ; : 10_1101-2020_11_24_390039 10 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 10 18 : : : 10_1101-2020_11_24_390039 10 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 10 20 preprint preprint JJ 10_1101-2020_11_24_390039 10 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 10 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 10 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 10 24 - - HYPH 10_1101-2020_11_24_390039 10 25 F F NNP 10_1101-2020_11_24_390039 10 26 as as IN 10_1101-2020_11_24_390039 10 27 a a DT 10_1101-2020_11_24_390039 10 28 potential potential JJ 10_1101-2020_11_24_390039 10 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 10 30 agent agent NN 10_1101-2020_11_24_390039 10 31 Page Page NNP 10_1101-2020_11_24_390039 10 32 3 3 CD 10_1101-2020_11_24_390039 10 33 of of IN 10_1101-2020_11_24_390039 10 34 21 21 CD 10_1101-2020_11_24_390039 10 35 Introduction introduction NN 10_1101-2020_11_24_390039 10 36 Many many JJ 10_1101-2020_11_24_390039 10 37 viruses virus NNS 10_1101-2020_11_24_390039 10 38 , , , 10_1101-2020_11_24_390039 10 39 including include VBG 10_1101-2020_11_24_390039 10 40 SARS SARS NNP 10_1101-2020_11_24_390039 10 41 - - HYPH 10_1101-2020_11_24_390039 10 42 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 10 43 ( ( -LRB- 10_1101-2020_11_24_390039 10 44 Zhou Zhou NNP 10_1101-2020_11_24_390039 10 45 et et FW 10_1101-2020_11_24_390039 10 46 al al NNP 10_1101-2020_11_24_390039 10 47 . . NNP 10_1101-2020_11_24_390039 10 48 , , , 10_1101-2020_11_24_390039 10 49 2020 2020 CD 10_1101-2020_11_24_390039 10 50 ; ; : 10_1101-2020_11_24_390039 10 51 Zhu Zhu NNP 10_1101-2020_11_24_390039 10 52 et et FW 10_1101-2020_11_24_390039 10 53 al al NNP 10_1101-2020_11_24_390039 10 54 . . NNP 10_1101-2020_11_24_390039 10 55 , , , 10_1101-2020_11_24_390039 10 56 2020 2020 CD 10_1101-2020_11_24_390039 10 57 ) ) -RRB- 10_1101-2020_11_24_390039 10 58 ( ( -LRB- 10_1101-2020_11_24_390039 10 59 Fig fig NN 10_1101-2020_11_24_390039 10 60 . . . 10_1101-2020_11_24_390039 11 1 1A 1a LS 10_1101-2020_11_24_390039 11 2 ) ) -RRB- 10_1101-2020_11_24_390039 11 3 , , , 10_1101-2020_11_24_390039 11 4 hijack hijack VB 10_1101-2020_11_24_390039 11 5 the the DT 10_1101-2020_11_24_390039 11 6 host host NN 10_1101-2020_11_24_390039 11 7 cell cell NN 10_1101-2020_11_24_390039 11 8 secretory secretory NN 10_1101-2020_11_24_390039 11 9 pathway pathway NN 10_1101-2020_11_24_390039 11 10 to to TO 10_1101-2020_11_24_390039 11 11 correctly correctly RB 10_1101-2020_11_24_390039 11 12 synthesise synthesise VB 10_1101-2020_11_24_390039 11 13 , , , 10_1101-2020_11_24_390039 11 14 fold fold VB 10_1101-2020_11_24_390039 11 15 and and CC 10_1101-2020_11_24_390039 11 16 assemble assemble VB 10_1101-2020_11_24_390039 11 17 important important JJ 10_1101-2020_11_24_390039 11 18 viral viral JJ 10_1101-2020_11_24_390039 11 19 proteins protein NNS 10_1101-2020_11_24_390039 11 20 ( ( -LRB- 10_1101-2020_11_24_390039 11 21 Bojkova Bojkova NNP 10_1101-2020_11_24_390039 11 22 et et NNP 10_1101-2020_11_24_390039 11 23 al al NNP 10_1101-2020_11_24_390039 11 24 . . NNP 10_1101-2020_11_24_390039 11 25 , , , 10_1101-2020_11_24_390039 11 26 2020 2020 CD 10_1101-2020_11_24_390039 11 27 ; ; : 10_1101-2020_11_24_390039 11 28 Gordon Gordon NNP 10_1101-2020_11_24_390039 11 29 et et FW 10_1101-2020_11_24_390039 11 30 al al NNP 10_1101-2020_11_24_390039 11 31 . . NNP 10_1101-2020_11_24_390039 11 32 , , , 10_1101-2020_11_24_390039 11 33 2020 2020 CD 10_1101-2020_11_24_390039 11 34 ; ; : 10_1101-2020_11_24_390039 11 35 Sicari Sicari NNP 10_1101-2020_11_24_390039 11 36 et et FW 10_1101-2020_11_24_390039 11 37 al al NNP 10_1101-2020_11_24_390039 11 38 . . NNP 10_1101-2020_11_24_390039 11 39 , , , 10_1101-2020_11_24_390039 11 40 2020 2020 CD 10_1101-2020_11_24_390039 11 41 ) ) -RRB- 10_1101-2020_11_24_390039 11 42 . . . 10_1101-2020_11_24_390039 12 1 Hence hence RB 10_1101-2020_11_24_390039 12 2 , , , 10_1101-2020_11_24_390039 12 3 small small JJ 10_1101-2020_11_24_390039 12 4 molecule molecule NN 10_1101-2020_11_24_390039 12 5 inhibitors inhibitor NNS 10_1101-2020_11_24_390039 12 6 of of IN 10_1101-2020_11_24_390039 12 7 Sec61-mediated sec61-mediate VBN 10_1101-2020_11_24_390039 12 8 co co JJ 10_1101-2020_11_24_390039 12 9 - - JJ 10_1101-2020_11_24_390039 12 10 translational translational JJ 10_1101-2020_11_24_390039 12 11 protein protein NN 10_1101-2020_11_24_390039 12 12 entry entry NN 10_1101-2020_11_24_390039 12 13 into into IN 10_1101-2020_11_24_390039 12 14 the the DT 10_1101-2020_11_24_390039 12 15 endoplasmic endoplasmic JJ 10_1101-2020_11_24_390039 12 16 reticulum reticulum NN 10_1101-2020_11_24_390039 12 17 ( ( -LRB- 10_1101-2020_11_24_390039 12 18 ER ER NNP 10_1101-2020_11_24_390039 12 19 ) ) -RRB- 10_1101-2020_11_24_390039 12 20 ( ( -LRB- 10_1101-2020_11_24_390039 12 21 Luesch Luesch NNP 10_1101-2020_11_24_390039 12 22 and and CC 10_1101-2020_11_24_390039 12 23 Paavilainen Paavilainen NNP 10_1101-2020_11_24_390039 12 24 , , , 10_1101-2020_11_24_390039 12 25 2020 2020 CD 10_1101-2020_11_24_390039 12 26 ) ) -RRB- 10_1101-2020_11_24_390039 12 27 have have VBP 10_1101-2020_11_24_390039 12 28 potential potential JJ 10_1101-2020_11_24_390039 12 29 as as IN 10_1101-2020_11_24_390039 12 30 broad broad JJ 10_1101-2020_11_24_390039 12 31 - - HYPH 10_1101-2020_11_24_390039 12 32 spectrum spectrum NN 10_1101-2020_11_24_390039 12 33 antivirals antiviral NNS 10_1101-2020_11_24_390039 12 34 ( ( -LRB- 10_1101-2020_11_24_390039 12 35 Heaton Heaton NNP 10_1101-2020_11_24_390039 12 36 et et FW 10_1101-2020_11_24_390039 12 37 al al NNP 10_1101-2020_11_24_390039 12 38 . . NNP 10_1101-2020_11_24_390039 12 39 , , , 10_1101-2020_11_24_390039 12 40 2016 2016 CD 10_1101-2020_11_24_390039 12 41 ; ; : 10_1101-2020_11_24_390039 12 42 Shah Shah NNP 10_1101-2020_11_24_390039 12 43 et et NNP 10_1101-2020_11_24_390039 12 44 al al NNP 10_1101-2020_11_24_390039 12 45 . . NNP 10_1101-2020_11_24_390039 12 46 , , , 10_1101-2020_11_24_390039 12 47 2018 2018 CD 10_1101-2020_11_24_390039 12 48 ) ) -RRB- 10_1101-2020_11_24_390039 12 49 . . . 10_1101-2020_11_24_390039 13 1 Such such JJ 10_1101-2020_11_24_390039 13 2 inhibitors inhibitor NNS 10_1101-2020_11_24_390039 13 3 offer offer VBP 10_1101-2020_11_24_390039 13 4 a a DT 10_1101-2020_11_24_390039 13 5 dual dual JJ 10_1101-2020_11_24_390039 13 6 approach approach NN 10_1101-2020_11_24_390039 13 7 ; ; : 10_1101-2020_11_24_390039 13 8 first first RB 10_1101-2020_11_24_390039 13 9 , , , 10_1101-2020_11_24_390039 13 10 by by IN 10_1101-2020_11_24_390039 13 11 directly directly RB 10_1101-2020_11_24_390039 13 12 inhibiting inhibit VBG 10_1101-2020_11_24_390039 13 13 production production NN 10_1101-2020_11_24_390039 13 14 of of IN 10_1101-2020_11_24_390039 13 15 key key JJ 10_1101-2020_11_24_390039 13 16 viral viral JJ 10_1101-2020_11_24_390039 13 17 proteins protein NNS 10_1101-2020_11_24_390039 13 18 and and CC 10_1101-2020_11_24_390039 13 19 , , , 10_1101-2020_11_24_390039 13 20 second second RB 10_1101-2020_11_24_390039 13 21 , , , 10_1101-2020_11_24_390039 13 22 by by IN 10_1101-2020_11_24_390039 13 23 reducing reduce VBG 10_1101-2020_11_24_390039 13 24 levels level NNS 10_1101-2020_11_24_390039 13 25 of of IN 10_1101-2020_11_24_390039 13 26 host host NN 10_1101-2020_11_24_390039 13 27 proteins protein NNS 10_1101-2020_11_24_390039 13 28 co co VBD 10_1101-2020_11_24_390039 13 29 - - JJ 10_1101-2020_11_24_390039 13 30 opted opted JJ 10_1101-2020_11_24_390039 13 31 during during IN 10_1101-2020_11_24_390039 13 32 viral viral JJ 10_1101-2020_11_24_390039 13 33 infection infection NN 10_1101-2020_11_24_390039 13 34 . . . 10_1101-2020_11_24_390039 14 1 Hence hence RB 10_1101-2020_11_24_390039 14 2 , , , 10_1101-2020_11_24_390039 14 3 human human JJ 10_1101-2020_11_24_390039 14 4 angiotensin angiotensin NN 10_1101-2020_11_24_390039 14 5 - - HYPH 10_1101-2020_11_24_390039 14 6 converting convert VBG 10_1101-2020_11_24_390039 14 7 enzyme enzyme NNS 10_1101-2020_11_24_390039 14 8 2 2 CD 10_1101-2020_11_24_390039 14 9 ( ( -LRB- 10_1101-2020_11_24_390039 14 10 ACE2 ACE2 NNP 10_1101-2020_11_24_390039 14 11 ) ) -RRB- 10_1101-2020_11_24_390039 14 12 is be VBZ 10_1101-2020_11_24_390039 14 13 an an DT 10_1101-2020_11_24_390039 14 14 important important JJ 10_1101-2020_11_24_390039 14 15 host host NN 10_1101-2020_11_24_390039 14 16 cell cell NN 10_1101-2020_11_24_390039 14 17 receptor receptor NN 10_1101-2020_11_24_390039 14 18 for for IN 10_1101-2020_11_24_390039 14 19 SARS SARS NNP 10_1101-2020_11_24_390039 14 20 - - HYPH 10_1101-2020_11_24_390039 14 21 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 14 22 viral viral JJ 10_1101-2020_11_24_390039 14 23 entry entry NN 10_1101-2020_11_24_390039 14 24 ( ( -LRB- 10_1101-2020_11_24_390039 14 25 Cantuti Cantuti NNP 10_1101-2020_11_24_390039 14 26 - - HYPH 10_1101-2020_11_24_390039 14 27 Castelvetri Castelvetri NNP 10_1101-2020_11_24_390039 14 28 et et FW 10_1101-2020_11_24_390039 14 29 al al NNP 10_1101-2020_11_24_390039 14 30 . . NNP 10_1101-2020_11_24_390039 14 31 , , , 10_1101-2020_11_24_390039 14 32 2020 2020 CD 10_1101-2020_11_24_390039 14 33 ; ; : 10_1101-2020_11_24_390039 14 34 Daly Daly NNP 10_1101-2020_11_24_390039 14 35 et et NNP 10_1101-2020_11_24_390039 14 36 al al NNP 10_1101-2020_11_24_390039 14 37 . . NNP 10_1101-2020_11_24_390039 14 38 , , , 10_1101-2020_11_24_390039 14 39 2020 2020 CD 10_1101-2020_11_24_390039 14 40 ; ; : 10_1101-2020_11_24_390039 14 41 Walls Walls NNP 10_1101-2020_11_24_390039 14 42 et et FW 10_1101-2020_11_24_390039 14 43 al al NNP 10_1101-2020_11_24_390039 14 44 , , , 10_1101-2020_11_24_390039 14 45 2020 2020 CD 10_1101-2020_11_24_390039 14 46 ) ) -RRB- 10_1101-2020_11_24_390039 14 47 synthesised synthesise VBD 10_1101-2020_11_24_390039 14 48 at at IN 10_1101-2020_11_24_390039 14 49 the the DT 10_1101-2020_11_24_390039 14 50 ER ER NNP 10_1101-2020_11_24_390039 14 51 prior prior RB 10_1101-2020_11_24_390039 14 52 to to IN 10_1101-2020_11_24_390039 14 53 its -PRON- PRP$ 10_1101-2020_11_24_390039 14 54 trafficking trafficking NN 10_1101-2020_11_24_390039 14 55 to to IN 10_1101-2020_11_24_390039 14 56 the the DT 10_1101-2020_11_24_390039 14 57 plasma plasma NN 10_1101-2020_11_24_390039 14 58 membrane membrane NN 10_1101-2020_11_24_390039 14 59 ( ( -LRB- 10_1101-2020_11_24_390039 14 60 Warner Warner NNP 10_1101-2020_11_24_390039 14 61 et et NNP 10_1101-2020_11_24_390039 14 62 al al NNP 10_1101-2020_11_24_390039 14 63 . . NNP 10_1101-2020_11_24_390039 14 64 , , , 10_1101-2020_11_24_390039 14 65 2005 2005 CD 10_1101-2020_11_24_390039 14 66 ) ) -RRB- 10_1101-2020_11_24_390039 14 67 . . . 10_1101-2020_11_24_390039 15 1 Our -PRON- PRP$ 10_1101-2020_11_24_390039 15 2 recent recent JJ 10_1101-2020_11_24_390039 15 3 studies study NNS 10_1101-2020_11_24_390039 15 4 show show VBP 10_1101-2020_11_24_390039 15 5 that that IN 10_1101-2020_11_24_390039 15 6 ipomoeassin ipomoeassin NNP 10_1101-2020_11_24_390039 15 7 - - HYPH 10_1101-2020_11_24_390039 15 8 F F NNP 10_1101-2020_11_24_390039 15 9 ( ( -LRB- 10_1101-2020_11_24_390039 15 10 Ipom Ipom NNP 10_1101-2020_11_24_390039 15 11 - - HYPH 10_1101-2020_11_24_390039 15 12 F F NNP 10_1101-2020_11_24_390039 15 13 ) ) -RRB- 10_1101-2020_11_24_390039 15 14 ( ( -LRB- 10_1101-2020_11_24_390039 15 15 Fig fig NN 10_1101-2020_11_24_390039 15 16 . . . 10_1101-2020_11_24_390039 16 1 1B 1B NNP 10_1101-2020_11_24_390039 16 2 ) ) -RRB- 10_1101-2020_11_24_390039 16 3 is be VBZ 10_1101-2020_11_24_390039 16 4 a a DT 10_1101-2020_11_24_390039 16 5 potent potent JJ 10_1101-2020_11_24_390039 16 6 and and CC 10_1101-2020_11_24_390039 16 7 selective selective JJ 10_1101-2020_11_24_390039 16 8 inhibitor inhibitor NN 10_1101-2020_11_24_390039 16 9 of of IN 10_1101-2020_11_24_390039 16 10 Sec61-mediated sec61-mediate VBN 10_1101-2020_11_24_390039 16 11 protein protein NN 10_1101-2020_11_24_390039 16 12 translocation translocation NN 10_1101-2020_11_24_390039 16 13 at at IN 10_1101-2020_11_24_390039 16 14 the the DT 10_1101-2020_11_24_390039 16 15 ER ER NNP 10_1101-2020_11_24_390039 16 16 membrane membrane NN 10_1101-2020_11_24_390039 16 17 ( ( -LRB- 10_1101-2020_11_24_390039 16 18 Zong Zong NNP 10_1101-2020_11_24_390039 16 19 et et NNP 10_1101-2020_11_24_390039 16 20 al al NNP 10_1101-2020_11_24_390039 16 21 . . NNP 10_1101-2020_11_24_390039 16 22 , , , 10_1101-2020_11_24_390039 16 23 2019 2019 CD 10_1101-2020_11_24_390039 16 24 ; ; : 10_1101-2020_11_24_390039 16 25 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 16 26 et et NNP 10_1101-2020_11_24_390039 16 27 al al NNP 10_1101-2020_11_24_390039 16 28 . . NNP 10_1101-2020_11_24_390039 16 29 , , , 10_1101-2020_11_24_390039 16 30 2020 2020 CD 10_1101-2020_11_24_390039 16 31 submitted submit VBN 10_1101-2020_11_24_390039 16 32 ) ) -RRB- 10_1101-2020_11_24_390039 16 33 . . . 10_1101-2020_11_24_390039 17 1 Given give VBN 10_1101-2020_11_24_390039 17 2 that that IN 10_1101-2020_11_24_390039 17 3 SARS SARS NNP 10_1101-2020_11_24_390039 17 4 - - HYPH 10_1101-2020_11_24_390039 17 5 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 17 6 membrane membrane NN 10_1101-2020_11_24_390039 17 7 proteins protein VBZ 10_1101-2020_11_24_390039 17 8 likely likely RB 10_1101-2020_11_24_390039 17 9 co co VBD 10_1101-2020_11_24_390039 17 10 - - JJ 10_1101-2020_11_24_390039 17 11 opt opt JJ 10_1101-2020_11_24_390039 17 12 host host NN 10_1101-2020_11_24_390039 17 13 mechanisms mechanism NNS 10_1101-2020_11_24_390039 17 14 of of IN 10_1101-2020_11_24_390039 17 15 ER ER NNP 10_1101-2020_11_24_390039 17 16 entry entry NN 10_1101-2020_11_24_390039 17 17 ( ( -LRB- 10_1101-2020_11_24_390039 17 18 cf cf NN 10_1101-2020_11_24_390039 17 19 . . . 10_1101-2020_11_24_390039 18 1 Gordon Gordon NNP 10_1101-2020_11_24_390039 18 2 et et FW 10_1101-2020_11_24_390039 18 3 al al NNP 10_1101-2020_11_24_390039 18 4 . . NNP 10_1101-2020_11_24_390039 18 5 , , , 10_1101-2020_11_24_390039 18 6 2020 2020 CD 10_1101-2020_11_24_390039 18 7 ; ; : 10_1101-2020_11_24_390039 18 8 Sicari Sicari NNP 10_1101-2020_11_24_390039 18 9 et et FW 10_1101-2020_11_24_390039 18 10 al al NNP 10_1101-2020_11_24_390039 18 11 . . NNP 10_1101-2020_11_24_390039 18 12 , , , 10_1101-2020_11_24_390039 18 13 2020 2020 CD 10_1101-2020_11_24_390039 18 14 ) ) -RRB- 10_1101-2020_11_24_390039 18 15 , , , 10_1101-2020_11_24_390039 18 16 we -PRON- PRP 10_1101-2020_11_24_390039 18 17 concluded conclude VBD 10_1101-2020_11_24_390039 18 18 that that IN 10_1101-2020_11_24_390039 18 19 their -PRON- PRP$ 10_1101-2020_11_24_390039 18 20 sensitivity sensitivity NN 10_1101-2020_11_24_390039 18 21 to to IN 10_1101-2020_11_24_390039 18 22 Ipom Ipom NNP 10_1101-2020_11_24_390039 18 23 - - HYPH 10_1101-2020_11_24_390039 18 24 F F NNP 10_1101-2020_11_24_390039 18 25 would would MD 10_1101-2020_11_24_390039 18 26 likely likely RB 10_1101-2020_11_24_390039 18 27 be be VB 10_1101-2020_11_24_390039 18 28 comparable comparable JJ 10_1101-2020_11_24_390039 18 29 to to IN 10_1101-2020_11_24_390039 18 30 that that DT 10_1101-2020_11_24_390039 18 31 of of IN 10_1101-2020_11_24_390039 18 32 endogenous endogenous JJ 10_1101-2020_11_24_390039 18 33 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 18 34 clients client NNS 10_1101-2020_11_24_390039 18 35 ( ( -LRB- 10_1101-2020_11_24_390039 18 36 Fig fig NN 10_1101-2020_11_24_390039 18 37 . . . 10_1101-2020_11_24_390039 19 1 1C 1C NNP 10_1101-2020_11_24_390039 19 2 ; ; : 10_1101-2020_11_24_390039 19 3 see see VB 10_1101-2020_11_24_390039 19 4 also also RB 10_1101-2020_11_24_390039 19 5 Zong Zong NNP 10_1101-2020_11_24_390039 19 6 et et NNP 10_1101-2020_11_24_390039 19 7 al al NNP 10_1101-2020_11_24_390039 19 8 . . NNP 10_1101-2020_11_24_390039 19 9 , , , 10_1101-2020_11_24_390039 19 10 2019 2019 CD 10_1101-2020_11_24_390039 19 11 ; ; : 10_1101-2020_11_24_390039 19 12 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 19 13 et et NNP 10_1101-2020_11_24_390039 19 14 al al NNP 10_1101-2020_11_24_390039 19 15 . . NNP 10_1101-2020_11_24_390039 19 16 , , , 10_1101-2020_11_24_390039 19 17 2020 2020 CD 10_1101-2020_11_24_390039 19 18 submitted submit VBN 10_1101-2020_11_24_390039 19 19 ) ) -RRB- 10_1101-2020_11_24_390039 19 20 . . . 10_1101-2020_11_24_390039 20 1 We -PRON- PRP 10_1101-2020_11_24_390039 20 2 , , , 10_1101-2020_11_24_390039 20 3 therefore therefore RB 10_1101-2020_11_24_390039 20 4 , , , 10_1101-2020_11_24_390039 20 5 evaluated evaluate VBD 10_1101-2020_11_24_390039 20 6 the the DT 10_1101-2020_11_24_390039 20 7 effects effect NNS 10_1101-2020_11_24_390039 20 8 of of IN 10_1101-2020_11_24_390039 20 9 Ipom Ipom NNP 10_1101-2020_11_24_390039 20 10 - - HYPH 10_1101-2020_11_24_390039 20 11 F F NNP 10_1101-2020_11_24_390039 20 12 on on IN 10_1101-2020_11_24_390039 20 13 SARS SARS NNP 10_1101-2020_11_24_390039 20 14 - - HYPH 10_1101-2020_11_24_390039 20 15 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 20 16 proteins protein NNS 10_1101-2020_11_24_390039 20 17 containing contain VBG 10_1101-2020_11_24_390039 20 18 hydrophobic hydrophobic NN 10_1101-2020_11_24_390039 20 19 ER ER NNP 10_1101-2020_11_24_390039 20 20 targeting targeting NN 10_1101-2020_11_24_390039 20 21 signals signal NNS 10_1101-2020_11_24_390039 20 22 ( ( -LRB- 10_1101-2020_11_24_390039 20 23 Fig fig NN 10_1101-2020_11_24_390039 20 24 . . . 10_1101-2020_11_24_390039 21 1 1D 1D NNP 10_1101-2020_11_24_390039 21 2 ) ) -RRB- 10_1101-2020_11_24_390039 21 3 . . . 10_1101-2020_11_24_390039 22 1 The the DT 10_1101-2020_11_24_390039 22 2 in in FW 10_1101-2020_11_24_390039 22 3 vitro vitro FW 10_1101-2020_11_24_390039 22 4 membrane membrane NN 10_1101-2020_11_24_390039 22 5 insertion insertion NN 10_1101-2020_11_24_390039 22 6 of of IN 10_1101-2020_11_24_390039 22 7 the the DT 10_1101-2020_11_24_390039 22 8 viral viral JJ 10_1101-2020_11_24_390039 22 9 spike spike NN 10_1101-2020_11_24_390039 22 10 ( ( -LRB- 10_1101-2020_11_24_390039 22 11 S S NNP 10_1101-2020_11_24_390039 22 12 ) ) -RRB- 10_1101-2020_11_24_390039 22 13 protein protein NN 10_1101-2020_11_24_390039 22 14 and and CC 10_1101-2020_11_24_390039 22 15 membrane membrane NN 10_1101-2020_11_24_390039 22 16 translocation translocation NN 10_1101-2020_11_24_390039 22 17 of of IN 10_1101-2020_11_24_390039 22 18 the the DT 10_1101-2020_11_24_390039 22 19 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 22 20 protein protein NN 10_1101-2020_11_24_390039 22 21 are be VBP 10_1101-2020_11_24_390039 22 22 both both DT 10_1101-2020_11_24_390039 22 23 strongly strongly RB 10_1101-2020_11_24_390039 22 24 inhibited inhibit VBN 10_1101-2020_11_24_390039 22 25 by by IN 10_1101-2020_11_24_390039 22 26 Ipom- Ipom- NNP 10_1101-2020_11_24_390039 22 27 F F NNP 10_1101-2020_11_24_390039 22 28 , , , 10_1101-2020_11_24_390039 22 29 whilst whilst IN 10_1101-2020_11_24_390039 22 30 several several JJ 10_1101-2020_11_24_390039 22 31 other other JJ 10_1101-2020_11_24_390039 22 32 viral viral JJ 10_1101-2020_11_24_390039 22 33 membrane membrane NN 10_1101-2020_11_24_390039 22 34 proteins protein NNS 10_1101-2020_11_24_390039 22 35 are be VBP 10_1101-2020_11_24_390039 22 36 unaffected unaffected JJ 10_1101-2020_11_24_390039 22 37 ( ( -LRB- 10_1101-2020_11_24_390039 22 38 Fig fig NN 10_1101-2020_11_24_390039 22 39 . . . 10_1101-2020_11_24_390039 23 1 2 2 LS 10_1101-2020_11_24_390039 23 2 ) ) -RRB- 10_1101-2020_11_24_390039 23 3 . . . 10_1101-2020_11_24_390039 24 1 Likewise likewise RB 10_1101-2020_11_24_390039 24 2 , , , 10_1101-2020_11_24_390039 24 3 the the DT 10_1101-2020_11_24_390039 24 4 ER ER NNP 10_1101-2020_11_24_390039 24 5 integration integration NN 10_1101-2020_11_24_390039 24 6 of of IN 10_1101-2020_11_24_390039 24 7 ACE2 ACE2 NNP 10_1101-2020_11_24_390039 24 8 , , , 10_1101-2020_11_24_390039 24 9 an an DT 10_1101-2020_11_24_390039 24 10 important important JJ 10_1101-2020_11_24_390039 24 11 host host NN 10_1101-2020_11_24_390039 24 12 receptor receptor NN 10_1101-2020_11_24_390039 24 13 for for IN 10_1101-2020_11_24_390039 24 14 SARS SARS NNP 10_1101-2020_11_24_390039 24 15 - - HYPH 10_1101-2020_11_24_390039 24 16 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 24 17 ( ( -LRB- 10_1101-2020_11_24_390039 24 18 Walls Walls NNP 10_1101-2020_11_24_390039 24 19 et et FW 10_1101-2020_11_24_390039 24 20 al al NNP 10_1101-2020_11_24_390039 24 21 . . NNP 10_1101-2020_11_24_390039 24 22 , , , 10_1101-2020_11_24_390039 24 23 2020 2020 CD 10_1101-2020_11_24_390039 24 24 ) ) -RRB- 10_1101-2020_11_24_390039 24 25 , , , 10_1101-2020_11_24_390039 24 26 is be VBZ 10_1101-2020_11_24_390039 24 27 highly highly RB 10_1101-2020_11_24_390039 24 28 sensitive sensitive JJ 10_1101-2020_11_24_390039 24 29 to to IN 10_1101-2020_11_24_390039 24 30 Ipom Ipom NNP 10_1101-2020_11_24_390039 24 31 - - HYPH 10_1101-2020_11_24_390039 24 32 F F NNP 10_1101-2020_11_24_390039 24 33 ( ( -LRB- 10_1101-2020_11_24_390039 24 34 Fig Fig NNP 10_1101-2020_11_24_390039 24 35 . . . 10_1101-2020_11_24_390039 25 1 2 2 LS 10_1101-2020_11_24_390039 25 2 ) ) -RRB- 10_1101-2020_11_24_390039 25 3 . . . 10_1101-2020_11_24_390039 26 1 We -PRON- PRP 10_1101-2020_11_24_390039 26 2 show show VBP 10_1101-2020_11_24_390039 26 3 that that IN 10_1101-2020_11_24_390039 26 4 the the DT 10_1101-2020_11_24_390039 26 5 principle principle JJ 10_1101-2020_11_24_390039 26 6 molecular molecular JJ 10_1101-2020_11_24_390039 26 7 basis basis NN 10_1101-2020_11_24_390039 26 8 for for IN 10_1101-2020_11_24_390039 26 9 the the DT 10_1101-2020_11_24_390039 26 10 Ipom Ipom NNP 10_1101-2020_11_24_390039 26 11 - - HYPH 10_1101-2020_11_24_390039 26 12 F F NNP 10_1101-2020_11_24_390039 26 13 sensitivity sensitivity NN 10_1101-2020_11_24_390039 26 14 of of IN 10_1101-2020_11_24_390039 26 15 SARS- SARS- NNP 10_1101-2020_11_24_390039 26 16 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 26 17 proteins protein NNS 10_1101-2020_11_24_390039 26 18 is be VBZ 10_1101-2020_11_24_390039 26 19 their -PRON- PRP$ 10_1101-2020_11_24_390039 26 20 dependence dependence NN 10_1101-2020_11_24_390039 26 21 on on IN 10_1101-2020_11_24_390039 26 22 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 26 23 , , , 10_1101-2020_11_24_390039 26 24 as as IN 10_1101-2020_11_24_390039 26 25 dictated dictate VBN 10_1101-2020_11_24_390039 26 26 by by IN 10_1101-2020_11_24_390039 26 27 their -PRON- PRP$ 10_1101-2020_11_24_390039 26 28 individual individual JJ 10_1101-2020_11_24_390039 26 29 structural structural JJ 10_1101-2020_11_24_390039 26 30 features feature NNS 10_1101-2020_11_24_390039 26 31 and and CC 10_1101-2020_11_24_390039 26 32 membrane membrane NN 10_1101-2020_11_24_390039 26 33 topologies topology NNS 10_1101-2020_11_24_390039 26 34 ( ( -LRB- 10_1101-2020_11_24_390039 26 35 Fig fig NN 10_1101-2020_11_24_390039 26 36 . . . 10_1101-2020_11_24_390039 27 1 3 3 LS 10_1101-2020_11_24_390039 27 2 ) ) -RRB- 10_1101-2020_11_24_390039 27 3 . . . 10_1101-2020_11_24_390039 28 1 Taken take VBN 10_1101-2020_11_24_390039 28 2 together together RB 10_1101-2020_11_24_390039 28 3 , , , 10_1101-2020_11_24_390039 28 4 our -PRON- PRP$ 10_1101-2020_11_24_390039 28 5 in in FW 10_1101-2020_11_24_390039 28 6 vitro vitro FW 10_1101-2020_11_24_390039 28 7 study study NN 10_1101-2020_11_24_390039 28 8 of of IN 10_1101-2020_11_24_390039 28 9 SARS SARS NNP 10_1101-2020_11_24_390039 28 10 - - HYPH 10_1101-2020_11_24_390039 28 11 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 28 12 protein protein NN 10_1101-2020_11_24_390039 28 13 synthesis synthesis NN 10_1101-2020_11_24_390039 28 14 at at IN 10_1101-2020_11_24_390039 28 15 the the DT 10_1101-2020_11_24_390039 28 16 ER ER NNP 10_1101-2020_11_24_390039 28 17 highlights highlight VBZ 10_1101-2020_11_24_390039 28 18 Ipom Ipom NNP 10_1101-2020_11_24_390039 28 19 - - HYPH 10_1101-2020_11_24_390039 28 20 F F NNP 10_1101-2020_11_24_390039 28 21 as as IN 10_1101-2020_11_24_390039 28 22 a a DT 10_1101-2020_11_24_390039 28 23 promising promise VBG 10_1101-2020_11_24_390039 28 24 candidate candidate NN 10_1101-2020_11_24_390039 28 25 for for IN 10_1101-2020_11_24_390039 28 26 the the DT 10_1101-2020_11_24_390039 28 27 development development NN 10_1101-2020_11_24_390039 28 28 of of IN 10_1101-2020_11_24_390039 28 29 a a DT 10_1101-2020_11_24_390039 28 30 broad broad JJ 10_1101-2020_11_24_390039 28 31 - - HYPH 10_1101-2020_11_24_390039 28 32 spectrum spectrum NN 10_1101-2020_11_24_390039 28 33 , , , 10_1101-2020_11_24_390039 28 34 host host NN 10_1101-2020_11_24_390039 28 35 - - HYPH 10_1101-2020_11_24_390039 28 36 targeting targeting JJ 10_1101-2020_11_24_390039 28 37 , , , 10_1101-2020_11_24_390039 28 38 antiviral antiviral JJ 10_1101-2020_11_24_390039 28 39 agent agent NN 10_1101-2020_11_24_390039 28 40 . . . 10_1101-2020_11_24_390039 29 1 .CC .CC NFP 10_1101-2020_11_24_390039 29 2 - - : 10_1101-2020_11_24_390039 29 3 BY by IN 10_1101-2020_11_24_390039 29 4 - - HYPH 10_1101-2020_11_24_390039 29 5 ND ND NNP 10_1101-2020_11_24_390039 29 6 4.0 4.0 CD 10_1101-2020_11_24_390039 29 7 International international JJ 10_1101-2020_11_24_390039 29 8 licensemade licensemade NN 10_1101-2020_11_24_390039 29 9 available available JJ 10_1101-2020_11_24_390039 29 10 under under IN 10_1101-2020_11_24_390039 29 11 a a DT 10_1101-2020_11_24_390039 29 12 ( ( -LRB- 10_1101-2020_11_24_390039 29 13 which which WDT 10_1101-2020_11_24_390039 29 14 was be VBD 10_1101-2020_11_24_390039 29 15 not not RB 10_1101-2020_11_24_390039 29 16 certified certify VBN 10_1101-2020_11_24_390039 29 17 by by IN 10_1101-2020_11_24_390039 29 18 peer peer NN 10_1101-2020_11_24_390039 29 19 review review NN 10_1101-2020_11_24_390039 29 20 ) ) -RRB- 10_1101-2020_11_24_390039 29 21 is be VBZ 10_1101-2020_11_24_390039 29 22 the the DT 10_1101-2020_11_24_390039 29 23 author author NN 10_1101-2020_11_24_390039 29 24 / / SYM 10_1101-2020_11_24_390039 29 25 funder funder NN 10_1101-2020_11_24_390039 29 26 , , , 10_1101-2020_11_24_390039 29 27 who who WP 10_1101-2020_11_24_390039 29 28 has have VBZ 10_1101-2020_11_24_390039 29 29 granted grant VBN 10_1101-2020_11_24_390039 29 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 29 31 a a DT 10_1101-2020_11_24_390039 29 32 license license NN 10_1101-2020_11_24_390039 29 33 to to TO 10_1101-2020_11_24_390039 29 34 display display VB 10_1101-2020_11_24_390039 29 35 the the DT 10_1101-2020_11_24_390039 29 36 preprint preprint NN 10_1101-2020_11_24_390039 29 37 in in IN 10_1101-2020_11_24_390039 29 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 29 39 . . . 10_1101-2020_11_24_390039 30 1 It -PRON- PRP 10_1101-2020_11_24_390039 30 2 is be VBZ 10_1101-2020_11_24_390039 30 3 The the DT 10_1101-2020_11_24_390039 30 4 copyright copyright NN 10_1101-2020_11_24_390039 30 5 holder holder NN 10_1101-2020_11_24_390039 30 6 for for IN 10_1101-2020_11_24_390039 30 7 this this DT 10_1101-2020_11_24_390039 30 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 30 9 version version NN 10_1101-2020_11_24_390039 30 10 posted post VBD 10_1101-2020_11_24_390039 30 11 January January NNP 10_1101-2020_11_24_390039 30 12 5 5 CD 10_1101-2020_11_24_390039 30 13 , , , 10_1101-2020_11_24_390039 30 14 2021 2021 CD 10_1101-2020_11_24_390039 30 15 . . . 10_1101-2020_11_24_390039 30 16 ; ; : 10_1101-2020_11_24_390039 30 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 30 18 : : : 10_1101-2020_11_24_390039 30 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 30 20 preprint preprint JJ 10_1101-2020_11_24_390039 30 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 30 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 30 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 30 24 - - HYPH 10_1101-2020_11_24_390039 30 25 F F NNP 10_1101-2020_11_24_390039 30 26 as as IN 10_1101-2020_11_24_390039 30 27 a a DT 10_1101-2020_11_24_390039 30 28 potential potential JJ 10_1101-2020_11_24_390039 30 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 30 30 agent agent NN 10_1101-2020_11_24_390039 30 31 Page Page NNP 10_1101-2020_11_24_390039 30 32 4 4 CD 10_1101-2020_11_24_390039 30 33 of of IN 10_1101-2020_11_24_390039 30 34 21 21 CD 10_1101-2020_11_24_390039 30 35 Results Results NNPS 10_1101-2020_11_24_390039 30 36 and and CC 10_1101-2020_11_24_390039 30 37 Discussion Discussion NNP 10_1101-2020_11_24_390039 30 38 Ipom Ipom NNP 10_1101-2020_11_24_390039 30 39 - - HYPH 10_1101-2020_11_24_390039 30 40 F F NNP 10_1101-2020_11_24_390039 30 41 selectively selectively RB 10_1101-2020_11_24_390039 30 42 inhibits inhibit VBZ 10_1101-2020_11_24_390039 30 43 ER ER NNP 10_1101-2020_11_24_390039 30 44 translocation translocation NN 10_1101-2020_11_24_390039 30 45 of of IN 10_1101-2020_11_24_390039 30 46 the the DT 10_1101-2020_11_24_390039 30 47 viral viral JJ 10_1101-2020_11_24_390039 30 48 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 30 49 and and CC 10_1101-2020_11_24_390039 30 50 S S NNP 10_1101-2020_11_24_390039 30 51 proteins protein NNS 10_1101-2020_11_24_390039 30 52 To to TO 10_1101-2020_11_24_390039 30 53 explore explore VB 10_1101-2020_11_24_390039 30 54 the the DT 10_1101-2020_11_24_390039 30 55 ability ability NN 10_1101-2020_11_24_390039 30 56 of of IN 10_1101-2020_11_24_390039 30 57 Ipom Ipom NNP 10_1101-2020_11_24_390039 30 58 - - HYPH 10_1101-2020_11_24_390039 30 59 F F NNP 10_1101-2020_11_24_390039 30 60 to to TO 10_1101-2020_11_24_390039 30 61 inhibit inhibit VB 10_1101-2020_11_24_390039 30 62 the the DT 10_1101-2020_11_24_390039 30 63 ER ER NNP 10_1101-2020_11_24_390039 30 64 translocation translocation NN 10_1101-2020_11_24_390039 30 65 of of IN 10_1101-2020_11_24_390039 30 66 a a DT 10_1101-2020_11_24_390039 30 67 small small JJ 10_1101-2020_11_24_390039 30 68 , , , 10_1101-2020_11_24_390039 30 69 yet yet CC 10_1101-2020_11_24_390039 30 70 structurally structurally RB 10_1101-2020_11_24_390039 30 71 diverse diverse JJ 10_1101-2020_11_24_390039 30 72 , , , 10_1101-2020_11_24_390039 30 73 panel panel NN 10_1101-2020_11_24_390039 30 74 of of IN 10_1101-2020_11_24_390039 30 75 SARS SARS NNP 10_1101-2020_11_24_390039 30 76 - - HYPH 10_1101-2020_11_24_390039 30 77 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 30 78 membrane membrane NN 10_1101-2020_11_24_390039 30 79 and and CC 10_1101-2020_11_24_390039 30 80 secretory secretory JJ 10_1101-2020_11_24_390039 30 81 - - HYPH 10_1101-2020_11_24_390039 30 82 like like JJ 10_1101-2020_11_24_390039 30 83 proteins protein NNS 10_1101-2020_11_24_390039 30 84 , , , 10_1101-2020_11_24_390039 30 85 we -PRON- PRP 10_1101-2020_11_24_390039 30 86 first first RB 10_1101-2020_11_24_390039 30 87 used use VBD 10_1101-2020_11_24_390039 30 88 a a DT 10_1101-2020_11_24_390039 30 89 well well RB 10_1101-2020_11_24_390039 30 90 - - HYPH 10_1101-2020_11_24_390039 30 91 established establish VBN 10_1101-2020_11_24_390039 30 92 in in IN 10_1101-2020_11_24_390039 30 93 vitro vitro FW 10_1101-2020_11_24_390039 30 94 translation translation NN 10_1101-2020_11_24_390039 30 95 system system NN 10_1101-2020_11_24_390039 30 96 supplemented supplement VBN 10_1101-2020_11_24_390039 30 97 with with IN 10_1101-2020_11_24_390039 30 98 canine canine NN 10_1101-2020_11_24_390039 30 99 pancreatic pancreatic JJ 10_1101-2020_11_24_390039 30 100 microsomes microsome NNS 10_1101-2020_11_24_390039 30 101 ( ( -LRB- 10_1101-2020_11_24_390039 30 102 Fig fig NN 10_1101-2020_11_24_390039 30 103 . . . 10_1101-2020_11_24_390039 31 1 2A 2a LS 10_1101-2020_11_24_390039 31 2 ) ) -RRB- 10_1101-2020_11_24_390039 31 3 . . . 10_1101-2020_11_24_390039 32 1 To to TO 10_1101-2020_11_24_390039 32 2 facilitate facilitate VB 10_1101-2020_11_24_390039 32 3 the the DT 10_1101-2020_11_24_390039 32 4 detection detection NN 10_1101-2020_11_24_390039 32 5 of of IN 10_1101-2020_11_24_390039 32 6 ER ER NNP 10_1101-2020_11_24_390039 32 7 translocation translocation NN 10_1101-2020_11_24_390039 32 8 , , , 10_1101-2020_11_24_390039 32 9 we -PRON- PRP 10_1101-2020_11_24_390039 32 10 modified modify VBD 10_1101-2020_11_24_390039 32 11 the the DT 10_1101-2020_11_24_390039 32 12 viral viral NN 10_1101-2020_11_24_390039 32 13 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 32 14 , , , 10_1101-2020_11_24_390039 32 15 S S NNP 10_1101-2020_11_24_390039 32 16 , , , 10_1101-2020_11_24_390039 32 17 E E NNP 10_1101-2020_11_24_390039 32 18 , , , 10_1101-2020_11_24_390039 32 19 M M NNP 10_1101-2020_11_24_390039 32 20 and and CC 10_1101-2020_11_24_390039 32 21 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 32 22 proteins protein VBZ 10_1101-2020_11_24_390039 32 23 by by IN 10_1101-2020_11_24_390039 32 24 adding add VBG 10_1101-2020_11_24_390039 32 25 an an DT 10_1101-2020_11_24_390039 32 26 OPG2-tag OPG2-tag NNP 10_1101-2020_11_24_390039 32 27 ; ; : 10_1101-2020_11_24_390039 32 28 an an DT 10_1101-2020_11_24_390039 32 29 epitope epitope NN 10_1101-2020_11_24_390039 32 30 that that WDT 10_1101-2020_11_24_390039 32 31 supports support VBZ 10_1101-2020_11_24_390039 32 32 efficient efficient JJ 10_1101-2020_11_24_390039 32 33 ER ER NNP 10_1101-2020_11_24_390039 32 34 lumenal lumenal JJ 10_1101-2020_11_24_390039 32 35 N n NN 10_1101-2020_11_24_390039 32 36 - - HYPH 10_1101-2020_11_24_390039 32 37 glycosylation glycosylation NN 10_1101-2020_11_24_390039 32 38 and and CC 10_1101-2020_11_24_390039 32 39 enables enable VBZ 10_1101-2020_11_24_390039 32 40 product product NN 10_1101-2020_11_24_390039 32 41 recovery recovery NN 10_1101-2020_11_24_390039 32 42 via via IN 10_1101-2020_11_24_390039 32 43 immunoprecipitation immunoprecipitation NN 10_1101-2020_11_24_390039 32 44 , , , 10_1101-2020_11_24_390039 32 45 without without IN 10_1101-2020_11_24_390039 32 46 affecting affect VBG 10_1101-2020_11_24_390039 32 47 Ipom Ipom NNP 10_1101-2020_11_24_390039 32 48 - - HYPH 10_1101-2020_11_24_390039 32 49 F F NNP 10_1101-2020_11_24_390039 32 50 sensitivity sensitivity NN 10_1101-2020_11_24_390039 32 51 ( ( -LRB- 10_1101-2020_11_24_390039 32 52 Fig fig NN 10_1101-2020_11_24_390039 32 53 . . . 10_1101-2020_11_24_390039 33 1 S1A s1a LS 10_1101-2020_11_24_390039 33 2 ) ) -RRB- 10_1101-2020_11_24_390039 33 3 ( ( -LRB- 10_1101-2020_11_24_390039 33 4 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 33 5 et et NNP 10_1101-2020_11_24_390039 33 6 al al NNP 10_1101-2020_11_24_390039 33 7 . . NNP 10_1101-2020_11_24_390039 33 8 , , , 10_1101-2020_11_24_390039 33 9 2020 2020 CD 10_1101-2020_11_24_390039 33 10 submitted submit VBN 10_1101-2020_11_24_390039 33 11 ) ) -RRB- 10_1101-2020_11_24_390039 33 12 . . . 10_1101-2020_11_24_390039 34 1 For for IN 10_1101-2020_11_24_390039 34 2 viral viral JJ 10_1101-2020_11_24_390039 34 3 proteins protein NNS 10_1101-2020_11_24_390039 34 4 that that WDT 10_1101-2020_11_24_390039 34 5 lack lack VBP 10_1101-2020_11_24_390039 34 6 endogenous endogenous JJ 10_1101-2020_11_24_390039 34 7 sites site NNS 10_1101-2020_11_24_390039 34 8 for for IN 10_1101-2020_11_24_390039 34 9 N n NN 10_1101-2020_11_24_390039 34 10 - - HYPH 10_1101-2020_11_24_390039 34 11 glycosylation glycosylation NN 10_1101-2020_11_24_390039 34 12 , , , 10_1101-2020_11_24_390039 34 13 such such JJ 10_1101-2020_11_24_390039 34 14 as as IN 10_1101-2020_11_24_390039 34 15 the the DT 10_1101-2020_11_24_390039 34 16 E e NN 10_1101-2020_11_24_390039 34 17 protein protein NN 10_1101-2020_11_24_390039 34 18 , , , 10_1101-2020_11_24_390039 34 19 the the DT 10_1101-2020_11_24_390039 34 20 ER ER NNP 10_1101-2020_11_24_390039 34 21 lumenal lumenal NN 10_1101-2020_11_24_390039 34 22 OPG2-tag opg2-tag NN 10_1101-2020_11_24_390039 34 23 acts act VBZ 10_1101-2020_11_24_390039 34 24 as as IN 10_1101-2020_11_24_390039 34 25 a a DT 10_1101-2020_11_24_390039 34 26 reporter reporter NN 10_1101-2020_11_24_390039 34 27 for for IN 10_1101-2020_11_24_390039 34 28 ER ER NNP 10_1101-2020_11_24_390039 34 29 translocation translocation NN 10_1101-2020_11_24_390039 34 30 and and CC 10_1101-2020_11_24_390039 34 31 enables enable VBZ 10_1101-2020_11_24_390039 34 32 their -PRON- PRP$ 10_1101-2020_11_24_390039 34 33 recovery recovery NN 10_1101-2020_11_24_390039 34 34 of of IN 10_1101-2020_11_24_390039 34 35 by by IN 10_1101-2020_11_24_390039 34 36 immunoprecipitation immunoprecipitation NN 10_1101-2020_11_24_390039 34 37 . . . 10_1101-2020_11_24_390039 35 1 Where where WRB 10_1101-2020_11_24_390039 35 2 viral viral JJ 10_1101-2020_11_24_390039 35 3 proteins protein NNS 10_1101-2020_11_24_390039 35 4 already already RB 10_1101-2020_11_24_390039 35 5 contain contain VBP 10_1101-2020_11_24_390039 35 6 suitable suitable JJ 10_1101-2020_11_24_390039 35 7 sites site NNS 10_1101-2020_11_24_390039 35 8 for for IN 10_1101-2020_11_24_390039 35 9 N- n- CD 10_1101-2020_11_24_390039 35 10 glycosylation glycosylation NN 10_1101-2020_11_24_390039 35 11 ( ( -LRB- 10_1101-2020_11_24_390039 35 12 S S NNP 10_1101-2020_11_24_390039 35 13 and and CC 10_1101-2020_11_24_390039 35 14 M M NNP 10_1101-2020_11_24_390039 35 15 proteins protein NNS 10_1101-2020_11_24_390039 35 16 ) ) -RRB- 10_1101-2020_11_24_390039 35 17 , , , 10_1101-2020_11_24_390039 35 18 the the DT 10_1101-2020_11_24_390039 35 19 cytosolic cytosolic NN 10_1101-2020_11_24_390039 35 20 OPG2-tag OPG2-tag NNP 10_1101-2020_11_24_390039 35 21 is be VBZ 10_1101-2020_11_24_390039 35 22 used use VBN 10_1101-2020_11_24_390039 35 23 solely solely RB 10_1101-2020_11_24_390039 35 24 for for IN 10_1101-2020_11_24_390039 35 25 immunoprecipitation immunoprecipitation NN 10_1101-2020_11_24_390039 35 26 . . . 10_1101-2020_11_24_390039 36 1 The the DT 10_1101-2020_11_24_390039 36 2 identity identity NN 10_1101-2020_11_24_390039 36 3 of of IN 10_1101-2020_11_24_390039 36 4 the the DT 10_1101-2020_11_24_390039 36 5 resulting result VBG 10_1101-2020_11_24_390039 36 6 N n CD 10_1101-2020_11_24_390039 36 7 - - HYPH 10_1101-2020_11_24_390039 36 8 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 36 9 species specie NNS 10_1101-2020_11_24_390039 36 10 for for IN 10_1101-2020_11_24_390039 36 11 each each DT 10_1101-2020_11_24_390039 36 12 of of IN 10_1101-2020_11_24_390039 36 13 these these DT 10_1101-2020_11_24_390039 36 14 OPG2-tagged opg2-tagged JJ 10_1101-2020_11_24_390039 36 15 viral viral JJ 10_1101-2020_11_24_390039 36 16 proteins protein NNS 10_1101-2020_11_24_390039 36 17 was be VBD 10_1101-2020_11_24_390039 36 18 confirmed confirm VBN 10_1101-2020_11_24_390039 36 19 by by IN 10_1101-2020_11_24_390039 36 20 endoglycosidase endoglycosidase NN 10_1101-2020_11_24_390039 36 21 H h NN 10_1101-2020_11_24_390039 36 22 ( ( -LRB- 10_1101-2020_11_24_390039 36 23 Endo Endo NNP 10_1101-2020_11_24_390039 36 24 H h NN 10_1101-2020_11_24_390039 36 25 ) ) -RRB- 10_1101-2020_11_24_390039 36 26 treatment treatment NN 10_1101-2020_11_24_390039 36 27 of of IN 10_1101-2020_11_24_390039 36 28 the the DT 10_1101-2020_11_24_390039 36 29 radiolabelled radiolabelle VBN 10_1101-2020_11_24_390039 36 30 products product NNS 10_1101-2020_11_24_390039 36 31 associated associate VBN 10_1101-2020_11_24_390039 36 32 with with IN 10_1101-2020_11_24_390039 36 33 the the DT 10_1101-2020_11_24_390039 36 34 membrane membrane NN 10_1101-2020_11_24_390039 36 35 fraction fraction NN 10_1101-2020_11_24_390039 36 36 prior prior RB 10_1101-2020_11_24_390039 36 37 to to IN 10_1101-2020_11_24_390039 36 38 SDS SDS NNP 10_1101-2020_11_24_390039 36 39 - - HYPH 10_1101-2020_11_24_390039 36 40 PAGE PAGE NNP 10_1101-2020_11_24_390039 36 41 ( ( -LRB- 10_1101-2020_11_24_390039 36 42 Fig fig NN 10_1101-2020_11_24_390039 36 43 . . . 10_1101-2020_11_24_390039 37 1 2B 2B NNP 10_1101-2020_11_24_390039 37 2 , , , 10_1101-2020_11_24_390039 37 3 cf cf NNP 10_1101-2020_11_24_390039 37 4 . . . 10_1101-2020_11_24_390039 38 1 lanes lanes NNP 10_1101-2020_11_24_390039 38 2 1 1 CD 10_1101-2020_11_24_390039 38 3 and and CC 10_1101-2020_11_24_390039 38 4 2 2 CD 10_1101-2020_11_24_390039 38 5 in in IN 10_1101-2020_11_24_390039 38 6 each each DT 10_1101-2020_11_24_390039 38 7 panel panel NN 10_1101-2020_11_24_390039 38 8 ) ) -RRB- 10_1101-2020_11_24_390039 38 9 . . . 10_1101-2020_11_24_390039 39 1 Using use VBG 10_1101-2020_11_24_390039 39 2 ER ER NNP 10_1101-2020_11_24_390039 39 3 lumenal lumenal JJ 10_1101-2020_11_24_390039 39 4 modification modification NN 10_1101-2020_11_24_390039 39 5 of of IN 10_1101-2020_11_24_390039 39 6 either either DT 10_1101-2020_11_24_390039 39 7 endogenous endogenous JJ 10_1101-2020_11_24_390039 39 8 N N NNP 10_1101-2020_11_24_390039 39 9 - - HYPH 10_1101-2020_11_24_390039 39 10 glycosylation glycosylation NN 10_1101-2020_11_24_390039 39 11 sites site NNS 10_1101-2020_11_24_390039 39 12 ( ( -LRB- 10_1101-2020_11_24_390039 39 13 viral viral JJ 10_1101-2020_11_24_390039 39 14 S S NNP 10_1101-2020_11_24_390039 39 15 and and CC 10_1101-2020_11_24_390039 39 16 M M NNP 10_1101-2020_11_24_390039 39 17 proteins protein NNS 10_1101-2020_11_24_390039 39 18 ) ) -RRB- 10_1101-2020_11_24_390039 39 19 or or CC 10_1101-2020_11_24_390039 39 20 the the DT 10_1101-2020_11_24_390039 39 21 appended append VBN 10_1101-2020_11_24_390039 39 22 OPG2-tag opg2-tag NN 10_1101-2020_11_24_390039 39 23 ( ( -LRB- 10_1101-2020_11_24_390039 39 24 viral viral JJ 10_1101-2020_11_24_390039 39 25 E e NN 10_1101-2020_11_24_390039 39 26 and and CC 10_1101-2020_11_24_390039 39 27 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 39 28 proteins protein NNS 10_1101-2020_11_24_390039 39 29 ) ) -RRB- 10_1101-2020_11_24_390039 39 30 as as IN 10_1101-2020_11_24_390039 39 31 a a DT 10_1101-2020_11_24_390039 39 32 reporter reporter NN 10_1101-2020_11_24_390039 39 33 for for IN 10_1101-2020_11_24_390039 39 34 ER ER NNP 10_1101-2020_11_24_390039 39 35 membrane membrane NN 10_1101-2020_11_24_390039 39 36 translocation translocation NN 10_1101-2020_11_24_390039 39 37 , , , 10_1101-2020_11_24_390039 39 38 we -PRON- PRP 10_1101-2020_11_24_390039 39 39 found find VBD 10_1101-2020_11_24_390039 39 40 that that IN 10_1101-2020_11_24_390039 39 41 1 1 CD 10_1101-2020_11_24_390039 39 42 µM µM NNP 10_1101-2020_11_24_390039 39 43 Ipom Ipom NNP 10_1101-2020_11_24_390039 39 44 - - HYPH 10_1101-2020_11_24_390039 39 45 F F NNP 10_1101-2020_11_24_390039 39 46 strongly strongly RB 10_1101-2020_11_24_390039 39 47 inhibited inhibit VBD 10_1101-2020_11_24_390039 39 48 both both CC 10_1101-2020_11_24_390039 39 49 the the DT 10_1101-2020_11_24_390039 39 50 translocation translocation NN 10_1101-2020_11_24_390039 39 51 of of IN 10_1101-2020_11_24_390039 39 52 the the DT 10_1101-2020_11_24_390039 39 53 soluble soluble JJ 10_1101-2020_11_24_390039 39 54 , , , 10_1101-2020_11_24_390039 39 55 secretory secretory JJ 10_1101-2020_11_24_390039 39 56 - - HYPH 10_1101-2020_11_24_390039 39 57 like like JJ 10_1101-2020_11_24_390039 39 58 protein protein NN 10_1101-2020_11_24_390039 39 59 ORF8-OPG2 ORF8-OPG2 NNP 10_1101-2020_11_24_390039 39 60 and and CC 10_1101-2020_11_24_390039 39 61 the the DT 10_1101-2020_11_24_390039 39 62 integration integration NN 10_1101-2020_11_24_390039 39 63 of of IN 10_1101-2020_11_24_390039 39 64 the the DT 10_1101-2020_11_24_390039 39 65 type type NN 10_1101-2020_11_24_390039 39 66 I -PRON- PRP 10_1101-2020_11_24_390039 39 67 transmembrane transmembrane VBP 10_1101-2020_11_24_390039 39 68 proteins protein NNS 10_1101-2020_11_24_390039 39 69 ( ( -LRB- 10_1101-2020_11_24_390039 39 70 TMP TMP NNP 10_1101-2020_11_24_390039 39 71 ) ) -RRB- 10_1101-2020_11_24_390039 39 72 S S NNP 10_1101-2020_11_24_390039 39 73 - - HYPH 10_1101-2020_11_24_390039 39 74 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 39 75 , , , 10_1101-2020_11_24_390039 39 76 and and CC 10_1101-2020_11_24_390039 39 77 truncated truncate VBN 10_1101-2020_11_24_390039 39 78 derivatives derivative NNS 10_1101-2020_11_24_390039 39 79 thereof thereof RB 10_1101-2020_11_24_390039 39 80 ( ( -LRB- 10_1101-2020_11_24_390039 39 81 Fig fig NN 10_1101-2020_11_24_390039 39 82 . . . 10_1101-2020_11_24_390039 40 1 2B 2B NNP 10_1101-2020_11_24_390039 40 2 , , , 10_1101-2020_11_24_390039 40 3 Fig Fig NNP 10_1101-2020_11_24_390039 40 4 . . . 10_1101-2020_11_24_390039 41 1 2C 2c LS 10_1101-2020_11_24_390039 41 2 , , , 10_1101-2020_11_24_390039 41 3 Fig Fig NNP 10_1101-2020_11_24_390039 41 4 . . . 10_1101-2020_11_24_390039 42 1 S1C s1c LS 10_1101-2020_11_24_390039 42 2 ) ) -RRB- 10_1101-2020_11_24_390039 42 3 . . . 10_1101-2020_11_24_390039 43 1 Furthermore furthermore RB 10_1101-2020_11_24_390039 43 2 , , , 10_1101-2020_11_24_390039 43 3 membrane membrane NN 10_1101-2020_11_24_390039 43 4 insertion insertion NN 10_1101-2020_11_24_390039 43 5 of of IN 10_1101-2020_11_24_390039 43 6 the the DT 10_1101-2020_11_24_390039 43 7 human human JJ 10_1101-2020_11_24_390039 43 8 type type NN 10_1101-2020_11_24_390039 43 9 I -PRON- PRP 10_1101-2020_11_24_390039 43 10 TMP TMP NNP 10_1101-2020_11_24_390039 43 11 , , , 10_1101-2020_11_24_390039 43 12 ACE2 ACE2 NNP 10_1101-2020_11_24_390039 43 13 , , , 10_1101-2020_11_24_390039 43 14 was be VBD 10_1101-2020_11_24_390039 43 15 inhibited inhibit VBN 10_1101-2020_11_24_390039 43 16 to to IN 10_1101-2020_11_24_390039 43 17 a a DT 10_1101-2020_11_24_390039 43 18 similar similar JJ 10_1101-2020_11_24_390039 43 19 extent extent NN 10_1101-2020_11_24_390039 43 20 ( ( -LRB- 10_1101-2020_11_24_390039 43 21 Fig fig NN 10_1101-2020_11_24_390039 43 22 . . . 10_1101-2020_11_24_390039 44 1 2B 2B NNP 10_1101-2020_11_24_390039 44 2 , , , 10_1101-2020_11_24_390039 44 3 Fig Fig NNP 10_1101-2020_11_24_390039 44 4 . . . 10_1101-2020_11_24_390039 45 1 2C 2C NNP 10_1101-2020_11_24_390039 45 2 , , , 10_1101-2020_11_24_390039 45 3 ~70 ~70 : 10_1101-2020_11_24_390039 45 4 to to IN 10_1101-2020_11_24_390039 45 5 ~90 ~90 $ 10_1101-2020_11_24_390039 45 6 % % NN 10_1101-2020_11_24_390039 45 7 inhibition inhibition NN 10_1101-2020_11_24_390039 45 8 for for IN 10_1101-2020_11_24_390039 45 9 these these DT 10_1101-2020_11_24_390039 45 10 three three CD 10_1101-2020_11_24_390039 45 11 proteins protein NNS 10_1101-2020_11_24_390039 45 12 ) ) -RRB- 10_1101-2020_11_24_390039 45 13 . . . 10_1101-2020_11_24_390039 46 1 These these DT 10_1101-2020_11_24_390039 46 2 results result NNS 10_1101-2020_11_24_390039 46 3 mirror mirror VBP 10_1101-2020_11_24_390039 46 4 previous previous JJ 10_1101-2020_11_24_390039 46 5 findings finding NNS 10_1101-2020_11_24_390039 46 6 showing show VBG 10_1101-2020_11_24_390039 46 7 that that IN 10_1101-2020_11_24_390039 46 8 precursor precursor NN 10_1101-2020_11_24_390039 46 9 proteins protein NNS 10_1101-2020_11_24_390039 46 10 bearing bearing NN 10_1101-2020_11_24_390039 46 11 N- n- CD 10_1101-2020_11_24_390039 46 12 terminal terminal JJ 10_1101-2020_11_24_390039 46 13 signal signal NN 10_1101-2020_11_24_390039 46 14 peptides peptide NNS 10_1101-2020_11_24_390039 46 15 , , , 10_1101-2020_11_24_390039 46 16 and and CC 10_1101-2020_11_24_390039 46 17 which which WDT 10_1101-2020_11_24_390039 46 18 are be VBP 10_1101-2020_11_24_390039 46 19 therefore therefore RB 10_1101-2020_11_24_390039 46 20 obligate obligate NN 10_1101-2020_11_24_390039 46 21 clients client NNS 10_1101-2020_11_24_390039 46 22 for for IN 10_1101-2020_11_24_390039 46 23 the the DT 10_1101-2020_11_24_390039 46 24 Sec61- sec61- XX 10_1101-2020_11_24_390039 46 25 translocon translocon NNP 10_1101-2020_11_24_390039 46 26 , , , 10_1101-2020_11_24_390039 46 27 are be VBP 10_1101-2020_11_24_390039 46 28 typically typically RB 10_1101-2020_11_24_390039 46 29 very very RB 10_1101-2020_11_24_390039 46 30 sensitive sensitive JJ 10_1101-2020_11_24_390039 46 31 to to IN 10_1101-2020_11_24_390039 46 32 Ipom Ipom NNP 10_1101-2020_11_24_390039 46 33 - - HYPH 10_1101-2020_11_24_390039 46 34 F F NNP 10_1101-2020_11_24_390039 46 35 - - HYPH 10_1101-2020_11_24_390039 46 36 mediated mediate VBN 10_1101-2020_11_24_390039 46 37 inhibition inhibition NN 10_1101-2020_11_24_390039 46 38 ( ( -LRB- 10_1101-2020_11_24_390039 46 39 Zong Zong NNP 10_1101-2020_11_24_390039 46 40 et et NNP 10_1101-2020_11_24_390039 46 41 al al NNP 10_1101-2020_11_24_390039 46 42 . . NNP 10_1101-2020_11_24_390039 46 43 , , , 10_1101-2020_11_24_390039 46 44 2019 2019 CD 10_1101-2020_11_24_390039 46 45 ; ; : 10_1101-2020_11_24_390039 46 46 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 46 47 et et NNP 10_1101-2020_11_24_390039 46 48 al al NNP 10_1101-2020_11_24_390039 46 49 . . NNP 10_1101-2020_11_24_390039 46 50 , , , 10_1101-2020_11_24_390039 46 51 2020 2020 CD 10_1101-2020_11_24_390039 46 52 submitted submit VBN 10_1101-2020_11_24_390039 46 53 ) ) -RRB- 10_1101-2020_11_24_390039 46 54 . . . 10_1101-2020_11_24_390039 47 1 In in IN 10_1101-2020_11_24_390039 47 2 the the DT 10_1101-2020_11_24_390039 47 3 context context NN 10_1101-2020_11_24_390039 47 4 of of IN 10_1101-2020_11_24_390039 47 5 SARS SARS NNP 10_1101-2020_11_24_390039 47 6 - - HYPH 10_1101-2020_11_24_390039 47 7 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 47 8 infection infection NN 10_1101-2020_11_24_390039 47 9 , , , 10_1101-2020_11_24_390039 47 10 wherein wherein WRB 10_1101-2020_11_24_390039 47 11 ACE2 ACE2 NNP 10_1101-2020_11_24_390039 47 12 acts act VBZ 10_1101-2020_11_24_390039 47 13 as as IN 10_1101-2020_11_24_390039 47 14 an an DT 10_1101-2020_11_24_390039 47 15 important important JJ 10_1101-2020_11_24_390039 47 16 host host NN 10_1101-2020_11_24_390039 47 17 cell cell NN 10_1101-2020_11_24_390039 47 18 receptor receptor NN 10_1101-2020_11_24_390039 47 19 for for IN 10_1101-2020_11_24_390039 47 20 the the DT 10_1101-2020_11_24_390039 47 21 SARS SARS NNP 10_1101-2020_11_24_390039 47 22 - - HYPH 10_1101-2020_11_24_390039 47 23 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 47 24 virus virus NN 10_1101-2020_11_24_390039 47 25 .CC .CC , 10_1101-2020_11_24_390039 47 26 - - : 10_1101-2020_11_24_390039 47 27 BY by IN 10_1101-2020_11_24_390039 47 28 - - HYPH 10_1101-2020_11_24_390039 47 29 ND ND NNP 10_1101-2020_11_24_390039 47 30 4.0 4.0 CD 10_1101-2020_11_24_390039 47 31 International international JJ 10_1101-2020_11_24_390039 47 32 licensemade licensemade NN 10_1101-2020_11_24_390039 47 33 available available JJ 10_1101-2020_11_24_390039 47 34 under under IN 10_1101-2020_11_24_390039 47 35 a a DT 10_1101-2020_11_24_390039 47 36 ( ( -LRB- 10_1101-2020_11_24_390039 47 37 which which WDT 10_1101-2020_11_24_390039 47 38 was be VBD 10_1101-2020_11_24_390039 47 39 not not RB 10_1101-2020_11_24_390039 47 40 certified certify VBN 10_1101-2020_11_24_390039 47 41 by by IN 10_1101-2020_11_24_390039 47 42 peer peer NN 10_1101-2020_11_24_390039 47 43 review review NN 10_1101-2020_11_24_390039 47 44 ) ) -RRB- 10_1101-2020_11_24_390039 47 45 is be VBZ 10_1101-2020_11_24_390039 47 46 the the DT 10_1101-2020_11_24_390039 47 47 author author NN 10_1101-2020_11_24_390039 47 48 / / SYM 10_1101-2020_11_24_390039 47 49 funder funder NN 10_1101-2020_11_24_390039 47 50 , , , 10_1101-2020_11_24_390039 47 51 who who WP 10_1101-2020_11_24_390039 47 52 has have VBZ 10_1101-2020_11_24_390039 47 53 granted grant VBN 10_1101-2020_11_24_390039 47 54 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 47 55 a a DT 10_1101-2020_11_24_390039 47 56 license license NN 10_1101-2020_11_24_390039 47 57 to to TO 10_1101-2020_11_24_390039 47 58 display display VB 10_1101-2020_11_24_390039 47 59 the the DT 10_1101-2020_11_24_390039 47 60 preprint preprint NN 10_1101-2020_11_24_390039 47 61 in in IN 10_1101-2020_11_24_390039 47 62 perpetuity perpetuity NN 10_1101-2020_11_24_390039 47 63 . . . 10_1101-2020_11_24_390039 48 1 It -PRON- PRP 10_1101-2020_11_24_390039 48 2 is be VBZ 10_1101-2020_11_24_390039 48 3 The the DT 10_1101-2020_11_24_390039 48 4 copyright copyright NN 10_1101-2020_11_24_390039 48 5 holder holder NN 10_1101-2020_11_24_390039 48 6 for for IN 10_1101-2020_11_24_390039 48 7 this this DT 10_1101-2020_11_24_390039 48 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 48 9 version version NN 10_1101-2020_11_24_390039 48 10 posted post VBD 10_1101-2020_11_24_390039 48 11 January January NNP 10_1101-2020_11_24_390039 48 12 5 5 CD 10_1101-2020_11_24_390039 48 13 , , , 10_1101-2020_11_24_390039 48 14 2021 2021 CD 10_1101-2020_11_24_390039 48 15 . . . 10_1101-2020_11_24_390039 48 16 ; ; : 10_1101-2020_11_24_390039 48 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 48 18 : : : 10_1101-2020_11_24_390039 48 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 48 20 preprint preprint JJ 10_1101-2020_11_24_390039 48 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 48 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 48 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 48 24 - - HYPH 10_1101-2020_11_24_390039 48 25 F F NNP 10_1101-2020_11_24_390039 48 26 as as IN 10_1101-2020_11_24_390039 48 27 a a DT 10_1101-2020_11_24_390039 48 28 potential potential JJ 10_1101-2020_11_24_390039 48 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 48 30 agent agent NN 10_1101-2020_11_24_390039 48 31 Page Page NNP 10_1101-2020_11_24_390039 48 32 5 5 CD 10_1101-2020_11_24_390039 48 33 of of IN 10_1101-2020_11_24_390039 48 34 21 21 CD 10_1101-2020_11_24_390039 48 35 via via IN 10_1101-2020_11_24_390039 48 36 its -PRON- PRP$ 10_1101-2020_11_24_390039 48 37 interaction interaction NN 10_1101-2020_11_24_390039 48 38 with with IN 10_1101-2020_11_24_390039 48 39 the the DT 10_1101-2020_11_24_390039 48 40 viral viral JJ 10_1101-2020_11_24_390039 48 41 S s JJ 10_1101-2020_11_24_390039 48 42 protein protein NN 10_1101-2020_11_24_390039 48 43 ( ( -LRB- 10_1101-2020_11_24_390039 48 44 Walls Walls NNP 10_1101-2020_11_24_390039 48 45 et et FW 10_1101-2020_11_24_390039 48 46 al al NNP 10_1101-2020_11_24_390039 48 47 . . NNP 10_1101-2020_11_24_390039 48 48 , , , 10_1101-2020_11_24_390039 48 49 2020 2020 CD 10_1101-2020_11_24_390039 48 50 ) ) -RRB- 10_1101-2020_11_24_390039 48 51 , , , 10_1101-2020_11_24_390039 48 52 these these DT 10_1101-2020_11_24_390039 48 53 data datum NNS 10_1101-2020_11_24_390039 48 54 suggest suggest VBP 10_1101-2020_11_24_390039 48 55 that that IN 10_1101-2020_11_24_390039 48 56 an an DT 10_1101-2020_11_24_390039 48 57 Ipom Ipom NNP 10_1101-2020_11_24_390039 48 58 - - HYPH 10_1101-2020_11_24_390039 48 59 F F NNP 10_1101-2020_11_24_390039 48 60 - - HYPH 10_1101-2020_11_24_390039 48 61 induced induce VBN 10_1101-2020_11_24_390039 48 62 antiviral antiviral JJ 10_1101-2020_11_24_390039 48 63 effect effect NN 10_1101-2020_11_24_390039 48 64 might may MD 10_1101-2020_11_24_390039 48 65 be be VB 10_1101-2020_11_24_390039 48 66 achieved achieve VBN 10_1101-2020_11_24_390039 48 67 via via IN 10_1101-2020_11_24_390039 48 68 selective selective JJ 10_1101-2020_11_24_390039 48 69 reductions reduction NNS 10_1101-2020_11_24_390039 48 70 in in IN 10_1101-2020_11_24_390039 48 71 the the DT 10_1101-2020_11_24_390039 48 72 biogenesis biogenesis NN 10_1101-2020_11_24_390039 48 73 of of IN 10_1101-2020_11_24_390039 48 74 both both DT 10_1101-2020_11_24_390039 48 75 host host NN 10_1101-2020_11_24_390039 48 76 and and CC 10_1101-2020_11_24_390039 48 77 viral viral JJ 10_1101-2020_11_24_390039 48 78 proteins protein NNS 10_1101-2020_11_24_390039 48 79 ( ( -LRB- 10_1101-2020_11_24_390039 48 80 cf cf NN 10_1101-2020_11_24_390039 48 81 . . . 10_1101-2020_11_24_390039 49 1 Fig fig NN 10_1101-2020_11_24_390039 49 2 . . . 10_1101-2020_11_24_390039 50 1 1A 1a LS 10_1101-2020_11_24_390039 50 2 ) ) -RRB- 10_1101-2020_11_24_390039 50 3 . . . 10_1101-2020_11_24_390039 51 1 In in IN 10_1101-2020_11_24_390039 51 2 contrast contrast NN 10_1101-2020_11_24_390039 51 3 to to IN 10_1101-2020_11_24_390039 51 4 the the DT 10_1101-2020_11_24_390039 51 5 viral viral JJ 10_1101-2020_11_24_390039 51 6 S S NNP 10_1101-2020_11_24_390039 51 7 and and CC 10_1101-2020_11_24_390039 51 8 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 51 9 proteins protein NNS 10_1101-2020_11_24_390039 51 10 , , , 10_1101-2020_11_24_390039 51 11 insertion insertion NN 10_1101-2020_11_24_390039 51 12 of of IN 10_1101-2020_11_24_390039 51 13 the the DT 10_1101-2020_11_24_390039 51 14 viral viral JJ 10_1101-2020_11_24_390039 51 15 E e NN 10_1101-2020_11_24_390039 51 16 protein protein NN 10_1101-2020_11_24_390039 51 17 was be VBD 10_1101-2020_11_24_390039 51 18 unaffected unaffected JJ 10_1101-2020_11_24_390039 51 19 by by IN 10_1101-2020_11_24_390039 51 20 Ipom Ipom NNP 10_1101-2020_11_24_390039 51 21 - - HYPH 10_1101-2020_11_24_390039 51 22 F F NNP 10_1101-2020_11_24_390039 51 23 ( ( -LRB- 10_1101-2020_11_24_390039 51 24 Fig Fig NNP 10_1101-2020_11_24_390039 51 25 . . . 10_1101-2020_11_24_390039 52 1 2B 2B NNP 10_1101-2020_11_24_390039 52 2 - - HYPH 10_1101-2020_11_24_390039 52 3 C C NNP 10_1101-2020_11_24_390039 52 4 ) ) -RRB- 10_1101-2020_11_24_390039 52 5 , , , 10_1101-2020_11_24_390039 52 6 consistent consistent JJ 10_1101-2020_11_24_390039 52 7 with with IN 10_1101-2020_11_24_390039 52 8 its -PRON- PRP$ 10_1101-2020_11_24_390039 52 9 recent recent JJ 10_1101-2020_11_24_390039 52 10 classification classification NN 10_1101-2020_11_24_390039 52 11 as as IN 10_1101-2020_11_24_390039 52 12 a a DT 10_1101-2020_11_24_390039 52 13 type type NN 10_1101-2020_11_24_390039 52 14 III iii CD 10_1101-2020_11_24_390039 52 15 TMP TMP NNP 10_1101-2020_11_24_390039 52 16 ( ( -LRB- 10_1101-2020_11_24_390039 52 17 Duart Duart NNP 10_1101-2020_11_24_390039 52 18 et et NNP 10_1101-2020_11_24_390039 52 19 al al NNP 10_1101-2020_11_24_390039 52 20 . . NNP 10_1101-2020_11_24_390039 52 21 , , , 10_1101-2020_11_24_390039 52 22 2020 2020 CD 10_1101-2020_11_24_390039 52 23 ) ) -RRB- 10_1101-2020_11_24_390039 52 24 . . . 10_1101-2020_11_24_390039 53 1 Type type NN 10_1101-2020_11_24_390039 53 2 III III NNP 10_1101-2020_11_24_390039 53 3 TMP tmp NN 10_1101-2020_11_24_390039 53 4 integration integration NN 10_1101-2020_11_24_390039 53 5 is be VBZ 10_1101-2020_11_24_390039 53 6 highly highly RB 10_1101-2020_11_24_390039 53 7 resistant resistant JJ 10_1101-2020_11_24_390039 53 8 to to IN 10_1101-2020_11_24_390039 53 9 Ipom Ipom NNP 10_1101-2020_11_24_390039 53 10 - - HYPH 10_1101-2020_11_24_390039 53 11 F F NNP 10_1101-2020_11_24_390039 53 12 ( ( -LRB- 10_1101-2020_11_24_390039 53 13 Zong Zong NNP 10_1101-2020_11_24_390039 53 14 et et NNP 10_1101-2020_11_24_390039 53 15 al al NNP 10_1101-2020_11_24_390039 53 16 . . NNP 10_1101-2020_11_24_390039 53 17 , , , 10_1101-2020_11_24_390039 53 18 2019 2019 CD 10_1101-2020_11_24_390039 53 19 ) ) -RRB- 10_1101-2020_11_24_390039 53 20 , , , 10_1101-2020_11_24_390039 53 21 most most RBS 10_1101-2020_11_24_390039 53 22 likely likely RB 10_1101-2020_11_24_390039 53 23 because because IN 10_1101-2020_11_24_390039 53 24 they -PRON- PRP 10_1101-2020_11_24_390039 53 25 exploit exploit VBP 10_1101-2020_11_24_390039 53 26 a a DT 10_1101-2020_11_24_390039 53 27 novel novel JJ 10_1101-2020_11_24_390039 53 28 pathway pathway NN 10_1101-2020_11_24_390039 53 29 for for IN 10_1101-2020_11_24_390039 53 30 ER ER NNP 10_1101-2020_11_24_390039 53 31 insertion insertion NN 10_1101-2020_11_24_390039 53 32 ( ( -LRB- 10_1101-2020_11_24_390039 53 33 cf cf NN 10_1101-2020_11_24_390039 53 34 . . . 10_1101-2020_11_24_390039 54 1 Fig fig NN 10_1101-2020_11_24_390039 54 2 . . . 10_1101-2020_11_24_390039 55 1 3 3 LS 10_1101-2020_11_24_390039 55 2 ; ; : 10_1101-2020_11_24_390039 55 3 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 55 4 et et NNP 10_1101-2020_11_24_390039 55 5 al al NNP 10_1101-2020_11_24_390039 55 6 . . NNP 10_1101-2020_11_24_390039 55 7 , , , 10_1101-2020_11_24_390039 55 8 2020 2020 CD 10_1101-2020_11_24_390039 55 9 submitted submit VBN 10_1101-2020_11_24_390039 55 10 ) ) -RRB- 10_1101-2020_11_24_390039 55 11 . . . 10_1101-2020_11_24_390039 56 1 We -PRON- PRP 10_1101-2020_11_24_390039 56 2 therefore therefore RB 10_1101-2020_11_24_390039 56 3 conclude conclude VBP 10_1101-2020_11_24_390039 56 4 that that IN 10_1101-2020_11_24_390039 56 5 the the DT 10_1101-2020_11_24_390039 56 6 known know VBN 10_1101-2020_11_24_390039 56 7 substrate substrate NN 10_1101-2020_11_24_390039 56 8 - - HYPH 10_1101-2020_11_24_390039 56 9 selective selective JJ 10_1101-2020_11_24_390039 56 10 inhibitory inhibitory JJ 10_1101-2020_11_24_390039 56 11 action action NN 10_1101-2020_11_24_390039 56 12 of of IN 10_1101-2020_11_24_390039 56 13 Ipom Ipom NNP 10_1101-2020_11_24_390039 56 14 - - HYPH 10_1101-2020_11_24_390039 56 15 F F NNP 10_1101-2020_11_24_390039 56 16 at at IN 10_1101-2020_11_24_390039 56 17 the the DT 10_1101-2020_11_24_390039 56 18 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 56 19 translocon translocon NN 10_1101-2020_11_24_390039 56 20 is be VBZ 10_1101-2020_11_24_390039 56 21 directly directly RB 10_1101-2020_11_24_390039 56 22 applicable applicable JJ 10_1101-2020_11_24_390039 56 23 to to IN 10_1101-2020_11_24_390039 56 24 viral viral JJ 10_1101-2020_11_24_390039 56 25 membrane membrane NN 10_1101-2020_11_24_390039 56 26 proteins protein NNS 10_1101-2020_11_24_390039 56 27 ; ; , 10_1101-2020_11_24_390039 56 28 whereby whereby WRB 10_1101-2020_11_24_390039 56 29 the the DT 10_1101-2020_11_24_390039 56 30 ER ER NNP 10_1101-2020_11_24_390039 56 31 translocation translocation NN 10_1101-2020_11_24_390039 56 32 of of IN 10_1101-2020_11_24_390039 56 33 secretory secretory JJ 10_1101-2020_11_24_390039 56 34 proteins protein NNS 10_1101-2020_11_24_390039 56 35 and and CC 10_1101-2020_11_24_390039 56 36 type type NN 10_1101-2020_11_24_390039 56 37 I -PRON- PRP 10_1101-2020_11_24_390039 56 38 TMPs tmp NNS 10_1101-2020_11_24_390039 56 39 , , , 10_1101-2020_11_24_390039 56 40 but but CC 10_1101-2020_11_24_390039 56 41 not not RB 10_1101-2020_11_24_390039 56 42 type type VB 10_1101-2020_11_24_390039 56 43 III iii CD 10_1101-2020_11_24_390039 56 44 TMPs tmp NNS 10_1101-2020_11_24_390039 56 45 , , , 10_1101-2020_11_24_390039 56 46 is be VBZ 10_1101-2020_11_24_390039 56 47 efficiently efficiently RB 10_1101-2020_11_24_390039 56 48 blocked block VBN 10_1101-2020_11_24_390039 56 49 by by IN 10_1101-2020_11_24_390039 56 50 Ipom Ipom NNP 10_1101-2020_11_24_390039 56 51 - - HYPH 10_1101-2020_11_24_390039 56 52 F. F. NNP 10_1101-2020_11_24_390039 57 1 The the DT 10_1101-2020_11_24_390039 57 2 viral viral JJ 10_1101-2020_11_24_390039 57 3 M M NNP 10_1101-2020_11_24_390039 57 4 protein protein NN 10_1101-2020_11_24_390039 57 5 is be VBZ 10_1101-2020_11_24_390039 57 6 a a DT 10_1101-2020_11_24_390039 57 7 multi multi JJ 10_1101-2020_11_24_390039 57 8 - - JJ 10_1101-2020_11_24_390039 57 9 pass pass JJ 10_1101-2020_11_24_390039 57 10 TMP tmp NN 10_1101-2020_11_24_390039 57 11 with with IN 10_1101-2020_11_24_390039 57 12 its -PRON- PRP$ 10_1101-2020_11_24_390039 57 13 first first JJ 10_1101-2020_11_24_390039 57 14 TMD TMD NNP 10_1101-2020_11_24_390039 57 15 oriented orient VBN 10_1101-2020_11_24_390039 57 16 so so IN 10_1101-2020_11_24_390039 57 17 the the DT 10_1101-2020_11_24_390039 57 18 N- n- CD 10_1101-2020_11_24_390039 57 19 terminus terminus NN 10_1101-2020_11_24_390039 57 20 is be VBZ 10_1101-2020_11_24_390039 57 21 exoplasmic exoplasmic JJ 10_1101-2020_11_24_390039 57 22 ( ( -LRB- 10_1101-2020_11_24_390039 57 23 Nexo Nexo NNP 10_1101-2020_11_24_390039 57 24 ) ) -RRB- 10_1101-2020_11_24_390039 57 25 and and CC 10_1101-2020_11_24_390039 57 26 hence hence RB 10_1101-2020_11_24_390039 57 27 can can MD 10_1101-2020_11_24_390039 57 28 be be VB 10_1101-2020_11_24_390039 57 29 considered consider VBN 10_1101-2020_11_24_390039 57 30 “ " `` 10_1101-2020_11_24_390039 57 31 type type NN 10_1101-2020_11_24_390039 57 32 III III NNP 10_1101-2020_11_24_390039 57 33 - - HYPH 10_1101-2020_11_24_390039 57 34 like like JJ 10_1101-2020_11_24_390039 57 35 ” " '' 10_1101-2020_11_24_390039 57 36 . . . 10_1101-2020_11_24_390039 58 1 Although although IN 10_1101-2020_11_24_390039 58 2 human human JJ 10_1101-2020_11_24_390039 58 3 multi multi JJ 10_1101-2020_11_24_390039 58 4 - - JJ 10_1101-2020_11_24_390039 58 5 pass pass JJ 10_1101-2020_11_24_390039 58 6 TMPs tmp NNS 10_1101-2020_11_24_390039 58 7 of of IN 10_1101-2020_11_24_390039 58 8 this this DT 10_1101-2020_11_24_390039 58 9 type type NN 10_1101-2020_11_24_390039 58 10 typically typically RB 10_1101-2020_11_24_390039 58 11 require require VBP 10_1101-2020_11_24_390039 58 12 both both CC 10_1101-2020_11_24_390039 58 13 the the DT 10_1101-2020_11_24_390039 58 14 ER ER NNP 10_1101-2020_11_24_390039 58 15 membrane membrane NN 10_1101-2020_11_24_390039 58 16 complex complex NN 10_1101-2020_11_24_390039 58 17 ( ( -LRB- 10_1101-2020_11_24_390039 58 18 EMC EMC NNP 10_1101-2020_11_24_390039 58 19 ) ) -RRB- 10_1101-2020_11_24_390039 58 20 and and CC 10_1101-2020_11_24_390039 58 21 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 58 22 translocon translocon NN 10_1101-2020_11_24_390039 58 23 for for IN 10_1101-2020_11_24_390039 58 24 their -PRON- PRP$ 10_1101-2020_11_24_390039 58 25 authentic authentic JJ 10_1101-2020_11_24_390039 58 26 ER ER NNP 10_1101-2020_11_24_390039 58 27 insertion insertion NN 10_1101-2020_11_24_390039 58 28 ( ( -LRB- 10_1101-2020_11_24_390039 58 29 Chitwood Chitwood NNP 10_1101-2020_11_24_390039 58 30 et et NNP 10_1101-2020_11_24_390039 58 31 al al NNP 10_1101-2020_11_24_390039 58 32 . . NNP 10_1101-2020_11_24_390039 58 33 , , , 10_1101-2020_11_24_390039 58 34 2018 2018 CD 10_1101-2020_11_24_390039 58 35 ) ) -RRB- 10_1101-2020_11_24_390039 58 36 , , , 10_1101-2020_11_24_390039 58 37 Ipom Ipom NNP 10_1101-2020_11_24_390039 58 38 - - HYPH 10_1101-2020_11_24_390039 58 39 F F NNP 10_1101-2020_11_24_390039 58 40 had have VBD 10_1101-2020_11_24_390039 58 41 no no DT 10_1101-2020_11_24_390039 58 42 significant significant JJ 10_1101-2020_11_24_390039 58 43 effect effect NN 10_1101-2020_11_24_390039 58 44 on on IN 10_1101-2020_11_24_390039 58 45 the the DT 10_1101-2020_11_24_390039 58 46 ER ER NNP 10_1101-2020_11_24_390039 58 47 translocation translocation NN 10_1101-2020_11_24_390039 58 48 / / SYM 10_1101-2020_11_24_390039 58 49 insertion insertion NN 10_1101-2020_11_24_390039 58 50 of of IN 10_1101-2020_11_24_390039 58 51 the the DT 10_1101-2020_11_24_390039 58 52 M M NNP 10_1101-2020_11_24_390039 58 53 protein protein NN 10_1101-2020_11_24_390039 58 54 in in IN 10_1101-2020_11_24_390039 58 55 vitro vitro FW 10_1101-2020_11_24_390039 58 56 , , , 10_1101-2020_11_24_390039 58 57 as as IN 10_1101-2020_11_24_390039 58 58 judged judge VBN 10_1101-2020_11_24_390039 58 59 by by IN 10_1101-2020_11_24_390039 58 60 the the DT 10_1101-2020_11_24_390039 58 61 efficiency efficiency NN 10_1101-2020_11_24_390039 58 62 of of IN 10_1101-2020_11_24_390039 58 63 N- n- CD 10_1101-2020_11_24_390039 58 64 glycosylation glycosylation NN 10_1101-2020_11_24_390039 58 65 of of IN 10_1101-2020_11_24_390039 58 66 its -PRON- PRP$ 10_1101-2020_11_24_390039 58 67 N n CD 10_1101-2020_11_24_390039 58 68 - - HYPH 10_1101-2020_11_24_390039 58 69 terminal terminal NN 10_1101-2020_11_24_390039 58 70 domain domain NN 10_1101-2020_11_24_390039 58 71 ( ( -LRB- 10_1101-2020_11_24_390039 58 72 Fig fig NN 10_1101-2020_11_24_390039 58 73 . . . 10_1101-2020_11_24_390039 59 1 2C 2C NNP 10_1101-2020_11_24_390039 59 2 ) ) -RRB- 10_1101-2020_11_24_390039 59 3 . . . 10_1101-2020_11_24_390039 60 1 We -PRON- PRP 10_1101-2020_11_24_390039 60 2 conclude conclude VBP 10_1101-2020_11_24_390039 60 3 that that IN 10_1101-2020_11_24_390039 60 4 the the DT 10_1101-2020_11_24_390039 60 5 integration integration NN 10_1101-2020_11_24_390039 60 6 of of IN 10_1101-2020_11_24_390039 60 7 its -PRON- PRP$ 10_1101-2020_11_24_390039 60 8 first first JJ 10_1101-2020_11_24_390039 60 9 TMD TMD NNP 10_1101-2020_11_24_390039 60 10 is be VBZ 10_1101-2020_11_24_390039 60 11 unaffected unaffected JJ 10_1101-2020_11_24_390039 60 12 by by IN 10_1101-2020_11_24_390039 60 13 Ipom Ipom NNP 10_1101-2020_11_24_390039 60 14 - - HYPH 10_1101-2020_11_24_390039 60 15 F F NNP 10_1101-2020_11_24_390039 60 16 , , , 10_1101-2020_11_24_390039 60 17 consistent consistent JJ 10_1101-2020_11_24_390039 60 18 with with IN 10_1101-2020_11_24_390039 60 19 its -PRON- PRP$ 10_1101-2020_11_24_390039 60 20 use use NN 10_1101-2020_11_24_390039 60 21 of of IN 10_1101-2020_11_24_390039 60 22 the the DT 10_1101-2020_11_24_390039 60 23 EMC EMC NNP 10_1101-2020_11_24_390039 60 24 ( ( -LRB- 10_1101-2020_11_24_390039 60 25 Chitwood Chitwood NNP 10_1101-2020_11_24_390039 60 26 et et NNP 10_1101-2020_11_24_390039 60 27 al al NNP 10_1101-2020_11_24_390039 60 28 . . NNP 10_1101-2020_11_24_390039 60 29 , , , 10_1101-2020_11_24_390039 60 30 2018 2018 CD 10_1101-2020_11_24_390039 60 31 ; ; : 10_1101-2020_11_24_390039 60 32 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 60 33 et et NNP 10_1101-2020_11_24_390039 60 34 al al NNP 10_1101-2020_11_24_390039 60 35 . . NNP 10_1101-2020_11_24_390039 60 36 , , , 10_1101-2020_11_24_390039 60 37 2020 2020 CD 10_1101-2020_11_24_390039 60 38 submitted submit VBN 10_1101-2020_11_24_390039 60 39 ) ) -RRB- 10_1101-2020_11_24_390039 60 40 . . . 10_1101-2020_11_24_390039 61 1 There there EX 10_1101-2020_11_24_390039 61 2 is be VBZ 10_1101-2020_11_24_390039 61 3 however however RB 10_1101-2020_11_24_390039 61 4 a a DT 10_1101-2020_11_24_390039 61 5 qualitative qualitative JJ 10_1101-2020_11_24_390039 61 6 reduction reduction NN 10_1101-2020_11_24_390039 61 7 in in IN 10_1101-2020_11_24_390039 61 8 the the DT 10_1101-2020_11_24_390039 61 9 intensity intensity NN 10_1101-2020_11_24_390039 61 10 of of IN 10_1101-2020_11_24_390039 61 11 both both CC 10_1101-2020_11_24_390039 61 12 the the DT 10_1101-2020_11_24_390039 61 13 non- non- NN 10_1101-2020_11_24_390039 61 14 and and CC 10_1101-2020_11_24_390039 61 15 N N NNP 10_1101-2020_11_24_390039 61 16 - - HYPH 10_1101-2020_11_24_390039 61 17 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 61 18 forms form NNS 10_1101-2020_11_24_390039 61 19 of of IN 10_1101-2020_11_24_390039 61 20 the the DT 10_1101-2020_11_24_390039 61 21 M M NNP 10_1101-2020_11_24_390039 61 22 protein protein NN 10_1101-2020_11_24_390039 61 23 when when WRB 10_1101-2020_11_24_390039 61 24 compared compare VBN 10_1101-2020_11_24_390039 61 25 to to IN 10_1101-2020_11_24_390039 61 26 the the DT 10_1101-2020_11_24_390039 61 27 control control NN 10_1101-2020_11_24_390039 61 28 ( ( -LRB- 10_1101-2020_11_24_390039 61 29 see see VB 10_1101-2020_11_24_390039 61 30 Fig Fig NNP 10_1101-2020_11_24_390039 61 31 . . . 10_1101-2020_11_24_390039 62 1 2B 2B NNP 10_1101-2020_11_24_390039 62 2 and and CC 10_1101-2020_11_24_390039 62 3 Fig Fig NNP 10_1101-2020_11_24_390039 62 4 . . . 10_1101-2020_11_24_390039 63 1 S1A s1a LS 10_1101-2020_11_24_390039 63 2 ) ) -RRB- 10_1101-2020_11_24_390039 63 3 . . . 10_1101-2020_11_24_390039 64 1 We -PRON- PRP 10_1101-2020_11_24_390039 64 2 speculate speculate VBP 10_1101-2020_11_24_390039 64 3 that that IN 10_1101-2020_11_24_390039 64 4 this this DT 10_1101-2020_11_24_390039 64 5 decrease decrease NN 10_1101-2020_11_24_390039 64 6 may may MD 10_1101-2020_11_24_390039 64 7 reflect reflect VB 10_1101-2020_11_24_390039 64 8 an an DT 10_1101-2020_11_24_390039 64 9 Ipom Ipom NNP 10_1101-2020_11_24_390039 64 10 - - HYPH 10_1101-2020_11_24_390039 64 11 F F NNP 10_1101-2020_11_24_390039 64 12 - - HYPH 10_1101-2020_11_24_390039 64 13 induced induce VBN 10_1101-2020_11_24_390039 64 14 effect effect NN 10_1101-2020_11_24_390039 64 15 on on IN 10_1101-2020_11_24_390039 64 16 the the DT 10_1101-2020_11_24_390039 64 17 Sec61- sec61- XX 10_1101-2020_11_24_390039 64 18 dependent dependent JJ 10_1101-2020_11_24_390039 64 19 integration integration NN 10_1101-2020_11_24_390039 64 20 of of IN 10_1101-2020_11_24_390039 64 21 the the DT 10_1101-2020_11_24_390039 64 22 second second JJ 10_1101-2020_11_24_390039 64 23 and/or and/or CC 10_1101-2020_11_24_390039 64 24 third third JJ 10_1101-2020_11_24_390039 64 25 TM TM NNP 10_1101-2020_11_24_390039 64 26 - - HYPH 10_1101-2020_11_24_390039 64 27 spans span NNS 10_1101-2020_11_24_390039 64 28 of of IN 10_1101-2020_11_24_390039 64 29 the the DT 10_1101-2020_11_24_390039 64 30 M M NNP 10_1101-2020_11_24_390039 64 31 protein protein NN 10_1101-2020_11_24_390039 64 32 ( ( -LRB- 10_1101-2020_11_24_390039 64 33 cf cf NN 10_1101-2020_11_24_390039 64 34 . . . 10_1101-2020_11_24_390039 65 1 Chitwood Chitwood NNP 10_1101-2020_11_24_390039 65 2 et et NNP 10_1101-2020_11_24_390039 65 3 al al NNP 10_1101-2020_11_24_390039 65 4 . . NNP 10_1101-2020_11_24_390039 65 5 , , , 10_1101-2020_11_24_390039 65 6 2018 2018 CD 10_1101-2020_11_24_390039 65 7 ) ) -RRB- 10_1101-2020_11_24_390039 65 8 and and CC 10_1101-2020_11_24_390039 65 9 our -PRON- PRP$ 10_1101-2020_11_24_390039 65 10 future future JJ 10_1101-2020_11_24_390039 65 11 studies study NNS 10_1101-2020_11_24_390039 65 12 will will MD 10_1101-2020_11_24_390039 65 13 aim aim VB 10_1101-2020_11_24_390039 65 14 to to TO 10_1101-2020_11_24_390039 65 15 resolve resolve VB 10_1101-2020_11_24_390039 65 16 this this DT 10_1101-2020_11_24_390039 65 17 question question NN 10_1101-2020_11_24_390039 65 18 . . . 10_1101-2020_11_24_390039 66 1 Nevertheless nevertheless RB 10_1101-2020_11_24_390039 66 2 , , , 10_1101-2020_11_24_390039 66 3 like like IN 10_1101-2020_11_24_390039 66 4 similar similar JJ 10_1101-2020_11_24_390039 66 5 host host NN 10_1101-2020_11_24_390039 66 6 cell cell NN 10_1101-2020_11_24_390039 66 7 multi multi JJ 10_1101-2020_11_24_390039 66 8 - - JJ 10_1101-2020_11_24_390039 66 9 pass pass JJ 10_1101-2020_11_24_390039 66 10 TMPs tmp NNS 10_1101-2020_11_24_390039 66 11 that that WDT 10_1101-2020_11_24_390039 66 12 are be VBP 10_1101-2020_11_24_390039 66 13 resistant resistant JJ 10_1101-2020_11_24_390039 66 14 to to IN 10_1101-2020_11_24_390039 66 15 a a DT 10_1101-2020_11_24_390039 66 16 similar similar JJ 10_1101-2020_11_24_390039 66 17 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 66 18 inhibitor inhibitor NN 10_1101-2020_11_24_390039 66 19 mycolactone mycolactone NN 10_1101-2020_11_24_390039 66 20 ( ( -LRB- 10_1101-2020_11_24_390039 66 21 Morel Morel NNP 10_1101-2020_11_24_390039 66 22 et et NNP 10_1101-2020_11_24_390039 66 23 al al NNP 10_1101-2020_11_24_390039 66 24 . . NNP 10_1101-2020_11_24_390039 66 25 , , , 10_1101-2020_11_24_390039 66 26 2018 2018 CD 10_1101-2020_11_24_390039 66 27 ) ) -RRB- 10_1101-2020_11_24_390039 66 28 , , , 10_1101-2020_11_24_390039 66 29 the the DT 10_1101-2020_11_24_390039 66 30 M M NNP 10_1101-2020_11_24_390039 66 31 protein protein NN 10_1101-2020_11_24_390039 66 32 appears appear VBZ 10_1101-2020_11_24_390039 66 33 more more RBR 10_1101-2020_11_24_390039 66 34 resistant resistant JJ 10_1101-2020_11_24_390039 66 35 to to IN 10_1101-2020_11_24_390039 66 36 Ipom Ipom NNP 10_1101-2020_11_24_390039 66 37 - - HYPH 10_1101-2020_11_24_390039 66 38 F F NNP 10_1101-2020_11_24_390039 66 39 than than IN 10_1101-2020_11_24_390039 66 40 either either CC 10_1101-2020_11_24_390039 66 41 the the DT 10_1101-2020_11_24_390039 66 42 S S NNP 10_1101-2020_11_24_390039 66 43 or or CC 10_1101-2020_11_24_390039 66 44 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 66 45 proteins protein NNS 10_1101-2020_11_24_390039 66 46 ( ( -LRB- 10_1101-2020_11_24_390039 66 47 Fig fig NN 10_1101-2020_11_24_390039 66 48 2 2 CD 10_1101-2020_11_24_390039 66 49 , , , 10_1101-2020_11_24_390039 66 50 Fig Fig NNP 10_1101-2020_11_24_390039 66 51 . . . 10_1101-2020_11_24_390039 67 1 S1A s1a LS 10_1101-2020_11_24_390039 67 2 ) ) -RRB- 10_1101-2020_11_24_390039 67 3 . . . 10_1101-2020_11_24_390039 68 1 In in IN 10_1101-2020_11_24_390039 68 2 practice practice NN 10_1101-2020_11_24_390039 68 3 , , , 10_1101-2020_11_24_390039 68 4 the the DT 10_1101-2020_11_24_390039 68 5 potential potential JJ 10_1101-2020_11_24_390039 68 6 resistance resistance NN 10_1101-2020_11_24_390039 68 7 of of IN 10_1101-2020_11_24_390039 68 8 this this DT 10_1101-2020_11_24_390039 68 9 highly highly RB 10_1101-2020_11_24_390039 68 10 abundant abundant JJ 10_1101-2020_11_24_390039 68 11 and and CC 10_1101-2020_11_24_390039 68 12 functionally functionally RB 10_1101-2020_11_24_390039 68 13 diverse diverse JJ 10_1101-2020_11_24_390039 68 14 class class NN 10_1101-2020_11_24_390039 68 15 of of IN 10_1101-2020_11_24_390039 68 16 endogenous endogenous JJ 10_1101-2020_11_24_390039 68 17 multi multi JJ 10_1101-2020_11_24_390039 68 18 - - JJ 10_1101-2020_11_24_390039 68 19 spanning span VBG 10_1101-2020_11_24_390039 68 20 membrane membrane NN 10_1101-2020_11_24_390039 68 21 proteins protein NNS 10_1101-2020_11_24_390039 68 22 ( ( -LRB- 10_1101-2020_11_24_390039 68 23 von von NNP 10_1101-2020_11_24_390039 68 24 Heijne Heijne NNP 10_1101-2020_11_24_390039 68 25 , , , 10_1101-2020_11_24_390039 68 26 2007 2007 CD 10_1101-2020_11_24_390039 68 27 ) ) -RRB- 10_1101-2020_11_24_390039 68 28 may may MD 10_1101-2020_11_24_390039 68 29 limit limit VB 10_1101-2020_11_24_390039 68 30 any any DT 10_1101-2020_11_24_390039 68 31 Ipom Ipom NNP 10_1101-2020_11_24_390039 68 32 - - HYPH 10_1101-2020_11_24_390039 68 33 F F NNP 10_1101-2020_11_24_390039 68 34 - - HYPH 10_1101-2020_11_24_390039 68 35 induced induce VBN 10_1101-2020_11_24_390039 68 36 cytotoxicity cytotoxicity NN 10_1101-2020_11_24_390039 68 37 towards towards IN 10_1101-2020_11_24_390039 68 38 host host NN 10_1101-2020_11_24_390039 68 39 cells cell NNS 10_1101-2020_11_24_390039 68 40 . . . 10_1101-2020_11_24_390039 69 1 .CC .CC NFP 10_1101-2020_11_24_390039 69 2 - - : 10_1101-2020_11_24_390039 69 3 BY by IN 10_1101-2020_11_24_390039 69 4 - - HYPH 10_1101-2020_11_24_390039 69 5 ND ND NNP 10_1101-2020_11_24_390039 69 6 4.0 4.0 CD 10_1101-2020_11_24_390039 69 7 International international JJ 10_1101-2020_11_24_390039 69 8 licensemade licensemade NN 10_1101-2020_11_24_390039 69 9 available available JJ 10_1101-2020_11_24_390039 69 10 under under IN 10_1101-2020_11_24_390039 69 11 a a DT 10_1101-2020_11_24_390039 69 12 ( ( -LRB- 10_1101-2020_11_24_390039 69 13 which which WDT 10_1101-2020_11_24_390039 69 14 was be VBD 10_1101-2020_11_24_390039 69 15 not not RB 10_1101-2020_11_24_390039 69 16 certified certify VBN 10_1101-2020_11_24_390039 69 17 by by IN 10_1101-2020_11_24_390039 69 18 peer peer NN 10_1101-2020_11_24_390039 69 19 review review NN 10_1101-2020_11_24_390039 69 20 ) ) -RRB- 10_1101-2020_11_24_390039 69 21 is be VBZ 10_1101-2020_11_24_390039 69 22 the the DT 10_1101-2020_11_24_390039 69 23 author author NN 10_1101-2020_11_24_390039 69 24 / / SYM 10_1101-2020_11_24_390039 69 25 funder funder NN 10_1101-2020_11_24_390039 69 26 , , , 10_1101-2020_11_24_390039 69 27 who who WP 10_1101-2020_11_24_390039 69 28 has have VBZ 10_1101-2020_11_24_390039 69 29 granted grant VBN 10_1101-2020_11_24_390039 69 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 69 31 a a DT 10_1101-2020_11_24_390039 69 32 license license NN 10_1101-2020_11_24_390039 69 33 to to TO 10_1101-2020_11_24_390039 69 34 display display VB 10_1101-2020_11_24_390039 69 35 the the DT 10_1101-2020_11_24_390039 69 36 preprint preprint NN 10_1101-2020_11_24_390039 69 37 in in IN 10_1101-2020_11_24_390039 69 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 69 39 . . . 10_1101-2020_11_24_390039 70 1 It -PRON- PRP 10_1101-2020_11_24_390039 70 2 is be VBZ 10_1101-2020_11_24_390039 70 3 The the DT 10_1101-2020_11_24_390039 70 4 copyright copyright NN 10_1101-2020_11_24_390039 70 5 holder holder NN 10_1101-2020_11_24_390039 70 6 for for IN 10_1101-2020_11_24_390039 70 7 this this DT 10_1101-2020_11_24_390039 70 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 70 9 version version NN 10_1101-2020_11_24_390039 70 10 posted post VBD 10_1101-2020_11_24_390039 70 11 January January NNP 10_1101-2020_11_24_390039 70 12 5 5 CD 10_1101-2020_11_24_390039 70 13 , , , 10_1101-2020_11_24_390039 70 14 2021 2021 CD 10_1101-2020_11_24_390039 70 15 . . . 10_1101-2020_11_24_390039 70 16 ; ; : 10_1101-2020_11_24_390039 70 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 70 18 : : : 10_1101-2020_11_24_390039 70 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 70 20 preprint preprint JJ 10_1101-2020_11_24_390039 70 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 70 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 70 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 70 24 - - HYPH 10_1101-2020_11_24_390039 70 25 F F NNP 10_1101-2020_11_24_390039 70 26 as as IN 10_1101-2020_11_24_390039 70 27 a a DT 10_1101-2020_11_24_390039 70 28 potential potential JJ 10_1101-2020_11_24_390039 70 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 70 30 agent agent NN 10_1101-2020_11_24_390039 70 31 Page Page NNP 10_1101-2020_11_24_390039 70 32 6 6 CD 10_1101-2020_11_24_390039 70 33 of of IN 10_1101-2020_11_24_390039 70 34 21 21 CD 10_1101-2020_11_24_390039 70 35 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 70 36 assumes assume VBZ 10_1101-2020_11_24_390039 70 37 a a DT 10_1101-2020_11_24_390039 70 38 lumenal lumenal NN 10_1101-2020_11_24_390039 70 39 - - HYPH 10_1101-2020_11_24_390039 70 40 facing face VBG 10_1101-2020_11_24_390039 70 41 hairpin hairpin NN 10_1101-2020_11_24_390039 70 42 topology topology NN 10_1101-2020_11_24_390039 70 43 in in IN 10_1101-2020_11_24_390039 70 44 ER ER NNP 10_1101-2020_11_24_390039 70 45 - - HYPH 10_1101-2020_11_24_390039 70 46 derived derive VBN 10_1101-2020_11_24_390039 70 47 microsomes microsome NNS 10_1101-2020_11_24_390039 70 48 Cell cell NN 10_1101-2020_11_24_390039 70 49 - - HYPH 10_1101-2020_11_24_390039 70 50 based base VBN 10_1101-2020_11_24_390039 70 51 studies study NNS 10_1101-2020_11_24_390039 70 52 of of IN 10_1101-2020_11_24_390039 70 53 the the DT 10_1101-2020_11_24_390039 70 54 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 70 55 protein protein NN 10_1101-2020_11_24_390039 70 56 from from IN 10_1101-2020_11_24_390039 70 57 SARS SARS NNP 10_1101-2020_11_24_390039 70 58 - - HYPH 10_1101-2020_11_24_390039 70 59 CoV-1 CoV-1 NNP 10_1101-2020_11_24_390039 70 60 suggest suggest VBP 10_1101-2020_11_24_390039 70 61 it -PRON- PRP 10_1101-2020_11_24_390039 70 62 has have VBZ 10_1101-2020_11_24_390039 70 63 an an DT 10_1101-2020_11_24_390039 70 64 unusual unusual JJ 10_1101-2020_11_24_390039 70 65 hairpin hairpin NN 10_1101-2020_11_24_390039 70 66 topology topology NN 10_1101-2020_11_24_390039 70 67 with with IN 10_1101-2020_11_24_390039 70 68 both both CC 10_1101-2020_11_24_390039 70 69 its -PRON- PRP$ 10_1101-2020_11_24_390039 70 70 N- N- NNP 10_1101-2020_11_24_390039 70 71 and and CC 10_1101-2020_11_24_390039 70 72 C C NNP 10_1101-2020_11_24_390039 70 73 - - HYPH 10_1101-2020_11_24_390039 70 74 termini termini NNP 10_1101-2020_11_24_390039 70 75 located locate VBN 10_1101-2020_11_24_390039 70 76 on on IN 10_1101-2020_11_24_390039 70 77 the the DT 10_1101-2020_11_24_390039 70 78 exoplasmic exoplasmic JJ 10_1101-2020_11_24_390039 70 79 side side NN 10_1101-2020_11_24_390039 70 80 of of IN 10_1101-2020_11_24_390039 70 81 the the DT 10_1101-2020_11_24_390039 70 82 host host NN 10_1101-2020_11_24_390039 70 83 cell cell NN 10_1101-2020_11_24_390039 70 84 membrane membrane NN 10_1101-2020_11_24_390039 70 85 , , , 10_1101-2020_11_24_390039 70 86 to to TO 10_1101-2020_11_24_390039 70 87 which which WDT 10_1101-2020_11_24_390039 70 88 it -PRON- PRP 10_1101-2020_11_24_390039 70 89 binds bind VBZ 10_1101-2020_11_24_390039 70 90 via via IN 10_1101-2020_11_24_390039 70 91 an an DT 10_1101-2020_11_24_390039 70 92 N N NNP 10_1101-2020_11_24_390039 70 93 - - HYPH 10_1101-2020_11_24_390039 70 94 terminal terminal NN 10_1101-2020_11_24_390039 70 95 amphipathic amphipathic JJ 10_1101-2020_11_24_390039 70 96 helix helix NN 10_1101-2020_11_24_390039 70 97 ( ( -LRB- 10_1101-2020_11_24_390039 70 98 Netland Netland NNP 10_1101-2020_11_24_390039 70 99 et et NNP 10_1101-2020_11_24_390039 70 100 al al NNP 10_1101-2020_11_24_390039 70 101 . . NNP 10_1101-2020_11_24_390039 70 102 , , , 10_1101-2020_11_24_390039 70 103 2007 2007 CD 10_1101-2020_11_24_390039 70 104 ) ) -RRB- 10_1101-2020_11_24_390039 70 105 . . . 10_1101-2020_11_24_390039 71 1 To to TO 10_1101-2020_11_24_390039 71 2 independently independently RB 10_1101-2020_11_24_390039 71 3 determine determine VB 10_1101-2020_11_24_390039 71 4 the the DT 10_1101-2020_11_24_390039 71 5 membrane membrane NN 10_1101-2020_11_24_390039 71 6 topology topology NN 10_1101-2020_11_24_390039 71 7 of of IN 10_1101-2020_11_24_390039 71 8 SARS SARS NNP 10_1101-2020_11_24_390039 71 9 - - HYPH 10_1101-2020_11_24_390039 71 10 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 71 11 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 71 12 , , , 10_1101-2020_11_24_390039 71 13 we -PRON- PRP 10_1101-2020_11_24_390039 71 14 prepared prepare VBD 10_1101-2020_11_24_390039 71 15 versions version NNS 10_1101-2020_11_24_390039 71 16 with with IN 10_1101-2020_11_24_390039 71 17 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 71 18 tags tag NNS 10_1101-2020_11_24_390039 71 19 at at IN 10_1101-2020_11_24_390039 71 20 both both CC 10_1101-2020_11_24_390039 71 21 its -PRON- PRP$ 10_1101-2020_11_24_390039 71 22 N- N- NNP 10_1101-2020_11_24_390039 71 23 and and CC 10_1101-2020_11_24_390039 71 24 C C NNP 10_1101-2020_11_24_390039 71 25 - - HYPH 10_1101-2020_11_24_390039 71 26 termini termini NNP 10_1101-2020_11_24_390039 71 27 , , , 10_1101-2020_11_24_390039 71 28 or or CC 10_1101-2020_11_24_390039 71 29 single single JJ 10_1101-2020_11_24_390039 71 30 tagged tag VBN 10_1101-2020_11_24_390039 71 31 equivalents equivalent NNS 10_1101-2020_11_24_390039 71 32 ( ( -LRB- 10_1101-2020_11_24_390039 71 33 see see VB 10_1101-2020_11_24_390039 71 34 Fig Fig NNP 10_1101-2020_11_24_390039 71 35 . . . 10_1101-2020_11_24_390039 72 1 2B 2B NNP 10_1101-2020_11_24_390039 72 2 , , , 10_1101-2020_11_24_390039 72 3 schematics schematic NNS 10_1101-2020_11_24_390039 72 4 , , , 10_1101-2020_11_24_390039 72 5 OPG2-ORF6- OPG2-ORF6- NNP 10_1101-2020_11_24_390039 72 6 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 72 7 , , , 10_1101-2020_11_24_390039 72 8 OPG2-ORF6 OPG2-ORF6 NNP 10_1101-2020_11_24_390039 72 9 and and CC 10_1101-2020_11_24_390039 72 10 ORF6-OPG2 ORF6-OPG2 NNP 10_1101-2020_11_24_390039 72 11 ) ) -RRB- 10_1101-2020_11_24_390039 72 12 . . . 10_1101-2020_11_24_390039 73 1 Following follow VBG 10_1101-2020_11_24_390039 73 2 membrane membrane NN 10_1101-2020_11_24_390039 73 3 insertion insertion NN 10_1101-2020_11_24_390039 73 4 , , , 10_1101-2020_11_24_390039 73 5 doubly doubly RB 10_1101-2020_11_24_390039 73 6 tagged tag VBD 10_1101-2020_11_24_390039 73 7 OPG2-ORF6-OPG2 OPG2-ORF6-OPG2 NNP 10_1101-2020_11_24_390039 73 8 shows show VBZ 10_1101-2020_11_24_390039 73 9 significant significant JJ 10_1101-2020_11_24_390039 73 10 amounts amount NNS 10_1101-2020_11_24_390039 73 11 of of IN 10_1101-2020_11_24_390039 73 12 species specie NNS 10_1101-2020_11_24_390039 73 13 with with IN 10_1101-2020_11_24_390039 73 14 3- 3- NNPS 10_1101-2020_11_24_390039 73 15 and and CC 10_1101-2020_11_24_390039 73 16 4- 4- NNP 10_1101-2020_11_24_390039 73 17 N N NNP 10_1101-2020_11_24_390039 73 18 - - HYPH 10_1101-2020_11_24_390039 73 19 linked link VBN 10_1101-2020_11_24_390039 73 20 glycans glycan NNS 10_1101-2020_11_24_390039 73 21 ( ( -LRB- 10_1101-2020_11_24_390039 73 22 Fig fig NN 10_1101-2020_11_24_390039 73 23 . . . 10_1101-2020_11_24_390039 74 1 2B 2B NNP 10_1101-2020_11_24_390039 74 2 ) ) -RRB- 10_1101-2020_11_24_390039 74 3 . . . 10_1101-2020_11_24_390039 75 1 This this DT 10_1101-2020_11_24_390039 75 2 pattern pattern NN 10_1101-2020_11_24_390039 75 3 confirms confirm VBZ 10_1101-2020_11_24_390039 75 4 that that IN 10_1101-2020_11_24_390039 75 5 the the DT 10_1101-2020_11_24_390039 75 6 SARS SARS NNP 10_1101-2020_11_24_390039 75 7 - - HYPH 10_1101-2020_11_24_390039 75 8 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 75 9 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 75 10 protein protein NN 10_1101-2020_11_24_390039 75 11 assumes assume VBZ 10_1101-2020_11_24_390039 75 12 a a DT 10_1101-2020_11_24_390039 75 13 ‘ ' `` 10_1101-2020_11_24_390039 75 14 hairpin hairpin NN 10_1101-2020_11_24_390039 75 15 ’ ' '' 10_1101-2020_11_24_390039 75 16 conformation conformation NN 10_1101-2020_11_24_390039 75 17 in in IN 10_1101-2020_11_24_390039 75 18 the the DT 10_1101-2020_11_24_390039 75 19 ER ER NNP 10_1101-2020_11_24_390039 75 20 membrane membrane NN 10_1101-2020_11_24_390039 75 21 with with IN 10_1101-2020_11_24_390039 75 22 both both CC 10_1101-2020_11_24_390039 75 23 its -PRON- PRP$ 10_1101-2020_11_24_390039 75 24 N- N- NNP 10_1101-2020_11_24_390039 75 25 and and CC 10_1101-2020_11_24_390039 75 26 C C NNP 10_1101-2020_11_24_390039 75 27 - - HYPH 10_1101-2020_11_24_390039 75 28 termini termini NNP 10_1101-2020_11_24_390039 75 29 in in IN 10_1101-2020_11_24_390039 75 30 the the DT 10_1101-2020_11_24_390039 75 31 lumen luman NNS 10_1101-2020_11_24_390039 75 32 ( ( -LRB- 10_1101-2020_11_24_390039 75 33 Fig fig NN 10_1101-2020_11_24_390039 75 34 . . . 10_1101-2020_11_24_390039 76 1 2B 2B NNP 10_1101-2020_11_24_390039 76 2 , , , 10_1101-2020_11_24_390039 76 3 OPG2-ORF6-OPG2 OPG2-ORF6-OPG2 NNP 10_1101-2020_11_24_390039 76 4 ) ) -RRB- 10_1101-2020_11_24_390039 76 5 . . . 10_1101-2020_11_24_390039 77 1 These these DT 10_1101-2020_11_24_390039 77 2 3- 3- NNS 10_1101-2020_11_24_390039 77 3 and and CC 10_1101-2020_11_24_390039 77 4 4-N- 4-n- CD 10_1101-2020_11_24_390039 77 5 glycan glycan JJ 10_1101-2020_11_24_390039 77 6 bearing bear VBG 10_1101-2020_11_24_390039 77 7 OPG2-ORF6-OPG2 OPG2-ORF6-OPG2 NNP 10_1101-2020_11_24_390039 77 8 species specie NNS 10_1101-2020_11_24_390039 77 9 are be VBP 10_1101-2020_11_24_390039 77 10 also also RB 10_1101-2020_11_24_390039 77 11 resistant resistant JJ 10_1101-2020_11_24_390039 77 12 to to TO 10_1101-2020_11_24_390039 77 13 extraction extraction VB 10_1101-2020_11_24_390039 77 14 with with IN 10_1101-2020_11_24_390039 77 15 alkaline alkaline NN 10_1101-2020_11_24_390039 77 16 sodium sodium NN 10_1101-2020_11_24_390039 77 17 carbonate carbonate NN 10_1101-2020_11_24_390039 77 18 buffer buffer NN 10_1101-2020_11_24_390039 77 19 ( ( -LRB- 10_1101-2020_11_24_390039 77 20 Fig fig NN 10_1101-2020_11_24_390039 77 21 . . . 10_1101-2020_11_24_390039 78 1 S1D s1d LS 10_1101-2020_11_24_390039 78 2 ) ) -RRB- 10_1101-2020_11_24_390039 78 3 and and CC 10_1101-2020_11_24_390039 78 4 protected protect VBN 10_1101-2020_11_24_390039 78 5 from from IN 10_1101-2020_11_24_390039 78 6 added add VBN 10_1101-2020_11_24_390039 78 7 protease protease NN 10_1101-2020_11_24_390039 78 8 ( ( -LRB- 10_1101-2020_11_24_390039 78 9 Fig fig NN 10_1101-2020_11_24_390039 78 10 . . . 10_1101-2020_11_24_390039 79 1 S1E s1e LS 10_1101-2020_11_24_390039 79 2 ) ) -RRB- 10_1101-2020_11_24_390039 79 3 , , , 10_1101-2020_11_24_390039 79 4 further further RB 10_1101-2020_11_24_390039 79 5 indicating indicate VBG 10_1101-2020_11_24_390039 79 6 that that IN 10_1101-2020_11_24_390039 79 7 the the DT 10_1101-2020_11_24_390039 79 8 majority majority NN 10_1101-2020_11_24_390039 79 9 of of IN 10_1101-2020_11_24_390039 79 10 the the DT 10_1101-2020_11_24_390039 79 11 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 79 12 protein protein NN 10_1101-2020_11_24_390039 79 13 is be VBZ 10_1101-2020_11_24_390039 79 14 stably stably RB 10_1101-2020_11_24_390039 79 15 associated associate VBN 10_1101-2020_11_24_390039 79 16 with with IN 10_1101-2020_11_24_390039 79 17 the the DT 10_1101-2020_11_24_390039 79 18 ER ER NNP 10_1101-2020_11_24_390039 79 19 membrane membrane NN 10_1101-2020_11_24_390039 79 20 in in IN 10_1101-2020_11_24_390039 79 21 a a DT 10_1101-2020_11_24_390039 79 22 ‘ ' `` 10_1101-2020_11_24_390039 79 23 hairpin hairpin NN 10_1101-2020_11_24_390039 79 24 ’ ' '' 10_1101-2020_11_24_390039 79 25 ( ( -LRB- 10_1101-2020_11_24_390039 79 26 Nexo Nexo NNP 10_1101-2020_11_24_390039 79 27 / / SYM 10_1101-2020_11_24_390039 79 28 Cexo Cexo NNP 10_1101-2020_11_24_390039 79 29 ) ) -RRB- 10_1101-2020_11_24_390039 79 30 topology topology NN 10_1101-2020_11_24_390039 79 31 . . . 10_1101-2020_11_24_390039 80 1 Consistent consistent JJ 10_1101-2020_11_24_390039 80 2 with with IN 10_1101-2020_11_24_390039 80 3 this this DT 10_1101-2020_11_24_390039 80 4 unusual unusual JJ 10_1101-2020_11_24_390039 80 5 membrane membrane NN 10_1101-2020_11_24_390039 80 6 topology topology NN 10_1101-2020_11_24_390039 80 7 , , , 10_1101-2020_11_24_390039 80 8 we -PRON- PRP 10_1101-2020_11_24_390039 80 9 find find VBP 10_1101-2020_11_24_390039 80 10 no no DT 10_1101-2020_11_24_390039 80 11 indication indication NN 10_1101-2020_11_24_390039 80 12 that that IN 10_1101-2020_11_24_390039 80 13 the the DT 10_1101-2020_11_24_390039 80 14 membrane membrane NN 10_1101-2020_11_24_390039 80 15 insertion insertion NN 10_1101-2020_11_24_390039 80 16 of of IN 10_1101-2020_11_24_390039 80 17 any any DT 10_1101-2020_11_24_390039 80 18 of of IN 10_1101-2020_11_24_390039 80 19 our -PRON- PRP$ 10_1101-2020_11_24_390039 80 20 OPG2-tagged OPG2-tagged NNP 10_1101-2020_11_24_390039 80 21 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 80 22 variants variant NNS 10_1101-2020_11_24_390039 80 23 is be VBZ 10_1101-2020_11_24_390039 80 24 reduced reduce VBN 10_1101-2020_11_24_390039 80 25 by by IN 10_1101-2020_11_24_390039 80 26 Ipom Ipom NNP 10_1101-2020_11_24_390039 80 27 - - HYPH 10_1101-2020_11_24_390039 80 28 F F NNP 10_1101-2020_11_24_390039 80 29 , , , 10_1101-2020_11_24_390039 80 30 strongly strongly RB 10_1101-2020_11_24_390039 80 31 suggesting suggest VBG 10_1101-2020_11_24_390039 80 32 that that IN 10_1101-2020_11_24_390039 80 33 its -PRON- PRP$ 10_1101-2020_11_24_390039 80 34 association association NN 10_1101-2020_11_24_390039 80 35 with with IN 10_1101-2020_11_24_390039 80 36 the the DT 10_1101-2020_11_24_390039 80 37 inner inner JJ 10_1101-2020_11_24_390039 80 38 leaflet leaflet NN 10_1101-2020_11_24_390039 80 39 of of IN 10_1101-2020_11_24_390039 80 40 the the DT 10_1101-2020_11_24_390039 80 41 ER ER NNP 10_1101-2020_11_24_390039 80 42 membrane membrane NN 10_1101-2020_11_24_390039 80 43 does do VBZ 10_1101-2020_11_24_390039 80 44 not not RB 10_1101-2020_11_24_390039 80 45 require require VB 10_1101-2020_11_24_390039 80 46 protein protein NN 10_1101-2020_11_24_390039 80 47 translocation translocation NN 10_1101-2020_11_24_390039 80 48 via via IN 10_1101-2020_11_24_390039 80 49 the the DT 10_1101-2020_11_24_390039 80 50 central central JJ 10_1101-2020_11_24_390039 80 51 channel channel NN 10_1101-2020_11_24_390039 80 52 of of IN 10_1101-2020_11_24_390039 80 53 the the DT 10_1101-2020_11_24_390039 80 54 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 80 55 translocon translocon NN 10_1101-2020_11_24_390039 80 56 ( ( -LRB- 10_1101-2020_11_24_390039 80 57 Gérard Gérard NNP 10_1101-2020_11_24_390039 80 58 et et NNP 10_1101-2020_11_24_390039 80 59 al al NNP 10_1101-2020_11_24_390039 80 60 . . NNP 10_1101-2020_11_24_390039 80 61 , , , 10_1101-2020_11_24_390039 80 62 2020 2020 CD 10_1101-2020_11_24_390039 80 63 ; ; : 10_1101-2020_11_24_390039 80 64 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 80 65 et et NNP 10_1101-2020_11_24_390039 80 66 al al NNP 10_1101-2020_11_24_390039 80 67 . . NNP 10_1101-2020_11_24_390039 80 68 , , , 10_1101-2020_11_24_390039 80 69 2020 2020 CD 10_1101-2020_11_24_390039 80 70 submitted submit VBN 10_1101-2020_11_24_390039 80 71 ) ) -RRB- 10_1101-2020_11_24_390039 80 72 . . . 10_1101-2020_11_24_390039 81 1 We -PRON- PRP 10_1101-2020_11_24_390039 81 2 noted note VBD 10_1101-2020_11_24_390039 81 3 a a DT 10_1101-2020_11_24_390039 81 4 sub sub NN 10_1101-2020_11_24_390039 81 5 - - HYPH 10_1101-2020_11_24_390039 81 6 set set NN 10_1101-2020_11_24_390039 81 7 of of IN 10_1101-2020_11_24_390039 81 8 OPG2-ORF6-OPG2 OPG2-ORF6-OPG2 NNP 10_1101-2020_11_24_390039 81 9 species specie NNS 10_1101-2020_11_24_390039 81 10 bearing bear VBG 10_1101-2020_11_24_390039 81 11 only only RB 10_1101-2020_11_24_390039 81 12 a a DT 10_1101-2020_11_24_390039 81 13 single single JJ 10_1101-2020_11_24_390039 81 14 N N NNP 10_1101-2020_11_24_390039 81 15 - - HYPH 10_1101-2020_11_24_390039 81 16 glycan glycan NNP 10_1101-2020_11_24_390039 81 17 was be VBD 10_1101-2020_11_24_390039 81 18 also also RB 10_1101-2020_11_24_390039 81 19 clearly clearly RB 10_1101-2020_11_24_390039 81 20 present present JJ 10_1101-2020_11_24_390039 81 21 in in IN 10_1101-2020_11_24_390039 81 22 the the DT 10_1101-2020_11_24_390039 81 23 membrane membrane NN 10_1101-2020_11_24_390039 81 24 - - HYPH 10_1101-2020_11_24_390039 81 25 associated associate VBN 10_1101-2020_11_24_390039 81 26 fraction fraction NN 10_1101-2020_11_24_390039 81 27 with with IN 10_1101-2020_11_24_390039 81 28 or or CC 10_1101-2020_11_24_390039 81 29 without without IN 10_1101-2020_11_24_390039 81 30 Ipom Ipom NNP 10_1101-2020_11_24_390039 81 31 - - HYPH 10_1101-2020_11_24_390039 81 32 F F NNP 10_1101-2020_11_24_390039 81 33 treatment treatment NN 10_1101-2020_11_24_390039 81 34 ( ( -LRB- 10_1101-2020_11_24_390039 81 35 Fig fig NN 10_1101-2020_11_24_390039 81 36 . . . 10_1101-2020_11_24_390039 82 1 2B 2B NNP 10_1101-2020_11_24_390039 82 2 , , , 10_1101-2020_11_24_390039 82 3 OPG2-ORF6-OPG2 OPG2-ORF6-OPG2 NNP 10_1101-2020_11_24_390039 82 4 , , , 10_1101-2020_11_24_390039 82 5 see see VB 10_1101-2020_11_24_390039 82 6 1Gly 1gly CD 10_1101-2020_11_24_390039 82 7 ) ) -RRB- 10_1101-2020_11_24_390039 82 8 . . . 10_1101-2020_11_24_390039 83 1 Based base VBN 10_1101-2020_11_24_390039 83 2 on on IN 10_1101-2020_11_24_390039 83 3 comparison comparison NN 10_1101-2020_11_24_390039 83 4 to to TO 10_1101-2020_11_24_390039 83 5 singly singly RB 10_1101-2020_11_24_390039 83 6 OPG2-tagged opg2-tagged JJ 10_1101-2020_11_24_390039 83 7 variants variant NNS 10_1101-2020_11_24_390039 83 8 ( ( -LRB- 10_1101-2020_11_24_390039 83 9 Fig fig NN 10_1101-2020_11_24_390039 83 10 . . . 10_1101-2020_11_24_390039 84 1 2B 2B NNP 10_1101-2020_11_24_390039 84 2 ) ) -RRB- 10_1101-2020_11_24_390039 84 3 , , , 10_1101-2020_11_24_390039 84 4 we -PRON- PRP 10_1101-2020_11_24_390039 84 5 conclude conclude VBP 10_1101-2020_11_24_390039 84 6 that that IN 10_1101-2020_11_24_390039 84 7 OPG2-ORF6-OPG2- OPG2-ORF6-OPG2- NNP 10_1101-2020_11_24_390039 84 8 1Gly 1gly CD 10_1101-2020_11_24_390039 84 9 has have VBZ 10_1101-2020_11_24_390039 84 10 its -PRON- PRP$ 10_1101-2020_11_24_390039 84 11 N n NN 10_1101-2020_11_24_390039 84 12 - - HYPH 10_1101-2020_11_24_390039 84 13 terminus terminus NN 10_1101-2020_11_24_390039 84 14 in in IN 10_1101-2020_11_24_390039 84 15 the the DT 10_1101-2020_11_24_390039 84 16 ER ER NNP 10_1101-2020_11_24_390039 84 17 lumen luman NNS 10_1101-2020_11_24_390039 84 18 , , , 10_1101-2020_11_24_390039 84 19 where where WRB 10_1101-2020_11_24_390039 84 20 only only RB 10_1101-2020_11_24_390039 84 21 one one CD 10_1101-2020_11_24_390039 84 22 of of IN 10_1101-2020_11_24_390039 84 23 its -PRON- PRP$ 10_1101-2020_11_24_390039 84 24 two two CD 10_1101-2020_11_24_390039 84 25 consensus consensus NN 10_1101-2020_11_24_390039 84 26 sites site NNS 10_1101-2020_11_24_390039 84 27 is be VBZ 10_1101-2020_11_24_390039 84 28 efficiently efficiently RB 10_1101-2020_11_24_390039 84 29 N n NN 10_1101-2020_11_24_390039 84 30 - - HYPH 10_1101-2020_11_24_390039 84 31 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 84 32 ( ( -LRB- 10_1101-2020_11_24_390039 84 33 cf cf NN 10_1101-2020_11_24_390039 84 34 . . . 10_1101-2020_11_24_390039 85 1 Nilsson Nilsson NNP 10_1101-2020_11_24_390039 85 2 and and CC 10_1101-2020_11_24_390039 85 3 von von NNP 10_1101-2020_11_24_390039 85 4 Heijne Heijne NNP 10_1101-2020_11_24_390039 85 5 , , , 10_1101-2020_11_24_390039 85 6 1993 1993 CD 10_1101-2020_11_24_390039 85 7 ) ) -RRB- 10_1101-2020_11_24_390039 85 8 , , , 10_1101-2020_11_24_390039 85 9 whilst whilst IN 10_1101-2020_11_24_390039 85 10 its -PRON- PRP$ 10_1101-2020_11_24_390039 85 11 C- C- NNP 10_1101-2020_11_24_390039 85 12 terminus terminus NN 10_1101-2020_11_24_390039 85 13 is be VBZ 10_1101-2020_11_24_390039 85 14 either either CC 10_1101-2020_11_24_390039 85 15 ER ER NNP 10_1101-2020_11_24_390039 85 16 luminal luminal JJ 10_1101-2020_11_24_390039 85 17 but but CC 10_1101-2020_11_24_390039 85 18 non non JJ 10_1101-2020_11_24_390039 85 19 - - JJ 10_1101-2020_11_24_390039 85 20 glycosylated glycosylated JJ 10_1101-2020_11_24_390039 85 21 or or CC 10_1101-2020_11_24_390039 85 22 remains remain VBZ 10_1101-2020_11_24_390039 85 23 on on IN 10_1101-2020_11_24_390039 85 24 the the DT 10_1101-2020_11_24_390039 85 25 cytosolic cytosolic JJ 10_1101-2020_11_24_390039 85 26 side side NN 10_1101-2020_11_24_390039 85 27 of of IN 10_1101-2020_11_24_390039 85 28 the the DT 10_1101-2020_11_24_390039 85 29 membrane membrane NN 10_1101-2020_11_24_390039 85 30 . . . 10_1101-2020_11_24_390039 86 1 In in IN 10_1101-2020_11_24_390039 86 2 the the DT 10_1101-2020_11_24_390039 86 3 latter latter JJ 10_1101-2020_11_24_390039 86 4 case case NN 10_1101-2020_11_24_390039 86 5 , , , 10_1101-2020_11_24_390039 86 6 it -PRON- PRP 10_1101-2020_11_24_390039 86 7 may may MD 10_1101-2020_11_24_390039 86 8 be be VB 10_1101-2020_11_24_390039 86 9 that that IN 10_1101-2020_11_24_390039 86 10 , , , 10_1101-2020_11_24_390039 86 11 in in IN 10_1101-2020_11_24_390039 86 12 addition addition NN 10_1101-2020_11_24_390039 86 13 to to IN 10_1101-2020_11_24_390039 86 14 its -PRON- PRP$ 10_1101-2020_11_24_390039 86 15 hairpin hairpin NN 10_1101-2020_11_24_390039 86 16 topology topology NN 10_1101-2020_11_24_390039 86 17 , , , 10_1101-2020_11_24_390039 86 18 some some DT 10_1101-2020_11_24_390039 86 19 fraction fraction NN 10_1101-2020_11_24_390039 86 20 of of IN 10_1101-2020_11_24_390039 86 21 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 86 22 may may MD 10_1101-2020_11_24_390039 86 23 be be VB 10_1101-2020_11_24_390039 86 24 integrated integrate VBN 10_1101-2020_11_24_390039 86 25 into into IN 10_1101-2020_11_24_390039 86 26 ER ER NNP 10_1101-2020_11_24_390039 86 27 - - HYPH 10_1101-2020_11_24_390039 86 28 derived derive VBN 10_1101-2020_11_24_390039 86 29 microsomes microsome NNS 10_1101-2020_11_24_390039 86 30 as as IN 10_1101-2020_11_24_390039 86 31 a a DT 10_1101-2020_11_24_390039 86 32 type type NN 10_1101-2020_11_24_390039 86 33 III iii CD 10_1101-2020_11_24_390039 86 34 TMP TMP NNP 10_1101-2020_11_24_390039 86 35 ( ( -LRB- 10_1101-2020_11_24_390039 86 36 cf cf NN 10_1101-2020_11_24_390039 86 37 . . . 10_1101-2020_11_24_390039 87 1 Fig fig NN 10_1101-2020_11_24_390039 87 2 . . . 10_1101-2020_11_24_390039 88 1 S2E s2e LS 10_1101-2020_11_24_390039 88 2 ; ; : 10_1101-2020_11_24_390039 88 3 see see VB 10_1101-2020_11_24_390039 88 4 also also RB 10_1101-2020_11_24_390039 88 5 Netland Netland NNP 10_1101-2020_11_24_390039 88 6 et et FW 10_1101-2020_11_24_390039 88 7 al al NNP 10_1101-2020_11_24_390039 88 8 . . NNP 10_1101-2020_11_24_390039 88 9 , , , 10_1101-2020_11_24_390039 88 10 2007 2007 CD 10_1101-2020_11_24_390039 88 11 ) ) -RRB- 10_1101-2020_11_24_390039 88 12 that that WDT 10_1101-2020_11_24_390039 88 13 is be VBZ 10_1101-2020_11_24_390039 88 14 resistant resistant JJ 10_1101-2020_11_24_390039 88 15 to to IN 10_1101-2020_11_24_390039 88 16 Ipom Ipom NNP 10_1101-2020_11_24_390039 88 17 - - HYPH 10_1101-2020_11_24_390039 88 18 F F NNP 10_1101-2020_11_24_390039 88 19 inhibition inhibition NN 10_1101-2020_11_24_390039 88 20 ( ( -LRB- 10_1101-2020_11_24_390039 88 21 this this DT 10_1101-2020_11_24_390039 88 22 study study NN 10_1101-2020_11_24_390039 88 23 ; ; : 10_1101-2020_11_24_390039 88 24 Zong Zong NNP 10_1101-2020_11_24_390039 88 25 et et NNP 10_1101-2020_11_24_390039 88 26 al al NNP 10_1101-2020_11_24_390039 88 27 . . NNP 10_1101-2020_11_24_390039 88 28 , , , 10_1101-2020_11_24_390039 88 29 2019 2019 CD 10_1101-2020_11_24_390039 88 30 ; ; : 10_1101-2020_11_24_390039 88 31 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 88 32 et et NNP 10_1101-2020_11_24_390039 88 33 al al NNP 10_1101-2020_11_24_390039 88 34 . . NNP 10_1101-2020_11_24_390039 88 35 , , , 10_1101-2020_11_24_390039 88 36 2020 2020 CD 10_1101-2020_11_24_390039 88 37 submitted submit VBN 10_1101-2020_11_24_390039 88 38 ) ) -RRB- 10_1101-2020_11_24_390039 88 39 . . . 10_1101-2020_11_24_390039 89 1 .CC .CC NFP 10_1101-2020_11_24_390039 89 2 - - : 10_1101-2020_11_24_390039 89 3 BY by IN 10_1101-2020_11_24_390039 89 4 - - HYPH 10_1101-2020_11_24_390039 89 5 ND ND NNP 10_1101-2020_11_24_390039 89 6 4.0 4.0 CD 10_1101-2020_11_24_390039 89 7 International international JJ 10_1101-2020_11_24_390039 89 8 licensemade licensemade NN 10_1101-2020_11_24_390039 89 9 available available JJ 10_1101-2020_11_24_390039 89 10 under under IN 10_1101-2020_11_24_390039 89 11 a a DT 10_1101-2020_11_24_390039 89 12 ( ( -LRB- 10_1101-2020_11_24_390039 89 13 which which WDT 10_1101-2020_11_24_390039 89 14 was be VBD 10_1101-2020_11_24_390039 89 15 not not RB 10_1101-2020_11_24_390039 89 16 certified certify VBN 10_1101-2020_11_24_390039 89 17 by by IN 10_1101-2020_11_24_390039 89 18 peer peer NN 10_1101-2020_11_24_390039 89 19 review review NN 10_1101-2020_11_24_390039 89 20 ) ) -RRB- 10_1101-2020_11_24_390039 89 21 is be VBZ 10_1101-2020_11_24_390039 89 22 the the DT 10_1101-2020_11_24_390039 89 23 author author NN 10_1101-2020_11_24_390039 89 24 / / SYM 10_1101-2020_11_24_390039 89 25 funder funder NN 10_1101-2020_11_24_390039 89 26 , , , 10_1101-2020_11_24_390039 89 27 who who WP 10_1101-2020_11_24_390039 89 28 has have VBZ 10_1101-2020_11_24_390039 89 29 granted grant VBN 10_1101-2020_11_24_390039 89 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 89 31 a a DT 10_1101-2020_11_24_390039 89 32 license license NN 10_1101-2020_11_24_390039 89 33 to to TO 10_1101-2020_11_24_390039 89 34 display display VB 10_1101-2020_11_24_390039 89 35 the the DT 10_1101-2020_11_24_390039 89 36 preprint preprint NN 10_1101-2020_11_24_390039 89 37 in in IN 10_1101-2020_11_24_390039 89 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 89 39 . . . 10_1101-2020_11_24_390039 90 1 It -PRON- PRP 10_1101-2020_11_24_390039 90 2 is be VBZ 10_1101-2020_11_24_390039 90 3 The the DT 10_1101-2020_11_24_390039 90 4 copyright copyright NN 10_1101-2020_11_24_390039 90 5 holder holder NN 10_1101-2020_11_24_390039 90 6 for for IN 10_1101-2020_11_24_390039 90 7 this this DT 10_1101-2020_11_24_390039 90 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 90 9 version version NN 10_1101-2020_11_24_390039 90 10 posted post VBD 10_1101-2020_11_24_390039 90 11 January January NNP 10_1101-2020_11_24_390039 90 12 5 5 CD 10_1101-2020_11_24_390039 90 13 , , , 10_1101-2020_11_24_390039 90 14 2021 2021 CD 10_1101-2020_11_24_390039 90 15 . . . 10_1101-2020_11_24_390039 90 16 ; ; : 10_1101-2020_11_24_390039 90 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 90 18 : : : 10_1101-2020_11_24_390039 90 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 90 20 preprint preprint JJ 10_1101-2020_11_24_390039 90 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 90 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 90 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 90 24 - - HYPH 10_1101-2020_11_24_390039 90 25 F F NNP 10_1101-2020_11_24_390039 90 26 as as IN 10_1101-2020_11_24_390039 90 27 a a DT 10_1101-2020_11_24_390039 90 28 potential potential JJ 10_1101-2020_11_24_390039 90 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 90 30 agent agent NN 10_1101-2020_11_24_390039 90 31 Page Page NNP 10_1101-2020_11_24_390039 90 32 7 7 CD 10_1101-2020_11_24_390039 90 33 of of IN 10_1101-2020_11_24_390039 90 34 21 21 CD 10_1101-2020_11_24_390039 90 35 The the DT 10_1101-2020_11_24_390039 90 36 molecular molecular JJ 10_1101-2020_11_24_390039 90 37 basis basis NN 10_1101-2020_11_24_390039 90 38 for for IN 10_1101-2020_11_24_390039 90 39 SARS SARS NNP 10_1101-2020_11_24_390039 90 40 - - HYPH 10_1101-2020_11_24_390039 90 41 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 90 42 protein protein NN 10_1101-2020_11_24_390039 90 43 sensitivity sensitivity NN 10_1101-2020_11_24_390039 90 44 to to IN 10_1101-2020_11_24_390039 90 45 Ipom Ipom NNP 10_1101-2020_11_24_390039 90 46 - - HYPH 10_1101-2020_11_24_390039 90 47 F F NNP 10_1101-2020_11_24_390039 90 48 Having have VBG 10_1101-2020_11_24_390039 90 49 ascertained ascertain VBN 10_1101-2020_11_24_390039 90 50 that that IN 10_1101-2020_11_24_390039 90 51 Ipom Ipom NNP 10_1101-2020_11_24_390039 90 52 - - HYPH 10_1101-2020_11_24_390039 90 53 F F NNP 10_1101-2020_11_24_390039 90 54 inhibits inhibit VBZ 10_1101-2020_11_24_390039 90 55 the the DT 10_1101-2020_11_24_390039 90 56 ER ER NNP 10_1101-2020_11_24_390039 90 57 membrane membrane NN 10_1101-2020_11_24_390039 90 58 translocation translocation NN 10_1101-2020_11_24_390039 90 59 / / SYM 10_1101-2020_11_24_390039 90 60 insertion insertion NN 10_1101-2020_11_24_390039 90 61 of of IN 10_1101-2020_11_24_390039 90 62 the the DT 10_1101-2020_11_24_390039 90 63 viral viral JJ 10_1101-2020_11_24_390039 90 64 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 90 65 and and CC 10_1101-2020_11_24_390039 90 66 S S NNP 10_1101-2020_11_24_390039 90 67 proteins protein NNS 10_1101-2020_11_24_390039 90 68 , , , 10_1101-2020_11_24_390039 90 69 but but CC 10_1101-2020_11_24_390039 90 70 not not RB 10_1101-2020_11_24_390039 90 71 of of IN 10_1101-2020_11_24_390039 90 72 the the DT 10_1101-2020_11_24_390039 90 73 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 90 74 , , , 10_1101-2020_11_24_390039 90 75 E E NNP 10_1101-2020_11_24_390039 90 76 or or CC 10_1101-2020_11_24_390039 90 77 M M NNP 10_1101-2020_11_24_390039 90 78 proteins protein NNS 10_1101-2020_11_24_390039 90 79 ( ( -LRB- 10_1101-2020_11_24_390039 90 80 Fig fig NN 10_1101-2020_11_24_390039 90 81 . . . 10_1101-2020_11_24_390039 91 1 2C 2C NNP 10_1101-2020_11_24_390039 91 2 ) ) -RRB- 10_1101-2020_11_24_390039 91 3 , , , 10_1101-2020_11_24_390039 91 4 we -PRON- PRP 10_1101-2020_11_24_390039 91 5 next next RB 10_1101-2020_11_24_390039 91 6 investigated investigate VBD 10_1101-2020_11_24_390039 91 7 the the DT 10_1101-2020_11_24_390039 91 8 molecular molecular JJ 10_1101-2020_11_24_390039 91 9 basis basis NN 10_1101-2020_11_24_390039 91 10 for for IN 10_1101-2020_11_24_390039 91 11 this this DT 10_1101-2020_11_24_390039 91 12 selectivity selectivity NN 10_1101-2020_11_24_390039 91 13 . . . 10_1101-2020_11_24_390039 92 1 For for IN 10_1101-2020_11_24_390039 92 2 these these DT 10_1101-2020_11_24_390039 92 3 studies study NNS 10_1101-2020_11_24_390039 92 4 we -PRON- PRP 10_1101-2020_11_24_390039 92 5 employed employ VBD 10_1101-2020_11_24_390039 92 6 semi semi RB 10_1101-2020_11_24_390039 92 7 - - JJ 10_1101-2020_11_24_390039 92 8 permeabilised permeabilised JJ 10_1101-2020_11_24_390039 92 9 ( ( -LRB- 10_1101-2020_11_24_390039 92 10 SP SP NNP 10_1101-2020_11_24_390039 92 11 ) ) -RRB- 10_1101-2020_11_24_390039 92 12 mammalian mammalian JJ 10_1101-2020_11_24_390039 92 13 cells cell NNS 10_1101-2020_11_24_390039 92 14 , , , 10_1101-2020_11_24_390039 92 15 depleted deplete VBN 10_1101-2020_11_24_390039 92 16 of of IN 10_1101-2020_11_24_390039 92 17 specific specific JJ 10_1101-2020_11_24_390039 92 18 membrane membrane NN 10_1101-2020_11_24_390039 92 19 components component NNS 10_1101-2020_11_24_390039 92 20 via via IN 10_1101-2020_11_24_390039 92 21 siRNA sirna NN 10_1101-2020_11_24_390039 92 22 - - HYPH 10_1101-2020_11_24_390039 92 23 mediated mediate VBN 10_1101-2020_11_24_390039 92 24 knockdown knockdown VBN 10_1101-2020_11_24_390039 92 25 , , , 10_1101-2020_11_24_390039 92 26 as as IN 10_1101-2020_11_24_390039 92 27 our -PRON- PRP$ 10_1101-2020_11_24_390039 92 28 source source NN 10_1101-2020_11_24_390039 92 29 of of IN 10_1101-2020_11_24_390039 92 30 ER ER NNP 10_1101-2020_11_24_390039 92 31 membrane membrane NN 10_1101-2020_11_24_390039 92 32 ( ( -LRB- 10_1101-2020_11_24_390039 92 33 Fig fig NN 10_1101-2020_11_24_390039 92 34 . . . 10_1101-2020_11_24_390039 93 1 3A 3A NNP 10_1101-2020_11_24_390039 93 2 ; ; : 10_1101-2020_11_24_390039 93 3 Wilson Wilson NNP 10_1101-2020_11_24_390039 93 4 et et NNP 10_1101-2020_11_24_390039 93 5 al al NNP 10_1101-2020_11_24_390039 93 6 . . NNP 10_1101-2020_11_24_390039 93 7 , , , 10_1101-2020_11_24_390039 93 8 2007 2007 CD 10_1101-2020_11_24_390039 93 9 ) ) -RRB- 10_1101-2020_11_24_390039 93 10 . . . 10_1101-2020_11_24_390039 94 1 Consistent consistent JJ 10_1101-2020_11_24_390039 94 2 with with IN 10_1101-2020_11_24_390039 94 3 our -PRON- PRP$ 10_1101-2020_11_24_390039 94 4 recent recent JJ 10_1101-2020_11_24_390039 94 5 work work NN 10_1101-2020_11_24_390039 94 6 ( ( -LRB- 10_1101-2020_11_24_390039 94 7 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 94 8 et et NNP 10_1101-2020_11_24_390039 94 9 al al NNP 10_1101-2020_11_24_390039 94 10 . . NNP 10_1101-2020_11_24_390039 94 11 , , , 10_1101-2020_11_24_390039 94 12 2020 2020 CD 10_1101-2020_11_24_390039 94 13 submitted submit VBN 10_1101-2020_11_24_390039 94 14 ) ) -RRB- 10_1101-2020_11_24_390039 94 15 , , , 10_1101-2020_11_24_390039 94 16 and and CC 10_1101-2020_11_24_390039 94 17 based base VBN 10_1101-2020_11_24_390039 94 18 on on IN 10_1101-2020_11_24_390039 94 19 the the DT 10_1101-2020_11_24_390039 94 20 quantitative quantitative JJ 10_1101-2020_11_24_390039 94 21 immunoblotting immunoblotting NN 10_1101-2020_11_24_390039 94 22 of of IN 10_1101-2020_11_24_390039 94 23 target target NN 10_1101-2020_11_24_390039 94 24 and and CC 10_1101-2020_11_24_390039 94 25 control control NN 10_1101-2020_11_24_390039 94 26 gene gene NN 10_1101-2020_11_24_390039 94 27 products product NNS 10_1101-2020_11_24_390039 94 28 ( ( -LRB- 10_1101-2020_11_24_390039 94 29 Fig fig NN 10_1101-2020_11_24_390039 94 30 . . . 10_1101-2020_11_24_390039 95 1 S2A S2A NNP 10_1101-2020_11_24_390039 95 2 - - HYPH 10_1101-2020_11_24_390039 95 3 C C NNP 10_1101-2020_11_24_390039 95 4 ) ) -RRB- 10_1101-2020_11_24_390039 95 5 , , , 10_1101-2020_11_24_390039 95 6 we -PRON- PRP 10_1101-2020_11_24_390039 95 7 selectively selectively RB 10_1101-2020_11_24_390039 95 8 depleted deplete VBD 10_1101-2020_11_24_390039 95 9 HeLa HeLa NNP 10_1101-2020_11_24_390039 95 10 cells cell NNS 10_1101-2020_11_24_390039 95 11 for for IN 10_1101-2020_11_24_390039 95 12 core core JJ 10_1101-2020_11_24_390039 95 13 components component NNS 10_1101-2020_11_24_390039 95 14 of of IN 10_1101-2020_11_24_390039 95 15 the the DT 10_1101-2020_11_24_390039 95 16 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 95 17 translocon translocon NN 10_1101-2020_11_24_390039 95 18 ( ( -LRB- 10_1101-2020_11_24_390039 95 19 Sec61α Sec61α NNP 10_1101-2020_11_24_390039 95 20 - - HYPH 10_1101-2020_11_24_390039 95 21 kd kd NNP 10_1101-2020_11_24_390039 95 22 , , , 10_1101-2020_11_24_390039 95 23 ~65 ~65 NFP 10_1101-2020_11_24_390039 95 24 % % NN 10_1101-2020_11_24_390039 95 25 reduction reduction NN 10_1101-2020_11_24_390039 95 26 ) ) -RRB- 10_1101-2020_11_24_390039 95 27 , , , 10_1101-2020_11_24_390039 95 28 the the DT 10_1101-2020_11_24_390039 95 29 EMC EMC NNP 10_1101-2020_11_24_390039 95 30 ( ( -LRB- 10_1101-2020_11_24_390039 95 31 EMC5-kd EMC5-kd NNP 10_1101-2020_11_24_390039 95 32 , , , 10_1101-2020_11_24_390039 95 33 ~73 ~73 . 10_1101-2020_11_24_390039 95 34 % % NN 10_1101-2020_11_24_390039 95 35 reduction reduction NN 10_1101-2020_11_24_390039 95 36 ) ) -RRB- 10_1101-2020_11_24_390039 95 37 and and CC 10_1101-2020_11_24_390039 95 38 both both CC 10_1101-2020_11_24_390039 95 39 together together RB 10_1101-2020_11_24_390039 95 40 ( ( -LRB- 10_1101-2020_11_24_390039 95 41 Sec61α+EMC5-kd Sec61α+EMC5-kd NNS 10_1101-2020_11_24_390039 95 42 , , , 10_1101-2020_11_24_390039 95 43 ~68 ~68 . 10_1101-2020_11_24_390039 95 44 % % NN 10_1101-2020_11_24_390039 95 45 and and CC 10_1101-2020_11_24_390039 95 46 ~78 ~78 NFP 10_1101-2020_11_24_390039 95 47 % % NN 10_1101-2020_11_24_390039 95 48 reduction reduction NNP 10_1101-2020_11_24_390039 95 49 ) ) -RRB- 10_1101-2020_11_24_390039 95 50 prior prior RB 10_1101-2020_11_24_390039 95 51 to to IN 10_1101-2020_11_24_390039 95 52 semi semi JJ 10_1101-2020_11_24_390039 95 53 - - NN 10_1101-2020_11_24_390039 95 54 permeabilisation permeabilisation NN 10_1101-2020_11_24_390039 95 55 with with IN 10_1101-2020_11_24_390039 95 56 digitonin digitonin NN 10_1101-2020_11_24_390039 95 57 and and CC 10_1101-2020_11_24_390039 95 58 use use NN 10_1101-2020_11_24_390039 95 59 for for IN 10_1101-2020_11_24_390039 95 60 in in FW 10_1101-2020_11_24_390039 95 61 vitro vitro FW 10_1101-2020_11_24_390039 95 62 ER ER NNP 10_1101-2020_11_24_390039 95 63 translocation translocation NN 10_1101-2020_11_24_390039 95 64 assays assays RB 10_1101-2020_11_24_390039 95 65 . . . 10_1101-2020_11_24_390039 96 1 Following follow VBG 10_1101-2020_11_24_390039 96 2 the the DT 10_1101-2020_11_24_390039 96 3 analysis analysis NN 10_1101-2020_11_24_390039 96 4 of of IN 10_1101-2020_11_24_390039 96 5 total total JJ 10_1101-2020_11_24_390039 96 6 OPG2-tagged opg2-tagged JJ 10_1101-2020_11_24_390039 96 7 translation translation NN 10_1101-2020_11_24_390039 96 8 products product NNS 10_1101-2020_11_24_390039 96 9 recovered recover VBN 10_1101-2020_11_24_390039 96 10 by by IN 10_1101-2020_11_24_390039 96 11 immunoprecipitation immunoprecipitation NN 10_1101-2020_11_24_390039 96 12 , , , 10_1101-2020_11_24_390039 96 13 we -PRON- PRP 10_1101-2020_11_24_390039 96 14 found find VBD 10_1101-2020_11_24_390039 96 15 that that DT 10_1101-2020_11_24_390039 96 16 : : : 10_1101-2020_11_24_390039 96 17 i i PRP 10_1101-2020_11_24_390039 96 18 ) ) -RRB- 10_1101-2020_11_24_390039 96 19 the the DT 10_1101-2020_11_24_390039 96 20 S S NNP 10_1101-2020_11_24_390039 96 21 protein protein NN 10_1101-2020_11_24_390039 96 22 and and CC 10_1101-2020_11_24_390039 96 23 a a DT 10_1101-2020_11_24_390039 96 24 truncated truncate VBN 10_1101-2020_11_24_390039 96 25 derivative derivative NN 10_1101-2020_11_24_390039 96 26 were be VBD 10_1101-2020_11_24_390039 96 27 both both DT 10_1101-2020_11_24_390039 96 28 more more RBR 10_1101-2020_11_24_390039 96 29 strongly strongly RB 10_1101-2020_11_24_390039 96 30 affected affect VBN 10_1101-2020_11_24_390039 96 31 by by IN 10_1101-2020_11_24_390039 96 32 the the DT 10_1101-2020_11_24_390039 96 33 depletion depletion NN 10_1101-2020_11_24_390039 96 34 of of IN 10_1101-2020_11_24_390039 96 35 Sec61α Sec61α NNP 10_1101-2020_11_24_390039 96 36 than than IN 10_1101-2020_11_24_390039 96 37 of of IN 10_1101-2020_11_24_390039 96 38 EMC5 EMC5 NNP 10_1101-2020_11_24_390039 96 39 ( ( -LRB- 10_1101-2020_11_24_390039 96 40 Fig Fig NNP 10_1101-2020_11_24_390039 96 41 . . . 10_1101-2020_11_24_390039 97 1 3B 3B NNP 10_1101-2020_11_24_390039 97 2 , , , 10_1101-2020_11_24_390039 97 3 Fig Fig NNP 10_1101-2020_11_24_390039 97 4 . . . 10_1101-2020_11_24_390039 98 1 S2D s2d LS 10_1101-2020_11_24_390039 98 2 ) ) -RRB- 10_1101-2020_11_24_390039 98 3 ; ; : 10_1101-2020_11_24_390039 98 4 ii ii LS 10_1101-2020_11_24_390039 98 5 ) ) -RRB- 10_1101-2020_11_24_390039 98 6 the the DT 10_1101-2020_11_24_390039 98 7 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 98 8 protein protein NN 10_1101-2020_11_24_390039 98 9 was be VBD 10_1101-2020_11_24_390039 98 10 likewise likewise RB 10_1101-2020_11_24_390039 98 11 strongly strongly RB 10_1101-2020_11_24_390039 98 12 affected affect VBN 10_1101-2020_11_24_390039 98 13 by by IN 10_1101-2020_11_24_390039 98 14 Sec61α sec61α CD 10_1101-2020_11_24_390039 98 15 depletion depletion NN 10_1101-2020_11_24_390039 98 16 but but CC 10_1101-2020_11_24_390039 98 17 also also RB 10_1101-2020_11_24_390039 98 18 sensitive sensitive JJ 10_1101-2020_11_24_390039 98 19 to to IN 10_1101-2020_11_24_390039 98 20 EMC5 EMC5 NNP 10_1101-2020_11_24_390039 98 21 depletion depletion NN 10_1101-2020_11_24_390039 98 22 ( ( -LRB- 10_1101-2020_11_24_390039 98 23 Fig fig NN 10_1101-2020_11_24_390039 98 24 . . . 10_1101-2020_11_24_390039 99 1 3C 3c NN 10_1101-2020_11_24_390039 99 2 ) ) -RRB- 10_1101-2020_11_24_390039 99 3 ; ; : 10_1101-2020_11_24_390039 99 4 iii iii LS 10_1101-2020_11_24_390039 99 5 ) ) -RRB- 10_1101-2020_11_24_390039 99 6 the the DT 10_1101-2020_11_24_390039 99 7 E E NNP 10_1101-2020_11_24_390039 99 8 protein protein NN 10_1101-2020_11_24_390039 99 9 showed show VBD 10_1101-2020_11_24_390039 99 10 diminished diminished JJ 10_1101-2020_11_24_390039 99 11 insertion insertion NN 10_1101-2020_11_24_390039 99 12 efficiency efficiency NN 10_1101-2020_11_24_390039 99 13 after after IN 10_1101-2020_11_24_390039 99 14 knock knock NN 10_1101-2020_11_24_390039 99 15 - - HYPH 10_1101-2020_11_24_390039 99 16 down down NN 10_1101-2020_11_24_390039 99 17 of of IN 10_1101-2020_11_24_390039 99 18 Sec61α Sec61α NNP 10_1101-2020_11_24_390039 99 19 and and CC 10_1101-2020_11_24_390039 99 20 EMC5 EMC5 NNP 10_1101-2020_11_24_390039 99 21 , , , 10_1101-2020_11_24_390039 99 22 although although IN 10_1101-2020_11_24_390039 99 23 the the DT 10_1101-2020_11_24_390039 99 24 latter latter NN 10_1101-2020_11_24_390039 99 25 had have VBD 10_1101-2020_11_24_390039 99 26 a a DT 10_1101-2020_11_24_390039 99 27 more more RBR 10_1101-2020_11_24_390039 99 28 pronounced pronounced JJ 10_1101-2020_11_24_390039 99 29 effect effect NN 10_1101-2020_11_24_390039 99 30 ( ( -LRB- 10_1101-2020_11_24_390039 99 31 Fig fig NN 10_1101-2020_11_24_390039 99 32 . . . 10_1101-2020_11_24_390039 100 1 3D 3d NN 10_1101-2020_11_24_390039 100 2 ) ) -RRB- 10_1101-2020_11_24_390039 100 3 . . . 10_1101-2020_11_24_390039 101 1 In in IN 10_1101-2020_11_24_390039 101 2 each each DT 10_1101-2020_11_24_390039 101 3 case case NN 10_1101-2020_11_24_390039 101 4 , , , 10_1101-2020_11_24_390039 101 5 the the DT 10_1101-2020_11_24_390039 101 6 combined combine VBN 10_1101-2020_11_24_390039 101 7 knockdown knockdown NNP 10_1101-2020_11_24_390039 101 8 of of IN 10_1101-2020_11_24_390039 101 9 Sec61α Sec61α NNP 10_1101-2020_11_24_390039 101 10 and and CC 10_1101-2020_11_24_390039 101 11 EMC5 EMC5 NNP 10_1101-2020_11_24_390039 101 12 resulted result VBD 10_1101-2020_11_24_390039 101 13 in in IN 10_1101-2020_11_24_390039 101 14 a a DT 10_1101-2020_11_24_390039 101 15 reduction reduction NN 10_1101-2020_11_24_390039 101 16 of of IN 10_1101-2020_11_24_390039 101 17 membrane membrane NN 10_1101-2020_11_24_390039 101 18 insertion insertion NN 10_1101-2020_11_24_390039 101 19 that that WDT 10_1101-2020_11_24_390039 101 20 was be VBD 10_1101-2020_11_24_390039 101 21 either either CC 10_1101-2020_11_24_390039 101 22 comparable comparable JJ 10_1101-2020_11_24_390039 101 23 to to IN 10_1101-2020_11_24_390039 101 24 , , , 10_1101-2020_11_24_390039 101 25 or or CC 10_1101-2020_11_24_390039 101 26 greater great JJR 10_1101-2020_11_24_390039 101 27 than than IN 10_1101-2020_11_24_390039 101 28 , , , 10_1101-2020_11_24_390039 101 29 that that DT 10_1101-2020_11_24_390039 101 30 achieved achieve VBD 10_1101-2020_11_24_390039 101 31 following follow VBG 10_1101-2020_11_24_390039 101 32 the the DT 10_1101-2020_11_24_390039 101 33 knock- knock- NN 10_1101-2020_11_24_390039 101 34 down down IN 10_1101-2020_11_24_390039 101 35 of of IN 10_1101-2020_11_24_390039 101 36 Sec61α Sec61α NNP 10_1101-2020_11_24_390039 101 37 alone alone RB 10_1101-2020_11_24_390039 101 38 ( ( -LRB- 10_1101-2020_11_24_390039 101 39 Figs fig NNS 10_1101-2020_11_24_390039 101 40 . . . 10_1101-2020_11_24_390039 102 1 3B 3b JJ 10_1101-2020_11_24_390039 102 2 to to IN 10_1101-2020_11_24_390039 102 3 3D 3d NN 10_1101-2020_11_24_390039 102 4 ) ) -RRB- 10_1101-2020_11_24_390039 102 5 . . . 10_1101-2020_11_24_390039 103 1 For for IN 10_1101-2020_11_24_390039 103 2 the the DT 10_1101-2020_11_24_390039 103 3 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 103 4 protein protein NN 10_1101-2020_11_24_390039 103 5 , , , 10_1101-2020_11_24_390039 103 6 the the DT 10_1101-2020_11_24_390039 103 7 total total JJ 10_1101-2020_11_24_390039 103 8 level level NN 10_1101-2020_11_24_390039 103 9 of of IN 10_1101-2020_11_24_390039 103 10 N n NN 10_1101-2020_11_24_390039 103 11 - - HYPH 10_1101-2020_11_24_390039 103 12 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 103 13 OPG2-ORF6-OPG2 OPG2-ORF6-OPG2 NNP 10_1101-2020_11_24_390039 103 14 species specie NNS 10_1101-2020_11_24_390039 103 15 was be VBD 10_1101-2020_11_24_390039 103 16 unaffected unaffected JJ 10_1101-2020_11_24_390039 103 17 by by IN 10_1101-2020_11_24_390039 103 18 any any DT 10_1101-2020_11_24_390039 103 19 knockdown knockdown VBN 10_1101-2020_11_24_390039 103 20 condition condition NN 10_1101-2020_11_24_390039 103 21 tested test VBN 10_1101-2020_11_24_390039 103 22 ( ( -LRB- 10_1101-2020_11_24_390039 103 23 Fig fig NN 10_1101-2020_11_24_390039 103 24 . . . 10_1101-2020_11_24_390039 104 1 3E 3E NNP 10_1101-2020_11_24_390039 104 2 ) ) -RRB- 10_1101-2020_11_24_390039 104 3 . . . 10_1101-2020_11_24_390039 105 1 However however RB 10_1101-2020_11_24_390039 105 2 , , , 10_1101-2020_11_24_390039 105 3 we -PRON- PRP 10_1101-2020_11_24_390039 105 4 note note VBP 10_1101-2020_11_24_390039 105 5 a a DT 10_1101-2020_11_24_390039 105 6 marked marked JJ 10_1101-2020_11_24_390039 105 7 increase increase NN 10_1101-2020_11_24_390039 105 8 in in IN 10_1101-2020_11_24_390039 105 9 the the DT 10_1101-2020_11_24_390039 105 10 proportion proportion NN 10_1101-2020_11_24_390039 105 11 of of IN 10_1101-2020_11_24_390039 105 12 potentially potentially RB 10_1101-2020_11_24_390039 105 13 mis mis NN 10_1101-2020_11_24_390039 105 14 - - HYPH 10_1101-2020_11_24_390039 105 15 inserted insert VBN 10_1101-2020_11_24_390039 105 16 OPG2-ORF6-OPG2 opg2-orf6-opg2 CD 10_1101-2020_11_24_390039 105 17 - - HYPH 10_1101-2020_11_24_390039 105 18 1Gly 1Gly NNS 10_1101-2020_11_24_390039 105 19 species specie NNS 10_1101-2020_11_24_390039 105 20 , , , 10_1101-2020_11_24_390039 105 21 particularly particularly RB 10_1101-2020_11_24_390039 105 22 after after IN 10_1101-2020_11_24_390039 105 23 co co NN 10_1101-2020_11_24_390039 105 24 - - NN 10_1101-2020_11_24_390039 105 25 depletion depletion NN 10_1101-2020_11_24_390039 105 26 of of IN 10_1101-2020_11_24_390039 105 27 EMC5 EMC5 NNP 10_1101-2020_11_24_390039 105 28 and and CC 10_1101-2020_11_24_390039 105 29 Sec61α Sec61α NNP 10_1101-2020_11_24_390039 105 30 ( ( -LRB- 10_1101-2020_11_24_390039 105 31 see see VB 10_1101-2020_11_24_390039 105 32 Fig Fig NNP 10_1101-2020_11_24_390039 105 33 . . . 10_1101-2020_11_24_390039 106 1 3E 3e NN 10_1101-2020_11_24_390039 106 2 ; ; : 10_1101-2020_11_24_390039 106 3 Fig Fig NNP 10_1101-2020_11_24_390039 106 4 . . . 10_1101-2020_11_24_390039 107 1 S2E s2e LS 10_1101-2020_11_24_390039 107 2 ) ) -RRB- 10_1101-2020_11_24_390039 107 3 . . . 10_1101-2020_11_24_390039 108 1 We -PRON- PRP 10_1101-2020_11_24_390039 108 2 speculate speculate VBP 10_1101-2020_11_24_390039 108 3 that that IN 10_1101-2020_11_24_390039 108 4 the the DT 10_1101-2020_11_24_390039 108 5 unusual unusual JJ 10_1101-2020_11_24_390039 108 6 hairpin hairpin NN 10_1101-2020_11_24_390039 108 7 topology topology NN 10_1101-2020_11_24_390039 108 8 of of IN 10_1101-2020_11_24_390039 108 9 the the DT 10_1101-2020_11_24_390039 108 10 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 108 11 protein protein NN 10_1101-2020_11_24_390039 108 12 may may MD 10_1101-2020_11_24_390039 108 13 be be VB 10_1101-2020_11_24_390039 108 14 attributed attribute VBN 10_1101-2020_11_24_390039 108 15 to to IN 10_1101-2020_11_24_390039 108 16 the the DT 10_1101-2020_11_24_390039 108 17 EMC EMC NNP 10_1101-2020_11_24_390039 108 18 and and CC 10_1101-2020_11_24_390039 108 19 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 108 20 complex complex JJ 10_1101-2020_11_24_390039 108 21 acting acting NN 10_1101-2020_11_24_390039 108 22 in in IN 10_1101-2020_11_24_390039 108 23 concert concert NN 10_1101-2020_11_24_390039 108 24 to to TO 10_1101-2020_11_24_390039 108 25 provide provide VB 10_1101-2020_11_24_390039 108 26 an an DT 10_1101-2020_11_24_390039 108 27 Ipom Ipom NNP 10_1101-2020_11_24_390039 108 28 - - HYPH 10_1101-2020_11_24_390039 108 29 F F NNP 10_1101-2020_11_24_390039 108 30 insensitive insensitive JJ 10_1101-2020_11_24_390039 108 31 pathway pathway NN 10_1101-2020_11_24_390039 108 32 for for IN 10_1101-2020_11_24_390039 108 33 protein protein NN 10_1101-2020_11_24_390039 108 34 translocation translocation NN 10_1101-2020_11_24_390039 108 35 across across IN 10_1101-2020_11_24_390039 108 36 the the DT 10_1101-2020_11_24_390039 108 37 ER ER NNP 10_1101-2020_11_24_390039 108 38 membrane membrane NN 10_1101-2020_11_24_390039 108 39 ( ( -LRB- 10_1101-2020_11_24_390039 108 40 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 108 41 et et NNP 10_1101-2020_11_24_390039 108 42 al al NNP 10_1101-2020_11_24_390039 108 43 . . NNP 10_1101-2020_11_24_390039 108 44 , , , 10_1101-2020_11_24_390039 108 45 2020 2020 CD 10_1101-2020_11_24_390039 108 46 , , , 10_1101-2020_11_24_390039 108 47 submitted submit VBN 10_1101-2020_11_24_390039 108 48 ) ) -RRB- 10_1101-2020_11_24_390039 108 49 . . . 10_1101-2020_11_24_390039 109 1 Perturbation perturbation NN 10_1101-2020_11_24_390039 109 2 of of IN 10_1101-2020_11_24_390039 109 3 this this DT 10_1101-2020_11_24_390039 109 4 pathway pathway NN 10_1101-2020_11_24_390039 109 5 seemingly seemingly RB 10_1101-2020_11_24_390039 109 6 increases increase VBZ 10_1101-2020_11_24_390039 109 7 the the DT 10_1101-2020_11_24_390039 109 8 potential potential NN 10_1101-2020_11_24_390039 109 9 for for IN 10_1101-2020_11_24_390039 109 10 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 109 11 to to IN 10_1101-2020_11_24_390039 109 12 mis- mis- NN 10_1101-2020_11_24_390039 109 13 .CC .CC : 10_1101-2020_11_24_390039 109 14 - - : 10_1101-2020_11_24_390039 109 15 BY by IN 10_1101-2020_11_24_390039 109 16 - - HYPH 10_1101-2020_11_24_390039 109 17 ND ND NNP 10_1101-2020_11_24_390039 109 18 4.0 4.0 CD 10_1101-2020_11_24_390039 109 19 International international JJ 10_1101-2020_11_24_390039 109 20 licensemade licensemade NN 10_1101-2020_11_24_390039 109 21 available available JJ 10_1101-2020_11_24_390039 109 22 under under IN 10_1101-2020_11_24_390039 109 23 a a DT 10_1101-2020_11_24_390039 109 24 ( ( -LRB- 10_1101-2020_11_24_390039 109 25 which which WDT 10_1101-2020_11_24_390039 109 26 was be VBD 10_1101-2020_11_24_390039 109 27 not not RB 10_1101-2020_11_24_390039 109 28 certified certify VBN 10_1101-2020_11_24_390039 109 29 by by IN 10_1101-2020_11_24_390039 109 30 peer peer NN 10_1101-2020_11_24_390039 109 31 review review NN 10_1101-2020_11_24_390039 109 32 ) ) -RRB- 10_1101-2020_11_24_390039 109 33 is be VBZ 10_1101-2020_11_24_390039 109 34 the the DT 10_1101-2020_11_24_390039 109 35 author author NN 10_1101-2020_11_24_390039 109 36 / / SYM 10_1101-2020_11_24_390039 109 37 funder funder NN 10_1101-2020_11_24_390039 109 38 , , , 10_1101-2020_11_24_390039 109 39 who who WP 10_1101-2020_11_24_390039 109 40 has have VBZ 10_1101-2020_11_24_390039 109 41 granted grant VBN 10_1101-2020_11_24_390039 109 42 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 109 43 a a DT 10_1101-2020_11_24_390039 109 44 license license NN 10_1101-2020_11_24_390039 109 45 to to TO 10_1101-2020_11_24_390039 109 46 display display VB 10_1101-2020_11_24_390039 109 47 the the DT 10_1101-2020_11_24_390039 109 48 preprint preprint NN 10_1101-2020_11_24_390039 109 49 in in IN 10_1101-2020_11_24_390039 109 50 perpetuity perpetuity NN 10_1101-2020_11_24_390039 109 51 . . . 10_1101-2020_11_24_390039 110 1 It -PRON- PRP 10_1101-2020_11_24_390039 110 2 is be VBZ 10_1101-2020_11_24_390039 110 3 The the DT 10_1101-2020_11_24_390039 110 4 copyright copyright NN 10_1101-2020_11_24_390039 110 5 holder holder NN 10_1101-2020_11_24_390039 110 6 for for IN 10_1101-2020_11_24_390039 110 7 this this DT 10_1101-2020_11_24_390039 110 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 110 9 version version NN 10_1101-2020_11_24_390039 110 10 posted post VBD 10_1101-2020_11_24_390039 110 11 January January NNP 10_1101-2020_11_24_390039 110 12 5 5 CD 10_1101-2020_11_24_390039 110 13 , , , 10_1101-2020_11_24_390039 110 14 2021 2021 CD 10_1101-2020_11_24_390039 110 15 . . . 10_1101-2020_11_24_390039 110 16 ; ; : 10_1101-2020_11_24_390039 110 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 110 18 : : : 10_1101-2020_11_24_390039 110 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 110 20 preprint preprint JJ 10_1101-2020_11_24_390039 110 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 110 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 110 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 110 24 - - HYPH 10_1101-2020_11_24_390039 110 25 F F NNP 10_1101-2020_11_24_390039 110 26 as as IN 10_1101-2020_11_24_390039 110 27 a a DT 10_1101-2020_11_24_390039 110 28 potential potential JJ 10_1101-2020_11_24_390039 110 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 110 30 agent agent NN 10_1101-2020_11_24_390039 110 31 Page Page NNP 10_1101-2020_11_24_390039 110 32 8 8 CD 10_1101-2020_11_24_390039 110 33 of of IN 10_1101-2020_11_24_390039 110 34 21 21 CD 10_1101-2020_11_24_390039 110 35 insert insert NN 10_1101-2020_11_24_390039 110 36 ( ( -LRB- 10_1101-2020_11_24_390039 110 37 cf cf NN 10_1101-2020_11_24_390039 110 38 . . . 10_1101-2020_11_24_390039 111 1 Chitwood Chitwood NNP 10_1101-2020_11_24_390039 111 2 et et NNP 10_1101-2020_11_24_390039 111 3 al al NNP 10_1101-2020_11_24_390039 111 4 . . NNP 10_1101-2020_11_24_390039 111 5 , , , 10_1101-2020_11_24_390039 111 6 2018 2018 CD 10_1101-2020_11_24_390039 111 7 ) ) -RRB- 10_1101-2020_11_24_390039 111 8 , , , 10_1101-2020_11_24_390039 111 9 perhaps perhaps RB 10_1101-2020_11_24_390039 111 10 as as IN 10_1101-2020_11_24_390039 111 11 a a DT 10_1101-2020_11_24_390039 111 12 consequence consequence NN 10_1101-2020_11_24_390039 111 13 of of IN 10_1101-2020_11_24_390039 111 14 disruption disruption NN 10_1101-2020_11_24_390039 111 15 to to IN 10_1101-2020_11_24_390039 111 16 the the DT 10_1101-2020_11_24_390039 111 17 translocation translocation NN 10_1101-2020_11_24_390039 111 18 of of IN 10_1101-2020_11_24_390039 111 19 its -PRON- PRP$ 10_1101-2020_11_24_390039 111 20 C c NN 10_1101-2020_11_24_390039 111 21 - - HYPH 10_1101-2020_11_24_390039 111 22 terminus terminus NN 10_1101-2020_11_24_390039 111 23 ( ( -LRB- 10_1101-2020_11_24_390039 111 24 Fig fig NN 10_1101-2020_11_24_390039 111 25 . . . 10_1101-2020_11_24_390039 112 1 S2E s2e LS 10_1101-2020_11_24_390039 112 2 ) ) -RRB- 10_1101-2020_11_24_390039 112 3 . . . 10_1101-2020_11_24_390039 113 1 Taken take VBN 10_1101-2020_11_24_390039 113 2 together together RB 10_1101-2020_11_24_390039 113 3 , , , 10_1101-2020_11_24_390039 113 4 our -PRON- PRP$ 10_1101-2020_11_24_390039 113 5 data datum NNS 10_1101-2020_11_24_390039 113 6 establish establish VBP 10_1101-2020_11_24_390039 113 7 that that IN 10_1101-2020_11_24_390039 113 8 , , , 10_1101-2020_11_24_390039 113 9 analogous analogous JJ 10_1101-2020_11_24_390039 113 10 to to IN 10_1101-2020_11_24_390039 113 11 human human JJ 10_1101-2020_11_24_390039 113 12 membrane membrane NN 10_1101-2020_11_24_390039 113 13 and and CC 10_1101-2020_11_24_390039 113 14 secretory secretory NN 10_1101-2020_11_24_390039 113 15 proteins protein NNS 10_1101-2020_11_24_390039 113 16 , , , 10_1101-2020_11_24_390039 113 17 the the DT 10_1101-2020_11_24_390039 113 18 principal principal JJ 10_1101-2020_11_24_390039 113 19 molecular molecular JJ 10_1101-2020_11_24_390039 113 20 basis basis NN 10_1101-2020_11_24_390039 113 21 for for IN 10_1101-2020_11_24_390039 113 22 the the DT 10_1101-2020_11_24_390039 113 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 113 24 - - HYPH 10_1101-2020_11_24_390039 113 25 F F NNP 10_1101-2020_11_24_390039 113 26 - - HYPH 10_1101-2020_11_24_390039 113 27 sensitivity sensitivity NN 10_1101-2020_11_24_390039 113 28 of of IN 10_1101-2020_11_24_390039 113 29 the the DT 10_1101-2020_11_24_390039 113 30 SARS SARS NNP 10_1101-2020_11_24_390039 113 31 - - HYPH 10_1101-2020_11_24_390039 113 32 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 113 33 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 113 34 and and CC 10_1101-2020_11_24_390039 113 35 S S NNP 10_1101-2020_11_24_390039 113 36 proteins protein NNS 10_1101-2020_11_24_390039 113 37 is be VBZ 10_1101-2020_11_24_390039 113 38 their -PRON- PRP$ 10_1101-2020_11_24_390039 113 39 dependence dependence NN 10_1101-2020_11_24_390039 113 40 on on IN 10_1101-2020_11_24_390039 113 41 Sec61-mediated sec61-mediated JJ 10_1101-2020_11_24_390039 113 42 protein protein NN 10_1101-2020_11_24_390039 113 43 translocation translocation NN 10_1101-2020_11_24_390039 113 44 into into IN 10_1101-2020_11_24_390039 113 45 and and CC 10_1101-2020_11_24_390039 113 46 across across IN 10_1101-2020_11_24_390039 113 47 the the DT 10_1101-2020_11_24_390039 113 48 ER ER NNP 10_1101-2020_11_24_390039 113 49 membrane membrane NN 10_1101-2020_11_24_390039 113 50 . . . 10_1101-2020_11_24_390039 114 1 In in IN 10_1101-2020_11_24_390039 114 2 contrast contrast NN 10_1101-2020_11_24_390039 114 3 , , , 10_1101-2020_11_24_390039 114 4 the the DT 10_1101-2020_11_24_390039 114 5 E e NN 10_1101-2020_11_24_390039 114 6 , , , 10_1101-2020_11_24_390039 114 7 M M NNP 10_1101-2020_11_24_390039 114 8 , , , 10_1101-2020_11_24_390039 114 9 and and CC 10_1101-2020_11_24_390039 114 10 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 114 11 proteins protein NNS 10_1101-2020_11_24_390039 114 12 appear appear VBP 10_1101-2020_11_24_390039 114 13 capable capable JJ 10_1101-2020_11_24_390039 114 14 of of IN 10_1101-2020_11_24_390039 114 15 exploiting exploit VBG 10_1101-2020_11_24_390039 114 16 one one CD 10_1101-2020_11_24_390039 114 17 or or CC 10_1101-2020_11_24_390039 114 18 more more JJR 10_1101-2020_11_24_390039 114 19 alternative alternative JJ 10_1101-2020_11_24_390039 114 20 membrane membrane NN 10_1101-2020_11_24_390039 114 21 insertion insertion NN 10_1101-2020_11_24_390039 114 22 / / SYM 10_1101-2020_11_24_390039 114 23 translocation translocation NN 10_1101-2020_11_24_390039 114 24 pathways pathway NNS 10_1101-2020_11_24_390039 114 25 that that WDT 10_1101-2020_11_24_390039 114 26 can can MD 10_1101-2020_11_24_390039 114 27 bypass bypass VB 10_1101-2020_11_24_390039 114 28 the the DT 10_1101-2020_11_24_390039 114 29 translocase translocase NN 10_1101-2020_11_24_390039 114 30 activity activity NN 10_1101-2020_11_24_390039 114 31 of of IN 10_1101-2020_11_24_390039 114 32 the the DT 10_1101-2020_11_24_390039 114 33 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 114 34 complex complex NN 10_1101-2020_11_24_390039 114 35 . . . 10_1101-2020_11_24_390039 115 1 These these DT 10_1101-2020_11_24_390039 115 2 alternatives alternative NNS 10_1101-2020_11_24_390039 115 3 most most RBS 10_1101-2020_11_24_390039 115 4 likely likely RB 10_1101-2020_11_24_390039 115 5 include include VBP 10_1101-2020_11_24_390039 115 6 a a DT 10_1101-2020_11_24_390039 115 7 recently recently RB 10_1101-2020_11_24_390039 115 8 described describe VBN 10_1101-2020_11_24_390039 115 9 route route NN 10_1101-2020_11_24_390039 115 10 for for IN 10_1101-2020_11_24_390039 115 11 type type NN 10_1101-2020_11_24_390039 115 12 III III NNP 10_1101-2020_11_24_390039 115 13 TMP TMP NNP 10_1101-2020_11_24_390039 115 14 insertion insertion NN 10_1101-2020_11_24_390039 115 15 that that WDT 10_1101-2020_11_24_390039 115 16 requires require VBZ 10_1101-2020_11_24_390039 115 17 the the DT 10_1101-2020_11_24_390039 115 18 insertase insertase NN 10_1101-2020_11_24_390039 115 19 function function NN 10_1101-2020_11_24_390039 115 20 of of IN 10_1101-2020_11_24_390039 115 21 the the DT 10_1101-2020_11_24_390039 115 22 EMC EMC NNP 10_1101-2020_11_24_390039 115 23 ( ( -LRB- 10_1101-2020_11_24_390039 115 24 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 115 25 et et NNP 10_1101-2020_11_24_390039 115 26 al al NNP 10_1101-2020_11_24_390039 115 27 . . NNP 10_1101-2020_11_24_390039 115 28 , , , 10_1101-2020_11_24_390039 115 29 2020 2020 CD 10_1101-2020_11_24_390039 115 30 submitted submit VBN 10_1101-2020_11_24_390039 115 31 ) ) -RRB- 10_1101-2020_11_24_390039 115 32 , , , 10_1101-2020_11_24_390039 115 33 which which WDT 10_1101-2020_11_24_390039 115 34 our -PRON- PRP$ 10_1101-2020_11_24_390039 115 35 data datum NNS 10_1101-2020_11_24_390039 115 36 suggest suggest VBP 10_1101-2020_11_24_390039 115 37 is be VBZ 10_1101-2020_11_24_390039 115 38 also also RB 10_1101-2020_11_24_390039 115 39 sufficient sufficient JJ 10_1101-2020_11_24_390039 115 40 to to TO 10_1101-2020_11_24_390039 115 41 confer confer VB 10_1101-2020_11_24_390039 115 42 Ipom Ipom NNP 10_1101-2020_11_24_390039 115 43 - - HYPH 10_1101-2020_11_24_390039 115 44 F F NNP 10_1101-2020_11_24_390039 115 45 - - HYPH 10_1101-2020_11_24_390039 115 46 resistance resistance NN 10_1101-2020_11_24_390039 115 47 to to IN 10_1101-2020_11_24_390039 115 48 the the DT 10_1101-2020_11_24_390039 115 49 viral viral JJ 10_1101-2020_11_24_390039 115 50 E e NN 10_1101-2020_11_24_390039 115 51 protein protein NN 10_1101-2020_11_24_390039 115 52 and and CC 10_1101-2020_11_24_390039 115 53 at at IN 10_1101-2020_11_24_390039 115 54 least least JJS 10_1101-2020_11_24_390039 115 55 the the DT 10_1101-2020_11_24_390039 115 56 first first JJ 10_1101-2020_11_24_390039 115 57 TM TM NNP 10_1101-2020_11_24_390039 115 58 - - HYPH 10_1101-2020_11_24_390039 115 59 span span NN 10_1101-2020_11_24_390039 115 60 of of IN 10_1101-2020_11_24_390039 115 61 the the DT 10_1101-2020_11_24_390039 115 62 viral viral JJ 10_1101-2020_11_24_390039 115 63 M M NNP 10_1101-2020_11_24_390039 115 64 protein protein NN 10_1101-2020_11_24_390039 115 65 . . . 10_1101-2020_11_24_390039 116 1 Concluding conclude VBG 10_1101-2020_11_24_390039 116 2 Remarks Remarks NNPS 10_1101-2020_11_24_390039 116 3 We -PRON- PRP 10_1101-2020_11_24_390039 116 4 conclude conclude VBP 10_1101-2020_11_24_390039 116 5 , , , 10_1101-2020_11_24_390039 116 6 that that DT 10_1101-2020_11_24_390039 116 7 Sec61-selective sec61-selective JJ 10_1101-2020_11_24_390039 116 8 protein protein NN 10_1101-2020_11_24_390039 116 9 translocation translocation NN 10_1101-2020_11_24_390039 116 10 inhibitors inhibitor NNS 10_1101-2020_11_24_390039 116 11 like like IN 10_1101-2020_11_24_390039 116 12 Ipom Ipom NNP 10_1101-2020_11_24_390039 116 13 - - HYPH 10_1101-2020_11_24_390039 116 14 F F NNP 10_1101-2020_11_24_390039 116 15 hold hold NN 10_1101-2020_11_24_390039 116 16 promise promise NN 10_1101-2020_11_24_390039 116 17 as as IN 10_1101-2020_11_24_390039 116 18 broad broad JJ 10_1101-2020_11_24_390039 116 19 - - HYPH 10_1101-2020_11_24_390039 116 20 spectrum spectrum NN 10_1101-2020_11_24_390039 116 21 antivirals antiviral NNS 10_1101-2020_11_24_390039 116 22 that that WDT 10_1101-2020_11_24_390039 116 23 may may MD 10_1101-2020_11_24_390039 116 24 exert exert VB 10_1101-2020_11_24_390039 116 25 a a DT 10_1101-2020_11_24_390039 116 26 therapeutic therapeutic JJ 10_1101-2020_11_24_390039 116 27 effect effect NN 10_1101-2020_11_24_390039 116 28 by by IN 10_1101-2020_11_24_390039 116 29 selectively selectively RB 10_1101-2020_11_24_390039 116 30 inhibiting inhibit VBG 10_1101-2020_11_24_390039 116 31 the the DT 10_1101-2020_11_24_390039 116 32 ER ER NNP 10_1101-2020_11_24_390039 116 33 translocation translocation NN 10_1101-2020_11_24_390039 116 34 of of IN 10_1101-2020_11_24_390039 116 35 viral viral JJ 10_1101-2020_11_24_390039 116 36 and/or and/or CC 10_1101-2020_11_24_390039 116 37 host host NN 10_1101-2020_11_24_390039 116 38 proteins protein NNS 10_1101-2020_11_24_390039 116 39 which which WDT 10_1101-2020_11_24_390039 116 40 are be VBP 10_1101-2020_11_24_390039 116 41 crucial crucial JJ 10_1101-2020_11_24_390039 116 42 to to IN 10_1101-2020_11_24_390039 116 43 viral viral JJ 10_1101-2020_11_24_390039 116 44 infection infection NN 10_1101-2020_11_24_390039 116 45 and and CC 10_1101-2020_11_24_390039 116 46 propagation propagation NN 10_1101-2020_11_24_390039 116 47 ( ( -LRB- 10_1101-2020_11_24_390039 116 48 Mast Mast NNP 10_1101-2020_11_24_390039 116 49 et et NNP 10_1101-2020_11_24_390039 116 50 al al NNP 10_1101-2020_11_24_390039 116 51 . . NNP 10_1101-2020_11_24_390039 116 52 , , , 10_1101-2020_11_24_390039 116 53 2020 2020 CD 10_1101-2020_11_24_390039 116 54 ) ) -RRB- 10_1101-2020_11_24_390039 116 55 . . . 10_1101-2020_11_24_390039 117 1 In in IN 10_1101-2020_11_24_390039 117 2 the the DT 10_1101-2020_11_24_390039 117 3 context context NN 10_1101-2020_11_24_390039 117 4 of of IN 10_1101-2020_11_24_390039 117 5 SARS SARS NNP 10_1101-2020_11_24_390039 117 6 - - HYPH 10_1101-2020_11_24_390039 117 7 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 117 8 , , , 10_1101-2020_11_24_390039 117 9 integration integration NN 10_1101-2020_11_24_390039 117 10 of of IN 10_1101-2020_11_24_390039 117 11 the the DT 10_1101-2020_11_24_390039 117 12 viral viral JJ 10_1101-2020_11_24_390039 117 13 S S NNP 10_1101-2020_11_24_390039 117 14 protein protein NN 10_1101-2020_11_24_390039 117 15 and and CC 10_1101-2020_11_24_390039 117 16 its -PRON- PRP$ 10_1101-2020_11_24_390039 117 17 host host NN 10_1101-2020_11_24_390039 117 18 cell cell NN 10_1101-2020_11_24_390039 117 19 receptor receptor NN 10_1101-2020_11_24_390039 117 20 , , , 10_1101-2020_11_24_390039 117 21 ACE2 ACE2 NNP 10_1101-2020_11_24_390039 117 22 , , , 10_1101-2020_11_24_390039 117 23 into into IN 10_1101-2020_11_24_390039 117 24 the the DT 10_1101-2020_11_24_390039 117 25 ER ER NNP 10_1101-2020_11_24_390039 117 26 membrane membrane NN 10_1101-2020_11_24_390039 117 27 is be VBZ 10_1101-2020_11_24_390039 117 28 significantly significantly RB 10_1101-2020_11_24_390039 117 29 reduced reduce VBN 10_1101-2020_11_24_390039 117 30 by by IN 10_1101-2020_11_24_390039 117 31 Ipom Ipom NNP 10_1101-2020_11_24_390039 117 32 - - HYPH 10_1101-2020_11_24_390039 117 33 F F NNP 10_1101-2020_11_24_390039 117 34 ( ( -LRB- 10_1101-2020_11_24_390039 117 35 Fig Fig NNP 10_1101-2020_11_24_390039 117 36 . . . 10_1101-2020_11_24_390039 118 1 2C 2C NNP 10_1101-2020_11_24_390039 118 2 , , , 10_1101-2020_11_24_390039 118 3 3B 3B NNP 10_1101-2020_11_24_390039 118 4 ) ) -RRB- 10_1101-2020_11_24_390039 118 5 . . . 10_1101-2020_11_24_390039 119 1 Likewise likewise RB 10_1101-2020_11_24_390039 119 2 , , , 10_1101-2020_11_24_390039 119 3 translocation translocation NN 10_1101-2020_11_24_390039 119 4 of of IN 10_1101-2020_11_24_390039 119 5 the the DT 10_1101-2020_11_24_390039 119 6 viral viral JJ 10_1101-2020_11_24_390039 119 7 ORF8 orf8 JJ 10_1101-2020_11_24_390039 119 8 protein protein NN 10_1101-2020_11_24_390039 119 9 across across IN 10_1101-2020_11_24_390039 119 10 the the DT 10_1101-2020_11_24_390039 119 11 ER ER NNP 10_1101-2020_11_24_390039 119 12 membrane membrane NN 10_1101-2020_11_24_390039 119 13 and and CC 10_1101-2020_11_24_390039 119 14 into into IN 10_1101-2020_11_24_390039 119 15 its -PRON- PRP$ 10_1101-2020_11_24_390039 119 16 lumen luman NNS 10_1101-2020_11_24_390039 119 17 is be VBZ 10_1101-2020_11_24_390039 119 18 substantially substantially RB 10_1101-2020_11_24_390039 119 19 diminished diminish VBN 10_1101-2020_11_24_390039 119 20 ( ( -LRB- 10_1101-2020_11_24_390039 119 21 Fig fig NN 10_1101-2020_11_24_390039 119 22 . . . 10_1101-2020_11_24_390039 120 1 2C 2C NNP 10_1101-2020_11_24_390039 120 2 , , , 10_1101-2020_11_24_390039 120 3 3C 3c NN 10_1101-2020_11_24_390039 120 4 ) ) -RRB- 10_1101-2020_11_24_390039 120 5 . . . 10_1101-2020_11_24_390039 121 1 The the DT 10_1101-2020_11_24_390039 121 2 binding binding NN 10_1101-2020_11_24_390039 121 3 of of IN 10_1101-2020_11_24_390039 121 4 the the DT 10_1101-2020_11_24_390039 121 5 viral viral JJ 10_1101-2020_11_24_390039 121 6 S S NNP 10_1101-2020_11_24_390039 121 7 protein protein NN 10_1101-2020_11_24_390039 121 8 to to IN 10_1101-2020_11_24_390039 121 9 cell cell NN 10_1101-2020_11_24_390039 121 10 surface surface NN 10_1101-2020_11_24_390039 121 11 ACE2 ACE2 NNP 10_1101-2020_11_24_390039 121 12 is be VBZ 10_1101-2020_11_24_390039 121 13 a a DT 10_1101-2020_11_24_390039 121 14 key key JJ 10_1101-2020_11_24_390039 121 15 step step NN 10_1101-2020_11_24_390039 121 16 in in IN 10_1101-2020_11_24_390039 121 17 host host NN 10_1101-2020_11_24_390039 121 18 cell cell NN 10_1101-2020_11_24_390039 121 19 infection infection NN 10_1101-2020_11_24_390039 121 20 ( ( -LRB- 10_1101-2020_11_24_390039 121 21 Drew Drew NNP 10_1101-2020_11_24_390039 121 22 and and CC 10_1101-2020_11_24_390039 121 23 Janes Janes NNP 10_1101-2020_11_24_390039 121 24 , , , 10_1101-2020_11_24_390039 121 25 2020 2020 CD 10_1101-2020_11_24_390039 121 26 ) ) -RRB- 10_1101-2020_11_24_390039 121 27 , , , 10_1101-2020_11_24_390039 121 28 whilst whilst IN 10_1101-2020_11_24_390039 121 29 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 121 30 may may MD 10_1101-2020_11_24_390039 121 31 protect protect VB 10_1101-2020_11_24_390039 121 32 SARS SARS NNP 10_1101-2020_11_24_390039 121 33 - - HYPH 10_1101-2020_11_24_390039 121 34 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 121 35 infected infected JJ 10_1101-2020_11_24_390039 121 36 cells cell NNS 10_1101-2020_11_24_390039 121 37 against against IN 10_1101-2020_11_24_390039 121 38 host host NN 10_1101-2020_11_24_390039 121 39 cytotoxic cytotoxic NNP 10_1101-2020_11_24_390039 121 40 T T NNP 10_1101-2020_11_24_390039 121 41 lymphocytes lymphocyte NNS 10_1101-2020_11_24_390039 121 42 ( ( -LRB- 10_1101-2020_11_24_390039 121 43 Zhang Zhang NNP 10_1101-2020_11_24_390039 121 44 et et NNP 10_1101-2020_11_24_390039 121 45 al al NNP 10_1101-2020_11_24_390039 121 46 . . NNP 10_1101-2020_11_24_390039 121 47 , , , 10_1101-2020_11_24_390039 121 48 2020 2020 CD 10_1101-2020_11_24_390039 121 49 ) ) -RRB- 10_1101-2020_11_24_390039 121 50 , , , 10_1101-2020_11_24_390039 121 51 making make VBG 10_1101-2020_11_24_390039 121 52 all all DT 10_1101-2020_11_24_390039 121 53 three three CD 10_1101-2020_11_24_390039 121 54 of of IN 10_1101-2020_11_24_390039 121 55 these these DT 10_1101-2020_11_24_390039 121 56 proteins protein NNS 10_1101-2020_11_24_390039 121 57 viable viable JJ 10_1101-2020_11_24_390039 121 58 therapeutic therapeutic JJ 10_1101-2020_11_24_390039 121 59 targets target NNS 10_1101-2020_11_24_390039 121 60 ( ( -LRB- 10_1101-2020_11_24_390039 121 61 Drew Drew NNP 10_1101-2020_11_24_390039 121 62 and and CC 10_1101-2020_11_24_390039 121 63 Janes Janes NNP 10_1101-2020_11_24_390039 121 64 , , , 10_1101-2020_11_24_390039 121 65 2020 2020 CD 10_1101-2020_11_24_390039 121 66 ; ; : 10_1101-2020_11_24_390039 121 67 Li Li NNP 10_1101-2020_11_24_390039 121 68 et et NNP 10_1101-2020_11_24_390039 121 69 al al NNP 10_1101-2020_11_24_390039 121 70 . . NNP 10_1101-2020_11_24_390039 121 71 , , , 10_1101-2020_11_24_390039 121 72 2020 2020 CD 10_1101-2020_11_24_390039 121 73 ; ; : 10_1101-2020_11_24_390039 121 74 Young Young NNP 10_1101-2020_11_24_390039 121 75 et et NNP 10_1101-2020_11_24_390039 121 76 al al NNP 10_1101-2020_11_24_390039 121 77 . . NNP 10_1101-2020_11_24_390039 121 78 , , , 10_1101-2020_11_24_390039 121 79 2020 2020 CD 10_1101-2020_11_24_390039 121 80 ) ) -RRB- 10_1101-2020_11_24_390039 121 81 . . . 10_1101-2020_11_24_390039 122 1 Like like IN 10_1101-2020_11_24_390039 122 2 other other JJ 10_1101-2020_11_24_390039 122 3 small small JJ 10_1101-2020_11_24_390039 122 4 molecule molecule NN 10_1101-2020_11_24_390039 122 5 inhibitors inhibitor NNS 10_1101-2020_11_24_390039 122 6 that that WDT 10_1101-2020_11_24_390039 122 7 target target VBP 10_1101-2020_11_24_390039 122 8 fundamental fundamental JJ 10_1101-2020_11_24_390039 122 9 cellular cellular JJ 10_1101-2020_11_24_390039 122 10 pathways pathway NNS 10_1101-2020_11_24_390039 122 11 ( ( -LRB- 10_1101-2020_11_24_390039 122 12 Bojkova Bojkova NNP 10_1101-2020_11_24_390039 122 13 et et NNP 10_1101-2020_11_24_390039 122 14 al al NNP 10_1101-2020_11_24_390039 122 15 . . . 10_1101-2020_11_24_390039 123 1 2020 2020 CD 10_1101-2020_11_24_390039 123 2 ) ) -RRB- 10_1101-2020_11_24_390039 123 3 , , , 10_1101-2020_11_24_390039 123 4 the the DT 10_1101-2020_11_24_390039 123 5 broad broad RB 10_1101-2020_11_24_390039 123 6 - - HYPH 10_1101-2020_11_24_390039 123 7 ranging range VBG 10_1101-2020_11_24_390039 123 8 effects effect NNS 10_1101-2020_11_24_390039 123 9 of of IN 10_1101-2020_11_24_390039 123 10 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 123 11 inhibitors inhibitor NNS 10_1101-2020_11_24_390039 123 12 on on IN 10_1101-2020_11_24_390039 123 13 host host NN 10_1101-2020_11_24_390039 123 14 cell cell NN 10_1101-2020_11_24_390039 123 15 membrane membrane NN 10_1101-2020_11_24_390039 123 16 and and CC 10_1101-2020_11_24_390039 123 17 secretory secretory JJ 10_1101-2020_11_24_390039 123 18 protein protein NN 10_1101-2020_11_24_390039 123 19 synthesis synthesis NN 10_1101-2020_11_24_390039 123 20 ( ( -LRB- 10_1101-2020_11_24_390039 123 21 Morel Morel NNP 10_1101-2020_11_24_390039 123 22 et et NNP 10_1101-2020_11_24_390039 123 23 al al NNP 10_1101-2020_11_24_390039 123 24 . . NNP 10_1101-2020_11_24_390039 123 25 , , , 10_1101-2020_11_24_390039 123 26 2018 2018 CD 10_1101-2020_11_24_390039 123 27 ; ; : 10_1101-2020_11_24_390039 123 28 Zong Zong NNP 10_1101-2020_11_24_390039 123 29 et et NNP 10_1101-2020_11_24_390039 123 30 al al NNP 10_1101-2020_11_24_390039 123 31 . . . 10_1101-2020_11_24_390039 124 1 2019 2019 CD 10_1101-2020_11_24_390039 124 2 ) ) -RRB- 10_1101-2020_11_24_390039 124 3 , , , 10_1101-2020_11_24_390039 124 4 including include VBG 10_1101-2020_11_24_390039 124 5 the the DT 10_1101-2020_11_24_390039 124 6 strong strong JJ 10_1101-2020_11_24_390039 124 7 in in IN 10_1101-2020_11_24_390039 124 8 vitro vitro FW 10_1101-2020_11_24_390039 124 9 effect effect NN 10_1101-2020_11_24_390039 124 10 of of IN 10_1101-2020_11_24_390039 124 11 Ipom Ipom NNP 10_1101-2020_11_24_390039 124 12 - - HYPH 10_1101-2020_11_24_390039 124 13 F F NNP 10_1101-2020_11_24_390039 124 14 on on IN 10_1101-2020_11_24_390039 124 15 ACE2 ACE2 NNP 10_1101-2020_11_24_390039 124 16 biogenesis biogenesis NN 10_1101-2020_11_24_390039 124 17 ( ( -LRB- 10_1101-2020_11_24_390039 124 18 cf cf NN 10_1101-2020_11_24_390039 124 19 . . . 10_1101-2020_11_24_390039 125 1 Grob Grob NNP 10_1101-2020_11_24_390039 125 2 et et NNP 10_1101-2020_11_24_390039 125 3 al al NNP 10_1101-2020_11_24_390039 125 4 . . . 10_1101-2020_11_24_390039 126 1 .CC .CC NFP 10_1101-2020_11_24_390039 126 2 - - : 10_1101-2020_11_24_390039 126 3 BY by IN 10_1101-2020_11_24_390039 126 4 - - HYPH 10_1101-2020_11_24_390039 126 5 ND ND NNP 10_1101-2020_11_24_390039 126 6 4.0 4.0 CD 10_1101-2020_11_24_390039 126 7 International international JJ 10_1101-2020_11_24_390039 126 8 licensemade licensemade NN 10_1101-2020_11_24_390039 126 9 available available JJ 10_1101-2020_11_24_390039 126 10 under under IN 10_1101-2020_11_24_390039 126 11 a a DT 10_1101-2020_11_24_390039 126 12 ( ( -LRB- 10_1101-2020_11_24_390039 126 13 which which WDT 10_1101-2020_11_24_390039 126 14 was be VBD 10_1101-2020_11_24_390039 126 15 not not RB 10_1101-2020_11_24_390039 126 16 certified certify VBN 10_1101-2020_11_24_390039 126 17 by by IN 10_1101-2020_11_24_390039 126 18 peer peer NN 10_1101-2020_11_24_390039 126 19 review review NN 10_1101-2020_11_24_390039 126 20 ) ) -RRB- 10_1101-2020_11_24_390039 126 21 is be VBZ 10_1101-2020_11_24_390039 126 22 the the DT 10_1101-2020_11_24_390039 126 23 author author NN 10_1101-2020_11_24_390039 126 24 / / SYM 10_1101-2020_11_24_390039 126 25 funder funder NN 10_1101-2020_11_24_390039 126 26 , , , 10_1101-2020_11_24_390039 126 27 who who WP 10_1101-2020_11_24_390039 126 28 has have VBZ 10_1101-2020_11_24_390039 126 29 granted grant VBN 10_1101-2020_11_24_390039 126 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 126 31 a a DT 10_1101-2020_11_24_390039 126 32 license license NN 10_1101-2020_11_24_390039 126 33 to to TO 10_1101-2020_11_24_390039 126 34 display display VB 10_1101-2020_11_24_390039 126 35 the the DT 10_1101-2020_11_24_390039 126 36 preprint preprint NN 10_1101-2020_11_24_390039 126 37 in in IN 10_1101-2020_11_24_390039 126 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 126 39 . . . 10_1101-2020_11_24_390039 127 1 It -PRON- PRP 10_1101-2020_11_24_390039 127 2 is be VBZ 10_1101-2020_11_24_390039 127 3 The the DT 10_1101-2020_11_24_390039 127 4 copyright copyright NN 10_1101-2020_11_24_390039 127 5 holder holder NN 10_1101-2020_11_24_390039 127 6 for for IN 10_1101-2020_11_24_390039 127 7 this this DT 10_1101-2020_11_24_390039 127 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 127 9 version version NN 10_1101-2020_11_24_390039 127 10 posted post VBD 10_1101-2020_11_24_390039 127 11 January January NNP 10_1101-2020_11_24_390039 127 12 5 5 CD 10_1101-2020_11_24_390039 127 13 , , , 10_1101-2020_11_24_390039 127 14 2021 2021 CD 10_1101-2020_11_24_390039 127 15 . . . 10_1101-2020_11_24_390039 127 16 ; ; : 10_1101-2020_11_24_390039 127 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 127 18 : : : 10_1101-2020_11_24_390039 127 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 127 20 preprint preprint JJ 10_1101-2020_11_24_390039 127 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 127 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 127 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 127 24 - - HYPH 10_1101-2020_11_24_390039 127 25 F F NNP 10_1101-2020_11_24_390039 127 26 as as IN 10_1101-2020_11_24_390039 127 27 a a DT 10_1101-2020_11_24_390039 127 28 potential potential JJ 10_1101-2020_11_24_390039 127 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 127 30 agent agent NN 10_1101-2020_11_24_390039 127 31 Page Page NNP 10_1101-2020_11_24_390039 127 32 9 9 CD 10_1101-2020_11_24_390039 127 33 of of IN 10_1101-2020_11_24_390039 127 34 21 21 CD 10_1101-2020_11_24_390039 127 35 2020 2020 CD 10_1101-2020_11_24_390039 127 36 ) ) -RRB- 10_1101-2020_11_24_390039 127 37 , , , 10_1101-2020_11_24_390039 127 38 present present VB 10_1101-2020_11_24_390039 127 39 an an DT 10_1101-2020_11_24_390039 127 40 obvious obvious JJ 10_1101-2020_11_24_390039 127 41 hurdle hurdle NN 10_1101-2020_11_24_390039 127 42 to to IN 10_1101-2020_11_24_390039 127 43 their -PRON- PRP$ 10_1101-2020_11_24_390039 127 44 future future JJ 10_1101-2020_11_24_390039 127 45 use use NN 10_1101-2020_11_24_390039 127 46 . . . 10_1101-2020_11_24_390039 128 1 Nevertheless nevertheless RB 10_1101-2020_11_24_390039 128 2 , , , 10_1101-2020_11_24_390039 128 3 given give VBN 10_1101-2020_11_24_390039 128 4 that that IN 10_1101-2020_11_24_390039 128 5 Ipom Ipom NNP 10_1101-2020_11_24_390039 128 6 - - HYPH 10_1101-2020_11_24_390039 128 7 F F NNP 10_1101-2020_11_24_390039 128 8 is be VBZ 10_1101-2020_11_24_390039 128 9 a a DT 10_1101-2020_11_24_390039 128 10 potent potent JJ 10_1101-2020_11_24_390039 128 11 inhibitor inhibitor NN 10_1101-2020_11_24_390039 128 12 of of IN 10_1101-2020_11_24_390039 128 13 Sec61-mediated sec61-mediate VBN 10_1101-2020_11_24_390039 128 14 protein protein NN 10_1101-2020_11_24_390039 128 15 translocation translocation NN 10_1101-2020_11_24_390039 128 16 in in IN 10_1101-2020_11_24_390039 128 17 cell cell NN 10_1101-2020_11_24_390039 128 18 culture culture NN 10_1101-2020_11_24_390039 128 19 models model NNS 10_1101-2020_11_24_390039 128 20 ( ( -LRB- 10_1101-2020_11_24_390039 128 21 Zong Zong NNP 10_1101-2020_11_24_390039 128 22 et et NNP 10_1101-2020_11_24_390039 128 23 al al NNP 10_1101-2020_11_24_390039 128 24 . . NNP 10_1101-2020_11_24_390039 128 25 , , , 10_1101-2020_11_24_390039 128 26 2019 2019 CD 10_1101-2020_11_24_390039 128 27 ) ) -RRB- 10_1101-2020_11_24_390039 128 28 , , , 10_1101-2020_11_24_390039 128 29 and and CC 10_1101-2020_11_24_390039 128 30 appears appear VBZ 10_1101-2020_11_24_390039 128 31 well well RB 10_1101-2020_11_24_390039 128 32 tolerated tolerate VBN 10_1101-2020_11_24_390039 128 33 in in IN 10_1101-2020_11_24_390039 128 34 mice mouse NNS 10_1101-2020_11_24_390039 128 35 ( ( -LRB- 10_1101-2020_11_24_390039 128 36 Zong Zong NNP 10_1101-2020_11_24_390039 128 37 et et NNP 10_1101-2020_11_24_390039 128 38 al al NNP 10_1101-2020_11_24_390039 128 39 . . NNP 10_1101-2020_11_24_390039 128 40 , , , 10_1101-2020_11_24_390039 128 41 2020 2020 CD 10_1101-2020_11_24_390039 128 42 ) ) -RRB- 10_1101-2020_11_24_390039 128 43 , , , 10_1101-2020_11_24_390039 128 44 we -PRON- PRP 10_1101-2020_11_24_390039 128 45 propose propose VBP 10_1101-2020_11_24_390039 128 46 that that IN 10_1101-2020_11_24_390039 128 47 future future JJ 10_1101-2020_11_24_390039 128 48 studies study NNS 10_1101-2020_11_24_390039 128 49 investigating investigate VBG 10_1101-2020_11_24_390039 128 50 its -PRON- PRP$ 10_1101-2020_11_24_390039 128 51 effect effect NN 10_1101-2020_11_24_390039 128 52 on on IN 10_1101-2020_11_24_390039 128 53 SARS SARS NNP 10_1101-2020_11_24_390039 128 54 - - HYPH 10_1101-2020_11_24_390039 128 55 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 128 56 infection infection NN 10_1101-2020_11_24_390039 128 57 and and CC 10_1101-2020_11_24_390039 128 58 propagation propagation NN 10_1101-2020_11_24_390039 128 59 in in IN 10_1101-2020_11_24_390039 128 60 cellular cellular JJ 10_1101-2020_11_24_390039 128 61 models model NNS 10_1101-2020_11_24_390039 128 62 are be VBP 10_1101-2020_11_24_390039 128 63 clearly clearly RB 10_1101-2020_11_24_390039 128 64 warranted warrant VBN 10_1101-2020_11_24_390039 128 65 ( ( -LRB- 10_1101-2020_11_24_390039 128 66 cf cf NN 10_1101-2020_11_24_390039 128 67 . . . 10_1101-2020_11_24_390039 129 1 Bojkova Bojkova NNP 10_1101-2020_11_24_390039 129 2 et et NNP 10_1101-2020_11_24_390039 129 3 al al NNP 10_1101-2020_11_24_390039 129 4 . . . 10_1101-2020_11_24_390039 130 1 2020 2020 CD 10_1101-2020_11_24_390039 130 2 ) ) -RRB- 10_1101-2020_11_24_390039 130 3 . . . 10_1101-2020_11_24_390039 131 1 .CC .CC NFP 10_1101-2020_11_24_390039 131 2 - - : 10_1101-2020_11_24_390039 131 3 BY by IN 10_1101-2020_11_24_390039 131 4 - - HYPH 10_1101-2020_11_24_390039 131 5 ND ND NNP 10_1101-2020_11_24_390039 131 6 4.0 4.0 CD 10_1101-2020_11_24_390039 131 7 International international JJ 10_1101-2020_11_24_390039 131 8 licensemade licensemade NN 10_1101-2020_11_24_390039 131 9 available available JJ 10_1101-2020_11_24_390039 131 10 under under IN 10_1101-2020_11_24_390039 131 11 a a DT 10_1101-2020_11_24_390039 131 12 ( ( -LRB- 10_1101-2020_11_24_390039 131 13 which which WDT 10_1101-2020_11_24_390039 131 14 was be VBD 10_1101-2020_11_24_390039 131 15 not not RB 10_1101-2020_11_24_390039 131 16 certified certify VBN 10_1101-2020_11_24_390039 131 17 by by IN 10_1101-2020_11_24_390039 131 18 peer peer NN 10_1101-2020_11_24_390039 131 19 review review NN 10_1101-2020_11_24_390039 131 20 ) ) -RRB- 10_1101-2020_11_24_390039 131 21 is be VBZ 10_1101-2020_11_24_390039 131 22 the the DT 10_1101-2020_11_24_390039 131 23 author author NN 10_1101-2020_11_24_390039 131 24 / / SYM 10_1101-2020_11_24_390039 131 25 funder funder NN 10_1101-2020_11_24_390039 131 26 , , , 10_1101-2020_11_24_390039 131 27 who who WP 10_1101-2020_11_24_390039 131 28 has have VBZ 10_1101-2020_11_24_390039 131 29 granted grant VBN 10_1101-2020_11_24_390039 131 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 131 31 a a DT 10_1101-2020_11_24_390039 131 32 license license NN 10_1101-2020_11_24_390039 131 33 to to TO 10_1101-2020_11_24_390039 131 34 display display VB 10_1101-2020_11_24_390039 131 35 the the DT 10_1101-2020_11_24_390039 131 36 preprint preprint NN 10_1101-2020_11_24_390039 131 37 in in IN 10_1101-2020_11_24_390039 131 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 131 39 . . . 10_1101-2020_11_24_390039 132 1 It -PRON- PRP 10_1101-2020_11_24_390039 132 2 is be VBZ 10_1101-2020_11_24_390039 132 3 The the DT 10_1101-2020_11_24_390039 132 4 copyright copyright NN 10_1101-2020_11_24_390039 132 5 holder holder NN 10_1101-2020_11_24_390039 132 6 for for IN 10_1101-2020_11_24_390039 132 7 this this DT 10_1101-2020_11_24_390039 132 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 132 9 version version NN 10_1101-2020_11_24_390039 132 10 posted post VBD 10_1101-2020_11_24_390039 132 11 January January NNP 10_1101-2020_11_24_390039 132 12 5 5 CD 10_1101-2020_11_24_390039 132 13 , , , 10_1101-2020_11_24_390039 132 14 2021 2021 CD 10_1101-2020_11_24_390039 132 15 . . . 10_1101-2020_11_24_390039 132 16 ; ; : 10_1101-2020_11_24_390039 132 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 132 18 : : : 10_1101-2020_11_24_390039 132 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 132 20 preprint preprint JJ 10_1101-2020_11_24_390039 132 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 132 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 132 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 132 24 - - HYPH 10_1101-2020_11_24_390039 132 25 F F NNP 10_1101-2020_11_24_390039 132 26 as as IN 10_1101-2020_11_24_390039 132 27 a a DT 10_1101-2020_11_24_390039 132 28 potential potential JJ 10_1101-2020_11_24_390039 132 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 132 30 agent agent NN 10_1101-2020_11_24_390039 132 31 Page Page NNP 10_1101-2020_11_24_390039 132 32 10 10 CD 10_1101-2020_11_24_390039 132 33 of of IN 10_1101-2020_11_24_390039 132 34 21 21 CD 10_1101-2020_11_24_390039 132 35 Materials Materials NNPS 10_1101-2020_11_24_390039 132 36 and and CC 10_1101-2020_11_24_390039 132 37 Methods Methods NNP 10_1101-2020_11_24_390039 132 38 Ipom Ipom NNP 10_1101-2020_11_24_390039 132 39 - - HYPH 10_1101-2020_11_24_390039 132 40 F F NNP 10_1101-2020_11_24_390039 132 41 and and CC 10_1101-2020_11_24_390039 132 42 Antibodies Antibodies NNPS 10_1101-2020_11_24_390039 132 43 Ipom Ipom NNP 10_1101-2020_11_24_390039 132 44 - - HYPH 10_1101-2020_11_24_390039 132 45 F F NNP 10_1101-2020_11_24_390039 132 46 was be VBD 10_1101-2020_11_24_390039 132 47 synthesised synthesise VBN 10_1101-2020_11_24_390039 132 48 as as IN 10_1101-2020_11_24_390039 132 49 previously previously RB 10_1101-2020_11_24_390039 132 50 described describe VBN 10_1101-2020_11_24_390039 132 51 ( ( -LRB- 10_1101-2020_11_24_390039 132 52 Zong Zong NNP 10_1101-2020_11_24_390039 132 53 et et NNP 10_1101-2020_11_24_390039 132 54 al al NNP 10_1101-2020_11_24_390039 132 55 . . NNP 10_1101-2020_11_24_390039 132 56 , , , 10_1101-2020_11_24_390039 132 57 in in IN 10_1101-2020_11_24_390039 132 58 press press NN 10_1101-2020_11_24_390039 132 59 ) ) -RRB- 10_1101-2020_11_24_390039 132 60 . . . 10_1101-2020_11_24_390039 133 1 Antibodies antibody NNS 10_1101-2020_11_24_390039 133 2 used use VBD 10_1101-2020_11_24_390039 133 3 to to TO 10_1101-2020_11_24_390039 133 4 validate validate VB 10_1101-2020_11_24_390039 133 5 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 133 6 and/or and/or CC 10_1101-2020_11_24_390039 133 7 EMC EMC NNP 10_1101-2020_11_24_390039 133 8 subunit subunit NN 10_1101-2020_11_24_390039 133 9 depletions depletion NNS 10_1101-2020_11_24_390039 133 10 in in IN 10_1101-2020_11_24_390039 133 11 SP SP NNP 10_1101-2020_11_24_390039 133 12 cells cell NNS 10_1101-2020_11_24_390039 133 13 ( ( -LRB- 10_1101-2020_11_24_390039 133 14 Fig fig NN 10_1101-2020_11_24_390039 133 15 . . . 10_1101-2020_11_24_390039 134 1 S2 s2 NN 10_1101-2020_11_24_390039 134 2 ) ) -RRB- 10_1101-2020_11_24_390039 134 3 were be VBD 10_1101-2020_11_24_390039 134 4 purchased purchase VBN 10_1101-2020_11_24_390039 134 5 from from IN 10_1101-2020_11_24_390039 134 6 Santa Santa NNP 10_1101-2020_11_24_390039 134 7 Cruz Cruz NNP 10_1101-2020_11_24_390039 134 8 Biotechnology Biotechnology NNP 10_1101-2020_11_24_390039 134 9 ( ( -LRB- 10_1101-2020_11_24_390039 134 10 goat goat NNP 10_1101-2020_11_24_390039 134 11 polyclonal polyclonal JJ 10_1101-2020_11_24_390039 134 12 anti- anti- NNP 10_1101-2020_11_24_390039 134 13 LMNB1 LMNB1 '' 10_1101-2020_11_24_390039 134 14 ( ( -LRB- 10_1101-2020_11_24_390039 134 15 clone clone NN 10_1101-2020_11_24_390039 134 16 M-20 m-20 CD 10_1101-2020_11_24_390039 134 17 , , , 10_1101-2020_11_24_390039 134 18 sc-6217 sc-6217 NNP 10_1101-2020_11_24_390039 134 19 ) ) -RRB- 10_1101-2020_11_24_390039 134 20 , , , 10_1101-2020_11_24_390039 134 21 Bethyl Bethyl NNP 10_1101-2020_11_24_390039 134 22 Laboratories Laboratories NNP 10_1101-2020_11_24_390039 134 23 ( ( -LRB- 10_1101-2020_11_24_390039 134 24 rabbit rabbit NN 10_1101-2020_11_24_390039 134 25 polyclonal polyclonal JJ 10_1101-2020_11_24_390039 134 26 anti anti JJ 10_1101-2020_11_24_390039 134 27 - - JJ 10_1101-2020_11_24_390039 134 28 EMC5 emc5 JJ 10_1101-2020_11_24_390039 134 29 ( ( -LRB- 10_1101-2020_11_24_390039 134 30 A305 A305 NNP 10_1101-2020_11_24_390039 134 31 - - HYPH 10_1101-2020_11_24_390039 134 32 832-A 832-A NNP 10_1101-2020_11_24_390039 134 33 ) ) -RRB- 10_1101-2020_11_24_390039 134 34 ) ) -RRB- 10_1101-2020_11_24_390039 134 35 , , , 10_1101-2020_11_24_390039 134 36 Abcam Abcam NNP 10_1101-2020_11_24_390039 134 37 ( ( -LRB- 10_1101-2020_11_24_390039 134 38 rabbit rabbit NN 10_1101-2020_11_24_390039 134 39 polyclonal polyclonal JJ 10_1101-2020_11_24_390039 134 40 anti anti JJ 10_1101-2020_11_24_390039 134 41 - - NNP 10_1101-2020_11_24_390039 134 42 EMC6 EMC6 NNP 10_1101-2020_11_24_390039 134 43 , , , 10_1101-2020_11_24_390039 134 44 ( ( -LRB- 10_1101-2020_11_24_390039 134 45 ab84902 ab84902 FW 10_1101-2020_11_24_390039 134 46 ) ) -RRB- 10_1101-2020_11_24_390039 134 47 ) ) -RRB- 10_1101-2020_11_24_390039 134 48 , , , 10_1101-2020_11_24_390039 134 49 gifted gift VBN 10_1101-2020_11_24_390039 134 50 by by IN 10_1101-2020_11_24_390039 134 51 Sven Sven NNP 10_1101-2020_11_24_390039 134 52 Lang Lang NNP 10_1101-2020_11_24_390039 134 53 and and CC 10_1101-2020_11_24_390039 134 54 Richard Richard NNP 10_1101-2020_11_24_390039 134 55 Zimmermann Zimmermann NNP 10_1101-2020_11_24_390039 134 56 ( ( -LRB- 10_1101-2020_11_24_390039 134 57 University University NNP 10_1101-2020_11_24_390039 134 58 of of IN 10_1101-2020_11_24_390039 134 59 Saarland Saarland NNP 10_1101-2020_11_24_390039 134 60 , , , 10_1101-2020_11_24_390039 134 61 Homburg Homburg NNP 10_1101-2020_11_24_390039 134 62 , , , 10_1101-2020_11_24_390039 134 63 Germany Germany NNP 10_1101-2020_11_24_390039 134 64 , , , 10_1101-2020_11_24_390039 134 65 rabbit rabbit NN 10_1101-2020_11_24_390039 134 66 anti anti JJ 10_1101-2020_11_24_390039 134 67 - - JJ 10_1101-2020_11_24_390039 134 68 Sec61α sec61α JJ 10_1101-2020_11_24_390039 134 69 ) ) -RRB- 10_1101-2020_11_24_390039 134 70 or or CC 10_1101-2020_11_24_390039 134 71 as as RB 10_1101-2020_11_24_390039 134 72 previously previously RB 10_1101-2020_11_24_390039 134 73 described describe VBN 10_1101-2020_11_24_390039 134 74 ( ( -LRB- 10_1101-2020_11_24_390039 134 75 mouse mouse NN 10_1101-2020_11_24_390039 134 76 monoclonal monoclonal JJ 10_1101-2020_11_24_390039 134 77 anti anti NNP 10_1101-2020_11_24_390039 134 78 - - JJ 10_1101-2020_11_24_390039 134 79 OPG2 opg2 JJ 10_1101-2020_11_24_390039 134 80 tag tag NN 10_1101-2020_11_24_390039 134 81 ( ( -LRB- 10_1101-2020_11_24_390039 134 82 McKenna McKenna NNP 10_1101-2020_11_24_390039 134 83 et et NNP 10_1101-2020_11_24_390039 134 84 al al NNP 10_1101-2020_11_24_390039 134 85 . . NNP 10_1101-2020_11_24_390039 134 86 , , , 10_1101-2020_11_24_390039 134 87 2016 2016 CD 10_1101-2020_11_24_390039 134 88 ) ) -RRB- 10_1101-2020_11_24_390039 134 89 and and CC 10_1101-2020_11_24_390039 134 90 rabbit rabbit NN 10_1101-2020_11_24_390039 134 91 polyclonal polyclonal JJ 10_1101-2020_11_24_390039 134 92 anti anti JJ 10_1101-2020_11_24_390039 134 93 - - JJ 10_1101-2020_11_24_390039 134 94 OST48 ost48 JJ 10_1101-2020_11_24_390039 134 95 ( ( -LRB- 10_1101-2020_11_24_390039 134 96 Wilson Wilson NNP 10_1101-2020_11_24_390039 134 97 et et NNP 10_1101-2020_11_24_390039 134 98 al al NNP 10_1101-2020_11_24_390039 134 99 . . NNP 10_1101-2020_11_24_390039 134 100 , , , 10_1101-2020_11_24_390039 134 101 2007 2007 CD 10_1101-2020_11_24_390039 134 102 ) ) -RRB- 10_1101-2020_11_24_390039 134 103 . . . 10_1101-2020_11_24_390039 135 1 DNA DNA NNP 10_1101-2020_11_24_390039 135 2 constructs construct VBZ 10_1101-2020_11_24_390039 135 3 The the DT 10_1101-2020_11_24_390039 135 4 cDNA cdna NN 10_1101-2020_11_24_390039 135 5 for for IN 10_1101-2020_11_24_390039 135 6 human human JJ 10_1101-2020_11_24_390039 135 7 ACE2 ACE2 NNP 10_1101-2020_11_24_390039 135 8 ( ( -LRB- 10_1101-2020_11_24_390039 135 9 Uniprot Uniprot NNP 10_1101-2020_11_24_390039 135 10 : : : 10_1101-2020_11_24_390039 135 11 Q9BYF1 Q9BYF1 NNP 10_1101-2020_11_24_390039 135 12 ) ) -RRB- 10_1101-2020_11_24_390039 135 13 was be VBD 10_1101-2020_11_24_390039 135 14 purchased purchase VBN 10_1101-2020_11_24_390039 135 15 from from IN 10_1101-2020_11_24_390039 135 16 Sino Sino NNP 10_1101-2020_11_24_390039 135 17 Biological Biological NNP 10_1101-2020_11_24_390039 135 18 ( ( -LRB- 10_1101-2020_11_24_390039 135 19 HG10108-M HG10108-M NNP 10_1101-2020_11_24_390039 135 20 ) ) -RRB- 10_1101-2020_11_24_390039 135 21 . . . 10_1101-2020_11_24_390039 136 1 cDNAs cDNAs NNP 10_1101-2020_11_24_390039 136 2 encoding encode VBG 10_1101-2020_11_24_390039 136 3 the the DT 10_1101-2020_11_24_390039 136 4 SARS SARS NNP 10_1101-2020_11_24_390039 136 5 - - HYPH 10_1101-2020_11_24_390039 136 6 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 136 7 genes gene NNS 10_1101-2020_11_24_390039 136 8 for for IN 10_1101-2020_11_24_390039 136 9 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 136 10 , , , 10_1101-2020_11_24_390039 136 11 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 136 12 and and CC 10_1101-2020_11_24_390039 136 13 the the DT 10_1101-2020_11_24_390039 136 14 E E NNP 10_1101-2020_11_24_390039 136 15 M M NNP 10_1101-2020_11_24_390039 136 16 and and CC 10_1101-2020_11_24_390039 136 17 S S NNP 10_1101-2020_11_24_390039 136 18 proteins protein NNS 10_1101-2020_11_24_390039 136 19 ( ( -LRB- 10_1101-2020_11_24_390039 136 20 Uniprot Uniprot NNP 10_1101-2020_11_24_390039 136 21 : : : 10_1101-2020_11_24_390039 136 22 P0DTC6 p0dtc6 NN 10_1101-2020_11_24_390039 136 23 , , , 10_1101-2020_11_24_390039 136 24 P0DTC8 P0DTC8 NNP 10_1101-2020_11_24_390039 136 25 , , , 10_1101-2020_11_24_390039 136 26 P0DTC4 P0DTC4 NNP 10_1101-2020_11_24_390039 136 27 , , , 10_1101-2020_11_24_390039 136 28 P0DTC5 P0DTC5 NNP 10_1101-2020_11_24_390039 136 29 , , , 10_1101-2020_11_24_390039 136 30 P0DTC2 P0DTC2 NNP 10_1101-2020_11_24_390039 136 31 respectively respectively RB 10_1101-2020_11_24_390039 136 32 ) ) -RRB- 10_1101-2020_11_24_390039 136 33 were be VBD 10_1101-2020_11_24_390039 136 34 kindly kindly RB 10_1101-2020_11_24_390039 136 35 provided provide VBN 10_1101-2020_11_24_390039 136 36 by by IN 10_1101-2020_11_24_390039 136 37 Nevan Nevan NNP 10_1101-2020_11_24_390039 136 38 Krogan Krogan NNP 10_1101-2020_11_24_390039 136 39 ( ( -LRB- 10_1101-2020_11_24_390039 136 40 UCSF UCSF NNP 10_1101-2020_11_24_390039 136 41 , , , 10_1101-2020_11_24_390039 136 42 US US NNP 10_1101-2020_11_24_390039 136 43 ) ) -RRB- 10_1101-2020_11_24_390039 136 44 ( ( -LRB- 10_1101-2020_11_24_390039 136 45 Gordon Gordon NNP 10_1101-2020_11_24_390039 136 46 et et FW 10_1101-2020_11_24_390039 136 47 al al NNP 10_1101-2020_11_24_390039 136 48 . . . 10_1101-2020_11_24_390039 137 1 2020 2020 CD 10_1101-2020_11_24_390039 137 2 ) ) -RRB- 10_1101-2020_11_24_390039 137 3 , , , 10_1101-2020_11_24_390039 137 4 amplified amplify VBN 10_1101-2020_11_24_390039 137 5 by by IN 10_1101-2020_11_24_390039 137 6 PCR PCR NNP 10_1101-2020_11_24_390039 137 7 , , , 10_1101-2020_11_24_390039 137 8 subcloned subclone VBN 10_1101-2020_11_24_390039 137 9 into into IN 10_1101-2020_11_24_390039 137 10 the the DT 10_1101-2020_11_24_390039 137 11 pcDNA5 pcDNA5 NNP 10_1101-2020_11_24_390039 137 12 vector vector NN 10_1101-2020_11_24_390039 137 13 and and CC 10_1101-2020_11_24_390039 137 14 constructs construct NNS 10_1101-2020_11_24_390039 137 15 validated validate VBN 10_1101-2020_11_24_390039 137 16 by by IN 10_1101-2020_11_24_390039 137 17 DNA DNA NNP 10_1101-2020_11_24_390039 137 18 sequencing sequencing NN 10_1101-2020_11_24_390039 137 19 ( ( -LRB- 10_1101-2020_11_24_390039 137 20 GATC GATC NNP 10_1101-2020_11_24_390039 137 21 , , , 10_1101-2020_11_24_390039 137 22 Eurofins Eurofins NNP 10_1101-2020_11_24_390039 137 23 Genomics Genomics NNP 10_1101-2020_11_24_390039 137 24 ) ) -RRB- 10_1101-2020_11_24_390039 137 25 . . . 10_1101-2020_11_24_390039 138 1 ORF6-OPG2 ORF6-OPG2 NNP 10_1101-2020_11_24_390039 138 2 , , , 10_1101-2020_11_24_390039 138 3 ORF8-OPG2 ORF8-OPG2 NNP 10_1101-2020_11_24_390039 138 4 , , , 10_1101-2020_11_24_390039 138 5 M M NNP 10_1101-2020_11_24_390039 138 6 - - HYPH 10_1101-2020_11_24_390039 138 7 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 138 8 and and CC 10_1101-2020_11_24_390039 138 9 S S NNP 10_1101-2020_11_24_390039 138 10 - - HYPH 10_1101-2020_11_24_390039 138 11 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 138 12 were be VBD 10_1101-2020_11_24_390039 138 13 generated generate VBN 10_1101-2020_11_24_390039 138 14 by by IN 10_1101-2020_11_24_390039 138 15 inserting insert VBG 10_1101-2020_11_24_390039 138 16 the the DT 10_1101-2020_11_24_390039 138 17 respective respective JJ 10_1101-2020_11_24_390039 138 18 cDNAs cdnas NN 10_1101-2020_11_24_390039 138 19 in in IN 10_1101-2020_11_24_390039 138 20 frame frame NN 10_1101-2020_11_24_390039 138 21 between between IN 10_1101-2020_11_24_390039 138 22 NheI NheI NNP 10_1101-2020_11_24_390039 138 23 and and CC 10_1101-2020_11_24_390039 138 24 AflII AflII NNP 10_1101-2020_11_24_390039 138 25 sites site NNS 10_1101-2020_11_24_390039 138 26 of of IN 10_1101-2020_11_24_390039 138 27 a a DT 10_1101-2020_11_24_390039 138 28 pcDNA5 pcDNA5 NNP 10_1101-2020_11_24_390039 138 29 / / SYM 10_1101-2020_11_24_390039 138 30 FRT frt NN 10_1101-2020_11_24_390039 138 31 / / SYM 10_1101-2020_11_24_390039 138 32 V5- V5- NNP 10_1101-2020_11_24_390039 138 33 His -PRON- PRP$ 10_1101-2020_11_24_390039 138 34 vector vector NN 10_1101-2020_11_24_390039 138 35 ( ( -LRB- 10_1101-2020_11_24_390039 138 36 Invitrogen Invitrogen NNP 10_1101-2020_11_24_390039 138 37 ) ) -RRB- 10_1101-2020_11_24_390039 138 38 containing contain VBG 10_1101-2020_11_24_390039 138 39 a a DT 10_1101-2020_11_24_390039 138 40 C C NNP 10_1101-2020_11_24_390039 138 41 - - HYPH 10_1101-2020_11_24_390039 138 42 terminal terminal JJ 10_1101-2020_11_24_390039 138 43 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 138 44 tag tag NN 10_1101-2020_11_24_390039 138 45 ( ( -LRB- 10_1101-2020_11_24_390039 138 46 MNGTEGPNFYVPFSNKTG MNGTEGPNFYVPFSNKTG NNP 10_1101-2020_11_24_390039 138 47 ) ) -RRB- 10_1101-2020_11_24_390039 138 48 . . . 10_1101-2020_11_24_390039 139 1 OPG2-E OPG2-E NNP 10_1101-2020_11_24_390039 139 2 was be VBD 10_1101-2020_11_24_390039 139 3 generated generate VBN 10_1101-2020_11_24_390039 139 4 by by IN 10_1101-2020_11_24_390039 139 5 cloning clone VBG 10_1101-2020_11_24_390039 139 6 the the DT 10_1101-2020_11_24_390039 139 7 cDNA cdna NN 10_1101-2020_11_24_390039 139 8 encoding encode VBG 10_1101-2020_11_24_390039 139 9 the the DT 10_1101-2020_11_24_390039 139 10 E e NN 10_1101-2020_11_24_390039 139 11 - - NN 10_1101-2020_11_24_390039 139 12 protein protein NN 10_1101-2020_11_24_390039 139 13 into into IN 10_1101-2020_11_24_390039 139 14 the the DT 10_1101-2020_11_24_390039 139 15 same same JJ 10_1101-2020_11_24_390039 139 16 pcDNA5-OPG2 pcDNA5-OPG2 NNP 10_1101-2020_11_24_390039 139 17 vector vector NN 10_1101-2020_11_24_390039 139 18 using use VBG 10_1101-2020_11_24_390039 139 19 the the DT 10_1101-2020_11_24_390039 139 20 KpnI KpnI NNP 10_1101-2020_11_24_390039 139 21 and and CC 10_1101-2020_11_24_390039 139 22 BamHI BamHI NNP 10_1101-2020_11_24_390039 139 23 sites site NNS 10_1101-2020_11_24_390039 139 24 and and CC 10_1101-2020_11_24_390039 139 25 deleting delete VBG 10_1101-2020_11_24_390039 139 26 the the DT 10_1101-2020_11_24_390039 139 27 stop stop NN 10_1101-2020_11_24_390039 139 28 codon codon NN 10_1101-2020_11_24_390039 139 29 after after IN 10_1101-2020_11_24_390039 139 30 the the DT 10_1101-2020_11_24_390039 139 31 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 139 32 tag tag NN 10_1101-2020_11_24_390039 139 33 by by IN 10_1101-2020_11_24_390039 139 34 site site NN 10_1101-2020_11_24_390039 139 35 - - HYPH 10_1101-2020_11_24_390039 139 36 directed direct VBN 10_1101-2020_11_24_390039 139 37 mutagenesis mutagenesis NN 10_1101-2020_11_24_390039 139 38 ( ( -LRB- 10_1101-2020_11_24_390039 139 39 Stratagene Stratagene NNP 10_1101-2020_11_24_390039 139 40 QuikChange QuikChange NNP 10_1101-2020_11_24_390039 139 41 , , , 10_1101-2020_11_24_390039 139 42 Agilent Agilent NNP 10_1101-2020_11_24_390039 139 43 Technologies Technologies NNP 10_1101-2020_11_24_390039 139 44 ) ) -RRB- 10_1101-2020_11_24_390039 139 45 . . . 10_1101-2020_11_24_390039 140 1 The the DT 10_1101-2020_11_24_390039 140 2 N n CD 10_1101-2020_11_24_390039 140 3 - - HYPH 10_1101-2020_11_24_390039 140 4 terminal terminal NN 10_1101-2020_11_24_390039 140 5 OPG2-tag OPG2-tag NNP 10_1101-2020_11_24_390039 140 6 of of IN 10_1101-2020_11_24_390039 140 7 OPG2-ORF6-OPG2 OPG2-ORF6-OPG2 NNP 10_1101-2020_11_24_390039 140 8 was be VBD 10_1101-2020_11_24_390039 140 9 inserted insert VBN 10_1101-2020_11_24_390039 140 10 by by IN 10_1101-2020_11_24_390039 140 11 site site NN 10_1101-2020_11_24_390039 140 12 - - HYPH 10_1101-2020_11_24_390039 140 13 directed direct VBN 10_1101-2020_11_24_390039 140 14 mutagenesis mutagenesis NN 10_1101-2020_11_24_390039 140 15 of of IN 10_1101-2020_11_24_390039 140 16 ORF6-OPG2 ORF6-OPG2 NNP 10_1101-2020_11_24_390039 140 17 using use VBG 10_1101-2020_11_24_390039 140 18 the the DT 10_1101-2020_11_24_390039 140 19 relevant relevant JJ 10_1101-2020_11_24_390039 140 20 forward forward NN 10_1101-2020_11_24_390039 140 21 and and CC 10_1101-2020_11_24_390039 140 22 reverse reverse VB 10_1101-2020_11_24_390039 140 23 primers primer NNS 10_1101-2020_11_24_390039 140 24 ( ( -LRB- 10_1101-2020_11_24_390039 140 25 Integrated Integrated NNP 10_1101-2020_11_24_390039 140 26 DNA DNA NNP 10_1101-2020_11_24_390039 140 27 Technologies Technologies NNP 10_1101-2020_11_24_390039 140 28 ) ) -RRB- 10_1101-2020_11_24_390039 140 29 . . . 10_1101-2020_11_24_390039 141 1 Linear linear JJ 10_1101-2020_11_24_390039 141 2 DNA dna NN 10_1101-2020_11_24_390039 141 3 templates template NNS 10_1101-2020_11_24_390039 141 4 were be VBD 10_1101-2020_11_24_390039 141 5 generated generate VBN 10_1101-2020_11_24_390039 141 6 by by IN 10_1101-2020_11_24_390039 141 7 PCR PCR NNP 10_1101-2020_11_24_390039 141 8 and and CC 10_1101-2020_11_24_390039 141 9 mRNA mRNA NNP 10_1101-2020_11_24_390039 141 10 transcribed transcribed NNP 10_1101-2020_11_24_390039 141 11 using use VBG 10_1101-2020_11_24_390039 141 12 T7 T7 NNP 10_1101-2020_11_24_390039 141 13 polymerase polymerase NN 10_1101-2020_11_24_390039 141 14 . . . 10_1101-2020_11_24_390039 142 1 siRNA siRNA NNP 10_1101-2020_11_24_390039 142 2 - - HYPH 10_1101-2020_11_24_390039 142 3 mediated mediate VBN 10_1101-2020_11_24_390039 142 4 knockdown knockdown NN 10_1101-2020_11_24_390039 142 5 and and CC 10_1101-2020_11_24_390039 142 6 SP SP NNP 10_1101-2020_11_24_390039 142 7 cell cell NN 10_1101-2020_11_24_390039 142 8 preparation preparation NN 10_1101-2020_11_24_390039 142 9 HeLa HeLa NNP 10_1101-2020_11_24_390039 142 10 cells cell NNS 10_1101-2020_11_24_390039 142 11 ( ( -LRB- 10_1101-2020_11_24_390039 142 12 human human JJ 10_1101-2020_11_24_390039 142 13 epithelial epithelial JJ 10_1101-2020_11_24_390039 142 14 cervix cervix NN 10_1101-2020_11_24_390039 142 15 carcinoma carcinoma NN 10_1101-2020_11_24_390039 142 16 cells cell NNS 10_1101-2020_11_24_390039 142 17 ) ) -RRB- 10_1101-2020_11_24_390039 142 18 were be VBD 10_1101-2020_11_24_390039 142 19 cultured culture VBN 10_1101-2020_11_24_390039 142 20 in in IN 10_1101-2020_11_24_390039 142 21 DMEM DMEM NNP 10_1101-2020_11_24_390039 142 22 supplemented supplement VBN 10_1101-2020_11_24_390039 142 23 with with IN 10_1101-2020_11_24_390039 142 24 10 10 CD 10_1101-2020_11_24_390039 142 25 % % NN 10_1101-2020_11_24_390039 142 26 ( ( -LRB- 10_1101-2020_11_24_390039 142 27 v v NN 10_1101-2020_11_24_390039 142 28 / / SYM 10_1101-2020_11_24_390039 142 29 v v NN 10_1101-2020_11_24_390039 142 30 ) ) -RRB- 10_1101-2020_11_24_390039 142 31 FBS FBS NNP 10_1101-2020_11_24_390039 142 32 and and CC 10_1101-2020_11_24_390039 142 33 maintained maintain VBD 10_1101-2020_11_24_390039 142 34 in in IN 10_1101-2020_11_24_390039 142 35 a a DT 10_1101-2020_11_24_390039 142 36 5 5 CD 10_1101-2020_11_24_390039 142 37 % % NN 10_1101-2020_11_24_390039 142 38 CO2 CO2 NNP 10_1101-2020_11_24_390039 142 39 humidified humidify VBD 10_1101-2020_11_24_390039 142 40 incubator incubator NN 10_1101-2020_11_24_390039 142 41 at at IN 10_1101-2020_11_24_390039 142 42 37 37 CD 10_1101-2020_11_24_390039 142 43 ° ° NNS 10_1101-2020_11_24_390039 142 44 C C NNP 10_1101-2020_11_24_390039 142 45 . . . 10_1101-2020_11_24_390039 143 1 Knockdown Knockdown VBN 10_1101-2020_11_24_390039 143 2 of of IN 10_1101-2020_11_24_390039 143 3 target target NN 10_1101-2020_11_24_390039 143 4 genes gene NNS 10_1101-2020_11_24_390039 143 5 were be VBD 10_1101-2020_11_24_390039 143 6 performed perform VBN 10_1101-2020_11_24_390039 143 7 as as IN 10_1101-2020_11_24_390039 143 8 previously previously RB 10_1101-2020_11_24_390039 143 9 .CC .CC : 10_1101-2020_11_24_390039 143 10 - - : 10_1101-2020_11_24_390039 143 11 BY by IN 10_1101-2020_11_24_390039 143 12 - - HYPH 10_1101-2020_11_24_390039 143 13 ND ND NNP 10_1101-2020_11_24_390039 143 14 4.0 4.0 CD 10_1101-2020_11_24_390039 143 15 International international JJ 10_1101-2020_11_24_390039 143 16 licensemade licensemade NN 10_1101-2020_11_24_390039 143 17 available available JJ 10_1101-2020_11_24_390039 143 18 under under IN 10_1101-2020_11_24_390039 143 19 a a DT 10_1101-2020_11_24_390039 143 20 ( ( -LRB- 10_1101-2020_11_24_390039 143 21 which which WDT 10_1101-2020_11_24_390039 143 22 was be VBD 10_1101-2020_11_24_390039 143 23 not not RB 10_1101-2020_11_24_390039 143 24 certified certify VBN 10_1101-2020_11_24_390039 143 25 by by IN 10_1101-2020_11_24_390039 143 26 peer peer NN 10_1101-2020_11_24_390039 143 27 review review NN 10_1101-2020_11_24_390039 143 28 ) ) -RRB- 10_1101-2020_11_24_390039 143 29 is be VBZ 10_1101-2020_11_24_390039 143 30 the the DT 10_1101-2020_11_24_390039 143 31 author author NN 10_1101-2020_11_24_390039 143 32 / / SYM 10_1101-2020_11_24_390039 143 33 funder funder NN 10_1101-2020_11_24_390039 143 34 , , , 10_1101-2020_11_24_390039 143 35 who who WP 10_1101-2020_11_24_390039 143 36 has have VBZ 10_1101-2020_11_24_390039 143 37 granted grant VBN 10_1101-2020_11_24_390039 143 38 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 143 39 a a DT 10_1101-2020_11_24_390039 143 40 license license NN 10_1101-2020_11_24_390039 143 41 to to TO 10_1101-2020_11_24_390039 143 42 display display VB 10_1101-2020_11_24_390039 143 43 the the DT 10_1101-2020_11_24_390039 143 44 preprint preprint NN 10_1101-2020_11_24_390039 143 45 in in IN 10_1101-2020_11_24_390039 143 46 perpetuity perpetuity NN 10_1101-2020_11_24_390039 143 47 . . . 10_1101-2020_11_24_390039 144 1 It -PRON- PRP 10_1101-2020_11_24_390039 144 2 is be VBZ 10_1101-2020_11_24_390039 144 3 The the DT 10_1101-2020_11_24_390039 144 4 copyright copyright NN 10_1101-2020_11_24_390039 144 5 holder holder NN 10_1101-2020_11_24_390039 144 6 for for IN 10_1101-2020_11_24_390039 144 7 this this DT 10_1101-2020_11_24_390039 144 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 144 9 version version NN 10_1101-2020_11_24_390039 144 10 posted post VBD 10_1101-2020_11_24_390039 144 11 January January NNP 10_1101-2020_11_24_390039 144 12 5 5 CD 10_1101-2020_11_24_390039 144 13 , , , 10_1101-2020_11_24_390039 144 14 2021 2021 CD 10_1101-2020_11_24_390039 144 15 . . . 10_1101-2020_11_24_390039 144 16 ; ; : 10_1101-2020_11_24_390039 144 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 144 18 : : : 10_1101-2020_11_24_390039 144 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 144 20 preprint preprint JJ 10_1101-2020_11_24_390039 144 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 144 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 144 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 144 24 - - HYPH 10_1101-2020_11_24_390039 144 25 F F NNP 10_1101-2020_11_24_390039 144 26 as as IN 10_1101-2020_11_24_390039 144 27 a a DT 10_1101-2020_11_24_390039 144 28 potential potential JJ 10_1101-2020_11_24_390039 144 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 144 30 agent agent NN 10_1101-2020_11_24_390039 144 31 Page Page NNP 10_1101-2020_11_24_390039 144 32 11 11 CD 10_1101-2020_11_24_390039 144 33 of of IN 10_1101-2020_11_24_390039 144 34 21 21 CD 10_1101-2020_11_24_390039 144 35 described describe VBN 10_1101-2020_11_24_390039 144 36 ( ( -LRB- 10_1101-2020_11_24_390039 144 37 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 144 38 et et NNP 10_1101-2020_11_24_390039 144 39 al al NNP 10_1101-2020_11_24_390039 144 40 . . NNP 10_1101-2020_11_24_390039 144 41 , , , 10_1101-2020_11_24_390039 144 42 2020 2020 CD 10_1101-2020_11_24_390039 144 43 submitted submit VBN 10_1101-2020_11_24_390039 144 44 ) ) -RRB- 10_1101-2020_11_24_390039 144 45 using use VBG 10_1101-2020_11_24_390039 144 46 20 20 CD 10_1101-2020_11_24_390039 144 47 nM nM NNP 10_1101-2020_11_24_390039 144 48 ( ( -LRB- 10_1101-2020_11_24_390039 144 49 final final JJ 10_1101-2020_11_24_390039 144 50 concentration concentration NN 10_1101-2020_11_24_390039 144 51 ) ) -RRB- 10_1101-2020_11_24_390039 144 52 of of IN 10_1101-2020_11_24_390039 144 53 either either DT 10_1101-2020_11_24_390039 144 54 control control NN 10_1101-2020_11_24_390039 144 55 siRNA siRNA . 10_1101-2020_11_24_390039 144 56 ( ( -LRB- 10_1101-2020_11_24_390039 144 57 ON ON NNP 10_1101-2020_11_24_390039 144 58 - - HYPH 10_1101-2020_11_24_390039 144 59 TARGETplus TARGETplus NNP 10_1101-2020_11_24_390039 144 60 Non non JJ 10_1101-2020_11_24_390039 144 61 - - JJ 10_1101-2020_11_24_390039 144 62 targeting targeting JJ 10_1101-2020_11_24_390039 144 63 control control NN 10_1101-2020_11_24_390039 144 64 pool pool NN 10_1101-2020_11_24_390039 144 65 ; ; : 10_1101-2020_11_24_390039 144 66 Dharmacon Dharmacon NNP 10_1101-2020_11_24_390039 144 67 ) ) -RRB- 10_1101-2020_11_24_390039 144 68 , , , 10_1101-2020_11_24_390039 144 69 SEC61A1 sec61a1 NN 10_1101-2020_11_24_390039 144 70 siRNA siRNA . 10_1101-2020_11_24_390039 144 71 ( ( -LRB- 10_1101-2020_11_24_390039 144 72 Sec61α Sec61α NNP 10_1101-2020_11_24_390039 144 73 - - HYPH 10_1101-2020_11_24_390039 144 74 kd kd NNP 10_1101-2020_11_24_390039 144 75 , , , 10_1101-2020_11_24_390039 144 76 GE GE NNP 10_1101-2020_11_24_390039 144 77 Healthcare Healthcare NNP 10_1101-2020_11_24_390039 144 78 , , , 10_1101-2020_11_24_390039 144 79 sequence sequence NN 10_1101-2020_11_24_390039 144 80 AACACUGAAAUGUCUACGUUUUU AACACUGAAAUGUCUACGUUUUU NNP 10_1101-2020_11_24_390039 144 81 ) ) -RRB- 10_1101-2020_11_24_390039 144 82 , , , 10_1101-2020_11_24_390039 144 83 MMGT1 MMGT1 '' 10_1101-2020_11_24_390039 144 84 siRNA siRNA NNP 10_1101-2020_11_24_390039 144 85 ( ( -LRB- 10_1101-2020_11_24_390039 144 86 EMC5-kd EMC5-kd NNP 10_1101-2020_11_24_390039 144 87 , , , 10_1101-2020_11_24_390039 144 88 ThermoFisher ThermoFisher NNP 10_1101-2020_11_24_390039 144 89 Scientific Scientific NNP 10_1101-2020_11_24_390039 144 90 , , , 10_1101-2020_11_24_390039 144 91 s41129 s41129 JJ 10_1101-2020_11_24_390039 144 92 ) ) -RRB- 10_1101-2020_11_24_390039 144 93 and and CC 10_1101-2020_11_24_390039 144 94 INTERFERin INTERFERin NNP 10_1101-2020_11_24_390039 144 95 ( ( -LRB- 10_1101-2020_11_24_390039 144 96 Polyplus Polyplus NNP 10_1101-2020_11_24_390039 144 97 , , , 10_1101-2020_11_24_390039 144 98 409 409 CD 10_1101-2020_11_24_390039 144 99 - - SYM 10_1101-2020_11_24_390039 144 100 10 10 CD 10_1101-2020_11_24_390039 144 101 ) ) -RRB- 10_1101-2020_11_24_390039 144 102 as as IN 10_1101-2020_11_24_390039 144 103 described describe VBN 10_1101-2020_11_24_390039 144 104 by by IN 10_1101-2020_11_24_390039 144 105 the the DT 10_1101-2020_11_24_390039 144 106 manufacturer manufacturer NN 10_1101-2020_11_24_390039 144 107 . . . 10_1101-2020_11_24_390039 145 1 96 96 CD 10_1101-2020_11_24_390039 145 2 h h NN 10_1101-2020_11_24_390039 145 3 post post JJ 10_1101-2020_11_24_390039 145 4 - - JJ 10_1101-2020_11_24_390039 145 5 initial initial JJ 10_1101-2020_11_24_390039 145 6 transfection transfection NN 10_1101-2020_11_24_390039 145 7 , , , 10_1101-2020_11_24_390039 145 8 cells cell NNS 10_1101-2020_11_24_390039 145 9 were be VBD 10_1101-2020_11_24_390039 145 10 semi semi RB 10_1101-2020_11_24_390039 145 11 - - JJ 10_1101-2020_11_24_390039 145 12 permeabilsed permeabilsed JJ 10_1101-2020_11_24_390039 145 13 using use VBG 10_1101-2020_11_24_390039 145 14 80 80 CD 10_1101-2020_11_24_390039 145 15 μg μg CD 10_1101-2020_11_24_390039 145 16 / / SYM 10_1101-2020_11_24_390039 145 17 mL mL NNP 10_1101-2020_11_24_390039 145 18 high high JJ 10_1101-2020_11_24_390039 145 19 purity purity NN 10_1101-2020_11_24_390039 145 20 digitonin digitonin NN 10_1101-2020_11_24_390039 145 21 ( ( -LRB- 10_1101-2020_11_24_390039 145 22 Calbiochem Calbiochem NNP 10_1101-2020_11_24_390039 145 23 ) ) -RRB- 10_1101-2020_11_24_390039 145 24 and and CC 10_1101-2020_11_24_390039 145 25 treated treat VBN 10_1101-2020_11_24_390039 145 26 with with IN 10_1101-2020_11_24_390039 145 27 0.2 0.2 CD 10_1101-2020_11_24_390039 145 28 U U NNP 10_1101-2020_11_24_390039 145 29 Nuclease Nuclease NNP 10_1101-2020_11_24_390039 145 30 S7 S7 NNP 10_1101-2020_11_24_390039 145 31 Micrococcal Micrococcal NNP 10_1101-2020_11_24_390039 145 32 nuclease nuclease NN 10_1101-2020_11_24_390039 145 33 from from IN 10_1101-2020_11_24_390039 145 34 Staphylococcus Staphylococcus NNP 10_1101-2020_11_24_390039 145 35 aureus aureus NNP 10_1101-2020_11_24_390039 145 36 ( ( -LRB- 10_1101-2020_11_24_390039 145 37 Sigma Sigma NNP 10_1101-2020_11_24_390039 145 38 - - HYPH 10_1101-2020_11_24_390039 145 39 Aldrich Aldrich NNP 10_1101-2020_11_24_390039 145 40 , , , 10_1101-2020_11_24_390039 145 41 10107921001 10107921001 CD 10_1101-2020_11_24_390039 145 42 ) ) -RRB- 10_1101-2020_11_24_390039 145 43 as as IN 10_1101-2020_11_24_390039 145 44 previously previously RB 10_1101-2020_11_24_390039 145 45 described describe VBN 10_1101-2020_11_24_390039 145 46 ( ( -LRB- 10_1101-2020_11_24_390039 145 47 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 145 48 et et NNP 10_1101-2020_11_24_390039 145 49 al al NNP 10_1101-2020_11_24_390039 145 50 . . NNP 10_1101-2020_11_24_390039 145 51 , , , 10_1101-2020_11_24_390039 145 52 2020 2020 CD 10_1101-2020_11_24_390039 145 53 submitted submit VBD 10_1101-2020_11_24_390039 145 54 ; ; : 10_1101-2020_11_24_390039 145 55 Wilson Wilson NNP 10_1101-2020_11_24_390039 145 56 et et NNP 10_1101-2020_11_24_390039 145 57 al al NNP 10_1101-2020_11_24_390039 145 58 . . NNP 10_1101-2020_11_24_390039 145 59 , , , 10_1101-2020_11_24_390039 145 60 2007 2007 CD 10_1101-2020_11_24_390039 145 61 ) ) -RRB- 10_1101-2020_11_24_390039 145 62 . . . 10_1101-2020_11_24_390039 146 1 SP SP NNP 10_1101-2020_11_24_390039 146 2 cells cell NNS 10_1101-2020_11_24_390039 146 3 lacking lack VBG 10_1101-2020_11_24_390039 146 4 endogenous endogenous JJ 10_1101-2020_11_24_390039 146 5 mRNA mRNA NNS 10_1101-2020_11_24_390039 146 6 were be VBD 10_1101-2020_11_24_390039 146 7 resuspended resuspend VBN 10_1101-2020_11_24_390039 146 8 ( ( -LRB- 10_1101-2020_11_24_390039 146 9 3x106 3x106 CD 10_1101-2020_11_24_390039 146 10 SP SP NNP 10_1101-2020_11_24_390039 146 11 cells cell NNS 10_1101-2020_11_24_390039 146 12 / / SYM 10_1101-2020_11_24_390039 146 13 mL mL NNP 10_1101-2020_11_24_390039 146 14 as as IN 10_1101-2020_11_24_390039 146 15 determined determine VBN 10_1101-2020_11_24_390039 146 16 by by IN 10_1101-2020_11_24_390039 146 17 trypan trypan NN 10_1101-2020_11_24_390039 146 18 blue blue NNP 10_1101-2020_11_24_390039 146 19 ( ( -LRB- 10_1101-2020_11_24_390039 146 20 Sigma Sigma NNP 10_1101-2020_11_24_390039 146 21 - - HYPH 10_1101-2020_11_24_390039 146 22 Aldrich Aldrich NNP 10_1101-2020_11_24_390039 146 23 , , , 10_1101-2020_11_24_390039 146 24 T8154 T8154 NNP 10_1101-2020_11_24_390039 146 25 ) ) -RRB- 10_1101-2020_11_24_390039 146 26 staining stain VBG 10_1101-2020_11_24_390039 146 27 ) ) -RRB- 10_1101-2020_11_24_390039 146 28 in in IN 10_1101-2020_11_24_390039 146 29 KHM KHM NNP 10_1101-2020_11_24_390039 146 30 buffer buffer NN 10_1101-2020_11_24_390039 146 31 ( ( -LRB- 10_1101-2020_11_24_390039 146 32 110 110 CD 10_1101-2020_11_24_390039 146 33 mM mm NN 10_1101-2020_11_24_390039 146 34 KOAc KOAc NNS 10_1101-2020_11_24_390039 146 35 , , , 10_1101-2020_11_24_390039 146 36 2 2 CD 10_1101-2020_11_24_390039 146 37 mM mm NN 10_1101-2020_11_24_390039 146 38 Mg(OAc)2 mg(oac)2 NN 10_1101-2020_11_24_390039 146 39 , , , 10_1101-2020_11_24_390039 146 40 20 20 CD 10_1101-2020_11_24_390039 146 41 mM mM NNP 10_1101-2020_11_24_390039 146 42 HEPES HEPES NNP 10_1101-2020_11_24_390039 146 43 - - HYPH 10_1101-2020_11_24_390039 146 44 KOH KOH NNP 10_1101-2020_11_24_390039 146 45 pH pH NNP 10_1101-2020_11_24_390039 146 46 7.2 7.2 CD 10_1101-2020_11_24_390039 146 47 ) ) -RRB- 10_1101-2020_11_24_390039 146 48 prior prior RB 10_1101-2020_11_24_390039 146 49 to to IN 10_1101-2020_11_24_390039 146 50 analysis analysis NN 10_1101-2020_11_24_390039 146 51 by by IN 10_1101-2020_11_24_390039 146 52 western western JJ 10_1101-2020_11_24_390039 146 53 blot blot NN 10_1101-2020_11_24_390039 146 54 , , , 10_1101-2020_11_24_390039 146 55 or or CC 10_1101-2020_11_24_390039 146 56 inclusion inclusion NN 10_1101-2020_11_24_390039 146 57 in in IN 10_1101-2020_11_24_390039 146 58 translation translation NN 10_1101-2020_11_24_390039 146 59 master master NN 10_1101-2020_11_24_390039 146 60 mixes mix NNS 10_1101-2020_11_24_390039 146 61 such such JJ 10_1101-2020_11_24_390039 146 62 that that IN 10_1101-2020_11_24_390039 146 63 each each DT 10_1101-2020_11_24_390039 146 64 translation translation NN 10_1101-2020_11_24_390039 146 65 reaction reaction NN 10_1101-2020_11_24_390039 146 66 contained contain VBD 10_1101-2020_11_24_390039 146 67 2x105 2x105 CD 10_1101-2020_11_24_390039 146 68 cells cell NNS 10_1101-2020_11_24_390039 146 69 / / SYM 10_1101-2020_11_24_390039 146 70 mL. ml. NN 10_1101-2020_11_24_390039 147 1 In in IN 10_1101-2020_11_24_390039 147 2 vitro vitro FW 10_1101-2020_11_24_390039 147 3 ER ER NNP 10_1101-2020_11_24_390039 147 4 import import NN 10_1101-2020_11_24_390039 147 5 assays assays RB 10_1101-2020_11_24_390039 147 6 Standard standard JJ 10_1101-2020_11_24_390039 147 7 translation translation NN 10_1101-2020_11_24_390039 147 8 and and CC 10_1101-2020_11_24_390039 147 9 membrane membrane NN 10_1101-2020_11_24_390039 147 10 translocation translocation NN 10_1101-2020_11_24_390039 147 11 / / SYM 10_1101-2020_11_24_390039 147 12 insertion insertion NN 10_1101-2020_11_24_390039 147 13 assays assays RB 10_1101-2020_11_24_390039 147 14 , , , 10_1101-2020_11_24_390039 147 15 supplemented supplement VBN 10_1101-2020_11_24_390039 147 16 with with IN 10_1101-2020_11_24_390039 147 17 nuclease nuclease NN 10_1101-2020_11_24_390039 147 18 - - HYPH 10_1101-2020_11_24_390039 147 19 treated treat VBN 10_1101-2020_11_24_390039 147 20 canine canine NN 10_1101-2020_11_24_390039 147 21 pancreatic pancreatic JJ 10_1101-2020_11_24_390039 147 22 microsomes microsome NNS 10_1101-2020_11_24_390039 147 23 ( ( -LRB- 10_1101-2020_11_24_390039 147 24 from from IN 10_1101-2020_11_24_390039 147 25 stock stock NN 10_1101-2020_11_24_390039 147 26 with with IN 10_1101-2020_11_24_390039 147 27 OD280 OD280 NNP 10_1101-2020_11_24_390039 147 28 = = SYM 10_1101-2020_11_24_390039 147 29 44 44 CD 10_1101-2020_11_24_390039 147 30 / / SYM 10_1101-2020_11_24_390039 147 31 mL ml NN 10_1101-2020_11_24_390039 147 32 ) ) -RRB- 10_1101-2020_11_24_390039 147 33 or or CC 10_1101-2020_11_24_390039 147 34 siRNA sirna RB 10_1101-2020_11_24_390039 147 35 - - HYPH 10_1101-2020_11_24_390039 147 36 treated treat VBN 10_1101-2020_11_24_390039 147 37 SP SP NNP 10_1101-2020_11_24_390039 147 38 HeLa HeLa NNP 10_1101-2020_11_24_390039 147 39 cells cell NNS 10_1101-2020_11_24_390039 147 40 , , , 10_1101-2020_11_24_390039 147 41 were be VBD 10_1101-2020_11_24_390039 147 42 performed perform VBN 10_1101-2020_11_24_390039 147 43 in in IN 10_1101-2020_11_24_390039 147 44 nuclease nuclease NN 10_1101-2020_11_24_390039 147 45 - - HYPH 10_1101-2020_11_24_390039 147 46 treated treat VBN 10_1101-2020_11_24_390039 147 47 rabbit rabbit NN 10_1101-2020_11_24_390039 147 48 reticulocyte reticulocyte NN 10_1101-2020_11_24_390039 147 49 lysate lysate NN 10_1101-2020_11_24_390039 147 50 ( ( -LRB- 10_1101-2020_11_24_390039 147 51 Promega Promega NNP 10_1101-2020_11_24_390039 147 52 ) ) -RRB- 10_1101-2020_11_24_390039 147 53 as as IN 10_1101-2020_11_24_390039 147 54 previously previously RB 10_1101-2020_11_24_390039 147 55 described describe VBN 10_1101-2020_11_24_390039 147 56 ( ( -LRB- 10_1101-2020_11_24_390039 147 57 Zong Zong NNP 10_1101-2020_11_24_390039 147 58 et et NNP 10_1101-2020_11_24_390039 147 59 al al NNP 10_1101-2020_11_24_390039 147 60 . . NNP 10_1101-2020_11_24_390039 147 61 , , , 10_1101-2020_11_24_390039 147 62 2019 2019 CD 10_1101-2020_11_24_390039 147 63 ; ; : 10_1101-2020_11_24_390039 147 64 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 147 65 et et NNP 10_1101-2020_11_24_390039 147 66 al al NNP 10_1101-2020_11_24_390039 147 67 . . NNP 10_1101-2020_11_24_390039 147 68 , , , 10_1101-2020_11_24_390039 147 69 2020 2020 CD 10_1101-2020_11_24_390039 147 70 submitted submit VBN 10_1101-2020_11_24_390039 147 71 ) ) -RRB- 10_1101-2020_11_24_390039 147 72 : : : 10_1101-2020_11_24_390039 147 73 namely namely RB 10_1101-2020_11_24_390039 147 74 in in IN 10_1101-2020_11_24_390039 147 75 the the DT 10_1101-2020_11_24_390039 147 76 presence presence NN 10_1101-2020_11_24_390039 147 77 of of IN 10_1101-2020_11_24_390039 147 78 EasyTag EasyTag NNP 10_1101-2020_11_24_390039 147 79 EXPRESS EXPRESS NNP 10_1101-2020_11_24_390039 147 80 35S 35S NNP 10_1101-2020_11_24_390039 147 81 Protein protein NN 10_1101-2020_11_24_390039 147 82 Labelling Labelling NNP 10_1101-2020_11_24_390039 147 83 Mix Mix NNP 10_1101-2020_11_24_390039 147 84 containing contain VBG 10_1101-2020_11_24_390039 147 85 [ [ -LRB- 10_1101-2020_11_24_390039 147 86 35S 35S NNP 10_1101-2020_11_24_390039 147 87 ] ] -RRB- 10_1101-2020_11_24_390039 147 88 methionine methionine NNP 10_1101-2020_11_24_390039 147 89 ( ( -LRB- 10_1101-2020_11_24_390039 147 90 Perkin Perkin NNP 10_1101-2020_11_24_390039 147 91 Elmer Elmer NNP 10_1101-2020_11_24_390039 147 92 ) ) -RRB- 10_1101-2020_11_24_390039 147 93 ( ( -LRB- 10_1101-2020_11_24_390039 147 94 0.533 0.533 CD 10_1101-2020_11_24_390039 147 95 MBq MBq NNS 10_1101-2020_11_24_390039 147 96 ; ; : 10_1101-2020_11_24_390039 147 97 30.15 30.15 CD 10_1101-2020_11_24_390039 147 98 TBq TBq NNP 10_1101-2020_11_24_390039 147 99 / / SYM 10_1101-2020_11_24_390039 147 100 mmol mmol NN 10_1101-2020_11_24_390039 147 101 ) ) -RRB- 10_1101-2020_11_24_390039 147 102 , , , 10_1101-2020_11_24_390039 147 103 25 25 CD 10_1101-2020_11_24_390039 147 104 μM μM NNP 10_1101-2020_11_24_390039 147 105 amino amino NNP 10_1101-2020_11_24_390039 147 106 acids acid NNS 10_1101-2020_11_24_390039 147 107 minus minus CC 10_1101-2020_11_24_390039 147 108 methionine methionine NNP 10_1101-2020_11_24_390039 147 109 ( ( -LRB- 10_1101-2020_11_24_390039 147 110 Promega Promega NNP 10_1101-2020_11_24_390039 147 111 ) ) -RRB- 10_1101-2020_11_24_390039 147 112 , , , 10_1101-2020_11_24_390039 147 113 1 1 CD 10_1101-2020_11_24_390039 147 114 µM µM NNP 10_1101-2020_11_24_390039 147 115 Ipom Ipom NNP 10_1101-2020_11_24_390039 147 116 - - HYPH 10_1101-2020_11_24_390039 147 117 F F NNP 10_1101-2020_11_24_390039 147 118 , , , 10_1101-2020_11_24_390039 147 119 or or CC 10_1101-2020_11_24_390039 147 120 an an DT 10_1101-2020_11_24_390039 147 121 equivalent equivalent JJ 10_1101-2020_11_24_390039 147 122 volume volume NN 10_1101-2020_11_24_390039 147 123 of of IN 10_1101-2020_11_24_390039 147 124 DMSO DMSO NNP 10_1101-2020_11_24_390039 147 125 , , , 10_1101-2020_11_24_390039 147 126 6.5 6.5 CD 10_1101-2020_11_24_390039 147 127 % % NN 10_1101-2020_11_24_390039 147 128 ( ( -LRB- 10_1101-2020_11_24_390039 147 129 v v NN 10_1101-2020_11_24_390039 147 130 / / SYM 10_1101-2020_11_24_390039 147 131 v v NN 10_1101-2020_11_24_390039 147 132 ) ) -RRB- 10_1101-2020_11_24_390039 147 133 ER- ER- NNP 10_1101-2020_11_24_390039 147 134 derived derive VBD 10_1101-2020_11_24_390039 147 135 microsomes microsome NNS 10_1101-2020_11_24_390039 147 136 or or CC 10_1101-2020_11_24_390039 147 137 SP SP NNP 10_1101-2020_11_24_390039 147 138 cells cell NNS 10_1101-2020_11_24_390039 147 139 and and CC 10_1101-2020_11_24_390039 147 140 ~10 ~10 . 10_1101-2020_11_24_390039 147 141 % % NN 10_1101-2020_11_24_390039 147 142 ( ( -LRB- 10_1101-2020_11_24_390039 147 143 v v NN 10_1101-2020_11_24_390039 147 144 / / SYM 10_1101-2020_11_24_390039 147 145 v v NN 10_1101-2020_11_24_390039 147 146 ) ) -RRB- 10_1101-2020_11_24_390039 147 147 of of XX 10_1101-2020_11_24_390039 147 148 in in FW 10_1101-2020_11_24_390039 147 149 vitro vitro FW 10_1101-2020_11_24_390039 147 150 transcribed transcribed NNP 10_1101-2020_11_24_390039 147 151 mRNA mRNA . 10_1101-2020_11_24_390039 147 152 ( ( -LRB- 10_1101-2020_11_24_390039 147 153 ~500 ~500 NNP 10_1101-2020_11_24_390039 147 154 ng ng NNP 10_1101-2020_11_24_390039 147 155 / / SYM 10_1101-2020_11_24_390039 147 156 μL μL NNP 10_1101-2020_11_24_390039 147 157 ) ) -RRB- 10_1101-2020_11_24_390039 147 158 encoding encode VBG 10_1101-2020_11_24_390039 147 159 the the DT 10_1101-2020_11_24_390039 147 160 relevant relevant JJ 10_1101-2020_11_24_390039 147 161 precursor precursor NN 10_1101-2020_11_24_390039 147 162 protein protein NN 10_1101-2020_11_24_390039 147 163 . . . 10_1101-2020_11_24_390039 148 1 Microsomal microsomal JJ 10_1101-2020_11_24_390039 148 2 translation translation NN 10_1101-2020_11_24_390039 148 3 reactions reaction NNS 10_1101-2020_11_24_390039 148 4 ( ( -LRB- 10_1101-2020_11_24_390039 148 5 20 20 CD 10_1101-2020_11_24_390039 148 6 μL μL NNP 10_1101-2020_11_24_390039 148 7 ) ) -RRB- 10_1101-2020_11_24_390039 148 8 were be VBD 10_1101-2020_11_24_390039 148 9 performed perform VBN 10_1101-2020_11_24_390039 148 10 for for IN 10_1101-2020_11_24_390039 148 11 30 30 CD 10_1101-2020_11_24_390039 148 12 min min NN 10_1101-2020_11_24_390039 148 13 at at IN 10_1101-2020_11_24_390039 148 14 30 30 CD 10_1101-2020_11_24_390039 148 15 ° ° , 10_1101-2020_11_24_390039 148 16 C C NNP 10_1101-2020_11_24_390039 148 17 whereas whereas IN 10_1101-2020_11_24_390039 148 18 those those DT 10_1101-2020_11_24_390039 148 19 using use VBG 10_1101-2020_11_24_390039 148 20 SP SP NNP 10_1101-2020_11_24_390039 148 21 HeLa HeLa NNP 10_1101-2020_11_24_390039 148 22 cells cell NNS 10_1101-2020_11_24_390039 148 23 were be VBD 10_1101-2020_11_24_390039 148 24 performed perform VBN 10_1101-2020_11_24_390039 148 25 on on IN 10_1101-2020_11_24_390039 148 26 a a DT 10_1101-2020_11_24_390039 148 27 1.5X 1.5x JJ 10_1101-2020_11_24_390039 148 28 scale scale NN 10_1101-2020_11_24_390039 148 29 ( ( -LRB- 10_1101-2020_11_24_390039 148 30 30 30 CD 10_1101-2020_11_24_390039 148 31 μL μL NNP 10_1101-2020_11_24_390039 148 32 translation translation NN 10_1101-2020_11_24_390039 148 33 reactions reaction NNS 10_1101-2020_11_24_390039 148 34 ) ) -RRB- 10_1101-2020_11_24_390039 148 35 for for IN 10_1101-2020_11_24_390039 148 36 1 1 CD 10_1101-2020_11_24_390039 148 37 h h NN 10_1101-2020_11_24_390039 148 38 at at IN 10_1101-2020_11_24_390039 148 39 30 30 CD 10_1101-2020_11_24_390039 148 40 ° ° , 10_1101-2020_11_24_390039 148 41 C C NNP 10_1101-2020_11_24_390039 148 42 . . . 10_1101-2020_11_24_390039 149 1 As as IN 10_1101-2020_11_24_390039 149 2 the the DT 10_1101-2020_11_24_390039 149 3 S S NNP 10_1101-2020_11_24_390039 149 4 protein protein NN 10_1101-2020_11_24_390039 149 5 was be VBD 10_1101-2020_11_24_390039 149 6 most most RBS 10_1101-2020_11_24_390039 149 7 efficiently efficiently RB 10_1101-2020_11_24_390039 149 8 synthesised synthesise VBN 10_1101-2020_11_24_390039 149 9 using use VBG 10_1101-2020_11_24_390039 149 10 the the DT 10_1101-2020_11_24_390039 149 11 TNT TNT NNP 10_1101-2020_11_24_390039 149 12 ® ® NNPS 10_1101-2020_11_24_390039 149 13 Coupled couple VBN 10_1101-2020_11_24_390039 149 14 system system NN 10_1101-2020_11_24_390039 149 15 ( ( -LRB- 10_1101-2020_11_24_390039 149 16 Fig fig NN 10_1101-2020_11_24_390039 149 17 . . . 10_1101-2020_11_24_390039 150 1 S1B S1B NNP 10_1101-2020_11_24_390039 150 2 ) ) -RRB- 10_1101-2020_11_24_390039 150 3 , , , 10_1101-2020_11_24_390039 150 4 import import NN 10_1101-2020_11_24_390039 150 5 assays assays RB 10_1101-2020_11_24_390039 150 6 of of IN 10_1101-2020_11_24_390039 150 7 the the DT 10_1101-2020_11_24_390039 150 8 comparatively comparatively RB 10_1101-2020_11_24_390039 150 9 higher high JJR 10_1101-2020_11_24_390039 150 10 molecular molecular JJ 10_1101-2020_11_24_390039 150 11 weight weight NN 10_1101-2020_11_24_390039 150 12 ACE2 ACE2 NNP 10_1101-2020_11_24_390039 150 13 and and CC 10_1101-2020_11_24_390039 150 14 S S NNP 10_1101-2020_11_24_390039 150 15 proteins protein NNS 10_1101-2020_11_24_390039 150 16 ( ( -LRB- 10_1101-2020_11_24_390039 150 17 50 50 CD 10_1101-2020_11_24_390039 150 18 μL μL NNP 10_1101-2020_11_24_390039 150 19 reactions reaction NNS 10_1101-2020_11_24_390039 150 20 ) ) -RRB- 10_1101-2020_11_24_390039 150 21 were be VBD 10_1101-2020_11_24_390039 150 22 both both DT 10_1101-2020_11_24_390039 150 23 performed perform VBN 10_1101-2020_11_24_390039 150 24 using use VBG 10_1101-2020_11_24_390039 150 25 the the DT 10_1101-2020_11_24_390039 150 26 TNT TNT NNP 10_1101-2020_11_24_390039 150 27 ® ® NNPS 10_1101-2020_11_24_390039 150 28 Coupled couple VBN 10_1101-2020_11_24_390039 150 29 Transcription/ transcription/ NN 10_1101-2020_11_24_390039 150 30 Translation translation NN 10_1101-2020_11_24_390039 150 31 system system NN 10_1101-2020_11_24_390039 150 32 ( ( -LRB- 10_1101-2020_11_24_390039 150 33 Promega Promega NNP 10_1101-2020_11_24_390039 150 34 ) ) -RRB- 10_1101-2020_11_24_390039 150 35 for for IN 10_1101-2020_11_24_390039 150 36 90 90 CD 10_1101-2020_11_24_390039 150 37 min min NN 10_1101-2020_11_24_390039 150 38 at at IN 10_1101-2020_11_24_390039 150 39 30 30 CD 10_1101-2020_11_24_390039 150 40 ° ° , 10_1101-2020_11_24_390039 150 41 C C NNP 10_1101-2020_11_24_390039 150 42 as as IN 10_1101-2020_11_24_390039 150 43 described describe VBN 10_1101-2020_11_24_390039 150 44 by by IN 10_1101-2020_11_24_390039 150 45 the the DT 10_1101-2020_11_24_390039 150 46 manufacturer manufacturer NN 10_1101-2020_11_24_390039 150 47 ( ( -LRB- 10_1101-2020_11_24_390039 150 48 ~50 ~50 NFP 10_1101-2020_11_24_390039 150 49 ng ng NN 10_1101-2020_11_24_390039 150 50 / / SYM 10_1101-2020_11_24_390039 150 51 μL μL NNP 10_1101-2020_11_24_390039 150 52 cDNA,1 cdna,1 XX 10_1101-2020_11_24_390039 150 53 µM µM NNP 10_1101-2020_11_24_390039 150 54 Ipom Ipom NNP 10_1101-2020_11_24_390039 150 55 - - HYPH 10_1101-2020_11_24_390039 150 56 F F NNP 10_1101-2020_11_24_390039 150 57 or or CC 10_1101-2020_11_24_390039 150 58 an an DT 10_1101-2020_11_24_390039 150 59 equivalent equivalent JJ 10_1101-2020_11_24_390039 150 60 .CC .CC NFP 10_1101-2020_11_24_390039 150 61 - - HYPH 10_1101-2020_11_24_390039 150 62 BY by IN 10_1101-2020_11_24_390039 150 63 - - HYPH 10_1101-2020_11_24_390039 150 64 ND ND NNP 10_1101-2020_11_24_390039 150 65 4.0 4.0 CD 10_1101-2020_11_24_390039 150 66 International international JJ 10_1101-2020_11_24_390039 150 67 licensemade licensemade NN 10_1101-2020_11_24_390039 150 68 available available JJ 10_1101-2020_11_24_390039 150 69 under under IN 10_1101-2020_11_24_390039 150 70 a a DT 10_1101-2020_11_24_390039 150 71 ( ( -LRB- 10_1101-2020_11_24_390039 150 72 which which WDT 10_1101-2020_11_24_390039 150 73 was be VBD 10_1101-2020_11_24_390039 150 74 not not RB 10_1101-2020_11_24_390039 150 75 certified certify VBN 10_1101-2020_11_24_390039 150 76 by by IN 10_1101-2020_11_24_390039 150 77 peer peer NN 10_1101-2020_11_24_390039 150 78 review review NN 10_1101-2020_11_24_390039 150 79 ) ) -RRB- 10_1101-2020_11_24_390039 150 80 is be VBZ 10_1101-2020_11_24_390039 150 81 the the DT 10_1101-2020_11_24_390039 150 82 author author NN 10_1101-2020_11_24_390039 150 83 / / SYM 10_1101-2020_11_24_390039 150 84 funder funder NN 10_1101-2020_11_24_390039 150 85 , , , 10_1101-2020_11_24_390039 150 86 who who WP 10_1101-2020_11_24_390039 150 87 has have VBZ 10_1101-2020_11_24_390039 150 88 granted grant VBN 10_1101-2020_11_24_390039 150 89 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 150 90 a a DT 10_1101-2020_11_24_390039 150 91 license license NN 10_1101-2020_11_24_390039 150 92 to to TO 10_1101-2020_11_24_390039 150 93 display display VB 10_1101-2020_11_24_390039 150 94 the the DT 10_1101-2020_11_24_390039 150 95 preprint preprint NN 10_1101-2020_11_24_390039 150 96 in in IN 10_1101-2020_11_24_390039 150 97 perpetuity perpetuity NN 10_1101-2020_11_24_390039 150 98 . . . 10_1101-2020_11_24_390039 151 1 It -PRON- PRP 10_1101-2020_11_24_390039 151 2 is be VBZ 10_1101-2020_11_24_390039 151 3 The the DT 10_1101-2020_11_24_390039 151 4 copyright copyright NN 10_1101-2020_11_24_390039 151 5 holder holder NN 10_1101-2020_11_24_390039 151 6 for for IN 10_1101-2020_11_24_390039 151 7 this this DT 10_1101-2020_11_24_390039 151 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 151 9 version version NN 10_1101-2020_11_24_390039 151 10 posted post VBD 10_1101-2020_11_24_390039 151 11 January January NNP 10_1101-2020_11_24_390039 151 12 5 5 CD 10_1101-2020_11_24_390039 151 13 , , , 10_1101-2020_11_24_390039 151 14 2021 2021 CD 10_1101-2020_11_24_390039 151 15 . . . 10_1101-2020_11_24_390039 151 16 ; ; : 10_1101-2020_11_24_390039 151 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 151 18 : : : 10_1101-2020_11_24_390039 151 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 151 20 preprint preprint JJ 10_1101-2020_11_24_390039 151 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 151 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 151 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 151 24 - - HYPH 10_1101-2020_11_24_390039 151 25 F F NNP 10_1101-2020_11_24_390039 151 26 as as IN 10_1101-2020_11_24_390039 151 27 a a DT 10_1101-2020_11_24_390039 151 28 potential potential JJ 10_1101-2020_11_24_390039 151 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 151 30 agent agent NN 10_1101-2020_11_24_390039 151 31 Page Page NNP 10_1101-2020_11_24_390039 151 32 12 12 CD 10_1101-2020_11_24_390039 151 33 of of IN 10_1101-2020_11_24_390039 151 34 21 21 CD 10_1101-2020_11_24_390039 151 35 volume volume NN 10_1101-2020_11_24_390039 151 36 of of IN 10_1101-2020_11_24_390039 151 37 DMSO DMSO NNP 10_1101-2020_11_24_390039 151 38 , , , 10_1101-2020_11_24_390039 151 39 12 12 CD 10_1101-2020_11_24_390039 151 40 % % NN 10_1101-2020_11_24_390039 151 41 ( ( -LRB- 10_1101-2020_11_24_390039 151 42 v v NN 10_1101-2020_11_24_390039 151 43 / / SYM 10_1101-2020_11_24_390039 151 44 v v NN 10_1101-2020_11_24_390039 151 45 ) ) -RRB- 10_1101-2020_11_24_390039 151 46 ER ER NNP 10_1101-2020_11_24_390039 151 47 - - HYPH 10_1101-2020_11_24_390039 151 48 derived derive VBN 10_1101-2020_11_24_390039 151 49 microsomes microsome NNS 10_1101-2020_11_24_390039 151 50 or or CC 10_1101-2020_11_24_390039 151 51 SP SP NNP 10_1101-2020_11_24_390039 151 52 cells cell NNS 10_1101-2020_11_24_390039 151 53 ) ) -RRB- 10_1101-2020_11_24_390039 151 54 . . . 10_1101-2020_11_24_390039 152 1 All all DT 10_1101-2020_11_24_390039 152 2 translation translation NN 10_1101-2020_11_24_390039 152 3 reactions reaction NNS 10_1101-2020_11_24_390039 152 4 were be VBD 10_1101-2020_11_24_390039 152 5 finished finish VBN 10_1101-2020_11_24_390039 152 6 by by IN 10_1101-2020_11_24_390039 152 7 incubating incubate VBG 10_1101-2020_11_24_390039 152 8 with with IN 10_1101-2020_11_24_390039 152 9 0.1 0.1 CD 10_1101-2020_11_24_390039 152 10 mM mm NN 10_1101-2020_11_24_390039 152 11 puromycin puromycin JJ 10_1101-2020_11_24_390039 152 12 for for IN 10_1101-2020_11_24_390039 152 13 10 10 CD 10_1101-2020_11_24_390039 152 14 min min NN 10_1101-2020_11_24_390039 152 15 at at IN 10_1101-2020_11_24_390039 152 16 30 30 CD 10_1101-2020_11_24_390039 152 17 ° ° , 10_1101-2020_11_24_390039 152 18 C C NNP 10_1101-2020_11_24_390039 152 19 to to TO 10_1101-2020_11_24_390039 152 20 ensure ensure VB 10_1101-2020_11_24_390039 152 21 translation translation NN 10_1101-2020_11_24_390039 152 22 termination termination NN 10_1101-2020_11_24_390039 152 23 and and CC 10_1101-2020_11_24_390039 152 24 ribosome ribosome NN 10_1101-2020_11_24_390039 152 25 release release NN 10_1101-2020_11_24_390039 152 26 of of IN 10_1101-2020_11_24_390039 152 27 newly newly RB 10_1101-2020_11_24_390039 152 28 synthesised synthesise VBN 10_1101-2020_11_24_390039 152 29 proteins protein NNS 10_1101-2020_11_24_390039 152 30 prior prior RB 10_1101-2020_11_24_390039 152 31 to to IN 10_1101-2020_11_24_390039 152 32 analysis analysis NN 10_1101-2020_11_24_390039 152 33 . . . 10_1101-2020_11_24_390039 153 1 Recovery recovery NN 10_1101-2020_11_24_390039 153 2 and and CC 10_1101-2020_11_24_390039 153 3 analysis analysis NN 10_1101-2020_11_24_390039 153 4 of of IN 10_1101-2020_11_24_390039 153 5 radiolabelled radiolabelle VBN 10_1101-2020_11_24_390039 153 6 products product NNS 10_1101-2020_11_24_390039 153 7 Following follow VBG 10_1101-2020_11_24_390039 153 8 puromycin puromycin JJ 10_1101-2020_11_24_390039 153 9 treatment treatment NN 10_1101-2020_11_24_390039 153 10 , , , 10_1101-2020_11_24_390039 153 11 microsomal microsomal JJ 10_1101-2020_11_24_390039 153 12 membrane membrane NN 10_1101-2020_11_24_390039 153 13 - - HYPH 10_1101-2020_11_24_390039 153 14 associated associate VBN 10_1101-2020_11_24_390039 153 15 fractions fraction NNS 10_1101-2020_11_24_390039 153 16 were be VBD 10_1101-2020_11_24_390039 153 17 recovered recover VBN 10_1101-2020_11_24_390039 153 18 by by IN 10_1101-2020_11_24_390039 153 19 centrifugation centrifugation NN 10_1101-2020_11_24_390039 153 20 through through IN 10_1101-2020_11_24_390039 153 21 an an DT 10_1101-2020_11_24_390039 153 22 80 80 CD 10_1101-2020_11_24_390039 153 23 μL μl NN 10_1101-2020_11_24_390039 153 24 high high JJ 10_1101-2020_11_24_390039 153 25 - - HYPH 10_1101-2020_11_24_390039 153 26 salt salt NN 10_1101-2020_11_24_390039 153 27 cushion cushion NN 10_1101-2020_11_24_390039 153 28 ( ( -LRB- 10_1101-2020_11_24_390039 153 29 0.75 0.75 CD 10_1101-2020_11_24_390039 153 30 M m NN 10_1101-2020_11_24_390039 153 31 sucrose sucrose NN 10_1101-2020_11_24_390039 153 32 , , , 10_1101-2020_11_24_390039 153 33 0.5 0.5 CD 10_1101-2020_11_24_390039 153 34 M M NNP 10_1101-2020_11_24_390039 153 35 KOAc KOAc NNS 10_1101-2020_11_24_390039 153 36 , , , 10_1101-2020_11_24_390039 153 37 5 5 CD 10_1101-2020_11_24_390039 153 38 mM mm NN 10_1101-2020_11_24_390039 153 39 Mg(OAc)2 mg(oac)2 NN 10_1101-2020_11_24_390039 153 40 , , , 10_1101-2020_11_24_390039 153 41 50 50 CD 10_1101-2020_11_24_390039 153 42 mM mM NNP 10_1101-2020_11_24_390039 153 43 Hepes Hepes NNP 10_1101-2020_11_24_390039 153 44 - - HYPH 10_1101-2020_11_24_390039 153 45 KOH KOH NNP 10_1101-2020_11_24_390039 153 46 , , , 10_1101-2020_11_24_390039 153 47 pH pH NNP 10_1101-2020_11_24_390039 153 48 7.9 7.9 CD 10_1101-2020_11_24_390039 153 49 ) ) -RRB- 10_1101-2020_11_24_390039 153 50 at at IN 10_1101-2020_11_24_390039 153 51 100,000 100,000 CD 10_1101-2020_11_24_390039 153 52 g g NN 10_1101-2020_11_24_390039 153 53 for for IN 10_1101-2020_11_24_390039 153 54 10 10 CD 10_1101-2020_11_24_390039 153 55 min min NN 10_1101-2020_11_24_390039 153 56 at at IN 10_1101-2020_11_24_390039 153 57 4 4 CD 10_1101-2020_11_24_390039 153 58 ° ° NNS 10_1101-2020_11_24_390039 153 59 C C NNP 10_1101-2020_11_24_390039 153 60 and and CC 10_1101-2020_11_24_390039 153 61 the the DT 10_1101-2020_11_24_390039 153 62 pellet pellet NN 10_1101-2020_11_24_390039 153 63 suspended suspend VBN 10_1101-2020_11_24_390039 153 64 directly directly RB 10_1101-2020_11_24_390039 153 65 in in IN 10_1101-2020_11_24_390039 153 66 SDS SDS NNP 10_1101-2020_11_24_390039 153 67 sample sample NN 10_1101-2020_11_24_390039 153 68 buffer buffer NN 10_1101-2020_11_24_390039 153 69 . . . 10_1101-2020_11_24_390039 154 1 To to TO 10_1101-2020_11_24_390039 154 2 confirm confirm VB 10_1101-2020_11_24_390039 154 3 the the DT 10_1101-2020_11_24_390039 154 4 topology topology NN 10_1101-2020_11_24_390039 154 5 of of IN 10_1101-2020_11_24_390039 154 6 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 154 7 ( ( -LRB- 10_1101-2020_11_24_390039 154 8 Fig Fig NNP 10_1101-2020_11_24_390039 154 9 . . . 10_1101-2020_11_24_390039 155 1 S2 s2 NN 10_1101-2020_11_24_390039 155 2 ) ) -RRB- 10_1101-2020_11_24_390039 155 3 , , , 10_1101-2020_11_24_390039 155 4 the the DT 10_1101-2020_11_24_390039 155 5 membrane membrane NN 10_1101-2020_11_24_390039 155 6 - - HYPH 10_1101-2020_11_24_390039 155 7 associated associate VBN 10_1101-2020_11_24_390039 155 8 fraction fraction NN 10_1101-2020_11_24_390039 155 9 of of IN 10_1101-2020_11_24_390039 155 10 the the DT 10_1101-2020_11_24_390039 155 11 doubly doubly RB 10_1101-2020_11_24_390039 155 12 - - HYPH 10_1101-2020_11_24_390039 155 13 OPG2-tagged opg2-tagged JJ 10_1101-2020_11_24_390039 155 14 form form NN 10_1101-2020_11_24_390039 155 15 ( ( -LRB- 10_1101-2020_11_24_390039 155 16 OPG2-ORF6-OPG2 OPG2-ORF6-OPG2 NNP 10_1101-2020_11_24_390039 155 17 ) ) -RRB- 10_1101-2020_11_24_390039 155 18 was be VBD 10_1101-2020_11_24_390039 155 19 resuspended resuspend VBN 10_1101-2020_11_24_390039 155 20 in in IN 10_1101-2020_11_24_390039 155 21 KHM KHM NNP 10_1101-2020_11_24_390039 155 22 buffer buffer NN 10_1101-2020_11_24_390039 155 23 ( ( -LRB- 10_1101-2020_11_24_390039 155 24 20 20 CD 10_1101-2020_11_24_390039 155 25 μL μL NNP 10_1101-2020_11_24_390039 155 26 ) ) -RRB- 10_1101-2020_11_24_390039 155 27 and and CC 10_1101-2020_11_24_390039 155 28 subjected subject VBN 10_1101-2020_11_24_390039 155 29 to to IN 10_1101-2020_11_24_390039 155 30 either either CC 10_1101-2020_11_24_390039 155 31 carbonate carbonate NN 10_1101-2020_11_24_390039 155 32 extraction extraction NN 10_1101-2020_11_24_390039 155 33 ( ( -LRB- 10_1101-2020_11_24_390039 155 34 0.1 0.1 CD 10_1101-2020_11_24_390039 155 35 M M NNP 10_1101-2020_11_24_390039 155 36 Na2CO3 Na2CO3 NNP 10_1101-2020_11_24_390039 155 37 , , , 10_1101-2020_11_24_390039 155 38 pH pH NNP 10_1101-2020_11_24_390039 155 39 11.3 11.3 CD 10_1101-2020_11_24_390039 155 40 ) ) -RRB- 10_1101-2020_11_24_390039 155 41 ( ( -LRB- 10_1101-2020_11_24_390039 155 42 McKenna McKenna NNP 10_1101-2020_11_24_390039 155 43 et et NNP 10_1101-2020_11_24_390039 155 44 al al NNP 10_1101-2020_11_24_390039 155 45 . . NNP 10_1101-2020_11_24_390039 155 46 , , , 10_1101-2020_11_24_390039 155 47 2016 2016 CD 10_1101-2020_11_24_390039 155 48 ) ) -RRB- 10_1101-2020_11_24_390039 155 49 or or CC 10_1101-2020_11_24_390039 155 50 a a DT 10_1101-2020_11_24_390039 155 51 protease protease NN 10_1101-2020_11_24_390039 155 52 protection protection NN 10_1101-2020_11_24_390039 155 53 assay assay NN 10_1101-2020_11_24_390039 155 54 using use VBG 10_1101-2020_11_24_390039 155 55 trypsin trypsin NNP 10_1101-2020_11_24_390039 155 56 ( ( -LRB- 10_1101-2020_11_24_390039 155 57 1 1 CD 10_1101-2020_11_24_390039 155 58 μg μg NNP 10_1101-2020_11_24_390039 155 59 / / SYM 10_1101-2020_11_24_390039 155 60 mL ml NN 10_1101-2020_11_24_390039 155 61 ) ) -RRB- 10_1101-2020_11_24_390039 155 62 with with IN 10_1101-2020_11_24_390039 155 63 or or CC 10_1101-2020_11_24_390039 155 64 without without IN 10_1101-2020_11_24_390039 155 65 0.1 0.1 CD 10_1101-2020_11_24_390039 155 66 % % NN 10_1101-2020_11_24_390039 155 67 Triton Triton NNP 10_1101-2020_11_24_390039 155 68 X-100 X-100 NNP 10_1101-2020_11_24_390039 155 69 ( ( -LRB- 10_1101-2020_11_24_390039 155 70 Ray Ray NNP 10_1101-2020_11_24_390039 155 71 - - HYPH 10_1101-2020_11_24_390039 155 72 Sinha Sinha NNP 10_1101-2020_11_24_390039 155 73 et et NNP 10_1101-2020_11_24_390039 155 74 al al NNP 10_1101-2020_11_24_390039 155 75 . . NNP 10_1101-2020_11_24_390039 155 76 , , , 10_1101-2020_11_24_390039 155 77 2009 2009 CD 10_1101-2020_11_24_390039 155 78 ) ) -RRB- 10_1101-2020_11_24_390039 155 79 prior prior RB 10_1101-2020_11_24_390039 155 80 to to IN 10_1101-2020_11_24_390039 155 81 suspension suspension NN 10_1101-2020_11_24_390039 155 82 in in IN 10_1101-2020_11_24_390039 155 83 SDS SDS NNP 10_1101-2020_11_24_390039 155 84 sample sample NN 10_1101-2020_11_24_390039 155 85 buffer buffer NN 10_1101-2020_11_24_390039 155 86 . . . 10_1101-2020_11_24_390039 156 1 For for IN 10_1101-2020_11_24_390039 156 2 translation translation NN 10_1101-2020_11_24_390039 156 3 reactions reaction NNS 10_1101-2020_11_24_390039 156 4 using use VBG 10_1101-2020_11_24_390039 156 5 SP SP NNP 10_1101-2020_11_24_390039 156 6 cells cell NNS 10_1101-2020_11_24_390039 156 7 , , , 10_1101-2020_11_24_390039 156 8 the the DT 10_1101-2020_11_24_390039 156 9 total total JJ 10_1101-2020_11_24_390039 156 10 reaction reaction NN 10_1101-2020_11_24_390039 156 11 material material NN 10_1101-2020_11_24_390039 156 12 was be VBD 10_1101-2020_11_24_390039 156 13 diluted dilute VBN 10_1101-2020_11_24_390039 156 14 with with IN 10_1101-2020_11_24_390039 156 15 nine nine CD 10_1101-2020_11_24_390039 156 16 volumes volume NNS 10_1101-2020_11_24_390039 156 17 of of IN 10_1101-2020_11_24_390039 156 18 Triton Triton NNP 10_1101-2020_11_24_390039 156 19 immunoprecipitation immunoprecipitation NN 10_1101-2020_11_24_390039 156 20 buffer buffer NN 10_1101-2020_11_24_390039 156 21 ( ( -LRB- 10_1101-2020_11_24_390039 156 22 10 10 CD 10_1101-2020_11_24_390039 156 23 mM mM NNP 10_1101-2020_11_24_390039 156 24 Tris Tris NNP 10_1101-2020_11_24_390039 156 25 - - HYPH 10_1101-2020_11_24_390039 156 26 HCl HCl NNP 10_1101-2020_11_24_390039 156 27 , , , 10_1101-2020_11_24_390039 156 28 140 140 CD 10_1101-2020_11_24_390039 156 29 mM mM NNP 10_1101-2020_11_24_390039 156 30 NaCl NaCl NNP 10_1101-2020_11_24_390039 156 31 , , , 10_1101-2020_11_24_390039 156 32 1 1 CD 10_1101-2020_11_24_390039 156 33 mM mm NN 10_1101-2020_11_24_390039 156 34 EDTA EDTA NNP 10_1101-2020_11_24_390039 156 35 , , , 10_1101-2020_11_24_390039 156 36 1 1 CD 10_1101-2020_11_24_390039 156 37 % % NN 10_1101-2020_11_24_390039 156 38 Triton Triton NNP 10_1101-2020_11_24_390039 156 39 X-100 X-100 NNP 10_1101-2020_11_24_390039 156 40 , , , 10_1101-2020_11_24_390039 156 41 5 5 CD 10_1101-2020_11_24_390039 156 42 mM mm NN 10_1101-2020_11_24_390039 156 43 PMSF pmsf NN 10_1101-2020_11_24_390039 156 44 , , , 10_1101-2020_11_24_390039 156 45 1 1 CD 10_1101-2020_11_24_390039 156 46 mM mm NN 10_1101-2020_11_24_390039 156 47 methionine methionine NN 10_1101-2020_11_24_390039 156 48 ( ( -LRB- 10_1101-2020_11_24_390039 156 49 to to TO 10_1101-2020_11_24_390039 156 50 prevent prevent VB 10_1101-2020_11_24_390039 156 51 background background NN 10_1101-2020_11_24_390039 156 52 from from IN 10_1101-2020_11_24_390039 156 53 the the DT 10_1101-2020_11_24_390039 156 54 radiolabelled radiolabelle VBN 10_1101-2020_11_24_390039 156 55 methionine methionine NN 10_1101-2020_11_24_390039 156 56 ) ) -RRB- 10_1101-2020_11_24_390039 156 57 , , , 10_1101-2020_11_24_390039 156 58 pH pH NNP 10_1101-2020_11_24_390039 156 59 7.5 7.5 CD 10_1101-2020_11_24_390039 156 60 ) ) -RRB- 10_1101-2020_11_24_390039 156 61 . . . 10_1101-2020_11_24_390039 157 1 Samples sample NNS 10_1101-2020_11_24_390039 157 2 were be VBD 10_1101-2020_11_24_390039 157 3 incubated incubate VBN 10_1101-2020_11_24_390039 157 4 under under IN 10_1101-2020_11_24_390039 157 5 constant constant JJ 10_1101-2020_11_24_390039 157 6 agitation agitation NN 10_1101-2020_11_24_390039 157 7 with with IN 10_1101-2020_11_24_390039 157 8 an an DT 10_1101-2020_11_24_390039 157 9 antibody antibody NN 10_1101-2020_11_24_390039 157 10 recognising recognise VBG 10_1101-2020_11_24_390039 157 11 the the DT 10_1101-2020_11_24_390039 157 12 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 157 13 epitope epitope NN 10_1101-2020_11_24_390039 157 14 ( ( -LRB- 10_1101-2020_11_24_390039 157 15 1:200 1:200 CD 10_1101-2020_11_24_390039 157 16 dilution dilution NN 10_1101-2020_11_24_390039 157 17 ) ) -RRB- 10_1101-2020_11_24_390039 157 18 for for IN 10_1101-2020_11_24_390039 157 19 16 16 CD 10_1101-2020_11_24_390039 157 20 h h NN 10_1101-2020_11_24_390039 157 21 at at IN 10_1101-2020_11_24_390039 157 22 4 4 CD 10_1101-2020_11_24_390039 157 23 ° ° , 10_1101-2020_11_24_390039 157 24 C C NNP 10_1101-2020_11_24_390039 157 25 to to TO 10_1101-2020_11_24_390039 157 26 recover recover VB 10_1101-2020_11_24_390039 157 27 both both DT 10_1101-2020_11_24_390039 157 28 the the DT 10_1101-2020_11_24_390039 157 29 membrane membrane NN 10_1101-2020_11_24_390039 157 30 - - HYPH 10_1101-2020_11_24_390039 157 31 associated associate VBN 10_1101-2020_11_24_390039 157 32 and and CC 10_1101-2020_11_24_390039 157 33 non non JJ 10_1101-2020_11_24_390039 157 34 - - JJ 10_1101-2020_11_24_390039 157 35 targeted target VBN 10_1101-2020_11_24_390039 157 36 nascent nascent JJ 10_1101-2020_11_24_390039 157 37 chains chain NNS 10_1101-2020_11_24_390039 157 38 . . . 10_1101-2020_11_24_390039 158 1 Samples sample NNS 10_1101-2020_11_24_390039 158 2 were be VBD 10_1101-2020_11_24_390039 158 3 next next RB 10_1101-2020_11_24_390039 158 4 incubated incubate VBN 10_1101-2020_11_24_390039 158 5 under under IN 10_1101-2020_11_24_390039 158 6 constant constant JJ 10_1101-2020_11_24_390039 158 7 agitation agitation NN 10_1101-2020_11_24_390039 158 8 with with IN 10_1101-2020_11_24_390039 158 9 10 10 CD 10_1101-2020_11_24_390039 158 10 % % NN 10_1101-2020_11_24_390039 158 11 ( ( -LRB- 10_1101-2020_11_24_390039 158 12 v v NN 10_1101-2020_11_24_390039 158 13 / / SYM 10_1101-2020_11_24_390039 158 14 v v NN 10_1101-2020_11_24_390039 158 15 ) ) -RRB- 10_1101-2020_11_24_390039 158 16 Protein Protein NNP 10_1101-2020_11_24_390039 158 17 - - HYPH 10_1101-2020_11_24_390039 158 18 A a NN 10_1101-2020_11_24_390039 158 19 - - HYPH 10_1101-2020_11_24_390039 158 20 Sepharose sepharose NN 10_1101-2020_11_24_390039 158 21 beads bead NNS 10_1101-2020_11_24_390039 158 22 ( ( -LRB- 10_1101-2020_11_24_390039 158 23 Genscript genscript NN 10_1101-2020_11_24_390039 158 24 ) ) -RRB- 10_1101-2020_11_24_390039 158 25 for for IN 10_1101-2020_11_24_390039 158 26 a a DT 10_1101-2020_11_24_390039 158 27 further further JJ 10_1101-2020_11_24_390039 158 28 2 2 CD 10_1101-2020_11_24_390039 158 29 h h NN 10_1101-2020_11_24_390039 158 30 at at IN 10_1101-2020_11_24_390039 158 31 4 4 CD 10_1101-2020_11_24_390039 158 32 ° ° NNS 10_1101-2020_11_24_390039 158 33 C C NNP 10_1101-2020_11_24_390039 158 34 before before IN 10_1101-2020_11_24_390039 158 35 recovery recovery NN 10_1101-2020_11_24_390039 158 36 by by IN 10_1101-2020_11_24_390039 158 37 centrifugation centrifugation NN 10_1101-2020_11_24_390039 158 38 at at IN 10_1101-2020_11_24_390039 158 39 13,000 13,000 CD 10_1101-2020_11_24_390039 158 40 g g NN 10_1101-2020_11_24_390039 158 41 for for IN 10_1101-2020_11_24_390039 158 42 1 1 CD 10_1101-2020_11_24_390039 158 43 min min NN 10_1101-2020_11_24_390039 158 44 . . . 10_1101-2020_11_24_390039 159 1 Protein protein NN 10_1101-2020_11_24_390039 159 2 - - HYPH 10_1101-2020_11_24_390039 159 3 A a NN 10_1101-2020_11_24_390039 159 4 - - HYPH 10_1101-2020_11_24_390039 159 5 Sepharose sepharose NN 10_1101-2020_11_24_390039 159 6 beads bead NNS 10_1101-2020_11_24_390039 159 7 were be VBD 10_1101-2020_11_24_390039 159 8 washed wash VBN 10_1101-2020_11_24_390039 159 9 twice twice RB 10_1101-2020_11_24_390039 159 10 with with IN 10_1101-2020_11_24_390039 159 11 Triton Triton NNP 10_1101-2020_11_24_390039 159 12 immunoprecipitation immunoprecipitation NN 10_1101-2020_11_24_390039 159 13 buffer buffer NN 10_1101-2020_11_24_390039 159 14 prior prior RB 10_1101-2020_11_24_390039 159 15 to to IN 10_1101-2020_11_24_390039 159 16 suspension suspension NN 10_1101-2020_11_24_390039 159 17 in in IN 10_1101-2020_11_24_390039 159 18 SDS SDS NNP 10_1101-2020_11_24_390039 159 19 sample sample NN 10_1101-2020_11_24_390039 159 20 buffer buffer NN 10_1101-2020_11_24_390039 159 21 . . . 10_1101-2020_11_24_390039 160 1 Where where WRB 10_1101-2020_11_24_390039 160 2 indicated indicate VBN 10_1101-2020_11_24_390039 160 3 , , , 10_1101-2020_11_24_390039 160 4 samples sample NNS 10_1101-2020_11_24_390039 160 5 were be VBD 10_1101-2020_11_24_390039 160 6 treated treat VBN 10_1101-2020_11_24_390039 160 7 with with IN 10_1101-2020_11_24_390039 160 8 1000 1000 CD 10_1101-2020_11_24_390039 160 9 U u NN 10_1101-2020_11_24_390039 160 10 of of IN 10_1101-2020_11_24_390039 160 11 a a DT 10_1101-2020_11_24_390039 160 12 form form NN 10_1101-2020_11_24_390039 160 13 of of IN 10_1101-2020_11_24_390039 160 14 Endoglycosidase Endoglycosidase NNP 10_1101-2020_11_24_390039 160 15 H H NNP 10_1101-2020_11_24_390039 160 16 that that WDT 10_1101-2020_11_24_390039 160 17 does do VBZ 10_1101-2020_11_24_390039 160 18 not not RB 10_1101-2020_11_24_390039 160 19 co co VB 10_1101-2020_11_24_390039 160 20 - - VB 10_1101-2020_11_24_390039 160 21 migrate migrate VB 10_1101-2020_11_24_390039 160 22 with with IN 10_1101-2020_11_24_390039 160 23 and and CC 10_1101-2020_11_24_390039 160 24 hence hence RB 10_1101-2020_11_24_390039 160 25 potentially potentially RB 10_1101-2020_11_24_390039 160 26 distort distort VBP 10_1101-2020_11_24_390039 160 27 the the DT 10_1101-2020_11_24_390039 160 28 radiolabelled radiolabelle VBN 10_1101-2020_11_24_390039 160 29 products product NNS 10_1101-2020_11_24_390039 160 30 when when WRB 10_1101-2020_11_24_390039 160 31 resolved resolve VBN 10_1101-2020_11_24_390039 160 32 : : : 10_1101-2020_11_24_390039 160 33 Endoglycosidase Endoglycosidase NNP 10_1101-2020_11_24_390039 160 34 Hf Hf NNP 10_1101-2020_11_24_390039 160 35 ( ( -LRB- 10_1101-2020_11_24_390039 160 36 translation translation NN 10_1101-2020_11_24_390039 160 37 products product NNS 10_1101-2020_11_24_390039 160 38 of of IN 10_1101-2020_11_24_390039 160 39 ~10 ~10 NFP 10_1101-2020_11_24_390039 160 40 - - : 10_1101-2020_11_24_390039 160 41 50 50 CD 10_1101-2020_11_24_390039 160 42 kDa kDa NNS 10_1101-2020_11_24_390039 160 43 ; ; : 10_1101-2020_11_24_390039 160 44 New New NNP 10_1101-2020_11_24_390039 160 45 England England NNP 10_1101-2020_11_24_390039 160 46 Biolabs Biolabs NNP 10_1101-2020_11_24_390039 160 47 , , , 10_1101-2020_11_24_390039 160 48 P0703S P0703S NNPS 10_1101-2020_11_24_390039 160 49 ) ) -RRB- 10_1101-2020_11_24_390039 160 50 or or CC 10_1101-2020_11_24_390039 160 51 Endoglycosidase Endoglycosidase NNP 10_1101-2020_11_24_390039 160 52 H H NNP 10_1101-2020_11_24_390039 160 53 ( ( -LRB- 10_1101-2020_11_24_390039 160 54 translation translation NN 10_1101-2020_11_24_390039 160 55 products product NNS 10_1101-2020_11_24_390039 160 56 of of IN 10_1101-2020_11_24_390039 160 57 ~50 ~50 NFP 10_1101-2020_11_24_390039 160 58 - - HYPH 10_1101-2020_11_24_390039 160 59 150 150 CD 10_1101-2020_11_24_390039 160 60 kDa kda NN 10_1101-2020_11_24_390039 160 61 protein protein NN 10_1101-2020_11_24_390039 160 62 substrates substrate NNS 10_1101-2020_11_24_390039 160 63 ; ; : 10_1101-2020_11_24_390039 160 64 New New NNP 10_1101-2020_11_24_390039 160 65 England England NNP 10_1101-2020_11_24_390039 160 66 Biolabs Biolabs NNP 10_1101-2020_11_24_390039 160 67 , , , 10_1101-2020_11_24_390039 160 68 P0702S P0702S NNP 10_1101-2020_11_24_390039 160 69 ) ) -RRB- 10_1101-2020_11_24_390039 160 70 . . . 10_1101-2020_11_24_390039 161 1 All all DT 10_1101-2020_11_24_390039 161 2 samples sample NNS 10_1101-2020_11_24_390039 161 3 were be VBD 10_1101-2020_11_24_390039 161 4 solubilised solubilise VBN 10_1101-2020_11_24_390039 161 5 for for IN 10_1101-2020_11_24_390039 161 6 12 12 CD 10_1101-2020_11_24_390039 161 7 h h NN 10_1101-2020_11_24_390039 161 8 at at IN 10_1101-2020_11_24_390039 161 9 37 37 CD 10_1101-2020_11_24_390039 161 10 ° ° NNS 10_1101-2020_11_24_390039 161 11 C C NNP 10_1101-2020_11_24_390039 161 12 and and CC 10_1101-2020_11_24_390039 161 13 then then RB 10_1101-2020_11_24_390039 161 14 sonicated sonicate VBD 10_1101-2020_11_24_390039 161 15 prior prior RB 10_1101-2020_11_24_390039 161 16 to to IN 10_1101-2020_11_24_390039 161 17 resolution resolution NN 10_1101-2020_11_24_390039 161 18 by by IN 10_1101-2020_11_24_390039 161 19 SDS SDS NNP 10_1101-2020_11_24_390039 161 20 - - HYPH 10_1101-2020_11_24_390039 161 21 PAGE PAGE NNP 10_1101-2020_11_24_390039 161 22 ( ( -LRB- 10_1101-2020_11_24_390039 161 23 10 10 CD 10_1101-2020_11_24_390039 161 24 % % NN 10_1101-2020_11_24_390039 161 25 or or CC 10_1101-2020_11_24_390039 161 26 16 16 CD 10_1101-2020_11_24_390039 161 27 % % NN 10_1101-2020_11_24_390039 161 28 PAGE PAGE NNP 10_1101-2020_11_24_390039 161 29 , , , 10_1101-2020_11_24_390039 161 30 120V 120v CD 10_1101-2020_11_24_390039 161 31 , , , 10_1101-2020_11_24_390039 161 32 120 120 CD 10_1101-2020_11_24_390039 161 33 - - SYM 10_1101-2020_11_24_390039 161 34 180 180 CD 10_1101-2020_11_24_390039 161 35 min min NN 10_1101-2020_11_24_390039 161 36 ) ) -RRB- 10_1101-2020_11_24_390039 161 37 . . . 10_1101-2020_11_24_390039 162 1 Gels gel NNS 10_1101-2020_11_24_390039 162 2 were be VBD 10_1101-2020_11_24_390039 162 3 .CC .CC : 10_1101-2020_11_24_390039 162 4 - - HYPH 10_1101-2020_11_24_390039 162 5 BY by IN 10_1101-2020_11_24_390039 162 6 - - HYPH 10_1101-2020_11_24_390039 162 7 ND ND NNP 10_1101-2020_11_24_390039 162 8 4.0 4.0 CD 10_1101-2020_11_24_390039 162 9 International international JJ 10_1101-2020_11_24_390039 162 10 licensemade licensemade NN 10_1101-2020_11_24_390039 162 11 available available JJ 10_1101-2020_11_24_390039 162 12 under under IN 10_1101-2020_11_24_390039 162 13 a a DT 10_1101-2020_11_24_390039 162 14 ( ( -LRB- 10_1101-2020_11_24_390039 162 15 which which WDT 10_1101-2020_11_24_390039 162 16 was be VBD 10_1101-2020_11_24_390039 162 17 not not RB 10_1101-2020_11_24_390039 162 18 certified certify VBN 10_1101-2020_11_24_390039 162 19 by by IN 10_1101-2020_11_24_390039 162 20 peer peer NN 10_1101-2020_11_24_390039 162 21 review review NN 10_1101-2020_11_24_390039 162 22 ) ) -RRB- 10_1101-2020_11_24_390039 162 23 is be VBZ 10_1101-2020_11_24_390039 162 24 the the DT 10_1101-2020_11_24_390039 162 25 author author NN 10_1101-2020_11_24_390039 162 26 / / SYM 10_1101-2020_11_24_390039 162 27 funder funder NN 10_1101-2020_11_24_390039 162 28 , , , 10_1101-2020_11_24_390039 162 29 who who WP 10_1101-2020_11_24_390039 162 30 has have VBZ 10_1101-2020_11_24_390039 162 31 granted grant VBN 10_1101-2020_11_24_390039 162 32 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 162 33 a a DT 10_1101-2020_11_24_390039 162 34 license license NN 10_1101-2020_11_24_390039 162 35 to to TO 10_1101-2020_11_24_390039 162 36 display display VB 10_1101-2020_11_24_390039 162 37 the the DT 10_1101-2020_11_24_390039 162 38 preprint preprint NN 10_1101-2020_11_24_390039 162 39 in in IN 10_1101-2020_11_24_390039 162 40 perpetuity perpetuity NN 10_1101-2020_11_24_390039 162 41 . . . 10_1101-2020_11_24_390039 163 1 It -PRON- PRP 10_1101-2020_11_24_390039 163 2 is be VBZ 10_1101-2020_11_24_390039 163 3 The the DT 10_1101-2020_11_24_390039 163 4 copyright copyright NN 10_1101-2020_11_24_390039 163 5 holder holder NN 10_1101-2020_11_24_390039 163 6 for for IN 10_1101-2020_11_24_390039 163 7 this this DT 10_1101-2020_11_24_390039 163 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 163 9 version version NN 10_1101-2020_11_24_390039 163 10 posted post VBD 10_1101-2020_11_24_390039 163 11 January January NNP 10_1101-2020_11_24_390039 163 12 5 5 CD 10_1101-2020_11_24_390039 163 13 , , , 10_1101-2020_11_24_390039 163 14 2021 2021 CD 10_1101-2020_11_24_390039 163 15 . . . 10_1101-2020_11_24_390039 163 16 ; ; : 10_1101-2020_11_24_390039 163 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 163 18 : : : 10_1101-2020_11_24_390039 163 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 163 20 preprint preprint JJ 10_1101-2020_11_24_390039 163 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 163 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 163 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 163 24 - - HYPH 10_1101-2020_11_24_390039 163 25 F F NNP 10_1101-2020_11_24_390039 163 26 as as IN 10_1101-2020_11_24_390039 163 27 a a DT 10_1101-2020_11_24_390039 163 28 potential potential JJ 10_1101-2020_11_24_390039 163 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 163 30 agent agent NN 10_1101-2020_11_24_390039 163 31 Page Page NNP 10_1101-2020_11_24_390039 163 32 13 13 CD 10_1101-2020_11_24_390039 163 33 of of IN 10_1101-2020_11_24_390039 163 34 21 21 CD 10_1101-2020_11_24_390039 163 35 fixed fix VBN 10_1101-2020_11_24_390039 163 36 for for IN 10_1101-2020_11_24_390039 163 37 5 5 CD 10_1101-2020_11_24_390039 163 38 min min NN 10_1101-2020_11_24_390039 163 39 ( ( -LRB- 10_1101-2020_11_24_390039 163 40 20 20 CD 10_1101-2020_11_24_390039 163 41 % % NN 10_1101-2020_11_24_390039 163 42 MeOH MeOH NNP 10_1101-2020_11_24_390039 163 43 , , , 10_1101-2020_11_24_390039 163 44 10 10 CD 10_1101-2020_11_24_390039 163 45 % % NN 10_1101-2020_11_24_390039 163 46 AcOH AcOH NNP 10_1101-2020_11_24_390039 163 47 ) ) -RRB- 10_1101-2020_11_24_390039 163 48 , , , 10_1101-2020_11_24_390039 163 49 dried dry VBN 10_1101-2020_11_24_390039 163 50 for for IN 10_1101-2020_11_24_390039 163 51 2 2 CD 10_1101-2020_11_24_390039 163 52 h h NN 10_1101-2020_11_24_390039 163 53 at at IN 10_1101-2020_11_24_390039 163 54 65 65 CD 10_1101-2020_11_24_390039 163 55 ° ° NNS 10_1101-2020_11_24_390039 163 56 C C NNP 10_1101-2020_11_24_390039 163 57 and and CC 10_1101-2020_11_24_390039 163 58 radiolabelled radiolabelle VBD 10_1101-2020_11_24_390039 163 59 products product NNS 10_1101-2020_11_24_390039 163 60 visualised visualise VBN 10_1101-2020_11_24_390039 163 61 using use VBG 10_1101-2020_11_24_390039 163 62 a a DT 10_1101-2020_11_24_390039 163 63 Typhoon Typhoon NNP 10_1101-2020_11_24_390039 163 64 FLA-700 fla-700 FW 10_1101-2020_11_24_390039 163 65 ( ( -LRB- 10_1101-2020_11_24_390039 163 66 GE GE NNP 10_1101-2020_11_24_390039 163 67 Healthcare Healthcare NNP 10_1101-2020_11_24_390039 163 68 ) ) -RRB- 10_1101-2020_11_24_390039 163 69 following follow VBG 10_1101-2020_11_24_390039 163 70 exposure exposure NN 10_1101-2020_11_24_390039 163 71 to to IN 10_1101-2020_11_24_390039 163 72 a a DT 10_1101-2020_11_24_390039 163 73 phosphorimaging phosphorimage VBG 10_1101-2020_11_24_390039 163 74 plate plate NN 10_1101-2020_11_24_390039 163 75 for for IN 10_1101-2020_11_24_390039 163 76 24 24 CD 10_1101-2020_11_24_390039 163 77 - - SYM 10_1101-2020_11_24_390039 163 78 72 72 CD 10_1101-2020_11_24_390039 163 79 h. h. NN 10_1101-2020_11_24_390039 163 80 Western Western NNP 10_1101-2020_11_24_390039 163 81 Blotting Blotting NNP 10_1101-2020_11_24_390039 163 82 Following follow VBG 10_1101-2020_11_24_390039 163 83 semi semi JJ 10_1101-2020_11_24_390039 163 84 - - NN 10_1101-2020_11_24_390039 163 85 permeabilisation permeabilisation NN 10_1101-2020_11_24_390039 163 86 , , , 10_1101-2020_11_24_390039 163 87 aliquots aliquot NNS 10_1101-2020_11_24_390039 163 88 of of IN 10_1101-2020_11_24_390039 163 89 siRNA sirna NN 10_1101-2020_11_24_390039 163 90 - - HYPH 10_1101-2020_11_24_390039 163 91 treated treat VBN 10_1101-2020_11_24_390039 163 92 HeLa HeLa NNP 10_1101-2020_11_24_390039 163 93 cells cell NNS 10_1101-2020_11_24_390039 163 94 were be VBD 10_1101-2020_11_24_390039 163 95 suspended suspend VBN 10_1101-2020_11_24_390039 163 96 in in IN 10_1101-2020_11_24_390039 163 97 SDS SDS NNP 10_1101-2020_11_24_390039 163 98 sample sample NN 10_1101-2020_11_24_390039 163 99 buffer buffer NN 10_1101-2020_11_24_390039 163 100 , , , 10_1101-2020_11_24_390039 163 101 denatured denature VBD 10_1101-2020_11_24_390039 163 102 for for IN 10_1101-2020_11_24_390039 163 103 12 12 CD 10_1101-2020_11_24_390039 163 104 h h NN 10_1101-2020_11_24_390039 163 105 at at IN 10_1101-2020_11_24_390039 163 106 37 37 CD 10_1101-2020_11_24_390039 163 107 ° ° NNS 10_1101-2020_11_24_390039 163 108 C C NNP 10_1101-2020_11_24_390039 163 109 and and CC 10_1101-2020_11_24_390039 163 110 sonicated sonicate VBN 10_1101-2020_11_24_390039 163 111 prior prior RB 10_1101-2020_11_24_390039 163 112 to to IN 10_1101-2020_11_24_390039 163 113 resolution resolution NN 10_1101-2020_11_24_390039 163 114 by by IN 10_1101-2020_11_24_390039 163 115 SDS SDS NNP 10_1101-2020_11_24_390039 163 116 - - HYPH 10_1101-2020_11_24_390039 163 117 PAGE PAGE NNP 10_1101-2020_11_24_390039 163 118 ( ( -LRB- 10_1101-2020_11_24_390039 163 119 16 16 CD 10_1101-2020_11_24_390039 163 120 % % NN 10_1101-2020_11_24_390039 163 121 or or CC 10_1101-2020_11_24_390039 163 122 10 10 CD 10_1101-2020_11_24_390039 163 123 % % NN 10_1101-2020_11_24_390039 163 124 PAGE PAGE NNP 10_1101-2020_11_24_390039 163 125 , , , 10_1101-2020_11_24_390039 163 126 120V 120v CD 10_1101-2020_11_24_390039 163 127 , , , 10_1101-2020_11_24_390039 163 128 120 120 CD 10_1101-2020_11_24_390039 163 129 - - SYM 10_1101-2020_11_24_390039 163 130 150 150 CD 10_1101-2020_11_24_390039 163 131 min min NN 10_1101-2020_11_24_390039 163 132 ) ) -RRB- 10_1101-2020_11_24_390039 163 133 . . . 10_1101-2020_11_24_390039 164 1 Following follow VBG 10_1101-2020_11_24_390039 164 2 transfer transfer NN 10_1101-2020_11_24_390039 164 3 to to IN 10_1101-2020_11_24_390039 164 4 a a DT 10_1101-2020_11_24_390039 164 5 PVDF PVDF NNP 10_1101-2020_11_24_390039 164 6 membrane membrane NN 10_1101-2020_11_24_390039 164 7 in in IN 10_1101-2020_11_24_390039 164 8 transfer transfer NN 10_1101-2020_11_24_390039 164 9 buffer buffer NN 10_1101-2020_11_24_390039 164 10 ( ( -LRB- 10_1101-2020_11_24_390039 164 11 0.06 0.06 CD 10_1101-2020_11_24_390039 164 12 M M NNP 10_1101-2020_11_24_390039 164 13 Tris Tris NNP 10_1101-2020_11_24_390039 164 14 , , , 10_1101-2020_11_24_390039 164 15 0.60 0.60 CD 10_1101-2020_11_24_390039 164 16 M M NNP 10_1101-2020_11_24_390039 164 17 glycine glycine NN 10_1101-2020_11_24_390039 164 18 , , , 10_1101-2020_11_24_390039 164 19 20 20 CD 10_1101-2020_11_24_390039 164 20 % % NN 10_1101-2020_11_24_390039 164 21 MeOH MeOH NNP 10_1101-2020_11_24_390039 164 22 ) ) -RRB- 10_1101-2020_11_24_390039 164 23 at at IN 10_1101-2020_11_24_390039 164 24 300 300 CD 10_1101-2020_11_24_390039 164 25 mA mA NNP 10_1101-2020_11_24_390039 164 26 for for IN 10_1101-2020_11_24_390039 164 27 2.5 2.5 CD 10_1101-2020_11_24_390039 164 28 h h NN 10_1101-2020_11_24_390039 164 29 , , , 10_1101-2020_11_24_390039 164 30 PVDF PVDF NNP 10_1101-2020_11_24_390039 164 31 membranes membrane NNS 10_1101-2020_11_24_390039 164 32 were be VBD 10_1101-2020_11_24_390039 164 33 incubated incubate VBN 10_1101-2020_11_24_390039 164 34 in in IN 10_1101-2020_11_24_390039 164 35 1X 1x JJ 10_1101-2020_11_24_390039 164 36 Casein Casein NNP 10_1101-2020_11_24_390039 164 37 blocking block VBG 10_1101-2020_11_24_390039 164 38 buffer buffer NN 10_1101-2020_11_24_390039 164 39 ( ( -LRB- 10_1101-2020_11_24_390039 164 40 10X 10x CD 10_1101-2020_11_24_390039 164 41 stock stock NN 10_1101-2020_11_24_390039 164 42 from from IN 10_1101-2020_11_24_390039 164 43 Sigma Sigma NNP 10_1101-2020_11_24_390039 164 44 - - HYPH 10_1101-2020_11_24_390039 164 45 Aldrich Aldrich NNP 10_1101-2020_11_24_390039 164 46 , , , 10_1101-2020_11_24_390039 164 47 B6429 B6429 NNP 10_1101-2020_11_24_390039 164 48 ) ) -RRB- 10_1101-2020_11_24_390039 164 49 made make VBD 10_1101-2020_11_24_390039 164 50 up up RP 10_1101-2020_11_24_390039 164 51 in in IN 10_1101-2020_11_24_390039 164 52 TBS TBS NNP 10_1101-2020_11_24_390039 164 53 , , , 10_1101-2020_11_24_390039 164 54 incubated incubate VBN 10_1101-2020_11_24_390039 164 55 with with IN 10_1101-2020_11_24_390039 164 56 appropriate appropriate JJ 10_1101-2020_11_24_390039 164 57 primary primary JJ 10_1101-2020_11_24_390039 164 58 antibodies antibody NNS 10_1101-2020_11_24_390039 164 59 ( ( -LRB- 10_1101-2020_11_24_390039 164 60 1:500 1:500 CD 10_1101-2020_11_24_390039 164 61 or or CC 10_1101-2020_11_24_390039 164 62 1:1000 1:1000 CD 10_1101-2020_11_24_390039 164 63 dilution dilution NN 10_1101-2020_11_24_390039 164 64 ) ) -RRB- 10_1101-2020_11_24_390039 164 65 and and CC 10_1101-2020_11_24_390039 164 66 processed process VBN 10_1101-2020_11_24_390039 164 67 for for IN 10_1101-2020_11_24_390039 164 68 fluorescence fluorescence NN 10_1101-2020_11_24_390039 164 69 - - HYPH 10_1101-2020_11_24_390039 164 70 based base VBN 10_1101-2020_11_24_390039 164 71 detection detection NN 10_1101-2020_11_24_390039 164 72 as as IN 10_1101-2020_11_24_390039 164 73 described describe VBN 10_1101-2020_11_24_390039 164 74 by by IN 10_1101-2020_11_24_390039 164 75 LI LI NNP 10_1101-2020_11_24_390039 164 76 - - HYPH 10_1101-2020_11_24_390039 164 77 COR COR NNP 10_1101-2020_11_24_390039 164 78 Biosciences Biosciences NNPS 10_1101-2020_11_24_390039 164 79 using use VBG 10_1101-2020_11_24_390039 164 80 appropriate appropriate JJ 10_1101-2020_11_24_390039 164 81 secondary secondary JJ 10_1101-2020_11_24_390039 164 82 antibodies antibody NNS 10_1101-2020_11_24_390039 164 83 ( ( -LRB- 10_1101-2020_11_24_390039 164 84 IRDye IRDye NNP 10_1101-2020_11_24_390039 164 85 680RD 680RD NNP 10_1101-2020_11_24_390039 164 86 Donkey Donkey NNP 10_1101-2020_11_24_390039 164 87 anti anti NNP 10_1101-2020_11_24_390039 164 88 - - NNP 10_1101-2020_11_24_390039 164 89 Goat Goat NNP 10_1101-2020_11_24_390039 164 90 , , , 10_1101-2020_11_24_390039 164 91 IRDye IRDye NNP 10_1101-2020_11_24_390039 164 92 680RD 680RD NNP 10_1101-2020_11_24_390039 164 93 Donkey Donkey NNP 10_1101-2020_11_24_390039 164 94 anti anti JJ 10_1101-2020_11_24_390039 164 95 - - NNP 10_1101-2020_11_24_390039 164 96 Rabbit rabbit JJ 10_1101-2020_11_24_390039 164 97 , , , 10_1101-2020_11_24_390039 164 98 IRDye IRDye NNP 10_1101-2020_11_24_390039 164 99 800CW 800CW NNP 10_1101-2020_11_24_390039 164 100 Donkey Donkey NNP 10_1101-2020_11_24_390039 164 101 anti anti NNP 10_1101-2020_11_24_390039 164 102 - - NNP 10_1101-2020_11_24_390039 164 103 Mouse Mouse NNP 10_1101-2020_11_24_390039 164 104 ) ) -RRB- 10_1101-2020_11_24_390039 164 105 at at IN 10_1101-2020_11_24_390039 164 106 1:10,000 1:10,000 CD 10_1101-2020_11_24_390039 164 107 dilution dilution NN 10_1101-2020_11_24_390039 164 108 . . . 10_1101-2020_11_24_390039 165 1 Signals signal NNS 10_1101-2020_11_24_390039 165 2 were be VBD 10_1101-2020_11_24_390039 165 3 visualised visualise VBN 10_1101-2020_11_24_390039 165 4 using use VBG 10_1101-2020_11_24_390039 165 5 an an DT 10_1101-2020_11_24_390039 165 6 Odyssey Odyssey NNP 10_1101-2020_11_24_390039 165 7 CLx CLx NNP 10_1101-2020_11_24_390039 165 8 Imaging Imaging NNP 10_1101-2020_11_24_390039 165 9 System System NNP 10_1101-2020_11_24_390039 165 10 ( ( -LRB- 10_1101-2020_11_24_390039 165 11 LI LI NNP 10_1101-2020_11_24_390039 165 12 - - HYPH 10_1101-2020_11_24_390039 165 13 COR COR NNP 10_1101-2020_11_24_390039 165 14 Biosciences Biosciences NNPS 10_1101-2020_11_24_390039 165 15 ) ) -RRB- 10_1101-2020_11_24_390039 165 16 . . . 10_1101-2020_11_24_390039 166 1 Quantitation quantitation NN 10_1101-2020_11_24_390039 166 2 and and CC 10_1101-2020_11_24_390039 166 3 Statistical Statistical NNP 10_1101-2020_11_24_390039 166 4 Analysis Analysis NNP 10_1101-2020_11_24_390039 166 5 Bar Bar NNP 10_1101-2020_11_24_390039 166 6 graphs graphs NN 10_1101-2020_11_24_390039 166 7 depict depict VBP 10_1101-2020_11_24_390039 166 8 either either CC 10_1101-2020_11_24_390039 166 9 the the DT 10_1101-2020_11_24_390039 166 10 efficiency efficiency NN 10_1101-2020_11_24_390039 166 11 of of IN 10_1101-2020_11_24_390039 166 12 membrane membrane NN 10_1101-2020_11_24_390039 166 13 translocation translocation NN 10_1101-2020_11_24_390039 166 14 / / SYM 10_1101-2020_11_24_390039 166 15 insertion insertion NN 10_1101-2020_11_24_390039 166 16 calculated calculate VBN 10_1101-2020_11_24_390039 166 17 as as IN 10_1101-2020_11_24_390039 166 18 the the DT 10_1101-2020_11_24_390039 166 19 ratio ratio NN 10_1101-2020_11_24_390039 166 20 of of IN 10_1101-2020_11_24_390039 166 21 N n NN 10_1101-2020_11_24_390039 166 22 - - HYPH 10_1101-2020_11_24_390039 166 23 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 166 24 protein protein NN 10_1101-2020_11_24_390039 166 25 relative relative JJ 10_1101-2020_11_24_390039 166 26 to to IN 10_1101-2020_11_24_390039 166 27 the the DT 10_1101-2020_11_24_390039 166 28 amount amount NN 10_1101-2020_11_24_390039 166 29 of of IN 10_1101-2020_11_24_390039 166 30 non non JJ 10_1101-2020_11_24_390039 166 31 - - JJ 10_1101-2020_11_24_390039 166 32 N- n- JJ 10_1101-2020_11_24_390039 166 33 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 166 34 protein protein NN 10_1101-2020_11_24_390039 166 35 ( ( -LRB- 10_1101-2020_11_24_390039 166 36 Fig fig NN 10_1101-2020_11_24_390039 166 37 . . . 10_1101-2020_11_24_390039 167 1 2 2 CD 10_1101-2020_11_24_390039 167 2 - - SYM 10_1101-2020_11_24_390039 167 3 3 3 CD 10_1101-2020_11_24_390039 167 4 ) ) -RRB- 10_1101-2020_11_24_390039 167 5 , , , 10_1101-2020_11_24_390039 167 6 or or CC 10_1101-2020_11_24_390039 167 7 the the DT 10_1101-2020_11_24_390039 167 8 efficiencies efficiency NNS 10_1101-2020_11_24_390039 167 9 of of IN 10_1101-2020_11_24_390039 167 10 siRNA sirna NN 10_1101-2020_11_24_390039 167 11 - - HYPH 10_1101-2020_11_24_390039 167 12 mediated mediate VBN 10_1101-2020_11_24_390039 167 13 knockdown knockdown VBN 10_1101-2020_11_24_390039 167 14 in in IN 10_1101-2020_11_24_390039 167 15 SP SP NNP 10_1101-2020_11_24_390039 167 16 cells cell NNS 10_1101-2020_11_24_390039 167 17 calculated calculate VBN 10_1101-2020_11_24_390039 167 18 as as IN 10_1101-2020_11_24_390039 167 19 a a DT 10_1101-2020_11_24_390039 167 20 proportion proportion NN 10_1101-2020_11_24_390039 167 21 of of IN 10_1101-2020_11_24_390039 167 22 the the DT 10_1101-2020_11_24_390039 167 23 protein protein NN 10_1101-2020_11_24_390039 167 24 content content NN 10_1101-2020_11_24_390039 167 25 when when WRB 10_1101-2020_11_24_390039 167 26 compared compare VBN 10_1101-2020_11_24_390039 167 27 to to IN 10_1101-2020_11_24_390039 167 28 the the DT 10_1101-2020_11_24_390039 167 29 NT NT NNP 10_1101-2020_11_24_390039 167 30 control control NN 10_1101-2020_11_24_390039 167 31 ( ( -LRB- 10_1101-2020_11_24_390039 167 32 Fig fig NN 10_1101-2020_11_24_390039 167 33 . . . 10_1101-2020_11_24_390039 168 1 S2 s2 NN 10_1101-2020_11_24_390039 168 2 ) ) -RRB- 10_1101-2020_11_24_390039 168 3 , , , 10_1101-2020_11_24_390039 168 4 with with IN 10_1101-2020_11_24_390039 168 5 all all DT 10_1101-2020_11_24_390039 168 6 control control NN 10_1101-2020_11_24_390039 168 7 samples sample NNS 10_1101-2020_11_24_390039 168 8 set set VBN 10_1101-2020_11_24_390039 168 9 to to IN 10_1101-2020_11_24_390039 168 10 100 100 CD 10_1101-2020_11_24_390039 168 11 % % NN 10_1101-2020_11_24_390039 168 12 . . . 10_1101-2020_11_24_390039 169 1 Normalised normalised JJ 10_1101-2020_11_24_390039 169 2 values value NNS 10_1101-2020_11_24_390039 169 3 were be VBD 10_1101-2020_11_24_390039 169 4 used use VBN 10_1101-2020_11_24_390039 169 5 for for IN 10_1101-2020_11_24_390039 169 6 statistical statistical JJ 10_1101-2020_11_24_390039 169 7 comparison comparison NN 10_1101-2020_11_24_390039 169 8 ( ( -LRB- 10_1101-2020_11_24_390039 169 9 one one CD 10_1101-2020_11_24_390039 169 10 - - HYPH 10_1101-2020_11_24_390039 169 11 way way NN 10_1101-2020_11_24_390039 169 12 or or CC 10_1101-2020_11_24_390039 169 13 two two CD 10_1101-2020_11_24_390039 169 14 - - HYPH 10_1101-2020_11_24_390039 169 15 way way NN 10_1101-2020_11_24_390039 169 16 ANOVA ANOVA NNP 10_1101-2020_11_24_390039 169 17 ; ; : 10_1101-2020_11_24_390039 169 18 DF DF NNP 10_1101-2020_11_24_390039 169 19 and and CC 10_1101-2020_11_24_390039 169 20 F F NNP 10_1101-2020_11_24_390039 169 21 values value NNS 10_1101-2020_11_24_390039 169 22 are be VBP 10_1101-2020_11_24_390039 169 23 shown show VBN 10_1101-2020_11_24_390039 169 24 in in IN 10_1101-2020_11_24_390039 169 25 each each DT 10_1101-2020_11_24_390039 169 26 figure figure NN 10_1101-2020_11_24_390039 169 27 as as IN 10_1101-2020_11_24_390039 169 28 appropriate appropriate JJ 10_1101-2020_11_24_390039 169 29 and and CC 10_1101-2020_11_24_390039 169 30 the the DT 10_1101-2020_11_24_390039 169 31 multiple multiple JJ 10_1101-2020_11_24_390039 169 32 comparisons comparison NNS 10_1101-2020_11_24_390039 169 33 test test NN 10_1101-2020_11_24_390039 169 34 used use VBN 10_1101-2020_11_24_390039 169 35 are be VBP 10_1101-2020_11_24_390039 169 36 indicated indicate VBN 10_1101-2020_11_24_390039 169 37 in in IN 10_1101-2020_11_24_390039 169 38 the the DT 10_1101-2020_11_24_390039 169 39 appropriate appropriate JJ 10_1101-2020_11_24_390039 169 40 figure figure NN 10_1101-2020_11_24_390039 169 41 legend legend NN 10_1101-2020_11_24_390039 169 42 ) ) -RRB- 10_1101-2020_11_24_390039 169 43 . . . 10_1101-2020_11_24_390039 170 1 Statistical statistical JJ 10_1101-2020_11_24_390039 170 2 significance significance NN 10_1101-2020_11_24_390039 170 3 is be VBZ 10_1101-2020_11_24_390039 170 4 given give VBN 10_1101-2020_11_24_390039 170 5 as as IN 10_1101-2020_11_24_390039 170 6 n.s n.s NNP 10_1101-2020_11_24_390039 170 7 . . NNP 10_1101-2020_11_24_390039 170 8 , , , 10_1101-2020_11_24_390039 170 9 non non JJ 10_1101-2020_11_24_390039 170 10 - - JJ 10_1101-2020_11_24_390039 170 11 significant significant JJ 10_1101-2020_11_24_390039 170 12 > > NN 10_1101-2020_11_24_390039 170 13 0.1 0.1 CD 10_1101-2020_11_24_390039 170 14 ; ; : 10_1101-2020_11_24_390039 170 15 * * NFP 10_1101-2020_11_24_390039 170 16 , , , 10_1101-2020_11_24_390039 170 17 P p NN 10_1101-2020_11_24_390039 170 18 < < XX 10_1101-2020_11_24_390039 170 19 0.05 0.05 XX 10_1101-2020_11_24_390039 170 20 ; ; : 10_1101-2020_11_24_390039 170 21 * * NFP 10_1101-2020_11_24_390039 170 22 * * NFP 10_1101-2020_11_24_390039 170 23 , , , 10_1101-2020_11_24_390039 170 24 P p NN 10_1101-2020_11_24_390039 170 25 < < XX 10_1101-2020_11_24_390039 170 26 0.01 0.01 XX 10_1101-2020_11_24_390039 170 27 ; ; : 10_1101-2020_11_24_390039 170 28 * * NFP 10_1101-2020_11_24_390039 170 29 * * NFP 10_1101-2020_11_24_390039 170 30 * * NFP 10_1101-2020_11_24_390039 170 31 , , , 10_1101-2020_11_24_390039 170 32 P p NN 10_1101-2020_11_24_390039 170 33 < < XX 10_1101-2020_11_24_390039 170 34 0.001 0.001 CD 10_1101-2020_11_24_390039 170 35 ; ; : 10_1101-2020_11_24_390039 170 36 * * NFP 10_1101-2020_11_24_390039 170 37 * * NFP 10_1101-2020_11_24_390039 170 38 * * NFP 10_1101-2020_11_24_390039 170 39 * * NFP 10_1101-2020_11_24_390039 170 40 , , , 10_1101-2020_11_24_390039 170 41 P p NN 10_1101-2020_11_24_390039 170 42 < < XX 10_1101-2020_11_24_390039 170 43 0.0001 0.0001 CD 10_1101-2020_11_24_390039 170 44 . . . 10_1101-2020_11_24_390039 171 1 .CC .CC NFP 10_1101-2020_11_24_390039 171 2 - - : 10_1101-2020_11_24_390039 171 3 BY by IN 10_1101-2020_11_24_390039 171 4 - - HYPH 10_1101-2020_11_24_390039 171 5 ND ND NNP 10_1101-2020_11_24_390039 171 6 4.0 4.0 CD 10_1101-2020_11_24_390039 171 7 International international JJ 10_1101-2020_11_24_390039 171 8 licensemade licensemade NN 10_1101-2020_11_24_390039 171 9 available available JJ 10_1101-2020_11_24_390039 171 10 under under IN 10_1101-2020_11_24_390039 171 11 a a DT 10_1101-2020_11_24_390039 171 12 ( ( -LRB- 10_1101-2020_11_24_390039 171 13 which which WDT 10_1101-2020_11_24_390039 171 14 was be VBD 10_1101-2020_11_24_390039 171 15 not not RB 10_1101-2020_11_24_390039 171 16 certified certify VBN 10_1101-2020_11_24_390039 171 17 by by IN 10_1101-2020_11_24_390039 171 18 peer peer NN 10_1101-2020_11_24_390039 171 19 review review NN 10_1101-2020_11_24_390039 171 20 ) ) -RRB- 10_1101-2020_11_24_390039 171 21 is be VBZ 10_1101-2020_11_24_390039 171 22 the the DT 10_1101-2020_11_24_390039 171 23 author author NN 10_1101-2020_11_24_390039 171 24 / / SYM 10_1101-2020_11_24_390039 171 25 funder funder NN 10_1101-2020_11_24_390039 171 26 , , , 10_1101-2020_11_24_390039 171 27 who who WP 10_1101-2020_11_24_390039 171 28 has have VBZ 10_1101-2020_11_24_390039 171 29 granted grant VBN 10_1101-2020_11_24_390039 171 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 171 31 a a DT 10_1101-2020_11_24_390039 171 32 license license NN 10_1101-2020_11_24_390039 171 33 to to TO 10_1101-2020_11_24_390039 171 34 display display VB 10_1101-2020_11_24_390039 171 35 the the DT 10_1101-2020_11_24_390039 171 36 preprint preprint NN 10_1101-2020_11_24_390039 171 37 in in IN 10_1101-2020_11_24_390039 171 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 171 39 . . . 10_1101-2020_11_24_390039 172 1 It -PRON- PRP 10_1101-2020_11_24_390039 172 2 is be VBZ 10_1101-2020_11_24_390039 172 3 The the DT 10_1101-2020_11_24_390039 172 4 copyright copyright NN 10_1101-2020_11_24_390039 172 5 holder holder NN 10_1101-2020_11_24_390039 172 6 for for IN 10_1101-2020_11_24_390039 172 7 this this DT 10_1101-2020_11_24_390039 172 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 172 9 version version NN 10_1101-2020_11_24_390039 172 10 posted post VBD 10_1101-2020_11_24_390039 172 11 January January NNP 10_1101-2020_11_24_390039 172 12 5 5 CD 10_1101-2020_11_24_390039 172 13 , , , 10_1101-2020_11_24_390039 172 14 2021 2021 CD 10_1101-2020_11_24_390039 172 15 . . . 10_1101-2020_11_24_390039 172 16 ; ; : 10_1101-2020_11_24_390039 172 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 172 18 : : : 10_1101-2020_11_24_390039 172 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 172 20 preprint preprint JJ 10_1101-2020_11_24_390039 172 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 172 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 172 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 172 24 - - HYPH 10_1101-2020_11_24_390039 172 25 F F NNP 10_1101-2020_11_24_390039 172 26 as as IN 10_1101-2020_11_24_390039 172 27 a a DT 10_1101-2020_11_24_390039 172 28 potential potential JJ 10_1101-2020_11_24_390039 172 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 172 30 agent agent NN 10_1101-2020_11_24_390039 172 31 Page Page NNP 10_1101-2020_11_24_390039 172 32 14 14 CD 10_1101-2020_11_24_390039 172 33 of of IN 10_1101-2020_11_24_390039 172 34 21 21 CD 10_1101-2020_11_24_390039 172 35 Acknowledgements Acknowledgements NNPS 10_1101-2020_11_24_390039 172 36 We -PRON- PRP 10_1101-2020_11_24_390039 172 37 thank thank VBP 10_1101-2020_11_24_390039 172 38 Quentin Quentin NNP 10_1101-2020_11_24_390039 172 39 Roebuck Roebuck NNP 10_1101-2020_11_24_390039 172 40 for for IN 10_1101-2020_11_24_390039 172 41 technical technical JJ 10_1101-2020_11_24_390039 172 42 assistance assistance NN 10_1101-2020_11_24_390039 172 43 , , , 10_1101-2020_11_24_390039 172 44 Nevan Nevan NNP 10_1101-2020_11_24_390039 172 45 Krogan Krogan NNP 10_1101-2020_11_24_390039 172 46 ( ( -LRB- 10_1101-2020_11_24_390039 172 47 UCSF UCSF NNP 10_1101-2020_11_24_390039 172 48 ) ) -RRB- 10_1101-2020_11_24_390039 172 49 for for IN 10_1101-2020_11_24_390039 172 50 SARS SARS NNP 10_1101-2020_11_24_390039 172 51 - - HYPH 10_1101-2020_11_24_390039 172 52 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 172 53 plasmids plasmid NNS 10_1101-2020_11_24_390039 172 54 , , , 10_1101-2020_11_24_390039 172 55 Sven Sven NNP 10_1101-2020_11_24_390039 172 56 Lang Lang NNP 10_1101-2020_11_24_390039 172 57 ( ( -LRB- 10_1101-2020_11_24_390039 172 58 University University NNP 10_1101-2020_11_24_390039 172 59 of of IN 10_1101-2020_11_24_390039 172 60 Saarland Saarland NNP 10_1101-2020_11_24_390039 172 61 ) ) -RRB- 10_1101-2020_11_24_390039 172 62 for for IN 10_1101-2020_11_24_390039 172 63 Sec61α Sec61α NNP 10_1101-2020_11_24_390039 172 64 antisera antisera NNP 10_1101-2020_11_24_390039 172 65 , , , 10_1101-2020_11_24_390039 172 66 Belinda Belinda NNP 10_1101-2020_11_24_390039 172 67 Hall Hall NNP 10_1101-2020_11_24_390039 172 68 and and CC 10_1101-2020_11_24_390039 172 69 Rachel Rachel NNP 10_1101-2020_11_24_390039 172 70 Simmonds Simmonds NNP 10_1101-2020_11_24_390039 172 71 ( ( -LRB- 10_1101-2020_11_24_390039 172 72 University University NNP 10_1101-2020_11_24_390039 172 73 of of IN 10_1101-2020_11_24_390039 172 74 Surrey Surrey NNP 10_1101-2020_11_24_390039 172 75 ) ) -RRB- 10_1101-2020_11_24_390039 172 76 for for IN 10_1101-2020_11_24_390039 172 77 useful useful JJ 10_1101-2020_11_24_390039 172 78 discussions discussion NNS 10_1101-2020_11_24_390039 172 79 . . . 10_1101-2020_11_24_390039 173 1 We -PRON- PRP 10_1101-2020_11_24_390039 173 2 are be VBP 10_1101-2020_11_24_390039 173 3 indebted indebted JJ 10_1101-2020_11_24_390039 173 4 to to IN 10_1101-2020_11_24_390039 173 5 Richard Richard NNP 10_1101-2020_11_24_390039 173 6 Zimmermann Zimmermann NNP 10_1101-2020_11_24_390039 173 7 ( ( -LRB- 10_1101-2020_11_24_390039 173 8 University University NNP 10_1101-2020_11_24_390039 173 9 of of IN 10_1101-2020_11_24_390039 173 10 Saarland Saarland NNP 10_1101-2020_11_24_390039 173 11 ) ) -RRB- 10_1101-2020_11_24_390039 173 12 for for IN 10_1101-2020_11_24_390039 173 13 catalyzing catalyze VBG 10_1101-2020_11_24_390039 173 14 SARS SARS NNP 10_1101-2020_11_24_390039 173 15 - - HYPH 10_1101-2020_11_24_390039 173 16 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 173 17 related related JJ 10_1101-2020_11_24_390039 173 18 discussions discussion NNS 10_1101-2020_11_24_390039 173 19 amongst amongst IN 10_1101-2020_11_24_390039 173 20 the the DT 10_1101-2020_11_24_390039 173 21 ER ER NNP 10_1101-2020_11_24_390039 173 22 research research NN 10_1101-2020_11_24_390039 173 23 community community NN 10_1101-2020_11_24_390039 173 24 . . . 10_1101-2020_11_24_390039 174 1 Competing compete VBG 10_1101-2020_11_24_390039 174 2 interests interest NNS 10_1101-2020_11_24_390039 174 3 The the DT 10_1101-2020_11_24_390039 174 4 authors author NNS 10_1101-2020_11_24_390039 174 5 declare declare VBP 10_1101-2020_11_24_390039 174 6 no no DT 10_1101-2020_11_24_390039 174 7 competing compete VBG 10_1101-2020_11_24_390039 174 8 interests interest NNS 10_1101-2020_11_24_390039 174 9 . . . 10_1101-2020_11_24_390039 175 1 Author author NN 10_1101-2020_11_24_390039 175 2 Contributions Contributions NNP 10_1101-2020_11_24_390039 175 3 K.B.D. K.B.D. NNP 10_1101-2020_11_24_390039 175 4 , , , 10_1101-2020_11_24_390039 175 5 G.Z. G.Z. NNP 10_1101-2020_11_24_390039 176 1 and and CC 10_1101-2020_11_24_390039 176 2 H.S. H.S. NNP 10_1101-2020_11_24_390039 177 1 participated participate VBN 10_1101-2020_11_24_390039 177 2 in in IN 10_1101-2020_11_24_390039 177 3 synthesis synthesis NN 10_1101-2020_11_24_390039 177 4 of of IN 10_1101-2020_11_24_390039 177 5 Ipom Ipom NNP 10_1101-2020_11_24_390039 177 6 - - HYPH 10_1101-2020_11_24_390039 177 7 F F NNP 10_1101-2020_11_24_390039 177 8 and and CC 10_1101-2020_11_24_390039 177 9 W.Q.S W.Q.S NNP 10_1101-2020_11_24_390039 177 10 supervised supervise VBD 10_1101-2020_11_24_390039 177 11 the the DT 10_1101-2020_11_24_390039 177 12 synthesis synthesis NN 10_1101-2020_11_24_390039 177 13 ; ; : 10_1101-2020_11_24_390039 177 14 P.R. P.R. NNP 10_1101-2020_11_24_390039 178 1 generated generate VBN 10_1101-2020_11_24_390039 178 2 SARS SARS NNP 10_1101-2020_11_24_390039 178 3 - - HYPH 10_1101-2020_11_24_390039 178 4 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 178 5 plasmids plasmid NNS 10_1101-2020_11_24_390039 178 6 ; ; : 10_1101-2020_11_24_390039 178 7 S.O’K. s.o’k. ADD 10_1101-2020_11_24_390039 179 1 performed perform VBN 10_1101-2020_11_24_390039 179 2 site- site- NNP 10_1101-2020_11_24_390039 179 3 directed direct VBD 10_1101-2020_11_24_390039 179 4 mutagenesis mutagenesis NN 10_1101-2020_11_24_390039 179 5 and and CC 10_1101-2020_11_24_390039 179 6 experiments experiment NNS 10_1101-2020_11_24_390039 179 7 ; ; : 10_1101-2020_11_24_390039 179 8 S.O’K. s.o’k. ADD 10_1101-2020_11_24_390039 180 1 and and CC 10_1101-2020_11_24_390039 180 2 S.H. S.H. NNP 10_1101-2020_11_24_390039 181 1 designed design VBN 10_1101-2020_11_24_390039 181 2 the the DT 10_1101-2020_11_24_390039 181 3 study study NN 10_1101-2020_11_24_390039 181 4 , , , 10_1101-2020_11_24_390039 181 5 analysed analyse VBD 10_1101-2020_11_24_390039 181 6 the the DT 10_1101-2020_11_24_390039 181 7 data datum NNS 10_1101-2020_11_24_390039 181 8 and and CC 10_1101-2020_11_24_390039 181 9 wrote write VBD 10_1101-2020_11_24_390039 181 10 the the DT 10_1101-2020_11_24_390039 181 11 manuscript manuscript NN 10_1101-2020_11_24_390039 181 12 . . . 10_1101-2020_11_24_390039 182 1 Funding fund VBG 10_1101-2020_11_24_390039 182 2 This this DT 10_1101-2020_11_24_390039 182 3 work work NN 10_1101-2020_11_24_390039 182 4 was be VBD 10_1101-2020_11_24_390039 182 5 supported support VBN 10_1101-2020_11_24_390039 182 6 by by IN 10_1101-2020_11_24_390039 182 7 a a DT 10_1101-2020_11_24_390039 182 8 Wellcome Wellcome NNP 10_1101-2020_11_24_390039 182 9 Trust Trust NNP 10_1101-2020_11_24_390039 182 10 Investigator Investigator NNP 10_1101-2020_11_24_390039 182 11 Award Award NNP 10_1101-2020_11_24_390039 182 12 in in IN 10_1101-2020_11_24_390039 182 13 Science Science NNP 10_1101-2020_11_24_390039 182 14 204957 204957 CD 10_1101-2020_11_24_390039 182 15 / / SYM 10_1101-2020_11_24_390039 182 16 Z/16 Z/16 NNP 10_1101-2020_11_24_390039 182 17 / / SYM 10_1101-2020_11_24_390039 182 18 Z Z NNP 10_1101-2020_11_24_390039 182 19 ( ( -LRB- 10_1101-2020_11_24_390039 182 20 S.H. S.H. NNP 10_1101-2020_11_24_390039 183 1 ) ) -RRB- 10_1101-2020_11_24_390039 183 2 , , , 10_1101-2020_11_24_390039 183 3 an an DT 10_1101-2020_11_24_390039 183 4 AREA area NN 10_1101-2020_11_24_390039 183 5 grant grant NN 10_1101-2020_11_24_390039 183 6 2R15GM116032 2r15gm116032 CD 10_1101-2020_11_24_390039 183 7 - - : 10_1101-2020_11_24_390039 183 8 02A1 02a1 CD 10_1101-2020_11_24_390039 183 9 from from IN 10_1101-2020_11_24_390039 183 10 the the DT 10_1101-2020_11_24_390039 183 11 National National NNP 10_1101-2020_11_24_390039 183 12 Institute Institute NNP 10_1101-2020_11_24_390039 183 13 of of IN 10_1101-2020_11_24_390039 183 14 General General NNP 10_1101-2020_11_24_390039 183 15 Medical Medical NNP 10_1101-2020_11_24_390039 183 16 Sciences Sciences NNPS 10_1101-2020_11_24_390039 183 17 of of IN 10_1101-2020_11_24_390039 183 18 the the DT 10_1101-2020_11_24_390039 183 19 National National NNP 10_1101-2020_11_24_390039 183 20 Institutes Institutes NNPS 10_1101-2020_11_24_390039 183 21 of of IN 10_1101-2020_11_24_390039 183 22 Health Health NNP 10_1101-2020_11_24_390039 183 23 ( ( -LRB- 10_1101-2020_11_24_390039 183 24 NIH NIH NNP 10_1101-2020_11_24_390039 183 25 ) ) -RRB- 10_1101-2020_11_24_390039 183 26 and and CC 10_1101-2020_11_24_390039 183 27 a a DT 10_1101-2020_11_24_390039 183 28 Ball Ball NNP 10_1101-2020_11_24_390039 183 29 State State NNP 10_1101-2020_11_24_390039 183 30 University University NNP 10_1101-2020_11_24_390039 183 31 ( ( -LRB- 10_1101-2020_11_24_390039 183 32 BSU BSU NNP 10_1101-2020_11_24_390039 183 33 ) ) -RRB- 10_1101-2020_11_24_390039 183 34 Provost Provost NNP 10_1101-2020_11_24_390039 183 35 Startup Startup NNP 10_1101-2020_11_24_390039 183 36 Award Award NNP 10_1101-2020_11_24_390039 183 37 ( ( -LRB- 10_1101-2020_11_24_390039 183 38 W.Q.S. W.Q.S. NNP 10_1101-2020_11_24_390039 183 39 ) ) -RRB- 10_1101-2020_11_24_390039 183 40 . . . 10_1101-2020_11_24_390039 184 1 Supplementary supplementary JJ 10_1101-2020_11_24_390039 184 2 Information Information NNP 10_1101-2020_11_24_390039 184 3 Supplementary Supplementary NNP 10_1101-2020_11_24_390039 184 4 information information NN 10_1101-2020_11_24_390039 184 5 Fig Fig NNP 10_1101-2020_11_24_390039 184 6 . . . 10_1101-2020_11_24_390039 185 1 S1 S1 NNP 10_1101-2020_11_24_390039 185 2 and and CC 10_1101-2020_11_24_390039 185 3 Fig Fig NNP 10_1101-2020_11_24_390039 185 4 . . . 10_1101-2020_11_24_390039 186 1 S2 s2 NN 10_1101-2020_11_24_390039 186 2 accompanies accompany VBZ 10_1101-2020_11_24_390039 186 3 this this DT 10_1101-2020_11_24_390039 186 4 report report NN 10_1101-2020_11_24_390039 186 5 . . . 10_1101-2020_11_24_390039 187 1 .CC .CC NFP 10_1101-2020_11_24_390039 187 2 - - : 10_1101-2020_11_24_390039 187 3 BY by IN 10_1101-2020_11_24_390039 187 4 - - HYPH 10_1101-2020_11_24_390039 187 5 ND ND NNP 10_1101-2020_11_24_390039 187 6 4.0 4.0 CD 10_1101-2020_11_24_390039 187 7 International international JJ 10_1101-2020_11_24_390039 187 8 licensemade licensemade NN 10_1101-2020_11_24_390039 187 9 available available JJ 10_1101-2020_11_24_390039 187 10 under under IN 10_1101-2020_11_24_390039 187 11 a a DT 10_1101-2020_11_24_390039 187 12 ( ( -LRB- 10_1101-2020_11_24_390039 187 13 which which WDT 10_1101-2020_11_24_390039 187 14 was be VBD 10_1101-2020_11_24_390039 187 15 not not RB 10_1101-2020_11_24_390039 187 16 certified certify VBN 10_1101-2020_11_24_390039 187 17 by by IN 10_1101-2020_11_24_390039 187 18 peer peer NN 10_1101-2020_11_24_390039 187 19 review review NN 10_1101-2020_11_24_390039 187 20 ) ) -RRB- 10_1101-2020_11_24_390039 187 21 is be VBZ 10_1101-2020_11_24_390039 187 22 the the DT 10_1101-2020_11_24_390039 187 23 author author NN 10_1101-2020_11_24_390039 187 24 / / SYM 10_1101-2020_11_24_390039 187 25 funder funder NN 10_1101-2020_11_24_390039 187 26 , , , 10_1101-2020_11_24_390039 187 27 who who WP 10_1101-2020_11_24_390039 187 28 has have VBZ 10_1101-2020_11_24_390039 187 29 granted grant VBN 10_1101-2020_11_24_390039 187 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 187 31 a a DT 10_1101-2020_11_24_390039 187 32 license license NN 10_1101-2020_11_24_390039 187 33 to to TO 10_1101-2020_11_24_390039 187 34 display display VB 10_1101-2020_11_24_390039 187 35 the the DT 10_1101-2020_11_24_390039 187 36 preprint preprint NN 10_1101-2020_11_24_390039 187 37 in in IN 10_1101-2020_11_24_390039 187 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 187 39 . . . 10_1101-2020_11_24_390039 188 1 It -PRON- PRP 10_1101-2020_11_24_390039 188 2 is be VBZ 10_1101-2020_11_24_390039 188 3 The the DT 10_1101-2020_11_24_390039 188 4 copyright copyright NN 10_1101-2020_11_24_390039 188 5 holder holder NN 10_1101-2020_11_24_390039 188 6 for for IN 10_1101-2020_11_24_390039 188 7 this this DT 10_1101-2020_11_24_390039 188 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 188 9 version version NN 10_1101-2020_11_24_390039 188 10 posted post VBD 10_1101-2020_11_24_390039 188 11 January January NNP 10_1101-2020_11_24_390039 188 12 5 5 CD 10_1101-2020_11_24_390039 188 13 , , , 10_1101-2020_11_24_390039 188 14 2021 2021 CD 10_1101-2020_11_24_390039 188 15 . . . 10_1101-2020_11_24_390039 188 16 ; ; : 10_1101-2020_11_24_390039 188 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 188 18 : : : 10_1101-2020_11_24_390039 188 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 188 20 preprint preprint JJ 10_1101-2020_11_24_390039 188 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 188 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 188 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 188 24 - - HYPH 10_1101-2020_11_24_390039 188 25 F F NNP 10_1101-2020_11_24_390039 188 26 as as IN 10_1101-2020_11_24_390039 188 27 a a DT 10_1101-2020_11_24_390039 188 28 potential potential JJ 10_1101-2020_11_24_390039 188 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 188 30 agent agent NN 10_1101-2020_11_24_390039 188 31 Page Page NNP 10_1101-2020_11_24_390039 188 32 15 15 CD 10_1101-2020_11_24_390039 188 33 of of IN 10_1101-2020_11_24_390039 188 34 21 21 CD 10_1101-2020_11_24_390039 188 35 References References NNPS 10_1101-2020_11_24_390039 188 36 Adalja Adalja NNP 10_1101-2020_11_24_390039 188 37 , , , 10_1101-2020_11_24_390039 188 38 A. a. NN 10_1101-2020_11_24_390039 188 39 , , , 10_1101-2020_11_24_390039 188 40 and and CC 10_1101-2020_11_24_390039 188 41 Inglesby Inglesby NNP 10_1101-2020_11_24_390039 188 42 , , , 10_1101-2020_11_24_390039 188 43 T. t. NN 10_1101-2020_11_24_390039 188 44 ( ( -LRB- 10_1101-2020_11_24_390039 188 45 2019 2019 CD 10_1101-2020_11_24_390039 188 46 ) ) -RRB- 10_1101-2020_11_24_390039 188 47 . . . 10_1101-2020_11_24_390039 189 1 Broad broad JJ 10_1101-2020_11_24_390039 189 2 - - HYPH 10_1101-2020_11_24_390039 189 3 spectrum spectrum NN 10_1101-2020_11_24_390039 189 4 antiviral antiviral JJ 10_1101-2020_11_24_390039 189 5 agents agent NNS 10_1101-2020_11_24_390039 189 6 : : : 10_1101-2020_11_24_390039 189 7 A a DT 10_1101-2020_11_24_390039 189 8 crucial crucial JJ 10_1101-2020_11_24_390039 189 9 pandemic pandemic JJ 10_1101-2020_11_24_390039 189 10 tool tool NN 10_1101-2020_11_24_390039 189 11 . . . 10_1101-2020_11_24_390039 190 1 Exp Exp NNP 10_1101-2020_11_24_390039 190 2 . . . 10_1101-2020_11_24_390039 191 1 Rev. Rev. NNP 10_1101-2020_11_24_390039 192 1 Anti Anti NNP 10_1101-2020_11_24_390039 192 2 - - NNP 10_1101-2020_11_24_390039 192 3 Infect Infect NNP 10_1101-2020_11_24_390039 192 4 . . . 10_1101-2020_11_24_390039 193 1 Ther ther RB 10_1101-2020_11_24_390039 193 2 . . . 10_1101-2020_11_24_390039 194 1 17 17 CD 10_1101-2020_11_24_390039 194 2 , , , 10_1101-2020_11_24_390039 194 3 467 467 CD 10_1101-2020_11_24_390039 194 4 - - SYM 10_1101-2020_11_24_390039 194 5 470 470 CD 10_1101-2020_11_24_390039 194 6 . . . 10_1101-2020_11_24_390039 195 1 Bojkova Bojkova NNP 10_1101-2020_11_24_390039 195 2 , , , 10_1101-2020_11_24_390039 195 3 D. D. NNP 10_1101-2020_11_24_390039 195 4 , , , 10_1101-2020_11_24_390039 195 5 Klann Klann NNP 10_1101-2020_11_24_390039 195 6 , , , 10_1101-2020_11_24_390039 195 7 K. K. NNP 10_1101-2020_11_24_390039 195 8 , , , 10_1101-2020_11_24_390039 195 9 Koch Koch NNP 10_1101-2020_11_24_390039 195 10 , , , 10_1101-2020_11_24_390039 195 11 B. B. NNP 10_1101-2020_11_24_390039 195 12 , , , 10_1101-2020_11_24_390039 195 13 Widera Widera NNP 10_1101-2020_11_24_390039 195 14 , , , 10_1101-2020_11_24_390039 195 15 M. M. NNP 10_1101-2020_11_24_390039 195 16 , , , 10_1101-2020_11_24_390039 195 17 Krause Krause NNP 10_1101-2020_11_24_390039 195 18 , , , 10_1101-2020_11_24_390039 195 19 D. D. NNP 10_1101-2020_11_24_390039 195 20 , , , 10_1101-2020_11_24_390039 195 21 Ciesek Ciesek NNP 10_1101-2020_11_24_390039 195 22 , , , 10_1101-2020_11_24_390039 195 23 S. S. NNP 10_1101-2020_11_24_390039 195 24 , , , 10_1101-2020_11_24_390039 195 25 Cinatl Cinatl NNP 10_1101-2020_11_24_390039 195 26 , , , 10_1101-2020_11_24_390039 195 27 J. J. NNP 10_1101-2020_11_24_390039 195 28 , , , 10_1101-2020_11_24_390039 195 29 and and CC 10_1101-2020_11_24_390039 195 30 Münch Münch NNP 10_1101-2020_11_24_390039 195 31 , , , 10_1101-2020_11_24_390039 195 32 C. C. NNP 10_1101-2020_11_24_390039 195 33 ( ( -LRB- 10_1101-2020_11_24_390039 195 34 2020 2020 CD 10_1101-2020_11_24_390039 195 35 ) ) -RRB- 10_1101-2020_11_24_390039 195 36 . . . 10_1101-2020_11_24_390039 196 1 Proteomics Proteomics NNP 10_1101-2020_11_24_390039 196 2 of of IN 10_1101-2020_11_24_390039 196 3 SARS SARS NNP 10_1101-2020_11_24_390039 196 4 - - HYPH 10_1101-2020_11_24_390039 196 5 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 196 6 infected infected JJ 10_1101-2020_11_24_390039 196 7 host host NN 10_1101-2020_11_24_390039 196 8 cells cell NNS 10_1101-2020_11_24_390039 196 9 reveals reveal VBZ 10_1101-2020_11_24_390039 196 10 therapy therapy NN 10_1101-2020_11_24_390039 196 11 targets target NNS 10_1101-2020_11_24_390039 196 12 . . . 10_1101-2020_11_24_390039 197 1 Nature nature NN 10_1101-2020_11_24_390039 197 2 . . . 10_1101-2020_11_24_390039 198 1 583 583 CD 10_1101-2020_11_24_390039 198 2 , , , 10_1101-2020_11_24_390039 198 3 469 469 CD 10_1101-2020_11_24_390039 198 4 - - SYM 10_1101-2020_11_24_390039 198 5 472 472 CD 10_1101-2020_11_24_390039 198 6 . . . 10_1101-2020_11_24_390039 199 1 Cantuti Cantuti NNP 10_1101-2020_11_24_390039 199 2 - - HYPH 10_1101-2020_11_24_390039 199 3 Castelvetri Castelvetri NNP 10_1101-2020_11_24_390039 199 4 , , , 10_1101-2020_11_24_390039 199 5 L. L. NNP 10_1101-2020_11_24_390039 199 6 , , , 10_1101-2020_11_24_390039 199 7 Ojha Ojha NNP 10_1101-2020_11_24_390039 199 8 , , , 10_1101-2020_11_24_390039 199 9 R. R. NNP 10_1101-2020_11_24_390039 199 10 , , , 10_1101-2020_11_24_390039 199 11 Pedro Pedro NNP 10_1101-2020_11_24_390039 199 12 , , , 10_1101-2020_11_24_390039 199 13 L. L. NNP 10_1101-2020_11_24_390039 199 14 D. D. NNP 10_1101-2020_11_24_390039 199 15 , , , 10_1101-2020_11_24_390039 199 16 Djannatian Djannatian NNP 10_1101-2020_11_24_390039 199 17 , , , 10_1101-2020_11_24_390039 199 18 M. M. NNP 10_1101-2020_11_24_390039 199 19 , , , 10_1101-2020_11_24_390039 199 20 Franz Franz NNP 10_1101-2020_11_24_390039 199 21 , , , 10_1101-2020_11_24_390039 199 22 J. J. NNP 10_1101-2020_11_24_390039 199 23 , , , 10_1101-2020_11_24_390039 199 24 Kuivanen Kuivanen NNP 10_1101-2020_11_24_390039 199 25 , , , 10_1101-2020_11_24_390039 199 26 S. S. NNP 10_1101-2020_11_24_390039 199 27 , , , 10_1101-2020_11_24_390039 199 28 van van NNP 10_1101-2020_11_24_390039 199 29 der der NNP 10_1101-2020_11_24_390039 199 30 Meer Meer NNP 10_1101-2020_11_24_390039 199 31 , , , 10_1101-2020_11_24_390039 199 32 F. F. NNP 10_1101-2020_11_24_390039 199 33 , , , 10_1101-2020_11_24_390039 199 34 Kallio Kallio NNP 10_1101-2020_11_24_390039 199 35 , , , 10_1101-2020_11_24_390039 199 36 K. K. NNP 10_1101-2020_11_24_390039 199 37 , , , 10_1101-2020_11_24_390039 199 38 Kaya Kaya NNP 10_1101-2020_11_24_390039 199 39 , , , 10_1101-2020_11_24_390039 199 40 T. T. NNP 10_1101-2020_11_24_390039 199 41 , , , 10_1101-2020_11_24_390039 199 42 Anastasina Anastasina NNP 10_1101-2020_11_24_390039 199 43 , , , 10_1101-2020_11_24_390039 199 44 M. M. NNP 10_1101-2020_11_24_390039 199 45 , , , 10_1101-2020_11_24_390039 199 46 et et NNP 10_1101-2020_11_24_390039 199 47 al al NNP 10_1101-2020_11_24_390039 199 48 . . . 10_1101-2020_11_24_390039 200 1 ( ( -LRB- 10_1101-2020_11_24_390039 200 2 2020 2020 CD 10_1101-2020_11_24_390039 200 3 ) ) -RRB- 10_1101-2020_11_24_390039 200 4 . . . 10_1101-2020_11_24_390039 201 1 Neuropilin-1 Neuropilin-1 NNP 10_1101-2020_11_24_390039 201 2 facilitates facilitate VBZ 10_1101-2020_11_24_390039 201 3 SARS SARS NNP 10_1101-2020_11_24_390039 201 4 - - HYPH 10_1101-2020_11_24_390039 201 5 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 201 6 cell cell NN 10_1101-2020_11_24_390039 201 7 entry entry NN 10_1101-2020_11_24_390039 201 8 and and CC 10_1101-2020_11_24_390039 201 9 infectivity infectivity NN 10_1101-2020_11_24_390039 201 10 . . . 10_1101-2020_11_24_390039 202 1 Science science NN 10_1101-2020_11_24_390039 202 2 . . . 10_1101-2020_11_24_390039 203 1 370 370 CD 10_1101-2020_11_24_390039 203 2 , , , 10_1101-2020_11_24_390039 203 3 856 856 CD 10_1101-2020_11_24_390039 203 4 - - SYM 10_1101-2020_11_24_390039 203 5 860 860 CD 10_1101-2020_11_24_390039 203 6 . . . 10_1101-2020_11_24_390039 204 1 Chitwood Chitwood NNP 10_1101-2020_11_24_390039 204 2 , , , 10_1101-2020_11_24_390039 204 3 P. P. NNP 10_1101-2020_11_24_390039 204 4 J. J. NNP 10_1101-2020_11_24_390039 204 5 , , , 10_1101-2020_11_24_390039 204 6 Juszkiewicz Juszkiewicz NNP 10_1101-2020_11_24_390039 204 7 , , , 10_1101-2020_11_24_390039 204 8 S. S. NNP 10_1101-2020_11_24_390039 204 9 , , , 10_1101-2020_11_24_390039 204 10 Guna Guna NNP 10_1101-2020_11_24_390039 204 11 , , , 10_1101-2020_11_24_390039 204 12 A. a. NN 10_1101-2020_11_24_390039 204 13 , , , 10_1101-2020_11_24_390039 204 14 Shao Shao NNP 10_1101-2020_11_24_390039 204 15 , , , 10_1101-2020_11_24_390039 204 16 S. S. NNP 10_1101-2020_11_24_390039 204 17 , , , 10_1101-2020_11_24_390039 204 18 and and CC 10_1101-2020_11_24_390039 204 19 Hegde Hegde NNP 10_1101-2020_11_24_390039 204 20 , , , 10_1101-2020_11_24_390039 204 21 R. R. NNP 10_1101-2020_11_24_390039 204 22 S. S. NNP 10_1101-2020_11_24_390039 204 23 ( ( -LRB- 10_1101-2020_11_24_390039 204 24 2018 2018 CD 10_1101-2020_11_24_390039 204 25 ) ) -RRB- 10_1101-2020_11_24_390039 204 26 . . . 10_1101-2020_11_24_390039 205 1 EMC EMC NNP 10_1101-2020_11_24_390039 205 2 is be VBZ 10_1101-2020_11_24_390039 205 3 required require VBN 10_1101-2020_11_24_390039 205 4 to to TO 10_1101-2020_11_24_390039 205 5 initiate initiate VB 10_1101-2020_11_24_390039 205 6 accurate accurate JJ 10_1101-2020_11_24_390039 205 7 membrane membrane NN 10_1101-2020_11_24_390039 205 8 protein protein NN 10_1101-2020_11_24_390039 205 9 topogenesis topogenesis NN 10_1101-2020_11_24_390039 205 10 . . . 10_1101-2020_11_24_390039 206 1 Cell cell NN 10_1101-2020_11_24_390039 206 2 . . . 10_1101-2020_11_24_390039 207 1 175 175 CD 10_1101-2020_11_24_390039 207 2 , , , 10_1101-2020_11_24_390039 207 3 1- 1- CD 10_1101-2020_11_24_390039 207 4 13 13 CD 10_1101-2020_11_24_390039 207 5 . . . 10_1101-2020_11_24_390039 208 1 Daly Daly NNP 10_1101-2020_11_24_390039 208 2 , , , 10_1101-2020_11_24_390039 208 3 J. J. NNP 10_1101-2020_11_24_390039 208 4 L. L. NNP 10_1101-2020_11_24_390039 208 5 , , , 10_1101-2020_11_24_390039 208 6 Simonetti Simonetti NNP 10_1101-2020_11_24_390039 208 7 , , , 10_1101-2020_11_24_390039 208 8 B. B. NNP 10_1101-2020_11_24_390039 208 9 , , , 10_1101-2020_11_24_390039 208 10 Klein Klein NNP 10_1101-2020_11_24_390039 208 11 , , , 10_1101-2020_11_24_390039 208 12 K. K. NNP 10_1101-2020_11_24_390039 208 13 , , , 10_1101-2020_11_24_390039 208 14 Chen Chen NNP 10_1101-2020_11_24_390039 208 15 , , , 10_1101-2020_11_24_390039 208 16 K.-E. K.-E. NNP 10_1101-2020_11_24_390039 208 17 , , , 10_1101-2020_11_24_390039 208 18 Kavanagh Kavanagh NNP 10_1101-2020_11_24_390039 208 19 Williamson Williamson NNP 10_1101-2020_11_24_390039 208 20 , , , 10_1101-2020_11_24_390039 208 21 M. M. NNP 10_1101-2020_11_24_390039 208 22 , , , 10_1101-2020_11_24_390039 208 23 Antón Antón NNP 10_1101-2020_11_24_390039 208 24 - - HYPH 10_1101-2020_11_24_390039 208 25 Plágaro Plágaro NNP 10_1101-2020_11_24_390039 208 26 , , , 10_1101-2020_11_24_390039 208 27 C. C. NNP 10_1101-2020_11_24_390039 208 28 , , , 10_1101-2020_11_24_390039 208 29 Shoemark Shoemark NNP 10_1101-2020_11_24_390039 208 30 , , , 10_1101-2020_11_24_390039 208 31 D. D. NNP 10_1101-2020_11_24_390039 208 32 K. K. NNP 10_1101-2020_11_24_390039 208 33 , , , 10_1101-2020_11_24_390039 208 34 Simón Simón NNP 10_1101-2020_11_24_390039 208 35 - - HYPH 10_1101-2020_11_24_390039 208 36 Gracia Gracia NNP 10_1101-2020_11_24_390039 208 37 , , , 10_1101-2020_11_24_390039 208 38 L. L. NNP 10_1101-2020_11_24_390039 208 39 , , , 10_1101-2020_11_24_390039 208 40 Bauer Bauer NNP 10_1101-2020_11_24_390039 208 41 , , , 10_1101-2020_11_24_390039 208 42 M. M. NNP 10_1101-2020_11_24_390039 208 43 et et NNP 10_1101-2020_11_24_390039 208 44 al al NNP 10_1101-2020_11_24_390039 208 45 . . . 10_1101-2020_11_24_390039 209 1 ( ( -LRB- 10_1101-2020_11_24_390039 209 2 2020 2020 CD 10_1101-2020_11_24_390039 209 3 ) ) -RRB- 10_1101-2020_11_24_390039 209 4 . . . 10_1101-2020_11_24_390039 210 1 Neuropilin-1 Neuropilin-1 NNP 10_1101-2020_11_24_390039 210 2 is be VBZ 10_1101-2020_11_24_390039 210 3 a a DT 10_1101-2020_11_24_390039 210 4 host host NN 10_1101-2020_11_24_390039 210 5 factor factor NN 10_1101-2020_11_24_390039 210 6 for for IN 10_1101-2020_11_24_390039 210 7 SARS SARS NNP 10_1101-2020_11_24_390039 210 8 - - HYPH 10_1101-2020_11_24_390039 210 9 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 210 10 infection infection NN 10_1101-2020_11_24_390039 210 11 . . . 10_1101-2020_11_24_390039 211 1 Science science NN 10_1101-2020_11_24_390039 211 2 . . . 10_1101-2020_11_24_390039 212 1 370 370 CD 10_1101-2020_11_24_390039 212 2 , , , 10_1101-2020_11_24_390039 212 3 861- 861- CD 10_1101-2020_11_24_390039 212 4 865 865 CD 10_1101-2020_11_24_390039 212 5 . . . 10_1101-2020_11_24_390039 213 1 Drew Drew NNP 10_1101-2020_11_24_390039 213 2 , , , 10_1101-2020_11_24_390039 213 3 E. E. NNP 10_1101-2020_11_24_390039 213 4 D. D. NNP 10_1101-2020_11_24_390039 213 5 , , , 10_1101-2020_11_24_390039 213 6 and and CC 10_1101-2020_11_24_390039 213 7 Janes Janes NNP 10_1101-2020_11_24_390039 213 8 , , , 10_1101-2020_11_24_390039 213 9 R. R. NNP 10_1101-2020_11_24_390039 213 10 W. W. NNP 10_1101-2020_11_24_390039 213 11 ( ( -LRB- 10_1101-2020_11_24_390039 213 12 2020 2020 CD 10_1101-2020_11_24_390039 213 13 ) ) -RRB- 10_1101-2020_11_24_390039 213 14 . . . 10_1101-2020_11_24_390039 214 1 Identification identification NN 10_1101-2020_11_24_390039 214 2 of of IN 10_1101-2020_11_24_390039 214 3 a a DT 10_1101-2020_11_24_390039 214 4 druggable druggable JJ 10_1101-2020_11_24_390039 214 5 binding bind VBG 10_1101-2020_11_24_390039 214 6 pocket pocket NN 10_1101-2020_11_24_390039 214 7 in in IN 10_1101-2020_11_24_390039 214 8 the the DT 10_1101-2020_11_24_390039 214 9 spike spike JJ 10_1101-2020_11_24_390039 214 10 protein protein NN 10_1101-2020_11_24_390039 214 11 reveals reveal VBZ 10_1101-2020_11_24_390039 214 12 a a DT 10_1101-2020_11_24_390039 214 13 key key JJ 10_1101-2020_11_24_390039 214 14 site site NN 10_1101-2020_11_24_390039 214 15 for for IN 10_1101-2020_11_24_390039 214 16 existing exist VBG 10_1101-2020_11_24_390039 214 17 drugs drug NNS 10_1101-2020_11_24_390039 214 18 potentially potentially RB 10_1101-2020_11_24_390039 214 19 capable capable JJ 10_1101-2020_11_24_390039 214 20 of of IN 10_1101-2020_11_24_390039 214 21 combating combat VBG 10_1101-2020_11_24_390039 214 22 Covid-19 Covid-19 NNP 10_1101-2020_11_24_390039 214 23 infectivity infectivity NN 10_1101-2020_11_24_390039 214 24 . . . 10_1101-2020_11_24_390039 215 1 BMC BMC NNP 10_1101-2020_11_24_390039 215 2 Mol Mol NNP 10_1101-2020_11_24_390039 215 3 . . . 10_1101-2020_11_24_390039 216 1 Cell Cell NNP 10_1101-2020_11_24_390039 216 2 Biol Biol NNP 10_1101-2020_11_24_390039 216 3 . . . 10_1101-2020_11_24_390039 217 1 21 21 CD 10_1101-2020_11_24_390039 217 2 , , , 10_1101-2020_11_24_390039 217 3 49 49 CD 10_1101-2020_11_24_390039 217 4 . . . 10_1101-2020_11_24_390039 218 1 Duart Duart NNP 10_1101-2020_11_24_390039 218 2 , , , 10_1101-2020_11_24_390039 218 3 G. G. NNP 10_1101-2020_11_24_390039 218 4 , , , 10_1101-2020_11_24_390039 218 5 García García NNP 10_1101-2020_11_24_390039 218 6 - - HYPH 10_1101-2020_11_24_390039 218 7 Murria Murria NNP 10_1101-2020_11_24_390039 218 8 , , , 10_1101-2020_11_24_390039 218 9 M. M. NNP 10_1101-2020_11_24_390039 218 10 J. J. NNP 10_1101-2020_11_24_390039 218 11 , , , 10_1101-2020_11_24_390039 218 12 Grau Grau NNP 10_1101-2020_11_24_390039 218 13 , , , 10_1101-2020_11_24_390039 218 14 B. B. NNP 10_1101-2020_11_24_390039 218 15 , , , 10_1101-2020_11_24_390039 218 16 Acosta Acosta NNP 10_1101-2020_11_24_390039 218 17 - - HYPH 10_1101-2020_11_24_390039 218 18 Cáceres Cáceres NNP 10_1101-2020_11_24_390039 218 19 , , , 10_1101-2020_11_24_390039 218 20 J. J. NNP 10_1101-2020_11_24_390039 218 21 M. M. NNP 10_1101-2020_11_24_390039 218 22 , , , 10_1101-2020_11_24_390039 218 23 Martínez- Martínez- NNP 10_1101-2020_11_24_390039 218 24 Gil Gil NNP 10_1101-2020_11_24_390039 218 25 , , , 10_1101-2020_11_24_390039 218 26 L. L. NNP 10_1101-2020_11_24_390039 218 27 , , , 10_1101-2020_11_24_390039 218 28 and and CC 10_1101-2020_11_24_390039 218 29 Mingarro Mingarro NNP 10_1101-2020_11_24_390039 218 30 I. I. NNP 10_1101-2020_11_24_390039 219 1 ( ( -LRB- 10_1101-2020_11_24_390039 219 2 2020 2020 CD 10_1101-2020_11_24_390039 219 3 ) ) -RRB- 10_1101-2020_11_24_390039 219 4 SARS SARS NNP 10_1101-2020_11_24_390039 219 5 - - HYPH 10_1101-2020_11_24_390039 219 6 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 219 7 envelope envelope NNP 10_1101-2020_11_24_390039 219 8 protein protein NN 10_1101-2020_11_24_390039 219 9 topology topology NN 10_1101-2020_11_24_390039 219 10 in in IN 10_1101-2020_11_24_390039 219 11 eukaryotic eukaryotic JJ 10_1101-2020_11_24_390039 219 12 membranes membrane NNS 10_1101-2020_11_24_390039 219 13 . . . 10_1101-2020_11_24_390039 220 1 Open open JJ 10_1101-2020_11_24_390039 220 2 Biol Biol NNP 10_1101-2020_11_24_390039 220 3 . . . 10_1101-2020_11_24_390039 221 1 10 10 CD 10_1101-2020_11_24_390039 221 2 , , , 10_1101-2020_11_24_390039 221 3 200209 200209 CD 10_1101-2020_11_24_390039 221 4 . . . 10_1101-2020_11_24_390039 222 1 Firth firth NN 10_1101-2020_11_24_390039 222 2 , , , 10_1101-2020_11_24_390039 222 3 A. a. NN 10_1101-2020_11_24_390039 222 4 E. E. NNP 10_1101-2020_11_24_390039 222 5 ( ( -LRB- 10_1101-2020_11_24_390039 222 6 2020 2020 CD 10_1101-2020_11_24_390039 222 7 ) ) -RRB- 10_1101-2020_11_24_390039 222 8 . . . 10_1101-2020_11_24_390039 223 1 A a DT 10_1101-2020_11_24_390039 223 2 putative putative JJ 10_1101-2020_11_24_390039 223 3 new new JJ 10_1101-2020_11_24_390039 223 4 SARS SARS NNP 10_1101-2020_11_24_390039 223 5 - - HYPH 10_1101-2020_11_24_390039 223 6 CoV CoV NNP 10_1101-2020_11_24_390039 223 7 protein protein NN 10_1101-2020_11_24_390039 223 8 , , , 10_1101-2020_11_24_390039 223 9 3c 3c CD 10_1101-2020_11_24_390039 223 10 , , , 10_1101-2020_11_24_390039 223 11 encoded encode VBD 10_1101-2020_11_24_390039 223 12 in in IN 10_1101-2020_11_24_390039 223 13 an an DT 10_1101-2020_11_24_390039 223 14 ORF ORF NNP 10_1101-2020_11_24_390039 223 15 overlapping overlap VBG 10_1101-2020_11_24_390039 223 16 ORF3a orf3a NN 10_1101-2020_11_24_390039 223 17 . . . 10_1101-2020_11_24_390039 224 1 J. J. NNP 10_1101-2020_11_24_390039 224 2 Gen. Gen. NNP 10_1101-2020_11_24_390039 224 3 Virol Virol NNP 10_1101-2020_11_24_390039 224 4 . . . 10_1101-2020_11_24_390039 225 1 101 101 CD 10_1101-2020_11_24_390039 225 2 , , , 10_1101-2020_11_24_390039 225 3 1085 1085 CD 10_1101-2020_11_24_390039 225 4 - - SYM 10_1101-2020_11_24_390039 225 5 1089 1089 CD 10_1101-2020_11_24_390039 225 6 . . . 10_1101-2020_11_24_390039 226 1 .CC .CC NFP 10_1101-2020_11_24_390039 226 2 - - : 10_1101-2020_11_24_390039 226 3 BY by IN 10_1101-2020_11_24_390039 226 4 - - HYPH 10_1101-2020_11_24_390039 226 5 ND ND NNP 10_1101-2020_11_24_390039 226 6 4.0 4.0 CD 10_1101-2020_11_24_390039 226 7 International international JJ 10_1101-2020_11_24_390039 226 8 licensemade licensemade NN 10_1101-2020_11_24_390039 226 9 available available JJ 10_1101-2020_11_24_390039 226 10 under under IN 10_1101-2020_11_24_390039 226 11 a a DT 10_1101-2020_11_24_390039 226 12 ( ( -LRB- 10_1101-2020_11_24_390039 226 13 which which WDT 10_1101-2020_11_24_390039 226 14 was be VBD 10_1101-2020_11_24_390039 226 15 not not RB 10_1101-2020_11_24_390039 226 16 certified certify VBN 10_1101-2020_11_24_390039 226 17 by by IN 10_1101-2020_11_24_390039 226 18 peer peer NN 10_1101-2020_11_24_390039 226 19 review review NN 10_1101-2020_11_24_390039 226 20 ) ) -RRB- 10_1101-2020_11_24_390039 226 21 is be VBZ 10_1101-2020_11_24_390039 226 22 the the DT 10_1101-2020_11_24_390039 226 23 author author NN 10_1101-2020_11_24_390039 226 24 / / SYM 10_1101-2020_11_24_390039 226 25 funder funder NN 10_1101-2020_11_24_390039 226 26 , , , 10_1101-2020_11_24_390039 226 27 who who WP 10_1101-2020_11_24_390039 226 28 has have VBZ 10_1101-2020_11_24_390039 226 29 granted grant VBN 10_1101-2020_11_24_390039 226 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 226 31 a a DT 10_1101-2020_11_24_390039 226 32 license license NN 10_1101-2020_11_24_390039 226 33 to to TO 10_1101-2020_11_24_390039 226 34 display display VB 10_1101-2020_11_24_390039 226 35 the the DT 10_1101-2020_11_24_390039 226 36 preprint preprint NN 10_1101-2020_11_24_390039 226 37 in in IN 10_1101-2020_11_24_390039 226 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 226 39 . . . 10_1101-2020_11_24_390039 227 1 It -PRON- PRP 10_1101-2020_11_24_390039 227 2 is be VBZ 10_1101-2020_11_24_390039 227 3 The the DT 10_1101-2020_11_24_390039 227 4 copyright copyright NN 10_1101-2020_11_24_390039 227 5 holder holder NN 10_1101-2020_11_24_390039 227 6 for for IN 10_1101-2020_11_24_390039 227 7 this this DT 10_1101-2020_11_24_390039 227 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 227 9 version version NN 10_1101-2020_11_24_390039 227 10 posted post VBD 10_1101-2020_11_24_390039 227 11 January January NNP 10_1101-2020_11_24_390039 227 12 5 5 CD 10_1101-2020_11_24_390039 227 13 , , , 10_1101-2020_11_24_390039 227 14 2021 2021 CD 10_1101-2020_11_24_390039 227 15 . . . 10_1101-2020_11_24_390039 227 16 ; ; : 10_1101-2020_11_24_390039 227 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 227 18 : : : 10_1101-2020_11_24_390039 227 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 227 20 preprint preprint JJ 10_1101-2020_11_24_390039 227 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 227 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 227 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 227 24 - - HYPH 10_1101-2020_11_24_390039 227 25 F F NNP 10_1101-2020_11_24_390039 227 26 as as IN 10_1101-2020_11_24_390039 227 27 a a DT 10_1101-2020_11_24_390039 227 28 potential potential JJ 10_1101-2020_11_24_390039 227 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 227 30 agent agent NN 10_1101-2020_11_24_390039 227 31 Page Page NNP 10_1101-2020_11_24_390039 227 32 16 16 CD 10_1101-2020_11_24_390039 227 33 of of IN 10_1101-2020_11_24_390039 227 34 21 21 CD 10_1101-2020_11_24_390039 227 35 Gérard Gérard NNP 10_1101-2020_11_24_390039 227 36 , , , 10_1101-2020_11_24_390039 227 37 S. S. NNP 10_1101-2020_11_24_390039 227 38 F. F. NNP 10_1101-2020_11_24_390039 227 39 , , , 10_1101-2020_11_24_390039 227 40 Hall Hall NNP 10_1101-2020_11_24_390039 227 41 , , , 10_1101-2020_11_24_390039 227 42 B. B. NNP 10_1101-2020_11_24_390039 227 43 S. S. NNP 10_1101-2020_11_24_390039 227 44 , , , 10_1101-2020_11_24_390039 227 45 Zaki Zaki NNP 10_1101-2020_11_24_390039 227 46 , , , 10_1101-2020_11_24_390039 227 47 A. a. NN 10_1101-2020_11_24_390039 227 48 M. M. NNP 10_1101-2020_11_24_390039 227 49 , , , 10_1101-2020_11_24_390039 227 50 Corfield Corfield NNP 10_1101-2020_11_24_390039 227 51 , , , 10_1101-2020_11_24_390039 227 52 K. K. NNP 10_1101-2020_11_24_390039 227 53 A. A. NNP 10_1101-2020_11_24_390039 227 54 , , , 10_1101-2020_11_24_390039 227 55 Mayerhofer Mayerhofer NNP 10_1101-2020_11_24_390039 227 56 , , , 10_1101-2020_11_24_390039 227 57 P. P. NNP 10_1101-2020_11_24_390039 227 58 U. U. NNP 10_1101-2020_11_24_390039 227 59 , , , 10_1101-2020_11_24_390039 227 60 Costa Costa NNP 10_1101-2020_11_24_390039 227 61 , , , 10_1101-2020_11_24_390039 227 62 C. C. NNP 10_1101-2020_11_24_390039 227 63 , , , 10_1101-2020_11_24_390039 227 64 Whelligan Whelligan NNP 10_1101-2020_11_24_390039 227 65 , , , 10_1101-2020_11_24_390039 227 66 D. D. NNP 10_1101-2020_11_24_390039 227 67 K. K. NNP 10_1101-2020_11_24_390039 227 68 , , , 10_1101-2020_11_24_390039 227 69 Biggin Biggin NNP 10_1101-2020_11_24_390039 227 70 P. P. NNP 10_1101-2020_11_24_390039 227 71 C. C. NNP 10_1101-2020_11_24_390039 227 72 , , , 10_1101-2020_11_24_390039 227 73 Simmonds Simmonds NNP 10_1101-2020_11_24_390039 227 74 , , , 10_1101-2020_11_24_390039 227 75 R. R. NNP 10_1101-2020_11_24_390039 227 76 E. E. NNP 10_1101-2020_11_24_390039 227 77 , , , 10_1101-2020_11_24_390039 227 78 and and CC 10_1101-2020_11_24_390039 227 79 Higgins Higgins NNP 10_1101-2020_11_24_390039 227 80 , , , 10_1101-2020_11_24_390039 227 81 M. M. NNP 10_1101-2020_11_24_390039 227 82 K. K. NNP 10_1101-2020_11_24_390039 227 83 ( ( -LRB- 10_1101-2020_11_24_390039 227 84 2020 2020 CD 10_1101-2020_11_24_390039 227 85 ) ) -RRB- 10_1101-2020_11_24_390039 227 86 . . . 10_1101-2020_11_24_390039 228 1 Structure structure NN 10_1101-2020_11_24_390039 228 2 of of IN 10_1101-2020_11_24_390039 228 3 the the DT 10_1101-2020_11_24_390039 228 4 inhibited inhibited JJ 10_1101-2020_11_24_390039 228 5 state state NN 10_1101-2020_11_24_390039 228 6 of of IN 10_1101-2020_11_24_390039 228 7 the the DT 10_1101-2020_11_24_390039 228 8 Sec Sec NNP 10_1101-2020_11_24_390039 228 9 translocon translocon NN 10_1101-2020_11_24_390039 228 10 . . . 10_1101-2020_11_24_390039 229 1 Mol Mol NNP 10_1101-2020_11_24_390039 229 2 . . . 10_1101-2020_11_24_390039 230 1 Cell cell NN 10_1101-2020_11_24_390039 230 2 . . . 10_1101-2020_11_24_390039 231 1 79 79 CD 10_1101-2020_11_24_390039 231 2 , , , 10_1101-2020_11_24_390039 231 3 406- 406- CD 10_1101-2020_11_24_390039 231 4 415.e7 415.e7 CD 10_1101-2020_11_24_390039 231 5 . . . 10_1101-2020_11_24_390039 232 1 Gordon Gordon NNP 10_1101-2020_11_24_390039 232 2 , , , 10_1101-2020_11_24_390039 232 3 D. D. NNP 10_1101-2020_11_24_390039 232 4 E. E. NNP 10_1101-2020_11_24_390039 232 5 , , , 10_1101-2020_11_24_390039 232 6 Jang Jang NNP 10_1101-2020_11_24_390039 232 7 , , , 10_1101-2020_11_24_390039 232 8 G. G. NNP 10_1101-2020_11_24_390039 232 9 M. M. NNP 10_1101-2020_11_24_390039 232 10 , , , 10_1101-2020_11_24_390039 232 11 Bouhaddou Bouhaddou NNP 10_1101-2020_11_24_390039 232 12 , , , 10_1101-2020_11_24_390039 232 13 M. M. NNP 10_1101-2020_11_24_390039 232 14 , , , 10_1101-2020_11_24_390039 232 15 Xu Xu NNP 10_1101-2020_11_24_390039 232 16 , , , 10_1101-2020_11_24_390039 232 17 J. J. NNP 10_1101-2020_11_24_390039 232 18 , , , 10_1101-2020_11_24_390039 232 19 Obernier Obernier NNP 10_1101-2020_11_24_390039 232 20 , , , 10_1101-2020_11_24_390039 232 21 K. K. NNP 10_1101-2020_11_24_390039 232 22 , , , 10_1101-2020_11_24_390039 232 23 White White NNP 10_1101-2020_11_24_390039 232 24 , , , 10_1101-2020_11_24_390039 232 25 K. K. NNP 10_1101-2020_11_24_390039 232 26 M. M. NNP 10_1101-2020_11_24_390039 232 27 , , , 10_1101-2020_11_24_390039 232 28 O’Meara O’Meara NNP 10_1101-2020_11_24_390039 232 29 , , , 10_1101-2020_11_24_390039 232 30 M. M. NNP 10_1101-2020_11_24_390039 232 31 J. J. NNP 10_1101-2020_11_24_390039 232 32 , , , 10_1101-2020_11_24_390039 232 33 Rezelj Rezelj NNP 10_1101-2020_11_24_390039 232 34 , , , 10_1101-2020_11_24_390039 232 35 V. V. NNP 10_1101-2020_11_24_390039 232 36 V. V. NNP 10_1101-2020_11_24_390039 232 37 , , , 10_1101-2020_11_24_390039 232 38 Guo Guo NNP 10_1101-2020_11_24_390039 232 39 , , , 10_1101-2020_11_24_390039 232 40 J. J. NNP 10_1101-2020_11_24_390039 233 1 Z. Z. NNP 10_1101-2020_11_24_390039 233 2 , , , 10_1101-2020_11_24_390039 233 3 Swaney Swaney NNP 10_1101-2020_11_24_390039 233 4 , , , 10_1101-2020_11_24_390039 233 5 D. D. NNP 10_1101-2020_11_24_390039 233 6 L. L. NNP 10_1101-2020_11_24_390039 233 7 , , , 10_1101-2020_11_24_390039 233 8 Tummino Tummino NNP 10_1101-2020_11_24_390039 233 9 , , , 10_1101-2020_11_24_390039 233 10 T. T. NNP 10_1101-2020_11_24_390039 233 11 A. A. NNP 10_1101-2020_11_24_390039 233 12 et et FW 10_1101-2020_11_24_390039 233 13 al al NNP 10_1101-2020_11_24_390039 233 14 . . . 10_1101-2020_11_24_390039 234 1 ( ( -LRB- 10_1101-2020_11_24_390039 234 2 2020 2020 CD 10_1101-2020_11_24_390039 234 3 ) ) -RRB- 10_1101-2020_11_24_390039 234 4 A a DT 10_1101-2020_11_24_390039 234 5 SARS SARS NNP 10_1101-2020_11_24_390039 234 6 - - HYPH 10_1101-2020_11_24_390039 234 7 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 234 8 protein protein NNP 10_1101-2020_11_24_390039 234 9 interaction interaction NNP 10_1101-2020_11_24_390039 234 10 map map NNP 10_1101-2020_11_24_390039 234 11 reveals reveal VBZ 10_1101-2020_11_24_390039 234 12 targets target NNS 10_1101-2020_11_24_390039 234 13 for for IN 10_1101-2020_11_24_390039 234 14 drug drug NN 10_1101-2020_11_24_390039 234 15 repurposing repurposing NN 10_1101-2020_11_24_390039 234 16 . . . 10_1101-2020_11_24_390039 235 1 Nature nature NN 10_1101-2020_11_24_390039 235 2 . . . 10_1101-2020_11_24_390039 236 1 583 583 CD 10_1101-2020_11_24_390039 236 2 , , , 10_1101-2020_11_24_390039 236 3 459 459 CD 10_1101-2020_11_24_390039 236 4 - - SYM 10_1101-2020_11_24_390039 236 5 468 468 CD 10_1101-2020_11_24_390039 236 6 . . . 10_1101-2020_11_24_390039 237 1 Grob Grob NNP 10_1101-2020_11_24_390039 237 2 , , , 10_1101-2020_11_24_390039 237 3 S. S. NNP 10_1101-2020_11_24_390039 237 4 , , , 10_1101-2020_11_24_390039 237 5 Jahn Jahn NNP 10_1101-2020_11_24_390039 237 6 , , , 10_1101-2020_11_24_390039 237 7 C. C. NNP 10_1101-2020_11_24_390039 237 8 , , , 10_1101-2020_11_24_390039 237 9 Cushman Cushman NNP 10_1101-2020_11_24_390039 237 10 , , , 10_1101-2020_11_24_390039 237 11 S. S. NNP 10_1101-2020_11_24_390039 237 12 , , , 10_1101-2020_11_24_390039 237 13 Bär Bär NNP 10_1101-2020_11_24_390039 237 14 , , , 10_1101-2020_11_24_390039 237 15 C. C. NNP 10_1101-2020_11_24_390039 237 16 , , , 10_1101-2020_11_24_390039 237 17 and and CC 10_1101-2020_11_24_390039 237 18 Thum Thum NNP 10_1101-2020_11_24_390039 237 19 , , , 10_1101-2020_11_24_390039 237 20 T. T. NNP 10_1101-2020_11_24_390039 237 21 ( ( -LRB- 10_1101-2020_11_24_390039 237 22 2020 2020 CD 10_1101-2020_11_24_390039 237 23 ) ) -RRB- 10_1101-2020_11_24_390039 237 24 . . . 10_1101-2020_11_24_390039 238 1 SARS SARS NNP 10_1101-2020_11_24_390039 238 2 - - HYPH 10_1101-2020_11_24_390039 238 3 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 238 4 receptor receptor NN 10_1101-2020_11_24_390039 238 5 ACE2-dependent ACE2-dependent NNP 10_1101-2020_11_24_390039 238 6 implications implication NNS 10_1101-2020_11_24_390039 238 7 on on IN 10_1101-2020_11_24_390039 238 8 the the DT 10_1101-2020_11_24_390039 238 9 cardiovascular cardiovascular JJ 10_1101-2020_11_24_390039 238 10 system system NN 10_1101-2020_11_24_390039 238 11 : : : 10_1101-2020_11_24_390039 238 12 from from IN 10_1101-2020_11_24_390039 238 13 basic basic JJ 10_1101-2020_11_24_390039 238 14 science science NN 10_1101-2020_11_24_390039 238 15 to to IN 10_1101-2020_11_24_390039 238 16 clinical clinical JJ 10_1101-2020_11_24_390039 238 17 implications implication NNS 10_1101-2020_11_24_390039 238 18 . . . 10_1101-2020_11_24_390039 239 1 J. J. NNP 10_1101-2020_11_24_390039 239 2 Mol Mol NNP 10_1101-2020_11_24_390039 239 3 . . . 10_1101-2020_11_24_390039 240 1 Cell cell NN 10_1101-2020_11_24_390039 240 2 . . . 10_1101-2020_11_24_390039 241 1 Cardiol Cardiol NNP 10_1101-2020_11_24_390039 241 2 . . . 10_1101-2020_11_24_390039 242 1 144 144 CD 10_1101-2020_11_24_390039 242 2 , , , 10_1101-2020_11_24_390039 242 3 47 47 CD 10_1101-2020_11_24_390039 242 4 - - SYM 10_1101-2020_11_24_390039 242 5 53 53 CD 10_1101-2020_11_24_390039 242 6 . . . 10_1101-2020_11_24_390039 243 1 Heaton Heaton NNP 10_1101-2020_11_24_390039 243 2 , , , 10_1101-2020_11_24_390039 243 3 N. N. NNP 10_1101-2020_11_24_390039 243 4 S. S. NNP 10_1101-2020_11_24_390039 243 5 , , , 10_1101-2020_11_24_390039 243 6 Moshkina Moshkina NNP 10_1101-2020_11_24_390039 243 7 , , , 10_1101-2020_11_24_390039 243 8 N. N. NNP 10_1101-2020_11_24_390039 243 9 , , , 10_1101-2020_11_24_390039 243 10 Fenouil Fenouil NNP 10_1101-2020_11_24_390039 243 11 , , , 10_1101-2020_11_24_390039 243 12 R. R. NNP 10_1101-2020_11_24_390039 243 13 , , , 10_1101-2020_11_24_390039 243 14 Gardner Gardner NNP 10_1101-2020_11_24_390039 243 15 , , , 10_1101-2020_11_24_390039 243 16 T. T. NNP 10_1101-2020_11_24_390039 243 17 J. J. NNP 10_1101-2020_11_24_390039 243 18 , , , 10_1101-2020_11_24_390039 243 19 Aguirre Aguirre NNP 10_1101-2020_11_24_390039 243 20 , , , 10_1101-2020_11_24_390039 243 21 S. S. NNP 10_1101-2020_11_24_390039 243 22 , , , 10_1101-2020_11_24_390039 243 23 Shah Shah NNP 10_1101-2020_11_24_390039 243 24 , , , 10_1101-2020_11_24_390039 243 25 P. P. NNP 10_1101-2020_11_24_390039 243 26 S. S. NNP 10_1101-2020_11_24_390039 243 27 , , , 10_1101-2020_11_24_390039 243 28 Zhao Zhao NNP 10_1101-2020_11_24_390039 243 29 , , , 10_1101-2020_11_24_390039 243 30 N. N. NNP 10_1101-2020_11_24_390039 243 31 , , , 10_1101-2020_11_24_390039 243 32 Manganaro Manganaro NNP 10_1101-2020_11_24_390039 243 33 , , , 10_1101-2020_11_24_390039 243 34 L. L. NNP 10_1101-2020_11_24_390039 243 35 , , , 10_1101-2020_11_24_390039 243 36 Hultquist Hultquist NNP 10_1101-2020_11_24_390039 243 37 , , , 10_1101-2020_11_24_390039 243 38 J. J. NNP 10_1101-2020_11_24_390039 243 39 F. F. NNP 10_1101-2020_11_24_390039 243 40 , , , 10_1101-2020_11_24_390039 243 41 Noel Noel NNP 10_1101-2020_11_24_390039 243 42 , , , 10_1101-2020_11_24_390039 243 43 J. J. NNP 10_1101-2020_11_24_390039 243 44 et et NNP 10_1101-2020_11_24_390039 243 45 al al NNP 10_1101-2020_11_24_390039 243 46 . . . 10_1101-2020_11_24_390039 244 1 ( ( -LRB- 10_1101-2020_11_24_390039 244 2 2016 2016 CD 10_1101-2020_11_24_390039 244 3 ) ) -RRB- 10_1101-2020_11_24_390039 244 4 . . . 10_1101-2020_11_24_390039 245 1 Targeting target VBG 10_1101-2020_11_24_390039 245 2 viral viral JJ 10_1101-2020_11_24_390039 245 3 proteostasis proteostasis NN 10_1101-2020_11_24_390039 245 4 limits limit NNS 10_1101-2020_11_24_390039 245 5 influenza influenza NNP 10_1101-2020_11_24_390039 245 6 virus virus NN 10_1101-2020_11_24_390039 245 7 , , , 10_1101-2020_11_24_390039 245 8 HIV HIV NNP 10_1101-2020_11_24_390039 245 9 , , , 10_1101-2020_11_24_390039 245 10 and and CC 10_1101-2020_11_24_390039 245 11 Dengue Dengue NNP 10_1101-2020_11_24_390039 245 12 virus virus NN 10_1101-2020_11_24_390039 245 13 infection infection NN 10_1101-2020_11_24_390039 245 14 . . . 10_1101-2020_11_24_390039 246 1 Immunity immunity NN 10_1101-2020_11_24_390039 246 2 . . . 10_1101-2020_11_24_390039 247 1 44 44 CD 10_1101-2020_11_24_390039 247 2 , , , 10_1101-2020_11_24_390039 247 3 46 46 CD 10_1101-2020_11_24_390039 247 4 - - SYM 10_1101-2020_11_24_390039 247 5 58 58 CD 10_1101-2020_11_24_390039 247 6 . . . 10_1101-2020_11_24_390039 248 1 Li Li NNP 10_1101-2020_11_24_390039 248 2 , , , 10_1101-2020_11_24_390039 248 3 J.-Y. J.-Y. NNP 10_1101-2020_11_24_390039 248 4 , , , 10_1101-2020_11_24_390039 248 5 Liao Liao NNP 10_1101-2020_11_24_390039 248 6 , , , 10_1101-2020_11_24_390039 248 7 C.-H. C.-H. NNP 10_1101-2020_11_24_390039 248 8 , , , 10_1101-2020_11_24_390039 248 9 Wang Wang NNP 10_1101-2020_11_24_390039 248 10 , , , 10_1101-2020_11_24_390039 248 11 Q. Q. NNP 10_1101-2020_11_24_390039 248 12 , , , 10_1101-2020_11_24_390039 248 13 Tan Tan NNP 10_1101-2020_11_24_390039 248 14 , , , 10_1101-2020_11_24_390039 248 15 Y.- Y.- NNP 10_1101-2020_11_24_390039 248 16 . . NNP 10_1101-2020_11_24_390039 248 17 , , , 10_1101-2020_11_24_390039 248 18 Luo Luo NNP 10_1101-2020_11_24_390039 248 19 , , , 10_1101-2020_11_24_390039 248 20 R. R. NNP 10_1101-2020_11_24_390039 248 21 , , , 10_1101-2020_11_24_390039 248 22 Qiu Qiu NNP 10_1101-2020_11_24_390039 248 23 , , , 10_1101-2020_11_24_390039 248 24 Y. Y. NNP 10_1101-2020_11_24_390039 248 25 , , , 10_1101-2020_11_24_390039 248 26 and and CC 10_1101-2020_11_24_390039 248 27 Ge Ge NNP 10_1101-2020_11_24_390039 248 28 , , , 10_1101-2020_11_24_390039 248 29 X.-Y. X.-Y. NNP 10_1101-2020_11_24_390039 249 1 ( ( -LRB- 10_1101-2020_11_24_390039 249 2 2020 2020 CD 10_1101-2020_11_24_390039 249 3 ) ) -RRB- 10_1101-2020_11_24_390039 249 4 . . . 10_1101-2020_11_24_390039 250 1 The the DT 10_1101-2020_11_24_390039 250 2 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 250 3 , , , 10_1101-2020_11_24_390039 250 4 ORF8 ORF8 NNS 10_1101-2020_11_24_390039 250 5 and and CC 10_1101-2020_11_24_390039 250 6 nucleocapsid nucleocapsid JJ 10_1101-2020_11_24_390039 250 7 proteins protein NNS 10_1101-2020_11_24_390039 250 8 of of IN 10_1101-2020_11_24_390039 250 9 SARS SARS NNP 10_1101-2020_11_24_390039 250 10 - - HYPH 10_1101-2020_11_24_390039 250 11 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 250 12 inhibit inhibit NN 10_1101-2020_11_24_390039 250 13 type type NN 10_1101-2020_11_24_390039 250 14 I -PRON- PRP 10_1101-2020_11_24_390039 250 15 interferon interferon VBP 10_1101-2020_11_24_390039 250 16 signalling signal VBG 10_1101-2020_11_24_390039 250 17 pathway pathway NN 10_1101-2020_11_24_390039 250 18 . . . 10_1101-2020_11_24_390039 251 1 Virus Virus NNP 10_1101-2020_11_24_390039 251 2 Res Res NNP 10_1101-2020_11_24_390039 251 3 . . . 10_1101-2020_11_24_390039 252 1 286 286 CD 10_1101-2020_11_24_390039 252 2 , , , 10_1101-2020_11_24_390039 252 3 198074 198074 CD 10_1101-2020_11_24_390039 252 4 . . . 10_1101-2020_11_24_390039 253 1 Luesch Luesch NNP 10_1101-2020_11_24_390039 253 2 , , , 10_1101-2020_11_24_390039 253 3 H. H. NNP 10_1101-2020_11_24_390039 253 4 , , , 10_1101-2020_11_24_390039 253 5 and and CC 10_1101-2020_11_24_390039 253 6 Paavilainen Paavilainen NNP 10_1101-2020_11_24_390039 253 7 , , , 10_1101-2020_11_24_390039 253 8 V. V. NNP 10_1101-2020_11_24_390039 253 9 O. O. NNP 10_1101-2020_11_24_390039 254 1 ( ( -LRB- 10_1101-2020_11_24_390039 254 2 2020 2020 CD 10_1101-2020_11_24_390039 254 3 ) ) -RRB- 10_1101-2020_11_24_390039 254 4 . . . 10_1101-2020_11_24_390039 255 1 Natural natural JJ 10_1101-2020_11_24_390039 255 2 products product NNS 10_1101-2020_11_24_390039 255 3 as as IN 10_1101-2020_11_24_390039 255 4 modulators modulator NNS 10_1101-2020_11_24_390039 255 5 of of IN 10_1101-2020_11_24_390039 255 6 eukaryotic eukaryotic JJ 10_1101-2020_11_24_390039 255 7 protein protein NN 10_1101-2020_11_24_390039 255 8 secretion secretion NN 10_1101-2020_11_24_390039 255 9 . . . 10_1101-2020_11_24_390039 256 1 Nat Nat NNP 10_1101-2020_11_24_390039 256 2 . . . 10_1101-2020_11_24_390039 257 1 Prod Prod NNP 10_1101-2020_11_24_390039 257 2 . . . 10_1101-2020_11_24_390039 258 1 Rep. Rep. NNP 10_1101-2020_11_24_390039 258 2 37 37 CD 10_1101-2020_11_24_390039 258 3 , , , 10_1101-2020_11_24_390039 258 4 717 717 CD 10_1101-2020_11_24_390039 258 5 - - SYM 10_1101-2020_11_24_390039 258 6 736 736 CD 10_1101-2020_11_24_390039 258 7 . . . 10_1101-2020_11_24_390039 259 1 Mast Mast NNP 10_1101-2020_11_24_390039 259 2 , , , 10_1101-2020_11_24_390039 259 3 F. F. NNP 10_1101-2020_11_24_390039 259 4 D. D. NNP 10_1101-2020_11_24_390039 259 5 , , , 10_1101-2020_11_24_390039 259 6 Navare Navare NNP 10_1101-2020_11_24_390039 259 7 , , , 10_1101-2020_11_24_390039 259 8 A. a. NN 10_1101-2020_11_24_390039 259 9 T. T. NNP 10_1101-2020_11_24_390039 259 10 , , , 10_1101-2020_11_24_390039 259 11 van van NNP 10_1101-2020_11_24_390039 259 12 der der IN 10_1101-2020_11_24_390039 259 13 Sloot Sloot NNP 10_1101-2020_11_24_390039 259 14 , , , 10_1101-2020_11_24_390039 259 15 A. a. NN 10_1101-2020_11_24_390039 259 16 M. M. NNP 10_1101-2020_11_24_390039 259 17 , , , 10_1101-2020_11_24_390039 259 18 Coulombe Coulombe NNP 10_1101-2020_11_24_390039 259 19 - - HYPH 10_1101-2020_11_24_390039 259 20 Huntington Huntington NNP 10_1101-2020_11_24_390039 259 21 , , , 10_1101-2020_11_24_390039 259 22 J. J. NNP 10_1101-2020_11_24_390039 259 23 Rout Rout NNP 10_1101-2020_11_24_390039 259 24 , , , 10_1101-2020_11_24_390039 259 25 M. M. NNP 10_1101-2020_11_24_390039 259 26 P. P. NNP 10_1101-2020_11_24_390039 259 27 , , , 10_1101-2020_11_24_390039 259 28 Baliga Baliga NNP 10_1101-2020_11_24_390039 259 29 , , , 10_1101-2020_11_24_390039 259 30 N. N. NNP 10_1101-2020_11_24_390039 259 31 S. S. NNP 10_1101-2020_11_24_390039 259 32 , , , 10_1101-2020_11_24_390039 259 33 Kaushansky Kaushansky NNP 10_1101-2020_11_24_390039 259 34 , , , 10_1101-2020_11_24_390039 259 35 A. A. NNP 10_1101-2020_11_24_390039 259 36 , , , 10_1101-2020_11_24_390039 259 37 Chait Chait NNP 10_1101-2020_11_24_390039 259 38 , , , 10_1101-2020_11_24_390039 259 39 B. B. NNP 10_1101-2020_11_24_390039 259 40 T. T. NNP 10_1101-2020_11_24_390039 259 41 , , , 10_1101-2020_11_24_390039 259 42 Aderem Aderem NNP 10_1101-2020_11_24_390039 259 43 , , , 10_1101-2020_11_24_390039 259 44 A. A. NNP 10_1101-2020_11_24_390039 259 45 , , , 10_1101-2020_11_24_390039 259 46 Rice Rice NNP 10_1101-2020_11_24_390039 259 47 , , , 10_1101-2020_11_24_390039 259 48 C. C. NNP 10_1101-2020_11_24_390039 259 49 M. M. NNP 10_1101-2020_11_24_390039 259 50 et et NNP 10_1101-2020_11_24_390039 259 51 al al NNP 10_1101-2020_11_24_390039 259 52 . . . 10_1101-2020_11_24_390039 260 1 ( ( -LRB- 10_1101-2020_11_24_390039 260 2 2020 2020 CD 10_1101-2020_11_24_390039 260 3 ) ) -RRB- 10_1101-2020_11_24_390039 260 4 . . . 10_1101-2020_11_24_390039 261 1 Crippling crippling JJ 10_1101-2020_11_24_390039 261 2 life life NN 10_1101-2020_11_24_390039 261 3 support support NN 10_1101-2020_11_24_390039 261 4 for for IN 10_1101-2020_11_24_390039 261 5 SARS SARS NNP 10_1101-2020_11_24_390039 261 6 - - HYPH 10_1101-2020_11_24_390039 261 7 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 261 8 and and CC 10_1101-2020_11_24_390039 261 9 other other JJ 10_1101-2020_11_24_390039 261 10 viruses virus NNS 10_1101-2020_11_24_390039 261 11 through through IN 10_1101-2020_11_24_390039 261 12 synthetic synthetic JJ 10_1101-2020_11_24_390039 261 13 lethality lethality NN 10_1101-2020_11_24_390039 261 14 . . . 10_1101-2020_11_24_390039 262 1 J. J. NNP 10_1101-2020_11_24_390039 263 1 Cell cell NN 10_1101-2020_11_24_390039 263 2 . . . 10_1101-2020_11_24_390039 264 1 Biol Biol NNP 10_1101-2020_11_24_390039 264 2 . . . 10_1101-2020_11_24_390039 265 1 219 219 CD 10_1101-2020_11_24_390039 265 2 , , , 10_1101-2020_11_24_390039 265 3 e202006159 e202006159 NN 10_1101-2020_11_24_390039 265 4 . . . 10_1101-2020_11_24_390039 266 1 .CC .CC NFP 10_1101-2020_11_24_390039 266 2 - - : 10_1101-2020_11_24_390039 266 3 BY by IN 10_1101-2020_11_24_390039 266 4 - - HYPH 10_1101-2020_11_24_390039 266 5 ND ND NNP 10_1101-2020_11_24_390039 266 6 4.0 4.0 CD 10_1101-2020_11_24_390039 266 7 International international JJ 10_1101-2020_11_24_390039 266 8 licensemade licensemade NN 10_1101-2020_11_24_390039 266 9 available available JJ 10_1101-2020_11_24_390039 266 10 under under IN 10_1101-2020_11_24_390039 266 11 a a DT 10_1101-2020_11_24_390039 266 12 ( ( -LRB- 10_1101-2020_11_24_390039 266 13 which which WDT 10_1101-2020_11_24_390039 266 14 was be VBD 10_1101-2020_11_24_390039 266 15 not not RB 10_1101-2020_11_24_390039 266 16 certified certify VBN 10_1101-2020_11_24_390039 266 17 by by IN 10_1101-2020_11_24_390039 266 18 peer peer NN 10_1101-2020_11_24_390039 266 19 review review NN 10_1101-2020_11_24_390039 266 20 ) ) -RRB- 10_1101-2020_11_24_390039 266 21 is be VBZ 10_1101-2020_11_24_390039 266 22 the the DT 10_1101-2020_11_24_390039 266 23 author author NN 10_1101-2020_11_24_390039 266 24 / / SYM 10_1101-2020_11_24_390039 266 25 funder funder NN 10_1101-2020_11_24_390039 266 26 , , , 10_1101-2020_11_24_390039 266 27 who who WP 10_1101-2020_11_24_390039 266 28 has have VBZ 10_1101-2020_11_24_390039 266 29 granted grant VBN 10_1101-2020_11_24_390039 266 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 266 31 a a DT 10_1101-2020_11_24_390039 266 32 license license NN 10_1101-2020_11_24_390039 266 33 to to TO 10_1101-2020_11_24_390039 266 34 display display VB 10_1101-2020_11_24_390039 266 35 the the DT 10_1101-2020_11_24_390039 266 36 preprint preprint NN 10_1101-2020_11_24_390039 266 37 in in IN 10_1101-2020_11_24_390039 266 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 266 39 . . . 10_1101-2020_11_24_390039 267 1 It -PRON- PRP 10_1101-2020_11_24_390039 267 2 is be VBZ 10_1101-2020_11_24_390039 267 3 The the DT 10_1101-2020_11_24_390039 267 4 copyright copyright NN 10_1101-2020_11_24_390039 267 5 holder holder NN 10_1101-2020_11_24_390039 267 6 for for IN 10_1101-2020_11_24_390039 267 7 this this DT 10_1101-2020_11_24_390039 267 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 267 9 version version NN 10_1101-2020_11_24_390039 267 10 posted post VBD 10_1101-2020_11_24_390039 267 11 January January NNP 10_1101-2020_11_24_390039 267 12 5 5 CD 10_1101-2020_11_24_390039 267 13 , , , 10_1101-2020_11_24_390039 267 14 2021 2021 CD 10_1101-2020_11_24_390039 267 15 . . . 10_1101-2020_11_24_390039 267 16 ; ; : 10_1101-2020_11_24_390039 267 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 267 18 : : : 10_1101-2020_11_24_390039 267 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 267 20 preprint preprint JJ 10_1101-2020_11_24_390039 267 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 267 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 267 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 267 24 - - HYPH 10_1101-2020_11_24_390039 267 25 F F NNP 10_1101-2020_11_24_390039 267 26 as as IN 10_1101-2020_11_24_390039 267 27 a a DT 10_1101-2020_11_24_390039 267 28 potential potential JJ 10_1101-2020_11_24_390039 267 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 267 30 agent agent NN 10_1101-2020_11_24_390039 267 31 Page Page NNP 10_1101-2020_11_24_390039 267 32 17 17 CD 10_1101-2020_11_24_390039 267 33 of of IN 10_1101-2020_11_24_390039 267 34 21 21 CD 10_1101-2020_11_24_390039 267 35 McKenna McKenna NNP 10_1101-2020_11_24_390039 267 36 , , , 10_1101-2020_11_24_390039 267 37 M. M. NNP 10_1101-2020_11_24_390039 267 38 , , , 10_1101-2020_11_24_390039 267 39 Simmonds Simmonds NNP 10_1101-2020_11_24_390039 267 40 , , , 10_1101-2020_11_24_390039 267 41 R.E. R.E. NNP 10_1101-2020_11_24_390039 267 42 , , , 10_1101-2020_11_24_390039 267 43 and and CC 10_1101-2020_11_24_390039 267 44 High High NNP 10_1101-2020_11_24_390039 267 45 , , , 10_1101-2020_11_24_390039 267 46 S. S. NNP 10_1101-2020_11_24_390039 267 47 ( ( -LRB- 10_1101-2020_11_24_390039 267 48 2016 2016 CD 10_1101-2020_11_24_390039 267 49 ) ) -RRB- 10_1101-2020_11_24_390039 267 50 . . . 10_1101-2020_11_24_390039 268 1 Mechanistic mechanistic JJ 10_1101-2020_11_24_390039 268 2 insights insight NNS 10_1101-2020_11_24_390039 268 3 into into IN 10_1101-2020_11_24_390039 268 4 the the DT 10_1101-2020_11_24_390039 268 5 inhibition inhibition NN 10_1101-2020_11_24_390039 268 6 of of IN 10_1101-2020_11_24_390039 268 7 Sec61-dependent sec61-dependent NN 10_1101-2020_11_24_390039 268 8 co- co- JJ 10_1101-2020_11_24_390039 268 9 and and CC 10_1101-2020_11_24_390039 268 10 post post JJ 10_1101-2020_11_24_390039 268 11 - - JJ 10_1101-2020_11_24_390039 268 12 translational translational JJ 10_1101-2020_11_24_390039 268 13 translocation translocation NN 10_1101-2020_11_24_390039 268 14 by by IN 10_1101-2020_11_24_390039 268 15 mycolactone mycolactone NN 10_1101-2020_11_24_390039 268 16 . . . 10_1101-2020_11_24_390039 269 1 J. J. NNP 10_1101-2020_11_24_390039 270 1 Cell Cell NNP 10_1101-2020_11_24_390039 270 2 Sci Sci NNP 10_1101-2020_11_24_390039 270 3 . . . 10_1101-2020_11_24_390039 271 1 129 129 CD 10_1101-2020_11_24_390039 271 2 , , , 10_1101-2020_11_24_390039 271 3 1404 1404 CD 10_1101-2020_11_24_390039 271 4 - - SYM 10_1101-2020_11_24_390039 271 5 1415 1415 CD 10_1101-2020_11_24_390039 271 6 . . . 10_1101-2020_11_24_390039 272 1 Morel Morel NNP 10_1101-2020_11_24_390039 272 2 , , , 10_1101-2020_11_24_390039 272 3 J. J. NNP 10_1101-2020_11_24_390039 272 4 D. D. NNP 10_1101-2020_11_24_390039 272 5 , , , 10_1101-2020_11_24_390039 272 6 Paatero Paatero NNP 10_1101-2020_11_24_390039 272 7 , , , 10_1101-2020_11_24_390039 272 8 A. a. NN 10_1101-2020_11_24_390039 272 9 O. O. NNP 10_1101-2020_11_24_390039 272 10 , , , 10_1101-2020_11_24_390039 272 11 Wei Wei NNP 10_1101-2020_11_24_390039 272 12 , , , 10_1101-2020_11_24_390039 272 13 J. J. NNP 10_1101-2020_11_24_390039 272 14 , , , 10_1101-2020_11_24_390039 272 15 Yewdell Yewdell NNP 10_1101-2020_11_24_390039 272 16 , , , 10_1101-2020_11_24_390039 272 17 J. J. NNP 10_1101-2020_11_24_390039 272 18 W. W. NNP 10_1101-2020_11_24_390039 272 19 , , , 10_1101-2020_11_24_390039 272 20 Guenin Guenin NNP 10_1101-2020_11_24_390039 272 21 - - HYPH 10_1101-2020_11_24_390039 272 22 Macé Macé NNP 10_1101-2020_11_24_390039 272 23 , , , 10_1101-2020_11_24_390039 272 24 L. L. NNP 10_1101-2020_11_24_390039 272 25 , , , 10_1101-2020_11_24_390039 272 26 Van Van NNP 10_1101-2020_11_24_390039 272 27 Haver Haver NNP 10_1101-2020_11_24_390039 272 28 , , , 10_1101-2020_11_24_390039 272 29 D. D. NNP 10_1101-2020_11_24_390039 272 30 , , , 10_1101-2020_11_24_390039 272 31 Impens Impens NNP 10_1101-2020_11_24_390039 272 32 , , , 10_1101-2020_11_24_390039 272 33 F. F. NNP 10_1101-2020_11_24_390039 272 34 , , , 10_1101-2020_11_24_390039 272 35 Pietrosemoli Pietrosemoli NNP 10_1101-2020_11_24_390039 272 36 , , , 10_1101-2020_11_24_390039 272 37 N. N. NNP 10_1101-2020_11_24_390039 272 38 , , , 10_1101-2020_11_24_390039 272 39 Paavilainen Paavilainen NNP 10_1101-2020_11_24_390039 272 40 , , , 10_1101-2020_11_24_390039 272 41 V. V. NNP 10_1101-2020_11_24_390039 272 42 O. O. NNP 10_1101-2020_11_24_390039 272 43 , , , 10_1101-2020_11_24_390039 272 44 and and CC 10_1101-2020_11_24_390039 272 45 Demangel Demangel NNP 10_1101-2020_11_24_390039 272 46 , , , 10_1101-2020_11_24_390039 272 47 C. C. NNP 10_1101-2020_11_24_390039 272 48 ( ( -LRB- 10_1101-2020_11_24_390039 272 49 2018 2018 CD 10_1101-2020_11_24_390039 272 50 ) ) -RRB- 10_1101-2020_11_24_390039 272 51 . . . 10_1101-2020_11_24_390039 273 1 Proteomics proteomic NNS 10_1101-2020_11_24_390039 273 2 reveals reveal VBZ 10_1101-2020_11_24_390039 273 3 scope scope NN 10_1101-2020_11_24_390039 273 4 of of IN 10_1101-2020_11_24_390039 273 5 mycolactone mycolactone NN 10_1101-2020_11_24_390039 273 6 - - HYPH 10_1101-2020_11_24_390039 273 7 mediated mediate VBN 10_1101-2020_11_24_390039 273 8 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 273 9 blockade blockade NN 10_1101-2020_11_24_390039 273 10 and and CC 10_1101-2020_11_24_390039 273 11 distinctive distinctive JJ 10_1101-2020_11_24_390039 273 12 stress stress NN 10_1101-2020_11_24_390039 273 13 signature signature NN 10_1101-2020_11_24_390039 273 14 . . . 10_1101-2020_11_24_390039 274 1 Mol Mol NNP 10_1101-2020_11_24_390039 274 2 . . . 10_1101-2020_11_24_390039 275 1 Cell Cell NNP 10_1101-2020_11_24_390039 275 2 Prot Prot NNP 10_1101-2020_11_24_390039 275 3 . . . 10_1101-2020_11_24_390039 276 1 17 17 CD 10_1101-2020_11_24_390039 276 2 , , , 10_1101-2020_11_24_390039 276 3 1750 1750 CD 10_1101-2020_11_24_390039 276 4 - - SYM 10_1101-2020_11_24_390039 276 5 1765 1765 CD 10_1101-2020_11_24_390039 276 6 . . . 10_1101-2020_11_24_390039 277 1 Naqvi Naqvi NNP 10_1101-2020_11_24_390039 277 2 , , , 10_1101-2020_11_24_390039 277 3 A. a. NN 10_1101-2020_11_24_390039 278 1 A. A. NNP 10_1101-2020_11_24_390039 278 2 T. T. NNP 10_1101-2020_11_24_390039 278 3 , , , 10_1101-2020_11_24_390039 278 4 Fatima Fatima NNP 10_1101-2020_11_24_390039 278 5 , , , 10_1101-2020_11_24_390039 278 6 K. K. NNP 10_1101-2020_11_24_390039 278 7 , , , 10_1101-2020_11_24_390039 278 8 Mohammad Mohammad NNP 10_1101-2020_11_24_390039 278 9 , , , 10_1101-2020_11_24_390039 278 10 T. T. NNP 10_1101-2020_11_24_390039 278 11 , , , 10_1101-2020_11_24_390039 278 12 Fatima Fatima NNP 10_1101-2020_11_24_390039 278 13 , , , 10_1101-2020_11_24_390039 278 14 U. U. NNP 10_1101-2020_11_24_390039 278 15 , , , 10_1101-2020_11_24_390039 278 16 Singh Singh NNP 10_1101-2020_11_24_390039 278 17 , , , 10_1101-2020_11_24_390039 278 18 I. I. NNP 10_1101-2020_11_24_390039 278 19 K. K. NNP 10_1101-2020_11_24_390039 278 20 , , , 10_1101-2020_11_24_390039 278 21 Singh Singh NNP 10_1101-2020_11_24_390039 278 22 , , , 10_1101-2020_11_24_390039 278 23 A. a. NN 10_1101-2020_11_24_390039 278 24 , , , 10_1101-2020_11_24_390039 278 25 Atif Atif NNP 10_1101-2020_11_24_390039 278 26 , , , 10_1101-2020_11_24_390039 278 27 A. a. NN 10_1101-2020_11_24_390039 278 28 M. M. NNP 10_1101-2020_11_24_390039 278 29 , , , 10_1101-2020_11_24_390039 278 30 Hariprasad Hariprasad NNP 10_1101-2020_11_24_390039 278 31 , , , 10_1101-2020_11_24_390039 278 32 G. G. NNP 10_1101-2020_11_24_390039 278 33 , , , 10_1101-2020_11_24_390039 278 34 Hasan Hasan NNP 10_1101-2020_11_24_390039 278 35 , , , 10_1101-2020_11_24_390039 278 36 G. G. NNP 10_1101-2020_11_24_390039 278 37 M. M. NNP 10_1101-2020_11_24_390039 278 38 , , , 10_1101-2020_11_24_390039 278 39 and and CC 10_1101-2020_11_24_390039 278 40 Hassan Hassan NNP 10_1101-2020_11_24_390039 278 41 , , , 10_1101-2020_11_24_390039 278 42 M. M. NNP 10_1101-2020_11_24_390039 278 43 I. I. NNP 10_1101-2020_11_24_390039 279 1 ( ( -LRB- 10_1101-2020_11_24_390039 279 2 2020 2020 CD 10_1101-2020_11_24_390039 279 3 ) ) -RRB- 10_1101-2020_11_24_390039 279 4 Insights insight NNS 10_1101-2020_11_24_390039 279 5 into into IN 10_1101-2020_11_24_390039 279 6 SARS SARS NNP 10_1101-2020_11_24_390039 279 7 - - HYPH 10_1101-2020_11_24_390039 279 8 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 279 9 genome genome NN 10_1101-2020_11_24_390039 279 10 , , , 10_1101-2020_11_24_390039 279 11 structure structure NN 10_1101-2020_11_24_390039 279 12 , , , 10_1101-2020_11_24_390039 279 13 evolution evolution NN 10_1101-2020_11_24_390039 279 14 , , , 10_1101-2020_11_24_390039 279 15 pathogenesis pathogenesis NN 10_1101-2020_11_24_390039 279 16 and and CC 10_1101-2020_11_24_390039 279 17 therapies therapy NNS 10_1101-2020_11_24_390039 279 18 : : : 10_1101-2020_11_24_390039 279 19 structural structural JJ 10_1101-2020_11_24_390039 279 20 genomics genomic NNS 10_1101-2020_11_24_390039 279 21 approach approach NN 10_1101-2020_11_24_390039 279 22 . . . 10_1101-2020_11_24_390039 280 1 Biochim Biochim NNP 10_1101-2020_11_24_390039 280 2 . . . 10_1101-2020_11_24_390039 281 1 Biophys Biophys NNP 10_1101-2020_11_24_390039 281 2 . . . 10_1101-2020_11_24_390039 282 1 Acta Acta NNP 10_1101-2020_11_24_390039 282 2 . . . 10_1101-2020_11_24_390039 283 1 Mol Mol NNP 10_1101-2020_11_24_390039 283 2 . . . 10_1101-2020_11_24_390039 284 1 Basis Basis NNP 10_1101-2020_11_24_390039 284 2 Dis Dis NNP 10_1101-2020_11_24_390039 284 3 . . . 10_1101-2020_11_24_390039 285 1 1866 1866 CD 10_1101-2020_11_24_390039 285 2 , , , 10_1101-2020_11_24_390039 285 3 165878 165878 CD 10_1101-2020_11_24_390039 285 4 . . . 10_1101-2020_11_24_390039 286 1 Netland Netland NNP 10_1101-2020_11_24_390039 286 2 , , , 10_1101-2020_11_24_390039 286 3 J. J. NNP 10_1101-2020_11_24_390039 286 4 , , , 10_1101-2020_11_24_390039 286 5 Ferraro Ferraro NNP 10_1101-2020_11_24_390039 286 6 , , , 10_1101-2020_11_24_390039 286 7 D. D. NNP 10_1101-2020_11_24_390039 286 8 , , , 10_1101-2020_11_24_390039 286 9 Pewe Pewe NNP 10_1101-2020_11_24_390039 286 10 , , , 10_1101-2020_11_24_390039 286 11 L. L. NNP 10_1101-2020_11_24_390039 286 12 , , , 10_1101-2020_11_24_390039 286 13 Olivares Olivares NNP 10_1101-2020_11_24_390039 286 14 , , , 10_1101-2020_11_24_390039 286 15 H. H. NNP 10_1101-2020_11_24_390039 286 16 , , , 10_1101-2020_11_24_390039 286 17 Gallagher Gallagher NNP 10_1101-2020_11_24_390039 286 18 , , , 10_1101-2020_11_24_390039 286 19 T. t. NN 10_1101-2020_11_24_390039 286 20 , , , 10_1101-2020_11_24_390039 286 21 and and CC 10_1101-2020_11_24_390039 286 22 Perlman Perlman NNP 10_1101-2020_11_24_390039 286 23 , , , 10_1101-2020_11_24_390039 286 24 S. S. NNP 10_1101-2020_11_24_390039 286 25 ( ( -LRB- 10_1101-2020_11_24_390039 286 26 2007 2007 CD 10_1101-2020_11_24_390039 286 27 ) ) -RRB- 10_1101-2020_11_24_390039 286 28 . . . 10_1101-2020_11_24_390039 287 1 Enhancement enhancement NN 10_1101-2020_11_24_390039 287 2 of of IN 10_1101-2020_11_24_390039 287 3 murine murine NNP 10_1101-2020_11_24_390039 287 4 coronavirus coronavirus NNP 10_1101-2020_11_24_390039 287 5 replication replication NNP 10_1101-2020_11_24_390039 287 6 by by IN 10_1101-2020_11_24_390039 287 7 severe severe JJ 10_1101-2020_11_24_390039 287 8 acute acute JJ 10_1101-2020_11_24_390039 287 9 respiratory respiratory JJ 10_1101-2020_11_24_390039 287 10 syndrome syndrome NN 10_1101-2020_11_24_390039 287 11 coronavirus coronavirus NNP 10_1101-2020_11_24_390039 287 12 protein protein NN 10_1101-2020_11_24_390039 287 13 6 6 CD 10_1101-2020_11_24_390039 287 14 requires require VBZ 10_1101-2020_11_24_390039 287 15 the the DT 10_1101-2020_11_24_390039 287 16 N n JJ 10_1101-2020_11_24_390039 287 17 - - HYPH 10_1101-2020_11_24_390039 287 18 terminal terminal JJ 10_1101-2020_11_24_390039 287 19 hydrophobic hydrophobic NN 10_1101-2020_11_24_390039 287 20 region region NN 10_1101-2020_11_24_390039 287 21 but but CC 10_1101-2020_11_24_390039 287 22 not not RB 10_1101-2020_11_24_390039 287 23 C c NN 10_1101-2020_11_24_390039 287 24 - - HYPH 10_1101-2020_11_24_390039 287 25 terminal terminal JJ 10_1101-2020_11_24_390039 287 26 sorting sort VBG 10_1101-2020_11_24_390039 287 27 motifs motif NNS 10_1101-2020_11_24_390039 287 28 . . . 10_1101-2020_11_24_390039 288 1 J. J. NNP 10_1101-2020_11_24_390039 288 2 Virol Virol NNP 10_1101-2020_11_24_390039 288 3 . . . 10_1101-2020_11_24_390039 289 1 81 81 CD 10_1101-2020_11_24_390039 289 2 , , , 10_1101-2020_11_24_390039 289 3 11520 11520 CD 10_1101-2020_11_24_390039 289 4 - - SYM 10_1101-2020_11_24_390039 289 5 11525 11525 CD 10_1101-2020_11_24_390039 289 6 . . . 10_1101-2020_11_24_390039 290 1 Nilsson Nilsson NNP 10_1101-2020_11_24_390039 290 2 , , , 10_1101-2020_11_24_390039 290 3 I. I. NNP 10_1101-2020_11_24_390039 290 4 M. M. NNP 10_1101-2020_11_24_390039 290 5 , , , 10_1101-2020_11_24_390039 290 6 and and CC 10_1101-2020_11_24_390039 290 7 von von NNP 10_1101-2020_11_24_390039 290 8 Heijne Heijne NNP 10_1101-2020_11_24_390039 290 9 , , , 10_1101-2020_11_24_390039 290 10 G. G. NNP 10_1101-2020_11_24_390039 290 11 ( ( -LRB- 10_1101-2020_11_24_390039 290 12 1993 1993 CD 10_1101-2020_11_24_390039 290 13 ) ) -RRB- 10_1101-2020_11_24_390039 290 14 . . . 10_1101-2020_11_24_390039 291 1 Determination determination NN 10_1101-2020_11_24_390039 291 2 of of IN 10_1101-2020_11_24_390039 291 3 the the DT 10_1101-2020_11_24_390039 291 4 distance distance NN 10_1101-2020_11_24_390039 291 5 between between IN 10_1101-2020_11_24_390039 291 6 the the DT 10_1101-2020_11_24_390039 291 7 oligosaccharyltransferase oligosaccharyltransferase JJ 10_1101-2020_11_24_390039 291 8 active active JJ 10_1101-2020_11_24_390039 291 9 site site NN 10_1101-2020_11_24_390039 291 10 and and CC 10_1101-2020_11_24_390039 291 11 the the DT 10_1101-2020_11_24_390039 291 12 endoplasmic endoplasmic JJ 10_1101-2020_11_24_390039 291 13 reticulum reticulum NN 10_1101-2020_11_24_390039 291 14 membrane membrane NN 10_1101-2020_11_24_390039 291 15 . . . 10_1101-2020_11_24_390039 292 1 J. J. NNP 10_1101-2020_11_24_390039 292 2 Biol Biol NNP 10_1101-2020_11_24_390039 292 3 . . . 10_1101-2020_11_24_390039 293 1 Chem Chem NNP 10_1101-2020_11_24_390039 293 2 . . . 10_1101-2020_11_24_390039 294 1 268 268 CD 10_1101-2020_11_24_390039 294 2 , , , 10_1101-2020_11_24_390039 294 3 5798 5798 CD 10_1101-2020_11_24_390039 294 4 - - SYM 10_1101-2020_11_24_390039 294 5 5801 5801 CD 10_1101-2020_11_24_390039 294 6 . . . 10_1101-2020_11_24_390039 295 1 Ray Ray NNP 10_1101-2020_11_24_390039 295 2 - - HYPH 10_1101-2020_11_24_390039 295 3 Sinha Sinha NNP 10_1101-2020_11_24_390039 295 4 , , , 10_1101-2020_11_24_390039 295 5 A. A. NNP 10_1101-2020_11_24_390039 295 6 , , , 10_1101-2020_11_24_390039 295 7 Cross Cross NNP 10_1101-2020_11_24_390039 295 8 , , , 10_1101-2020_11_24_390039 295 9 B. B. NNP 10_1101-2020_11_24_390039 295 10 C. C. NNP 10_1101-2020_11_24_390039 295 11 S. S. NNP 10_1101-2020_11_24_390039 295 12 , , , 10_1101-2020_11_24_390039 295 13 Mironov Mironov NNP 10_1101-2020_11_24_390039 295 14 , , , 10_1101-2020_11_24_390039 295 15 A. A. NNP 10_1101-2020_11_24_390039 295 16 , , , 10_1101-2020_11_24_390039 295 17 Wiertz Wiertz NNP 10_1101-2020_11_24_390039 295 18 , , , 10_1101-2020_11_24_390039 295 19 E. E. NNP 10_1101-2020_11_24_390039 295 20 , , , 10_1101-2020_11_24_390039 295 21 and and CC 10_1101-2020_11_24_390039 295 22 High High NNP 10_1101-2020_11_24_390039 295 23 , , , 10_1101-2020_11_24_390039 295 24 S. S. NNP 10_1101-2020_11_24_390039 295 25 ( ( -LRB- 10_1101-2020_11_24_390039 295 26 2009 2009 CD 10_1101-2020_11_24_390039 295 27 ) ) -RRB- 10_1101-2020_11_24_390039 295 28 . . . 10_1101-2020_11_24_390039 296 1 Endoplasmic endoplasmic JJ 10_1101-2020_11_24_390039 296 2 reticulum reticulum NN 10_1101-2020_11_24_390039 296 3 - - HYPH 10_1101-2020_11_24_390039 296 4 associated associate VBN 10_1101-2020_11_24_390039 296 5 degradation degradation NN 10_1101-2020_11_24_390039 296 6 of of IN 10_1101-2020_11_24_390039 296 7 a a DT 10_1101-2020_11_24_390039 296 8 degron degron NN 10_1101-2020_11_24_390039 296 9 - - HYPH 10_1101-2020_11_24_390039 296 10 containing contain VBG 10_1101-2020_11_24_390039 296 11 polytopic polytopic JJ 10_1101-2020_11_24_390039 296 12 membrane membrane NN 10_1101-2020_11_24_390039 296 13 protein protein NN 10_1101-2020_11_24_390039 296 14 . . . 10_1101-2020_11_24_390039 297 1 Mol Mol NNP 10_1101-2020_11_24_390039 297 2 . . . 10_1101-2020_11_24_390039 298 1 Membr Membr NNP 10_1101-2020_11_24_390039 298 2 . . . 10_1101-2020_11_24_390039 299 1 Biol Biol NNP 10_1101-2020_11_24_390039 299 2 . . . 10_1101-2020_11_24_390039 300 1 26 26 CD 10_1101-2020_11_24_390039 300 2 , , , 10_1101-2020_11_24_390039 300 3 448 448 CD 10_1101-2020_11_24_390039 300 4 - - SYM 10_1101-2020_11_24_390039 300 5 464 464 CD 10_1101-2020_11_24_390039 300 6 . . . 10_1101-2020_11_24_390039 301 1 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 301 2 , , , 10_1101-2020_11_24_390039 301 3 S. S. NNP 10_1101-2020_11_24_390039 301 4 , , , 10_1101-2020_11_24_390039 301 5 Zong Zong NNP 10_1101-2020_11_24_390039 301 6 , , , 10_1101-2020_11_24_390039 301 7 G. G. NNP 10_1101-2020_11_24_390039 301 8 , , , 10_1101-2020_11_24_390039 301 9 Duah Duah NNP 10_1101-2020_11_24_390039 301 10 , , , 10_1101-2020_11_24_390039 301 11 K. K. NNP 10_1101-2020_11_24_390039 301 12 B. B. NNP 10_1101-2020_11_24_390039 301 13 , , , 10_1101-2020_11_24_390039 301 14 Andrews Andrews NNP 10_1101-2020_11_24_390039 301 15 , , , 10_1101-2020_11_24_390039 301 16 L. L. NNP 10_1101-2020_11_24_390039 301 17 E. E. NNP 10_1101-2020_11_24_390039 301 18 , , , 10_1101-2020_11_24_390039 301 19 Shi Shi NNP 10_1101-2020_11_24_390039 301 20 , , , 10_1101-2020_11_24_390039 301 21 W. W. NNP 10_1101-2020_11_24_390039 301 22 Q. Q. NNP 10_1101-2020_11_24_390039 301 23 , , , 10_1101-2020_11_24_390039 301 24 and and CC 10_1101-2020_11_24_390039 301 25 High High NNP 10_1101-2020_11_24_390039 301 26 , , , 10_1101-2020_11_24_390039 301 27 S. S. NNP 10_1101-2020_11_24_390039 301 28 ( ( -LRB- 10_1101-2020_11_24_390039 301 29 2020 2020 CD 10_1101-2020_11_24_390039 301 30 ) ) -RRB- 10_1101-2020_11_24_390039 301 31 . . . 10_1101-2020_11_24_390039 302 1 Type type NN 10_1101-2020_11_24_390039 302 2 III III NNP 10_1101-2020_11_24_390039 302 3 transmembrane transmembrane JJ 10_1101-2020_11_24_390039 302 4 protein protein NN 10_1101-2020_11_24_390039 302 5 integration integration NN 10_1101-2020_11_24_390039 302 6 requires require VBZ 10_1101-2020_11_24_390039 302 7 both both CC 10_1101-2020_11_24_390039 302 8 the the DT 10_1101-2020_11_24_390039 302 9 EMC EMC NNP 10_1101-2020_11_24_390039 302 10 and and CC 10_1101-2020_11_24_390039 302 11 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 302 12 complex complex JJ 10_1101-2020_11_24_390039 302 13 . . . 10_1101-2020_11_24_390039 303 1 Submitted submit VBN 10_1101-2020_11_24_390039 303 2 . . . 10_1101-2020_11_24_390039 304 1 .CC .CC NFP 10_1101-2020_11_24_390039 304 2 - - : 10_1101-2020_11_24_390039 304 3 BY by IN 10_1101-2020_11_24_390039 304 4 - - HYPH 10_1101-2020_11_24_390039 304 5 ND ND NNP 10_1101-2020_11_24_390039 304 6 4.0 4.0 CD 10_1101-2020_11_24_390039 304 7 International international JJ 10_1101-2020_11_24_390039 304 8 licensemade licensemade NN 10_1101-2020_11_24_390039 304 9 available available JJ 10_1101-2020_11_24_390039 304 10 under under IN 10_1101-2020_11_24_390039 304 11 a a DT 10_1101-2020_11_24_390039 304 12 ( ( -LRB- 10_1101-2020_11_24_390039 304 13 which which WDT 10_1101-2020_11_24_390039 304 14 was be VBD 10_1101-2020_11_24_390039 304 15 not not RB 10_1101-2020_11_24_390039 304 16 certified certify VBN 10_1101-2020_11_24_390039 304 17 by by IN 10_1101-2020_11_24_390039 304 18 peer peer NN 10_1101-2020_11_24_390039 304 19 review review NN 10_1101-2020_11_24_390039 304 20 ) ) -RRB- 10_1101-2020_11_24_390039 304 21 is be VBZ 10_1101-2020_11_24_390039 304 22 the the DT 10_1101-2020_11_24_390039 304 23 author author NN 10_1101-2020_11_24_390039 304 24 / / SYM 10_1101-2020_11_24_390039 304 25 funder funder NN 10_1101-2020_11_24_390039 304 26 , , , 10_1101-2020_11_24_390039 304 27 who who WP 10_1101-2020_11_24_390039 304 28 has have VBZ 10_1101-2020_11_24_390039 304 29 granted grant VBN 10_1101-2020_11_24_390039 304 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 304 31 a a DT 10_1101-2020_11_24_390039 304 32 license license NN 10_1101-2020_11_24_390039 304 33 to to TO 10_1101-2020_11_24_390039 304 34 display display VB 10_1101-2020_11_24_390039 304 35 the the DT 10_1101-2020_11_24_390039 304 36 preprint preprint NN 10_1101-2020_11_24_390039 304 37 in in IN 10_1101-2020_11_24_390039 304 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 304 39 . . . 10_1101-2020_11_24_390039 305 1 It -PRON- PRP 10_1101-2020_11_24_390039 305 2 is be VBZ 10_1101-2020_11_24_390039 305 3 The the DT 10_1101-2020_11_24_390039 305 4 copyright copyright NN 10_1101-2020_11_24_390039 305 5 holder holder NN 10_1101-2020_11_24_390039 305 6 for for IN 10_1101-2020_11_24_390039 305 7 this this DT 10_1101-2020_11_24_390039 305 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 305 9 version version NN 10_1101-2020_11_24_390039 305 10 posted post VBD 10_1101-2020_11_24_390039 305 11 January January NNP 10_1101-2020_11_24_390039 305 12 5 5 CD 10_1101-2020_11_24_390039 305 13 , , , 10_1101-2020_11_24_390039 305 14 2021 2021 CD 10_1101-2020_11_24_390039 305 15 . . . 10_1101-2020_11_24_390039 305 16 ; ; : 10_1101-2020_11_24_390039 305 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 305 18 : : : 10_1101-2020_11_24_390039 305 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 305 20 preprint preprint JJ 10_1101-2020_11_24_390039 305 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 305 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 305 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 305 24 - - HYPH 10_1101-2020_11_24_390039 305 25 F F NNP 10_1101-2020_11_24_390039 305 26 as as IN 10_1101-2020_11_24_390039 305 27 a a DT 10_1101-2020_11_24_390039 305 28 potential potential JJ 10_1101-2020_11_24_390039 305 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 305 30 agent agent NN 10_1101-2020_11_24_390039 305 31 Page Page NNP 10_1101-2020_11_24_390039 305 32 18 18 CD 10_1101-2020_11_24_390039 305 33 of of IN 10_1101-2020_11_24_390039 305 34 21 21 CD 10_1101-2020_11_24_390039 305 35 Sicari Sicari NNP 10_1101-2020_11_24_390039 305 36 , , , 10_1101-2020_11_24_390039 305 37 D. D. NNP 10_1101-2020_11_24_390039 305 38 , , , 10_1101-2020_11_24_390039 305 39 Chatziioannou Chatziioannou NNP 10_1101-2020_11_24_390039 305 40 , , , 10_1101-2020_11_24_390039 305 41 A. a. NN 10_1101-2020_11_24_390039 305 42 , , , 10_1101-2020_11_24_390039 305 43 Koutsandreas Koutsandreas NNP 10_1101-2020_11_24_390039 305 44 , , , 10_1101-2020_11_24_390039 305 45 T. T. NNP 10_1101-2020_11_24_390039 305 46 , , , 10_1101-2020_11_24_390039 305 47 Sitia Sitia NNP 10_1101-2020_11_24_390039 305 48 , , , 10_1101-2020_11_24_390039 305 49 R. R. NNP 10_1101-2020_11_24_390039 305 50 , , , 10_1101-2020_11_24_390039 305 51 and and CC 10_1101-2020_11_24_390039 305 52 Chevet Chevet NNP 10_1101-2020_11_24_390039 305 53 , , , 10_1101-2020_11_24_390039 305 54 E. E. NNP 10_1101-2020_11_24_390039 305 55 ( ( -LRB- 10_1101-2020_11_24_390039 305 56 2020 2020 CD 10_1101-2020_11_24_390039 305 57 ) ) -RRB- 10_1101-2020_11_24_390039 305 58 . . . 10_1101-2020_11_24_390039 306 1 Role role NN 10_1101-2020_11_24_390039 306 2 of of IN 10_1101-2020_11_24_390039 306 3 the the DT 10_1101-2020_11_24_390039 306 4 early early JJ 10_1101-2020_11_24_390039 306 5 secretory secretory JJ 10_1101-2020_11_24_390039 306 6 pathway pathway NN 10_1101-2020_11_24_390039 306 7 in in IN 10_1101-2020_11_24_390039 306 8 SARS SARS NNP 10_1101-2020_11_24_390039 306 9 - - HYPH 10_1101-2020_11_24_390039 306 10 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 306 11 infection infection NN 10_1101-2020_11_24_390039 306 12 . . . 10_1101-2020_11_24_390039 307 1 J. J. NNP 10_1101-2020_11_24_390039 308 1 Cell cell NN 10_1101-2020_11_24_390039 308 2 . . . 10_1101-2020_11_24_390039 309 1 Biol Biol NNP 10_1101-2020_11_24_390039 309 2 . . . 10_1101-2020_11_24_390039 310 1 219 219 CD 10_1101-2020_11_24_390039 310 2 , , , 10_1101-2020_11_24_390039 310 3 e202006005 e202006005 NNP 10_1101-2020_11_24_390039 310 4 . . . 10_1101-2020_11_24_390039 311 1 Shah Shah NNP 10_1101-2020_11_24_390039 311 2 , , , 10_1101-2020_11_24_390039 311 3 P. P. NNP 10_1101-2020_11_24_390039 311 4 S. S. NNP 10_1101-2020_11_24_390039 311 5 , , , 10_1101-2020_11_24_390039 311 6 Link Link NNP 10_1101-2020_11_24_390039 311 7 , , , 10_1101-2020_11_24_390039 311 8 N. N. NNP 10_1101-2020_11_24_390039 311 9 , , , 10_1101-2020_11_24_390039 311 10 Jang Jang NNP 10_1101-2020_11_24_390039 311 11 , , , 10_1101-2020_11_24_390039 311 12 G. G. NNP 10_1101-2020_11_24_390039 311 13 M. M. NNP 10_1101-2020_11_24_390039 311 14 , , , 10_1101-2020_11_24_390039 311 15 Sharp Sharp NNP 10_1101-2020_11_24_390039 311 16 , , , 10_1101-2020_11_24_390039 311 17 P. P. NNP 10_1101-2020_11_24_390039 311 18 P. P. NNP 10_1101-2020_11_24_390039 311 19 , , , 10_1101-2020_11_24_390039 311 20 Zhu Zhu NNP 10_1101-2020_11_24_390039 311 21 , , , 10_1101-2020_11_24_390039 311 22 T. T. NNP 10_1101-2020_11_24_390039 311 23 , , , 10_1101-2020_11_24_390039 311 24 Swaney Swaney NNP 10_1101-2020_11_24_390039 311 25 , , , 10_1101-2020_11_24_390039 311 26 D. D. NNP 10_1101-2020_11_24_390039 311 27 L. L. NNP 10_1101-2020_11_24_390039 311 28 , , , 10_1101-2020_11_24_390039 311 29 Johnson Johnson NNP 10_1101-2020_11_24_390039 311 30 , , , 10_1101-2020_11_24_390039 311 31 J. J. NNP 10_1101-2020_11_24_390039 311 32 R. R. NNP 10_1101-2020_11_24_390039 311 33 , , , 10_1101-2020_11_24_390039 311 34 Von Von NNP 10_1101-2020_11_24_390039 311 35 Dollen Dollen NNP 10_1101-2020_11_24_390039 311 36 , , , 10_1101-2020_11_24_390039 311 37 J. J. NNP 10_1101-2020_11_24_390039 311 38 , , , 10_1101-2020_11_24_390039 311 39 Ramage Ramage NNP 10_1101-2020_11_24_390039 311 40 , , , 10_1101-2020_11_24_390039 311 41 H. H. NNP 10_1101-2020_11_24_390039 311 42 R. R. NNP 10_1101-2020_11_24_390039 311 43 , , , 10_1101-2020_11_24_390039 311 44 Satkamp Satkamp NNP 10_1101-2020_11_24_390039 311 45 , , , 10_1101-2020_11_24_390039 311 46 L. L. NNP 10_1101-2020_11_24_390039 311 47 et et NNP 10_1101-2020_11_24_390039 311 48 al al NNP 10_1101-2020_11_24_390039 311 49 . . . 10_1101-2020_11_24_390039 312 1 ( ( -LRB- 10_1101-2020_11_24_390039 312 2 2018 2018 CD 10_1101-2020_11_24_390039 312 3 ) ) -RRB- 10_1101-2020_11_24_390039 312 4 . . . 10_1101-2020_11_24_390039 313 1 Comparative comparative JJ 10_1101-2020_11_24_390039 313 2 flavivirus flavivirus JJ 10_1101-2020_11_24_390039 313 3 - - HYPH 10_1101-2020_11_24_390039 313 4 host host NN 10_1101-2020_11_24_390039 313 5 protein protein NN 10_1101-2020_11_24_390039 313 6 interaction interaction NN 10_1101-2020_11_24_390039 313 7 mapping mapping NN 10_1101-2020_11_24_390039 313 8 reveals reveal VBZ 10_1101-2020_11_24_390039 313 9 mechanisms mechanism NNS 10_1101-2020_11_24_390039 313 10 of of IN 10_1101-2020_11_24_390039 313 11 Dengue Dengue NNP 10_1101-2020_11_24_390039 313 12 and and CC 10_1101-2020_11_24_390039 313 13 Zika Zika NNP 10_1101-2020_11_24_390039 313 14 virus virus NN 10_1101-2020_11_24_390039 313 15 pathogenesis pathogenesis NN 10_1101-2020_11_24_390039 313 16 . . . 10_1101-2020_11_24_390039 314 1 Cell cell NN 10_1101-2020_11_24_390039 314 2 . . . 10_1101-2020_11_24_390039 315 1 175 175 CD 10_1101-2020_11_24_390039 315 2 , , , 10_1101-2020_11_24_390039 315 3 1931 1931 CD 10_1101-2020_11_24_390039 315 4 - - SYM 10_1101-2020_11_24_390039 315 5 1945.e18 1945.e18 CD 10_1101-2020_11_24_390039 315 6 . . . 10_1101-2020_11_24_390039 316 1 Von Von NNP 10_1101-2020_11_24_390039 316 2 Heijne Heijne NNP 10_1101-2020_11_24_390039 316 3 , , , 10_1101-2020_11_24_390039 316 4 G. G. NNP 10_1101-2020_11_24_390039 316 5 ( ( -LRB- 10_1101-2020_11_24_390039 316 6 2007 2007 CD 10_1101-2020_11_24_390039 316 7 ) ) -RRB- 10_1101-2020_11_24_390039 316 8 . . . 10_1101-2020_11_24_390039 317 1 The the DT 10_1101-2020_11_24_390039 317 2 membrane membrane NN 10_1101-2020_11_24_390039 317 3 protein protein NN 10_1101-2020_11_24_390039 317 4 universe universe NN 10_1101-2020_11_24_390039 317 5 : : : 10_1101-2020_11_24_390039 317 6 what what WP 10_1101-2020_11_24_390039 317 7 ’s ’ VBZ 10_1101-2020_11_24_390039 317 8 out out RB 10_1101-2020_11_24_390039 317 9 there there RB 10_1101-2020_11_24_390039 317 10 and and CC 10_1101-2020_11_24_390039 317 11 why why WRB 10_1101-2020_11_24_390039 317 12 bother bother VB 10_1101-2020_11_24_390039 317 13 ? ? . 10_1101-2020_11_24_390039 318 1 J. J. NNP 10_1101-2020_11_24_390039 318 2 Intern Intern NNP 10_1101-2020_11_24_390039 318 3 . . . 10_1101-2020_11_24_390039 319 1 Med Med NNP 10_1101-2020_11_24_390039 319 2 . . . 10_1101-2020_11_24_390039 320 1 261 261 CD 10_1101-2020_11_24_390039 320 2 , , , 10_1101-2020_11_24_390039 320 3 543 543 CD 10_1101-2020_11_24_390039 320 4 - - SYM 10_1101-2020_11_24_390039 320 5 547 547 CD 10_1101-2020_11_24_390039 320 6 . . . 10_1101-2020_11_24_390039 321 1 Walls wall NNS 10_1101-2020_11_24_390039 321 2 , , , 10_1101-2020_11_24_390039 321 3 A. a. NN 10_1101-2020_11_24_390039 321 4 C. C. NNP 10_1101-2020_11_24_390039 321 5 , , , 10_1101-2020_11_24_390039 321 6 Park Park NNP 10_1101-2020_11_24_390039 321 7 , , , 10_1101-2020_11_24_390039 321 8 Y.-J. Y.-J. NNP 10_1101-2020_11_24_390039 321 9 , , , 10_1101-2020_11_24_390039 321 10 Tortorici Tortorici NNP 10_1101-2020_11_24_390039 321 11 , , , 10_1101-2020_11_24_390039 321 12 M. M. NNP 10_1101-2020_11_24_390039 321 13 A. A. NNP 10_1101-2020_11_24_390039 321 14 , , , 10_1101-2020_11_24_390039 321 15 Wall Wall NNP 10_1101-2020_11_24_390039 321 16 , , , 10_1101-2020_11_24_390039 321 17 A. A. NNP 10_1101-2020_11_24_390039 321 18 , , , 10_1101-2020_11_24_390039 321 19 McGuire McGuire NNP 10_1101-2020_11_24_390039 321 20 , , , 10_1101-2020_11_24_390039 321 21 A. a. NN 10_1101-2020_11_24_390039 321 22 T. t. NN 10_1101-2020_11_24_390039 321 23 , , , 10_1101-2020_11_24_390039 321 24 and and CC 10_1101-2020_11_24_390039 321 25 Veesler Veesler NNP 10_1101-2020_11_24_390039 321 26 , , , 10_1101-2020_11_24_390039 321 27 D. D. NNP 10_1101-2020_11_24_390039 321 28 ( ( -LRB- 10_1101-2020_11_24_390039 321 29 2020 2020 CD 10_1101-2020_11_24_390039 321 30 ) ) -RRB- 10_1101-2020_11_24_390039 321 31 . . . 10_1101-2020_11_24_390039 322 1 Structure structure NN 10_1101-2020_11_24_390039 322 2 , , , 10_1101-2020_11_24_390039 322 3 function function NN 10_1101-2020_11_24_390039 322 4 and and CC 10_1101-2020_11_24_390039 322 5 antigenicity antigenicity NN 10_1101-2020_11_24_390039 322 6 of of IN 10_1101-2020_11_24_390039 322 7 the the DT 10_1101-2020_11_24_390039 322 8 SARS SARS NNP 10_1101-2020_11_24_390039 322 9 - - HYPH 10_1101-2020_11_24_390039 322 10 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 322 11 spike spike NNP 10_1101-2020_11_24_390039 322 12 glycoprotein glycoprotein NNP 10_1101-2020_11_24_390039 322 13 . . . 10_1101-2020_11_24_390039 323 1 Cell cell NN 10_1101-2020_11_24_390039 323 2 . . . 10_1101-2020_11_24_390039 324 1 181 181 CD 10_1101-2020_11_24_390039 324 2 , , , 10_1101-2020_11_24_390039 324 3 P281 P281 NNP 10_1101-2020_11_24_390039 324 4 - - HYPH 10_1101-2020_11_24_390039 324 5 292.E6 292.E6 NNP 10_1101-2020_11_24_390039 324 6 . . . 10_1101-2020_11_24_390039 325 1 Warner Warner NNP 10_1101-2020_11_24_390039 325 2 , , , 10_1101-2020_11_24_390039 325 3 F. F. NNP 10_1101-2020_11_24_390039 325 4 J. J. NNP 10_1101-2020_11_24_390039 325 5 , , , 10_1101-2020_11_24_390039 325 6 Lew Lew NNP 10_1101-2020_11_24_390039 325 7 , , , 10_1101-2020_11_24_390039 325 8 R. R. NNP 10_1101-2020_11_24_390039 325 9 A. A. NNP 10_1101-2020_11_24_390039 325 10 , , , 10_1101-2020_11_24_390039 325 11 Smith Smith NNP 10_1101-2020_11_24_390039 325 12 , , , 10_1101-2020_11_24_390039 325 13 A. a. NN 10_1101-2020_11_24_390039 325 14 I. I. NNP 10_1101-2020_11_24_390039 325 15 , , , 10_1101-2020_11_24_390039 325 16 Lambert Lambert NNP 10_1101-2020_11_24_390039 325 17 , , , 10_1101-2020_11_24_390039 325 18 D. D. NNP 10_1101-2020_11_24_390039 325 19 W. W. NNP 10_1101-2020_11_24_390039 325 20 , , , 10_1101-2020_11_24_390039 325 21 Hooper Hooper NNP 10_1101-2020_11_24_390039 325 22 , , , 10_1101-2020_11_24_390039 325 23 N. N. NNP 10_1101-2020_11_24_390039 325 24 M. M. NNP 10_1101-2020_11_24_390039 325 25 , , , 10_1101-2020_11_24_390039 325 26 and and CC 10_1101-2020_11_24_390039 325 27 Turner Turner NNP 10_1101-2020_11_24_390039 325 28 , , , 10_1101-2020_11_24_390039 325 29 A. a. NN 10_1101-2020_11_24_390039 325 30 T. t. NN 10_1101-2020_11_24_390039 325 31 ( ( -LRB- 10_1101-2020_11_24_390039 325 32 2005 2005 CD 10_1101-2020_11_24_390039 325 33 ) ) -RRB- 10_1101-2020_11_24_390039 325 34 . . . 10_1101-2020_11_24_390039 326 1 Angiotensin Angiotensin NNP 10_1101-2020_11_24_390039 326 2 - - HYPH 10_1101-2020_11_24_390039 326 3 converting convert VBG 10_1101-2020_11_24_390039 326 4 enzyme enzyme NNS 10_1101-2020_11_24_390039 326 5 2 2 CD 10_1101-2020_11_24_390039 326 6 ( ( -LRB- 10_1101-2020_11_24_390039 326 7 ACE2 ACE2 NNP 10_1101-2020_11_24_390039 326 8 ) ) -RRB- 10_1101-2020_11_24_390039 326 9 , , , 10_1101-2020_11_24_390039 326 10 but but CC 10_1101-2020_11_24_390039 326 11 not not RB 10_1101-2020_11_24_390039 326 12 ACE ACE NNP 10_1101-2020_11_24_390039 326 13 , , , 10_1101-2020_11_24_390039 326 14 is be VBZ 10_1101-2020_11_24_390039 326 15 preferentially preferentially RB 10_1101-2020_11_24_390039 326 16 localised localise VBN 10_1101-2020_11_24_390039 326 17 to to IN 10_1101-2020_11_24_390039 326 18 the the DT 10_1101-2020_11_24_390039 326 19 apical apical JJ 10_1101-2020_11_24_390039 326 20 surface surface NN 10_1101-2020_11_24_390039 326 21 of of IN 10_1101-2020_11_24_390039 326 22 polarised polarise VBN 10_1101-2020_11_24_390039 326 23 kidney kidney NN 10_1101-2020_11_24_390039 326 24 cells cell NNS 10_1101-2020_11_24_390039 326 25 . . . 10_1101-2020_11_24_390039 327 1 J. J. NNP 10_1101-2020_11_24_390039 327 2 Biol Biol NNP 10_1101-2020_11_24_390039 327 3 . . . 10_1101-2020_11_24_390039 328 1 Chem Chem NNP 10_1101-2020_11_24_390039 328 2 . . . 10_1101-2020_11_24_390039 329 1 280 280 CD 10_1101-2020_11_24_390039 329 2 , , , 10_1101-2020_11_24_390039 329 3 39353 39353 CD 10_1101-2020_11_24_390039 329 4 - - SYM 10_1101-2020_11_24_390039 329 5 39362 39362 CD 10_1101-2020_11_24_390039 329 6 . . . 10_1101-2020_11_24_390039 330 1 Wilson Wilson NNP 10_1101-2020_11_24_390039 330 2 , , , 10_1101-2020_11_24_390039 330 3 C. C. NNP 10_1101-2020_11_24_390039 330 4 M. M. NNP 10_1101-2020_11_24_390039 330 5 , , , 10_1101-2020_11_24_390039 330 6 and and CC 10_1101-2020_11_24_390039 330 7 High High NNP 10_1101-2020_11_24_390039 330 8 , , , 10_1101-2020_11_24_390039 330 9 S. S. NNP 10_1101-2020_11_24_390039 330 10 ( ( -LRB- 10_1101-2020_11_24_390039 330 11 2007 2007 CD 10_1101-2020_11_24_390039 330 12 ) ) -RRB- 10_1101-2020_11_24_390039 330 13 . . . 10_1101-2020_11_24_390039 331 1 Ribophorin Ribophorin NNP 10_1101-2020_11_24_390039 331 2 I -PRON- PRP 10_1101-2020_11_24_390039 331 3 acts act VBZ 10_1101-2020_11_24_390039 331 4 as as IN 10_1101-2020_11_24_390039 331 5 a a DT 10_1101-2020_11_24_390039 331 6 substrate substrate NN 10_1101-2020_11_24_390039 331 7 - - HYPH 10_1101-2020_11_24_390039 331 8 specific specific JJ 10_1101-2020_11_24_390039 331 9 facilitator facilitator NN 10_1101-2020_11_24_390039 331 10 of of IN 10_1101-2020_11_24_390039 331 11 N n NN 10_1101-2020_11_24_390039 331 12 - - HYPH 10_1101-2020_11_24_390039 331 13 glycosylation glycosylation NN 10_1101-2020_11_24_390039 331 14 . . . 10_1101-2020_11_24_390039 332 1 J. J. NNP 10_1101-2020_11_24_390039 333 1 Cell cell NN 10_1101-2020_11_24_390039 333 2 . . . 10_1101-2020_11_24_390039 334 1 Sci Sci NNP 10_1101-2020_11_24_390039 334 2 . . . 10_1101-2020_11_24_390039 335 1 120 120 CD 10_1101-2020_11_24_390039 335 2 , , , 10_1101-2020_11_24_390039 335 3 648 648 CD 10_1101-2020_11_24_390039 335 4 - - SYM 10_1101-2020_11_24_390039 335 5 657 657 CD 10_1101-2020_11_24_390039 335 6 . . . 10_1101-2020_11_24_390039 336 1 Young Young NNP 10_1101-2020_11_24_390039 336 2 , , , 10_1101-2020_11_24_390039 336 3 B. B. NNP 10_1101-2020_11_24_390039 336 4 E. E. NNP 10_1101-2020_11_24_390039 336 5 , , , 10_1101-2020_11_24_390039 336 6 Fong Fong NNP 10_1101-2020_11_24_390039 336 7 , , , 10_1101-2020_11_24_390039 336 8 S.-W. S.-W. NNP 10_1101-2020_11_24_390039 336 9 , , , 10_1101-2020_11_24_390039 336 10 Chan Chan NNP 10_1101-2020_11_24_390039 336 11 , , , 10_1101-2020_11_24_390039 336 12 Y. Y. NNP 10_1101-2020_11_24_390039 336 13 H. H. NNP 10_1101-2020_11_24_390039 336 14 , , , 10_1101-2020_11_24_390039 336 15 Mak Mak NNP 10_1101-2020_11_24_390039 336 16 , , , 10_1101-2020_11_24_390039 336 17 T.-M. T.-M. NNP 10_1101-2020_11_24_390039 336 18 , , , 10_1101-2020_11_24_390039 336 19 Ang Ang NNP 10_1101-2020_11_24_390039 336 20 , , , 10_1101-2020_11_24_390039 336 21 L. L. NNP 10_1101-2020_11_24_390039 336 22 W. W. NNP 10_1101-2020_11_24_390039 336 23 , , , 10_1101-2020_11_24_390039 336 24 Anderson Anderson NNP 10_1101-2020_11_24_390039 336 25 , , , 10_1101-2020_11_24_390039 336 26 D. D. NNP 10_1101-2020_11_24_390039 336 27 E. E. NNP 10_1101-2020_11_24_390039 336 28 , , , 10_1101-2020_11_24_390039 336 29 Yi Yi NNP 10_1101-2020_11_24_390039 336 30 - - HYPH 10_1101-2020_11_24_390039 336 31 Pin Pin NNP 10_1101-2020_11_24_390039 336 32 Lee Lee NNP 10_1101-2020_11_24_390039 336 33 , , , 10_1101-2020_11_24_390039 336 34 C. C. NNP 10_1101-2020_11_24_390039 336 35 , , , 10_1101-2020_11_24_390039 336 36 Naqiah Naqiah NNP 10_1101-2020_11_24_390039 336 37 Amrun Amrun NNP 10_1101-2020_11_24_390039 336 38 , , , 10_1101-2020_11_24_390039 336 39 S. S. NNP 10_1101-2020_11_24_390039 336 40 , , , 10_1101-2020_11_24_390039 336 41 Lee Lee NNP 10_1101-2020_11_24_390039 336 42 , , , 10_1101-2020_11_24_390039 336 43 B. B. NNP 10_1101-2020_11_24_390039 336 44 , , , 10_1101-2020_11_24_390039 336 45 Shan Shan NNP 10_1101-2020_11_24_390039 336 46 Goh Goh NNP 10_1101-2020_11_24_390039 336 47 , , , 10_1101-2020_11_24_390039 336 48 Y. Y. NNP 10_1101-2020_11_24_390039 336 49 et et NNP 10_1101-2020_11_24_390039 336 50 al al NNP 10_1101-2020_11_24_390039 336 51 . . . 10_1101-2020_11_24_390039 337 1 ( ( -LRB- 10_1101-2020_11_24_390039 337 2 2020 2020 CD 10_1101-2020_11_24_390039 337 3 ) ) -RRB- 10_1101-2020_11_24_390039 337 4 . . . 10_1101-2020_11_24_390039 338 1 Effects effect NNS 10_1101-2020_11_24_390039 338 2 of of IN 10_1101-2020_11_24_390039 338 3 a a DT 10_1101-2020_11_24_390039 338 4 major major JJ 10_1101-2020_11_24_390039 338 5 deletion deletion NN 10_1101-2020_11_24_390039 338 6 in in IN 10_1101-2020_11_24_390039 338 7 the the DT 10_1101-2020_11_24_390039 338 8 SARS SARS NNP 10_1101-2020_11_24_390039 338 9 - - HYPH 10_1101-2020_11_24_390039 338 10 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 338 11 genome genome NN 10_1101-2020_11_24_390039 338 12 on on IN 10_1101-2020_11_24_390039 338 13 the the DT 10_1101-2020_11_24_390039 338 14 severity severity NN 10_1101-2020_11_24_390039 338 15 of of IN 10_1101-2020_11_24_390039 338 16 infection infection NN 10_1101-2020_11_24_390039 338 17 and and CC 10_1101-2020_11_24_390039 338 18 inflammatory inflammatory JJ 10_1101-2020_11_24_390039 338 19 response response NN 10_1101-2020_11_24_390039 338 20 : : : 10_1101-2020_11_24_390039 338 21 an an DT 10_1101-2020_11_24_390039 338 22 observational observational JJ 10_1101-2020_11_24_390039 338 23 cohort cohort NN 10_1101-2020_11_24_390039 338 24 study study NN 10_1101-2020_11_24_390039 338 25 . . . 10_1101-2020_11_24_390039 339 1 Lancet Lancet NNP 10_1101-2020_11_24_390039 339 2 . . . 10_1101-2020_11_24_390039 340 1 396 396 CD 10_1101-2020_11_24_390039 340 2 , , , 10_1101-2020_11_24_390039 340 3 603 603 CD 10_1101-2020_11_24_390039 340 4 - - SYM 10_1101-2020_11_24_390039 340 5 611 611 CD 10_1101-2020_11_24_390039 340 6 . . . 10_1101-2020_11_24_390039 341 1 Zhang Zhang NNP 10_1101-2020_11_24_390039 341 2 , , , 10_1101-2020_11_24_390039 341 3 Y. Y. NNP 10_1101-2020_11_24_390039 341 4 , , , 10_1101-2020_11_24_390039 341 5 Zhang Zhang NNP 10_1101-2020_11_24_390039 341 6 , , , 10_1101-2020_11_24_390039 341 7 J. J. NNP 10_1101-2020_11_24_390039 341 8 , , , 10_1101-2020_11_24_390039 341 9 Chen Chen NNP 10_1101-2020_11_24_390039 341 10 . . . 10_1101-2020_11_24_390039 342 1 Y. Y. NNP 10_1101-2020_11_24_390039 342 2 , , , 10_1101-2020_11_24_390039 342 3 Luo Luo NNP 10_1101-2020_11_24_390039 342 4 , , , 10_1101-2020_11_24_390039 342 5 B. B. NNP 10_1101-2020_11_24_390039 342 6 , , , 10_1101-2020_11_24_390039 342 7 Yuan Yuan NNP 10_1101-2020_11_24_390039 342 8 , , , 10_1101-2020_11_24_390039 342 9 Y. Y. NNP 10_1101-2020_11_24_390039 342 10 , , , 10_1101-2020_11_24_390039 342 11 Huang Huang NNP 10_1101-2020_11_24_390039 342 12 , , , 10_1101-2020_11_24_390039 342 13 F. F. NNP 10_1101-2020_11_24_390039 342 14 , , , 10_1101-2020_11_24_390039 342 15 Yang Yang NNP 10_1101-2020_11_24_390039 342 16 , , , 10_1101-2020_11_24_390039 342 17 T. T. NNP 10_1101-2020_11_24_390039 342 18 , , , 10_1101-2020_11_24_390039 342 19 Yu Yu NNP 10_1101-2020_11_24_390039 342 20 , , , 10_1101-2020_11_24_390039 342 21 F. F. NNP 10_1101-2020_11_24_390039 342 22 , , , 10_1101-2020_11_24_390039 342 23 Liu Liu NNP 10_1101-2020_11_24_390039 342 24 , , , 10_1101-2020_11_24_390039 342 25 J. J. NNP 10_1101-2020_11_24_390039 342 26 , , , 10_1101-2020_11_24_390039 342 27 Song Song NNP 10_1101-2020_11_24_390039 342 28 , , , 10_1101-2020_11_24_390039 342 29 Z. Z. NNP 10_1101-2020_11_24_390039 342 30 et et FW 10_1101-2020_11_24_390039 342 31 al al NNP 10_1101-2020_11_24_390039 342 32 . . . 10_1101-2020_11_24_390039 343 1 ( ( -LRB- 10_1101-2020_11_24_390039 343 2 2020 2020 CD 10_1101-2020_11_24_390039 343 3 ) ) -RRB- 10_1101-2020_11_24_390039 343 4 . . . 10_1101-2020_11_24_390039 344 1 The the DT 10_1101-2020_11_24_390039 344 2 ORF8 orf8 JJ 10_1101-2020_11_24_390039 344 3 protein protein NN 10_1101-2020_11_24_390039 344 4 of of IN 10_1101-2020_11_24_390039 344 5 SARS SARS NNP 10_1101-2020_11_24_390039 344 6 - - HYPH 10_1101-2020_11_24_390039 344 7 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 344 8 mediates mediate VBZ 10_1101-2020_11_24_390039 344 9 immune immune JJ 10_1101-2020_11_24_390039 344 10 evasion evasion NN 10_1101-2020_11_24_390039 344 11 through through IN 10_1101-2020_11_24_390039 344 12 potently potently RB 10_1101-2020_11_24_390039 344 13 downregulating downregulate VBG 10_1101-2020_11_24_390039 344 14 MHC-1 MHC-1 NNP 10_1101-2020_11_24_390039 344 15 . . . 10_1101-2020_11_24_390039 345 1 bioRxiv biorxiv NN 10_1101-2020_11_24_390039 345 2 . . . 10_1101-2020_11_24_390039 346 1 doi doi NNP 10_1101-2020_11_24_390039 346 2 : : : 10_1101-2020_11_24_390039 346 3 10.1101/2020.05.24.111823 10.1101/2020.05.24.111823 CD 10_1101-2020_11_24_390039 346 4 .CC .CC : 10_1101-2020_11_24_390039 346 5 - - : 10_1101-2020_11_24_390039 346 6 BY by IN 10_1101-2020_11_24_390039 346 7 - - HYPH 10_1101-2020_11_24_390039 346 8 ND ND NNP 10_1101-2020_11_24_390039 346 9 4.0 4.0 CD 10_1101-2020_11_24_390039 346 10 International international JJ 10_1101-2020_11_24_390039 346 11 licensemade licensemade NN 10_1101-2020_11_24_390039 346 12 available available JJ 10_1101-2020_11_24_390039 346 13 under under IN 10_1101-2020_11_24_390039 346 14 a a DT 10_1101-2020_11_24_390039 346 15 ( ( -LRB- 10_1101-2020_11_24_390039 346 16 which which WDT 10_1101-2020_11_24_390039 346 17 was be VBD 10_1101-2020_11_24_390039 346 18 not not RB 10_1101-2020_11_24_390039 346 19 certified certify VBN 10_1101-2020_11_24_390039 346 20 by by IN 10_1101-2020_11_24_390039 346 21 peer peer NN 10_1101-2020_11_24_390039 346 22 review review NN 10_1101-2020_11_24_390039 346 23 ) ) -RRB- 10_1101-2020_11_24_390039 346 24 is be VBZ 10_1101-2020_11_24_390039 346 25 the the DT 10_1101-2020_11_24_390039 346 26 author author NN 10_1101-2020_11_24_390039 346 27 / / SYM 10_1101-2020_11_24_390039 346 28 funder funder NN 10_1101-2020_11_24_390039 346 29 , , , 10_1101-2020_11_24_390039 346 30 who who WP 10_1101-2020_11_24_390039 346 31 has have VBZ 10_1101-2020_11_24_390039 346 32 granted grant VBN 10_1101-2020_11_24_390039 346 33 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 346 34 a a DT 10_1101-2020_11_24_390039 346 35 license license NN 10_1101-2020_11_24_390039 346 36 to to TO 10_1101-2020_11_24_390039 346 37 display display VB 10_1101-2020_11_24_390039 346 38 the the DT 10_1101-2020_11_24_390039 346 39 preprint preprint NN 10_1101-2020_11_24_390039 346 40 in in IN 10_1101-2020_11_24_390039 346 41 perpetuity perpetuity NN 10_1101-2020_11_24_390039 346 42 . . . 10_1101-2020_11_24_390039 347 1 It -PRON- PRP 10_1101-2020_11_24_390039 347 2 is be VBZ 10_1101-2020_11_24_390039 347 3 The the DT 10_1101-2020_11_24_390039 347 4 copyright copyright NN 10_1101-2020_11_24_390039 347 5 holder holder NN 10_1101-2020_11_24_390039 347 6 for for IN 10_1101-2020_11_24_390039 347 7 this this DT 10_1101-2020_11_24_390039 347 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 347 9 version version NN 10_1101-2020_11_24_390039 347 10 posted post VBD 10_1101-2020_11_24_390039 347 11 January January NNP 10_1101-2020_11_24_390039 347 12 5 5 CD 10_1101-2020_11_24_390039 347 13 , , , 10_1101-2020_11_24_390039 347 14 2021 2021 CD 10_1101-2020_11_24_390039 347 15 . . . 10_1101-2020_11_24_390039 347 16 ; ; : 10_1101-2020_11_24_390039 347 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 347 18 : : : 10_1101-2020_11_24_390039 347 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 347 20 preprint preprint JJ 10_1101-2020_11_24_390039 347 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 347 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 347 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 347 24 - - HYPH 10_1101-2020_11_24_390039 347 25 F F NNP 10_1101-2020_11_24_390039 347 26 as as IN 10_1101-2020_11_24_390039 347 27 a a DT 10_1101-2020_11_24_390039 347 28 potential potential JJ 10_1101-2020_11_24_390039 347 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 347 30 agent agent NN 10_1101-2020_11_24_390039 347 31 Page Page NNP 10_1101-2020_11_24_390039 347 32 19 19 CD 10_1101-2020_11_24_390039 347 33 of of IN 10_1101-2020_11_24_390039 347 34 21 21 CD 10_1101-2020_11_24_390039 347 35 Zhou Zhou NNP 10_1101-2020_11_24_390039 347 36 , , , 10_1101-2020_11_24_390039 347 37 P. P. NNP 10_1101-2020_11_24_390039 347 38 , , , 10_1101-2020_11_24_390039 347 39 Yang Yang NNP 10_1101-2020_11_24_390039 347 40 , , , 10_1101-2020_11_24_390039 347 41 X.-L. X.-L. NNP 10_1101-2020_11_24_390039 347 42 , , , 10_1101-2020_11_24_390039 347 43 Wang Wang NNP 10_1101-2020_11_24_390039 347 44 , , , 10_1101-2020_11_24_390039 347 45 X.-G. X.-G. NNP 10_1101-2020_11_24_390039 347 46 , , , 10_1101-2020_11_24_390039 347 47 Hu Hu NNP 10_1101-2020_11_24_390039 347 48 , , , 10_1101-2020_11_24_390039 347 49 B. B. NNP 10_1101-2020_11_24_390039 347 50 , , , 10_1101-2020_11_24_390039 347 51 Zhang Zhang NNP 10_1101-2020_11_24_390039 347 52 , , , 10_1101-2020_11_24_390039 347 53 L. L. NNP 10_1101-2020_11_24_390039 347 54 , , , 10_1101-2020_11_24_390039 347 55 Zhang Zhang NNP 10_1101-2020_11_24_390039 347 56 , , , 10_1101-2020_11_24_390039 347 57 W. W. NNP 10_1101-2020_11_24_390039 347 58 , , , 10_1101-2020_11_24_390039 347 59 Si Si NNP 10_1101-2020_11_24_390039 347 60 , , , 10_1101-2020_11_24_390039 347 61 H.-R. H.-R. NNP 10_1101-2020_11_24_390039 347 62 , , , 10_1101-2020_11_24_390039 347 63 Zhu Zhu NNP 10_1101-2020_11_24_390039 347 64 , , , 10_1101-2020_11_24_390039 347 65 Y. Y. NNP 10_1101-2020_11_24_390039 347 66 , , , 10_1101-2020_11_24_390039 347 67 Li Li NNP 10_1101-2020_11_24_390039 347 68 , , , 10_1101-2020_11_24_390039 347 69 B. B. NNP 10_1101-2020_11_24_390039 347 70 , , , 10_1101-2020_11_24_390039 347 71 Huang Huang NNP 10_1101-2020_11_24_390039 347 72 , , , 10_1101-2020_11_24_390039 347 73 C.-L. C.-L. NNP 10_1101-2020_11_24_390039 347 74 et et FW 10_1101-2020_11_24_390039 347 75 al al NNP 10_1101-2020_11_24_390039 347 76 . . . 10_1101-2020_11_24_390039 348 1 ( ( -LRB- 10_1101-2020_11_24_390039 348 2 2020 2020 CD 10_1101-2020_11_24_390039 348 3 ) ) -RRB- 10_1101-2020_11_24_390039 348 4 . . . 10_1101-2020_11_24_390039 349 1 A a DT 10_1101-2020_11_24_390039 349 2 pneumonia pneumonia NN 10_1101-2020_11_24_390039 349 3 outbreak outbreak NN 10_1101-2020_11_24_390039 349 4 associated associate VBN 10_1101-2020_11_24_390039 349 5 with with IN 10_1101-2020_11_24_390039 349 6 a a DT 10_1101-2020_11_24_390039 349 7 new new JJ 10_1101-2020_11_24_390039 349 8 coronavirus coronavirus NN 10_1101-2020_11_24_390039 349 9 of of IN 10_1101-2020_11_24_390039 349 10 probable probable JJ 10_1101-2020_11_24_390039 349 11 bat bat NN 10_1101-2020_11_24_390039 349 12 origin origin NN 10_1101-2020_11_24_390039 349 13 . . . 10_1101-2020_11_24_390039 350 1 Nature nature NN 10_1101-2020_11_24_390039 350 2 . . . 10_1101-2020_11_24_390039 351 1 579 579 CD 10_1101-2020_11_24_390039 351 2 , , , 10_1101-2020_11_24_390039 351 3 270 270 CD 10_1101-2020_11_24_390039 351 4 - - SYM 10_1101-2020_11_24_390039 351 5 273 273 CD 10_1101-2020_11_24_390039 351 6 . . . 10_1101-2020_11_24_390039 352 1 Zhu Zhu NNP 10_1101-2020_11_24_390039 352 2 , , , 10_1101-2020_11_24_390039 352 3 N. N. NNP 10_1101-2020_11_24_390039 352 4 , , , 10_1101-2020_11_24_390039 352 5 Zhang Zhang NNP 10_1101-2020_11_24_390039 352 6 , , , 10_1101-2020_11_24_390039 352 7 D. D. NNP 10_1101-2020_11_24_390039 352 8 , , , 10_1101-2020_11_24_390039 352 9 Wang Wang NNP 10_1101-2020_11_24_390039 352 10 , , , 10_1101-2020_11_24_390039 352 11 W. W. NNP 10_1101-2020_11_24_390039 352 12 , , , 10_1101-2020_11_24_390039 352 13 Li Li NNP 10_1101-2020_11_24_390039 352 14 , , , 10_1101-2020_11_24_390039 352 15 X. X. NNP 10_1101-2020_11_24_390039 352 16 , , , 10_1101-2020_11_24_390039 352 17 Yang Yang NNP 10_1101-2020_11_24_390039 352 18 , , , 10_1101-2020_11_24_390039 352 19 B. B. NNP 10_1101-2020_11_24_390039 352 20 , , , 10_1101-2020_11_24_390039 352 21 Song Song NNP 10_1101-2020_11_24_390039 352 22 , , , 10_1101-2020_11_24_390039 352 23 J. J. NNP 10_1101-2020_11_24_390039 352 24 , , , 10_1101-2020_11_24_390039 352 25 Zhao Zhao NNP 10_1101-2020_11_24_390039 352 26 , , , 10_1101-2020_11_24_390039 352 27 X. X. NNP 10_1101-2020_11_24_390039 352 28 , , , 10_1101-2020_11_24_390039 352 29 Huang Huang NNP 10_1101-2020_11_24_390039 352 30 , , , 10_1101-2020_11_24_390039 352 31 B. B. NNP 10_1101-2020_11_24_390039 352 32 , , , 10_1101-2020_11_24_390039 352 33 Shi Shi NNP 10_1101-2020_11_24_390039 352 34 , , , 10_1101-2020_11_24_390039 352 35 W. W. NNP 10_1101-2020_11_24_390039 352 36 , , , 10_1101-2020_11_24_390039 352 37 Lu Lu NNP 10_1101-2020_11_24_390039 352 38 , , , 10_1101-2020_11_24_390039 352 39 R. R. NNP 10_1101-2020_11_24_390039 352 40 , , , 10_1101-2020_11_24_390039 352 41 et et NNP 10_1101-2020_11_24_390039 352 42 al al NNP 10_1101-2020_11_24_390039 352 43 . . . 10_1101-2020_11_24_390039 353 1 ( ( -LRB- 10_1101-2020_11_24_390039 353 2 2020 2020 CD 10_1101-2020_11_24_390039 353 3 ) ) -RRB- 10_1101-2020_11_24_390039 353 4 . . . 10_1101-2020_11_24_390039 354 1 A a DT 10_1101-2020_11_24_390039 354 2 novel novel JJ 10_1101-2020_11_24_390039 354 3 coronavirus coronavirus NN 10_1101-2020_11_24_390039 354 4 from from IN 10_1101-2020_11_24_390039 354 5 patients patient NNS 10_1101-2020_11_24_390039 354 6 with with IN 10_1101-2020_11_24_390039 354 7 pneumonia pneumonia NN 10_1101-2020_11_24_390039 354 8 in in IN 10_1101-2020_11_24_390039 354 9 China China NNP 10_1101-2020_11_24_390039 354 10 , , , 10_1101-2020_11_24_390039 354 11 2019 2019 CD 10_1101-2020_11_24_390039 354 12 . . . 10_1101-2020_11_24_390039 355 1 N. N. NNP 10_1101-2020_11_24_390039 355 2 Eng Eng NNP 10_1101-2020_11_24_390039 355 3 . . . 10_1101-2020_11_24_390039 356 1 J. J. NNP 10_1101-2020_11_24_390039 356 2 Med Med NNP 10_1101-2020_11_24_390039 356 3 . . . 10_1101-2020_11_24_390039 357 1 382 382 CD 10_1101-2020_11_24_390039 357 2 , , , 10_1101-2020_11_24_390039 357 3 727 727 CD 10_1101-2020_11_24_390039 357 4 - - SYM 10_1101-2020_11_24_390039 357 5 733 733 CD 10_1101-2020_11_24_390039 357 6 . . . 10_1101-2020_11_24_390039 358 1 Zong Zong NNP 10_1101-2020_11_24_390039 358 2 , , , 10_1101-2020_11_24_390039 358 3 G. G. NNP 10_1101-2020_11_24_390039 358 4 , , , 10_1101-2020_11_24_390039 358 5 Hu Hu NNP 10_1101-2020_11_24_390039 358 6 , , , 10_1101-2020_11_24_390039 358 7 Z. Z. NNP 10_1101-2020_11_24_390039 358 8 , , , 10_1101-2020_11_24_390039 358 9 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 358 10 , , , 10_1101-2020_11_24_390039 358 11 S. S. NNP 10_1101-2020_11_24_390039 358 12 , , , 10_1101-2020_11_24_390039 358 13 Tranter Tranter NNP 10_1101-2020_11_24_390039 358 14 , , , 10_1101-2020_11_24_390039 358 15 D. D. NNP 10_1101-2020_11_24_390039 358 16 , , , 10_1101-2020_11_24_390039 358 17 Iannotti Iannotti NNP 10_1101-2020_11_24_390039 358 18 , , , 10_1101-2020_11_24_390039 358 19 M. M. NNP 10_1101-2020_11_24_390039 358 20 J. J. NNP 10_1101-2020_11_24_390039 358 21 , , , 10_1101-2020_11_24_390039 358 22 Baron Baron NNP 10_1101-2020_11_24_390039 358 23 , , , 10_1101-2020_11_24_390039 358 24 L. L. NNP 10_1101-2020_11_24_390039 358 25 , , , 10_1101-2020_11_24_390039 358 26 Hall Hall NNP 10_1101-2020_11_24_390039 358 27 , , , 10_1101-2020_11_24_390039 358 28 B. B. NNP 10_1101-2020_11_24_390039 358 29 , , , 10_1101-2020_11_24_390039 358 30 Corfield Corfield NNP 10_1101-2020_11_24_390039 358 31 , , , 10_1101-2020_11_24_390039 358 32 K. K. NNP 10_1101-2020_11_24_390039 358 33 , , , 10_1101-2020_11_24_390039 358 34 Paatero Paatero NNP 10_1101-2020_11_24_390039 358 35 , , , 10_1101-2020_11_24_390039 358 36 A. a. NN 10_1101-2020_11_24_390039 358 37 , , , 10_1101-2020_11_24_390039 358 38 Henderson Henderson NNP 10_1101-2020_11_24_390039 358 39 M. M. NNP 10_1101-2020_11_24_390039 358 40 et et NNP 10_1101-2020_11_24_390039 358 41 al al NNP 10_1101-2020_11_24_390039 358 42 . . . 10_1101-2020_11_24_390039 359 1 ( ( -LRB- 10_1101-2020_11_24_390039 359 2 2019 2019 CD 10_1101-2020_11_24_390039 359 3 ) ) -RRB- 10_1101-2020_11_24_390039 359 4 . . . 10_1101-2020_11_24_390039 360 1 Ipomoeassin Ipomoeassin NNP 10_1101-2020_11_24_390039 360 2 F F NNP 10_1101-2020_11_24_390039 360 3 binds bind NNS 10_1101-2020_11_24_390039 360 4 Sec61α Sec61α NNP 10_1101-2020_11_24_390039 360 5 to to TO 10_1101-2020_11_24_390039 360 6 inhibit inhibit VB 10_1101-2020_11_24_390039 360 7 protein protein NN 10_1101-2020_11_24_390039 360 8 translocation translocation NN 10_1101-2020_11_24_390039 360 9 . . . 10_1101-2020_11_24_390039 361 1 J. J. NNP 10_1101-2020_11_24_390039 362 1 Am Am NNP 10_1101-2020_11_24_390039 362 2 . . . 10_1101-2020_11_24_390039 363 1 Chem Chem NNP 10_1101-2020_11_24_390039 363 2 . . . 10_1101-2020_11_24_390039 364 1 Soc Soc NNP 10_1101-2020_11_24_390039 364 2 . . . 10_1101-2020_11_24_390039 365 1 141 141 CD 10_1101-2020_11_24_390039 365 2 , , , 10_1101-2020_11_24_390039 365 3 8450 8450 CD 10_1101-2020_11_24_390039 365 4 - - SYM 10_1101-2020_11_24_390039 365 5 8461 8461 CD 10_1101-2020_11_24_390039 365 6 . . . 10_1101-2020_11_24_390039 366 1 Zong Zong NNP 10_1101-2020_11_24_390039 366 2 , , , 10_1101-2020_11_24_390039 366 3 G. G. NNP 10_1101-2020_11_24_390039 366 4 , , , 10_1101-2020_11_24_390039 366 5 Hu Hu NNP 10_1101-2020_11_24_390039 366 6 , , , 10_1101-2020_11_24_390039 366 7 Z. Z. NNP 10_1101-2020_11_24_390039 366 8 , , , 10_1101-2020_11_24_390039 366 9 Duah Duah NNP 10_1101-2020_11_24_390039 366 10 , , , 10_1101-2020_11_24_390039 366 11 K. K. NNP 10_1101-2020_11_24_390039 366 12 , , , 10_1101-2020_11_24_390039 366 13 B. B. NNP 10_1101-2020_11_24_390039 366 14 , , , 10_1101-2020_11_24_390039 366 15 Andrews Andrews NNP 10_1101-2020_11_24_390039 366 16 , , , 10_1101-2020_11_24_390039 366 17 L. L. NNP 10_1101-2020_11_24_390039 366 18 E. E. NNP 10_1101-2020_11_24_390039 366 19 , , , 10_1101-2020_11_24_390039 366 20 Zhou Zhou NNP 10_1101-2020_11_24_390039 366 21 , , , 10_1101-2020_11_24_390039 366 22 J. J. NNP 10_1101-2020_11_24_390039 366 23 , , , 10_1101-2020_11_24_390039 366 24 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 366 25 , , , 10_1101-2020_11_24_390039 366 26 S. S. NNP 10_1101-2020_11_24_390039 366 27 , , , 10_1101-2020_11_24_390039 366 28 Whisenhunt Whisenhunt NNP 10_1101-2020_11_24_390039 366 29 , , , 10_1101-2020_11_24_390039 366 30 L. L. NNP 10_1101-2020_11_24_390039 366 31 , , , 10_1101-2020_11_24_390039 366 32 Shim Shim NNP 10_1101-2020_11_24_390039 366 33 , , , 10_1101-2020_11_24_390039 366 34 J. J. NNP 10_1101-2020_11_24_390039 366 35 S. S. NNP 10_1101-2020_11_24_390039 366 36 , , , 10_1101-2020_11_24_390039 366 37 Du Du NNP 10_1101-2020_11_24_390039 366 38 , , , 10_1101-2020_11_24_390039 366 39 Y. Y. NNP 10_1101-2020_11_24_390039 366 40 , , , 10_1101-2020_11_24_390039 366 41 High High NNP 10_1101-2020_11_24_390039 366 42 , , , 10_1101-2020_11_24_390039 366 43 S. S. NNP 10_1101-2020_11_24_390039 366 44 , , , 10_1101-2020_11_24_390039 366 45 et et NNP 10_1101-2020_11_24_390039 366 46 al al NNP 10_1101-2020_11_24_390039 366 47 . . . 10_1101-2020_11_24_390039 367 1 ( ( -LRB- 10_1101-2020_11_24_390039 367 2 2020 2020 CD 10_1101-2020_11_24_390039 367 3 ) ) -RRB- 10_1101-2020_11_24_390039 367 4 Ring ring NN 10_1101-2020_11_24_390039 367 5 - - HYPH 10_1101-2020_11_24_390039 367 6 expansion expansion NN 10_1101-2020_11_24_390039 367 7 leads lead VBZ 10_1101-2020_11_24_390039 367 8 to to IN 10_1101-2020_11_24_390039 367 9 a a DT 10_1101-2020_11_24_390039 367 10 more more RBR 10_1101-2020_11_24_390039 367 11 potent potent JJ 10_1101-2020_11_24_390039 367 12 analogue analogue NN 10_1101-2020_11_24_390039 367 13 of of IN 10_1101-2020_11_24_390039 367 14 ipomoeassin ipomoeassin NNP 10_1101-2020_11_24_390039 367 15 F. F. NNP 10_1101-2020_11_24_390039 367 16 J. J. NNP 10_1101-2020_11_24_390039 367 17 Org Org NNP 10_1101-2020_11_24_390039 367 18 . . . 10_1101-2020_11_24_390039 368 1 Chem Chem NNP 10_1101-2020_11_24_390039 368 2 . . . 10_1101-2020_11_24_390039 369 1 doi doi XX 10_1101-2020_11_24_390039 369 2 : : : 10_1101-2020_11_24_390039 369 3 10.1021 10.1021 CD 10_1101-2020_11_24_390039 369 4 / / SYM 10_1101-2020_11_24_390039 369 5 acs.joc.0c01659 acs.joc.0c01659 NNP 10_1101-2020_11_24_390039 369 6 .CC .CC : 10_1101-2020_11_24_390039 369 7 - - : 10_1101-2020_11_24_390039 369 8 BY by IN 10_1101-2020_11_24_390039 369 9 - - HYPH 10_1101-2020_11_24_390039 369 10 ND ND NNP 10_1101-2020_11_24_390039 369 11 4.0 4.0 CD 10_1101-2020_11_24_390039 369 12 International international JJ 10_1101-2020_11_24_390039 369 13 licensemade licensemade NN 10_1101-2020_11_24_390039 369 14 available available JJ 10_1101-2020_11_24_390039 369 15 under under IN 10_1101-2020_11_24_390039 369 16 a a DT 10_1101-2020_11_24_390039 369 17 ( ( -LRB- 10_1101-2020_11_24_390039 369 18 which which WDT 10_1101-2020_11_24_390039 369 19 was be VBD 10_1101-2020_11_24_390039 369 20 not not RB 10_1101-2020_11_24_390039 369 21 certified certify VBN 10_1101-2020_11_24_390039 369 22 by by IN 10_1101-2020_11_24_390039 369 23 peer peer NN 10_1101-2020_11_24_390039 369 24 review review NN 10_1101-2020_11_24_390039 369 25 ) ) -RRB- 10_1101-2020_11_24_390039 369 26 is be VBZ 10_1101-2020_11_24_390039 369 27 the the DT 10_1101-2020_11_24_390039 369 28 author author NN 10_1101-2020_11_24_390039 369 29 / / SYM 10_1101-2020_11_24_390039 369 30 funder funder NN 10_1101-2020_11_24_390039 369 31 , , , 10_1101-2020_11_24_390039 369 32 who who WP 10_1101-2020_11_24_390039 369 33 has have VBZ 10_1101-2020_11_24_390039 369 34 granted grant VBN 10_1101-2020_11_24_390039 369 35 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 369 36 a a DT 10_1101-2020_11_24_390039 369 37 license license NN 10_1101-2020_11_24_390039 369 38 to to TO 10_1101-2020_11_24_390039 369 39 display display VB 10_1101-2020_11_24_390039 369 40 the the DT 10_1101-2020_11_24_390039 369 41 preprint preprint NN 10_1101-2020_11_24_390039 369 42 in in IN 10_1101-2020_11_24_390039 369 43 perpetuity perpetuity NN 10_1101-2020_11_24_390039 369 44 . . . 10_1101-2020_11_24_390039 370 1 It -PRON- PRP 10_1101-2020_11_24_390039 370 2 is be VBZ 10_1101-2020_11_24_390039 370 3 The the DT 10_1101-2020_11_24_390039 370 4 copyright copyright NN 10_1101-2020_11_24_390039 370 5 holder holder NN 10_1101-2020_11_24_390039 370 6 for for IN 10_1101-2020_11_24_390039 370 7 this this DT 10_1101-2020_11_24_390039 370 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 370 9 version version NN 10_1101-2020_11_24_390039 370 10 posted post VBD 10_1101-2020_11_24_390039 370 11 January January NNP 10_1101-2020_11_24_390039 370 12 5 5 CD 10_1101-2020_11_24_390039 370 13 , , , 10_1101-2020_11_24_390039 370 14 2021 2021 CD 10_1101-2020_11_24_390039 370 15 . . . 10_1101-2020_11_24_390039 370 16 ; ; : 10_1101-2020_11_24_390039 370 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 370 18 : : : 10_1101-2020_11_24_390039 370 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 370 20 preprint preprint JJ 10_1101-2020_11_24_390039 370 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 370 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 370 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 370 24 - - HYPH 10_1101-2020_11_24_390039 370 25 F F NNP 10_1101-2020_11_24_390039 370 26 as as IN 10_1101-2020_11_24_390039 370 27 a a DT 10_1101-2020_11_24_390039 370 28 potential potential JJ 10_1101-2020_11_24_390039 370 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 370 30 agent agent NN 10_1101-2020_11_24_390039 370 31 Page Page NNP 10_1101-2020_11_24_390039 370 32 20 20 CD 10_1101-2020_11_24_390039 370 33 of of IN 10_1101-2020_11_24_390039 370 34 21 21 CD 10_1101-2020_11_24_390039 370 35 Figure Figure NNP 10_1101-2020_11_24_390039 370 36 Legends Legends NNPS 10_1101-2020_11_24_390039 370 37 Fig Fig NNP 10_1101-2020_11_24_390039 370 38 . . . 10_1101-2020_11_24_390039 371 1 1 1 LS 10_1101-2020_11_24_390039 371 2 . . . 10_1101-2020_11_24_390039 372 1 Ipom Ipom NNP 10_1101-2020_11_24_390039 372 2 - - HYPH 10_1101-2020_11_24_390039 372 3 F F NNP 10_1101-2020_11_24_390039 372 4 as as IN 10_1101-2020_11_24_390039 372 5 a a DT 10_1101-2020_11_24_390039 372 6 potential potential JJ 10_1101-2020_11_24_390039 372 7 inhibitor inhibitor NN 10_1101-2020_11_24_390039 372 8 of of IN 10_1101-2020_11_24_390039 372 9 SARS SARS NNP 10_1101-2020_11_24_390039 372 10 - - HYPH 10_1101-2020_11_24_390039 372 11 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 372 12 viral viral JJ 10_1101-2020_11_24_390039 372 13 protein protein NN 10_1101-2020_11_24_390039 372 14 synthesis synthesis NN 10_1101-2020_11_24_390039 372 15 . . . 10_1101-2020_11_24_390039 373 1 ( ( -LRB- 10_1101-2020_11_24_390039 373 2 A a DT 10_1101-2020_11_24_390039 373 3 ) ) -RRB- 10_1101-2020_11_24_390039 373 4 Schematic schematic JJ 10_1101-2020_11_24_390039 373 5 of of IN 10_1101-2020_11_24_390039 373 6 ( ( -LRB- 10_1101-2020_11_24_390039 373 7 + + SYM 10_1101-2020_11_24_390039 373 8 ) ) -RRB- 10_1101-2020_11_24_390039 373 9 ssRNA ssRNA NFP 10_1101-2020_11_24_390039 373 10 genome genome JJ 10_1101-2020_11_24_390039 373 11 architecture architecture NN 10_1101-2020_11_24_390039 373 12 of of IN 10_1101-2020_11_24_390039 373 13 SARS SARS NNP 10_1101-2020_11_24_390039 373 14 - - HYPH 10_1101-2020_11_24_390039 373 15 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 373 16 ( ( -LRB- 10_1101-2020_11_24_390039 373 17 29903 29903 CD 10_1101-2020_11_24_390039 373 18 nt nt RB 10_1101-2020_11_24_390039 373 19 ) ) -RRB- 10_1101-2020_11_24_390039 373 20 containing contain VBG 10_1101-2020_11_24_390039 373 21 5 5 CD 10_1101-2020_11_24_390039 373 22 ’ ' '' 10_1101-2020_11_24_390039 373 23 capped capped JJ 10_1101-2020_11_24_390039 373 24 mRNA mrna NN 10_1101-2020_11_24_390039 373 25 with with IN 10_1101-2020_11_24_390039 373 26 a a DT 10_1101-2020_11_24_390039 373 27 leader leader NN 10_1101-2020_11_24_390039 373 28 sequence sequence NN 10_1101-2020_11_24_390039 373 29 ( ( -LRB- 10_1101-2020_11_24_390039 373 30 LS LS NNP 10_1101-2020_11_24_390039 373 31 ) ) -RRB- 10_1101-2020_11_24_390039 373 32 , , , 10_1101-2020_11_24_390039 373 33 3 3 CD 10_1101-2020_11_24_390039 373 34 ’ ' '' 10_1101-2020_11_24_390039 373 35 end end NN 10_1101-2020_11_24_390039 373 36 poly poly NN 10_1101-2020_11_24_390039 373 37 - - , 10_1101-2020_11_24_390039 373 38 A a NN 10_1101-2020_11_24_390039 373 39 tail tail NN 10_1101-2020_11_24_390039 373 40 , , , 10_1101-2020_11_24_390039 373 41 5 5 CD 10_1101-2020_11_24_390039 373 42 ’ ' '' 10_1101-2020_11_24_390039 373 43 and and CC 10_1101-2020_11_24_390039 373 44 3 3 CD 10_1101-2020_11_24_390039 373 45 ’ ' '' 10_1101-2020_11_24_390039 373 46 UTRs utr NNS 10_1101-2020_11_24_390039 373 47 and and CC 10_1101-2020_11_24_390039 373 48 open open JJ 10_1101-2020_11_24_390039 373 49 reading reading NN 10_1101-2020_11_24_390039 373 50 frames frame NNS 10_1101-2020_11_24_390039 373 51 ( ( -LRB- 10_1101-2020_11_24_390039 373 52 ORFs ORFs NNP 10_1101-2020_11_24_390039 373 53 ) ) -RRB- 10_1101-2020_11_24_390039 373 54 : : : 10_1101-2020_11_24_390039 373 55 ORF1a ORF1a NNP 10_1101-2020_11_24_390039 373 56 , , , 10_1101-2020_11_24_390039 373 57 ORF1b ORF1b NNP 10_1101-2020_11_24_390039 373 58 , , , 10_1101-2020_11_24_390039 373 59 spike spike NNP 10_1101-2020_11_24_390039 373 60 ( ( -LRB- 10_1101-2020_11_24_390039 373 61 S S NNP 10_1101-2020_11_24_390039 373 62 ) ) -RRB- 10_1101-2020_11_24_390039 373 63 , , , 10_1101-2020_11_24_390039 373 64 ORF3a ORF3a NNP 10_1101-2020_11_24_390039 373 65 , , , 10_1101-2020_11_24_390039 373 66 envelope envelope NNP 10_1101-2020_11_24_390039 373 67 ( ( -LRB- 10_1101-2020_11_24_390039 373 68 E E NNP 10_1101-2020_11_24_390039 373 69 ) ) -RRB- 10_1101-2020_11_24_390039 373 70 , , , 10_1101-2020_11_24_390039 373 71 membrane membrane NN 10_1101-2020_11_24_390039 373 72 ( ( -LRB- 10_1101-2020_11_24_390039 373 73 M M NNP 10_1101-2020_11_24_390039 373 74 ) ) -RRB- 10_1101-2020_11_24_390039 373 75 , , , 10_1101-2020_11_24_390039 373 76 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 373 77 , , , 10_1101-2020_11_24_390039 373 78 ORF7 ORF7 NNP 10_1101-2020_11_24_390039 373 79 , , , 10_1101-2020_11_24_390039 373 80 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 373 81 , , , 10_1101-2020_11_24_390039 373 82 nucleoprotein nucleoprotein NNP 10_1101-2020_11_24_390039 373 83 ( ( -LRB- 10_1101-2020_11_24_390039 373 84 N n NN 10_1101-2020_11_24_390039 373 85 ) ) -RRB- 10_1101-2020_11_24_390039 373 86 and and CC 10_1101-2020_11_24_390039 373 87 ORF10 orf10 NN 10_1101-2020_11_24_390039 373 88 ( ( -LRB- 10_1101-2020_11_24_390039 373 89 Firth firth NN 10_1101-2020_11_24_390039 373 90 , , , 10_1101-2020_11_24_390039 373 91 2020 2020 CD 10_1101-2020_11_24_390039 373 92 ; ; : 10_1101-2020_11_24_390039 373 93 Naqvi Naqvi NNP 10_1101-2020_11_24_390039 373 94 et et NNP 10_1101-2020_11_24_390039 373 95 al al NNP 10_1101-2020_11_24_390039 373 96 . . NNP 10_1101-2020_11_24_390039 373 97 , , , 10_1101-2020_11_24_390039 373 98 2020 2020 CD 10_1101-2020_11_24_390039 373 99 ) ) -RRB- 10_1101-2020_11_24_390039 373 100 . . . 10_1101-2020_11_24_390039 374 1 An an DT 10_1101-2020_11_24_390039 374 2 important important JJ 10_1101-2020_11_24_390039 374 3 mode mode NN 10_1101-2020_11_24_390039 374 4 of of IN 10_1101-2020_11_24_390039 374 5 SARS SARS NNP 10_1101-2020_11_24_390039 374 6 - - HYPH 10_1101-2020_11_24_390039 374 7 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 374 8 host host NN 10_1101-2020_11_24_390039 374 9 entry entry NN 10_1101-2020_11_24_390039 374 10 proceeds proceed VBZ 10_1101-2020_11_24_390039 374 11 via via IN 10_1101-2020_11_24_390039 374 12 interaction interaction NN 10_1101-2020_11_24_390039 374 13 of of IN 10_1101-2020_11_24_390039 374 14 the the DT 10_1101-2020_11_24_390039 374 15 viral viral JJ 10_1101-2020_11_24_390039 374 16 S S NNP 10_1101-2020_11_24_390039 374 17 protein protein NN 10_1101-2020_11_24_390039 374 18 with with IN 10_1101-2020_11_24_390039 374 19 human human NN 10_1101-2020_11_24_390039 374 20 angiotensin- angiotensin- JJ 10_1101-2020_11_24_390039 374 21 converting convert VBG 10_1101-2020_11_24_390039 374 22 enzyme enzyme NNS 10_1101-2020_11_24_390039 374 23 2 2 CD 10_1101-2020_11_24_390039 374 24 ( ( -LRB- 10_1101-2020_11_24_390039 374 25 ACE2 ACE2 NNP 10_1101-2020_11_24_390039 374 26 ) ) -RRB- 10_1101-2020_11_24_390039 374 27 ( ( -LRB- 10_1101-2020_11_24_390039 374 28 Walls Walls NNP 10_1101-2020_11_24_390039 374 29 et et FW 10_1101-2020_11_24_390039 374 30 al al NNP 10_1101-2020_11_24_390039 374 31 . . NNP 10_1101-2020_11_24_390039 374 32 , , , 10_1101-2020_11_24_390039 374 33 2020 2020 CD 10_1101-2020_11_24_390039 374 34 ) ) -RRB- 10_1101-2020_11_24_390039 374 35 . . . 10_1101-2020_11_24_390039 375 1 ( ( -LRB- 10_1101-2020_11_24_390039 375 2 B b NN 10_1101-2020_11_24_390039 375 3 ) ) -RRB- 10_1101-2020_11_24_390039 375 4 Structure structure NN 10_1101-2020_11_24_390039 375 5 of of IN 10_1101-2020_11_24_390039 375 6 Ipomoeassin- Ipomoeassin- NNP 10_1101-2020_11_24_390039 375 7 F F NNP 10_1101-2020_11_24_390039 375 8 ( ( -LRB- 10_1101-2020_11_24_390039 375 9 Ipom Ipom NNP 10_1101-2020_11_24_390039 375 10 - - HYPH 10_1101-2020_11_24_390039 375 11 F F NNP 10_1101-2020_11_24_390039 375 12 ) ) -RRB- 10_1101-2020_11_24_390039 375 13 , , , 10_1101-2020_11_24_390039 375 14 a a DT 10_1101-2020_11_24_390039 375 15 small small JJ 10_1101-2020_11_24_390039 375 16 molecule molecule JJ 10_1101-2020_11_24_390039 375 17 inhibitor inhibitor NN 10_1101-2020_11_24_390039 375 18 of of IN 10_1101-2020_11_24_390039 375 19 Sec61-mediated sec61-mediate VBN 10_1101-2020_11_24_390039 375 20 protein protein NN 10_1101-2020_11_24_390039 375 21 translocation translocation NN 10_1101-2020_11_24_390039 375 22 . . . 10_1101-2020_11_24_390039 376 1 ( ( -LRB- 10_1101-2020_11_24_390039 376 2 C c NN 10_1101-2020_11_24_390039 376 3 ) ) -RRB- 10_1101-2020_11_24_390039 376 4 Ipom Ipom NNP 10_1101-2020_11_24_390039 376 5 - - HYPH 10_1101-2020_11_24_390039 376 6 F F NNP 10_1101-2020_11_24_390039 376 7 efficiently efficiently RB 10_1101-2020_11_24_390039 376 8 blocks block VBZ 10_1101-2020_11_24_390039 376 9 membrane membrane NN 10_1101-2020_11_24_390039 376 10 translocation translocation NN 10_1101-2020_11_24_390039 376 11 of of IN 10_1101-2020_11_24_390039 376 12 secretory secretory JJ 10_1101-2020_11_24_390039 376 13 proteins protein NNS 10_1101-2020_11_24_390039 376 14 and and CC 10_1101-2020_11_24_390039 376 15 insertion insertion NN 10_1101-2020_11_24_390039 376 16 of of IN 10_1101-2020_11_24_390039 376 17 single single JJ 10_1101-2020_11_24_390039 376 18 - - HYPH 10_1101-2020_11_24_390039 376 19 pass pass NN 10_1101-2020_11_24_390039 376 20 type type NN 10_1101-2020_11_24_390039 376 21 I -PRON- PRP 10_1101-2020_11_24_390039 376 22 and and CC 10_1101-2020_11_24_390039 376 23 type type VB 10_1101-2020_11_24_390039 376 24 II II NNP 10_1101-2020_11_24_390039 376 25 TMPs tmp NNS 10_1101-2020_11_24_390039 376 26 , , , 10_1101-2020_11_24_390039 376 27 but but CC 10_1101-2020_11_24_390039 376 28 not not RB 10_1101-2020_11_24_390039 376 29 insertion insertion NN 10_1101-2020_11_24_390039 376 30 of of IN 10_1101-2020_11_24_390039 376 31 type type NN 10_1101-2020_11_24_390039 376 32 III iii CD 10_1101-2020_11_24_390039 376 33 TMPs tmp NNS 10_1101-2020_11_24_390039 376 34 or or CC 10_1101-2020_11_24_390039 376 35 tail tail NN 10_1101-2020_11_24_390039 376 36 - - HYPH 10_1101-2020_11_24_390039 376 37 anchored anchor VBN 10_1101-2020_11_24_390039 376 38 ( ( -LRB- 10_1101-2020_11_24_390039 376 39 TA TA NNP 10_1101-2020_11_24_390039 376 40 ) ) -RRB- 10_1101-2020_11_24_390039 376 41 proteins protein NNS 10_1101-2020_11_24_390039 376 42 . . . 10_1101-2020_11_24_390039 377 1 SA SA NNP 10_1101-2020_11_24_390039 377 2 denotes denote VBZ 10_1101-2020_11_24_390039 377 3 a a DT 10_1101-2020_11_24_390039 377 4 signal signal NN 10_1101-2020_11_24_390039 377 5 anchor anchor NN 10_1101-2020_11_24_390039 377 6 . . . 10_1101-2020_11_24_390039 378 1 ( ( -LRB- 10_1101-2020_11_24_390039 378 2 D d NN 10_1101-2020_11_24_390039 378 3 ) ) -RRB- 10_1101-2020_11_24_390039 378 4 Based base VBN 10_1101-2020_11_24_390039 378 5 on on IN 10_1101-2020_11_24_390039 378 6 known know VBN 10_1101-2020_11_24_390039 378 7 / / SYM 10_1101-2020_11_24_390039 378 8 predicted predict VBN 10_1101-2020_11_24_390039 378 9 membrane membrane NN 10_1101-2020_11_24_390039 378 10 topology topology NN 10_1101-2020_11_24_390039 378 11 of of IN 10_1101-2020_11_24_390039 378 12 SARS SARS NNP 10_1101-2020_11_24_390039 378 13 - - HYPH 10_1101-2020_11_24_390039 378 14 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 378 15 proteins protein NNS 10_1101-2020_11_24_390039 378 16 , , , 10_1101-2020_11_24_390039 378 17 and and CC 10_1101-2020_11_24_390039 378 18 sensitivity sensitivity NN 10_1101-2020_11_24_390039 378 19 of of IN 10_1101-2020_11_24_390039 378 20 comparable comparable JJ 10_1101-2020_11_24_390039 378 21 host host NN 10_1101-2020_11_24_390039 378 22 cell cell NN 10_1101-2020_11_24_390039 378 23 proteins protein NNS 10_1101-2020_11_24_390039 378 24 ( ( -LRB- 10_1101-2020_11_24_390039 378 25 Zong Zong NNP 10_1101-2020_11_24_390039 378 26 et et NNP 10_1101-2020_11_24_390039 378 27 al al NNP 10_1101-2020_11_24_390039 378 28 . . NNP 10_1101-2020_11_24_390039 378 29 , , , 10_1101-2020_11_24_390039 378 30 2019 2019 CD 10_1101-2020_11_24_390039 378 31 ; ; : 10_1101-2020_11_24_390039 378 32 O’Keefe O’Keefe NNP 10_1101-2020_11_24_390039 378 33 et et NNP 10_1101-2020_11_24_390039 378 34 al al NNP 10_1101-2020_11_24_390039 378 35 . . NNP 10_1101-2020_11_24_390039 378 36 , , , 10_1101-2020_11_24_390039 378 37 2020 2020 CD 10_1101-2020_11_24_390039 378 38 submitted submit VBN 10_1101-2020_11_24_390039 378 39 ) ) -RRB- 10_1101-2020_11_24_390039 378 40 , , , 10_1101-2020_11_24_390039 378 41 likely likely JJ 10_1101-2020_11_24_390039 378 42 sensitivity sensitivity NN 10_1101-2020_11_24_390039 378 43 to to IN 10_1101-2020_11_24_390039 378 44 Ipom Ipom NNP 10_1101-2020_11_24_390039 378 45 - - HYPH 10_1101-2020_11_24_390039 378 46 F F NNP 10_1101-2020_11_24_390039 378 47 was be VBD 10_1101-2020_11_24_390039 378 48 anticipated anticipate VBN 10_1101-2020_11_24_390039 378 49 . . . 10_1101-2020_11_24_390039 379 1 Fig fig NN 10_1101-2020_11_24_390039 379 2 . . . 10_1101-2020_11_24_390039 380 1 2 2 LS 10_1101-2020_11_24_390039 380 2 . . . 10_1101-2020_11_24_390039 381 1 Ipom Ipom NNP 10_1101-2020_11_24_390039 381 2 - - HYPH 10_1101-2020_11_24_390039 381 3 F F NNP 10_1101-2020_11_24_390039 381 4 selectively selectively RB 10_1101-2020_11_24_390039 381 5 inhibits inhibit VBZ 10_1101-2020_11_24_390039 381 6 the the DT 10_1101-2020_11_24_390039 381 7 ER ER NNP 10_1101-2020_11_24_390039 381 8 membrane membrane NN 10_1101-2020_11_24_390039 381 9 translocation translocation NN 10_1101-2020_11_24_390039 381 10 of of IN 10_1101-2020_11_24_390039 381 11 SARS- SARS- NNP 10_1101-2020_11_24_390039 381 12 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 381 13 proteins protein NNS 10_1101-2020_11_24_390039 381 14 . . . 10_1101-2020_11_24_390039 382 1 ( ( -LRB- 10_1101-2020_11_24_390039 382 2 A a DT 10_1101-2020_11_24_390039 382 3 ) ) -RRB- 10_1101-2020_11_24_390039 382 4 Schematic schematic JJ 10_1101-2020_11_24_390039 382 5 of of IN 10_1101-2020_11_24_390039 382 6 in in FW 10_1101-2020_11_24_390039 382 7 vitro vitro FW 10_1101-2020_11_24_390039 382 8 ER ER NNP 10_1101-2020_11_24_390039 382 9 import import NN 10_1101-2020_11_24_390039 382 10 assay assay NN 10_1101-2020_11_24_390039 382 11 using use VBG 10_1101-2020_11_24_390039 382 12 pancreatic pancreatic JJ 10_1101-2020_11_24_390039 382 13 microsomes microsome NNS 10_1101-2020_11_24_390039 382 14 . . . 10_1101-2020_11_24_390039 383 1 Following follow VBG 10_1101-2020_11_24_390039 383 2 translation translation NN 10_1101-2020_11_24_390039 383 3 , , , 10_1101-2020_11_24_390039 383 4 fully fully RB 10_1101-2020_11_24_390039 383 5 translocated translocate VBN 10_1101-2020_11_24_390039 383 6 / / SYM 10_1101-2020_11_24_390039 383 7 membrane membrane NN 10_1101-2020_11_24_390039 383 8 inserted insert VBD 10_1101-2020_11_24_390039 383 9 radiolabelled radiolabelle VBN 10_1101-2020_11_24_390039 383 10 precursor precursor NN 10_1101-2020_11_24_390039 383 11 proteins protein NNS 10_1101-2020_11_24_390039 383 12 are be VBP 10_1101-2020_11_24_390039 383 13 recovered recover VBN 10_1101-2020_11_24_390039 383 14 and and CC 10_1101-2020_11_24_390039 383 15 analysed analyse VBN 10_1101-2020_11_24_390039 383 16 by by IN 10_1101-2020_11_24_390039 383 17 SDS SDS NNP 10_1101-2020_11_24_390039 383 18 - - HYPH 10_1101-2020_11_24_390039 383 19 PAGE page NN 10_1101-2020_11_24_390039 383 20 and and CC 10_1101-2020_11_24_390039 383 21 phosphorimaging phosphorimaging NN 10_1101-2020_11_24_390039 383 22 . . . 10_1101-2020_11_24_390039 384 1 N n LS 10_1101-2020_11_24_390039 384 2 - - HYPH 10_1101-2020_11_24_390039 384 3 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 384 4 species specie NNS 10_1101-2020_11_24_390039 384 5 were be VBD 10_1101-2020_11_24_390039 384 6 confirmed confirm VBN 10_1101-2020_11_24_390039 384 7 by by IN 10_1101-2020_11_24_390039 384 8 treatment treatment NN 10_1101-2020_11_24_390039 384 9 with with IN 10_1101-2020_11_24_390039 384 10 endoglycosidase endoglycosidase NN 10_1101-2020_11_24_390039 384 11 H h NN 10_1101-2020_11_24_390039 384 12 ( ( -LRB- 10_1101-2020_11_24_390039 384 13 Endo Endo NNP 10_1101-2020_11_24_390039 384 14 H H NNP 10_1101-2020_11_24_390039 384 15 ) ) -RRB- 10_1101-2020_11_24_390039 384 16 . . . 10_1101-2020_11_24_390039 385 1 ( ( -LRB- 10_1101-2020_11_24_390039 385 2 B b NN 10_1101-2020_11_24_390039 385 3 ) ) -RRB- 10_1101-2020_11_24_390039 385 4 Protein protein NN 10_1101-2020_11_24_390039 385 5 precursors precursor NNS 10_1101-2020_11_24_390039 385 6 of of IN 10_1101-2020_11_24_390039 385 7 the the DT 10_1101-2020_11_24_390039 385 8 human human NN 10_1101-2020_11_24_390039 385 9 angiotensin- angiotensin- JJ 10_1101-2020_11_24_390039 385 10 converting convert VBG 10_1101-2020_11_24_390039 385 11 enzyme enzyme NNS 10_1101-2020_11_24_390039 385 12 2 2 CD 10_1101-2020_11_24_390039 385 13 ( ( -LRB- 10_1101-2020_11_24_390039 385 14 ACE2 ACE2 NNP 10_1101-2020_11_24_390039 385 15 ) ) -RRB- 10_1101-2020_11_24_390039 385 16 and and CC 10_1101-2020_11_24_390039 385 17 OPG2-tagged opg2-tagged JJ 10_1101-2020_11_24_390039 385 18 versions version NNS 10_1101-2020_11_24_390039 385 19 of of IN 10_1101-2020_11_24_390039 385 20 the the DT 10_1101-2020_11_24_390039 385 21 SARS SARS NNP 10_1101-2020_11_24_390039 385 22 - - HYPH 10_1101-2020_11_24_390039 385 23 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 385 24 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 385 25 ( ( -LRB- 10_1101-2020_11_24_390039 385 26 ORF8-OPG2 ORF8-OPG2 NNP 10_1101-2020_11_24_390039 385 27 ) ) -RRB- 10_1101-2020_11_24_390039 385 28 , , , 10_1101-2020_11_24_390039 385 29 spike spike NNP 10_1101-2020_11_24_390039 385 30 ( ( -LRB- 10_1101-2020_11_24_390039 385 31 S S NNP 10_1101-2020_11_24_390039 385 32 - - HYPH 10_1101-2020_11_24_390039 385 33 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 385 34 ) ) -RRB- 10_1101-2020_11_24_390039 385 35 , , , 10_1101-2020_11_24_390039 385 36 envelope envelope NNP 10_1101-2020_11_24_390039 385 37 ( ( -LRB- 10_1101-2020_11_24_390039 385 38 OPG2-E OPG2-E NNP 10_1101-2020_11_24_390039 385 39 ) ) -RRB- 10_1101-2020_11_24_390039 385 40 , , , 10_1101-2020_11_24_390039 385 41 membrane membrane NN 10_1101-2020_11_24_390039 385 42 ( ( -LRB- 10_1101-2020_11_24_390039 385 43 M- M- NNP 10_1101-2020_11_24_390039 385 44 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 385 45 ) ) -RRB- 10_1101-2020_11_24_390039 385 46 and and CC 10_1101-2020_11_24_390039 385 47 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 385 48 ( ( -LRB- 10_1101-2020_11_24_390039 385 49 a a DT 10_1101-2020_11_24_390039 385 50 doubly doubly RB 10_1101-2020_11_24_390039 385 51 - - HYPH 10_1101-2020_11_24_390039 385 52 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 385 53 tagged tag VBN 10_1101-2020_11_24_390039 385 54 version version NN 10_1101-2020_11_24_390039 385 55 , , , 10_1101-2020_11_24_390039 385 56 OPG2-ORF6-OPG2 OPG2-ORF6-OPG2 NNP 10_1101-2020_11_24_390039 385 57 , , , 10_1101-2020_11_24_390039 385 58 and and CC 10_1101-2020_11_24_390039 385 59 two two CD 10_1101-2020_11_24_390039 385 60 singly singly RB 10_1101-2020_11_24_390039 385 61 - - HYPH 10_1101-2020_11_24_390039 385 62 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 385 63 tagged tag VBN 10_1101-2020_11_24_390039 385 64 forms form NNS 10_1101-2020_11_24_390039 385 65 , , , 10_1101-2020_11_24_390039 385 66 OPG2-ORF6 OPG2-ORF6 NNP 10_1101-2020_11_24_390039 385 67 and and CC 10_1101-2020_11_24_390039 385 68 ORF6-OPG2 ORF6-OPG2 NNP 10_1101-2020_11_24_390039 385 69 , , , 10_1101-2020_11_24_390039 385 70 with with IN 10_1101-2020_11_24_390039 385 71 predominant predominant NNP 10_1101-2020_11_24_390039 385 72 N- n- CD 10_1101-2020_11_24_390039 385 73 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 385 74 species specie NNS 10_1101-2020_11_24_390039 385 75 in in IN 10_1101-2020_11_24_390039 385 76 bold bold NNP 10_1101-2020_11_24_390039 385 77 ) ) -RRB- 10_1101-2020_11_24_390039 385 78 were be VBD 10_1101-2020_11_24_390039 385 79 synthesised synthesise VBN 10_1101-2020_11_24_390039 385 80 in in IN 10_1101-2020_11_24_390039 385 81 rabbit rabbit NN 10_1101-2020_11_24_390039 385 82 reticulocyte reticulocyte NNP 10_1101-2020_11_24_390039 385 83 lysate lysate NN 10_1101-2020_11_24_390039 385 84 supplemented supplement VBN 10_1101-2020_11_24_390039 385 85 with with IN 10_1101-2020_11_24_390039 385 86 ER ER NNP 10_1101-2020_11_24_390039 385 87 microsomes microsome NNS 10_1101-2020_11_24_390039 385 88 without without IN 10_1101-2020_11_24_390039 385 89 or or CC 10_1101-2020_11_24_390039 385 90 with with IN 10_1101-2020_11_24_390039 385 91 Ipom Ipom NNP 10_1101-2020_11_24_390039 385 92 - - HYPH 10_1101-2020_11_24_390039 385 93 F F NNP 10_1101-2020_11_24_390039 385 94 ( ( -LRB- 10_1101-2020_11_24_390039 385 95 lanes lanes NNP 10_1101-2020_11_24_390039 385 96 1 1 CD 10_1101-2020_11_24_390039 385 97 and and CC 10_1101-2020_11_24_390039 385 98 3 3 CD 10_1101-2020_11_24_390039 385 99 ) ) -RRB- 10_1101-2020_11_24_390039 385 100 . . . 10_1101-2020_11_24_390039 386 1 Phosphorimages phosphorimage NNS 10_1101-2020_11_24_390039 386 2 of of IN 10_1101-2020_11_24_390039 386 3 membrane membrane NN 10_1101-2020_11_24_390039 386 4 - - HYPH 10_1101-2020_11_24_390039 386 5 associated associate VBN 10_1101-2020_11_24_390039 386 6 products product NNS 10_1101-2020_11_24_390039 386 7 resolved resolve VBN 10_1101-2020_11_24_390039 386 8 by by IN 10_1101-2020_11_24_390039 386 9 SDS SDS NNP 10_1101-2020_11_24_390039 386 10 - - HYPH 10_1101-2020_11_24_390039 386 11 PAGE PAGE NNP 10_1101-2020_11_24_390039 386 12 with with IN 10_1101-2020_11_24_390039 386 13 representative representative JJ 10_1101-2020_11_24_390039 386 14 substrate substrate NN 10_1101-2020_11_24_390039 386 15 outlines outline NNS 10_1101-2020_11_24_390039 386 16 are be VBP 10_1101-2020_11_24_390039 386 17 shown show VBN 10_1101-2020_11_24_390039 386 18 . . . 10_1101-2020_11_24_390039 387 1 N n NN 10_1101-2020_11_24_390039 387 2 - - HYPH 10_1101-2020_11_24_390039 387 3 glycosylation glycosylation NN 10_1101-2020_11_24_390039 387 4 was be VBD 10_1101-2020_11_24_390039 387 5 used use VBN 10_1101-2020_11_24_390039 387 6 to to TO 10_1101-2020_11_24_390039 387 7 measure measure VB 10_1101-2020_11_24_390039 387 8 the the DT 10_1101-2020_11_24_390039 387 9 efficiency efficiency NN 10_1101-2020_11_24_390039 387 10 of of IN 10_1101-2020_11_24_390039 387 11 membrane membrane NN 10_1101-2020_11_24_390039 387 12 translocation translocation NN 10_1101-2020_11_24_390039 387 13 / / SYM 10_1101-2020_11_24_390039 387 14 insertion insertion NN 10_1101-2020_11_24_390039 387 15 and and CC 10_1101-2020_11_24_390039 387 16 N N NNP 10_1101-2020_11_24_390039 387 17 - - HYPH 10_1101-2020_11_24_390039 387 18 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 387 19 ( ( -LRB- 10_1101-2020_11_24_390039 387 20 X X NNP 10_1101-2020_11_24_390039 387 21 - - NNP 10_1101-2020_11_24_390039 387 22 Gly gly NN 10_1101-2020_11_24_390039 387 23 ) ) -RRB- 10_1101-2020_11_24_390039 387 24 versus versus IN 10_1101-2020_11_24_390039 387 25 non non JJ 10_1101-2020_11_24_390039 387 26 - - JJ 10_1101-2020_11_24_390039 387 27 N n JJ 10_1101-2020_11_24_390039 387 28 - - HYPH 10_1101-2020_11_24_390039 387 29 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 387 30 ( ( -LRB- 10_1101-2020_11_24_390039 387 31 0Gly 0gly NN 10_1101-2020_11_24_390039 387 32 ) ) -RRB- 10_1101-2020_11_24_390039 387 33 species specie NNS 10_1101-2020_11_24_390039 387 34 identified identify VBN 10_1101-2020_11_24_390039 387 35 using use VBG 10_1101-2020_11_24_390039 387 36 Endo Endo NNP 10_1101-2020_11_24_390039 387 37 H H NNP 10_1101-2020_11_24_390039 387 38 ( ( -LRB- 10_1101-2020_11_24_390039 387 39 see see VB 10_1101-2020_11_24_390039 387 40 lane lane NNP 10_1101-2020_11_24_390039 387 41 2 2 CD 10_1101-2020_11_24_390039 387 42 ) ) -RRB- 10_1101-2020_11_24_390039 387 43 . . . 10_1101-2020_11_24_390039 388 1 ( ( -LRB- 10_1101-2020_11_24_390039 388 2 C C NNP 10_1101-2020_11_24_390039 388 3 ) ) -RRB- 10_1101-2020_11_24_390039 388 4 The the DT 10_1101-2020_11_24_390039 388 5 relative relative JJ 10_1101-2020_11_24_390039 388 6 efficiency efficiency NN 10_1101-2020_11_24_390039 388 7 of of IN 10_1101-2020_11_24_390039 388 8 membrane membrane NN 10_1101-2020_11_24_390039 388 9 translocation translocation NN 10_1101-2020_11_24_390039 388 10 / / SYM 10_1101-2020_11_24_390039 388 11 insertion insertion NN 10_1101-2020_11_24_390039 388 12 in in IN 10_1101-2020_11_24_390039 388 13 the the DT 10_1101-2020_11_24_390039 388 14 .CC .CC : 10_1101-2020_11_24_390039 388 15 - - HYPH 10_1101-2020_11_24_390039 388 16 BY by IN 10_1101-2020_11_24_390039 388 17 - - HYPH 10_1101-2020_11_24_390039 388 18 ND ND NNP 10_1101-2020_11_24_390039 388 19 4.0 4.0 CD 10_1101-2020_11_24_390039 388 20 International international JJ 10_1101-2020_11_24_390039 388 21 licensemade licensemade NN 10_1101-2020_11_24_390039 388 22 available available JJ 10_1101-2020_11_24_390039 388 23 under under IN 10_1101-2020_11_24_390039 388 24 a a DT 10_1101-2020_11_24_390039 388 25 ( ( -LRB- 10_1101-2020_11_24_390039 388 26 which which WDT 10_1101-2020_11_24_390039 388 27 was be VBD 10_1101-2020_11_24_390039 388 28 not not RB 10_1101-2020_11_24_390039 388 29 certified certify VBN 10_1101-2020_11_24_390039 388 30 by by IN 10_1101-2020_11_24_390039 388 31 peer peer NN 10_1101-2020_11_24_390039 388 32 review review NN 10_1101-2020_11_24_390039 388 33 ) ) -RRB- 10_1101-2020_11_24_390039 388 34 is be VBZ 10_1101-2020_11_24_390039 388 35 the the DT 10_1101-2020_11_24_390039 388 36 author author NN 10_1101-2020_11_24_390039 388 37 / / SYM 10_1101-2020_11_24_390039 388 38 funder funder NN 10_1101-2020_11_24_390039 388 39 , , , 10_1101-2020_11_24_390039 388 40 who who WP 10_1101-2020_11_24_390039 388 41 has have VBZ 10_1101-2020_11_24_390039 388 42 granted grant VBN 10_1101-2020_11_24_390039 388 43 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 388 44 a a DT 10_1101-2020_11_24_390039 388 45 license license NN 10_1101-2020_11_24_390039 388 46 to to TO 10_1101-2020_11_24_390039 388 47 display display VB 10_1101-2020_11_24_390039 388 48 the the DT 10_1101-2020_11_24_390039 388 49 preprint preprint NN 10_1101-2020_11_24_390039 388 50 in in IN 10_1101-2020_11_24_390039 388 51 perpetuity perpetuity NN 10_1101-2020_11_24_390039 388 52 . . . 10_1101-2020_11_24_390039 389 1 It -PRON- PRP 10_1101-2020_11_24_390039 389 2 is be VBZ 10_1101-2020_11_24_390039 389 3 The the DT 10_1101-2020_11_24_390039 389 4 copyright copyright NN 10_1101-2020_11_24_390039 389 5 holder holder NN 10_1101-2020_11_24_390039 389 6 for for IN 10_1101-2020_11_24_390039 389 7 this this DT 10_1101-2020_11_24_390039 389 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 389 9 version version NN 10_1101-2020_11_24_390039 389 10 posted post VBD 10_1101-2020_11_24_390039 389 11 January January NNP 10_1101-2020_11_24_390039 389 12 5 5 CD 10_1101-2020_11_24_390039 389 13 , , , 10_1101-2020_11_24_390039 389 14 2021 2021 CD 10_1101-2020_11_24_390039 389 15 . . . 10_1101-2020_11_24_390039 389 16 ; ; : 10_1101-2020_11_24_390039 389 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 389 18 : : : 10_1101-2020_11_24_390039 389 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 389 20 preprint preprint JJ 10_1101-2020_11_24_390039 389 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 389 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 389 23 Ipom Ipom NNP 10_1101-2020_11_24_390039 389 24 - - HYPH 10_1101-2020_11_24_390039 389 25 F F NNP 10_1101-2020_11_24_390039 389 26 as as IN 10_1101-2020_11_24_390039 389 27 a a DT 10_1101-2020_11_24_390039 389 28 potential potential JJ 10_1101-2020_11_24_390039 389 29 antiviral antiviral JJ 10_1101-2020_11_24_390039 389 30 agent agent NN 10_1101-2020_11_24_390039 389 31 Page Page NNP 10_1101-2020_11_24_390039 389 32 21 21 CD 10_1101-2020_11_24_390039 389 33 of of IN 10_1101-2020_11_24_390039 389 34 21 21 CD 10_1101-2020_11_24_390039 389 35 presence presence NN 10_1101-2020_11_24_390039 389 36 of of IN 10_1101-2020_11_24_390039 389 37 Ipom Ipom NNP 10_1101-2020_11_24_390039 389 38 - - HYPH 10_1101-2020_11_24_390039 389 39 F F NNP 10_1101-2020_11_24_390039 389 40 was be VBD 10_1101-2020_11_24_390039 389 41 calculated calculate VBN 10_1101-2020_11_24_390039 389 42 using use VBG 10_1101-2020_11_24_390039 389 43 the the DT 10_1101-2020_11_24_390039 389 44 ratio ratio NN 10_1101-2020_11_24_390039 389 45 of of IN 10_1101-2020_11_24_390039 389 46 N n NN 10_1101-2020_11_24_390039 389 47 - - HYPH 10_1101-2020_11_24_390039 389 48 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 389 49 protein protein NN 10_1101-2020_11_24_390039 389 50 to to IN 10_1101-2020_11_24_390039 389 51 non non JJ 10_1101-2020_11_24_390039 389 52 - - JJ 10_1101-2020_11_24_390039 389 53 glycosylated glycosylated JJ 10_1101-2020_11_24_390039 389 54 protein protein NN 10_1101-2020_11_24_390039 389 55 , , , 10_1101-2020_11_24_390039 389 56 relative relative JJ 10_1101-2020_11_24_390039 389 57 to to IN 10_1101-2020_11_24_390039 389 58 the the DT 10_1101-2020_11_24_390039 389 59 DMSO DMSO NNP 10_1101-2020_11_24_390039 389 60 treated treat VBN 10_1101-2020_11_24_390039 389 61 control control NN 10_1101-2020_11_24_390039 389 62 ( ( -LRB- 10_1101-2020_11_24_390039 389 63 set set VBN 10_1101-2020_11_24_390039 389 64 to to IN 10_1101-2020_11_24_390039 389 65 100 100 CD 10_1101-2020_11_24_390039 389 66 % % NN 10_1101-2020_11_24_390039 389 67 efficiency efficiency NN 10_1101-2020_11_24_390039 389 68 ) ) -RRB- 10_1101-2020_11_24_390039 389 69 . . . 10_1101-2020_11_24_390039 390 1 Quantitations quantitation NNS 10_1101-2020_11_24_390039 390 2 are be VBP 10_1101-2020_11_24_390039 390 3 given give VBN 10_1101-2020_11_24_390039 390 4 as as IN 10_1101-2020_11_24_390039 390 5 mean±s.e.m mean±s.e.m NN 10_1101-2020_11_24_390039 390 6 for for IN 10_1101-2020_11_24_390039 390 7 independent independent JJ 10_1101-2020_11_24_390039 390 8 translation translation NN 10_1101-2020_11_24_390039 390 9 reactions reaction NNS 10_1101-2020_11_24_390039 390 10 performed perform VBN 10_1101-2020_11_24_390039 390 11 in in IN 10_1101-2020_11_24_390039 390 12 triplicate triplicate NN 10_1101-2020_11_24_390039 390 13 ( ( -LRB- 10_1101-2020_11_24_390039 390 14 n=3 n=3 NNP 10_1101-2020_11_24_390039 390 15 ) ) -RRB- 10_1101-2020_11_24_390039 390 16 and and CC 10_1101-2020_11_24_390039 390 17 statistical statistical JJ 10_1101-2020_11_24_390039 390 18 significance significance NN 10_1101-2020_11_24_390039 390 19 ( ( -LRB- 10_1101-2020_11_24_390039 390 20 one one CD 10_1101-2020_11_24_390039 390 21 - - HYPH 10_1101-2020_11_24_390039 390 22 way way NN 10_1101-2020_11_24_390039 390 23 ANOVA ANOVA NNP 10_1101-2020_11_24_390039 390 24 , , , 10_1101-2020_11_24_390039 390 25 DF DF NNP 10_1101-2020_11_24_390039 390 26 and and CC 10_1101-2020_11_24_390039 390 27 F F NNP 10_1101-2020_11_24_390039 390 28 values value NNS 10_1101-2020_11_24_390039 390 29 shown show VBN 10_1101-2020_11_24_390039 390 30 in in IN 10_1101-2020_11_24_390039 390 31 the the DT 10_1101-2020_11_24_390039 390 32 figure figure NN 10_1101-2020_11_24_390039 390 33 ) ) -RRB- 10_1101-2020_11_24_390039 390 34 was be VBD 10_1101-2020_11_24_390039 390 35 determined determine VBN 10_1101-2020_11_24_390039 390 36 using use VBG 10_1101-2020_11_24_390039 390 37 Dunnett Dunnett NNP 10_1101-2020_11_24_390039 390 38 ’s ’s POS 10_1101-2020_11_24_390039 390 39 multiple multiple JJ 10_1101-2020_11_24_390039 390 40 comparisons comparison NNS 10_1101-2020_11_24_390039 390 41 test test NN 10_1101-2020_11_24_390039 390 42 . . . 10_1101-2020_11_24_390039 391 1 Statistical statistical JJ 10_1101-2020_11_24_390039 391 2 significance significance NN 10_1101-2020_11_24_390039 391 3 : : : 10_1101-2020_11_24_390039 391 4 n.s n.s NNP 10_1101-2020_11_24_390039 391 5 . . NNP 10_1101-2020_11_24_390039 391 6 , , , 10_1101-2020_11_24_390039 391 7 non non JJ 10_1101-2020_11_24_390039 391 8 - - JJ 10_1101-2020_11_24_390039 391 9 significant significant JJ 10_1101-2020_11_24_390039 391 10 > > NN 10_1101-2020_11_24_390039 391 11 0.1 0.1 CD 10_1101-2020_11_24_390039 391 12 ; ; : 10_1101-2020_11_24_390039 391 13 * * NFP 10_1101-2020_11_24_390039 391 14 * * NFP 10_1101-2020_11_24_390039 391 15 * * NFP 10_1101-2020_11_24_390039 391 16 * * NFP 10_1101-2020_11_24_390039 391 17 , , , 10_1101-2020_11_24_390039 391 18 P p NN 10_1101-2020_11_24_390039 391 19 < < XX 10_1101-2020_11_24_390039 391 20 0.0001 0.0001 CD 10_1101-2020_11_24_390039 391 21 . . . 10_1101-2020_11_24_390039 392 1 Fig fig NN 10_1101-2020_11_24_390039 392 2 . . . 10_1101-2020_11_24_390039 393 1 3 3 LS 10_1101-2020_11_24_390039 393 2 . . . 10_1101-2020_11_24_390039 394 1 SARS SARS NNP 10_1101-2020_11_24_390039 394 2 - - HYPH 10_1101-2020_11_24_390039 394 3 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 394 4 proteins protein NNS 10_1101-2020_11_24_390039 394 5 are be VBP 10_1101-2020_11_24_390039 394 6 variably variably RB 10_1101-2020_11_24_390039 394 7 dependent dependent JJ 10_1101-2020_11_24_390039 394 8 on on IN 10_1101-2020_11_24_390039 394 9 the the DT 10_1101-2020_11_24_390039 394 10 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 394 11 complex complex NN 10_1101-2020_11_24_390039 394 12 and/or and/or CC 10_1101-2020_11_24_390039 394 13 the the DT 10_1101-2020_11_24_390039 394 14 EMC emc NN 10_1101-2020_11_24_390039 394 15 for for IN 10_1101-2020_11_24_390039 394 16 ER ER NNP 10_1101-2020_11_24_390039 394 17 membrane membrane NN 10_1101-2020_11_24_390039 394 18 translocation translocation NN 10_1101-2020_11_24_390039 394 19 / / SYM 10_1101-2020_11_24_390039 394 20 insertion insertion NN 10_1101-2020_11_24_390039 394 21 . . . 10_1101-2020_11_24_390039 395 1 ( ( -LRB- 10_1101-2020_11_24_390039 395 2 A a DT 10_1101-2020_11_24_390039 395 3 ) ) -RRB- 10_1101-2020_11_24_390039 395 4 Schematic schematic JJ 10_1101-2020_11_24_390039 395 5 of of IN 10_1101-2020_11_24_390039 395 6 in in FW 10_1101-2020_11_24_390039 395 7 vitro vitro FW 10_1101-2020_11_24_390039 395 8 ER ER NNP 10_1101-2020_11_24_390039 395 9 import import NN 10_1101-2020_11_24_390039 395 10 assay assay NN 10_1101-2020_11_24_390039 395 11 using use VBG 10_1101-2020_11_24_390039 395 12 control control NN 10_1101-2020_11_24_390039 395 13 SP SP NNP 10_1101-2020_11_24_390039 395 14 cells cell NNS 10_1101-2020_11_24_390039 395 15 , , , 10_1101-2020_11_24_390039 395 16 or or CC 10_1101-2020_11_24_390039 395 17 those those DT 10_1101-2020_11_24_390039 395 18 depleted deplete VBN 10_1101-2020_11_24_390039 395 19 of of IN 10_1101-2020_11_24_390039 395 20 a a DT 10_1101-2020_11_24_390039 395 21 subunit subunit NN 10_1101-2020_11_24_390039 395 22 of of IN 10_1101-2020_11_24_390039 395 23 the the DT 10_1101-2020_11_24_390039 395 24 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 395 25 complex complex NN 10_1101-2020_11_24_390039 395 26 and/or and/or CC 10_1101-2020_11_24_390039 395 27 the the DT 10_1101-2020_11_24_390039 395 28 EMC EMC NNP 10_1101-2020_11_24_390039 395 29 via via IN 10_1101-2020_11_24_390039 395 30 siRNA siRNA NNP 10_1101-2020_11_24_390039 395 31 . . . 10_1101-2020_11_24_390039 396 1 Following follow VBG 10_1101-2020_11_24_390039 396 2 translation translation NN 10_1101-2020_11_24_390039 396 3 , , , 10_1101-2020_11_24_390039 396 4 OPG2- OPG2- VBD 10_1101-2020_11_24_390039 396 5 tagged tag VBN 10_1101-2020_11_24_390039 396 6 translation translation NN 10_1101-2020_11_24_390039 396 7 products product NNS 10_1101-2020_11_24_390039 396 8 ( ( -LRB- 10_1101-2020_11_24_390039 396 9 i.e. i.e. FW 10_1101-2020_11_24_390039 397 1 membrane membrane NN 10_1101-2020_11_24_390039 397 2 - - HYPH 10_1101-2020_11_24_390039 397 3 associated associate VBN 10_1101-2020_11_24_390039 397 4 and and CC 10_1101-2020_11_24_390039 397 5 non non JJ 10_1101-2020_11_24_390039 397 6 - - JJ 10_1101-2020_11_24_390039 397 7 targeted target VBN 10_1101-2020_11_24_390039 397 8 nascent nascent JJ 10_1101-2020_11_24_390039 397 9 chains chain NNS 10_1101-2020_11_24_390039 397 10 ) ) -RRB- 10_1101-2020_11_24_390039 397 11 were be VBD 10_1101-2020_11_24_390039 397 12 immunoprecipitated immunoprecipitate VBN 10_1101-2020_11_24_390039 397 13 , , , 10_1101-2020_11_24_390039 397 14 resolved resolve VBN 10_1101-2020_11_24_390039 397 15 by by IN 10_1101-2020_11_24_390039 397 16 SDS SDS NNP 10_1101-2020_11_24_390039 397 17 - - HYPH 10_1101-2020_11_24_390039 397 18 PAGE PAGE NNP 10_1101-2020_11_24_390039 397 19 and and CC 10_1101-2020_11_24_390039 397 20 analysed analyse VBN 10_1101-2020_11_24_390039 397 21 by by IN 10_1101-2020_11_24_390039 397 22 phosphorimaging phosphorimage VBG 10_1101-2020_11_24_390039 397 23 . . . 10_1101-2020_11_24_390039 398 1 OPG2-tagged opg2-tagged JJ 10_1101-2020_11_24_390039 398 2 variants variant NNS 10_1101-2020_11_24_390039 398 3 of of IN 10_1101-2020_11_24_390039 398 4 the the DT 10_1101-2020_11_24_390039 398 5 SARS SARS NNP 10_1101-2020_11_24_390039 398 6 - - HYPH 10_1101-2020_11_24_390039 398 7 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 398 8 ( ( -LRB- 10_1101-2020_11_24_390039 398 9 B b NN 10_1101-2020_11_24_390039 398 10 ) ) -RRB- 10_1101-2020_11_24_390039 398 11 spike spike NNP 10_1101-2020_11_24_390039 398 12 ( ( -LRB- 10_1101-2020_11_24_390039 398 13 S- s- XX 10_1101-2020_11_24_390039 398 14 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 398 15 ) ) -RRB- 10_1101-2020_11_24_390039 398 16 , , , 10_1101-2020_11_24_390039 398 17 ( ( -LRB- 10_1101-2020_11_24_390039 398 18 C c NN 10_1101-2020_11_24_390039 398 19 ) ) -RRB- 10_1101-2020_11_24_390039 398 20 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 398 21 ( ( -LRB- 10_1101-2020_11_24_390039 398 22 ORF8-OPG2 ORF8-OPG2 NNP 10_1101-2020_11_24_390039 398 23 ) ) -RRB- 10_1101-2020_11_24_390039 398 24 , , , 10_1101-2020_11_24_390039 398 25 ( ( -LRB- 10_1101-2020_11_24_390039 398 26 D d NN 10_1101-2020_11_24_390039 398 27 ) ) -RRB- 10_1101-2020_11_24_390039 398 28 envelope envelope NNP 10_1101-2020_11_24_390039 398 29 ( ( -LRB- 10_1101-2020_11_24_390039 398 30 OPG2-E OPG2-E NNP 10_1101-2020_11_24_390039 398 31 ) ) -RRB- 10_1101-2020_11_24_390039 398 32 and and CC 10_1101-2020_11_24_390039 398 33 ( ( -LRB- 10_1101-2020_11_24_390039 398 34 E e NN 10_1101-2020_11_24_390039 398 35 ) ) -RRB- 10_1101-2020_11_24_390039 398 36 ORF6 orf6 ADD 10_1101-2020_11_24_390039 398 37 ( ( -LRB- 10_1101-2020_11_24_390039 398 38 OPG2- OPG2- NNP 10_1101-2020_11_24_390039 398 39 ORF6-OPG2 ORF6-OPG2 NNP 10_1101-2020_11_24_390039 398 40 species specie NNS 10_1101-2020_11_24_390039 398 41 ( ( -LRB- 10_1101-2020_11_24_390039 398 42 labelled label VBN 10_1101-2020_11_24_390039 398 43 as as IN 10_1101-2020_11_24_390039 398 44 for for IN 10_1101-2020_11_24_390039 398 45 Fig Fig NNP 10_1101-2020_11_24_390039 398 46 . . . 10_1101-2020_11_24_390039 399 1 2 2 LS 10_1101-2020_11_24_390039 399 2 ) ) -RRB- 10_1101-2020_11_24_390039 399 3 were be VBD 10_1101-2020_11_24_390039 399 4 synthesised synthesise VBN 10_1101-2020_11_24_390039 399 5 in in IN 10_1101-2020_11_24_390039 399 6 rabbit rabbit NN 10_1101-2020_11_24_390039 399 7 reticulocyte reticulocyte NNP 10_1101-2020_11_24_390039 399 8 lysate lysate NN 10_1101-2020_11_24_390039 399 9 supplemented supplement VBN 10_1101-2020_11_24_390039 399 10 with with IN 10_1101-2020_11_24_390039 399 11 control control NN 10_1101-2020_11_24_390039 399 12 SP SP NNP 10_1101-2020_11_24_390039 399 13 cells cell NNS 10_1101-2020_11_24_390039 399 14 ( ( -LRB- 10_1101-2020_11_24_390039 399 15 lanes lanes NNP 10_1101-2020_11_24_390039 399 16 1 1 CD 10_1101-2020_11_24_390039 399 17 - - SYM 10_1101-2020_11_24_390039 399 18 2 2 CD 10_1101-2020_11_24_390039 399 19 ) ) -RRB- 10_1101-2020_11_24_390039 399 20 or or CC 10_1101-2020_11_24_390039 399 21 those those DT 10_1101-2020_11_24_390039 399 22 with with IN 10_1101-2020_11_24_390039 399 23 impaired impaired JJ 10_1101-2020_11_24_390039 399 24 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 399 25 and/or and/or CC 10_1101-2020_11_24_390039 399 26 EMC EMC NNP 10_1101-2020_11_24_390039 399 27 function function NN 10_1101-2020_11_24_390039 399 28 ( ( -LRB- 10_1101-2020_11_24_390039 399 29 lanes lanes NNP 10_1101-2020_11_24_390039 399 30 3 3 CD 10_1101-2020_11_24_390039 399 31 - - SYM 10_1101-2020_11_24_390039 399 32 6 6 CD 10_1101-2020_11_24_390039 399 33 ) ) -RRB- 10_1101-2020_11_24_390039 399 34 . . . 10_1101-2020_11_24_390039 400 1 Radiolabelled radiolabelle VBN 10_1101-2020_11_24_390039 400 2 products product NNS 10_1101-2020_11_24_390039 400 3 were be VBD 10_1101-2020_11_24_390039 400 4 recovered recover VBN 10_1101-2020_11_24_390039 400 5 and and CC 10_1101-2020_11_24_390039 400 6 analysed analyse VBN 10_1101-2020_11_24_390039 400 7 as as IN 10_1101-2020_11_24_390039 400 8 in in IN 10_1101-2020_11_24_390039 400 9 ( ( -LRB- 10_1101-2020_11_24_390039 400 10 A a NN 10_1101-2020_11_24_390039 400 11 ) ) -RRB- 10_1101-2020_11_24_390039 400 12 . . . 10_1101-2020_11_24_390039 401 1 Membrane membrane NN 10_1101-2020_11_24_390039 401 2 translocation translocation NN 10_1101-2020_11_24_390039 401 3 / / SYM 10_1101-2020_11_24_390039 401 4 insertion insertion NN 10_1101-2020_11_24_390039 401 5 efficiency efficiency NN 10_1101-2020_11_24_390039 401 6 was be VBD 10_1101-2020_11_24_390039 401 7 determined determine VBN 10_1101-2020_11_24_390039 401 8 using use VBG 10_1101-2020_11_24_390039 401 9 the the DT 10_1101-2020_11_24_390039 401 10 ratio ratio NN 10_1101-2020_11_24_390039 401 11 of of IN 10_1101-2020_11_24_390039 401 12 the the DT 10_1101-2020_11_24_390039 401 13 N n NN 10_1101-2020_11_24_390039 401 14 - - HYPH 10_1101-2020_11_24_390039 401 15 glycosylation glycosylation NN 10_1101-2020_11_24_390039 401 16 of of IN 10_1101-2020_11_24_390039 401 17 lumenal lumenal JJ 10_1101-2020_11_24_390039 401 18 domains domain NNS 10_1101-2020_11_24_390039 401 19 , , , 10_1101-2020_11_24_390039 401 20 identified identify VBN 10_1101-2020_11_24_390039 401 21 using use VBG 10_1101-2020_11_24_390039 401 22 Endo Endo NNP 10_1101-2020_11_24_390039 401 23 H H NNP 10_1101-2020_11_24_390039 401 24 ( ( -LRB- 10_1101-2020_11_24_390039 401 25 EH EH NNP 10_1101-2020_11_24_390039 401 26 , , , 10_1101-2020_11_24_390039 401 27 lane lane NNP 10_1101-2020_11_24_390039 401 28 1 1 CD 10_1101-2020_11_24_390039 401 29 ) ) -RRB- 10_1101-2020_11_24_390039 401 30 , , , 10_1101-2020_11_24_390039 401 31 relative relative JJ 10_1101-2020_11_24_390039 401 32 to to IN 10_1101-2020_11_24_390039 401 33 the the DT 10_1101-2020_11_24_390039 401 34 NT NT NNP 10_1101-2020_11_24_390039 401 35 control control NN 10_1101-2020_11_24_390039 401 36 ( ( -LRB- 10_1101-2020_11_24_390039 401 37 set set VBN 10_1101-2020_11_24_390039 401 38 to to IN 10_1101-2020_11_24_390039 401 39 100 100 CD 10_1101-2020_11_24_390039 401 40 % % NN 10_1101-2020_11_24_390039 401 41 translocation translocation NN 10_1101-2020_11_24_390039 401 42 / / SYM 10_1101-2020_11_24_390039 401 43 insertion insertion NN 10_1101-2020_11_24_390039 401 44 efficiency efficiency NN 10_1101-2020_11_24_390039 401 45 ) ) -RRB- 10_1101-2020_11_24_390039 401 46 . . . 10_1101-2020_11_24_390039 402 1 Quantitations quantitation NNS 10_1101-2020_11_24_390039 402 2 ( ( -LRB- 10_1101-2020_11_24_390039 402 3 n=3 n=3 NNP 10_1101-2020_11_24_390039 402 4 ) ) -RRB- 10_1101-2020_11_24_390039 402 5 and and CC 10_1101-2020_11_24_390039 402 6 statistical statistical JJ 10_1101-2020_11_24_390039 402 7 significance significance NN 10_1101-2020_11_24_390039 402 8 ( ( -LRB- 10_1101-2020_11_24_390039 402 9 two two CD 10_1101-2020_11_24_390039 402 10 - - HYPH 10_1101-2020_11_24_390039 402 11 way way NN 10_1101-2020_11_24_390039 402 12 ANOVA ANOVA NNP 10_1101-2020_11_24_390039 402 13 , , , 10_1101-2020_11_24_390039 402 14 DF DF NNP 10_1101-2020_11_24_390039 402 15 and and CC 10_1101-2020_11_24_390039 402 16 F F NNP 10_1101-2020_11_24_390039 402 17 values value NNS 10_1101-2020_11_24_390039 402 18 shown show VBN 10_1101-2020_11_24_390039 402 19 in in IN 10_1101-2020_11_24_390039 402 20 the the DT 10_1101-2020_11_24_390039 402 21 figure figure NN 10_1101-2020_11_24_390039 402 22 ) ) -RRB- 10_1101-2020_11_24_390039 402 23 determined determine VBD 10_1101-2020_11_24_390039 402 24 as as IN 10_1101-2020_11_24_390039 402 25 for for IN 10_1101-2020_11_24_390039 402 26 Figure figure NN 10_1101-2020_11_24_390039 402 27 2 2 CD 10_1101-2020_11_24_390039 402 28 . . . 10_1101-2020_11_24_390039 403 1 Statistical statistical JJ 10_1101-2020_11_24_390039 403 2 significance significance NN 10_1101-2020_11_24_390039 403 3 : : : 10_1101-2020_11_24_390039 403 4 n.s n.s NNP 10_1101-2020_11_24_390039 403 5 . . NNP 10_1101-2020_11_24_390039 403 6 , , , 10_1101-2020_11_24_390039 403 7 non non JJ 10_1101-2020_11_24_390039 403 8 - - JJ 10_1101-2020_11_24_390039 403 9 significant significant JJ 10_1101-2020_11_24_390039 403 10 > > NN 10_1101-2020_11_24_390039 403 11 0.1 0.1 CD 10_1101-2020_11_24_390039 403 12 ; ; : 10_1101-2020_11_24_390039 403 13 * * NFP 10_1101-2020_11_24_390039 403 14 , , , 10_1101-2020_11_24_390039 403 15 P p NN 10_1101-2020_11_24_390039 403 16 < < XX 10_1101-2020_11_24_390039 403 17 0.05 0.05 XX 10_1101-2020_11_24_390039 403 18 ; ; : 10_1101-2020_11_24_390039 403 19 * * NFP 10_1101-2020_11_24_390039 403 20 * * NFP 10_1101-2020_11_24_390039 403 21 , , , 10_1101-2020_11_24_390039 403 22 P p NN 10_1101-2020_11_24_390039 403 23 < < XX 10_1101-2020_11_24_390039 403 24 0.01 0.01 XX 10_1101-2020_11_24_390039 403 25 ; ; : 10_1101-2020_11_24_390039 403 26 * * NFP 10_1101-2020_11_24_390039 403 27 * * NFP 10_1101-2020_11_24_390039 403 28 * * NFP 10_1101-2020_11_24_390039 403 29 , , , 10_1101-2020_11_24_390039 403 30 P p NN 10_1101-2020_11_24_390039 403 31 < < XX 10_1101-2020_11_24_390039 403 32 0.001 0.001 CD 10_1101-2020_11_24_390039 403 33 ; ; : 10_1101-2020_11_24_390039 403 34 * * NFP 10_1101-2020_11_24_390039 403 35 * * NFP 10_1101-2020_11_24_390039 403 36 * * NFP 10_1101-2020_11_24_390039 403 37 * * NFP 10_1101-2020_11_24_390039 403 38 , , , 10_1101-2020_11_24_390039 403 39 P p NN 10_1101-2020_11_24_390039 403 40 < < XX 10_1101-2020_11_24_390039 403 41 0.0001 0.0001 CD 10_1101-2020_11_24_390039 403 42 . . . 10_1101-2020_11_24_390039 404 1 .CC .CC NFP 10_1101-2020_11_24_390039 404 2 - - : 10_1101-2020_11_24_390039 404 3 BY by IN 10_1101-2020_11_24_390039 404 4 - - HYPH 10_1101-2020_11_24_390039 404 5 ND ND NNP 10_1101-2020_11_24_390039 404 6 4.0 4.0 CD 10_1101-2020_11_24_390039 404 7 International international JJ 10_1101-2020_11_24_390039 404 8 licensemade licensemade NN 10_1101-2020_11_24_390039 404 9 available available JJ 10_1101-2020_11_24_390039 404 10 under under IN 10_1101-2020_11_24_390039 404 11 a a DT 10_1101-2020_11_24_390039 404 12 ( ( -LRB- 10_1101-2020_11_24_390039 404 13 which which WDT 10_1101-2020_11_24_390039 404 14 was be VBD 10_1101-2020_11_24_390039 404 15 not not RB 10_1101-2020_11_24_390039 404 16 certified certify VBN 10_1101-2020_11_24_390039 404 17 by by IN 10_1101-2020_11_24_390039 404 18 peer peer NN 10_1101-2020_11_24_390039 404 19 review review NN 10_1101-2020_11_24_390039 404 20 ) ) -RRB- 10_1101-2020_11_24_390039 404 21 is be VBZ 10_1101-2020_11_24_390039 404 22 the the DT 10_1101-2020_11_24_390039 404 23 author author NN 10_1101-2020_11_24_390039 404 24 / / SYM 10_1101-2020_11_24_390039 404 25 funder funder NN 10_1101-2020_11_24_390039 404 26 , , , 10_1101-2020_11_24_390039 404 27 who who WP 10_1101-2020_11_24_390039 404 28 has have VBZ 10_1101-2020_11_24_390039 404 29 granted grant VBN 10_1101-2020_11_24_390039 404 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 404 31 a a DT 10_1101-2020_11_24_390039 404 32 license license NN 10_1101-2020_11_24_390039 404 33 to to TO 10_1101-2020_11_24_390039 404 34 display display VB 10_1101-2020_11_24_390039 404 35 the the DT 10_1101-2020_11_24_390039 404 36 preprint preprint NN 10_1101-2020_11_24_390039 404 37 in in IN 10_1101-2020_11_24_390039 404 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 404 39 . . . 10_1101-2020_11_24_390039 405 1 It -PRON- PRP 10_1101-2020_11_24_390039 405 2 is be VBZ 10_1101-2020_11_24_390039 405 3 The the DT 10_1101-2020_11_24_390039 405 4 copyright copyright NN 10_1101-2020_11_24_390039 405 5 holder holder NN 10_1101-2020_11_24_390039 405 6 for for IN 10_1101-2020_11_24_390039 405 7 this this DT 10_1101-2020_11_24_390039 405 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 405 9 version version NN 10_1101-2020_11_24_390039 405 10 posted post VBD 10_1101-2020_11_24_390039 405 11 January January NNP 10_1101-2020_11_24_390039 405 12 5 5 CD 10_1101-2020_11_24_390039 405 13 , , , 10_1101-2020_11_24_390039 405 14 2021 2021 CD 10_1101-2020_11_24_390039 405 15 . . . 10_1101-2020_11_24_390039 405 16 ; ; : 10_1101-2020_11_24_390039 405 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 405 18 : : : 10_1101-2020_11_24_390039 405 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 405 20 preprint preprint JJ 10_1101-2020_11_24_390039 405 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NNP 10_1101-2020_11_24_390039 405 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NN 10_1101-2020_11_24_390039 405 23 .CC .CC NFP 10_1101-2020_11_24_390039 405 24 - - : 10_1101-2020_11_24_390039 405 25 BY by IN 10_1101-2020_11_24_390039 405 26 - - HYPH 10_1101-2020_11_24_390039 405 27 ND ND NNP 10_1101-2020_11_24_390039 405 28 4.0 4.0 CD 10_1101-2020_11_24_390039 405 29 International international JJ 10_1101-2020_11_24_390039 405 30 licensemade licensemade NN 10_1101-2020_11_24_390039 405 31 available available JJ 10_1101-2020_11_24_390039 405 32 under under IN 10_1101-2020_11_24_390039 405 33 a a DT 10_1101-2020_11_24_390039 405 34 ( ( -LRB- 10_1101-2020_11_24_390039 405 35 which which WDT 10_1101-2020_11_24_390039 405 36 was be VBD 10_1101-2020_11_24_390039 405 37 not not RB 10_1101-2020_11_24_390039 405 38 certified certify VBN 10_1101-2020_11_24_390039 405 39 by by IN 10_1101-2020_11_24_390039 405 40 peer peer NN 10_1101-2020_11_24_390039 405 41 review review NN 10_1101-2020_11_24_390039 405 42 ) ) -RRB- 10_1101-2020_11_24_390039 405 43 is be VBZ 10_1101-2020_11_24_390039 405 44 the the DT 10_1101-2020_11_24_390039 405 45 author author NN 10_1101-2020_11_24_390039 405 46 / / SYM 10_1101-2020_11_24_390039 405 47 funder funder NN 10_1101-2020_11_24_390039 405 48 , , , 10_1101-2020_11_24_390039 405 49 who who WP 10_1101-2020_11_24_390039 405 50 has have VBZ 10_1101-2020_11_24_390039 405 51 granted grant VBN 10_1101-2020_11_24_390039 405 52 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 405 53 a a DT 10_1101-2020_11_24_390039 405 54 license license NN 10_1101-2020_11_24_390039 405 55 to to TO 10_1101-2020_11_24_390039 405 56 display display VB 10_1101-2020_11_24_390039 405 57 the the DT 10_1101-2020_11_24_390039 405 58 preprint preprint NN 10_1101-2020_11_24_390039 405 59 in in IN 10_1101-2020_11_24_390039 405 60 perpetuity perpetuity NN 10_1101-2020_11_24_390039 405 61 . . . 10_1101-2020_11_24_390039 406 1 It -PRON- PRP 10_1101-2020_11_24_390039 406 2 is be VBZ 10_1101-2020_11_24_390039 406 3 The the DT 10_1101-2020_11_24_390039 406 4 copyright copyright NN 10_1101-2020_11_24_390039 406 5 holder holder NN 10_1101-2020_11_24_390039 406 6 for for IN 10_1101-2020_11_24_390039 406 7 this this DT 10_1101-2020_11_24_390039 406 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 406 9 version version NN 10_1101-2020_11_24_390039 406 10 posted post VBD 10_1101-2020_11_24_390039 406 11 January January NNP 10_1101-2020_11_24_390039 406 12 5 5 CD 10_1101-2020_11_24_390039 406 13 , , , 10_1101-2020_11_24_390039 406 14 2021 2021 CD 10_1101-2020_11_24_390039 406 15 . . . 10_1101-2020_11_24_390039 406 16 ; ; : 10_1101-2020_11_24_390039 406 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 406 18 : : : 10_1101-2020_11_24_390039 406 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 406 20 preprint preprint NN 10_1101-2020_11_24_390039 406 21 mqbssysh mqbssysh NNS 10_1101-2020_11_24_390039 406 22 Typewritten Typewritten NNP 10_1101-2020_11_24_390039 406 23 Text Text NNP 10_1101-2020_11_24_390039 406 24 Figure Figure NNP 10_1101-2020_11_24_390039 406 25 1 1 CD 10_1101-2020_11_24_390039 406 26 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 406 27 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NN 10_1101-2020_11_24_390039 406 28 .CC .CC NFP 10_1101-2020_11_24_390039 406 29 - - : 10_1101-2020_11_24_390039 406 30 BY by IN 10_1101-2020_11_24_390039 406 31 - - HYPH 10_1101-2020_11_24_390039 406 32 ND ND NNP 10_1101-2020_11_24_390039 406 33 4.0 4.0 CD 10_1101-2020_11_24_390039 406 34 International international JJ 10_1101-2020_11_24_390039 406 35 licensemade licensemade NN 10_1101-2020_11_24_390039 406 36 available available JJ 10_1101-2020_11_24_390039 406 37 under under IN 10_1101-2020_11_24_390039 406 38 a a DT 10_1101-2020_11_24_390039 406 39 ( ( -LRB- 10_1101-2020_11_24_390039 406 40 which which WDT 10_1101-2020_11_24_390039 406 41 was be VBD 10_1101-2020_11_24_390039 406 42 not not RB 10_1101-2020_11_24_390039 406 43 certified certify VBN 10_1101-2020_11_24_390039 406 44 by by IN 10_1101-2020_11_24_390039 406 45 peer peer NN 10_1101-2020_11_24_390039 406 46 review review NN 10_1101-2020_11_24_390039 406 47 ) ) -RRB- 10_1101-2020_11_24_390039 406 48 is be VBZ 10_1101-2020_11_24_390039 406 49 the the DT 10_1101-2020_11_24_390039 406 50 author author NN 10_1101-2020_11_24_390039 406 51 / / SYM 10_1101-2020_11_24_390039 406 52 funder funder NN 10_1101-2020_11_24_390039 406 53 , , , 10_1101-2020_11_24_390039 406 54 who who WP 10_1101-2020_11_24_390039 406 55 has have VBZ 10_1101-2020_11_24_390039 406 56 granted grant VBN 10_1101-2020_11_24_390039 406 57 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 406 58 a a DT 10_1101-2020_11_24_390039 406 59 license license NN 10_1101-2020_11_24_390039 406 60 to to TO 10_1101-2020_11_24_390039 406 61 display display VB 10_1101-2020_11_24_390039 406 62 the the DT 10_1101-2020_11_24_390039 406 63 preprint preprint NN 10_1101-2020_11_24_390039 406 64 in in IN 10_1101-2020_11_24_390039 406 65 perpetuity perpetuity NN 10_1101-2020_11_24_390039 406 66 . . . 10_1101-2020_11_24_390039 407 1 It -PRON- PRP 10_1101-2020_11_24_390039 407 2 is be VBZ 10_1101-2020_11_24_390039 407 3 The the DT 10_1101-2020_11_24_390039 407 4 copyright copyright NN 10_1101-2020_11_24_390039 407 5 holder holder NN 10_1101-2020_11_24_390039 407 6 for for IN 10_1101-2020_11_24_390039 407 7 this this DT 10_1101-2020_11_24_390039 407 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 407 9 version version NN 10_1101-2020_11_24_390039 407 10 posted post VBD 10_1101-2020_11_24_390039 407 11 January January NNP 10_1101-2020_11_24_390039 407 12 5 5 CD 10_1101-2020_11_24_390039 407 13 , , , 10_1101-2020_11_24_390039 407 14 2021 2021 CD 10_1101-2020_11_24_390039 407 15 . . . 10_1101-2020_11_24_390039 407 16 ; ; : 10_1101-2020_11_24_390039 407 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 407 18 : : : 10_1101-2020_11_24_390039 407 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 407 20 preprint preprint NN 10_1101-2020_11_24_390039 407 21 mqbssysh mqbssysh NNS 10_1101-2020_11_24_390039 407 22 Typewritten Typewritten NNP 10_1101-2020_11_24_390039 407 23 Text Text NNP 10_1101-2020_11_24_390039 407 24 Figure Figure NNP 10_1101-2020_11_24_390039 407 25 2 2 CD 10_1101-2020_11_24_390039 407 26 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 407 27 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NN 10_1101-2020_11_24_390039 407 28 .CC .CC NFP 10_1101-2020_11_24_390039 407 29 - - : 10_1101-2020_11_24_390039 407 30 BY by IN 10_1101-2020_11_24_390039 407 31 - - HYPH 10_1101-2020_11_24_390039 407 32 ND ND NNP 10_1101-2020_11_24_390039 407 33 4.0 4.0 CD 10_1101-2020_11_24_390039 407 34 International international JJ 10_1101-2020_11_24_390039 407 35 licensemade licensemade NN 10_1101-2020_11_24_390039 407 36 available available JJ 10_1101-2020_11_24_390039 407 37 under under IN 10_1101-2020_11_24_390039 407 38 a a DT 10_1101-2020_11_24_390039 407 39 ( ( -LRB- 10_1101-2020_11_24_390039 407 40 which which WDT 10_1101-2020_11_24_390039 407 41 was be VBD 10_1101-2020_11_24_390039 407 42 not not RB 10_1101-2020_11_24_390039 407 43 certified certify VBN 10_1101-2020_11_24_390039 407 44 by by IN 10_1101-2020_11_24_390039 407 45 peer peer NN 10_1101-2020_11_24_390039 407 46 review review NN 10_1101-2020_11_24_390039 407 47 ) ) -RRB- 10_1101-2020_11_24_390039 407 48 is be VBZ 10_1101-2020_11_24_390039 407 49 the the DT 10_1101-2020_11_24_390039 407 50 author author NN 10_1101-2020_11_24_390039 407 51 / / SYM 10_1101-2020_11_24_390039 407 52 funder funder NN 10_1101-2020_11_24_390039 407 53 , , , 10_1101-2020_11_24_390039 407 54 who who WP 10_1101-2020_11_24_390039 407 55 has have VBZ 10_1101-2020_11_24_390039 407 56 granted grant VBN 10_1101-2020_11_24_390039 407 57 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 407 58 a a DT 10_1101-2020_11_24_390039 407 59 license license NN 10_1101-2020_11_24_390039 407 60 to to TO 10_1101-2020_11_24_390039 407 61 display display VB 10_1101-2020_11_24_390039 407 62 the the DT 10_1101-2020_11_24_390039 407 63 preprint preprint NN 10_1101-2020_11_24_390039 407 64 in in IN 10_1101-2020_11_24_390039 407 65 perpetuity perpetuity NN 10_1101-2020_11_24_390039 407 66 . . . 10_1101-2020_11_24_390039 408 1 It -PRON- PRP 10_1101-2020_11_24_390039 408 2 is be VBZ 10_1101-2020_11_24_390039 408 3 The the DT 10_1101-2020_11_24_390039 408 4 copyright copyright NN 10_1101-2020_11_24_390039 408 5 holder holder NN 10_1101-2020_11_24_390039 408 6 for for IN 10_1101-2020_11_24_390039 408 7 this this DT 10_1101-2020_11_24_390039 408 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 408 9 version version NN 10_1101-2020_11_24_390039 408 10 posted post VBD 10_1101-2020_11_24_390039 408 11 January January NNP 10_1101-2020_11_24_390039 408 12 5 5 CD 10_1101-2020_11_24_390039 408 13 , , , 10_1101-2020_11_24_390039 408 14 2021 2021 CD 10_1101-2020_11_24_390039 408 15 . . . 10_1101-2020_11_24_390039 408 16 ; ; : 10_1101-2020_11_24_390039 408 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 408 18 : : : 10_1101-2020_11_24_390039 408 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 408 20 preprint preprint NN 10_1101-2020_11_24_390039 408 21 mqbssysh mqbssysh NNS 10_1101-2020_11_24_390039 408 22 Typewritten Typewritten NNP 10_1101-2020_11_24_390039 408 23 Text Text NNP 10_1101-2020_11_24_390039 408 24 Figure Figure NNP 10_1101-2020_11_24_390039 408 25 3 3 CD 10_1101-2020_11_24_390039 408 26 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 408 27 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NN 10_1101-2020_11_24_390039 408 28 Supplementary supplementary JJ 10_1101-2020_11_24_390039 408 29 information information NN 10_1101-2020_11_24_390039 408 30 for for IN 10_1101-2020_11_24_390039 408 31 Ipomoeassin Ipomoeassin NNP 10_1101-2020_11_24_390039 408 32 - - HYPH 10_1101-2020_11_24_390039 408 33 F F NNP 10_1101-2020_11_24_390039 408 34 inhibits inhibit VBZ 10_1101-2020_11_24_390039 408 35 the the DT 10_1101-2020_11_24_390039 408 36 in in FW 10_1101-2020_11_24_390039 408 37 vitro vitro FW 10_1101-2020_11_24_390039 408 38 biogenesis biogenesis NN 10_1101-2020_11_24_390039 408 39 of of IN 10_1101-2020_11_24_390039 408 40 the the DT 10_1101-2020_11_24_390039 408 41 SARS SARS NNP 10_1101-2020_11_24_390039 408 42 - - HYPH 10_1101-2020_11_24_390039 408 43 CoV-2 CoV-2 NNP 10_1101-2020_11_24_390039 408 44 spike spike JJ 10_1101-2020_11_24_390039 408 45 protein protein NN 10_1101-2020_11_24_390039 408 46 and and CC 10_1101-2020_11_24_390039 408 47 its -PRON- PRP$ 10_1101-2020_11_24_390039 408 48 host host NN 10_1101-2020_11_24_390039 408 49 cell cell NN 10_1101-2020_11_24_390039 408 50 membrane membrane NN 10_1101-2020_11_24_390039 408 51 receptor receptor NN 10_1101-2020_11_24_390039 408 52 Sa Sa NNP 10_1101-2020_11_24_390039 408 53 a a DT 10_1101-2020_11_24_390039 408 54 O o NN 10_1101-2020_11_24_390039 408 55 Kee Kee NNP 10_1101-2020_11_24_390039 408 56 e e NN 10_1101-2020_11_24_390039 408 57 , , , 10_1101-2020_11_24_390039 408 58 Pe Pe NNP 10_1101-2020_11_24_390039 408 59 e e VBZ 10_1101-2020_11_24_390039 408 60 a a DT 10_1101-2020_11_24_390039 408 61 Roboti Roboti NNP 10_1101-2020_11_24_390039 408 62 , , , 10_1101-2020_11_24_390039 408 63 Kwabena Kwabena NNP 10_1101-2020_11_24_390039 408 64 B. B. NNP 10_1101-2020_11_24_390039 408 65 Duah Duah NNP 10_1101-2020_11_24_390039 408 66 , , , 10_1101-2020_11_24_390039 408 67 Guanghui Guanghui NNP 10_1101-2020_11_24_390039 408 68 Zong Zong NNP 10_1101-2020_11_24_390039 408 69 , , , 10_1101-2020_11_24_390039 408 70 Hayden Hayden NNP 10_1101-2020_11_24_390039 408 71 Scheider Scheider NNP 10_1101-2020_11_24_390039 408 72 , , , 10_1101-2020_11_24_390039 408 73 Wei Wei NNP 10_1101-2020_11_24_390039 408 74 Q. Q. NNP 10_1101-2020_11_24_390039 408 75 Shi Shi NNP 10_1101-2020_11_24_390039 408 76 and and CC 10_1101-2020_11_24_390039 408 77 Stephen Stephen NNP 10_1101-2020_11_24_390039 408 78 High High NNP 10_1101-2020_11_24_390039 408 79 .CC .CC : 10_1101-2020_11_24_390039 408 80 - - HYPH 10_1101-2020_11_24_390039 408 81 BY by IN 10_1101-2020_11_24_390039 408 82 - - HYPH 10_1101-2020_11_24_390039 408 83 ND ND NNP 10_1101-2020_11_24_390039 408 84 4.0 4.0 CD 10_1101-2020_11_24_390039 408 85 International international JJ 10_1101-2020_11_24_390039 408 86 licensemade licensemade NN 10_1101-2020_11_24_390039 408 87 available available JJ 10_1101-2020_11_24_390039 408 88 under under IN 10_1101-2020_11_24_390039 408 89 a a DT 10_1101-2020_11_24_390039 408 90 ( ( -LRB- 10_1101-2020_11_24_390039 408 91 which which WDT 10_1101-2020_11_24_390039 408 92 was be VBD 10_1101-2020_11_24_390039 408 93 not not RB 10_1101-2020_11_24_390039 408 94 certified certify VBN 10_1101-2020_11_24_390039 408 95 by by IN 10_1101-2020_11_24_390039 408 96 peer peer NN 10_1101-2020_11_24_390039 408 97 review review NN 10_1101-2020_11_24_390039 408 98 ) ) -RRB- 10_1101-2020_11_24_390039 408 99 is be VBZ 10_1101-2020_11_24_390039 408 100 the the DT 10_1101-2020_11_24_390039 408 101 author author NN 10_1101-2020_11_24_390039 408 102 / / SYM 10_1101-2020_11_24_390039 408 103 funder funder NN 10_1101-2020_11_24_390039 408 104 , , , 10_1101-2020_11_24_390039 408 105 who who WP 10_1101-2020_11_24_390039 408 106 has have VBZ 10_1101-2020_11_24_390039 408 107 granted grant VBN 10_1101-2020_11_24_390039 408 108 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 408 109 a a DT 10_1101-2020_11_24_390039 408 110 license license NN 10_1101-2020_11_24_390039 408 111 to to TO 10_1101-2020_11_24_390039 408 112 display display VB 10_1101-2020_11_24_390039 408 113 the the DT 10_1101-2020_11_24_390039 408 114 preprint preprint NN 10_1101-2020_11_24_390039 408 115 in in IN 10_1101-2020_11_24_390039 408 116 perpetuity perpetuity NN 10_1101-2020_11_24_390039 408 117 . . . 10_1101-2020_11_24_390039 409 1 It -PRON- PRP 10_1101-2020_11_24_390039 409 2 is be VBZ 10_1101-2020_11_24_390039 409 3 The the DT 10_1101-2020_11_24_390039 409 4 copyright copyright NN 10_1101-2020_11_24_390039 409 5 holder holder NN 10_1101-2020_11_24_390039 409 6 for for IN 10_1101-2020_11_24_390039 409 7 this this DT 10_1101-2020_11_24_390039 409 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 409 9 version version NN 10_1101-2020_11_24_390039 409 10 posted post VBD 10_1101-2020_11_24_390039 409 11 January January NNP 10_1101-2020_11_24_390039 409 12 5 5 CD 10_1101-2020_11_24_390039 409 13 , , , 10_1101-2020_11_24_390039 409 14 2021 2021 CD 10_1101-2020_11_24_390039 409 15 . . . 10_1101-2020_11_24_390039 409 16 ; ; : 10_1101-2020_11_24_390039 409 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 409 18 : : : 10_1101-2020_11_24_390039 409 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 409 20 preprint preprint JJ 10_1101-2020_11_24_390039 409 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 409 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NN 10_1101-2020_11_24_390039 409 23 Page page NN 10_1101-2020_11_24_390039 409 24 2 2 CD 10_1101-2020_11_24_390039 409 25 of of IN 10_1101-2020_11_24_390039 409 26 5 5 CD 10_1101-2020_11_24_390039 409 27 Fig Fig NNP 10_1101-2020_11_24_390039 409 28 . . . 10_1101-2020_11_24_390039 410 1 S1 S1 NNS 10_1101-2020_11_24_390039 410 2 . . . 10_1101-2020_11_24_390039 411 1 Additional additional JJ 10_1101-2020_11_24_390039 411 2 studies study NNS 10_1101-2020_11_24_390039 411 3 using use VBG 10_1101-2020_11_24_390039 411 4 ER ER NNP 10_1101-2020_11_24_390039 411 5 microsomes microsome NNS 10_1101-2020_11_24_390039 411 6 , , , 10_1101-2020_11_24_390039 411 7 Related related JJ 10_1101-2020_11_24_390039 411 8 to to IN 10_1101-2020_11_24_390039 411 9 Figures Figures NNP 10_1101-2020_11_24_390039 411 10 2 2 CD 10_1101-2020_11_24_390039 411 11 and and CC 10_1101-2020_11_24_390039 411 12 3 3 CD 10_1101-2020_11_24_390039 411 13 . . . 10_1101-2020_11_24_390039 412 1 ( ( -LRB- 10_1101-2020_11_24_390039 412 2 A a DT 10_1101-2020_11_24_390039 412 3 ) ) -RRB- 10_1101-2020_11_24_390039 412 4 Non non JJ 10_1101-2020_11_24_390039 412 5 - - JJ 10_1101-2020_11_24_390039 412 6 tagged tagged JJ 10_1101-2020_11_24_390039 412 7 ( ( -LRB- 10_1101-2020_11_24_390039 412 8 lanes lanes NNP 10_1101-2020_11_24_390039 412 9 1 1 CD 10_1101-2020_11_24_390039 412 10 - - SYM 10_1101-2020_11_24_390039 412 11 3 3 CD 10_1101-2020_11_24_390039 412 12 ) ) -RRB- 10_1101-2020_11_24_390039 412 13 and and CC 10_1101-2020_11_24_390039 412 14 OPG2-tagged OPG2-tagged NNP 10_1101-2020_11_24_390039 412 15 ( ( -LRB- 10_1101-2020_11_24_390039 412 16 lanes lanes NNP 10_1101-2020_11_24_390039 412 17 4 4 CD 10_1101-2020_11_24_390039 412 18 - - SYM 10_1101-2020_11_24_390039 412 19 6 6 CD 10_1101-2020_11_24_390039 412 20 ) ) -RRB- 10_1101-2020_11_24_390039 412 21 versions version NNS 10_1101-2020_11_24_390039 412 22 of of IN 10_1101-2020_11_24_390039 412 23 the the DT 10_1101-2020_11_24_390039 412 24 SARS SARS NNP 10_1101-2020_11_24_390039 412 25 - - HYPH 10_1101-2020_11_24_390039 412 26 CoV- CoV- NNP 10_1101-2020_11_24_390039 412 27 2 2 CD 10_1101-2020_11_24_390039 412 28 spike spike JJ 10_1101-2020_11_24_390039 412 29 protein protein NN 10_1101-2020_11_24_390039 412 30 ( ( -LRB- 10_1101-2020_11_24_390039 412 31 S S NNP 10_1101-2020_11_24_390039 412 32 , , , 10_1101-2020_11_24_390039 412 33 S S NNP 10_1101-2020_11_24_390039 412 34 - - HYPH 10_1101-2020_11_24_390039 412 35 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 412 36 ) ) -RRB- 10_1101-2020_11_24_390039 412 37 , , , 10_1101-2020_11_24_390039 412 38 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 412 39 ( ( -LRB- 10_1101-2020_11_24_390039 412 40 ORF8 ORF8 NNP 10_1101-2020_11_24_390039 412 41 , , , 10_1101-2020_11_24_390039 412 42 ORF8-OPG2 ORF8-OPG2 NNP 10_1101-2020_11_24_390039 412 43 ) ) -RRB- 10_1101-2020_11_24_390039 412 44 and and CC 10_1101-2020_11_24_390039 412 45 membrane membrane NN 10_1101-2020_11_24_390039 412 46 protein protein NN 10_1101-2020_11_24_390039 412 47 ( ( -LRB- 10_1101-2020_11_24_390039 412 48 M M NNP 10_1101-2020_11_24_390039 412 49 , , , 10_1101-2020_11_24_390039 412 50 M M NNP 10_1101-2020_11_24_390039 412 51 - - HYPH 10_1101-2020_11_24_390039 412 52 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 412 53 ) ) -RRB- 10_1101-2020_11_24_390039 412 54 were be VBD 10_1101-2020_11_24_390039 412 55 synthesised synthesise VBN 10_1101-2020_11_24_390039 412 56 in in IN 10_1101-2020_11_24_390039 412 57 rabbit rabbit NN 10_1101-2020_11_24_390039 412 58 reticulocyte reticulocyte NNP 10_1101-2020_11_24_390039 412 59 lysate lysate NN 10_1101-2020_11_24_390039 412 60 supplemented supplement VBN 10_1101-2020_11_24_390039 412 61 with with IN 10_1101-2020_11_24_390039 412 62 ER- ER- NNP 10_1101-2020_11_24_390039 412 63 derived derive VBD 10_1101-2020_11_24_390039 412 64 canine canine NN 10_1101-2020_11_24_390039 412 65 pancreatic pancreatic JJ 10_1101-2020_11_24_390039 412 66 microsomes microsome NNS 10_1101-2020_11_24_390039 412 67 in in IN 10_1101-2020_11_24_390039 412 68 the the DT 10_1101-2020_11_24_390039 412 69 absence absence NN 10_1101-2020_11_24_390039 412 70 and and CC 10_1101-2020_11_24_390039 412 71 presence presence NN 10_1101-2020_11_24_390039 412 72 of of IN 10_1101-2020_11_24_390039 412 73 Ipom Ipom NNP 10_1101-2020_11_24_390039 412 74 - - HYPH 10_1101-2020_11_24_390039 412 75 F F NNP 10_1101-2020_11_24_390039 412 76 ( ( -LRB- 10_1101-2020_11_24_390039 412 77 lanes lanes NNP 10_1101-2020_11_24_390039 412 78 1 1 CD 10_1101-2020_11_24_390039 412 79 and and CC 10_1101-2020_11_24_390039 412 80 3 3 CD 10_1101-2020_11_24_390039 412 81 ) ) -RRB- 10_1101-2020_11_24_390039 412 82 . . . 10_1101-2020_11_24_390039 413 1 Phosphorimages phosphorimage NNS 10_1101-2020_11_24_390039 413 2 of of IN 10_1101-2020_11_24_390039 413 3 membrane membrane NN 10_1101-2020_11_24_390039 413 4 - - HYPH 10_1101-2020_11_24_390039 413 5 associated associate VBN 10_1101-2020_11_24_390039 413 6 products product NNS 10_1101-2020_11_24_390039 413 7 resolved resolve VBN 10_1101-2020_11_24_390039 413 8 by by IN 10_1101-2020_11_24_390039 413 9 SDS SDS NNP 10_1101-2020_11_24_390039 413 10 - - HYPH 10_1101-2020_11_24_390039 413 11 PAGE page NN 10_1101-2020_11_24_390039 413 12 together together RB 10_1101-2020_11_24_390039 413 13 with with IN 10_1101-2020_11_24_390039 413 14 representative representative JJ 10_1101-2020_11_24_390039 413 15 substrate substrate NN 10_1101-2020_11_24_390039 413 16 outlines outline NNS 10_1101-2020_11_24_390039 413 17 are be VBP 10_1101-2020_11_24_390039 413 18 shown show VBN 10_1101-2020_11_24_390039 413 19 . . . 10_1101-2020_11_24_390039 414 1 N n LS 10_1101-2020_11_24_390039 414 2 - - HYPH 10_1101-2020_11_24_390039 414 3 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 414 4 ( ( -LRB- 10_1101-2020_11_24_390039 414 5 X X NNP 10_1101-2020_11_24_390039 414 6 - - NNP 10_1101-2020_11_24_390039 414 7 Gly gly NN 10_1101-2020_11_24_390039 414 8 ) ) -RRB- 10_1101-2020_11_24_390039 414 9 versus versus IN 10_1101-2020_11_24_390039 414 10 non non JJ 10_1101-2020_11_24_390039 414 11 - - JJ 10_1101-2020_11_24_390039 414 12 N n JJ 10_1101-2020_11_24_390039 414 13 - - HYPH 10_1101-2020_11_24_390039 414 14 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 414 15 ( ( -LRB- 10_1101-2020_11_24_390039 414 16 0Gly 0gly NN 10_1101-2020_11_24_390039 414 17 ) ) -RRB- 10_1101-2020_11_24_390039 414 18 species specie NNS 10_1101-2020_11_24_390039 414 19 were be VBD 10_1101-2020_11_24_390039 414 20 identified identify VBN 10_1101-2020_11_24_390039 414 21 by by IN 10_1101-2020_11_24_390039 414 22 treatment treatment NN 10_1101-2020_11_24_390039 414 23 with with IN 10_1101-2020_11_24_390039 414 24 endoglycosidase endoglycosidase NN 10_1101-2020_11_24_390039 414 25 H h NN 10_1101-2020_11_24_390039 414 26 ( ( -LRB- 10_1101-2020_11_24_390039 414 27 Endo Endo NNP 10_1101-2020_11_24_390039 414 28 H H NNP 10_1101-2020_11_24_390039 414 29 , , , 10_1101-2020_11_24_390039 414 30 lanes lane VBZ 10_1101-2020_11_24_390039 414 31 2 2 CD 10_1101-2020_11_24_390039 414 32 and and CC 10_1101-2020_11_24_390039 414 33 5 5 CD 10_1101-2020_11_24_390039 414 34 ) ) -RRB- 10_1101-2020_11_24_390039 414 35 . . . 10_1101-2020_11_24_390039 415 1 ( ( -LRB- 10_1101-2020_11_24_390039 415 2 B b NN 10_1101-2020_11_24_390039 415 3 ) ) -RRB- 10_1101-2020_11_24_390039 415 4 The the DT 10_1101-2020_11_24_390039 415 5 S S NNP 10_1101-2020_11_24_390039 415 6 protein protein NN 10_1101-2020_11_24_390039 415 7 was be VBD 10_1101-2020_11_24_390039 415 8 synthesised synthesise VBN 10_1101-2020_11_24_390039 415 9 in in IN 10_1101-2020_11_24_390039 415 10 a a DT 10_1101-2020_11_24_390039 415 11 Flexi Flexi NNP 10_1101-2020_11_24_390039 415 12 ® ® NNPS 10_1101-2020_11_24_390039 415 13 rabbit rabbit NN 10_1101-2020_11_24_390039 415 14 reticulocyte reticulocyte NN 10_1101-2020_11_24_390039 415 15 system system NN 10_1101-2020_11_24_390039 415 16 with with IN 10_1101-2020_11_24_390039 415 17 varying vary VBG 10_1101-2020_11_24_390039 415 18 concentrations concentration NNS 10_1101-2020_11_24_390039 415 19 of of IN 10_1101-2020_11_24_390039 415 20 magnesium magnesium NN 10_1101-2020_11_24_390039 415 21 acetate acetate NN 10_1101-2020_11_24_390039 415 22 ( ( -LRB- 10_1101-2020_11_24_390039 415 23 lanes lanes NNP 10_1101-2020_11_24_390039 415 24 1 1 CD 10_1101-2020_11_24_390039 415 25 - - SYM 10_1101-2020_11_24_390039 415 26 5 5 CD 10_1101-2020_11_24_390039 415 27 ) ) -RRB- 10_1101-2020_11_24_390039 415 28 and and CC 10_1101-2020_11_24_390039 415 29 a a DT 10_1101-2020_11_24_390039 415 30 TNT tnt NN 10_1101-2020_11_24_390039 415 31 ® ® . 10_1101-2020_11_24_390039 415 32 Coupled couple VBN 10_1101-2020_11_24_390039 415 33 system system NN 10_1101-2020_11_24_390039 415 34 ( ( -LRB- 10_1101-2020_11_24_390039 415 35 lane lane NNP 10_1101-2020_11_24_390039 415 36 6 6 CD 10_1101-2020_11_24_390039 415 37 ) ) -RRB- 10_1101-2020_11_24_390039 415 38 in in IN 10_1101-2020_11_24_390039 415 39 the the DT 10_1101-2020_11_24_390039 415 40 absence absence NN 10_1101-2020_11_24_390039 415 41 of of IN 10_1101-2020_11_24_390039 415 42 ER ER NNP 10_1101-2020_11_24_390039 415 43 - - HYPH 10_1101-2020_11_24_390039 415 44 derived derive VBN 10_1101-2020_11_24_390039 415 45 .CC .CC : 10_1101-2020_11_24_390039 415 46 - - HYPH 10_1101-2020_11_24_390039 415 47 BY by IN 10_1101-2020_11_24_390039 415 48 - - HYPH 10_1101-2020_11_24_390039 415 49 ND ND NNP 10_1101-2020_11_24_390039 415 50 4.0 4.0 CD 10_1101-2020_11_24_390039 415 51 International international JJ 10_1101-2020_11_24_390039 415 52 licensemade licensemade NN 10_1101-2020_11_24_390039 415 53 available available JJ 10_1101-2020_11_24_390039 415 54 under under IN 10_1101-2020_11_24_390039 415 55 a a DT 10_1101-2020_11_24_390039 415 56 ( ( -LRB- 10_1101-2020_11_24_390039 415 57 which which WDT 10_1101-2020_11_24_390039 415 58 was be VBD 10_1101-2020_11_24_390039 415 59 not not RB 10_1101-2020_11_24_390039 415 60 certified certify VBN 10_1101-2020_11_24_390039 415 61 by by IN 10_1101-2020_11_24_390039 415 62 peer peer NN 10_1101-2020_11_24_390039 415 63 review review NN 10_1101-2020_11_24_390039 415 64 ) ) -RRB- 10_1101-2020_11_24_390039 415 65 is be VBZ 10_1101-2020_11_24_390039 415 66 the the DT 10_1101-2020_11_24_390039 415 67 author author NN 10_1101-2020_11_24_390039 415 68 / / SYM 10_1101-2020_11_24_390039 415 69 funder funder NN 10_1101-2020_11_24_390039 415 70 , , , 10_1101-2020_11_24_390039 415 71 who who WP 10_1101-2020_11_24_390039 415 72 has have VBZ 10_1101-2020_11_24_390039 415 73 granted grant VBN 10_1101-2020_11_24_390039 415 74 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 415 75 a a DT 10_1101-2020_11_24_390039 415 76 license license NN 10_1101-2020_11_24_390039 415 77 to to TO 10_1101-2020_11_24_390039 415 78 display display VB 10_1101-2020_11_24_390039 415 79 the the DT 10_1101-2020_11_24_390039 415 80 preprint preprint NN 10_1101-2020_11_24_390039 415 81 in in IN 10_1101-2020_11_24_390039 415 82 perpetuity perpetuity NN 10_1101-2020_11_24_390039 415 83 . . . 10_1101-2020_11_24_390039 416 1 It -PRON- PRP 10_1101-2020_11_24_390039 416 2 is be VBZ 10_1101-2020_11_24_390039 416 3 The the DT 10_1101-2020_11_24_390039 416 4 copyright copyright NN 10_1101-2020_11_24_390039 416 5 holder holder NN 10_1101-2020_11_24_390039 416 6 for for IN 10_1101-2020_11_24_390039 416 7 this this DT 10_1101-2020_11_24_390039 416 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 416 9 version version NN 10_1101-2020_11_24_390039 416 10 posted post VBD 10_1101-2020_11_24_390039 416 11 January January NNP 10_1101-2020_11_24_390039 416 12 5 5 CD 10_1101-2020_11_24_390039 416 13 , , , 10_1101-2020_11_24_390039 416 14 2021 2021 CD 10_1101-2020_11_24_390039 416 15 . . . 10_1101-2020_11_24_390039 416 16 ; ; : 10_1101-2020_11_24_390039 416 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 416 18 : : : 10_1101-2020_11_24_390039 416 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 416 20 preprint preprint NN 10_1101-2020_11_24_390039 416 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 416 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 416 23 microsomes microsome NNS 10_1101-2020_11_24_390039 416 24 . . . 10_1101-2020_11_24_390039 417 1 5 5 CD 10_1101-2020_11_24_390039 417 2 % % NN 10_1101-2020_11_24_390039 417 3 of of IN 10_1101-2020_11_24_390039 417 4 the the DT 10_1101-2020_11_24_390039 417 5 total total JJ 10_1101-2020_11_24_390039 417 6 reaction reaction NN 10_1101-2020_11_24_390039 417 7 material material NN 10_1101-2020_11_24_390039 417 8 was be VBD 10_1101-2020_11_24_390039 417 9 resolved resolve VBN 10_1101-2020_11_24_390039 417 10 by by IN 10_1101-2020_11_24_390039 417 11 SDS SDS NNP 10_1101-2020_11_24_390039 417 12 - - HYPH 10_1101-2020_11_24_390039 417 13 PAGE PAGE NNP 10_1101-2020_11_24_390039 417 14 and and CC 10_1101-2020_11_24_390039 417 15 visualised visualise VBN 10_1101-2020_11_24_390039 417 16 by by IN 10_1101-2020_11_24_390039 417 17 phosphorimaging phosphorimage VBG 10_1101-2020_11_24_390039 417 18 . . . 10_1101-2020_11_24_390039 418 1 ( ( -LRB- 10_1101-2020_11_24_390039 418 2 C C NNP 10_1101-2020_11_24_390039 418 3 ) ) -RRB- 10_1101-2020_11_24_390039 418 4 The the DT 10_1101-2020_11_24_390039 418 5 ER ER NNP 10_1101-2020_11_24_390039 418 6 import import NN 10_1101-2020_11_24_390039 418 7 of of IN 10_1101-2020_11_24_390039 418 8 truncated truncate VBN 10_1101-2020_11_24_390039 418 9 variants variant NNS 10_1101-2020_11_24_390039 418 10 of of IN 10_1101-2020_11_24_390039 418 11 the the DT 10_1101-2020_11_24_390039 418 12 S S NNP 10_1101-2020_11_24_390039 418 13 protein protein NN 10_1101-2020_11_24_390039 418 14 ( ( -LRB- 10_1101-2020_11_24_390039 418 15 S s NN 10_1101-2020_11_24_390039 418 16 - - HYPH 10_1101-2020_11_24_390039 418 17 short short JJ 10_1101-2020_11_24_390039 418 18 , , , 10_1101-2020_11_24_390039 418 19 S S NNP 10_1101-2020_11_24_390039 418 20 - - HYPH 10_1101-2020_11_24_390039 418 21 s.s.-TMD s.s.-TMD NNP 10_1101-2020_11_24_390039 418 22 , , , 10_1101-2020_11_24_390039 418 23 S s JJ 10_1101-2020_11_24_390039 418 24 - - HYPH 10_1101-2020_11_24_390039 418 25 half half NN 10_1101-2020_11_24_390039 418 26 - - HYPH 10_1101-2020_11_24_390039 418 27 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 418 28 ) ) -RRB- 10_1101-2020_11_24_390039 418 29 was be VBD 10_1101-2020_11_24_390039 418 30 analysed analyse VBN 10_1101-2020_11_24_390039 418 31 as as IN 10_1101-2020_11_24_390039 418 32 described describe VBN 10_1101-2020_11_24_390039 418 33 for for IN 10_1101-2020_11_24_390039 418 34 ( ( -LRB- 10_1101-2020_11_24_390039 418 35 A a NN 10_1101-2020_11_24_390039 418 36 ) ) -RRB- 10_1101-2020_11_24_390039 418 37 . . . 10_1101-2020_11_24_390039 419 1 ( ( -LRB- 10_1101-2020_11_24_390039 419 2 D d NN 10_1101-2020_11_24_390039 419 3 ) ) -RRB- 10_1101-2020_11_24_390039 419 4 The the DT 10_1101-2020_11_24_390039 419 5 membrane membrane NN 10_1101-2020_11_24_390039 419 6 - - HYPH 10_1101-2020_11_24_390039 419 7 associated associate VBN 10_1101-2020_11_24_390039 419 8 products product NNS 10_1101-2020_11_24_390039 419 9 of of IN 10_1101-2020_11_24_390039 419 10 the the DT 10_1101-2020_11_24_390039 419 11 doubly doubly RB 10_1101-2020_11_24_390039 419 12 tagged tag VBN 10_1101-2020_11_24_390039 419 13 form form NN 10_1101-2020_11_24_390039 419 14 of of IN 10_1101-2020_11_24_390039 419 15 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 419 16 ( ( -LRB- 10_1101-2020_11_24_390039 419 17 OPG2- OPG2- NNP 10_1101-2020_11_24_390039 419 18 ORF6-OPG2 ORF6-OPG2 NNP 10_1101-2020_11_24_390039 419 19 ) ) -RRB- 10_1101-2020_11_24_390039 419 20 were be VBD 10_1101-2020_11_24_390039 419 21 synthesised synthesise VBN 10_1101-2020_11_24_390039 419 22 as as IN 10_1101-2020_11_24_390039 419 23 in in IN 10_1101-2020_11_24_390039 419 24 ( ( -LRB- 10_1101-2020_11_24_390039 419 25 A a NN 10_1101-2020_11_24_390039 419 26 ) ) -RRB- 10_1101-2020_11_24_390039 419 27 and and CC 10_1101-2020_11_24_390039 419 28 , , , 10_1101-2020_11_24_390039 419 29 following follow VBG 10_1101-2020_11_24_390039 419 30 treatment treatment NN 10_1101-2020_11_24_390039 419 31 with with IN 10_1101-2020_11_24_390039 419 32 sodium sodium NN 10_1101-2020_11_24_390039 419 33 carbonate carbonate NN 10_1101-2020_11_24_390039 419 34 buffer buffer NN 10_1101-2020_11_24_390039 419 35 and and CC 10_1101-2020_11_24_390039 419 36 centrifugation centrifugation NN 10_1101-2020_11_24_390039 419 37 , , , 10_1101-2020_11_24_390039 419 38 the the DT 10_1101-2020_11_24_390039 419 39 pellet pellet NN 10_1101-2020_11_24_390039 419 40 , , , 10_1101-2020_11_24_390039 419 41 enriched enrich VBN 10_1101-2020_11_24_390039 419 42 for for IN 10_1101-2020_11_24_390039 419 43 membrane membrane NN 10_1101-2020_11_24_390039 419 44 - - HYPH 10_1101-2020_11_24_390039 419 45 integrated integrate VBN 10_1101-2020_11_24_390039 419 46 material material NN 10_1101-2020_11_24_390039 419 47 , , , 10_1101-2020_11_24_390039 419 48 and and CC 10_1101-2020_11_24_390039 419 49 supernatant supernatant JJ 10_1101-2020_11_24_390039 419 50 , , , 10_1101-2020_11_24_390039 419 51 largely largely RB 10_1101-2020_11_24_390039 419 52 containing contain VBG 10_1101-2020_11_24_390039 419 53 peripherally peripherally RB 10_1101-2020_11_24_390039 419 54 membrane membrane NN 10_1101-2020_11_24_390039 419 55 - - HYPH 10_1101-2020_11_24_390039 419 56 associated associate VBN 10_1101-2020_11_24_390039 419 57 material material NN 10_1101-2020_11_24_390039 419 58 , , , 10_1101-2020_11_24_390039 419 59 were be VBD 10_1101-2020_11_24_390039 419 60 analysed analyse VBN 10_1101-2020_11_24_390039 419 61 for for IN 10_1101-2020_11_24_390039 419 62 OPG2-ORF6-OPG2 OPG2-ORF6-OPG2 NNP 10_1101-2020_11_24_390039 419 63 . . . 10_1101-2020_11_24_390039 420 1 ( ( -LRB- 10_1101-2020_11_24_390039 420 2 E e NN 10_1101-2020_11_24_390039 420 3 ) ) -RRB- 10_1101-2020_11_24_390039 420 4 The the DT 10_1101-2020_11_24_390039 420 5 membrane membrane NN 10_1101-2020_11_24_390039 420 6 - - HYPH 10_1101-2020_11_24_390039 420 7 associated associate VBN 10_1101-2020_11_24_390039 420 8 products product NNS 10_1101-2020_11_24_390039 420 9 of of IN 10_1101-2020_11_24_390039 420 10 OPG2-ORF6-OPG2 OPG2-ORF6-OPG2 NNP 10_1101-2020_11_24_390039 420 11 were be VBD 10_1101-2020_11_24_390039 420 12 treated treat VBN 10_1101-2020_11_24_390039 420 13 with with IN 10_1101-2020_11_24_390039 420 14 trypsin trypsin NNP 10_1101-2020_11_24_390039 420 15 in in IN 10_1101-2020_11_24_390039 420 16 the the DT 10_1101-2020_11_24_390039 420 17 absence absence NN 10_1101-2020_11_24_390039 420 18 or or CC 10_1101-2020_11_24_390039 420 19 presence presence NN 10_1101-2020_11_24_390039 420 20 of of IN 10_1101-2020_11_24_390039 420 21 Triton-100 Triton-100 NNS 10_1101-2020_11_24_390039 420 22 ( ( -LRB- 10_1101-2020_11_24_390039 420 23 TX-100 TX-100 NNP 10_1101-2020_11_24_390039 420 24 , , , 10_1101-2020_11_24_390039 420 25 lanes lane VBZ 10_1101-2020_11_24_390039 420 26 2 2 CD 10_1101-2020_11_24_390039 420 27 - - SYM 10_1101-2020_11_24_390039 420 28 3 3 CD 10_1101-2020_11_24_390039 420 29 ) ) -RRB- 10_1101-2020_11_24_390039 420 30 . . . 10_1101-2020_11_24_390039 421 1 1 1 LS 10_1101-2020_11_24_390039 421 2 .CC .CC : 10_1101-2020_11_24_390039 421 3 - - HYPH 10_1101-2020_11_24_390039 421 4 BY by IN 10_1101-2020_11_24_390039 421 5 - - HYPH 10_1101-2020_11_24_390039 421 6 ND ND NNP 10_1101-2020_11_24_390039 421 7 4.0 4.0 CD 10_1101-2020_11_24_390039 421 8 International international JJ 10_1101-2020_11_24_390039 421 9 licensemade licensemade NN 10_1101-2020_11_24_390039 421 10 available available JJ 10_1101-2020_11_24_390039 421 11 under under IN 10_1101-2020_11_24_390039 421 12 a a DT 10_1101-2020_11_24_390039 421 13 ( ( -LRB- 10_1101-2020_11_24_390039 421 14 which which WDT 10_1101-2020_11_24_390039 421 15 was be VBD 10_1101-2020_11_24_390039 421 16 not not RB 10_1101-2020_11_24_390039 421 17 certified certify VBN 10_1101-2020_11_24_390039 421 18 by by IN 10_1101-2020_11_24_390039 421 19 peer peer NN 10_1101-2020_11_24_390039 421 20 review review NN 10_1101-2020_11_24_390039 421 21 ) ) -RRB- 10_1101-2020_11_24_390039 421 22 is be VBZ 10_1101-2020_11_24_390039 421 23 the the DT 10_1101-2020_11_24_390039 421 24 author author NN 10_1101-2020_11_24_390039 421 25 / / SYM 10_1101-2020_11_24_390039 421 26 funder funder NN 10_1101-2020_11_24_390039 421 27 , , , 10_1101-2020_11_24_390039 421 28 who who WP 10_1101-2020_11_24_390039 421 29 has have VBZ 10_1101-2020_11_24_390039 421 30 granted grant VBN 10_1101-2020_11_24_390039 421 31 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 421 32 a a DT 10_1101-2020_11_24_390039 421 33 license license NN 10_1101-2020_11_24_390039 421 34 to to TO 10_1101-2020_11_24_390039 421 35 display display VB 10_1101-2020_11_24_390039 421 36 the the DT 10_1101-2020_11_24_390039 421 37 preprint preprint NN 10_1101-2020_11_24_390039 421 38 in in IN 10_1101-2020_11_24_390039 421 39 perpetuity perpetuity NN 10_1101-2020_11_24_390039 421 40 . . . 10_1101-2020_11_24_390039 422 1 It -PRON- PRP 10_1101-2020_11_24_390039 422 2 is be VBZ 10_1101-2020_11_24_390039 422 3 The the DT 10_1101-2020_11_24_390039 422 4 copyright copyright NN 10_1101-2020_11_24_390039 422 5 holder holder NN 10_1101-2020_11_24_390039 422 6 for for IN 10_1101-2020_11_24_390039 422 7 this this DT 10_1101-2020_11_24_390039 422 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 422 9 version version NN 10_1101-2020_11_24_390039 422 10 posted post VBD 10_1101-2020_11_24_390039 422 11 January January NNP 10_1101-2020_11_24_390039 422 12 5 5 CD 10_1101-2020_11_24_390039 422 13 , , , 10_1101-2020_11_24_390039 422 14 2021 2021 CD 10_1101-2020_11_24_390039 422 15 . . . 10_1101-2020_11_24_390039 422 16 ; ; : 10_1101-2020_11_24_390039 422 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 422 18 : : : 10_1101-2020_11_24_390039 422 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 422 20 preprint preprint JJ 10_1101-2020_11_24_390039 422 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 422 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 422 23 Page page NN 10_1101-2020_11_24_390039 422 24 4 4 CD 10_1101-2020_11_24_390039 422 25 of of IN 10_1101-2020_11_24_390039 422 26 5 5 CD 10_1101-2020_11_24_390039 422 27 Fig Fig NNP 10_1101-2020_11_24_390039 422 28 . . . 10_1101-2020_11_24_390039 423 1 S2 s2 NN 10_1101-2020_11_24_390039 423 2 . . . 10_1101-2020_11_24_390039 424 1 Validation validation NN 10_1101-2020_11_24_390039 424 2 of of IN 10_1101-2020_11_24_390039 424 3 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 424 4 and/or and/or CC 10_1101-2020_11_24_390039 424 5 EMC EMC NNP 10_1101-2020_11_24_390039 424 6 subunit subunit NN 10_1101-2020_11_24_390039 424 7 depletions depletion NNS 10_1101-2020_11_24_390039 424 8 in in IN 10_1101-2020_11_24_390039 424 9 SP SP NNP 10_1101-2020_11_24_390039 424 10 cells cell NNS 10_1101-2020_11_24_390039 424 11 , , , 10_1101-2020_11_24_390039 424 12 Related relate VBN 10_1101-2020_11_24_390039 424 13 to to IN 10_1101-2020_11_24_390039 424 14 Figure figure NN 10_1101-2020_11_24_390039 424 15 3 3 CD 10_1101-2020_11_24_390039 424 16 . . . 10_1101-2020_11_24_390039 425 1 ( ( -LRB- 10_1101-2020_11_24_390039 425 2 A a NN 10_1101-2020_11_24_390039 425 3 ) ) -RRB- 10_1101-2020_11_24_390039 425 4 The the DT 10_1101-2020_11_24_390039 425 5 effects effect NNS 10_1101-2020_11_24_390039 425 6 of of IN 10_1101-2020_11_24_390039 425 7 transfecting transfecte VBG 10_1101-2020_11_24_390039 425 8 HeLa HeLa NNP 10_1101-2020_11_24_390039 425 9 cells cell NNS 10_1101-2020_11_24_390039 425 10 with with IN 10_1101-2020_11_24_390039 425 11 non non JJ 10_1101-2020_11_24_390039 425 12 - - JJ 10_1101-2020_11_24_390039 425 13 targeting targeting JJ 10_1101-2020_11_24_390039 425 14 ( ( -LRB- 10_1101-2020_11_24_390039 425 15 NT NT NNP 10_1101-2020_11_24_390039 425 16 ; ; : 10_1101-2020_11_24_390039 425 17 lane lane NNP 10_1101-2020_11_24_390039 425 18 1 1 CD 10_1101-2020_11_24_390039 425 19 ) ) -RRB- 10_1101-2020_11_24_390039 425 20 , , , 10_1101-2020_11_24_390039 425 21 Sec61D- Sec61D- NNP 10_1101-2020_11_24_390039 425 22 targeting targeting NN 10_1101-2020_11_24_390039 425 23 ( ( -LRB- 10_1101-2020_11_24_390039 425 24 lane lane NNP 10_1101-2020_11_24_390039 425 25 2 2 CD 10_1101-2020_11_24_390039 425 26 ) ) -RRB- 10_1101-2020_11_24_390039 425 27 , , , 10_1101-2020_11_24_390039 425 28 EMC5-targeting emc5-targete VBG 10_1101-2020_11_24_390039 425 29 ( ( -LRB- 10_1101-2020_11_24_390039 425 30 lane lane NNP 10_1101-2020_11_24_390039 425 31 3 3 CD 10_1101-2020_11_24_390039 425 32 ) ) -RRB- 10_1101-2020_11_24_390039 425 33 and and CC 10_1101-2020_11_24_390039 425 34 Sec61D+EMC5-targeting sec61d+emc5-targete VBG 10_1101-2020_11_24_390039 425 35 ( ( -LRB- 10_1101-2020_11_24_390039 425 36 lane lane NNP 10_1101-2020_11_24_390039 425 37 4 4 CD 10_1101-2020_11_24_390039 425 38 ) ) -RRB- 10_1101-2020_11_24_390039 425 39 siRNAs sirnas CD 10_1101-2020_11_24_390039 425 40 were be VBD 10_1101-2020_11_24_390039 425 41 determined determine VBN 10_1101-2020_11_24_390039 425 42 after after IN 10_1101-2020_11_24_390039 425 43 semi semi JJ 10_1101-2020_11_24_390039 425 44 - - NN 10_1101-2020_11_24_390039 425 45 permeabilisation permeabilisation NN 10_1101-2020_11_24_390039 425 46 by by IN 10_1101-2020_11_24_390039 425 47 immunoblotting immunoblotte VBG 10_1101-2020_11_24_390039 425 48 for for IN 10_1101-2020_11_24_390039 425 49 target target NN 10_1101-2020_11_24_390039 425 50 genes gene NNS 10_1101-2020_11_24_390039 425 51 ( ( -LRB- 10_1101-2020_11_24_390039 425 52 Sec61D Sec61D NNP 10_1101-2020_11_24_390039 425 53 , , , 10_1101-2020_11_24_390039 425 54 EMC5 EMC5 NNP 10_1101-2020_11_24_390039 425 55 ) ) -RRB- 10_1101-2020_11_24_390039 425 56 . . . 10_1101-2020_11_24_390039 426 1 Controls control NNS 10_1101-2020_11_24_390039 426 2 to to TO 10_1101-2020_11_24_390039 426 3 assess assess VB 10_1101-2020_11_24_390039 426 4 destabilisation destabilisation NN 10_1101-2020_11_24_390039 426 5 of of IN 10_1101-2020_11_24_390039 426 6 the the DT 10_1101-2020_11_24_390039 426 7 wider wide JJR 10_1101-2020_11_24_390039 426 8 EMC EMC NNP 10_1101-2020_11_24_390039 426 9 complex complex NN 10_1101-2020_11_24_390039 426 10 ( ( -LRB- 10_1101-2020_11_24_390039 426 11 EMC2 EMC2 NNP 10_1101-2020_11_24_390039 426 12 and and CC 10_1101-2020_11_24_390039 426 13 EMC6 EMC6 NNP 10_1101-2020_11_24_390039 426 14 ) ) -RRB- 10_1101-2020_11_24_390039 426 15 , , , 10_1101-2020_11_24_390039 426 16 any any DT 10_1101-2020_11_24_390039 426 17 effect effect NN 10_1101-2020_11_24_390039 426 18 on on IN 10_1101-2020_11_24_390039 426 19 the the DT 10_1101-2020_11_24_390039 426 20 N N NNP 10_1101-2020_11_24_390039 426 21 - - HYPH 10_1101-2020_11_24_390039 426 22 glycosylation glycosylation NN 10_1101-2020_11_24_390039 426 23 machinery machinery NN 10_1101-2020_11_24_390039 426 24 ( ( -LRB- 10_1101-2020_11_24_390039 426 25 the the DT 10_1101-2020_11_24_390039 426 26 ER ER NNP 10_1101-2020_11_24_390039 426 27 - - HYPH 10_1101-2020_11_24_390039 426 28 resident resident NN 10_1101-2020_11_24_390039 426 29 48 48 CD 10_1101-2020_11_24_390039 426 30 kDa kDa NNS 10_1101-2020_11_24_390039 426 31 subunit subunit NN 10_1101-2020_11_24_390039 426 32 of of IN 10_1101-2020_11_24_390039 426 33 the the DT 10_1101-2020_11_24_390039 426 34 oligosaccharyl oligosaccharyl NN 10_1101-2020_11_24_390039 426 35 - - HYPH 10_1101-2020_11_24_390039 426 36 transferase transferase NN 10_1101-2020_11_24_390039 426 37 complex complex NN 10_1101-2020_11_24_390039 426 38 ( ( -LRB- 10_1101-2020_11_24_390039 426 39 OST48 ost48 UH 10_1101-2020_11_24_390039 426 40 ) ) -RRB- 10_1101-2020_11_24_390039 426 41 and and CC 10_1101-2020_11_24_390039 426 42 the the DT 10_1101-2020_11_24_390039 426 43 quantity quantity NN 10_1101-2020_11_24_390039 426 44 of of IN 10_1101-2020_11_24_390039 426 45 SP SP NNP 10_1101-2020_11_24_390039 426 46 cells cell NNS 10_1101-2020_11_24_390039 426 47 used use VBN 10_1101-2020_11_24_390039 426 48 in in IN 10_1101-2020_11_24_390039 426 49 each each DT 10_1101-2020_11_24_390039 426 50 experiment experiment NN 10_1101-2020_11_24_390039 426 51 ( ( -LRB- 10_1101-2020_11_24_390039 426 52 the the DT 10_1101-2020_11_24_390039 426 53 nuclear nuclear JJ 10_1101-2020_11_24_390039 426 54 protein protein NN 10_1101-2020_11_24_390039 426 55 Lamin Lamin NNP 10_1101-2020_11_24_390039 426 56 - - HYPH 10_1101-2020_11_24_390039 426 57 B1 b1 NN 10_1101-2020_11_24_390039 426 58 ( ( -LRB- 10_1101-2020_11_24_390039 426 59 LMNB1 lmnb1 NN 10_1101-2020_11_24_390039 426 60 ) ) -RRB- 10_1101-2020_11_24_390039 426 61 ) ) -RRB- 10_1101-2020_11_24_390039 426 62 , , , 10_1101-2020_11_24_390039 426 63 are be VBP 10_1101-2020_11_24_390039 426 64 also also RB 10_1101-2020_11_24_390039 426 65 shown show VBN 10_1101-2020_11_24_390039 426 66 . . . 10_1101-2020_11_24_390039 427 1 ( ( -LRB- 10_1101-2020_11_24_390039 427 2 B b NN 10_1101-2020_11_24_390039 427 3 ) ) -RRB- 10_1101-2020_11_24_390039 427 4 The the DT 10_1101-2020_11_24_390039 427 5 efficiencies efficiency NNS 10_1101-2020_11_24_390039 427 6 of of IN 10_1101-2020_11_24_390039 427 7 siRNA sirna NN 10_1101-2020_11_24_390039 427 8 - - HYPH 10_1101-2020_11_24_390039 427 9 mediated mediate VBN 10_1101-2020_11_24_390039 427 10 knockdown knockdown NNP 10_1101-2020_11_24_390039 427 11 ( ( -LRB- 10_1101-2020_11_24_390039 427 12 bold bold JJ 10_1101-2020_11_24_390039 427 13 ) ) -RRB- 10_1101-2020_11_24_390039 427 14 were be VBD 10_1101-2020_11_24_390039 427 15 calculated calculate VBN 10_1101-2020_11_24_390039 427 16 as as IN 10_1101-2020_11_24_390039 427 17 a a DT 10_1101-2020_11_24_390039 427 18 proportion proportion NN 10_1101-2020_11_24_390039 427 19 of of IN 10_1101-2020_11_24_390039 427 20 the the DT 10_1101-2020_11_24_390039 427 21 signal signal JJ 10_1101-2020_11_24_390039 427 22 intensity intensity NN 10_1101-2020_11_24_390039 427 23 obtained obtain VBN 10_1101-2020_11_24_390039 427 24 with with IN 10_1101-2020_11_24_390039 427 25 the the DT 10_1101-2020_11_24_390039 427 26 NT NT NNP 10_1101-2020_11_24_390039 427 27 control control NN 10_1101-2020_11_24_390039 427 28 ( ( -LRB- 10_1101-2020_11_24_390039 427 29 set set VBN 10_1101-2020_11_24_390039 427 30 as as IN 10_1101-2020_11_24_390039 427 31 100 100 CD 10_1101-2020_11_24_390039 427 32 % % NN 10_1101-2020_11_24_390039 427 33 ) ) -RRB- 10_1101-2020_11_24_390039 427 34 . . . 10_1101-2020_11_24_390039 428 1 .CC .CC NFP 10_1101-2020_11_24_390039 428 2 - - : 10_1101-2020_11_24_390039 428 3 BY by IN 10_1101-2020_11_24_390039 428 4 - - HYPH 10_1101-2020_11_24_390039 428 5 ND ND NNP 10_1101-2020_11_24_390039 428 6 4.0 4.0 CD 10_1101-2020_11_24_390039 428 7 International international JJ 10_1101-2020_11_24_390039 428 8 licensemade licensemade NN 10_1101-2020_11_24_390039 428 9 available available JJ 10_1101-2020_11_24_390039 428 10 under under IN 10_1101-2020_11_24_390039 428 11 a a DT 10_1101-2020_11_24_390039 428 12 ( ( -LRB- 10_1101-2020_11_24_390039 428 13 which which WDT 10_1101-2020_11_24_390039 428 14 was be VBD 10_1101-2020_11_24_390039 428 15 not not RB 10_1101-2020_11_24_390039 428 16 certified certify VBN 10_1101-2020_11_24_390039 428 17 by by IN 10_1101-2020_11_24_390039 428 18 peer peer NN 10_1101-2020_11_24_390039 428 19 review review NN 10_1101-2020_11_24_390039 428 20 ) ) -RRB- 10_1101-2020_11_24_390039 428 21 is be VBZ 10_1101-2020_11_24_390039 428 22 the the DT 10_1101-2020_11_24_390039 428 23 author author NN 10_1101-2020_11_24_390039 428 24 / / SYM 10_1101-2020_11_24_390039 428 25 funder funder NN 10_1101-2020_11_24_390039 428 26 , , , 10_1101-2020_11_24_390039 428 27 who who WP 10_1101-2020_11_24_390039 428 28 has have VBZ 10_1101-2020_11_24_390039 428 29 granted grant VBN 10_1101-2020_11_24_390039 428 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 428 31 a a DT 10_1101-2020_11_24_390039 428 32 license license NN 10_1101-2020_11_24_390039 428 33 to to TO 10_1101-2020_11_24_390039 428 34 display display VB 10_1101-2020_11_24_390039 428 35 the the DT 10_1101-2020_11_24_390039 428 36 preprint preprint NN 10_1101-2020_11_24_390039 428 37 in in IN 10_1101-2020_11_24_390039 428 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 428 39 . . . 10_1101-2020_11_24_390039 429 1 It -PRON- PRP 10_1101-2020_11_24_390039 429 2 is be VBZ 10_1101-2020_11_24_390039 429 3 The the DT 10_1101-2020_11_24_390039 429 4 copyright copyright NN 10_1101-2020_11_24_390039 429 5 holder holder NN 10_1101-2020_11_24_390039 429 6 for for IN 10_1101-2020_11_24_390039 429 7 this this DT 10_1101-2020_11_24_390039 429 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 429 9 version version NN 10_1101-2020_11_24_390039 429 10 posted post VBD 10_1101-2020_11_24_390039 429 11 January January NNP 10_1101-2020_11_24_390039 429 12 5 5 CD 10_1101-2020_11_24_390039 429 13 , , , 10_1101-2020_11_24_390039 429 14 2021 2021 CD 10_1101-2020_11_24_390039 429 15 . . . 10_1101-2020_11_24_390039 429 16 ; ; : 10_1101-2020_11_24_390039 429 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 429 18 : : : 10_1101-2020_11_24_390039 429 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 429 20 preprint preprint JJ 10_1101-2020_11_24_390039 429 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 429 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NNP 10_1101-2020_11_24_390039 429 23 Quantitations quantitation NNS 10_1101-2020_11_24_390039 429 24 are be VBP 10_1101-2020_11_24_390039 429 25 given give VBN 10_1101-2020_11_24_390039 429 26 as as IN 10_1101-2020_11_24_390039 429 27 mean±s.e.m mean±s.e.m NN 10_1101-2020_11_24_390039 429 28 for for IN 10_1101-2020_11_24_390039 429 29 three three CD 10_1101-2020_11_24_390039 429 30 separate separate JJ 10_1101-2020_11_24_390039 429 31 siRNA sirna NN 10_1101-2020_11_24_390039 429 32 treatments treatment NNS 10_1101-2020_11_24_390039 429 33 ( ( -LRB- 10_1101-2020_11_24_390039 429 34 n=3 n=3 NNP 10_1101-2020_11_24_390039 429 35 ) ) -RRB- 10_1101-2020_11_24_390039 429 36 with with IN 10_1101-2020_11_24_390039 429 37 statistical statistical JJ 10_1101-2020_11_24_390039 429 38 significance significance NN 10_1101-2020_11_24_390039 429 39 of of IN 10_1101-2020_11_24_390039 429 40 siRNA siRNA NNP 10_1101-2020_11_24_390039 429 41 - - HYPH 10_1101-2020_11_24_390039 429 42 mediated mediate VBN 10_1101-2020_11_24_390039 429 43 knockdowns knockdown NNS 10_1101-2020_11_24_390039 429 44 ( ( -LRB- 10_1101-2020_11_24_390039 429 45 two two CD 10_1101-2020_11_24_390039 429 46 - - HYPH 10_1101-2020_11_24_390039 429 47 way way NN 10_1101-2020_11_24_390039 429 48 ANOVA ANOVA NNP 10_1101-2020_11_24_390039 429 49 , , , 10_1101-2020_11_24_390039 429 50 DF DF NNP 10_1101-2020_11_24_390039 429 51 and and CC 10_1101-2020_11_24_390039 429 52 F F NNP 10_1101-2020_11_24_390039 429 53 a a DT 10_1101-2020_11_24_390039 429 54 ) ) -RRB- 10_1101-2020_11_24_390039 429 55 D d NN 10_1101-2020_11_24_390039 429 56 c c NN 10_1101-2020_11_24_390039 429 57 a a NN 10_1101-2020_11_24_390039 429 58 . . . 10_1101-2020_11_24_390039 430 1 Statistical statistical JJ 10_1101-2020_11_24_390039 430 2 significance significance NN 10_1101-2020_11_24_390039 430 3 is be VBZ 10_1101-2020_11_24_390039 430 4 given give VBN 10_1101-2020_11_24_390039 430 5 as as IN 10_1101-2020_11_24_390039 430 6 n.s n.s NNP 10_1101-2020_11_24_390039 430 7 . . NNP 10_1101-2020_11_24_390039 430 8 , , , 10_1101-2020_11_24_390039 430 9 non non JJ 10_1101-2020_11_24_390039 430 10 - - JJ 10_1101-2020_11_24_390039 430 11 significant significant JJ 10_1101-2020_11_24_390039 430 12 > > NN 10_1101-2020_11_24_390039 430 13 0.1 0.1 CD 10_1101-2020_11_24_390039 430 14 ; ; : 10_1101-2020_11_24_390039 430 15 * * NFP 10_1101-2020_11_24_390039 430 16 , , , 10_1101-2020_11_24_390039 430 17 P p NN 10_1101-2020_11_24_390039 430 18 < < XX 10_1101-2020_11_24_390039 430 19 0.05 0.05 XX 10_1101-2020_11_24_390039 430 20 ; ; : 10_1101-2020_11_24_390039 430 21 * * NFP 10_1101-2020_11_24_390039 430 22 * * NFP 10_1101-2020_11_24_390039 430 23 * * NFP 10_1101-2020_11_24_390039 430 24 * * NFP 10_1101-2020_11_24_390039 430 25 , , , 10_1101-2020_11_24_390039 430 26 P p NN 10_1101-2020_11_24_390039 430 27 < < XX 10_1101-2020_11_24_390039 430 28 0.0001 0.0001 CD 10_1101-2020_11_24_390039 430 29 . . . 10_1101-2020_11_24_390039 431 1 ( ( -LRB- 10_1101-2020_11_24_390039 431 2 C c NN 10_1101-2020_11_24_390039 431 3 ) ) -RRB- 10_1101-2020_11_24_390039 431 4 Knockdown Knockdown NNP 10_1101-2020_11_24_390039 431 5 efficiencies efficiency NNS 10_1101-2020_11_24_390039 431 6 ( ( -LRB- 10_1101-2020_11_24_390039 431 7 mean±s.e.m mean±s.e.m NNP 10_1101-2020_11_24_390039 431 8 ) ) -RRB- 10_1101-2020_11_24_390039 431 9 for for IN 10_1101-2020_11_24_390039 431 10 each each DT 10_1101-2020_11_24_390039 431 11 of of IN 10_1101-2020_11_24_390039 431 12 the the DT 10_1101-2020_11_24_390039 431 13 target target NN 10_1101-2020_11_24_390039 431 14 genes gene NNS 10_1101-2020_11_24_390039 431 15 . . . 10_1101-2020_11_24_390039 432 1 ( ( -LRB- 10_1101-2020_11_24_390039 432 2 D d NN 10_1101-2020_11_24_390039 432 3 ) ) -RRB- 10_1101-2020_11_24_390039 432 4 A a DT 10_1101-2020_11_24_390039 432 5 truncated truncate VBN 10_1101-2020_11_24_390039 432 6 variant variant NN 10_1101-2020_11_24_390039 432 7 of of IN 10_1101-2020_11_24_390039 432 8 the the DT 10_1101-2020_11_24_390039 432 9 S S NNP 10_1101-2020_11_24_390039 432 10 protein protein NN 10_1101-2020_11_24_390039 432 11 ( ( -LRB- 10_1101-2020_11_24_390039 432 12 S S NNP 10_1101-2020_11_24_390039 432 13 - - HYPH 10_1101-2020_11_24_390039 432 14 half half NN 10_1101-2020_11_24_390039 432 15 - - HYPH 10_1101-2020_11_24_390039 432 16 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 432 17 ) ) -RRB- 10_1101-2020_11_24_390039 432 18 was be VBD 10_1101-2020_11_24_390039 432 19 synthesised synthesise VBN 10_1101-2020_11_24_390039 432 20 in in IN 10_1101-2020_11_24_390039 432 21 rabbit rabbit NN 10_1101-2020_11_24_390039 432 22 reticulocyte reticulocyte NNP 10_1101-2020_11_24_390039 432 23 lysate lysate NN 10_1101-2020_11_24_390039 432 24 supplemented supplement VBN 10_1101-2020_11_24_390039 432 25 with with IN 10_1101-2020_11_24_390039 432 26 SP SP NNP 10_1101-2020_11_24_390039 432 27 cells cell NNS 10_1101-2020_11_24_390039 432 28 with with IN 10_1101-2020_11_24_390039 432 29 impaired impaired JJ 10_1101-2020_11_24_390039 432 30 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 432 31 complex complex NN 10_1101-2020_11_24_390039 432 32 and/or and/or CC 10_1101-2020_11_24_390039 432 33 EMC EMC NNP 10_1101-2020_11_24_390039 432 34 function function NN 10_1101-2020_11_24_390039 432 35 and and CC 10_1101-2020_11_24_390039 432 36 recovered recover VBN 10_1101-2020_11_24_390039 432 37 by by IN 10_1101-2020_11_24_390039 432 38 immunoprecipitation immunoprecipitation NN 10_1101-2020_11_24_390039 432 39 via via IN 10_1101-2020_11_24_390039 432 40 the the DT 10_1101-2020_11_24_390039 432 41 OPG2 OPG2 NNP 10_1101-2020_11_24_390039 432 42 tag tag NN 10_1101-2020_11_24_390039 432 43 . . . 10_1101-2020_11_24_390039 433 1 Radiolabelled radiolabelle VBN 10_1101-2020_11_24_390039 433 2 products product NNS 10_1101-2020_11_24_390039 433 3 resolved resolve VBN 10_1101-2020_11_24_390039 433 4 by by IN 10_1101-2020_11_24_390039 433 5 SDS SDS NNP 10_1101-2020_11_24_390039 433 6 - - HYPH 10_1101-2020_11_24_390039 433 7 PAGE PAGE NNP 10_1101-2020_11_24_390039 433 8 and and CC 10_1101-2020_11_24_390039 433 9 analysed analyse VBN 10_1101-2020_11_24_390039 433 10 by by IN 10_1101-2020_11_24_390039 433 11 phosphorimaging phosphorimage VBG 10_1101-2020_11_24_390039 433 12 . . . 10_1101-2020_11_24_390039 434 1 N n LS 10_1101-2020_11_24_390039 434 2 - - HYPH 10_1101-2020_11_24_390039 434 3 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 434 4 ( ( -LRB- 10_1101-2020_11_24_390039 434 5 14-Gly 14-gly CD 10_1101-2020_11_24_390039 434 6 ) ) -RRB- 10_1101-2020_11_24_390039 434 7 versus versus IN 10_1101-2020_11_24_390039 434 8 non non JJ 10_1101-2020_11_24_390039 434 9 - - JJ 10_1101-2020_11_24_390039 434 10 N n JJ 10_1101-2020_11_24_390039 434 11 - - HYPH 10_1101-2020_11_24_390039 434 12 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 434 13 ( ( -LRB- 10_1101-2020_11_24_390039 434 14 0Gly 0gly NN 10_1101-2020_11_24_390039 434 15 ) ) -RRB- 10_1101-2020_11_24_390039 434 16 species specie NNS 10_1101-2020_11_24_390039 434 17 were be VBD 10_1101-2020_11_24_390039 434 18 identified identify VBN 10_1101-2020_11_24_390039 434 19 by by IN 10_1101-2020_11_24_390039 434 20 treatment treatment NN 10_1101-2020_11_24_390039 434 21 with with IN 10_1101-2020_11_24_390039 434 22 endoglycosidase endoglycosidase NN 10_1101-2020_11_24_390039 434 23 H h NN 10_1101-2020_11_24_390039 434 24 ( ( -LRB- 10_1101-2020_11_24_390039 434 25 Endo Endo NNP 10_1101-2020_11_24_390039 434 26 H H NNP 10_1101-2020_11_24_390039 434 27 , , , 10_1101-2020_11_24_390039 434 28 lane lane NN 10_1101-2020_11_24_390039 434 29 1 1 CD 10_1101-2020_11_24_390039 434 30 ) ) -RRB- 10_1101-2020_11_24_390039 434 31 . . . 10_1101-2020_11_24_390039 435 1 ( ( -LRB- 10_1101-2020_11_24_390039 435 2 E e NN 10_1101-2020_11_24_390039 435 3 ) ) -RRB- 10_1101-2020_11_24_390039 435 4 Further further JJ 10_1101-2020_11_24_390039 435 5 analysis analysis NN 10_1101-2020_11_24_390039 435 6 of of IN 10_1101-2020_11_24_390039 435 7 the the DT 10_1101-2020_11_24_390039 435 8 data datum NNS 10_1101-2020_11_24_390039 435 9 presented present VBN 10_1101-2020_11_24_390039 435 10 in in IN 10_1101-2020_11_24_390039 435 11 Fig Fig NNP 10_1101-2020_11_24_390039 435 12 . . . 10_1101-2020_11_24_390039 436 1 3E 3E NNP 10_1101-2020_11_24_390039 436 2 of of IN 10_1101-2020_11_24_390039 436 3 the the DT 10_1101-2020_11_24_390039 436 4 main main JJ 10_1101-2020_11_24_390039 436 5 text text NN 10_1101-2020_11_24_390039 436 6 . . . 10_1101-2020_11_24_390039 437 1 Here here RB 10_1101-2020_11_24_390039 437 2 , , , 10_1101-2020_11_24_390039 437 3 the the DT 10_1101-2020_11_24_390039 437 4 ratio ratio NN 10_1101-2020_11_24_390039 437 5 of of IN 10_1101-2020_11_24_390039 437 6 3Gly 3gly CD 10_1101-2020_11_24_390039 437 7 and and CC 10_1101-2020_11_24_390039 437 8 4Gly 4gly CD 10_1101-2020_11_24_390039 437 9 bearing bear VBG 10_1101-2020_11_24_390039 437 10 OPG2-ORF6-OPG2 OPG2-ORF6-OPG2 NNP 10_1101-2020_11_24_390039 437 11 N- n- CD 10_1101-2020_11_24_390039 437 12 glycosylated glycosylate VBN 10_1101-2020_11_24_390039 437 13 species specie NNS 10_1101-2020_11_24_390039 437 14 relative relative JJ 10_1101-2020_11_24_390039 437 15 to to IN 10_1101-2020_11_24_390039 437 16 the the DT 10_1101-2020_11_24_390039 437 17 1Gly 1Gly `` 10_1101-2020_11_24_390039 437 18 species specie NNS 10_1101-2020_11_24_390039 437 19 present present JJ 10_1101-2020_11_24_390039 437 20 in in IN 10_1101-2020_11_24_390039 437 21 the the DT 10_1101-2020_11_24_390039 437 22 same same JJ 10_1101-2020_11_24_390039 437 23 sample sample NN 10_1101-2020_11_24_390039 437 24 was be VBD 10_1101-2020_11_24_390039 437 25 used use VBN 10_1101-2020_11_24_390039 437 26 as as IN 10_1101-2020_11_24_390039 437 27 a a DT 10_1101-2020_11_24_390039 437 28 proxy proxy NN 10_1101-2020_11_24_390039 437 29 to to TO 10_1101-2020_11_24_390039 437 30 estimate estimate VB 10_1101-2020_11_24_390039 437 31 potential potential JJ 10_1101-2020_11_24_390039 437 32 mis mis NN 10_1101-2020_11_24_390039 437 33 - - HYPH 10_1101-2020_11_24_390039 437 34 insertion insertion NN 10_1101-2020_11_24_390039 437 35 of of IN 10_1101-2020_11_24_390039 437 36 the the DT 10_1101-2020_11_24_390039 437 37 ORF6 ORF6 NNP 10_1101-2020_11_24_390039 437 38 protein protein NN 10_1101-2020_11_24_390039 437 39 in in IN 10_1101-2020_11_24_390039 437 40 SP SP NNP 10_1101-2020_11_24_390039 437 41 cells cell NNS 10_1101-2020_11_24_390039 437 42 with with IN 10_1101-2020_11_24_390039 437 43 impaired impaired JJ 10_1101-2020_11_24_390039 437 44 Sec61 Sec61 NNP 10_1101-2020_11_24_390039 437 45 complex complex NN 10_1101-2020_11_24_390039 437 46 and/or and/or CC 10_1101-2020_11_24_390039 437 47 EMC EMC NNP 10_1101-2020_11_24_390039 437 48 function function VBP 10_1101-2020_11_24_390039 437 49 relative relative JJ 10_1101-2020_11_24_390039 437 50 to to IN 10_1101-2020_11_24_390039 437 51 the the DT 10_1101-2020_11_24_390039 437 52 NT NT NNP 10_1101-2020_11_24_390039 437 53 control control NN 10_1101-2020_11_24_390039 437 54 ( ( -LRB- 10_1101-2020_11_24_390039 437 55 set set VBN 10_1101-2020_11_24_390039 437 56 to to IN 10_1101-2020_11_24_390039 437 57 100 100 CD 10_1101-2020_11_24_390039 437 58 % % NN 10_1101-2020_11_24_390039 437 59 efficiency efficiency NN 10_1101-2020_11_24_390039 437 60 ) ) -RRB- 10_1101-2020_11_24_390039 437 61 . . . 10_1101-2020_11_24_390039 438 1 Quantitations quantitation NNS 10_1101-2020_11_24_390039 438 2 are be VBP 10_1101-2020_11_24_390039 438 3 given give VBN 10_1101-2020_11_24_390039 438 4 as as IN 10_1101-2020_11_24_390039 438 5 mean±s.e.m mean±s.e.m NN 10_1101-2020_11_24_390039 438 6 for for IN 10_1101-2020_11_24_390039 438 7 independent independent JJ 10_1101-2020_11_24_390039 438 8 translation translation NN 10_1101-2020_11_24_390039 438 9 reactions reaction NNS 10_1101-2020_11_24_390039 438 10 from from IN 10_1101-2020_11_24_390039 438 11 separate separate JJ 10_1101-2020_11_24_390039 438 12 siRNA sirna NN 10_1101-2020_11_24_390039 438 13 treatments treatment NNS 10_1101-2020_11_24_390039 438 14 performed perform VBN 10_1101-2020_11_24_390039 438 15 in in IN 10_1101-2020_11_24_390039 438 16 triplicate triplicate NN 10_1101-2020_11_24_390039 438 17 ( ( -LRB- 10_1101-2020_11_24_390039 438 18 n=3 n=3 NNP 10_1101-2020_11_24_390039 438 19 ) ) -RRB- 10_1101-2020_11_24_390039 438 20 and and CC 10_1101-2020_11_24_390039 438 21 statistical statistical JJ 10_1101-2020_11_24_390039 438 22 significance significance NN 10_1101-2020_11_24_390039 438 23 ( ( -LRB- 10_1101-2020_11_24_390039 438 24 two two CD 10_1101-2020_11_24_390039 438 25 - - HYPH 10_1101-2020_11_24_390039 438 26 way way NN 10_1101-2020_11_24_390039 438 27 ANOVA ANOVA NNP 10_1101-2020_11_24_390039 438 28 , , , 10_1101-2020_11_24_390039 438 29 DF DF NNP 10_1101-2020_11_24_390039 438 30 and and CC 10_1101-2020_11_24_390039 438 31 F F NNP 10_1101-2020_11_24_390039 438 32 values value NNS 10_1101-2020_11_24_390039 438 33 shown show VBN 10_1101-2020_11_24_390039 438 34 in in IN 10_1101-2020_11_24_390039 438 35 the the DT 10_1101-2020_11_24_390039 438 36 figure figure NN 10_1101-2020_11_24_390039 438 37 ) ) -RRB- 10_1101-2020_11_24_390039 438 38 was be VBD 10_1101-2020_11_24_390039 438 39 determined determine VBN 10_1101-2020_11_24_390039 438 40 D d NN 10_1101-2020_11_24_390039 438 41 c c NNP 10_1101-2020_11_24_390039 438 42 a a NN 10_1101-2020_11_24_390039 438 43 . . . 10_1101-2020_11_24_390039 439 1 S S NNP 10_1101-2020_11_24_390039 439 2 a a DT 10_1101-2020_11_24_390039 439 3 ca ca NN 10_1101-2020_11_24_390039 439 4 ificance ificance NN 10_1101-2020_11_24_390039 439 5 is be VBZ 10_1101-2020_11_24_390039 439 6 given give VBN 10_1101-2020_11_24_390039 439 7 as as IN 10_1101-2020_11_24_390039 439 8 n.s n.s NNP 10_1101-2020_11_24_390039 439 9 . . NNP 10_1101-2020_11_24_390039 439 10 , , , 10_1101-2020_11_24_390039 439 11 non- non- NNP 10_1101-2020_11_24_390039 439 12 significant significant JJ 10_1101-2020_11_24_390039 439 13 > > XX 10_1101-2020_11_24_390039 439 14 0.1 0.1 CD 10_1101-2020_11_24_390039 439 15 ; ; : 10_1101-2020_11_24_390039 439 16 * * NFP 10_1101-2020_11_24_390039 439 17 , , , 10_1101-2020_11_24_390039 439 18 P p NN 10_1101-2020_11_24_390039 439 19 < < XX 10_1101-2020_11_24_390039 439 20 0.05 0.05 XX 10_1101-2020_11_24_390039 439 21 ; ; : 10_1101-2020_11_24_390039 439 22 * * NFP 10_1101-2020_11_24_390039 439 23 * * NFP 10_1101-2020_11_24_390039 439 24 * * NFP 10_1101-2020_11_24_390039 439 25 , , , 10_1101-2020_11_24_390039 439 26 P p NN 10_1101-2020_11_24_390039 439 27 < < XX 10_1101-2020_11_24_390039 439 28 0.001 0.001 CD 10_1101-2020_11_24_390039 439 29 . . . 10_1101-2020_11_24_390039 440 1 .CC .CC NFP 10_1101-2020_11_24_390039 440 2 - - : 10_1101-2020_11_24_390039 440 3 BY by IN 10_1101-2020_11_24_390039 440 4 - - HYPH 10_1101-2020_11_24_390039 440 5 ND ND NNP 10_1101-2020_11_24_390039 440 6 4.0 4.0 CD 10_1101-2020_11_24_390039 440 7 International international JJ 10_1101-2020_11_24_390039 440 8 licensemade licensemade NN 10_1101-2020_11_24_390039 440 9 available available JJ 10_1101-2020_11_24_390039 440 10 under under IN 10_1101-2020_11_24_390039 440 11 a a DT 10_1101-2020_11_24_390039 440 12 ( ( -LRB- 10_1101-2020_11_24_390039 440 13 which which WDT 10_1101-2020_11_24_390039 440 14 was be VBD 10_1101-2020_11_24_390039 440 15 not not RB 10_1101-2020_11_24_390039 440 16 certified certify VBN 10_1101-2020_11_24_390039 440 17 by by IN 10_1101-2020_11_24_390039 440 18 peer peer NN 10_1101-2020_11_24_390039 440 19 review review NN 10_1101-2020_11_24_390039 440 20 ) ) -RRB- 10_1101-2020_11_24_390039 440 21 is be VBZ 10_1101-2020_11_24_390039 440 22 the the DT 10_1101-2020_11_24_390039 440 23 author author NN 10_1101-2020_11_24_390039 440 24 / / SYM 10_1101-2020_11_24_390039 440 25 funder funder NN 10_1101-2020_11_24_390039 440 26 , , , 10_1101-2020_11_24_390039 440 27 who who WP 10_1101-2020_11_24_390039 440 28 has have VBZ 10_1101-2020_11_24_390039 440 29 granted grant VBN 10_1101-2020_11_24_390039 440 30 bioRxiv biorxiv IN 10_1101-2020_11_24_390039 440 31 a a DT 10_1101-2020_11_24_390039 440 32 license license NN 10_1101-2020_11_24_390039 440 33 to to TO 10_1101-2020_11_24_390039 440 34 display display VB 10_1101-2020_11_24_390039 440 35 the the DT 10_1101-2020_11_24_390039 440 36 preprint preprint NN 10_1101-2020_11_24_390039 440 37 in in IN 10_1101-2020_11_24_390039 440 38 perpetuity perpetuity NN 10_1101-2020_11_24_390039 440 39 . . . 10_1101-2020_11_24_390039 441 1 It -PRON- PRP 10_1101-2020_11_24_390039 441 2 is be VBZ 10_1101-2020_11_24_390039 441 3 The the DT 10_1101-2020_11_24_390039 441 4 copyright copyright NN 10_1101-2020_11_24_390039 441 5 holder holder NN 10_1101-2020_11_24_390039 441 6 for for IN 10_1101-2020_11_24_390039 441 7 this this DT 10_1101-2020_11_24_390039 441 8 preprintthis preprintthis NN 10_1101-2020_11_24_390039 441 9 version version NN 10_1101-2020_11_24_390039 441 10 posted post VBD 10_1101-2020_11_24_390039 441 11 January January NNP 10_1101-2020_11_24_390039 441 12 5 5 CD 10_1101-2020_11_24_390039 441 13 , , , 10_1101-2020_11_24_390039 441 14 2021 2021 CD 10_1101-2020_11_24_390039 441 15 . . . 10_1101-2020_11_24_390039 441 16 ; ; : 10_1101-2020_11_24_390039 441 17 https://doi.org/10.1101/2020.11.24.390039doi https://doi.org/10.1101/2020.11.24.390039doi UH 10_1101-2020_11_24_390039 441 18 : : : 10_1101-2020_11_24_390039 441 19 bioRxiv biorxiv VB 10_1101-2020_11_24_390039 441 20 preprint preprint JJ 10_1101-2020_11_24_390039 441 21 https://doi.org/10.1101/2020.11.24.390039 https://doi.org/10.1101/2020.11.24.390039 NN 10_1101-2020_11_24_390039 441 22 http://creativecommons.org/licenses/by-nd/4.0/ http://creativecommons.org/licenses/by-nd/4.0/ NN