id sid tid token lemma pos 10_1101-2021_01_07_425723 1 1 1 1 LS 10_1101-2021_01_07_425723 1 2 Engineering engineer VBG 10_1101-2021_01_07_425723 1 3 the the DT 10_1101-2021_01_07_425723 1 4 thermotolerant thermotolerant JJ 10_1101-2021_01_07_425723 1 5 industrial industrial JJ 10_1101-2021_01_07_425723 1 6 yeast yeast NN 10_1101-2021_01_07_425723 1 7 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 1 8 marxianus marxianus NN 10_1101-2021_01_07_425723 1 9 for for IN 10_1101-2021_01_07_425723 1 10 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 1 11 growth growth NN 10_1101-2021_01_07_425723 1 12 1 1 CD 10_1101-2021_01_07_425723 1 13 Wijbrand Wijbrand NNP 10_1101-2021_01_07_425723 1 14 J. J. NNP 10_1101-2021_01_07_425723 1 15 C. C. NNP 10_1101-2021_01_07_425723 1 16 Dekker Dekker NNP 10_1101-2021_01_07_425723 1 17 , , , 10_1101-2021_01_07_425723 1 18 Raúl Raúl NNP 10_1101-2021_01_07_425723 1 19 A. A. NNP 10_1101-2021_01_07_425723 1 20 Ortiz Ortiz NNP 10_1101-2021_01_07_425723 1 21 - - HYPH 10_1101-2021_01_07_425723 1 22 Merino Merino NNP 10_1101-2021_01_07_425723 1 23 , , , 10_1101-2021_01_07_425723 1 24 Astrid Astrid NNP 10_1101-2021_01_07_425723 1 25 Kaljouw Kaljouw NNP 10_1101-2021_01_07_425723 1 26 , , , 10_1101-2021_01_07_425723 1 27 Julius Julius NNP 10_1101-2021_01_07_425723 1 28 Battjes Battjes NNP 10_1101-2021_01_07_425723 1 29 , , , 10_1101-2021_01_07_425723 1 30 Frank Frank NNP 10_1101-2021_01_07_425723 1 31 W. W. NNP 10_1101-2021_01_07_425723 1 32 Wiering Wiering NNP 10_1101-2021_01_07_425723 1 33 , , , 10_1101-2021_01_07_425723 1 34 Christiaan Christiaan NNP 10_1101-2021_01_07_425723 1 35 2 2 CD 10_1101-2021_01_07_425723 1 36 Mooiman Mooiman NNP 10_1101-2021_01_07_425723 1 37 , , , 10_1101-2021_01_07_425723 1 38 Pilar Pilar NNP 10_1101-2021_01_07_425723 1 39 de de NNP 10_1101-2021_01_07_425723 1 40 la la NNP 10_1101-2021_01_07_425723 1 41 Torre Torre NNP 10_1101-2021_01_07_425723 1 42 , , , 10_1101-2021_01_07_425723 1 43 and and CC 10_1101-2021_01_07_425723 1 44 Jack Jack NNP 10_1101-2021_01_07_425723 1 45 T. T. NNP 10_1101-2021_01_07_425723 1 46 Pronk Pronk NNP 10_1101-2021_01_07_425723 1 47 * * NFP 10_1101-2021_01_07_425723 1 48 3 3 CD 10_1101-2021_01_07_425723 1 49 Department Department NNP 10_1101-2021_01_07_425723 1 50 of of IN 10_1101-2021_01_07_425723 1 51 Biotechnology Biotechnology NNP 10_1101-2021_01_07_425723 1 52 , , , 10_1101-2021_01_07_425723 1 53 Delft Delft NNP 10_1101-2021_01_07_425723 1 54 University University NNP 10_1101-2021_01_07_425723 1 55 of of IN 10_1101-2021_01_07_425723 1 56 Technology Technology NNP 10_1101-2021_01_07_425723 1 57 , , , 10_1101-2021_01_07_425723 1 58 van van NNP 10_1101-2021_01_07_425723 1 59 der der NNP 10_1101-2021_01_07_425723 1 60 Maasweg Maasweg NNP 10_1101-2021_01_07_425723 1 61 9 9 CD 10_1101-2021_01_07_425723 1 62 , , , 10_1101-2021_01_07_425723 1 63 2629 2629 CD 10_1101-2021_01_07_425723 1 64 HZ HZ NNP 10_1101-2021_01_07_425723 1 65 Delft Delft NNP 10_1101-2021_01_07_425723 1 66 , , , 10_1101-2021_01_07_425723 1 67 The the DT 10_1101-2021_01_07_425723 1 68 4 4 CD 10_1101-2021_01_07_425723 1 69 Netherlands Netherlands NNP 10_1101-2021_01_07_425723 1 70 5 5 CD 10_1101-2021_01_07_425723 1 71 * * NFP 10_1101-2021_01_07_425723 1 72 Corresponding Corresponding NNP 10_1101-2021_01_07_425723 1 73 author author NN 10_1101-2021_01_07_425723 1 74 : : : 10_1101-2021_01_07_425723 1 75 Department Department NNP 10_1101-2021_01_07_425723 1 76 of of IN 10_1101-2021_01_07_425723 1 77 Biotechnology Biotechnology NNP 10_1101-2021_01_07_425723 1 78 , , , 10_1101-2021_01_07_425723 1 79 Delft Delft NNP 10_1101-2021_01_07_425723 1 80 University University NNP 10_1101-2021_01_07_425723 1 81 of of IN 10_1101-2021_01_07_425723 1 82 Technology Technology NNP 10_1101-2021_01_07_425723 1 83 , , , 10_1101-2021_01_07_425723 1 84 van van NNP 10_1101-2021_01_07_425723 1 85 der der NNP 10_1101-2021_01_07_425723 1 86 Maasweg Maasweg NNP 10_1101-2021_01_07_425723 1 87 6 6 CD 10_1101-2021_01_07_425723 1 88 9 9 CD 10_1101-2021_01_07_425723 1 89 , , , 10_1101-2021_01_07_425723 1 90 2629 2629 CD 10_1101-2021_01_07_425723 1 91 HZ HZ NNP 10_1101-2021_01_07_425723 1 92 Delft Delft NNP 10_1101-2021_01_07_425723 1 93 , , , 10_1101-2021_01_07_425723 1 94 The the DT 10_1101-2021_01_07_425723 1 95 Netherlands Netherlands NNP 10_1101-2021_01_07_425723 1 96 , , , 10_1101-2021_01_07_425723 1 97 E e NN 10_1101-2021_01_07_425723 1 98 - - NN 10_1101-2021_01_07_425723 1 99 mail mail NN 10_1101-2021_01_07_425723 1 100 : : : 10_1101-2021_01_07_425723 1 101 j.t.pronk@tudelft.nl j.t.pronk@tudelft.nl UH 10_1101-2021_01_07_425723 1 102 , , , 10_1101-2021_01_07_425723 1 103 Tel tel NN 10_1101-2021_01_07_425723 1 104 : : : 10_1101-2021_01_07_425723 1 105 +31 +31 CD 10_1101-2021_01_07_425723 1 106 15 15 CD 10_1101-2021_01_07_425723 1 107 2783214 2783214 CD 10_1101-2021_01_07_425723 1 108 . . . 10_1101-2021_01_07_425723 2 1 7 7 CD 10_1101-2021_01_07_425723 2 2 Wijbrand Wijbrand NNP 10_1101-2021_01_07_425723 2 3 J.C. J.C. NNP 10_1101-2021_01_07_425723 2 4 Dekker Dekker NNP 10_1101-2021_01_07_425723 2 5 w.j.c.dekker@tudelft.nl w.j.c.dekker@tudelft.nl NNP 10_1101-2021_01_07_425723 2 6 8 8 CD 10_1101-2021_01_07_425723 2 7 Raúl Raúl NNP 10_1101-2021_01_07_425723 2 8 A. A. NNP 10_1101-2021_01_07_425723 2 9 Ortiz Ortiz NNP 10_1101-2021_01_07_425723 2 10 - - HYPH 10_1101-2021_01_07_425723 2 11 Merino Merino NNP 10_1101-2021_01_07_425723 2 12 raul.ortiz@tudelft.nl raul.ortiz@tudelft.nl NNS 10_1101-2021_01_07_425723 2 13 https://orcid.org/0000-0003-4186-8941 https://orcid.org/0000-0003-4186-8941 JJ 10_1101-2021_01_07_425723 2 14 9 9 CD 10_1101-2021_01_07_425723 2 15 Astrid Astrid NNP 10_1101-2021_01_07_425723 2 16 Kaljouw Kaljouw NNPS 10_1101-2021_01_07_425723 2 17 astridk20@gmail.com astridk20@gmail.com ADD 10_1101-2021_01_07_425723 2 18 10 10 CD 10_1101-2021_01_07_425723 2 19 Julius Julius NNP 10_1101-2021_01_07_425723 2 20 Battjes Battjes NNP 10_1101-2021_01_07_425723 2 21 juliusbattjes@hotmail.com juliusbattjes@hotmail.com ADD 10_1101-2021_01_07_425723 2 22 11 11 CD 10_1101-2021_01_07_425723 2 23 Frank Frank NNP 10_1101-2021_01_07_425723 2 24 Willem Willem NNP 10_1101-2021_01_07_425723 2 25 Wiering Wiering NNP 10_1101-2021_01_07_425723 2 26 frank.wiering@gmail.com frank.wiering@gmail.com ADD 10_1101-2021_01_07_425723 2 27 12 12 CD 10_1101-2021_01_07_425723 2 28 Christiaan Christiaan NNP 10_1101-2021_01_07_425723 2 29 Mooiman Mooiman NNP 10_1101-2021_01_07_425723 2 30 c.mooiman@tudelft.nl c.mooiman@tudelft.nl NNP 10_1101-2021_01_07_425723 2 31 13 13 CD 10_1101-2021_01_07_425723 2 32 Pilar Pilar NNP 10_1101-2021_01_07_425723 2 33 de de NNP 10_1101-2021_01_07_425723 2 34 la la NNP 10_1101-2021_01_07_425723 2 35 Torre Torre NNP 10_1101-2021_01_07_425723 2 36 pilartocortes@gmail.com pilartocortes@gmail.com NNP 10_1101-2021_01_07_425723 2 37 14 14 CD 10_1101-2021_01_07_425723 2 38 Jack Jack NNP 10_1101-2021_01_07_425723 2 39 T. T. NNP 10_1101-2021_01_07_425723 2 40 Pronk Pronk NNP 10_1101-2021_01_07_425723 2 41 j.t.pronk@tudelft.nl j.t.pronk@tudelft.nl NNP 10_1101-2021_01_07_425723 2 42 https://orcid.org/0000-0002-5617-4611 https://orcid.org/0000-0002-5617-4611 NNP 10_1101-2021_01_07_425723 2 43 15 15 CD 10_1101-2021_01_07_425723 2 44 Manuscript manuscript NN 10_1101-2021_01_07_425723 2 45 for for IN 10_1101-2021_01_07_425723 2 46 submission submission NN 10_1101-2021_01_07_425723 2 47 in in IN 10_1101-2021_01_07_425723 2 48 Nature Nature NNP 10_1101-2021_01_07_425723 2 49 Biotechnology Biotechnology NNP 10_1101-2021_01_07_425723 2 50 , , , 10_1101-2021_01_07_425723 2 51 section section NN 10_1101-2021_01_07_425723 2 52 : : : 10_1101-2021_01_07_425723 2 53 Article article NN 10_1101-2021_01_07_425723 2 54 . . . 10_1101-2021_01_07_425723 3 1 16 16 CD 10_1101-2021_01_07_425723 3 2 .CC .CC : 10_1101-2021_01_07_425723 3 3 - - HYPH 10_1101-2021_01_07_425723 3 4 BY by IN 10_1101-2021_01_07_425723 3 5 - - HYPH 10_1101-2021_01_07_425723 3 6 NC NC NNP 10_1101-2021_01_07_425723 3 7 - - HYPH 10_1101-2021_01_07_425723 3 8 ND ND NNP 10_1101-2021_01_07_425723 3 9 4.0 4.0 CD 10_1101-2021_01_07_425723 3 10 International International NNP 10_1101-2021_01_07_425723 3 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 3 12 under under IN 10_1101-2021_01_07_425723 3 13 a a DT 10_1101-2021_01_07_425723 3 14 ( ( -LRB- 10_1101-2021_01_07_425723 3 15 which which WDT 10_1101-2021_01_07_425723 3 16 was be VBD 10_1101-2021_01_07_425723 3 17 not not RB 10_1101-2021_01_07_425723 3 18 certified certify VBN 10_1101-2021_01_07_425723 3 19 by by IN 10_1101-2021_01_07_425723 3 20 peer peer NN 10_1101-2021_01_07_425723 3 21 review review NN 10_1101-2021_01_07_425723 3 22 ) ) -RRB- 10_1101-2021_01_07_425723 3 23 is be VBZ 10_1101-2021_01_07_425723 3 24 the the DT 10_1101-2021_01_07_425723 3 25 author author NN 10_1101-2021_01_07_425723 3 26 / / SYM 10_1101-2021_01_07_425723 3 27 funder funder NN 10_1101-2021_01_07_425723 3 28 , , , 10_1101-2021_01_07_425723 3 29 who who WP 10_1101-2021_01_07_425723 3 30 has have VBZ 10_1101-2021_01_07_425723 3 31 granted grant VBN 10_1101-2021_01_07_425723 3 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 3 33 a a DT 10_1101-2021_01_07_425723 3 34 license license NN 10_1101-2021_01_07_425723 3 35 to to TO 10_1101-2021_01_07_425723 3 36 display display VB 10_1101-2021_01_07_425723 3 37 the the DT 10_1101-2021_01_07_425723 3 38 preprint preprint NN 10_1101-2021_01_07_425723 3 39 in in IN 10_1101-2021_01_07_425723 3 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 3 41 . . . 10_1101-2021_01_07_425723 4 1 It -PRON- PRP 10_1101-2021_01_07_425723 4 2 is be VBZ 10_1101-2021_01_07_425723 4 3 made make VBN 10_1101-2021_01_07_425723 4 4 The the DT 10_1101-2021_01_07_425723 4 5 copyright copyright NN 10_1101-2021_01_07_425723 4 6 holder holder NN 10_1101-2021_01_07_425723 4 7 for for IN 10_1101-2021_01_07_425723 4 8 this this DT 10_1101-2021_01_07_425723 4 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 4 10 version version NN 10_1101-2021_01_07_425723 4 11 posted post VBD 10_1101-2021_01_07_425723 4 12 January January NNP 10_1101-2021_01_07_425723 4 13 8 8 CD 10_1101-2021_01_07_425723 4 14 , , , 10_1101-2021_01_07_425723 4 15 2021 2021 CD 10_1101-2021_01_07_425723 4 16 . . . 10_1101-2021_01_07_425723 4 17 ; ; : 10_1101-2021_01_07_425723 4 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 4 19 : : : 10_1101-2021_01_07_425723 4 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 4 21 preprint preprint NN 10_1101-2021_01_07_425723 4 22 mailto:j.t.pronk@tudelft.nl mailto:j.t.pronk@tudelft.nl NNP 10_1101-2021_01_07_425723 4 23 mailto:w.j.c.dekker@tudelft.nl mailto:w.j.c.dekker@tudelft.nl NNP 10_1101-2021_01_07_425723 4 24 mailto:raul.ortiz@tudelft.nl mailto:raul.ortiz@tudelft.nl NNP 10_1101-2021_01_07_425723 4 25 https://orcid.org/0000-0003-4186-8941 https://orcid.org/0000-0003-4186-8941 NNP 10_1101-2021_01_07_425723 4 26 mailto:c.mooiman@tudelft.nl mailto:c.mooiman@tudelft.nl NNP 10_1101-2021_01_07_425723 4 27 mailto:j.t.pronk@tudelft.nl mailto:j.t.pronk@tudelft.nl NNP 10_1101-2021_01_07_425723 4 28 https://orcid.org/0000-0002-5617-4611 https://orcid.org/0000-0002-5617-4611 NNP 10_1101-2021_01_07_425723 4 29 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 4 30 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 4 31 2 2 CD 10_1101-2021_01_07_425723 4 32 Abstract Abstract NNP 10_1101-2021_01_07_425723 4 33 17 17 CD 10_1101-2021_01_07_425723 4 34 Current current JJ 10_1101-2021_01_07_425723 4 35 large large JJ 10_1101-2021_01_07_425723 4 36 - - HYPH 10_1101-2021_01_07_425723 4 37 scale scale NN 10_1101-2021_01_07_425723 4 38 , , , 10_1101-2021_01_07_425723 4 39 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 4 40 industrial industrial JJ 10_1101-2021_01_07_425723 4 41 processes process NNS 10_1101-2021_01_07_425723 4 42 for for IN 10_1101-2021_01_07_425723 4 43 ethanol ethanol NN 10_1101-2021_01_07_425723 4 44 production production NN 10_1101-2021_01_07_425723 4 45 from from IN 10_1101-2021_01_07_425723 4 46 renewable renewable JJ 10_1101-2021_01_07_425723 4 47 18 18 CD 10_1101-2021_01_07_425723 4 48 carbohydrates carbohydrate NNS 10_1101-2021_01_07_425723 4 49 predominantly predominantly RB 10_1101-2021_01_07_425723 4 50 rely rely VBP 10_1101-2021_01_07_425723 4 51 on on IN 10_1101-2021_01_07_425723 4 52 the the DT 10_1101-2021_01_07_425723 4 53 mesophilic mesophilic JJ 10_1101-2021_01_07_425723 4 54 yeast yeast NN 10_1101-2021_01_07_425723 4 55 Saccharomyces Saccharomyces NNPS 10_1101-2021_01_07_425723 4 56 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 4 57 . . . 10_1101-2021_01_07_425723 5 1 Use use NN 10_1101-2021_01_07_425723 5 2 of of IN 10_1101-2021_01_07_425723 5 3 19 19 CD 10_1101-2021_01_07_425723 5 4 thermotolerant thermotolerant NN 10_1101-2021_01_07_425723 5 5 , , , 10_1101-2021_01_07_425723 5 6 facultatively facultatively RB 10_1101-2021_01_07_425723 5 7 fermentative fermentative JJ 10_1101-2021_01_07_425723 5 8 yeasts yeast NNS 10_1101-2021_01_07_425723 5 9 such such JJ 10_1101-2021_01_07_425723 5 10 as as IN 10_1101-2021_01_07_425723 5 11 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 5 12 marxianus marxianus NN 10_1101-2021_01_07_425723 5 13 could could MD 10_1101-2021_01_07_425723 5 14 confer confer VB 10_1101-2021_01_07_425723 5 15 20 20 CD 10_1101-2021_01_07_425723 5 16 significant significant JJ 10_1101-2021_01_07_425723 5 17 economic economic JJ 10_1101-2021_01_07_425723 5 18 benefits benefit NNS 10_1101-2021_01_07_425723 5 19 . . . 10_1101-2021_01_07_425723 6 1 However however RB 10_1101-2021_01_07_425723 6 2 , , , 10_1101-2021_01_07_425723 6 3 in in IN 10_1101-2021_01_07_425723 6 4 contrast contrast NN 10_1101-2021_01_07_425723 6 5 to to IN 10_1101-2021_01_07_425723 6 6 S. S. NNP 10_1101-2021_01_07_425723 6 7 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 6 8 , , , 10_1101-2021_01_07_425723 6 9 these these DT 10_1101-2021_01_07_425723 6 10 yeasts yeast NNS 10_1101-2021_01_07_425723 6 11 can can MD 10_1101-2021_01_07_425723 6 12 not not RB 10_1101-2021_01_07_425723 6 13 grow grow VB 10_1101-2021_01_07_425723 6 14 in in IN 10_1101-2021_01_07_425723 6 15 the the DT 10_1101-2021_01_07_425723 6 16 21 21 CD 10_1101-2021_01_07_425723 6 17 absence absence NN 10_1101-2021_01_07_425723 6 18 of of IN 10_1101-2021_01_07_425723 6 19 oxygen oxygen NN 10_1101-2021_01_07_425723 6 20 . . . 10_1101-2021_01_07_425723 7 1 Response response NN 10_1101-2021_01_07_425723 7 2 of of IN 10_1101-2021_01_07_425723 7 3 K. K. NNP 10_1101-2021_01_07_425723 7 4 marxianus marxianus NN 10_1101-2021_01_07_425723 7 5 and and CC 10_1101-2021_01_07_425723 7 6 S. S. NNP 10_1101-2021_01_07_425723 7 7 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 7 8 to to IN 10_1101-2021_01_07_425723 7 9 different different JJ 10_1101-2021_01_07_425723 7 10 oxygen oxygen NN 10_1101-2021_01_07_425723 7 11 - - HYPH 10_1101-2021_01_07_425723 7 12 limitation limitation NN 10_1101-2021_01_07_425723 7 13 regimes regime NNS 10_1101-2021_01_07_425723 7 14 22 22 CD 10_1101-2021_01_07_425723 7 15 were be VBD 10_1101-2021_01_07_425723 7 16 analyzed analyze VBN 10_1101-2021_01_07_425723 7 17 in in IN 10_1101-2021_01_07_425723 7 18 chemostats chemostat NNS 10_1101-2021_01_07_425723 7 19 . . . 10_1101-2021_01_07_425723 8 1 Genome genome JJ 10_1101-2021_01_07_425723 8 2 and and CC 10_1101-2021_01_07_425723 8 3 transcriptome transcriptome DT 10_1101-2021_01_07_425723 8 4 analysis analysis NN 10_1101-2021_01_07_425723 8 5 , , , 10_1101-2021_01_07_425723 8 6 physiological physiological JJ 10_1101-2021_01_07_425723 8 7 responses response NNS 10_1101-2021_01_07_425723 8 8 to to TO 10_1101-2021_01_07_425723 8 9 sterol sterol VB 10_1101-2021_01_07_425723 8 10 23 23 CD 10_1101-2021_01_07_425723 8 11 supplementation supplementation NN 10_1101-2021_01_07_425723 8 12 and and CC 10_1101-2021_01_07_425723 8 13 sterol sterol NN 10_1101-2021_01_07_425723 8 14 - - HYPH 10_1101-2021_01_07_425723 8 15 uptake uptake NN 10_1101-2021_01_07_425723 8 16 measurements measurement NNS 10_1101-2021_01_07_425723 8 17 identified identify VBN 10_1101-2021_01_07_425723 8 18 absence absence NN 10_1101-2021_01_07_425723 8 19 of of IN 10_1101-2021_01_07_425723 8 20 a a DT 10_1101-2021_01_07_425723 8 21 functional functional JJ 10_1101-2021_01_07_425723 8 22 sterol sterol NN 10_1101-2021_01_07_425723 8 23 - - HYPH 10_1101-2021_01_07_425723 8 24 uptake uptake VB 10_1101-2021_01_07_425723 8 25 24 24 CD 10_1101-2021_01_07_425723 8 26 mechanism mechanism NN 10_1101-2021_01_07_425723 8 27 as as IN 10_1101-2021_01_07_425723 8 28 a a DT 10_1101-2021_01_07_425723 8 29 key key JJ 10_1101-2021_01_07_425723 8 30 factor factor NN 10_1101-2021_01_07_425723 8 31 underlying underlie VBG 10_1101-2021_01_07_425723 8 32 the the DT 10_1101-2021_01_07_425723 8 33 oxygen oxygen NN 10_1101-2021_01_07_425723 8 34 requirement requirement NN 10_1101-2021_01_07_425723 8 35 of of IN 10_1101-2021_01_07_425723 8 36 K. K. NNP 10_1101-2021_01_07_425723 8 37 marxianus marxianus NN 10_1101-2021_01_07_425723 8 38 . . . 10_1101-2021_01_07_425723 9 1 Heterologous heterologous JJ 10_1101-2021_01_07_425723 9 2 expression expression NN 10_1101-2021_01_07_425723 9 3 25 25 CD 10_1101-2021_01_07_425723 9 4 of of IN 10_1101-2021_01_07_425723 9 5 a a DT 10_1101-2021_01_07_425723 9 6 squalene squalene NN 10_1101-2021_01_07_425723 9 7 - - HYPH 10_1101-2021_01_07_425723 9 8 tetrahymanol tetrahymanol NN 10_1101-2021_01_07_425723 9 9 cyclase cyclase NN 10_1101-2021_01_07_425723 9 10 enabled enable VBD 10_1101-2021_01_07_425723 9 11 oxygen oxygen NN 10_1101-2021_01_07_425723 9 12 - - HYPH 10_1101-2021_01_07_425723 9 13 independent independent JJ 10_1101-2021_01_07_425723 9 14 synthesis synthesis NN 10_1101-2021_01_07_425723 9 15 of of IN 10_1101-2021_01_07_425723 9 16 the the DT 10_1101-2021_01_07_425723 9 17 sterol sterol NN 10_1101-2021_01_07_425723 9 18 surrogate surrogate NN 10_1101-2021_01_07_425723 9 19 26 26 CD 10_1101-2021_01_07_425723 9 20 tetrahymanol tetrahymanol NNS 10_1101-2021_01_07_425723 9 21 in in IN 10_1101-2021_01_07_425723 9 22 K. K. NNP 10_1101-2021_01_07_425723 9 23 marxianus marxianus NN 10_1101-2021_01_07_425723 9 24 . . . 10_1101-2021_01_07_425723 10 1 After after IN 10_1101-2021_01_07_425723 10 2 a a DT 10_1101-2021_01_07_425723 10 3 brief brief JJ 10_1101-2021_01_07_425723 10 4 adaptation adaptation NN 10_1101-2021_01_07_425723 10 5 under under IN 10_1101-2021_01_07_425723 10 6 oxygen oxygen NN 10_1101-2021_01_07_425723 10 7 - - HYPH 10_1101-2021_01_07_425723 10 8 limited limit VBN 10_1101-2021_01_07_425723 10 9 conditions condition NNS 10_1101-2021_01_07_425723 10 10 , , , 10_1101-2021_01_07_425723 10 11 tetrahymanol-27 tetrahymanol-27 NN 10_1101-2021_01_07_425723 10 12 expressing express VBG 10_1101-2021_01_07_425723 10 13 K. K. NNP 10_1101-2021_01_07_425723 10 14 marxianus marxianus NN 10_1101-2021_01_07_425723 10 15 strains strain NNS 10_1101-2021_01_07_425723 10 16 grew grow VBD 10_1101-2021_01_07_425723 10 17 anaerobically anaerobically RB 10_1101-2021_01_07_425723 10 18 on on IN 10_1101-2021_01_07_425723 10 19 glucose glucose NN 10_1101-2021_01_07_425723 10 20 at at IN 10_1101-2021_01_07_425723 10 21 temperatures temperature NNS 10_1101-2021_01_07_425723 10 22 of of IN 10_1101-2021_01_07_425723 10 23 up up IN 10_1101-2021_01_07_425723 10 24 to to TO 10_1101-2021_01_07_425723 10 25 45 45 CD 10_1101-2021_01_07_425723 10 26 ° ° NNS 10_1101-2021_01_07_425723 10 27 C C NNP 10_1101-2021_01_07_425723 10 28 . . . 10_1101-2021_01_07_425723 11 1 These these DT 10_1101-2021_01_07_425723 11 2 28 28 CD 10_1101-2021_01_07_425723 11 3 results result NNS 10_1101-2021_01_07_425723 11 4 open open VBP 10_1101-2021_01_07_425723 11 5 up up RP 10_1101-2021_01_07_425723 11 6 new new JJ 10_1101-2021_01_07_425723 11 7 directions direction NNS 10_1101-2021_01_07_425723 11 8 in in IN 10_1101-2021_01_07_425723 11 9 the the DT 10_1101-2021_01_07_425723 11 10 development development NN 10_1101-2021_01_07_425723 11 11 of of IN 10_1101-2021_01_07_425723 11 12 thermotolerant thermotolerant JJ 10_1101-2021_01_07_425723 11 13 yeast yeast NN 10_1101-2021_01_07_425723 11 14 strains strain NNS 10_1101-2021_01_07_425723 11 15 for for IN 10_1101-2021_01_07_425723 11 16 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 11 17 29 29 CD 10_1101-2021_01_07_425723 11 18 industrial industrial JJ 10_1101-2021_01_07_425723 11 19 applications application NNS 10_1101-2021_01_07_425723 11 20 . . . 10_1101-2021_01_07_425723 12 1 30 30 CD 10_1101-2021_01_07_425723 12 2 Keywords keyword NNS 10_1101-2021_01_07_425723 12 3 : : : 10_1101-2021_01_07_425723 12 4 Ergosterol ergosterol UH 10_1101-2021_01_07_425723 12 5 , , , 10_1101-2021_01_07_425723 12 6 tetrahymanol tetrahymanol NNS 10_1101-2021_01_07_425723 12 7 , , , 10_1101-2021_01_07_425723 12 8 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 12 9 metabolism metabolism NN 10_1101-2021_01_07_425723 12 10 , , , 10_1101-2021_01_07_425723 12 11 thermotolerance thermotolerance NN 10_1101-2021_01_07_425723 12 12 , , , 10_1101-2021_01_07_425723 12 13 ethanol ethanol NN 10_1101-2021_01_07_425723 12 14 production production NN 10_1101-2021_01_07_425723 12 15 , , , 10_1101-2021_01_07_425723 12 16 31 31 CD 10_1101-2021_01_07_425723 12 17 yeast yeast NN 10_1101-2021_01_07_425723 12 18 biotechnology biotechnology NN 10_1101-2021_01_07_425723 12 19 , , , 10_1101-2021_01_07_425723 12 20 metabolic metabolic NNP 10_1101-2021_01_07_425723 12 21 engineering engineer VBG 10_1101-2021_01_07_425723 12 22 32 32 CD 10_1101-2021_01_07_425723 12 23 .CC .CC , 10_1101-2021_01_07_425723 12 24 - - HYPH 10_1101-2021_01_07_425723 12 25 BY by IN 10_1101-2021_01_07_425723 12 26 - - HYPH 10_1101-2021_01_07_425723 12 27 NC NC NNP 10_1101-2021_01_07_425723 12 28 - - HYPH 10_1101-2021_01_07_425723 12 29 ND ND NNP 10_1101-2021_01_07_425723 12 30 4.0 4.0 CD 10_1101-2021_01_07_425723 12 31 International International NNP 10_1101-2021_01_07_425723 12 32 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 12 33 under under IN 10_1101-2021_01_07_425723 12 34 a a DT 10_1101-2021_01_07_425723 12 35 ( ( -LRB- 10_1101-2021_01_07_425723 12 36 which which WDT 10_1101-2021_01_07_425723 12 37 was be VBD 10_1101-2021_01_07_425723 12 38 not not RB 10_1101-2021_01_07_425723 12 39 certified certify VBN 10_1101-2021_01_07_425723 12 40 by by IN 10_1101-2021_01_07_425723 12 41 peer peer NN 10_1101-2021_01_07_425723 12 42 review review NN 10_1101-2021_01_07_425723 12 43 ) ) -RRB- 10_1101-2021_01_07_425723 12 44 is be VBZ 10_1101-2021_01_07_425723 12 45 the the DT 10_1101-2021_01_07_425723 12 46 author author NN 10_1101-2021_01_07_425723 12 47 / / SYM 10_1101-2021_01_07_425723 12 48 funder funder NN 10_1101-2021_01_07_425723 12 49 , , , 10_1101-2021_01_07_425723 12 50 who who WP 10_1101-2021_01_07_425723 12 51 has have VBZ 10_1101-2021_01_07_425723 12 52 granted grant VBN 10_1101-2021_01_07_425723 12 53 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 12 54 a a DT 10_1101-2021_01_07_425723 12 55 license license NN 10_1101-2021_01_07_425723 12 56 to to TO 10_1101-2021_01_07_425723 12 57 display display VB 10_1101-2021_01_07_425723 12 58 the the DT 10_1101-2021_01_07_425723 12 59 preprint preprint NN 10_1101-2021_01_07_425723 12 60 in in IN 10_1101-2021_01_07_425723 12 61 perpetuity perpetuity NN 10_1101-2021_01_07_425723 12 62 . . . 10_1101-2021_01_07_425723 13 1 It -PRON- PRP 10_1101-2021_01_07_425723 13 2 is be VBZ 10_1101-2021_01_07_425723 13 3 made make VBN 10_1101-2021_01_07_425723 13 4 The the DT 10_1101-2021_01_07_425723 13 5 copyright copyright NN 10_1101-2021_01_07_425723 13 6 holder holder NN 10_1101-2021_01_07_425723 13 7 for for IN 10_1101-2021_01_07_425723 13 8 this this DT 10_1101-2021_01_07_425723 13 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 13 10 version version NN 10_1101-2021_01_07_425723 13 11 posted post VBD 10_1101-2021_01_07_425723 13 12 January January NNP 10_1101-2021_01_07_425723 13 13 8 8 CD 10_1101-2021_01_07_425723 13 14 , , , 10_1101-2021_01_07_425723 13 15 2021 2021 CD 10_1101-2021_01_07_425723 13 16 . . . 10_1101-2021_01_07_425723 13 17 ; ; : 10_1101-2021_01_07_425723 13 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 13 19 : : : 10_1101-2021_01_07_425723 13 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 13 21 preprint preprint NN 10_1101-2021_01_07_425723 13 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 13 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 13 24 3 3 CD 10_1101-2021_01_07_425723 13 25 In in IN 10_1101-2021_01_07_425723 13 26 terms term NNS 10_1101-2021_01_07_425723 13 27 of of IN 10_1101-2021_01_07_425723 13 28 product product NN 10_1101-2021_01_07_425723 13 29 volume volume NN 10_1101-2021_01_07_425723 13 30 ( ( -LRB- 10_1101-2021_01_07_425723 13 31 87 87 CD 10_1101-2021_01_07_425723 13 32 Mton Mton NNP 10_1101-2021_01_07_425723 13 33 y-1)1,2 y-1)1,2 NN 10_1101-2021_01_07_425723 13 34 , , , 10_1101-2021_01_07_425723 13 35 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 13 36 conversion conversion NN 10_1101-2021_01_07_425723 13 37 of of IN 10_1101-2021_01_07_425723 13 38 carbohydrates carbohydrate NNS 10_1101-2021_01_07_425723 13 39 into into IN 10_1101-2021_01_07_425723 13 40 ethanol ethanol NN 10_1101-2021_01_07_425723 13 41 by by IN 10_1101-2021_01_07_425723 13 42 the the DT 10_1101-2021_01_07_425723 13 43 33 33 CD 10_1101-2021_01_07_425723 13 44 yeast yeast NN 10_1101-2021_01_07_425723 13 45 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 13 46 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 13 47 is be VBZ 10_1101-2021_01_07_425723 13 48 the the DT 10_1101-2021_01_07_425723 13 49 single single JJ 10_1101-2021_01_07_425723 13 50 largest large JJS 10_1101-2021_01_07_425723 13 51 process process NN 10_1101-2021_01_07_425723 13 52 in in IN 10_1101-2021_01_07_425723 13 53 industrial industrial JJ 10_1101-2021_01_07_425723 13 54 biotechnology biotechnology NN 10_1101-2021_01_07_425723 13 55 . . . 10_1101-2021_01_07_425723 14 1 For for IN 10_1101-2021_01_07_425723 14 2 34 34 CD 10_1101-2021_01_07_425723 14 3 fermentation fermentation NN 10_1101-2021_01_07_425723 14 4 products product NNS 10_1101-2021_01_07_425723 14 5 such such JJ 10_1101-2021_01_07_425723 14 6 as as IN 10_1101-2021_01_07_425723 14 7 ethanol ethanol NN 10_1101-2021_01_07_425723 14 8 , , , 10_1101-2021_01_07_425723 14 9 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 14 10 process process NN 10_1101-2021_01_07_425723 14 11 conditions condition NNS 10_1101-2021_01_07_425723 14 12 are be VBP 10_1101-2021_01_07_425723 14 13 required require VBN 10_1101-2021_01_07_425723 14 14 to to TO 10_1101-2021_01_07_425723 14 15 maximize maximize VB 10_1101-2021_01_07_425723 14 16 product product NN 10_1101-2021_01_07_425723 14 17 35 35 CD 10_1101-2021_01_07_425723 14 18 yields yield NNS 10_1101-2021_01_07_425723 14 19 and and CC 10_1101-2021_01_07_425723 14 20 to to TO 10_1101-2021_01_07_425723 14 21 minimize minimize VB 10_1101-2021_01_07_425723 14 22 both both DT 10_1101-2021_01_07_425723 14 23 cooling cool VBG 10_1101-2021_01_07_425723 14 24 costs cost NNS 10_1101-2021_01_07_425723 14 25 and and CC 10_1101-2021_01_07_425723 14 26 complexity complexity NN 10_1101-2021_01_07_425723 14 27 of of IN 10_1101-2021_01_07_425723 14 28 bioreactors3 bioreactors3 NN 10_1101-2021_01_07_425723 14 29 . . . 10_1101-2021_01_07_425723 15 1 While while IN 10_1101-2021_01_07_425723 15 2 S. S. NNP 10_1101-2021_01_07_425723 15 3 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 15 4 is be VBZ 10_1101-2021_01_07_425723 15 5 applied apply VBN 10_1101-2021_01_07_425723 15 6 in in IN 10_1101-2021_01_07_425723 15 7 36 36 CD 10_1101-2021_01_07_425723 15 8 many many JJ 10_1101-2021_01_07_425723 15 9 large large JJ 10_1101-2021_01_07_425723 15 10 - - HYPH 10_1101-2021_01_07_425723 15 11 scale scale NN 10_1101-2021_01_07_425723 15 12 processes process NNS 10_1101-2021_01_07_425723 15 13 and and CC 10_1101-2021_01_07_425723 15 14 is be VBZ 10_1101-2021_01_07_425723 15 15 readily readily RB 10_1101-2021_01_07_425723 15 16 accessible accessible JJ 10_1101-2021_01_07_425723 15 17 to to IN 10_1101-2021_01_07_425723 15 18 modern modern JJ 10_1101-2021_01_07_425723 15 19 genome genome NN 10_1101-2021_01_07_425723 15 20 - - HYPH 10_1101-2021_01_07_425723 15 21 editing editing NN 10_1101-2021_01_07_425723 15 22 techniques4,5 techniques4,5 NN 10_1101-2021_01_07_425723 15 23 , , , 10_1101-2021_01_07_425723 15 24 several several JJ 10_1101-2021_01_07_425723 15 25 37 37 CD 10_1101-2021_01_07_425723 15 26 non non NNS 10_1101-2021_01_07_425723 15 27 - - NNS 10_1101-2021_01_07_425723 15 28 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 15 29 yeasts yeast NNS 10_1101-2021_01_07_425723 15 30 have have VBP 10_1101-2021_01_07_425723 15 31 traits trait NNS 10_1101-2021_01_07_425723 15 32 that that WDT 10_1101-2021_01_07_425723 15 33 are be VBP 10_1101-2021_01_07_425723 15 34 attractive attractive JJ 10_1101-2021_01_07_425723 15 35 for for IN 10_1101-2021_01_07_425723 15 36 industrial industrial JJ 10_1101-2021_01_07_425723 15 37 application application NN 10_1101-2021_01_07_425723 15 38 . . . 10_1101-2021_01_07_425723 16 1 In in IN 10_1101-2021_01_07_425723 16 2 particular particular JJ 10_1101-2021_01_07_425723 16 3 , , , 10_1101-2021_01_07_425723 16 4 the the DT 10_1101-2021_01_07_425723 16 5 high high JJ 10_1101-2021_01_07_425723 16 6 38 38 CD 10_1101-2021_01_07_425723 16 7 maximum maximum JJ 10_1101-2021_01_07_425723 16 8 growth growth NN 10_1101-2021_01_07_425723 16 9 temperature temperature NN 10_1101-2021_01_07_425723 16 10 of of IN 10_1101-2021_01_07_425723 16 11 thermotolerant thermotolerant JJ 10_1101-2021_01_07_425723 16 12 yeasts yeast NNS 10_1101-2021_01_07_425723 16 13 , , , 10_1101-2021_01_07_425723 16 14 such such JJ 10_1101-2021_01_07_425723 16 15 as as IN 10_1101-2021_01_07_425723 16 16 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 16 17 marxianus marxianus NN 10_1101-2021_01_07_425723 16 18 ( ( -LRB- 10_1101-2021_01_07_425723 16 19 up up IN 10_1101-2021_01_07_425723 16 20 to to TO 10_1101-2021_01_07_425723 16 21 50 50 CD 10_1101-2021_01_07_425723 16 22 ° ° , 10_1101-2021_01_07_425723 16 23 C C NNP 10_1101-2021_01_07_425723 16 24 39 39 CD 10_1101-2021_01_07_425723 16 25 as as IN 10_1101-2021_01_07_425723 16 26 opposed oppose VBN 10_1101-2021_01_07_425723 16 27 to to IN 10_1101-2021_01_07_425723 16 28 39 39 CD 10_1101-2021_01_07_425723 16 29 ° ° , 10_1101-2021_01_07_425723 16 30 C C NNP 10_1101-2021_01_07_425723 16 31 for for IN 10_1101-2021_01_07_425723 16 32 S. S. NNP 10_1101-2021_01_07_425723 16 33 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 16 34 ) ) -RRB- 10_1101-2021_01_07_425723 16 35 , , , 10_1101-2021_01_07_425723 16 36 could could MD 10_1101-2021_01_07_425723 16 37 enable enable VB 10_1101-2021_01_07_425723 16 38 lower low JJR 10_1101-2021_01_07_425723 16 39 cooling cooling NN 10_1101-2021_01_07_425723 16 40 costs6–8 costs6–8 JJ 10_1101-2021_01_07_425723 16 41 . . . 10_1101-2021_01_07_425723 17 1 Moreover moreover RB 10_1101-2021_01_07_425723 17 2 , , , 10_1101-2021_01_07_425723 17 3 it -PRON- PRP 10_1101-2021_01_07_425723 17 4 could could MD 10_1101-2021_01_07_425723 17 5 reduce reduce VB 10_1101-2021_01_07_425723 17 6 the the DT 10_1101-2021_01_07_425723 17 7 40 40 CD 10_1101-2021_01_07_425723 17 8 required require VBN 10_1101-2021_01_07_425723 17 9 dosage dosage NN 10_1101-2021_01_07_425723 17 10 of of IN 10_1101-2021_01_07_425723 17 11 fungal fungal JJ 10_1101-2021_01_07_425723 17 12 polysaccharide polysaccharide NN 10_1101-2021_01_07_425723 17 13 hydrolases hydrolase NNS 10_1101-2021_01_07_425723 17 14 during during IN 10_1101-2021_01_07_425723 17 15 simultaneous simultaneous JJ 10_1101-2021_01_07_425723 17 16 saccharification saccharification NN 10_1101-2021_01_07_425723 17 17 and and CC 10_1101-2021_01_07_425723 17 18 41 41 CD 10_1101-2021_01_07_425723 17 19 fermentation fermentation NN 10_1101-2021_01_07_425723 17 20 ( ( -LRB- 10_1101-2021_01_07_425723 17 21 SSF ssf NN 10_1101-2021_01_07_425723 17 22 ) ) -RRB- 10_1101-2021_01_07_425723 17 23 processes9,10 processes9,10 UH 10_1101-2021_01_07_425723 17 24 . . . 10_1101-2021_01_07_425723 18 1 However however RB 10_1101-2021_01_07_425723 18 2 , , , 10_1101-2021_01_07_425723 18 3 as as IN 10_1101-2021_01_07_425723 18 4 yet yet RB 10_1101-2021_01_07_425723 18 5 unidentified unidentified JJ 10_1101-2021_01_07_425723 18 6 oxygen oxygen NN 10_1101-2021_01_07_425723 18 7 requirements requirement NNS 10_1101-2021_01_07_425723 18 8 hamper hamper VBP 10_1101-2021_01_07_425723 18 9 42 42 CD 10_1101-2021_01_07_425723 18 10 implementation implementation NN 10_1101-2021_01_07_425723 18 11 of of IN 10_1101-2021_01_07_425723 18 12 K. K. NNP 10_1101-2021_01_07_425723 18 13 marxianus marxianus NN 10_1101-2021_01_07_425723 18 14 in in IN 10_1101-2021_01_07_425723 18 15 large large JJ 10_1101-2021_01_07_425723 18 16 - - HYPH 10_1101-2021_01_07_425723 18 17 scale scale NN 10_1101-2021_01_07_425723 18 18 anaerobic anaerobic NN 10_1101-2021_01_07_425723 18 19 processes11–13 processes11–13 NNP 10_1101-2021_01_07_425723 18 20 . . . 10_1101-2021_01_07_425723 19 1 43 43 CD 10_1101-2021_01_07_425723 19 2 In in IN 10_1101-2021_01_07_425723 19 3 S. S. NNP 10_1101-2021_01_07_425723 19 4 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 19 5 , , , 10_1101-2021_01_07_425723 19 6 fast fast JJ 10_1101-2021_01_07_425723 19 7 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 19 8 growth growth NN 10_1101-2021_01_07_425723 19 9 on on IN 10_1101-2021_01_07_425723 19 10 synthetic synthetic JJ 10_1101-2021_01_07_425723 19 11 media media NN 10_1101-2021_01_07_425723 19 12 requires require VBZ 10_1101-2021_01_07_425723 19 13 supplementation supplementation NN 10_1101-2021_01_07_425723 19 14 with with IN 10_1101-2021_01_07_425723 19 15 a a DT 10_1101-2021_01_07_425723 19 16 source source NN 10_1101-2021_01_07_425723 19 17 of of IN 10_1101-2021_01_07_425723 19 18 44 44 CD 10_1101-2021_01_07_425723 19 19 unsaturated unsaturated JJ 10_1101-2021_01_07_425723 19 20 fatty fatty NN 10_1101-2021_01_07_425723 19 21 acids acid NNS 10_1101-2021_01_07_425723 19 22 ( ( -LRB- 10_1101-2021_01_07_425723 19 23 UFA UFA NNP 10_1101-2021_01_07_425723 19 24 ) ) -RRB- 10_1101-2021_01_07_425723 19 25 , , , 10_1101-2021_01_07_425723 19 26 sterols sterol NNS 10_1101-2021_01_07_425723 19 27 , , , 10_1101-2021_01_07_425723 19 28 as as RB 10_1101-2021_01_07_425723 19 29 well well RB 10_1101-2021_01_07_425723 19 30 as as IN 10_1101-2021_01_07_425723 19 31 several several JJ 10_1101-2021_01_07_425723 19 32 vitamins14–17 vitamins14–17 NN 10_1101-2021_01_07_425723 19 33 . . . 10_1101-2021_01_07_425723 20 1 These these DT 10_1101-2021_01_07_425723 20 2 nutritional nutritional JJ 10_1101-2021_01_07_425723 20 3 requirements requirement NNS 10_1101-2021_01_07_425723 20 4 45 45 CD 10_1101-2021_01_07_425723 20 5 reflect reflect VBP 10_1101-2021_01_07_425723 20 6 well well RB 10_1101-2021_01_07_425723 20 7 - - HYPH 10_1101-2021_01_07_425723 20 8 characterized characterize VBN 10_1101-2021_01_07_425723 20 9 , , , 10_1101-2021_01_07_425723 20 10 oxygen oxygen NN 10_1101-2021_01_07_425723 20 11 - - HYPH 10_1101-2021_01_07_425723 20 12 dependent dependent JJ 10_1101-2021_01_07_425723 20 13 biosynthetic biosynthetic JJ 10_1101-2021_01_07_425723 20 14 reactions reaction NNS 10_1101-2021_01_07_425723 20 15 . . . 10_1101-2021_01_07_425723 21 1 UFA UFA NNP 10_1101-2021_01_07_425723 21 2 synthesis synthesis NN 10_1101-2021_01_07_425723 21 3 involves involve VBZ 10_1101-2021_01_07_425723 21 4 the the DT 10_1101-2021_01_07_425723 21 5 oxygen-46 oxygen-46 NNP 10_1101-2021_01_07_425723 21 6 dependent dependent NNP 10_1101-2021_01_07_425723 21 7 acyl acyl NNP 10_1101-2021_01_07_425723 21 8 - - HYPH 10_1101-2021_01_07_425723 21 9 CoA CoA NNP 10_1101-2021_01_07_425723 21 10 desaturase desaturase NNP 10_1101-2021_01_07_425723 21 11 Ole1 Ole1 NNP 10_1101-2021_01_07_425723 21 12 , , , 10_1101-2021_01_07_425723 21 13 NAD+ NAD+ NNP 10_1101-2021_01_07_425723 21 14 synthesis synthesis NN 10_1101-2021_01_07_425723 21 15 depends depend VBZ 10_1101-2021_01_07_425723 21 16 on on IN 10_1101-2021_01_07_425723 21 17 the the DT 10_1101-2021_01_07_425723 21 18 oxygenases oxygenase NNS 10_1101-2021_01_07_425723 21 19 Bna2 Bna2 NNP 10_1101-2021_01_07_425723 21 20 , , , 10_1101-2021_01_07_425723 21 21 Bna4 bna4 UH 10_1101-2021_01_07_425723 21 22 , , , 10_1101-2021_01_07_425723 21 23 and and CC 10_1101-2021_01_07_425723 21 24 Bna1 Bna1 NNP 10_1101-2021_01_07_425723 21 25 , , , 10_1101-2021_01_07_425723 21 26 47 47 CD 10_1101-2021_01_07_425723 21 27 while while IN 10_1101-2021_01_07_425723 21 28 synthesis synthesis NN 10_1101-2021_01_07_425723 21 29 of of IN 10_1101-2021_01_07_425723 21 30 ergosterol ergosterol NN 10_1101-2021_01_07_425723 21 31 , , , 10_1101-2021_01_07_425723 21 32 the the DT 10_1101-2021_01_07_425723 21 33 main main JJ 10_1101-2021_01_07_425723 21 34 yeast yeast NN 10_1101-2021_01_07_425723 21 35 sterol sterol NN 10_1101-2021_01_07_425723 21 36 , , , 10_1101-2021_01_07_425723 21 37 even even RB 10_1101-2021_01_07_425723 21 38 requires require VBZ 10_1101-2021_01_07_425723 21 39 12 12 CD 10_1101-2021_01_07_425723 21 40 moles mole NNS 10_1101-2021_01_07_425723 21 41 of of IN 10_1101-2021_01_07_425723 21 42 oxygen oxygen NN 10_1101-2021_01_07_425723 21 43 per per IN 10_1101-2021_01_07_425723 21 44 mole mole NN 10_1101-2021_01_07_425723 21 45 . . . 10_1101-2021_01_07_425723 22 1 48 48 CD 10_1101-2021_01_07_425723 22 2 Oxygen oxygen NN 10_1101-2021_01_07_425723 22 3 - - HYPH 10_1101-2021_01_07_425723 22 4 dependent dependent JJ 10_1101-2021_01_07_425723 22 5 reactions reaction NNS 10_1101-2021_01_07_425723 22 6 in in IN 10_1101-2021_01_07_425723 22 7 NAD+ NAD+ NNP 10_1101-2021_01_07_425723 22 8 synthesis synthesis NN 10_1101-2021_01_07_425723 22 9 can can MD 10_1101-2021_01_07_425723 22 10 be be VB 10_1101-2021_01_07_425723 22 11 bypassed bypass VBN 10_1101-2021_01_07_425723 22 12 by by IN 10_1101-2021_01_07_425723 22 13 nutritional nutritional JJ 10_1101-2021_01_07_425723 22 14 supplementation supplementation NN 10_1101-2021_01_07_425723 22 15 of of IN 10_1101-2021_01_07_425723 22 16 49 49 CD 10_1101-2021_01_07_425723 22 17 nicotinic nicotinic JJ 10_1101-2021_01_07_425723 22 18 acid acid NN 10_1101-2021_01_07_425723 22 19 , , , 10_1101-2021_01_07_425723 22 20 which which WDT 10_1101-2021_01_07_425723 22 21 is be VBZ 10_1101-2021_01_07_425723 22 22 a a DT 10_1101-2021_01_07_425723 22 23 standard standard JJ 10_1101-2021_01_07_425723 22 24 ingredient ingredient NN 10_1101-2021_01_07_425723 22 25 of of IN 10_1101-2021_01_07_425723 22 26 synthetic synthetic JJ 10_1101-2021_01_07_425723 22 27 media medium NNS 10_1101-2021_01_07_425723 22 28 for for IN 10_1101-2021_01_07_425723 22 29 cultivation cultivation NN 10_1101-2021_01_07_425723 22 30 of of IN 10_1101-2021_01_07_425723 22 31 S. S. NNP 10_1101-2021_01_07_425723 22 32 cerevisiae17,18 cerevisiae17,18 NNP 10_1101-2021_01_07_425723 22 33 . . . 10_1101-2021_01_07_425723 23 1 50 50 CD 10_1101-2021_01_07_425723 23 2 Ergosterol Ergosterol NNS 10_1101-2021_01_07_425723 23 3 and and CC 10_1101-2021_01_07_425723 23 4 the the DT 10_1101-2021_01_07_425723 23 5 UFA UFA NNP 10_1101-2021_01_07_425723 23 6 source source NN 10_1101-2021_01_07_425723 23 7 Tween Tween NNP 10_1101-2021_01_07_425723 23 8 80 80 CD 10_1101-2021_01_07_425723 23 9 ( ( -LRB- 10_1101-2021_01_07_425723 23 10 polyethoxylated polyethoxylate VBN 10_1101-2021_01_07_425723 23 11 sorbitan sorbitan NNP 10_1101-2021_01_07_425723 23 12 oleate oleate NN 10_1101-2021_01_07_425723 23 13 ) ) -RRB- 10_1101-2021_01_07_425723 23 14 are be VBP 10_1101-2021_01_07_425723 23 15 routinely routinely RB 10_1101-2021_01_07_425723 23 16 included include VBN 10_1101-2021_01_07_425723 23 17 in in IN 10_1101-2021_01_07_425723 23 18 51 51 CD 10_1101-2021_01_07_425723 23 19 media medium NNS 10_1101-2021_01_07_425723 23 20 for for IN 10_1101-2021_01_07_425723 23 21 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 23 22 cultivation cultivation NN 10_1101-2021_01_07_425723 23 23 as as IN 10_1101-2021_01_07_425723 23 24 ‘ ' `` 10_1101-2021_01_07_425723 23 25 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 23 26 growth growth NN 10_1101-2021_01_07_425723 23 27 factors factor NNS 10_1101-2021_01_07_425723 23 28 ’ ' '' 10_1101-2021_01_07_425723 23 29 ( ( -LRB- 10_1101-2021_01_07_425723 23 30 AGF)15,17,19 agf)15,17,19 UH 10_1101-2021_01_07_425723 23 31 . . . 10_1101-2021_01_07_425723 24 1 Under under IN 10_1101-2021_01_07_425723 24 2 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 24 3 conditions condition NNS 10_1101-2021_01_07_425723 24 4 , , , 10_1101-2021_01_07_425723 24 5 S. S. NNP 10_1101-2021_01_07_425723 24 6 52 52 CD 10_1101-2021_01_07_425723 24 7 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 24 8 imports import NNS 10_1101-2021_01_07_425723 24 9 exogenous exogenous JJ 10_1101-2021_01_07_425723 24 10 sterols sterol NNS 10_1101-2021_01_07_425723 24 11 via via IN 10_1101-2021_01_07_425723 24 12 the the DT 10_1101-2021_01_07_425723 24 13 ABC ABC NNP 10_1101-2021_01_07_425723 24 14 transporters transporter NNS 10_1101-2021_01_07_425723 24 15 Aus1 Aus1 NNP 10_1101-2021_01_07_425723 24 16 and and CC 10_1101-2021_01_07_425723 24 17 Pdr1120 Pdr1120 NNP 10_1101-2021_01_07_425723 24 18 . . . 10_1101-2021_01_07_425723 25 1 Mechanisms mechanism NNS 10_1101-2021_01_07_425723 25 2 for for IN 10_1101-2021_01_07_425723 25 3 uptake uptake JJ 10_1101-2021_01_07_425723 25 4 53 53 CD 10_1101-2021_01_07_425723 25 5 and and CC 10_1101-2021_01_07_425723 25 6 hydrolysis hydrolysis NN 10_1101-2021_01_07_425723 25 7 of of IN 10_1101-2021_01_07_425723 25 8 Tween Tween NNP 10_1101-2021_01_07_425723 25 9 80 80 CD 10_1101-2021_01_07_425723 25 10 by by IN 10_1101-2021_01_07_425723 25 11 S. S. NNP 10_1101-2021_01_07_425723 25 12 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 25 13 are be VBP 10_1101-2021_01_07_425723 25 14 unknown unknown JJ 10_1101-2021_01_07_425723 25 15 but but CC 10_1101-2021_01_07_425723 25 16 , , , 10_1101-2021_01_07_425723 25 17 after after IN 10_1101-2021_01_07_425723 25 18 its -PRON- PRP$ 10_1101-2021_01_07_425723 25 19 release release NN 10_1101-2021_01_07_425723 25 20 , , , 10_1101-2021_01_07_425723 25 21 oleate oleate NN 10_1101-2021_01_07_425723 25 22 is be VBZ 10_1101-2021_01_07_425723 25 23 activated activate VBN 10_1101-2021_01_07_425723 25 24 by by IN 10_1101-2021_01_07_425723 25 25 the the DT 10_1101-2021_01_07_425723 25 26 54 54 CD 10_1101-2021_01_07_425723 25 27 acyl acyl NNP 10_1101-2021_01_07_425723 25 28 - - HYPH 10_1101-2021_01_07_425723 25 29 CoA CoA NNP 10_1101-2021_01_07_425723 25 30 synthetases synthetase VBZ 10_1101-2021_01_07_425723 25 31 Faa1 Faa1 NNP 10_1101-2021_01_07_425723 25 32 and and CC 10_1101-2021_01_07_425723 25 33 Faa421,22 Faa421,22 NNP 10_1101-2021_01_07_425723 25 34 . . . 10_1101-2021_01_07_425723 26 1 55 55 CD 10_1101-2021_01_07_425723 26 2 .CC .CC : 10_1101-2021_01_07_425723 26 3 - - HYPH 10_1101-2021_01_07_425723 26 4 BY by IN 10_1101-2021_01_07_425723 26 5 - - HYPH 10_1101-2021_01_07_425723 26 6 NC NC NNP 10_1101-2021_01_07_425723 26 7 - - HYPH 10_1101-2021_01_07_425723 26 8 ND ND NNP 10_1101-2021_01_07_425723 26 9 4.0 4.0 CD 10_1101-2021_01_07_425723 26 10 International International NNP 10_1101-2021_01_07_425723 26 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 26 12 under under IN 10_1101-2021_01_07_425723 26 13 a a DT 10_1101-2021_01_07_425723 26 14 ( ( -LRB- 10_1101-2021_01_07_425723 26 15 which which WDT 10_1101-2021_01_07_425723 26 16 was be VBD 10_1101-2021_01_07_425723 26 17 not not RB 10_1101-2021_01_07_425723 26 18 certified certify VBN 10_1101-2021_01_07_425723 26 19 by by IN 10_1101-2021_01_07_425723 26 20 peer peer NN 10_1101-2021_01_07_425723 26 21 review review NN 10_1101-2021_01_07_425723 26 22 ) ) -RRB- 10_1101-2021_01_07_425723 26 23 is be VBZ 10_1101-2021_01_07_425723 26 24 the the DT 10_1101-2021_01_07_425723 26 25 author author NN 10_1101-2021_01_07_425723 26 26 / / SYM 10_1101-2021_01_07_425723 26 27 funder funder NN 10_1101-2021_01_07_425723 26 28 , , , 10_1101-2021_01_07_425723 26 29 who who WP 10_1101-2021_01_07_425723 26 30 has have VBZ 10_1101-2021_01_07_425723 26 31 granted grant VBN 10_1101-2021_01_07_425723 26 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 26 33 a a DT 10_1101-2021_01_07_425723 26 34 license license NN 10_1101-2021_01_07_425723 26 35 to to TO 10_1101-2021_01_07_425723 26 36 display display VB 10_1101-2021_01_07_425723 26 37 the the DT 10_1101-2021_01_07_425723 26 38 preprint preprint NN 10_1101-2021_01_07_425723 26 39 in in IN 10_1101-2021_01_07_425723 26 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 26 41 . . . 10_1101-2021_01_07_425723 27 1 It -PRON- PRP 10_1101-2021_01_07_425723 27 2 is be VBZ 10_1101-2021_01_07_425723 27 3 made make VBN 10_1101-2021_01_07_425723 27 4 The the DT 10_1101-2021_01_07_425723 27 5 copyright copyright NN 10_1101-2021_01_07_425723 27 6 holder holder NN 10_1101-2021_01_07_425723 27 7 for for IN 10_1101-2021_01_07_425723 27 8 this this DT 10_1101-2021_01_07_425723 27 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 27 10 version version NN 10_1101-2021_01_07_425723 27 11 posted post VBD 10_1101-2021_01_07_425723 27 12 January January NNP 10_1101-2021_01_07_425723 27 13 8 8 CD 10_1101-2021_01_07_425723 27 14 , , , 10_1101-2021_01_07_425723 27 15 2021 2021 CD 10_1101-2021_01_07_425723 27 16 . . . 10_1101-2021_01_07_425723 27 17 ; ; : 10_1101-2021_01_07_425723 27 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 27 19 : : : 10_1101-2021_01_07_425723 27 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 27 21 preprint preprint NN 10_1101-2021_01_07_425723 27 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 27 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 27 24 4 4 CD 10_1101-2021_01_07_425723 27 25 Outside outside IN 10_1101-2021_01_07_425723 27 26 the the DT 10_1101-2021_01_07_425723 27 27 whole whole RB 10_1101-2021_01_07_425723 27 28 - - HYPH 10_1101-2021_01_07_425723 27 29 genome genome NN 10_1101-2021_01_07_425723 27 30 duplicated duplicate VBN 10_1101-2021_01_07_425723 27 31 ( ( -LRB- 10_1101-2021_01_07_425723 27 32 WGD WGD NNP 10_1101-2021_01_07_425723 27 33 ) ) -RRB- 10_1101-2021_01_07_425723 27 34 clade clade NN 10_1101-2021_01_07_425723 27 35 of of IN 10_1101-2021_01_07_425723 27 36 Saccharomycotina Saccharomycotina NNP 10_1101-2021_01_07_425723 27 37 yeasts yeast NNS 10_1101-2021_01_07_425723 27 38 , , , 10_1101-2021_01_07_425723 27 39 only only RB 10_1101-2021_01_07_425723 27 40 few few JJ 10_1101-2021_01_07_425723 27 41 yeasts yeast NNS 10_1101-2021_01_07_425723 27 42 56 56 CD 10_1101-2021_01_07_425723 27 43 ( ( -LRB- 10_1101-2021_01_07_425723 27 44 including include VBG 10_1101-2021_01_07_425723 27 45 Candida Candida NNP 10_1101-2021_01_07_425723 27 46 albicans albicans NNPS 10_1101-2021_01_07_425723 27 47 and and CC 10_1101-2021_01_07_425723 27 48 Brettanomyces Brettanomyces NNPS 10_1101-2021_01_07_425723 27 49 bruxellensis bruxellensis NN 10_1101-2021_01_07_425723 27 50 ) ) -RRB- 10_1101-2021_01_07_425723 27 51 are be VBP 10_1101-2021_01_07_425723 27 52 capable capable JJ 10_1101-2021_01_07_425723 27 53 of of IN 10_1101-2021_01_07_425723 27 54 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 27 55 growth growth NN 10_1101-2021_01_07_425723 27 56 in in IN 10_1101-2021_01_07_425723 27 57 57 57 CD 10_1101-2021_01_07_425723 27 58 synthetic synthetic JJ 10_1101-2021_01_07_425723 27 59 media medium NNS 10_1101-2021_01_07_425723 27 60 supplemented supplement VBN 10_1101-2021_01_07_425723 27 61 with with IN 10_1101-2021_01_07_425723 27 62 vitamins vitamin NNS 10_1101-2021_01_07_425723 27 63 , , , 10_1101-2021_01_07_425723 27 64 ergosterol ergosterol UH 10_1101-2021_01_07_425723 27 65 and and CC 10_1101-2021_01_07_425723 27 66 Tween Tween NNP 10_1101-2021_01_07_425723 27 67 8012,13,23,24 8012,13,23,24 CD 10_1101-2021_01_07_425723 27 68 . . . 10_1101-2021_01_07_425723 28 1 However however RB 10_1101-2021_01_07_425723 28 2 , , , 10_1101-2021_01_07_425723 28 3 most most RBS 10_1101-2021_01_07_425723 28 4 currently currently RB 10_1101-2021_01_07_425723 28 5 58 58 CD 10_1101-2021_01_07_425723 28 6 known know VBN 10_1101-2021_01_07_425723 28 7 yeast yeast NN 10_1101-2021_01_07_425723 28 8 species specie NNS 10_1101-2021_01_07_425723 28 9 readily readily RB 10_1101-2021_01_07_425723 28 10 ferment ferment NN 10_1101-2021_01_07_425723 28 11 glucose glucose NN 10_1101-2021_01_07_425723 28 12 to to IN 10_1101-2021_01_07_425723 28 13 ethanol ethanol NN 10_1101-2021_01_07_425723 28 14 and and CC 10_1101-2021_01_07_425723 28 15 carbon carbon NN 10_1101-2021_01_07_425723 28 16 dioxide dioxide NN 10_1101-2021_01_07_425723 28 17 when when WRB 10_1101-2021_01_07_425723 28 18 exposed expose VBN 10_1101-2021_01_07_425723 28 19 to to IN 10_1101-2021_01_07_425723 28 20 oxygen-59 oxygen-59 JJ 10_1101-2021_01_07_425723 28 21 limited limited JJ 10_1101-2021_01_07_425723 28 22 growth growth NN 10_1101-2021_01_07_425723 28 23 conditions13,25,26 conditions13,25,26 NN 10_1101-2021_01_07_425723 28 24 , , , 10_1101-2021_01_07_425723 28 25 indicating indicate VBG 10_1101-2021_01_07_425723 28 26 that that IN 10_1101-2021_01_07_425723 28 27 they -PRON- PRP 10_1101-2021_01_07_425723 28 28 do do VBP 10_1101-2021_01_07_425723 28 29 not not RB 10_1101-2021_01_07_425723 28 30 depend depend VB 10_1101-2021_01_07_425723 28 31 on on IN 10_1101-2021_01_07_425723 28 32 respiration respiration NN 10_1101-2021_01_07_425723 28 33 for for IN 10_1101-2021_01_07_425723 28 34 energy energy NN 10_1101-2021_01_07_425723 28 35 60 60 CD 10_1101-2021_01_07_425723 28 36 conservation conservation NN 10_1101-2021_01_07_425723 28 37 . . . 10_1101-2021_01_07_425723 29 1 The the DT 10_1101-2021_01_07_425723 29 2 inability inability NN 10_1101-2021_01_07_425723 29 3 of of IN 10_1101-2021_01_07_425723 29 4 the the DT 10_1101-2021_01_07_425723 29 5 large large JJ 10_1101-2021_01_07_425723 29 6 majority majority NN 10_1101-2021_01_07_425723 29 7 of of IN 10_1101-2021_01_07_425723 29 8 facultatively facultatively RB 10_1101-2021_01_07_425723 29 9 fermentative fermentative JJ 10_1101-2021_01_07_425723 29 10 yeast yeast NN 10_1101-2021_01_07_425723 29 11 species specie NNS 10_1101-2021_01_07_425723 29 12 to to TO 10_1101-2021_01_07_425723 29 13 grow grow VB 10_1101-2021_01_07_425723 29 14 61 61 CD 10_1101-2021_01_07_425723 29 15 under under IN 10_1101-2021_01_07_425723 29 16 strictly strictly RB 10_1101-2021_01_07_425723 29 17 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 29 18 conditions condition NNS 10_1101-2021_01_07_425723 29 19 is be VBZ 10_1101-2021_01_07_425723 29 20 therefore therefore RB 10_1101-2021_01_07_425723 29 21 commonly commonly RB 10_1101-2021_01_07_425723 29 22 attributed attribute VBN 10_1101-2021_01_07_425723 29 23 to to IN 10_1101-2021_01_07_425723 29 24 incompletely incompletely RB 10_1101-2021_01_07_425723 29 25 understood understand VBN 10_1101-2021_01_07_425723 29 26 62 62 CD 10_1101-2021_01_07_425723 29 27 oxygen oxygen NN 10_1101-2021_01_07_425723 29 28 requirements requirement NNS 10_1101-2021_01_07_425723 29 29 for for IN 10_1101-2021_01_07_425723 29 30 biosynthetic biosynthetic JJ 10_1101-2021_01_07_425723 29 31 processes11 processes11 NN 10_1101-2021_01_07_425723 29 32 . . . 10_1101-2021_01_07_425723 30 1 Several several JJ 10_1101-2021_01_07_425723 30 2 oxygen oxygen NN 10_1101-2021_01_07_425723 30 3 - - HYPH 10_1101-2021_01_07_425723 30 4 requiring require VBG 10_1101-2021_01_07_425723 30 5 processes process NNS 10_1101-2021_01_07_425723 30 6 have have VBP 10_1101-2021_01_07_425723 30 7 been be VBN 10_1101-2021_01_07_425723 30 8 63 63 CD 10_1101-2021_01_07_425723 30 9 proposed propose VBN 10_1101-2021_01_07_425723 30 10 including include VBG 10_1101-2021_01_07_425723 30 11 involvement involvement NN 10_1101-2021_01_07_425723 30 12 of of IN 10_1101-2021_01_07_425723 30 13 a a DT 10_1101-2021_01_07_425723 30 14 respiration respiration NN 10_1101-2021_01_07_425723 30 15 - - HYPH 10_1101-2021_01_07_425723 30 16 coupled couple VBN 10_1101-2021_01_07_425723 30 17 dihydroorotate dihydroorotate JJ 10_1101-2021_01_07_425723 30 18 dehydrogenase dehydrogenase NN 10_1101-2021_01_07_425723 30 19 in in IN 10_1101-2021_01_07_425723 30 20 pyrimidine pyrimidine JJ 10_1101-2021_01_07_425723 30 21 64 64 CD 10_1101-2021_01_07_425723 30 22 biosynthesis biosynthesis NN 10_1101-2021_01_07_425723 30 23 , , , 10_1101-2021_01_07_425723 30 24 limitations limitation NNS 10_1101-2021_01_07_425723 30 25 in in IN 10_1101-2021_01_07_425723 30 26 uptake uptake JJ 10_1101-2021_01_07_425723 30 27 and/or and/or CC 10_1101-2021_01_07_425723 30 28 metabolism metabolism NN 10_1101-2021_01_07_425723 30 29 of of IN 10_1101-2021_01_07_425723 30 30 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 30 31 growth growth NN 10_1101-2021_01_07_425723 30 32 factors factor NNS 10_1101-2021_01_07_425723 30 33 , , , 10_1101-2021_01_07_425723 30 34 and and CC 10_1101-2021_01_07_425723 30 35 redox redox NN 10_1101-2021_01_07_425723 30 36 - - HYPH 10_1101-2021_01_07_425723 30 37 cofactor cofactor NN 10_1101-2021_01_07_425723 30 38 65 65 CD 10_1101-2021_01_07_425723 30 39 balancing balancing NN 10_1101-2021_01_07_425723 30 40 constraints11,13,27 constraints11,13,27 NN 10_1101-2021_01_07_425723 30 41 . . . 10_1101-2021_01_07_425723 31 1 66 66 CD 10_1101-2021_01_07_425723 31 2 Quantitation quantitation NN 10_1101-2021_01_07_425723 31 3 , , , 10_1101-2021_01_07_425723 31 4 identification identification NN 10_1101-2021_01_07_425723 31 5 and and CC 10_1101-2021_01_07_425723 31 6 elimination elimination NN 10_1101-2021_01_07_425723 31 7 of of IN 10_1101-2021_01_07_425723 31 8 oxygen oxygen NN 10_1101-2021_01_07_425723 31 9 requirements requirement NNS 10_1101-2021_01_07_425723 31 10 in in IN 10_1101-2021_01_07_425723 31 11 non non JJ 10_1101-2021_01_07_425723 31 12 - - JJ 10_1101-2021_01_07_425723 31 13 Saccharomyces saccharomyces JJ 10_1101-2021_01_07_425723 31 14 yeasts yeast NNS 10_1101-2021_01_07_425723 31 15 is be VBZ 10_1101-2021_01_07_425723 31 16 67 67 CD 10_1101-2021_01_07_425723 31 17 hampered hamper VBN 10_1101-2021_01_07_425723 31 18 by by IN 10_1101-2021_01_07_425723 31 19 the the DT 10_1101-2021_01_07_425723 31 20 very very RB 10_1101-2021_01_07_425723 31 21 small small JJ 10_1101-2021_01_07_425723 31 22 amounts amount NNS 10_1101-2021_01_07_425723 31 23 of of IN 10_1101-2021_01_07_425723 31 24 oxygen oxygen NN 10_1101-2021_01_07_425723 31 25 required require VBN 10_1101-2021_01_07_425723 31 26 for for IN 10_1101-2021_01_07_425723 31 27 non non JJ 10_1101-2021_01_07_425723 31 28 - - JJ 10_1101-2021_01_07_425723 31 29 dissimilatory dissimilatory JJ 10_1101-2021_01_07_425723 31 30 purposes purpose NNS 10_1101-2021_01_07_425723 31 31 . . . 10_1101-2021_01_07_425723 32 1 For for IN 10_1101-2021_01_07_425723 32 2 example example NN 10_1101-2021_01_07_425723 32 3 , , , 10_1101-2021_01_07_425723 32 4 68 68 CD 10_1101-2021_01_07_425723 32 5 preventing prevent VBG 10_1101-2021_01_07_425723 32 6 entry entry NN 10_1101-2021_01_07_425723 32 7 of of IN 10_1101-2021_01_07_425723 32 8 the the DT 10_1101-2021_01_07_425723 32 9 small small JJ 10_1101-2021_01_07_425723 32 10 amounts amount NNS 10_1101-2021_01_07_425723 32 11 of of IN 10_1101-2021_01_07_425723 32 12 oxygen oxygen NN 10_1101-2021_01_07_425723 32 13 required require VBN 10_1101-2021_01_07_425723 32 14 for for IN 10_1101-2021_01_07_425723 32 15 sterol sterol NN 10_1101-2021_01_07_425723 32 16 and and CC 10_1101-2021_01_07_425723 32 17 UFA UFA NNP 10_1101-2021_01_07_425723 32 18 synthesis synthesis NN 10_1101-2021_01_07_425723 32 19 in in IN 10_1101-2021_01_07_425723 32 20 laboratory-69 laboratory-69 NN 10_1101-2021_01_07_425723 32 21 scale scale NN 10_1101-2021_01_07_425723 32 22 bioreactor bioreactor NN 10_1101-2021_01_07_425723 32 23 cultures culture NNS 10_1101-2021_01_07_425723 32 24 of of IN 10_1101-2021_01_07_425723 32 25 S. S. NNP 10_1101-2021_01_07_425723 32 26 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 32 27 requires require VBZ 10_1101-2021_01_07_425723 32 28 extreme extreme JJ 10_1101-2021_01_07_425723 32 29 measures measure NNS 10_1101-2021_01_07_425723 32 30 , , , 10_1101-2021_01_07_425723 32 31 such such JJ 10_1101-2021_01_07_425723 32 32 as as IN 10_1101-2021_01_07_425723 32 33 sparging sparge VBG 10_1101-2021_01_07_425723 32 34 with with IN 10_1101-2021_01_07_425723 32 35 ultra ultra JJ 10_1101-2021_01_07_425723 32 36 - - JJ 10_1101-2021_01_07_425723 32 37 pure pure JJ 10_1101-2021_01_07_425723 32 38 70 70 CD 10_1101-2021_01_07_425723 32 39 nitrogen nitrogen NN 10_1101-2021_01_07_425723 32 40 gas gas NN 10_1101-2021_01_07_425723 32 41 and and CC 10_1101-2021_01_07_425723 32 42 use use NN 10_1101-2021_01_07_425723 32 43 of of IN 10_1101-2021_01_07_425723 32 44 tubing tubing NN 10_1101-2021_01_07_425723 32 45 and and CC 10_1101-2021_01_07_425723 32 46 seals seal NNS 10_1101-2021_01_07_425723 32 47 that that WDT 10_1101-2021_01_07_425723 32 48 are be VBP 10_1101-2021_01_07_425723 32 49 resistant resistant JJ 10_1101-2021_01_07_425723 32 50 to to TO 10_1101-2021_01_07_425723 32 51 oxygen oxygen VB 10_1101-2021_01_07_425723 32 52 diffusion25,28 diffusion25,28 JJ 10_1101-2021_01_07_425723 32 53 . . . 10_1101-2021_01_07_425723 33 1 This this DT 10_1101-2021_01_07_425723 33 2 technical technical JJ 10_1101-2021_01_07_425723 33 3 71 71 CD 10_1101-2021_01_07_425723 33 4 challenge challenge NN 10_1101-2021_01_07_425723 33 5 contributes contribute NNS 10_1101-2021_01_07_425723 33 6 to to IN 10_1101-2021_01_07_425723 33 7 conflicting conflicting JJ 10_1101-2021_01_07_425723 33 8 reports report NNS 10_1101-2021_01_07_425723 33 9 on on IN 10_1101-2021_01_07_425723 33 10 the the DT 10_1101-2021_01_07_425723 33 11 ability ability NN 10_1101-2021_01_07_425723 33 12 of of IN 10_1101-2021_01_07_425723 33 13 non non JJ 10_1101-2021_01_07_425723 33 14 - - JJ 10_1101-2021_01_07_425723 33 15 Saccharomyces saccharomyces JJ 10_1101-2021_01_07_425723 33 16 yeasts yeast NNS 10_1101-2021_01_07_425723 33 17 to to TO 10_1101-2021_01_07_425723 33 18 grow grow VB 10_1101-2021_01_07_425723 33 19 72 72 CD 10_1101-2021_01_07_425723 33 20 anaerobically anaerobically RB 10_1101-2021_01_07_425723 33 21 , , , 10_1101-2021_01_07_425723 33 22 as as IN 10_1101-2021_01_07_425723 33 23 exemplified exemplify VBN 10_1101-2021_01_07_425723 33 24 by by IN 10_1101-2021_01_07_425723 33 25 studies study NNS 10_1101-2021_01_07_425723 33 26 on on IN 10_1101-2021_01_07_425723 33 27 the the DT 10_1101-2021_01_07_425723 33 28 thermotolerant thermotolerant JJ 10_1101-2021_01_07_425723 33 29 yeast yeast NN 10_1101-2021_01_07_425723 33 30 K. K. NNP 10_1101-2021_01_07_425723 33 31 marxianus29–31 marxianus29–31 NNP 10_1101-2021_01_07_425723 33 32 . . . 10_1101-2021_01_07_425723 34 1 Paradoxically paradoxically RB 10_1101-2021_01_07_425723 34 2 , , , 10_1101-2021_01_07_425723 34 3 73 73 CD 10_1101-2021_01_07_425723 34 4 the the DT 10_1101-2021_01_07_425723 34 5 same same JJ 10_1101-2021_01_07_425723 34 6 small small JJ 10_1101-2021_01_07_425723 34 7 oxygen oxygen NN 10_1101-2021_01_07_425723 34 8 requirements requirement NNS 10_1101-2021_01_07_425723 34 9 can can MD 10_1101-2021_01_07_425723 34 10 represent represent VB 10_1101-2021_01_07_425723 34 11 a a DT 10_1101-2021_01_07_425723 34 12 real real JJ 10_1101-2021_01_07_425723 34 13 challenge challenge NN 10_1101-2021_01_07_425723 34 14 in in IN 10_1101-2021_01_07_425723 34 15 large large JJ 10_1101-2021_01_07_425723 34 16 - - HYPH 10_1101-2021_01_07_425723 34 17 scale scale NN 10_1101-2021_01_07_425723 34 18 bioreactors bioreactor NNS 10_1101-2021_01_07_425723 34 19 , , , 10_1101-2021_01_07_425723 34 20 in in IN 10_1101-2021_01_07_425723 34 21 which which WDT 10_1101-2021_01_07_425723 34 22 74 74 CD 10_1101-2021_01_07_425723 34 23 oxygen oxygen NN 10_1101-2021_01_07_425723 34 24 availability availability NN 10_1101-2021_01_07_425723 34 25 is be VBZ 10_1101-2021_01_07_425723 34 26 limited limit VBN 10_1101-2021_01_07_425723 34 27 by by IN 10_1101-2021_01_07_425723 34 28 low low JJ 10_1101-2021_01_07_425723 34 29 surface surface NN 10_1101-2021_01_07_425723 34 30 - - HYPH 10_1101-2021_01_07_425723 34 31 to to IN 10_1101-2021_01_07_425723 34 32 - - HYPH 10_1101-2021_01_07_425723 34 33 volume volume NN 10_1101-2021_01_07_425723 34 34 ratios ratio NNS 10_1101-2021_01_07_425723 34 35 and and CC 10_1101-2021_01_07_425723 34 36 vigorous vigorous JJ 10_1101-2021_01_07_425723 34 37 carbon carbon NN 10_1101-2021_01_07_425723 34 38 - - HYPH 10_1101-2021_01_07_425723 34 39 dioxide dioxide NN 10_1101-2021_01_07_425723 34 40 production production NN 10_1101-2021_01_07_425723 34 41 . . . 10_1101-2021_01_07_425723 35 1 75 75 CD 10_1101-2021_01_07_425723 35 2 Identification identification NN 10_1101-2021_01_07_425723 35 3 of of IN 10_1101-2021_01_07_425723 35 4 the the DT 10_1101-2021_01_07_425723 35 5 non non JJ 10_1101-2021_01_07_425723 35 6 - - JJ 10_1101-2021_01_07_425723 35 7 dissimilatory dissimilatory JJ 10_1101-2021_01_07_425723 35 8 oxygen oxygen NN 10_1101-2021_01_07_425723 35 9 requirements requirement NNS 10_1101-2021_01_07_425723 35 10 of of IN 10_1101-2021_01_07_425723 35 11 non non JJ 10_1101-2021_01_07_425723 35 12 - - JJ 10_1101-2021_01_07_425723 35 13 conventional conventional JJ 10_1101-2021_01_07_425723 35 14 yeast yeast NN 10_1101-2021_01_07_425723 35 15 species specie NNS 10_1101-2021_01_07_425723 35 16 is be VBZ 10_1101-2021_01_07_425723 35 17 76 76 CD 10_1101-2021_01_07_425723 35 18 required require VBN 10_1101-2021_01_07_425723 35 19 to to TO 10_1101-2021_01_07_425723 35 20 eliminate eliminate VB 10_1101-2021_01_07_425723 35 21 a a DT 10_1101-2021_01_07_425723 35 22 key key JJ 10_1101-2021_01_07_425723 35 23 bottleneck bottleneck NN 10_1101-2021_01_07_425723 35 24 for for IN 10_1101-2021_01_07_425723 35 25 their -PRON- PRP$ 10_1101-2021_01_07_425723 35 26 application application NN 10_1101-2021_01_07_425723 35 27 in in IN 10_1101-2021_01_07_425723 35 28 industrial industrial JJ 10_1101-2021_01_07_425723 35 29 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 35 30 processes process NNS 10_1101-2021_01_07_425723 35 31 and and CC 10_1101-2021_01_07_425723 35 32 , , , 10_1101-2021_01_07_425723 35 33 on on IN 10_1101-2021_01_07_425723 35 34 a a DT 10_1101-2021_01_07_425723 35 35 77 77 CD 10_1101-2021_01_07_425723 35 36 fundamental fundamental JJ 10_1101-2021_01_07_425723 35 37 level level NN 10_1101-2021_01_07_425723 35 38 , , , 10_1101-2021_01_07_425723 35 39 can can MD 10_1101-2021_01_07_425723 35 40 shed shed VB 10_1101-2021_01_07_425723 35 41 light light NN 10_1101-2021_01_07_425723 35 42 on on IN 10_1101-2021_01_07_425723 35 43 the the DT 10_1101-2021_01_07_425723 35 44 roles role NNS 10_1101-2021_01_07_425723 35 45 of of IN 10_1101-2021_01_07_425723 35 46 oxygen oxygen NN 10_1101-2021_01_07_425723 35 47 in in IN 10_1101-2021_01_07_425723 35 48 eukaryotic eukaryotic JJ 10_1101-2021_01_07_425723 35 49 metabolism metabolism NN 10_1101-2021_01_07_425723 35 50 . . . 10_1101-2021_01_07_425723 36 1 The the DT 10_1101-2021_01_07_425723 36 2 goal goal NN 10_1101-2021_01_07_425723 36 3 of of IN 10_1101-2021_01_07_425723 36 4 this this DT 10_1101-2021_01_07_425723 36 5 study study NN 10_1101-2021_01_07_425723 36 6 78 78 CD 10_1101-2021_01_07_425723 36 7 .CC .CC : 10_1101-2021_01_07_425723 36 8 - - HYPH 10_1101-2021_01_07_425723 36 9 BY by IN 10_1101-2021_01_07_425723 36 10 - - HYPH 10_1101-2021_01_07_425723 36 11 NC NC NNP 10_1101-2021_01_07_425723 36 12 - - HYPH 10_1101-2021_01_07_425723 36 13 ND ND NNP 10_1101-2021_01_07_425723 36 14 4.0 4.0 CD 10_1101-2021_01_07_425723 36 15 International International NNP 10_1101-2021_01_07_425723 36 16 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 36 17 under under IN 10_1101-2021_01_07_425723 36 18 a a DT 10_1101-2021_01_07_425723 36 19 ( ( -LRB- 10_1101-2021_01_07_425723 36 20 which which WDT 10_1101-2021_01_07_425723 36 21 was be VBD 10_1101-2021_01_07_425723 36 22 not not RB 10_1101-2021_01_07_425723 36 23 certified certify VBN 10_1101-2021_01_07_425723 36 24 by by IN 10_1101-2021_01_07_425723 36 25 peer peer NN 10_1101-2021_01_07_425723 36 26 review review NN 10_1101-2021_01_07_425723 36 27 ) ) -RRB- 10_1101-2021_01_07_425723 36 28 is be VBZ 10_1101-2021_01_07_425723 36 29 the the DT 10_1101-2021_01_07_425723 36 30 author author NN 10_1101-2021_01_07_425723 36 31 / / SYM 10_1101-2021_01_07_425723 36 32 funder funder NN 10_1101-2021_01_07_425723 36 33 , , , 10_1101-2021_01_07_425723 36 34 who who WP 10_1101-2021_01_07_425723 36 35 has have VBZ 10_1101-2021_01_07_425723 36 36 granted grant VBN 10_1101-2021_01_07_425723 36 37 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 36 38 a a DT 10_1101-2021_01_07_425723 36 39 license license NN 10_1101-2021_01_07_425723 36 40 to to TO 10_1101-2021_01_07_425723 36 41 display display VB 10_1101-2021_01_07_425723 36 42 the the DT 10_1101-2021_01_07_425723 36 43 preprint preprint NN 10_1101-2021_01_07_425723 36 44 in in IN 10_1101-2021_01_07_425723 36 45 perpetuity perpetuity NN 10_1101-2021_01_07_425723 36 46 . . . 10_1101-2021_01_07_425723 37 1 It -PRON- PRP 10_1101-2021_01_07_425723 37 2 is be VBZ 10_1101-2021_01_07_425723 37 3 made make VBN 10_1101-2021_01_07_425723 37 4 The the DT 10_1101-2021_01_07_425723 37 5 copyright copyright NN 10_1101-2021_01_07_425723 37 6 holder holder NN 10_1101-2021_01_07_425723 37 7 for for IN 10_1101-2021_01_07_425723 37 8 this this DT 10_1101-2021_01_07_425723 37 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 37 10 version version NN 10_1101-2021_01_07_425723 37 11 posted post VBD 10_1101-2021_01_07_425723 37 12 January January NNP 10_1101-2021_01_07_425723 37 13 8 8 CD 10_1101-2021_01_07_425723 37 14 , , , 10_1101-2021_01_07_425723 37 15 2021 2021 CD 10_1101-2021_01_07_425723 37 16 . . . 10_1101-2021_01_07_425723 37 17 ; ; : 10_1101-2021_01_07_425723 37 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 37 19 : : : 10_1101-2021_01_07_425723 37 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 37 21 preprint preprint NN 10_1101-2021_01_07_425723 37 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 37 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425723 37 24 5 5 CD 10_1101-2021_01_07_425723 37 25 was be VBD 10_1101-2021_01_07_425723 37 26 to to TO 10_1101-2021_01_07_425723 37 27 identify identify VB 10_1101-2021_01_07_425723 37 28 and and CC 10_1101-2021_01_07_425723 37 29 eliminate eliminate VB 10_1101-2021_01_07_425723 37 30 the the DT 10_1101-2021_01_07_425723 37 31 non non JJ 10_1101-2021_01_07_425723 37 32 - - JJ 10_1101-2021_01_07_425723 37 33 dissimilatory dissimilatory JJ 10_1101-2021_01_07_425723 37 34 oxygen oxygen NN 10_1101-2021_01_07_425723 37 35 requirements requirement NNS 10_1101-2021_01_07_425723 37 36 of of IN 10_1101-2021_01_07_425723 37 37 the the DT 10_1101-2021_01_07_425723 37 38 facultatively facultatively RB 10_1101-2021_01_07_425723 37 39 79 79 CD 10_1101-2021_01_07_425723 37 40 fermentative fermentative JJ 10_1101-2021_01_07_425723 37 41 , , , 10_1101-2021_01_07_425723 37 42 thermotolerant thermotolerant JJ 10_1101-2021_01_07_425723 37 43 yeast yeast NN 10_1101-2021_01_07_425723 37 44 K. K. NNP 10_1101-2021_01_07_425723 37 45 marxianus marxianus NN 10_1101-2021_01_07_425723 37 46 . . . 10_1101-2021_01_07_425723 38 1 To to IN 10_1101-2021_01_07_425723 38 2 this this DT 10_1101-2021_01_07_425723 38 3 end end NN 10_1101-2021_01_07_425723 38 4 , , , 10_1101-2021_01_07_425723 38 5 we -PRON- PRP 10_1101-2021_01_07_425723 38 6 analyzed analyze VBD 10_1101-2021_01_07_425723 38 7 and and CC 10_1101-2021_01_07_425723 38 8 compared compare VBN 10_1101-2021_01_07_425723 38 9 physiological physiological JJ 10_1101-2021_01_07_425723 38 10 80 80 CD 10_1101-2021_01_07_425723 38 11 and and CC 10_1101-2021_01_07_425723 38 12 transcriptional transcriptional JJ 10_1101-2021_01_07_425723 38 13 responses response NNS 10_1101-2021_01_07_425723 38 14 of of IN 10_1101-2021_01_07_425723 38 15 K. K. NNP 10_1101-2021_01_07_425723 38 16 marxianus marxianus NN 10_1101-2021_01_07_425723 38 17 and and CC 10_1101-2021_01_07_425723 38 18 S. S. NNP 10_1101-2021_01_07_425723 38 19 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 38 20 to to IN 10_1101-2021_01_07_425723 38 21 different different JJ 10_1101-2021_01_07_425723 38 22 oxygen- oxygen- NN 10_1101-2021_01_07_425723 38 23 and and CC 10_1101-2021_01_07_425723 38 24 anaerobic-81 anaerobic-81 NNP 10_1101-2021_01_07_425723 38 25 growth growth NN 10_1101-2021_01_07_425723 38 26 factor factor NN 10_1101-2021_01_07_425723 38 27 limitation limitation NN 10_1101-2021_01_07_425723 38 28 regimes regime NNS 10_1101-2021_01_07_425723 38 29 in in IN 10_1101-2021_01_07_425723 38 30 chemostat chemostat JJ 10_1101-2021_01_07_425723 38 31 cultures culture NNS 10_1101-2021_01_07_425723 38 32 . . . 10_1101-2021_01_07_425723 39 1 Based base VBN 10_1101-2021_01_07_425723 39 2 on on IN 10_1101-2021_01_07_425723 39 3 the the DT 10_1101-2021_01_07_425723 39 4 outcome outcome NN 10_1101-2021_01_07_425723 39 5 of of IN 10_1101-2021_01_07_425723 39 6 this this DT 10_1101-2021_01_07_425723 39 7 comparative comparative JJ 10_1101-2021_01_07_425723 39 8 82 82 CD 10_1101-2021_01_07_425723 39 9 analysis analysis NN 10_1101-2021_01_07_425723 39 10 , , , 10_1101-2021_01_07_425723 39 11 subsequent subsequent JJ 10_1101-2021_01_07_425723 39 12 experiments experiment NNS 10_1101-2021_01_07_425723 39 13 focused focus VBD 10_1101-2021_01_07_425723 39 14 on on IN 10_1101-2021_01_07_425723 39 15 characterization characterization NN 10_1101-2021_01_07_425723 39 16 and and CC 10_1101-2021_01_07_425723 39 17 engineering engineering NN 10_1101-2021_01_07_425723 39 18 of of IN 10_1101-2021_01_07_425723 39 19 sterol sterol NN 10_1101-2021_01_07_425723 39 20 metabolism metabolism NN 10_1101-2021_01_07_425723 39 21 and and CC 10_1101-2021_01_07_425723 39 22 83 83 CD 10_1101-2021_01_07_425723 39 23 yielded yielded NNP 10_1101-2021_01_07_425723 39 24 K. K. NNP 10_1101-2021_01_07_425723 39 25 marxianus marxianus NN 10_1101-2021_01_07_425723 39 26 strains strain VBZ 10_1101-2021_01_07_425723 39 27 that that WDT 10_1101-2021_01_07_425723 39 28 grew grow VBD 10_1101-2021_01_07_425723 39 29 anaerobically anaerobically RB 10_1101-2021_01_07_425723 39 30 at at IN 10_1101-2021_01_07_425723 39 31 45 45 CD 10_1101-2021_01_07_425723 39 32 ° ° NNS 10_1101-2021_01_07_425723 39 33 C C NNP 10_1101-2021_01_07_425723 39 34 . . . 10_1101-2021_01_07_425723 40 1 84 84 CD 10_1101-2021_01_07_425723 40 2 Results result NNS 10_1101-2021_01_07_425723 40 3 85 85 CD 10_1101-2021_01_07_425723 40 4 K. k. NN 10_1101-2021_01_07_425723 40 5 marxianus marxianus NN 10_1101-2021_01_07_425723 40 6 and and CC 10_1101-2021_01_07_425723 40 7 S. S. NNP 10_1101-2021_01_07_425723 40 8 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 40 9 show show VBP 10_1101-2021_01_07_425723 40 10 different different JJ 10_1101-2021_01_07_425723 40 11 physiological physiological JJ 10_1101-2021_01_07_425723 40 12 responses response NNS 10_1101-2021_01_07_425723 40 13 to to IN 10_1101-2021_01_07_425723 40 14 extreme extreme JJ 10_1101-2021_01_07_425723 40 15 oxygen oxygen NN 10_1101-2021_01_07_425723 40 16 limitation limitation NN 10_1101-2021_01_07_425723 40 17 86 86 CD 10_1101-2021_01_07_425723 40 18 To to TO 10_1101-2021_01_07_425723 40 19 investigate investigate VB 10_1101-2021_01_07_425723 40 20 oxygen oxygen NN 10_1101-2021_01_07_425723 40 21 requirements requirement NNS 10_1101-2021_01_07_425723 40 22 of of IN 10_1101-2021_01_07_425723 40 23 K. K. NNP 10_1101-2021_01_07_425723 40 24 marxianus marxianus NN 10_1101-2021_01_07_425723 40 25 , , , 10_1101-2021_01_07_425723 40 26 physiological physiological JJ 10_1101-2021_01_07_425723 40 27 responses response NNS 10_1101-2021_01_07_425723 40 28 of of IN 10_1101-2021_01_07_425723 40 29 strain strain NN 10_1101-2021_01_07_425723 40 30 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 40 31 were be VBD 10_1101-2021_01_07_425723 40 32 87 87 CD 10_1101-2021_01_07_425723 40 33 studied study VBN 10_1101-2021_01_07_425723 40 34 in in IN 10_1101-2021_01_07_425723 40 35 glucose glucose NN 10_1101-2021_01_07_425723 40 36 - - HYPH 10_1101-2021_01_07_425723 40 37 grown grow VBN 10_1101-2021_01_07_425723 40 38 chemostat chemostat JJ 10_1101-2021_01_07_425723 40 39 cultures culture NNS 10_1101-2021_01_07_425723 40 40 operated operate VBN 10_1101-2021_01_07_425723 40 41 at at IN 10_1101-2021_01_07_425723 40 42 a a DT 10_1101-2021_01_07_425723 40 43 dilution dilution NN 10_1101-2021_01_07_425723 40 44 rate rate NN 10_1101-2021_01_07_425723 40 45 of of IN 10_1101-2021_01_07_425723 40 46 0.10 0.10 CD 10_1101-2021_01_07_425723 40 47 h-1 h-1 NNS 10_1101-2021_01_07_425723 40 48 and and CC 10_1101-2021_01_07_425723 40 49 subjected subject VBN 10_1101-2021_01_07_425723 40 50 to to IN 10_1101-2021_01_07_425723 40 51 88 88 CD 10_1101-2021_01_07_425723 40 52 different different JJ 10_1101-2021_01_07_425723 40 53 oxygenation oxygenation NN 10_1101-2021_01_07_425723 40 54 and and CC 10_1101-2021_01_07_425723 40 55 AGF AGF NNP 10_1101-2021_01_07_425723 40 56 limitation limitation NN 10_1101-2021_01_07_425723 40 57 regimes regime NNS 10_1101-2021_01_07_425723 40 58 ( ( -LRB- 10_1101-2021_01_07_425723 40 59 Fig fig NN 10_1101-2021_01_07_425723 40 60 . . . 10_1101-2021_01_07_425723 41 1 1a 1a LS 10_1101-2021_01_07_425723 41 2 ) ) -RRB- 10_1101-2021_01_07_425723 41 3 . . . 10_1101-2021_01_07_425723 42 1 Physiological physiological JJ 10_1101-2021_01_07_425723 42 2 parameters parameter NNS 10_1101-2021_01_07_425723 42 3 of of IN 10_1101-2021_01_07_425723 42 4 K. K. NNP 10_1101-2021_01_07_425723 42 5 marxianus marxianus NN 10_1101-2021_01_07_425723 42 6 in in IN 10_1101-2021_01_07_425723 42 7 89 89 CD 10_1101-2021_01_07_425723 42 8 these these DT 10_1101-2021_01_07_425723 42 9 cultures culture NNS 10_1101-2021_01_07_425723 42 10 were be VBD 10_1101-2021_01_07_425723 42 11 compared compare VBN 10_1101-2021_01_07_425723 42 12 to to IN 10_1101-2021_01_07_425723 42 13 those those DT 10_1101-2021_01_07_425723 42 14 of of IN 10_1101-2021_01_07_425723 42 15 S. S. NNP 10_1101-2021_01_07_425723 42 16 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 42 17 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 42 18 - - HYPH 10_1101-2021_01_07_425723 42 19 7D 7D NNP 10_1101-2021_01_07_425723 42 20 subjected subject VBN 10_1101-2021_01_07_425723 42 21 to to IN 10_1101-2021_01_07_425723 42 22 the the DT 10_1101-2021_01_07_425723 42 23 same same JJ 10_1101-2021_01_07_425723 42 24 cultivation cultivation NN 10_1101-2021_01_07_425723 42 25 90 90 CD 10_1101-2021_01_07_425723 42 26 regimes regime NNS 10_1101-2021_01_07_425723 42 27 . . . 10_1101-2021_01_07_425723 43 1 91 91 CD 10_1101-2021_01_07_425723 43 2 In in IN 10_1101-2021_01_07_425723 43 3 glucose glucose NN 10_1101-2021_01_07_425723 43 4 - - HYPH 10_1101-2021_01_07_425723 43 5 limited limited JJ 10_1101-2021_01_07_425723 43 6 , , , 10_1101-2021_01_07_425723 43 7 aerobic aerobic JJ 10_1101-2021_01_07_425723 43 8 chemostat chemostat NN 10_1101-2021_01_07_425723 43 9 cultures culture NNS 10_1101-2021_01_07_425723 43 10 ( ( -LRB- 10_1101-2021_01_07_425723 43 11 supplied supply VBN 10_1101-2021_01_07_425723 43 12 with with IN 10_1101-2021_01_07_425723 43 13 0.5 0.5 CD 10_1101-2021_01_07_425723 43 14 L L NNP 10_1101-2021_01_07_425723 43 15 air·min-1 air·min-1 NNP 10_1101-2021_01_07_425723 43 16 , , , 10_1101-2021_01_07_425723 43 17 corresponding correspond VBG 10_1101-2021_01_07_425723 43 18 to to IN 10_1101-2021_01_07_425723 43 19 54 54 CD 10_1101-2021_01_07_425723 43 20 mmol mmol NN 10_1101-2021_01_07_425723 43 21 92 92 CD 10_1101-2021_01_07_425723 43 22 O2 o2 NN 10_1101-2021_01_07_425723 43 23 h-1 h-1 NNP 10_1101-2021_01_07_425723 43 24 ) ) -RRB- 10_1101-2021_01_07_425723 43 25 , , , 10_1101-2021_01_07_425723 43 26 the the DT 10_1101-2021_01_07_425723 43 27 Crabtree Crabtree NNP 10_1101-2021_01_07_425723 43 28 - - HYPH 10_1101-2021_01_07_425723 43 29 negative negative JJ 10_1101-2021_01_07_425723 43 30 yeast yeast NN 10_1101-2021_01_07_425723 43 31 K. K. NNP 10_1101-2021_01_07_425723 43 32 marxianus32 marxianus32 NNP 10_1101-2021_01_07_425723 43 33 and and CC 10_1101-2021_01_07_425723 43 34 the the DT 10_1101-2021_01_07_425723 43 35 Crabtree Crabtree NNP 10_1101-2021_01_07_425723 43 36 - - HYPH 10_1101-2021_01_07_425723 43 37 positive positive JJ 10_1101-2021_01_07_425723 43 38 yeast yeast NN 10_1101-2021_01_07_425723 43 39 S. S. NNP 10_1101-2021_01_07_425723 43 40 cerevisiae33 cerevisiae33 NNP 10_1101-2021_01_07_425723 43 41 both both DT 10_1101-2021_01_07_425723 43 42 93 93 CD 10_1101-2021_01_07_425723 43 43 exhibited exhibit VBD 10_1101-2021_01_07_425723 43 44 a a DT 10_1101-2021_01_07_425723 43 45 fully fully RB 10_1101-2021_01_07_425723 43 46 respiratory respiratory JJ 10_1101-2021_01_07_425723 43 47 dissimilation dissimilation NN 10_1101-2021_01_07_425723 43 48 of of IN 10_1101-2021_01_07_425723 43 49 glucose glucose NN 10_1101-2021_01_07_425723 43 50 , , , 10_1101-2021_01_07_425723 43 51 as as RB 10_1101-2021_01_07_425723 43 52 evident evident JJ 10_1101-2021_01_07_425723 43 53 from from IN 10_1101-2021_01_07_425723 43 54 absence absence NN 10_1101-2021_01_07_425723 43 55 of of IN 10_1101-2021_01_07_425723 43 56 ethanol ethanol NN 10_1101-2021_01_07_425723 43 57 production production NN 10_1101-2021_01_07_425723 43 58 and and CC 10_1101-2021_01_07_425723 43 59 94 94 CD 10_1101-2021_01_07_425723 43 60 a a DT 10_1101-2021_01_07_425723 43 61 respiratory respiratory JJ 10_1101-2021_01_07_425723 43 62 quotient quotient NN 10_1101-2021_01_07_425723 43 63 ( ( -LRB- 10_1101-2021_01_07_425723 43 64 RQ RQ NNP 10_1101-2021_01_07_425723 43 65 ) ) -RRB- 10_1101-2021_01_07_425723 43 66 close close JJ 10_1101-2021_01_07_425723 43 67 to to IN 10_1101-2021_01_07_425723 43 68 1 1 CD 10_1101-2021_01_07_425723 43 69 ( ( -LRB- 10_1101-2021_01_07_425723 43 70 Table table NN 10_1101-2021_01_07_425723 43 71 1 1 CD 10_1101-2021_01_07_425723 43 72 ) ) -RRB- 10_1101-2021_01_07_425723 43 73 . . . 10_1101-2021_01_07_425723 44 1 Apparent apparent JJ 10_1101-2021_01_07_425723 44 2 biomass biomass NN 10_1101-2021_01_07_425723 44 3 yields yield NNS 10_1101-2021_01_07_425723 44 4 on on IN 10_1101-2021_01_07_425723 44 5 glucose glucose NN 10_1101-2021_01_07_425723 44 6 of of IN 10_1101-2021_01_07_425723 44 7 both both DT 10_1101-2021_01_07_425723 44 8 yeasts yeast NNS 10_1101-2021_01_07_425723 44 9 95 95 CD 10_1101-2021_01_07_425723 44 10 exceeded exceed VBD 10_1101-2021_01_07_425723 44 11 0.5 0.5 CD 10_1101-2021_01_07_425723 44 12 g g NN 10_1101-2021_01_07_425723 44 13 biomass biomass NN 10_1101-2021_01_07_425723 44 14 ( ( -LRB- 10_1101-2021_01_07_425723 44 15 g g NNP 10_1101-2021_01_07_425723 44 16 glucose)-1 glucose)-1 CD 10_1101-2021_01_07_425723 44 17 and and CC 10_1101-2021_01_07_425723 44 18 were be VBD 10_1101-2021_01_07_425723 44 19 approximately approximately RB 10_1101-2021_01_07_425723 44 20 10 10 CD 10_1101-2021_01_07_425723 44 21 % % NN 10_1101-2021_01_07_425723 44 22 higher high JJR 10_1101-2021_01_07_425723 44 23 than than IN 10_1101-2021_01_07_425723 44 24 previously previously RB 10_1101-2021_01_07_425723 44 25 reported report VBN 10_1101-2021_01_07_425723 44 26 due due JJ 10_1101-2021_01_07_425723 44 27 96 96 CD 10_1101-2021_01_07_425723 44 28 to to TO 10_1101-2021_01_07_425723 44 29 co co NN 10_1101-2021_01_07_425723 44 30 - - NN 10_1101-2021_01_07_425723 44 31 consumption consumption NN 10_1101-2021_01_07_425723 44 32 of of IN 10_1101-2021_01_07_425723 44 33 ethanol ethanol NN 10_1101-2021_01_07_425723 44 34 , , , 10_1101-2021_01_07_425723 44 35 which which WDT 10_1101-2021_01_07_425723 44 36 was be VBD 10_1101-2021_01_07_425723 44 37 used use VBN 10_1101-2021_01_07_425723 44 38 as as IN 10_1101-2021_01_07_425723 44 39 solvent solvent NN 10_1101-2021_01_07_425723 44 40 for for IN 10_1101-2021_01_07_425723 44 41 the the DT 10_1101-2021_01_07_425723 44 42 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 44 43 growth growth NN 10_1101-2021_01_07_425723 44 44 factor factor NN 10_1101-2021_01_07_425723 44 45 ergosterol32,34 ergosterol32,34 NNP 10_1101-2021_01_07_425723 44 46 . . . 10_1101-2021_01_07_425723 45 1 97 97 CD 10_1101-2021_01_07_425723 45 2 At at IN 10_1101-2021_01_07_425723 45 3 a a DT 10_1101-2021_01_07_425723 45 4 reduced reduce VBN 10_1101-2021_01_07_425723 45 5 oxygen oxygen NN 10_1101-2021_01_07_425723 45 6 - - HYPH 10_1101-2021_01_07_425723 45 7 supply supply NN 10_1101-2021_01_07_425723 45 8 rate rate NN 10_1101-2021_01_07_425723 45 9 of of IN 10_1101-2021_01_07_425723 45 10 0.4 0.4 CD 10_1101-2021_01_07_425723 45 11 mmol mmol NN 10_1101-2021_01_07_425723 45 12 O2 O2 NNP 10_1101-2021_01_07_425723 45 13 h-1 h-1 NNP 10_1101-2021_01_07_425723 45 14 , , , 10_1101-2021_01_07_425723 45 15 both both DT 10_1101-2021_01_07_425723 45 16 yeasts yeast NNS 10_1101-2021_01_07_425723 45 17 exhibited exhibit VBD 10_1101-2021_01_07_425723 45 18 a a DT 10_1101-2021_01_07_425723 45 19 mixed mixed JJ 10_1101-2021_01_07_425723 45 20 respiro respiro NNP 10_1101-2021_01_07_425723 45 21 - - HYPH 10_1101-2021_01_07_425723 45 22 fermentative fermentative NNP 10_1101-2021_01_07_425723 45 23 98 98 CD 10_1101-2021_01_07_425723 45 24 glucose glucose NN 10_1101-2021_01_07_425723 45 25 metabolism metabolism NN 10_1101-2021_01_07_425723 45 26 . . . 10_1101-2021_01_07_425723 46 1 RQ RQ NNP 10_1101-2021_01_07_425723 46 2 values value NNS 10_1101-2021_01_07_425723 46 3 close close RB 10_1101-2021_01_07_425723 46 4 to to IN 10_1101-2021_01_07_425723 46 5 50 50 CD 10_1101-2021_01_07_425723 46 6 and and CC 10_1101-2021_01_07_425723 46 7 biomass biomass NN 10_1101-2021_01_07_425723 46 8 - - HYPH 10_1101-2021_01_07_425723 46 9 specific specific JJ 10_1101-2021_01_07_425723 46 10 ethanol ethanol NN 10_1101-2021_01_07_425723 46 11 - - HYPH 10_1101-2021_01_07_425723 46 12 production production NN 10_1101-2021_01_07_425723 46 13 rates rate NNS 10_1101-2021_01_07_425723 46 14 of of IN 10_1101-2021_01_07_425723 46 15 11.5 11.5 CD 10_1101-2021_01_07_425723 46 16 ± ± CD 10_1101-2021_01_07_425723 46 17 0.6 0.6 CD 10_1101-2021_01_07_425723 46 18 99 99 CD 10_1101-2021_01_07_425723 46 19 mmol·g·h-1 mmol·g·h-1 NNS 10_1101-2021_01_07_425723 46 20 for for IN 10_1101-2021_01_07_425723 46 21 K. K. NNP 10_1101-2021_01_07_425723 46 22 marxianus marxianu NNS 10_1101-2021_01_07_425723 46 23 and and CC 10_1101-2021_01_07_425723 46 24 7.5 7.5 CD 10_1101-2021_01_07_425723 46 25 ± ± CD 10_1101-2021_01_07_425723 46 26 0.1 0.1 CD 10_1101-2021_01_07_425723 46 27 mmol·g·h-1 mmol·g·h-1 NNS 10_1101-2021_01_07_425723 46 28 for for IN 10_1101-2021_01_07_425723 46 29 S. S. NNP 10_1101-2021_01_07_425723 46 30 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 46 31 ( ( -LRB- 10_1101-2021_01_07_425723 46 32 Table table NN 10_1101-2021_01_07_425723 46 33 1 1 CD 10_1101-2021_01_07_425723 46 34 ) ) -RRB- 10_1101-2021_01_07_425723 46 35 , , , 10_1101-2021_01_07_425723 46 36 indicated indicate VBD 10_1101-2021_01_07_425723 46 37 that that IN 10_1101-2021_01_07_425723 46 38 glucose glucose NN 10_1101-2021_01_07_425723 46 39 100 100 CD 10_1101-2021_01_07_425723 46 40 .CC .CC , 10_1101-2021_01_07_425723 46 41 - - HYPH 10_1101-2021_01_07_425723 46 42 BY by IN 10_1101-2021_01_07_425723 46 43 - - HYPH 10_1101-2021_01_07_425723 46 44 NC NC NNP 10_1101-2021_01_07_425723 46 45 - - HYPH 10_1101-2021_01_07_425723 46 46 ND ND NNP 10_1101-2021_01_07_425723 46 47 4.0 4.0 CD 10_1101-2021_01_07_425723 46 48 International International NNP 10_1101-2021_01_07_425723 46 49 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 46 50 under under IN 10_1101-2021_01_07_425723 46 51 a a DT 10_1101-2021_01_07_425723 46 52 ( ( -LRB- 10_1101-2021_01_07_425723 46 53 which which WDT 10_1101-2021_01_07_425723 46 54 was be VBD 10_1101-2021_01_07_425723 46 55 not not RB 10_1101-2021_01_07_425723 46 56 certified certify VBN 10_1101-2021_01_07_425723 46 57 by by IN 10_1101-2021_01_07_425723 46 58 peer peer NN 10_1101-2021_01_07_425723 46 59 review review NN 10_1101-2021_01_07_425723 46 60 ) ) -RRB- 10_1101-2021_01_07_425723 46 61 is be VBZ 10_1101-2021_01_07_425723 46 62 the the DT 10_1101-2021_01_07_425723 46 63 author author NN 10_1101-2021_01_07_425723 46 64 / / SYM 10_1101-2021_01_07_425723 46 65 funder funder NN 10_1101-2021_01_07_425723 46 66 , , , 10_1101-2021_01_07_425723 46 67 who who WP 10_1101-2021_01_07_425723 46 68 has have VBZ 10_1101-2021_01_07_425723 46 69 granted grant VBN 10_1101-2021_01_07_425723 46 70 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 46 71 a a DT 10_1101-2021_01_07_425723 46 72 license license NN 10_1101-2021_01_07_425723 46 73 to to TO 10_1101-2021_01_07_425723 46 74 display display VB 10_1101-2021_01_07_425723 46 75 the the DT 10_1101-2021_01_07_425723 46 76 preprint preprint NN 10_1101-2021_01_07_425723 46 77 in in IN 10_1101-2021_01_07_425723 46 78 perpetuity perpetuity NN 10_1101-2021_01_07_425723 46 79 . . . 10_1101-2021_01_07_425723 47 1 It -PRON- PRP 10_1101-2021_01_07_425723 47 2 is be VBZ 10_1101-2021_01_07_425723 47 3 made make VBN 10_1101-2021_01_07_425723 47 4 The the DT 10_1101-2021_01_07_425723 47 5 copyright copyright NN 10_1101-2021_01_07_425723 47 6 holder holder NN 10_1101-2021_01_07_425723 47 7 for for IN 10_1101-2021_01_07_425723 47 8 this this DT 10_1101-2021_01_07_425723 47 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 47 10 version version NN 10_1101-2021_01_07_425723 47 11 posted post VBD 10_1101-2021_01_07_425723 47 12 January January NNP 10_1101-2021_01_07_425723 47 13 8 8 CD 10_1101-2021_01_07_425723 47 14 , , , 10_1101-2021_01_07_425723 47 15 2021 2021 CD 10_1101-2021_01_07_425723 47 16 . . . 10_1101-2021_01_07_425723 47 17 ; ; : 10_1101-2021_01_07_425723 47 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 47 19 : : : 10_1101-2021_01_07_425723 47 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 47 21 preprint preprint NN 10_1101-2021_01_07_425723 47 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 47 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 47 24 6 6 CD 10_1101-2021_01_07_425723 47 25 dissimilation dissimilation NN 10_1101-2021_01_07_425723 47 26 in in IN 10_1101-2021_01_07_425723 47 27 these these DT 10_1101-2021_01_07_425723 47 28 cultures culture NNS 10_1101-2021_01_07_425723 47 29 was be VBD 10_1101-2021_01_07_425723 47 30 predominantly predominantly RB 10_1101-2021_01_07_425723 47 31 fermentative fermentative JJ 10_1101-2021_01_07_425723 47 32 . . . 10_1101-2021_01_07_425723 48 1 Biomass biomass NN 10_1101-2021_01_07_425723 48 2 - - HYPH 10_1101-2021_01_07_425723 48 3 specific specific JJ 10_1101-2021_01_07_425723 48 4 rates rate NNS 10_1101-2021_01_07_425723 48 5 of of IN 10_1101-2021_01_07_425723 48 6 glycerol glycerol NN 10_1101-2021_01_07_425723 48 7 101 101 CD 10_1101-2021_01_07_425723 48 8 production production NN 10_1101-2021_01_07_425723 48 9 which which WDT 10_1101-2021_01_07_425723 48 10 , , , 10_1101-2021_01_07_425723 48 11 under under IN 10_1101-2021_01_07_425723 48 12 oxygen oxygen NN 10_1101-2021_01_07_425723 48 13 - - HYPH 10_1101-2021_01_07_425723 48 14 limited limit VBN 10_1101-2021_01_07_425723 48 15 conditions condition NNS 10_1101-2021_01_07_425723 48 16 , , , 10_1101-2021_01_07_425723 48 17 enables enable NNS 10_1101-2021_01_07_425723 48 18 re re NN 10_1101-2021_01_07_425723 48 19 - - NN 10_1101-2021_01_07_425723 48 20 oxidation oxidation NN 10_1101-2021_01_07_425723 48 21 of of IN 10_1101-2021_01_07_425723 48 22 NADH NADH NNP 10_1101-2021_01_07_425723 48 23 generated generate VBN 10_1101-2021_01_07_425723 48 24 in in IN 10_1101-2021_01_07_425723 48 25 102 102 CD 10_1101-2021_01_07_425723 48 26 biosynthetic biosynthetic JJ 10_1101-2021_01_07_425723 48 27 reactions35 reactions35 NN 10_1101-2021_01_07_425723 48 28 , , , 10_1101-2021_01_07_425723 48 29 were be VBD 10_1101-2021_01_07_425723 48 30 approximately approximately RB 10_1101-2021_01_07_425723 48 31 2.5-fold 2.5-fold CD 10_1101-2021_01_07_425723 48 32 higher high JJR 10_1101-2021_01_07_425723 48 33 ( ( -LRB- 10_1101-2021_01_07_425723 48 34 p p NN 10_1101-2021_01_07_425723 48 35 = = SYM 10_1101-2021_01_07_425723 48 36 2.3·10 2.3·10 CD 10_1101-2021_01_07_425723 48 37 - - SYM 10_1101-2021_01_07_425723 48 38 4 4 CD 10_1101-2021_01_07_425723 48 39 ) ) -RRB- 10_1101-2021_01_07_425723 48 40 in in IN 10_1101-2021_01_07_425723 48 41 K. K. NNP 10_1101-2021_01_07_425723 48 42 marxianus marxianus NN 10_1101-2021_01_07_425723 48 43 than than IN 10_1101-2021_01_07_425723 48 44 in in IN 10_1101-2021_01_07_425723 48 45 S. S. NNP 10_1101-2021_01_07_425723 48 46 103 103 CD 10_1101-2021_01_07_425723 48 47 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 48 48 . . . 10_1101-2021_01_07_425723 49 1 Glycerol glycerol NN 10_1101-2021_01_07_425723 49 2 production production NN 10_1101-2021_01_07_425723 49 3 showed show VBD 10_1101-2021_01_07_425723 49 4 that that IN 10_1101-2021_01_07_425723 49 5 the the DT 10_1101-2021_01_07_425723 49 6 reduced reduced JJ 10_1101-2021_01_07_425723 49 7 oxygen oxygen NN 10_1101-2021_01_07_425723 49 8 - - HYPH 10_1101-2021_01_07_425723 49 9 supply supply NN 10_1101-2021_01_07_425723 49 10 rate rate NN 10_1101-2021_01_07_425723 49 11 constrained constrain VBN 10_1101-2021_01_07_425723 49 12 mitochondrial mitochondrial NN 10_1101-2021_01_07_425723 49 13 104 104 CD 10_1101-2021_01_07_425723 49 14 respiration respiration NN 10_1101-2021_01_07_425723 49 15 . . . 10_1101-2021_01_07_425723 50 1 However however RB 10_1101-2021_01_07_425723 50 2 , , , 10_1101-2021_01_07_425723 50 3 low low JJ 10_1101-2021_01_07_425723 50 4 residual residual JJ 10_1101-2021_01_07_425723 50 5 glucose glucose NN 10_1101-2021_01_07_425723 50 6 concentrations concentration NNS 10_1101-2021_01_07_425723 50 7 ( ( -LRB- 10_1101-2021_01_07_425723 50 8 Table table NN 10_1101-2021_01_07_425723 50 9 1 1 CD 10_1101-2021_01_07_425723 50 10 ) ) -RRB- 10_1101-2021_01_07_425723 50 11 indicated indicate VBD 10_1101-2021_01_07_425723 50 12 that that IN 10_1101-2021_01_07_425723 50 13 sufficient sufficient JJ 10_1101-2021_01_07_425723 50 14 oxygen oxygen NN 10_1101-2021_01_07_425723 50 15 was be VBD 10_1101-2021_01_07_425723 50 16 105 105 CD 10_1101-2021_01_07_425723 50 17 provided provide VBN 10_1101-2021_01_07_425723 50 18 to to TO 10_1101-2021_01_07_425723 50 19 meet meet VB 10_1101-2021_01_07_425723 50 20 most most JJS 10_1101-2021_01_07_425723 50 21 or or CC 10_1101-2021_01_07_425723 50 22 all all DT 10_1101-2021_01_07_425723 50 23 of of IN 10_1101-2021_01_07_425723 50 24 the the DT 10_1101-2021_01_07_425723 50 25 biosynthetic biosynthetic JJ 10_1101-2021_01_07_425723 50 26 oxygen oxygen NN 10_1101-2021_01_07_425723 50 27 requirements requirement NNS 10_1101-2021_01_07_425723 50 28 of of IN 10_1101-2021_01_07_425723 50 29 K. K. NNP 10_1101-2021_01_07_425723 50 30 marxianus marxianus NN 10_1101-2021_01_07_425723 50 31 . . . 10_1101-2021_01_07_425723 51 1 106 106 CD 10_1101-2021_01_07_425723 51 2 To to TO 10_1101-2021_01_07_425723 51 3 explore explore VB 10_1101-2021_01_07_425723 51 4 growth growth NN 10_1101-2021_01_07_425723 51 5 of of IN 10_1101-2021_01_07_425723 51 6 K. K. NNP 10_1101-2021_01_07_425723 51 7 marxianus marxianus NN 10_1101-2021_01_07_425723 51 8 under under IN 10_1101-2021_01_07_425723 51 9 an an DT 10_1101-2021_01_07_425723 51 10 even even RB 10_1101-2021_01_07_425723 51 11 more more RBR 10_1101-2021_01_07_425723 51 12 stringent stringent JJ 10_1101-2021_01_07_425723 51 13 oxygen oxygen NN 10_1101-2021_01_07_425723 51 14 - - HYPH 10_1101-2021_01_07_425723 51 15 limitation limitation NN 10_1101-2021_01_07_425723 51 16 , , , 10_1101-2021_01_07_425723 51 17 we -PRON- PRP 10_1101-2021_01_07_425723 51 18 exploited exploit VBD 10_1101-2021_01_07_425723 51 19 107 107 CD 10_1101-2021_01_07_425723 51 20 previously previously RB 10_1101-2021_01_07_425723 51 21 documented document VBN 10_1101-2021_01_07_425723 51 22 challenges challenge NNS 10_1101-2021_01_07_425723 51 23 in in IN 10_1101-2021_01_07_425723 51 24 achieving achieve VBG 10_1101-2021_01_07_425723 51 25 complete complete JJ 10_1101-2021_01_07_425723 51 26 anaerobiosis anaerobiosis NN 10_1101-2021_01_07_425723 51 27 in in IN 10_1101-2021_01_07_425723 51 28 laboratory laboratory NN 10_1101-2021_01_07_425723 51 29 bioreactors19,28 bioreactors19,28 NNP 10_1101-2021_01_07_425723 51 30 . . . 10_1101-2021_01_07_425723 52 1 108 108 CD 10_1101-2021_01_07_425723 52 2 Even even RB 10_1101-2021_01_07_425723 52 3 in in IN 10_1101-2021_01_07_425723 52 4 chemostats chemostat NNS 10_1101-2021_01_07_425723 52 5 sparged sparge VBN 10_1101-2021_01_07_425723 52 6 with with IN 10_1101-2021_01_07_425723 52 7 pure pure JJ 10_1101-2021_01_07_425723 52 8 nitrogen nitrogen NN 10_1101-2021_01_07_425723 52 9 , , , 10_1101-2021_01_07_425723 52 10 S. S. NNP 10_1101-2021_01_07_425723 52 11 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 52 12 grew grow VBD 10_1101-2021_01_07_425723 52 13 on on IN 10_1101-2021_01_07_425723 52 14 synthetic synthetic JJ 10_1101-2021_01_07_425723 52 15 medium medium NN 10_1101-2021_01_07_425723 52 16 lacking lack VBG 10_1101-2021_01_07_425723 52 17 Tween Tween NNP 10_1101-2021_01_07_425723 52 18 109 109 CD 10_1101-2021_01_07_425723 52 19 80 80 CD 10_1101-2021_01_07_425723 52 20 and and CC 10_1101-2021_01_07_425723 52 21 ergosterol ergosterol NN 10_1101-2021_01_07_425723 52 22 , , , 10_1101-2021_01_07_425723 52 23 albeit albeit IN 10_1101-2021_01_07_425723 52 24 at at IN 10_1101-2021_01_07_425723 52 25 an an DT 10_1101-2021_01_07_425723 52 26 increased increase VBN 10_1101-2021_01_07_425723 52 27 residual residual JJ 10_1101-2021_01_07_425723 52 28 glucose glucose NN 10_1101-2021_01_07_425723 52 29 concentration concentration NN 10_1101-2021_01_07_425723 52 30 ( ( -LRB- 10_1101-2021_01_07_425723 52 31 Fig fig NN 10_1101-2021_01_07_425723 52 32 . . . 10_1101-2021_01_07_425723 53 1 1 1 LS 10_1101-2021_01_07_425723 53 2 , , , 10_1101-2021_01_07_425723 53 3 Table table NN 10_1101-2021_01_07_425723 53 4 1 1 CD 10_1101-2021_01_07_425723 53 5 ) ) -RRB- 10_1101-2021_01_07_425723 53 6 . . . 10_1101-2021_01_07_425723 54 1 In in IN 10_1101-2021_01_07_425723 54 2 contrast contrast NN 10_1101-2021_01_07_425723 54 3 , , , 10_1101-2021_01_07_425723 54 4 K. K. NNP 10_1101-2021_01_07_425723 54 5 110 110 CD 10_1101-2021_01_07_425723 54 6 marxianus marxianus NN 10_1101-2021_01_07_425723 54 7 cultures culture NNS 10_1101-2021_01_07_425723 54 8 sparged sparge VBN 10_1101-2021_01_07_425723 54 9 with with IN 10_1101-2021_01_07_425723 54 10 pure pure JJ 10_1101-2021_01_07_425723 54 11 N2 N2 NNP 10_1101-2021_01_07_425723 54 12 and and CC 10_1101-2021_01_07_425723 54 13 supplemented supplement VBN 10_1101-2021_01_07_425723 54 14 with with IN 10_1101-2021_01_07_425723 54 15 both both DT 10_1101-2021_01_07_425723 54 16 AGFs agf NNS 10_1101-2021_01_07_425723 54 17 consumed consume VBN 10_1101-2021_01_07_425723 54 18 only only RB 10_1101-2021_01_07_425723 54 19 20 20 CD 10_1101-2021_01_07_425723 54 20 % % NN 10_1101-2021_01_07_425723 54 21 of of IN 10_1101-2021_01_07_425723 54 22 the the DT 10_1101-2021_01_07_425723 54 23 111 111 CD 10_1101-2021_01_07_425723 54 24 glucose glucose NN 10_1101-2021_01_07_425723 54 25 fed feed VBN 10_1101-2021_01_07_425723 54 26 to to IN 10_1101-2021_01_07_425723 54 27 the the DT 10_1101-2021_01_07_425723 54 28 cultures culture NNS 10_1101-2021_01_07_425723 54 29 . . . 10_1101-2021_01_07_425723 55 1 These these DT 10_1101-2021_01_07_425723 55 2 severely severely RB 10_1101-2021_01_07_425723 55 3 oxygen oxygen NN 10_1101-2021_01_07_425723 55 4 - - HYPH 10_1101-2021_01_07_425723 55 5 limited limit VBN 10_1101-2021_01_07_425723 55 6 cultures culture NNS 10_1101-2021_01_07_425723 55 7 showed show VBD 10_1101-2021_01_07_425723 55 8 a a DT 10_1101-2021_01_07_425723 55 9 residual residual JJ 10_1101-2021_01_07_425723 55 10 glucose glucose NN 10_1101-2021_01_07_425723 55 11 112 112 CD 10_1101-2021_01_07_425723 55 12 concentration concentration NN 10_1101-2021_01_07_425723 55 13 of of IN 10_1101-2021_01_07_425723 55 14 15.9 15.9 CD 10_1101-2021_01_07_425723 55 15 ± ± CD 10_1101-2021_01_07_425723 55 16 0.3 0.3 CD 10_1101-2021_01_07_425723 55 17 g·L-1 g·L-1 NNP 10_1101-2021_01_07_425723 55 18 and and CC 10_1101-2021_01_07_425723 55 19 a a DT 10_1101-2021_01_07_425723 55 20 low low JJ 10_1101-2021_01_07_425723 55 21 but but CC 10_1101-2021_01_07_425723 55 22 constant constant JJ 10_1101-2021_01_07_425723 55 23 biomass biomass NN 10_1101-2021_01_07_425723 55 24 concentration concentration NN 10_1101-2021_01_07_425723 55 25 of of IN 10_1101-2021_01_07_425723 55 26 0.4 0.4 CD 10_1101-2021_01_07_425723 55 27 ± ± CD 10_1101-2021_01_07_425723 55 28 0.0 0.0 CD 10_1101-2021_01_07_425723 55 29 g·L-1 g·l-1 NN 10_1101-2021_01_07_425723 55 30 . . . 10_1101-2021_01_07_425723 56 1 This this DT 10_1101-2021_01_07_425723 56 2 113 113 CD 10_1101-2021_01_07_425723 56 3 pronounced pronounce VBD 10_1101-2021_01_07_425723 56 4 response response NN 10_1101-2021_01_07_425723 56 5 of of IN 10_1101-2021_01_07_425723 56 6 K. K. NNP 10_1101-2021_01_07_425723 56 7 marxianus marxianus NN 10_1101-2021_01_07_425723 56 8 to to IN 10_1101-2021_01_07_425723 56 9 extreme extreme JJ 10_1101-2021_01_07_425723 56 10 oxygen oxygen NN 10_1101-2021_01_07_425723 56 11 - - HYPH 10_1101-2021_01_07_425723 56 12 limitation limitation NN 10_1101-2021_01_07_425723 56 13 provided provide VBD 10_1101-2021_01_07_425723 56 14 an an DT 10_1101-2021_01_07_425723 56 15 experimental experimental JJ 10_1101-2021_01_07_425723 56 16 context context NN 10_1101-2021_01_07_425723 56 17 114 114 CD 10_1101-2021_01_07_425723 56 18 for for IN 10_1101-2021_01_07_425723 56 19 further further RB 10_1101-2021_01_07_425723 56 20 analyzing analyze VBG 10_1101-2021_01_07_425723 56 21 its -PRON- PRP$ 10_1101-2021_01_07_425723 56 22 unknown unknown JJ 10_1101-2021_01_07_425723 56 23 oxygen oxygen NN 10_1101-2021_01_07_425723 56 24 requirements requirement NNS 10_1101-2021_01_07_425723 56 25 . . . 10_1101-2021_01_07_425723 57 1 115 115 CD 10_1101-2021_01_07_425723 57 2 S. S. NNP 10_1101-2021_01_07_425723 57 3 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 57 4 can can MD 10_1101-2021_01_07_425723 57 5 import import VB 10_1101-2021_01_07_425723 57 6 exogenous exogenous JJ 10_1101-2021_01_07_425723 57 7 sterols sterol NNS 10_1101-2021_01_07_425723 57 8 under under IN 10_1101-2021_01_07_425723 57 9 severely severely RB 10_1101-2021_01_07_425723 57 10 oxygen oxygen NN 10_1101-2021_01_07_425723 57 11 - - HYPH 10_1101-2021_01_07_425723 57 12 limited limit VBN 10_1101-2021_01_07_425723 57 13 or or CC 10_1101-2021_01_07_425723 57 14 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 57 15 conditions20 conditions20 NNP 10_1101-2021_01_07_425723 57 16 . . . 10_1101-2021_01_07_425723 58 1 If if IN 10_1101-2021_01_07_425723 58 2 116 116 CD 10_1101-2021_01_07_425723 58 3 the the DT 10_1101-2021_01_07_425723 58 4 latter latter JJ 10_1101-2021_01_07_425723 58 5 were be VBD 10_1101-2021_01_07_425723 58 6 also also RB 10_1101-2021_01_07_425723 58 7 true true JJ 10_1101-2021_01_07_425723 58 8 for for IN 10_1101-2021_01_07_425723 58 9 K. K. NNP 10_1101-2021_01_07_425723 58 10 marxianus marxianus NNP 10_1101-2021_01_07_425723 58 11 , , , 10_1101-2021_01_07_425723 58 12 omission omission NN 10_1101-2021_01_07_425723 58 13 of of IN 10_1101-2021_01_07_425723 58 14 ergosterol ergosterol NN 10_1101-2021_01_07_425723 58 15 from from IN 10_1101-2021_01_07_425723 58 16 the the DT 10_1101-2021_01_07_425723 58 17 growth growth NN 10_1101-2021_01_07_425723 58 18 medium medium NN 10_1101-2021_01_07_425723 58 19 of of IN 10_1101-2021_01_07_425723 58 20 severely severely RB 10_1101-2021_01_07_425723 58 21 117 117 CD 10_1101-2021_01_07_425723 58 22 oxygen oxygen NN 10_1101-2021_01_07_425723 58 23 - - HYPH 10_1101-2021_01_07_425723 58 24 limited limit VBN 10_1101-2021_01_07_425723 58 25 cultures culture NNS 10_1101-2021_01_07_425723 58 26 would would MD 10_1101-2021_01_07_425723 58 27 increase increase VB 10_1101-2021_01_07_425723 58 28 biomass biomass NN 10_1101-2021_01_07_425723 58 29 - - HYPH 10_1101-2021_01_07_425723 58 30 specific specific JJ 10_1101-2021_01_07_425723 58 31 oxygen oxygen NN 10_1101-2021_01_07_425723 58 32 requirements requirement NNS 10_1101-2021_01_07_425723 58 33 and and CC 10_1101-2021_01_07_425723 58 34 lead lead VBP 10_1101-2021_01_07_425723 58 35 to to IN 10_1101-2021_01_07_425723 58 36 an an DT 10_1101-2021_01_07_425723 58 37 even even RB 10_1101-2021_01_07_425723 58 38 lower low JJR 10_1101-2021_01_07_425723 58 39 118 118 CD 10_1101-2021_01_07_425723 58 40 biomass biomass NN 10_1101-2021_01_07_425723 58 41 concentration concentration NN 10_1101-2021_01_07_425723 58 42 . . . 10_1101-2021_01_07_425723 59 1 In in IN 10_1101-2021_01_07_425723 59 2 practice practice NN 10_1101-2021_01_07_425723 59 3 however however RB 10_1101-2021_01_07_425723 59 4 , , , 10_1101-2021_01_07_425723 59 5 omission omission NN 10_1101-2021_01_07_425723 59 6 of of IN 10_1101-2021_01_07_425723 59 7 ergosterol ergosterol NN 10_1101-2021_01_07_425723 59 8 led lead VBD 10_1101-2021_01_07_425723 59 9 to to IN 10_1101-2021_01_07_425723 59 10 a a DT 10_1101-2021_01_07_425723 59 11 small small JJ 10_1101-2021_01_07_425723 59 12 increase increase NN 10_1101-2021_01_07_425723 59 13 of of IN 10_1101-2021_01_07_425723 59 14 the the DT 10_1101-2021_01_07_425723 59 15 119 119 CD 10_1101-2021_01_07_425723 59 16 biomass biomass NN 10_1101-2021_01_07_425723 59 17 concentration concentration NN 10_1101-2021_01_07_425723 59 18 and and CC 10_1101-2021_01_07_425723 59 19 a a DT 10_1101-2021_01_07_425723 59 20 corresponding correspond VBG 10_1101-2021_01_07_425723 59 21 decrease decrease NN 10_1101-2021_01_07_425723 59 22 of of IN 10_1101-2021_01_07_425723 59 23 the the DT 10_1101-2021_01_07_425723 59 24 residual residual JJ 10_1101-2021_01_07_425723 59 25 glucose glucose NNP 10_1101-2021_01_07_425723 59 26 concentration concentration NN 10_1101-2021_01_07_425723 59 27 in in IN 10_1101-2021_01_07_425723 59 28 severely severely RB 10_1101-2021_01_07_425723 59 29 120 120 CD 10_1101-2021_01_07_425723 59 30 oxygen oxygen NN 10_1101-2021_01_07_425723 59 31 - - HYPH 10_1101-2021_01_07_425723 59 32 limited limit VBN 10_1101-2021_01_07_425723 59 33 chemostat chemostat JJ 10_1101-2021_01_07_425723 59 34 cultures culture NNS 10_1101-2021_01_07_425723 59 35 ( ( -LRB- 10_1101-2021_01_07_425723 59 36 Fig fig NN 10_1101-2021_01_07_425723 59 37 . . . 10_1101-2021_01_07_425723 60 1 1b 1b NNP 10_1101-2021_01_07_425723 60 2 , , , 10_1101-2021_01_07_425723 60 3 Table Table NNP 10_1101-2021_01_07_425723 60 4 1 1 CD 10_1101-2021_01_07_425723 60 5 ) ) -RRB- 10_1101-2021_01_07_425723 60 6 . . . 10_1101-2021_01_07_425723 61 1 This this DT 10_1101-2021_01_07_425723 61 2 observation observation NN 10_1101-2021_01_07_425723 61 3 suggested suggest VBD 10_1101-2021_01_07_425723 61 4 that that IN 10_1101-2021_01_07_425723 61 5 , , , 10_1101-2021_01_07_425723 61 6 in in IN 10_1101-2021_01_07_425723 61 7 contrast contrast NN 10_1101-2021_01_07_425723 61 8 to to IN 10_1101-2021_01_07_425723 61 9 S. S. NNP 10_1101-2021_01_07_425723 61 10 121 121 CD 10_1101-2021_01_07_425723 61 11 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 61 12 , , , 10_1101-2021_01_07_425723 61 13 K. K. NNP 10_1101-2021_01_07_425723 61 14 marxianus marxianus NN 10_1101-2021_01_07_425723 61 15 can can MD 10_1101-2021_01_07_425723 61 16 not not RB 10_1101-2021_01_07_425723 61 17 replace replace VB 10_1101-2021_01_07_425723 61 18 de de FW 10_1101-2021_01_07_425723 61 19 novo novo NNP 10_1101-2021_01_07_425723 61 20 oxygen oxygen NN 10_1101-2021_01_07_425723 61 21 - - HYPH 10_1101-2021_01_07_425723 61 22 dependent dependent JJ 10_1101-2021_01_07_425723 61 23 sterol sterol JJ 10_1101-2021_01_07_425723 61 24 synthesis synthesis NN 10_1101-2021_01_07_425723 61 25 by by IN 10_1101-2021_01_07_425723 61 26 uptake uptake NN 10_1101-2021_01_07_425723 61 27 of of IN 10_1101-2021_01_07_425723 61 28 122 122 CD 10_1101-2021_01_07_425723 61 29 exogenous exogenous JJ 10_1101-2021_01_07_425723 61 30 sterols sterol NNS 10_1101-2021_01_07_425723 61 31 . . . 10_1101-2021_01_07_425723 62 1 123 123 CD 10_1101-2021_01_07_425723 62 2 .CC .CC : 10_1101-2021_01_07_425723 62 3 - - HYPH 10_1101-2021_01_07_425723 62 4 BY by IN 10_1101-2021_01_07_425723 62 5 - - HYPH 10_1101-2021_01_07_425723 62 6 NC NC NNP 10_1101-2021_01_07_425723 62 7 - - HYPH 10_1101-2021_01_07_425723 62 8 ND ND NNP 10_1101-2021_01_07_425723 62 9 4.0 4.0 CD 10_1101-2021_01_07_425723 62 10 International International NNP 10_1101-2021_01_07_425723 62 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 62 12 under under IN 10_1101-2021_01_07_425723 62 13 a a DT 10_1101-2021_01_07_425723 62 14 ( ( -LRB- 10_1101-2021_01_07_425723 62 15 which which WDT 10_1101-2021_01_07_425723 62 16 was be VBD 10_1101-2021_01_07_425723 62 17 not not RB 10_1101-2021_01_07_425723 62 18 certified certify VBN 10_1101-2021_01_07_425723 62 19 by by IN 10_1101-2021_01_07_425723 62 20 peer peer NN 10_1101-2021_01_07_425723 62 21 review review NN 10_1101-2021_01_07_425723 62 22 ) ) -RRB- 10_1101-2021_01_07_425723 62 23 is be VBZ 10_1101-2021_01_07_425723 62 24 the the DT 10_1101-2021_01_07_425723 62 25 author author NN 10_1101-2021_01_07_425723 62 26 / / SYM 10_1101-2021_01_07_425723 62 27 funder funder NN 10_1101-2021_01_07_425723 62 28 , , , 10_1101-2021_01_07_425723 62 29 who who WP 10_1101-2021_01_07_425723 62 30 has have VBZ 10_1101-2021_01_07_425723 62 31 granted grant VBN 10_1101-2021_01_07_425723 62 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 62 33 a a DT 10_1101-2021_01_07_425723 62 34 license license NN 10_1101-2021_01_07_425723 62 35 to to TO 10_1101-2021_01_07_425723 62 36 display display VB 10_1101-2021_01_07_425723 62 37 the the DT 10_1101-2021_01_07_425723 62 38 preprint preprint NN 10_1101-2021_01_07_425723 62 39 in in IN 10_1101-2021_01_07_425723 62 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 62 41 . . . 10_1101-2021_01_07_425723 63 1 It -PRON- PRP 10_1101-2021_01_07_425723 63 2 is be VBZ 10_1101-2021_01_07_425723 63 3 made make VBN 10_1101-2021_01_07_425723 63 4 The the DT 10_1101-2021_01_07_425723 63 5 copyright copyright NN 10_1101-2021_01_07_425723 63 6 holder holder NN 10_1101-2021_01_07_425723 63 7 for for IN 10_1101-2021_01_07_425723 63 8 this this DT 10_1101-2021_01_07_425723 63 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 63 10 version version NN 10_1101-2021_01_07_425723 63 11 posted post VBD 10_1101-2021_01_07_425723 63 12 January January NNP 10_1101-2021_01_07_425723 63 13 8 8 CD 10_1101-2021_01_07_425723 63 14 , , , 10_1101-2021_01_07_425723 63 15 2021 2021 CD 10_1101-2021_01_07_425723 63 16 . . . 10_1101-2021_01_07_425723 63 17 ; ; : 10_1101-2021_01_07_425723 63 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 63 19 : : : 10_1101-2021_01_07_425723 63 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 63 21 preprint preprint NN 10_1101-2021_01_07_425723 63 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 63 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 63 24 7 7 CD 10_1101-2021_01_07_425723 63 25 Fig Fig NNP 10_1101-2021_01_07_425723 63 26 . . . 10_1101-2021_01_07_425723 64 1 1 1 CD 10_1101-2021_01_07_425723 64 2 | | JJ 10_1101-2021_01_07_425723 64 3 Chemostat chemostat JJ 10_1101-2021_01_07_425723 64 4 cultivation cultivation NN 10_1101-2021_01_07_425723 64 5 of of IN 10_1101-2021_01_07_425723 64 6 S. S. NNP 10_1101-2021_01_07_425723 64 7 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 64 8 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 64 9 - - HYPH 10_1101-2021_01_07_425723 64 10 7D 7D NNP 10_1101-2021_01_07_425723 64 11 and and CC 10_1101-2021_01_07_425723 64 12 K. K. NNP 10_1101-2021_01_07_425723 64 13 marxianus marxianus NN 10_1101-2021_01_07_425723 64 14 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 64 15 under under IN 10_1101-2021_01_07_425723 64 16 124 124 CD 10_1101-2021_01_07_425723 64 17 different different JJ 10_1101-2021_01_07_425723 64 18 aeration aeration NN 10_1101-2021_01_07_425723 64 19 and and CC 10_1101-2021_01_07_425723 64 20 anaerobic anaerobic NN 10_1101-2021_01_07_425723 64 21 - - HYPH 10_1101-2021_01_07_425723 64 22 growth growth NN 10_1101-2021_01_07_425723 64 23 - - HYPH 10_1101-2021_01_07_425723 64 24 factor factor NN 10_1101-2021_01_07_425723 64 25 ( ( -LRB- 10_1101-2021_01_07_425723 64 26 AGF AGF NNP 10_1101-2021_01_07_425723 64 27 ) ) -RRB- 10_1101-2021_01_07_425723 64 28 supplementation supplementation NN 10_1101-2021_01_07_425723 64 29 regimes regime NNS 10_1101-2021_01_07_425723 64 30 . . . 10_1101-2021_01_07_425723 65 1 The the DT 10_1101-2021_01_07_425723 65 2 ingoing ingoing JJ 10_1101-2021_01_07_425723 65 3 gas gas NN 10_1101-2021_01_07_425723 65 4 flow flow NN 10_1101-2021_01_07_425723 65 5 125 125 CD 10_1101-2021_01_07_425723 65 6 of of IN 10_1101-2021_01_07_425723 65 7 all all DT 10_1101-2021_01_07_425723 65 8 cultures culture NNS 10_1101-2021_01_07_425723 65 9 was be VBD 10_1101-2021_01_07_425723 65 10 500 500 CD 10_1101-2021_01_07_425723 65 11 mL·min-1 mL·min-1 NNS 10_1101-2021_01_07_425723 65 12 , , , 10_1101-2021_01_07_425723 65 13 with with IN 10_1101-2021_01_07_425723 65 14 oxygen oxygen NN 10_1101-2021_01_07_425723 65 15 partial partial JJ 10_1101-2021_01_07_425723 65 16 pressures pressure NNS 10_1101-2021_01_07_425723 65 17 of of IN 10_1101-2021_01_07_425723 65 18 21·104 21·104 CD 10_1101-2021_01_07_425723 65 19 ppm ppm NNP 10_1101-2021_01_07_425723 65 20 ( ( -LRB- 10_1101-2021_01_07_425723 65 21 O21·104 o21·104 CD 10_1101-2021_01_07_425723 65 22 ) ) -RRB- 10_1101-2021_01_07_425723 65 23 , , , 10_1101-2021_01_07_425723 65 24 840 840 CD 10_1101-2021_01_07_425723 65 25 ppm ppm NN 10_1101-2021_01_07_425723 65 26 126 126 CD 10_1101-2021_01_07_425723 65 27 ( ( -LRB- 10_1101-2021_01_07_425723 65 28 O840 O840 NNP 10_1101-2021_01_07_425723 65 29 ) ) -RRB- 10_1101-2021_01_07_425723 65 30 , , , 10_1101-2021_01_07_425723 65 31 or or CC 10_1101-2021_01_07_425723 65 32 < < XX 10_1101-2021_01_07_425723 65 33 0.5 0.5 CD 10_1101-2021_01_07_425723 65 34 ppm ppm NN 10_1101-2021_01_07_425723 65 35 ( ( -LRB- 10_1101-2021_01_07_425723 65 36 O0.5 O0.5 NNP 10_1101-2021_01_07_425723 65 37 ) ) -RRB- 10_1101-2021_01_07_425723 65 38 . . . 10_1101-2021_01_07_425723 66 1 The the DT 10_1101-2021_01_07_425723 66 2 AGFs agf NNS 10_1101-2021_01_07_425723 66 3 Ergosterol ergosterol VBP 10_1101-2021_01_07_425723 66 4 ( ( -LRB- 10_1101-2021_01_07_425723 66 5 E e NN 10_1101-2021_01_07_425723 66 6 ) ) -RRB- 10_1101-2021_01_07_425723 66 7 and/or and/or CC 10_1101-2021_01_07_425723 66 8 Tween tween NN 10_1101-2021_01_07_425723 66 9 80 80 CD 10_1101-2021_01_07_425723 66 10 ( ( -LRB- 10_1101-2021_01_07_425723 66 11 T t NN 10_1101-2021_01_07_425723 66 12 ) ) -RRB- 10_1101-2021_01_07_425723 66 13 were be VBD 10_1101-2021_01_07_425723 66 14 added add VBN 10_1101-2021_01_07_425723 66 15 to to IN 10_1101-2021_01_07_425723 66 16 media medium NNS 10_1101-2021_01_07_425723 66 17 as as IN 10_1101-2021_01_07_425723 66 18 127 127 CD 10_1101-2021_01_07_425723 66 19 indicated indicate VBD 10_1101-2021_01_07_425723 66 20 . . . 10_1101-2021_01_07_425723 67 1 a a DT 10_1101-2021_01_07_425723 67 2 , , , 10_1101-2021_01_07_425723 67 3 Schematic schematic JJ 10_1101-2021_01_07_425723 67 4 representation representation NN 10_1101-2021_01_07_425723 67 5 of of IN 10_1101-2021_01_07_425723 67 6 experimental experimental JJ 10_1101-2021_01_07_425723 67 7 set set NN 10_1101-2021_01_07_425723 67 8 - - HYPH 10_1101-2021_01_07_425723 67 9 up up RP 10_1101-2021_01_07_425723 67 10 . . . 10_1101-2021_01_07_425723 68 1 Data datum NNS 10_1101-2021_01_07_425723 68 2 for for IN 10_1101-2021_01_07_425723 68 3 each each DT 10_1101-2021_01_07_425723 68 4 cultivation cultivation NN 10_1101-2021_01_07_425723 68 5 regime regime NN 10_1101-2021_01_07_425723 68 6 were be VBD 10_1101-2021_01_07_425723 68 7 128 128 CD 10_1101-2021_01_07_425723 68 8 obtained obtain VBN 10_1101-2021_01_07_425723 68 9 from from IN 10_1101-2021_01_07_425723 68 10 independent independent JJ 10_1101-2021_01_07_425723 68 11 replicate replicate NN 10_1101-2021_01_07_425723 68 12 chemostat chemostat JJ 10_1101-2021_01_07_425723 68 13 cultures culture NNS 10_1101-2021_01_07_425723 68 14 . . . 10_1101-2021_01_07_425723 69 1 b b LS 10_1101-2021_01_07_425723 69 2 , , , 10_1101-2021_01_07_425723 69 3 Residual residual JJ 10_1101-2021_01_07_425723 69 4 glucose glucose NN 10_1101-2021_01_07_425723 69 5 concentrations concentration NNS 10_1101-2021_01_07_425723 69 6 and and CC 10_1101-2021_01_07_425723 69 7 129 129 CD 10_1101-2021_01_07_425723 69 8 .CC .CC , 10_1101-2021_01_07_425723 69 9 - - HYPH 10_1101-2021_01_07_425723 69 10 BY by IN 10_1101-2021_01_07_425723 69 11 - - HYPH 10_1101-2021_01_07_425723 69 12 NC NC NNP 10_1101-2021_01_07_425723 69 13 - - HYPH 10_1101-2021_01_07_425723 69 14 ND ND NNP 10_1101-2021_01_07_425723 69 15 4.0 4.0 CD 10_1101-2021_01_07_425723 69 16 International International NNP 10_1101-2021_01_07_425723 69 17 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 69 18 under under IN 10_1101-2021_01_07_425723 69 19 a a DT 10_1101-2021_01_07_425723 69 20 ( ( -LRB- 10_1101-2021_01_07_425723 69 21 which which WDT 10_1101-2021_01_07_425723 69 22 was be VBD 10_1101-2021_01_07_425723 69 23 not not RB 10_1101-2021_01_07_425723 69 24 certified certify VBN 10_1101-2021_01_07_425723 69 25 by by IN 10_1101-2021_01_07_425723 69 26 peer peer NN 10_1101-2021_01_07_425723 69 27 review review NN 10_1101-2021_01_07_425723 69 28 ) ) -RRB- 10_1101-2021_01_07_425723 69 29 is be VBZ 10_1101-2021_01_07_425723 69 30 the the DT 10_1101-2021_01_07_425723 69 31 author author NN 10_1101-2021_01_07_425723 69 32 / / SYM 10_1101-2021_01_07_425723 69 33 funder funder NN 10_1101-2021_01_07_425723 69 34 , , , 10_1101-2021_01_07_425723 69 35 who who WP 10_1101-2021_01_07_425723 69 36 has have VBZ 10_1101-2021_01_07_425723 69 37 granted grant VBN 10_1101-2021_01_07_425723 69 38 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 69 39 a a DT 10_1101-2021_01_07_425723 69 40 license license NN 10_1101-2021_01_07_425723 69 41 to to TO 10_1101-2021_01_07_425723 69 42 display display VB 10_1101-2021_01_07_425723 69 43 the the DT 10_1101-2021_01_07_425723 69 44 preprint preprint NN 10_1101-2021_01_07_425723 69 45 in in IN 10_1101-2021_01_07_425723 69 46 perpetuity perpetuity NN 10_1101-2021_01_07_425723 69 47 . . . 10_1101-2021_01_07_425723 70 1 It -PRON- PRP 10_1101-2021_01_07_425723 70 2 is be VBZ 10_1101-2021_01_07_425723 70 3 made make VBN 10_1101-2021_01_07_425723 70 4 The the DT 10_1101-2021_01_07_425723 70 5 copyright copyright NN 10_1101-2021_01_07_425723 70 6 holder holder NN 10_1101-2021_01_07_425723 70 7 for for IN 10_1101-2021_01_07_425723 70 8 this this DT 10_1101-2021_01_07_425723 70 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 70 10 version version NN 10_1101-2021_01_07_425723 70 11 posted post VBD 10_1101-2021_01_07_425723 70 12 January January NNP 10_1101-2021_01_07_425723 70 13 8 8 CD 10_1101-2021_01_07_425723 70 14 , , , 10_1101-2021_01_07_425723 70 15 2021 2021 CD 10_1101-2021_01_07_425723 70 16 . . . 10_1101-2021_01_07_425723 70 17 ; ; : 10_1101-2021_01_07_425723 70 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 70 19 : : : 10_1101-2021_01_07_425723 70 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 70 21 preprint preprint NN 10_1101-2021_01_07_425723 70 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 70 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 70 24 8 8 CD 10_1101-2021_01_07_425723 70 25 biomass biomass NN 10_1101-2021_01_07_425723 70 26 - - HYPH 10_1101-2021_01_07_425723 70 27 specific specific JJ 10_1101-2021_01_07_425723 70 28 oxygen oxygen NN 10_1101-2021_01_07_425723 70 29 consumption consumption NN 10_1101-2021_01_07_425723 70 30 rates rate NNS 10_1101-2021_01_07_425723 70 31 ( ( -LRB- 10_1101-2021_01_07_425723 70 32 qO2 qO2 NNP 10_1101-2021_01_07_425723 70 33 ) ) -RRB- 10_1101-2021_01_07_425723 70 34 under under IN 10_1101-2021_01_07_425723 70 35 different different JJ 10_1101-2021_01_07_425723 70 36 aeration aeration NN 10_1101-2021_01_07_425723 70 37 and and CC 10_1101-2021_01_07_425723 70 38 AGF AGF NNP 10_1101-2021_01_07_425723 70 39 - - HYPH 10_1101-2021_01_07_425723 70 40 supplementation supplementation NNP 10_1101-2021_01_07_425723 70 41 130 130 CD 10_1101-2021_01_07_425723 70 42 regimes regime NNS 10_1101-2021_01_07_425723 70 43 . . . 10_1101-2021_01_07_425723 71 1 Data datum NNS 10_1101-2021_01_07_425723 71 2 represent represent VBP 10_1101-2021_01_07_425723 71 3 mean mean JJ 10_1101-2021_01_07_425723 71 4 and and CC 10_1101-2021_01_07_425723 71 5 standard standard JJ 10_1101-2021_01_07_425723 71 6 deviation deviation NN 10_1101-2021_01_07_425723 71 7 of of IN 10_1101-2021_01_07_425723 71 8 independent independent JJ 10_1101-2021_01_07_425723 71 9 replicate replicate NN 10_1101-2021_01_07_425723 71 10 chemostat chemostat JJ 10_1101-2021_01_07_425723 71 11 cultures culture NNS 10_1101-2021_01_07_425723 71 12 . . . 10_1101-2021_01_07_425723 72 1 c c LS 10_1101-2021_01_07_425723 72 2 , , , 10_1101-2021_01_07_425723 72 3 131 131 CD 10_1101-2021_01_07_425723 72 4 Distribution distribution NN 10_1101-2021_01_07_425723 72 5 of of IN 10_1101-2021_01_07_425723 72 6 consumed consume VBN 10_1101-2021_01_07_425723 72 7 glucose glucose NN 10_1101-2021_01_07_425723 72 8 over over IN 10_1101-2021_01_07_425723 72 9 biomass biomass NN 10_1101-2021_01_07_425723 72 10 and and CC 10_1101-2021_01_07_425723 72 11 products product NNS 10_1101-2021_01_07_425723 72 12 in in IN 10_1101-2021_01_07_425723 72 13 chemostat chemostat JJ 10_1101-2021_01_07_425723 72 14 cultures culture NNS 10_1101-2021_01_07_425723 72 15 of of IN 10_1101-2021_01_07_425723 72 16 S. S. NNP 10_1101-2021_01_07_425723 72 17 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 72 18 ( ( -LRB- 10_1101-2021_01_07_425723 72 19 left left RB 10_1101-2021_01_07_425723 72 20 132 132 CD 10_1101-2021_01_07_425723 72 21 column column NN 10_1101-2021_01_07_425723 72 22 ) ) -RRB- 10_1101-2021_01_07_425723 72 23 and and CC 10_1101-2021_01_07_425723 72 24 K. K. NNP 10_1101-2021_01_07_425723 72 25 marxianus marxianus NNP 10_1101-2021_01_07_425723 72 26 ( ( -LRB- 10_1101-2021_01_07_425723 72 27 right right JJ 10_1101-2021_01_07_425723 72 28 column column NN 10_1101-2021_01_07_425723 72 29 ) ) -RRB- 10_1101-2021_01_07_425723 72 30 , , , 10_1101-2021_01_07_425723 72 31 normalized normalize VBD 10_1101-2021_01_07_425723 72 32 to to IN 10_1101-2021_01_07_425723 72 33 a a DT 10_1101-2021_01_07_425723 72 34 glucose glucose NN 10_1101-2021_01_07_425723 72 35 uptake uptake JJ 10_1101-2021_01_07_425723 72 36 rate rate NN 10_1101-2021_01_07_425723 72 37 of of IN 10_1101-2021_01_07_425723 72 38 1.00 1.00 CD 10_1101-2021_01_07_425723 72 39 mol·h-1 mol·h-1 NNS 10_1101-2021_01_07_425723 72 40 . . . 10_1101-2021_01_07_425723 73 1 Numbers number NNS 10_1101-2021_01_07_425723 73 2 133 133 CD 10_1101-2021_01_07_425723 73 3 in in IN 10_1101-2021_01_07_425723 73 4 boxes box NNS 10_1101-2021_01_07_425723 73 5 indicate indicate VBP 10_1101-2021_01_07_425723 73 6 averages average NNS 10_1101-2021_01_07_425723 73 7 of of IN 10_1101-2021_01_07_425723 73 8 measured measured JJ 10_1101-2021_01_07_425723 73 9 metabolite metabolite JJ 10_1101-2021_01_07_425723 73 10 formation formation NN 10_1101-2021_01_07_425723 73 11 rates rate NNS 10_1101-2021_01_07_425723 73 12 ( ( -LRB- 10_1101-2021_01_07_425723 73 13 mol·h-1 mol·h-1 NNP 10_1101-2021_01_07_425723 73 14 ) ) -RRB- 10_1101-2021_01_07_425723 73 15 and and CC 10_1101-2021_01_07_425723 73 16 biomass biomass NN 10_1101-2021_01_07_425723 73 17 production production NN 10_1101-2021_01_07_425723 73 18 134 134 CD 10_1101-2021_01_07_425723 73 19 rates rate NNS 10_1101-2021_01_07_425723 73 20 ( ( -LRB- 10_1101-2021_01_07_425723 73 21 g g NNP 10_1101-2021_01_07_425723 73 22 dry dry JJ 10_1101-2021_01_07_425723 73 23 weight·h-1 weight·h-1 NNP 10_1101-2021_01_07_425723 73 24 ) ) -RRB- 10_1101-2021_01_07_425723 73 25 for for IN 10_1101-2021_01_07_425723 73 26 each each DT 10_1101-2021_01_07_425723 73 27 aeration aeration NN 10_1101-2021_01_07_425723 73 28 and and CC 10_1101-2021_01_07_425723 73 29 AGF AGF NNP 10_1101-2021_01_07_425723 73 30 supplementation supplementation NN 10_1101-2021_01_07_425723 73 31 regime regime NN 10_1101-2021_01_07_425723 73 32 . . . 10_1101-2021_01_07_425723 74 1 135 135 CD 10_1101-2021_01_07_425723 74 2 Table table NN 10_1101-2021_01_07_425723 74 3 1 1 CD 10_1101-2021_01_07_425723 74 4 | | NNP 10_1101-2021_01_07_425723 74 5 Physiology Physiology NNP 10_1101-2021_01_07_425723 74 6 of of IN 10_1101-2021_01_07_425723 74 7 S. S. NNP 10_1101-2021_01_07_425723 74 8 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 74 9 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 74 10 - - HYPH 10_1101-2021_01_07_425723 74 11 7D 7D NNP 10_1101-2021_01_07_425723 74 12 and and CC 10_1101-2021_01_07_425723 74 13 K. K. NNP 10_1101-2021_01_07_425723 74 14 marxianus marxianus NN 10_1101-2021_01_07_425723 74 15 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 74 16 in in IN 10_1101-2021_01_07_425723 74 17 glucose glucose NN 10_1101-2021_01_07_425723 74 18 - - HYPH 10_1101-2021_01_07_425723 74 19 grown grow VBN 10_1101-2021_01_07_425723 74 20 136 136 CD 10_1101-2021_01_07_425723 74 21 chemostat chemostat JJ 10_1101-2021_01_07_425723 74 22 cultures culture NNS 10_1101-2021_01_07_425723 74 23 with with IN 10_1101-2021_01_07_425723 74 24 different different JJ 10_1101-2021_01_07_425723 74 25 aeration aeration NN 10_1101-2021_01_07_425723 74 26 and and CC 10_1101-2021_01_07_425723 74 27 anaerobic anaerobic NN 10_1101-2021_01_07_425723 74 28 - - HYPH 10_1101-2021_01_07_425723 74 29 growth growth NN 10_1101-2021_01_07_425723 74 30 - - HYPH 10_1101-2021_01_07_425723 74 31 factor factor NN 10_1101-2021_01_07_425723 74 32 ( ( -LRB- 10_1101-2021_01_07_425723 74 33 AGF AGF NNP 10_1101-2021_01_07_425723 74 34 ) ) -RRB- 10_1101-2021_01_07_425723 74 35 supplementation supplementation NN 10_1101-2021_01_07_425723 74 36 137 137 CD 10_1101-2021_01_07_425723 74 37 regimes regime NNS 10_1101-2021_01_07_425723 74 38 . . . 10_1101-2021_01_07_425723 75 1 Cultures culture NNS 10_1101-2021_01_07_425723 75 2 were be VBD 10_1101-2021_01_07_425723 75 3 grown grow VBN 10_1101-2021_01_07_425723 75 4 at at IN 10_1101-2021_01_07_425723 75 5 pH pH NNP 10_1101-2021_01_07_425723 75 6 6.0 6.0 CD 10_1101-2021_01_07_425723 75 7 on on IN 10_1101-2021_01_07_425723 75 8 synthetic synthetic JJ 10_1101-2021_01_07_425723 75 9 medium medium NN 10_1101-2021_01_07_425723 75 10 with with IN 10_1101-2021_01_07_425723 75 11 urea urea NNP 10_1101-2021_01_07_425723 75 12 as as IN 10_1101-2021_01_07_425723 75 13 nitrogen nitrogen NN 10_1101-2021_01_07_425723 75 14 source source NN 10_1101-2021_01_07_425723 75 15 and and CC 10_1101-2021_01_07_425723 75 16 7.5 7.5 CD 10_1101-2021_01_07_425723 75 17 g·L-1 g·L-1 NNP 10_1101-2021_01_07_425723 75 18 138 138 CD 10_1101-2021_01_07_425723 75 19 glucose glucose NN 10_1101-2021_01_07_425723 75 20 ( ( -LRB- 10_1101-2021_01_07_425723 75 21 aerobic aerobic JJ 10_1101-2021_01_07_425723 75 22 cultures culture NNS 10_1101-2021_01_07_425723 75 23 ) ) -RRB- 10_1101-2021_01_07_425723 75 24 or or CC 10_1101-2021_01_07_425723 75 25 20 20 CD 10_1101-2021_01_07_425723 75 26 g·L-1 g·l-1 CD 10_1101-2021_01_07_425723 75 27 glucose glucose NN 10_1101-2021_01_07_425723 75 28 ( ( -LRB- 10_1101-2021_01_07_425723 75 29 oxygen oxygen NN 10_1101-2021_01_07_425723 75 30 - - HYPH 10_1101-2021_01_07_425723 75 31 limited limit VBN 10_1101-2021_01_07_425723 75 32 cultures culture NNS 10_1101-2021_01_07_425723 75 33 ) ) -RRB- 10_1101-2021_01_07_425723 75 34 as as IN 10_1101-2021_01_07_425723 75 35 carbon carbon NN 10_1101-2021_01_07_425723 75 36 and and CC 10_1101-2021_01_07_425723 75 37 energy energy NN 10_1101-2021_01_07_425723 75 38 source source NN 10_1101-2021_01_07_425723 75 39 . . . 10_1101-2021_01_07_425723 76 1 139 139 CD 10_1101-2021_01_07_425723 76 2 Data datum NNS 10_1101-2021_01_07_425723 76 3 are be VBP 10_1101-2021_01_07_425723 76 4 represented represent VBN 10_1101-2021_01_07_425723 76 5 as as IN 10_1101-2021_01_07_425723 76 6 mean mean NNP 10_1101-2021_01_07_425723 76 7 ± ± NNP 10_1101-2021_01_07_425723 76 8 SE SE NNP 10_1101-2021_01_07_425723 76 9 of of IN 10_1101-2021_01_07_425723 76 10 data datum NNS 10_1101-2021_01_07_425723 76 11 from from IN 10_1101-2021_01_07_425723 76 12 independent independent JJ 10_1101-2021_01_07_425723 76 13 chemostat chemostat JJ 10_1101-2021_01_07_425723 76 14 cultures culture NNS 10_1101-2021_01_07_425723 76 15 for for IN 10_1101-2021_01_07_425723 76 16 each each DT 10_1101-2021_01_07_425723 76 17 condition condition NN 10_1101-2021_01_07_425723 76 18 . . . 10_1101-2021_01_07_425723 77 1 140 140 CD 10_1101-2021_01_07_425723 77 2 The the DT 10_1101-2021_01_07_425723 77 3 AGFs agf NNS 10_1101-2021_01_07_425723 77 4 ergosterol ergosterol VBP 10_1101-2021_01_07_425723 77 5 ( ( -LRB- 10_1101-2021_01_07_425723 77 6 E e NN 10_1101-2021_01_07_425723 77 7 ) ) -RRB- 10_1101-2021_01_07_425723 77 8 and and CC 10_1101-2021_01_07_425723 77 9 Tween tween NN 10_1101-2021_01_07_425723 77 10 80 80 CD 10_1101-2021_01_07_425723 77 11 ( ( -LRB- 10_1101-2021_01_07_425723 77 12 T t NN 10_1101-2021_01_07_425723 77 13 ) ) -RRB- 10_1101-2021_01_07_425723 77 14 were be VBD 10_1101-2021_01_07_425723 77 15 added add VBN 10_1101-2021_01_07_425723 77 16 to to IN 10_1101-2021_01_07_425723 77 17 the the DT 10_1101-2021_01_07_425723 77 18 media medium NNS 10_1101-2021_01_07_425723 77 19 as as IN 10_1101-2021_01_07_425723 77 20 indicated indicate VBN 10_1101-2021_01_07_425723 77 21 . . . 10_1101-2021_01_07_425723 78 1 Cultures culture NNS 10_1101-2021_01_07_425723 78 2 were be VBD 10_1101-2021_01_07_425723 78 3 aerated aerate VBN 10_1101-2021_01_07_425723 78 4 141 141 CD 10_1101-2021_01_07_425723 78 5 at at IN 10_1101-2021_01_07_425723 78 6 500 500 CD 10_1101-2021_01_07_425723 78 7 mL·min-1 mL·min-1 NNS 10_1101-2021_01_07_425723 78 8 with with IN 10_1101-2021_01_07_425723 78 9 gas gas NN 10_1101-2021_01_07_425723 78 10 mixtures mixture NNS 10_1101-2021_01_07_425723 78 11 containing contain VBG 10_1101-2021_01_07_425723 78 12 21·104 21·104 CD 10_1101-2021_01_07_425723 78 13 ppm ppm NNP 10_1101-2021_01_07_425723 78 14 O2 O2 NNP 10_1101-2021_01_07_425723 78 15 ( ( -LRB- 10_1101-2021_01_07_425723 78 16 O21·104 O21·104 NNP 10_1101-2021_01_07_425723 78 17 ) ) -RRB- 10_1101-2021_01_07_425723 78 18 , , , 10_1101-2021_01_07_425723 78 19 840 840 CD 10_1101-2021_01_07_425723 78 20 ppm ppm NN 10_1101-2021_01_07_425723 78 21 O2 O2 NNP 10_1101-2021_01_07_425723 78 22 ( ( -LRB- 10_1101-2021_01_07_425723 78 23 O840 o840 NN 10_1101-2021_01_07_425723 78 24 ) ) -RRB- 10_1101-2021_01_07_425723 78 25 or or CC 10_1101-2021_01_07_425723 78 26 < < XX 10_1101-2021_01_07_425723 78 27 0.5 0.5 CD 10_1101-2021_01_07_425723 78 28 ppm ppm NN 10_1101-2021_01_07_425723 78 29 142 142 CD 10_1101-2021_01_07_425723 78 30 O2 o2 NN 10_1101-2021_01_07_425723 78 31 ( ( -LRB- 10_1101-2021_01_07_425723 78 32 O0.5 O0.5 NNP 10_1101-2021_01_07_425723 78 33 ) ) -RRB- 10_1101-2021_01_07_425723 78 34 . . . 10_1101-2021_01_07_425723 79 1 Tween tween NN 10_1101-2021_01_07_425723 79 2 80 80 CD 10_1101-2021_01_07_425723 79 3 was be VBD 10_1101-2021_01_07_425723 79 4 omitted omit VBN 10_1101-2021_01_07_425723 79 5 from from IN 10_1101-2021_01_07_425723 79 6 media medium NNS 10_1101-2021_01_07_425723 79 7 used use VBN 10_1101-2021_01_07_425723 79 8 for for IN 10_1101-2021_01_07_425723 79 9 aerobic aerobic JJ 10_1101-2021_01_07_425723 79 10 cultivation cultivation NN 10_1101-2021_01_07_425723 79 11 to to TO 10_1101-2021_01_07_425723 79 12 prevent prevent VB 10_1101-2021_01_07_425723 79 13 excessive excessive JJ 10_1101-2021_01_07_425723 79 14 foaming foaming NN 10_1101-2021_01_07_425723 79 15 . . . 10_1101-2021_01_07_425723 80 1 143 143 CD 10_1101-2021_01_07_425723 80 2 Ethanol ethanol NN 10_1101-2021_01_07_425723 80 3 measurements measurement NNS 10_1101-2021_01_07_425723 80 4 were be VBD 10_1101-2021_01_07_425723 80 5 corrected correct VBN 10_1101-2021_01_07_425723 80 6 for for IN 10_1101-2021_01_07_425723 80 7 evaporation evaporation NN 10_1101-2021_01_07_425723 80 8 ( ( -LRB- 10_1101-2021_01_07_425723 80 9 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 80 10 Fig Fig NNP 10_1101-2021_01_07_425723 80 11 . . . 10_1101-2021_01_07_425723 81 1 1 1 LS 10_1101-2021_01_07_425723 81 2 ) ) -RRB- 10_1101-2021_01_07_425723 81 3 . . . 10_1101-2021_01_07_425723 82 1 Positive positive JJ 10_1101-2021_01_07_425723 82 2 and and CC 10_1101-2021_01_07_425723 82 3 negative negative JJ 10_1101-2021_01_07_425723 82 4 144 144 CD 10_1101-2021_01_07_425723 82 5 biomass biomass NN 10_1101-2021_01_07_425723 82 6 - - HYPH 10_1101-2021_01_07_425723 82 7 specific specific JJ 10_1101-2021_01_07_425723 82 8 conversion conversion NN 10_1101-2021_01_07_425723 82 9 rates rate NNS 10_1101-2021_01_07_425723 82 10 ( ( -LRB- 10_1101-2021_01_07_425723 82 11 q q NNP 10_1101-2021_01_07_425723 82 12 ) ) -RRB- 10_1101-2021_01_07_425723 82 13 represent represent VB 10_1101-2021_01_07_425723 82 14 consumption consumption NN 10_1101-2021_01_07_425723 82 15 and and CC 10_1101-2021_01_07_425723 82 16 production production NN 10_1101-2021_01_07_425723 82 17 rates rate NNS 10_1101-2021_01_07_425723 82 18 , , , 10_1101-2021_01_07_425723 82 19 respectively respectively RB 10_1101-2021_01_07_425723 82 20 . . . 10_1101-2021_01_07_425723 83 1 145 145 CD 10_1101-2021_01_07_425723 83 2 S. S. NNP 10_1101-2021_01_07_425723 83 3 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 83 4 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 83 5 - - HYPH 10_1101-2021_01_07_425723 83 6 7D 7D NNP 10_1101-2021_01_07_425723 83 7 K. K. NNP 10_1101-2021_01_07_425723 83 8 marxianus marxianus NN 10_1101-2021_01_07_425723 83 9 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 83 10 Condition Condition NNP 10_1101-2021_01_07_425723 83 11 1 1 CD 10_1101-2021_01_07_425723 83 12 2 2 CD 10_1101-2021_01_07_425723 83 13 3 3 CD 10_1101-2021_01_07_425723 83 14 4 4 CD 10_1101-2021_01_07_425723 83 15 5 5 CD 10_1101-2021_01_07_425723 83 16 1 1 CD 10_1101-2021_01_07_425723 83 17 2 2 CD 10_1101-2021_01_07_425723 83 18 3 3 CD 10_1101-2021_01_07_425723 83 19 4 4 CD 10_1101-2021_01_07_425723 83 20 Aeration Aeration NNP 10_1101-2021_01_07_425723 83 21 regime regime NN 10_1101-2021_01_07_425723 83 22 O21·1 o21·1 $ 10_1101-2021_01_07_425723 83 23 04 04 CD 10_1101-2021_01_07_425723 83 24 O840 O840 NNP 10_1101-2021_01_07_425723 83 25 O0.5 O0.5 NNP 10_1101-2021_01_07_425723 83 26 O0.5 O0.5 NNP 10_1101-2021_01_07_425723 83 27 O0.5 O0.5 NNP 10_1101-2021_01_07_425723 83 28 O21·1 O21·1 NNP 10_1101-2021_01_07_425723 83 29 04 04 CD 10_1101-2021_01_07_425723 83 30 O840 O840 NNP 10_1101-2021_01_07_425723 83 31 O0.5 O0.5 NNP 10_1101-2021_01_07_425723 83 32 O0.5 O0.5 NNP 10_1101-2021_01_07_425723 83 33 AGF AGF NNP 10_1101-2021_01_07_425723 83 34 E E NNP 10_1101-2021_01_07_425723 83 35 TE TE NNP 10_1101-2021_01_07_425723 83 36 TE TE NNP 10_1101-2021_01_07_425723 83 37 T T NNP 10_1101-2021_01_07_425723 83 38 - - HYPH 10_1101-2021_01_07_425723 83 39 E E NNP 10_1101-2021_01_07_425723 83 40 TE TE NNP 10_1101-2021_01_07_425723 83 41 TE TE NNP 10_1101-2021_01_07_425723 83 42 T T NNP 10_1101-2021_01_07_425723 83 43 Replicates replicate NNS 10_1101-2021_01_07_425723 83 44 3 3 CD 10_1101-2021_01_07_425723 83 45 3 3 CD 10_1101-2021_01_07_425723 83 46 2 2 CD 10_1101-2021_01_07_425723 83 47 5 5 CD 10_1101-2021_01_07_425723 83 48 2 2 CD 10_1101-2021_01_07_425723 83 49 2 2 CD 10_1101-2021_01_07_425723 83 50 5 5 CD 10_1101-2021_01_07_425723 83 51 2 2 CD 10_1101-2021_01_07_425723 83 52 2 2 CD 10_1101-2021_01_07_425723 83 53 D d NN 10_1101-2021_01_07_425723 83 54 ( ( -LRB- 10_1101-2021_01_07_425723 83 55 h-1 h-1 NNP 10_1101-2021_01_07_425723 83 56 ) ) -RRB- 10_1101-2021_01_07_425723 83 57 0.10 0.10 CD 10_1101-2021_01_07_425723 83 58 ± ± CD 10_1101-2021_01_07_425723 83 59 0.00 0.00 CD 10_1101-2021_01_07_425723 83 60 0.10 0.10 CD 10_1101-2021_01_07_425723 83 61 ± ± CD 10_1101-2021_01_07_425723 83 62 0.00 0.00 CD 10_1101-2021_01_07_425723 83 63 0.10 0.10 CD 10_1101-2021_01_07_425723 83 64 ± ± CD 10_1101-2021_01_07_425723 83 65 0.00 0.00 CD 10_1101-2021_01_07_425723 83 66 0.10 0.10 CD 10_1101-2021_01_07_425723 83 67 ± ± CD 10_1101-2021_01_07_425723 83 68 0.00 0.00 CD 10_1101-2021_01_07_425723 83 69 0.10 0.10 CD 10_1101-2021_01_07_425723 83 70 ± ± CD 10_1101-2021_01_07_425723 83 71 0.00 0.00 CD 10_1101-2021_01_07_425723 83 72 0.10 0.10 CD 10_1101-2021_01_07_425723 83 73 ± ± CD 10_1101-2021_01_07_425723 83 74 0.00 0.00 CD 10_1101-2021_01_07_425723 83 75 0.11 0.11 CD 10_1101-2021_01_07_425723 83 76 ± ± CD 10_1101-2021_01_07_425723 83 77 0.01 0.01 CD 10_1101-2021_01_07_425723 83 78 0.12 0.12 CD 10_1101-2021_01_07_425723 83 79 ± ± CD 10_1101-2021_01_07_425723 83 80 0.01 0.01 CD 10_1101-2021_01_07_425723 83 81 0.12 0.12 CD 10_1101-2021_01_07_425723 83 82 ± ± CD 10_1101-2021_01_07_425723 83 83 0.01 0.01 CD 10_1101-2021_01_07_425723 83 84 Biomass Biomass NNP 10_1101-2021_01_07_425723 83 85 ( ( -LRB- 10_1101-2021_01_07_425723 83 86 g·L-1 g·L-1 NNP 10_1101-2021_01_07_425723 83 87 ) ) -RRB- 10_1101-2021_01_07_425723 83 88 4.22 4.22 CD 10_1101-2021_01_07_425723 83 89 ± ± CD 10_1101-2021_01_07_425723 83 90 0.06 0.06 CD 10_1101-2021_01_07_425723 83 91 2.29 2.29 CD 10_1101-2021_01_07_425723 83 92 ± ± CD 10_1101-2021_01_07_425723 83 93 0.04 0.04 CD 10_1101-2021_01_07_425723 83 94 1.98 1.98 CD 10_1101-2021_01_07_425723 83 95 ± ± CD 10_1101-2021_01_07_425723 83 96 0.01 0.01 CD 10_1101-2021_01_07_425723 83 97 1.56 1.56 CD 10_1101-2021_01_07_425723 83 98 ± ± CD 10_1101-2021_01_07_425723 83 99 0.03 0.03 CD 10_1101-2021_01_07_425723 83 100 1.12 1.12 CD 10_1101-2021_01_07_425723 83 101 ± ± CD 10_1101-2021_01_07_425723 83 102 0.02 0.02 CD 10_1101-2021_01_07_425723 83 103 3.79 3.79 CD 10_1101-2021_01_07_425723 83 104 ± ± CD 10_1101-2021_01_07_425723 83 105 0.02 0.02 CD 10_1101-2021_01_07_425723 83 106 1.57 1.57 CD 10_1101-2021_01_07_425723 83 107 ± ± CD 10_1101-2021_01_07_425723 83 108 0.10 0.10 CD 10_1101-2021_01_07_425723 83 109 0.35 0.35 CD 10_1101-2021_01_07_425723 83 110 ± ± CD 10_1101-2021_01_07_425723 83 111 0.02 0.02 CD 10_1101-2021_01_07_425723 83 112 0.50 0.50 CD 10_1101-2021_01_07_425723 83 113 ± ± CD 10_1101-2021_01_07_425723 83 114 0.04 0.04 CD 10_1101-2021_01_07_425723 83 115 Residual residual JJ 10_1101-2021_01_07_425723 83 116 glucose glucose NN 10_1101-2021_01_07_425723 83 117 ( ( -LRB- 10_1101-2021_01_07_425723 83 118 g·L-1 g·l-1 UH 10_1101-2021_01_07_425723 83 119 ) ) -RRB- 10_1101-2021_01_07_425723 83 120 0.00 0.00 CD 10_1101-2021_01_07_425723 83 121 ± ± CD 10_1101-2021_01_07_425723 83 122 0.00 0.00 CD 10_1101-2021_01_07_425723 83 123 0.07 0.07 CD 10_1101-2021_01_07_425723 83 124 ± ± CD 10_1101-2021_01_07_425723 83 125 0.00 0.00 CD 10_1101-2021_01_07_425723 83 126 0.06 0.06 CD 10_1101-2021_01_07_425723 83 127 ± ± CD 10_1101-2021_01_07_425723 83 128 0.02 0.02 CD 10_1101-2021_01_07_425723 83 129 0.23 0.23 CD 10_1101-2021_01_07_425723 83 130 ± ± CD 10_1101-2021_01_07_425723 83 131 0.04 0.04 CD 10_1101-2021_01_07_425723 83 132 1.47 1.47 CD 10_1101-2021_01_07_425723 83 133 ± ± CD 10_1101-2021_01_07_425723 83 134 0.01 0.01 CD 10_1101-2021_01_07_425723 83 135 0.00 0.00 CD 10_1101-2021_01_07_425723 83 136 ± ± CD 10_1101-2021_01_07_425723 83 137 0.00 0.00 CD 10_1101-2021_01_07_425723 83 138 0.10 0.10 CD 10_1101-2021_01_07_425723 83 139 ± ± CD 10_1101-2021_01_07_425723 83 140 0.02 0.02 CD 10_1101-2021_01_07_425723 83 141 15.92 15.92 CD 10_1101-2021_01_07_425723 83 142 ± ± CD 10_1101-2021_01_07_425723 83 143 0.26 0.26 CD 10_1101-2021_01_07_425723 83 144 13.67 13.67 CD 10_1101-2021_01_07_425723 83 145 ± ± CD 10_1101-2021_01_07_425723 83 146 0.16 0.16 CD 10_1101-2021_01_07_425723 83 147 Y Y NNP 10_1101-2021_01_07_425723 83 148 biomass biomass NN 10_1101-2021_01_07_425723 83 149 / / SYM 10_1101-2021_01_07_425723 83 150 glucose glucose NN 10_1101-2021_01_07_425723 83 151 ( ( -LRB- 10_1101-2021_01_07_425723 83 152 g·g-1 g·g-1 NNP 10_1101-2021_01_07_425723 83 153 ) ) -RRB- 10_1101-2021_01_07_425723 83 154 0.57 0.57 CD 10_1101-2021_01_07_425723 83 155 ± ± CD 10_1101-2021_01_07_425723 83 156 0.01 0.01 CD 10_1101-2021_01_07_425723 83 157 0.12 0.12 CD 10_1101-2021_01_07_425723 83 158 ± ± CD 10_1101-2021_01_07_425723 83 159 0.00 0.00 CD 10_1101-2021_01_07_425723 83 160 0.10 0.10 CD 10_1101-2021_01_07_425723 83 161 ± ± CD 10_1101-2021_01_07_425723 83 162 0.00 0.00 CD 10_1101-2021_01_07_425723 83 163 0.08 0.08 CD 10_1101-2021_01_07_425723 83 164 ± ± CD 10_1101-2021_01_07_425723 83 165 0.00 0.00 CD 10_1101-2021_01_07_425723 83 166 0.06 0.06 CD 10_1101-2021_01_07_425723 83 167 ± ± CD 10_1101-2021_01_07_425723 83 168 0.00 0.00 CD 10_1101-2021_01_07_425723 83 169 0.53 0.53 CD 10_1101-2021_01_07_425723 83 170 ± ± CD 10_1101-2021_01_07_425723 83 171 0.00 0.00 CD 10_1101-2021_01_07_425723 83 172 0.08 0.08 CD 10_1101-2021_01_07_425723 83 173 ± ± CD 10_1101-2021_01_07_425723 83 174 0.00 0.00 CD 10_1101-2021_01_07_425723 83 175 0.09 0.09 CD 10_1101-2021_01_07_425723 83 176 ± ± CD 10_1101-2021_01_07_425723 83 177 0.00 0.00 CD 10_1101-2021_01_07_425723 83 178 0.09 0.09 CD 10_1101-2021_01_07_425723 83 179 ± ± CD 10_1101-2021_01_07_425723 83 180 0.01 0.01 CD 10_1101-2021_01_07_425723 83 181 .CC .CC : 10_1101-2021_01_07_425723 83 182 - - HYPH 10_1101-2021_01_07_425723 83 183 BY by IN 10_1101-2021_01_07_425723 83 184 - - HYPH 10_1101-2021_01_07_425723 83 185 NC NC NNP 10_1101-2021_01_07_425723 83 186 - - HYPH 10_1101-2021_01_07_425723 83 187 ND ND NNP 10_1101-2021_01_07_425723 83 188 4.0 4.0 CD 10_1101-2021_01_07_425723 83 189 International International NNP 10_1101-2021_01_07_425723 83 190 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 83 191 under under IN 10_1101-2021_01_07_425723 83 192 a a DT 10_1101-2021_01_07_425723 83 193 ( ( -LRB- 10_1101-2021_01_07_425723 83 194 which which WDT 10_1101-2021_01_07_425723 83 195 was be VBD 10_1101-2021_01_07_425723 83 196 not not RB 10_1101-2021_01_07_425723 83 197 certified certify VBN 10_1101-2021_01_07_425723 83 198 by by IN 10_1101-2021_01_07_425723 83 199 peer peer NN 10_1101-2021_01_07_425723 83 200 review review NN 10_1101-2021_01_07_425723 83 201 ) ) -RRB- 10_1101-2021_01_07_425723 83 202 is be VBZ 10_1101-2021_01_07_425723 83 203 the the DT 10_1101-2021_01_07_425723 83 204 author author NN 10_1101-2021_01_07_425723 83 205 / / SYM 10_1101-2021_01_07_425723 83 206 funder funder NN 10_1101-2021_01_07_425723 83 207 , , , 10_1101-2021_01_07_425723 83 208 who who WP 10_1101-2021_01_07_425723 83 209 has have VBZ 10_1101-2021_01_07_425723 83 210 granted grant VBN 10_1101-2021_01_07_425723 83 211 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 83 212 a a DT 10_1101-2021_01_07_425723 83 213 license license NN 10_1101-2021_01_07_425723 83 214 to to TO 10_1101-2021_01_07_425723 83 215 display display VB 10_1101-2021_01_07_425723 83 216 the the DT 10_1101-2021_01_07_425723 83 217 preprint preprint NN 10_1101-2021_01_07_425723 83 218 in in IN 10_1101-2021_01_07_425723 83 219 perpetuity perpetuity NN 10_1101-2021_01_07_425723 83 220 . . . 10_1101-2021_01_07_425723 84 1 It -PRON- PRP 10_1101-2021_01_07_425723 84 2 is be VBZ 10_1101-2021_01_07_425723 84 3 made make VBN 10_1101-2021_01_07_425723 84 4 The the DT 10_1101-2021_01_07_425723 84 5 copyright copyright NN 10_1101-2021_01_07_425723 84 6 holder holder NN 10_1101-2021_01_07_425723 84 7 for for IN 10_1101-2021_01_07_425723 84 8 this this DT 10_1101-2021_01_07_425723 84 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 84 10 version version NN 10_1101-2021_01_07_425723 84 11 posted post VBD 10_1101-2021_01_07_425723 84 12 January January NNP 10_1101-2021_01_07_425723 84 13 8 8 CD 10_1101-2021_01_07_425723 84 14 , , , 10_1101-2021_01_07_425723 84 15 2021 2021 CD 10_1101-2021_01_07_425723 84 16 . . . 10_1101-2021_01_07_425723 84 17 ; ; : 10_1101-2021_01_07_425723 84 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 84 19 : : : 10_1101-2021_01_07_425723 84 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 84 21 preprint preprint NN 10_1101-2021_01_07_425723 84 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 84 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 84 24 9 9 CD 10_1101-2021_01_07_425723 84 25 Y Y NNP 10_1101-2021_01_07_425723 84 26 ethanol ethanol NN 10_1101-2021_01_07_425723 84 27 / / SYM 10_1101-2021_01_07_425723 84 28 glucose glucose NN 10_1101-2021_01_07_425723 84 29 ( ( -LRB- 10_1101-2021_01_07_425723 84 30 g·g-1 g·g-1 NNP 10_1101-2021_01_07_425723 84 31 ) ) -RRB- 10_1101-2021_01_07_425723 84 32 - - : 10_1101-2021_01_07_425723 84 33 1.67 1.67 CD 10_1101-2021_01_07_425723 84 34 ± ± CD 10_1101-2021_01_07_425723 84 35 0.06 0.06 CD 10_1101-2021_01_07_425723 84 36 1.63 1.63 CD 10_1101-2021_01_07_425723 84 37 ± ± CD 10_1101-2021_01_07_425723 84 38 0.02 0.02 CD 10_1101-2021_01_07_425723 84 39 1.65 1.65 CD 10_1101-2021_01_07_425723 84 40 ± ± CD 10_1101-2021_01_07_425723 84 41 0.02 0.02 CD 10_1101-2021_01_07_425723 84 42 1.68 1.68 CD 10_1101-2021_01_07_425723 84 43 ± ± CD 10_1101-2021_01_07_425723 84 44 0.02 0.02 CD 10_1101-2021_01_07_425723 84 45 - - HYPH 10_1101-2021_01_07_425723 84 46 1.53 1.53 CD 10_1101-2021_01_07_425723 84 47 ± ± CD 10_1101-2021_01_07_425723 84 48 0.03 0.03 CD 10_1101-2021_01_07_425723 84 49 1.31 1.31 CD 10_1101-2021_01_07_425723 84 50 ± ± CD 10_1101-2021_01_07_425723 84 51 0.05 0.05 CD 10_1101-2021_01_07_425723 84 52 1.40 1.40 CD 10_1101-2021_01_07_425723 84 53 ± ± CD 10_1101-2021_01_07_425723 84 54 0.02 0.02 CD 10_1101-2021_01_07_425723 84 55 qglucose qglucose NN 10_1101-2021_01_07_425723 84 56 ( ( -LRB- 10_1101-2021_01_07_425723 84 57 mmol·g·h-1 mmol·g·h-1 NNP 10_1101-2021_01_07_425723 84 58 ) ) -RRB- 10_1101-2021_01_07_425723 84 59 -0.95 -0.95 : 10_1101-2021_01_07_425723 84 60 ± ± CD 10_1101-2021_01_07_425723 84 61 0.03 0.03 CD 10_1101-2021_01_07_425723 84 62 -4.59 -4.59 CD 10_1101-2021_01_07_425723 84 63 ± ± CD 10_1101-2021_01_07_425723 84 64 0.10 0.10 CD 10_1101-2021_01_07_425723 84 65 -5.25 -5.25 CD 10_1101-2021_01_07_425723 84 66 ± ± CD 10_1101-2021_01_07_425723 84 67 0.04 0.04 CD 10_1101-2021_01_07_425723 84 68 -6.77 -6.77 CD 10_1101-2021_01_07_425723 84 69 ± ± CD 10_1101-2021_01_07_425723 84 70 0.27 0.27 CD 10_1101-2021_01_07_425723 84 71 -9.06 -9.06 CD 10_1101-2021_01_07_425723 84 72 ± ± CD 10_1101-2021_01_07_425723 84 73 0.15 0.15 CD 10_1101-2021_01_07_425723 84 74 -1.05 -1.05 CD 10_1101-2021_01_07_425723 84 75 ± ± CD 10_1101-2021_01_07_425723 84 76 0.00 0.00 CD 10_1101-2021_01_07_425723 84 77 -7.46 -7.46 CD 10_1101-2021_01_07_425723 84 78 ± ± CD 10_1101-2021_01_07_425723 84 79 0.30 0.30 CD 10_1101-2021_01_07_425723 84 80 -7.30 -7.30 CD 10_1101-2021_01_07_425723 84 81 ± ± CD 10_1101-2021_01_07_425723 84 82 0.81 0.81 CD 10_1101-2021_01_07_425723 84 83 -8.53 -8.53 CD 10_1101-2021_01_07_425723 84 84 ± ± CD 10_1101-2021_01_07_425723 84 85 0.00 0.00 CD 10_1101-2021_01_07_425723 84 86 qethanol qethanol NN 10_1101-2021_01_07_425723 84 87 ( ( -LRB- 10_1101-2021_01_07_425723 84 88 mmol·g·h-1 mmol·g·h-1 NNP 10_1101-2021_01_07_425723 84 89 ) ) -RRB- 10_1101-2021_01_07_425723 84 90 -0.44 -0.44 CD 10_1101-2021_01_07_425723 84 91 ± ± CD 10_1101-2021_01_07_425723 84 92 0.03 0.03 CD 10_1101-2021_01_07_425723 84 93 7.48 7.48 CD 10_1101-2021_01_07_425723 84 94 ± ± CD 10_1101-2021_01_07_425723 84 95 0.10 0.10 CD 10_1101-2021_01_07_425723 84 96 8.40 8.40 CD 10_1101-2021_01_07_425723 84 97 ± ± CD 10_1101-2021_01_07_425723 84 98 0.02 0.02 CD 10_1101-2021_01_07_425723 84 99 10.96 10.96 CD 10_1101-2021_01_07_425723 84 100 ± ± CD 10_1101-2021_01_07_425723 84 101 0.56 0.56 CD 10_1101-2021_01_07_425723 84 102 15.03 15.03 CD 10_1101-2021_01_07_425723 84 103 ± ± CD 10_1101-2021_01_07_425723 84 104 0.47 0.47 CD 10_1101-2021_01_07_425723 84 105 -0.52 -0.52 CD 10_1101-2021_01_07_425723 84 106 ± ± CD 10_1101-2021_01_07_425723 84 107 0.00 0.00 CD 10_1101-2021_01_07_425723 84 108 11.49 11.49 CD 10_1101-2021_01_07_425723 84 109 ± ± CD 10_1101-2021_01_07_425723 84 110 0.44 0.44 CD 10_1101-2021_01_07_425723 84 111 10.25 10.25 CD 10_1101-2021_01_07_425723 84 112 ± ± CD 10_1101-2021_01_07_425723 84 113 0.66 0.66 CD 10_1101-2021_01_07_425723 84 114 12.69 12.69 CD 10_1101-2021_01_07_425723 84 115 ± ± CD 10_1101-2021_01_07_425723 84 116 0.11 0.11 CD 10_1101-2021_01_07_425723 84 117 RQ RQ NNP 10_1101-2021_01_07_425723 84 118 1.08 1.08 CD 10_1101-2021_01_07_425723 84 119 ± ± CD 10_1101-2021_01_07_425723 84 120 0.02 0.02 CD 10_1101-2021_01_07_425723 84 121 52.2 52.2 CD 10_1101-2021_01_07_425723 84 122 ± ± CD 10_1101-2021_01_07_425723 84 123 2.4 2.4 CD 10_1101-2021_01_07_425723 84 124 - - HYPH 10_1101-2021_01_07_425723 84 125 - - : 10_1101-2021_01_07_425723 84 126 - - HYPH 10_1101-2021_01_07_425723 84 127 1.06 1.06 CD 10_1101-2021_01_07_425723 84 128 ± ± CD 10_1101-2021_01_07_425723 84 129 0.01 0.01 CD 10_1101-2021_01_07_425723 84 130 49.3 49.3 CD 10_1101-2021_01_07_425723 84 131 ± ± CD 10_1101-2021_01_07_425723 84 132 7.5 7.5 CD 10_1101-2021_01_07_425723 84 133 - - HYPH 10_1101-2021_01_07_425723 84 134 - - : 10_1101-2021_01_07_425723 84 135 Glycerol Glycerol NNP 10_1101-2021_01_07_425723 84 136 / / SYM 10_1101-2021_01_07_425723 84 137 biomass biomass NN 10_1101-2021_01_07_425723 84 138 ( ( -LRB- 10_1101-2021_01_07_425723 84 139 mmol·(g mmol·(g NNP 10_1101-2021_01_07_425723 84 140 biomass)-1 biomass)-1 NN 10_1101-2021_01_07_425723 84 141 ) ) -RRB- 10_1101-2021_01_07_425723 84 142 0.00 0.00 CD 10_1101-2021_01_07_425723 84 143 ± ± CD 10_1101-2021_01_07_425723 84 144 0.00 0.00 CD 10_1101-2021_01_07_425723 84 145 3.67 3.67 CD 10_1101-2021_01_07_425723 84 146 ± ± CD 10_1101-2021_01_07_425723 84 147 0.05 0.05 CD 10_1101-2021_01_07_425723 84 148 5.58 5.58 CD 10_1101-2021_01_07_425723 84 149 ± ± CD 10_1101-2021_01_07_425723 84 150 0.02 0.02 CD 10_1101-2021_01_07_425723 84 151 6.73 6.73 CD 10_1101-2021_01_07_425723 84 152 ± ± CD 10_1101-2021_01_07_425723 84 153 0.25 0.25 CD 10_1101-2021_01_07_425723 84 154 11.26 11.26 CD 10_1101-2021_01_07_425723 84 155 ± ± CD 10_1101-2021_01_07_425723 84 156 0.40 0.40 CD 10_1101-2021_01_07_425723 84 157 0.00 0.00 CD 10_1101-2021_01_07_425723 84 158 ± ± CD 10_1101-2021_01_07_425723 84 159 0.00 0.00 CD 10_1101-2021_01_07_425723 84 160 9.51 9.51 CD 10_1101-2021_01_07_425723 84 161 ± ± CD 10_1101-2021_01_07_425723 84 162 0.46 0.46 CD 10_1101-2021_01_07_425723 84 163 16.90 16.90 CD 10_1101-2021_01_07_425723 84 164 ± ± CD 10_1101-2021_01_07_425723 84 165 0.76 0.76 CD 10_1101-2021_01_07_425723 84 166 18.45 18.45 CD 10_1101-2021_01_07_425723 84 167 ± ± CD 10_1101-2021_01_07_425723 84 168 2.09 2.09 CD 10_1101-2021_01_07_425723 84 169 Carbon carbon NN 10_1101-2021_01_07_425723 84 170 recovery recovery NN 10_1101-2021_01_07_425723 84 171 ( ( -LRB- 10_1101-2021_01_07_425723 84 172 % % NN 10_1101-2021_01_07_425723 84 173 ) ) -RRB- 10_1101-2021_01_07_425723 84 174 99.9 99.9 CD 10_1101-2021_01_07_425723 84 175 ± ± CD 10_1101-2021_01_07_425723 84 176 0.7 0.7 CD 10_1101-2021_01_07_425723 84 177 101.2 101.2 CD 10_1101-2021_01_07_425723 84 178 ± ± CD 10_1101-2021_01_07_425723 84 179 3.3 3.3 CD 10_1101-2021_01_07_425723 84 180 100.4 100.4 CD 10_1101-2021_01_07_425723 84 181 ± ± CD 10_1101-2021_01_07_425723 84 182 0.1 0.1 CD 10_1101-2021_01_07_425723 84 183 100.1 100.1 CD 10_1101-2021_01_07_425723 84 184 ± ± CD 10_1101-2021_01_07_425723 84 185 1.3 1.3 CD 10_1101-2021_01_07_425723 84 186 104.0 104.0 CD 10_1101-2021_01_07_425723 84 187 ± ± CD 10_1101-2021_01_07_425723 84 188 0.2 0.2 CD 10_1101-2021_01_07_425723 84 189 100.5 100.5 CD 10_1101-2021_01_07_425723 84 190 ± ± CD 10_1101-2021_01_07_425723 84 191 0.1 0.1 CD 10_1101-2021_01_07_425723 84 192 91.1 91.1 CD 10_1101-2021_01_07_425723 84 193 ± ± CD 10_1101-2021_01_07_425723 84 194 2.0 2.0 CD 10_1101-2021_01_07_425723 84 195 101.6 101.6 CD 10_1101-2021_01_07_425723 84 196 ± ± CD 10_1101-2021_01_07_425723 84 197 6.5 6.5 CD 10_1101-2021_01_07_425723 84 198 99.7 99.7 CD 10_1101-2021_01_07_425723 84 199 ± ± CD 10_1101-2021_01_07_425723 84 200 3.9 3.9 CD 10_1101-2021_01_07_425723 84 201 Degree Degree NNP 10_1101-2021_01_07_425723 84 202 of of IN 10_1101-2021_01_07_425723 84 203 reduction reduction NN 10_1101-2021_01_07_425723 84 204 recovery recovery NN 10_1101-2021_01_07_425723 84 205 ( ( -LRB- 10_1101-2021_01_07_425723 84 206 % % NN 10_1101-2021_01_07_425723 84 207 ) ) -RRB- 10_1101-2021_01_07_425723 84 208 98.4 98.4 CD 10_1101-2021_01_07_425723 84 209 ± ± CD 10_1101-2021_01_07_425723 84 210 0.7 0.7 CD 10_1101-2021_01_07_425723 84 211 100.9 100.9 CD 10_1101-2021_01_07_425723 84 212 ± ± CD 10_1101-2021_01_07_425723 84 213 0.8 0.8 CD 10_1101-2021_01_07_425723 84 214 100.1 100.1 CD 10_1101-2021_01_07_425723 84 215 ± ± CD 10_1101-2021_01_07_425723 84 216 0.9 0.9 CD 10_1101-2021_01_07_425723 84 217 98.1 98.1 CD 10_1101-2021_01_07_425723 84 218 ± ± CD 10_1101-2021_01_07_425723 84 219 0.6 0.6 CD 10_1101-2021_01_07_425723 84 220 100.1 100.1 CD 10_1101-2021_01_07_425723 84 221 ± ± CD 10_1101-2021_01_07_425723 84 222 1.8 1.8 CD 10_1101-2021_01_07_425723 84 223 98.8 98.8 CD 10_1101-2021_01_07_425723 84 224 ± ± CD 10_1101-2021_01_07_425723 84 225 0.1 0.1 CD 10_1101-2021_01_07_425723 84 226 94.5 94.5 CD 10_1101-2021_01_07_425723 84 227 ± ± CD 10_1101-2021_01_07_425723 84 228 0.4 0.4 CD 10_1101-2021_01_07_425723 84 229 97.8 97.8 CD 10_1101-2021_01_07_425723 84 230 ± ± CD 10_1101-2021_01_07_425723 84 231 6.2 6.2 CD 10_1101-2021_01_07_425723 84 232 99.1 99.1 CD 10_1101-2021_01_07_425723 84 233 ± ± CD 10_1101-2021_01_07_425723 84 234 3.5 3.5 CD 10_1101-2021_01_07_425723 84 235 146 146 CD 10_1101-2021_01_07_425723 84 236 Transcriptional transcriptional JJ 10_1101-2021_01_07_425723 84 237 responses response NNS 10_1101-2021_01_07_425723 84 238 of of IN 10_1101-2021_01_07_425723 84 239 K. K. NNP 10_1101-2021_01_07_425723 84 240 marxianus marxianus NN 10_1101-2021_01_07_425723 84 241 to to IN 10_1101-2021_01_07_425723 84 242 oxygen oxygen NN 10_1101-2021_01_07_425723 84 243 limitation limitation NN 10_1101-2021_01_07_425723 84 244 involve involve VBP 10_1101-2021_01_07_425723 84 245 ergosterol ergosterol JJ 10_1101-2021_01_07_425723 84 246 metabolism metabolism NN 10_1101-2021_01_07_425723 84 247 147 147 CD 10_1101-2021_01_07_425723 84 248 To to TO 10_1101-2021_01_07_425723 84 249 further further RB 10_1101-2021_01_07_425723 84 250 investigate investigate VB 10_1101-2021_01_07_425723 84 251 the the DT 10_1101-2021_01_07_425723 84 252 non non JJ 10_1101-2021_01_07_425723 84 253 - - JJ 10_1101-2021_01_07_425723 84 254 dissimilatory dissimilatory JJ 10_1101-2021_01_07_425723 84 255 oxygen oxygen NN 10_1101-2021_01_07_425723 84 256 requirements requirement NNS 10_1101-2021_01_07_425723 84 257 of of IN 10_1101-2021_01_07_425723 84 258 K. K. NNP 10_1101-2021_01_07_425723 84 259 marxianus marxianus NN 10_1101-2021_01_07_425723 84 260 , , , 10_1101-2021_01_07_425723 84 261 transcriptome transcriptome DT 10_1101-2021_01_07_425723 84 262 148 148 CD 10_1101-2021_01_07_425723 84 263 analyses analysis NNS 10_1101-2021_01_07_425723 84 264 were be VBD 10_1101-2021_01_07_425723 84 265 performed perform VBN 10_1101-2021_01_07_425723 84 266 on on IN 10_1101-2021_01_07_425723 84 267 cultures culture NNS 10_1101-2021_01_07_425723 84 268 of of IN 10_1101-2021_01_07_425723 84 269 S. S. NNP 10_1101-2021_01_07_425723 84 270 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 84 271 and and CC 10_1101-2021_01_07_425723 84 272 K. K. NNP 10_1101-2021_01_07_425723 84 273 marxianus marxianu NNS 10_1101-2021_01_07_425723 84 274 grown grow VBN 10_1101-2021_01_07_425723 84 275 under under IN 10_1101-2021_01_07_425723 84 276 the the DT 10_1101-2021_01_07_425723 84 277 aeration aeration NN 10_1101-2021_01_07_425723 84 278 and and CC 10_1101-2021_01_07_425723 84 279 149 149 CD 10_1101-2021_01_07_425723 84 280 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 84 281 - - HYPH 10_1101-2021_01_07_425723 84 282 growth growth NN 10_1101-2021_01_07_425723 84 283 - - HYPH 10_1101-2021_01_07_425723 84 284 factor factor NN 10_1101-2021_01_07_425723 84 285 supplementation supplementation NN 10_1101-2021_01_07_425723 84 286 regimes regime NNS 10_1101-2021_01_07_425723 84 287 discussed discuss VBN 10_1101-2021_01_07_425723 84 288 above above RB 10_1101-2021_01_07_425723 84 289 . . . 10_1101-2021_01_07_425723 85 1 The the DT 10_1101-2021_01_07_425723 85 2 genome genome JJ 10_1101-2021_01_07_425723 85 3 sequence sequence NN 10_1101-2021_01_07_425723 85 4 of of IN 10_1101-2021_01_07_425723 85 5 K. K. NNP 10_1101-2021_01_07_425723 85 6 150 150 CD 10_1101-2021_01_07_425723 85 7 marxianus marxianus NN 10_1101-2021_01_07_425723 85 8 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 85 9 was be VBD 10_1101-2021_01_07_425723 85 10 only only RB 10_1101-2021_01_07_425723 85 11 available available JJ 10_1101-2021_01_07_425723 85 12 as as IN 10_1101-2021_01_07_425723 85 13 draft draft NN 10_1101-2021_01_07_425723 85 14 assembly assembly NN 10_1101-2021_01_07_425723 85 15 and and CC 10_1101-2021_01_07_425723 85 16 was be VBD 10_1101-2021_01_07_425723 85 17 not not RB 10_1101-2021_01_07_425723 85 18 annotated36 annotated36 VBN 10_1101-2021_01_07_425723 85 19 . . . 10_1101-2021_01_07_425723 86 1 Therefore therefore RB 10_1101-2021_01_07_425723 86 2 , , , 10_1101-2021_01_07_425723 86 3 long long RB 10_1101-2021_01_07_425723 86 4 - - HYPH 10_1101-2021_01_07_425723 86 5 read read VBN 10_1101-2021_01_07_425723 86 6 151 151 CD 10_1101-2021_01_07_425723 86 7 genome genome JJ 10_1101-2021_01_07_425723 86 8 sequencing sequencing NN 10_1101-2021_01_07_425723 86 9 , , , 10_1101-2021_01_07_425723 86 10 assembly assembly NN 10_1101-2021_01_07_425723 86 11 and and CC 10_1101-2021_01_07_425723 86 12 de de NNP 10_1101-2021_01_07_425723 86 13 novo novo NNP 10_1101-2021_01_07_425723 86 14 genome genome NNP 10_1101-2021_01_07_425723 86 15 annotation annotation NN 10_1101-2021_01_07_425723 86 16 were be VBD 10_1101-2021_01_07_425723 86 17 performed perform VBN 10_1101-2021_01_07_425723 86 18 , , , 10_1101-2021_01_07_425723 86 19 the the DT 10_1101-2021_01_07_425723 86 20 annotation annotation NN 10_1101-2021_01_07_425723 86 21 was be VBD 10_1101-2021_01_07_425723 86 22 152 152 CD 10_1101-2021_01_07_425723 86 23 refined refine VBN 10_1101-2021_01_07_425723 86 24 by by IN 10_1101-2021_01_07_425723 86 25 using use VBG 10_1101-2021_01_07_425723 86 26 transcriptome transcriptome DT 10_1101-2021_01_07_425723 86 27 assemblies assembly NNS 10_1101-2021_01_07_425723 86 28 ( ( -LRB- 10_1101-2021_01_07_425723 86 29 Data datum NNS 10_1101-2021_01_07_425723 86 30 availability availability NN 10_1101-2021_01_07_425723 86 31 ) ) -RRB- 10_1101-2021_01_07_425723 86 32 . . . 10_1101-2021_01_07_425723 87 1 Comparative comparative JJ 10_1101-2021_01_07_425723 87 2 transcriptome transcriptome DT 10_1101-2021_01_07_425723 87 3 analysis analysis NN 10_1101-2021_01_07_425723 87 4 of of IN 10_1101-2021_01_07_425723 87 5 S. S. NNP 10_1101-2021_01_07_425723 87 6 153 153 CD 10_1101-2021_01_07_425723 87 7 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 87 8 and and CC 10_1101-2021_01_07_425723 87 9 K. K. NNP 10_1101-2021_01_07_425723 87 10 marxianus marxianus NN 10_1101-2021_01_07_425723 87 11 focused focus VBD 10_1101-2021_01_07_425723 87 12 on on IN 10_1101-2021_01_07_425723 87 13 orthologous orthologous JJ 10_1101-2021_01_07_425723 87 14 genes gene NNS 10_1101-2021_01_07_425723 87 15 with with IN 10_1101-2021_01_07_425723 87 16 divergent divergent JJ 10_1101-2021_01_07_425723 87 17 expression expression NN 10_1101-2021_01_07_425723 87 18 patterns pattern NNS 10_1101-2021_01_07_425723 87 19 that that IN 10_1101-2021_01_07_425723 87 20 154 154 CD 10_1101-2021_01_07_425723 87 21 revealed reveal VBD 10_1101-2021_01_07_425723 87 22 a a DT 10_1101-2021_01_07_425723 87 23 strikingly strikingly RB 10_1101-2021_01_07_425723 87 24 different different JJ 10_1101-2021_01_07_425723 87 25 transcriptional transcriptional JJ 10_1101-2021_01_07_425723 87 26 response response NN 10_1101-2021_01_07_425723 87 27 to to IN 10_1101-2021_01_07_425723 87 28 growth growth NN 10_1101-2021_01_07_425723 87 29 limitation limitation NN 10_1101-2021_01_07_425723 87 30 by by IN 10_1101-2021_01_07_425723 87 31 oxygen oxygen NN 10_1101-2021_01_07_425723 87 32 and/or and/or CC 10_1101-2021_01_07_425723 87 33 anaerobic-155 anaerobic-155 NNP 10_1101-2021_01_07_425723 87 34 growth growth NN 10_1101-2021_01_07_425723 87 35 - - HYPH 10_1101-2021_01_07_425723 87 36 factor factor NN 10_1101-2021_01_07_425723 87 37 availability availability NN 10_1101-2021_01_07_425723 87 38 ( ( -LRB- 10_1101-2021_01_07_425723 87 39 Fig fig NN 10_1101-2021_01_07_425723 87 40 . . . 10_1101-2021_01_07_425723 88 1 2 2 LS 10_1101-2021_01_07_425723 88 2 ) ) -RRB- 10_1101-2021_01_07_425723 88 3 . . . 10_1101-2021_01_07_425723 89 1 156 156 CD 10_1101-2021_01_07_425723 89 2 In in IN 10_1101-2021_01_07_425723 89 3 S. S. NNP 10_1101-2021_01_07_425723 89 4 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 89 5 , , , 10_1101-2021_01_07_425723 89 6 import import NN 10_1101-2021_01_07_425723 89 7 of of IN 10_1101-2021_01_07_425723 89 8 exogenous exogenous JJ 10_1101-2021_01_07_425723 89 9 sterols sterol NNS 10_1101-2021_01_07_425723 89 10 by by IN 10_1101-2021_01_07_425723 89 11 Aus1 Aus1 NNP 10_1101-2021_01_07_425723 89 12 and and CC 10_1101-2021_01_07_425723 89 13 Pdr11 Pdr11 NNP 10_1101-2021_01_07_425723 89 14 can can MD 10_1101-2021_01_07_425723 89 15 alleviate alleviate VB 10_1101-2021_01_07_425723 89 16 the the DT 10_1101-2021_01_07_425723 89 17 impact impact NN 10_1101-2021_01_07_425723 89 18 of of IN 10_1101-2021_01_07_425723 89 19 oxygen oxygen NN 10_1101-2021_01_07_425723 89 20 157 157 CD 10_1101-2021_01_07_425723 89 21 limitation limitation NN 10_1101-2021_01_07_425723 89 22 on on IN 10_1101-2021_01_07_425723 89 23 sterol sterol JJ 10_1101-2021_01_07_425723 89 24 biosynthesis20 biosynthesis20 NNP 10_1101-2021_01_07_425723 89 25 . . . 10_1101-2021_01_07_425723 90 1 Consistent consistent JJ 10_1101-2021_01_07_425723 90 2 with with IN 10_1101-2021_01_07_425723 90 3 this this DT 10_1101-2021_01_07_425723 90 4 role role NN 10_1101-2021_01_07_425723 90 5 of of IN 10_1101-2021_01_07_425723 90 6 sterol sterol NN 10_1101-2021_01_07_425723 90 7 uptake uptake JJ 10_1101-2021_01_07_425723 90 8 , , , 10_1101-2021_01_07_425723 90 9 sterol sterol JJ 10_1101-2021_01_07_425723 90 10 biosynthetic biosynthetic JJ 10_1101-2021_01_07_425723 90 11 genes gene NNS 10_1101-2021_01_07_425723 90 12 in in IN 10_1101-2021_01_07_425723 90 13 158 158 CD 10_1101-2021_01_07_425723 90 14 S. S. NNP 10_1101-2021_01_07_425723 90 15 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 90 16 were be VBD 10_1101-2021_01_07_425723 90 17 only only RB 10_1101-2021_01_07_425723 90 18 highly highly RB 10_1101-2021_01_07_425723 90 19 upregulated upregulated JJ 10_1101-2021_01_07_425723 90 20 in in IN 10_1101-2021_01_07_425723 90 21 severely severely RB 10_1101-2021_01_07_425723 90 22 oxygen oxygen NN 10_1101-2021_01_07_425723 90 23 - - HYPH 10_1101-2021_01_07_425723 90 24 limited limit VBN 10_1101-2021_01_07_425723 90 25 cultures culture NNS 10_1101-2021_01_07_425723 90 26 when when WRB 10_1101-2021_01_07_425723 90 27 ergosterol ergosterol NNP 10_1101-2021_01_07_425723 90 28 was be VBD 10_1101-2021_01_07_425723 90 29 159 159 CD 10_1101-2021_01_07_425723 90 30 omitted omit VBN 10_1101-2021_01_07_425723 90 31 from from IN 10_1101-2021_01_07_425723 90 32 the the DT 10_1101-2021_01_07_425723 90 33 growth growth NN 10_1101-2021_01_07_425723 90 34 medium medium NN 10_1101-2021_01_07_425723 90 35 ( ( -LRB- 10_1101-2021_01_07_425723 90 36 Fig fig NN 10_1101-2021_01_07_425723 90 37 . . . 10_1101-2021_01_07_425723 91 1 3b 3b LS 10_1101-2021_01_07_425723 91 2 , , , 10_1101-2021_01_07_425723 91 3 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 91 4 Fig Fig NNP 10_1101-2021_01_07_425723 91 5 . . . 10_1101-2021_01_07_425723 92 1 6 6 CD 10_1101-2021_01_07_425723 92 2 , , , 10_1101-2021_01_07_425723 92 3 contrast contrast NN 10_1101-2021_01_07_425723 92 4 43 43 CD 10_1101-2021_01_07_425723 92 5 ) ) -RRB- 10_1101-2021_01_07_425723 92 6 . . . 10_1101-2021_01_07_425723 93 1 Also also RB 10_1101-2021_01_07_425723 93 2 the the DT 10_1101-2021_01_07_425723 93 3 mevalonate mevalonate NN 10_1101-2021_01_07_425723 93 4 160 160 CD 10_1101-2021_01_07_425723 93 5 pathway pathway NN 10_1101-2021_01_07_425723 93 6 for for IN 10_1101-2021_01_07_425723 93 7 synthesis synthesis NN 10_1101-2021_01_07_425723 93 8 of of IN 10_1101-2021_01_07_425723 93 9 the the DT 10_1101-2021_01_07_425723 93 10 sterol sterol JJ 10_1101-2021_01_07_425723 93 11 precursor precursor NN 10_1101-2021_01_07_425723 93 12 squalene squalene NN 10_1101-2021_01_07_425723 93 13 , , , 10_1101-2021_01_07_425723 93 14 which which WDT 10_1101-2021_01_07_425723 93 15 does do VBZ 10_1101-2021_01_07_425723 93 16 not not RB 10_1101-2021_01_07_425723 93 17 require require VB 10_1101-2021_01_07_425723 93 18 oxygen oxygen NN 10_1101-2021_01_07_425723 93 19 , , , 10_1101-2021_01_07_425723 93 20 was be VBD 10_1101-2021_01_07_425723 93 21 upregulated upregulated JJ 10_1101-2021_01_07_425723 93 22 161 161 CD 10_1101-2021_01_07_425723 93 23 .CC .CC NFP 10_1101-2021_01_07_425723 93 24 - - HYPH 10_1101-2021_01_07_425723 93 25 BY by IN 10_1101-2021_01_07_425723 93 26 - - HYPH 10_1101-2021_01_07_425723 93 27 NC NC NNP 10_1101-2021_01_07_425723 93 28 - - HYPH 10_1101-2021_01_07_425723 93 29 ND ND NNP 10_1101-2021_01_07_425723 93 30 4.0 4.0 CD 10_1101-2021_01_07_425723 93 31 International International NNP 10_1101-2021_01_07_425723 93 32 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 93 33 under under IN 10_1101-2021_01_07_425723 93 34 a a DT 10_1101-2021_01_07_425723 93 35 ( ( -LRB- 10_1101-2021_01_07_425723 93 36 which which WDT 10_1101-2021_01_07_425723 93 37 was be VBD 10_1101-2021_01_07_425723 93 38 not not RB 10_1101-2021_01_07_425723 93 39 certified certify VBN 10_1101-2021_01_07_425723 93 40 by by IN 10_1101-2021_01_07_425723 93 41 peer peer NN 10_1101-2021_01_07_425723 93 42 review review NN 10_1101-2021_01_07_425723 93 43 ) ) -RRB- 10_1101-2021_01_07_425723 93 44 is be VBZ 10_1101-2021_01_07_425723 93 45 the the DT 10_1101-2021_01_07_425723 93 46 author author NN 10_1101-2021_01_07_425723 93 47 / / SYM 10_1101-2021_01_07_425723 93 48 funder funder NN 10_1101-2021_01_07_425723 93 49 , , , 10_1101-2021_01_07_425723 93 50 who who WP 10_1101-2021_01_07_425723 93 51 has have VBZ 10_1101-2021_01_07_425723 93 52 granted grant VBN 10_1101-2021_01_07_425723 93 53 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 93 54 a a DT 10_1101-2021_01_07_425723 93 55 license license NN 10_1101-2021_01_07_425723 93 56 to to TO 10_1101-2021_01_07_425723 93 57 display display VB 10_1101-2021_01_07_425723 93 58 the the DT 10_1101-2021_01_07_425723 93 59 preprint preprint NN 10_1101-2021_01_07_425723 93 60 in in IN 10_1101-2021_01_07_425723 93 61 perpetuity perpetuity NN 10_1101-2021_01_07_425723 93 62 . . . 10_1101-2021_01_07_425723 94 1 It -PRON- PRP 10_1101-2021_01_07_425723 94 2 is be VBZ 10_1101-2021_01_07_425723 94 3 made make VBN 10_1101-2021_01_07_425723 94 4 The the DT 10_1101-2021_01_07_425723 94 5 copyright copyright NN 10_1101-2021_01_07_425723 94 6 holder holder NN 10_1101-2021_01_07_425723 94 7 for for IN 10_1101-2021_01_07_425723 94 8 this this DT 10_1101-2021_01_07_425723 94 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 94 10 version version NN 10_1101-2021_01_07_425723 94 11 posted post VBD 10_1101-2021_01_07_425723 94 12 January January NNP 10_1101-2021_01_07_425723 94 13 8 8 CD 10_1101-2021_01_07_425723 94 14 , , , 10_1101-2021_01_07_425723 94 15 2021 2021 CD 10_1101-2021_01_07_425723 94 16 . . . 10_1101-2021_01_07_425723 94 17 ; ; : 10_1101-2021_01_07_425723 94 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 94 19 : : : 10_1101-2021_01_07_425723 94 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 94 21 preprint preprint NN 10_1101-2021_01_07_425723 94 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 94 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425723 94 24 10 10 CD 10_1101-2021_01_07_425723 94 25 ( ( -LRB- 10_1101-2021_01_07_425723 94 26 contrast contrast NN 10_1101-2021_01_07_425723 94 27 43 43 CD 10_1101-2021_01_07_425723 94 28 ) ) -RRB- 10_1101-2021_01_07_425723 94 29 , , , 10_1101-2021_01_07_425723 94 30 reflecting reflect VBG 10_1101-2021_01_07_425723 94 31 a a DT 10_1101-2021_01_07_425723 94 32 relief relief NN 10_1101-2021_01_07_425723 94 33 of of IN 10_1101-2021_01_07_425723 94 34 feedback feedback NN 10_1101-2021_01_07_425723 94 35 regulation regulation NN 10_1101-2021_01_07_425723 94 36 by by IN 10_1101-2021_01_07_425723 94 37 ergosterol37 ergosterol37 NNP 10_1101-2021_01_07_425723 94 38 . . . 10_1101-2021_01_07_425723 95 1 In in IN 10_1101-2021_01_07_425723 95 2 contrast contrast NN 10_1101-2021_01_07_425723 95 3 , , , 10_1101-2021_01_07_425723 95 4 K. K. NNP 10_1101-2021_01_07_425723 95 5 marxianus marxianus NN 10_1101-2021_01_07_425723 95 6 showed show VBD 10_1101-2021_01_07_425723 95 7 162 162 CD 10_1101-2021_01_07_425723 95 8 a a DT 10_1101-2021_01_07_425723 95 9 pronounced pronounced JJ 10_1101-2021_01_07_425723 95 10 upregulation upregulation NN 10_1101-2021_01_07_425723 95 11 of of IN 10_1101-2021_01_07_425723 95 12 genes gene NNS 10_1101-2021_01_07_425723 95 13 involved involve VBN 10_1101-2021_01_07_425723 95 14 in in IN 10_1101-2021_01_07_425723 95 15 sterol sterol NN 10_1101-2021_01_07_425723 95 16 , , , 10_1101-2021_01_07_425723 95 17 isoprenoid isoprenoid JJ 10_1101-2021_01_07_425723 95 18 and and CC 10_1101-2021_01_07_425723 95 19 fatty fatty NN 10_1101-2021_01_07_425723 95 20 - - HYPH 10_1101-2021_01_07_425723 95 21 acid acid NN 10_1101-2021_01_07_425723 95 22 metabolism metabolism NN 10_1101-2021_01_07_425723 95 23 ( ( -LRB- 10_1101-2021_01_07_425723 95 24 Fig fig NN 10_1101-2021_01_07_425723 95 25 . . . 10_1101-2021_01_07_425723 96 1 2ab 2ab NNP 10_1101-2021_01_07_425723 96 2 , , , 10_1101-2021_01_07_425723 96 3 163 163 CD 10_1101-2021_01_07_425723 96 4 Fig Fig NNP 10_1101-2021_01_07_425723 96 5 . . . 10_1101-2021_01_07_425723 97 1 3 3 LS 10_1101-2021_01_07_425723 97 2 , , , 10_1101-2021_01_07_425723 97 3 contrast contrast NN 10_1101-2021_01_07_425723 97 4 31 31 CD 10_1101-2021_01_07_425723 97 5 ) ) -RRB- 10_1101-2021_01_07_425723 97 6 in in IN 10_1101-2021_01_07_425723 97 7 severely severely RB 10_1101-2021_01_07_425723 97 8 oxygen oxygen NN 10_1101-2021_01_07_425723 97 9 - - HYPH 10_1101-2021_01_07_425723 97 10 limited limit VBN 10_1101-2021_01_07_425723 97 11 cultures culture NNS 10_1101-2021_01_07_425723 97 12 supplemented supplement VBN 10_1101-2021_01_07_425723 97 13 with with IN 10_1101-2021_01_07_425723 97 14 ergosterol ergosterol NN 10_1101-2021_01_07_425723 97 15 and and CC 10_1101-2021_01_07_425723 97 16 Tween Tween NNP 10_1101-2021_01_07_425723 97 17 80 80 CD 10_1101-2021_01_07_425723 97 18 . . . 10_1101-2021_01_07_425723 98 1 No no DT 10_1101-2021_01_07_425723 98 2 164 164 CD 10_1101-2021_01_07_425723 98 3 further further JJ 10_1101-2021_01_07_425723 98 4 increase increase NN 10_1101-2021_01_07_425723 98 5 of of IN 10_1101-2021_01_07_425723 98 6 the the DT 10_1101-2021_01_07_425723 98 7 expression expression NN 10_1101-2021_01_07_425723 98 8 levels level NNS 10_1101-2021_01_07_425723 98 9 of of IN 10_1101-2021_01_07_425723 98 10 sterol sterol JJ 10_1101-2021_01_07_425723 98 11 biosynthetic biosynthetic JJ 10_1101-2021_01_07_425723 98 12 genes gene NNS 10_1101-2021_01_07_425723 98 13 was be VBD 10_1101-2021_01_07_425723 98 14 observed observe VBN 10_1101-2021_01_07_425723 98 15 upon upon IN 10_1101-2021_01_07_425723 98 16 omission omission NN 10_1101-2021_01_07_425723 98 17 of of IN 10_1101-2021_01_07_425723 98 18 165 165 CD 10_1101-2021_01_07_425723 98 19 these these DT 10_1101-2021_01_07_425723 98 20 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 98 21 growth growth NN 10_1101-2021_01_07_425723 98 22 factors factor NNS 10_1101-2021_01_07_425723 98 23 from from IN 10_1101-2021_01_07_425723 98 24 the the DT 10_1101-2021_01_07_425723 98 25 medium medium NN 10_1101-2021_01_07_425723 98 26 of of IN 10_1101-2021_01_07_425723 98 27 these these DT 10_1101-2021_01_07_425723 98 28 cultures culture NNS 10_1101-2021_01_07_425723 98 29 ( ( -LRB- 10_1101-2021_01_07_425723 98 30 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 98 31 Fig Fig NNP 10_1101-2021_01_07_425723 98 32 . . . 10_1101-2021_01_07_425723 99 1 6 6 CD 10_1101-2021_01_07_425723 99 2 , , , 10_1101-2021_01_07_425723 99 3 contrast contrast NN 10_1101-2021_01_07_425723 99 4 43 43 CD 10_1101-2021_01_07_425723 99 5 ) ) -RRB- 10_1101-2021_01_07_425723 99 6 . . . 10_1101-2021_01_07_425723 100 1 166 166 CD 10_1101-2021_01_07_425723 100 2 These these DT 10_1101-2021_01_07_425723 100 3 observations observation NNS 10_1101-2021_01_07_425723 100 4 suggested suggest VBD 10_1101-2021_01_07_425723 100 5 that that IN 10_1101-2021_01_07_425723 100 6 K. K. NNP 10_1101-2021_01_07_425723 100 7 marxianus marxianus NN 10_1101-2021_01_07_425723 100 8 may may MD 10_1101-2021_01_07_425723 100 9 be be VB 10_1101-2021_01_07_425723 100 10 unable unable JJ 10_1101-2021_01_07_425723 100 11 to to TO 10_1101-2021_01_07_425723 100 12 import import VB 10_1101-2021_01_07_425723 100 13 ergosterol ergosterol NN 10_1101-2021_01_07_425723 100 14 when when WRB 10_1101-2021_01_07_425723 100 15 sterol sterol NN 10_1101-2021_01_07_425723 100 16 167 167 CD 10_1101-2021_01_07_425723 100 17 synthesis synthesis NN 10_1101-2021_01_07_425723 100 18 is be VBZ 10_1101-2021_01_07_425723 100 19 compromised compromise VBN 10_1101-2021_01_07_425723 100 20 . . . 10_1101-2021_01_07_425723 101 1 Consistent consistent JJ 10_1101-2021_01_07_425723 101 2 with with IN 10_1101-2021_01_07_425723 101 3 this this DT 10_1101-2021_01_07_425723 101 4 hypothesis hypothesis NN 10_1101-2021_01_07_425723 101 5 , , , 10_1101-2021_01_07_425723 101 6 co co NN 10_1101-2021_01_07_425723 101 7 - - JJ 10_1101-2021_01_07_425723 101 8 orthology orthology JJ 10_1101-2021_01_07_425723 101 9 prediction prediction NN 10_1101-2021_01_07_425723 101 10 with with IN 10_1101-2021_01_07_425723 101 11 Proteinortho38 Proteinortho38 NNP 10_1101-2021_01_07_425723 101 12 168 168 CD 10_1101-2021_01_07_425723 101 13 revealed reveal VBD 10_1101-2021_01_07_425723 101 14 no no DT 10_1101-2021_01_07_425723 101 15 orthologs ortholog NNS 10_1101-2021_01_07_425723 101 16 of of IN 10_1101-2021_01_07_425723 101 17 the the DT 10_1101-2021_01_07_425723 101 18 S. S. NNP 10_1101-2021_01_07_425723 101 19 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 101 20 sterol sterol NN 10_1101-2021_01_07_425723 101 21 transporters transporter NNS 10_1101-2021_01_07_425723 101 22 Aus1 Aus1 NNP 10_1101-2021_01_07_425723 101 23 and and CC 10_1101-2021_01_07_425723 101 24 Pdr11 Pdr11 NNP 10_1101-2021_01_07_425723 101 25 in in IN 10_1101-2021_01_07_425723 101 26 K. K. NNP 10_1101-2021_01_07_425723 101 27 marxianus marxianus NN 10_1101-2021_01_07_425723 101 28 . . . 10_1101-2021_01_07_425723 102 1 169 169 CD 10_1101-2021_01_07_425723 102 2 K. K. NNP 10_1101-2021_01_07_425723 102 3 marxianus marxianus NN 10_1101-2021_01_07_425723 102 4 harbors harbor VBZ 10_1101-2021_01_07_425723 102 5 two two CD 10_1101-2021_01_07_425723 102 6 dihydroorotate dihydroorotate JJ 10_1101-2021_01_07_425723 102 7 dehydrogenases dehydrogenase NNS 10_1101-2021_01_07_425723 102 8 , , , 10_1101-2021_01_07_425723 102 9 a a DT 10_1101-2021_01_07_425723 102 10 cytosolic cytosolic JJ 10_1101-2021_01_07_425723 102 11 fumarate fumarate RB 10_1101-2021_01_07_425723 102 12 - - HYPH 10_1101-2021_01_07_425723 102 13 dependent dependent JJ 10_1101-2021_01_07_425723 102 14 enzyme enzyme NNS 10_1101-2021_01_07_425723 102 15 170 170 CD 10_1101-2021_01_07_425723 102 16 ( ( -LRB- 10_1101-2021_01_07_425723 102 17 KmUra1 KmUra1 NNP 10_1101-2021_01_07_425723 102 18 ) ) -RRB- 10_1101-2021_01_07_425723 102 19 and and CC 10_1101-2021_01_07_425723 102 20 a a DT 10_1101-2021_01_07_425723 102 21 mitochondrial mitochondrial NN 10_1101-2021_01_07_425723 102 22 quinone quinone NN 10_1101-2021_01_07_425723 102 23 - - HYPH 10_1101-2021_01_07_425723 102 24 dependent dependent JJ 10_1101-2021_01_07_425723 102 25 enzyme enzyme NNS 10_1101-2021_01_07_425723 102 26 ( ( -LRB- 10_1101-2021_01_07_425723 102 27 KmUra9 KmUra9 NNP 10_1101-2021_01_07_425723 102 28 ) ) -RRB- 10_1101-2021_01_07_425723 102 29 . . . 10_1101-2021_01_07_425723 103 1 In in IN 10_1101-2021_01_07_425723 103 2 vivo vivo NN 10_1101-2021_01_07_425723 103 3 activity activity NN 10_1101-2021_01_07_425723 103 4 of of IN 10_1101-2021_01_07_425723 103 5 the the DT 10_1101-2021_01_07_425723 103 6 latter latter JJ 10_1101-2021_01_07_425723 103 7 171 171 CD 10_1101-2021_01_07_425723 103 8 requires require VBZ 10_1101-2021_01_07_425723 103 9 oxygen oxygen NN 10_1101-2021_01_07_425723 103 10 because because IN 10_1101-2021_01_07_425723 103 11 the the DT 10_1101-2021_01_07_425723 103 12 reduced reduced JJ 10_1101-2021_01_07_425723 103 13 quinone quinone NN 10_1101-2021_01_07_425723 103 14 is be VBZ 10_1101-2021_01_07_425723 103 15 reoxidized reoxidize VBN 10_1101-2021_01_07_425723 103 16 by by IN 10_1101-2021_01_07_425723 103 17 the the DT 10_1101-2021_01_07_425723 103 18 mitochondrial mitochondrial NN 10_1101-2021_01_07_425723 103 19 respiratory respiratory JJ 10_1101-2021_01_07_425723 103 20 chain39 chain39 NNP 10_1101-2021_01_07_425723 103 21 . . . 10_1101-2021_01_07_425723 104 1 172 172 CD 10_1101-2021_01_07_425723 104 2 Consistent consistent JJ 10_1101-2021_01_07_425723 104 3 with with IN 10_1101-2021_01_07_425723 104 4 these these DT 10_1101-2021_01_07_425723 104 5 different different JJ 10_1101-2021_01_07_425723 104 6 oxygen oxygen NN 10_1101-2021_01_07_425723 104 7 requirements requirement NNS 10_1101-2021_01_07_425723 104 8 , , , 10_1101-2021_01_07_425723 104 9 KmURA9 KmURA9 NNP 10_1101-2021_01_07_425723 104 10 was be VBD 10_1101-2021_01_07_425723 104 11 down down RB 10_1101-2021_01_07_425723 104 12 - - HYPH 10_1101-2021_01_07_425723 104 13 regulated regulated JJ 10_1101-2021_01_07_425723 104 14 under under IN 10_1101-2021_01_07_425723 104 15 severely severely RB 10_1101-2021_01_07_425723 104 16 173 173 CD 10_1101-2021_01_07_425723 104 17 oxygen oxygen NN 10_1101-2021_01_07_425723 104 18 - - HYPH 10_1101-2021_01_07_425723 104 19 limited limit VBN 10_1101-2021_01_07_425723 104 20 conditions condition NNS 10_1101-2021_01_07_425723 104 21 , , , 10_1101-2021_01_07_425723 104 22 while while IN 10_1101-2021_01_07_425723 104 23 KmURA1 KmURA1 NNP 10_1101-2021_01_07_425723 104 24 was be VBD 10_1101-2021_01_07_425723 104 25 upregulated upregulated JJ 10_1101-2021_01_07_425723 104 26 ( ( -LRB- 10_1101-2021_01_07_425723 104 27 Fig fig NN 10_1101-2021_01_07_425723 104 28 . . . 10_1101-2021_01_07_425723 105 1 2b 2b NNP 10_1101-2021_01_07_425723 105 2 , , , 10_1101-2021_01_07_425723 105 3 contrast contrast NN 10_1101-2021_01_07_425723 105 4 31 31 CD 10_1101-2021_01_07_425723 105 5 ) ) -RRB- 10_1101-2021_01_07_425723 105 6 . . . 10_1101-2021_01_07_425723 106 1 Upregulation upregulation NN 10_1101-2021_01_07_425723 106 2 of of IN 10_1101-2021_01_07_425723 106 3 174 174 CD 10_1101-2021_01_07_425723 106 4 KmURA1 KmURA1 NNP 10_1101-2021_01_07_425723 106 5 coincided coincide VBN 10_1101-2021_01_07_425723 106 6 with with IN 10_1101-2021_01_07_425723 106 7 increased increase VBN 10_1101-2021_01_07_425723 106 8 production production NN 10_1101-2021_01_07_425723 106 9 of of IN 10_1101-2021_01_07_425723 106 10 succinate succinate NN 10_1101-2021_01_07_425723 106 11 ( ( -LRB- 10_1101-2021_01_07_425723 106 12 Table table NN 10_1101-2021_01_07_425723 106 13 1 1 CD 10_1101-2021_01_07_425723 106 14 ) ) -RRB- 10_1101-2021_01_07_425723 106 15 . . . 10_1101-2021_01_07_425723 107 1 175 175 CD 10_1101-2021_01_07_425723 107 2 .CC .CC : 10_1101-2021_01_07_425723 107 3 - - HYPH 10_1101-2021_01_07_425723 107 4 BY by IN 10_1101-2021_01_07_425723 107 5 - - HYPH 10_1101-2021_01_07_425723 107 6 NC NC NNP 10_1101-2021_01_07_425723 107 7 - - HYPH 10_1101-2021_01_07_425723 107 8 ND ND NNP 10_1101-2021_01_07_425723 107 9 4.0 4.0 CD 10_1101-2021_01_07_425723 107 10 International International NNP 10_1101-2021_01_07_425723 107 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 107 12 under under IN 10_1101-2021_01_07_425723 107 13 a a DT 10_1101-2021_01_07_425723 107 14 ( ( -LRB- 10_1101-2021_01_07_425723 107 15 which which WDT 10_1101-2021_01_07_425723 107 16 was be VBD 10_1101-2021_01_07_425723 107 17 not not RB 10_1101-2021_01_07_425723 107 18 certified certify VBN 10_1101-2021_01_07_425723 107 19 by by IN 10_1101-2021_01_07_425723 107 20 peer peer NN 10_1101-2021_01_07_425723 107 21 review review NN 10_1101-2021_01_07_425723 107 22 ) ) -RRB- 10_1101-2021_01_07_425723 107 23 is be VBZ 10_1101-2021_01_07_425723 107 24 the the DT 10_1101-2021_01_07_425723 107 25 author author NN 10_1101-2021_01_07_425723 107 26 / / SYM 10_1101-2021_01_07_425723 107 27 funder funder NN 10_1101-2021_01_07_425723 107 28 , , , 10_1101-2021_01_07_425723 107 29 who who WP 10_1101-2021_01_07_425723 107 30 has have VBZ 10_1101-2021_01_07_425723 107 31 granted grant VBN 10_1101-2021_01_07_425723 107 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 107 33 a a DT 10_1101-2021_01_07_425723 107 34 license license NN 10_1101-2021_01_07_425723 107 35 to to TO 10_1101-2021_01_07_425723 107 36 display display VB 10_1101-2021_01_07_425723 107 37 the the DT 10_1101-2021_01_07_425723 107 38 preprint preprint NN 10_1101-2021_01_07_425723 107 39 in in IN 10_1101-2021_01_07_425723 107 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 107 41 . . . 10_1101-2021_01_07_425723 108 1 It -PRON- PRP 10_1101-2021_01_07_425723 108 2 is be VBZ 10_1101-2021_01_07_425723 108 3 made make VBN 10_1101-2021_01_07_425723 108 4 The the DT 10_1101-2021_01_07_425723 108 5 copyright copyright NN 10_1101-2021_01_07_425723 108 6 holder holder NN 10_1101-2021_01_07_425723 108 7 for for IN 10_1101-2021_01_07_425723 108 8 this this DT 10_1101-2021_01_07_425723 108 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 108 10 version version NN 10_1101-2021_01_07_425723 108 11 posted post VBD 10_1101-2021_01_07_425723 108 12 January January NNP 10_1101-2021_01_07_425723 108 13 8 8 CD 10_1101-2021_01_07_425723 108 14 , , , 10_1101-2021_01_07_425723 108 15 2021 2021 CD 10_1101-2021_01_07_425723 108 16 . . . 10_1101-2021_01_07_425723 108 17 ; ; : 10_1101-2021_01_07_425723 108 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 108 19 : : : 10_1101-2021_01_07_425723 108 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 108 21 preprint preprint NN 10_1101-2021_01_07_425723 108 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 108 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 108 24 11 11 CD 10_1101-2021_01_07_425723 108 25 Fig Fig NNP 10_1101-2021_01_07_425723 108 26 . . . 10_1101-2021_01_07_425723 109 1 2 2 CD 10_1101-2021_01_07_425723 109 2 | | JJ 10_1101-2021_01_07_425723 109 3 Transcriptional transcriptional JJ 10_1101-2021_01_07_425723 109 4 response response NN 10_1101-2021_01_07_425723 109 5 of of IN 10_1101-2021_01_07_425723 109 6 K. K. NNP 10_1101-2021_01_07_425723 109 7 marxianus marxianus NN 10_1101-2021_01_07_425723 109 8 and and CC 10_1101-2021_01_07_425723 109 9 S. S. NNP 10_1101-2021_01_07_425723 109 10 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 109 11 to to TO 10_1101-2021_01_07_425723 109 12 oxygen oxygen VB 10_1101-2021_01_07_425723 109 13 limitation limitation NN 10_1101-2021_01_07_425723 109 14 and and CC 10_1101-2021_01_07_425723 109 15 sterol sterol NN 10_1101-2021_01_07_425723 109 16 , , , 10_1101-2021_01_07_425723 109 17 176 176 CD 10_1101-2021_01_07_425723 109 18 Tween Tween NNP 10_1101-2021_01_07_425723 109 19 80 80 CD 10_1101-2021_01_07_425723 109 20 supplementation supplementation NN 10_1101-2021_01_07_425723 109 21 . . . 10_1101-2021_01_07_425723 110 1 Transcriptome transcriptome DT 10_1101-2021_01_07_425723 110 2 analyses analysis NNS 10_1101-2021_01_07_425723 110 3 were be VBD 10_1101-2021_01_07_425723 110 4 performed perform VBN 10_1101-2021_01_07_425723 110 5 for for IN 10_1101-2021_01_07_425723 110 6 each each DT 10_1101-2021_01_07_425723 110 7 cultivation cultivation NN 10_1101-2021_01_07_425723 110 8 regime regime NN 10_1101-2021_01_07_425723 110 9 ( ( -LRB- 10_1101-2021_01_07_425723 110 10 1 1 CD 10_1101-2021_01_07_425723 110 11 to to IN 10_1101-2021_01_07_425723 110 12 177 177 CD 10_1101-2021_01_07_425723 110 13 5 5 CD 10_1101-2021_01_07_425723 110 14 ) ) -RRB- 10_1101-2021_01_07_425723 110 15 of of IN 10_1101-2021_01_07_425723 110 16 S. S. NNP 10_1101-2021_01_07_425723 110 17 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 110 18 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 110 19 - - HYPH 10_1101-2021_01_07_425723 110 20 7D 7D NNP 10_1101-2021_01_07_425723 110 21 ( ( -LRB- 10_1101-2021_01_07_425723 110 22 scer scer NN 10_1101-2021_01_07_425723 110 23 ) ) -RRB- 10_1101-2021_01_07_425723 110 24 and and CC 10_1101-2021_01_07_425723 110 25 K. K. NNP 10_1101-2021_01_07_425723 110 26 marxianus marxianus NN 10_1101-2021_01_07_425723 110 27 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 110 28 ( ( -LRB- 10_1101-2021_01_07_425723 110 29 kmar kmar NNP 10_1101-2021_01_07_425723 110 30 ) ) -RRB- 10_1101-2021_01_07_425723 110 31 . . . 10_1101-2021_01_07_425723 111 1 Data datum NNS 10_1101-2021_01_07_425723 111 2 for for IN 10_1101-2021_01_07_425723 111 3 each each DT 10_1101-2021_01_07_425723 111 4 regime regime NN 10_1101-2021_01_07_425723 111 5 were be VBD 10_1101-2021_01_07_425723 111 6 178 178 CD 10_1101-2021_01_07_425723 111 7 obtained obtain VBN 10_1101-2021_01_07_425723 111 8 from from IN 10_1101-2021_01_07_425723 111 9 independent independent JJ 10_1101-2021_01_07_425723 111 10 replicate replicate NN 10_1101-2021_01_07_425723 111 11 chemostat chemostat JJ 10_1101-2021_01_07_425723 111 12 cultures culture NNS 10_1101-2021_01_07_425723 111 13 ( ( -LRB- 10_1101-2021_01_07_425723 111 14 Fig fig NN 10_1101-2021_01_07_425723 111 15 . . . 10_1101-2021_01_07_425723 112 1 1 1 LS 10_1101-2021_01_07_425723 112 2 ) ) -RRB- 10_1101-2021_01_07_425723 112 3 . . . 10_1101-2021_01_07_425723 113 1 a a LS 10_1101-2021_01_07_425723 113 2 , , , 10_1101-2021_01_07_425723 113 3 Comparison Comparison NNP 10_1101-2021_01_07_425723 113 4 of of IN 10_1101-2021_01_07_425723 113 5 GO GO NNP 10_1101-2021_01_07_425723 113 6 - - HYPH 10_1101-2021_01_07_425723 113 7 term term NN 10_1101-2021_01_07_425723 113 8 gene gene NN 10_1101-2021_01_07_425723 113 9 - - HYPH 10_1101-2021_01_07_425723 113 10 set set VBN 10_1101-2021_01_07_425723 113 11 179 179 CD 10_1101-2021_01_07_425723 113 12 enrichment enrichment NN 10_1101-2021_01_07_425723 113 13 analysis analysis NN 10_1101-2021_01_07_425723 113 14 of of IN 10_1101-2021_01_07_425723 113 15 biological biological JJ 10_1101-2021_01_07_425723 113 16 processes process NNS 10_1101-2021_01_07_425723 113 17 in in IN 10_1101-2021_01_07_425723 113 18 contrast contrast NN 10_1101-2021_01_07_425723 113 19 31 31 CD 10_1101-2021_01_07_425723 113 20 of of IN 10_1101-2021_01_07_425723 113 21 S. S. NNP 10_1101-2021_01_07_425723 113 22 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 113 23 and and CC 10_1101-2021_01_07_425723 113 24 K. K. NNP 10_1101-2021_01_07_425723 113 25 marxianus marxianus NN 10_1101-2021_01_07_425723 113 26 with with IN 10_1101-2021_01_07_425723 113 27 short short JJ 10_1101-2021_01_07_425723 113 28 180 180 CD 10_1101-2021_01_07_425723 113 29 description description NN 10_1101-2021_01_07_425723 113 30 of of IN 10_1101-2021_01_07_425723 113 31 GO go NN 10_1101-2021_01_07_425723 113 32 - - HYPH 10_1101-2021_01_07_425723 113 33 terms term NNS 10_1101-2021_01_07_425723 113 34 ( ( -LRB- 10_1101-2021_01_07_425723 113 35 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 113 36 Fig Fig NNP 10_1101-2021_01_07_425723 113 37 . . . 10_1101-2021_01_07_425723 114 1 2 2 LS 10_1101-2021_01_07_425723 114 2 - - SYM 10_1101-2021_01_07_425723 114 3 5 5 CD 10_1101-2021_01_07_425723 114 4 ) ) -RRB- 10_1101-2021_01_07_425723 114 5 . . . 10_1101-2021_01_07_425723 115 1 GO go NN 10_1101-2021_01_07_425723 115 2 - - HYPH 10_1101-2021_01_07_425723 115 3 terms term NNS 10_1101-2021_01_07_425723 115 4 were be VBD 10_1101-2021_01_07_425723 115 5 vertically vertically RB 10_1101-2021_01_07_425723 115 6 ordered order VBN 10_1101-2021_01_07_425723 115 7 based base VBN 10_1101-2021_01_07_425723 115 8 on on IN 10_1101-2021_01_07_425723 115 9 their -PRON- PRP$ 10_1101-2021_01_07_425723 115 10 181 181 CD 10_1101-2021_01_07_425723 115 11 distinct distinct JJ 10_1101-2021_01_07_425723 115 12 directionality directionality NN 10_1101-2021_01_07_425723 115 13 calculated calculate VBN 10_1101-2021_01_07_425723 115 14 with with IN 10_1101-2021_01_07_425723 115 15 Piano40 Piano40 NNP 10_1101-2021_01_07_425723 115 16 with with IN 10_1101-2021_01_07_425723 115 17 GO GO NNP 10_1101-2021_01_07_425723 115 18 - - HYPH 10_1101-2021_01_07_425723 115 19 terms term NNS 10_1101-2021_01_07_425723 115 20 enriched enrich VBN 10_1101-2021_01_07_425723 115 21 solely solely RB 10_1101-2021_01_07_425723 115 22 with with IN 10_1101-2021_01_07_425723 115 23 up up RB 10_1101-2021_01_07_425723 115 24 - - HYPH 10_1101-2021_01_07_425723 115 25 regulated regulate VBN 10_1101-2021_01_07_425723 115 26 genes gene NNS 10_1101-2021_01_07_425723 115 27 182 182 CD 10_1101-2021_01_07_425723 115 28 ( ( -LRB- 10_1101-2021_01_07_425723 115 29 blue blue NNP 10_1101-2021_01_07_425723 115 30 ) ) -RRB- 10_1101-2021_01_07_425723 115 31 at at IN 10_1101-2021_01_07_425723 115 32 the the DT 10_1101-2021_01_07_425723 115 33 top top NN 10_1101-2021_01_07_425723 115 34 , , , 10_1101-2021_01_07_425723 115 35 GO GO NNP 10_1101-2021_01_07_425723 115 36 - - HYPH 10_1101-2021_01_07_425723 115 37 terms term NNS 10_1101-2021_01_07_425723 115 38 with with IN 10_1101-2021_01_07_425723 115 39 mixed- mixed- NNP 10_1101-2021_01_07_425723 115 40 or or CC 10_1101-2021_01_07_425723 115 41 no no DT 10_1101-2021_01_07_425723 115 42 - - HYPH 10_1101-2021_01_07_425723 115 43 directionality directionality NN 10_1101-2021_01_07_425723 115 44 in in IN 10_1101-2021_01_07_425723 115 45 the the DT 10_1101-2021_01_07_425723 115 46 middle middle NNP 10_1101-2021_01_07_425723 115 47 ( ( -LRB- 10_1101-2021_01_07_425723 115 48 white white NNP 10_1101-2021_01_07_425723 115 49 ) ) -RRB- 10_1101-2021_01_07_425723 115 50 and and CC 10_1101-2021_01_07_425723 115 51 GO GO NNP 10_1101-2021_01_07_425723 115 52 - - HYPH 10_1101-2021_01_07_425723 115 53 terms term NNS 10_1101-2021_01_07_425723 115 54 with with IN 10_1101-2021_01_07_425723 115 55 183 183 CD 10_1101-2021_01_07_425723 115 56 solely solely RB 10_1101-2021_01_07_425723 115 57 down down RB 10_1101-2021_01_07_425723 115 58 - - HYPH 10_1101-2021_01_07_425723 115 59 regulated regulate VBN 10_1101-2021_01_07_425723 115 60 genes gene NNS 10_1101-2021_01_07_425723 115 61 at at IN 10_1101-2021_01_07_425723 115 62 the the DT 10_1101-2021_01_07_425723 115 63 bottom bottom NN 10_1101-2021_01_07_425723 115 64 ( ( -LRB- 10_1101-2021_01_07_425723 115 65 brown brown NNP 10_1101-2021_01_07_425723 115 66 ) ) -RRB- 10_1101-2021_01_07_425723 115 67 . . . 10_1101-2021_01_07_425723 116 1 b b NNP 10_1101-2021_01_07_425723 116 2 , , , 10_1101-2021_01_07_425723 116 3 c c NNP 10_1101-2021_01_07_425723 116 4 , , , 10_1101-2021_01_07_425723 116 5 d d NNP 10_1101-2021_01_07_425723 116 6 , , , 10_1101-2021_01_07_425723 116 7 Subsets Subsets NNPS 10_1101-2021_01_07_425723 116 8 of of IN 10_1101-2021_01_07_425723 116 9 differentially differentially RB 10_1101-2021_01_07_425723 116 10 expressed express VBD 10_1101-2021_01_07_425723 116 11 184 184 CD 10_1101-2021_01_07_425723 116 12 orthologous orthologous JJ 10_1101-2021_01_07_425723 116 13 genes gene NNS 10_1101-2021_01_07_425723 116 14 obtained obtain VBN 10_1101-2021_01_07_425723 116 15 from from IN 10_1101-2021_01_07_425723 116 16 the the DT 10_1101-2021_01_07_425723 116 17 gene gene NN 10_1101-2021_01_07_425723 116 18 - - HYPH 10_1101-2021_01_07_425723 116 19 set set VBN 10_1101-2021_01_07_425723 116 20 analyses analysis NNS 10_1101-2021_01_07_425723 116 21 for for IN 10_1101-2021_01_07_425723 116 22 both both DT 10_1101-2021_01_07_425723 116 23 yeasts yeast NNS 10_1101-2021_01_07_425723 116 24 in in IN 10_1101-2021_01_07_425723 116 25 contrasts contrasts NNP 10_1101-2021_01_07_425723 116 26 31 31 CD 10_1101-2021_01_07_425723 116 27 and and CC 10_1101-2021_01_07_425723 116 28 43 43 CD 10_1101-2021_01_07_425723 116 29 , , , 10_1101-2021_01_07_425723 116 30 and and CC 10_1101-2021_01_07_425723 116 31 with with IN 10_1101-2021_01_07_425723 116 32 185 185 CD 10_1101-2021_01_07_425723 116 33 genes gene NNS 10_1101-2021_01_07_425723 116 34 without without IN 10_1101-2021_01_07_425723 116 35 orthologs ortholog NNS 10_1101-2021_01_07_425723 116 36 depicted depict VBN 10_1101-2021_01_07_425723 116 37 with with IN 10_1101-2021_01_07_425723 116 38 logFC logfc IN 10_1101-2021_01_07_425723 116 39 value value NN 10_1101-2021_01_07_425723 116 40 of of IN 10_1101-2021_01_07_425723 116 41 0 0 CD 10_1101-2021_01_07_425723 116 42 in in IN 10_1101-2021_01_07_425723 116 43 the the DT 10_1101-2021_01_07_425723 116 44 respective respective JJ 10_1101-2021_01_07_425723 116 45 yeast yeast NN 10_1101-2021_01_07_425723 116 46 . . . 10_1101-2021_01_07_425723 117 1 b b LS 10_1101-2021_01_07_425723 117 2 , , , 10_1101-2021_01_07_425723 117 3 S. S. NNP 10_1101-2021_01_07_425723 117 4 cerevisiae cerevisiae NN 10_1101-2021_01_07_425723 117 5 genes gene NNS 10_1101-2021_01_07_425723 117 6 186 186 CD 10_1101-2021_01_07_425723 117 7 previously previously RB 10_1101-2021_01_07_425723 117 8 shown show VBN 10_1101-2021_01_07_425723 117 9 as as IN 10_1101-2021_01_07_425723 117 10 consistently consistently RB 10_1101-2021_01_07_425723 117 11 upregulated upregulate VBN 10_1101-2021_01_07_425723 117 12 under under IN 10_1101-2021_01_07_425723 117 13 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 117 14 conditions condition NNS 10_1101-2021_01_07_425723 117 15 in in IN 10_1101-2021_01_07_425723 117 16 four four CD 10_1101-2021_01_07_425723 117 17 different different JJ 10_1101-2021_01_07_425723 117 18 nutrient-187 nutrient-187 HYPH 10_1101-2021_01_07_425723 117 19 .CC .CC , 10_1101-2021_01_07_425723 117 20 - - HYPH 10_1101-2021_01_07_425723 117 21 BY by IN 10_1101-2021_01_07_425723 117 22 - - HYPH 10_1101-2021_01_07_425723 117 23 NC NC NNP 10_1101-2021_01_07_425723 117 24 - - HYPH 10_1101-2021_01_07_425723 117 25 ND ND NNP 10_1101-2021_01_07_425723 117 26 4.0 4.0 CD 10_1101-2021_01_07_425723 117 27 International International NNP 10_1101-2021_01_07_425723 117 28 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 117 29 under under IN 10_1101-2021_01_07_425723 117 30 a a DT 10_1101-2021_01_07_425723 117 31 ( ( -LRB- 10_1101-2021_01_07_425723 117 32 which which WDT 10_1101-2021_01_07_425723 117 33 was be VBD 10_1101-2021_01_07_425723 117 34 not not RB 10_1101-2021_01_07_425723 117 35 certified certify VBN 10_1101-2021_01_07_425723 117 36 by by IN 10_1101-2021_01_07_425723 117 37 peer peer NN 10_1101-2021_01_07_425723 117 38 review review NN 10_1101-2021_01_07_425723 117 39 ) ) -RRB- 10_1101-2021_01_07_425723 117 40 is be VBZ 10_1101-2021_01_07_425723 117 41 the the DT 10_1101-2021_01_07_425723 117 42 author author NN 10_1101-2021_01_07_425723 117 43 / / SYM 10_1101-2021_01_07_425723 117 44 funder funder NN 10_1101-2021_01_07_425723 117 45 , , , 10_1101-2021_01_07_425723 117 46 who who WP 10_1101-2021_01_07_425723 117 47 has have VBZ 10_1101-2021_01_07_425723 117 48 granted grant VBN 10_1101-2021_01_07_425723 117 49 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 117 50 a a DT 10_1101-2021_01_07_425723 117 51 license license NN 10_1101-2021_01_07_425723 117 52 to to TO 10_1101-2021_01_07_425723 117 53 display display VB 10_1101-2021_01_07_425723 117 54 the the DT 10_1101-2021_01_07_425723 117 55 preprint preprint NN 10_1101-2021_01_07_425723 117 56 in in IN 10_1101-2021_01_07_425723 117 57 perpetuity perpetuity NN 10_1101-2021_01_07_425723 117 58 . . . 10_1101-2021_01_07_425723 118 1 It -PRON- PRP 10_1101-2021_01_07_425723 118 2 is be VBZ 10_1101-2021_01_07_425723 118 3 made make VBN 10_1101-2021_01_07_425723 118 4 The the DT 10_1101-2021_01_07_425723 118 5 copyright copyright NN 10_1101-2021_01_07_425723 118 6 holder holder NN 10_1101-2021_01_07_425723 118 7 for for IN 10_1101-2021_01_07_425723 118 8 this this DT 10_1101-2021_01_07_425723 118 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 118 10 version version NN 10_1101-2021_01_07_425723 118 11 posted post VBD 10_1101-2021_01_07_425723 118 12 January January NNP 10_1101-2021_01_07_425723 118 13 8 8 CD 10_1101-2021_01_07_425723 118 14 , , , 10_1101-2021_01_07_425723 118 15 2021 2021 CD 10_1101-2021_01_07_425723 118 16 . . . 10_1101-2021_01_07_425723 118 17 ; ; : 10_1101-2021_01_07_425723 118 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 118 19 : : : 10_1101-2021_01_07_425723 118 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 118 21 preprint preprint NN 10_1101-2021_01_07_425723 118 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 118 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425723 118 24 12 12 CD 10_1101-2021_01_07_425723 118 25 limitations41 limitations41 CD 10_1101-2021_01_07_425723 118 26 . . . 10_1101-2021_01_07_425723 119 1 c c LS 10_1101-2021_01_07_425723 119 2 , , , 10_1101-2021_01_07_425723 119 3 As as IN 10_1101-2021_01_07_425723 119 4 described describe VBN 10_1101-2021_01_07_425723 119 5 for for IN 10_1101-2021_01_07_425723 119 6 panel panel NN 10_1101-2021_01_07_425723 119 7 b b NNP 10_1101-2021_01_07_425723 119 8 but but CC 10_1101-2021_01_07_425723 119 9 for for IN 10_1101-2021_01_07_425723 119 10 downregulated downregulated JJ 10_1101-2021_01_07_425723 119 11 genes gene NNS 10_1101-2021_01_07_425723 119 12 . . . 10_1101-2021_01_07_425723 120 1 d d LS 10_1101-2021_01_07_425723 120 2 , , , 10_1101-2021_01_07_425723 120 3 Differentially Differentially NNP 10_1101-2021_01_07_425723 120 4 expressed express VBD 10_1101-2021_01_07_425723 120 5 genes gene NNS 10_1101-2021_01_07_425723 120 6 188 188 CD 10_1101-2021_01_07_425723 120 7 uniquely uniquely RB 10_1101-2021_01_07_425723 120 8 found find VBN 10_1101-2021_01_07_425723 120 9 in in IN 10_1101-2021_01_07_425723 120 10 this this DT 10_1101-2021_01_07_425723 120 11 study study NN 10_1101-2021_01_07_425723 120 12 . . . 10_1101-2021_01_07_425723 121 1 e e NNP 10_1101-2021_01_07_425723 121 2 , , , 10_1101-2021_01_07_425723 121 3 f f NNP 10_1101-2021_01_07_425723 121 4 , , , 10_1101-2021_01_07_425723 121 5 g g NNP 10_1101-2021_01_07_425723 121 6 , , , 10_1101-2021_01_07_425723 121 7 h h NN 10_1101-2021_01_07_425723 121 8 , , , 10_1101-2021_01_07_425723 121 9 Highlighted highlight VBN 10_1101-2021_01_07_425723 121 10 gene gene NN 10_1101-2021_01_07_425723 121 11 - - HYPH 10_1101-2021_01_07_425723 121 12 sets set NNS 10_1101-2021_01_07_425723 121 13 showing show VBG 10_1101-2021_01_07_425723 121 14 divergent divergent JJ 10_1101-2021_01_07_425723 121 15 expression expression NN 10_1101-2021_01_07_425723 121 16 patterns pattern NNS 10_1101-2021_01_07_425723 121 17 189 189 CD 10_1101-2021_01_07_425723 121 18 across across IN 10_1101-2021_01_07_425723 121 19 the the DT 10_1101-2021_01_07_425723 121 20 two two CD 10_1101-2021_01_07_425723 121 21 yeasts yeast NNS 10_1101-2021_01_07_425723 121 22 . . . 10_1101-2021_01_07_425723 122 1 e e LS 10_1101-2021_01_07_425723 122 2 , , , 10_1101-2021_01_07_425723 122 3 S. S. NNP 10_1101-2021_01_07_425723 122 4 cerevisiae cerevisiae NN 10_1101-2021_01_07_425723 122 5 genes gene NNS 10_1101-2021_01_07_425723 122 6 upregulated upregulate VBN 10_1101-2021_01_07_425723 122 7 in in IN 10_1101-2021_01_07_425723 122 8 contrast contrast NN 10_1101-2021_01_07_425723 122 9 31 31 CD 10_1101-2021_01_07_425723 122 10 but but CC 10_1101-2021_01_07_425723 122 11 downregulated downregulate VBN 10_1101-2021_01_07_425723 122 12 in in IN 10_1101-2021_01_07_425723 122 13 K. K. NNP 10_1101-2021_01_07_425723 122 14 190 190 CD 10_1101-2021_01_07_425723 122 15 marxianus marxianus NN 10_1101-2021_01_07_425723 122 16 . . . 10_1101-2021_01_07_425723 123 1 f f LS 10_1101-2021_01_07_425723 123 2 , , , 10_1101-2021_01_07_425723 123 3 S. S. NNP 10_1101-2021_01_07_425723 123 4 cerevisiae cerevisiae NN 10_1101-2021_01_07_425723 123 5 genes gene NNS 10_1101-2021_01_07_425723 123 6 downregulated downregulate VBN 10_1101-2021_01_07_425723 123 7 in in IN 10_1101-2021_01_07_425723 123 8 contrast contrast NN 10_1101-2021_01_07_425723 123 9 31 31 CD 10_1101-2021_01_07_425723 123 10 but but CC 10_1101-2021_01_07_425723 123 11 upregulated upregulated JJ 10_1101-2021_01_07_425723 123 12 in in IN 10_1101-2021_01_07_425723 123 13 K. K. NNP 10_1101-2021_01_07_425723 123 14 marxianus marxianus NN 10_1101-2021_01_07_425723 123 15 . . . 10_1101-2021_01_07_425723 124 1 g g NNP 10_1101-2021_01_07_425723 124 2 , , , 10_1101-2021_01_07_425723 124 3 h h NN 10_1101-2021_01_07_425723 124 4 , , , 10_1101-2021_01_07_425723 124 5 191 191 CD 10_1101-2021_01_07_425723 124 6 Similar similar JJ 10_1101-2021_01_07_425723 124 7 to to IN 10_1101-2021_01_07_425723 124 8 e e NN 10_1101-2021_01_07_425723 124 9 and and CC 10_1101-2021_01_07_425723 124 10 f f NNP 10_1101-2021_01_07_425723 124 11 but but CC 10_1101-2021_01_07_425723 124 12 for for IN 10_1101-2021_01_07_425723 124 13 contrast contrast NN 10_1101-2021_01_07_425723 124 14 43 43 CD 10_1101-2021_01_07_425723 124 15 . . . 10_1101-2021_01_07_425723 125 1 192 192 CD 10_1101-2021_01_07_425723 125 2 Fig fig NN 10_1101-2021_01_07_425723 125 3 . . . 10_1101-2021_01_07_425723 126 1 3 3 CD 10_1101-2021_01_07_425723 126 2 | | NNP 10_1101-2021_01_07_425723 126 3 Different different JJ 10_1101-2021_01_07_425723 126 4 transcriptional transcriptional JJ 10_1101-2021_01_07_425723 126 5 regulation regulation NN 10_1101-2021_01_07_425723 126 6 of of IN 10_1101-2021_01_07_425723 126 7 ergosterol ergosterol JJ 10_1101-2021_01_07_425723 126 8 - - HYPH 10_1101-2021_01_07_425723 126 9 biosynthesis biosynthesis NN 10_1101-2021_01_07_425723 126 10 in in IN 10_1101-2021_01_07_425723 126 11 K. K. NNP 10_1101-2021_01_07_425723 126 12 marxianus marxianus NN 10_1101-2021_01_07_425723 126 13 and and CC 10_1101-2021_01_07_425723 126 14 S. S. NNP 10_1101-2021_01_07_425723 126 15 193 193 CD 10_1101-2021_01_07_425723 126 16 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 126 17 . . . 10_1101-2021_01_07_425723 127 1 a a LS 10_1101-2021_01_07_425723 127 2 , , , 10_1101-2021_01_07_425723 127 3 RNAseq RNAseq NNP 10_1101-2021_01_07_425723 127 4 was be VBD 10_1101-2021_01_07_425723 127 5 performed perform VBN 10_1101-2021_01_07_425723 127 6 on on IN 10_1101-2021_01_07_425723 127 7 independent independent JJ 10_1101-2021_01_07_425723 127 8 replicate replicate NN 10_1101-2021_01_07_425723 127 9 chemostat chemostat JJ 10_1101-2021_01_07_425723 127 10 cultures culture NNS 10_1101-2021_01_07_425723 127 11 of of IN 10_1101-2021_01_07_425723 127 12 S. S. NNP 10_1101-2021_01_07_425723 127 13 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 127 14 194 194 CD 10_1101-2021_01_07_425723 127 15 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 127 16 - - HYPH 10_1101-2021_01_07_425723 127 17 7D 7D NNP 10_1101-2021_01_07_425723 127 18 and and CC 10_1101-2021_01_07_425723 127 19 K. K. NNP 10_1101-2021_01_07_425723 127 20 marxianus marxianus NN 10_1101-2021_01_07_425723 127 21 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 127 22 for for IN 10_1101-2021_01_07_425723 127 23 each each DT 10_1101-2021_01_07_425723 127 24 aeration aeration NN 10_1101-2021_01_07_425723 127 25 and and CC 10_1101-2021_01_07_425723 127 26 anaerobic anaerobic NN 10_1101-2021_01_07_425723 127 27 - - HYPH 10_1101-2021_01_07_425723 127 28 growth growth NN 10_1101-2021_01_07_425723 127 29 - - HYPH 10_1101-2021_01_07_425723 127 30 factor factor NN 10_1101-2021_01_07_425723 127 31 195 195 CD 10_1101-2021_01_07_425723 127 32 supplementation supplementation NN 10_1101-2021_01_07_425723 127 33 regime regime NN 10_1101-2021_01_07_425723 127 34 ( ( -LRB- 10_1101-2021_01_07_425723 127 35 1 1 CD 10_1101-2021_01_07_425723 127 36 to to IN 10_1101-2021_01_07_425723 127 37 5 5 CD 10_1101-2021_01_07_425723 127 38 ; ; : 10_1101-2021_01_07_425723 127 39 Fig Fig NNP 10_1101-2021_01_07_425723 127 40 . . . 10_1101-2021_01_07_425723 128 1 1 1 LS 10_1101-2021_01_07_425723 128 2 ) ) -RRB- 10_1101-2021_01_07_425723 128 3 . . . 10_1101-2021_01_07_425723 129 1 b b LS 10_1101-2021_01_07_425723 129 2 , , , 10_1101-2021_01_07_425723 129 3 Transcriptional transcriptional JJ 10_1101-2021_01_07_425723 129 4 differences difference NNS 10_1101-2021_01_07_425723 129 5 in in IN 10_1101-2021_01_07_425723 129 6 the the DT 10_1101-2021_01_07_425723 129 7 mevalonate- mevalonate- NNS 10_1101-2021_01_07_425723 129 8 and and CC 10_1101-2021_01_07_425723 129 9 196 196 CD 10_1101-2021_01_07_425723 129 10 ergosterol ergosterol JJ 10_1101-2021_01_07_425723 129 11 - - HYPH 10_1101-2021_01_07_425723 129 12 pathway pathway NN 10_1101-2021_01_07_425723 129 13 genes gene NNS 10_1101-2021_01_07_425723 129 14 of of IN 10_1101-2021_01_07_425723 129 15 S. S. NNP 10_1101-2021_01_07_425723 129 16 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 129 17 and and CC 10_1101-2021_01_07_425723 129 18 K. K. NNP 10_1101-2021_01_07_425723 129 19 marxianus marxianus NN 10_1101-2021_01_07_425723 129 20 for for IN 10_1101-2021_01_07_425723 129 21 contrasts contrast NNS 10_1101-2021_01_07_425723 129 22 21 21 CD 10_1101-2021_01_07_425723 129 23 ( ( -LRB- 10_1101-2021_01_07_425723 129 24 O2 o2 NN 10_1101-2021_01_07_425723 129 25 840 840 CD 10_1101-2021_01_07_425723 129 26 TE te NN 10_1101-2021_01_07_425723 129 27 |O |o CD 10_1101-2021_01_07_425723 129 28 21·104 21·104 CD 10_1101-2021_01_07_425723 129 29 E E NNP 10_1101-2021_01_07_425723 129 30 ) ) -RRB- 10_1101-2021_01_07_425723 129 31 , , , 10_1101-2021_01_07_425723 129 32 31 31 CD 10_1101-2021_01_07_425723 129 33 197 197 CD 10_1101-2021_01_07_425723 129 34 .CC .CC : 10_1101-2021_01_07_425723 129 35 - - HYPH 10_1101-2021_01_07_425723 129 36 BY by IN 10_1101-2021_01_07_425723 129 37 - - HYPH 10_1101-2021_01_07_425723 129 38 NC NC NNP 10_1101-2021_01_07_425723 129 39 - - HYPH 10_1101-2021_01_07_425723 129 40 ND ND NNP 10_1101-2021_01_07_425723 129 41 4.0 4.0 CD 10_1101-2021_01_07_425723 129 42 International International NNP 10_1101-2021_01_07_425723 129 43 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 129 44 under under IN 10_1101-2021_01_07_425723 129 45 a a DT 10_1101-2021_01_07_425723 129 46 ( ( -LRB- 10_1101-2021_01_07_425723 129 47 which which WDT 10_1101-2021_01_07_425723 129 48 was be VBD 10_1101-2021_01_07_425723 129 49 not not RB 10_1101-2021_01_07_425723 129 50 certified certify VBN 10_1101-2021_01_07_425723 129 51 by by IN 10_1101-2021_01_07_425723 129 52 peer peer NN 10_1101-2021_01_07_425723 129 53 review review NN 10_1101-2021_01_07_425723 129 54 ) ) -RRB- 10_1101-2021_01_07_425723 129 55 is be VBZ 10_1101-2021_01_07_425723 129 56 the the DT 10_1101-2021_01_07_425723 129 57 author author NN 10_1101-2021_01_07_425723 129 58 / / SYM 10_1101-2021_01_07_425723 129 59 funder funder NN 10_1101-2021_01_07_425723 129 60 , , , 10_1101-2021_01_07_425723 129 61 who who WP 10_1101-2021_01_07_425723 129 62 has have VBZ 10_1101-2021_01_07_425723 129 63 granted grant VBN 10_1101-2021_01_07_425723 129 64 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 129 65 a a DT 10_1101-2021_01_07_425723 129 66 license license NN 10_1101-2021_01_07_425723 129 67 to to TO 10_1101-2021_01_07_425723 129 68 display display VB 10_1101-2021_01_07_425723 129 69 the the DT 10_1101-2021_01_07_425723 129 70 preprint preprint NN 10_1101-2021_01_07_425723 129 71 in in IN 10_1101-2021_01_07_425723 129 72 perpetuity perpetuity NN 10_1101-2021_01_07_425723 129 73 . . . 10_1101-2021_01_07_425723 130 1 It -PRON- PRP 10_1101-2021_01_07_425723 130 2 is be VBZ 10_1101-2021_01_07_425723 130 3 made make VBN 10_1101-2021_01_07_425723 130 4 The the DT 10_1101-2021_01_07_425723 130 5 copyright copyright NN 10_1101-2021_01_07_425723 130 6 holder holder NN 10_1101-2021_01_07_425723 130 7 for for IN 10_1101-2021_01_07_425723 130 8 this this DT 10_1101-2021_01_07_425723 130 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 130 10 version version NN 10_1101-2021_01_07_425723 130 11 posted post VBD 10_1101-2021_01_07_425723 130 12 January January NNP 10_1101-2021_01_07_425723 130 13 8 8 CD 10_1101-2021_01_07_425723 130 14 , , , 10_1101-2021_01_07_425723 130 15 2021 2021 CD 10_1101-2021_01_07_425723 130 16 . . . 10_1101-2021_01_07_425723 130 17 ; ; : 10_1101-2021_01_07_425723 130 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 130 19 : : : 10_1101-2021_01_07_425723 130 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 130 21 preprint preprint NN 10_1101-2021_01_07_425723 130 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 130 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 130 24 13 13 CD 10_1101-2021_01_07_425723 130 25 ( ( -LRB- 10_1101-2021_01_07_425723 130 26 O2 o2 NN 10_1101-2021_01_07_425723 130 27 0.5 0.5 CD 10_1101-2021_01_07_425723 130 28 TE TE NNP 10_1101-2021_01_07_425723 130 29 | | NNP 10_1101-2021_01_07_425723 130 30 O o NN 10_1101-2021_01_07_425723 130 31 21·104 21·104 CD 10_1101-2021_01_07_425723 130 32 E e NN 10_1101-2021_01_07_425723 130 33 ) ) -RRB- 10_1101-2021_01_07_425723 130 34 , , , 10_1101-2021_01_07_425723 130 35 32 32 CD 10_1101-2021_01_07_425723 130 36 ( ( -LRB- 10_1101-2021_01_07_425723 130 37 O2 o2 NN 10_1101-2021_01_07_425723 130 38 0.5 0.5 CD 10_1101-2021_01_07_425723 130 39 TE TE NNP 10_1101-2021_01_07_425723 130 40 | | CD 10_1101-2021_01_07_425723 130 41 O2 O2 VBN 10_1101-2021_01_07_425723 130 42 840 840 CD 10_1101-2021_01_07_425723 130 43 TE TE NNP 10_1101-2021_01_07_425723 130 44 ) ) -RRB- 10_1101-2021_01_07_425723 130 45 , , , 10_1101-2021_01_07_425723 130 46 43 43 CD 10_1101-2021_01_07_425723 130 47 ( ( -LRB- 10_1101-2021_01_07_425723 130 48 O2 o2 NN 10_1101-2021_01_07_425723 130 49 0.5 0.5 CD 10_1101-2021_01_07_425723 130 50 T T NNP 10_1101-2021_01_07_425723 130 51 | | CD 10_1101-2021_01_07_425723 130 52 O2 O2 VBN 10_1101-2021_01_07_425723 130 53 0.5 0.5 CD 10_1101-2021_01_07_425723 130 54 TE TE NNP 10_1101-2021_01_07_425723 130 55 ) ) -RRB- 10_1101-2021_01_07_425723 130 56 , , , 10_1101-2021_01_07_425723 130 57 54 54 CD 10_1101-2021_01_07_425723 130 58 ( ( -LRB- 10_1101-2021_01_07_425723 130 59 O2 o2 NN 10_1101-2021_01_07_425723 130 60 0.5 0.5 CD 10_1101-2021_01_07_425723 130 61 | | CD 10_1101-2021_01_07_425723 130 62 O2 O2 VBN 10_1101-2021_01_07_425723 130 63 0.5 0.5 CD 10_1101-2021_01_07_425723 130 64 T t NN 10_1101-2021_01_07_425723 130 65 ) ) -RRB- 10_1101-2021_01_07_425723 130 66 . . . 10_1101-2021_01_07_425723 131 1 198 198 CD 10_1101-2021_01_07_425723 131 2 Lumped Lumped NNP 10_1101-2021_01_07_425723 131 3 biochemical biochemical JJ 10_1101-2021_01_07_425723 131 4 reactions reaction NNS 10_1101-2021_01_07_425723 131 5 are be VBP 10_1101-2021_01_07_425723 131 6 represented represent VBN 10_1101-2021_01_07_425723 131 7 by by IN 10_1101-2021_01_07_425723 131 8 arrows arrow NNS 10_1101-2021_01_07_425723 131 9 . . . 10_1101-2021_01_07_425723 132 1 Colors color NNS 10_1101-2021_01_07_425723 132 2 indicate indicate VBP 10_1101-2021_01_07_425723 132 3 up- up- CD 10_1101-2021_01_07_425723 132 4 ( ( -LRB- 10_1101-2021_01_07_425723 132 5 blue blue JJ 10_1101-2021_01_07_425723 132 6 ) ) -RRB- 10_1101-2021_01_07_425723 132 7 or or CC 10_1101-2021_01_07_425723 132 8 down down IN 10_1101-2021_01_07_425723 132 9 - - HYPH 10_1101-2021_01_07_425723 132 10 regulation regulation NN 10_1101-2021_01_07_425723 132 11 199 199 CD 10_1101-2021_01_07_425723 132 12 ( ( -LRB- 10_1101-2021_01_07_425723 132 13 brown brown NNP 10_1101-2021_01_07_425723 132 14 ) ) -RRB- 10_1101-2021_01_07_425723 132 15 with with IN 10_1101-2021_01_07_425723 132 16 color color NN 10_1101-2021_01_07_425723 132 17 intensity intensity NN 10_1101-2021_01_07_425723 132 18 indicating indicate VBG 10_1101-2021_01_07_425723 132 19 the the DT 10_1101-2021_01_07_425723 132 20 log log NN 10_1101-2021_01_07_425723 132 21 2 2 CD 10_1101-2021_01_07_425723 132 22 fold fold JJ 10_1101-2021_01_07_425723 132 23 change change NN 10_1101-2021_01_07_425723 132 24 with with IN 10_1101-2021_01_07_425723 132 25 color color NN 10_1101-2021_01_07_425723 132 26 range range NN 10_1101-2021_01_07_425723 132 27 capped cap VBN 10_1101-2021_01_07_425723 132 28 to to IN 10_1101-2021_01_07_425723 132 29 a a DT 10_1101-2021_01_07_425723 132 30 maximum maximum NN 10_1101-2021_01_07_425723 132 31 of of IN 10_1101-2021_01_07_425723 132 32 4 4 CD 10_1101-2021_01_07_425723 132 33 . . . 10_1101-2021_01_07_425723 133 1 200 200 CD 10_1101-2021_01_07_425723 133 2 Reactions reaction NNS 10_1101-2021_01_07_425723 133 3 are be VBP 10_1101-2021_01_07_425723 133 4 annotated annotate VBN 10_1101-2021_01_07_425723 133 5 with with IN 10_1101-2021_01_07_425723 133 6 corresponding correspond VBG 10_1101-2021_01_07_425723 133 7 gene gene NN 10_1101-2021_01_07_425723 133 8 , , , 10_1101-2021_01_07_425723 133 9 K. K. NNP 10_1101-2021_01_07_425723 133 10 marxianus marxianus NN 10_1101-2021_01_07_425723 133 11 genes gene NNS 10_1101-2021_01_07_425723 133 12 are be VBP 10_1101-2021_01_07_425723 133 13 indicated indicate VBN 10_1101-2021_01_07_425723 133 14 with with IN 10_1101-2021_01_07_425723 133 15 the the DT 10_1101-2021_01_07_425723 133 16 name name NN 10_1101-2021_01_07_425723 133 17 of of IN 10_1101-2021_01_07_425723 133 18 201 201 CD 10_1101-2021_01_07_425723 133 19 the the DT 10_1101-2021_01_07_425723 133 20 S. S. NNP 10_1101-2021_01_07_425723 133 21 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 133 22 orthologs ortholog VBZ 10_1101-2021_01_07_425723 133 23 . . . 10_1101-2021_01_07_425723 134 1 Ergosterol Ergosterol NNP 10_1101-2021_01_07_425723 134 2 uptake uptake NN 10_1101-2021_01_07_425723 134 3 by by IN 10_1101-2021_01_07_425723 134 4 S. S. NNP 10_1101-2021_01_07_425723 134 5 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 134 6 requires require VBZ 10_1101-2021_01_07_425723 134 7 additional additional JJ 10_1101-2021_01_07_425723 134 8 factors factor NNS 10_1101-2021_01_07_425723 134 9 beyond beyond IN 10_1101-2021_01_07_425723 134 10 the the DT 10_1101-2021_01_07_425723 134 11 202 202 CD 10_1101-2021_01_07_425723 134 12 membrane membrane NN 10_1101-2021_01_07_425723 134 13 transporters transporter NNS 10_1101-2021_01_07_425723 134 14 Aus1 Aus1 NNP 10_1101-2021_01_07_425723 134 15 and and CC 10_1101-2021_01_07_425723 134 16 Pdr1142 Pdr1142 NNP 10_1101-2021_01_07_425723 134 17 . . . 10_1101-2021_01_07_425723 135 1 No no DT 10_1101-2021_01_07_425723 135 2 orthologs ortholog NNS 10_1101-2021_01_07_425723 135 3 of of IN 10_1101-2021_01_07_425723 135 4 the the DT 10_1101-2021_01_07_425723 135 5 sterol sterol NN 10_1101-2021_01_07_425723 135 6 - - HYPH 10_1101-2021_01_07_425723 135 7 transporters transporter NNS 10_1101-2021_01_07_425723 135 8 or or CC 10_1101-2021_01_07_425723 135 9 Hmg2 Hmg2 NNP 10_1101-2021_01_07_425723 135 10 were be VBD 10_1101-2021_01_07_425723 135 11 203 203 CD 10_1101-2021_01_07_425723 135 12 identified identify VBN 10_1101-2021_01_07_425723 135 13 for for IN 10_1101-2021_01_07_425723 135 14 K. K. NNP 10_1101-2021_01_07_425723 135 15 marxianus marxianus NN 10_1101-2021_01_07_425723 135 16 and and CC 10_1101-2021_01_07_425723 135 17 low low JJ 10_1101-2021_01_07_425723 135 18 read read VBN 10_1101-2021_01_07_425723 135 19 counts count NNS 10_1101-2021_01_07_425723 135 20 for for IN 10_1101-2021_01_07_425723 135 21 Erg3 Erg3 NNP 10_1101-2021_01_07_425723 135 22 , , , 10_1101-2021_01_07_425723 135 23 Erg9 Erg9 NNP 10_1101-2021_01_07_425723 135 24 and and CC 10_1101-2021_01_07_425723 135 25 Erg20 Erg20 NNP 10_1101-2021_01_07_425723 135 26 precluded preclude VBD 10_1101-2021_01_07_425723 135 27 differential differential NN 10_1101-2021_01_07_425723 135 28 gene gene NN 10_1101-2021_01_07_425723 135 29 204 204 CD 10_1101-2021_01_07_425723 135 30 expression expression NN 10_1101-2021_01_07_425723 135 31 analysis analysis NN 10_1101-2021_01_07_425723 135 32 across across IN 10_1101-2021_01_07_425723 135 33 all all DT 10_1101-2021_01_07_425723 135 34 conditions condition NNS 10_1101-2021_01_07_425723 135 35 ( ( -LRB- 10_1101-2021_01_07_425723 135 36 dark dark JJ 10_1101-2021_01_07_425723 135 37 grey grey NN 10_1101-2021_01_07_425723 135 38 ) ) -RRB- 10_1101-2021_01_07_425723 135 39 . . . 10_1101-2021_01_07_425723 136 1 Enzyme Enzyme NNS 10_1101-2021_01_07_425723 136 2 abbreviations abbreviation NNS 10_1101-2021_01_07_425723 136 3 : : : 10_1101-2021_01_07_425723 136 4 Erg10 erg10 NN 10_1101-2021_01_07_425723 136 5 acetyl acetyl NN 10_1101-2021_01_07_425723 136 6 - - HYPH 10_1101-2021_01_07_425723 136 7 CoA CoA NNP 10_1101-2021_01_07_425723 136 8 205 205 CD 10_1101-2021_01_07_425723 136 9 acetyltransferase acetyltransferase NN 10_1101-2021_01_07_425723 136 10 , , , 10_1101-2021_01_07_425723 136 11 Erg13 Erg13 NNP 10_1101-2021_01_07_425723 136 12 3-hydroxy-3-methylglutaryl 3-hydroxy-3-methylglutaryl NNP 10_1101-2021_01_07_425723 136 13 - - HYPH 10_1101-2021_01_07_425723 136 14 CoA CoA NNP 10_1101-2021_01_07_425723 136 15 ( ( -LRB- 10_1101-2021_01_07_425723 136 16 HMG HMG NNP 10_1101-2021_01_07_425723 136 17 - - HYPH 10_1101-2021_01_07_425723 136 18 CoA CoA NNP 10_1101-2021_01_07_425723 136 19 ) ) -RRB- 10_1101-2021_01_07_425723 136 20 synthase synthase NN 10_1101-2021_01_07_425723 136 21 , , , 10_1101-2021_01_07_425723 136 22 Hmg1 Hmg1 NNP 10_1101-2021_01_07_425723 136 23 / / SYM 10_1101-2021_01_07_425723 136 24 Hmg2 Hmg2 NNP 10_1101-2021_01_07_425723 136 25 HMG HMG NNP 10_1101-2021_01_07_425723 136 26 - - HYPH 10_1101-2021_01_07_425723 136 27 CoA CoA NNP 10_1101-2021_01_07_425723 136 28 206 206 CD 10_1101-2021_01_07_425723 136 29 reductase reductase NN 10_1101-2021_01_07_425723 136 30 , , , 10_1101-2021_01_07_425723 136 31 Erg12 Erg12 NNP 10_1101-2021_01_07_425723 136 32 mevalonate mevalonate NN 10_1101-2021_01_07_425723 136 33 kinase kinase NN 10_1101-2021_01_07_425723 136 34 , , , 10_1101-2021_01_07_425723 136 35 Erg8 erg8 RB 10_1101-2021_01_07_425723 136 36 phosphomevalonate phosphomevalonate NN 10_1101-2021_01_07_425723 136 37 kinase kinase NN 10_1101-2021_01_07_425723 136 38 , , , 10_1101-2021_01_07_425723 136 39 Mvd1 Mvd1 NNP 10_1101-2021_01_07_425723 136 40 mevalonate mevalonate VB 10_1101-2021_01_07_425723 136 41 207 207 CD 10_1101-2021_01_07_425723 136 42 pyrophosphate pyrophosphate NN 10_1101-2021_01_07_425723 136 43 decarboxylase decarboxylase NN 10_1101-2021_01_07_425723 136 44 , , , 10_1101-2021_01_07_425723 136 45 Idi1 idi1 NN 10_1101-2021_01_07_425723 136 46 isopentenyl isopentenyl RB 10_1101-2021_01_07_425723 136 47 diphosphate diphosphate VB 10_1101-2021_01_07_425723 136 48 : : : 10_1101-2021_01_07_425723 136 49 dimethylallyl dimethylallyl JJ 10_1101-2021_01_07_425723 136 50 diphosphate diphosphate NNP 10_1101-2021_01_07_425723 136 51 ( ( -LRB- 10_1101-2021_01_07_425723 136 52 IPP IPP NNP 10_1101-2021_01_07_425723 136 53 ) ) -RRB- 10_1101-2021_01_07_425723 136 54 isomerase isomerase NN 10_1101-2021_01_07_425723 136 55 , , , 10_1101-2021_01_07_425723 136 56 208 208 CD 10_1101-2021_01_07_425723 136 57 Erg20 Erg20 NNP 10_1101-2021_01_07_425723 136 58 farnesyl farnesyl JJ 10_1101-2021_01_07_425723 136 59 pyrophosphate pyrophosphate NN 10_1101-2021_01_07_425723 136 60 synthetase synthetase NN 10_1101-2021_01_07_425723 136 61 , , , 10_1101-2021_01_07_425723 136 62 Erg9 erg9 JJ 10_1101-2021_01_07_425723 136 63 farnesyl farnesyl NN 10_1101-2021_01_07_425723 136 64 - - HYPH 10_1101-2021_01_07_425723 136 65 diphosphate diphosphate NN 10_1101-2021_01_07_425723 136 66 transferase transferase NN 10_1101-2021_01_07_425723 136 67 ( ( -LRB- 10_1101-2021_01_07_425723 136 68 squalene squalene NNP 10_1101-2021_01_07_425723 136 69 synthase synthase NNP 10_1101-2021_01_07_425723 136 70 ) ) -RRB- 10_1101-2021_01_07_425723 136 71 , , , 10_1101-2021_01_07_425723 136 72 209 209 CD 10_1101-2021_01_07_425723 136 73 Erg7 erg7 JJ 10_1101-2021_01_07_425723 136 74 lanosterol lanosterol NN 10_1101-2021_01_07_425723 136 75 synthase synthase NN 10_1101-2021_01_07_425723 136 76 , , , 10_1101-2021_01_07_425723 136 77 Erg11 Erg11 NNP 10_1101-2021_01_07_425723 136 78 lanosterol lanosterol NN 10_1101-2021_01_07_425723 136 79 14α 14α NNS 10_1101-2021_01_07_425723 136 80 - - : 10_1101-2021_01_07_425723 136 81 demethylase demethylase NN 10_1101-2021_01_07_425723 136 82 , , , 10_1101-2021_01_07_425723 136 83 Cyb5 Cyb5 NNP 10_1101-2021_01_07_425723 136 84 cytochrome cytochrome VB 10_1101-2021_01_07_425723 136 85 b5 b5 NN 10_1101-2021_01_07_425723 136 86 ( ( -LRB- 10_1101-2021_01_07_425723 136 87 electron electron NN 10_1101-2021_01_07_425723 136 88 donor donor NN 10_1101-2021_01_07_425723 136 89 for for IN 10_1101-2021_01_07_425723 136 90 210 210 CD 10_1101-2021_01_07_425723 136 91 sterol sterol NN 10_1101-2021_01_07_425723 136 92 C5 C5 NNP 10_1101-2021_01_07_425723 136 93 - - HYPH 10_1101-2021_01_07_425723 136 94 6 6 CD 10_1101-2021_01_07_425723 136 95 desaturation desaturation NN 10_1101-2021_01_07_425723 136 96 ) ) -RRB- 10_1101-2021_01_07_425723 136 97 , , , 10_1101-2021_01_07_425723 136 98 Ncp1 Ncp1 NNP 10_1101-2021_01_07_425723 136 99 NADP NADP NNP 10_1101-2021_01_07_425723 136 100 - - HYPH 10_1101-2021_01_07_425723 136 101 cytochrome cytochrome NNP 10_1101-2021_01_07_425723 136 102 P450 p450 NN 10_1101-2021_01_07_425723 136 103 reductase reductase NN 10_1101-2021_01_07_425723 136 104 , , , 10_1101-2021_01_07_425723 136 105 Erg24 Erg24 NNP 10_1101-2021_01_07_425723 136 106 C-14 c-14 CD 10_1101-2021_01_07_425723 136 107 sterol sterol JJ 10_1101-2021_01_07_425723 136 108 reductase reductase NN 10_1101-2021_01_07_425723 136 109 , , , 10_1101-2021_01_07_425723 136 110 Erg25 Erg25 NNP 10_1101-2021_01_07_425723 136 111 C-211 C-211 NNP 10_1101-2021_01_07_425723 136 112 4 4 CD 10_1101-2021_01_07_425723 136 113 methyl methyl NN 10_1101-2021_01_07_425723 136 114 sterol sterol NN 10_1101-2021_01_07_425723 136 115 oxidase oxidase NN 10_1101-2021_01_07_425723 136 116 , , , 10_1101-2021_01_07_425723 136 117 Erg26 Erg26 NNP 10_1101-2021_01_07_425723 136 118 C-3 C-3 NNP 10_1101-2021_01_07_425723 136 119 sterol sterol NN 10_1101-2021_01_07_425723 136 120 dehydrogenase dehydrogenase NN 10_1101-2021_01_07_425723 136 121 , , , 10_1101-2021_01_07_425723 136 122 Erg27 Erg27 NNP 10_1101-2021_01_07_425723 136 123 3-keto 3-keto NNP 10_1101-2021_01_07_425723 136 124 - - HYPH 10_1101-2021_01_07_425723 136 125 sterol sterol NN 10_1101-2021_01_07_425723 136 126 reductase reductase NN 10_1101-2021_01_07_425723 136 127 , , , 10_1101-2021_01_07_425723 136 128 Erg28 Erg28 NNP 10_1101-2021_01_07_425723 136 129 212 212 CD 10_1101-2021_01_07_425723 136 130 endoplasmic endoplasmic JJ 10_1101-2021_01_07_425723 136 131 reticulum reticulum NN 10_1101-2021_01_07_425723 136 132 membrane membrane NN 10_1101-2021_01_07_425723 136 133 protein protein NN 10_1101-2021_01_07_425723 136 134 ( ( -LRB- 10_1101-2021_01_07_425723 136 135 may may MD 10_1101-2021_01_07_425723 136 136 facilitate facilitate VB 10_1101-2021_01_07_425723 136 137 protein protein NN 10_1101-2021_01_07_425723 136 138 - - HYPH 10_1101-2021_01_07_425723 136 139 protein protein NN 10_1101-2021_01_07_425723 136 140 interactions interaction NNS 10_1101-2021_01_07_425723 136 141 between between IN 10_1101-2021_01_07_425723 136 142 Erg26 Erg26 NNP 10_1101-2021_01_07_425723 136 143 213 213 CD 10_1101-2021_01_07_425723 136 144 and and CC 10_1101-2021_01_07_425723 136 145 Erg27 Erg27 NNP 10_1101-2021_01_07_425723 136 146 , , , 10_1101-2021_01_07_425723 136 147 or or CC 10_1101-2021_01_07_425723 136 148 tether tether RB 10_1101-2021_01_07_425723 136 149 these these DT 10_1101-2021_01_07_425723 136 150 to to IN 10_1101-2021_01_07_425723 136 151 the the DT 10_1101-2021_01_07_425723 136 152 ER ER NNP 10_1101-2021_01_07_425723 136 153 ) ) -RRB- 10_1101-2021_01_07_425723 136 154 , , , 10_1101-2021_01_07_425723 136 155 Erg6 Erg6 NNP 10_1101-2021_01_07_425723 136 156 Δ24-sterol Δ24-sterol NNP 10_1101-2021_01_07_425723 136 157 C C NNP 10_1101-2021_01_07_425723 136 158 - - HYPH 10_1101-2021_01_07_425723 136 159 methyltransferase methyltransferase NNP 10_1101-2021_01_07_425723 136 160 , , , 10_1101-2021_01_07_425723 136 161 Erg2 Erg2 NNP 10_1101-2021_01_07_425723 136 162 Δ24-sterol Δ24-sterol NNP 10_1101-2021_01_07_425723 136 163 C-214 C-214 NNP 10_1101-2021_01_07_425723 136 164 methyltransferase methyltransferase NN 10_1101-2021_01_07_425723 136 165 , , , 10_1101-2021_01_07_425723 136 166 Erg3 erg3 JJ 10_1101-2021_01_07_425723 136 167 C-5 C-5 NNP 10_1101-2021_01_07_425723 136 168 sterol sterol NN 10_1101-2021_01_07_425723 136 169 desaturase desaturase NN 10_1101-2021_01_07_425723 136 170 , , , 10_1101-2021_01_07_425723 136 171 Erg5 Erg5 NNP 10_1101-2021_01_07_425723 136 172 C-22 c-22 CD 10_1101-2021_01_07_425723 136 173 sterol sterol NN 10_1101-2021_01_07_425723 136 174 desaturase desaturase NN 10_1101-2021_01_07_425723 136 175 , , , 10_1101-2021_01_07_425723 136 176 Erg4 erg4 VB 10_1101-2021_01_07_425723 136 177 C24/28 C24/28 NNP 10_1101-2021_01_07_425723 136 178 sterol sterol NN 10_1101-2021_01_07_425723 136 179 215 215 CD 10_1101-2021_01_07_425723 136 180 reductase reductase NN 10_1101-2021_01_07_425723 136 181 , , , 10_1101-2021_01_07_425723 136 182 Aus1 Aus1 NNP 10_1101-2021_01_07_425723 136 183 / / SYM 10_1101-2021_01_07_425723 136 184 Pdr11 Pdr11 NNP 10_1101-2021_01_07_425723 136 185 plasma plasma NN 10_1101-2021_01_07_425723 136 186 - - HYPH 10_1101-2021_01_07_425723 136 187 membrane membrane NNP 10_1101-2021_01_07_425723 136 188 sterol sterol NN 10_1101-2021_01_07_425723 136 189 transporter transporter NN 10_1101-2021_01_07_425723 136 190 . . . 10_1101-2021_01_07_425723 137 1 216 216 CD 10_1101-2021_01_07_425723 137 2 .CC .CC : 10_1101-2021_01_07_425723 137 3 - - : 10_1101-2021_01_07_425723 137 4 BY by IN 10_1101-2021_01_07_425723 137 5 - - HYPH 10_1101-2021_01_07_425723 137 6 NC NC NNP 10_1101-2021_01_07_425723 137 7 - - HYPH 10_1101-2021_01_07_425723 137 8 ND ND NNP 10_1101-2021_01_07_425723 137 9 4.0 4.0 CD 10_1101-2021_01_07_425723 137 10 International International NNP 10_1101-2021_01_07_425723 137 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 137 12 under under IN 10_1101-2021_01_07_425723 137 13 a a DT 10_1101-2021_01_07_425723 137 14 ( ( -LRB- 10_1101-2021_01_07_425723 137 15 which which WDT 10_1101-2021_01_07_425723 137 16 was be VBD 10_1101-2021_01_07_425723 137 17 not not RB 10_1101-2021_01_07_425723 137 18 certified certify VBN 10_1101-2021_01_07_425723 137 19 by by IN 10_1101-2021_01_07_425723 137 20 peer peer NN 10_1101-2021_01_07_425723 137 21 review review NN 10_1101-2021_01_07_425723 137 22 ) ) -RRB- 10_1101-2021_01_07_425723 137 23 is be VBZ 10_1101-2021_01_07_425723 137 24 the the DT 10_1101-2021_01_07_425723 137 25 author author NN 10_1101-2021_01_07_425723 137 26 / / SYM 10_1101-2021_01_07_425723 137 27 funder funder NN 10_1101-2021_01_07_425723 137 28 , , , 10_1101-2021_01_07_425723 137 29 who who WP 10_1101-2021_01_07_425723 137 30 has have VBZ 10_1101-2021_01_07_425723 137 31 granted grant VBN 10_1101-2021_01_07_425723 137 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 137 33 a a DT 10_1101-2021_01_07_425723 137 34 license license NN 10_1101-2021_01_07_425723 137 35 to to TO 10_1101-2021_01_07_425723 137 36 display display VB 10_1101-2021_01_07_425723 137 37 the the DT 10_1101-2021_01_07_425723 137 38 preprint preprint NN 10_1101-2021_01_07_425723 137 39 in in IN 10_1101-2021_01_07_425723 137 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 137 41 . . . 10_1101-2021_01_07_425723 138 1 It -PRON- PRP 10_1101-2021_01_07_425723 138 2 is be VBZ 10_1101-2021_01_07_425723 138 3 made make VBN 10_1101-2021_01_07_425723 138 4 The the DT 10_1101-2021_01_07_425723 138 5 copyright copyright NN 10_1101-2021_01_07_425723 138 6 holder holder NN 10_1101-2021_01_07_425723 138 7 for for IN 10_1101-2021_01_07_425723 138 8 this this DT 10_1101-2021_01_07_425723 138 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 138 10 version version NN 10_1101-2021_01_07_425723 138 11 posted post VBD 10_1101-2021_01_07_425723 138 12 January January NNP 10_1101-2021_01_07_425723 138 13 8 8 CD 10_1101-2021_01_07_425723 138 14 , , , 10_1101-2021_01_07_425723 138 15 2021 2021 CD 10_1101-2021_01_07_425723 138 16 . . . 10_1101-2021_01_07_425723 138 17 ; ; : 10_1101-2021_01_07_425723 138 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 138 19 : : : 10_1101-2021_01_07_425723 138 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 138 21 preprint preprint NN 10_1101-2021_01_07_425723 138 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 138 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ RB 10_1101-2021_01_07_425723 138 24 14 14 CD 10_1101-2021_01_07_425723 138 25 Absence absence NN 10_1101-2021_01_07_425723 138 26 of of IN 10_1101-2021_01_07_425723 138 27 sterol sterol NN 10_1101-2021_01_07_425723 138 28 import import NN 10_1101-2021_01_07_425723 138 29 in in IN 10_1101-2021_01_07_425723 138 30 K. K. NNP 10_1101-2021_01_07_425723 138 31 marxianus marxianus NNP 10_1101-2021_01_07_425723 138 32 217 217 CD 10_1101-2021_01_07_425723 138 33 To to TO 10_1101-2021_01_07_425723 138 34 test test VB 10_1101-2021_01_07_425723 138 35 the the DT 10_1101-2021_01_07_425723 138 36 hypothesis hypothesis NN 10_1101-2021_01_07_425723 138 37 that that WDT 10_1101-2021_01_07_425723 138 38 K. K. NNP 10_1101-2021_01_07_425723 138 39 marxianus marxianus NN 10_1101-2021_01_07_425723 138 40 lacks lack VBZ 10_1101-2021_01_07_425723 138 41 a a DT 10_1101-2021_01_07_425723 138 42 functional functional JJ 10_1101-2021_01_07_425723 138 43 sterol sterol JJ 10_1101-2021_01_07_425723 138 44 - - HYPH 10_1101-2021_01_07_425723 138 45 uptake uptake NN 10_1101-2021_01_07_425723 138 46 mechanism mechanism NN 10_1101-2021_01_07_425723 138 47 , , , 10_1101-2021_01_07_425723 138 48 uptake uptake JJ 10_1101-2021_01_07_425723 138 49 of of IN 10_1101-2021_01_07_425723 138 50 218 218 CD 10_1101-2021_01_07_425723 138 51 fluorescent fluorescent NN 10_1101-2021_01_07_425723 138 52 sterol sterol NN 10_1101-2021_01_07_425723 138 53 derivative derivative JJ 10_1101-2021_01_07_425723 138 54 25-NBD 25-nbd CD 10_1101-2021_01_07_425723 138 55 - - HYPH 10_1101-2021_01_07_425723 138 56 cholesterol cholesterol NN 10_1101-2021_01_07_425723 138 57 ( ( -LRB- 10_1101-2021_01_07_425723 138 58 NBDC NBDC NNP 10_1101-2021_01_07_425723 138 59 ) ) -RRB- 10_1101-2021_01_07_425723 138 60 was be VBD 10_1101-2021_01_07_425723 138 61 measured measure VBN 10_1101-2021_01_07_425723 138 62 by by IN 10_1101-2021_01_07_425723 138 63 flow flow NN 10_1101-2021_01_07_425723 138 64 cytometry43 cytometry43 NNS 10_1101-2021_01_07_425723 138 65 . . . 10_1101-2021_01_07_425723 139 1 Since since IN 10_1101-2021_01_07_425723 139 2 S. S. NNP 10_1101-2021_01_07_425723 139 3 219 219 CD 10_1101-2021_01_07_425723 139 4 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 139 5 sterol sterol NN 10_1101-2021_01_07_425723 139 6 transporters transporter NNS 10_1101-2021_01_07_425723 139 7 are be VBP 10_1101-2021_01_07_425723 139 8 not not RB 10_1101-2021_01_07_425723 139 9 expressed express VBN 10_1101-2021_01_07_425723 139 10 in in IN 10_1101-2021_01_07_425723 139 11 aerobic aerobic JJ 10_1101-2021_01_07_425723 139 12 conditions20 conditions20 NN 10_1101-2021_01_07_425723 139 13 and and CC 10_1101-2021_01_07_425723 139 14 to to TO 10_1101-2021_01_07_425723 139 15 avoid avoid VB 10_1101-2021_01_07_425723 139 16 interference interference NN 10_1101-2021_01_07_425723 139 17 of of IN 10_1101-2021_01_07_425723 139 18 220 220 CD 10_1101-2021_01_07_425723 139 19 sterol sterol JJ 10_1101-2021_01_07_425723 139 20 synthesis synthesis NN 10_1101-2021_01_07_425723 139 21 , , , 10_1101-2021_01_07_425723 139 22 NBDC NBDC NNP 10_1101-2021_01_07_425723 139 23 uptake uptake NN 10_1101-2021_01_07_425723 139 24 was be VBD 10_1101-2021_01_07_425723 139 25 analysed analyse VBN 10_1101-2021_01_07_425723 139 26 in in IN 10_1101-2021_01_07_425723 139 27 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 139 28 cell cell NN 10_1101-2021_01_07_425723 139 29 suspensions suspension NNS 10_1101-2021_01_07_425723 139 30 ( ( -LRB- 10_1101-2021_01_07_425723 139 31 Fig fig NN 10_1101-2021_01_07_425723 139 32 . . . 10_1101-2021_01_07_425723 140 1 4a 4a LS 10_1101-2021_01_07_425723 140 2 ) ) -RRB- 10_1101-2021_01_07_425723 140 3 . . . 10_1101-2021_01_07_425723 141 1 Four four CD 10_1101-2021_01_07_425723 141 2 hours hour NNS 10_1101-2021_01_07_425723 141 3 after after IN 10_1101-2021_01_07_425723 141 4 221 221 CD 10_1101-2021_01_07_425723 141 5 NBDC NBDC NNP 10_1101-2021_01_07_425723 141 6 addition addition NN 10_1101-2021_01_07_425723 141 7 to to IN 10_1101-2021_01_07_425723 141 8 cell cell NN 10_1101-2021_01_07_425723 141 9 suspensions suspension NNS 10_1101-2021_01_07_425723 141 10 of of IN 10_1101-2021_01_07_425723 141 11 the the DT 10_1101-2021_01_07_425723 141 12 reference reference NN 10_1101-2021_01_07_425723 141 13 strain strain NN 10_1101-2021_01_07_425723 141 14 S. S. NNP 10_1101-2021_01_07_425723 141 15 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 141 16 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 141 17 , , , 10_1101-2021_01_07_425723 141 18 median median JJ 10_1101-2021_01_07_425723 141 19 single single JJ 10_1101-2021_01_07_425723 141 20 - - HYPH 10_1101-2021_01_07_425723 141 21 cell cell NN 10_1101-2021_01_07_425723 141 22 222 222 CD 10_1101-2021_01_07_425723 141 23 fluorescence fluorescence NN 10_1101-2021_01_07_425723 141 24 increased increase VBN 10_1101-2021_01_07_425723 141 25 by by IN 10_1101-2021_01_07_425723 141 26 66-fold 66-fold CD 10_1101-2021_01_07_425723 141 27 ( ( -LRB- 10_1101-2021_01_07_425723 141 28 Fig fig NN 10_1101-2021_01_07_425723 141 29 . . . 10_1101-2021_01_07_425723 142 1 4bc 4bc NNP 10_1101-2021_01_07_425723 142 2 ) ) -RRB- 10_1101-2021_01_07_425723 142 3 . . . 10_1101-2021_01_07_425723 143 1 In in IN 10_1101-2021_01_07_425723 143 2 contrast contrast NN 10_1101-2021_01_07_425723 143 3 , , , 10_1101-2021_01_07_425723 143 4 the the DT 10_1101-2021_01_07_425723 143 5 congenic congenic JJ 10_1101-2021_01_07_425723 143 6 sterol sterol JJ 10_1101-2021_01_07_425723 143 7 - - HYPH 10_1101-2021_01_07_425723 143 8 transporter transporter NN 10_1101-2021_01_07_425723 143 9 - - HYPH 10_1101-2021_01_07_425723 143 10 deficient deficient JJ 10_1101-2021_01_07_425723 143 11 strain strain NN 10_1101-2021_01_07_425723 143 12 223 223 CD 10_1101-2021_01_07_425723 143 13 IMK809 IMK809 NNP 10_1101-2021_01_07_425723 143 14 ( ( -LRB- 10_1101-2021_01_07_425723 143 15 aus1Δ aus1Δ NNP 10_1101-2021_01_07_425723 143 16 pdr11Δ pdr11Δ NNP 10_1101-2021_01_07_425723 143 17 ) ) -RRB- 10_1101-2021_01_07_425723 143 18 only only RB 10_1101-2021_01_07_425723 143 19 showed show VBD 10_1101-2021_01_07_425723 143 20 a a DT 10_1101-2021_01_07_425723 143 21 6-fold 6-fold CD 10_1101-2021_01_07_425723 143 22 increase increase NN 10_1101-2021_01_07_425723 143 23 of of IN 10_1101-2021_01_07_425723 143 24 fluorescence fluorescence NN 10_1101-2021_01_07_425723 143 25 , , , 10_1101-2021_01_07_425723 143 26 probably probably RB 10_1101-2021_01_07_425723 143 27 reflected reflect VBD 10_1101-2021_01_07_425723 143 28 detergent-224 detergent-224 JJ 10_1101-2021_01_07_425723 143 29 resistant resistant JJ 10_1101-2021_01_07_425723 143 30 binding binding NN 10_1101-2021_01_07_425723 143 31 of of IN 10_1101-2021_01_07_425723 143 32 NBDC NBDC NNP 10_1101-2021_01_07_425723 143 33 to to IN 10_1101-2021_01_07_425723 143 34 S. S. NNP 10_1101-2021_01_07_425723 143 35 cerevisiae cerevisiae NNP 10_1101-2021_01_07_425723 143 36 cell cell NN 10_1101-2021_01_07_425723 143 37 - - HYPH 10_1101-2021_01_07_425723 143 38 wall wall NN 10_1101-2021_01_07_425723 143 39 proteins43,44 proteins43,44 NNP 10_1101-2021_01_07_425723 143 40 . . . 10_1101-2021_01_07_425723 144 1 K. K. NNP 10_1101-2021_01_07_425723 144 2 marxianus marxianus NN 10_1101-2021_01_07_425723 144 3 strains strain VBZ 10_1101-2021_01_07_425723 144 4 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 144 5 and and CC 10_1101-2021_01_07_425723 144 6 225 225 CD 10_1101-2021_01_07_425723 144 7 NBRC1777 NBRC1777 NNPS 10_1101-2021_01_07_425723 144 8 did do VBD 10_1101-2021_01_07_425723 144 9 not not RB 10_1101-2021_01_07_425723 144 10 show show VB 10_1101-2021_01_07_425723 144 11 increased increase VBN 10_1101-2021_01_07_425723 144 12 fluorescence fluorescence NN 10_1101-2021_01_07_425723 144 13 , , , 10_1101-2021_01_07_425723 144 14 neither neither CC 10_1101-2021_01_07_425723 144 15 after after IN 10_1101-2021_01_07_425723 144 16 4 4 CD 10_1101-2021_01_07_425723 144 17 h h NN 10_1101-2021_01_07_425723 144 18 nor nor CC 10_1101-2021_01_07_425723 144 19 after after IN 10_1101-2021_01_07_425723 144 20 23 23 CD 10_1101-2021_01_07_425723 144 21 h h NNS 10_1101-2021_01_07_425723 144 22 of of IN 10_1101-2021_01_07_425723 144 23 incubation incubation NN 10_1101-2021_01_07_425723 144 24 with with IN 10_1101-2021_01_07_425723 144 25 NBDC NBDC NNP 10_1101-2021_01_07_425723 144 26 226 226 CD 10_1101-2021_01_07_425723 144 27 ( ( -LRB- 10_1101-2021_01_07_425723 144 28 < < XX 10_1101-2021_01_07_425723 144 29 2-fold 2-fold CD 10_1101-2021_01_07_425723 144 30 , , , 10_1101-2021_01_07_425723 144 31 Fig Fig NNP 10_1101-2021_01_07_425723 144 32 . . . 10_1101-2021_01_07_425723 145 1 4bc 4bc JJ 10_1101-2021_01_07_425723 145 2 , , , 10_1101-2021_01_07_425723 145 3 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 145 4 Fig Fig NNP 10_1101-2021_01_07_425723 145 5 . . . 10_1101-2021_01_07_425723 146 1 7 7 LS 10_1101-2021_01_07_425723 146 2 ) ) -RRB- 10_1101-2021_01_07_425723 146 3 . . . 10_1101-2021_01_07_425723 147 1 227 227 CD 10_1101-2021_01_07_425723 147 2 Fig fig NN 10_1101-2021_01_07_425723 147 3 . . . 10_1101-2021_01_07_425723 148 1 4 4 CD 10_1101-2021_01_07_425723 148 2 | | CD 10_1101-2021_01_07_425723 148 3 Uptake Uptake NNP 10_1101-2021_01_07_425723 148 4 of of IN 10_1101-2021_01_07_425723 148 5 the the DT 10_1101-2021_01_07_425723 148 6 fluorescent fluorescent NN 10_1101-2021_01_07_425723 148 7 sterol sterol NN 10_1101-2021_01_07_425723 148 8 derivative derivative JJ 10_1101-2021_01_07_425723 148 9 NBDC NBDC NNP 10_1101-2021_01_07_425723 148 10 by by IN 10_1101-2021_01_07_425723 148 11 S. S. NNP 10_1101-2021_01_07_425723 148 12 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 148 13 and and CC 10_1101-2021_01_07_425723 148 14 K. K. NNP 10_1101-2021_01_07_425723 148 15 marxianus marxianus NN 10_1101-2021_01_07_425723 148 16 strains strain VBZ 10_1101-2021_01_07_425723 148 17 . . . 10_1101-2021_01_07_425723 149 1 a a DT 10_1101-2021_01_07_425723 149 2 , , , 10_1101-2021_01_07_425723 149 3 228 228 CD 10_1101-2021_01_07_425723 149 4 Experimental experimental JJ 10_1101-2021_01_07_425723 149 5 approach approach NN 10_1101-2021_01_07_425723 149 6 . . . 10_1101-2021_01_07_425723 150 1 S. S. NNP 10_1101-2021_01_07_425723 150 2 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 150 3 strains strain VBZ 10_1101-2021_01_07_425723 150 4 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 150 5 ( ( -LRB- 10_1101-2021_01_07_425723 150 6 reference reference NN 10_1101-2021_01_07_425723 150 7 ) ) -RRB- 10_1101-2021_01_07_425723 150 8 and and CC 10_1101-2021_01_07_425723 150 9 IMK809 IMK809 NNP 10_1101-2021_01_07_425723 150 10 ( ( -LRB- 10_1101-2021_01_07_425723 150 11 aus1Δ aus1Δ NNP 10_1101-2021_01_07_425723 150 12 pdr11Δ pdr11Δ NNP 10_1101-2021_01_07_425723 150 13 ) ) -RRB- 10_1101-2021_01_07_425723 150 14 , , , 10_1101-2021_01_07_425723 150 15 and and CC 10_1101-2021_01_07_425723 150 16 K. K. NNP 10_1101-2021_01_07_425723 150 17 229 229 CD 10_1101-2021_01_07_425723 150 18 marxianus marxianus NN 10_1101-2021_01_07_425723 150 19 strains strain VBZ 10_1101-2021_01_07_425723 150 20 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 150 21 and and CC 10_1101-2021_01_07_425723 150 22 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 150 23 were be VBD 10_1101-2021_01_07_425723 150 24 each each DT 10_1101-2021_01_07_425723 150 25 anaerobically anaerobically RB 10_1101-2021_01_07_425723 150 26 incubated incubate VBN 10_1101-2021_01_07_425723 150 27 in in IN 10_1101-2021_01_07_425723 150 28 four four CD 10_1101-2021_01_07_425723 150 29 replicate replicate NN 10_1101-2021_01_07_425723 150 30 shake-230 shake-230 JJ 10_1101-2021_01_07_425723 150 31 .CC .CC NFP 10_1101-2021_01_07_425723 150 32 - - HYPH 10_1101-2021_01_07_425723 150 33 BY by IN 10_1101-2021_01_07_425723 150 34 - - HYPH 10_1101-2021_01_07_425723 150 35 NC NC NNP 10_1101-2021_01_07_425723 150 36 - - HYPH 10_1101-2021_01_07_425723 150 37 ND ND NNP 10_1101-2021_01_07_425723 150 38 4.0 4.0 CD 10_1101-2021_01_07_425723 150 39 International International NNP 10_1101-2021_01_07_425723 150 40 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 150 41 under under IN 10_1101-2021_01_07_425723 150 42 a a DT 10_1101-2021_01_07_425723 150 43 ( ( -LRB- 10_1101-2021_01_07_425723 150 44 which which WDT 10_1101-2021_01_07_425723 150 45 was be VBD 10_1101-2021_01_07_425723 150 46 not not RB 10_1101-2021_01_07_425723 150 47 certified certify VBN 10_1101-2021_01_07_425723 150 48 by by IN 10_1101-2021_01_07_425723 150 49 peer peer NN 10_1101-2021_01_07_425723 150 50 review review NN 10_1101-2021_01_07_425723 150 51 ) ) -RRB- 10_1101-2021_01_07_425723 150 52 is be VBZ 10_1101-2021_01_07_425723 150 53 the the DT 10_1101-2021_01_07_425723 150 54 author author NN 10_1101-2021_01_07_425723 150 55 / / SYM 10_1101-2021_01_07_425723 150 56 funder funder NN 10_1101-2021_01_07_425723 150 57 , , , 10_1101-2021_01_07_425723 150 58 who who WP 10_1101-2021_01_07_425723 150 59 has have VBZ 10_1101-2021_01_07_425723 150 60 granted grant VBN 10_1101-2021_01_07_425723 150 61 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 150 62 a a DT 10_1101-2021_01_07_425723 150 63 license license NN 10_1101-2021_01_07_425723 150 64 to to TO 10_1101-2021_01_07_425723 150 65 display display VB 10_1101-2021_01_07_425723 150 66 the the DT 10_1101-2021_01_07_425723 150 67 preprint preprint NN 10_1101-2021_01_07_425723 150 68 in in IN 10_1101-2021_01_07_425723 150 69 perpetuity perpetuity NN 10_1101-2021_01_07_425723 150 70 . . . 10_1101-2021_01_07_425723 151 1 It -PRON- PRP 10_1101-2021_01_07_425723 151 2 is be VBZ 10_1101-2021_01_07_425723 151 3 made make VBN 10_1101-2021_01_07_425723 151 4 The the DT 10_1101-2021_01_07_425723 151 5 copyright copyright NN 10_1101-2021_01_07_425723 151 6 holder holder NN 10_1101-2021_01_07_425723 151 7 for for IN 10_1101-2021_01_07_425723 151 8 this this DT 10_1101-2021_01_07_425723 151 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 151 10 version version NN 10_1101-2021_01_07_425723 151 11 posted post VBD 10_1101-2021_01_07_425723 151 12 January January NNP 10_1101-2021_01_07_425723 151 13 8 8 CD 10_1101-2021_01_07_425723 151 14 , , , 10_1101-2021_01_07_425723 151 15 2021 2021 CD 10_1101-2021_01_07_425723 151 16 . . . 10_1101-2021_01_07_425723 151 17 ; ; : 10_1101-2021_01_07_425723 151 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 151 19 : : : 10_1101-2021_01_07_425723 151 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 151 21 preprint preprint NN 10_1101-2021_01_07_425723 151 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 151 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425723 151 24 15 15 CD 10_1101-2021_01_07_425723 151 25 flask flask NN 10_1101-2021_01_07_425723 151 26 cultures culture NNS 10_1101-2021_01_07_425723 151 27 . . . 10_1101-2021_01_07_425723 152 1 NBDC NBDC NNP 10_1101-2021_01_07_425723 152 2 and and CC 10_1101-2021_01_07_425723 152 3 Tween tween NN 10_1101-2021_01_07_425723 152 4 80 80 CD 10_1101-2021_01_07_425723 152 5 ( ( -LRB- 10_1101-2021_01_07_425723 152 6 NBDC NBDC NNP 10_1101-2021_01_07_425723 152 7 T T NNP 10_1101-2021_01_07_425723 152 8 ) ) -RRB- 10_1101-2021_01_07_425723 152 9 were be VBD 10_1101-2021_01_07_425723 152 10 added add VBN 10_1101-2021_01_07_425723 152 11 to to IN 10_1101-2021_01_07_425723 152 12 two two CD 10_1101-2021_01_07_425723 152 13 cultures culture NNS 10_1101-2021_01_07_425723 152 14 , , , 10_1101-2021_01_07_425723 152 15 while while IN 10_1101-2021_01_07_425723 152 16 only only RB 10_1101-2021_01_07_425723 152 17 Tween Tween NNP 10_1101-2021_01_07_425723 152 18 80 80 CD 10_1101-2021_01_07_425723 152 19 ( ( -LRB- 10_1101-2021_01_07_425723 152 20 T T NNP 10_1101-2021_01_07_425723 152 21 ) ) -RRB- 10_1101-2021_01_07_425723 152 22 was be VBD 10_1101-2021_01_07_425723 152 23 231 231 CD 10_1101-2021_01_07_425723 152 24 added add VBN 10_1101-2021_01_07_425723 152 25 to to IN 10_1101-2021_01_07_425723 152 26 the the DT 10_1101-2021_01_07_425723 152 27 other other JJ 10_1101-2021_01_07_425723 152 28 two two CD 10_1101-2021_01_07_425723 152 29 . . . 10_1101-2021_01_07_425723 153 1 After after IN 10_1101-2021_01_07_425723 153 2 4 4 CD 10_1101-2021_01_07_425723 153 3 h h NN 10_1101-2021_01_07_425723 153 4 incubation incubation NN 10_1101-2021_01_07_425723 153 5 , , , 10_1101-2021_01_07_425723 153 6 cells cell NNS 10_1101-2021_01_07_425723 153 7 were be VBD 10_1101-2021_01_07_425723 153 8 stained stain VBN 10_1101-2021_01_07_425723 153 9 with with IN 10_1101-2021_01_07_425723 153 10 propidium propidium NN 10_1101-2021_01_07_425723 153 11 iodide iodide NN 10_1101-2021_01_07_425723 153 12 ( ( -LRB- 10_1101-2021_01_07_425723 153 13 PI PI NNP 10_1101-2021_01_07_425723 153 14 ) ) -RRB- 10_1101-2021_01_07_425723 153 15 and and CC 10_1101-2021_01_07_425723 153 16 analysed analyse VBD 10_1101-2021_01_07_425723 153 17 232 232 CD 10_1101-2021_01_07_425723 153 18 by by IN 10_1101-2021_01_07_425723 153 19 flow flow NN 10_1101-2021_01_07_425723 153 20 cytometry cytometry NN 10_1101-2021_01_07_425723 153 21 . . . 10_1101-2021_01_07_425723 154 1 PI PI NNP 10_1101-2021_01_07_425723 154 2 staining staining NN 10_1101-2021_01_07_425723 154 3 was be VBD 10_1101-2021_01_07_425723 154 4 used use VBN 10_1101-2021_01_07_425723 154 5 to to TO 10_1101-2021_01_07_425723 154 6 eliminate eliminate VB 10_1101-2021_01_07_425723 154 7 cells cell NNS 10_1101-2021_01_07_425723 154 8 with with IN 10_1101-2021_01_07_425723 154 9 compromised compromised JJ 10_1101-2021_01_07_425723 154 10 membrane membrane NN 10_1101-2021_01_07_425723 154 11 integrity integrity NN 10_1101-2021_01_07_425723 154 12 from from IN 10_1101-2021_01_07_425723 154 13 233 233 CD 10_1101-2021_01_07_425723 154 14 analysis analysis NN 10_1101-2021_01_07_425723 154 15 of of IN 10_1101-2021_01_07_425723 154 16 NBDC NBDC NNP 10_1101-2021_01_07_425723 154 17 fluorescence fluorescence NN 10_1101-2021_01_07_425723 154 18 . . . 10_1101-2021_01_07_425723 155 1 Cultivation cultivation NN 10_1101-2021_01_07_425723 155 2 conditions condition NNS 10_1101-2021_01_07_425723 155 3 and and CC 10_1101-2021_01_07_425723 155 4 flow flow NN 10_1101-2021_01_07_425723 155 5 cytometry cytometry NN 10_1101-2021_01_07_425723 155 6 gating gating NN 10_1101-2021_01_07_425723 155 7 are be VBP 10_1101-2021_01_07_425723 155 8 described describe VBN 10_1101-2021_01_07_425723 155 9 in in IN 10_1101-2021_01_07_425723 155 10 234 234 CD 10_1101-2021_01_07_425723 155 11 Methods Methods NNPS 10_1101-2021_01_07_425723 155 12 and and CC 10_1101-2021_01_07_425723 155 13 in in IN 10_1101-2021_01_07_425723 155 14 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 155 15 Fig Fig NNP 10_1101-2021_01_07_425723 155 16 . . . 10_1101-2021_01_07_425723 156 1 8 8 LS 10_1101-2021_01_07_425723 156 2 , , , 10_1101-2021_01_07_425723 156 3 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 156 4 Data Data NNP 10_1101-2021_01_07_425723 156 5 set set VBD 10_1101-2021_01_07_425723 156 6 1 1 CD 10_1101-2021_01_07_425723 156 7 and and CC 10_1101-2021_01_07_425723 156 8 2 2 CD 10_1101-2021_01_07_425723 156 9 . . . 10_1101-2021_01_07_425723 156 10 b b LS 10_1101-2021_01_07_425723 156 11 , , , 10_1101-2021_01_07_425723 156 12 Median Median NNP 10_1101-2021_01_07_425723 156 13 and and CC 10_1101-2021_01_07_425723 156 14 pooled pool VBD 10_1101-2021_01_07_425723 156 15 standard standard JJ 10_1101-2021_01_07_425723 156 16 235 235 CD 10_1101-2021_01_07_425723 156 17 deviation deviation NN 10_1101-2021_01_07_425723 156 18 of of IN 10_1101-2021_01_07_425723 156 19 fluorescence fluorescence NN 10_1101-2021_01_07_425723 156 20 intensity intensity NN 10_1101-2021_01_07_425723 156 21 ( ( -LRB- 10_1101-2021_01_07_425723 156 22 λex λex NNP 10_1101-2021_01_07_425723 156 23 488 488 CD 10_1101-2021_01_07_425723 156 24 nm nm NNP 10_1101-2021_01_07_425723 156 25 | | NNP 10_1101-2021_01_07_425723 156 26 λem λem NN 10_1101-2021_01_07_425723 156 27 533/30 533/30 CD 10_1101-2021_01_07_425723 156 28 nm nm RB 10_1101-2021_01_07_425723 156 29 , , , 10_1101-2021_01_07_425723 156 30 FL1-A FL1-A NNP 10_1101-2021_01_07_425723 156 31 ) ) -RRB- 10_1101-2021_01_07_425723 156 32 of of IN 10_1101-2021_01_07_425723 156 33 PI pi NN 10_1101-2021_01_07_425723 156 34 - - HYPH 10_1101-2021_01_07_425723 156 35 negative negative JJ 10_1101-2021_01_07_425723 156 36 cells cell NNS 10_1101-2021_01_07_425723 156 37 with with IN 10_1101-2021_01_07_425723 156 38 variance variance NN 10_1101-2021_01_07_425723 156 39 236 236 CD 10_1101-2021_01_07_425723 156 40 of of IN 10_1101-2021_01_07_425723 156 41 biological biological JJ 10_1101-2021_01_07_425723 156 42 replicates replicate NNS 10_1101-2021_01_07_425723 156 43 after after IN 10_1101-2021_01_07_425723 156 44 4 4 CD 10_1101-2021_01_07_425723 156 45 h h NN 10_1101-2021_01_07_425723 156 46 exposure exposure NN 10_1101-2021_01_07_425723 156 47 to to IN 10_1101-2021_01_07_425723 156 48 Tween Tween NNP 10_1101-2021_01_07_425723 156 49 80 80 CD 10_1101-2021_01_07_425723 156 50 ( ( -LRB- 10_1101-2021_01_07_425723 156 51 white white JJ 10_1101-2021_01_07_425723 156 52 bars bar NNS 10_1101-2021_01_07_425723 156 53 ) ) -RRB- 10_1101-2021_01_07_425723 156 54 or or CC 10_1101-2021_01_07_425723 156 55 Tween Tween NNP 10_1101-2021_01_07_425723 156 56 80 80 CD 10_1101-2021_01_07_425723 156 57 and and CC 10_1101-2021_01_07_425723 156 58 NBDC NBDC NNP 10_1101-2021_01_07_425723 156 59 ( ( -LRB- 10_1101-2021_01_07_425723 156 60 blue blue JJ 10_1101-2021_01_07_425723 156 61 bars bar NNS 10_1101-2021_01_07_425723 156 62 ) ) -RRB- 10_1101-2021_01_07_425723 156 63 . . . 10_1101-2021_01_07_425723 157 1 237 237 CD 10_1101-2021_01_07_425723 157 2 Variance variance NN 10_1101-2021_01_07_425723 157 3 was be VBD 10_1101-2021_01_07_425723 157 4 pooled pool VBN 10_1101-2021_01_07_425723 157 5 for for IN 10_1101-2021_01_07_425723 157 6 the the DT 10_1101-2021_01_07_425723 157 7 strains strain NNS 10_1101-2021_01_07_425723 157 8 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 157 9 , , , 10_1101-2021_01_07_425723 157 10 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 157 11 and and CC 10_1101-2021_01_07_425723 157 12 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 157 13 by by IN 10_1101-2021_01_07_425723 157 14 repeating repeat VBG 10_1101-2021_01_07_425723 157 15 the the DT 10_1101-2021_01_07_425723 157 16 experiment experiment NN 10_1101-2021_01_07_425723 157 17 . . . 10_1101-2021_01_07_425723 158 1 c c LS 10_1101-2021_01_07_425723 158 2 , , , 10_1101-2021_01_07_425723 158 3 238 238 CD 10_1101-2021_01_07_425723 158 4 NBDC NBDC NNP 10_1101-2021_01_07_425723 158 5 fluorescence fluorescence NN 10_1101-2021_01_07_425723 158 6 - - HYPH 10_1101-2021_01_07_425723 158 7 intensity intensity NN 10_1101-2021_01_07_425723 158 8 distribution distribution NN 10_1101-2021_01_07_425723 158 9 of of IN 10_1101-2021_01_07_425723 158 10 cells cell NNS 10_1101-2021_01_07_425723 158 11 in in IN 10_1101-2021_01_07_425723 158 12 a a DT 10_1101-2021_01_07_425723 158 13 sample sample NN 10_1101-2021_01_07_425723 158 14 from from IN 10_1101-2021_01_07_425723 158 15 a a DT 10_1101-2021_01_07_425723 158 16 single single JJ 10_1101-2021_01_07_425723 158 17 culture culture NN 10_1101-2021_01_07_425723 158 18 for for IN 10_1101-2021_01_07_425723 158 19 each each DT 10_1101-2021_01_07_425723 158 20 strain strain NN 10_1101-2021_01_07_425723 158 21 , , , 10_1101-2021_01_07_425723 158 22 shown show VBD 10_1101-2021_01_07_425723 158 23 239 239 CD 10_1101-2021_01_07_425723 158 24 as as IN 10_1101-2021_01_07_425723 158 25 modal modal NN 10_1101-2021_01_07_425723 158 26 - - HYPH 10_1101-2021_01_07_425723 158 27 scaled scale VBN 10_1101-2021_01_07_425723 158 28 density density NN 10_1101-2021_01_07_425723 158 29 function function NN 10_1101-2021_01_07_425723 158 30 . . . 10_1101-2021_01_07_425723 159 1 Dashed dash VBN 10_1101-2021_01_07_425723 159 2 lines line NNS 10_1101-2021_01_07_425723 159 3 represent represent VBP 10_1101-2021_01_07_425723 159 4 background background NN 10_1101-2021_01_07_425723 159 5 fluorescence fluorescence NN 10_1101-2021_01_07_425723 159 6 of of IN 10_1101-2021_01_07_425723 159 7 unstained unstained JJ 10_1101-2021_01_07_425723 159 8 cells cell NNS 10_1101-2021_01_07_425723 159 9 of of IN 10_1101-2021_01_07_425723 159 10 240 240 CD 10_1101-2021_01_07_425723 159 11 S. S. NNP 10_1101-2021_01_07_425723 159 12 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 159 13 and and CC 10_1101-2021_01_07_425723 159 14 K. K. NNP 10_1101-2021_01_07_425723 159 15 marxianus marxianus NN 10_1101-2021_01_07_425723 159 16 . . . 10_1101-2021_01_07_425723 160 1 Fluorescence fluorescence NN 10_1101-2021_01_07_425723 160 2 data datum NNS 10_1101-2021_01_07_425723 160 3 for for IN 10_1101-2021_01_07_425723 160 4 23-h 23-h CD 10_1101-2021_01_07_425723 160 5 incubations incubation NNS 10_1101-2021_01_07_425723 160 6 with with IN 10_1101-2021_01_07_425723 160 7 NBDC NBDC NNP 10_1101-2021_01_07_425723 160 8 are be VBP 10_1101-2021_01_07_425723 160 9 shown show VBN 10_1101-2021_01_07_425723 160 10 in in IN 10_1101-2021_01_07_425723 160 11 241 241 CD 10_1101-2021_01_07_425723 160 12 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 160 13 Fig Fig NNP 10_1101-2021_01_07_425723 160 14 . . . 10_1101-2021_01_07_425723 161 1 7 7 LS 10_1101-2021_01_07_425723 161 2 . . . 10_1101-2021_01_07_425723 162 1 242 242 CD 10_1101-2021_01_07_425723 162 2 Engineering Engineering NNP 10_1101-2021_01_07_425723 162 3 K. K. NNP 10_1101-2021_01_07_425723 162 4 marxianus marxianus NN 10_1101-2021_01_07_425723 162 5 for for IN 10_1101-2021_01_07_425723 162 6 oxygen oxygen NN 10_1101-2021_01_07_425723 162 7 - - HYPH 10_1101-2021_01_07_425723 162 8 independent independent JJ 10_1101-2021_01_07_425723 162 9 growth growth NN 10_1101-2021_01_07_425723 162 10 243 243 CD 10_1101-2021_01_07_425723 162 11 Sterol Sterol NNP 10_1101-2021_01_07_425723 162 12 uptake uptake NN 10_1101-2021_01_07_425723 162 13 by by IN 10_1101-2021_01_07_425723 162 14 S. S. NNP 10_1101-2021_01_07_425723 162 15 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 162 16 , , , 10_1101-2021_01_07_425723 162 17 which which WDT 10_1101-2021_01_07_425723 162 18 requires require VBZ 10_1101-2021_01_07_425723 162 19 cell cell NN 10_1101-2021_01_07_425723 162 20 wall wall NN 10_1101-2021_01_07_425723 162 21 proteins protein NNS 10_1101-2021_01_07_425723 162 22 as as RB 10_1101-2021_01_07_425723 162 23 well well RB 10_1101-2021_01_07_425723 162 24 as as IN 10_1101-2021_01_07_425723 162 25 a a DT 10_1101-2021_01_07_425723 162 26 membrane membrane NN 10_1101-2021_01_07_425723 162 27 transporter transporter NN 10_1101-2021_01_07_425723 162 28 , , , 10_1101-2021_01_07_425723 162 29 has have VBZ 10_1101-2021_01_07_425723 162 30 244 244 CD 10_1101-2021_01_07_425723 162 31 not not RB 10_1101-2021_01_07_425723 162 32 yet yet RB 10_1101-2021_01_07_425723 162 33 been be VBN 10_1101-2021_01_07_425723 162 34 fully fully RB 10_1101-2021_01_07_425723 162 35 resolved42,43 resolved42,43 JJ 10_1101-2021_01_07_425723 162 36 . . . 10_1101-2021_01_07_425723 163 1 Instead instead RB 10_1101-2021_01_07_425723 163 2 of of IN 10_1101-2021_01_07_425723 163 3 expressing express VBG 10_1101-2021_01_07_425723 163 4 a a DT 10_1101-2021_01_07_425723 163 5 heterologous heterologous JJ 10_1101-2021_01_07_425723 163 6 sterol sterol NN 10_1101-2021_01_07_425723 163 7 - - HYPH 10_1101-2021_01_07_425723 163 8 import import NN 10_1101-2021_01_07_425723 163 9 system system NN 10_1101-2021_01_07_425723 163 10 in in IN 10_1101-2021_01_07_425723 163 11 K. K. NNP 10_1101-2021_01_07_425723 163 12 245 245 CD 10_1101-2021_01_07_425723 163 13 marxianus marxianus NN 10_1101-2021_01_07_425723 163 14 , , , 10_1101-2021_01_07_425723 163 15 we -PRON- PRP 10_1101-2021_01_07_425723 163 16 therefore therefore RB 10_1101-2021_01_07_425723 163 17 explored explore VBD 10_1101-2021_01_07_425723 163 18 production production NN 10_1101-2021_01_07_425723 163 19 of of IN 10_1101-2021_01_07_425723 163 20 tetrahymanol tetrahymanol NNS 10_1101-2021_01_07_425723 163 21 , , , 10_1101-2021_01_07_425723 163 22 which which WDT 10_1101-2021_01_07_425723 163 23 acts act VBZ 10_1101-2021_01_07_425723 163 24 as as IN 10_1101-2021_01_07_425723 163 25 a a DT 10_1101-2021_01_07_425723 163 26 sterol sterol JJ 10_1101-2021_01_07_425723 163 27 surrogate surrogate NN 10_1101-2021_01_07_425723 163 28 in in IN 10_1101-2021_01_07_425723 163 29 246 246 CD 10_1101-2021_01_07_425723 163 30 strictly strictly RB 10_1101-2021_01_07_425723 163 31 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 163 32 fungi fungi NNP 10_1101-2021_01_07_425723 163 33 45 45 CD 10_1101-2021_01_07_425723 163 34 . . . 10_1101-2021_01_07_425723 164 1 Expression expression NN 10_1101-2021_01_07_425723 164 2 of of IN 10_1101-2021_01_07_425723 164 3 a a DT 10_1101-2021_01_07_425723 164 4 squalene squalene NN 10_1101-2021_01_07_425723 164 5 - - HYPH 10_1101-2021_01_07_425723 164 6 tetrahymanol tetrahymanol NN 10_1101-2021_01_07_425723 164 7 cyclase cyclase NN 10_1101-2021_01_07_425723 164 8 from from IN 10_1101-2021_01_07_425723 164 9 Tetrahymena Tetrahymena NNP 10_1101-2021_01_07_425723 164 10 247 247 CD 10_1101-2021_01_07_425723 164 11 thermophila thermophila NN 10_1101-2021_01_07_425723 164 12 ( ( -LRB- 10_1101-2021_01_07_425723 164 13 TtSTC1 ttstc1 NN 10_1101-2021_01_07_425723 164 14 ) ) -RRB- 10_1101-2021_01_07_425723 164 15 , , , 10_1101-2021_01_07_425723 164 16 which which WDT 10_1101-2021_01_07_425723 164 17 catalyzes catalyze VBZ 10_1101-2021_01_07_425723 164 18 the the DT 10_1101-2021_01_07_425723 164 19 single single JJ 10_1101-2021_01_07_425723 164 20 - - HYPH 10_1101-2021_01_07_425723 164 21 step step NN 10_1101-2021_01_07_425723 164 22 oxygen oxygen NN 10_1101-2021_01_07_425723 164 23 - - HYPH 10_1101-2021_01_07_425723 164 24 independent independent JJ 10_1101-2021_01_07_425723 164 25 conversion conversion NN 10_1101-2021_01_07_425723 164 26 of of IN 10_1101-2021_01_07_425723 164 27 squalene squalene NN 10_1101-2021_01_07_425723 164 28 into into IN 10_1101-2021_01_07_425723 164 29 248 248 CD 10_1101-2021_01_07_425723 164 30 tetrahymanol tetrahymanol NNS 10_1101-2021_01_07_425723 164 31 ( ( -LRB- 10_1101-2021_01_07_425723 164 32 Fig fig NN 10_1101-2021_01_07_425723 164 33 . . . 10_1101-2021_01_07_425723 165 1 5a 5a LS 10_1101-2021_01_07_425723 165 2 ) ) -RRB- 10_1101-2021_01_07_425723 165 3 , , , 10_1101-2021_01_07_425723 165 4 was be VBD 10_1101-2021_01_07_425723 165 5 recently recently RB 10_1101-2021_01_07_425723 165 6 shown show VBN 10_1101-2021_01_07_425723 165 7 to to TO 10_1101-2021_01_07_425723 165 8 enable enable VB 10_1101-2021_01_07_425723 165 9 sterol sterol JJ 10_1101-2021_01_07_425723 165 10 - - HYPH 10_1101-2021_01_07_425723 165 11 independent independent JJ 10_1101-2021_01_07_425723 165 12 growth growth NN 10_1101-2021_01_07_425723 165 13 of of IN 10_1101-2021_01_07_425723 165 14 S. S. NNP 10_1101-2021_01_07_425723 165 15 cerevisiae46 cerevisiae46 NNP 10_1101-2021_01_07_425723 165 16 . . . 10_1101-2021_01_07_425723 166 1 249 249 CD 10_1101-2021_01_07_425723 166 2 TtSTC1 ttstc1 CD 10_1101-2021_01_07_425723 166 3 was be VBD 10_1101-2021_01_07_425723 166 4 expressed express VBN 10_1101-2021_01_07_425723 166 5 in in IN 10_1101-2021_01_07_425723 166 6 K. K. NNP 10_1101-2021_01_07_425723 166 7 marxianus marxianus JJ 10_1101-2021_01_07_425723 166 8 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 166 9 , , , 10_1101-2021_01_07_425723 166 10 which which WDT 10_1101-2021_01_07_425723 166 11 is be VBZ 10_1101-2021_01_07_425723 166 12 more more RBR 10_1101-2021_01_07_425723 166 13 genetically genetically RB 10_1101-2021_01_07_425723 166 14 amenable amenable JJ 10_1101-2021_01_07_425723 166 15 than than IN 10_1101-2021_01_07_425723 166 16 strain strain VB 10_1101-2021_01_07_425723 166 17 250 250 CD 10_1101-2021_01_07_425723 166 18 CBS655647 cbs655647 NN 10_1101-2021_01_07_425723 166 19 . . . 10_1101-2021_01_07_425723 167 1 After after IN 10_1101-2021_01_07_425723 167 2 40 40 CD 10_1101-2021_01_07_425723 167 3 h h NN 10_1101-2021_01_07_425723 167 4 of of IN 10_1101-2021_01_07_425723 167 5 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 167 6 incubation incubation NN 10_1101-2021_01_07_425723 167 7 , , , 10_1101-2021_01_07_425723 167 8 the the DT 10_1101-2021_01_07_425723 167 9 resulting result VBG 10_1101-2021_01_07_425723 167 10 strain strain NN 10_1101-2021_01_07_425723 167 11 contained contain VBD 10_1101-2021_01_07_425723 167 12 2.4 2.4 CD 10_1101-2021_01_07_425723 167 13 ± ± CD 10_1101-2021_01_07_425723 167 14 0.4 0.4 CD 10_1101-2021_01_07_425723 167 15 mg·(g mg·(g NNP 10_1101-2021_01_07_425723 167 16 biomass)-1 biomass)-1 CD 10_1101-2021_01_07_425723 167 17 251 251 CD 10_1101-2021_01_07_425723 167 18 tetrahymanol tetrahymanol NNS 10_1101-2021_01_07_425723 167 19 , , , 10_1101-2021_01_07_425723 167 20 0.4 0.4 CD 10_1101-2021_01_07_425723 167 21 ± ± CD 10_1101-2021_01_07_425723 167 22 0.1 0.1 CD 10_1101-2021_01_07_425723 167 23 mg·g-1 mg·g-1 NNP 10_1101-2021_01_07_425723 167 24 ergosterol ergosterol UH 10_1101-2021_01_07_425723 167 25 and and CC 10_1101-2021_01_07_425723 167 26 no no DT 10_1101-2021_01_07_425723 167 27 detectable detectable JJ 10_1101-2021_01_07_425723 167 28 squalene squalene NN 10_1101-2021_01_07_425723 167 29 , , , 10_1101-2021_01_07_425723 167 30 while while IN 10_1101-2021_01_07_425723 167 31 strain strain NN 10_1101-2021_01_07_425723 167 32 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 167 33 contained contain VBD 10_1101-2021_01_07_425723 167 34 252 252 CD 10_1101-2021_01_07_425723 167 35 3.5 3.5 CD 10_1101-2021_01_07_425723 167 36 ± ± CD 10_1101-2021_01_07_425723 167 37 0.1 0.1 CD 10_1101-2021_01_07_425723 167 38 mg·g-1 mg·g-1 NN 10_1101-2021_01_07_425723 167 39 squalene squalene NN 10_1101-2021_01_07_425723 167 40 and and CC 10_1101-2021_01_07_425723 167 41 3.4 3.4 CD 10_1101-2021_01_07_425723 167 42 ± ± CD 10_1101-2021_01_07_425723 167 43 0.2 0.2 CD 10_1101-2021_01_07_425723 167 44 mg·g-1 mg·g-1 NN 10_1101-2021_01_07_425723 167 45 ergosterol ergosterol NN 10_1101-2021_01_07_425723 167 46 ( ( -LRB- 10_1101-2021_01_07_425723 167 47 Fig fig NN 10_1101-2021_01_07_425723 167 48 . . . 10_1101-2021_01_07_425723 168 1 5b 5b NN 10_1101-2021_01_07_425723 168 2 ) ) -RRB- 10_1101-2021_01_07_425723 168 3 . . . 10_1101-2021_01_07_425723 169 1 In in IN 10_1101-2021_01_07_425723 169 2 strictly strictly RB 10_1101-2021_01_07_425723 169 3 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 169 4 cultures culture NNS 10_1101-2021_01_07_425723 169 5 on on IN 10_1101-2021_01_07_425723 169 6 sterol-253 sterol-253 NNP 10_1101-2021_01_07_425723 169 7 .CC .CC : 10_1101-2021_01_07_425723 169 8 - - HYPH 10_1101-2021_01_07_425723 169 9 BY by IN 10_1101-2021_01_07_425723 169 10 - - HYPH 10_1101-2021_01_07_425723 169 11 NC NC NNP 10_1101-2021_01_07_425723 169 12 - - HYPH 10_1101-2021_01_07_425723 169 13 ND ND NNP 10_1101-2021_01_07_425723 169 14 4.0 4.0 CD 10_1101-2021_01_07_425723 169 15 International International NNP 10_1101-2021_01_07_425723 169 16 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 169 17 under under IN 10_1101-2021_01_07_425723 169 18 a a DT 10_1101-2021_01_07_425723 169 19 ( ( -LRB- 10_1101-2021_01_07_425723 169 20 which which WDT 10_1101-2021_01_07_425723 169 21 was be VBD 10_1101-2021_01_07_425723 169 22 not not RB 10_1101-2021_01_07_425723 169 23 certified certify VBN 10_1101-2021_01_07_425723 169 24 by by IN 10_1101-2021_01_07_425723 169 25 peer peer NN 10_1101-2021_01_07_425723 169 26 review review NN 10_1101-2021_01_07_425723 169 27 ) ) -RRB- 10_1101-2021_01_07_425723 169 28 is be VBZ 10_1101-2021_01_07_425723 169 29 the the DT 10_1101-2021_01_07_425723 169 30 author author NN 10_1101-2021_01_07_425723 169 31 / / SYM 10_1101-2021_01_07_425723 169 32 funder funder NN 10_1101-2021_01_07_425723 169 33 , , , 10_1101-2021_01_07_425723 169 34 who who WP 10_1101-2021_01_07_425723 169 35 has have VBZ 10_1101-2021_01_07_425723 169 36 granted grant VBN 10_1101-2021_01_07_425723 169 37 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 169 38 a a DT 10_1101-2021_01_07_425723 169 39 license license NN 10_1101-2021_01_07_425723 169 40 to to TO 10_1101-2021_01_07_425723 169 41 display display VB 10_1101-2021_01_07_425723 169 42 the the DT 10_1101-2021_01_07_425723 169 43 preprint preprint NN 10_1101-2021_01_07_425723 169 44 in in IN 10_1101-2021_01_07_425723 169 45 perpetuity perpetuity NN 10_1101-2021_01_07_425723 169 46 . . . 10_1101-2021_01_07_425723 170 1 It -PRON- PRP 10_1101-2021_01_07_425723 170 2 is be VBZ 10_1101-2021_01_07_425723 170 3 made make VBN 10_1101-2021_01_07_425723 170 4 The the DT 10_1101-2021_01_07_425723 170 5 copyright copyright NN 10_1101-2021_01_07_425723 170 6 holder holder NN 10_1101-2021_01_07_425723 170 7 for for IN 10_1101-2021_01_07_425723 170 8 this this DT 10_1101-2021_01_07_425723 170 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 170 10 version version NN 10_1101-2021_01_07_425723 170 11 posted post VBD 10_1101-2021_01_07_425723 170 12 January January NNP 10_1101-2021_01_07_425723 170 13 8 8 CD 10_1101-2021_01_07_425723 170 14 , , , 10_1101-2021_01_07_425723 170 15 2021 2021 CD 10_1101-2021_01_07_425723 170 16 . . . 10_1101-2021_01_07_425723 170 17 ; ; : 10_1101-2021_01_07_425723 170 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 170 19 : : : 10_1101-2021_01_07_425723 170 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 170 21 preprint preprint NN 10_1101-2021_01_07_425723 170 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 170 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425723 170 24 16 16 CD 10_1101-2021_01_07_425723 170 25 free free JJ 10_1101-2021_01_07_425723 170 26 medium medium NN 10_1101-2021_01_07_425723 170 27 , , , 10_1101-2021_01_07_425723 170 28 strain strain NN 10_1101-2021_01_07_425723 170 29 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 170 30 grew grow VBD 10_1101-2021_01_07_425723 170 31 immediately immediately RB 10_1101-2021_01_07_425723 170 32 after after IN 10_1101-2021_01_07_425723 170 33 inoculation inoculation NN 10_1101-2021_01_07_425723 170 34 but but CC 10_1101-2021_01_07_425723 170 35 not not RB 10_1101-2021_01_07_425723 170 36 after after IN 10_1101-2021_01_07_425723 170 37 transfer transfer NN 10_1101-2021_01_07_425723 170 38 to to IN 10_1101-2021_01_07_425723 170 39 a a DT 10_1101-2021_01_07_425723 170 40 second second JJ 10_1101-2021_01_07_425723 170 41 254 254 CD 10_1101-2021_01_07_425723 170 42 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 170 43 culture culture NN 10_1101-2021_01_07_425723 170 44 ( ( -LRB- 10_1101-2021_01_07_425723 170 45 Fig fig NN 10_1101-2021_01_07_425723 170 46 . . . 10_1101-2021_01_07_425723 171 1 5c 5c LS 10_1101-2021_01_07_425723 171 2 ) ) -RRB- 10_1101-2021_01_07_425723 171 3 , , , 10_1101-2021_01_07_425723 171 4 consistent consistent JJ 10_1101-2021_01_07_425723 171 5 with with IN 10_1101-2021_01_07_425723 171 6 ‘ ' `` 10_1101-2021_01_07_425723 171 7 carry carry VB 10_1101-2021_01_07_425723 171 8 - - HYPH 10_1101-2021_01_07_425723 171 9 over over RP 10_1101-2021_01_07_425723 171 10 ’ ' '' 10_1101-2021_01_07_425723 171 11 of of IN 10_1101-2021_01_07_425723 171 12 ergosterol ergosterol NN 10_1101-2021_01_07_425723 171 13 from from IN 10_1101-2021_01_07_425723 171 14 the the DT 10_1101-2021_01_07_425723 171 15 aerobic aerobic JJ 10_1101-2021_01_07_425723 171 16 preculture19 preculture19 NN 10_1101-2021_01_07_425723 171 17 . . . 10_1101-2021_01_07_425723 172 1 The the DT 10_1101-2021_01_07_425723 172 2 255 255 CD 10_1101-2021_01_07_425723 172 3 tetrahymanol tetrahymanol NN 10_1101-2021_01_07_425723 172 4 - - HYPH 10_1101-2021_01_07_425723 172 5 producing produce VBG 10_1101-2021_01_07_425723 172 6 strain strain NN 10_1101-2021_01_07_425723 172 7 did do VBD 10_1101-2021_01_07_425723 172 8 not not RB 10_1101-2021_01_07_425723 172 9 grow grow VB 10_1101-2021_01_07_425723 172 10 under under IN 10_1101-2021_01_07_425723 172 11 these these DT 10_1101-2021_01_07_425723 172 12 conditions condition NNS 10_1101-2021_01_07_425723 172 13 ( ( -LRB- 10_1101-2021_01_07_425723 172 14 Fig fig NN 10_1101-2021_01_07_425723 172 15 . . . 10_1101-2021_01_07_425723 173 1 5c 5c LS 10_1101-2021_01_07_425723 173 2 ) ) -RRB- 10_1101-2021_01_07_425723 173 3 but but CC 10_1101-2021_01_07_425723 173 4 showed show VBD 10_1101-2021_01_07_425723 173 5 sustained sustained JJ 10_1101-2021_01_07_425723 173 6 256 256 CD 10_1101-2021_01_07_425723 173 7 growth growth NN 10_1101-2021_01_07_425723 173 8 under under IN 10_1101-2021_01_07_425723 173 9 severely severely RB 10_1101-2021_01_07_425723 173 10 oxygen oxygen NN 10_1101-2021_01_07_425723 173 11 - - HYPH 10_1101-2021_01_07_425723 173 12 limited limit VBN 10_1101-2021_01_07_425723 173 13 conditions condition NNS 10_1101-2021_01_07_425723 173 14 that that WDT 10_1101-2021_01_07_425723 173 15 did do VBD 10_1101-2021_01_07_425723 173 16 not not RB 10_1101-2021_01_07_425723 173 17 support support VB 10_1101-2021_01_07_425723 173 18 growth growth NN 10_1101-2021_01_07_425723 173 19 of of IN 10_1101-2021_01_07_425723 173 20 strain strain NN 10_1101-2021_01_07_425723 173 21 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 173 22 ( ( -LRB- 10_1101-2021_01_07_425723 173 23 Fig fig NN 10_1101-2021_01_07_425723 173 24 . . . 10_1101-2021_01_07_425723 174 1 257 257 CD 10_1101-2021_01_07_425723 174 2 5de 5de NNP 10_1101-2021_01_07_425723 174 3 ) ) -RRB- 10_1101-2021_01_07_425723 174 4 . . . 10_1101-2021_01_07_425723 175 1 Single single JJ 10_1101-2021_01_07_425723 175 2 - - HYPH 10_1101-2021_01_07_425723 175 3 cell cell NN 10_1101-2021_01_07_425723 175 4 isolates isolate NNS 10_1101-2021_01_07_425723 175 5 derived derive VBN 10_1101-2021_01_07_425723 175 6 from from IN 10_1101-2021_01_07_425723 175 7 these these DT 10_1101-2021_01_07_425723 175 8 oxygen oxygen NN 10_1101-2021_01_07_425723 175 9 - - HYPH 10_1101-2021_01_07_425723 175 10 limited limit VBN 10_1101-2021_01_07_425723 175 11 cultures culture NNS 10_1101-2021_01_07_425723 175 12 ( ( -LRB- 10_1101-2021_01_07_425723 175 13 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 175 14 , , , 10_1101-2021_01_07_425723 175 15 IMS1131 IMS1131 NNP 10_1101-2021_01_07_425723 175 16 , , , 10_1101-2021_01_07_425723 175 17 IMS1132 IMS1132 NNP 10_1101-2021_01_07_425723 175 18 , , , 10_1101-2021_01_07_425723 175 19 258 258 CD 10_1101-2021_01_07_425723 175 20 IMS1133 IMS1133 NNP 10_1101-2021_01_07_425723 175 21 ) ) -RRB- 10_1101-2021_01_07_425723 175 22 showed show VBD 10_1101-2021_01_07_425723 175 23 instantaneous instantaneous JJ 10_1101-2021_01_07_425723 175 24 as as RB 10_1101-2021_01_07_425723 175 25 well well RB 10_1101-2021_01_07_425723 175 26 as as IN 10_1101-2021_01_07_425723 175 27 sustained sustain VBN 10_1101-2021_01_07_425723 175 28 growth growth NN 10_1101-2021_01_07_425723 175 29 under under IN 10_1101-2021_01_07_425723 175 30 strictly strictly RB 10_1101-2021_01_07_425723 175 31 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 175 32 conditions condition NNS 10_1101-2021_01_07_425723 175 33 ( ( -LRB- 10_1101-2021_01_07_425723 175 34 Figure Figure NNP 10_1101-2021_01_07_425723 175 35 259 259 CD 10_1101-2021_01_07_425723 175 36 5f 5f NNS 10_1101-2021_01_07_425723 175 37 and and CC 10_1101-2021_01_07_425723 175 38 5 5 CD 10_1101-2021_01_07_425723 175 39 g g NN 10_1101-2021_01_07_425723 175 40 ) ) -RRB- 10_1101-2021_01_07_425723 175 41 . . . 10_1101-2021_01_07_425723 176 1 Tetrahymanol tetrahymanol NN 10_1101-2021_01_07_425723 176 2 contents content VBZ 10_1101-2021_01_07_425723 176 3 in in IN 10_1101-2021_01_07_425723 176 4 the the DT 10_1101-2021_01_07_425723 176 5 first first JJ 10_1101-2021_01_07_425723 176 6 , , , 10_1101-2021_01_07_425723 176 7 second second JJ 10_1101-2021_01_07_425723 176 8 and and CC 10_1101-2021_01_07_425723 176 9 third third JJ 10_1101-2021_01_07_425723 176 10 cycle cycle NN 10_1101-2021_01_07_425723 176 11 of of IN 10_1101-2021_01_07_425723 176 12 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 176 13 cultivation cultivation NN 10_1101-2021_01_07_425723 176 14 of of IN 10_1101-2021_01_07_425723 176 15 isolate isolate NN 10_1101-2021_01_07_425723 176 16 260 260 CD 10_1101-2021_01_07_425723 176 17 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 176 18 were be VBD 10_1101-2021_01_07_425723 176 19 7.6 7.6 CD 10_1101-2021_01_07_425723 176 20 ± ± CD 10_1101-2021_01_07_425723 176 21 0.0 0.0 CD 10_1101-2021_01_07_425723 176 22 mg·g-1 mg·g-1 CD 10_1101-2021_01_07_425723 176 23 , , , 10_1101-2021_01_07_425723 176 24 28.0 28.0 CD 10_1101-2021_01_07_425723 176 25 ± ± CD 10_1101-2021_01_07_425723 176 26 13.0 13.0 CD 10_1101-2021_01_07_425723 176 27 mg·g-1 mg·g-1 NNP 10_1101-2021_01_07_425723 176 28 and and CC 10_1101-2021_01_07_425723 176 29 11.5 11.5 CD 10_1101-2021_01_07_425723 176 30 ± ± CD 10_1101-2021_01_07_425723 176 31 0.1 0.1 CD 10_1101-2021_01_07_425723 176 32 mg·g-1 mg·g-1 NNP 10_1101-2021_01_07_425723 176 33 , , , 10_1101-2021_01_07_425723 176 34 respectively respectively RB 10_1101-2021_01_07_425723 176 35 ( ( -LRB- 10_1101-2021_01_07_425723 176 36 Fig fig NN 10_1101-2021_01_07_425723 176 37 . . . 10_1101-2021_01_07_425723 177 1 5b 5b NN 10_1101-2021_01_07_425723 177 2 ) ) -RRB- 10_1101-2021_01_07_425723 177 3 , , , 10_1101-2021_01_07_425723 177 4 while while IN 10_1101-2021_01_07_425723 177 5 no no DT 10_1101-2021_01_07_425723 177 6 261 261 CD 10_1101-2021_01_07_425723 177 7 ergosterol ergosterol NN 10_1101-2021_01_07_425723 177 8 was be VBD 10_1101-2021_01_07_425723 177 9 detected detect VBN 10_1101-2021_01_07_425723 177 10 . . . 10_1101-2021_01_07_425723 178 1 262 262 CD 10_1101-2021_01_07_425723 178 2 To to TO 10_1101-2021_01_07_425723 178 3 identify identify VB 10_1101-2021_01_07_425723 178 4 whether whether IN 10_1101-2021_01_07_425723 178 5 adaptation adaptation NN 10_1101-2021_01_07_425723 178 6 of of IN 10_1101-2021_01_07_425723 178 7 the the DT 10_1101-2021_01_07_425723 178 8 tetrahymanol tetrahymanol NN 10_1101-2021_01_07_425723 178 9 - - HYPH 10_1101-2021_01_07_425723 178 10 producing produce VBG 10_1101-2021_01_07_425723 178 11 strain strain NN 10_1101-2021_01_07_425723 178 12 IMX2323 IMX2323 NNP 10_1101-2021_01_07_425723 178 13 to to TO 10_1101-2021_01_07_425723 178 14 anaerobic anaerobic VB 10_1101-2021_01_07_425723 178 15 growth growth NN 10_1101-2021_01_07_425723 178 16 263 263 CD 10_1101-2021_01_07_425723 178 17 involved involve VBD 10_1101-2021_01_07_425723 178 18 genetic genetic JJ 10_1101-2021_01_07_425723 178 19 changes change NNS 10_1101-2021_01_07_425723 178 20 , , , 10_1101-2021_01_07_425723 178 21 its -PRON- PRP$ 10_1101-2021_01_07_425723 178 22 genome genome NN 10_1101-2021_01_07_425723 178 23 and and CC 10_1101-2021_01_07_425723 178 24 those those DT 10_1101-2021_01_07_425723 178 25 of of IN 10_1101-2021_01_07_425723 178 26 the the DT 10_1101-2021_01_07_425723 178 27 four four CD 10_1101-2021_01_07_425723 178 28 adapted adapt VBN 10_1101-2021_01_07_425723 178 29 isolates isolate NNS 10_1101-2021_01_07_425723 178 30 were be VBD 10_1101-2021_01_07_425723 178 31 sequenced sequence VBN 10_1101-2021_01_07_425723 178 32 264 264 CD 10_1101-2021_01_07_425723 178 33 ( ( -LRB- 10_1101-2021_01_07_425723 178 34 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 178 35 Table Table NNP 10_1101-2021_01_07_425723 178 36 1 1 CD 10_1101-2021_01_07_425723 178 37 ) ) -RRB- 10_1101-2021_01_07_425723 178 38 . . . 10_1101-2021_01_07_425723 179 1 No no DT 10_1101-2021_01_07_425723 179 2 copy copy NN 10_1101-2021_01_07_425723 179 3 number number NN 10_1101-2021_01_07_425723 179 4 variations variation NNS 10_1101-2021_01_07_425723 179 5 were be VBD 10_1101-2021_01_07_425723 179 6 detected detect VBN 10_1101-2021_01_07_425723 179 7 in in IN 10_1101-2021_01_07_425723 179 8 any any DT 10_1101-2021_01_07_425723 179 9 of of IN 10_1101-2021_01_07_425723 179 10 the the DT 10_1101-2021_01_07_425723 179 11 four four CD 10_1101-2021_01_07_425723 179 12 adapted adapt VBN 10_1101-2021_01_07_425723 179 13 isolates isolate NNS 10_1101-2021_01_07_425723 179 14 . . . 10_1101-2021_01_07_425723 180 1 265 265 CD 10_1101-2021_01_07_425723 180 2 Only only RB 10_1101-2021_01_07_425723 180 3 strain strain VB 10_1101-2021_01_07_425723 180 4 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 180 5 showed show VBD 10_1101-2021_01_07_425723 180 6 two two CD 10_1101-2021_01_07_425723 180 7 non non JJ 10_1101-2021_01_07_425723 180 8 - - JJ 10_1101-2021_01_07_425723 180 9 conservative conservative JJ 10_1101-2021_01_07_425723 180 10 mutations mutation NNS 10_1101-2021_01_07_425723 180 11 in in IN 10_1101-2021_01_07_425723 180 12 coding code VBG 10_1101-2021_01_07_425723 180 13 regions region NNS 10_1101-2021_01_07_425723 180 14 : : : 10_1101-2021_01_07_425723 180 15 a a DT 10_1101-2021_01_07_425723 180 16 single single JJ 10_1101-2021_01_07_425723 180 17 - - HYPH 10_1101-2021_01_07_425723 180 18 nucleotide nucleotide NN 10_1101-2021_01_07_425723 180 19 266 266 CD 10_1101-2021_01_07_425723 180 20 insertion insertion NN 10_1101-2021_01_07_425723 180 21 in in IN 10_1101-2021_01_07_425723 180 22 a a DT 10_1101-2021_01_07_425723 180 23 transposon transposon NNP 10_1101-2021_01_07_425723 180 24 - - HYPH 10_1101-2021_01_07_425723 180 25 borne bear VBN 10_1101-2021_01_07_425723 180 26 gene gene NN 10_1101-2021_01_07_425723 180 27 and and CC 10_1101-2021_01_07_425723 180 28 a a DT 10_1101-2021_01_07_425723 180 29 stop stop NN 10_1101-2021_01_07_425723 180 30 codon codon NN 10_1101-2021_01_07_425723 180 31 at at IN 10_1101-2021_01_07_425723 180 32 position position NN 10_1101-2021_01_07_425723 180 33 350 350 CD 10_1101-2021_01_07_425723 180 34 ( ( -LRB- 10_1101-2021_01_07_425723 180 35 of of IN 10_1101-2021_01_07_425723 180 36 496 496 CD 10_1101-2021_01_07_425723 180 37 bp bp NN 10_1101-2021_01_07_425723 180 38 ) ) -RRB- 10_1101-2021_01_07_425723 180 39 in in IN 10_1101-2021_01_07_425723 180 40 KmCLN3 KmCLN3 NNP 10_1101-2021_01_07_425723 180 41 , , , 10_1101-2021_01_07_425723 180 42 which which WDT 10_1101-2021_01_07_425723 180 43 267 267 CD 10_1101-2021_01_07_425723 180 44 encodes encode NNS 10_1101-2021_01_07_425723 180 45 for for IN 10_1101-2021_01_07_425723 180 46 a a DT 10_1101-2021_01_07_425723 180 47 G1 g1 NN 10_1101-2021_01_07_425723 180 48 cyclin48 cyclin48 NN 10_1101-2021_01_07_425723 180 49 . . . 10_1101-2021_01_07_425723 181 1 The the DT 10_1101-2021_01_07_425723 181 2 apparent apparent JJ 10_1101-2021_01_07_425723 181 3 absence absence NN 10_1101-2021_01_07_425723 181 4 of of IN 10_1101-2021_01_07_425723 181 5 mutations mutation NNS 10_1101-2021_01_07_425723 181 6 in in IN 10_1101-2021_01_07_425723 181 7 the the DT 10_1101-2021_01_07_425723 181 8 three three CD 10_1101-2021_01_07_425723 181 9 other other JJ 10_1101-2021_01_07_425723 181 10 , , , 10_1101-2021_01_07_425723 181 11 independently independently RB 10_1101-2021_01_07_425723 181 12 adapted adapt VBD 10_1101-2021_01_07_425723 181 13 268 268 CD 10_1101-2021_01_07_425723 181 14 strains strain NNS 10_1101-2021_01_07_425723 181 15 indicated indicate VBD 10_1101-2021_01_07_425723 181 16 that that IN 10_1101-2021_01_07_425723 181 17 their -PRON- PRP$ 10_1101-2021_01_07_425723 181 18 ability ability NN 10_1101-2021_01_07_425723 181 19 to to TO 10_1101-2021_01_07_425723 181 20 grow grow VB 10_1101-2021_01_07_425723 181 21 anaerobically anaerobically RB 10_1101-2021_01_07_425723 181 22 reflected reflect VBN 10_1101-2021_01_07_425723 181 23 a a DT 10_1101-2021_01_07_425723 181 24 non non JJ 10_1101-2021_01_07_425723 181 25 - - JJ 10_1101-2021_01_07_425723 181 26 genetic genetic JJ 10_1101-2021_01_07_425723 181 27 adaptation adaptation NN 10_1101-2021_01_07_425723 181 28 . . . 10_1101-2021_01_07_425723 182 1 269 269 CD 10_1101-2021_01_07_425723 182 2 .CC .CC : 10_1101-2021_01_07_425723 182 3 - - HYPH 10_1101-2021_01_07_425723 182 4 BY by IN 10_1101-2021_01_07_425723 182 5 - - HYPH 10_1101-2021_01_07_425723 182 6 NC NC NNP 10_1101-2021_01_07_425723 182 7 - - HYPH 10_1101-2021_01_07_425723 182 8 ND ND NNP 10_1101-2021_01_07_425723 182 9 4.0 4.0 CD 10_1101-2021_01_07_425723 182 10 International International NNP 10_1101-2021_01_07_425723 182 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 182 12 under under IN 10_1101-2021_01_07_425723 182 13 a a DT 10_1101-2021_01_07_425723 182 14 ( ( -LRB- 10_1101-2021_01_07_425723 182 15 which which WDT 10_1101-2021_01_07_425723 182 16 was be VBD 10_1101-2021_01_07_425723 182 17 not not RB 10_1101-2021_01_07_425723 182 18 certified certify VBN 10_1101-2021_01_07_425723 182 19 by by IN 10_1101-2021_01_07_425723 182 20 peer peer NN 10_1101-2021_01_07_425723 182 21 review review NN 10_1101-2021_01_07_425723 182 22 ) ) -RRB- 10_1101-2021_01_07_425723 182 23 is be VBZ 10_1101-2021_01_07_425723 182 24 the the DT 10_1101-2021_01_07_425723 182 25 author author NN 10_1101-2021_01_07_425723 182 26 / / SYM 10_1101-2021_01_07_425723 182 27 funder funder NN 10_1101-2021_01_07_425723 182 28 , , , 10_1101-2021_01_07_425723 182 29 who who WP 10_1101-2021_01_07_425723 182 30 has have VBZ 10_1101-2021_01_07_425723 182 31 granted grant VBN 10_1101-2021_01_07_425723 182 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 182 33 a a DT 10_1101-2021_01_07_425723 182 34 license license NN 10_1101-2021_01_07_425723 182 35 to to TO 10_1101-2021_01_07_425723 182 36 display display VB 10_1101-2021_01_07_425723 182 37 the the DT 10_1101-2021_01_07_425723 182 38 preprint preprint NN 10_1101-2021_01_07_425723 182 39 in in IN 10_1101-2021_01_07_425723 182 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 182 41 . . . 10_1101-2021_01_07_425723 183 1 It -PRON- PRP 10_1101-2021_01_07_425723 183 2 is be VBZ 10_1101-2021_01_07_425723 183 3 made make VBN 10_1101-2021_01_07_425723 183 4 The the DT 10_1101-2021_01_07_425723 183 5 copyright copyright NN 10_1101-2021_01_07_425723 183 6 holder holder NN 10_1101-2021_01_07_425723 183 7 for for IN 10_1101-2021_01_07_425723 183 8 this this DT 10_1101-2021_01_07_425723 183 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 183 10 version version NN 10_1101-2021_01_07_425723 183 11 posted post VBD 10_1101-2021_01_07_425723 183 12 January January NNP 10_1101-2021_01_07_425723 183 13 8 8 CD 10_1101-2021_01_07_425723 183 14 , , , 10_1101-2021_01_07_425723 183 15 2021 2021 CD 10_1101-2021_01_07_425723 183 16 . . . 10_1101-2021_01_07_425723 183 17 ; ; : 10_1101-2021_01_07_425723 183 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 183 19 : : : 10_1101-2021_01_07_425723 183 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 183 21 preprint preprint NN 10_1101-2021_01_07_425723 183 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 183 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 183 24 17 17 CD 10_1101-2021_01_07_425723 183 25 Fig Fig NNP 10_1101-2021_01_07_425723 183 26 . . . 10_1101-2021_01_07_425723 184 1 5 5 CD 10_1101-2021_01_07_425723 184 2 | | NNP 10_1101-2021_01_07_425723 184 3 Sterol Sterol NNP 10_1101-2021_01_07_425723 184 4 - - HYPH 10_1101-2021_01_07_425723 184 5 independent independent JJ 10_1101-2021_01_07_425723 184 6 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 184 7 growth growth NN 10_1101-2021_01_07_425723 184 8 of of IN 10_1101-2021_01_07_425723 184 9 K. K. NNP 10_1101-2021_01_07_425723 184 10 marxianus marxianus NN 10_1101-2021_01_07_425723 184 11 strains strain VBZ 10_1101-2021_01_07_425723 184 12 expressing express VBG 10_1101-2021_01_07_425723 184 13 TtSTC1 ttstc1 CD 10_1101-2021_01_07_425723 184 14 . . . 10_1101-2021_01_07_425723 185 1 a a LS 10_1101-2021_01_07_425723 185 2 , , , 10_1101-2021_01_07_425723 185 3 Oxygen-270 oxygen-270 ADD 10_1101-2021_01_07_425723 185 4 dependent dependent JJ 10_1101-2021_01_07_425723 185 5 sterol sterol JJ 10_1101-2021_01_07_425723 185 6 synthesis synthesis NN 10_1101-2021_01_07_425723 185 7 and and CC 10_1101-2021_01_07_425723 185 8 cyclisation cyclisation NN 10_1101-2021_01_07_425723 185 9 of of IN 10_1101-2021_01_07_425723 185 10 squalene squalene NN 10_1101-2021_01_07_425723 185 11 to to IN 10_1101-2021_01_07_425723 185 12 tetrahymanol tetrahymanol NNS 10_1101-2021_01_07_425723 185 13 by by IN 10_1101-2021_01_07_425723 185 14 TtStc1 ttstc1 NN 10_1101-2021_01_07_425723 185 15 . . . 10_1101-2021_01_07_425723 186 1 b b LS 10_1101-2021_01_07_425723 186 2 , , , 10_1101-2021_01_07_425723 186 3 Squalene Squalene NNP 10_1101-2021_01_07_425723 186 4 , , , 10_1101-2021_01_07_425723 186 5 271 271 CD 10_1101-2021_01_07_425723 186 6 ergosterol ergosterol NN 10_1101-2021_01_07_425723 186 7 , , , 10_1101-2021_01_07_425723 186 8 and and CC 10_1101-2021_01_07_425723 186 9 tetrahymanol tetrahymanol NN 10_1101-2021_01_07_425723 186 10 contents content NNS 10_1101-2021_01_07_425723 186 11 with with IN 10_1101-2021_01_07_425723 186 12 mean mean JJ 10_1101-2021_01_07_425723 186 13 and and CC 10_1101-2021_01_07_425723 186 14 standard standard JJ 10_1101-2021_01_07_425723 186 15 error error NN 10_1101-2021_01_07_425723 186 16 of of IN 10_1101-2021_01_07_425723 186 17 the the DT 10_1101-2021_01_07_425723 186 18 mean mean NN 10_1101-2021_01_07_425723 186 19 of of IN 10_1101-2021_01_07_425723 186 20 ( ( -LRB- 10_1101-2021_01_07_425723 186 21 left left JJ 10_1101-2021_01_07_425723 186 22 panel panel NN 10_1101-2021_01_07_425723 186 23 ) ) -RRB- 10_1101-2021_01_07_425723 186 24 S. S. NNP 10_1101-2021_01_07_425723 186 25 272 272 CD 10_1101-2021_01_07_425723 186 26 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 186 27 strains strain VBZ 10_1101-2021_01_07_425723 186 28 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 186 29 ( ( -LRB- 10_1101-2021_01_07_425723 186 30 reference reference NN 10_1101-2021_01_07_425723 186 31 ) ) -RRB- 10_1101-2021_01_07_425723 186 32 , , , 10_1101-2021_01_07_425723 186 33 IMX1438 IMX1438 NNP 10_1101-2021_01_07_425723 186 34 ( ( -LRB- 10_1101-2021_01_07_425723 186 35 sga1Δ::TtSTC1 sga1Δ::TtSTC1 NNP 10_1101-2021_01_07_425723 186 36 ) ) -RRB- 10_1101-2021_01_07_425723 186 37 , , , 10_1101-2021_01_07_425723 186 38 and and CC 10_1101-2021_01_07_425723 186 39 K. K. NNP 10_1101-2021_01_07_425723 186 40 marxianus marxianus NN 10_1101-2021_01_07_425723 186 41 strains strain VBZ 10_1101-2021_01_07_425723 186 42 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 186 43 273 273 CD 10_1101-2021_01_07_425723 186 44 ( ( -LRB- 10_1101-2021_01_07_425723 186 45 reference reference NN 10_1101-2021_01_07_425723 186 46 ) ) -RRB- 10_1101-2021_01_07_425723 186 47 , , , 10_1101-2021_01_07_425723 186 48 IMX2323 IMX2323 NNP 10_1101-2021_01_07_425723 186 49 ( ( -LRB- 10_1101-2021_01_07_425723 186 50 TtSTC1 TtSTC1 NNP 10_1101-2021_01_07_425723 186 51 ) ) -RRB- 10_1101-2021_01_07_425723 186 52 . . . 10_1101-2021_01_07_425723 187 1 Lipid lipid NN 10_1101-2021_01_07_425723 187 2 composition composition NN 10_1101-2021_01_07_425723 187 3 of of IN 10_1101-2021_01_07_425723 187 4 single single JJ 10_1101-2021_01_07_425723 187 5 - - HYPH 10_1101-2021_01_07_425723 187 6 cell cell NN 10_1101-2021_01_07_425723 187 7 isolate isolate NN 10_1101-2021_01_07_425723 187 8 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 187 9 ( ( -LRB- 10_1101-2021_01_07_425723 187 10 TtSTC1 TtSTC1 NNP 10_1101-2021_01_07_425723 187 11 ) ) -RRB- 10_1101-2021_01_07_425723 187 12 ( ( -LRB- 10_1101-2021_01_07_425723 187 13 right right JJ 10_1101-2021_01_07_425723 187 14 panel panel NN 10_1101-2021_01_07_425723 187 15 ) ) -RRB- 10_1101-2021_01_07_425723 187 16 274 274 CD 10_1101-2021_01_07_425723 187 17 over over IN 10_1101-2021_01_07_425723 187 18 3 3 CD 10_1101-2021_01_07_425723 187 19 serial serial JJ 10_1101-2021_01_07_425723 187 20 transfers transfer NNS 10_1101-2021_01_07_425723 187 21 ( ( -LRB- 10_1101-2021_01_07_425723 187 22 C1-C3 C1-C3 NNP 10_1101-2021_01_07_425723 187 23 ) ) -RRB- 10_1101-2021_01_07_425723 187 24 . . . 10_1101-2021_01_07_425723 188 1 Data datum NNS 10_1101-2021_01_07_425723 188 2 from from IN 10_1101-2021_01_07_425723 188 3 replicate replicate NN 10_1101-2021_01_07_425723 188 4 cultures culture NNS 10_1101-2021_01_07_425723 188 5 grown grow VBN 10_1101-2021_01_07_425723 188 6 in in IN 10_1101-2021_01_07_425723 188 7 strictly strictly RB 10_1101-2021_01_07_425723 188 8 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 188 9 ( ( -LRB- 10_1101-2021_01_07_425723 188 10 c c NN 10_1101-2021_01_07_425723 188 11 , , , 10_1101-2021_01_07_425723 188 12 f f NNP 10_1101-2021_01_07_425723 188 13 , , , 10_1101-2021_01_07_425723 188 14 g g NNP 10_1101-2021_01_07_425723 188 15 ) ) -RRB- 10_1101-2021_01_07_425723 188 16 or or CC 10_1101-2021_01_07_425723 188 17 275 275 CD 10_1101-2021_01_07_425723 188 18 severely severely RB 10_1101-2021_01_07_425723 188 19 oxygen oxygen NN 10_1101-2021_01_07_425723 188 20 - - HYPH 10_1101-2021_01_07_425723 188 21 limited limit VBN 10_1101-2021_01_07_425723 188 22 shake shake NN 10_1101-2021_01_07_425723 188 23 - - HYPH 10_1101-2021_01_07_425723 188 24 flask flask NN 10_1101-2021_01_07_425723 188 25 cultures culture NNS 10_1101-2021_01_07_425723 188 26 ( ( -LRB- 10_1101-2021_01_07_425723 188 27 d d NN 10_1101-2021_01_07_425723 188 28 , , , 10_1101-2021_01_07_425723 188 29 e e NNP 10_1101-2021_01_07_425723 188 30 ) ) -RRB- 10_1101-2021_01_07_425723 188 31 . . . 10_1101-2021_01_07_425723 189 1 Aerobic Aerobic NNP 10_1101-2021_01_07_425723 189 2 grown grow VBD 10_1101-2021_01_07_425723 189 3 pre pre JJ 10_1101-2021_01_07_425723 189 4 - - NNS 10_1101-2021_01_07_425723 189 5 cultures culture NNS 10_1101-2021_01_07_425723 189 6 were be VBD 10_1101-2021_01_07_425723 189 7 used use VBN 10_1101-2021_01_07_425723 189 8 to to TO 10_1101-2021_01_07_425723 189 9 inoculate inoculate VB 10_1101-2021_01_07_425723 189 10 276 276 CD 10_1101-2021_01_07_425723 189 11 the the DT 10_1101-2021_01_07_425723 189 12 first first JJ 10_1101-2021_01_07_425723 189 13 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 189 14 culture culture NN 10_1101-2021_01_07_425723 189 15 on on IN 10_1101-2021_01_07_425723 189 16 SMG SMG NNP 10_1101-2021_01_07_425723 189 17 - - HYPH 10_1101-2021_01_07_425723 189 18 urea urea NNP 10_1101-2021_01_07_425723 189 19 and and CC 10_1101-2021_01_07_425723 189 20 Tween Tween NNP 10_1101-2021_01_07_425723 189 21 80 80 CD 10_1101-2021_01_07_425723 189 22 , , , 10_1101-2021_01_07_425723 189 23 when when WRB 10_1101-2021_01_07_425723 189 24 the the DT 10_1101-2021_01_07_425723 189 25 optical optical JJ 10_1101-2021_01_07_425723 189 26 density density NN 10_1101-2021_01_07_425723 189 27 started start VBD 10_1101-2021_01_07_425723 189 28 to to TO 10_1101-2021_01_07_425723 189 29 stabilize stabilize VB 10_1101-2021_01_07_425723 189 30 the the DT 10_1101-2021_01_07_425723 189 31 277 277 CD 10_1101-2021_01_07_425723 189 32 cultures culture NNS 10_1101-2021_01_07_425723 189 33 were be VBD 10_1101-2021_01_07_425723 189 34 transferred transfer VBN 10_1101-2021_01_07_425723 189 35 to to IN 10_1101-2021_01_07_425723 189 36 new new JJ 10_1101-2021_01_07_425723 189 37 media medium NNS 10_1101-2021_01_07_425723 189 38 . . . 10_1101-2021_01_07_425723 190 1 Data datum NNS 10_1101-2021_01_07_425723 190 2 depicted depict VBN 10_1101-2021_01_07_425723 190 3 are be VBP 10_1101-2021_01_07_425723 190 4 of of IN 10_1101-2021_01_07_425723 190 5 each each DT 10_1101-2021_01_07_425723 190 6 replicate replicate NN 10_1101-2021_01_07_425723 190 7 culture culture NN 10_1101-2021_01_07_425723 190 8 ( ( -LRB- 10_1101-2021_01_07_425723 190 9 points point NNS 10_1101-2021_01_07_425723 190 10 ) ) -RRB- 10_1101-2021_01_07_425723 190 11 and and CC 10_1101-2021_01_07_425723 190 12 the the DT 10_1101-2021_01_07_425723 190 13 278 278 CD 10_1101-2021_01_07_425723 190 14 .CC .CC , 10_1101-2021_01_07_425723 190 15 - - HYPH 10_1101-2021_01_07_425723 190 16 BY by IN 10_1101-2021_01_07_425723 190 17 - - HYPH 10_1101-2021_01_07_425723 190 18 NC NC NNP 10_1101-2021_01_07_425723 190 19 - - HYPH 10_1101-2021_01_07_425723 190 20 ND ND NNP 10_1101-2021_01_07_425723 190 21 4.0 4.0 CD 10_1101-2021_01_07_425723 190 22 International International NNP 10_1101-2021_01_07_425723 190 23 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 190 24 under under IN 10_1101-2021_01_07_425723 190 25 a a DT 10_1101-2021_01_07_425723 190 26 ( ( -LRB- 10_1101-2021_01_07_425723 190 27 which which WDT 10_1101-2021_01_07_425723 190 28 was be VBD 10_1101-2021_01_07_425723 190 29 not not RB 10_1101-2021_01_07_425723 190 30 certified certify VBN 10_1101-2021_01_07_425723 190 31 by by IN 10_1101-2021_01_07_425723 190 32 peer peer NN 10_1101-2021_01_07_425723 190 33 review review NN 10_1101-2021_01_07_425723 190 34 ) ) -RRB- 10_1101-2021_01_07_425723 190 35 is be VBZ 10_1101-2021_01_07_425723 190 36 the the DT 10_1101-2021_01_07_425723 190 37 author author NN 10_1101-2021_01_07_425723 190 38 / / SYM 10_1101-2021_01_07_425723 190 39 funder funder NN 10_1101-2021_01_07_425723 190 40 , , , 10_1101-2021_01_07_425723 190 41 who who WP 10_1101-2021_01_07_425723 190 42 has have VBZ 10_1101-2021_01_07_425723 190 43 granted grant VBN 10_1101-2021_01_07_425723 190 44 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 190 45 a a DT 10_1101-2021_01_07_425723 190 46 license license NN 10_1101-2021_01_07_425723 190 47 to to TO 10_1101-2021_01_07_425723 190 48 display display VB 10_1101-2021_01_07_425723 190 49 the the DT 10_1101-2021_01_07_425723 190 50 preprint preprint NN 10_1101-2021_01_07_425723 190 51 in in IN 10_1101-2021_01_07_425723 190 52 perpetuity perpetuity NN 10_1101-2021_01_07_425723 190 53 . . . 10_1101-2021_01_07_425723 191 1 It -PRON- PRP 10_1101-2021_01_07_425723 191 2 is be VBZ 10_1101-2021_01_07_425723 191 3 made make VBN 10_1101-2021_01_07_425723 191 4 The the DT 10_1101-2021_01_07_425723 191 5 copyright copyright NN 10_1101-2021_01_07_425723 191 6 holder holder NN 10_1101-2021_01_07_425723 191 7 for for IN 10_1101-2021_01_07_425723 191 8 this this DT 10_1101-2021_01_07_425723 191 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 191 10 version version NN 10_1101-2021_01_07_425723 191 11 posted post VBD 10_1101-2021_01_07_425723 191 12 January January NNP 10_1101-2021_01_07_425723 191 13 8 8 CD 10_1101-2021_01_07_425723 191 14 , , , 10_1101-2021_01_07_425723 191 15 2021 2021 CD 10_1101-2021_01_07_425723 191 16 . . . 10_1101-2021_01_07_425723 191 17 ; ; : 10_1101-2021_01_07_425723 191 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 191 19 : : : 10_1101-2021_01_07_425723 191 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 191 21 preprint preprint NN 10_1101-2021_01_07_425723 191 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 191 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 191 24 18 18 CD 10_1101-2021_01_07_425723 191 25 mean mean NN 10_1101-2021_01_07_425723 191 26 ( ( -LRB- 10_1101-2021_01_07_425723 191 27 dotted dotted JJ 10_1101-2021_01_07_425723 191 28 line line NN 10_1101-2021_01_07_425723 191 29 ) ) -RRB- 10_1101-2021_01_07_425723 191 30 from from IN 10_1101-2021_01_07_425723 191 31 independent independent JJ 10_1101-2021_01_07_425723 191 32 biological biological JJ 10_1101-2021_01_07_425723 191 33 duplicate duplicate JJ 10_1101-2021_01_07_425723 191 34 cultures culture NNS 10_1101-2021_01_07_425723 191 35 , , , 10_1101-2021_01_07_425723 191 36 serial serial JJ 10_1101-2021_01_07_425723 191 37 transfers transfer NNS 10_1101-2021_01_07_425723 191 38 cultures culture NNS 10_1101-2021_01_07_425723 191 39 are be VBP 10_1101-2021_01_07_425723 191 40 279 279 CD 10_1101-2021_01_07_425723 191 41 represented represent VBN 10_1101-2021_01_07_425723 191 42 with with IN 10_1101-2021_01_07_425723 191 43 C1-C5 C1-C5 NNP 10_1101-2021_01_07_425723 191 44 . . . 10_1101-2021_01_07_425723 192 1 Strains Strains NNP 10_1101-2021_01_07_425723 192 2 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 192 3 ( ( -LRB- 10_1101-2021_01_07_425723 192 4 wild wild JJ 10_1101-2021_01_07_425723 192 5 - - HYPH 10_1101-2021_01_07_425723 192 6 type type NN 10_1101-2021_01_07_425723 192 7 , , , 10_1101-2021_01_07_425723 192 8 upward upward RB 10_1101-2021_01_07_425723 192 9 red red JJ 10_1101-2021_01_07_425723 192 10 triangles triangle NNS 10_1101-2021_01_07_425723 192 11 ) ) -RRB- 10_1101-2021_01_07_425723 192 12 , , , 10_1101-2021_01_07_425723 192 13 IMX2323 IMX2323 NNP 10_1101-2021_01_07_425723 192 14 ( ( -LRB- 10_1101-2021_01_07_425723 192 15 TtSTC1 TtSTC1 NNP 10_1101-2021_01_07_425723 192 16 , , , 10_1101-2021_01_07_425723 192 17 cyan cyan NNP 10_1101-2021_01_07_425723 192 18 280 280 CD 10_1101-2021_01_07_425723 192 19 downward downward JJ 10_1101-2021_01_07_425723 192 20 triangle triangle NN 10_1101-2021_01_07_425723 192 21 ) ) -RRB- 10_1101-2021_01_07_425723 192 22 , , , 10_1101-2021_01_07_425723 192 23 and and CC 10_1101-2021_01_07_425723 192 24 the the DT 10_1101-2021_01_07_425723 192 25 single single JJ 10_1101-2021_01_07_425723 192 26 - - HYPH 10_1101-2021_01_07_425723 192 27 cell cell NN 10_1101-2021_01_07_425723 192 28 isolates isolate VBZ 10_1101-2021_01_07_425723 192 29 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 192 30 ( ( -LRB- 10_1101-2021_01_07_425723 192 31 TtSTC1 TtSTC1 NNP 10_1101-2021_01_07_425723 192 32 , , , 10_1101-2021_01_07_425723 192 33 orange orange NNP 10_1101-2021_01_07_425723 192 34 circles circle NNS 10_1101-2021_01_07_425723 192 35 ) ) -RRB- 10_1101-2021_01_07_425723 192 36 , , , 10_1101-2021_01_07_425723 192 37 IMS1131 IMS1131 NNP 10_1101-2021_01_07_425723 192 38 ( ( -LRB- 10_1101-2021_01_07_425723 192 39 TtSTC1 ttstc1 CD 10_1101-2021_01_07_425723 192 40 , , , 10_1101-2021_01_07_425723 192 41 blue blue JJ 10_1101-2021_01_07_425723 192 42 281 281 CD 10_1101-2021_01_07_425723 192 43 circles circle NNS 10_1101-2021_01_07_425723 192 44 ) ) -RRB- 10_1101-2021_01_07_425723 192 45 , , , 10_1101-2021_01_07_425723 192 46 IMS1132 IMS1132 NNP 10_1101-2021_01_07_425723 192 47 ( ( -LRB- 10_1101-2021_01_07_425723 192 48 TtSTC1 ttstc1 CD 10_1101-2021_01_07_425723 192 49 , , , 10_1101-2021_01_07_425723 192 50 yellow yellow JJ 10_1101-2021_01_07_425723 192 51 circles circle NNS 10_1101-2021_01_07_425723 192 52 ) ) -RRB- 10_1101-2021_01_07_425723 192 53 , , , 10_1101-2021_01_07_425723 192 54 IMS1133 IMS1133 NNP 10_1101-2021_01_07_425723 192 55 ( ( -LRB- 10_1101-2021_01_07_425723 192 56 TtSTC1 TtSTC1 NNP 10_1101-2021_01_07_425723 192 57 , , , 10_1101-2021_01_07_425723 192 58 purple purple JJ 10_1101-2021_01_07_425723 192 59 circles circle NNS 10_1101-2021_01_07_425723 192 60 ) ) -RRB- 10_1101-2021_01_07_425723 192 61 . . . 10_1101-2021_01_07_425723 193 1 S. S. NNP 10_1101-2021_01_07_425723 193 2 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 193 3 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 193 4 282 282 CD 10_1101-2021_01_07_425723 193 5 ( ( -LRB- 10_1101-2021_01_07_425723 193 6 reference reference NN 10_1101-2021_01_07_425723 193 7 , , , 10_1101-2021_01_07_425723 193 8 purple purple JJ 10_1101-2021_01_07_425723 193 9 circle circle NN 10_1101-2021_01_07_425723 193 10 ) ) -RRB- 10_1101-2021_01_07_425723 193 11 and and CC 10_1101-2021_01_07_425723 193 12 IMX1438 IMX1438 NNP 10_1101-2021_01_07_425723 193 13 ( ( -LRB- 10_1101-2021_01_07_425723 193 14 TtSTC1 ttstc1 CD 10_1101-2021_01_07_425723 193 15 , , , 10_1101-2021_01_07_425723 193 16 orange orange NNP 10_1101-2021_01_07_425723 193 17 circles circle NNS 10_1101-2021_01_07_425723 193 18 ) ) -RRB- 10_1101-2021_01_07_425723 193 19 . . . 10_1101-2021_01_07_425723 194 1 c c LS 10_1101-2021_01_07_425723 194 2 , , , 10_1101-2021_01_07_425723 194 3 Extended extended JJ 10_1101-2021_01_07_425723 194 4 data datum NNS 10_1101-2021_01_07_425723 194 5 with with IN 10_1101-2021_01_07_425723 194 6 double double JJ 10_1101-2021_01_07_425723 194 7 inoculum inoculum NNP 10_1101-2021_01_07_425723 194 8 283 283 CD 10_1101-2021_01_07_425723 194 9 size size NN 10_1101-2021_01_07_425723 194 10 is be VBZ 10_1101-2021_01_07_425723 194 11 available available JJ 10_1101-2021_01_07_425723 194 12 in in IN 10_1101-2021_01_07_425723 194 13 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 194 14 Fig Fig NNP 10_1101-2021_01_07_425723 194 15 . . . 10_1101-2021_01_07_425723 195 1 10 10 CD 10_1101-2021_01_07_425723 195 2 . . . 10_1101-2021_01_07_425723 195 3 d d NNP 10_1101-2021_01_07_425723 195 4 , , , 10_1101-2021_01_07_425723 195 5 Extended extended JJ 10_1101-2021_01_07_425723 195 6 data data NN 10_1101-2021_01_07_425723 195 7 is be VBZ 10_1101-2021_01_07_425723 195 8 available available JJ 10_1101-2021_01_07_425723 195 9 in in IN 10_1101-2021_01_07_425723 195 10 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 195 11 Fig Fig NNP 10_1101-2021_01_07_425723 195 12 . . . 10_1101-2021_01_07_425723 196 1 9a 9a UH 10_1101-2021_01_07_425723 196 2 . . . 10_1101-2021_01_07_425723 197 1 284 284 CD 10_1101-2021_01_07_425723 197 2 Test test NN 10_1101-2021_01_07_425723 197 3 of of IN 10_1101-2021_01_07_425723 197 4 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 197 5 thermotolerance thermotolerance NN 10_1101-2021_01_07_425723 197 6 and and CC 10_1101-2021_01_07_425723 197 7 selection selection NN 10_1101-2021_01_07_425723 197 8 for for IN 10_1101-2021_01_07_425723 197 9 fast fast JJ 10_1101-2021_01_07_425723 197 10 growing grow VBG 10_1101-2021_01_07_425723 197 11 anaerobes anaerobe NNS 10_1101-2021_01_07_425723 197 12 285 285 CD 10_1101-2021_01_07_425723 197 13 One one CD 10_1101-2021_01_07_425723 197 14 of of IN 10_1101-2021_01_07_425723 197 15 the the DT 10_1101-2021_01_07_425723 197 16 attractive attractive JJ 10_1101-2021_01_07_425723 197 17 phenotypes phenotype NNS 10_1101-2021_01_07_425723 197 18 of of IN 10_1101-2021_01_07_425723 197 19 K. K. NNP 10_1101-2021_01_07_425723 197 20 marxianus marxianus NN 10_1101-2021_01_07_425723 197 21 for for IN 10_1101-2021_01_07_425723 197 22 industrial industrial JJ 10_1101-2021_01_07_425723 197 23 application application NN 10_1101-2021_01_07_425723 197 24 is be VBZ 10_1101-2021_01_07_425723 197 25 its -PRON- PRP$ 10_1101-2021_01_07_425723 197 26 high high JJ 10_1101-2021_01_07_425723 197 27 thermotolerance thermotolerance NN 10_1101-2021_01_07_425723 197 28 286 286 CD 10_1101-2021_01_07_425723 197 29 with with IN 10_1101-2021_01_07_425723 197 30 reported report VBN 10_1101-2021_01_07_425723 197 31 maximum maximum JJ 10_1101-2021_01_07_425723 197 32 growth growth NN 10_1101-2021_01_07_425723 197 33 temperatures temperature NNS 10_1101-2021_01_07_425723 197 34 of of IN 10_1101-2021_01_07_425723 197 35 46 46 CD 10_1101-2021_01_07_425723 197 36 - - SYM 10_1101-2021_01_07_425723 197 37 52 52 CD 10_1101-2021_01_07_425723 197 38 ° ° , 10_1101-2021_01_07_425723 197 39 C49,50 C49,50 NNP 10_1101-2021_01_07_425723 197 40 . . . 10_1101-2021_01_07_425723 198 1 To to TO 10_1101-2021_01_07_425723 198 2 test test VB 10_1101-2021_01_07_425723 198 3 if if IN 10_1101-2021_01_07_425723 198 4 anaerobically anaerobically RB 10_1101-2021_01_07_425723 198 5 growing grow VBG 10_1101-2021_01_07_425723 198 6 287 287 CD 10_1101-2021_01_07_425723 198 7 tetrahymanol tetrahymanol NN 10_1101-2021_01_07_425723 198 8 - - HYPH 10_1101-2021_01_07_425723 198 9 producing produce VBG 10_1101-2021_01_07_425723 198 10 strains strain NNS 10_1101-2021_01_07_425723 198 11 retained retain VBN 10_1101-2021_01_07_425723 198 12 thermotolerance thermotolerance NN 10_1101-2021_01_07_425723 198 13 , , , 10_1101-2021_01_07_425723 198 14 strain strain VB 10_1101-2021_01_07_425723 198 15 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 198 16 was be VBD 10_1101-2021_01_07_425723 198 17 grown grow VBN 10_1101-2021_01_07_425723 198 18 in in IN 10_1101-2021_01_07_425723 198 19 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 198 20 288 288 CD 10_1101-2021_01_07_425723 198 21 sequential sequential JJ 10_1101-2021_01_07_425723 198 22 - - HYPH 10_1101-2021_01_07_425723 198 23 batch batch NN 10_1101-2021_01_07_425723 198 24 - - HYPH 10_1101-2021_01_07_425723 198 25 reactor reactor NN 10_1101-2021_01_07_425723 198 26 ( ( -LRB- 10_1101-2021_01_07_425723 198 27 SBR SBR NNP 10_1101-2021_01_07_425723 198 28 ) ) -RRB- 10_1101-2021_01_07_425723 198 29 cultures culture NNS 10_1101-2021_01_07_425723 198 30 ( ( -LRB- 10_1101-2021_01_07_425723 198 31 Fig fig NN 10_1101-2021_01_07_425723 198 32 . . . 10_1101-2021_01_07_425723 199 1 6 6 LS 10_1101-2021_01_07_425723 199 2 ) ) -RRB- 10_1101-2021_01_07_425723 199 3 in in IN 10_1101-2021_01_07_425723 199 4 which which WDT 10_1101-2021_01_07_425723 199 5 , , , 10_1101-2021_01_07_425723 199 6 after after IN 10_1101-2021_01_07_425723 199 7 an an DT 10_1101-2021_01_07_425723 199 8 initial initial JJ 10_1101-2021_01_07_425723 199 9 growth growth NN 10_1101-2021_01_07_425723 199 10 cycle cycle NN 10_1101-2021_01_07_425723 199 11 at at IN 10_1101-2021_01_07_425723 199 12 30 30 CD 10_1101-2021_01_07_425723 199 13 ° ° , 10_1101-2021_01_07_425723 199 14 C C NNP 10_1101-2021_01_07_425723 199 15 , , , 10_1101-2021_01_07_425723 199 16 the the DT 10_1101-2021_01_07_425723 199 17 growth growth NN 10_1101-2021_01_07_425723 199 18 289 289 CD 10_1101-2021_01_07_425723 199 19 temperature temperature NN 10_1101-2021_01_07_425723 199 20 was be VBD 10_1101-2021_01_07_425723 199 21 shifted shift VBN 10_1101-2021_01_07_425723 199 22 to to IN 10_1101-2021_01_07_425723 199 23 42 42 CD 10_1101-2021_01_07_425723 199 24 ° ° NNS 10_1101-2021_01_07_425723 199 25 C C NNP 10_1101-2021_01_07_425723 199 26 . . . 10_1101-2021_01_07_425723 200 1 Specific specific JJ 10_1101-2021_01_07_425723 200 2 growth growth NN 10_1101-2021_01_07_425723 200 3 at at IN 10_1101-2021_01_07_425723 200 4 42 42 CD 10_1101-2021_01_07_425723 200 5 ° ° NNS 10_1101-2021_01_07_425723 200 6 C C NNP 10_1101-2021_01_07_425723 200 7 progressively progressively RB 10_1101-2021_01_07_425723 200 8 accelerated accelerate VBD 10_1101-2021_01_07_425723 200 9 from from IN 10_1101-2021_01_07_425723 200 10 0.06 0.06 CD 10_1101-2021_01_07_425723 200 11 h-1 h-1 NNP 10_1101-2021_01_07_425723 200 12 to to IN 10_1101-2021_01_07_425723 200 13 290 290 CD 10_1101-2021_01_07_425723 200 14 0.13 0.13 CD 10_1101-2021_01_07_425723 200 15 h-1 h-1 NN 10_1101-2021_01_07_425723 200 16 over over IN 10_1101-2021_01_07_425723 200 17 17 17 CD 10_1101-2021_01_07_425723 200 18 SBR SBR NNP 10_1101-2021_01_07_425723 200 19 cycles cycle NNS 10_1101-2021_01_07_425723 200 20 ( ( -LRB- 10_1101-2021_01_07_425723 200 21 corresponding correspond VBG 10_1101-2021_01_07_425723 200 22 to to IN 10_1101-2021_01_07_425723 200 23 ca ca NNP 10_1101-2021_01_07_425723 200 24 . . . 10_1101-2021_01_07_425723 201 1 290 290 CD 10_1101-2021_01_07_425723 201 2 generations generation NNS 10_1101-2021_01_07_425723 201 3 ; ; : 10_1101-2021_01_07_425723 201 4 Fig Fig NNP 10_1101-2021_01_07_425723 201 5 . . . 10_1101-2021_01_07_425723 202 1 6b 6b LS 10_1101-2021_01_07_425723 202 2 ) ) -RRB- 10_1101-2021_01_07_425723 202 3 . . . 10_1101-2021_01_07_425723 203 1 A a DT 10_1101-2021_01_07_425723 203 2 subsequent subsequent JJ 10_1101-2021_01_07_425723 203 3 temperature temperature NN 10_1101-2021_01_07_425723 203 4 291 291 CD 10_1101-2021_01_07_425723 203 5 increase increase NN 10_1101-2021_01_07_425723 203 6 to to IN 10_1101-2021_01_07_425723 203 7 45 45 CD 10_1101-2021_01_07_425723 203 8 ° ° , 10_1101-2021_01_07_425723 203 9 C C NNP 10_1101-2021_01_07_425723 203 10 led lead VBD 10_1101-2021_01_07_425723 203 11 to to IN 10_1101-2021_01_07_425723 203 12 a a DT 10_1101-2021_01_07_425723 203 13 strong strong JJ 10_1101-2021_01_07_425723 203 14 decrease decrease NN 10_1101-2021_01_07_425723 203 15 of of IN 10_1101-2021_01_07_425723 203 16 the the DT 10_1101-2021_01_07_425723 203 17 specific specific JJ 10_1101-2021_01_07_425723 203 18 growth growth NN 10_1101-2021_01_07_425723 203 19 rate rate NN 10_1101-2021_01_07_425723 203 20 which which WDT 10_1101-2021_01_07_425723 203 21 , , , 10_1101-2021_01_07_425723 203 22 after after IN 10_1101-2021_01_07_425723 203 23 approximately approximately RB 10_1101-2021_01_07_425723 203 24 1000 1000 CD 10_1101-2021_01_07_425723 203 25 292 292 CD 10_1101-2021_01_07_425723 203 26 generations generation NNS 10_1101-2021_01_07_425723 203 27 of of IN 10_1101-2021_01_07_425723 203 28 selective selective JJ 10_1101-2021_01_07_425723 203 29 growth growth NN 10_1101-2021_01_07_425723 203 30 , , , 10_1101-2021_01_07_425723 203 31 stabilized stabilize VBD 10_1101-2021_01_07_425723 203 32 at at IN 10_1101-2021_01_07_425723 203 33 approximately approximately RB 10_1101-2021_01_07_425723 203 34 0.08 0.08 CD 10_1101-2021_01_07_425723 203 35 h-1 h-1 NNP 10_1101-2021_01_07_425723 203 36 . . . 10_1101-2021_01_07_425723 204 1 Whole whole JJ 10_1101-2021_01_07_425723 204 2 - - HYPH 10_1101-2021_01_07_425723 204 3 population population NN 10_1101-2021_01_07_425723 204 4 genome genome NN 10_1101-2021_01_07_425723 204 5 293 293 CD 10_1101-2021_01_07_425723 204 6 sequencing sequencing NN 10_1101-2021_01_07_425723 204 7 of of IN 10_1101-2021_01_07_425723 204 8 the the DT 10_1101-2021_01_07_425723 204 9 evolved evolved JJ 10_1101-2021_01_07_425723 204 10 populations population NNS 10_1101-2021_01_07_425723 204 11 revealed reveal VBD 10_1101-2021_01_07_425723 204 12 no no DT 10_1101-2021_01_07_425723 204 13 common common JJ 10_1101-2021_01_07_425723 204 14 mutations mutation NNS 10_1101-2021_01_07_425723 204 15 or or CC 10_1101-2021_01_07_425723 204 16 chromosomal chromosomal NN 10_1101-2021_01_07_425723 204 17 copy copy NN 10_1101-2021_01_07_425723 204 18 number number NN 10_1101-2021_01_07_425723 204 19 294 294 CD 10_1101-2021_01_07_425723 204 20 variations variation NNS 10_1101-2021_01_07_425723 204 21 ( ( -LRB- 10_1101-2021_01_07_425723 204 22 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 204 23 Table Table NNP 10_1101-2021_01_07_425723 204 24 1 1 CD 10_1101-2021_01_07_425723 204 25 ) ) -RRB- 10_1101-2021_01_07_425723 204 26 . . . 10_1101-2021_01_07_425723 205 1 These these DT 10_1101-2021_01_07_425723 205 2 data datum NNS 10_1101-2021_01_07_425723 205 3 show show VBP 10_1101-2021_01_07_425723 205 4 that that IN 10_1101-2021_01_07_425723 205 5 TtSTC1-expressing ttstc1-expresse VBG 10_1101-2021_01_07_425723 205 6 K. K. NNP 10_1101-2021_01_07_425723 205 7 marxianus marxianus NN 10_1101-2021_01_07_425723 205 8 can can MD 10_1101-2021_01_07_425723 205 9 grow grow VB 10_1101-2021_01_07_425723 205 10 295 295 CD 10_1101-2021_01_07_425723 205 11 anaerobically anaerobically RB 10_1101-2021_01_07_425723 205 12 at at IN 10_1101-2021_01_07_425723 205 13 temperatures temperature NNS 10_1101-2021_01_07_425723 205 14 up up RB 10_1101-2021_01_07_425723 205 15 to to IN 10_1101-2021_01_07_425723 205 16 at at RB 10_1101-2021_01_07_425723 205 17 least least RBS 10_1101-2021_01_07_425723 205 18 45 45 CD 10_1101-2021_01_07_425723 205 19 ° ° , 10_1101-2021_01_07_425723 205 20 C C NNP 10_1101-2021_01_07_425723 205 21 . . . 10_1101-2021_01_07_425723 206 1 296 296 CD 10_1101-2021_01_07_425723 206 2 .CC .CC : 10_1101-2021_01_07_425723 206 3 - - HYPH 10_1101-2021_01_07_425723 206 4 BY by IN 10_1101-2021_01_07_425723 206 5 - - HYPH 10_1101-2021_01_07_425723 206 6 NC NC NNP 10_1101-2021_01_07_425723 206 7 - - HYPH 10_1101-2021_01_07_425723 206 8 ND ND NNP 10_1101-2021_01_07_425723 206 9 4.0 4.0 CD 10_1101-2021_01_07_425723 206 10 International International NNP 10_1101-2021_01_07_425723 206 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 206 12 under under IN 10_1101-2021_01_07_425723 206 13 a a DT 10_1101-2021_01_07_425723 206 14 ( ( -LRB- 10_1101-2021_01_07_425723 206 15 which which WDT 10_1101-2021_01_07_425723 206 16 was be VBD 10_1101-2021_01_07_425723 206 17 not not RB 10_1101-2021_01_07_425723 206 18 certified certify VBN 10_1101-2021_01_07_425723 206 19 by by IN 10_1101-2021_01_07_425723 206 20 peer peer NN 10_1101-2021_01_07_425723 206 21 review review NN 10_1101-2021_01_07_425723 206 22 ) ) -RRB- 10_1101-2021_01_07_425723 206 23 is be VBZ 10_1101-2021_01_07_425723 206 24 the the DT 10_1101-2021_01_07_425723 206 25 author author NN 10_1101-2021_01_07_425723 206 26 / / SYM 10_1101-2021_01_07_425723 206 27 funder funder NN 10_1101-2021_01_07_425723 206 28 , , , 10_1101-2021_01_07_425723 206 29 who who WP 10_1101-2021_01_07_425723 206 30 has have VBZ 10_1101-2021_01_07_425723 206 31 granted grant VBN 10_1101-2021_01_07_425723 206 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 206 33 a a DT 10_1101-2021_01_07_425723 206 34 license license NN 10_1101-2021_01_07_425723 206 35 to to TO 10_1101-2021_01_07_425723 206 36 display display VB 10_1101-2021_01_07_425723 206 37 the the DT 10_1101-2021_01_07_425723 206 38 preprint preprint NN 10_1101-2021_01_07_425723 206 39 in in IN 10_1101-2021_01_07_425723 206 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 206 41 . . . 10_1101-2021_01_07_425723 207 1 It -PRON- PRP 10_1101-2021_01_07_425723 207 2 is be VBZ 10_1101-2021_01_07_425723 207 3 made make VBN 10_1101-2021_01_07_425723 207 4 The the DT 10_1101-2021_01_07_425723 207 5 copyright copyright NN 10_1101-2021_01_07_425723 207 6 holder holder NN 10_1101-2021_01_07_425723 207 7 for for IN 10_1101-2021_01_07_425723 207 8 this this DT 10_1101-2021_01_07_425723 207 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 207 10 version version NN 10_1101-2021_01_07_425723 207 11 posted post VBD 10_1101-2021_01_07_425723 207 12 January January NNP 10_1101-2021_01_07_425723 207 13 8 8 CD 10_1101-2021_01_07_425723 207 14 , , , 10_1101-2021_01_07_425723 207 15 2021 2021 CD 10_1101-2021_01_07_425723 207 16 . . . 10_1101-2021_01_07_425723 207 17 ; ; : 10_1101-2021_01_07_425723 207 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 207 19 : : : 10_1101-2021_01_07_425723 207 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 207 21 preprint preprint NN 10_1101-2021_01_07_425723 207 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 207 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ RB 10_1101-2021_01_07_425723 207 24 19 19 CD 10_1101-2021_01_07_425723 207 25 Fig fig NN 10_1101-2021_01_07_425723 207 26 . . . 10_1101-2021_01_07_425723 208 1 6 6 CD 10_1101-2021_01_07_425723 208 2 | | NNS 10_1101-2021_01_07_425723 208 3 Thermotolerance thermotolerance NN 10_1101-2021_01_07_425723 208 4 and and CC 10_1101-2021_01_07_425723 208 5 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 208 6 growth growth NN 10_1101-2021_01_07_425723 208 7 of of IN 10_1101-2021_01_07_425723 208 8 tetrahymanol tetrahymanol NN 10_1101-2021_01_07_425723 208 9 - - HYPH 10_1101-2021_01_07_425723 208 10 producing produce VBG 10_1101-2021_01_07_425723 208 11 K. K. NNP 10_1101-2021_01_07_425723 208 12 marxianus marxianus NN 10_1101-2021_01_07_425723 208 13 strain strain NN 10_1101-2021_01_07_425723 208 14 . . . 10_1101-2021_01_07_425723 209 1 The the DT 10_1101-2021_01_07_425723 209 2 297 297 CD 10_1101-2021_01_07_425723 209 3 strain strain NN 10_1101-2021_01_07_425723 209 4 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 209 5 was be VBD 10_1101-2021_01_07_425723 209 6 grown grow VBN 10_1101-2021_01_07_425723 209 7 in in IN 10_1101-2021_01_07_425723 209 8 triplicate triplicate NN 10_1101-2021_01_07_425723 209 9 sequential sequential JJ 10_1101-2021_01_07_425723 209 10 batch batch NN 10_1101-2021_01_07_425723 209 11 bioreactor bioreactor NN 10_1101-2021_01_07_425723 209 12 cultivations cultivation NNS 10_1101-2021_01_07_425723 209 13 in in IN 10_1101-2021_01_07_425723 209 14 synthetic synthetic JJ 10_1101-2021_01_07_425723 209 15 media medium NNS 10_1101-2021_01_07_425723 209 16 298 298 CD 10_1101-2021_01_07_425723 209 17 supplemented supplement VBD 10_1101-2021_01_07_425723 209 18 with with IN 10_1101-2021_01_07_425723 209 19 20 20 CD 10_1101-2021_01_07_425723 209 20 g·L-1 g·L-1 NNP 10_1101-2021_01_07_425723 209 21 glucose glucose NN 10_1101-2021_01_07_425723 209 22 and and CC 10_1101-2021_01_07_425723 209 23 420 420 CD 10_1101-2021_01_07_425723 209 24 mg·L-1 mg·L-1 NNP 10_1101-2021_01_07_425723 209 25 Tween Tween NNP 10_1101-2021_01_07_425723 209 26 80 80 CD 10_1101-2021_01_07_425723 209 27 at at IN 10_1101-2021_01_07_425723 209 28 pH pH NNP 10_1101-2021_01_07_425723 209 29 5.0 5.0 CD 10_1101-2021_01_07_425723 209 30 . . . 10_1101-2021_01_07_425723 209 31 a a DT 10_1101-2021_01_07_425723 209 32 , , , 10_1101-2021_01_07_425723 209 33 Experimental experimental JJ 10_1101-2021_01_07_425723 209 34 design design NN 10_1101-2021_01_07_425723 209 35 of of IN 10_1101-2021_01_07_425723 209 36 299 299 CD 10_1101-2021_01_07_425723 209 37 sequential sequential JJ 10_1101-2021_01_07_425723 209 38 batch batch NN 10_1101-2021_01_07_425723 209 39 fermentation fermentation NN 10_1101-2021_01_07_425723 209 40 with with IN 10_1101-2021_01_07_425723 209 41 cycles cycle NNS 10_1101-2021_01_07_425723 209 42 at at IN 10_1101-2021_01_07_425723 209 43 step step NN 10_1101-2021_01_07_425723 209 44 - - HYPH 10_1101-2021_01_07_425723 209 45 wise wise JJ 10_1101-2021_01_07_425723 209 46 increasing increase VBG 10_1101-2021_01_07_425723 209 47 temperatures temperature NNS 10_1101-2021_01_07_425723 209 48 to to TO 10_1101-2021_01_07_425723 209 49 select select VB 10_1101-2021_01_07_425723 209 50 for for IN 10_1101-2021_01_07_425723 209 51 faster fast JJR 10_1101-2021_01_07_425723 209 52 300 300 CD 10_1101-2021_01_07_425723 209 53 growing grow VBG 10_1101-2021_01_07_425723 209 54 mutants mutant NNS 10_1101-2021_01_07_425723 209 55 , , , 10_1101-2021_01_07_425723 209 56 each each DT 10_1101-2021_01_07_425723 209 57 cycle cycle NN 10_1101-2021_01_07_425723 209 58 consisted consist VBD 10_1101-2021_01_07_425723 209 59 of of IN 10_1101-2021_01_07_425723 209 60 three three CD 10_1101-2021_01_07_425723 209 61 phases phase NNS 10_1101-2021_01_07_425723 209 62 ; ; : 10_1101-2021_01_07_425723 209 63 ( ( -LRB- 10_1101-2021_01_07_425723 209 64 i i NN 10_1101-2021_01_07_425723 209 65 ) ) -RRB- 10_1101-2021_01_07_425723 209 66 ( ( -LRB- 10_1101-2021_01_07_425723 209 67 re)filling re)fille VBG 10_1101-2021_01_07_425723 209 68 of of IN 10_1101-2021_01_07_425723 209 69 the the DT 10_1101-2021_01_07_425723 209 70 bioreactor bioreactor NN 10_1101-2021_01_07_425723 209 71 with with IN 10_1101-2021_01_07_425723 209 72 fresh fresh JJ 10_1101-2021_01_07_425723 209 73 media medium NNS 10_1101-2021_01_07_425723 209 74 301 301 CD 10_1101-2021_01_07_425723 209 75 up up IN 10_1101-2021_01_07_425723 209 76 to to TO 10_1101-2021_01_07_425723 209 77 100 100 CD 10_1101-2021_01_07_425723 209 78 mL ml NN 10_1101-2021_01_07_425723 209 79 and and CC 10_1101-2021_01_07_425723 209 80 adjustment adjustment NN 10_1101-2021_01_07_425723 209 81 of of IN 10_1101-2021_01_07_425723 209 82 temperature temperature NN 10_1101-2021_01_07_425723 209 83 to to IN 10_1101-2021_01_07_425723 209 84 a a DT 10_1101-2021_01_07_425723 209 85 new new JJ 10_1101-2021_01_07_425723 209 86 set set NN 10_1101-2021_01_07_425723 209 87 - - HYPH 10_1101-2021_01_07_425723 209 88 point point NN 10_1101-2021_01_07_425723 209 89 , , , 10_1101-2021_01_07_425723 209 90 ( ( -LRB- 10_1101-2021_01_07_425723 209 91 ii ii NNP 10_1101-2021_01_07_425723 209 92 ) ) -RRB- 10_1101-2021_01_07_425723 209 93 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 209 94 batch batch NNP 10_1101-2021_01_07_425723 209 95 fermentation fermentation NN 10_1101-2021_01_07_425723 209 96 at at IN 10_1101-2021_01_07_425723 209 97 a a DT 10_1101-2021_01_07_425723 209 98 302 302 CD 10_1101-2021_01_07_425723 209 99 fixed fix VBN 10_1101-2021_01_07_425723 209 100 culture culture NN 10_1101-2021_01_07_425723 209 101 temperature temperature NN 10_1101-2021_01_07_425723 209 102 with with IN 10_1101-2021_01_07_425723 209 103 continuous continuous JJ 10_1101-2021_01_07_425723 209 104 N2 N2 NNP 10_1101-2021_01_07_425723 209 105 sparging sparge VBG 10_1101-2021_01_07_425723 209 106 for for IN 10_1101-2021_01_07_425723 209 107 monitoring monitoring NN 10_1101-2021_01_07_425723 209 108 of of IN 10_1101-2021_01_07_425723 209 109 CO2 CO2 NNP 10_1101-2021_01_07_425723 209 110 in in IN 10_1101-2021_01_07_425723 209 111 the the DT 10_1101-2021_01_07_425723 209 112 culture culture NN 10_1101-2021_01_07_425723 209 113 off off IN 10_1101-2021_01_07_425723 209 114 - - HYPH 10_1101-2021_01_07_425723 209 115 gas gas NN 10_1101-2021_01_07_425723 209 116 , , , 10_1101-2021_01_07_425723 209 117 and and CC 10_1101-2021_01_07_425723 209 118 303 303 CD 10_1101-2021_01_07_425723 209 119 ( ( -LRB- 10_1101-2021_01_07_425723 209 120 iii iii NNP 10_1101-2021_01_07_425723 209 121 ) ) -RRB- 10_1101-2021_01_07_425723 209 122 fast fast JJ 10_1101-2021_01_07_425723 209 123 broth broth NN 10_1101-2021_01_07_425723 209 124 withdrawal withdrawal NN 10_1101-2021_01_07_425723 209 125 leaving leave VBG 10_1101-2021_01_07_425723 209 126 7 7 CD 10_1101-2021_01_07_425723 209 127 mL mL NNP 10_1101-2021_01_07_425723 209 128 ( ( -LRB- 10_1101-2021_01_07_425723 209 129 14.3 14.3 CD 10_1101-2021_01_07_425723 209 130 fold fold JJ 10_1101-2021_01_07_425723 209 131 dilution dilution NN 10_1101-2021_01_07_425723 209 132 ) ) -RRB- 10_1101-2021_01_07_425723 209 133 to to TO 10_1101-2021_01_07_425723 209 134 inoculate inoculate VB 10_1101-2021_01_07_425723 209 135 the the DT 10_1101-2021_01_07_425723 209 136 next next JJ 10_1101-2021_01_07_425723 209 137 batch batch NN 10_1101-2021_01_07_425723 209 138 . . . 10_1101-2021_01_07_425723 210 1 b b LS 10_1101-2021_01_07_425723 210 2 , , , 10_1101-2021_01_07_425723 210 3 Maximum Maximum NNP 10_1101-2021_01_07_425723 210 4 304 304 CD 10_1101-2021_01_07_425723 210 5 specific specific JJ 10_1101-2021_01_07_425723 210 6 estimated estimate VBN 10_1101-2021_01_07_425723 210 7 growth growth NN 10_1101-2021_01_07_425723 210 8 rate rate NN 10_1101-2021_01_07_425723 210 9 ( ( -LRB- 10_1101-2021_01_07_425723 210 10 circles circle NNS 10_1101-2021_01_07_425723 210 11 ) ) -RRB- 10_1101-2021_01_07_425723 210 12 of of IN 10_1101-2021_01_07_425723 210 13 each each DT 10_1101-2021_01_07_425723 210 14 batch batch NN 10_1101-2021_01_07_425723 210 15 cycle cycle NN 10_1101-2021_01_07_425723 210 16 for for IN 10_1101-2021_01_07_425723 210 17 the the DT 10_1101-2021_01_07_425723 210 18 three three CD 10_1101-2021_01_07_425723 210 19 independent independent JJ 10_1101-2021_01_07_425723 210 20 bioreactor bioreactor NNP 10_1101-2021_01_07_425723 210 21 305 305 CD 10_1101-2021_01_07_425723 210 22 cultivations cultivation NNS 10_1101-2021_01_07_425723 210 23 ( ( -LRB- 10_1101-2021_01_07_425723 210 24 M3R M3R NNP 10_1101-2021_01_07_425723 210 25 blue blue NNP 10_1101-2021_01_07_425723 210 26 , , , 10_1101-2021_01_07_425723 210 27 M5R M5R NNP 10_1101-2021_01_07_425723 210 28 orange orange NNP 10_1101-2021_01_07_425723 210 29 , , , 10_1101-2021_01_07_425723 210 30 M6L M6L NNP 10_1101-2021_01_07_425723 210 31 grey grey NNP 10_1101-2021_01_07_425723 210 32 ) ) -RRB- 10_1101-2021_01_07_425723 210 33 with with IN 10_1101-2021_01_07_425723 210 34 the the DT 10_1101-2021_01_07_425723 210 35 estimated estimate VBN 10_1101-2021_01_07_425723 210 36 number number NN 10_1101-2021_01_07_425723 210 37 of of IN 10_1101-2021_01_07_425723 210 38 generations generation NNS 10_1101-2021_01_07_425723 210 39 . . . 10_1101-2021_01_07_425723 211 1 The the DT 10_1101-2021_01_07_425723 211 2 growth growth NN 10_1101-2021_01_07_425723 211 3 306 306 CD 10_1101-2021_01_07_425723 211 4 rate rate NN 10_1101-2021_01_07_425723 211 5 was be VBD 10_1101-2021_01_07_425723 211 6 calculated calculate VBN 10_1101-2021_01_07_425723 211 7 from from IN 10_1101-2021_01_07_425723 211 8 the the DT 10_1101-2021_01_07_425723 211 9 CO2 CO2 NNP 10_1101-2021_01_07_425723 211 10 production production NN 10_1101-2021_01_07_425723 211 11 as as IN 10_1101-2021_01_07_425723 211 12 measured measure VBN 10_1101-2021_01_07_425723 211 13 in in IN 10_1101-2021_01_07_425723 211 14 the the DT 10_1101-2021_01_07_425723 211 15 off off JJ 10_1101-2021_01_07_425723 211 16 - - HYPH 10_1101-2021_01_07_425723 211 17 gas gas NN 10_1101-2021_01_07_425723 211 18 and and CC 10_1101-2021_01_07_425723 211 19 should should MD 10_1101-2021_01_07_425723 211 20 be be VB 10_1101-2021_01_07_425723 211 21 interpreted interpret VBN 10_1101-2021_01_07_425723 211 22 as as IN 10_1101-2021_01_07_425723 211 23 an an DT 10_1101-2021_01_07_425723 211 24 307 307 CD 10_1101-2021_01_07_425723 211 25 estimate estimate NN 10_1101-2021_01_07_425723 211 26 and and CC 10_1101-2021_01_07_425723 211 27 in in IN 10_1101-2021_01_07_425723 211 28 some some DT 10_1101-2021_01_07_425723 211 29 cases case NNS 10_1101-2021_01_07_425723 211 30 could could MD 10_1101-2021_01_07_425723 211 31 not not RB 10_1101-2021_01_07_425723 211 32 be be VB 10_1101-2021_01_07_425723 211 33 calculated calculate VBN 10_1101-2021_01_07_425723 211 34 . . . 10_1101-2021_01_07_425723 212 1 The the DT 10_1101-2021_01_07_425723 212 2 culture culture NN 10_1101-2021_01_07_425723 212 3 temperature temperature NN 10_1101-2021_01_07_425723 212 4 profile profile NN 10_1101-2021_01_07_425723 212 5 ( ( -LRB- 10_1101-2021_01_07_425723 212 6 dotted dotted JJ 10_1101-2021_01_07_425723 212 7 line line NN 10_1101-2021_01_07_425723 212 8 ) ) -RRB- 10_1101-2021_01_07_425723 212 9 for for IN 10_1101-2021_01_07_425723 212 10 308 308 CD 10_1101-2021_01_07_425723 212 11 .CC .CC : 10_1101-2021_01_07_425723 212 12 - - HYPH 10_1101-2021_01_07_425723 212 13 BY by IN 10_1101-2021_01_07_425723 212 14 - - HYPH 10_1101-2021_01_07_425723 212 15 NC NC NNP 10_1101-2021_01_07_425723 212 16 - - HYPH 10_1101-2021_01_07_425723 212 17 ND ND NNP 10_1101-2021_01_07_425723 212 18 4.0 4.0 CD 10_1101-2021_01_07_425723 212 19 International International NNP 10_1101-2021_01_07_425723 212 20 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 212 21 under under IN 10_1101-2021_01_07_425723 212 22 a a DT 10_1101-2021_01_07_425723 212 23 ( ( -LRB- 10_1101-2021_01_07_425723 212 24 which which WDT 10_1101-2021_01_07_425723 212 25 was be VBD 10_1101-2021_01_07_425723 212 26 not not RB 10_1101-2021_01_07_425723 212 27 certified certify VBN 10_1101-2021_01_07_425723 212 28 by by IN 10_1101-2021_01_07_425723 212 29 peer peer NN 10_1101-2021_01_07_425723 212 30 review review NN 10_1101-2021_01_07_425723 212 31 ) ) -RRB- 10_1101-2021_01_07_425723 212 32 is be VBZ 10_1101-2021_01_07_425723 212 33 the the DT 10_1101-2021_01_07_425723 212 34 author author NN 10_1101-2021_01_07_425723 212 35 / / SYM 10_1101-2021_01_07_425723 212 36 funder funder NN 10_1101-2021_01_07_425723 212 37 , , , 10_1101-2021_01_07_425723 212 38 who who WP 10_1101-2021_01_07_425723 212 39 has have VBZ 10_1101-2021_01_07_425723 212 40 granted grant VBN 10_1101-2021_01_07_425723 212 41 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 212 42 a a DT 10_1101-2021_01_07_425723 212 43 license license NN 10_1101-2021_01_07_425723 212 44 to to TO 10_1101-2021_01_07_425723 212 45 display display VB 10_1101-2021_01_07_425723 212 46 the the DT 10_1101-2021_01_07_425723 212 47 preprint preprint NN 10_1101-2021_01_07_425723 212 48 in in IN 10_1101-2021_01_07_425723 212 49 perpetuity perpetuity NN 10_1101-2021_01_07_425723 212 50 . . . 10_1101-2021_01_07_425723 213 1 It -PRON- PRP 10_1101-2021_01_07_425723 213 2 is be VBZ 10_1101-2021_01_07_425723 213 3 made make VBN 10_1101-2021_01_07_425723 213 4 The the DT 10_1101-2021_01_07_425723 213 5 copyright copyright NN 10_1101-2021_01_07_425723 213 6 holder holder NN 10_1101-2021_01_07_425723 213 7 for for IN 10_1101-2021_01_07_425723 213 8 this this DT 10_1101-2021_01_07_425723 213 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 213 10 version version NN 10_1101-2021_01_07_425723 213 11 posted post VBD 10_1101-2021_01_07_425723 213 12 January January NNP 10_1101-2021_01_07_425723 213 13 8 8 CD 10_1101-2021_01_07_425723 213 14 , , , 10_1101-2021_01_07_425723 213 15 2021 2021 CD 10_1101-2021_01_07_425723 213 16 . . . 10_1101-2021_01_07_425723 213 17 ; ; : 10_1101-2021_01_07_425723 213 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 213 19 : : : 10_1101-2021_01_07_425723 213 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 213 21 preprint preprint NN 10_1101-2021_01_07_425723 213 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 213 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CC 10_1101-2021_01_07_425723 213 24 20 20 CD 10_1101-2021_01_07_425723 213 25 each each DT 10_1101-2021_01_07_425723 213 26 independent independent JJ 10_1101-2021_01_07_425723 213 27 bioreactor bioreactor NN 10_1101-2021_01_07_425723 213 28 cultivation cultivation NN 10_1101-2021_01_07_425723 213 29 ( ( -LRB- 10_1101-2021_01_07_425723 213 30 blue blue JJ 10_1101-2021_01_07_425723 213 31 , , , 10_1101-2021_01_07_425723 213 32 grey grey NNP 10_1101-2021_01_07_425723 213 33 , , , 10_1101-2021_01_07_425723 213 34 orange orange NNP 10_1101-2021_01_07_425723 213 35 ) ) -RRB- 10_1101-2021_01_07_425723 213 36 consisted consist VBD 10_1101-2021_01_07_425723 213 37 of of IN 10_1101-2021_01_07_425723 213 38 a a DT 10_1101-2021_01_07_425723 213 39 step step NN 10_1101-2021_01_07_425723 213 40 - - HYPH 10_1101-2021_01_07_425723 213 41 wise wise JJ 10_1101-2021_01_07_425723 213 42 increment increment NN 10_1101-2021_01_07_425723 213 43 of of IN 10_1101-2021_01_07_425723 213 44 the the DT 10_1101-2021_01_07_425723 213 45 309 309 CD 10_1101-2021_01_07_425723 213 46 temperature temperature NN 10_1101-2021_01_07_425723 213 47 at at IN 10_1101-2021_01_07_425723 213 48 the the DT 10_1101-2021_01_07_425723 213 49 onset onset NN 10_1101-2021_01_07_425723 213 50 of of IN 10_1101-2021_01_07_425723 213 51 the the DT 10_1101-2021_01_07_425723 213 52 fermentation fermentation NN 10_1101-2021_01_07_425723 213 53 phase phase NN 10_1101-2021_01_07_425723 213 54 in in IN 10_1101-2021_01_07_425723 213 55 each each DT 10_1101-2021_01_07_425723 213 56 batch batch NN 10_1101-2021_01_07_425723 213 57 cycle cycle NN 10_1101-2021_01_07_425723 213 58 . . . 10_1101-2021_01_07_425723 214 1 c c LS 10_1101-2021_01_07_425723 214 2 , , , 10_1101-2021_01_07_425723 214 3 Representative Representative NNP 10_1101-2021_01_07_425723 214 4 section section NN 10_1101-2021_01_07_425723 214 5 of of IN 10_1101-2021_01_07_425723 214 6 310 310 CD 10_1101-2021_01_07_425723 214 7 CO2 CO2 NNP 10_1101-2021_01_07_425723 214 8 off off IN 10_1101-2021_01_07_425723 214 9 - - HYPH 10_1101-2021_01_07_425723 214 10 gas gas NN 10_1101-2021_01_07_425723 214 11 profiles profile NNS 10_1101-2021_01_07_425723 214 12 of of IN 10_1101-2021_01_07_425723 214 13 the the DT 10_1101-2021_01_07_425723 214 14 individual individual JJ 10_1101-2021_01_07_425723 214 15 bioreactor bioreactor NN 10_1101-2021_01_07_425723 214 16 ( ( -LRB- 10_1101-2021_01_07_425723 214 17 M5R m5r NN 10_1101-2021_01_07_425723 214 18 ) ) -RRB- 10_1101-2021_01_07_425723 214 19 cultivation cultivation NN 10_1101-2021_01_07_425723 214 20 over over IN 10_1101-2021_01_07_425723 214 21 time time NN 10_1101-2021_01_07_425723 214 22 with with IN 10_1101-2021_01_07_425723 214 23 CO2 CO2 NNP 10_1101-2021_01_07_425723 214 24 fraction fraction NN 10_1101-2021_01_07_425723 214 25 ( ( -LRB- 10_1101-2021_01_07_425723 214 26 orange orange NNP 10_1101-2021_01_07_425723 214 27 311 311 CD 10_1101-2021_01_07_425723 214 28 line line NN 10_1101-2021_01_07_425723 214 29 ) ) -RRB- 10_1101-2021_01_07_425723 214 30 and and CC 10_1101-2021_01_07_425723 214 31 culture culture NN 10_1101-2021_01_07_425723 214 32 temperature temperature NN 10_1101-2021_01_07_425723 214 33 ( ( -LRB- 10_1101-2021_01_07_425723 214 34 grey grey JJ 10_1101-2021_01_07_425723 214 35 dotted dotted JJ 10_1101-2021_01_07_425723 214 36 line line NN 10_1101-2021_01_07_425723 214 37 ) ) -RRB- 10_1101-2021_01_07_425723 214 38 , , , 10_1101-2021_01_07_425723 214 39 data datum NNS 10_1101-2021_01_07_425723 214 40 of of IN 10_1101-2021_01_07_425723 214 41 the the DT 10_1101-2021_01_07_425723 214 42 entire entire JJ 10_1101-2021_01_07_425723 214 43 experiment experiment NN 10_1101-2021_01_07_425723 214 44 is be VBZ 10_1101-2021_01_07_425723 214 45 available available JJ 10_1101-2021_01_07_425723 214 46 in in IN 10_1101-2021_01_07_425723 214 47 312 312 CD 10_1101-2021_01_07_425723 214 48 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 214 49 Fig Fig NNP 10_1101-2021_01_07_425723 214 50 . . . 10_1101-2021_01_07_425723 215 1 11 11 CD 10_1101-2021_01_07_425723 215 2 ( ( -LRB- 10_1101-2021_01_07_425723 215 3 Data datum NNS 10_1101-2021_01_07_425723 215 4 availability availability NN 10_1101-2021_01_07_425723 215 5 ) ) -RRB- 10_1101-2021_01_07_425723 215 6 . . . 10_1101-2021_01_07_425723 216 1 313 313 CD 10_1101-2021_01_07_425723 216 2 Discussion discussion NN 10_1101-2021_01_07_425723 216 3 314 314 CD 10_1101-2021_01_07_425723 216 4 Industrial industrial JJ 10_1101-2021_01_07_425723 216 5 production production NN 10_1101-2021_01_07_425723 216 6 of of IN 10_1101-2021_01_07_425723 216 7 ethanol ethanol NN 10_1101-2021_01_07_425723 216 8 from from IN 10_1101-2021_01_07_425723 216 9 carbohydrates carbohydrate NNS 10_1101-2021_01_07_425723 216 10 relies rely VBZ 10_1101-2021_01_07_425723 216 11 on on IN 10_1101-2021_01_07_425723 216 12 S. S. NNP 10_1101-2021_01_07_425723 216 13 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 216 14 , , , 10_1101-2021_01_07_425723 216 15 due due IN 10_1101-2021_01_07_425723 216 16 to to IN 10_1101-2021_01_07_425723 216 17 its -PRON- PRP$ 10_1101-2021_01_07_425723 216 18 capacity capacity NN 10_1101-2021_01_07_425723 216 19 for for IN 10_1101-2021_01_07_425723 216 20 315 315 CD 10_1101-2021_01_07_425723 216 21 efficient efficient JJ 10_1101-2021_01_07_425723 216 22 , , , 10_1101-2021_01_07_425723 216 23 fast fast JJ 10_1101-2021_01_07_425723 216 24 alcoholic alcoholic JJ 10_1101-2021_01_07_425723 216 25 fermentation fermentation NN 10_1101-2021_01_07_425723 216 26 and and CC 10_1101-2021_01_07_425723 216 27 growth growth NN 10_1101-2021_01_07_425723 216 28 under under IN 10_1101-2021_01_07_425723 216 29 strictly strictly RB 10_1101-2021_01_07_425723 216 30 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 216 31 process process NN 10_1101-2021_01_07_425723 216 32 conditions condition NNS 10_1101-2021_01_07_425723 216 33 . . . 10_1101-2021_01_07_425723 217 1 Many many JJ 10_1101-2021_01_07_425723 217 2 316 316 CD 10_1101-2021_01_07_425723 217 3 facultatively facultatively RB 10_1101-2021_01_07_425723 217 4 fermentative fermentative JJ 10_1101-2021_01_07_425723 217 5 yeast yeast NN 10_1101-2021_01_07_425723 217 6 species specie NNS 10_1101-2021_01_07_425723 217 7 outside outside IN 10_1101-2021_01_07_425723 217 8 the the DT 10_1101-2021_01_07_425723 217 9 Saccharomycotina Saccharomycotina NNP 10_1101-2021_01_07_425723 217 10 WGD WGD NNP 10_1101-2021_01_07_425723 217 11 - - HYPH 10_1101-2021_01_07_425723 217 12 clade clade NNP 10_1101-2021_01_07_425723 217 13 also also RB 10_1101-2021_01_07_425723 217 14 rapidly rapidly RB 10_1101-2021_01_07_425723 217 15 ferment ferment VB 10_1101-2021_01_07_425723 217 16 317 317 CD 10_1101-2021_01_07_425723 217 17 sugars sugar NNS 10_1101-2021_01_07_425723 217 18 to to IN 10_1101-2021_01_07_425723 217 19 ethanol ethanol NN 10_1101-2021_01_07_425723 217 20 under under IN 10_1101-2021_01_07_425723 217 21 oxygen oxygen NN 10_1101-2021_01_07_425723 217 22 - - HYPH 10_1101-2021_01_07_425723 217 23 limited limit VBN 10_1101-2021_01_07_425723 217 24 conditions26 conditions26 NNS 10_1101-2021_01_07_425723 217 25 , , , 10_1101-2021_01_07_425723 217 26 but but CC 10_1101-2021_01_07_425723 217 27 can can MD 10_1101-2021_01_07_425723 217 28 not not RB 10_1101-2021_01_07_425723 217 29 grow grow VB 10_1101-2021_01_07_425723 217 30 and and CC 10_1101-2021_01_07_425723 217 31 ferment ferment VB 10_1101-2021_01_07_425723 217 32 in in IN 10_1101-2021_01_07_425723 217 33 the the DT 10_1101-2021_01_07_425723 217 34 complete complete JJ 10_1101-2021_01_07_425723 217 35 318 318 CD 10_1101-2021_01_07_425723 217 36 absence absence NN 10_1101-2021_01_07_425723 217 37 of of IN 10_1101-2021_01_07_425723 217 38 oxygen11,13,25 oxygen11,13,25 NNP 10_1101-2021_01_07_425723 217 39 . . . 10_1101-2021_01_07_425723 218 1 Identifying identify VBG 10_1101-2021_01_07_425723 218 2 and and CC 10_1101-2021_01_07_425723 218 3 eliminating eliminate VBG 10_1101-2021_01_07_425723 218 4 oxygen oxygen NN 10_1101-2021_01_07_425723 218 5 requirements requirement NNS 10_1101-2021_01_07_425723 218 6 of of IN 10_1101-2021_01_07_425723 218 7 these these DT 10_1101-2021_01_07_425723 218 8 yeasts yeast NNS 10_1101-2021_01_07_425723 218 9 is be VBZ 10_1101-2021_01_07_425723 218 10 essential essential JJ 10_1101-2021_01_07_425723 218 11 to to TO 10_1101-2021_01_07_425723 218 12 319 319 CD 10_1101-2021_01_07_425723 218 13 unlock unlock VB 10_1101-2021_01_07_425723 218 14 their -PRON- PRP$ 10_1101-2021_01_07_425723 218 15 industrially industrially RB 10_1101-2021_01_07_425723 218 16 relevant relevant JJ 10_1101-2021_01_07_425723 218 17 traits trait NNS 10_1101-2021_01_07_425723 218 18 for for IN 10_1101-2021_01_07_425723 218 19 application application NN 10_1101-2021_01_07_425723 218 20 . . . 10_1101-2021_01_07_425723 219 1 Here here RB 10_1101-2021_01_07_425723 219 2 , , , 10_1101-2021_01_07_425723 219 3 this this DT 10_1101-2021_01_07_425723 219 4 challenge challenge NN 10_1101-2021_01_07_425723 219 5 was be VBD 10_1101-2021_01_07_425723 219 6 addressed address VBN 10_1101-2021_01_07_425723 219 7 for for IN 10_1101-2021_01_07_425723 219 8 the the DT 10_1101-2021_01_07_425723 219 9 320 320 CD 10_1101-2021_01_07_425723 219 10 thermotolerant thermotolerant NN 10_1101-2021_01_07_425723 219 11 yeast yeast NN 10_1101-2021_01_07_425723 219 12 K. K. NNP 10_1101-2021_01_07_425723 219 13 marxianus marxianus NN 10_1101-2021_01_07_425723 219 14 , , , 10_1101-2021_01_07_425723 219 15 using use VBG 10_1101-2021_01_07_425723 219 16 a a DT 10_1101-2021_01_07_425723 219 17 systematic systematic JJ 10_1101-2021_01_07_425723 219 18 approach approach NN 10_1101-2021_01_07_425723 219 19 based base VBN 10_1101-2021_01_07_425723 219 20 on on IN 10_1101-2021_01_07_425723 219 21 chemostat chemostat NNP 10_1101-2021_01_07_425723 219 22 - - HYPH 10_1101-2021_01_07_425723 219 23 based base VBN 10_1101-2021_01_07_425723 219 24 quantitative quantitative JJ 10_1101-2021_01_07_425723 219 25 321 321 CD 10_1101-2021_01_07_425723 219 26 physiology physiology NN 10_1101-2021_01_07_425723 219 27 , , , 10_1101-2021_01_07_425723 219 28 genome genome JJ 10_1101-2021_01_07_425723 219 29 and and CC 10_1101-2021_01_07_425723 219 30 transcriptome transcriptome DT 10_1101-2021_01_07_425723 219 31 analysis analysis NN 10_1101-2021_01_07_425723 219 32 , , , 10_1101-2021_01_07_425723 219 33 sterol sterol NN 10_1101-2021_01_07_425723 219 34 - - HYPH 10_1101-2021_01_07_425723 219 35 uptake uptake NN 10_1101-2021_01_07_425723 219 36 assays assay NNS 10_1101-2021_01_07_425723 219 37 and and CC 10_1101-2021_01_07_425723 219 38 genetic genetic JJ 10_1101-2021_01_07_425723 219 39 modification modification NN 10_1101-2021_01_07_425723 219 40 . . . 10_1101-2021_01_07_425723 220 1 S. S. NNP 10_1101-2021_01_07_425723 220 2 322 322 CD 10_1101-2021_01_07_425723 220 3 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 220 4 , , , 10_1101-2021_01_07_425723 220 5 which which WDT 10_1101-2021_01_07_425723 220 6 was be VBD 10_1101-2021_01_07_425723 220 7 used use VBN 10_1101-2021_01_07_425723 220 8 as as IN 10_1101-2021_01_07_425723 220 9 a a DT 10_1101-2021_01_07_425723 220 10 reference reference NN 10_1101-2021_01_07_425723 220 11 in in IN 10_1101-2021_01_07_425723 220 12 this this DT 10_1101-2021_01_07_425723 220 13 study study NN 10_1101-2021_01_07_425723 220 14 , , , 10_1101-2021_01_07_425723 220 15 shows show VBZ 10_1101-2021_01_07_425723 220 16 strongly strongly RB 10_1101-2021_01_07_425723 220 17 different different JJ 10_1101-2021_01_07_425723 220 18 genome genome NN 10_1101-2021_01_07_425723 220 19 - - HYPH 10_1101-2021_01_07_425723 220 20 wide wide JJ 10_1101-2021_01_07_425723 220 21 323 323 CD 10_1101-2021_01_07_425723 220 22 expression expression NN 10_1101-2021_01_07_425723 220 23 profiles profile NNS 10_1101-2021_01_07_425723 220 24 under under IN 10_1101-2021_01_07_425723 220 25 aerobic aerobic JJ 10_1101-2021_01_07_425723 220 26 and and CC 10_1101-2021_01_07_425723 220 27 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 220 28 or or CC 10_1101-2021_01_07_425723 220 29 oxygen oxygen NN 10_1101-2021_01_07_425723 220 30 - - HYPH 10_1101-2021_01_07_425723 220 31 limited limit VBN 10_1101-2021_01_07_425723 220 32 conditions51 conditions51 NN 10_1101-2021_01_07_425723 220 33 . . . 10_1101-2021_01_07_425723 221 1 Although although IN 10_1101-2021_01_07_425723 221 2 only only RB 10_1101-2021_01_07_425723 221 3 a a DT 10_1101-2021_01_07_425723 221 4 small small JJ 10_1101-2021_01_07_425723 221 5 324 324 CD 10_1101-2021_01_07_425723 221 6 fraction fraction NN 10_1101-2021_01_07_425723 221 7 of of IN 10_1101-2021_01_07_425723 221 8 these these DT 10_1101-2021_01_07_425723 221 9 differences difference NNS 10_1101-2021_01_07_425723 221 10 were be VBD 10_1101-2021_01_07_425723 221 11 conserved conserve VBN 10_1101-2021_01_07_425723 221 12 in in IN 10_1101-2021_01_07_425723 221 13 K. K. NNP 10_1101-2021_01_07_425723 221 14 marxianus marxianus NN 10_1101-2021_01_07_425723 221 15 ( ( -LRB- 10_1101-2021_01_07_425723 221 16 Fig fig NN 10_1101-2021_01_07_425723 221 17 . . . 10_1101-2021_01_07_425723 222 1 2 2 LS 10_1101-2021_01_07_425723 222 2 ) ) -RRB- 10_1101-2021_01_07_425723 222 3 , , , 10_1101-2021_01_07_425723 222 4 we -PRON- PRP 10_1101-2021_01_07_425723 222 5 were be VBD 10_1101-2021_01_07_425723 222 6 able able JJ 10_1101-2021_01_07_425723 222 7 to to TO 10_1101-2021_01_07_425723 222 8 identify identify VB 10_1101-2021_01_07_425723 222 9 absence absence NN 10_1101-2021_01_07_425723 222 10 325 325 CD 10_1101-2021_01_07_425723 222 11 of of IN 10_1101-2021_01_07_425723 222 12 a a DT 10_1101-2021_01_07_425723 222 13 functional functional JJ 10_1101-2021_01_07_425723 222 14 sterol sterol JJ 10_1101-2021_01_07_425723 222 15 import import NN 10_1101-2021_01_07_425723 222 16 system system NN 10_1101-2021_01_07_425723 222 17 as as IN 10_1101-2021_01_07_425723 222 18 the the DT 10_1101-2021_01_07_425723 222 19 critical critical JJ 10_1101-2021_01_07_425723 222 20 cause cause NN 10_1101-2021_01_07_425723 222 21 for for IN 10_1101-2021_01_07_425723 222 22 its -PRON- PRP$ 10_1101-2021_01_07_425723 222 23 inability inability NN 10_1101-2021_01_07_425723 222 24 to to TO 10_1101-2021_01_07_425723 222 25 grow grow VB 10_1101-2021_01_07_425723 222 26 anaerobically anaerobically RB 10_1101-2021_01_07_425723 222 27 . . . 10_1101-2021_01_07_425723 223 1 Enabling enable VBG 10_1101-2021_01_07_425723 223 2 326 326 CD 10_1101-2021_01_07_425723 223 3 synthesis synthesis NN 10_1101-2021_01_07_425723 223 4 of of IN 10_1101-2021_01_07_425723 223 5 the the DT 10_1101-2021_01_07_425723 223 6 sterol sterol JJ 10_1101-2021_01_07_425723 223 7 surrogate surrogate JJ 10_1101-2021_01_07_425723 223 8 tetrahymanol tetrahymanol NNS 10_1101-2021_01_07_425723 223 9 yielded yield VBD 10_1101-2021_01_07_425723 223 10 strains strain NNS 10_1101-2021_01_07_425723 223 11 that that WDT 10_1101-2021_01_07_425723 223 12 grew grow VBD 10_1101-2021_01_07_425723 223 13 anaerobically anaerobically RB 10_1101-2021_01_07_425723 223 14 at at IN 10_1101-2021_01_07_425723 223 15 temperatures temperature NNS 10_1101-2021_01_07_425723 223 16 327 327 CD 10_1101-2021_01_07_425723 223 17 above above IN 10_1101-2021_01_07_425723 223 18 the the DT 10_1101-2021_01_07_425723 223 19 permissive permissive JJ 10_1101-2021_01_07_425723 223 20 temperature temperature NN 10_1101-2021_01_07_425723 223 21 range range NN 10_1101-2021_01_07_425723 223 22 of of IN 10_1101-2021_01_07_425723 223 23 S. S. NNP 10_1101-2021_01_07_425723 223 24 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 223 25 . . . 10_1101-2021_01_07_425723 224 1 328 328 CD 10_1101-2021_01_07_425723 224 2 A a DT 10_1101-2021_01_07_425723 224 3 short short JJ 10_1101-2021_01_07_425723 224 4 adaptation adaptation NN 10_1101-2021_01_07_425723 224 5 phase phase NN 10_1101-2021_01_07_425723 224 6 of of IN 10_1101-2021_01_07_425723 224 7 tetrahymanol tetrahymanol NN 10_1101-2021_01_07_425723 224 8 - - HYPH 10_1101-2021_01_07_425723 224 9 producing produce VBG 10_1101-2021_01_07_425723 224 10 K. K. NNP 10_1101-2021_01_07_425723 224 11 marxianus marxianus NN 10_1101-2021_01_07_425723 224 12 strains strain VBZ 10_1101-2021_01_07_425723 224 13 under under IN 10_1101-2021_01_07_425723 224 14 oxygen oxygen NN 10_1101-2021_01_07_425723 224 15 - - HYPH 10_1101-2021_01_07_425723 224 16 limited limit VBN 10_1101-2021_01_07_425723 224 17 329 329 CD 10_1101-2021_01_07_425723 224 18 conditions condition NNS 10_1101-2021_01_07_425723 224 19 reproducibly reproducibly RB 10_1101-2021_01_07_425723 224 20 enabled enable VBD 10_1101-2021_01_07_425723 224 21 strictly strictly RB 10_1101-2021_01_07_425723 224 22 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 224 23 growth growth NN 10_1101-2021_01_07_425723 224 24 . . . 10_1101-2021_01_07_425723 225 1 Although although IN 10_1101-2021_01_07_425723 225 2 this this DT 10_1101-2021_01_07_425723 225 3 ability ability NN 10_1101-2021_01_07_425723 225 4 was be VBD 10_1101-2021_01_07_425723 225 5 retained retain VBN 10_1101-2021_01_07_425723 225 6 after after IN 10_1101-2021_01_07_425723 225 7 330 330 CD 10_1101-2021_01_07_425723 225 8 aerobic aerobic JJ 10_1101-2021_01_07_425723 225 9 isolation isolation NN 10_1101-2021_01_07_425723 225 10 of of IN 10_1101-2021_01_07_425723 225 11 single single JJ 10_1101-2021_01_07_425723 225 12 - - HYPH 10_1101-2021_01_07_425723 225 13 cell cell NN 10_1101-2021_01_07_425723 225 14 lines line NNS 10_1101-2021_01_07_425723 225 15 , , , 10_1101-2021_01_07_425723 225 16 we -PRON- PRP 10_1101-2021_01_07_425723 225 17 were be VBD 10_1101-2021_01_07_425723 225 18 unable unable JJ 10_1101-2021_01_07_425723 225 19 to to TO 10_1101-2021_01_07_425723 225 20 attribute attribute VB 10_1101-2021_01_07_425723 225 21 this this DT 10_1101-2021_01_07_425723 225 22 adaptation adaptation NN 10_1101-2021_01_07_425723 225 23 to to IN 10_1101-2021_01_07_425723 225 24 mutations mutation NNS 10_1101-2021_01_07_425723 225 25 . . . 10_1101-2021_01_07_425723 226 1 In in IN 10_1101-2021_01_07_425723 226 2 331 331 CD 10_1101-2021_01_07_425723 226 3 .CC .CC , 10_1101-2021_01_07_425723 226 4 - - HYPH 10_1101-2021_01_07_425723 226 5 BY by IN 10_1101-2021_01_07_425723 226 6 - - HYPH 10_1101-2021_01_07_425723 226 7 NC NC NNP 10_1101-2021_01_07_425723 226 8 - - HYPH 10_1101-2021_01_07_425723 226 9 ND ND NNP 10_1101-2021_01_07_425723 226 10 4.0 4.0 CD 10_1101-2021_01_07_425723 226 11 International International NNP 10_1101-2021_01_07_425723 226 12 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 226 13 under under IN 10_1101-2021_01_07_425723 226 14 a a DT 10_1101-2021_01_07_425723 226 15 ( ( -LRB- 10_1101-2021_01_07_425723 226 16 which which WDT 10_1101-2021_01_07_425723 226 17 was be VBD 10_1101-2021_01_07_425723 226 18 not not RB 10_1101-2021_01_07_425723 226 19 certified certify VBN 10_1101-2021_01_07_425723 226 20 by by IN 10_1101-2021_01_07_425723 226 21 peer peer NN 10_1101-2021_01_07_425723 226 22 review review NN 10_1101-2021_01_07_425723 226 23 ) ) -RRB- 10_1101-2021_01_07_425723 226 24 is be VBZ 10_1101-2021_01_07_425723 226 25 the the DT 10_1101-2021_01_07_425723 226 26 author author NN 10_1101-2021_01_07_425723 226 27 / / SYM 10_1101-2021_01_07_425723 226 28 funder funder NN 10_1101-2021_01_07_425723 226 29 , , , 10_1101-2021_01_07_425723 226 30 who who WP 10_1101-2021_01_07_425723 226 31 has have VBZ 10_1101-2021_01_07_425723 226 32 granted grant VBN 10_1101-2021_01_07_425723 226 33 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 226 34 a a DT 10_1101-2021_01_07_425723 226 35 license license NN 10_1101-2021_01_07_425723 226 36 to to TO 10_1101-2021_01_07_425723 226 37 display display VB 10_1101-2021_01_07_425723 226 38 the the DT 10_1101-2021_01_07_425723 226 39 preprint preprint NN 10_1101-2021_01_07_425723 226 40 in in IN 10_1101-2021_01_07_425723 226 41 perpetuity perpetuity NN 10_1101-2021_01_07_425723 226 42 . . . 10_1101-2021_01_07_425723 227 1 It -PRON- PRP 10_1101-2021_01_07_425723 227 2 is be VBZ 10_1101-2021_01_07_425723 227 3 made make VBN 10_1101-2021_01_07_425723 227 4 The the DT 10_1101-2021_01_07_425723 227 5 copyright copyright NN 10_1101-2021_01_07_425723 227 6 holder holder NN 10_1101-2021_01_07_425723 227 7 for for IN 10_1101-2021_01_07_425723 227 8 this this DT 10_1101-2021_01_07_425723 227 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 227 10 version version NN 10_1101-2021_01_07_425723 227 11 posted post VBD 10_1101-2021_01_07_425723 227 12 January January NNP 10_1101-2021_01_07_425723 227 13 8 8 CD 10_1101-2021_01_07_425723 227 14 , , , 10_1101-2021_01_07_425723 227 15 2021 2021 CD 10_1101-2021_01_07_425723 227 16 . . . 10_1101-2021_01_07_425723 227 17 ; ; : 10_1101-2021_01_07_425723 227 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 227 19 : : : 10_1101-2021_01_07_425723 227 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 227 21 preprint preprint NN 10_1101-2021_01_07_425723 227 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 227 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ RB 10_1101-2021_01_07_425723 227 24 21 21 CD 10_1101-2021_01_07_425723 227 25 contrast contrast NN 10_1101-2021_01_07_425723 227 26 to to IN 10_1101-2021_01_07_425723 227 27 wild wild JJ 10_1101-2021_01_07_425723 227 28 - - HYPH 10_1101-2021_01_07_425723 227 29 type type NN 10_1101-2021_01_07_425723 227 30 K. K. NNP 10_1101-2021_01_07_425723 227 31 marxianus marxianus NN 10_1101-2021_01_07_425723 227 32 , , , 10_1101-2021_01_07_425723 227 33 a a DT 10_1101-2021_01_07_425723 227 34 non non JJ 10_1101-2021_01_07_425723 227 35 - - JJ 10_1101-2021_01_07_425723 227 36 adapted adapted JJ 10_1101-2021_01_07_425723 227 37 tetrahymanol tetrahymanol NN 10_1101-2021_01_07_425723 227 38 - - HYPH 10_1101-2021_01_07_425723 227 39 producing produce VBG 10_1101-2021_01_07_425723 227 40 strain strain NN 10_1101-2021_01_07_425723 227 41 did do VBD 10_1101-2021_01_07_425723 227 42 not not RB 10_1101-2021_01_07_425723 227 43 show show VB 10_1101-2021_01_07_425723 227 44 ‘ ' `` 10_1101-2021_01_07_425723 227 45 carry-332 carry-332 NN 10_1101-2021_01_07_425723 227 46 over over IN 10_1101-2021_01_07_425723 227 47 growth growth NN 10_1101-2021_01_07_425723 227 48 ’ ' '' 10_1101-2021_01_07_425723 227 49 after after IN 10_1101-2021_01_07_425723 227 50 transfer transfer NN 10_1101-2021_01_07_425723 227 51 from from IN 10_1101-2021_01_07_425723 227 52 aerobic aerobic JJ 10_1101-2021_01_07_425723 227 53 to to TO 10_1101-2021_01_07_425723 227 54 strictly strictly RB 10_1101-2021_01_07_425723 227 55 anaerobic anaerobic VB 10_1101-2021_01_07_425723 227 56 conditions condition NNS 10_1101-2021_01_07_425723 227 57 and and CC 10_1101-2021_01_07_425723 227 58 adapted adapt VBN 10_1101-2021_01_07_425723 227 59 cultures culture NNS 10_1101-2021_01_07_425723 227 60 showed show VBD 10_1101-2021_01_07_425723 227 61 333 333 CD 10_1101-2021_01_07_425723 227 62 reduced reduced JJ 10_1101-2021_01_07_425723 227 63 squalene squalene NN 10_1101-2021_01_07_425723 227 64 contents content NNS 10_1101-2021_01_07_425723 227 65 ( ( -LRB- 10_1101-2021_01_07_425723 227 66 Fig fig NN 10_1101-2021_01_07_425723 227 67 . . . 10_1101-2021_01_07_425723 228 1 5 5 LS 10_1101-2021_01_07_425723 228 2 ) ) -RRB- 10_1101-2021_01_07_425723 228 3 . . . 10_1101-2021_01_07_425723 229 1 These these DT 10_1101-2021_01_07_425723 229 2 observations observation NNS 10_1101-2021_01_07_425723 229 3 suggest suggest VBP 10_1101-2021_01_07_425723 229 4 that that IN 10_1101-2021_01_07_425723 229 5 interactions interaction NNS 10_1101-2021_01_07_425723 229 6 between between IN 10_1101-2021_01_07_425723 229 7 tetrahymanol tetrahymanol NNS 10_1101-2021_01_07_425723 229 8 , , , 10_1101-2021_01_07_425723 229 9 334 334 CD 10_1101-2021_01_07_425723 229 10 ergosterol ergosterol JJ 10_1101-2021_01_07_425723 229 11 and/or and/or CC 10_1101-2021_01_07_425723 229 12 squalene squalene VB 10_1101-2021_01_07_425723 229 13 influence influence NN 10_1101-2021_01_07_425723 229 14 the the DT 10_1101-2021_01_07_425723 229 15 onset onset NN 10_1101-2021_01_07_425723 229 16 of of IN 10_1101-2021_01_07_425723 229 17 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 229 18 growth growth NN 10_1101-2021_01_07_425723 229 19 and and CC 10_1101-2021_01_07_425723 229 20 that that IN 10_1101-2021_01_07_425723 229 21 oxygen oxygen NN 10_1101-2021_01_07_425723 229 22 - - HYPH 10_1101-2021_01_07_425723 229 23 limited limit VBN 10_1101-2021_01_07_425723 229 24 growth growth NN 10_1101-2021_01_07_425723 229 25 335 335 CD 10_1101-2021_01_07_425723 229 26 results result NNS 10_1101-2021_01_07_425723 229 27 in in IN 10_1101-2021_01_07_425723 229 28 a a DT 10_1101-2021_01_07_425723 229 29 stable stable JJ 10_1101-2021_01_07_425723 229 30 balance balance NN 10_1101-2021_01_07_425723 229 31 between between IN 10_1101-2021_01_07_425723 229 32 these these DT 10_1101-2021_01_07_425723 229 33 lipids lipid NNS 10_1101-2021_01_07_425723 229 34 that that WDT 10_1101-2021_01_07_425723 229 35 is be VBZ 10_1101-2021_01_07_425723 229 36 permissive permissive JJ 10_1101-2021_01_07_425723 229 37 for for IN 10_1101-2021_01_07_425723 229 38 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 229 39 growth growth NN 10_1101-2021_01_07_425723 229 40 . . . 10_1101-2021_01_07_425723 230 1 336 336 CD 10_1101-2021_01_07_425723 230 2 Comparative comparative JJ 10_1101-2021_01_07_425723 230 3 genomic genomic JJ 10_1101-2021_01_07_425723 230 4 studies study NNS 10_1101-2021_01_07_425723 230 5 in in IN 10_1101-2021_01_07_425723 230 6 Saccharomycotina Saccharomycotina NNP 10_1101-2021_01_07_425723 230 7 yeasts yeast NNS 10_1101-2021_01_07_425723 230 8 have have VBP 10_1101-2021_01_07_425723 230 9 previously previously RB 10_1101-2021_01_07_425723 230 10 led lead VBN 10_1101-2021_01_07_425723 230 11 to to IN 10_1101-2021_01_07_425723 230 12 the the DT 10_1101-2021_01_07_425723 230 13 hypothesis hypothesis NN 10_1101-2021_01_07_425723 230 14 that that IN 10_1101-2021_01_07_425723 230 15 337 337 CD 10_1101-2021_01_07_425723 230 16 sterol sterol JJ 10_1101-2021_01_07_425723 230 17 transporters transporter NNS 10_1101-2021_01_07_425723 230 18 are be VBP 10_1101-2021_01_07_425723 230 19 absent absent JJ 10_1101-2021_01_07_425723 230 20 from from IN 10_1101-2021_01_07_425723 230 21 pre pre JJ 10_1101-2021_01_07_425723 230 22 - - JJ 10_1101-2021_01_07_425723 230 23 WGD wgd JJ 10_1101-2021_01_07_425723 230 24 yeast yeast NN 10_1101-2021_01_07_425723 230 25 species11,52 species11,52 RB 10_1101-2021_01_07_425723 230 26 . . . 10_1101-2021_01_07_425723 231 1 While while IN 10_1101-2021_01_07_425723 231 2 our -PRON- PRP$ 10_1101-2021_01_07_425723 231 3 observations observation NNS 10_1101-2021_01_07_425723 231 4 on on IN 10_1101-2021_01_07_425723 231 5 K. K. NNP 10_1101-2021_01_07_425723 231 6 marxianus marxianus NNP 10_1101-2021_01_07_425723 231 7 338 338 CD 10_1101-2021_01_07_425723 231 8 reinforce reinforce NN 10_1101-2021_01_07_425723 231 9 this this DT 10_1101-2021_01_07_425723 231 10 hypothesis hypothesis NN 10_1101-2021_01_07_425723 231 11 , , , 10_1101-2021_01_07_425723 231 12 which which WDT 10_1101-2021_01_07_425723 231 13 was be VBD 10_1101-2021_01_07_425723 231 14 hitherto hitherto VBN 10_1101-2021_01_07_425723 231 15 not not RB 10_1101-2021_01_07_425723 231 16 experimentally experimentally RB 10_1101-2021_01_07_425723 231 17 tested test VBN 10_1101-2021_01_07_425723 231 18 , , , 10_1101-2021_01_07_425723 231 19 they -PRON- PRP 10_1101-2021_01_07_425723 231 20 do do VBP 10_1101-2021_01_07_425723 231 21 not not RB 10_1101-2021_01_07_425723 231 22 exclude exclude VB 10_1101-2021_01_07_425723 231 23 339 339 CD 10_1101-2021_01_07_425723 231 24 involvement involvement NN 10_1101-2021_01_07_425723 231 25 of of IN 10_1101-2021_01_07_425723 231 26 additional additional JJ 10_1101-2021_01_07_425723 231 27 oxygen oxygen NN 10_1101-2021_01_07_425723 231 28 - - HYPH 10_1101-2021_01_07_425723 231 29 requiring require VBG 10_1101-2021_01_07_425723 231 30 reactions reaction NNS 10_1101-2021_01_07_425723 231 31 in in IN 10_1101-2021_01_07_425723 231 32 other other JJ 10_1101-2021_01_07_425723 231 33 non non JJ 10_1101-2021_01_07_425723 231 34 - - JJ 10_1101-2021_01_07_425723 231 35 Saccharomyces saccharomyces JJ 10_1101-2021_01_07_425723 231 36 yeasts yeast NNS 10_1101-2021_01_07_425723 231 37 . . . 10_1101-2021_01_07_425723 232 1 For for IN 10_1101-2021_01_07_425723 232 2 example example NN 10_1101-2021_01_07_425723 232 3 , , , 10_1101-2021_01_07_425723 232 4 340 340 CD 10_1101-2021_01_07_425723 232 5 pyrimidine pyrimidine JJ 10_1101-2021_01_07_425723 232 6 biosynthesis biosynthesis NN 10_1101-2021_01_07_425723 232 7 is be VBZ 10_1101-2021_01_07_425723 232 8 often often RB 10_1101-2021_01_07_425723 232 9 cited cite VBN 10_1101-2021_01_07_425723 232 10 as as IN 10_1101-2021_01_07_425723 232 11 a a DT 10_1101-2021_01_07_425723 232 12 key key JJ 10_1101-2021_01_07_425723 232 13 oxygen oxygen NN 10_1101-2021_01_07_425723 232 14 - - HYPH 10_1101-2021_01_07_425723 232 15 requiring require VBG 10_1101-2021_01_07_425723 232 16 process process NN 10_1101-2021_01_07_425723 232 17 in in IN 10_1101-2021_01_07_425723 232 18 non non JJ 10_1101-2021_01_07_425723 232 19 - - JJ 10_1101-2021_01_07_425723 232 20 Saccharomyces saccharomyces JJ 10_1101-2021_01_07_425723 232 21 yeasts yeast NNS 10_1101-2021_01_07_425723 232 22 , , , 10_1101-2021_01_07_425723 232 23 341 341 CD 10_1101-2021_01_07_425723 232 24 due due IN 10_1101-2021_01_07_425723 232 25 to to IN 10_1101-2021_01_07_425723 232 26 involvement involvement NN 10_1101-2021_01_07_425723 232 27 of of IN 10_1101-2021_01_07_425723 232 28 a a DT 10_1101-2021_01_07_425723 232 29 respiratory respiratory JJ 10_1101-2021_01_07_425723 232 30 - - HYPH 10_1101-2021_01_07_425723 232 31 chain chain NN 10_1101-2021_01_07_425723 232 32 - - HYPH 10_1101-2021_01_07_425723 232 33 linked link VBN 10_1101-2021_01_07_425723 232 34 dihydroorotate dihydroorotate JJ 10_1101-2021_01_07_425723 232 35 dehydrogenase dehydrogenase NN 10_1101-2021_01_07_425723 232 36 ( ( -LRB- 10_1101-2021_01_07_425723 232 37 DHOD)53,54 dhod)53,54 NN 10_1101-2021_01_07_425723 232 38 . . . 10_1101-2021_01_07_425723 233 1 K. K. NNP 10_1101-2021_01_07_425723 233 2 342 342 CD 10_1101-2021_01_07_425723 233 3 marxianus marxianus NN 10_1101-2021_01_07_425723 233 4 , , , 10_1101-2021_01_07_425723 233 5 is be VBZ 10_1101-2021_01_07_425723 233 6 among among IN 10_1101-2021_01_07_425723 233 7 a a DT 10_1101-2021_01_07_425723 233 8 small small JJ 10_1101-2021_01_07_425723 233 9 number number NN 10_1101-2021_01_07_425723 233 10 of of IN 10_1101-2021_01_07_425723 233 11 yeast yeast NN 10_1101-2021_01_07_425723 233 12 species specie NNS 10_1101-2021_01_07_425723 233 13 that that WDT 10_1101-2021_01_07_425723 233 14 , , , 10_1101-2021_01_07_425723 233 15 in in IN 10_1101-2021_01_07_425723 233 16 addition addition NN 10_1101-2021_01_07_425723 233 17 to to IN 10_1101-2021_01_07_425723 233 18 this this DT 10_1101-2021_01_07_425723 233 19 respiration respiration NN 10_1101-2021_01_07_425723 233 20 dependent dependent JJ 10_1101-2021_01_07_425723 233 21 343 343 CD 10_1101-2021_01_07_425723 233 22 enzyme enzyme NNS 10_1101-2021_01_07_425723 233 23 ( ( -LRB- 10_1101-2021_01_07_425723 233 24 KmUra9 KmUra9 NNP 10_1101-2021_01_07_425723 233 25 ) ) -RRB- 10_1101-2021_01_07_425723 233 26 , , , 10_1101-2021_01_07_425723 233 27 also also RB 10_1101-2021_01_07_425723 233 28 harbors harbor VBZ 10_1101-2021_01_07_425723 233 29 a a DT 10_1101-2021_01_07_425723 233 30 fumarate fumarate RB 10_1101-2021_01_07_425723 233 31 - - HYPH 10_1101-2021_01_07_425723 233 32 dependent dependent JJ 10_1101-2021_01_07_425723 233 33 DHOD DHOD NNP 10_1101-2021_01_07_425723 233 34 ( ( -LRB- 10_1101-2021_01_07_425723 233 35 KmUra1)55 KmUra1)55 NNP 10_1101-2021_01_07_425723 233 36 . . . 10_1101-2021_01_07_425723 234 1 In in IN 10_1101-2021_01_07_425723 234 2 K. K. NNP 10_1101-2021_01_07_425723 234 3 marxianus marxianus NN 10_1101-2021_01_07_425723 234 4 the the DT 10_1101-2021_01_07_425723 234 5 activation activation NN 10_1101-2021_01_07_425723 234 6 344 344 CD 10_1101-2021_01_07_425723 234 7 of of IN 10_1101-2021_01_07_425723 234 8 this this DT 10_1101-2021_01_07_425723 234 9 oxygen oxygen NN 10_1101-2021_01_07_425723 234 10 - - HYPH 10_1101-2021_01_07_425723 234 11 independent independent JJ 10_1101-2021_01_07_425723 234 12 KmUra1 KmUra1 NNP 10_1101-2021_01_07_425723 234 13 is be VBZ 10_1101-2021_01_07_425723 234 14 a a DT 10_1101-2021_01_07_425723 234 15 crucial crucial JJ 10_1101-2021_01_07_425723 234 16 adaptation adaptation NN 10_1101-2021_01_07_425723 234 17 for for IN 10_1101-2021_01_07_425723 234 18 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 234 19 pyrimidine pyrimidine JJ 10_1101-2021_01_07_425723 234 20 biosynthesis biosynthesis NN 10_1101-2021_01_07_425723 234 21 . . . 10_1101-2021_01_07_425723 235 1 The the DT 10_1101-2021_01_07_425723 235 2 345 345 CD 10_1101-2021_01_07_425723 235 3 experimental experimental JJ 10_1101-2021_01_07_425723 235 4 approach approach NN 10_1101-2021_01_07_425723 235 5 followed follow VBN 10_1101-2021_01_07_425723 235 6 in in IN 10_1101-2021_01_07_425723 235 7 the the DT 10_1101-2021_01_07_425723 235 8 present present JJ 10_1101-2021_01_07_425723 235 9 study study NN 10_1101-2021_01_07_425723 235 10 should should MD 10_1101-2021_01_07_425723 235 11 be be VB 10_1101-2021_01_07_425723 235 12 applicable applicable JJ 10_1101-2021_01_07_425723 235 13 to to TO 10_1101-2021_01_07_425723 235 14 resolve resolve VB 10_1101-2021_01_07_425723 235 15 the the DT 10_1101-2021_01_07_425723 235 16 role role NN 10_1101-2021_01_07_425723 235 17 of of IN 10_1101-2021_01_07_425723 235 18 346 346 CD 10_1101-2021_01_07_425723 235 19 pyrimidine pyrimidine JJ 10_1101-2021_01_07_425723 235 20 biosynthesis biosynthesis NN 10_1101-2021_01_07_425723 235 21 and and CC 10_1101-2021_01_07_425723 235 22 other other JJ 10_1101-2021_01_07_425723 235 23 oxygen oxygen NN 10_1101-2021_01_07_425723 235 24 - - HYPH 10_1101-2021_01_07_425723 235 25 requiring require VBG 10_1101-2021_01_07_425723 235 26 reactions reaction NNS 10_1101-2021_01_07_425723 235 27 in in IN 10_1101-2021_01_07_425723 235 28 additional additional JJ 10_1101-2021_01_07_425723 235 29 yeast yeast NN 10_1101-2021_01_07_425723 235 30 species specie NNS 10_1101-2021_01_07_425723 235 31 . . . 10_1101-2021_01_07_425723 236 1 347 347 CD 10_1101-2021_01_07_425723 236 2 Enabling enable VBG 10_1101-2021_01_07_425723 236 3 K. K. NNP 10_1101-2021_01_07_425723 236 4 marxianus marxianus NN 10_1101-2021_01_07_425723 236 5 to to TO 10_1101-2021_01_07_425723 236 6 grow grow VB 10_1101-2021_01_07_425723 236 7 anaerobically anaerobically RB 10_1101-2021_01_07_425723 236 8 represents represent VBZ 10_1101-2021_01_07_425723 236 9 an an DT 10_1101-2021_01_07_425723 236 10 important important JJ 10_1101-2021_01_07_425723 236 11 step step NN 10_1101-2021_01_07_425723 236 12 towards towards IN 10_1101-2021_01_07_425723 236 13 application application NN 10_1101-2021_01_07_425723 236 14 of of IN 10_1101-2021_01_07_425723 236 15 this this DT 10_1101-2021_01_07_425723 236 16 348 348 CD 10_1101-2021_01_07_425723 236 17 thermotolerant thermotolerant JJ 10_1101-2021_01_07_425723 236 18 yeast yeast NN 10_1101-2021_01_07_425723 236 19 in in IN 10_1101-2021_01_07_425723 236 20 large large JJ 10_1101-2021_01_07_425723 236 21 - - HYPH 10_1101-2021_01_07_425723 236 22 scale scale NN 10_1101-2021_01_07_425723 236 23 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 236 24 bioprocesses bioprocesse NNS 10_1101-2021_01_07_425723 236 25 . . . 10_1101-2021_01_07_425723 237 1 However however RB 10_1101-2021_01_07_425723 237 2 , , , 10_1101-2021_01_07_425723 237 3 specific specific JJ 10_1101-2021_01_07_425723 237 4 growth growth NN 10_1101-2021_01_07_425723 237 5 rates rate NNS 10_1101-2021_01_07_425723 237 6 and and CC 10_1101-2021_01_07_425723 237 7 biomass biomass NN 10_1101-2021_01_07_425723 237 8 349 349 CD 10_1101-2021_01_07_425723 237 9 yields yield NNS 10_1101-2021_01_07_425723 237 10 of of IN 10_1101-2021_01_07_425723 237 11 tetrahymanol tetrahymanol NN 10_1101-2021_01_07_425723 237 12 - - HYPH 10_1101-2021_01_07_425723 237 13 expressing express VBG 10_1101-2021_01_07_425723 237 14 K. K. NNP 10_1101-2021_01_07_425723 237 15 marxianus marxianus NN 10_1101-2021_01_07_425723 237 16 in in IN 10_1101-2021_01_07_425723 237 17 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 237 18 cultures culture NNS 10_1101-2021_01_07_425723 237 19 were be VBD 10_1101-2021_01_07_425723 237 20 lower low JJR 10_1101-2021_01_07_425723 237 21 than than IN 10_1101-2021_01_07_425723 237 22 those those DT 10_1101-2021_01_07_425723 237 23 of of IN 10_1101-2021_01_07_425723 237 24 wild wild JJ 10_1101-2021_01_07_425723 237 25 - - HYPH 10_1101-2021_01_07_425723 237 26 type type NN 10_1101-2021_01_07_425723 237 27 350 350 CD 10_1101-2021_01_07_425723 237 28 S. S. NNP 10_1101-2021_01_07_425723 237 29 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 237 30 strains strain VBZ 10_1101-2021_01_07_425723 237 31 . . . 10_1101-2021_01_07_425723 238 1 A a DT 10_1101-2021_01_07_425723 238 2 similar similar JJ 10_1101-2021_01_07_425723 238 3 phenotype phenotype NN 10_1101-2021_01_07_425723 238 4 of of IN 10_1101-2021_01_07_425723 238 5 tetrahymanol tetrahymanol NN 10_1101-2021_01_07_425723 238 6 - - HYPH 10_1101-2021_01_07_425723 238 7 producing produce VBG 10_1101-2021_01_07_425723 238 8 S. S. NNP 10_1101-2021_01_07_425723 238 9 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 238 10 was be VBD 10_1101-2021_01_07_425723 238 11 proposed propose VBN 10_1101-2021_01_07_425723 238 12 to to IN 10_1101-2021_01_07_425723 238 13 351 351 CD 10_1101-2021_01_07_425723 238 14 reflect reflect VB 10_1101-2021_01_07_425723 238 15 an an DT 10_1101-2021_01_07_425723 238 16 increased increase VBN 10_1101-2021_01_07_425723 238 17 membrane membrane NN 10_1101-2021_01_07_425723 238 18 permeability46 permeability46 NNS 10_1101-2021_01_07_425723 238 19 . . . 10_1101-2021_01_07_425723 239 1 Additional additional JJ 10_1101-2021_01_07_425723 239 2 membrane membrane NN 10_1101-2021_01_07_425723 239 3 engineering engineering NN 10_1101-2021_01_07_425723 239 4 or or CC 10_1101-2021_01_07_425723 239 5 expression expression NN 10_1101-2021_01_07_425723 239 6 of of IN 10_1101-2021_01_07_425723 239 7 a a DT 10_1101-2021_01_07_425723 239 8 352 352 CD 10_1101-2021_01_07_425723 239 9 functional functional JJ 10_1101-2021_01_07_425723 239 10 sterol sterol JJ 10_1101-2021_01_07_425723 239 11 transport transport NN 10_1101-2021_01_07_425723 239 12 system system NN 10_1101-2021_01_07_425723 239 13 is be VBZ 10_1101-2021_01_07_425723 239 14 therefore therefore RB 10_1101-2021_01_07_425723 239 15 required require VBN 10_1101-2021_01_07_425723 239 16 for for IN 10_1101-2021_01_07_425723 239 17 further further JJ 10_1101-2021_01_07_425723 239 18 development development NN 10_1101-2021_01_07_425723 239 19 of of IN 10_1101-2021_01_07_425723 239 20 robust robust JJ 10_1101-2021_01_07_425723 239 21 , , , 10_1101-2021_01_07_425723 239 22 anaerobically anaerobically RB 10_1101-2021_01_07_425723 239 23 353 353 CD 10_1101-2021_01_07_425723 239 24 growing grow VBG 10_1101-2021_01_07_425723 239 25 industrial industrial JJ 10_1101-2021_01_07_425723 239 26 strains strain NNS 10_1101-2021_01_07_425723 239 27 of of IN 10_1101-2021_01_07_425723 239 28 K. K. NNP 10_1101-2021_01_07_425723 239 29 marxianus56 marxianus56 NNP 10_1101-2021_01_07_425723 239 30 . . . 10_1101-2021_01_07_425723 240 1 354 354 CD 10_1101-2021_01_07_425723 240 2 .CC .CC : 10_1101-2021_01_07_425723 240 3 - - HYPH 10_1101-2021_01_07_425723 240 4 BY by IN 10_1101-2021_01_07_425723 240 5 - - HYPH 10_1101-2021_01_07_425723 240 6 NC NC NNP 10_1101-2021_01_07_425723 240 7 - - HYPH 10_1101-2021_01_07_425723 240 8 ND ND NNP 10_1101-2021_01_07_425723 240 9 4.0 4.0 CD 10_1101-2021_01_07_425723 240 10 International International NNP 10_1101-2021_01_07_425723 240 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 240 12 under under IN 10_1101-2021_01_07_425723 240 13 a a DT 10_1101-2021_01_07_425723 240 14 ( ( -LRB- 10_1101-2021_01_07_425723 240 15 which which WDT 10_1101-2021_01_07_425723 240 16 was be VBD 10_1101-2021_01_07_425723 240 17 not not RB 10_1101-2021_01_07_425723 240 18 certified certify VBN 10_1101-2021_01_07_425723 240 19 by by IN 10_1101-2021_01_07_425723 240 20 peer peer NN 10_1101-2021_01_07_425723 240 21 review review NN 10_1101-2021_01_07_425723 240 22 ) ) -RRB- 10_1101-2021_01_07_425723 240 23 is be VBZ 10_1101-2021_01_07_425723 240 24 the the DT 10_1101-2021_01_07_425723 240 25 author author NN 10_1101-2021_01_07_425723 240 26 / / SYM 10_1101-2021_01_07_425723 240 27 funder funder NN 10_1101-2021_01_07_425723 240 28 , , , 10_1101-2021_01_07_425723 240 29 who who WP 10_1101-2021_01_07_425723 240 30 has have VBZ 10_1101-2021_01_07_425723 240 31 granted grant VBN 10_1101-2021_01_07_425723 240 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 240 33 a a DT 10_1101-2021_01_07_425723 240 34 license license NN 10_1101-2021_01_07_425723 240 35 to to TO 10_1101-2021_01_07_425723 240 36 display display VB 10_1101-2021_01_07_425723 240 37 the the DT 10_1101-2021_01_07_425723 240 38 preprint preprint NN 10_1101-2021_01_07_425723 240 39 in in IN 10_1101-2021_01_07_425723 240 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 240 41 . . . 10_1101-2021_01_07_425723 241 1 It -PRON- PRP 10_1101-2021_01_07_425723 241 2 is be VBZ 10_1101-2021_01_07_425723 241 3 made make VBN 10_1101-2021_01_07_425723 241 4 The the DT 10_1101-2021_01_07_425723 241 5 copyright copyright NN 10_1101-2021_01_07_425723 241 6 holder holder NN 10_1101-2021_01_07_425723 241 7 for for IN 10_1101-2021_01_07_425723 241 8 this this DT 10_1101-2021_01_07_425723 241 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 241 10 version version NN 10_1101-2021_01_07_425723 241 11 posted post VBD 10_1101-2021_01_07_425723 241 12 January January NNP 10_1101-2021_01_07_425723 241 13 8 8 CD 10_1101-2021_01_07_425723 241 14 , , , 10_1101-2021_01_07_425723 241 15 2021 2021 CD 10_1101-2021_01_07_425723 241 16 . . . 10_1101-2021_01_07_425723 241 17 ; ; : 10_1101-2021_01_07_425723 241 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 241 19 : : : 10_1101-2021_01_07_425723 241 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 241 21 preprint preprint NN 10_1101-2021_01_07_425723 241 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 241 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 241 24 22 22 CD 10_1101-2021_01_07_425723 241 25 online online JJ 10_1101-2021_01_07_425723 241 26 Methods Methods NNP 10_1101-2021_01_07_425723 241 27 355 355 CD 10_1101-2021_01_07_425723 241 28 Yeast yeast NN 10_1101-2021_01_07_425723 241 29 strains strain NNS 10_1101-2021_01_07_425723 241 30 , , , 10_1101-2021_01_07_425723 241 31 maintenance maintenance NN 10_1101-2021_01_07_425723 241 32 and and CC 10_1101-2021_01_07_425723 241 33 shake shake NN 10_1101-2021_01_07_425723 241 34 - - HYPH 10_1101-2021_01_07_425723 241 35 flask flask NN 10_1101-2021_01_07_425723 241 36 cultivation cultivation NN 10_1101-2021_01_07_425723 241 37 356 356 CD 10_1101-2021_01_07_425723 241 38 Saccharomyces Saccharomyces NNPS 10_1101-2021_01_07_425723 241 39 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 241 40 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 241 41 - - HYPH 10_1101-2021_01_07_425723 241 42 7D57,58 7d57,58 CD 10_1101-2021_01_07_425723 241 43 ( ( -LRB- 10_1101-2021_01_07_425723 241 44 MATa MATa NNPS 10_1101-2021_01_07_425723 241 45 MAL2 MAL2 NNP 10_1101-2021_01_07_425723 241 46 - - HYPH 10_1101-2021_01_07_425723 241 47 8c 8c NNP 10_1101-2021_01_07_425723 241 48 SUC2 SUC2 NNP 10_1101-2021_01_07_425723 241 49 ) ) -RRB- 10_1101-2021_01_07_425723 241 50 was be VBD 10_1101-2021_01_07_425723 241 51 obtained obtain VBN 10_1101-2021_01_07_425723 241 52 from from IN 10_1101-2021_01_07_425723 241 53 Dr. Dr. NNP 10_1101-2021_01_07_425723 241 54 Peter Peter NNP 10_1101-2021_01_07_425723 241 55 Kötter Kötter NNP 10_1101-2021_01_07_425723 241 56 , , , 10_1101-2021_01_07_425723 241 57 357 357 CD 10_1101-2021_01_07_425723 241 58 J.W. J.W. NNP 10_1101-2021_01_07_425723 242 1 Goethe Goethe NNP 10_1101-2021_01_07_425723 242 2 University University NNP 10_1101-2021_01_07_425723 242 3 , , , 10_1101-2021_01_07_425723 242 4 Frankfurt Frankfurt NNP 10_1101-2021_01_07_425723 242 5 . . . 10_1101-2021_01_07_425723 243 1 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 243 2 marxianus marxianus NN 10_1101-2021_01_07_425723 243 3 strains strain VBZ 10_1101-2021_01_07_425723 243 4 CBS CBS NNP 10_1101-2021_01_07_425723 243 5 6556 6556 CD 10_1101-2021_01_07_425723 243 6 ( ( -LRB- 10_1101-2021_01_07_425723 243 7 ATCC ATCC NNP 10_1101-2021_01_07_425723 243 8 26548 26548 CD 10_1101-2021_01_07_425723 243 9 ; ; : 10_1101-2021_01_07_425723 243 10 NCYC NCYC NNP 10_1101-2021_01_07_425723 243 11 2597 2597 CD 10_1101-2021_01_07_425723 243 12 ; ; : 10_1101-2021_01_07_425723 243 13 358 358 CD 10_1101-2021_01_07_425723 243 14 NRRL NRRL NNP 10_1101-2021_01_07_425723 243 15 Y-7571 y-7571 NN 10_1101-2021_01_07_425723 243 16 ) ) -RRB- 10_1101-2021_01_07_425723 243 17 and and CC 10_1101-2021_01_07_425723 243 18 NBRC NBRC NNP 10_1101-2021_01_07_425723 243 19 1777 1777 CD 10_1101-2021_01_07_425723 243 20 ( ( -LRB- 10_1101-2021_01_07_425723 243 21 IFO IFO NNP 10_1101-2021_01_07_425723 243 22 1777 1777 CD 10_1101-2021_01_07_425723 243 23 ) ) -RRB- 10_1101-2021_01_07_425723 243 24 were be VBD 10_1101-2021_01_07_425723 243 25 obtained obtain VBN 10_1101-2021_01_07_425723 243 26 from from IN 10_1101-2021_01_07_425723 243 27 the the DT 10_1101-2021_01_07_425723 243 28 Westerdijk Westerdijk NNP 10_1101-2021_01_07_425723 243 29 Fungal Fungal NNP 10_1101-2021_01_07_425723 243 30 Biodiversity Biodiversity NNP 10_1101-2021_01_07_425723 243 31 359 359 CD 10_1101-2021_01_07_425723 243 32 Institute Institute NNP 10_1101-2021_01_07_425723 243 33 ( ( -LRB- 10_1101-2021_01_07_425723 243 34 Utrecht Utrecht NNP 10_1101-2021_01_07_425723 243 35 , , , 10_1101-2021_01_07_425723 243 36 The the DT 10_1101-2021_01_07_425723 243 37 Netherlands Netherlands NNP 10_1101-2021_01_07_425723 243 38 ) ) -RRB- 10_1101-2021_01_07_425723 243 39 and and CC 10_1101-2021_01_07_425723 243 40 the the DT 10_1101-2021_01_07_425723 243 41 Biological Biological NNP 10_1101-2021_01_07_425723 243 42 Resource Resource NNP 10_1101-2021_01_07_425723 243 43 Center Center NNP 10_1101-2021_01_07_425723 243 44 , , , 10_1101-2021_01_07_425723 243 45 NITE NITE NNP 10_1101-2021_01_07_425723 243 46 ( ( -LRB- 10_1101-2021_01_07_425723 243 47 NBRC NBRC NNP 10_1101-2021_01_07_425723 243 48 ) ) -RRB- 10_1101-2021_01_07_425723 243 49 ( ( -LRB- 10_1101-2021_01_07_425723 243 50 Chiba Chiba NNP 10_1101-2021_01_07_425723 243 51 , , , 10_1101-2021_01_07_425723 243 52 Japan Japan NNP 10_1101-2021_01_07_425723 243 53 ) ) -RRB- 10_1101-2021_01_07_425723 243 54 , , , 10_1101-2021_01_07_425723 243 55 360 360 CD 10_1101-2021_01_07_425723 243 56 respectively respectively RB 10_1101-2021_01_07_425723 243 57 . . . 10_1101-2021_01_07_425723 244 1 Stock stock NN 10_1101-2021_01_07_425723 244 2 cultures culture NNS 10_1101-2021_01_07_425723 244 3 of of IN 10_1101-2021_01_07_425723 244 4 S. S. NNP 10_1101-2021_01_07_425723 244 5 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 244 6 were be VBD 10_1101-2021_01_07_425723 244 7 grown grow VBN 10_1101-2021_01_07_425723 244 8 at at IN 10_1101-2021_01_07_425723 244 9 30 30 CD 10_1101-2021_01_07_425723 244 10 ° ° , 10_1101-2021_01_07_425723 244 11 C C NNP 10_1101-2021_01_07_425723 244 12 in in IN 10_1101-2021_01_07_425723 244 13 an an DT 10_1101-2021_01_07_425723 244 14 orbital orbital JJ 10_1101-2021_01_07_425723 244 15 shaker shaker NN 10_1101-2021_01_07_425723 244 16 set set VBN 10_1101-2021_01_07_425723 244 17 at at IN 10_1101-2021_01_07_425723 244 18 200 200 CD 10_1101-2021_01_07_425723 244 19 rpm rpm NN 10_1101-2021_01_07_425723 244 20 , , , 10_1101-2021_01_07_425723 244 21 in in IN 10_1101-2021_01_07_425723 244 22 361 361 CD 10_1101-2021_01_07_425723 244 23 500 500 CD 10_1101-2021_01_07_425723 244 24 mL mL NNP 10_1101-2021_01_07_425723 244 25 shake shake VBP 10_1101-2021_01_07_425723 244 26 flasks flask NNS 10_1101-2021_01_07_425723 244 27 containing contain VBG 10_1101-2021_01_07_425723 244 28 100 100 CD 10_1101-2021_01_07_425723 244 29 mL mL NNP 10_1101-2021_01_07_425723 244 30 YPD YPD NNP 10_1101-2021_01_07_425723 244 31 ( ( -LRB- 10_1101-2021_01_07_425723 244 32 10 10 CD 10_1101-2021_01_07_425723 244 33 g·L-1 g·l-1 CD 10_1101-2021_01_07_425723 244 34 Bacto Bacto NNP 10_1101-2021_01_07_425723 244 35 yeast yeast NN 10_1101-2021_01_07_425723 244 36 extract extract NN 10_1101-2021_01_07_425723 244 37 , , , 10_1101-2021_01_07_425723 244 38 20 20 CD 10_1101-2021_01_07_425723 244 39 g·L-1 g·l-1 CD 10_1101-2021_01_07_425723 244 40 Bacto Bacto NNP 10_1101-2021_01_07_425723 244 41 peptone peptone NN 10_1101-2021_01_07_425723 244 42 , , , 10_1101-2021_01_07_425723 244 43 20 20 CD 10_1101-2021_01_07_425723 244 44 g·L-1 g·L-1 NNP 10_1101-2021_01_07_425723 244 45 362 362 CD 10_1101-2021_01_07_425723 244 46 glucose glucose NN 10_1101-2021_01_07_425723 244 47 ) ) -RRB- 10_1101-2021_01_07_425723 244 48 . . . 10_1101-2021_01_07_425723 245 1 For for IN 10_1101-2021_01_07_425723 245 2 cultures culture NNS 10_1101-2021_01_07_425723 245 3 of of IN 10_1101-2021_01_07_425723 245 4 K. K. NNP 10_1101-2021_01_07_425723 245 5 marxianus marxianus NNP 10_1101-2021_01_07_425723 245 6 , , , 10_1101-2021_01_07_425723 245 7 the the DT 10_1101-2021_01_07_425723 245 8 glucose glucose NNP 10_1101-2021_01_07_425723 245 9 concentration concentration NN 10_1101-2021_01_07_425723 245 10 was be VBD 10_1101-2021_01_07_425723 245 11 reduced reduce VBN 10_1101-2021_01_07_425723 245 12 to to IN 10_1101-2021_01_07_425723 245 13 7.5 7.5 CD 10_1101-2021_01_07_425723 245 14 g·L-1 g·L-1 NNP 10_1101-2021_01_07_425723 245 15 . . . 10_1101-2021_01_07_425723 246 1 After after IN 10_1101-2021_01_07_425723 246 2 addition addition NN 10_1101-2021_01_07_425723 246 3 363 363 CD 10_1101-2021_01_07_425723 246 4 of of IN 10_1101-2021_01_07_425723 246 5 glycerol glycerol NN 10_1101-2021_01_07_425723 246 6 to to IN 10_1101-2021_01_07_425723 246 7 early early JJ 10_1101-2021_01_07_425723 246 8 stationary stationary JJ 10_1101-2021_01_07_425723 246 9 - - HYPH 10_1101-2021_01_07_425723 246 10 phase phase NN 10_1101-2021_01_07_425723 246 11 cultures culture NNS 10_1101-2021_01_07_425723 246 12 , , , 10_1101-2021_01_07_425723 246 13 to to IN 10_1101-2021_01_07_425723 246 14 a a DT 10_1101-2021_01_07_425723 246 15 concentration concentration NN 10_1101-2021_01_07_425723 246 16 of of IN 10_1101-2021_01_07_425723 246 17 30 30 CD 10_1101-2021_01_07_425723 246 18 % % NN 10_1101-2021_01_07_425723 246 19 ( ( -LRB- 10_1101-2021_01_07_425723 246 20 v v NN 10_1101-2021_01_07_425723 246 21 / / SYM 10_1101-2021_01_07_425723 246 22 v v NNP 10_1101-2021_01_07_425723 246 23 ) ) -RRB- 10_1101-2021_01_07_425723 246 24 , , , 10_1101-2021_01_07_425723 246 25 2 2 CD 10_1101-2021_01_07_425723 246 26 mL mL NNP 10_1101-2021_01_07_425723 246 27 aliquots aliquot NNS 10_1101-2021_01_07_425723 246 28 were be VBD 10_1101-2021_01_07_425723 246 29 stored store VBN 10_1101-2021_01_07_425723 246 30 364 364 CD 10_1101-2021_01_07_425723 246 31 at at IN 10_1101-2021_01_07_425723 246 32 -80 -80 NNP 10_1101-2021_01_07_425723 246 33 ° ° NNP 10_1101-2021_01_07_425723 246 34 C C NNP 10_1101-2021_01_07_425723 246 35 . . . 10_1101-2021_01_07_425723 247 1 Shake shake NN 10_1101-2021_01_07_425723 247 2 - - HYPH 10_1101-2021_01_07_425723 247 3 flask flask NN 10_1101-2021_01_07_425723 247 4 precultures preculture NNS 10_1101-2021_01_07_425723 247 5 for for IN 10_1101-2021_01_07_425723 247 6 bioreactor bioreactor NN 10_1101-2021_01_07_425723 247 7 experiments experiment NNS 10_1101-2021_01_07_425723 247 8 were be VBD 10_1101-2021_01_07_425723 247 9 grown grow VBN 10_1101-2021_01_07_425723 247 10 in in IN 10_1101-2021_01_07_425723 247 11 100 100 CD 10_1101-2021_01_07_425723 247 12 mL mL NNP 10_1101-2021_01_07_425723 247 13 synthetic synthetic JJ 10_1101-2021_01_07_425723 247 14 medium medium NN 10_1101-2021_01_07_425723 247 15 365 365 CD 10_1101-2021_01_07_425723 247 16 ( ( -LRB- 10_1101-2021_01_07_425723 247 17 SM SM NNP 10_1101-2021_01_07_425723 247 18 ) ) -RRB- 10_1101-2021_01_07_425723 247 19 with with IN 10_1101-2021_01_07_425723 247 20 glucose glucose NN 10_1101-2021_01_07_425723 247 21 as as IN 10_1101-2021_01_07_425723 247 22 carbon carbon NN 10_1101-2021_01_07_425723 247 23 source source NN 10_1101-2021_01_07_425723 247 24 and and CC 10_1101-2021_01_07_425723 247 25 urea urea NNP 10_1101-2021_01_07_425723 247 26 as as IN 10_1101-2021_01_07_425723 247 27 nitrogen nitrogen NN 10_1101-2021_01_07_425723 247 28 source source NN 10_1101-2021_01_07_425723 247 29 ( ( -LRB- 10_1101-2021_01_07_425723 247 30 SMG smg NN 10_1101-2021_01_07_425723 247 31 - - HYPH 10_1101-2021_01_07_425723 247 32 urea)17,59 urea)17,59 NNP 10_1101-2021_01_07_425723 247 33 . . . 10_1101-2021_01_07_425723 248 1 For for IN 10_1101-2021_01_07_425723 248 2 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 248 3 366 366 CD 10_1101-2021_01_07_425723 248 4 cultivation cultivation NN 10_1101-2021_01_07_425723 248 5 , , , 10_1101-2021_01_07_425723 248 6 synthetic synthetic JJ 10_1101-2021_01_07_425723 248 7 medium medium NN 10_1101-2021_01_07_425723 248 8 was be VBD 10_1101-2021_01_07_425723 248 9 supplemented supplement VBN 10_1101-2021_01_07_425723 248 10 with with IN 10_1101-2021_01_07_425723 248 11 ergosterol ergosterol NN 10_1101-2021_01_07_425723 248 12 ( ( -LRB- 10_1101-2021_01_07_425723 248 13 10 10 CD 10_1101-2021_01_07_425723 248 14 mg·L-1 mg·l-1 NN 10_1101-2021_01_07_425723 248 15 ) ) -RRB- 10_1101-2021_01_07_425723 248 16 and and CC 10_1101-2021_01_07_425723 248 17 Tween Tween NNP 10_1101-2021_01_07_425723 248 18 80 80 CD 10_1101-2021_01_07_425723 248 19 ( ( -LRB- 10_1101-2021_01_07_425723 248 20 420 420 CD 10_1101-2021_01_07_425723 248 21 mg·L-1 mg·l-1 NN 10_1101-2021_01_07_425723 248 22 ) ) -RRB- 10_1101-2021_01_07_425723 248 23 367 367 CD 10_1101-2021_01_07_425723 248 24 as as IN 10_1101-2021_01_07_425723 248 25 described describe VBN 10_1101-2021_01_07_425723 248 26 previously14,17,19 previously14,17,19 NN 10_1101-2021_01_07_425723 248 27 . . . 10_1101-2021_01_07_425723 249 1 368 368 CD 10_1101-2021_01_07_425723 249 2 Expression expression NN 10_1101-2021_01_07_425723 249 3 cassette cassette NN 10_1101-2021_01_07_425723 249 4 and and CC 10_1101-2021_01_07_425723 249 5 plasmid plasmid NN 10_1101-2021_01_07_425723 249 6 construction construction NN 10_1101-2021_01_07_425723 249 7 369 369 CD 10_1101-2021_01_07_425723 249 8 Plasmids Plasmids NNPS 10_1101-2021_01_07_425723 249 9 used use VBN 10_1101-2021_01_07_425723 249 10 in in IN 10_1101-2021_01_07_425723 249 11 this this DT 10_1101-2021_01_07_425723 249 12 study study NN 10_1101-2021_01_07_425723 249 13 are be VBP 10_1101-2021_01_07_425723 249 14 described describe VBN 10_1101-2021_01_07_425723 249 15 in in IN 10_1101-2021_01_07_425723 249 16 ( ( -LRB- 10_1101-2021_01_07_425723 249 17 Table table NN 10_1101-2021_01_07_425723 249 18 4 4 CD 10_1101-2021_01_07_425723 249 19 ) ) -RRB- 10_1101-2021_01_07_425723 249 20 . . . 10_1101-2021_01_07_425723 250 1 To to TO 10_1101-2021_01_07_425723 250 2 construct construct VB 10_1101-2021_01_07_425723 250 3 plasmids plasmids NNP 10_1101-2021_01_07_425723 250 4 pUDE659 pUDE659 NNP 10_1101-2021_01_07_425723 250 5 ( ( -LRB- 10_1101-2021_01_07_425723 250 6 gRNAAUS1 grnaaus1 NN 10_1101-2021_01_07_425723 250 7 ) ) -RRB- 10_1101-2021_01_07_425723 250 8 and and CC 10_1101-2021_01_07_425723 250 9 370 370 CD 10_1101-2021_01_07_425723 250 10 pUDE663 pUDE663 NNS 10_1101-2021_01_07_425723 250 11 ( ( -LRB- 10_1101-2021_01_07_425723 250 12 gRNAPDR11 gRNAPDR11 NNP 10_1101-2021_01_07_425723 250 13 ) ) -RRB- 10_1101-2021_01_07_425723 250 14 , , , 10_1101-2021_01_07_425723 250 15 the the DT 10_1101-2021_01_07_425723 250 16 pROS11 pROS11 NNP 10_1101-2021_01_07_425723 250 17 plasmid plasmid NN 10_1101-2021_01_07_425723 250 18 - - HYPH 10_1101-2021_01_07_425723 250 19 backbone backbone NN 10_1101-2021_01_07_425723 250 20 was be VBD 10_1101-2021_01_07_425723 250 21 PCR PCR NNP 10_1101-2021_01_07_425723 250 22 amplified amplify VBD 10_1101-2021_01_07_425723 250 23 using use VBG 10_1101-2021_01_07_425723 250 24 Phusion Phusion NNP 10_1101-2021_01_07_425723 250 25 HF HF NNP 10_1101-2021_01_07_425723 250 26 polymerase polymerase NN 10_1101-2021_01_07_425723 250 27 371 371 CD 10_1101-2021_01_07_425723 250 28 ( ( -LRB- 10_1101-2021_01_07_425723 250 29 Thermo Thermo NNP 10_1101-2021_01_07_425723 250 30 Scientific Scientific NNP 10_1101-2021_01_07_425723 250 31 , , , 10_1101-2021_01_07_425723 250 32 Waltham Waltham NNP 10_1101-2021_01_07_425723 250 33 , , , 10_1101-2021_01_07_425723 250 34 MA MA NNP 10_1101-2021_01_07_425723 250 35 ) ) -RRB- 10_1101-2021_01_07_425723 250 36 with with IN 10_1101-2021_01_07_425723 250 37 the the DT 10_1101-2021_01_07_425723 250 38 double double RB 10_1101-2021_01_07_425723 250 39 - - HYPH 10_1101-2021_01_07_425723 250 40 binding bind VBG 10_1101-2021_01_07_425723 250 41 primer primer NN 10_1101-2021_01_07_425723 250 42 6005 6005 CD 10_1101-2021_01_07_425723 250 43 . . . 10_1101-2021_01_07_425723 251 1 PCR PCR NNP 10_1101-2021_01_07_425723 251 2 amplifications amplification NNS 10_1101-2021_01_07_425723 251 3 were be VBD 10_1101-2021_01_07_425723 251 4 372 372 CD 10_1101-2021_01_07_425723 251 5 performed perform VBN 10_1101-2021_01_07_425723 251 6 with with IN 10_1101-2021_01_07_425723 251 7 desalted desalt VBN 10_1101-2021_01_07_425723 251 8 or or CC 10_1101-2021_01_07_425723 251 9 PAGE page NN 10_1101-2021_01_07_425723 251 10 - - HYPH 10_1101-2021_01_07_425723 251 11 purified purify VBN 10_1101-2021_01_07_425723 251 12 oligonucleotide oligonucleotide JJ 10_1101-2021_01_07_425723 251 13 primers primer NNS 10_1101-2021_01_07_425723 251 14 ( ( -LRB- 10_1101-2021_01_07_425723 251 15 Sigma Sigma NNP 10_1101-2021_01_07_425723 251 16 - - HYPH 10_1101-2021_01_07_425723 251 17 Aldrich Aldrich NNP 10_1101-2021_01_07_425723 251 18 , , , 10_1101-2021_01_07_425723 251 19 St St NNP 10_1101-2021_01_07_425723 251 20 Louis Louis NNP 10_1101-2021_01_07_425723 251 21 , , , 10_1101-2021_01_07_425723 251 22 MO MO NNP 10_1101-2021_01_07_425723 251 23 ) ) -RRB- 10_1101-2021_01_07_425723 251 24 373 373 CD 10_1101-2021_01_07_425723 251 25 according accord VBG 10_1101-2021_01_07_425723 251 26 to to IN 10_1101-2021_01_07_425723 251 27 manufacturer manufacturer NN 10_1101-2021_01_07_425723 251 28 ’s ’s POS 10_1101-2021_01_07_425723 251 29 instructions instruction NNS 10_1101-2021_01_07_425723 251 30 . . . 10_1101-2021_01_07_425723 252 1 To to TO 10_1101-2021_01_07_425723 252 2 introduce introduce VB 10_1101-2021_01_07_425723 252 3 the the DT 10_1101-2021_01_07_425723 252 4 gRNA grna NN 10_1101-2021_01_07_425723 252 5 - - HYPH 10_1101-2021_01_07_425723 252 6 encoding encode VBG 10_1101-2021_01_07_425723 252 7 nucleotide nucleotide JJ 10_1101-2021_01_07_425723 252 8 sequences sequence NNS 10_1101-2021_01_07_425723 252 9 into into IN 10_1101-2021_01_07_425723 252 10 374 374 CD 10_1101-2021_01_07_425723 252 11 gRNA grna CD 10_1101-2021_01_07_425723 252 12 - - HYPH 10_1101-2021_01_07_425723 252 13 expression expression NN 10_1101-2021_01_07_425723 252 14 plasmids plasmid NNS 10_1101-2021_01_07_425723 252 15 , , , 10_1101-2021_01_07_425723 252 16 a a DT 10_1101-2021_01_07_425723 252 17 2μm 2μm JJ 10_1101-2021_01_07_425723 252 18 fragment fragment NN 10_1101-2021_01_07_425723 252 19 was be VBD 10_1101-2021_01_07_425723 252 20 first first RB 10_1101-2021_01_07_425723 252 21 amplified amplify VBN 10_1101-2021_01_07_425723 252 22 with with IN 10_1101-2021_01_07_425723 252 23 primers primer NNS 10_1101-2021_01_07_425723 252 24 11228 11228 CD 10_1101-2021_01_07_425723 252 25 and and CC 10_1101-2021_01_07_425723 252 26 11232 11232 CD 10_1101-2021_01_07_425723 252 27 375 375 CD 10_1101-2021_01_07_425723 252 28 containing contain VBG 10_1101-2021_01_07_425723 252 29 the the DT 10_1101-2021_01_07_425723 252 30 specific specific JJ 10_1101-2021_01_07_425723 252 31 sequence sequence NN 10_1101-2021_01_07_425723 252 32 as as IN 10_1101-2021_01_07_425723 252 33 primer primer NNP 10_1101-2021_01_07_425723 252 34 overhang overhang NN 10_1101-2021_01_07_425723 252 35 using use VBG 10_1101-2021_01_07_425723 252 36 pROS11 pROS11 NNP 10_1101-2021_01_07_425723 252 37 as as IN 10_1101-2021_01_07_425723 252 38 template template NN 10_1101-2021_01_07_425723 252 39 . . . 10_1101-2021_01_07_425723 253 1 PCR PCR NNP 10_1101-2021_01_07_425723 253 2 products product NNS 10_1101-2021_01_07_425723 253 3 were be VBD 10_1101-2021_01_07_425723 253 4 376 376 CD 10_1101-2021_01_07_425723 253 5 purified purify VBN 10_1101-2021_01_07_425723 253 6 with with IN 10_1101-2021_01_07_425723 253 7 genElutePCR genElutePCR NNP 10_1101-2021_01_07_425723 253 8 Clean Clean NNP 10_1101-2021_01_07_425723 253 9 - - HYPH 10_1101-2021_01_07_425723 253 10 Up up RP 10_1101-2021_01_07_425723 253 11 Kit kit NN 10_1101-2021_01_07_425723 253 12 ( ( -LRB- 10_1101-2021_01_07_425723 253 13 Sigma Sigma NNP 10_1101-2021_01_07_425723 253 14 - - HYPH 10_1101-2021_01_07_425723 253 15 Aldrich Aldrich NNP 10_1101-2021_01_07_425723 253 16 ) ) -RRB- 10_1101-2021_01_07_425723 253 17 or or CC 10_1101-2021_01_07_425723 253 18 Gel Gel NNP 10_1101-2021_01_07_425723 253 19 DNA DNA NNP 10_1101-2021_01_07_425723 253 20 Recovery Recovery NNP 10_1101-2021_01_07_425723 253 21 Kit Kit NNP 10_1101-2021_01_07_425723 253 22 ( ( -LRB- 10_1101-2021_01_07_425723 253 23 Zymo Zymo NNP 10_1101-2021_01_07_425723 253 24 Research Research NNP 10_1101-2021_01_07_425723 253 25 , , , 10_1101-2021_01_07_425723 253 26 Irvine Irvine NNP 10_1101-2021_01_07_425723 253 27 , , , 10_1101-2021_01_07_425723 253 28 377 377 CD 10_1101-2021_01_07_425723 253 29 .CC .CC NFP 10_1101-2021_01_07_425723 253 30 - - HYPH 10_1101-2021_01_07_425723 253 31 BY by IN 10_1101-2021_01_07_425723 253 32 - - HYPH 10_1101-2021_01_07_425723 253 33 NC NC NNP 10_1101-2021_01_07_425723 253 34 - - HYPH 10_1101-2021_01_07_425723 253 35 ND ND NNP 10_1101-2021_01_07_425723 253 36 4.0 4.0 CD 10_1101-2021_01_07_425723 253 37 International International NNP 10_1101-2021_01_07_425723 253 38 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 253 39 under under IN 10_1101-2021_01_07_425723 253 40 a a DT 10_1101-2021_01_07_425723 253 41 ( ( -LRB- 10_1101-2021_01_07_425723 253 42 which which WDT 10_1101-2021_01_07_425723 253 43 was be VBD 10_1101-2021_01_07_425723 253 44 not not RB 10_1101-2021_01_07_425723 253 45 certified certify VBN 10_1101-2021_01_07_425723 253 46 by by IN 10_1101-2021_01_07_425723 253 47 peer peer NN 10_1101-2021_01_07_425723 253 48 review review NN 10_1101-2021_01_07_425723 253 49 ) ) -RRB- 10_1101-2021_01_07_425723 253 50 is be VBZ 10_1101-2021_01_07_425723 253 51 the the DT 10_1101-2021_01_07_425723 253 52 author author NN 10_1101-2021_01_07_425723 253 53 / / SYM 10_1101-2021_01_07_425723 253 54 funder funder NN 10_1101-2021_01_07_425723 253 55 , , , 10_1101-2021_01_07_425723 253 56 who who WP 10_1101-2021_01_07_425723 253 57 has have VBZ 10_1101-2021_01_07_425723 253 58 granted grant VBN 10_1101-2021_01_07_425723 253 59 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 253 60 a a DT 10_1101-2021_01_07_425723 253 61 license license NN 10_1101-2021_01_07_425723 253 62 to to TO 10_1101-2021_01_07_425723 253 63 display display VB 10_1101-2021_01_07_425723 253 64 the the DT 10_1101-2021_01_07_425723 253 65 preprint preprint NN 10_1101-2021_01_07_425723 253 66 in in IN 10_1101-2021_01_07_425723 253 67 perpetuity perpetuity NN 10_1101-2021_01_07_425723 253 68 . . . 10_1101-2021_01_07_425723 254 1 It -PRON- PRP 10_1101-2021_01_07_425723 254 2 is be VBZ 10_1101-2021_01_07_425723 254 3 made make VBN 10_1101-2021_01_07_425723 254 4 The the DT 10_1101-2021_01_07_425723 254 5 copyright copyright NN 10_1101-2021_01_07_425723 254 6 holder holder NN 10_1101-2021_01_07_425723 254 7 for for IN 10_1101-2021_01_07_425723 254 8 this this DT 10_1101-2021_01_07_425723 254 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 254 10 version version NN 10_1101-2021_01_07_425723 254 11 posted post VBD 10_1101-2021_01_07_425723 254 12 January January NNP 10_1101-2021_01_07_425723 254 13 8 8 CD 10_1101-2021_01_07_425723 254 14 , , , 10_1101-2021_01_07_425723 254 15 2021 2021 CD 10_1101-2021_01_07_425723 254 16 . . . 10_1101-2021_01_07_425723 254 17 ; ; : 10_1101-2021_01_07_425723 254 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 254 19 : : : 10_1101-2021_01_07_425723 254 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 254 21 preprint preprint NN 10_1101-2021_01_07_425723 254 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 254 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 254 24 23 23 CD 10_1101-2021_01_07_425723 254 25 CA CA NNP 10_1101-2021_01_07_425723 254 26 ) ) -RRB- 10_1101-2021_01_07_425723 254 27 . . . 10_1101-2021_01_07_425723 255 1 The the DT 10_1101-2021_01_07_425723 255 2 two two CD 10_1101-2021_01_07_425723 255 3 DNA DNA NNP 10_1101-2021_01_07_425723 255 4 fragments fragment NNS 10_1101-2021_01_07_425723 255 5 were be VBD 10_1101-2021_01_07_425723 255 6 then then RB 10_1101-2021_01_07_425723 255 7 assembled assemble VBN 10_1101-2021_01_07_425723 255 8 by by IN 10_1101-2021_01_07_425723 255 9 Gibson Gibson NNP 10_1101-2021_01_07_425723 255 10 Assembly Assembly NNP 10_1101-2021_01_07_425723 255 11 ( ( -LRB- 10_1101-2021_01_07_425723 255 12 New New NNP 10_1101-2021_01_07_425723 255 13 England England NNP 10_1101-2021_01_07_425723 255 14 Biolabs Biolabs NNP 10_1101-2021_01_07_425723 255 15 , , , 10_1101-2021_01_07_425723 255 16 Ipswich Ipswich NNP 10_1101-2021_01_07_425723 255 17 , , , 10_1101-2021_01_07_425723 255 18 378 378 CD 10_1101-2021_01_07_425723 255 19 MA MA NNP 10_1101-2021_01_07_425723 255 20 ) ) -RRB- 10_1101-2021_01_07_425723 255 21 according accord VBG 10_1101-2021_01_07_425723 255 22 to to IN 10_1101-2021_01_07_425723 255 23 the the DT 10_1101-2021_01_07_425723 255 24 manufacturer manufacturer NN 10_1101-2021_01_07_425723 255 25 ’s ’s POS 10_1101-2021_01_07_425723 255 26 instructions instruction NNS 10_1101-2021_01_07_425723 255 27 . . . 10_1101-2021_01_07_425723 256 1 Gibson Gibson NNP 10_1101-2021_01_07_425723 256 2 assembly assembly NN 10_1101-2021_01_07_425723 256 3 reaction reaction NN 10_1101-2021_01_07_425723 256 4 volumes volume NNS 10_1101-2021_01_07_425723 256 5 were be VBD 10_1101-2021_01_07_425723 256 6 downscaled downscale VBN 10_1101-2021_01_07_425723 256 7 379 379 CD 10_1101-2021_01_07_425723 256 8 to to TO 10_1101-2021_01_07_425723 256 9 10 10 CD 10_1101-2021_01_07_425723 256 10 µL µl CD 10_1101-2021_01_07_425723 256 11 and and CC 10_1101-2021_01_07_425723 256 12 0.01 0.01 CD 10_1101-2021_01_07_425723 256 13 pmol·µL-1 pmol·µL-1 NNS 10_1101-2021_01_07_425723 256 14 DNA DNA NNP 10_1101-2021_01_07_425723 256 15 fragments fragment NNS 10_1101-2021_01_07_425723 256 16 at at IN 10_1101-2021_01_07_425723 256 17 1:1 1:1 CD 10_1101-2021_01_07_425723 256 18 molar molar JJ 10_1101-2021_01_07_425723 256 19 ratio ratio NN 10_1101-2021_01_07_425723 256 20 for for IN 10_1101-2021_01_07_425723 256 21 1 1 CD 10_1101-2021_01_07_425723 256 22 h h NN 10_1101-2021_01_07_425723 256 23 at at IN 10_1101-2021_01_07_425723 256 24 50 50 CD 10_1101-2021_01_07_425723 256 25 ° ° NNS 10_1101-2021_01_07_425723 256 26 C C NNP 10_1101-2021_01_07_425723 256 27 . . . 10_1101-2021_01_07_425723 257 1 Chemically chemically RB 10_1101-2021_01_07_425723 257 2 competent competent JJ 10_1101-2021_01_07_425723 257 3 E. e. NN 10_1101-2021_01_07_425723 257 4 380 380 CD 10_1101-2021_01_07_425723 257 5 coli coli NNS 10_1101-2021_01_07_425723 257 6 XL1-Blue XL1-Blue NNP 10_1101-2021_01_07_425723 257 7 was be VBD 10_1101-2021_01_07_425723 257 8 transformed transform VBN 10_1101-2021_01_07_425723 257 9 with with IN 10_1101-2021_01_07_425723 257 10 the the DT 10_1101-2021_01_07_425723 257 11 Gibson Gibson NNP 10_1101-2021_01_07_425723 257 12 assembly assembly NN 10_1101-2021_01_07_425723 257 13 mix mix NN 10_1101-2021_01_07_425723 257 14 via via IN 10_1101-2021_01_07_425723 257 15 a a DT 10_1101-2021_01_07_425723 257 16 5 5 CD 10_1101-2021_01_07_425723 257 17 min min NN 10_1101-2021_01_07_425723 257 18 incubation incubation NN 10_1101-2021_01_07_425723 257 19 on on IN 10_1101-2021_01_07_425723 257 20 ice ice NN 10_1101-2021_01_07_425723 257 21 followed follow VBN 10_1101-2021_01_07_425723 257 22 by by IN 10_1101-2021_01_07_425723 257 23 a a DT 10_1101-2021_01_07_425723 257 24 381 381 CD 10_1101-2021_01_07_425723 257 25 40 40 CD 10_1101-2021_01_07_425723 257 26 s s POS 10_1101-2021_01_07_425723 257 27 heat heat NN 10_1101-2021_01_07_425723 257 28 shock shock NN 10_1101-2021_01_07_425723 257 29 at at IN 10_1101-2021_01_07_425723 257 30 42 42 CD 10_1101-2021_01_07_425723 257 31 ° ° NNS 10_1101-2021_01_07_425723 257 32 C C NNP 10_1101-2021_01_07_425723 257 33 and and CC 10_1101-2021_01_07_425723 257 34 1 1 CD 10_1101-2021_01_07_425723 257 35 h h NN 10_1101-2021_01_07_425723 257 36 recovery recovery NN 10_1101-2021_01_07_425723 257 37 in in IN 10_1101-2021_01_07_425723 257 38 non non JJ 10_1101-2021_01_07_425723 257 39 - - JJ 10_1101-2021_01_07_425723 257 40 selective selective JJ 10_1101-2021_01_07_425723 257 41 LB LB NNP 10_1101-2021_01_07_425723 257 42 medium medium NN 10_1101-2021_01_07_425723 257 43 . . . 10_1101-2021_01_07_425723 258 1 Transformants transformant NNS 10_1101-2021_01_07_425723 258 2 were be VBD 10_1101-2021_01_07_425723 258 3 selected select VBN 10_1101-2021_01_07_425723 258 4 on on IN 10_1101-2021_01_07_425723 258 5 382 382 CD 10_1101-2021_01_07_425723 258 6 LB LB NNP 10_1101-2021_01_07_425723 258 7 agar agar NN 10_1101-2021_01_07_425723 258 8 containing contain VBG 10_1101-2021_01_07_425723 258 9 the the DT 10_1101-2021_01_07_425723 258 10 appropriate appropriate JJ 10_1101-2021_01_07_425723 258 11 antibiotic antibiotic NN 10_1101-2021_01_07_425723 258 12 . . . 10_1101-2021_01_07_425723 259 1 Golden Golden NNP 10_1101-2021_01_07_425723 259 2 Gate Gate NNP 10_1101-2021_01_07_425723 259 3 assembly assembly NN 10_1101-2021_01_07_425723 259 4 with with IN 10_1101-2021_01_07_425723 259 5 the the DT 10_1101-2021_01_07_425723 259 6 yeast yeast NN 10_1101-2021_01_07_425723 259 7 tool tool NN 10_1101-2021_01_07_425723 259 8 kit60 kit60 NNS 10_1101-2021_01_07_425723 259 9 was be VBD 10_1101-2021_01_07_425723 259 10 383 383 CD 10_1101-2021_01_07_425723 259 11 performed perform VBN 10_1101-2021_01_07_425723 259 12 in in IN 10_1101-2021_01_07_425723 259 13 20 20 CD 10_1101-2021_01_07_425723 259 14 µL µL NNP 10_1101-2021_01_07_425723 259 15 reaction reaction NN 10_1101-2021_01_07_425723 259 16 mixtures mixture NNS 10_1101-2021_01_07_425723 259 17 containing contain VBG 10_1101-2021_01_07_425723 259 18 0.75 0.75 CD 10_1101-2021_01_07_425723 259 19 µL µL . 10_1101-2021_01_07_425723 259 20 BsaI BsaI NNP 10_1101-2021_01_07_425723 259 21 HF HF NNP 10_1101-2021_01_07_425723 259 22 V2 V2 NNP 10_1101-2021_01_07_425723 259 23 ( ( -LRB- 10_1101-2021_01_07_425723 259 24 NEB NEB NNP 10_1101-2021_01_07_425723 259 25 , , , 10_1101-2021_01_07_425723 259 26 # # NNP 10_1101-2021_01_07_425723 259 27 R3733 R3733 NNP 10_1101-2021_01_07_425723 259 28 ) ) -RRB- 10_1101-2021_01_07_425723 259 29 , , , 10_1101-2021_01_07_425723 259 30 2 2 CD 10_1101-2021_01_07_425723 259 31 µL µL NNP 10_1101-2021_01_07_425723 259 32 DNA DNA NNP 10_1101-2021_01_07_425723 259 33 ligase ligase NN 10_1101-2021_01_07_425723 259 34 384 384 CD 10_1101-2021_01_07_425723 259 35 buffer buffer NN 10_1101-2021_01_07_425723 259 36 with with IN 10_1101-2021_01_07_425723 259 37 ATP ATP NNP 10_1101-2021_01_07_425723 259 38 ( ( -LRB- 10_1101-2021_01_07_425723 259 39 New New NNP 10_1101-2021_01_07_425723 259 40 England England NNP 10_1101-2021_01_07_425723 259 41 Biolabs Biolabs NNP 10_1101-2021_01_07_425723 259 42 ) ) -RRB- 10_1101-2021_01_07_425723 259 43 , , , 10_1101-2021_01_07_425723 259 44 0.5 0.5 CD 10_1101-2021_01_07_425723 259 45 µL µL NNP 10_1101-2021_01_07_425723 259 46 T7-ligase T7-ligase NNP 10_1101-2021_01_07_425723 259 47 ( ( -LRB- 10_1101-2021_01_07_425723 259 48 NEB NEB NNP 10_1101-2021_01_07_425723 259 49 ) ) -RRB- 10_1101-2021_01_07_425723 259 50 with with IN 10_1101-2021_01_07_425723 259 51 20 20 CD 10_1101-2021_01_07_425723 259 52 fmol fmol JJ 10_1101-2021_01_07_425723 259 53 DNA dna NN 10_1101-2021_01_07_425723 259 54 donor donor NN 10_1101-2021_01_07_425723 259 55 fragments fragment NNS 10_1101-2021_01_07_425723 259 56 and and CC 10_1101-2021_01_07_425723 259 57 385 385 CD 10_1101-2021_01_07_425723 259 58 MilliQ milliq NN 10_1101-2021_01_07_425723 259 59 water water NN 10_1101-2021_01_07_425723 259 60 . . . 10_1101-2021_01_07_425723 260 1 Before before IN 10_1101-2021_01_07_425723 260 2 ligation ligation NN 10_1101-2021_01_07_425723 260 3 at at IN 10_1101-2021_01_07_425723 260 4 16 16 CD 10_1101-2021_01_07_425723 260 5 ° ° NNS 10_1101-2021_01_07_425723 260 6 C C NNP 10_1101-2021_01_07_425723 260 7 was be VBD 10_1101-2021_01_07_425723 260 8 initiated initiate VBN 10_1101-2021_01_07_425723 260 9 by by IN 10_1101-2021_01_07_425723 260 10 addition addition NN 10_1101-2021_01_07_425723 260 11 of of IN 10_1101-2021_01_07_425723 260 12 T7 T7 NNP 10_1101-2021_01_07_425723 260 13 DNA DNA NNP 10_1101-2021_01_07_425723 260 14 ligase ligase NN 10_1101-2021_01_07_425723 260 15 , , , 10_1101-2021_01_07_425723 260 16 an an DT 10_1101-2021_01_07_425723 260 17 initial initial JJ 10_1101-2021_01_07_425723 260 18 BsaI BsaI NNP 10_1101-2021_01_07_425723 260 19 digestion digestion NN 10_1101-2021_01_07_425723 260 20 386 386 CD 10_1101-2021_01_07_425723 260 21 ( ( -LRB- 10_1101-2021_01_07_425723 260 22 30 30 CD 10_1101-2021_01_07_425723 260 23 min min NN 10_1101-2021_01_07_425723 260 24 at at IN 10_1101-2021_01_07_425723 260 25 37 37 CD 10_1101-2021_01_07_425723 260 26 ° ° , 10_1101-2021_01_07_425723 260 27 C C NNP 10_1101-2021_01_07_425723 260 28 ) ) -RRB- 10_1101-2021_01_07_425723 260 29 was be VBD 10_1101-2021_01_07_425723 260 30 performed perform VBN 10_1101-2021_01_07_425723 260 31 . . . 10_1101-2021_01_07_425723 261 1 Then then RB 10_1101-2021_01_07_425723 261 2 30 30 CD 10_1101-2021_01_07_425723 261 3 cycles cycle NNS 10_1101-2021_01_07_425723 261 4 of of IN 10_1101-2021_01_07_425723 261 5 digestion digestion NN 10_1101-2021_01_07_425723 261 6 and and CC 10_1101-2021_01_07_425723 261 7 ligation ligation NN 10_1101-2021_01_07_425723 261 8 at at IN 10_1101-2021_01_07_425723 261 9 37 37 CD 10_1101-2021_01_07_425723 261 10 ° ° NNS 10_1101-2021_01_07_425723 261 11 C C NNP 10_1101-2021_01_07_425723 261 12 and and CC 10_1101-2021_01_07_425723 261 13 16 16 CD 10_1101-2021_01_07_425723 261 14 ° ° , 10_1101-2021_01_07_425723 261 15 C C NNP 10_1101-2021_01_07_425723 261 16 , , , 10_1101-2021_01_07_425723 261 17 387 387 CD 10_1101-2021_01_07_425723 261 18 respectively respectively RB 10_1101-2021_01_07_425723 261 19 , , , 10_1101-2021_01_07_425723 261 20 were be VBD 10_1101-2021_01_07_425723 261 21 performed perform VBN 10_1101-2021_01_07_425723 261 22 , , , 10_1101-2021_01_07_425723 261 23 with with IN 10_1101-2021_01_07_425723 261 24 5 5 CD 10_1101-2021_01_07_425723 261 25 min min NN 10_1101-2021_01_07_425723 261 26 incubation incubation NN 10_1101-2021_01_07_425723 261 27 times time NNS 10_1101-2021_01_07_425723 261 28 for for IN 10_1101-2021_01_07_425723 261 29 each each DT 10_1101-2021_01_07_425723 261 30 reaction reaction NN 10_1101-2021_01_07_425723 261 31 . . . 10_1101-2021_01_07_425723 262 1 Thermocycling thermocycling NN 10_1101-2021_01_07_425723 262 2 was be VBD 10_1101-2021_01_07_425723 262 3 388 388 CD 10_1101-2021_01_07_425723 262 4 terminated terminate VBN 10_1101-2021_01_07_425723 262 5 with with IN 10_1101-2021_01_07_425723 262 6 a a DT 10_1101-2021_01_07_425723 262 7 5 5 CD 10_1101-2021_01_07_425723 262 8 min min NN 10_1101-2021_01_07_425723 262 9 final final JJ 10_1101-2021_01_07_425723 262 10 digestion digestion NN 10_1101-2021_01_07_425723 262 11 step step NN 10_1101-2021_01_07_425723 262 12 at at IN 10_1101-2021_01_07_425723 262 13 60 60 CD 10_1101-2021_01_07_425723 262 14 ° ° NNS 10_1101-2021_01_07_425723 262 15 C C NNP 10_1101-2021_01_07_425723 262 16 . . . 10_1101-2021_01_07_425723 263 1 389 389 CD 10_1101-2021_01_07_425723 263 2 To to TO 10_1101-2021_01_07_425723 263 3 construct construct VB 10_1101-2021_01_07_425723 263 4 a a DT 10_1101-2021_01_07_425723 263 5 TtSTC1 ttstc1 CD 10_1101-2021_01_07_425723 263 6 expression expression NN 10_1101-2021_01_07_425723 263 7 vector vector NN 10_1101-2021_01_07_425723 263 8 , , , 10_1101-2021_01_07_425723 263 9 the the DT 10_1101-2021_01_07_425723 263 10 coding code VBG 10_1101-2021_01_07_425723 263 11 sequence sequence NN 10_1101-2021_01_07_425723 263 12 of of IN 10_1101-2021_01_07_425723 263 13 TtSTC1 ttstc1 NN 10_1101-2021_01_07_425723 263 14 ( ( -LRB- 10_1101-2021_01_07_425723 263 15 pUD696 pUD696 NNP 10_1101-2021_01_07_425723 263 16 ) ) -RRB- 10_1101-2021_01_07_425723 263 17 was be VBD 10_1101-2021_01_07_425723 263 18 PCR PCR NNP 10_1101-2021_01_07_425723 263 19 amplified amplify VBD 10_1101-2021_01_07_425723 263 20 390 390 CD 10_1101-2021_01_07_425723 263 21 with with IN 10_1101-2021_01_07_425723 263 22 primer primer NNP 10_1101-2021_01_07_425723 263 23 pair pair NNP 10_1101-2021_01_07_425723 263 24 16096/16097 16096/16097 NNP 10_1101-2021_01_07_425723 263 25 and and CC 10_1101-2021_01_07_425723 263 26 Golden golden JJ 10_1101-2021_01_07_425723 263 27 gate gate NN 10_1101-2021_01_07_425723 263 28 assembled assemble VBN 10_1101-2021_01_07_425723 263 29 with with IN 10_1101-2021_01_07_425723 263 30 the the DT 10_1101-2021_01_07_425723 263 31 donor donor NN 10_1101-2021_01_07_425723 263 32 plasmids plasmid NNS 10_1101-2021_01_07_425723 263 33 pGGkd015 pggkd015 CD 10_1101-2021_01_07_425723 263 34 ( ( -LRB- 10_1101-2021_01_07_425723 263 35 ori ori RB 10_1101-2021_01_07_425723 263 36 391 391 CD 10_1101-2021_01_07_425723 263 37 ampR ampr CD 10_1101-2021_01_07_425723 263 38 ) ) -RRB- 10_1101-2021_01_07_425723 263 39 , , , 10_1101-2021_01_07_425723 263 40 pP2 pp2 NN 10_1101-2021_01_07_425723 263 41 ( ( -LRB- 10_1101-2021_01_07_425723 263 42 KmPDC1p KmPDC1p NNP 10_1101-2021_01_07_425723 263 43 ) ) -RRB- 10_1101-2021_01_07_425723 263 44 , , , 10_1101-2021_01_07_425723 263 45 pYTK053 pytk053 NN 10_1101-2021_01_07_425723 263 46 ( ( -LRB- 10_1101-2021_01_07_425723 263 47 ScADH1 scadh1 CD 10_1101-2021_01_07_425723 263 48 t t NNP 10_1101-2021_01_07_425723 263 49 ) ) -RRB- 10_1101-2021_01_07_425723 263 50 resulting result VBG 10_1101-2021_01_07_425723 263 51 in in IN 10_1101-2021_01_07_425723 263 52 pUDE909 pUDE909 NNP 10_1101-2021_01_07_425723 263 53 ( ( -LRB- 10_1101-2021_01_07_425723 263 54 ori ori NN 10_1101-2021_01_07_425723 263 55 ampR ampR NNP 10_1101-2021_01_07_425723 263 56 KmPDC1p KmPDC1p NNP 10_1101-2021_01_07_425723 263 57 - - HYPH 10_1101-2021_01_07_425723 263 58 TtSTC1 ttstc1 CD 10_1101-2021_01_07_425723 263 59 - - HYPH 10_1101-2021_01_07_425723 263 60 392 392 CD 10_1101-2021_01_07_425723 263 61 ScADH1 scadh1 CD 10_1101-2021_01_07_425723 263 62 t t NN 10_1101-2021_01_07_425723 263 63 ) ) -RRB- 10_1101-2021_01_07_425723 263 64 . . . 10_1101-2021_01_07_425723 264 1 For for IN 10_1101-2021_01_07_425723 264 2 integration integration NN 10_1101-2021_01_07_425723 264 3 of of IN 10_1101-2021_01_07_425723 264 4 TtSTC1 ttstc1 JJ 10_1101-2021_01_07_425723 264 5 cassette cassette NN 10_1101-2021_01_07_425723 264 6 into into IN 10_1101-2021_01_07_425723 264 7 the the DT 10_1101-2021_01_07_425723 264 8 lac4 lac4 NNP 10_1101-2021_01_07_425723 264 9 locus locus NNP 10_1101-2021_01_07_425723 264 10 both both DT 10_1101-2021_01_07_425723 264 11 upstream upstream NNP 10_1101-2021_01_07_425723 264 12 and and CC 10_1101-2021_01_07_425723 264 13 downstream downstream JJ 10_1101-2021_01_07_425723 264 14 flanks flank VBZ 10_1101-2021_01_07_425723 264 15 393 393 CD 10_1101-2021_01_07_425723 264 16 ( ( -LRB- 10_1101-2021_01_07_425723 264 17 877/878 877/878 NNP 10_1101-2021_01_07_425723 264 18 bps bps NNP 10_1101-2021_01_07_425723 264 19 ) ) -RRB- 10_1101-2021_01_07_425723 264 20 of of IN 10_1101-2021_01_07_425723 264 21 the the DT 10_1101-2021_01_07_425723 264 22 lac4 lac4 NNP 10_1101-2021_01_07_425723 264 23 locus locus NN 10_1101-2021_01_07_425723 264 24 were be VBD 10_1101-2021_01_07_425723 264 25 PCR PCR NNP 10_1101-2021_01_07_425723 264 26 amplified amplify VBN 10_1101-2021_01_07_425723 264 27 with with IN 10_1101-2021_01_07_425723 264 28 the the DT 10_1101-2021_01_07_425723 264 29 primer primer NNP 10_1101-2021_01_07_425723 264 30 pairs pairs NNP 10_1101-2021_01_07_425723 264 31 14197/14198 14197/14198 CD 10_1101-2021_01_07_425723 264 32 and and CC 10_1101-2021_01_07_425723 264 33 394 394 CD 10_1101-2021_01_07_425723 264 34 14199/14200 14199/14200 CD 10_1101-2021_01_07_425723 264 35 , , , 10_1101-2021_01_07_425723 264 36 respectively respectively RB 10_1101-2021_01_07_425723 264 37 . . . 10_1101-2021_01_07_425723 265 1 An an DT 10_1101-2021_01_07_425723 265 2 empty empty JJ 10_1101-2021_01_07_425723 265 3 integration integration NN 10_1101-2021_01_07_425723 265 4 vector vector NN 10_1101-2021_01_07_425723 265 5 , , , 10_1101-2021_01_07_425723 265 6 pGGKd068 pGGKd068 NNP 10_1101-2021_01_07_425723 265 7 , , , 10_1101-2021_01_07_425723 265 8 was be VBD 10_1101-2021_01_07_425723 265 9 constructed construct VBN 10_1101-2021_01_07_425723 265 10 by by IN 10_1101-2021_01_07_425723 265 11 BsaI BsaI NNP 10_1101-2021_01_07_425723 265 12 golden golden JJ 10_1101-2021_01_07_425723 265 13 395 395 CD 10_1101-2021_01_07_425723 265 14 gate gate NN 10_1101-2021_01_07_425723 265 15 cloning cloning NN 10_1101-2021_01_07_425723 265 16 of of IN 10_1101-2021_01_07_425723 265 17 pYTK047 pYTK047 NNP 10_1101-2021_01_07_425723 265 18 ( ( -LRB- 10_1101-2021_01_07_425723 265 19 GFP GFP NNP 10_1101-2021_01_07_425723 265 20 - - HYPH 10_1101-2021_01_07_425723 265 21 dropout dropout NNP 10_1101-2021_01_07_425723 265 22 ) ) -RRB- 10_1101-2021_01_07_425723 265 23 , , , 10_1101-2021_01_07_425723 265 24 pYTK079 pYTK079 NNP 10_1101-2021_01_07_425723 265 25 ( ( -LRB- 10_1101-2021_01_07_425723 265 26 hygB hygB NNP 10_1101-2021_01_07_425723 265 27 ) ) -RRB- 10_1101-2021_01_07_425723 265 28 , , , 10_1101-2021_01_07_425723 265 29 pYTK090 pYTK090 NNP 10_1101-2021_01_07_425723 265 30 ( ( -LRB- 10_1101-2021_01_07_425723 265 31 kanR kanR NNP 10_1101-2021_01_07_425723 265 32 ) ) -RRB- 10_1101-2021_01_07_425723 265 33 , , , 10_1101-2021_01_07_425723 265 34 pYTK073 pYTK073 NNP 10_1101-2021_01_07_425723 265 35 ( ( -LRB- 10_1101-2021_01_07_425723 265 36 ConRE ConRE NNP 10_1101-2021_01_07_425723 265 37 ’ ' '' 10_1101-2021_01_07_425723 265 38 ) ) -RRB- 10_1101-2021_01_07_425723 265 39 , , , 10_1101-2021_01_07_425723 265 40 pYTK008 pYTK008 NNP 10_1101-2021_01_07_425723 265 41 396 396 CD 10_1101-2021_01_07_425723 265 42 ( ( -LRB- 10_1101-2021_01_07_425723 265 43 ConLS ConLS NNP 10_1101-2021_01_07_425723 265 44 ’ ' '' 10_1101-2021_01_07_425723 265 45 ) ) -RRB- 10_1101-2021_01_07_425723 265 46 together together RB 10_1101-2021_01_07_425723 265 47 with with IN 10_1101-2021_01_07_425723 265 48 the the DT 10_1101-2021_01_07_425723 265 49 two two CD 10_1101-2021_01_07_425723 265 50 lac4 lac4 NN 10_1101-2021_01_07_425723 265 51 homologous homologous JJ 10_1101-2021_01_07_425723 265 52 nucleotide nucleotide JJ 10_1101-2021_01_07_425723 265 53 sequences sequence NNS 10_1101-2021_01_07_425723 265 54 . . . 10_1101-2021_01_07_425723 266 1 Plasmid Plasmid NNP 10_1101-2021_01_07_425723 266 2 assembly assembly NN 10_1101-2021_01_07_425723 266 3 was be VBD 10_1101-2021_01_07_425723 266 4 verified verify VBN 10_1101-2021_01_07_425723 266 5 397 397 CD 10_1101-2021_01_07_425723 266 6 by by IN 10_1101-2021_01_07_425723 266 7 PCR PCR NNP 10_1101-2021_01_07_425723 266 8 amplification amplification NN 10_1101-2021_01_07_425723 266 9 with with IN 10_1101-2021_01_07_425723 266 10 primers primer NNS 10_1101-2021_01_07_425723 266 11 15210 15210 CD 10_1101-2021_01_07_425723 266 12 , , , 10_1101-2021_01_07_425723 266 13 9335 9335 CD 10_1101-2021_01_07_425723 266 14 , , , 10_1101-2021_01_07_425723 266 15 16274 16274 CD 10_1101-2021_01_07_425723 266 16 and and CC 10_1101-2021_01_07_425723 266 17 16275 16275 CD 10_1101-2021_01_07_425723 266 18 and and CC 10_1101-2021_01_07_425723 266 19 by by IN 10_1101-2021_01_07_425723 266 20 digestion digestion NN 10_1101-2021_01_07_425723 266 21 with with IN 10_1101-2021_01_07_425723 266 22 BsmBI BsmBI NNP 10_1101-2021_01_07_425723 266 23 ( ( -LRB- 10_1101-2021_01_07_425723 266 24 New New NNP 10_1101-2021_01_07_425723 266 25 398 398 CD 10_1101-2021_01_07_425723 266 26 England England NNP 10_1101-2021_01_07_425723 266 27 Biolabs Biolabs NNP 10_1101-2021_01_07_425723 266 28 , , , 10_1101-2021_01_07_425723 266 29 # # NNP 10_1101-2021_01_07_425723 266 30 R0580 R0580 NNP 10_1101-2021_01_07_425723 266 31 ) ) -RRB- 10_1101-2021_01_07_425723 266 32 . . . 10_1101-2021_01_07_425723 267 1 The the DT 10_1101-2021_01_07_425723 267 2 integration integration NN 10_1101-2021_01_07_425723 267 3 vector vector NN 10_1101-2021_01_07_425723 267 4 pUDI246 pUDI246 NNP 10_1101-2021_01_07_425723 267 5 with with IN 10_1101-2021_01_07_425723 267 6 the the DT 10_1101-2021_01_07_425723 267 7 TtSTC1 ttstc1 JJ 10_1101-2021_01_07_425723 267 8 expression expression NN 10_1101-2021_01_07_425723 267 9 cassette cassette NN 10_1101-2021_01_07_425723 267 10 was be VBD 10_1101-2021_01_07_425723 267 11 399 399 CD 10_1101-2021_01_07_425723 267 12 constructed construct VBN 10_1101-2021_01_07_425723 267 13 by by IN 10_1101-2021_01_07_425723 267 14 Gibson Gibson NNP 10_1101-2021_01_07_425723 267 15 assembly assembly NN 10_1101-2021_01_07_425723 267 16 of of IN 10_1101-2021_01_07_425723 267 17 the the DT 10_1101-2021_01_07_425723 267 18 PCR PCR NNP 10_1101-2021_01_07_425723 267 19 amplified amplify VBD 10_1101-2021_01_07_425723 267 20 pGGKd068 pGGKd068 NNP 10_1101-2021_01_07_425723 267 21 and and CC 10_1101-2021_01_07_425723 267 22 pUDE909 pUDE909 NNPS 10_1101-2021_01_07_425723 267 23 with with IN 10_1101-2021_01_07_425723 267 24 primer primer NNP 10_1101-2021_01_07_425723 267 25 pairs pairs NNP 10_1101-2021_01_07_425723 267 26 400 400 CD 10_1101-2021_01_07_425723 267 27 16274/16275 16274/16275 CD 10_1101-2021_01_07_425723 267 28 and and CC 10_1101-2021_01_07_425723 267 29 16272/16273 16272/16273 CD 10_1101-2021_01_07_425723 267 30 , , , 10_1101-2021_01_07_425723 267 31 thereby thereby RB 10_1101-2021_01_07_425723 267 32 adding add VBG 10_1101-2021_01_07_425723 267 33 20 20 CD 10_1101-2021_01_07_425723 267 34 bp bp NN 10_1101-2021_01_07_425723 267 35 overlaps overlap NNS 10_1101-2021_01_07_425723 267 36 for for IN 10_1101-2021_01_07_425723 267 37 assembly assembly NN 10_1101-2021_01_07_425723 267 38 . . . 10_1101-2021_01_07_425723 268 1 For for IN 10_1101-2021_01_07_425723 268 2 this this DT 10_1101-2021_01_07_425723 268 3 step step NN 10_1101-2021_01_07_425723 268 4 , , , 10_1101-2021_01_07_425723 268 5 the the DT 10_1101-2021_01_07_425723 268 6 401 401 CD 10_1101-2021_01_07_425723 268 7 .CC .CC : 10_1101-2021_01_07_425723 268 8 - - HYPH 10_1101-2021_01_07_425723 268 9 BY by IN 10_1101-2021_01_07_425723 268 10 - - HYPH 10_1101-2021_01_07_425723 268 11 NC NC NNP 10_1101-2021_01_07_425723 268 12 - - HYPH 10_1101-2021_01_07_425723 268 13 ND ND NNP 10_1101-2021_01_07_425723 268 14 4.0 4.0 CD 10_1101-2021_01_07_425723 268 15 International International NNP 10_1101-2021_01_07_425723 268 16 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 268 17 under under IN 10_1101-2021_01_07_425723 268 18 a a DT 10_1101-2021_01_07_425723 268 19 ( ( -LRB- 10_1101-2021_01_07_425723 268 20 which which WDT 10_1101-2021_01_07_425723 268 21 was be VBD 10_1101-2021_01_07_425723 268 22 not not RB 10_1101-2021_01_07_425723 268 23 certified certify VBN 10_1101-2021_01_07_425723 268 24 by by IN 10_1101-2021_01_07_425723 268 25 peer peer NN 10_1101-2021_01_07_425723 268 26 review review NN 10_1101-2021_01_07_425723 268 27 ) ) -RRB- 10_1101-2021_01_07_425723 268 28 is be VBZ 10_1101-2021_01_07_425723 268 29 the the DT 10_1101-2021_01_07_425723 268 30 author author NN 10_1101-2021_01_07_425723 268 31 / / SYM 10_1101-2021_01_07_425723 268 32 funder funder NN 10_1101-2021_01_07_425723 268 33 , , , 10_1101-2021_01_07_425723 268 34 who who WP 10_1101-2021_01_07_425723 268 35 has have VBZ 10_1101-2021_01_07_425723 268 36 granted grant VBN 10_1101-2021_01_07_425723 268 37 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 268 38 a a DT 10_1101-2021_01_07_425723 268 39 license license NN 10_1101-2021_01_07_425723 268 40 to to TO 10_1101-2021_01_07_425723 268 41 display display VB 10_1101-2021_01_07_425723 268 42 the the DT 10_1101-2021_01_07_425723 268 43 preprint preprint NN 10_1101-2021_01_07_425723 268 44 in in IN 10_1101-2021_01_07_425723 268 45 perpetuity perpetuity NN 10_1101-2021_01_07_425723 268 46 . . . 10_1101-2021_01_07_425723 269 1 It -PRON- PRP 10_1101-2021_01_07_425723 269 2 is be VBZ 10_1101-2021_01_07_425723 269 3 made make VBN 10_1101-2021_01_07_425723 269 4 The the DT 10_1101-2021_01_07_425723 269 5 copyright copyright NN 10_1101-2021_01_07_425723 269 6 holder holder NN 10_1101-2021_01_07_425723 269 7 for for IN 10_1101-2021_01_07_425723 269 8 this this DT 10_1101-2021_01_07_425723 269 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 269 10 version version NN 10_1101-2021_01_07_425723 269 11 posted post VBD 10_1101-2021_01_07_425723 269 12 January January NNP 10_1101-2021_01_07_425723 269 13 8 8 CD 10_1101-2021_01_07_425723 269 14 , , , 10_1101-2021_01_07_425723 269 15 2021 2021 CD 10_1101-2021_01_07_425723 269 16 . . . 10_1101-2021_01_07_425723 269 17 ; ; : 10_1101-2021_01_07_425723 269 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 269 19 : : : 10_1101-2021_01_07_425723 269 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 269 21 preprint preprint NN 10_1101-2021_01_07_425723 269 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 269 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 269 24 24 24 CD 10_1101-2021_01_07_425723 269 25 incubation incubation NN 10_1101-2021_01_07_425723 269 26 time time NN 10_1101-2021_01_07_425723 269 27 of of IN 10_1101-2021_01_07_425723 269 28 the the DT 10_1101-2021_01_07_425723 269 29 Gibson Gibson NNP 10_1101-2021_01_07_425723 269 30 assembly assembly NN 10_1101-2021_01_07_425723 269 31 was be VBD 10_1101-2021_01_07_425723 269 32 increased increase VBN 10_1101-2021_01_07_425723 269 33 to to IN 10_1101-2021_01_07_425723 269 34 90 90 CD 10_1101-2021_01_07_425723 269 35 min min NN 10_1101-2021_01_07_425723 269 36 . . . 10_1101-2021_01_07_425723 270 1 Plasmid Plasmid NNP 10_1101-2021_01_07_425723 270 2 assembly assembly NN 10_1101-2021_01_07_425723 270 3 was be VBD 10_1101-2021_01_07_425723 270 4 verified verify VBN 10_1101-2021_01_07_425723 270 5 by by IN 10_1101-2021_01_07_425723 270 6 402 402 CD 10_1101-2021_01_07_425723 270 7 diagnostic diagnostic JJ 10_1101-2021_01_07_425723 270 8 PCR PCR NNP 10_1101-2021_01_07_425723 270 9 amplification amplification NN 10_1101-2021_01_07_425723 270 10 using use VBG 10_1101-2021_01_07_425723 270 11 DreamTaq DreamTaq NNP 10_1101-2021_01_07_425723 270 12 polymerase polymerase NN 10_1101-2021_01_07_425723 270 13 ( ( -LRB- 10_1101-2021_01_07_425723 270 14 Thermo Thermo NNP 10_1101-2021_01_07_425723 270 15 Scientific Scientific NNP 10_1101-2021_01_07_425723 270 16 ) ) -RRB- 10_1101-2021_01_07_425723 270 17 with with IN 10_1101-2021_01_07_425723 270 18 primers primer NNS 10_1101-2021_01_07_425723 270 19 5941 5941 CD 10_1101-2021_01_07_425723 270 20 , , , 10_1101-2021_01_07_425723 270 21 8442 8442 CD 10_1101-2021_01_07_425723 270 22 , , , 10_1101-2021_01_07_425723 270 23 403 403 CD 10_1101-2021_01_07_425723 270 24 15216 15216 CD 10_1101-2021_01_07_425723 270 25 and and CC 10_1101-2021_01_07_425723 270 26 subsequent subsequent JJ 10_1101-2021_01_07_425723 270 27 Illumina Illumina NNP 10_1101-2021_01_07_425723 270 28 short short JJ 10_1101-2021_01_07_425723 270 29 - - HYPH 10_1101-2021_01_07_425723 270 30 read read NN 10_1101-2021_01_07_425723 270 31 sequencing sequencing NN 10_1101-2021_01_07_425723 270 32 . . . 10_1101-2021_01_07_425723 271 1 404 404 CD 10_1101-2021_01_07_425723 271 2 Table table NN 10_1101-2021_01_07_425723 271 3 2 2 CD 10_1101-2021_01_07_425723 271 4 | | NNP 10_1101-2021_01_07_425723 271 5 Strains Strains NNP 10_1101-2021_01_07_425723 271 6 used use VBN 10_1101-2021_01_07_425723 271 7 in in IN 10_1101-2021_01_07_425723 271 8 this this DT 10_1101-2021_01_07_425723 271 9 study study NN 10_1101-2021_01_07_425723 271 10 . . . 10_1101-2021_01_07_425723 272 1 Abbreviations abbreviation NNS 10_1101-2021_01_07_425723 272 2 : : : 10_1101-2021_01_07_425723 272 3 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 272 4 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 272 5 ( ( -LRB- 10_1101-2021_01_07_425723 272 6 Sc Sc NNP 10_1101-2021_01_07_425723 272 7 ) ) -RRB- 10_1101-2021_01_07_425723 272 8 , , , 10_1101-2021_01_07_425723 272 9 Kluyveromyces kluyveromyce NNS 10_1101-2021_01_07_425723 272 10 405 405 CD 10_1101-2021_01_07_425723 272 11 marxianus marxianu NNS 10_1101-2021_01_07_425723 272 12 ( ( -LRB- 10_1101-2021_01_07_425723 272 13 Km Km NNP 10_1101-2021_01_07_425723 272 14 ) ) -RRB- 10_1101-2021_01_07_425723 272 15 , , , 10_1101-2021_01_07_425723 272 16 Tetrahymena Tetrahymena NNP 10_1101-2021_01_07_425723 272 17 thermophila thermophila NN 10_1101-2021_01_07_425723 272 18 ( ( -LRB- 10_1101-2021_01_07_425723 272 19 Tt Tt NNP 10_1101-2021_01_07_425723 272 20 ) ) -RRB- 10_1101-2021_01_07_425723 272 21 . . . 10_1101-2021_01_07_425723 273 1 406 406 CD 10_1101-2021_01_07_425723 273 2 Genus Genus NNP 10_1101-2021_01_07_425723 273 3 Strain Strain NNP 10_1101-2021_01_07_425723 273 4 Relevant relevant JJ 10_1101-2021_01_07_425723 273 5 genotype genotype NN 10_1101-2021_01_07_425723 273 6 Reference Reference NNP 10_1101-2021_01_07_425723 273 7 S. S. NNP 10_1101-2021_01_07_425723 273 8 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 273 9 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 273 10 - - HYPH 10_1101-2021_01_07_425723 273 11 7D 7d NN 10_1101-2021_01_07_425723 273 12 MATa MATa NNS 10_1101-2021_01_07_425723 273 13 URA3 URA3 NNP 10_1101-2021_01_07_425723 273 14 HIS3 HIS3 NNP 10_1101-2021_01_07_425723 273 15 LEU2 LEU2 NNP 10_1101-2021_01_07_425723 273 16 TRP1 TRP1 NNP 10_1101-2021_01_07_425723 273 17 MAL2 MAL2 NNP 10_1101-2021_01_07_425723 273 18 - - HYPH 10_1101-2021_01_07_425723 273 19 8c 8c NNP 10_1101-2021_01_07_425723 273 20 SUC2 SUC2 NNP 10_1101-2021_01_07_425723 273 21 Entian Entian NNP 10_1101-2021_01_07_425723 273 22 and and CC 10_1101-2021_01_07_425723 273 23 Kötter Kötter NNP 10_1101-2021_01_07_425723 273 24 , , , 10_1101-2021_01_07_425723 273 25 2007 2007 CD 10_1101-2021_01_07_425723 273 26 57 57 CD 10_1101-2021_01_07_425723 273 27 S. s. NN 10_1101-2021_01_07_425723 273 28 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 273 29 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 273 30 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 273 31 - - HYPH 10_1101-2021_01_07_425723 273 32 7D 7d NN 10_1101-2021_01_07_425723 273 33 can1Δ::cas9-natNT2 can1Δ::cas9-natNT2 NNPS 10_1101-2021_01_07_425723 273 34 Mans Mans NNPS 10_1101-2021_01_07_425723 273 35 et et NNP 10_1101-2021_01_07_425723 273 36 al al NNP 10_1101-2021_01_07_425723 273 37 . . NNP 10_1101-2021_01_07_425723 273 38 , , , 10_1101-2021_01_07_425723 273 39 2015 2015 CD 10_1101-2021_01_07_425723 273 40 61 61 CD 10_1101-2021_01_07_425723 273 41 S. S. NNP 10_1101-2021_01_07_425723 273 42 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 273 43 IMX1438 IMX1438 NNP 10_1101-2021_01_07_425723 273 44 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 273 45 sga1Δ::TtSTC1 sga1Δ::TtSTC1 NNP 10_1101-2021_01_07_425723 273 46 Wiersma Wiersma NNP 10_1101-2021_01_07_425723 273 47 et et NNP 10_1101-2021_01_07_425723 273 48 al al NNP 10_1101-2021_01_07_425723 273 49 . . NNP 10_1101-2021_01_07_425723 273 50 , , , 10_1101-2021_01_07_425723 273 51 2020 2020 CD 10_1101-2021_01_07_425723 273 52 46 46 CD 10_1101-2021_01_07_425723 273 53 S. S. NNP 10_1101-2021_01_07_425723 273 54 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 273 55 IMK802 IMK802 NNP 10_1101-2021_01_07_425723 273 56 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 273 57 aus1Δ aus1Δ NNP 10_1101-2021_01_07_425723 273 58 This this DT 10_1101-2021_01_07_425723 273 59 study study NN 10_1101-2021_01_07_425723 273 60 S. S. NNP 10_1101-2021_01_07_425723 273 61 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 273 62 IMK806 IMK806 NNP 10_1101-2021_01_07_425723 273 63 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 273 64 pdr11Δ pdr11Δ NNP 10_1101-2021_01_07_425723 273 65 This this DT 10_1101-2021_01_07_425723 273 66 study study NN 10_1101-2021_01_07_425723 273 67 S. S. NNP 10_1101-2021_01_07_425723 273 68 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 273 69 IMK809 IMK809 NNP 10_1101-2021_01_07_425723 273 70 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 273 71 aus1Δ aus1Δ NNP 10_1101-2021_01_07_425723 273 72 pdr11Δ pdr11Δ NNP 10_1101-2021_01_07_425723 273 73 This this DT 10_1101-2021_01_07_425723 273 74 study study NN 10_1101-2021_01_07_425723 273 75 K. K. NNP 10_1101-2021_01_07_425723 273 76 marxianus marxianus NN 10_1101-2021_01_07_425723 273 77 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 273 78 URA3 URA3 NNP 10_1101-2021_01_07_425723 273 79 HIS3 HIS3 NNP 10_1101-2021_01_07_425723 273 80 LEU2 LEU2 NNP 10_1101-2021_01_07_425723 273 81 TRP1 TRP1 NNP 10_1101-2021_01_07_425723 273 82 CBS CBS NNP 10_1101-2021_01_07_425723 273 83 - - HYPH 10_1101-2021_01_07_425723 273 84 KNAW KNAW NNP 10_1101-2021_01_07_425723 273 85 * * NFP 10_1101-2021_01_07_425723 273 86 K. K. NNP 10_1101-2021_01_07_425723 273 87 marxianus marxianus NN 10_1101-2021_01_07_425723 273 88 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 273 89 URA3 URA3 NNP 10_1101-2021_01_07_425723 273 90 HIS3 HIS3 NNP 10_1101-2021_01_07_425723 273 91 LEU2 LEU2 NNP 10_1101-2021_01_07_425723 273 92 TRP1 TRP1 NNP 10_1101-2021_01_07_425723 273 93 NBRC NBRC NNP 10_1101-2021_01_07_425723 273 94 * * NFP 10_1101-2021_01_07_425723 273 95 * * NFP 10_1101-2021_01_07_425723 273 96 K. K. NNP 10_1101-2021_01_07_425723 273 97 marxianus marxianus NN 10_1101-2021_01_07_425723 273 98 IMX2323 IMX2323 NNP 10_1101-2021_01_07_425723 273 99 KmPDC1p KmPDC1p NNP 10_1101-2021_01_07_425723 273 100 - - HYPH 10_1101-2021_01_07_425723 273 101 TtSTC1-ScADH1t TtSTC1-ScADH1t NNP 10_1101-2021_01_07_425723 273 102 - - HYPH 10_1101-2021_01_07_425723 273 103 hygB hygB NNP 10_1101-2021_01_07_425723 273 104 This this DT 10_1101-2021_01_07_425723 273 105 study study NN 10_1101-2021_01_07_425723 273 106 K. K. NNP 10_1101-2021_01_07_425723 273 107 marxianus marxianus NN 10_1101-2021_01_07_425723 273 108 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 273 109 KmPDC1p KmPDC1p NNP 10_1101-2021_01_07_425723 273 110 - - HYPH 10_1101-2021_01_07_425723 273 111 TtSTC1-ScADH1t TtSTC1-ScADH1t NNP 10_1101-2021_01_07_425723 273 112 - - HYPH 10_1101-2021_01_07_425723 273 113 hygB hygB NNP 10_1101-2021_01_07_425723 273 114 This this DT 10_1101-2021_01_07_425723 273 115 study study NN 10_1101-2021_01_07_425723 273 116 K. K. NNP 10_1101-2021_01_07_425723 273 117 marxianus marxianus NN 10_1101-2021_01_07_425723 273 118 IMS1112 IMS1112 NNP 10_1101-2021_01_07_425723 273 119 KmPDC1p KmPDC1p NNP 10_1101-2021_01_07_425723 273 120 - - HYPH 10_1101-2021_01_07_425723 273 121 TtSTC1-ScADH1t TtSTC1-ScADH1t NNP 10_1101-2021_01_07_425723 273 122 - - HYPH 10_1101-2021_01_07_425723 273 123 hygB hygB NNP 10_1101-2021_01_07_425723 273 124 This this DT 10_1101-2021_01_07_425723 273 125 study study NN 10_1101-2021_01_07_425723 273 126 K. K. NNP 10_1101-2021_01_07_425723 273 127 marxianus marxianus NN 10_1101-2021_01_07_425723 273 128 IMS1113 IMS1113 NNP 10_1101-2021_01_07_425723 273 129 KmPDC1p KmPDC1p NNP 10_1101-2021_01_07_425723 273 130 - - HYPH 10_1101-2021_01_07_425723 273 131 TtSTC1-ScADH1t TtSTC1-ScADH1t NNP 10_1101-2021_01_07_425723 273 132 - - HYPH 10_1101-2021_01_07_425723 273 133 hygB hygB NNP 10_1101-2021_01_07_425723 273 134 This this DT 10_1101-2021_01_07_425723 273 135 study study NN 10_1101-2021_01_07_425723 273 136 K. K. NNP 10_1101-2021_01_07_425723 273 137 marxianus marxianus NN 10_1101-2021_01_07_425723 273 138 IMS1131 IMS1131 NNP 10_1101-2021_01_07_425723 273 139 KmPDC1p KmPDC1p NNP 10_1101-2021_01_07_425723 273 140 - - HYPH 10_1101-2021_01_07_425723 273 141 TtSTC1-ScADH1t TtSTC1-ScADH1t NNP 10_1101-2021_01_07_425723 273 142 - - HYPH 10_1101-2021_01_07_425723 273 143 hygB hygB NNP 10_1101-2021_01_07_425723 273 144 This this DT 10_1101-2021_01_07_425723 273 145 study study NN 10_1101-2021_01_07_425723 273 146 K. K. NNP 10_1101-2021_01_07_425723 273 147 marxianus marxianus NN 10_1101-2021_01_07_425723 273 148 IMS1132 IMS1132 NNP 10_1101-2021_01_07_425723 273 149 KmPDC1p KmPDC1p NNP 10_1101-2021_01_07_425723 273 150 - - HYPH 10_1101-2021_01_07_425723 273 151 TtSTC1-ScADH1t TtSTC1-ScADH1t NNP 10_1101-2021_01_07_425723 273 152 - - HYPH 10_1101-2021_01_07_425723 273 153 hygB hygB NNP 10_1101-2021_01_07_425723 273 154 This this DT 10_1101-2021_01_07_425723 273 155 study study NN 10_1101-2021_01_07_425723 273 156 K. K. NNP 10_1101-2021_01_07_425723 273 157 marxianus marxianus NN 10_1101-2021_01_07_425723 273 158 IMS1133 IMS1133 NNP 10_1101-2021_01_07_425723 273 159 KmPDC1p KmPDC1p NNP 10_1101-2021_01_07_425723 273 160 - - HYPH 10_1101-2021_01_07_425723 273 161 TtSTC1-ScADH1t TtSTC1-ScADH1t NNP 10_1101-2021_01_07_425723 273 162 - - HYPH 10_1101-2021_01_07_425723 273 163 hygB hygB NNP 10_1101-2021_01_07_425723 273 164 This this DT 10_1101-2021_01_07_425723 273 165 study study NN 10_1101-2021_01_07_425723 273 166 407 407 CD 10_1101-2021_01_07_425723 273 167 .CC .CC , 10_1101-2021_01_07_425723 273 168 - - HYPH 10_1101-2021_01_07_425723 273 169 BY by IN 10_1101-2021_01_07_425723 273 170 - - HYPH 10_1101-2021_01_07_425723 273 171 NC NC NNP 10_1101-2021_01_07_425723 273 172 - - HYPH 10_1101-2021_01_07_425723 273 173 ND ND NNP 10_1101-2021_01_07_425723 273 174 4.0 4.0 CD 10_1101-2021_01_07_425723 273 175 International International NNP 10_1101-2021_01_07_425723 273 176 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 273 177 under under IN 10_1101-2021_01_07_425723 273 178 a a DT 10_1101-2021_01_07_425723 273 179 ( ( -LRB- 10_1101-2021_01_07_425723 273 180 which which WDT 10_1101-2021_01_07_425723 273 181 was be VBD 10_1101-2021_01_07_425723 273 182 not not RB 10_1101-2021_01_07_425723 273 183 certified certify VBN 10_1101-2021_01_07_425723 273 184 by by IN 10_1101-2021_01_07_425723 273 185 peer peer NN 10_1101-2021_01_07_425723 273 186 review review NN 10_1101-2021_01_07_425723 273 187 ) ) -RRB- 10_1101-2021_01_07_425723 273 188 is be VBZ 10_1101-2021_01_07_425723 273 189 the the DT 10_1101-2021_01_07_425723 273 190 author author NN 10_1101-2021_01_07_425723 273 191 / / SYM 10_1101-2021_01_07_425723 273 192 funder funder NN 10_1101-2021_01_07_425723 273 193 , , , 10_1101-2021_01_07_425723 273 194 who who WP 10_1101-2021_01_07_425723 273 195 has have VBZ 10_1101-2021_01_07_425723 273 196 granted grant VBN 10_1101-2021_01_07_425723 273 197 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 273 198 a a DT 10_1101-2021_01_07_425723 273 199 license license NN 10_1101-2021_01_07_425723 273 200 to to TO 10_1101-2021_01_07_425723 273 201 display display VB 10_1101-2021_01_07_425723 273 202 the the DT 10_1101-2021_01_07_425723 273 203 preprint preprint NN 10_1101-2021_01_07_425723 273 204 in in IN 10_1101-2021_01_07_425723 273 205 perpetuity perpetuity NN 10_1101-2021_01_07_425723 273 206 . . . 10_1101-2021_01_07_425723 274 1 It -PRON- PRP 10_1101-2021_01_07_425723 274 2 is be VBZ 10_1101-2021_01_07_425723 274 3 made make VBN 10_1101-2021_01_07_425723 274 4 The the DT 10_1101-2021_01_07_425723 274 5 copyright copyright NN 10_1101-2021_01_07_425723 274 6 holder holder NN 10_1101-2021_01_07_425723 274 7 for for IN 10_1101-2021_01_07_425723 274 8 this this DT 10_1101-2021_01_07_425723 274 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 274 10 version version NN 10_1101-2021_01_07_425723 274 11 posted post VBD 10_1101-2021_01_07_425723 274 12 January January NNP 10_1101-2021_01_07_425723 274 13 8 8 CD 10_1101-2021_01_07_425723 274 14 , , , 10_1101-2021_01_07_425723 274 15 2021 2021 CD 10_1101-2021_01_07_425723 274 16 . . . 10_1101-2021_01_07_425723 274 17 ; ; : 10_1101-2021_01_07_425723 274 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 274 19 : : : 10_1101-2021_01_07_425723 274 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 274 21 preprint preprint NN 10_1101-2021_01_07_425723 274 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 274 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 274 24 25 25 CD 10_1101-2021_01_07_425723 274 25 Table table NN 10_1101-2021_01_07_425723 274 26 3 3 CD 10_1101-2021_01_07_425723 274 27 | | CD 10_1101-2021_01_07_425723 274 28 CRISPR CRISPR NNP 10_1101-2021_01_07_425723 274 29 gRNA grna IN 10_1101-2021_01_07_425723 274 30 target target NN 10_1101-2021_01_07_425723 274 31 sequences sequence NNS 10_1101-2021_01_07_425723 274 32 used use VBN 10_1101-2021_01_07_425723 274 33 in in IN 10_1101-2021_01_07_425723 274 34 this this DT 10_1101-2021_01_07_425723 274 35 study study NN 10_1101-2021_01_07_425723 274 36 . . . 10_1101-2021_01_07_425723 275 1 gRNA gRNA NNP 10_1101-2021_01_07_425723 275 2 target target NN 10_1101-2021_01_07_425723 275 3 sequences sequence NNS 10_1101-2021_01_07_425723 275 4 are be VBP 10_1101-2021_01_07_425723 275 5 shown show VBN 10_1101-2021_01_07_425723 275 6 with with IN 10_1101-2021_01_07_425723 275 7 408 408 CD 10_1101-2021_01_07_425723 275 8 PAM PAM NNP 10_1101-2021_01_07_425723 275 9 sequences sequence NNS 10_1101-2021_01_07_425723 275 10 underlined underline VBD 10_1101-2021_01_07_425723 275 11 . . . 10_1101-2021_01_07_425723 276 1 Position position NN 10_1101-2021_01_07_425723 276 2 in in IN 10_1101-2021_01_07_425723 276 3 ORF ORF NNP 10_1101-2021_01_07_425723 276 4 indicates indicate VBZ 10_1101-2021_01_07_425723 276 5 the the DT 10_1101-2021_01_07_425723 276 6 base base NN 10_1101-2021_01_07_425723 276 7 pair pair NN 10_1101-2021_01_07_425723 276 8 after after IN 10_1101-2021_01_07_425723 276 9 which which WDT 10_1101-2021_01_07_425723 276 10 the the DT 10_1101-2021_01_07_425723 276 11 Cas9-mediated Cas9-mediated NNP 10_1101-2021_01_07_425723 276 12 409 409 CD 10_1101-2021_01_07_425723 276 13 double double JJ 10_1101-2021_01_07_425723 276 14 - - HYPH 10_1101-2021_01_07_425723 276 15 strand strand NN 10_1101-2021_01_07_425723 276 16 break break NN 10_1101-2021_01_07_425723 276 17 is be VBZ 10_1101-2021_01_07_425723 276 18 introduced introduce VBN 10_1101-2021_01_07_425723 276 19 . . . 10_1101-2021_01_07_425723 277 1 AT at IN 10_1101-2021_01_07_425723 277 2 score score NN 10_1101-2021_01_07_425723 277 3 indicates indicate VBZ 10_1101-2021_01_07_425723 277 4 the the DT 10_1101-2021_01_07_425723 277 5 AT AT NNP 10_1101-2021_01_07_425723 277 6 content content NN 10_1101-2021_01_07_425723 277 7 of of IN 10_1101-2021_01_07_425723 277 8 the the DT 10_1101-2021_01_07_425723 277 9 20-bp 20-bp CD 10_1101-2021_01_07_425723 277 10 target target VB 10_1101-2021_01_07_425723 277 11 sequence sequence NN 10_1101-2021_01_07_425723 277 12 and and CC 10_1101-2021_01_07_425723 277 13 410 410 CD 10_1101-2021_01_07_425723 277 14 RNA RNA NNP 10_1101-2021_01_07_425723 277 15 score score NN 10_1101-2021_01_07_425723 277 16 indicates indicate VBZ 10_1101-2021_01_07_425723 277 17 the the DT 10_1101-2021_01_07_425723 277 18 fraction fraction NN 10_1101-2021_01_07_425723 277 19 of of IN 10_1101-2021_01_07_425723 277 20 unpaired unpaired JJ 10_1101-2021_01_07_425723 277 21 nucleotides nucleotide NNS 10_1101-2021_01_07_425723 277 22 of of IN 10_1101-2021_01_07_425723 277 23 the the DT 10_1101-2021_01_07_425723 277 24 20-bp 20-bp CD 10_1101-2021_01_07_425723 277 25 target target NN 10_1101-2021_01_07_425723 277 26 sequence sequence NN 10_1101-2021_01_07_425723 277 27 , , , 10_1101-2021_01_07_425723 277 28 predicted predict VBD 10_1101-2021_01_07_425723 277 29 with with IN 10_1101-2021_01_07_425723 277 30 411 411 CD 10_1101-2021_01_07_425723 277 31 the the DT 10_1101-2021_01_07_425723 277 32 complete complete JJ 10_1101-2021_01_07_425723 277 33 gRNA grna NN 10_1101-2021_01_07_425723 277 34 sequence sequence NN 10_1101-2021_01_07_425723 277 35 using use VBG 10_1101-2021_01_07_425723 277 36 a a DT 10_1101-2021_01_07_425723 277 37 minimum minimum JJ 10_1101-2021_01_07_425723 277 38 free free JJ 10_1101-2021_01_07_425723 277 39 energy energy NN 10_1101-2021_01_07_425723 277 40 prediction prediction NN 10_1101-2021_01_07_425723 277 41 by by IN 10_1101-2021_01_07_425723 277 42 the the DT 10_1101-2021_01_07_425723 277 43 RNAfold rnafold NN 10_1101-2021_01_07_425723 277 44 algorithm62 algorithm62 NN 10_1101-2021_01_07_425723 277 45 . . . 10_1101-2021_01_07_425723 278 1 412 412 CD 10_1101-2021_01_07_425723 278 2 Locus Locus NNP 10_1101-2021_01_07_425723 278 3 Target Target NNP 10_1101-2021_01_07_425723 278 4 sequence sequence NN 10_1101-2021_01_07_425723 278 5 ( ( -LRB- 10_1101-2021_01_07_425723 278 6 5'-3 5'-3 CD 10_1101-2021_01_07_425723 278 7 ' ' POS 10_1101-2021_01_07_425723 278 8 ) ) -RRB- 10_1101-2021_01_07_425723 278 9 Position position NN 10_1101-2021_01_07_425723 278 10 in in IN 10_1101-2021_01_07_425723 278 11 ORF ORF NNP 10_1101-2021_01_07_425723 278 12 ( ( -LRB- 10_1101-2021_01_07_425723 278 13 bp bp NN 10_1101-2021_01_07_425723 278 14 ) ) -RRB- 10_1101-2021_01_07_425723 278 15 AT at IN 10_1101-2021_01_07_425723 278 16 score score NN 10_1101-2021_01_07_425723 278 17 RNA RNA NNP 10_1101-2021_01_07_425723 278 18 score score NN 10_1101-2021_01_07_425723 278 19 AUS1 AUS1 NNP 10_1101-2021_01_07_425723 278 20 CATTATTGTAAATGATTTGGTGG CATTATTGTAAATGATTTGGTGG NNP 10_1101-2021_01_07_425723 278 21 320/4184 320/4184 CD 10_1101-2021_01_07_425723 278 22 0.75 0.75 CD 10_1101-2021_01_07_425723 278 23 1 1 CD 10_1101-2021_01_07_425723 278 24 PDR11 PDR11 NNP 10_1101-2021_01_07_425723 278 25 ATCTTTCATATAAATAACATAGG ATCTTTCATATAAATAACATAGG NNP 10_1101-2021_01_07_425723 278 26 1627/4235 1627/4235 CD 10_1101-2021_01_07_425723 278 27 0.85 0.85 CD 10_1101-2021_01_07_425723 278 28 1 1 CD 10_1101-2021_01_07_425723 278 29 413 413 CD 10_1101-2021_01_07_425723 278 30 Table table NN 10_1101-2021_01_07_425723 278 31 4 4 CD 10_1101-2021_01_07_425723 278 32 | | NNP 10_1101-2021_01_07_425723 278 33 Plasmids Plasmids NNPS 10_1101-2021_01_07_425723 278 34 used use VBN 10_1101-2021_01_07_425723 278 35 in in IN 10_1101-2021_01_07_425723 278 36 this this DT 10_1101-2021_01_07_425723 278 37 study study NN 10_1101-2021_01_07_425723 278 38 . . . 10_1101-2021_01_07_425723 279 1 Restriction restriction NN 10_1101-2021_01_07_425723 279 2 enzyme enzyme NNS 10_1101-2021_01_07_425723 279 3 recognition recognition NN 10_1101-2021_01_07_425723 279 4 sites site NNS 10_1101-2021_01_07_425723 279 5 are be VBP 10_1101-2021_01_07_425723 279 6 indicated indicate VBN 10_1101-2021_01_07_425723 279 7 in in IN 10_1101-2021_01_07_425723 279 8 superscript superscript NN 10_1101-2021_01_07_425723 279 9 . . . 10_1101-2021_01_07_425723 280 1 414 414 CD 10_1101-2021_01_07_425723 280 2 US US NNP 10_1101-2021_01_07_425723 280 3 / / SYM 10_1101-2021_01_07_425723 280 4 DS DS NNP 10_1101-2021_01_07_425723 280 5 represent represent VBP 10_1101-2021_01_07_425723 280 6 upstream upstream JJ 10_1101-2021_01_07_425723 280 7 and and CC 10_1101-2021_01_07_425723 280 8 downstream downstream JJ 10_1101-2021_01_07_425723 280 9 homologous homologous JJ 10_1101-2021_01_07_425723 280 10 recombination recombination NN 10_1101-2021_01_07_425723 280 11 sequences sequence NNS 10_1101-2021_01_07_425723 280 12 used use VBN 10_1101-2021_01_07_425723 280 13 for for IN 10_1101-2021_01_07_425723 280 14 genomic genomic JJ 10_1101-2021_01_07_425723 280 15 415 415 CD 10_1101-2021_01_07_425723 280 16 integration integration NN 10_1101-2021_01_07_425723 280 17 into into IN 10_1101-2021_01_07_425723 280 18 the the DT 10_1101-2021_01_07_425723 280 19 K. K. NNP 10_1101-2021_01_07_425723 280 20 marxianus marxianus NNP 10_1101-2021_01_07_425723 280 21 lac4 lac4 NNP 10_1101-2021_01_07_425723 280 22 locus locus NN 10_1101-2021_01_07_425723 280 23 . . . 10_1101-2021_01_07_425723 281 1 Abbreviations abbreviation NNS 10_1101-2021_01_07_425723 281 2 : : : 10_1101-2021_01_07_425723 281 3 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 281 4 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 281 5 ( ( -LRB- 10_1101-2021_01_07_425723 281 6 Sc Sc NNP 10_1101-2021_01_07_425723 281 7 ) ) -RRB- 10_1101-2021_01_07_425723 281 8 , , , 10_1101-2021_01_07_425723 281 9 416 416 CD 10_1101-2021_01_07_425723 281 10 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 281 11 marxianus marxianus NN 10_1101-2021_01_07_425723 281 12 ( ( -LRB- 10_1101-2021_01_07_425723 281 13 Km Km NNP 10_1101-2021_01_07_425723 281 14 ) ) -RRB- 10_1101-2021_01_07_425723 281 15 , , , 10_1101-2021_01_07_425723 281 16 Tetrahymena Tetrahymena NNP 10_1101-2021_01_07_425723 281 17 thermophila thermophila NN 10_1101-2021_01_07_425723 281 18 ( ( -LRB- 10_1101-2021_01_07_425723 281 19 Tt Tt NNP 10_1101-2021_01_07_425723 281 20 ) ) -RRB- 10_1101-2021_01_07_425723 281 21 . . . 10_1101-2021_01_07_425723 282 1 417 417 CD 10_1101-2021_01_07_425723 282 2 Plasmid Plasmid NNP 10_1101-2021_01_07_425723 282 3 Characteristics Characteristics NNP 10_1101-2021_01_07_425723 282 4 Source Source NNP 10_1101-2021_01_07_425723 282 5 pGGkd015 pggkd015 CD 10_1101-2021_01_07_425723 282 6 ori ori NN 10_1101-2021_01_07_425723 282 7 ampR ampR NNP 10_1101-2021_01_07_425723 282 8 ConLS ConLS NNP 10_1101-2021_01_07_425723 282 9 GFP GFP NNP 10_1101-2021_01_07_425723 282 10 ConR1 ConR1 NNP 10_1101-2021_01_07_425723 282 11 Hassing Hassing NNP 10_1101-2021_01_07_425723 282 12 et et NNP 10_1101-2021_01_07_425723 282 13 al al NNP 10_1101-2021_01_07_425723 282 14 . . NNP 10_1101-2021_01_07_425723 282 15 , , , 10_1101-2021_01_07_425723 282 16 2019 2019 CD 10_1101-2021_01_07_425723 282 17 63 63 CD 10_1101-2021_01_07_425723 282 18 pGGKd068 pGGKd068 NNPS 10_1101-2021_01_07_425723 282 19 ori ori VBP 10_1101-2021_01_07_425723 282 20 kanR kanr CD 10_1101-2021_01_07_425723 282 21 NotIKmlac4US NotIKmlac4US NNP 10_1101-2021_01_07_425723 282 22 BsmBIConRE’BsaIsfGFPBsaI BsmBIConRE’BsaIsfGFPBsaI NNP 10_1101-2021_01_07_425723 282 23 ConLS’BsmBI conls’bsmbi NN 10_1101-2021_01_07_425723 282 24 hygB hygB VBZ 10_1101-2021_01_07_425723 282 25 Kmlac4DSNotI Kmlac4DSNotI VBD 10_1101-2021_01_07_425723 282 26 This this DT 10_1101-2021_01_07_425723 282 27 study study NN 10_1101-2021_01_07_425723 282 28 pP2 pp2 NN 10_1101-2021_01_07_425723 282 29 ori ori NN 10_1101-2021_01_07_425723 282 30 camR camR VBZ 10_1101-2021_01_07_425723 282 31 KmPDC1p KmPDC1p NNP 10_1101-2021_01_07_425723 282 32 Rajkumar Rajkumar NNP 10_1101-2021_01_07_425723 282 33 et et FW 10_1101-2021_01_07_425723 282 34 al al NNP 10_1101-2021_01_07_425723 282 35 . . NNP 10_1101-2021_01_07_425723 282 36 , , , 10_1101-2021_01_07_425723 282 37 2019 2019 CD 10_1101-2021_01_07_425723 282 38 47 47 CD 10_1101-2021_01_07_425723 282 39 pROS11 pros11 CD 10_1101-2021_01_07_425723 282 40 ori ori NN 10_1101-2021_01_07_425723 282 41 ampR ampR NNP 10_1101-2021_01_07_425723 282 42 2μm 2μm JJ 10_1101-2021_01_07_425723 282 43 amdSYM amdSYM NNP 10_1101-2021_01_07_425723 282 44 pSNR52-gRNACAN1 psnr52-grnacan1 NN 10_1101-2021_01_07_425723 282 45 pRSNR52-gRNAADE2 pRSNR52-gRNAADE2 NNP 10_1101-2021_01_07_425723 282 46 Mans Mans NNPS 10_1101-2021_01_07_425723 282 47 et et NNP 10_1101-2021_01_07_425723 282 48 al al NNP 10_1101-2021_01_07_425723 282 49 . . NNP 10_1101-2021_01_07_425723 282 50 , , , 10_1101-2021_01_07_425723 282 51 2015 2015 CD 10_1101-2021_01_07_425723 282 52 61 61 CD 10_1101-2021_01_07_425723 282 53 pUD696 pUD696 NNP 10_1101-2021_01_07_425723 282 54 ori ori VBP 10_1101-2021_01_07_425723 282 55 kanR kanr CD 10_1101-2021_01_07_425723 282 56 TtSTC1 ttstc1 CD 10_1101-2021_01_07_425723 282 57 Wiersma Wiersma NNP 10_1101-2021_01_07_425723 282 58 et et NNP 10_1101-2021_01_07_425723 282 59 al al NNP 10_1101-2021_01_07_425723 282 60 . . NNP 10_1101-2021_01_07_425723 282 61 , , , 10_1101-2021_01_07_425723 282 62 2020 2020 CD 10_1101-2021_01_07_425723 282 63 46 46 CD 10_1101-2021_01_07_425723 282 64 pUDE659 pUDE659 NNPS 10_1101-2021_01_07_425723 282 65 ori ori VB 10_1101-2021_01_07_425723 282 66 ampR ampR NNP 10_1101-2021_01_07_425723 282 67 2μm 2μm JJ 10_1101-2021_01_07_425723 282 68 amdSYM amdSYM NNP 10_1101-2021_01_07_425723 282 69 pSNR52-gRNAAUS1 psnr52-grnaaus1 NN 10_1101-2021_01_07_425723 282 70 pRSNR52-gRNAAUS1 pRSNR52-gRNAAUS1 NNS 10_1101-2021_01_07_425723 282 71 This this DT 10_1101-2021_01_07_425723 282 72 study study NN 10_1101-2021_01_07_425723 282 73 pUDE663 pUDE663 NNP 10_1101-2021_01_07_425723 282 74 ori ori VBP 10_1101-2021_01_07_425723 282 75 ampR ampr JJ 10_1101-2021_01_07_425723 282 76 2μm 2μm JJ 10_1101-2021_01_07_425723 282 77 amdSYM amdSYM NNP 10_1101-2021_01_07_425723 282 78 pSNR52-gRNAPDR11 pSNR52-gRNAPDR11 NNP 10_1101-2021_01_07_425723 282 79 pRSNR52-gRNAPDR11 pRSNR52-gRNAPDR11 NNP 10_1101-2021_01_07_425723 282 80 This this DT 10_1101-2021_01_07_425723 282 81 study study NN 10_1101-2021_01_07_425723 282 82 pUDE909 pUDE909 NNS 10_1101-2021_01_07_425723 282 83 ori ori VBP 10_1101-2021_01_07_425723 282 84 ampR ampR NNP 10_1101-2021_01_07_425723 282 85 KmPDC1p KmPDC1p NNP 10_1101-2021_01_07_425723 282 86 - - HYPH 10_1101-2021_01_07_425723 282 87 TtSTC1-ScADH1 TtSTC1-ScADH1 NNP 10_1101-2021_01_07_425723 282 88 t t NN 10_1101-2021_01_07_425723 282 89 This this DT 10_1101-2021_01_07_425723 282 90 study study NN 10_1101-2021_01_07_425723 282 91 pUDI246 pUDI246 NNP 10_1101-2021_01_07_425723 282 92 ori ori VBP 10_1101-2021_01_07_425723 282 93 kanR kanr CD 10_1101-2021_01_07_425723 282 94 NotIKmlac4US NotIKmlac4US NNP 10_1101-2021_01_07_425723 282 95 KmPDC1p KmPDC1p NNP 10_1101-2021_01_07_425723 282 96 - - : 10_1101-2021_01_07_425723 282 97 TtSTC1-ScADH1 TtSTC1-ScADH1 NNP 10_1101-2021_01_07_425723 282 98 t t NN 10_1101-2021_01_07_425723 282 99 hygB hygB NNS 10_1101-2021_01_07_425723 282 100 Kmlac4DSNotI Kmlac4DSNotI VBD 10_1101-2021_01_07_425723 282 101 This this DT 10_1101-2021_01_07_425723 282 102 study study NN 10_1101-2021_01_07_425723 282 103 pYTK008 pytk008 NN 10_1101-2021_01_07_425723 282 104 ori ori NN 10_1101-2021_01_07_425723 282 105 camR camR NNP 10_1101-2021_01_07_425723 282 106 ConLS ConLS NNP 10_1101-2021_01_07_425723 282 107 ’ ' '' 10_1101-2021_01_07_425723 282 108 Lee Lee NNP 10_1101-2021_01_07_425723 282 109 et et NNP 10_1101-2021_01_07_425723 282 110 al al NNP 10_1101-2021_01_07_425723 282 111 . . NNP 10_1101-2021_01_07_425723 282 112 , , , 10_1101-2021_01_07_425723 282 113 2015 2015 CD 10_1101-2021_01_07_425723 282 114 60 60 CD 10_1101-2021_01_07_425723 282 115 pYTK047 pYTK047 NNS 10_1101-2021_01_07_425723 282 116 ori ori RB 10_1101-2021_01_07_425723 282 117 camR camr JJ 10_1101-2021_01_07_425723 282 118 GFP GFP NNP 10_1101-2021_01_07_425723 282 119 dropout dropout NN 10_1101-2021_01_07_425723 282 120 Lee Lee NNP 10_1101-2021_01_07_425723 282 121 et et FW 10_1101-2021_01_07_425723 282 122 al al NNP 10_1101-2021_01_07_425723 282 123 . . NNP 10_1101-2021_01_07_425723 282 124 , , , 10_1101-2021_01_07_425723 282 125 2015 2015 CD 10_1101-2021_01_07_425723 282 126 60 60 CD 10_1101-2021_01_07_425723 282 127 pYTK053 pYTK053 NNP 10_1101-2021_01_07_425723 282 128 ori ori NN 10_1101-2021_01_07_425723 282 129 camR camR NNP 10_1101-2021_01_07_425723 282 130 ScADH1 ScADH1 NNP 10_1101-2021_01_07_425723 282 131 t t NNP 10_1101-2021_01_07_425723 282 132 Lee Lee NNP 10_1101-2021_01_07_425723 282 133 et et FW 10_1101-2021_01_07_425723 282 134 al al NNP 10_1101-2021_01_07_425723 282 135 . . NNP 10_1101-2021_01_07_425723 282 136 , , , 10_1101-2021_01_07_425723 282 137 2015 2015 CD 10_1101-2021_01_07_425723 282 138 60 60 CD 10_1101-2021_01_07_425723 282 139 pYTK073 pYTK073 NNP 10_1101-2021_01_07_425723 282 140 ori ori NN 10_1101-2021_01_07_425723 282 141 camR camr NN 10_1101-2021_01_07_425723 282 142 ConRE ConRE NNP 10_1101-2021_01_07_425723 282 143 ' ' '' 10_1101-2021_01_07_425723 282 144 Lee Lee NNP 10_1101-2021_01_07_425723 282 145 et et NNP 10_1101-2021_01_07_425723 282 146 al al NNP 10_1101-2021_01_07_425723 282 147 . . NNP 10_1101-2021_01_07_425723 282 148 , , , 10_1101-2021_01_07_425723 282 149 2015 2015 CD 10_1101-2021_01_07_425723 282 150 60 60 CD 10_1101-2021_01_07_425723 282 151 pYTK079 pYTK079 NNP 10_1101-2021_01_07_425723 282 152 ori ori NN 10_1101-2021_01_07_425723 282 153 camR camr NN 10_1101-2021_01_07_425723 282 154 hygB hygb ADD 10_1101-2021_01_07_425723 282 155 Lee Lee NNP 10_1101-2021_01_07_425723 282 156 et et NNP 10_1101-2021_01_07_425723 282 157 al al NNP 10_1101-2021_01_07_425723 282 158 . . NNP 10_1101-2021_01_07_425723 282 159 , , , 10_1101-2021_01_07_425723 282 160 2015 2015 CD 10_1101-2021_01_07_425723 282 161 60 60 CD 10_1101-2021_01_07_425723 282 162 418 418 CD 10_1101-2021_01_07_425723 282 163 .CC .CC : 10_1101-2021_01_07_425723 282 164 - - HYPH 10_1101-2021_01_07_425723 282 165 BY by IN 10_1101-2021_01_07_425723 282 166 - - HYPH 10_1101-2021_01_07_425723 282 167 NC NC NNP 10_1101-2021_01_07_425723 282 168 - - HYPH 10_1101-2021_01_07_425723 282 169 ND ND NNP 10_1101-2021_01_07_425723 282 170 4.0 4.0 CD 10_1101-2021_01_07_425723 282 171 International International NNP 10_1101-2021_01_07_425723 282 172 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 282 173 under under IN 10_1101-2021_01_07_425723 282 174 a a DT 10_1101-2021_01_07_425723 282 175 ( ( -LRB- 10_1101-2021_01_07_425723 282 176 which which WDT 10_1101-2021_01_07_425723 282 177 was be VBD 10_1101-2021_01_07_425723 282 178 not not RB 10_1101-2021_01_07_425723 282 179 certified certify VBN 10_1101-2021_01_07_425723 282 180 by by IN 10_1101-2021_01_07_425723 282 181 peer peer NN 10_1101-2021_01_07_425723 282 182 review review NN 10_1101-2021_01_07_425723 282 183 ) ) -RRB- 10_1101-2021_01_07_425723 282 184 is be VBZ 10_1101-2021_01_07_425723 282 185 the the DT 10_1101-2021_01_07_425723 282 186 author author NN 10_1101-2021_01_07_425723 282 187 / / SYM 10_1101-2021_01_07_425723 282 188 funder funder NN 10_1101-2021_01_07_425723 282 189 , , , 10_1101-2021_01_07_425723 282 190 who who WP 10_1101-2021_01_07_425723 282 191 has have VBZ 10_1101-2021_01_07_425723 282 192 granted grant VBN 10_1101-2021_01_07_425723 282 193 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 282 194 a a DT 10_1101-2021_01_07_425723 282 195 license license NN 10_1101-2021_01_07_425723 282 196 to to TO 10_1101-2021_01_07_425723 282 197 display display VB 10_1101-2021_01_07_425723 282 198 the the DT 10_1101-2021_01_07_425723 282 199 preprint preprint NN 10_1101-2021_01_07_425723 282 200 in in IN 10_1101-2021_01_07_425723 282 201 perpetuity perpetuity NN 10_1101-2021_01_07_425723 282 202 . . . 10_1101-2021_01_07_425723 283 1 It -PRON- PRP 10_1101-2021_01_07_425723 283 2 is be VBZ 10_1101-2021_01_07_425723 283 3 made make VBN 10_1101-2021_01_07_425723 283 4 The the DT 10_1101-2021_01_07_425723 283 5 copyright copyright NN 10_1101-2021_01_07_425723 283 6 holder holder NN 10_1101-2021_01_07_425723 283 7 for for IN 10_1101-2021_01_07_425723 283 8 this this DT 10_1101-2021_01_07_425723 283 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 283 10 version version NN 10_1101-2021_01_07_425723 283 11 posted post VBD 10_1101-2021_01_07_425723 283 12 January January NNP 10_1101-2021_01_07_425723 283 13 8 8 CD 10_1101-2021_01_07_425723 283 14 , , , 10_1101-2021_01_07_425723 283 15 2021 2021 CD 10_1101-2021_01_07_425723 283 16 . . . 10_1101-2021_01_07_425723 283 17 ; ; : 10_1101-2021_01_07_425723 283 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 283 19 : : : 10_1101-2021_01_07_425723 283 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 283 21 preprint preprint NN 10_1101-2021_01_07_425723 283 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 283 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CC 10_1101-2021_01_07_425723 283 24 26 26 CD 10_1101-2021_01_07_425723 283 25 Table table NN 10_1101-2021_01_07_425723 283 26 5 5 CD 10_1101-2021_01_07_425723 283 27 | | CD 10_1101-2021_01_07_425723 283 28 Oligonucleotide oligonucleotide JJ 10_1101-2021_01_07_425723 283 29 primers primer NNS 10_1101-2021_01_07_425723 283 30 used use VBN 10_1101-2021_01_07_425723 283 31 in in IN 10_1101-2021_01_07_425723 283 32 this this DT 10_1101-2021_01_07_425723 283 33 study study NN 10_1101-2021_01_07_425723 283 34 . . . 10_1101-2021_01_07_425723 284 1 419 419 CD 10_1101-2021_01_07_425723 284 2 Primer Primer NNP 10_1101-2021_01_07_425723 284 3 Sequence Sequence NNP 10_1101-2021_01_07_425723 284 4 ( ( -LRB- 10_1101-2021_01_07_425723 284 5 5'->3 5'->3 CD 10_1101-2021_01_07_425723 284 6 ' ' POS 10_1101-2021_01_07_425723 284 7 ) ) -RRB- 10_1101-2021_01_07_425723 284 8 11228 11228 CD 10_1101-2021_01_07_425723 284 9 TGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATCCATTATTGTAAATGATTTGGGTTTTA TGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATCCATTATTGTAAATGATTTGGGTTTTA NNP 10_1101-2021_01_07_425723 284 10 GAGCTAGAAATAGCAAGTTAAAATAAG GAGCTAGAAATAGCAAGTTAAAATAAG NNP 10_1101-2021_01_07_425723 284 11 11232 11232 CD 10_1101-2021_01_07_425723 284 12 TGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATCATCTTTCATATAAATAACATGTTTTA TGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATCATCTTTCATATAAATAACATGTTTTA NNP 10_1101-2021_01_07_425723 284 13 GAGCTAGAAATAGCAAGTTAAAATAAG GAGCTAGAAATAGCAAGTTAAAATAAG NNP 10_1101-2021_01_07_425723 284 14 11233 11233 CD 10_1101-2021_01_07_425723 284 15 TAGTAAAGACTGCTGTAATTCATCTCTCAGTCCTTGCAGTCTGCTTTTTCTGGAATTAATTACCATTTTTAAATAT TAGTAAAGACTGCTGTAATTCATCTCTCAGTCCTTGCAGTCTGCTTTTTCTGGAATTAATTACCATTTTTAAATAT NNP 10_1101-2021_01_07_425723 284 16 ATTTCTACTTTCTACTTAATAGCAATTTTAATTAATCTAATTAT atttctactttctacttaatagcaattttaattaatctaattat NN 10_1101-2021_01_07_425723 284 17 11234 11234 CD 10_1101-2021_01_07_425723 284 18 ATAATTAGATTAATTAAAATTGCTATTAAGTAGAAAGTAGAAATATATTTAAAAATGGTAATTAATTCCAGAAAAA ATAATTAGATTAATTAAAATTGCTATTAAGTAGAAAGTAGAAATATATTTAAAAATGGTAATTAATTCCAGAAAAA NNP 10_1101-2021_01_07_425723 284 19 GCAGACTGCAAGGACTGAGAGATGAATTACAGCAGTCTTTACTA GCAGACTGCAAGGACTGAGAGATGAATTACAGCAGTCTTTACTA NNP 10_1101-2021_01_07_425723 284 20 11241 11241 CD 10_1101-2021_01_07_425723 284 21 TAGCAAAAAAATTCACAACTAAACACGATAGAGTAAAATTAGAGAAGCAACGCCTCGCGGTCAGTGAATAGCGTTC tagcaaaaaaattcacaactaaacacgatagagtaaaattagagaagcaacgcctcgcggtcagtgaatagcgttc NN 10_1101-2021_01_07_425723 284 22 CGTTAGAAAACATTCAAAATTACCTAATACTATTCAACAGTTCT CGTTAGAAAACATTCAAAATTACCTAATACTATTCAACAGTTCT VBD 10_1101-2021_01_07_425723 284 23 11242 11242 CD 10_1101-2021_01_07_425723 284 24 AGAACTGTTGAATAGTATTAGGTAATTTTGAATGTTTTCTAACGGAACGCTATTCACTGACCGCGAGGCGTTGCTT AGAACTGTTGAATAGTATTAGGTAATTTTGAATGTTTTCTAACGGAACGCTATTCACTGACCGCGAGGCGTTGCTT NNP 10_1101-2021_01_07_425723 284 25 CTCTAATTTTACTCTATCGTGTTTAGTTGTGAATTTTTTTGCTA CTCTAATTTTACTCTATCGTGTTTAGTTGTGAATTTTTTTGCTA NNP 10_1101-2021_01_07_425723 284 26 11243 11243 CD 10_1101-2021_01_07_425723 284 27 TGTCACTACAGCCACAGCAG tgtcactacagccacagcag NN 10_1101-2021_01_07_425723 284 28 11244 11244 CD 10_1101-2021_01_07_425723 284 29 TTGGTAAGGCGCCACACTAG TTGGTAAGGCGCCACACTAG VBD 10_1101-2021_01_07_425723 284 30 11251 11251 CD 10_1101-2021_01_07_425723 284 31 AGAGAAGCGCCACATAGACG AGAGAAGCGCCACATAGACG NNP 10_1101-2021_01_07_425723 284 32 11252 11252 CD 10_1101-2021_01_07_425723 284 33 TGCATATGCTACGGGTGACG tgcatatgctacgggtgacg NN 10_1101-2021_01_07_425723 284 34 11897 11897 CD 10_1101-2021_01_07_425723 284 35 CACCCAAGTATGGTGGGTAG CACCCAAGTATGGTGGGTAG VBD 10_1101-2021_01_07_425723 284 36 14148 14148 CD 10_1101-2021_01_07_425723 284 37 AAGCATCGTCTCATCGGTCTCATATGTCAATTTCAAAGTACTTCACTCCCGTTGCTGAC AAGCATCGTCTCATCGGTCTCATATGTCAATTTCAAAGTACTTCACTCCCGTTGCTGAC NNP 10_1101-2021_01_07_425723 284 38 14149 14149 CD 10_1101-2021_01_07_425723 284 39 TTATGCCGTCTCAGGTCTCAGGATTTAGTTCTGTACAGGCTTCTTC ttatgccgtctcaggtctcaggatttagttctgtacaggcttcttc NN 10_1101-2021_01_07_425723 284 40 14150 14150 CD 10_1101-2021_01_07_425723 284 41 TTATGCCGTCTCAGGTCTCAAGAATTAGTTCTGTACAGGCTTCTTC ttatgccgtctcaggtctcaagaattagttctgtacaggcttcttc NN 10_1101-2021_01_07_425723 284 42 14151 14151 CD 10_1101-2021_01_07_425723 284 43 AAGCATCGTCTCATCGGTCTCATATGTCTTTCACTAAAATCGCTGCCTTATTAG aagcatcgtctcatcggtctcatatgtctttcactaaaatcgctgccttattag JJ 10_1101-2021_01_07_425723 284 44 14152 14152 CD 10_1101-2021_01_07_425723 284 45 TTATGCCGTCTCAGGTCTCAGGATATCATAAGAGCATAGCAGCGGCACCGGCAATAG TTATGCCGTCTCAGGTCTCAGGATATCATAAGAGCATAGCAGCGGCACCGGCAATAG NNP 10_1101-2021_01_07_425723 284 46 14197 14197 CD 10_1101-2021_01_07_425723 284 47 AAGCATCGTCTCATCGGTCTCACAATGAAAGTGATTGAAGAACCCTCAAAC AAGCATCGTCTCATCGGTCTCACAATGAAAGTGATTGAAGAACCCTCAAAC NNP 10_1101-2021_01_07_425723 284 48 14198 14198 CD 10_1101-2021_01_07_425723 284 49 TTATGCCGTCTCAGGTCTCAAGGGTTAAGCAATTGGATCCTACC TTATGCCGTCTCAGGTCTCAAGGGTTAAGCAATTGGATCCTACC NNP 10_1101-2021_01_07_425723 284 50 14199 14199 CD 10_1101-2021_01_07_425723 284 51 AAGCATCGTCTCATCGGTCTCAGAGTTGCTTAATTAGCTTGTACATGGCTTTG AAGCATCGTCTCATCGGTCTCAGAGTTGCTTAATTAGCTTGTACATGGCTTTG NNP 10_1101-2021_01_07_425723 284 52 14200 14200 CD 10_1101-2021_01_07_425723 284 53 TTATGCCGTCTCAGGTCTCATCGGGAAGGCCCATATTGAAGACG ttatgccgtctcaggtctcatcgggaaggcccatattgaagacg NN 10_1101-2021_01_07_425723 284 54 14339 14339 CD 10_1101-2021_01_07_425723 284 55 CCCAAATCATTTACAATAATGGATCATTTATC cccaaatcatttacaataatggatcatttatc NN 10_1101-2021_01_07_425723 284 56 14340 14340 CD 10_1101-2021_01_07_425723 284 57 CATGTTATTTATATGAAAGATGATCATTTATC catgttatttatatgaaagatgatcatttatc NN 10_1101-2021_01_07_425723 284 58 16366 16366 CD 10_1101-2021_01_07_425723 284 59 GTCCCTAGGTTCGTCATT GTCCCTAGGTTCGTCATT NNP 10_1101-2021_01_07_425723 284 60 16367 16367 CD 10_1101-2021_01_07_425723 284 61 CAAGATCAATGGTGGCTCTC CAAGATCAATGGTGGCTCTC NNP 10_1101-2021_01_07_425723 284 62 420 420 CD 10_1101-2021_01_07_425723 284 63 Strain strain NN 10_1101-2021_01_07_425723 284 64 construction construction NN 10_1101-2021_01_07_425723 284 65 421 421 CD 10_1101-2021_01_07_425723 284 66 The the DT 10_1101-2021_01_07_425723 284 67 lithium lithium NN 10_1101-2021_01_07_425723 284 68 - - HYPH 10_1101-2021_01_07_425723 284 69 acetate acetate JJ 10_1101-2021_01_07_425723 284 70 / / SYM 10_1101-2021_01_07_425723 284 71 polyethylene polyethylene NN 10_1101-2021_01_07_425723 284 72 - - HYPH 10_1101-2021_01_07_425723 284 73 glycol glycol JJ 10_1101-2021_01_07_425723 284 74 method method NN 10_1101-2021_01_07_425723 284 75 was be VBD 10_1101-2021_01_07_425723 284 76 used use VBN 10_1101-2021_01_07_425723 284 77 for for IN 10_1101-2021_01_07_425723 284 78 yeast yeast NN 10_1101-2021_01_07_425723 284 79 transformation64 transformation64 NN 10_1101-2021_01_07_425723 284 80 . . . 10_1101-2021_01_07_425723 285 1 Homologous homologous JJ 10_1101-2021_01_07_425723 285 2 422 422 CD 10_1101-2021_01_07_425723 285 3 repair repair NN 10_1101-2021_01_07_425723 285 4 ( ( -LRB- 10_1101-2021_01_07_425723 285 5 HR hr NN 10_1101-2021_01_07_425723 285 6 ) ) -RRB- 10_1101-2021_01_07_425723 285 7 DNA dna NN 10_1101-2021_01_07_425723 285 8 fragments fragment NNS 10_1101-2021_01_07_425723 285 9 for for IN 10_1101-2021_01_07_425723 285 10 markerless markerless JJ 10_1101-2021_01_07_425723 285 11 CRISPR CRISPR NNP 10_1101-2021_01_07_425723 285 12 - - HYPH 10_1101-2021_01_07_425723 285 13 Cas9-mediated cas9-mediate VBN 10_1101-2021_01_07_425723 285 14 gene gene NN 10_1101-2021_01_07_425723 285 15 deletions deletion NNS 10_1101-2021_01_07_425723 285 16 in in IN 10_1101-2021_01_07_425723 285 17 S. S. NNP 10_1101-2021_01_07_425723 285 18 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 285 19 were be VBD 10_1101-2021_01_07_425723 285 20 423 423 CD 10_1101-2021_01_07_425723 285 21 constructed construct VBN 10_1101-2021_01_07_425723 285 22 by by IN 10_1101-2021_01_07_425723 285 23 annealing anneal VBG 10_1101-2021_01_07_425723 285 24 two two CD 10_1101-2021_01_07_425723 285 25 120 120 CD 10_1101-2021_01_07_425723 285 26 bp bp NN 10_1101-2021_01_07_425723 285 27 primers primer NNS 10_1101-2021_01_07_425723 285 28 , , , 10_1101-2021_01_07_425723 285 29 using use VBG 10_1101-2021_01_07_425723 285 30 primer primer NNP 10_1101-2021_01_07_425723 285 31 pairs pairs NNP 10_1101-2021_01_07_425723 285 32 11241/11242 11241/11242 CD 10_1101-2021_01_07_425723 285 33 and and CC 10_1101-2021_01_07_425723 285 34 11233/11234 11233/11234 CD 10_1101-2021_01_07_425723 285 35 for for IN 10_1101-2021_01_07_425723 285 36 424 424 CD 10_1101-2021_01_07_425723 285 37 deletion deletion NN 10_1101-2021_01_07_425723 285 38 of of IN 10_1101-2021_01_07_425723 285 39 PDR11 PDR11 NNP 10_1101-2021_01_07_425723 285 40 and and CC 10_1101-2021_01_07_425723 285 41 AUS1 AUS1 NNP 10_1101-2021_01_07_425723 285 42 , , , 10_1101-2021_01_07_425723 285 43 respectively respectively RB 10_1101-2021_01_07_425723 285 44 . . . 10_1101-2021_01_07_425723 286 1 After after IN 10_1101-2021_01_07_425723 286 2 transformation transformation NN 10_1101-2021_01_07_425723 286 3 of of IN 10_1101-2021_01_07_425723 286 4 S. S. NNP 10_1101-2021_01_07_425723 286 5 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 286 6 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 286 7 with with IN 10_1101-2021_01_07_425723 286 8 gRNA grna NN 10_1101-2021_01_07_425723 286 9 425 425 CD 10_1101-2021_01_07_425723 286 10 plasmids plasmid NNS 10_1101-2021_01_07_425723 286 11 pUDE659 pUDE659 NNPS 10_1101-2021_01_07_425723 286 12 and and CC 10_1101-2021_01_07_425723 286 13 pUDE663 pUDE663 NNP 10_1101-2021_01_07_425723 286 14 and and CC 10_1101-2021_01_07_425723 286 15 double double JJ 10_1101-2021_01_07_425723 286 16 - - HYPH 10_1101-2021_01_07_425723 286 17 stranded strand VBN 10_1101-2021_01_07_425723 286 18 repair repair NN 10_1101-2021_01_07_425723 286 19 fragments fragment NNS 10_1101-2021_01_07_425723 286 20 , , , 10_1101-2021_01_07_425723 286 21 transformants transformant NNS 10_1101-2021_01_07_425723 286 22 were be VBD 10_1101-2021_01_07_425723 286 23 selected select VBN 10_1101-2021_01_07_425723 286 24 426 426 CD 10_1101-2021_01_07_425723 286 25 on on IN 10_1101-2021_01_07_425723 286 26 synthetic synthetic JJ 10_1101-2021_01_07_425723 286 27 medium medium NN 10_1101-2021_01_07_425723 286 28 with with IN 10_1101-2021_01_07_425723 286 29 acetamide acetamide NN 10_1101-2021_01_07_425723 286 30 as as IN 10_1101-2021_01_07_425723 286 31 sole sole JJ 10_1101-2021_01_07_425723 286 32 nitrogen nitrogen NN 10_1101-2021_01_07_425723 286 33 source65 source65 VBD 10_1101-2021_01_07_425723 286 34 . . . 10_1101-2021_01_07_425723 287 1 Deletion deletion NN 10_1101-2021_01_07_425723 287 2 of of IN 10_1101-2021_01_07_425723 287 3 AUS1 AUS1 NNP 10_1101-2021_01_07_425723 287 4 and and CC 10_1101-2021_01_07_425723 287 5 PDR11 PDR11 NNP 10_1101-2021_01_07_425723 287 6 was be VBD 10_1101-2021_01_07_425723 287 7 427 427 CD 10_1101-2021_01_07_425723 287 8 .CC .CC : 10_1101-2021_01_07_425723 287 9 - - HYPH 10_1101-2021_01_07_425723 287 10 BY by IN 10_1101-2021_01_07_425723 287 11 - - HYPH 10_1101-2021_01_07_425723 287 12 NC NC NNP 10_1101-2021_01_07_425723 287 13 - - HYPH 10_1101-2021_01_07_425723 287 14 ND ND NNP 10_1101-2021_01_07_425723 287 15 4.0 4.0 CD 10_1101-2021_01_07_425723 287 16 International International NNP 10_1101-2021_01_07_425723 287 17 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 287 18 under under IN 10_1101-2021_01_07_425723 287 19 a a DT 10_1101-2021_01_07_425723 287 20 ( ( -LRB- 10_1101-2021_01_07_425723 287 21 which which WDT 10_1101-2021_01_07_425723 287 22 was be VBD 10_1101-2021_01_07_425723 287 23 not not RB 10_1101-2021_01_07_425723 287 24 certified certify VBN 10_1101-2021_01_07_425723 287 25 by by IN 10_1101-2021_01_07_425723 287 26 peer peer NN 10_1101-2021_01_07_425723 287 27 review review NN 10_1101-2021_01_07_425723 287 28 ) ) -RRB- 10_1101-2021_01_07_425723 287 29 is be VBZ 10_1101-2021_01_07_425723 287 30 the the DT 10_1101-2021_01_07_425723 287 31 author author NN 10_1101-2021_01_07_425723 287 32 / / SYM 10_1101-2021_01_07_425723 287 33 funder funder NN 10_1101-2021_01_07_425723 287 34 , , , 10_1101-2021_01_07_425723 287 35 who who WP 10_1101-2021_01_07_425723 287 36 has have VBZ 10_1101-2021_01_07_425723 287 37 granted grant VBN 10_1101-2021_01_07_425723 287 38 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 287 39 a a DT 10_1101-2021_01_07_425723 287 40 license license NN 10_1101-2021_01_07_425723 287 41 to to TO 10_1101-2021_01_07_425723 287 42 display display VB 10_1101-2021_01_07_425723 287 43 the the DT 10_1101-2021_01_07_425723 287 44 preprint preprint NN 10_1101-2021_01_07_425723 287 45 in in IN 10_1101-2021_01_07_425723 287 46 perpetuity perpetuity NN 10_1101-2021_01_07_425723 287 47 . . . 10_1101-2021_01_07_425723 288 1 It -PRON- PRP 10_1101-2021_01_07_425723 288 2 is be VBZ 10_1101-2021_01_07_425723 288 3 made make VBN 10_1101-2021_01_07_425723 288 4 The the DT 10_1101-2021_01_07_425723 288 5 copyright copyright NN 10_1101-2021_01_07_425723 288 6 holder holder NN 10_1101-2021_01_07_425723 288 7 for for IN 10_1101-2021_01_07_425723 288 8 this this DT 10_1101-2021_01_07_425723 288 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 288 10 version version NN 10_1101-2021_01_07_425723 288 11 posted post VBD 10_1101-2021_01_07_425723 288 12 January January NNP 10_1101-2021_01_07_425723 288 13 8 8 CD 10_1101-2021_01_07_425723 288 14 , , , 10_1101-2021_01_07_425723 288 15 2021 2021 CD 10_1101-2021_01_07_425723 288 16 . . . 10_1101-2021_01_07_425723 288 17 ; ; : 10_1101-2021_01_07_425723 288 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 288 19 : : : 10_1101-2021_01_07_425723 288 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 288 21 preprint preprint NN 10_1101-2021_01_07_425723 288 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 288 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 288 24 27 27 CD 10_1101-2021_01_07_425723 288 25 confirmed confirm VBN 10_1101-2021_01_07_425723 288 26 by by IN 10_1101-2021_01_07_425723 288 27 PCR PCR NNP 10_1101-2021_01_07_425723 288 28 amplification amplification NN 10_1101-2021_01_07_425723 288 29 with with IN 10_1101-2021_01_07_425723 288 30 primer primer NNP 10_1101-2021_01_07_425723 288 31 pairs pairs NNP 10_1101-2021_01_07_425723 288 32 11243/11244 11243/11244 CD 10_1101-2021_01_07_425723 288 33 and and CC 10_1101-2021_01_07_425723 288 34 11251/11252 11251/11252 CD 10_1101-2021_01_07_425723 288 35 , , , 10_1101-2021_01_07_425723 288 36 respectively respectively RB 10_1101-2021_01_07_425723 288 37 . . . 10_1101-2021_01_07_425723 289 1 Loss loss NN 10_1101-2021_01_07_425723 289 2 of of IN 10_1101-2021_01_07_425723 289 3 428 428 CD 10_1101-2021_01_07_425723 289 4 gRNA grna NN 10_1101-2021_01_07_425723 289 5 plasmids plasmid NNS 10_1101-2021_01_07_425723 289 6 was be VBD 10_1101-2021_01_07_425723 289 7 induced induce VBN 10_1101-2021_01_07_425723 289 8 by by IN 10_1101-2021_01_07_425723 289 9 cultivation cultivation NN 10_1101-2021_01_07_425723 289 10 of of IN 10_1101-2021_01_07_425723 289 11 single single JJ 10_1101-2021_01_07_425723 289 12 - - HYPH 10_1101-2021_01_07_425723 289 13 colony colony NN 10_1101-2021_01_07_425723 289 14 isolates isolate NNS 10_1101-2021_01_07_425723 289 15 on on IN 10_1101-2021_01_07_425723 289 16 YPD YPD NNP 10_1101-2021_01_07_425723 289 17 , , , 10_1101-2021_01_07_425723 289 18 after after IN 10_1101-2021_01_07_425723 289 19 which which WDT 10_1101-2021_01_07_425723 289 20 plasmid plasmid NN 10_1101-2021_01_07_425723 289 21 loss loss NN 10_1101-2021_01_07_425723 289 22 was be VBD 10_1101-2021_01_07_425723 289 23 429 429 CD 10_1101-2021_01_07_425723 289 24 assessed assess VBN 10_1101-2021_01_07_425723 289 25 by by IN 10_1101-2021_01_07_425723 289 26 absence absence NN 10_1101-2021_01_07_425723 289 27 of of IN 10_1101-2021_01_07_425723 289 28 growth growth NN 10_1101-2021_01_07_425723 289 29 of of IN 10_1101-2021_01_07_425723 289 30 single single JJ 10_1101-2021_01_07_425723 289 31 - - HYPH 10_1101-2021_01_07_425723 289 32 cell cell NN 10_1101-2021_01_07_425723 289 33 isolates isolate NNS 10_1101-2021_01_07_425723 289 34 on on IN 10_1101-2021_01_07_425723 289 35 synthetic synthetic JJ 10_1101-2021_01_07_425723 289 36 medium medium NN 10_1101-2021_01_07_425723 289 37 with with IN 10_1101-2021_01_07_425723 289 38 acetamide acetamide NN 10_1101-2021_01_07_425723 289 39 as as IN 10_1101-2021_01_07_425723 289 40 nitrogen nitrogen NN 10_1101-2021_01_07_425723 289 41 430 430 CD 10_1101-2021_01_07_425723 289 42 source source NN 10_1101-2021_01_07_425723 289 43 . . . 10_1101-2021_01_07_425723 290 1 An an DT 10_1101-2021_01_07_425723 290 2 aus1Δ aus1Δ NNP 10_1101-2021_01_07_425723 290 3 pdr11Δ pdr11Δ NNP 10_1101-2021_01_07_425723 290 4 double double JJ 10_1101-2021_01_07_425723 290 5 - - HYPH 10_1101-2021_01_07_425723 290 6 deletion deletion NN 10_1101-2021_01_07_425723 290 7 strain strain NN 10_1101-2021_01_07_425723 290 8 was be VBD 10_1101-2021_01_07_425723 290 9 similarly similarly RB 10_1101-2021_01_07_425723 290 10 constructed construct VBN 10_1101-2021_01_07_425723 290 11 by by IN 10_1101-2021_01_07_425723 290 12 chemical chemical JJ 10_1101-2021_01_07_425723 290 13 transformation transformation NN 10_1101-2021_01_07_425723 290 14 of of IN 10_1101-2021_01_07_425723 290 15 431 431 CD 10_1101-2021_01_07_425723 290 16 S. S. NNP 10_1101-2021_01_07_425723 290 17 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 290 18 IMK802 IMK802 NNP 10_1101-2021_01_07_425723 290 19 with with IN 10_1101-2021_01_07_425723 290 20 pUDE663 pUDE663 NNP 10_1101-2021_01_07_425723 290 21 and and CC 10_1101-2021_01_07_425723 290 22 repair repair NN 10_1101-2021_01_07_425723 290 23 DNA dna NN 10_1101-2021_01_07_425723 290 24 . . . 10_1101-2021_01_07_425723 291 1 To to TO 10_1101-2021_01_07_425723 291 2 integrate integrate VB 10_1101-2021_01_07_425723 291 3 a a DT 10_1101-2021_01_07_425723 291 4 TtSTC1 ttstc1 JJ 10_1101-2021_01_07_425723 291 5 expression expression NN 10_1101-2021_01_07_425723 291 6 cassette cassette NN 10_1101-2021_01_07_425723 291 7 into into IN 10_1101-2021_01_07_425723 291 8 the the DT 10_1101-2021_01_07_425723 291 9 432 432 CD 10_1101-2021_01_07_425723 291 10 K. K. NNP 10_1101-2021_01_07_425723 291 11 marxianus marxianus NNP 10_1101-2021_01_07_425723 291 12 lac4 lac4 NNP 10_1101-2021_01_07_425723 291 13 locus locus NN 10_1101-2021_01_07_425723 291 14 , , , 10_1101-2021_01_07_425723 291 15 K. K. NNP 10_1101-2021_01_07_425723 291 16 marxianus marxianus NN 10_1101-2021_01_07_425723 291 17 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 291 18 was be VBD 10_1101-2021_01_07_425723 291 19 transformed transform VBN 10_1101-2021_01_07_425723 291 20 with with IN 10_1101-2021_01_07_425723 291 21 2 2 CD 10_1101-2021_01_07_425723 291 22 μg μg , 10_1101-2021_01_07_425723 291 23 DNA DNA NNP 10_1101-2021_01_07_425723 291 24 NotI NotI NNP 10_1101-2021_01_07_425723 291 25 - - HYPH 10_1101-2021_01_07_425723 291 26 digested digest VBN 10_1101-2021_01_07_425723 291 27 433 433 CD 10_1101-2021_01_07_425723 291 28 pUDI246 pUDI246 NNP 10_1101-2021_01_07_425723 291 29 . . . 10_1101-2021_01_07_425723 292 1 After after IN 10_1101-2021_01_07_425723 292 2 centrifugation centrifugation NN 10_1101-2021_01_07_425723 292 3 , , , 10_1101-2021_01_07_425723 292 4 cells cell NNS 10_1101-2021_01_07_425723 292 5 were be VBD 10_1101-2021_01_07_425723 292 6 resuspended resuspend VBN 10_1101-2021_01_07_425723 292 7 in in IN 10_1101-2021_01_07_425723 292 8 YPD YPD NNP 10_1101-2021_01_07_425723 292 9 and and CC 10_1101-2021_01_07_425723 292 10 incubated incubate VBD 10_1101-2021_01_07_425723 292 11 at at IN 10_1101-2021_01_07_425723 292 12 30 30 CD 10_1101-2021_01_07_425723 292 13 ° ° , 10_1101-2021_01_07_425723 292 14 C C NNP 10_1101-2021_01_07_425723 292 15 for for IN 10_1101-2021_01_07_425723 292 16 3 3 CD 10_1101-2021_01_07_425723 292 17 h. h. NN 10_1101-2021_01_07_425723 292 18 Cells Cells NNPS 10_1101-2021_01_07_425723 292 19 were be VBD 10_1101-2021_01_07_425723 292 20 434 434 CD 10_1101-2021_01_07_425723 292 21 then then RB 10_1101-2021_01_07_425723 292 22 again again RB 10_1101-2021_01_07_425723 292 23 centrifuged centrifuge VBN 10_1101-2021_01_07_425723 292 24 , , , 10_1101-2021_01_07_425723 292 25 resuspended resuspend VBN 10_1101-2021_01_07_425723 292 26 in in IN 10_1101-2021_01_07_425723 292 27 demineralized demineralized JJ 10_1101-2021_01_07_425723 292 28 water water NN 10_1101-2021_01_07_425723 292 29 and and CC 10_1101-2021_01_07_425723 292 30 plated plate VBN 10_1101-2021_01_07_425723 292 31 on on IN 10_1101-2021_01_07_425723 292 32 200 200 CD 10_1101-2021_01_07_425723 292 33 µg·L-1 µg·l-1 JJ 10_1101-2021_01_07_425723 292 34 hygromycin hygromycin NN 10_1101-2021_01_07_425723 292 35 B B NNP 10_1101-2021_01_07_425723 292 36 435 435 CD 10_1101-2021_01_07_425723 292 37 ( ( -LRB- 10_1101-2021_01_07_425723 292 38 InvivoGen invivogen NN 10_1101-2021_01_07_425723 292 39 , , , 10_1101-2021_01_07_425723 292 40 Toulouse Toulouse NNP 10_1101-2021_01_07_425723 292 41 , , , 10_1101-2021_01_07_425723 292 42 France France NNP 10_1101-2021_01_07_425723 292 43 ) ) -RRB- 10_1101-2021_01_07_425723 292 44 containing contain VBG 10_1101-2021_01_07_425723 292 45 agar agar NN 10_1101-2021_01_07_425723 292 46 with with IN 10_1101-2021_01_07_425723 292 47 40 40 CD 10_1101-2021_01_07_425723 292 48 µg·L-1 µg·L-1 NNP 10_1101-2021_01_07_425723 292 49 X x NN 10_1101-2021_01_07_425723 292 50 - - NN 10_1101-2021_01_07_425723 292 51 gal gal NNP 10_1101-2021_01_07_425723 292 52 , , , 10_1101-2021_01_07_425723 292 53 5-bromo-4-chloro-3-indolyl 5-bromo-4-chloro-3-indolyl CD 10_1101-2021_01_07_425723 292 54 - - HYPH 10_1101-2021_01_07_425723 292 55 β β FW 10_1101-2021_01_07_425723 292 56 - - HYPH 10_1101-2021_01_07_425723 292 57 D-436 D-436 NNP 10_1101-2021_01_07_425723 292 58 galactopyranoside galactopyranoside NN 10_1101-2021_01_07_425723 292 59 ( ( -LRB- 10_1101-2021_01_07_425723 292 60 Fermentas Fermentas NNP 10_1101-2021_01_07_425723 292 61 , , , 10_1101-2021_01_07_425723 292 62 Waltham Waltham NNP 10_1101-2021_01_07_425723 292 63 , , , 10_1101-2021_01_07_425723 292 64 MA MA NNP 10_1101-2021_01_07_425723 292 65 ) ) -RRB- 10_1101-2021_01_07_425723 292 66 . . . 10_1101-2021_01_07_425723 293 1 Colonies colony NNS 10_1101-2021_01_07_425723 293 2 that that WDT 10_1101-2021_01_07_425723 293 3 could could MD 10_1101-2021_01_07_425723 293 4 not not RB 10_1101-2021_01_07_425723 293 5 convert convert VB 10_1101-2021_01_07_425723 293 6 X x NN 10_1101-2021_01_07_425723 293 7 - - NN 10_1101-2021_01_07_425723 293 8 gal gal NNP 10_1101-2021_01_07_425723 293 9 were be VBD 10_1101-2021_01_07_425723 293 10 analyzed analyze VBN 10_1101-2021_01_07_425723 293 11 for for IN 10_1101-2021_01_07_425723 293 12 437 437 CD 10_1101-2021_01_07_425723 293 13 correct correct JJ 10_1101-2021_01_07_425723 293 14 genomic genomic JJ 10_1101-2021_01_07_425723 293 15 integration integration NN 10_1101-2021_01_07_425723 293 16 of of IN 10_1101-2021_01_07_425723 293 17 the the DT 10_1101-2021_01_07_425723 293 18 TtSTC1 ttstc1 NN 10_1101-2021_01_07_425723 293 19 by by IN 10_1101-2021_01_07_425723 293 20 diagnostic diagnostic JJ 10_1101-2021_01_07_425723 293 21 PCR PCR NNP 10_1101-2021_01_07_425723 293 22 with with IN 10_1101-2021_01_07_425723 293 23 primers primer NNS 10_1101-2021_01_07_425723 293 24 16366 16366 CD 10_1101-2021_01_07_425723 293 25 , , , 10_1101-2021_01_07_425723 293 26 16367 16367 CD 10_1101-2021_01_07_425723 293 27 and and CC 10_1101-2021_01_07_425723 293 28 11897 11897 CD 10_1101-2021_01_07_425723 293 29 . . . 10_1101-2021_01_07_425723 294 1 438 438 CD 10_1101-2021_01_07_425723 294 2 Genomic genomic JJ 10_1101-2021_01_07_425723 294 3 integration integration NN 10_1101-2021_01_07_425723 294 4 of of IN 10_1101-2021_01_07_425723 294 5 TtSTC1 ttstc1 NN 10_1101-2021_01_07_425723 294 6 into into IN 10_1101-2021_01_07_425723 294 7 the the DT 10_1101-2021_01_07_425723 294 8 chromosome chromosome NN 10_1101-2021_01_07_425723 294 9 outside outside IN 10_1101-2021_01_07_425723 294 10 the the DT 10_1101-2021_01_07_425723 294 11 lac4 lac4 NNP 10_1101-2021_01_07_425723 294 12 locus locus NN 10_1101-2021_01_07_425723 294 13 was be VBD 10_1101-2021_01_07_425723 294 14 confirmed confirm VBN 10_1101-2021_01_07_425723 294 15 by by IN 10_1101-2021_01_07_425723 294 16 short short JJ 10_1101-2021_01_07_425723 294 17 - - HYPH 10_1101-2021_01_07_425723 294 18 read read VBN 10_1101-2021_01_07_425723 294 19 439 439 CD 10_1101-2021_01_07_425723 294 20 Illumina Illumina NNP 10_1101-2021_01_07_425723 294 21 sequencing sequencing NN 10_1101-2021_01_07_425723 294 22 . . . 10_1101-2021_01_07_425723 295 1 440 440 CD 10_1101-2021_01_07_425723 295 2 Chemostat chemostat JJ 10_1101-2021_01_07_425723 295 3 cultivation cultivation NN 10_1101-2021_01_07_425723 295 4 441 441 CD 10_1101-2021_01_07_425723 295 5 Chemostat chemostat JJ 10_1101-2021_01_07_425723 295 6 cultures culture NNS 10_1101-2021_01_07_425723 295 7 were be VBD 10_1101-2021_01_07_425723 295 8 grown grow VBN 10_1101-2021_01_07_425723 295 9 at at IN 10_1101-2021_01_07_425723 295 10 30 30 CD 10_1101-2021_01_07_425723 295 11 ° ° , 10_1101-2021_01_07_425723 295 12 C C NNP 10_1101-2021_01_07_425723 295 13 in in IN 10_1101-2021_01_07_425723 295 14 2 2 CD 10_1101-2021_01_07_425723 295 15 L l NN 10_1101-2021_01_07_425723 295 16 bioreactors bioreactor NNS 10_1101-2021_01_07_425723 295 17 ( ( -LRB- 10_1101-2021_01_07_425723 295 18 Applikon Applikon NNP 10_1101-2021_01_07_425723 295 19 , , , 10_1101-2021_01_07_425723 295 20 Delft Delft NNP 10_1101-2021_01_07_425723 295 21 , , , 10_1101-2021_01_07_425723 295 22 the the DT 10_1101-2021_01_07_425723 295 23 Netherlands Netherlands NNP 10_1101-2021_01_07_425723 295 24 ) ) -RRB- 10_1101-2021_01_07_425723 295 25 with with IN 10_1101-2021_01_07_425723 295 26 a a DT 10_1101-2021_01_07_425723 295 27 442 442 CD 10_1101-2021_01_07_425723 295 28 stirrer stirrer NN 10_1101-2021_01_07_425723 295 29 speed speed NN 10_1101-2021_01_07_425723 295 30 of of IN 10_1101-2021_01_07_425723 295 31 800 800 CD 10_1101-2021_01_07_425723 295 32 rpm rpm NN 10_1101-2021_01_07_425723 295 33 . . . 10_1101-2021_01_07_425723 296 1 The the DT 10_1101-2021_01_07_425723 296 2 dilution dilution NN 10_1101-2021_01_07_425723 296 3 rate rate NN 10_1101-2021_01_07_425723 296 4 was be VBD 10_1101-2021_01_07_425723 296 5 set set VBN 10_1101-2021_01_07_425723 296 6 at at IN 10_1101-2021_01_07_425723 296 7 0.10 0.10 CD 10_1101-2021_01_07_425723 296 8 h-1 h-1 NNP 10_1101-2021_01_07_425723 296 9 and and CC 10_1101-2021_01_07_425723 296 10 a a DT 10_1101-2021_01_07_425723 296 11 constant constant JJ 10_1101-2021_01_07_425723 296 12 working work VBG 10_1101-2021_01_07_425723 296 13 volume volume NN 10_1101-2021_01_07_425723 296 14 of of IN 10_1101-2021_01_07_425723 296 15 1.2 1.2 CD 10_1101-2021_01_07_425723 296 16 L L NNP 10_1101-2021_01_07_425723 296 17 443 443 CD 10_1101-2021_01_07_425723 296 18 was be VBD 10_1101-2021_01_07_425723 296 19 maintained maintain VBN 10_1101-2021_01_07_425723 296 20 by by IN 10_1101-2021_01_07_425723 296 21 connecting connect VBG 10_1101-2021_01_07_425723 296 22 the the DT 10_1101-2021_01_07_425723 296 23 effluent effluent JJ 10_1101-2021_01_07_425723 296 24 pump pump NN 10_1101-2021_01_07_425723 296 25 to to IN 10_1101-2021_01_07_425723 296 26 a a DT 10_1101-2021_01_07_425723 296 27 level level NN 10_1101-2021_01_07_425723 296 28 sensor sensor NN 10_1101-2021_01_07_425723 296 29 . . . 10_1101-2021_01_07_425723 297 1 Cultures culture NNS 10_1101-2021_01_07_425723 297 2 were be VBD 10_1101-2021_01_07_425723 297 3 grown grow VBN 10_1101-2021_01_07_425723 297 4 on on IN 10_1101-2021_01_07_425723 297 5 synthetic synthetic JJ 10_1101-2021_01_07_425723 297 6 444 444 CD 10_1101-2021_01_07_425723 297 7 medium medium NN 10_1101-2021_01_07_425723 297 8 with with IN 10_1101-2021_01_07_425723 297 9 vitamins17 vitamins17 NN 10_1101-2021_01_07_425723 297 10 . . . 10_1101-2021_01_07_425723 298 1 Concentrated concentrated JJ 10_1101-2021_01_07_425723 298 2 glucose glucose NN 10_1101-2021_01_07_425723 298 3 solutions solution NNS 10_1101-2021_01_07_425723 298 4 were be VBD 10_1101-2021_01_07_425723 298 5 autoclaved autoclave VBN 10_1101-2021_01_07_425723 298 6 separately separately RB 10_1101-2021_01_07_425723 298 7 at at IN 10_1101-2021_01_07_425723 298 8 110 110 CD 10_1101-2021_01_07_425723 298 9 ° ° , 10_1101-2021_01_07_425723 298 10 C C NNP 10_1101-2021_01_07_425723 298 11 for for IN 10_1101-2021_01_07_425723 298 12 20 20 CD 10_1101-2021_01_07_425723 298 13 445 445 CD 10_1101-2021_01_07_425723 298 14 min min NN 10_1101-2021_01_07_425723 298 15 and and CC 10_1101-2021_01_07_425723 298 16 added add VBN 10_1101-2021_01_07_425723 298 17 at at IN 10_1101-2021_01_07_425723 298 18 the the DT 10_1101-2021_01_07_425723 298 19 concentrations concentration NNS 10_1101-2021_01_07_425723 298 20 indicated indicate VBN 10_1101-2021_01_07_425723 298 21 , , , 10_1101-2021_01_07_425723 298 22 along along IN 10_1101-2021_01_07_425723 298 23 with with IN 10_1101-2021_01_07_425723 298 24 sterile sterile JJ 10_1101-2021_01_07_425723 298 25 antifoam antifoam NNP 10_1101-2021_01_07_425723 298 26 pluronic pluronic NNP 10_1101-2021_01_07_425723 298 27 6100 6100 CD 10_1101-2021_01_07_425723 298 28 PE PE NNP 10_1101-2021_01_07_425723 298 29 ( ( -LRB- 10_1101-2021_01_07_425723 298 30 BASF BASF NNP 10_1101-2021_01_07_425723 298 31 , , , 10_1101-2021_01_07_425723 298 32 446 446 CD 10_1101-2021_01_07_425723 298 33 Ludwigshafen Ludwigshafen NNP 10_1101-2021_01_07_425723 298 34 , , , 10_1101-2021_01_07_425723 298 35 Germany Germany NNP 10_1101-2021_01_07_425723 298 36 ; ; : 10_1101-2021_01_07_425723 298 37 final final JJ 10_1101-2021_01_07_425723 298 38 concentration concentration NN 10_1101-2021_01_07_425723 298 39 0.2 0.2 CD 10_1101-2021_01_07_425723 298 40 g·L-1 g·L-1 NNP 10_1101-2021_01_07_425723 298 41 ) ) -RRB- 10_1101-2021_01_07_425723 298 42 . . . 10_1101-2021_01_07_425723 299 1 Before before IN 10_1101-2021_01_07_425723 299 2 autoclaving autoclaving NN 10_1101-2021_01_07_425723 299 3 , , , 10_1101-2021_01_07_425723 299 4 bioreactors bioreactor NNS 10_1101-2021_01_07_425723 299 5 were be VBD 10_1101-2021_01_07_425723 299 6 tested test VBN 10_1101-2021_01_07_425723 299 7 for for IN 10_1101-2021_01_07_425723 299 8 447 447 CD 10_1101-2021_01_07_425723 299 9 gas gas NN 10_1101-2021_01_07_425723 299 10 leakage leakage NN 10_1101-2021_01_07_425723 299 11 by by IN 10_1101-2021_01_07_425723 299 12 submerging submerge VBG 10_1101-2021_01_07_425723 299 13 them -PRON- PRP 10_1101-2021_01_07_425723 299 14 in in IN 10_1101-2021_01_07_425723 299 15 water water NN 10_1101-2021_01_07_425723 299 16 while while IN 10_1101-2021_01_07_425723 299 17 applying apply VBG 10_1101-2021_01_07_425723 299 18 a a DT 10_1101-2021_01_07_425723 299 19 0.3 0.3 CD 10_1101-2021_01_07_425723 299 20 bar bar NN 10_1101-2021_01_07_425723 299 21 overpressure overpressure NN 10_1101-2021_01_07_425723 299 22 . . . 10_1101-2021_01_07_425723 300 1 448 448 CD 10_1101-2021_01_07_425723 300 2 Anaerobic anaerobic JJ 10_1101-2021_01_07_425723 300 3 conditions condition NNS 10_1101-2021_01_07_425723 300 4 of of IN 10_1101-2021_01_07_425723 300 5 bioreactor bioreactor NN 10_1101-2021_01_07_425723 300 6 cultivations cultivation NNS 10_1101-2021_01_07_425723 300 7 were be VBD 10_1101-2021_01_07_425723 300 8 maintained maintain VBN 10_1101-2021_01_07_425723 300 9 by by IN 10_1101-2021_01_07_425723 300 10 continuous continuous JJ 10_1101-2021_01_07_425723 300 11 reactor reactor NN 10_1101-2021_01_07_425723 300 12 headspace headspace NN 10_1101-2021_01_07_425723 300 13 449 449 CD 10_1101-2021_01_07_425723 300 14 aeration aeration NN 10_1101-2021_01_07_425723 300 15 with with IN 10_1101-2021_01_07_425723 300 16 pure pure JJ 10_1101-2021_01_07_425723 300 17 nitrogen nitrogen NN 10_1101-2021_01_07_425723 300 18 gas gas NN 10_1101-2021_01_07_425723 300 19 ( ( -LRB- 10_1101-2021_01_07_425723 300 20 ≤ ≤ NNP 10_1101-2021_01_07_425723 300 21 0.5 0.5 CD 10_1101-2021_01_07_425723 300 22 ppm ppm NN 10_1101-2021_01_07_425723 300 23 O2 O2 VBN 10_1101-2021_01_07_425723 300 24 , , , 10_1101-2021_01_07_425723 300 25 HiQ HiQ NNP 10_1101-2021_01_07_425723 300 26 Nitrogen Nitrogen NNP 10_1101-2021_01_07_425723 300 27 6.0 6.0 CD 10_1101-2021_01_07_425723 300 28 , , , 10_1101-2021_01_07_425723 300 29 Linde Linde NNP 10_1101-2021_01_07_425723 300 30 AG AG NNP 10_1101-2021_01_07_425723 300 31 , , , 10_1101-2021_01_07_425723 300 32 Schiedam Schiedam NNP 10_1101-2021_01_07_425723 300 33 , , , 10_1101-2021_01_07_425723 300 34 the the DT 10_1101-2021_01_07_425723 300 35 Netherlands Netherlands NNP 10_1101-2021_01_07_425723 300 36 ) ) -RRB- 10_1101-2021_01_07_425723 300 37 450 450 CD 10_1101-2021_01_07_425723 300 38 .CC .CC : 10_1101-2021_01_07_425723 300 39 - - HYPH 10_1101-2021_01_07_425723 300 40 BY by IN 10_1101-2021_01_07_425723 300 41 - - HYPH 10_1101-2021_01_07_425723 300 42 NC NC NNP 10_1101-2021_01_07_425723 300 43 - - HYPH 10_1101-2021_01_07_425723 300 44 ND ND NNP 10_1101-2021_01_07_425723 300 45 4.0 4.0 CD 10_1101-2021_01_07_425723 300 46 International International NNP 10_1101-2021_01_07_425723 300 47 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 300 48 under under IN 10_1101-2021_01_07_425723 300 49 a a DT 10_1101-2021_01_07_425723 300 50 ( ( -LRB- 10_1101-2021_01_07_425723 300 51 which which WDT 10_1101-2021_01_07_425723 300 52 was be VBD 10_1101-2021_01_07_425723 300 53 not not RB 10_1101-2021_01_07_425723 300 54 certified certify VBN 10_1101-2021_01_07_425723 300 55 by by IN 10_1101-2021_01_07_425723 300 56 peer peer NN 10_1101-2021_01_07_425723 300 57 review review NN 10_1101-2021_01_07_425723 300 58 ) ) -RRB- 10_1101-2021_01_07_425723 300 59 is be VBZ 10_1101-2021_01_07_425723 300 60 the the DT 10_1101-2021_01_07_425723 300 61 author author NN 10_1101-2021_01_07_425723 300 62 / / SYM 10_1101-2021_01_07_425723 300 63 funder funder NN 10_1101-2021_01_07_425723 300 64 , , , 10_1101-2021_01_07_425723 300 65 who who WP 10_1101-2021_01_07_425723 300 66 has have VBZ 10_1101-2021_01_07_425723 300 67 granted grant VBN 10_1101-2021_01_07_425723 300 68 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 300 69 a a DT 10_1101-2021_01_07_425723 300 70 license license NN 10_1101-2021_01_07_425723 300 71 to to TO 10_1101-2021_01_07_425723 300 72 display display VB 10_1101-2021_01_07_425723 300 73 the the DT 10_1101-2021_01_07_425723 300 74 preprint preprint NN 10_1101-2021_01_07_425723 300 75 in in IN 10_1101-2021_01_07_425723 300 76 perpetuity perpetuity NN 10_1101-2021_01_07_425723 300 77 . . . 10_1101-2021_01_07_425723 301 1 It -PRON- PRP 10_1101-2021_01_07_425723 301 2 is be VBZ 10_1101-2021_01_07_425723 301 3 made make VBN 10_1101-2021_01_07_425723 301 4 The the DT 10_1101-2021_01_07_425723 301 5 copyright copyright NN 10_1101-2021_01_07_425723 301 6 holder holder NN 10_1101-2021_01_07_425723 301 7 for for IN 10_1101-2021_01_07_425723 301 8 this this DT 10_1101-2021_01_07_425723 301 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 301 10 version version NN 10_1101-2021_01_07_425723 301 11 posted post VBD 10_1101-2021_01_07_425723 301 12 January January NNP 10_1101-2021_01_07_425723 301 13 8 8 CD 10_1101-2021_01_07_425723 301 14 , , , 10_1101-2021_01_07_425723 301 15 2021 2021 CD 10_1101-2021_01_07_425723 301 16 . . . 10_1101-2021_01_07_425723 301 17 ; ; : 10_1101-2021_01_07_425723 301 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 301 19 : : : 10_1101-2021_01_07_425723 301 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 301 21 preprint preprint NN 10_1101-2021_01_07_425723 301 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 301 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425723 301 24 28 28 CD 10_1101-2021_01_07_425723 301 25 at at IN 10_1101-2021_01_07_425723 301 26 a a DT 10_1101-2021_01_07_425723 301 27 flowrate flowrate NN 10_1101-2021_01_07_425723 301 28 of of IN 10_1101-2021_01_07_425723 301 29 500 500 CD 10_1101-2021_01_07_425723 301 30 mL mL NNP 10_1101-2021_01_07_425723 301 31 N2 N2 NNP 10_1101-2021_01_07_425723 301 32 min-1 min-1 NNP 10_1101-2021_01_07_425723 301 33 ( ( -LRB- 10_1101-2021_01_07_425723 301 34 2.4 2.4 CD 10_1101-2021_01_07_425723 301 35 vvm vvm NN 10_1101-2021_01_07_425723 301 36 ) ) -RRB- 10_1101-2021_01_07_425723 301 37 . . . 10_1101-2021_01_07_425723 302 1 Gas gas NN 10_1101-2021_01_07_425723 302 2 pressure pressure NN 10_1101-2021_01_07_425723 302 3 of of IN 10_1101-2021_01_07_425723 302 4 1.2 1.2 CD 10_1101-2021_01_07_425723 302 5 bar bar NN 10_1101-2021_01_07_425723 302 6 of of IN 10_1101-2021_01_07_425723 302 7 the the DT 10_1101-2021_01_07_425723 302 8 reactor reactor NN 10_1101-2021_01_07_425723 302 9 headspace headspace NN 10_1101-2021_01_07_425723 302 10 was be VBD 10_1101-2021_01_07_425723 302 11 set set VBN 10_1101-2021_01_07_425723 302 12 with with IN 10_1101-2021_01_07_425723 302 13 451 451 CD 10_1101-2021_01_07_425723 302 14 a a DT 10_1101-2021_01_07_425723 302 15 reduction reduction NN 10_1101-2021_01_07_425723 302 16 valve valve NN 10_1101-2021_01_07_425723 302 17 ( ( -LRB- 10_1101-2021_01_07_425723 302 18 Tescom Tescom NNP 10_1101-2021_01_07_425723 302 19 Europe Europe NNP 10_1101-2021_01_07_425723 302 20 , , , 10_1101-2021_01_07_425723 302 21 Hannover Hannover NNP 10_1101-2021_01_07_425723 302 22 , , , 10_1101-2021_01_07_425723 302 23 Germany Germany NNP 10_1101-2021_01_07_425723 302 24 ) ) -RRB- 10_1101-2021_01_07_425723 302 25 and and CC 10_1101-2021_01_07_425723 302 26 remained remain VBD 10_1101-2021_01_07_425723 302 27 constant constant JJ 10_1101-2021_01_07_425723 302 28 during during IN 10_1101-2021_01_07_425723 302 29 cultivation cultivation NN 10_1101-2021_01_07_425723 302 30 . . . 10_1101-2021_01_07_425723 303 1 To to IN 10_1101-2021_01_07_425723 303 2 452 452 CD 10_1101-2021_01_07_425723 303 3 prevent prevent VB 10_1101-2021_01_07_425723 303 4 oxygen oxygen NN 10_1101-2021_01_07_425723 303 5 diffusion diffusion NN 10_1101-2021_01_07_425723 303 6 into into IN 10_1101-2021_01_07_425723 303 7 the the DT 10_1101-2021_01_07_425723 303 8 cultivation cultivation NN 10_1101-2021_01_07_425723 303 9 the the DT 10_1101-2021_01_07_425723 303 10 bioreactor bioreactor NN 10_1101-2021_01_07_425723 303 11 was be VBD 10_1101-2021_01_07_425723 303 12 equipped equip VBN 10_1101-2021_01_07_425723 303 13 with with IN 10_1101-2021_01_07_425723 303 14 Fluran fluran JJ 10_1101-2021_01_07_425723 303 15 tubing tubing NN 10_1101-2021_01_07_425723 303 16 ( ( -LRB- 10_1101-2021_01_07_425723 303 17 14 14 CD 10_1101-2021_01_07_425723 303 18 Barrer Barrer NNP 10_1101-2021_01_07_425723 303 19 453 453 CD 10_1101-2021_01_07_425723 303 20 O2 o2 CD 10_1101-2021_01_07_425723 303 21 , , , 10_1101-2021_01_07_425723 303 22 F-5500-A f-5500-a NN 10_1101-2021_01_07_425723 303 23 , , , 10_1101-2021_01_07_425723 303 24 Saint Saint NNP 10_1101-2021_01_07_425723 303 25 - - HYPH 10_1101-2021_01_07_425723 303 26 Gobain Gobain NNP 10_1101-2021_01_07_425723 303 27 , , , 10_1101-2021_01_07_425723 303 28 Courbevoie Courbevoie NNP 10_1101-2021_01_07_425723 303 29 , , , 10_1101-2021_01_07_425723 303 30 France France NNP 10_1101-2021_01_07_425723 303 31 ) ) -RRB- 10_1101-2021_01_07_425723 303 32 , , , 10_1101-2021_01_07_425723 303 33 Viton Viton NNP 10_1101-2021_01_07_425723 303 34 O O NNP 10_1101-2021_01_07_425723 303 35 - - HYPH 10_1101-2021_01_07_425723 303 36 rings ring NNS 10_1101-2021_01_07_425723 303 37 ( ( -LRB- 10_1101-2021_01_07_425723 303 38 Eriks Eriks NNP 10_1101-2021_01_07_425723 303 39 , , , 10_1101-2021_01_07_425723 303 40 Alkmaar Alkmaar NNP 10_1101-2021_01_07_425723 303 41 , , , 10_1101-2021_01_07_425723 303 42 the the DT 10_1101-2021_01_07_425723 303 43 Netherlands Netherlands NNP 10_1101-2021_01_07_425723 303 44 ) ) -RRB- 10_1101-2021_01_07_425723 303 45 , , , 10_1101-2021_01_07_425723 303 46 and and CC 10_1101-2021_01_07_425723 303 47 no no DT 10_1101-2021_01_07_425723 303 48 454 454 CD 10_1101-2021_01_07_425723 303 49 pH pH NNP 10_1101-2021_01_07_425723 303 50 probes probe NNS 10_1101-2021_01_07_425723 303 51 were be VBD 10_1101-2021_01_07_425723 303 52 mounted mount VBN 10_1101-2021_01_07_425723 303 53 . . . 10_1101-2021_01_07_425723 304 1 The the DT 10_1101-2021_01_07_425723 304 2 medium medium JJ 10_1101-2021_01_07_425723 304 3 reservoir reservoir NN 10_1101-2021_01_07_425723 304 4 was be VBD 10_1101-2021_01_07_425723 304 5 deoxygenated deoxygenate VBN 10_1101-2021_01_07_425723 304 6 by by IN 10_1101-2021_01_07_425723 304 7 sparge sparge NN 10_1101-2021_01_07_425723 304 8 aeration aeration NN 10_1101-2021_01_07_425723 304 9 with with IN 10_1101-2021_01_07_425723 304 10 nitrogen nitrogen NN 10_1101-2021_01_07_425723 304 11 455 455 CD 10_1101-2021_01_07_425723 304 12 gas gas NN 10_1101-2021_01_07_425723 304 13 ( ( -LRB- 10_1101-2021_01_07_425723 304 14 ≤ ≤ NNP 10_1101-2021_01_07_425723 304 15 1 1 CD 10_1101-2021_01_07_425723 304 16 ppm ppm NN 10_1101-2021_01_07_425723 304 17 O2 O2 NNP 10_1101-2021_01_07_425723 304 18 , , , 10_1101-2021_01_07_425723 304 19 HiQ HiQ NNP 10_1101-2021_01_07_425723 304 20 Nitrogen Nitrogen NNP 10_1101-2021_01_07_425723 304 21 5.0 5.0 CD 10_1101-2021_01_07_425723 304 22 , , , 10_1101-2021_01_07_425723 304 23 Linde Linde NNP 10_1101-2021_01_07_425723 304 24 AG AG NNP 10_1101-2021_01_07_425723 304 25 ) ) -RRB- 10_1101-2021_01_07_425723 304 26 . . . 10_1101-2021_01_07_425723 305 1 456 456 CD 10_1101-2021_01_07_425723 305 2 For for IN 10_1101-2021_01_07_425723 305 3 aerobic aerobic JJ 10_1101-2021_01_07_425723 305 4 cultivation cultivation NN 10_1101-2021_01_07_425723 305 5 the the DT 10_1101-2021_01_07_425723 305 6 reactor reactor NN 10_1101-2021_01_07_425723 305 7 was be VBD 10_1101-2021_01_07_425723 305 8 sparged sparge VBN 10_1101-2021_01_07_425723 305 9 continuously continuously RB 10_1101-2021_01_07_425723 305 10 with with IN 10_1101-2021_01_07_425723 305 11 dried dry VBN 10_1101-2021_01_07_425723 305 12 air air NN 10_1101-2021_01_07_425723 305 13 at at IN 10_1101-2021_01_07_425723 305 14 a a DT 10_1101-2021_01_07_425723 305 15 flowrate flowrate NN 10_1101-2021_01_07_425723 305 16 of of IN 10_1101-2021_01_07_425723 305 17 500 500 CD 10_1101-2021_01_07_425723 305 18 mL mL NNP 10_1101-2021_01_07_425723 305 19 air air NN 10_1101-2021_01_07_425723 305 20 457 457 CD 10_1101-2021_01_07_425723 305 21 min-1 min-1 NNS 10_1101-2021_01_07_425723 305 22 ( ( -LRB- 10_1101-2021_01_07_425723 305 23 2.4 2.4 CD 10_1101-2021_01_07_425723 305 24 vvm vvm NN 10_1101-2021_01_07_425723 305 25 ) ) -RRB- 10_1101-2021_01_07_425723 305 26 . . . 10_1101-2021_01_07_425723 306 1 Dissolved dissolved JJ 10_1101-2021_01_07_425723 306 2 oxygen oxygen NN 10_1101-2021_01_07_425723 306 3 levels level NNS 10_1101-2021_01_07_425723 306 4 were be VBD 10_1101-2021_01_07_425723 306 5 analyzed analyze VBN 10_1101-2021_01_07_425723 306 6 by by IN 10_1101-2021_01_07_425723 306 7 Clark Clark NNP 10_1101-2021_01_07_425723 306 8 electrodes electrode NNS 10_1101-2021_01_07_425723 306 9 ( ( -LRB- 10_1101-2021_01_07_425723 306 10 AppliSens AppliSens NNP 10_1101-2021_01_07_425723 306 11 , , , 10_1101-2021_01_07_425723 306 12 Applikon Applikon NNP 10_1101-2021_01_07_425723 306 13 ) ) -RRB- 10_1101-2021_01_07_425723 306 14 and and CC 10_1101-2021_01_07_425723 306 15 458 458 CD 10_1101-2021_01_07_425723 306 16 remained remain VBD 10_1101-2021_01_07_425723 306 17 above above IN 10_1101-2021_01_07_425723 306 18 40 40 CD 10_1101-2021_01_07_425723 306 19 % % NN 10_1101-2021_01_07_425723 306 20 during during IN 10_1101-2021_01_07_425723 306 21 the the DT 10_1101-2021_01_07_425723 306 22 cultivation cultivation NN 10_1101-2021_01_07_425723 306 23 . . . 10_1101-2021_01_07_425723 307 1 For for IN 10_1101-2021_01_07_425723 307 2 micro micro JJ 10_1101-2021_01_07_425723 307 3 - - JJ 10_1101-2021_01_07_425723 307 4 aerobic aerobic JJ 10_1101-2021_01_07_425723 307 5 cultivations cultivation NNS 10_1101-2021_01_07_425723 307 6 nitrogen nitrogen NN 10_1101-2021_01_07_425723 307 7 ( ( -LRB- 10_1101-2021_01_07_425723 307 8 ≤ ≤ NNP 10_1101-2021_01_07_425723 307 9 1 1 CD 10_1101-2021_01_07_425723 307 10 ppm ppm NN 10_1101-2021_01_07_425723 307 11 O2 O2 NNP 10_1101-2021_01_07_425723 307 12 , , , 10_1101-2021_01_07_425723 307 13 HiQ HiQ NNP 10_1101-2021_01_07_425723 307 14 459 459 CD 10_1101-2021_01_07_425723 307 15 Nitrogen Nitrogen NNP 10_1101-2021_01_07_425723 307 16 5.0 5.0 CD 10_1101-2021_01_07_425723 307 17 , , , 10_1101-2021_01_07_425723 307 18 Linde Linde NNP 10_1101-2021_01_07_425723 307 19 AG AG NNP 10_1101-2021_01_07_425723 307 20 ) ) -RRB- 10_1101-2021_01_07_425723 307 21 and and CC 10_1101-2021_01_07_425723 307 22 air air NN 10_1101-2021_01_07_425723 307 23 were be VBD 10_1101-2021_01_07_425723 307 24 mixed mix VBN 10_1101-2021_01_07_425723 307 25 continuously continuously RB 10_1101-2021_01_07_425723 307 26 by by IN 10_1101-2021_01_07_425723 307 27 controlling control VBG 10_1101-2021_01_07_425723 307 28 the the DT 10_1101-2021_01_07_425723 307 29 fractions fraction NNS 10_1101-2021_01_07_425723 307 30 of of IN 10_1101-2021_01_07_425723 307 31 mass mass JJ 10_1101-2021_01_07_425723 307 32 flow flow NN 10_1101-2021_01_07_425723 307 33 rate rate NN 10_1101-2021_01_07_425723 307 34 of of IN 10_1101-2021_01_07_425723 307 35 460 460 CD 10_1101-2021_01_07_425723 307 36 the the DT 10_1101-2021_01_07_425723 307 37 dry dry JJ 10_1101-2021_01_07_425723 307 38 gas gas NN 10_1101-2021_01_07_425723 307 39 to to IN 10_1101-2021_01_07_425723 307 40 a a DT 10_1101-2021_01_07_425723 307 41 total total JJ 10_1101-2021_01_07_425723 307 42 flow flow NN 10_1101-2021_01_07_425723 307 43 of of IN 10_1101-2021_01_07_425723 307 44 500 500 CD 10_1101-2021_01_07_425723 307 45 mL mL NNP 10_1101-2021_01_07_425723 307 46 min-1 min-1 NNS 10_1101-2021_01_07_425723 307 47 per per IN 10_1101-2021_01_07_425723 307 48 bioreactor bioreactor NN 10_1101-2021_01_07_425723 307 49 . . . 10_1101-2021_01_07_425723 308 1 The the DT 10_1101-2021_01_07_425723 308 2 mixed mixed JJ 10_1101-2021_01_07_425723 308 3 gas gas NN 10_1101-2021_01_07_425723 308 4 was be VBD 10_1101-2021_01_07_425723 308 5 distributed distribute VBN 10_1101-2021_01_07_425723 308 6 to to IN 10_1101-2021_01_07_425723 308 7 each each DT 10_1101-2021_01_07_425723 308 8 461 461 CD 10_1101-2021_01_07_425723 308 9 bioreactor bioreactor NN 10_1101-2021_01_07_425723 308 10 and and CC 10_1101-2021_01_07_425723 308 11 analyzed analyze VBD 10_1101-2021_01_07_425723 308 12 separately separately RB 10_1101-2021_01_07_425723 308 13 in in IN 10_1101-2021_01_07_425723 308 14 real real JJ 10_1101-2021_01_07_425723 308 15 - - HYPH 10_1101-2021_01_07_425723 308 16 time time NN 10_1101-2021_01_07_425723 308 17 . . . 10_1101-2021_01_07_425723 309 1 Continuous continuous JJ 10_1101-2021_01_07_425723 309 2 cultures culture NNS 10_1101-2021_01_07_425723 309 3 were be VBD 10_1101-2021_01_07_425723 309 4 assumed assume VBN 10_1101-2021_01_07_425723 309 5 to to TO 10_1101-2021_01_07_425723 309 6 be be VB 10_1101-2021_01_07_425723 309 7 in in IN 10_1101-2021_01_07_425723 309 8 steady steady JJ 10_1101-2021_01_07_425723 309 9 state state NN 10_1101-2021_01_07_425723 309 10 462 462 CD 10_1101-2021_01_07_425723 309 11 when when WRB 10_1101-2021_01_07_425723 309 12 after after IN 10_1101-2021_01_07_425723 309 13 at at RB 10_1101-2021_01_07_425723 309 14 least least JJS 10_1101-2021_01_07_425723 309 15 5 5 CD 10_1101-2021_01_07_425723 309 16 volumes volume NNS 10_1101-2021_01_07_425723 309 17 changes change NNS 10_1101-2021_01_07_425723 309 18 , , , 10_1101-2021_01_07_425723 309 19 culture culture NN 10_1101-2021_01_07_425723 309 20 dry dry JJ 10_1101-2021_01_07_425723 309 21 weight weight NN 10_1101-2021_01_07_425723 309 22 and and CC 10_1101-2021_01_07_425723 309 23 the the DT 10_1101-2021_01_07_425723 309 24 specific specific JJ 10_1101-2021_01_07_425723 309 25 carbon carbon NN 10_1101-2021_01_07_425723 309 26 dioxide dioxide NN 10_1101-2021_01_07_425723 309 27 production production NN 10_1101-2021_01_07_425723 309 28 463 463 CD 10_1101-2021_01_07_425723 309 29 rates rate NNS 10_1101-2021_01_07_425723 309 30 changed change VBN 10_1101-2021_01_07_425723 309 31 by by IN 10_1101-2021_01_07_425723 309 32 less less JJR 10_1101-2021_01_07_425723 309 33 than than IN 10_1101-2021_01_07_425723 309 34 10 10 CD 10_1101-2021_01_07_425723 309 35 % % NN 10_1101-2021_01_07_425723 309 36 . . . 10_1101-2021_01_07_425723 310 1 464 464 CD 10_1101-2021_01_07_425723 310 2 Cell Cell NNP 10_1101-2021_01_07_425723 310 3 density density NN 10_1101-2021_01_07_425723 310 4 was be VBD 10_1101-2021_01_07_425723 310 5 routinely routinely RB 10_1101-2021_01_07_425723 310 6 measured measure VBN 10_1101-2021_01_07_425723 310 7 at at IN 10_1101-2021_01_07_425723 310 8 a a DT 10_1101-2021_01_07_425723 310 9 wavelength wavelength NN 10_1101-2021_01_07_425723 310 10 of of IN 10_1101-2021_01_07_425723 310 11 660 660 CD 10_1101-2021_01_07_425723 310 12 nm nm NN 10_1101-2021_01_07_425723 310 13 with with IN 10_1101-2021_01_07_425723 310 14 spectrophotometer spectrophotometer NN 10_1101-2021_01_07_425723 310 15 Jenway Jenway NNP 10_1101-2021_01_07_425723 310 16 7200 7200 CD 10_1101-2021_01_07_425723 310 17 465 465 CD 10_1101-2021_01_07_425723 310 18 ( ( -LRB- 10_1101-2021_01_07_425723 310 19 Cole Cole NNP 10_1101-2021_01_07_425723 310 20 Palmer Palmer NNP 10_1101-2021_01_07_425723 310 21 , , , 10_1101-2021_01_07_425723 310 22 Staffordshire Staffordshire NNP 10_1101-2021_01_07_425723 310 23 , , , 10_1101-2021_01_07_425723 310 24 UK UK NNP 10_1101-2021_01_07_425723 310 25 ) ) -RRB- 10_1101-2021_01_07_425723 310 26 . . . 10_1101-2021_01_07_425723 311 1 Cell cell NN 10_1101-2021_01_07_425723 311 2 dry dry JJ 10_1101-2021_01_07_425723 311 3 weight weight NN 10_1101-2021_01_07_425723 311 4 of of IN 10_1101-2021_01_07_425723 311 5 the the DT 10_1101-2021_01_07_425723 311 6 cultures culture NNS 10_1101-2021_01_07_425723 311 7 were be VBD 10_1101-2021_01_07_425723 311 8 determined determine VBN 10_1101-2021_01_07_425723 311 9 by by IN 10_1101-2021_01_07_425723 311 10 filtering filter VBG 10_1101-2021_01_07_425723 311 11 exactly exactly RB 10_1101-2021_01_07_425723 311 12 10 10 CD 10_1101-2021_01_07_425723 311 13 466 466 CD 10_1101-2021_01_07_425723 311 14 mL ml NN 10_1101-2021_01_07_425723 311 15 of of IN 10_1101-2021_01_07_425723 311 16 culture culture NN 10_1101-2021_01_07_425723 311 17 broth broth NN 10_1101-2021_01_07_425723 311 18 over over IN 10_1101-2021_01_07_425723 311 19 pre pre JJ 10_1101-2021_01_07_425723 311 20 - - VBN 10_1101-2021_01_07_425723 311 21 dried dry VBN 10_1101-2021_01_07_425723 311 22 and and CC 10_1101-2021_01_07_425723 311 23 weighed weigh VBD 10_1101-2021_01_07_425723 311 24 membrane membrane NN 10_1101-2021_01_07_425723 311 25 filters filter NNS 10_1101-2021_01_07_425723 311 26 ( ( -LRB- 10_1101-2021_01_07_425723 311 27 0.45 0.45 CD 10_1101-2021_01_07_425723 311 28 µm µm NNP 10_1101-2021_01_07_425723 311 29 , , , 10_1101-2021_01_07_425723 311 30 Thermo Thermo NNP 10_1101-2021_01_07_425723 311 31 Fisher Fisher NNP 10_1101-2021_01_07_425723 311 32 Scientific Scientific NNP 10_1101-2021_01_07_425723 311 33 ) ) -RRB- 10_1101-2021_01_07_425723 311 34 , , , 10_1101-2021_01_07_425723 311 35 467 467 CD 10_1101-2021_01_07_425723 311 36 which which WDT 10_1101-2021_01_07_425723 311 37 were be VBD 10_1101-2021_01_07_425723 311 38 subsequently subsequently RB 10_1101-2021_01_07_425723 311 39 washed wash VBN 10_1101-2021_01_07_425723 311 40 with with IN 10_1101-2021_01_07_425723 311 41 demineralized demineralized JJ 10_1101-2021_01_07_425723 311 42 water water NN 10_1101-2021_01_07_425723 311 43 , , , 10_1101-2021_01_07_425723 311 44 dried dry VBN 10_1101-2021_01_07_425723 311 45 in in IN 10_1101-2021_01_07_425723 311 46 a a DT 10_1101-2021_01_07_425723 311 47 microwave microwave NN 10_1101-2021_01_07_425723 311 48 oven oven NN 10_1101-2021_01_07_425723 311 49 ( ( -LRB- 10_1101-2021_01_07_425723 311 50 20 20 CD 10_1101-2021_01_07_425723 311 51 min min NN 10_1101-2021_01_07_425723 311 52 , , , 10_1101-2021_01_07_425723 311 53 350 350 CD 10_1101-2021_01_07_425723 311 54 W w NN 10_1101-2021_01_07_425723 311 55 ) ) -RRB- 10_1101-2021_01_07_425723 311 56 468 468 CD 10_1101-2021_01_07_425723 311 57 and and CC 10_1101-2021_01_07_425723 311 58 weighed weigh VBD 10_1101-2021_01_07_425723 311 59 again66 again66 NNP 10_1101-2021_01_07_425723 311 60 . . . 10_1101-2021_01_07_425723 312 1 469 469 CD 10_1101-2021_01_07_425723 312 2 Metabolite metabolite JJ 10_1101-2021_01_07_425723 312 3 analysis analysis NN 10_1101-2021_01_07_425723 312 4 470 470 CD 10_1101-2021_01_07_425723 312 5 For for IN 10_1101-2021_01_07_425723 312 6 determination determination NN 10_1101-2021_01_07_425723 312 7 of of IN 10_1101-2021_01_07_425723 312 8 substrate substrate NN 10_1101-2021_01_07_425723 312 9 and and CC 10_1101-2021_01_07_425723 312 10 extracellular extracellular JJ 10_1101-2021_01_07_425723 312 11 metabolite metabolite JJ 10_1101-2021_01_07_425723 312 12 concentrations concentration NNS 10_1101-2021_01_07_425723 312 13 , , , 10_1101-2021_01_07_425723 312 14 culture culture NN 10_1101-2021_01_07_425723 312 15 supernatants supernatant NNS 10_1101-2021_01_07_425723 312 16 were be VBD 10_1101-2021_01_07_425723 312 17 471 471 CD 10_1101-2021_01_07_425723 312 18 obtained obtain VBN 10_1101-2021_01_07_425723 312 19 by by IN 10_1101-2021_01_07_425723 312 20 centrifugation centrifugation NN 10_1101-2021_01_07_425723 312 21 of of IN 10_1101-2021_01_07_425723 312 22 culture culture NN 10_1101-2021_01_07_425723 312 23 samples sample NNS 10_1101-2021_01_07_425723 312 24 ( ( -LRB- 10_1101-2021_01_07_425723 312 25 5 5 CD 10_1101-2021_01_07_425723 312 26 min min NN 10_1101-2021_01_07_425723 312 27 at at IN 10_1101-2021_01_07_425723 312 28 13000 13000 CD 10_1101-2021_01_07_425723 312 29 rpm rpm NNP 10_1101-2021_01_07_425723 312 30 ) ) -RRB- 10_1101-2021_01_07_425723 312 31 and and CC 10_1101-2021_01_07_425723 312 32 analyzed analyze VBN 10_1101-2021_01_07_425723 312 33 by by IN 10_1101-2021_01_07_425723 312 34 high high JJ 10_1101-2021_01_07_425723 312 35 - - HYPH 10_1101-2021_01_07_425723 312 36 performance performance NN 10_1101-2021_01_07_425723 312 37 472 472 CD 10_1101-2021_01_07_425723 312 38 liquid liquid JJ 10_1101-2021_01_07_425723 312 39 chromatography chromatography NN 10_1101-2021_01_07_425723 312 40 ( ( -LRB- 10_1101-2021_01_07_425723 312 41 HPLC HPLC NNP 10_1101-2021_01_07_425723 312 42 ) ) -RRB- 10_1101-2021_01_07_425723 312 43 on on IN 10_1101-2021_01_07_425723 312 44 a a DT 10_1101-2021_01_07_425723 312 45 Waters Waters NNP 10_1101-2021_01_07_425723 312 46 Alliance Alliance NNP 10_1101-2021_01_07_425723 312 47 2690 2690 CD 10_1101-2021_01_07_425723 312 48 HPLC hplc NN 10_1101-2021_01_07_425723 312 49 ( ( -LRB- 10_1101-2021_01_07_425723 312 50 Waters Waters NNP 10_1101-2021_01_07_425723 312 51 , , , 10_1101-2021_01_07_425723 312 52 MA MA NNP 10_1101-2021_01_07_425723 312 53 , , , 10_1101-2021_01_07_425723 312 54 USA USA NNP 10_1101-2021_01_07_425723 312 55 ) ) -RRB- 10_1101-2021_01_07_425723 312 56 equipped equip VBD 10_1101-2021_01_07_425723 312 57 with with IN 10_1101-2021_01_07_425723 312 58 a a DT 10_1101-2021_01_07_425723 312 59 Bio-473 bio-473 CD 10_1101-2021_01_07_425723 312 60 .CC .CC : 10_1101-2021_01_07_425723 312 61 - - : 10_1101-2021_01_07_425723 312 62 BY by IN 10_1101-2021_01_07_425723 312 63 - - HYPH 10_1101-2021_01_07_425723 312 64 NC NC NNP 10_1101-2021_01_07_425723 312 65 - - HYPH 10_1101-2021_01_07_425723 312 66 ND ND NNP 10_1101-2021_01_07_425723 312 67 4.0 4.0 CD 10_1101-2021_01_07_425723 312 68 International International NNP 10_1101-2021_01_07_425723 312 69 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 312 70 under under IN 10_1101-2021_01_07_425723 312 71 a a DT 10_1101-2021_01_07_425723 312 72 ( ( -LRB- 10_1101-2021_01_07_425723 312 73 which which WDT 10_1101-2021_01_07_425723 312 74 was be VBD 10_1101-2021_01_07_425723 312 75 not not RB 10_1101-2021_01_07_425723 312 76 certified certify VBN 10_1101-2021_01_07_425723 312 77 by by IN 10_1101-2021_01_07_425723 312 78 peer peer NN 10_1101-2021_01_07_425723 312 79 review review NN 10_1101-2021_01_07_425723 312 80 ) ) -RRB- 10_1101-2021_01_07_425723 312 81 is be VBZ 10_1101-2021_01_07_425723 312 82 the the DT 10_1101-2021_01_07_425723 312 83 author author NN 10_1101-2021_01_07_425723 312 84 / / SYM 10_1101-2021_01_07_425723 312 85 funder funder NN 10_1101-2021_01_07_425723 312 86 , , , 10_1101-2021_01_07_425723 312 87 who who WP 10_1101-2021_01_07_425723 312 88 has have VBZ 10_1101-2021_01_07_425723 312 89 granted grant VBN 10_1101-2021_01_07_425723 312 90 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 312 91 a a DT 10_1101-2021_01_07_425723 312 92 license license NN 10_1101-2021_01_07_425723 312 93 to to TO 10_1101-2021_01_07_425723 312 94 display display VB 10_1101-2021_01_07_425723 312 95 the the DT 10_1101-2021_01_07_425723 312 96 preprint preprint NN 10_1101-2021_01_07_425723 312 97 in in IN 10_1101-2021_01_07_425723 312 98 perpetuity perpetuity NN 10_1101-2021_01_07_425723 312 99 . . . 10_1101-2021_01_07_425723 313 1 It -PRON- PRP 10_1101-2021_01_07_425723 313 2 is be VBZ 10_1101-2021_01_07_425723 313 3 made make VBN 10_1101-2021_01_07_425723 313 4 The the DT 10_1101-2021_01_07_425723 313 5 copyright copyright NN 10_1101-2021_01_07_425723 313 6 holder holder NN 10_1101-2021_01_07_425723 313 7 for for IN 10_1101-2021_01_07_425723 313 8 this this DT 10_1101-2021_01_07_425723 313 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 313 10 version version NN 10_1101-2021_01_07_425723 313 11 posted post VBD 10_1101-2021_01_07_425723 313 12 January January NNP 10_1101-2021_01_07_425723 313 13 8 8 CD 10_1101-2021_01_07_425723 313 14 , , , 10_1101-2021_01_07_425723 313 15 2021 2021 CD 10_1101-2021_01_07_425723 313 16 . . . 10_1101-2021_01_07_425723 313 17 ; ; : 10_1101-2021_01_07_425723 313 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 313 19 : : : 10_1101-2021_01_07_425723 313 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 313 21 preprint preprint NN 10_1101-2021_01_07_425723 313 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 313 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 313 24 29 29 CD 10_1101-2021_01_07_425723 313 25 Rad Rad NNP 10_1101-2021_01_07_425723 313 26 HPX-87H hpx-87h NN 10_1101-2021_01_07_425723 313 27 ion ion NN 10_1101-2021_01_07_425723 313 28 exchange exchange NN 10_1101-2021_01_07_425723 313 29 column column NN 10_1101-2021_01_07_425723 313 30 ( ( -LRB- 10_1101-2021_01_07_425723 313 31 BioRad BioRad NNP 10_1101-2021_01_07_425723 313 32 , , , 10_1101-2021_01_07_425723 313 33 Veenendaal Veenendaal NNP 10_1101-2021_01_07_425723 313 34 , , , 10_1101-2021_01_07_425723 313 35 the the DT 10_1101-2021_01_07_425723 313 36 Netherlands Netherlands NNP 10_1101-2021_01_07_425723 313 37 ) ) -RRB- 10_1101-2021_01_07_425723 313 38 operated operate VBD 10_1101-2021_01_07_425723 313 39 at at IN 10_1101-2021_01_07_425723 313 40 60 60 CD 10_1101-2021_01_07_425723 313 41 ° ° NNS 10_1101-2021_01_07_425723 313 42 C C NNP 10_1101-2021_01_07_425723 313 43 with with IN 10_1101-2021_01_07_425723 313 44 a a DT 10_1101-2021_01_07_425723 313 45 474 474 CD 10_1101-2021_01_07_425723 313 46 mobile mobile JJ 10_1101-2021_01_07_425723 313 47 phase phase NN 10_1101-2021_01_07_425723 313 48 of of IN 10_1101-2021_01_07_425723 313 49 5 5 CD 10_1101-2021_01_07_425723 313 50 mM mM NNP 10_1101-2021_01_07_425723 313 51 H2SO4 H2SO4 NNP 10_1101-2021_01_07_425723 313 52 at at IN 10_1101-2021_01_07_425723 313 53 a a DT 10_1101-2021_01_07_425723 313 54 flowrate flowrate NN 10_1101-2021_01_07_425723 313 55 of of IN 10_1101-2021_01_07_425723 313 56 0.6 0.6 CD 10_1101-2021_01_07_425723 313 57 mL·min-1 mL·min-1 NNS 10_1101-2021_01_07_425723 313 58 . . . 10_1101-2021_01_07_425723 314 1 Compounds compound NNS 10_1101-2021_01_07_425723 314 2 were be VBD 10_1101-2021_01_07_425723 314 3 detected detect VBN 10_1101-2021_01_07_425723 314 4 by by IN 10_1101-2021_01_07_425723 314 5 means mean NNS 10_1101-2021_01_07_425723 314 6 of of IN 10_1101-2021_01_07_425723 314 7 a a DT 10_1101-2021_01_07_425723 314 8 475 475 CD 10_1101-2021_01_07_425723 314 9 dual dual JJ 10_1101-2021_01_07_425723 314 10 - - HYPH 10_1101-2021_01_07_425723 314 11 wavelength wavelength NN 10_1101-2021_01_07_425723 314 12 absorbance absorbance NN 10_1101-2021_01_07_425723 314 13 detector detector NN 10_1101-2021_01_07_425723 314 14 ( ( -LRB- 10_1101-2021_01_07_425723 314 15 Waters Waters NNP 10_1101-2021_01_07_425723 314 16 2487 2487 CD 10_1101-2021_01_07_425723 314 17 ) ) -RRB- 10_1101-2021_01_07_425723 314 18 and and CC 10_1101-2021_01_07_425723 314 19 a a DT 10_1101-2021_01_07_425723 314 20 refractive refractive JJ 10_1101-2021_01_07_425723 314 21 index index NN 10_1101-2021_01_07_425723 314 22 detector detector NN 10_1101-2021_01_07_425723 314 23 ( ( -LRB- 10_1101-2021_01_07_425723 314 24 Waters Waters NNP 10_1101-2021_01_07_425723 314 25 2410 2410 CD 10_1101-2021_01_07_425723 314 26 ) ) -RRB- 10_1101-2021_01_07_425723 314 27 and and CC 10_1101-2021_01_07_425723 314 28 476 476 CD 10_1101-2021_01_07_425723 314 29 compared compare VBN 10_1101-2021_01_07_425723 314 30 to to TO 10_1101-2021_01_07_425723 314 31 reference reference VB 10_1101-2021_01_07_425723 314 32 compounds compound NNS 10_1101-2021_01_07_425723 314 33 ( ( -LRB- 10_1101-2021_01_07_425723 314 34 Sigma Sigma NNP 10_1101-2021_01_07_425723 314 35 - - HYPH 10_1101-2021_01_07_425723 314 36 Aldrich Aldrich NNP 10_1101-2021_01_07_425723 314 37 ) ) -RRB- 10_1101-2021_01_07_425723 314 38 . . . 10_1101-2021_01_07_425723 315 1 Residual residual JJ 10_1101-2021_01_07_425723 315 2 glucose glucose NN 10_1101-2021_01_07_425723 315 3 concentrations concentration NNS 10_1101-2021_01_07_425723 315 4 in in IN 10_1101-2021_01_07_425723 315 5 continuous continuous JJ 10_1101-2021_01_07_425723 315 6 477 477 CD 10_1101-2021_01_07_425723 315 7 cultivations cultivation NNS 10_1101-2021_01_07_425723 315 8 were be VBD 10_1101-2021_01_07_425723 315 9 determined determine VBN 10_1101-2021_01_07_425723 315 10 by by IN 10_1101-2021_01_07_425723 315 11 HPLC hplc NN 10_1101-2021_01_07_425723 315 12 analysis analysis NN 10_1101-2021_01_07_425723 315 13 from from IN 10_1101-2021_01_07_425723 315 14 rapid rapid JJ 10_1101-2021_01_07_425723 315 15 quenched quench VBN 10_1101-2021_01_07_425723 315 16 culture culture NN 10_1101-2021_01_07_425723 315 17 samples sample NNS 10_1101-2021_01_07_425723 315 18 with with IN 10_1101-2021_01_07_425723 315 19 cold cold JJ 10_1101-2021_01_07_425723 315 20 steel steel NN 10_1101-2021_01_07_425723 315 21 478 478 CD 10_1101-2021_01_07_425723 315 22 beads67 beads67 NNS 10_1101-2021_01_07_425723 315 23 . . . 10_1101-2021_01_07_425723 316 1 479 479 CD 10_1101-2021_01_07_425723 316 2 Gas gas NN 10_1101-2021_01_07_425723 316 3 analysis analysis NN 10_1101-2021_01_07_425723 316 4 480 480 CD 10_1101-2021_01_07_425723 316 5 The the DT 10_1101-2021_01_07_425723 316 6 off off IN 10_1101-2021_01_07_425723 316 7 - - HYPH 10_1101-2021_01_07_425723 316 8 gas gas NN 10_1101-2021_01_07_425723 316 9 from from IN 10_1101-2021_01_07_425723 316 10 bioreactor bioreactor NN 10_1101-2021_01_07_425723 316 11 cultures culture NNS 10_1101-2021_01_07_425723 316 12 was be VBD 10_1101-2021_01_07_425723 316 13 cooled cool VBN 10_1101-2021_01_07_425723 316 14 with with IN 10_1101-2021_01_07_425723 316 15 a a DT 10_1101-2021_01_07_425723 316 16 condenser condenser NN 10_1101-2021_01_07_425723 316 17 ( ( -LRB- 10_1101-2021_01_07_425723 316 18 2 2 CD 10_1101-2021_01_07_425723 316 19 ° ° , 10_1101-2021_01_07_425723 316 20 C C NNP 10_1101-2021_01_07_425723 316 21 ) ) -RRB- 10_1101-2021_01_07_425723 316 22 and and CC 10_1101-2021_01_07_425723 316 23 dried dry VBN 10_1101-2021_01_07_425723 316 24 with with IN 10_1101-2021_01_07_425723 316 25 PermaPure PermaPure NNP 10_1101-2021_01_07_425723 316 26 Dryer Dryer NNP 10_1101-2021_01_07_425723 316 27 481 481 CD 10_1101-2021_01_07_425723 316 28 ( ( -LRB- 10_1101-2021_01_07_425723 316 29 Inacom Inacom NNP 10_1101-2021_01_07_425723 316 30 Instruments Instruments NNP 10_1101-2021_01_07_425723 316 31 , , , 10_1101-2021_01_07_425723 316 32 Veenendaal Veenendaal NNP 10_1101-2021_01_07_425723 316 33 , , , 10_1101-2021_01_07_425723 316 34 the the DT 10_1101-2021_01_07_425723 316 35 Netherlands Netherlands NNP 10_1101-2021_01_07_425723 316 36 ) ) -RRB- 10_1101-2021_01_07_425723 316 37 prior prior RB 10_1101-2021_01_07_425723 316 38 to to IN 10_1101-2021_01_07_425723 316 39 analysis analysis NN 10_1101-2021_01_07_425723 316 40 of of IN 10_1101-2021_01_07_425723 316 41 the the DT 10_1101-2021_01_07_425723 316 42 carbon carbon NN 10_1101-2021_01_07_425723 316 43 dioxide dioxide NN 10_1101-2021_01_07_425723 316 44 and and CC 10_1101-2021_01_07_425723 316 45 oxygen oxygen NN 10_1101-2021_01_07_425723 316 46 482 482 CD 10_1101-2021_01_07_425723 316 47 fraction fraction NN 10_1101-2021_01_07_425723 316 48 with with IN 10_1101-2021_01_07_425723 316 49 a a DT 10_1101-2021_01_07_425723 316 50 Rosemount Rosemount NNP 10_1101-2021_01_07_425723 316 51 NGA NGA NNP 10_1101-2021_01_07_425723 316 52 2000 2000 CD 10_1101-2021_01_07_425723 316 53 Analyser Analyser NNP 10_1101-2021_01_07_425723 316 54 ( ( -LRB- 10_1101-2021_01_07_425723 316 55 Baar Baar NNP 10_1101-2021_01_07_425723 316 56 , , , 10_1101-2021_01_07_425723 316 57 Switzerland Switzerland NNP 10_1101-2021_01_07_425723 316 58 ) ) -RRB- 10_1101-2021_01_07_425723 316 59 . . . 10_1101-2021_01_07_425723 317 1 The the DT 10_1101-2021_01_07_425723 317 2 Rosemount Rosemount NNP 10_1101-2021_01_07_425723 317 3 gas gas NN 10_1101-2021_01_07_425723 317 4 analyzer analyzer NN 10_1101-2021_01_07_425723 317 5 was be VBD 10_1101-2021_01_07_425723 317 6 483 483 CD 10_1101-2021_01_07_425723 317 7 calibrated calibrate VBN 10_1101-2021_01_07_425723 317 8 with with IN 10_1101-2021_01_07_425723 317 9 defined define VBN 10_1101-2021_01_07_425723 317 10 mixtures mixture NNS 10_1101-2021_01_07_425723 317 11 of of IN 10_1101-2021_01_07_425723 317 12 1.98 1.98 CD 10_1101-2021_01_07_425723 317 13 % % NN 10_1101-2021_01_07_425723 317 14 O2 o2 CD 10_1101-2021_01_07_425723 317 15 , , , 10_1101-2021_01_07_425723 317 16 3.01 3.01 CD 10_1101-2021_01_07_425723 317 17 % % NN 10_1101-2021_01_07_425723 317 18 CO2 CO2 NNP 10_1101-2021_01_07_425723 317 19 and and CC 10_1101-2021_01_07_425723 317 20 high high JJ 10_1101-2021_01_07_425723 317 21 quality quality NN 10_1101-2021_01_07_425723 317 22 nitrogen nitrogen NN 10_1101-2021_01_07_425723 317 23 gas gas NN 10_1101-2021_01_07_425723 317 24 N6 n6 NN 10_1101-2021_01_07_425723 317 25 ( ( -LRB- 10_1101-2021_01_07_425723 317 26 Linde Linde NNP 10_1101-2021_01_07_425723 317 27 AG AG NNP 10_1101-2021_01_07_425723 317 28 ) ) -RRB- 10_1101-2021_01_07_425723 317 29 . . . 10_1101-2021_01_07_425723 318 1 484 484 CD 10_1101-2021_01_07_425723 318 2 Ethanol Ethanol NNP 10_1101-2021_01_07_425723 318 3 evaporation evaporation NN 10_1101-2021_01_07_425723 318 4 rate rate NN 10_1101-2021_01_07_425723 318 5 485 485 CD 10_1101-2021_01_07_425723 318 6 To to TO 10_1101-2021_01_07_425723 318 7 correct correct VB 10_1101-2021_01_07_425723 318 8 for for IN 10_1101-2021_01_07_425723 318 9 ethanol ethanol NN 10_1101-2021_01_07_425723 318 10 evaporation evaporation NN 10_1101-2021_01_07_425723 318 11 in in IN 10_1101-2021_01_07_425723 318 12 the the DT 10_1101-2021_01_07_425723 318 13 continuous continuous JJ 10_1101-2021_01_07_425723 318 14 bioreactor bioreactor NN 10_1101-2021_01_07_425723 318 15 cultivations cultivation VBZ 10_1101-2021_01_07_425723 318 16 the the DT 10_1101-2021_01_07_425723 318 17 ethanol ethanol NN 10_1101-2021_01_07_425723 318 18 evaporation evaporation NN 10_1101-2021_01_07_425723 318 19 486 486 CD 10_1101-2021_01_07_425723 318 20 rate rate NN 10_1101-2021_01_07_425723 318 21 was be VBD 10_1101-2021_01_07_425723 318 22 determined determine VBN 10_1101-2021_01_07_425723 318 23 in in IN 10_1101-2021_01_07_425723 318 24 the the DT 10_1101-2021_01_07_425723 318 25 same same JJ 10_1101-2021_01_07_425723 318 26 experimental experimental JJ 10_1101-2021_01_07_425723 318 27 bioreactor bioreactor NN 10_1101-2021_01_07_425723 318 28 set set NN 10_1101-2021_01_07_425723 318 29 - - HYPH 10_1101-2021_01_07_425723 318 30 up up RP 10_1101-2021_01_07_425723 318 31 without without IN 10_1101-2021_01_07_425723 318 32 the the DT 10_1101-2021_01_07_425723 318 33 yeast yeast NN 10_1101-2021_01_07_425723 318 34 . . . 10_1101-2021_01_07_425723 319 1 To to IN 10_1101-2021_01_07_425723 319 2 SM SM NNP 10_1101-2021_01_07_425723 319 3 glucose glucose VB 10_1101-2021_01_07_425723 319 4 487 487 CD 10_1101-2021_01_07_425723 319 5 media medium NNS 10_1101-2021_01_07_425723 319 6 with with IN 10_1101-2021_01_07_425723 319 7 urea urea NNP 10_1101-2021_01_07_425723 319 8 400 400 CD 10_1101-2021_01_07_425723 319 9 mM mM NNP 10_1101-2021_01_07_425723 319 10 of of IN 10_1101-2021_01_07_425723 319 11 ethanol ethanol NN 10_1101-2021_01_07_425723 319 12 was be VBD 10_1101-2021_01_07_425723 319 13 added add VBN 10_1101-2021_01_07_425723 319 14 after after IN 10_1101-2021_01_07_425723 319 15 which which WDT 10_1101-2021_01_07_425723 319 16 the the DT 10_1101-2021_01_07_425723 319 17 decrease decrease NN 10_1101-2021_01_07_425723 319 18 in in IN 10_1101-2021_01_07_425723 319 19 the the DT 10_1101-2021_01_07_425723 319 20 ethanol ethanol NN 10_1101-2021_01_07_425723 319 21 concentration concentration NN 10_1101-2021_01_07_425723 319 22 488 488 CD 10_1101-2021_01_07_425723 319 23 was be VBD 10_1101-2021_01_07_425723 319 24 measured measure VBN 10_1101-2021_01_07_425723 319 25 over over IN 10_1101-2021_01_07_425723 319 26 time time NN 10_1101-2021_01_07_425723 319 27 by by IN 10_1101-2021_01_07_425723 319 28 periodic periodic JJ 10_1101-2021_01_07_425723 319 29 measurements measurement NNS 10_1101-2021_01_07_425723 319 30 and and CC 10_1101-2021_01_07_425723 319 31 quantification quantification NN 10_1101-2021_01_07_425723 319 32 by by IN 10_1101-2021_01_07_425723 319 33 HPLC hplc NN 10_1101-2021_01_07_425723 319 34 analysis analysis NN 10_1101-2021_01_07_425723 319 35 over over IN 10_1101-2021_01_07_425723 319 36 the the DT 10_1101-2021_01_07_425723 319 37 course course NN 10_1101-2021_01_07_425723 319 38 489 489 CD 10_1101-2021_01_07_425723 319 39 of of IN 10_1101-2021_01_07_425723 319 40 at at RB 10_1101-2021_01_07_425723 319 41 least least RBS 10_1101-2021_01_07_425723 319 42 140 140 CD 10_1101-2021_01_07_425723 319 43 hours hour NNS 10_1101-2021_01_07_425723 319 44 . . . 10_1101-2021_01_07_425723 320 1 To to TO 10_1101-2021_01_07_425723 320 2 reflect reflect VB 10_1101-2021_01_07_425723 320 3 the the DT 10_1101-2021_01_07_425723 320 4 media medium NNS 10_1101-2021_01_07_425723 320 5 composition composition NN 10_1101-2021_01_07_425723 320 6 used use VBN 10_1101-2021_01_07_425723 320 7 for for IN 10_1101-2021_01_07_425723 320 8 the the DT 10_1101-2021_01_07_425723 320 9 different different JJ 10_1101-2021_01_07_425723 320 10 oxygen oxygen NN 10_1101-2021_01_07_425723 320 11 regimes regime NNS 10_1101-2021_01_07_425723 320 12 and and CC 10_1101-2021_01_07_425723 320 13 490 490 CD 10_1101-2021_01_07_425723 320 14 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 320 15 growth growth NN 10_1101-2021_01_07_425723 320 16 factor factor NN 10_1101-2021_01_07_425723 320 17 supplementation supplementation NN 10_1101-2021_01_07_425723 320 18 , , , 10_1101-2021_01_07_425723 320 19 the the DT 10_1101-2021_01_07_425723 320 20 ethanol ethanol NN 10_1101-2021_01_07_425723 320 21 evaporation evaporation NN 10_1101-2021_01_07_425723 320 22 was be VBD 10_1101-2021_01_07_425723 320 23 measured measure VBN 10_1101-2021_01_07_425723 320 24 for for IN 10_1101-2021_01_07_425723 320 25 bioreactor bioreactor NN 10_1101-2021_01_07_425723 320 26 sparge sparge NN 10_1101-2021_01_07_425723 320 27 491 491 CD 10_1101-2021_01_07_425723 320 28 aeration aeration NN 10_1101-2021_01_07_425723 320 29 with with IN 10_1101-2021_01_07_425723 320 30 Tween Tween NNP 10_1101-2021_01_07_425723 320 31 80 80 CD 10_1101-2021_01_07_425723 320 32 , , , 10_1101-2021_01_07_425723 320 33 bioreactor bioreactor NN 10_1101-2021_01_07_425723 320 34 head head NN 10_1101-2021_01_07_425723 320 35 - - HYPH 10_1101-2021_01_07_425723 320 36 space space NN 10_1101-2021_01_07_425723 320 37 aeration aeration NN 10_1101-2021_01_07_425723 320 38 both both CC 10_1101-2021_01_07_425723 320 39 with with IN 10_1101-2021_01_07_425723 320 40 and and CC 10_1101-2021_01_07_425723 320 41 without without IN 10_1101-2021_01_07_425723 320 42 Tween Tween NNP 10_1101-2021_01_07_425723 320 43 80 80 CD 10_1101-2021_01_07_425723 320 44 . . . 10_1101-2021_01_07_425723 321 1 The the DT 10_1101-2021_01_07_425723 321 2 ethanol ethanol NN 10_1101-2021_01_07_425723 321 3 492 492 CD 10_1101-2021_01_07_425723 321 4 evaporation evaporation NN 10_1101-2021_01_07_425723 321 5 rate rate NN 10_1101-2021_01_07_425723 321 6 was be VBD 10_1101-2021_01_07_425723 321 7 measured measure VBN 10_1101-2021_01_07_425723 321 8 for for IN 10_1101-2021_01_07_425723 321 9 each each DT 10_1101-2021_01_07_425723 321 10 condition condition NN 10_1101-2021_01_07_425723 321 11 in in IN 10_1101-2021_01_07_425723 321 12 triplicate triplicate NN 10_1101-2021_01_07_425723 321 13 . . . 10_1101-2021_01_07_425723 322 1 493 493 CD 10_1101-2021_01_07_425723 322 2 Lipid Lipid NNP 10_1101-2021_01_07_425723 322 3 extractions extraction NNS 10_1101-2021_01_07_425723 322 4 & & CC 10_1101-2021_01_07_425723 322 5 GC GC NNP 10_1101-2021_01_07_425723 322 6 analysis analysis NN 10_1101-2021_01_07_425723 322 7 494 494 CD 10_1101-2021_01_07_425723 322 8 .CC .CC , 10_1101-2021_01_07_425723 322 9 - - HYPH 10_1101-2021_01_07_425723 322 10 BY by IN 10_1101-2021_01_07_425723 322 11 - - HYPH 10_1101-2021_01_07_425723 322 12 NC NC NNP 10_1101-2021_01_07_425723 322 13 - - HYPH 10_1101-2021_01_07_425723 322 14 ND ND NNP 10_1101-2021_01_07_425723 322 15 4.0 4.0 CD 10_1101-2021_01_07_425723 322 16 International International NNP 10_1101-2021_01_07_425723 322 17 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 322 18 under under IN 10_1101-2021_01_07_425723 322 19 a a DT 10_1101-2021_01_07_425723 322 20 ( ( -LRB- 10_1101-2021_01_07_425723 322 21 which which WDT 10_1101-2021_01_07_425723 322 22 was be VBD 10_1101-2021_01_07_425723 322 23 not not RB 10_1101-2021_01_07_425723 322 24 certified certify VBN 10_1101-2021_01_07_425723 322 25 by by IN 10_1101-2021_01_07_425723 322 26 peer peer NN 10_1101-2021_01_07_425723 322 27 review review NN 10_1101-2021_01_07_425723 322 28 ) ) -RRB- 10_1101-2021_01_07_425723 322 29 is be VBZ 10_1101-2021_01_07_425723 322 30 the the DT 10_1101-2021_01_07_425723 322 31 author author NN 10_1101-2021_01_07_425723 322 32 / / SYM 10_1101-2021_01_07_425723 322 33 funder funder NN 10_1101-2021_01_07_425723 322 34 , , , 10_1101-2021_01_07_425723 322 35 who who WP 10_1101-2021_01_07_425723 322 36 has have VBZ 10_1101-2021_01_07_425723 322 37 granted grant VBN 10_1101-2021_01_07_425723 322 38 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 322 39 a a DT 10_1101-2021_01_07_425723 322 40 license license NN 10_1101-2021_01_07_425723 322 41 to to TO 10_1101-2021_01_07_425723 322 42 display display VB 10_1101-2021_01_07_425723 322 43 the the DT 10_1101-2021_01_07_425723 322 44 preprint preprint NN 10_1101-2021_01_07_425723 322 45 in in IN 10_1101-2021_01_07_425723 322 46 perpetuity perpetuity NN 10_1101-2021_01_07_425723 322 47 . . . 10_1101-2021_01_07_425723 323 1 It -PRON- PRP 10_1101-2021_01_07_425723 323 2 is be VBZ 10_1101-2021_01_07_425723 323 3 made make VBN 10_1101-2021_01_07_425723 323 4 The the DT 10_1101-2021_01_07_425723 323 5 copyright copyright NN 10_1101-2021_01_07_425723 323 6 holder holder NN 10_1101-2021_01_07_425723 323 7 for for IN 10_1101-2021_01_07_425723 323 8 this this DT 10_1101-2021_01_07_425723 323 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 323 10 version version NN 10_1101-2021_01_07_425723 323 11 posted post VBD 10_1101-2021_01_07_425723 323 12 January January NNP 10_1101-2021_01_07_425723 323 13 8 8 CD 10_1101-2021_01_07_425723 323 14 , , , 10_1101-2021_01_07_425723 323 15 2021 2021 CD 10_1101-2021_01_07_425723 323 16 . . . 10_1101-2021_01_07_425723 323 17 ; ; : 10_1101-2021_01_07_425723 323 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 323 19 : : : 10_1101-2021_01_07_425723 323 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 323 21 preprint preprint NN 10_1101-2021_01_07_425723 323 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 323 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ RB 10_1101-2021_01_07_425723 323 24 30 30 CD 10_1101-2021_01_07_425723 323 25 For for IN 10_1101-2021_01_07_425723 323 26 analysis analysis NN 10_1101-2021_01_07_425723 323 27 of of IN 10_1101-2021_01_07_425723 323 28 triterpene triterpene JJ 10_1101-2021_01_07_425723 323 29 and and CC 10_1101-2021_01_07_425723 323 30 triterpenoid triterpenoid JJ 10_1101-2021_01_07_425723 323 31 cell cell NN 10_1101-2021_01_07_425723 323 32 contents content NNS 10_1101-2021_01_07_425723 323 33 biomass biomass NNP 10_1101-2021_01_07_425723 323 34 was be VBD 10_1101-2021_01_07_425723 323 35 harvested harvest VBN 10_1101-2021_01_07_425723 323 36 , , , 10_1101-2021_01_07_425723 323 37 washed wash VBD 10_1101-2021_01_07_425723 323 38 once once RB 10_1101-2021_01_07_425723 323 39 with with IN 10_1101-2021_01_07_425723 323 40 495 495 CD 10_1101-2021_01_07_425723 323 41 demineralized demineralized JJ 10_1101-2021_01_07_425723 323 42 water water NN 10_1101-2021_01_07_425723 323 43 and and CC 10_1101-2021_01_07_425723 323 44 stored store VBD 10_1101-2021_01_07_425723 323 45 as as IN 10_1101-2021_01_07_425723 323 46 pellet pellet NN 10_1101-2021_01_07_425723 323 47 at at IN 10_1101-2021_01_07_425723 323 48 -80 -80 NNP 10_1101-2021_01_07_425723 323 49 ° ° NNP 10_1101-2021_01_07_425723 323 50 C C NNP 10_1101-2021_01_07_425723 323 51 before before IN 10_1101-2021_01_07_425723 323 52 freeze freeze NN 10_1101-2021_01_07_425723 323 53 - - HYPH 10_1101-2021_01_07_425723 323 54 drying dry VBG 10_1101-2021_01_07_425723 323 55 the the DT 10_1101-2021_01_07_425723 323 56 pellets pellet NNS 10_1101-2021_01_07_425723 323 57 using use VBG 10_1101-2021_01_07_425723 323 58 an an DT 10_1101-2021_01_07_425723 323 59 Alpha alpha NN 10_1101-2021_01_07_425723 323 60 1 1 CD 10_1101-2021_01_07_425723 323 61 - - SYM 10_1101-2021_01_07_425723 323 62 4 4 CD 10_1101-2021_01_07_425723 323 63 LD LD NNP 10_1101-2021_01_07_425723 323 64 496 496 CD 10_1101-2021_01_07_425723 323 65 Plus plus CC 10_1101-2021_01_07_425723 323 66 ( ( -LRB- 10_1101-2021_01_07_425723 323 67 Martin Martin NNP 10_1101-2021_01_07_425723 323 68 Christ Christ NNP 10_1101-2021_01_07_425723 323 69 , , , 10_1101-2021_01_07_425723 323 70 Osterode Osterode NNP 10_1101-2021_01_07_425723 323 71 am be VBP 10_1101-2021_01_07_425723 323 72 Harz Harz NNP 10_1101-2021_01_07_425723 323 73 , , , 10_1101-2021_01_07_425723 323 74 Germany Germany NNP 10_1101-2021_01_07_425723 323 75 ) ) -RRB- 10_1101-2021_01_07_425723 323 76 at at IN 10_1101-2021_01_07_425723 323 77 -60 -60 NNP 10_1101-2021_01_07_425723 323 78 ° ° NNP 10_1101-2021_01_07_425723 323 79 C C NNP 10_1101-2021_01_07_425723 323 80 and and CC 10_1101-2021_01_07_425723 323 81 0.05 0.05 CD 10_1101-2021_01_07_425723 323 82 mbar mbar NN 10_1101-2021_01_07_425723 323 83 . . . 10_1101-2021_01_07_425723 324 1 Freeze freeze VB 10_1101-2021_01_07_425723 324 2 - - HYPH 10_1101-2021_01_07_425723 324 3 dried dry VBN 10_1101-2021_01_07_425723 324 4 biomass biomass NN 10_1101-2021_01_07_425723 324 5 was be VBD 10_1101-2021_01_07_425723 324 6 497 497 CD 10_1101-2021_01_07_425723 324 7 saponificated saponificate VBN 10_1101-2021_01_07_425723 324 8 with with IN 10_1101-2021_01_07_425723 324 9 2.0 2.0 CD 10_1101-2021_01_07_425723 324 10 M m NN 10_1101-2021_01_07_425723 324 11 NaOH naoh NN 10_1101-2021_01_07_425723 324 12 ( ( -LRB- 10_1101-2021_01_07_425723 324 13 Bio Bio NNP 10_1101-2021_01_07_425723 324 14 - - HYPH 10_1101-2021_01_07_425723 324 15 Ultra Ultra NNP 10_1101-2021_01_07_425723 324 16 , , , 10_1101-2021_01_07_425723 324 17 Sigma Sigma NNP 10_1101-2021_01_07_425723 324 18 - - HYPH 10_1101-2021_01_07_425723 324 19 Aldrich Aldrich NNP 10_1101-2021_01_07_425723 324 20 ) ) -RRB- 10_1101-2021_01_07_425723 324 21 in in IN 10_1101-2021_01_07_425723 324 22 methylation methylation NN 10_1101-2021_01_07_425723 324 23 glass glass NN 10_1101-2021_01_07_425723 324 24 tubes tube NNS 10_1101-2021_01_07_425723 324 25 ( ( -LRB- 10_1101-2021_01_07_425723 324 26 PYREXTM PYREXTM NNP 10_1101-2021_01_07_425723 324 27 498 498 CD 10_1101-2021_01_07_425723 324 28 Boroslicate Boroslicate NNP 10_1101-2021_01_07_425723 324 29 glass glass NN 10_1101-2021_01_07_425723 324 30 , , , 10_1101-2021_01_07_425723 324 31 Thermo Thermo NNP 10_1101-2021_01_07_425723 324 32 Fisher Fisher NNP 10_1101-2021_01_07_425723 324 33 Scientific Scientific NNP 10_1101-2021_01_07_425723 324 34 ) ) -RRB- 10_1101-2021_01_07_425723 324 35 at at IN 10_1101-2021_01_07_425723 324 36 70 70 CD 10_1101-2021_01_07_425723 324 37 ° ° , 10_1101-2021_01_07_425723 324 38 C C NNP 10_1101-2021_01_07_425723 324 39 . . . 10_1101-2021_01_07_425723 325 1 As as IN 10_1101-2021_01_07_425723 325 2 internal internal JJ 10_1101-2021_01_07_425723 325 3 standard standard JJ 10_1101-2021_01_07_425723 325 4 5α 5α NNP 10_1101-2021_01_07_425723 325 5 - - HYPH 10_1101-2021_01_07_425723 325 6 cholestane cholestane NNP 10_1101-2021_01_07_425723 325 7 ( ( -LRB- 10_1101-2021_01_07_425723 325 8 Sigma Sigma NNP 10_1101-2021_01_07_425723 325 9 - - HYPH 10_1101-2021_01_07_425723 325 10 Aldrich Aldrich NNP 10_1101-2021_01_07_425723 325 11 ) ) -RRB- 10_1101-2021_01_07_425723 325 12 499 499 CD 10_1101-2021_01_07_425723 325 13 was be VBD 10_1101-2021_01_07_425723 325 14 added add VBN 10_1101-2021_01_07_425723 325 15 to to IN 10_1101-2021_01_07_425723 325 16 the the DT 10_1101-2021_01_07_425723 325 17 saponified saponified JJ 10_1101-2021_01_07_425723 325 18 biomass biomass NN 10_1101-2021_01_07_425723 325 19 suspension suspension NN 10_1101-2021_01_07_425723 325 20 . . . 10_1101-2021_01_07_425723 326 1 Subsequently subsequently RB 10_1101-2021_01_07_425723 326 2 tert tert NN 10_1101-2021_01_07_425723 326 3 - - HYPH 10_1101-2021_01_07_425723 326 4 butyl butyl NN 10_1101-2021_01_07_425723 326 5 - - HYPH 10_1101-2021_01_07_425723 326 6 methyl methyl NN 10_1101-2021_01_07_425723 326 7 - - HYPH 10_1101-2021_01_07_425723 326 8 ether ether NN 10_1101-2021_01_07_425723 326 9 ( ( -LRB- 10_1101-2021_01_07_425723 326 10 tBME tbme NN 10_1101-2021_01_07_425723 326 11 , , , 10_1101-2021_01_07_425723 326 12 Sigma-500 Sigma-500 . 10_1101-2021_01_07_425723 326 13 Aldrich Aldrich NNP 10_1101-2021_01_07_425723 326 14 ) ) -RRB- 10_1101-2021_01_07_425723 326 15 was be VBD 10_1101-2021_01_07_425723 326 16 added add VBN 10_1101-2021_01_07_425723 326 17 for for IN 10_1101-2021_01_07_425723 326 18 organic organic JJ 10_1101-2021_01_07_425723 326 19 phase phase NN 10_1101-2021_01_07_425723 326 20 extraction extraction NN 10_1101-2021_01_07_425723 326 21 . . . 10_1101-2021_01_07_425723 327 1 Samples sample NNS 10_1101-2021_01_07_425723 327 2 were be VBD 10_1101-2021_01_07_425723 327 3 extracted extract VBN 10_1101-2021_01_07_425723 327 4 twice twice RB 10_1101-2021_01_07_425723 327 5 using use VBG 10_1101-2021_01_07_425723 327 6 tBME tbme NN 10_1101-2021_01_07_425723 327 7 and and CC 10_1101-2021_01_07_425723 327 8 dried dry VBD 10_1101-2021_01_07_425723 327 9 501 501 CD 10_1101-2021_01_07_425723 327 10 with with IN 10_1101-2021_01_07_425723 327 11 sodium sodium NN 10_1101-2021_01_07_425723 327 12 - - HYPH 10_1101-2021_01_07_425723 327 13 sulfate sulfate NN 10_1101-2021_01_07_425723 327 14 ( ( -LRB- 10_1101-2021_01_07_425723 327 15 Merck Merck NNP 10_1101-2021_01_07_425723 327 16 , , , 10_1101-2021_01_07_425723 327 17 Darmstadt Darmstadt NNP 10_1101-2021_01_07_425723 327 18 , , , 10_1101-2021_01_07_425723 327 19 Germany Germany NNP 10_1101-2021_01_07_425723 327 20 ) ) -RRB- 10_1101-2021_01_07_425723 327 21 to to TO 10_1101-2021_01_07_425723 327 22 remove remove VB 10_1101-2021_01_07_425723 327 23 remaining remain VBG 10_1101-2021_01_07_425723 327 24 traces trace NNS 10_1101-2021_01_07_425723 327 25 of of IN 10_1101-2021_01_07_425723 327 26 water water NN 10_1101-2021_01_07_425723 327 27 . . . 10_1101-2021_01_07_425723 328 1 The the DT 10_1101-2021_01_07_425723 328 2 organic organic JJ 10_1101-2021_01_07_425723 328 3 502 502 CD 10_1101-2021_01_07_425723 328 4 phase phase NN 10_1101-2021_01_07_425723 328 5 was be VBD 10_1101-2021_01_07_425723 328 6 either either CC 10_1101-2021_01_07_425723 328 7 concentrated concentrate VBN 10_1101-2021_01_07_425723 328 8 by by IN 10_1101-2021_01_07_425723 328 9 evaporation evaporation NN 10_1101-2021_01_07_425723 328 10 with with IN 10_1101-2021_01_07_425723 328 11 N2 N2 NNP 10_1101-2021_01_07_425723 328 12 gas gas NN 10_1101-2021_01_07_425723 328 13 aeration aeration NN 10_1101-2021_01_07_425723 328 14 or or CC 10_1101-2021_01_07_425723 328 15 transferred transfer VBD 10_1101-2021_01_07_425723 328 16 directly directly RB 10_1101-2021_01_07_425723 328 17 to to IN 10_1101-2021_01_07_425723 328 18 an an DT 10_1101-2021_01_07_425723 328 19 503 503 CD 10_1101-2021_01_07_425723 328 20 injection injection NN 10_1101-2021_01_07_425723 328 21 vial vial NN 10_1101-2021_01_07_425723 328 22 ( ( -LRB- 10_1101-2021_01_07_425723 328 23 VWR VWR NNP 10_1101-2021_01_07_425723 328 24 International International NNP 10_1101-2021_01_07_425723 328 25 , , , 10_1101-2021_01_07_425723 328 26 Amsterdam Amsterdam NNP 10_1101-2021_01_07_425723 328 27 , , , 10_1101-2021_01_07_425723 328 28 the the DT 10_1101-2021_01_07_425723 328 29 Netherlands Netherlands NNP 10_1101-2021_01_07_425723 328 30 ) ) -RRB- 10_1101-2021_01_07_425723 328 31 . . . 10_1101-2021_01_07_425723 329 1 The the DT 10_1101-2021_01_07_425723 329 2 contents content NNS 10_1101-2021_01_07_425723 329 3 were be VBD 10_1101-2021_01_07_425723 329 4 measured measure VBN 10_1101-2021_01_07_425723 329 5 by by IN 10_1101-2021_01_07_425723 329 6 GC GC NNP 10_1101-2021_01_07_425723 329 7 - - HYPH 10_1101-2021_01_07_425723 329 8 FID FID NNP 10_1101-2021_01_07_425723 329 9 504 504 CD 10_1101-2021_01_07_425723 329 10 using use VBG 10_1101-2021_01_07_425723 329 11 Agilent Agilent NNP 10_1101-2021_01_07_425723 329 12 7890A 7890A NNP 10_1101-2021_01_07_425723 329 13 Gas Gas NNP 10_1101-2021_01_07_425723 329 14 Chromatograph Chromatograph NNP 10_1101-2021_01_07_425723 329 15 ( ( -LRB- 10_1101-2021_01_07_425723 329 16 Agilent Agilent NNP 10_1101-2021_01_07_425723 329 17 Technologies Technologies NNP 10_1101-2021_01_07_425723 329 18 , , , 10_1101-2021_01_07_425723 329 19 Santa Santa NNP 10_1101-2021_01_07_425723 329 20 Clara Clara NNP 10_1101-2021_01_07_425723 329 21 , , , 10_1101-2021_01_07_425723 329 22 CA CA NNP 10_1101-2021_01_07_425723 329 23 ) ) -RRB- 10_1101-2021_01_07_425723 329 24 equipped equip VBD 10_1101-2021_01_07_425723 329 25 with with IN 10_1101-2021_01_07_425723 329 26 an an DT 10_1101-2021_01_07_425723 329 27 505 505 CD 10_1101-2021_01_07_425723 329 28 Agilent Agilent NNP 10_1101-2021_01_07_425723 329 29 CP9013 cp9013 NN 10_1101-2021_01_07_425723 329 30 column column NN 10_1101-2021_01_07_425723 329 31 ( ( -LRB- 10_1101-2021_01_07_425723 329 32 Agilent Agilent NNP 10_1101-2021_01_07_425723 329 33 ) ) -RRB- 10_1101-2021_01_07_425723 329 34 . . . 10_1101-2021_01_07_425723 330 1 The the DT 10_1101-2021_01_07_425723 330 2 oven oven NN 10_1101-2021_01_07_425723 330 3 was be VBD 10_1101-2021_01_07_425723 330 4 programmed program VBN 10_1101-2021_01_07_425723 330 5 to to TO 10_1101-2021_01_07_425723 330 6 start start VB 10_1101-2021_01_07_425723 330 7 at at IN 10_1101-2021_01_07_425723 330 8 80 80 CD 10_1101-2021_01_07_425723 330 9 ° ° , 10_1101-2021_01_07_425723 330 10 C C NNP 10_1101-2021_01_07_425723 330 11 for for IN 10_1101-2021_01_07_425723 330 12 1 1 CD 10_1101-2021_01_07_425723 330 13 min min NNP 10_1101-2021_01_07_425723 330 14 , , , 10_1101-2021_01_07_425723 330 15 ramp ramp NNP 10_1101-2021_01_07_425723 330 16 first first RB 10_1101-2021_01_07_425723 330 17 to to IN 10_1101-2021_01_07_425723 330 18 280 280 CD 10_1101-2021_01_07_425723 330 19 506 506 CD 10_1101-2021_01_07_425723 330 20 ° ° , 10_1101-2021_01_07_425723 330 21 C C NNP 10_1101-2021_01_07_425723 330 22 with with IN 10_1101-2021_01_07_425723 330 23 60 60 CD 10_1101-2021_01_07_425723 330 24 ° ° NNS 10_1101-2021_01_07_425723 330 25 C·min-1 C·min-1 NNP 10_1101-2021_01_07_425723 330 26 and and CC 10_1101-2021_01_07_425723 330 27 secondly secondly RB 10_1101-2021_01_07_425723 330 28 to to IN 10_1101-2021_01_07_425723 330 29 320 320 CD 10_1101-2021_01_07_425723 330 30 ° ° , 10_1101-2021_01_07_425723 330 31 C C NNP 10_1101-2021_01_07_425723 330 32 with with IN 10_1101-2021_01_07_425723 330 33 a a DT 10_1101-2021_01_07_425723 330 34 rate rate NN 10_1101-2021_01_07_425723 330 35 of of IN 10_1101-2021_01_07_425723 330 36 10 10 CD 10_1101-2021_01_07_425723 330 37 ° ° , 10_1101-2021_01_07_425723 330 38 C·min-1 C·min-1 NNP 10_1101-2021_01_07_425723 330 39 with with IN 10_1101-2021_01_07_425723 330 40 a a DT 10_1101-2021_01_07_425723 330 41 final final JJ 10_1101-2021_01_07_425723 330 42 temperature temperature NN 10_1101-2021_01_07_425723 330 43 hold hold NN 10_1101-2021_01_07_425723 330 44 of of IN 10_1101-2021_01_07_425723 330 45 15 15 CD 10_1101-2021_01_07_425723 330 46 507 507 CD 10_1101-2021_01_07_425723 330 47 min min NN 10_1101-2021_01_07_425723 330 48 . . . 10_1101-2021_01_07_425723 331 1 Spectra Spectra NNP 10_1101-2021_01_07_425723 331 2 were be VBD 10_1101-2021_01_07_425723 331 3 compared compare VBN 10_1101-2021_01_07_425723 331 4 to to IN 10_1101-2021_01_07_425723 331 5 separate separate JJ 10_1101-2021_01_07_425723 331 6 calibration calibration NN 10_1101-2021_01_07_425723 331 7 lines line NNS 10_1101-2021_01_07_425723 331 8 of of IN 10_1101-2021_01_07_425723 331 9 squalene squalene NN 10_1101-2021_01_07_425723 331 10 , , , 10_1101-2021_01_07_425723 331 11 ergosterol ergosterol UH 10_1101-2021_01_07_425723 331 12 , , , 10_1101-2021_01_07_425723 331 13 α α NNP 10_1101-2021_01_07_425723 331 14 - - HYPH 10_1101-2021_01_07_425723 331 15 cholestane cholestane NNP 10_1101-2021_01_07_425723 331 16 , , , 10_1101-2021_01_07_425723 331 17 508 508 CD 10_1101-2021_01_07_425723 331 18 cholesterol cholesterol NN 10_1101-2021_01_07_425723 331 19 and and CC 10_1101-2021_01_07_425723 331 20 tetrahymanol tetrahymanol NNS 10_1101-2021_01_07_425723 331 21 as as IN 10_1101-2021_01_07_425723 331 22 described describe VBN 10_1101-2021_01_07_425723 331 23 previously46 previously46 CD 10_1101-2021_01_07_425723 331 24 . . . 10_1101-2021_01_07_425723 332 1 509 509 CD 10_1101-2021_01_07_425723 332 2 Sterol Sterol NNP 10_1101-2021_01_07_425723 332 3 uptake uptake NN 10_1101-2021_01_07_425723 332 4 assay assay NN 10_1101-2021_01_07_425723 332 5 510 510 CD 10_1101-2021_01_07_425723 332 6 Sterol Sterol NNP 10_1101-2021_01_07_425723 332 7 uptake uptake NN 10_1101-2021_01_07_425723 332 8 was be VBD 10_1101-2021_01_07_425723 332 9 monitored monitor VBN 10_1101-2021_01_07_425723 332 10 by by IN 10_1101-2021_01_07_425723 332 11 the the DT 10_1101-2021_01_07_425723 332 12 uptake uptake NN 10_1101-2021_01_07_425723 332 13 of of IN 10_1101-2021_01_07_425723 332 14 fluorescently fluorescently RB 10_1101-2021_01_07_425723 332 15 labelled label VBN 10_1101-2021_01_07_425723 332 16 25-NBD 25-nbd CD 10_1101-2021_01_07_425723 332 17 - - HYPH 10_1101-2021_01_07_425723 332 18 cholesterol cholesterol NN 10_1101-2021_01_07_425723 332 19 ( ( -LRB- 10_1101-2021_01_07_425723 332 20 Avanti Avanti NNP 10_1101-2021_01_07_425723 332 21 Polar Polar NNP 10_1101-2021_01_07_425723 332 22 511 511 CD 10_1101-2021_01_07_425723 332 23 Lipids Lipids NNPS 10_1101-2021_01_07_425723 332 24 , , , 10_1101-2021_01_07_425723 332 25 Alabaster Alabaster NNP 10_1101-2021_01_07_425723 332 26 , , , 10_1101-2021_01_07_425723 332 27 AL AL NNP 10_1101-2021_01_07_425723 332 28 ) ) -RRB- 10_1101-2021_01_07_425723 332 29 . . . 10_1101-2021_01_07_425723 333 1 A a DT 10_1101-2021_01_07_425723 333 2 stock stock NN 10_1101-2021_01_07_425723 333 3 solution solution NN 10_1101-2021_01_07_425723 333 4 of of IN 10_1101-2021_01_07_425723 333 5 25-NBD 25-nbd CD 10_1101-2021_01_07_425723 333 6 - - HYPH 10_1101-2021_01_07_425723 333 7 cholesterol cholesterol NN 10_1101-2021_01_07_425723 333 8 ( ( -LRB- 10_1101-2021_01_07_425723 333 9 NBDC NBDC NNP 10_1101-2021_01_07_425723 333 10 ) ) -RRB- 10_1101-2021_01_07_425723 333 11 was be VBD 10_1101-2021_01_07_425723 333 12 prepared prepare VBN 10_1101-2021_01_07_425723 333 13 in in IN 10_1101-2021_01_07_425723 333 14 ethanol ethanol NN 10_1101-2021_01_07_425723 333 15 under under IN 10_1101-2021_01_07_425723 333 16 an an DT 10_1101-2021_01_07_425723 333 17 512 512 CD 10_1101-2021_01_07_425723 333 18 argon argon NN 10_1101-2021_01_07_425723 333 19 atmosphere atmosphere NN 10_1101-2021_01_07_425723 333 20 and and CC 10_1101-2021_01_07_425723 333 21 stored store VBD 10_1101-2021_01_07_425723 333 22 at at IN 10_1101-2021_01_07_425723 333 23 -20 -20 NNP 10_1101-2021_01_07_425723 333 24 ° ° NNP 10_1101-2021_01_07_425723 333 25 C C NNP 10_1101-2021_01_07_425723 333 26 . . . 10_1101-2021_01_07_425723 334 1 Shake shake VB 10_1101-2021_01_07_425723 334 2 flasks flask NNS 10_1101-2021_01_07_425723 334 3 with with IN 10_1101-2021_01_07_425723 334 4 10 10 CD 10_1101-2021_01_07_425723 334 5 mL mL NNP 10_1101-2021_01_07_425723 334 6 SM SM NNP 10_1101-2021_01_07_425723 334 7 glucose glucose NN 10_1101-2021_01_07_425723 334 8 media medium NNS 10_1101-2021_01_07_425723 334 9 were be VBD 10_1101-2021_01_07_425723 334 10 inoculated inoculate VBN 10_1101-2021_01_07_425723 334 11 with with IN 10_1101-2021_01_07_425723 334 12 513 513 CD 10_1101-2021_01_07_425723 334 13 yeast yeast NN 10_1101-2021_01_07_425723 334 14 strains strain NNS 10_1101-2021_01_07_425723 334 15 from from IN 10_1101-2021_01_07_425723 334 16 a a DT 10_1101-2021_01_07_425723 334 17 cryo cryo NN 10_1101-2021_01_07_425723 334 18 - - HYPH 10_1101-2021_01_07_425723 334 19 stock stock NN 10_1101-2021_01_07_425723 334 20 and and CC 10_1101-2021_01_07_425723 334 21 cultivated cultivate VBN 10_1101-2021_01_07_425723 334 22 aerobically aerobically RB 10_1101-2021_01_07_425723 334 23 at at IN 10_1101-2021_01_07_425723 334 24 200 200 CD 10_1101-2021_01_07_425723 334 25 rpm rpm NN 10_1101-2021_01_07_425723 334 26 at at IN 10_1101-2021_01_07_425723 334 27 30 30 CD 10_1101-2021_01_07_425723 334 28 ° ° , 10_1101-2021_01_07_425723 334 29 C C NNP 10_1101-2021_01_07_425723 334 30 overnight overnight RB 10_1101-2021_01_07_425723 334 31 . . . 10_1101-2021_01_07_425723 335 1 The the DT 10_1101-2021_01_07_425723 335 2 yeast yeast NN 10_1101-2021_01_07_425723 335 3 514 514 CD 10_1101-2021_01_07_425723 335 4 cultures culture NNS 10_1101-2021_01_07_425723 335 5 were be VBD 10_1101-2021_01_07_425723 335 6 subsequently subsequently RB 10_1101-2021_01_07_425723 335 7 diluted dilute VBN 10_1101-2021_01_07_425723 335 8 to to IN 10_1101-2021_01_07_425723 335 9 an an DT 10_1101-2021_01_07_425723 335 10 OD660 od660 NN 10_1101-2021_01_07_425723 335 11 of of IN 10_1101-2021_01_07_425723 335 12 0.2 0.2 CD 10_1101-2021_01_07_425723 335 13 in in IN 10_1101-2021_01_07_425723 335 14 400 400 CD 10_1101-2021_01_07_425723 335 15 mL mL NNP 10_1101-2021_01_07_425723 335 16 SM SM NNP 10_1101-2021_01_07_425723 335 17 glucose glucose NN 10_1101-2021_01_07_425723 335 18 media medium NNS 10_1101-2021_01_07_425723 335 19 in in IN 10_1101-2021_01_07_425723 335 20 500 500 CD 10_1101-2021_01_07_425723 335 21 mL mL NNP 10_1101-2021_01_07_425723 335 22 shake shake VBP 10_1101-2021_01_07_425723 335 23 515 515 CD 10_1101-2021_01_07_425723 335 24 flasks flask NNS 10_1101-2021_01_07_425723 335 25 to to TO 10_1101-2021_01_07_425723 335 26 gradually gradually RB 10_1101-2021_01_07_425723 335 27 reduce reduce VB 10_1101-2021_01_07_425723 335 28 the the DT 10_1101-2021_01_07_425723 335 29 availability availability NN 10_1101-2021_01_07_425723 335 30 of of IN 10_1101-2021_01_07_425723 335 31 oxygen oxygen NN 10_1101-2021_01_07_425723 335 32 and and CC 10_1101-2021_01_07_425723 335 33 incubated incubate VBD 10_1101-2021_01_07_425723 335 34 overnight overnight RB 10_1101-2021_01_07_425723 335 35 . . . 10_1101-2021_01_07_425723 336 1 Yeast yeast NN 10_1101-2021_01_07_425723 336 2 cultures culture NNS 10_1101-2021_01_07_425723 336 3 were be VBD 10_1101-2021_01_07_425723 336 4 516 516 CD 10_1101-2021_01_07_425723 336 5 transferred transfer VBN 10_1101-2021_01_07_425723 336 6 to to IN 10_1101-2021_01_07_425723 336 7 fresh fresh JJ 10_1101-2021_01_07_425723 336 8 SM SM NNP 10_1101-2021_01_07_425723 336 9 media medium NNS 10_1101-2021_01_07_425723 336 10 with with IN 10_1101-2021_01_07_425723 336 11 40 40 CD 10_1101-2021_01_07_425723 336 12 g·L-1 g·l-1 CD 10_1101-2021_01_07_425723 336 13 glucose glucose NN 10_1101-2021_01_07_425723 336 14 and and CC 10_1101-2021_01_07_425723 336 15 incubated incubate VBD 10_1101-2021_01_07_425723 336 16 under under IN 10_1101-2021_01_07_425723 336 17 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 336 18 conditions condition NNS 10_1101-2021_01_07_425723 336 19 at at IN 10_1101-2021_01_07_425723 336 20 30 30 CD 10_1101-2021_01_07_425723 336 21 ° ° , 10_1101-2021_01_07_425723 336 22 C C NNP 10_1101-2021_01_07_425723 336 23 517 517 CD 10_1101-2021_01_07_425723 336 24 .CC .CC : 10_1101-2021_01_07_425723 336 25 - - HYPH 10_1101-2021_01_07_425723 336 26 BY by IN 10_1101-2021_01_07_425723 336 27 - - HYPH 10_1101-2021_01_07_425723 336 28 NC NC NNP 10_1101-2021_01_07_425723 336 29 - - HYPH 10_1101-2021_01_07_425723 336 30 ND ND NNP 10_1101-2021_01_07_425723 336 31 4.0 4.0 CD 10_1101-2021_01_07_425723 336 32 International International NNP 10_1101-2021_01_07_425723 336 33 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 336 34 under under IN 10_1101-2021_01_07_425723 336 35 a a DT 10_1101-2021_01_07_425723 336 36 ( ( -LRB- 10_1101-2021_01_07_425723 336 37 which which WDT 10_1101-2021_01_07_425723 336 38 was be VBD 10_1101-2021_01_07_425723 336 39 not not RB 10_1101-2021_01_07_425723 336 40 certified certify VBN 10_1101-2021_01_07_425723 336 41 by by IN 10_1101-2021_01_07_425723 336 42 peer peer NN 10_1101-2021_01_07_425723 336 43 review review NN 10_1101-2021_01_07_425723 336 44 ) ) -RRB- 10_1101-2021_01_07_425723 336 45 is be VBZ 10_1101-2021_01_07_425723 336 46 the the DT 10_1101-2021_01_07_425723 336 47 author author NN 10_1101-2021_01_07_425723 336 48 / / SYM 10_1101-2021_01_07_425723 336 49 funder funder NN 10_1101-2021_01_07_425723 336 50 , , , 10_1101-2021_01_07_425723 336 51 who who WP 10_1101-2021_01_07_425723 336 52 has have VBZ 10_1101-2021_01_07_425723 336 53 granted grant VBN 10_1101-2021_01_07_425723 336 54 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 336 55 a a DT 10_1101-2021_01_07_425723 336 56 license license NN 10_1101-2021_01_07_425723 336 57 to to TO 10_1101-2021_01_07_425723 336 58 display display VB 10_1101-2021_01_07_425723 336 59 the the DT 10_1101-2021_01_07_425723 336 60 preprint preprint NN 10_1101-2021_01_07_425723 336 61 in in IN 10_1101-2021_01_07_425723 336 62 perpetuity perpetuity NN 10_1101-2021_01_07_425723 336 63 . . . 10_1101-2021_01_07_425723 337 1 It -PRON- PRP 10_1101-2021_01_07_425723 337 2 is be VBZ 10_1101-2021_01_07_425723 337 3 made make VBN 10_1101-2021_01_07_425723 337 4 The the DT 10_1101-2021_01_07_425723 337 5 copyright copyright NN 10_1101-2021_01_07_425723 337 6 holder holder NN 10_1101-2021_01_07_425723 337 7 for for IN 10_1101-2021_01_07_425723 337 8 this this DT 10_1101-2021_01_07_425723 337 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 337 10 version version NN 10_1101-2021_01_07_425723 337 11 posted post VBD 10_1101-2021_01_07_425723 337 12 January January NNP 10_1101-2021_01_07_425723 337 13 8 8 CD 10_1101-2021_01_07_425723 337 14 , , , 10_1101-2021_01_07_425723 337 15 2021 2021 CD 10_1101-2021_01_07_425723 337 16 . . . 10_1101-2021_01_07_425723 337 17 ; ; : 10_1101-2021_01_07_425723 337 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 337 19 : : : 10_1101-2021_01_07_425723 337 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 337 21 preprint preprint NN 10_1101-2021_01_07_425723 337 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 337 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425723 337 24 31 31 CD 10_1101-2021_01_07_425723 337 25 at at IN 10_1101-2021_01_07_425723 337 26 200 200 CD 10_1101-2021_01_07_425723 337 27 rpm rpm NN 10_1101-2021_01_07_425723 337 28 . . . 10_1101-2021_01_07_425723 338 1 After after IN 10_1101-2021_01_07_425723 338 2 22 22 CD 10_1101-2021_01_07_425723 338 3 hours hour NNS 10_1101-2021_01_07_425723 338 4 of of IN 10_1101-2021_01_07_425723 338 5 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 338 6 incubation incubation NN 10_1101-2021_01_07_425723 338 7 4 4 CD 10_1101-2021_01_07_425723 338 8 µg·L-1 µg·L-1 NNP 10_1101-2021_01_07_425723 338 9 NBD NBD NNP 10_1101-2021_01_07_425723 338 10 - - HYPH 10_1101-2021_01_07_425723 338 11 cholesterol cholesterol NN 10_1101-2021_01_07_425723 338 12 with with IN 10_1101-2021_01_07_425723 338 13 420 420 CD 10_1101-2021_01_07_425723 338 14 mg·L-1 mg·l-1 NN 10_1101-2021_01_07_425723 338 15 Tween Tween NNP 10_1101-2021_01_07_425723 338 16 80 80 CD 10_1101-2021_01_07_425723 338 17 518 518 CD 10_1101-2021_01_07_425723 338 18 were be VBD 10_1101-2021_01_07_425723 338 19 pulsed pulse VBN 10_1101-2021_01_07_425723 338 20 to to IN 10_1101-2021_01_07_425723 338 21 the the DT 10_1101-2021_01_07_425723 338 22 cultures culture NNS 10_1101-2021_01_07_425723 338 23 . . . 10_1101-2021_01_07_425723 339 1 Samples sample NNS 10_1101-2021_01_07_425723 339 2 were be VBD 10_1101-2021_01_07_425723 339 3 taken take VBN 10_1101-2021_01_07_425723 339 4 and and CC 10_1101-2021_01_07_425723 339 5 washed wash VBN 10_1101-2021_01_07_425723 339 6 with with IN 10_1101-2021_01_07_425723 339 7 PBS PBS NNP 10_1101-2021_01_07_425723 339 8 5 5 CD 10_1101-2021_01_07_425723 339 9 mL·L-1 mL·L-1 NNP 10_1101-2021_01_07_425723 339 10 Tergitol Tergitol NNP 10_1101-2021_01_07_425723 339 11 NP-40 NP-40 NNP 10_1101-2021_01_07_425723 339 12 pH pH NNP 10_1101-2021_01_07_425723 339 13 7.0 7.0 CD 10_1101-2021_01_07_425723 339 14 519 519 CD 10_1101-2021_01_07_425723 339 15 ( ( -LRB- 10_1101-2021_01_07_425723 339 16 Sigma Sigma NNP 10_1101-2021_01_07_425723 339 17 - - HYPH 10_1101-2021_01_07_425723 339 18 Aldrich Aldrich NNP 10_1101-2021_01_07_425723 339 19 ) ) -RRB- 10_1101-2021_01_07_425723 339 20 twice twice RB 10_1101-2021_01_07_425723 339 21 before before IN 10_1101-2021_01_07_425723 339 22 resuspension resuspension NN 10_1101-2021_01_07_425723 339 23 in in IN 10_1101-2021_01_07_425723 339 24 PBS PBS NNP 10_1101-2021_01_07_425723 339 25 and and CC 10_1101-2021_01_07_425723 339 26 subsequent subsequent JJ 10_1101-2021_01_07_425723 339 27 analysis analysis NN 10_1101-2021_01_07_425723 339 28 . . . 10_1101-2021_01_07_425723 340 1 Propidium Propidium NNP 10_1101-2021_01_07_425723 340 2 Iodide Iodide NNP 10_1101-2021_01_07_425723 340 3 ( ( -LRB- 10_1101-2021_01_07_425723 340 4 PI PI NNP 10_1101-2021_01_07_425723 340 5 ) ) -RRB- 10_1101-2021_01_07_425723 340 6 520 520 CD 10_1101-2021_01_07_425723 340 7 ( ( -LRB- 10_1101-2021_01_07_425723 340 8 Invitrogen Invitrogen NNP 10_1101-2021_01_07_425723 340 9 ) ) -RRB- 10_1101-2021_01_07_425723 340 10 was be VBD 10_1101-2021_01_07_425723 340 11 added add VBN 10_1101-2021_01_07_425723 340 12 to to IN 10_1101-2021_01_07_425723 340 13 the the DT 10_1101-2021_01_07_425723 340 14 sample sample NN 10_1101-2021_01_07_425723 340 15 ( ( -LRB- 10_1101-2021_01_07_425723 340 16 20 20 CD 10_1101-2021_01_07_425723 340 17 µM µM NNP 10_1101-2021_01_07_425723 340 18 ) ) -RRB- 10_1101-2021_01_07_425723 340 19 and and CC 10_1101-2021_01_07_425723 340 20 stained stain VBN 10_1101-2021_01_07_425723 340 21 according accord VBG 10_1101-2021_01_07_425723 340 22 to to IN 10_1101-2021_01_07_425723 340 23 the the DT 10_1101-2021_01_07_425723 340 24 manufacturer manufacturer NN 10_1101-2021_01_07_425723 340 25 ’s ’s POS 10_1101-2021_01_07_425723 340 26 521 521 CD 10_1101-2021_01_07_425723 340 27 instructions68 instructions68 NNS 10_1101-2021_01_07_425723 340 28 . . . 10_1101-2021_01_07_425723 341 1 PI pi NN 10_1101-2021_01_07_425723 341 2 intercalates intercalate NNS 10_1101-2021_01_07_425723 341 3 with with IN 10_1101-2021_01_07_425723 341 4 DNA dna NN 10_1101-2021_01_07_425723 341 5 in in IN 10_1101-2021_01_07_425723 341 6 cells cell NNS 10_1101-2021_01_07_425723 341 7 with with IN 10_1101-2021_01_07_425723 341 8 a a DT 10_1101-2021_01_07_425723 341 9 compromised compromised JJ 10_1101-2021_01_07_425723 341 10 cell cell NN 10_1101-2021_01_07_425723 341 11 membrane membrane NN 10_1101-2021_01_07_425723 341 12 , , , 10_1101-2021_01_07_425723 341 13 which which WDT 10_1101-2021_01_07_425723 341 14 results result VBZ 10_1101-2021_01_07_425723 341 15 in in IN 10_1101-2021_01_07_425723 341 16 red red JJ 10_1101-2021_01_07_425723 341 17 522 522 CD 10_1101-2021_01_07_425723 341 18 fluorescence fluorescence NN 10_1101-2021_01_07_425723 341 19 . . . 10_1101-2021_01_07_425723 342 1 Samples sample NNS 10_1101-2021_01_07_425723 342 2 both both CC 10_1101-2021_01_07_425723 342 3 unstained unstained JJ 10_1101-2021_01_07_425723 342 4 and and CC 10_1101-2021_01_07_425723 342 5 stained stain VBN 10_1101-2021_01_07_425723 342 6 with with IN 10_1101-2021_01_07_425723 342 7 PI PI NNP 10_1101-2021_01_07_425723 342 8 were be VBD 10_1101-2021_01_07_425723 342 9 analyzed analyze VBN 10_1101-2021_01_07_425723 342 10 with with IN 10_1101-2021_01_07_425723 342 11 Accuri Accuri NNP 10_1101-2021_01_07_425723 342 12 C6 C6 NNP 10_1101-2021_01_07_425723 342 13 flow flow NN 10_1101-2021_01_07_425723 342 14 cytometer cytometer NN 10_1101-2021_01_07_425723 342 15 523 523 CD 10_1101-2021_01_07_425723 342 16 ( ( -LRB- 10_1101-2021_01_07_425723 342 17 BD BD NNP 10_1101-2021_01_07_425723 342 18 Biosciences Biosciences NNP 10_1101-2021_01_07_425723 342 19 , , , 10_1101-2021_01_07_425723 342 20 Franklin Franklin NNP 10_1101-2021_01_07_425723 342 21 Lakes Lakes NNP 10_1101-2021_01_07_425723 342 22 , , , 10_1101-2021_01_07_425723 342 23 NJ NJ NNP 10_1101-2021_01_07_425723 342 24 ) ) -RRB- 10_1101-2021_01_07_425723 342 25 with with IN 10_1101-2021_01_07_425723 342 26 a a DT 10_1101-2021_01_07_425723 342 27 488 488 CD 10_1101-2021_01_07_425723 342 28 nm nm NNP 10_1101-2021_01_07_425723 342 29 laser laser NN 10_1101-2021_01_07_425723 342 30 and and CC 10_1101-2021_01_07_425723 342 31 fluorescence fluorescence NN 10_1101-2021_01_07_425723 342 32 was be VBD 10_1101-2021_01_07_425723 342 33 measured measure VBN 10_1101-2021_01_07_425723 342 34 with with IN 10_1101-2021_01_07_425723 342 35 emission emission NN 10_1101-2021_01_07_425723 342 36 524 524 CD 10_1101-2021_01_07_425723 342 37 filter filter NN 10_1101-2021_01_07_425723 342 38 of of IN 10_1101-2021_01_07_425723 342 39 533/30 533/30 CD 10_1101-2021_01_07_425723 342 40 nm nm IN 10_1101-2021_01_07_425723 342 41 ( ( -LRB- 10_1101-2021_01_07_425723 342 42 FL1 FL1 NNP 10_1101-2021_01_07_425723 342 43 ) ) -RRB- 10_1101-2021_01_07_425723 342 44 for for IN 10_1101-2021_01_07_425723 342 45 NBD NBD NNP 10_1101-2021_01_07_425723 342 46 - - HYPH 10_1101-2021_01_07_425723 342 47 cholesterol cholesterol NN 10_1101-2021_01_07_425723 342 48 and and CC 10_1101-2021_01_07_425723 342 49 > > XX 10_1101-2021_01_07_425723 342 50 670 670 CD 10_1101-2021_01_07_425723 342 51 nm nm CD 10_1101-2021_01_07_425723 342 52 ( ( -LRB- 10_1101-2021_01_07_425723 342 53 FL3 FL3 NNP 10_1101-2021_01_07_425723 342 54 ) ) -RRB- 10_1101-2021_01_07_425723 342 55 for for IN 10_1101-2021_01_07_425723 342 56 PI PI NNP 10_1101-2021_01_07_425723 342 57 . . . 10_1101-2021_01_07_425723 343 1 Cell cell NN 10_1101-2021_01_07_425723 343 2 gating gate VBG 10_1101-2021_01_07_425723 343 3 and and CC 10_1101-2021_01_07_425723 343 4 median median JJ 10_1101-2021_01_07_425723 343 5 525 525 CD 10_1101-2021_01_07_425723 343 6 fluorescence fluorescence NN 10_1101-2021_01_07_425723 343 7 of of IN 10_1101-2021_01_07_425723 343 8 cells cell NNS 10_1101-2021_01_07_425723 343 9 were be VBD 10_1101-2021_01_07_425723 343 10 determined determine VBN 10_1101-2021_01_07_425723 343 11 using use VBG 10_1101-2021_01_07_425723 343 12 FlowJo FlowJo NNP 10_1101-2021_01_07_425723 343 13 ( ( -LRB- 10_1101-2021_01_07_425723 343 14 v10 v10 NN 10_1101-2021_01_07_425723 343 15 , , , 10_1101-2021_01_07_425723 343 16 BD BD NNP 10_1101-2021_01_07_425723 343 17 Bioscience Bioscience NNP 10_1101-2021_01_07_425723 343 18 ) ) -RRB- 10_1101-2021_01_07_425723 343 19 . . . 10_1101-2021_01_07_425723 344 1 Cells cell NNS 10_1101-2021_01_07_425723 344 2 were be VBD 10_1101-2021_01_07_425723 344 3 gated gate VBN 10_1101-2021_01_07_425723 344 4 based base VBN 10_1101-2021_01_07_425723 344 5 on on IN 10_1101-2021_01_07_425723 344 6 526 526 CD 10_1101-2021_01_07_425723 344 7 forward forward JJ 10_1101-2021_01_07_425723 344 8 side side NN 10_1101-2021_01_07_425723 344 9 scatter scatter NN 10_1101-2021_01_07_425723 344 10 ( ( -LRB- 10_1101-2021_01_07_425723 344 11 FSC FSC NNP 10_1101-2021_01_07_425723 344 12 ) ) -RRB- 10_1101-2021_01_07_425723 344 13 and and CC 10_1101-2021_01_07_425723 344 14 side side NN 10_1101-2021_01_07_425723 344 15 - - HYPH 10_1101-2021_01_07_425723 344 16 scatter scatter NN 10_1101-2021_01_07_425723 344 17 ( ( -LRB- 10_1101-2021_01_07_425723 344 18 SSC SSC NNP 10_1101-2021_01_07_425723 344 19 ) ) -RRB- 10_1101-2021_01_07_425723 344 20 to to TO 10_1101-2021_01_07_425723 344 21 exclude exclude VB 10_1101-2021_01_07_425723 344 22 potential potential JJ 10_1101-2021_01_07_425723 344 23 artifacts artifact NNS 10_1101-2021_01_07_425723 344 24 or or CC 10_1101-2021_01_07_425723 344 25 clumping clump VBG 10_1101-2021_01_07_425723 344 26 cells cell NNS 10_1101-2021_01_07_425723 344 27 . . . 10_1101-2021_01_07_425723 345 1 Within within IN 10_1101-2021_01_07_425723 345 2 527 527 CD 10_1101-2021_01_07_425723 345 3 this this DT 10_1101-2021_01_07_425723 345 4 gated gate VBN 10_1101-2021_01_07_425723 345 5 population population NN 10_1101-2021_01_07_425723 345 6 PI pi IN 10_1101-2021_01_07_425723 345 7 positive positive JJ 10_1101-2021_01_07_425723 345 8 and and CC 10_1101-2021_01_07_425723 345 9 negatively negatively RB 10_1101-2021_01_07_425723 345 10 stained stain VBN 10_1101-2021_01_07_425723 345 11 cells cell NNS 10_1101-2021_01_07_425723 345 12 were be VBD 10_1101-2021_01_07_425723 345 13 differentiated differentiate VBN 10_1101-2021_01_07_425723 345 14 based base VBN 10_1101-2021_01_07_425723 345 15 on on IN 10_1101-2021_01_07_425723 345 16 the the DT 10_1101-2021_01_07_425723 345 17 cell cell NN 10_1101-2021_01_07_425723 345 18 528 528 CD 10_1101-2021_01_07_425723 345 19 fluorescence fluorescence NN 10_1101-2021_01_07_425723 345 20 across across IN 10_1101-2021_01_07_425723 345 21 a a DT 10_1101-2021_01_07_425723 345 22 FL3 FL3 NNP 10_1101-2021_01_07_425723 345 23 FL1 FL1 NNP 10_1101-2021_01_07_425723 345 24 dimension dimension NN 10_1101-2021_01_07_425723 345 25 . . . 10_1101-2021_01_07_425723 346 1 Flow flow VB 10_1101-2021_01_07_425723 346 2 cytometric cytometric NN 10_1101-2021_01_07_425723 346 3 gates gate NNS 10_1101-2021_01_07_425723 346 4 were be VBD 10_1101-2021_01_07_425723 346 5 drafted draft VBN 10_1101-2021_01_07_425723 346 6 for for IN 10_1101-2021_01_07_425723 346 7 each each DT 10_1101-2021_01_07_425723 346 8 yeast yeast NN 10_1101-2021_01_07_425723 346 9 species specie NNS 10_1101-2021_01_07_425723 346 10 and and CC 10_1101-2021_01_07_425723 346 11 529 529 CD 10_1101-2021_01_07_425723 346 12 used use VBD 10_1101-2021_01_07_425723 346 13 for for IN 10_1101-2021_01_07_425723 346 14 all all DT 10_1101-2021_01_07_425723 346 15 samples sample NNS 10_1101-2021_01_07_425723 346 16 . . . 10_1101-2021_01_07_425723 347 1 The the DT 10_1101-2021_01_07_425723 347 2 gating gate VBG 10_1101-2021_01_07_425723 347 3 strategy strategy NN 10_1101-2021_01_07_425723 347 4 is be VBZ 10_1101-2021_01_07_425723 347 5 given give VBN 10_1101-2021_01_07_425723 347 6 in in IN 10_1101-2021_01_07_425723 347 7 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 347 8 Fig Fig NNP 10_1101-2021_01_07_425723 347 9 . . . 10_1101-2021_01_07_425723 348 1 8 8 LS 10_1101-2021_01_07_425723 348 2 . . . 10_1101-2021_01_07_425723 349 1 Fluorescence fluorescence NN 10_1101-2021_01_07_425723 349 2 of of IN 10_1101-2021_01_07_425723 349 3 a a DT 10_1101-2021_01_07_425723 349 4 strain strain NN 10_1101-2021_01_07_425723 349 5 was be VBD 10_1101-2021_01_07_425723 349 6 530 530 CD 10_1101-2021_01_07_425723 349 7 determined determine VBN 10_1101-2021_01_07_425723 349 8 by by IN 10_1101-2021_01_07_425723 349 9 a a DT 10_1101-2021_01_07_425723 349 10 sample sample NN 10_1101-2021_01_07_425723 349 11 of of IN 10_1101-2021_01_07_425723 349 12 cells cell NNS 10_1101-2021_01_07_425723 349 13 from from IN 10_1101-2021_01_07_425723 349 14 independent independent JJ 10_1101-2021_01_07_425723 349 15 shake shake NN 10_1101-2021_01_07_425723 349 16 - - HYPH 10_1101-2021_01_07_425723 349 17 flask flask NN 10_1101-2021_01_07_425723 349 18 cultures culture NNS 10_1101-2021_01_07_425723 349 19 and and CC 10_1101-2021_01_07_425723 349 20 compared compare VBN 10_1101-2021_01_07_425723 349 21 to to IN 10_1101-2021_01_07_425723 349 22 cells cell NNS 10_1101-2021_01_07_425723 349 23 from from IN 10_1101-2021_01_07_425723 349 24 531 531 CD 10_1101-2021_01_07_425723 349 25 identical identical JJ 10_1101-2021_01_07_425723 349 26 unstained unstained JJ 10_1101-2021_01_07_425723 349 27 cultures culture NNS 10_1101-2021_01_07_425723 349 28 of of IN 10_1101-2021_01_07_425723 349 29 cells cell NNS 10_1101-2021_01_07_425723 349 30 with with IN 10_1101-2021_01_07_425723 349 31 the the DT 10_1101-2021_01_07_425723 349 32 exact exact JJ 10_1101-2021_01_07_425723 349 33 same same JJ 10_1101-2021_01_07_425723 349 34 chronological chronological JJ 10_1101-2021_01_07_425723 349 35 age age NN 10_1101-2021_01_07_425723 349 36 . . . 10_1101-2021_01_07_425723 350 1 The the DT 10_1101-2021_01_07_425723 350 2 staining staining JJ 10_1101-2021_01_07_425723 350 3 experiment experiment NN 10_1101-2021_01_07_425723 350 4 of of IN 10_1101-2021_01_07_425723 350 5 532 532 CD 10_1101-2021_01_07_425723 350 6 the the DT 10_1101-2021_01_07_425723 350 7 strains strain NNS 10_1101-2021_01_07_425723 350 8 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 350 9 , , , 10_1101-2021_01_07_425723 350 10 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 350 11 and and CC 10_1101-2021_01_07_425723 350 12 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 350 13 samples sample NNS 10_1101-2021_01_07_425723 350 14 was be VBD 10_1101-2021_01_07_425723 350 15 repeated repeat VBN 10_1101-2021_01_07_425723 350 16 twice twice RB 10_1101-2021_01_07_425723 350 17 for for IN 10_1101-2021_01_07_425723 350 18 reproducibility reproducibility NN 10_1101-2021_01_07_425723 350 19 , , , 10_1101-2021_01_07_425723 350 20 the the DT 10_1101-2021_01_07_425723 350 21 mean mean NNP 10_1101-2021_01_07_425723 350 22 533 533 CD 10_1101-2021_01_07_425723 350 23 and and CC 10_1101-2021_01_07_425723 350 24 pooled pool VBN 10_1101-2021_01_07_425723 350 25 variance variance NN 10_1101-2021_01_07_425723 350 26 was be VBD 10_1101-2021_01_07_425723 350 27 subsequently subsequently RB 10_1101-2021_01_07_425723 350 28 calculated calculate VBN 10_1101-2021_01_07_425723 350 29 from from IN 10_1101-2021_01_07_425723 350 30 the the DT 10_1101-2021_01_07_425723 350 31 biological biological JJ 10_1101-2021_01_07_425723 350 32 duplicates duplicate NNS 10_1101-2021_01_07_425723 350 33 of of IN 10_1101-2021_01_07_425723 350 34 the the DT 10_1101-2021_01_07_425723 350 35 two two CD 10_1101-2021_01_07_425723 350 36 experiments experiment NNS 10_1101-2021_01_07_425723 350 37 . . . 10_1101-2021_01_07_425723 351 1 534 534 CD 10_1101-2021_01_07_425723 351 2 The the DT 10_1101-2021_01_07_425723 351 3 NBDC NBDC NNP 10_1101-2021_01_07_425723 351 4 intensity intensity NN 10_1101-2021_01_07_425723 351 5 and and CC 10_1101-2021_01_07_425723 351 6 cell cell NN 10_1101-2021_01_07_425723 351 7 counts count NNS 10_1101-2021_01_07_425723 351 8 obtained obtain VBN 10_1101-2021_01_07_425723 351 9 from from IN 10_1101-2021_01_07_425723 351 10 the the DT 10_1101-2021_01_07_425723 351 11 NBDC NBDC NNP 10_1101-2021_01_07_425723 351 12 experiments experiment NNS 10_1101-2021_01_07_425723 351 13 are be VBP 10_1101-2021_01_07_425723 351 14 available available JJ 10_1101-2021_01_07_425723 351 15 for for IN 10_1101-2021_01_07_425723 351 16 re re NN 10_1101-2021_01_07_425723 351 17 - - NN 10_1101-2021_01_07_425723 351 18 analysis analysis NN 10_1101-2021_01_07_425723 351 19 in in IN 10_1101-2021_01_07_425723 351 20 535 535 CD 10_1101-2021_01_07_425723 351 21 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 351 22 Data Data NNP 10_1101-2021_01_07_425723 351 23 set set NN 10_1101-2021_01_07_425723 351 24 1 1 CD 10_1101-2021_01_07_425723 351 25 , , , 10_1101-2021_01_07_425723 351 26 and and CC 10_1101-2021_01_07_425723 351 27 raw raw JJ 10_1101-2021_01_07_425723 351 28 flow flow NN 10_1101-2021_01_07_425723 351 29 cytometry cytometry NN 10_1101-2021_01_07_425723 351 30 plots plot NNS 10_1101-2021_01_07_425723 351 31 are be VBP 10_1101-2021_01_07_425723 351 32 depicted depict VBN 10_1101-2021_01_07_425723 351 33 in in IN 10_1101-2021_01_07_425723 351 34 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 351 35 Data Data NNP 10_1101-2021_01_07_425723 351 36 set set NN 10_1101-2021_01_07_425723 351 37 2 2 CD 10_1101-2021_01_07_425723 351 38 . . . 10_1101-2021_01_07_425723 352 1 536 536 CD 10_1101-2021_01_07_425723 352 2 Long long RB 10_1101-2021_01_07_425723 352 3 read read VBD 10_1101-2021_01_07_425723 352 4 sequencing sequencing NN 10_1101-2021_01_07_425723 352 5 , , , 10_1101-2021_01_07_425723 352 6 assembly assembly NN 10_1101-2021_01_07_425723 352 7 , , , 10_1101-2021_01_07_425723 352 8 and and CC 10_1101-2021_01_07_425723 352 9 annotation annotation NN 10_1101-2021_01_07_425723 352 10 537 537 CD 10_1101-2021_01_07_425723 352 11 Cells cell NNS 10_1101-2021_01_07_425723 352 12 were be VBD 10_1101-2021_01_07_425723 352 13 grown grow VBN 10_1101-2021_01_07_425723 352 14 overnight overnight RB 10_1101-2021_01_07_425723 352 15 in in IN 10_1101-2021_01_07_425723 352 16 500-mL 500-ml CD 10_1101-2021_01_07_425723 352 17 shake shake NN 10_1101-2021_01_07_425723 352 18 flasks flask NNS 10_1101-2021_01_07_425723 352 19 containing contain VBG 10_1101-2021_01_07_425723 352 20 100 100 CD 10_1101-2021_01_07_425723 352 21 mL mL NNP 10_1101-2021_01_07_425723 352 22 liquid liquid JJ 10_1101-2021_01_07_425723 352 23 YPD YPD NNP 10_1101-2021_01_07_425723 352 24 medium medium NN 10_1101-2021_01_07_425723 352 25 at at IN 10_1101-2021_01_07_425723 352 26 30 30 CD 10_1101-2021_01_07_425723 352 27 ° ° , 10_1101-2021_01_07_425723 352 28 C C NNP 10_1101-2021_01_07_425723 352 29 in in IN 10_1101-2021_01_07_425723 352 30 an an DT 10_1101-2021_01_07_425723 352 31 538 538 CD 10_1101-2021_01_07_425723 352 32 orbital orbital JJ 10_1101-2021_01_07_425723 352 33 shaker shaker NN 10_1101-2021_01_07_425723 352 34 at at IN 10_1101-2021_01_07_425723 352 35 200 200 CD 10_1101-2021_01_07_425723 352 36 rpm rpm NN 10_1101-2021_01_07_425723 352 37 . . . 10_1101-2021_01_07_425723 353 1 After after IN 10_1101-2021_01_07_425723 353 2 reaching reach VBG 10_1101-2021_01_07_425723 353 3 stationary stationary JJ 10_1101-2021_01_07_425723 353 4 phase phase NN 10_1101-2021_01_07_425723 353 5 the the DT 10_1101-2021_01_07_425723 353 6 cells cell NNS 10_1101-2021_01_07_425723 353 7 were be VBD 10_1101-2021_01_07_425723 353 8 harvested harvest VBN 10_1101-2021_01_07_425723 353 9 for for IN 10_1101-2021_01_07_425723 353 10 a a DT 10_1101-2021_01_07_425723 353 11 total total JJ 10_1101-2021_01_07_425723 353 12 OD660 od660 NN 10_1101-2021_01_07_425723 353 13 of of IN 10_1101-2021_01_07_425723 353 14 539 539 CD 10_1101-2021_01_07_425723 353 15 600 600 CD 10_1101-2021_01_07_425723 353 16 by by IN 10_1101-2021_01_07_425723 353 17 centrifugation centrifugation NN 10_1101-2021_01_07_425723 353 18 for for IN 10_1101-2021_01_07_425723 353 19 5 5 CD 10_1101-2021_01_07_425723 353 20 min min NN 10_1101-2021_01_07_425723 353 21 at at IN 10_1101-2021_01_07_425723 353 22 4000 4000 CD 10_1101-2021_01_07_425723 353 23 g. g. NNP 10_1101-2021_01_07_425723 353 24 Genomic Genomic NNP 10_1101-2021_01_07_425723 353 25 DNA DNA NNP 10_1101-2021_01_07_425723 353 26 of of IN 10_1101-2021_01_07_425723 353 27 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 353 28 and and CC 10_1101-2021_01_07_425723 353 29 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 353 30 was be VBD 10_1101-2021_01_07_425723 353 31 isolated isolate VBN 10_1101-2021_01_07_425723 353 32 using use VBG 10_1101-2021_01_07_425723 353 33 540 540 CD 10_1101-2021_01_07_425723 353 34 .CC .CC NFP 10_1101-2021_01_07_425723 353 35 - - : 10_1101-2021_01_07_425723 353 36 BY by IN 10_1101-2021_01_07_425723 353 37 - - HYPH 10_1101-2021_01_07_425723 353 38 NC NC NNP 10_1101-2021_01_07_425723 353 39 - - HYPH 10_1101-2021_01_07_425723 353 40 ND ND NNP 10_1101-2021_01_07_425723 353 41 4.0 4.0 CD 10_1101-2021_01_07_425723 353 42 International International NNP 10_1101-2021_01_07_425723 353 43 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 353 44 under under IN 10_1101-2021_01_07_425723 353 45 a a DT 10_1101-2021_01_07_425723 353 46 ( ( -LRB- 10_1101-2021_01_07_425723 353 47 which which WDT 10_1101-2021_01_07_425723 353 48 was be VBD 10_1101-2021_01_07_425723 353 49 not not RB 10_1101-2021_01_07_425723 353 50 certified certify VBN 10_1101-2021_01_07_425723 353 51 by by IN 10_1101-2021_01_07_425723 353 52 peer peer NN 10_1101-2021_01_07_425723 353 53 review review NN 10_1101-2021_01_07_425723 353 54 ) ) -RRB- 10_1101-2021_01_07_425723 353 55 is be VBZ 10_1101-2021_01_07_425723 353 56 the the DT 10_1101-2021_01_07_425723 353 57 author author NN 10_1101-2021_01_07_425723 353 58 / / SYM 10_1101-2021_01_07_425723 353 59 funder funder NN 10_1101-2021_01_07_425723 353 60 , , , 10_1101-2021_01_07_425723 353 61 who who WP 10_1101-2021_01_07_425723 353 62 has have VBZ 10_1101-2021_01_07_425723 353 63 granted grant VBN 10_1101-2021_01_07_425723 353 64 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 353 65 a a DT 10_1101-2021_01_07_425723 353 66 license license NN 10_1101-2021_01_07_425723 353 67 to to TO 10_1101-2021_01_07_425723 353 68 display display VB 10_1101-2021_01_07_425723 353 69 the the DT 10_1101-2021_01_07_425723 353 70 preprint preprint NN 10_1101-2021_01_07_425723 353 71 in in IN 10_1101-2021_01_07_425723 353 72 perpetuity perpetuity NN 10_1101-2021_01_07_425723 353 73 . . . 10_1101-2021_01_07_425723 354 1 It -PRON- PRP 10_1101-2021_01_07_425723 354 2 is be VBZ 10_1101-2021_01_07_425723 354 3 made make VBN 10_1101-2021_01_07_425723 354 4 The the DT 10_1101-2021_01_07_425723 354 5 copyright copyright NN 10_1101-2021_01_07_425723 354 6 holder holder NN 10_1101-2021_01_07_425723 354 7 for for IN 10_1101-2021_01_07_425723 354 8 this this DT 10_1101-2021_01_07_425723 354 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 354 10 version version NN 10_1101-2021_01_07_425723 354 11 posted post VBD 10_1101-2021_01_07_425723 354 12 January January NNP 10_1101-2021_01_07_425723 354 13 8 8 CD 10_1101-2021_01_07_425723 354 14 , , , 10_1101-2021_01_07_425723 354 15 2021 2021 CD 10_1101-2021_01_07_425723 354 16 . . . 10_1101-2021_01_07_425723 354 17 ; ; : 10_1101-2021_01_07_425723 354 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 354 19 : : : 10_1101-2021_01_07_425723 354 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 354 21 preprint preprint NN 10_1101-2021_01_07_425723 354 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 354 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425723 354 24 32 32 CD 10_1101-2021_01_07_425723 354 25 the the DT 10_1101-2021_01_07_425723 354 26 Qiagen Qiagen NNP 10_1101-2021_01_07_425723 354 27 genomic genomic JJ 10_1101-2021_01_07_425723 354 28 DNA DNA NNP 10_1101-2021_01_07_425723 354 29 100 100 CD 10_1101-2021_01_07_425723 354 30 / / SYM 10_1101-2021_01_07_425723 354 31 G g NN 10_1101-2021_01_07_425723 354 32 kit kit NN 10_1101-2021_01_07_425723 354 33 ( ( -LRB- 10_1101-2021_01_07_425723 354 34 Qiagen Qiagen NNP 10_1101-2021_01_07_425723 354 35 , , , 10_1101-2021_01_07_425723 354 36 Hilden Hilden NNP 10_1101-2021_01_07_425723 354 37 , , , 10_1101-2021_01_07_425723 354 38 Germany Germany NNP 10_1101-2021_01_07_425723 354 39 ) ) -RRB- 10_1101-2021_01_07_425723 354 40 according accord VBG 10_1101-2021_01_07_425723 354 41 to to IN 10_1101-2021_01_07_425723 354 42 the the DT 10_1101-2021_01_07_425723 354 43 manufacturer manufacturer NN 10_1101-2021_01_07_425723 354 44 ’s ’s POS 10_1101-2021_01_07_425723 354 45 541 541 CD 10_1101-2021_01_07_425723 354 46 instructions instruction NNS 10_1101-2021_01_07_425723 354 47 . . . 10_1101-2021_01_07_425723 355 1 MinION MinION NNP 10_1101-2021_01_07_425723 355 2 genomic genomic JJ 10_1101-2021_01_07_425723 355 3 libraries library NNS 10_1101-2021_01_07_425723 355 4 were be VBD 10_1101-2021_01_07_425723 355 5 prepared prepare VBN 10_1101-2021_01_07_425723 355 6 using use VBG 10_1101-2021_01_07_425723 355 7 the the DT 10_1101-2021_01_07_425723 355 8 1D 1d JJ 10_1101-2021_01_07_425723 355 9 Genomic Genomic NNP 10_1101-2021_01_07_425723 355 10 DNA DNA NNP 10_1101-2021_01_07_425723 355 11 by by IN 10_1101-2021_01_07_425723 355 12 ligation ligation NN 10_1101-2021_01_07_425723 355 13 ( ( -LRB- 10_1101-2021_01_07_425723 355 14 SQK-542 sqk-542 XX 10_1101-2021_01_07_425723 355 15 LSK108 LSK108 NNP 10_1101-2021_01_07_425723 355 16 ) ) -RRB- 10_1101-2021_01_07_425723 355 17 for for IN 10_1101-2021_01_07_425723 355 18 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 355 19 , , , 10_1101-2021_01_07_425723 355 20 and and CC 10_1101-2021_01_07_425723 355 21 the the DT 10_1101-2021_01_07_425723 355 22 1D 1D NNP 10_1101-2021_01_07_425723 355 23 native native JJ 10_1101-2021_01_07_425723 355 24 barcoding barcode VBG 10_1101-2021_01_07_425723 355 25 Genomic Genomic NNP 10_1101-2021_01_07_425723 355 26 DNA DNA NNP 10_1101-2021_01_07_425723 355 27 ( ( -LRB- 10_1101-2021_01_07_425723 355 28 EXP EXP NNP 10_1101-2021_01_07_425723 355 29 - - HYPH 10_1101-2021_01_07_425723 355 30 NBD103 NBD103 NNP 10_1101-2021_01_07_425723 355 31 & & CC 10_1101-2021_01_07_425723 355 32 LSK108 LSK108 NNP 10_1101-2021_01_07_425723 355 33 ) ) -RRB- 10_1101-2021_01_07_425723 355 34 for for IN 10_1101-2021_01_07_425723 355 35 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 355 36 543 543 CD 10_1101-2021_01_07_425723 355 37 according accord VBG 10_1101-2021_01_07_425723 355 38 to to IN 10_1101-2021_01_07_425723 355 39 the the DT 10_1101-2021_01_07_425723 355 40 manufacturer manufacturer NN 10_1101-2021_01_07_425723 355 41 ’s ’s POS 10_1101-2021_01_07_425723 355 42 instructions instruction NNS 10_1101-2021_01_07_425723 355 43 with with IN 10_1101-2021_01_07_425723 355 44 the the DT 10_1101-2021_01_07_425723 355 45 exception exception NN 10_1101-2021_01_07_425723 355 46 of of IN 10_1101-2021_01_07_425723 355 47 using use VBG 10_1101-2021_01_07_425723 355 48 80 80 CD 10_1101-2021_01_07_425723 355 49 % % NN 10_1101-2021_01_07_425723 355 50 EtOH etoh NN 10_1101-2021_01_07_425723 355 51 during during IN 10_1101-2021_01_07_425723 355 52 the the DT 10_1101-2021_01_07_425723 355 53 ‘ ' `` 10_1101-2021_01_07_425723 355 54 End End NNP 10_1101-2021_01_07_425723 355 55 544 544 CD 10_1101-2021_01_07_425723 355 56 Repair repair NN 10_1101-2021_01_07_425723 355 57 / / SYM 10_1101-2021_01_07_425723 355 58 dA dA NNP 10_1101-2021_01_07_425723 355 59 - - HYPH 10_1101-2021_01_07_425723 355 60 tailing tail VBG 10_1101-2021_01_07_425723 355 61 module module NN 10_1101-2021_01_07_425723 355 62 ’ ' '' 10_1101-2021_01_07_425723 355 63 step step NN 10_1101-2021_01_07_425723 355 64 . . . 10_1101-2021_01_07_425723 356 1 Flow flow JJ 10_1101-2021_01_07_425723 356 2 cell cell NN 10_1101-2021_01_07_425723 356 3 quality quality NN 10_1101-2021_01_07_425723 356 4 was be VBD 10_1101-2021_01_07_425723 356 5 tested test VBN 10_1101-2021_01_07_425723 356 6 by by IN 10_1101-2021_01_07_425723 356 7 running run VBG 10_1101-2021_01_07_425723 356 8 the the DT 10_1101-2021_01_07_425723 356 9 MinKNOW MinKNOW NNP 10_1101-2021_01_07_425723 356 10 platform platform NN 10_1101-2021_01_07_425723 356 11 QC QC NNP 10_1101-2021_01_07_425723 356 12 545 545 CD 10_1101-2021_01_07_425723 356 13 ( ( -LRB- 10_1101-2021_01_07_425723 356 14 Oxford Oxford NNP 10_1101-2021_01_07_425723 356 15 Nanopore Nanopore NNP 10_1101-2021_01_07_425723 356 16 Technology Technology NNP 10_1101-2021_01_07_425723 356 17 , , , 10_1101-2021_01_07_425723 356 18 Oxford Oxford NNP 10_1101-2021_01_07_425723 356 19 , , , 10_1101-2021_01_07_425723 356 20 UK UK NNP 10_1101-2021_01_07_425723 356 21 ) ) -RRB- 10_1101-2021_01_07_425723 356 22 . . . 10_1101-2021_01_07_425723 357 1 Flow flow NN 10_1101-2021_01_07_425723 357 2 cells cell NNS 10_1101-2021_01_07_425723 357 3 were be VBD 10_1101-2021_01_07_425723 357 4 prepared prepare VBN 10_1101-2021_01_07_425723 357 5 by by IN 10_1101-2021_01_07_425723 357 6 removing remove VBG 10_1101-2021_01_07_425723 357 7 20 20 CD 10_1101-2021_01_07_425723 357 8 μL μL NNP 10_1101-2021_01_07_425723 357 9 buffer buffer NNP 10_1101-2021_01_07_425723 357 10 and and CC 10_1101-2021_01_07_425723 357 11 546 546 CD 10_1101-2021_01_07_425723 357 12 subsequently subsequently RB 10_1101-2021_01_07_425723 357 13 primed prime VBN 10_1101-2021_01_07_425723 357 14 with with IN 10_1101-2021_01_07_425723 357 15 priming priming NN 10_1101-2021_01_07_425723 357 16 buffer buffer NN 10_1101-2021_01_07_425723 357 17 . . . 10_1101-2021_01_07_425723 358 1 The the DT 10_1101-2021_01_07_425723 358 2 DNA dna NN 10_1101-2021_01_07_425723 358 3 library library NN 10_1101-2021_01_07_425723 358 4 was be VBD 10_1101-2021_01_07_425723 358 5 loaded load VBN 10_1101-2021_01_07_425723 358 6 dropwise dropwise NN 10_1101-2021_01_07_425723 358 7 into into IN 10_1101-2021_01_07_425723 358 8 the the DT 10_1101-2021_01_07_425723 358 9 flow flow NN 10_1101-2021_01_07_425723 358 10 cell cell NN 10_1101-2021_01_07_425723 358 11 for for IN 10_1101-2021_01_07_425723 358 12 547 547 CD 10_1101-2021_01_07_425723 358 13 sequencing sequencing NN 10_1101-2021_01_07_425723 358 14 . . . 10_1101-2021_01_07_425723 359 1 The the DT 10_1101-2021_01_07_425723 359 2 SQK SQK NNP 10_1101-2021_01_07_425723 359 3 - - HYPH 10_1101-2021_01_07_425723 359 4 LSK108 LSK108 NNP 10_1101-2021_01_07_425723 359 5 library library NN 10_1101-2021_01_07_425723 359 6 was be VBD 10_1101-2021_01_07_425723 359 7 sequenced sequence VBN 10_1101-2021_01_07_425723 359 8 on on IN 10_1101-2021_01_07_425723 359 9 a a DT 10_1101-2021_01_07_425723 359 10 R9 r9 JJ 10_1101-2021_01_07_425723 359 11 chemistry chemistry NN 10_1101-2021_01_07_425723 359 12 flow flow NN 10_1101-2021_01_07_425723 359 13 cell cell NN 10_1101-2021_01_07_425723 359 14 ( ( -LRB- 10_1101-2021_01_07_425723 359 15 FLO FLO NNP 10_1101-2021_01_07_425723 359 16 - - HYPH 10_1101-2021_01_07_425723 359 17 MIN106 MIN106 NNP 10_1101-2021_01_07_425723 359 18 ) ) -RRB- 10_1101-2021_01_07_425723 359 19 for for IN 10_1101-2021_01_07_425723 359 20 48 48 CD 10_1101-2021_01_07_425723 359 21 h. h. NN 10_1101-2021_01_07_425723 359 22 548 548 CD 10_1101-2021_01_07_425723 359 23 Base Base NNP 10_1101-2021_01_07_425723 359 24 - - HYPH 10_1101-2021_01_07_425723 359 25 calling calling NN 10_1101-2021_01_07_425723 359 26 was be VBD 10_1101-2021_01_07_425723 359 27 performed perform VBN 10_1101-2021_01_07_425723 359 28 using use VBG 10_1101-2021_01_07_425723 359 29 Albacore Albacore NNP 10_1101-2021_01_07_425723 359 30 ( ( -LRB- 10_1101-2021_01_07_425723 359 31 v2.3.1 v2.3.1 NNP 10_1101-2021_01_07_425723 359 32 , , , 10_1101-2021_01_07_425723 359 33 Oxford Oxford NNP 10_1101-2021_01_07_425723 359 34 Nanopore Nanopore NNP 10_1101-2021_01_07_425723 359 35 Technologies Technologies NNPS 10_1101-2021_01_07_425723 359 36 ) ) -RRB- 10_1101-2021_01_07_425723 359 37 for for IN 10_1101-2021_01_07_425723 359 38 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 359 39 , , , 10_1101-2021_01_07_425723 359 40 and and CC 10_1101-2021_01_07_425723 359 41 for for IN 10_1101-2021_01_07_425723 359 42 549 549 CD 10_1101-2021_01_07_425723 359 43 NBRC1777 NBRC1777 NNPS 10_1101-2021_01_07_425723 359 44 with with IN 10_1101-2021_01_07_425723 359 45 Guppy Guppy NNP 10_1101-2021_01_07_425723 359 46 ( ( -LRB- 10_1101-2021_01_07_425723 359 47 v2.1.3 v2.1.3 NNP 10_1101-2021_01_07_425723 359 48 , , , 10_1101-2021_01_07_425723 359 49 Oxford Oxford NNP 10_1101-2021_01_07_425723 359 50 Nanopore Nanopore NNP 10_1101-2021_01_07_425723 359 51 Technologies Technologies NNPS 10_1101-2021_01_07_425723 359 52 ) ) -RRB- 10_1101-2021_01_07_425723 359 53 using use VBG 10_1101-2021_01_07_425723 359 54 dna_r9.4.1_450bps_flipflop.cfg dna_r9.4.1_450bps_flipflop.cfg NNP 10_1101-2021_01_07_425723 359 55 . . . 10_1101-2021_01_07_425723 360 1 550 550 CD 10_1101-2021_01_07_425723 360 2 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 360 3 reads read NNS 10_1101-2021_01_07_425723 360 4 were be VBD 10_1101-2021_01_07_425723 360 5 assembled assemble VBN 10_1101-2021_01_07_425723 360 6 using use VBG 10_1101-2021_01_07_425723 360 7 Canu Canu NNP 10_1101-2021_01_07_425723 360 8 ( ( -LRB- 10_1101-2021_01_07_425723 360 9 v1.8)69 v1.8)69 : 10_1101-2021_01_07_425723 360 10 , , , 10_1101-2021_01_07_425723 360 11 and and CC 10_1101-2021_01_07_425723 360 12 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 360 13 reads read NNS 10_1101-2021_01_07_425723 360 14 were be VBD 10_1101-2021_01_07_425723 360 15 assembled assemble VBN 10_1101-2021_01_07_425723 360 16 using use VBG 10_1101-2021_01_07_425723 360 17 Flye Flye NNP 10_1101-2021_01_07_425723 360 18 551 551 CD 10_1101-2021_01_07_425723 360 19 ( ( -LRB- 10_1101-2021_01_07_425723 360 20 v2.7.1-b1673)70 v2.7.1-b1673)70 FW 10_1101-2021_01_07_425723 360 21 . . . 10_1101-2021_01_07_425723 361 1 Assemblies assembly NNS 10_1101-2021_01_07_425723 361 2 were be VBD 10_1101-2021_01_07_425723 361 3 polished polish VBN 10_1101-2021_01_07_425723 361 4 with with IN 10_1101-2021_01_07_425723 361 5 Pilon Pilon NNP 10_1101-2021_01_07_425723 361 6 ( ( -LRB- 10_1101-2021_01_07_425723 361 7 v1.18)71 v1.18)71 NNP 10_1101-2021_01_07_425723 361 8 using use VBG 10_1101-2021_01_07_425723 361 9 Illumina Illumina NNP 10_1101-2021_01_07_425723 361 10 data datum NNS 10_1101-2021_01_07_425723 361 11 available available JJ 10_1101-2021_01_07_425723 361 12 at at IN 10_1101-2021_01_07_425723 361 13 the the DT 10_1101-2021_01_07_425723 361 14 552 552 CD 10_1101-2021_01_07_425723 361 15 Sequence Sequence NNP 10_1101-2021_01_07_425723 361 16 Read Read VBD 10_1101-2021_01_07_425723 361 17 Archive Archive NNP 10_1101-2021_01_07_425723 361 18 under under IN 10_1101-2021_01_07_425723 361 19 accessions accession NNS 10_1101-2021_01_07_425723 361 20 SRX3637961 SRX3637961 NNP 10_1101-2021_01_07_425723 361 21 and and CC 10_1101-2021_01_07_425723 361 22 SRX3541357 SRX3541357 NNP 10_1101-2021_01_07_425723 361 23 . . . 10_1101-2021_01_07_425723 362 1 Both both DT 10_1101-2021_01_07_425723 362 2 de de IN 10_1101-2021_01_07_425723 362 3 novo novo NNP 10_1101-2021_01_07_425723 362 4 genome genome NNP 10_1101-2021_01_07_425723 362 5 553 553 CD 10_1101-2021_01_07_425723 362 6 assemblies assembly NNS 10_1101-2021_01_07_425723 362 7 were be VBD 10_1101-2021_01_07_425723 362 8 annotated annotate VBN 10_1101-2021_01_07_425723 362 9 using use VBG 10_1101-2021_01_07_425723 362 10 Funannotate Funannotate NNP 10_1101-2021_01_07_425723 362 11 ( ( -LRB- 10_1101-2021_01_07_425723 362 12 v1.7.1)72 v1.7.1)72 NNP 10_1101-2021_01_07_425723 362 13 , , , 10_1101-2021_01_07_425723 362 14 trained trained JJ 10_1101-2021_01_07_425723 362 15 and and CC 10_1101-2021_01_07_425723 362 16 refined refine VBN 10_1101-2021_01_07_425723 362 17 using use VBG 10_1101-2021_01_07_425723 362 18 de de NNP 10_1101-2021_01_07_425723 362 19 novo novo NNP 10_1101-2021_01_07_425723 362 20 554 554 CD 10_1101-2021_01_07_425723 362 21 transcriptome transcriptome DT 10_1101-2021_01_07_425723 362 22 assemblies assembly NNS 10_1101-2021_01_07_425723 362 23 ( ( -LRB- 10_1101-2021_01_07_425723 362 24 see see VB 10_1101-2021_01_07_425723 362 25 below below RB 10_1101-2021_01_07_425723 362 26 ) ) -RRB- 10_1101-2021_01_07_425723 362 27 , , , 10_1101-2021_01_07_425723 362 28 adding add VBG 10_1101-2021_01_07_425723 362 29 functional functional JJ 10_1101-2021_01_07_425723 362 30 annotation annotation NN 10_1101-2021_01_07_425723 362 31 with with IN 10_1101-2021_01_07_425723 362 32 Interproscan Interproscan NNP 10_1101-2021_01_07_425723 362 33 ( ( -LRB- 10_1101-2021_01_07_425723 362 34 v5.25 v5.25 NN 10_1101-2021_01_07_425723 362 35 - - HYPH 10_1101-2021_01_07_425723 362 36 64.0)73 64.0)73 NN 10_1101-2021_01_07_425723 362 37 . . . 10_1101-2021_01_07_425723 363 1 555 555 CD 10_1101-2021_01_07_425723 363 2 Illumina Illumina NNP 10_1101-2021_01_07_425723 363 3 sequencing sequence VBG 10_1101-2021_01_07_425723 363 4 556 556 CD 10_1101-2021_01_07_425723 363 5 Plasmids Plasmids NNPS 10_1101-2021_01_07_425723 363 6 were be VBD 10_1101-2021_01_07_425723 363 7 sequenced sequence VBN 10_1101-2021_01_07_425723 363 8 on on IN 10_1101-2021_01_07_425723 363 9 a a DT 10_1101-2021_01_07_425723 363 10 MiniSeq MiniSeq NNP 10_1101-2021_01_07_425723 363 11 ( ( -LRB- 10_1101-2021_01_07_425723 363 12 Illumina Illumina NNP 10_1101-2021_01_07_425723 363 13 , , , 10_1101-2021_01_07_425723 363 14 San San NNP 10_1101-2021_01_07_425723 363 15 Diego Diego NNP 10_1101-2021_01_07_425723 363 16 , , , 10_1101-2021_01_07_425723 363 17 CA CA NNP 10_1101-2021_01_07_425723 363 18 ) ) -RRB- 10_1101-2021_01_07_425723 363 19 platform platform NN 10_1101-2021_01_07_425723 363 20 . . . 10_1101-2021_01_07_425723 364 1 Library library JJ 10_1101-2021_01_07_425723 364 2 preparation preparation NN 10_1101-2021_01_07_425723 364 3 was be VBD 10_1101-2021_01_07_425723 364 4 557 557 CD 10_1101-2021_01_07_425723 364 5 performed perform VBN 10_1101-2021_01_07_425723 364 6 with with IN 10_1101-2021_01_07_425723 364 7 Nextera Nextera NNP 10_1101-2021_01_07_425723 364 8 XT XT NNP 10_1101-2021_01_07_425723 364 9 DNA dna NN 10_1101-2021_01_07_425723 364 10 library library NN 10_1101-2021_01_07_425723 364 11 preparation preparation NN 10_1101-2021_01_07_425723 364 12 according accord VBG 10_1101-2021_01_07_425723 364 13 to to IN 10_1101-2021_01_07_425723 364 14 the the DT 10_1101-2021_01_07_425723 364 15 manufacturer manufacturer NN 10_1101-2021_01_07_425723 364 16 ’s ’s POS 10_1101-2021_01_07_425723 364 17 instructions instruction NNS 10_1101-2021_01_07_425723 364 18 558 558 CD 10_1101-2021_01_07_425723 364 19 ( ( -LRB- 10_1101-2021_01_07_425723 364 20 Illumina Illumina NNP 10_1101-2021_01_07_425723 364 21 ) ) -RRB- 10_1101-2021_01_07_425723 364 22 . . . 10_1101-2021_01_07_425723 365 1 The the DT 10_1101-2021_01_07_425723 365 2 library library NN 10_1101-2021_01_07_425723 365 3 preparation preparation NN 10_1101-2021_01_07_425723 365 4 included include VBD 10_1101-2021_01_07_425723 365 5 the the DT 10_1101-2021_01_07_425723 365 6 MiniSeq MiniSeq NNP 10_1101-2021_01_07_425723 365 7 Mid Mid NNP 10_1101-2021_01_07_425723 365 8 Output Output NNP 10_1101-2021_01_07_425723 365 9 kit kit NN 10_1101-2021_01_07_425723 365 10 ( ( -LRB- 10_1101-2021_01_07_425723 365 11 300 300 CD 10_1101-2021_01_07_425723 365 12 cycles cycle NNS 10_1101-2021_01_07_425723 365 13 ) ) -RRB- 10_1101-2021_01_07_425723 365 14 and and CC 10_1101-2021_01_07_425723 365 15 the the DT 10_1101-2021_01_07_425723 365 16 input input NN 10_1101-2021_01_07_425723 365 17 & & CC 10_1101-2021_01_07_425723 365 18 final final JJ 10_1101-2021_01_07_425723 365 19 559 559 CD 10_1101-2021_01_07_425723 365 20 DNA DNA NNP 10_1101-2021_01_07_425723 365 21 was be VBD 10_1101-2021_01_07_425723 365 22 quantified quantify VBN 10_1101-2021_01_07_425723 365 23 with with IN 10_1101-2021_01_07_425723 365 24 the the DT 10_1101-2021_01_07_425723 365 25 Qubit qubit JJ 10_1101-2021_01_07_425723 365 26 HS hs NN 10_1101-2021_01_07_425723 365 27 dsDNA dsdna FW 10_1101-2021_01_07_425723 365 28 kit kit NN 10_1101-2021_01_07_425723 365 29 ( ( -LRB- 10_1101-2021_01_07_425723 365 30 Life Life NNP 10_1101-2021_01_07_425723 365 31 Technologies Technologies NNPS 10_1101-2021_01_07_425723 365 32 , , , 10_1101-2021_01_07_425723 365 33 Thermo Thermo NNP 10_1101-2021_01_07_425723 365 34 Fisher Fisher NNP 10_1101-2021_01_07_425723 365 35 Scientific Scientific NNP 10_1101-2021_01_07_425723 365 36 ) ) -RRB- 10_1101-2021_01_07_425723 365 37 . . . 10_1101-2021_01_07_425723 366 1 560 560 CD 10_1101-2021_01_07_425723 366 2 Nucleotide nucleotide JJ 10_1101-2021_01_07_425723 366 3 sequences sequence NNS 10_1101-2021_01_07_425723 366 4 were be VBD 10_1101-2021_01_07_425723 366 5 assembled assemble VBN 10_1101-2021_01_07_425723 366 6 with with IN 10_1101-2021_01_07_425723 366 7 SPAdes74 SPAdes74 NNP 10_1101-2021_01_07_425723 366 8 and and CC 10_1101-2021_01_07_425723 366 9 compared compare VBN 10_1101-2021_01_07_425723 366 10 to to IN 10_1101-2021_01_07_425723 366 11 the the DT 10_1101-2021_01_07_425723 366 12 intended intend VBN 10_1101-2021_01_07_425723 366 13 in in IN 10_1101-2021_01_07_425723 366 14 silico silico NNP 10_1101-2021_01_07_425723 366 15 DNA dna NN 10_1101-2021_01_07_425723 366 16 561 561 CD 10_1101-2021_01_07_425723 366 17 construct construct NN 10_1101-2021_01_07_425723 366 18 . . . 10_1101-2021_01_07_425723 367 1 For for IN 10_1101-2021_01_07_425723 367 2 whole whole JJ 10_1101-2021_01_07_425723 367 3 - - HYPH 10_1101-2021_01_07_425723 367 4 genome genome NN 10_1101-2021_01_07_425723 367 5 sequencing sequencing NN 10_1101-2021_01_07_425723 367 6 , , , 10_1101-2021_01_07_425723 367 7 yeast yeast NN 10_1101-2021_01_07_425723 367 8 cells cell NNS 10_1101-2021_01_07_425723 367 9 were be VBD 10_1101-2021_01_07_425723 367 10 harvested harvest VBN 10_1101-2021_01_07_425723 367 11 from from IN 10_1101-2021_01_07_425723 367 12 overnight overnight JJ 10_1101-2021_01_07_425723 367 13 cultures culture NNS 10_1101-2021_01_07_425723 367 14 and and CC 10_1101-2021_01_07_425723 367 15 DNA dna NN 10_1101-2021_01_07_425723 367 16 562 562 CD 10_1101-2021_01_07_425723 367 17 was be VBD 10_1101-2021_01_07_425723 367 18 isolated isolate VBN 10_1101-2021_01_07_425723 367 19 with with IN 10_1101-2021_01_07_425723 367 20 the the DT 10_1101-2021_01_07_425723 367 21 Qiagen Qiagen NNP 10_1101-2021_01_07_425723 367 22 genomic genomic JJ 10_1101-2021_01_07_425723 367 23 DNA DNA NNP 10_1101-2021_01_07_425723 367 24 100 100 CD 10_1101-2021_01_07_425723 367 25 / / SYM 10_1101-2021_01_07_425723 367 26 G g NN 10_1101-2021_01_07_425723 367 27 kit kit NN 10_1101-2021_01_07_425723 367 28 ( ( -LRB- 10_1101-2021_01_07_425723 367 29 Qiagen Qiagen NNP 10_1101-2021_01_07_425723 367 30 ) ) -RRB- 10_1101-2021_01_07_425723 367 31 as as IN 10_1101-2021_01_07_425723 367 32 described describe VBN 10_1101-2021_01_07_425723 367 33 earlier early RBR 10_1101-2021_01_07_425723 367 34 . . . 10_1101-2021_01_07_425723 368 1 DNA dna NN 10_1101-2021_01_07_425723 368 2 quantity quantity NN 10_1101-2021_01_07_425723 368 3 was be VBD 10_1101-2021_01_07_425723 368 4 563 563 CD 10_1101-2021_01_07_425723 368 5 .CC .CC : 10_1101-2021_01_07_425723 368 6 - - : 10_1101-2021_01_07_425723 368 7 BY by IN 10_1101-2021_01_07_425723 368 8 - - HYPH 10_1101-2021_01_07_425723 368 9 NC NC NNP 10_1101-2021_01_07_425723 368 10 - - HYPH 10_1101-2021_01_07_425723 368 11 ND ND NNP 10_1101-2021_01_07_425723 368 12 4.0 4.0 CD 10_1101-2021_01_07_425723 368 13 International International NNP 10_1101-2021_01_07_425723 368 14 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 368 15 under under IN 10_1101-2021_01_07_425723 368 16 a a DT 10_1101-2021_01_07_425723 368 17 ( ( -LRB- 10_1101-2021_01_07_425723 368 18 which which WDT 10_1101-2021_01_07_425723 368 19 was be VBD 10_1101-2021_01_07_425723 368 20 not not RB 10_1101-2021_01_07_425723 368 21 certified certify VBN 10_1101-2021_01_07_425723 368 22 by by IN 10_1101-2021_01_07_425723 368 23 peer peer NN 10_1101-2021_01_07_425723 368 24 review review NN 10_1101-2021_01_07_425723 368 25 ) ) -RRB- 10_1101-2021_01_07_425723 368 26 is be VBZ 10_1101-2021_01_07_425723 368 27 the the DT 10_1101-2021_01_07_425723 368 28 author author NN 10_1101-2021_01_07_425723 368 29 / / SYM 10_1101-2021_01_07_425723 368 30 funder funder NN 10_1101-2021_01_07_425723 368 31 , , , 10_1101-2021_01_07_425723 368 32 who who WP 10_1101-2021_01_07_425723 368 33 has have VBZ 10_1101-2021_01_07_425723 368 34 granted grant VBN 10_1101-2021_01_07_425723 368 35 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 368 36 a a DT 10_1101-2021_01_07_425723 368 37 license license NN 10_1101-2021_01_07_425723 368 38 to to TO 10_1101-2021_01_07_425723 368 39 display display VB 10_1101-2021_01_07_425723 368 40 the the DT 10_1101-2021_01_07_425723 368 41 preprint preprint NN 10_1101-2021_01_07_425723 368 42 in in IN 10_1101-2021_01_07_425723 368 43 perpetuity perpetuity NN 10_1101-2021_01_07_425723 368 44 . . . 10_1101-2021_01_07_425723 369 1 It -PRON- PRP 10_1101-2021_01_07_425723 369 2 is be VBZ 10_1101-2021_01_07_425723 369 3 made make VBN 10_1101-2021_01_07_425723 369 4 The the DT 10_1101-2021_01_07_425723 369 5 copyright copyright NN 10_1101-2021_01_07_425723 369 6 holder holder NN 10_1101-2021_01_07_425723 369 7 for for IN 10_1101-2021_01_07_425723 369 8 this this DT 10_1101-2021_01_07_425723 369 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 369 10 version version NN 10_1101-2021_01_07_425723 369 11 posted post VBD 10_1101-2021_01_07_425723 369 12 January January NNP 10_1101-2021_01_07_425723 369 13 8 8 CD 10_1101-2021_01_07_425723 369 14 , , , 10_1101-2021_01_07_425723 369 15 2021 2021 CD 10_1101-2021_01_07_425723 369 16 . . . 10_1101-2021_01_07_425723 369 17 ; ; : 10_1101-2021_01_07_425723 369 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 369 19 : : : 10_1101-2021_01_07_425723 369 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 369 21 preprint preprint NN 10_1101-2021_01_07_425723 369 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 369 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 369 24 33 33 CD 10_1101-2021_01_07_425723 369 25 measured measure VBN 10_1101-2021_01_07_425723 369 26 with with IN 10_1101-2021_01_07_425723 369 27 the the DT 10_1101-2021_01_07_425723 369 28 QuBit QuBit NNP 10_1101-2021_01_07_425723 369 29 BR br NN 10_1101-2021_01_07_425723 369 30 dsDNA dsdna JJ 10_1101-2021_01_07_425723 369 31 kit kit NN 10_1101-2021_01_07_425723 369 32 ( ( -LRB- 10_1101-2021_01_07_425723 369 33 Thermo Thermo NNP 10_1101-2021_01_07_425723 369 34 Fisher Fisher NNP 10_1101-2021_01_07_425723 369 35 Scientific Scientific NNP 10_1101-2021_01_07_425723 369 36 ) ) -RRB- 10_1101-2021_01_07_425723 369 37 . . . 10_1101-2021_01_07_425723 370 1 300 300 CD 10_1101-2021_01_07_425723 370 2 bp bp NNP 10_1101-2021_01_07_425723 370 3 paired pair VBN 10_1101-2021_01_07_425723 370 4 - - HYPH 10_1101-2021_01_07_425723 370 5 end end NN 10_1101-2021_01_07_425723 370 6 libraries library NNS 10_1101-2021_01_07_425723 370 7 were be VBD 10_1101-2021_01_07_425723 370 8 564 564 CD 10_1101-2021_01_07_425723 370 9 prepared prepare VBN 10_1101-2021_01_07_425723 370 10 with with IN 10_1101-2021_01_07_425723 370 11 the the DT 10_1101-2021_01_07_425723 370 12 TruSeq TruSeq NNP 10_1101-2021_01_07_425723 370 13 DNA DNA NNP 10_1101-2021_01_07_425723 370 14 PCR PCR NNP 10_1101-2021_01_07_425723 370 15 - - HYPH 10_1101-2021_01_07_425723 370 16 free free JJ 10_1101-2021_01_07_425723 370 17 library library NN 10_1101-2021_01_07_425723 370 18 prep prep NN 10_1101-2021_01_07_425723 370 19 kit kit NN 10_1101-2021_01_07_425723 370 20 ( ( -LRB- 10_1101-2021_01_07_425723 370 21 Illumina Illumina NNP 10_1101-2021_01_07_425723 370 22 ) ) -RRB- 10_1101-2021_01_07_425723 370 23 according accord VBG 10_1101-2021_01_07_425723 370 24 to to IN 10_1101-2021_01_07_425723 370 25 the the DT 10_1101-2021_01_07_425723 370 26 manufacturer manufacturer NN 10_1101-2021_01_07_425723 370 27 ’s ’s POS 10_1101-2021_01_07_425723 370 28 565 565 CD 10_1101-2021_01_07_425723 370 29 instructions instruction NNS 10_1101-2021_01_07_425723 370 30 . . . 10_1101-2021_01_07_425723 371 1 Short short JJ 10_1101-2021_01_07_425723 371 2 read read VB 10_1101-2021_01_07_425723 371 3 whole whole RB 10_1101-2021_01_07_425723 371 4 - - HYPH 10_1101-2021_01_07_425723 371 5 genome genome RB 10_1101-2021_01_07_425723 371 6 sequencing sequencing NN 10_1101-2021_01_07_425723 371 7 was be VBD 10_1101-2021_01_07_425723 371 8 performed perform VBN 10_1101-2021_01_07_425723 371 9 on on IN 10_1101-2021_01_07_425723 371 10 a a DT 10_1101-2021_01_07_425723 371 11 MiSeq MiSeq NNP 10_1101-2021_01_07_425723 371 12 platform platform NN 10_1101-2021_01_07_425723 371 13 ( ( -LRB- 10_1101-2021_01_07_425723 371 14 Illumina Illumina NNP 10_1101-2021_01_07_425723 371 15 ) ) -RRB- 10_1101-2021_01_07_425723 371 16 . . . 10_1101-2021_01_07_425723 372 1 566 566 CD 10_1101-2021_01_07_425723 372 2 RNA rna NN 10_1101-2021_01_07_425723 372 3 isolation isolation NN 10_1101-2021_01_07_425723 372 4 , , , 10_1101-2021_01_07_425723 372 5 sequencing sequencing NN 10_1101-2021_01_07_425723 372 6 and and CC 10_1101-2021_01_07_425723 372 7 transcriptome transcriptome DT 10_1101-2021_01_07_425723 372 8 analysis analysis NN 10_1101-2021_01_07_425723 372 9 567 567 CD 10_1101-2021_01_07_425723 372 10 Culture Culture NNP 10_1101-2021_01_07_425723 372 11 broth broth NN 10_1101-2021_01_07_425723 372 12 from from IN 10_1101-2021_01_07_425723 372 13 chemostat chemostat JJ 10_1101-2021_01_07_425723 372 14 cultures culture NNS 10_1101-2021_01_07_425723 372 15 was be VBD 10_1101-2021_01_07_425723 372 16 directly directly RB 10_1101-2021_01_07_425723 372 17 sampled sample VBN 10_1101-2021_01_07_425723 372 18 into into IN 10_1101-2021_01_07_425723 372 19 liquid liquid JJ 10_1101-2021_01_07_425723 372 20 nitrogen nitrogen NN 10_1101-2021_01_07_425723 372 21 to to TO 10_1101-2021_01_07_425723 372 22 prevent prevent VB 10_1101-2021_01_07_425723 372 23 mRNA mrna RP 10_1101-2021_01_07_425723 372 24 568 568 CD 10_1101-2021_01_07_425723 372 25 turnover turnover NN 10_1101-2021_01_07_425723 372 26 . . . 10_1101-2021_01_07_425723 373 1 The the DT 10_1101-2021_01_07_425723 373 2 cell cell NN 10_1101-2021_01_07_425723 373 3 cultures culture NNS 10_1101-2021_01_07_425723 373 4 were be VBD 10_1101-2021_01_07_425723 373 5 stored store VBN 10_1101-2021_01_07_425723 373 6 at at IN 10_1101-2021_01_07_425723 373 7 -80 -80 NNP 10_1101-2021_01_07_425723 373 8 ° ° NNP 10_1101-2021_01_07_425723 373 9 C C NNP 10_1101-2021_01_07_425723 373 10 and and CC 10_1101-2021_01_07_425723 373 11 processed process VBD 10_1101-2021_01_07_425723 373 12 within within IN 10_1101-2021_01_07_425723 373 13 10 10 CD 10_1101-2021_01_07_425723 373 14 days day NNS 10_1101-2021_01_07_425723 373 15 after after IN 10_1101-2021_01_07_425723 373 16 sampling sampling NN 10_1101-2021_01_07_425723 373 17 . . . 10_1101-2021_01_07_425723 374 1 After after IN 10_1101-2021_01_07_425723 374 2 569 569 CD 10_1101-2021_01_07_425723 374 3 thawing thaw VBG 10_1101-2021_01_07_425723 374 4 on on IN 10_1101-2021_01_07_425723 374 5 ice ice NN 10_1101-2021_01_07_425723 374 6 , , , 10_1101-2021_01_07_425723 374 7 cells cell NNS 10_1101-2021_01_07_425723 374 8 were be VBD 10_1101-2021_01_07_425723 374 9 harvested harvest VBN 10_1101-2021_01_07_425723 374 10 by by IN 10_1101-2021_01_07_425723 374 11 centrifugation centrifugation NN 10_1101-2021_01_07_425723 374 12 . . . 10_1101-2021_01_07_425723 375 1 Total Total NNP 10_1101-2021_01_07_425723 375 2 RNA RNA NNP 10_1101-2021_01_07_425723 375 3 was be VBD 10_1101-2021_01_07_425723 375 4 extracted extract VBN 10_1101-2021_01_07_425723 375 5 by by IN 10_1101-2021_01_07_425723 375 6 a a DT 10_1101-2021_01_07_425723 375 7 5 5 CD 10_1101-2021_01_07_425723 375 8 min min NN 10_1101-2021_01_07_425723 375 9 heatshock heatshock NN 10_1101-2021_01_07_425723 375 10 at at IN 10_1101-2021_01_07_425723 375 11 570 570 CD 10_1101-2021_01_07_425723 375 12 65 65 CD 10_1101-2021_01_07_425723 375 13 ° ° , 10_1101-2021_01_07_425723 375 14 C C NNP 10_1101-2021_01_07_425723 375 15 with with IN 10_1101-2021_01_07_425723 375 16 a a DT 10_1101-2021_01_07_425723 375 17 mix mix NN 10_1101-2021_01_07_425723 375 18 of of IN 10_1101-2021_01_07_425723 375 19 isoamyl isoamyl NNP 10_1101-2021_01_07_425723 375 20 alcohol alcohol NNP 10_1101-2021_01_07_425723 375 21 , , , 10_1101-2021_01_07_425723 375 22 phenol phenol NN 10_1101-2021_01_07_425723 375 23 and and CC 10_1101-2021_01_07_425723 375 24 chloroform chloroform NN 10_1101-2021_01_07_425723 375 25 at at IN 10_1101-2021_01_07_425723 375 26 a a DT 10_1101-2021_01_07_425723 375 27 ratio ratio NN 10_1101-2021_01_07_425723 375 28 of of IN 10_1101-2021_01_07_425723 375 29 125:24:1 125:24:1 CD 10_1101-2021_01_07_425723 375 30 , , , 10_1101-2021_01_07_425723 375 31 respectively respectively RB 10_1101-2021_01_07_425723 375 32 571 571 CD 10_1101-2021_01_07_425723 375 33 ( ( -LRB- 10_1101-2021_01_07_425723 375 34 Invitrogen Invitrogen NNP 10_1101-2021_01_07_425723 375 35 ) ) -RRB- 10_1101-2021_01_07_425723 375 36 . . . 10_1101-2021_01_07_425723 376 1 RNA RNA NNP 10_1101-2021_01_07_425723 376 2 was be VBD 10_1101-2021_01_07_425723 376 3 extracted extract VBN 10_1101-2021_01_07_425723 376 4 from from IN 10_1101-2021_01_07_425723 376 5 the the DT 10_1101-2021_01_07_425723 376 6 organic organic JJ 10_1101-2021_01_07_425723 376 7 phase phase NN 10_1101-2021_01_07_425723 376 8 with with IN 10_1101-2021_01_07_425723 376 9 Tris Tris NNP 10_1101-2021_01_07_425723 376 10 - - HYPH 10_1101-2021_01_07_425723 376 11 HCl HCl NNP 10_1101-2021_01_07_425723 376 12 and and CC 10_1101-2021_01_07_425723 376 13 subsequently subsequently RB 10_1101-2021_01_07_425723 376 14 precipitated precipitate VBN 10_1101-2021_01_07_425723 376 15 by by IN 10_1101-2021_01_07_425723 376 16 572 572 CD 10_1101-2021_01_07_425723 376 17 the the DT 10_1101-2021_01_07_425723 376 18 addition addition NN 10_1101-2021_01_07_425723 376 19 of of IN 10_1101-2021_01_07_425723 376 20 3 3 CD 10_1101-2021_01_07_425723 376 21 M M NNP 10_1101-2021_01_07_425723 376 22 Nac Nac NNP 10_1101-2021_01_07_425723 376 23 - - HYPH 10_1101-2021_01_07_425723 376 24 acetate acetate NNP 10_1101-2021_01_07_425723 376 25 and and CC 10_1101-2021_01_07_425723 376 26 40 40 CD 10_1101-2021_01_07_425723 376 27 % % NN 10_1101-2021_01_07_425723 376 28 ( ( -LRB- 10_1101-2021_01_07_425723 376 29 v v NN 10_1101-2021_01_07_425723 376 30 / / SYM 10_1101-2021_01_07_425723 376 31 v v NN 10_1101-2021_01_07_425723 376 32 ) ) -RRB- 10_1101-2021_01_07_425723 376 33 ethanol ethanol NN 10_1101-2021_01_07_425723 376 34 at at IN 10_1101-2021_01_07_425723 376 35 -20 -20 NNP 10_1101-2021_01_07_425723 376 36 ° ° NNP 10_1101-2021_01_07_425723 376 37 C C NNP 10_1101-2021_01_07_425723 376 38 . . . 10_1101-2021_01_07_425723 377 1 Precipitated Precipitated NNP 10_1101-2021_01_07_425723 377 2 RNA RNA NNP 10_1101-2021_01_07_425723 377 3 was be VBD 10_1101-2021_01_07_425723 377 4 washed wash VBN 10_1101-2021_01_07_425723 377 5 with with IN 10_1101-2021_01_07_425723 377 6 573 573 CD 10_1101-2021_01_07_425723 377 7 ethanol ethanol NNS 10_1101-2021_01_07_425723 377 8 , , , 10_1101-2021_01_07_425723 377 9 collected collect VBN 10_1101-2021_01_07_425723 377 10 and and CC 10_1101-2021_01_07_425723 377 11 after after IN 10_1101-2021_01_07_425723 377 12 drying drying NN 10_1101-2021_01_07_425723 377 13 resuspended resuspend VBD 10_1101-2021_01_07_425723 377 14 in in IN 10_1101-2021_01_07_425723 377 15 RNAse RNAse NNP 10_1101-2021_01_07_425723 377 16 free free JJ 10_1101-2021_01_07_425723 377 17 water water NN 10_1101-2021_01_07_425723 377 18 . . . 10_1101-2021_01_07_425723 378 1 The the DT 10_1101-2021_01_07_425723 378 2 quantity quantity NN 10_1101-2021_01_07_425723 378 3 of of IN 10_1101-2021_01_07_425723 378 4 total total NN 10_1101-2021_01_07_425723 378 5 RNA RNA NNP 10_1101-2021_01_07_425723 378 6 was be VBD 10_1101-2021_01_07_425723 378 7 574 574 CD 10_1101-2021_01_07_425723 378 8 determined determine VBN 10_1101-2021_01_07_425723 378 9 with with IN 10_1101-2021_01_07_425723 378 10 a a DT 10_1101-2021_01_07_425723 378 11 Qubit Qubit NNP 10_1101-2021_01_07_425723 378 12 RNA RNA NNP 10_1101-2021_01_07_425723 378 13 BR BR NNP 10_1101-2021_01_07_425723 378 14 assay assay NN 10_1101-2021_01_07_425723 378 15 kit kit NN 10_1101-2021_01_07_425723 378 16 ( ( -LRB- 10_1101-2021_01_07_425723 378 17 Thermo Thermo NNP 10_1101-2021_01_07_425723 378 18 Fisher Fisher NNP 10_1101-2021_01_07_425723 378 19 Scientific Scientific NNP 10_1101-2021_01_07_425723 378 20 ) ) -RRB- 10_1101-2021_01_07_425723 378 21 . . . 10_1101-2021_01_07_425723 379 1 RNA RNA NNP 10_1101-2021_01_07_425723 379 2 quality quality NN 10_1101-2021_01_07_425723 379 3 was be VBD 10_1101-2021_01_07_425723 379 4 determined determine VBN 10_1101-2021_01_07_425723 379 5 by by IN 10_1101-2021_01_07_425723 379 6 the the DT 10_1101-2021_01_07_425723 379 7 575 575 CD 10_1101-2021_01_07_425723 379 8 RNA RNA NNP 10_1101-2021_01_07_425723 379 9 integrity integrity NN 10_1101-2021_01_07_425723 379 10 number number NN 10_1101-2021_01_07_425723 379 11 with with IN 10_1101-2021_01_07_425723 379 12 RNA RNA NNP 10_1101-2021_01_07_425723 379 13 screen screen NN 10_1101-2021_01_07_425723 379 14 tape tape NN 10_1101-2021_01_07_425723 379 15 using use VBG 10_1101-2021_01_07_425723 379 16 a a DT 10_1101-2021_01_07_425723 379 17 Tapestation Tapestation NNP 10_1101-2021_01_07_425723 379 18 ( ( -LRB- 10_1101-2021_01_07_425723 379 19 Agilent Agilent NNP 10_1101-2021_01_07_425723 379 20 ) ) -RRB- 10_1101-2021_01_07_425723 379 21 . . . 10_1101-2021_01_07_425723 380 1 RNA RNA NNP 10_1101-2021_01_07_425723 380 2 libraries library NNS 10_1101-2021_01_07_425723 380 3 were be VBD 10_1101-2021_01_07_425723 380 4 prepared prepare VBN 10_1101-2021_01_07_425723 380 5 576 576 CD 10_1101-2021_01_07_425723 380 6 with with IN 10_1101-2021_01_07_425723 380 7 the the DT 10_1101-2021_01_07_425723 380 8 TruSeq TruSeq NNP 10_1101-2021_01_07_425723 380 9 Stranded Stranded NNP 10_1101-2021_01_07_425723 380 10 mRNA mrna NN 10_1101-2021_01_07_425723 380 11 LT LT NNP 10_1101-2021_01_07_425723 380 12 protocol protocol NNP 10_1101-2021_01_07_425723 380 13 ( ( -LRB- 10_1101-2021_01_07_425723 380 14 Illumina Illumina NNP 10_1101-2021_01_07_425723 380 15 , , , 10_1101-2021_01_07_425723 380 16 # # $ 10_1101-2021_01_07_425723 380 17 15031047 15031047 CD 10_1101-2021_01_07_425723 380 18 ) ) -RRB- 10_1101-2021_01_07_425723 380 19 and and CC 10_1101-2021_01_07_425723 380 20 subjected subject VBN 10_1101-2021_01_07_425723 380 21 to to IN 10_1101-2021_01_07_425723 380 22 paired pair VBN 10_1101-2021_01_07_425723 380 23 - - HYPH 10_1101-2021_01_07_425723 380 24 end end VB 10_1101-2021_01_07_425723 380 25 577 577 CD 10_1101-2021_01_07_425723 380 26 sequencing sequencing NN 10_1101-2021_01_07_425723 380 27 ( ( -LRB- 10_1101-2021_01_07_425723 380 28 151 151 CD 10_1101-2021_01_07_425723 380 29 bp bp NN 10_1101-2021_01_07_425723 380 30 read read VB 10_1101-2021_01_07_425723 380 31 length length NN 10_1101-2021_01_07_425723 380 32 , , , 10_1101-2021_01_07_425723 380 33 NovaSeq NovaSeq NNP 10_1101-2021_01_07_425723 380 34 Illumina Illumina NNP 10_1101-2021_01_07_425723 380 35 ) ) -RRB- 10_1101-2021_01_07_425723 380 36 by by IN 10_1101-2021_01_07_425723 380 37 Macrogen Macrogen NNP 10_1101-2021_01_07_425723 380 38 ( ( -LRB- 10_1101-2021_01_07_425723 380 39 Macrogen Macrogen NNP 10_1101-2021_01_07_425723 380 40 Europe Europe NNP 10_1101-2021_01_07_425723 380 41 , , , 10_1101-2021_01_07_425723 380 42 Amsterdam Amsterdam NNP 10_1101-2021_01_07_425723 380 43 , , , 10_1101-2021_01_07_425723 380 44 the the DT 10_1101-2021_01_07_425723 380 45 578 578 CD 10_1101-2021_01_07_425723 380 46 Netherlands Netherlands NNP 10_1101-2021_01_07_425723 380 47 ) ) -RRB- 10_1101-2021_01_07_425723 380 48 . . . 10_1101-2021_01_07_425723 381 1 579 579 CD 10_1101-2021_01_07_425723 381 2 Pooled pool VBN 10_1101-2021_01_07_425723 381 3 RNAseq RNAseq NNP 10_1101-2021_01_07_425723 381 4 libraries library NNS 10_1101-2021_01_07_425723 381 5 were be VBD 10_1101-2021_01_07_425723 381 6 used use VBN 10_1101-2021_01_07_425723 381 7 to to TO 10_1101-2021_01_07_425723 381 8 perform perform VB 10_1101-2021_01_07_425723 381 9 de de FW 10_1101-2021_01_07_425723 381 10 novo novo NNP 10_1101-2021_01_07_425723 381 11 transcriptome transcriptome NNP 10_1101-2021_01_07_425723 381 12 assembly assembly NN 10_1101-2021_01_07_425723 381 13 using use VBG 10_1101-2021_01_07_425723 381 14 Trinity Trinity NNP 10_1101-2021_01_07_425723 381 15 ( ( -LRB- 10_1101-2021_01_07_425723 381 16 v2.8.3)75 v2.8.3)75 NNP 10_1101-2021_01_07_425723 381 17 580 580 CD 10_1101-2021_01_07_425723 381 18 which which WDT 10_1101-2021_01_07_425723 381 19 was be VBD 10_1101-2021_01_07_425723 381 20 subsequently subsequently RB 10_1101-2021_01_07_425723 381 21 used use VBN 10_1101-2021_01_07_425723 381 22 as as IN 10_1101-2021_01_07_425723 381 23 evidence evidence NN 10_1101-2021_01_07_425723 381 24 for for IN 10_1101-2021_01_07_425723 381 25 both both CC 10_1101-2021_01_07_425723 381 26 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 381 27 and and CC 10_1101-2021_01_07_425723 381 28 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 381 29 genome genome JJ 10_1101-2021_01_07_425723 381 30 annotations annotation NNS 10_1101-2021_01_07_425723 381 31 . . . 10_1101-2021_01_07_425723 382 1 581 581 CD 10_1101-2021_01_07_425723 382 2 RNAseq RNAseq NNP 10_1101-2021_01_07_425723 382 3 libraries library NNS 10_1101-2021_01_07_425723 382 4 were be VBD 10_1101-2021_01_07_425723 382 5 mapped map VBN 10_1101-2021_01_07_425723 382 6 into into IN 10_1101-2021_01_07_425723 382 7 the the DT 10_1101-2021_01_07_425723 382 8 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 382 9 genome genome NNP 10_1101-2021_01_07_425723 382 10 assembly assembly NNP 10_1101-2021_01_07_425723 382 11 described describe VBN 10_1101-2021_01_07_425723 382 12 above above RB 10_1101-2021_01_07_425723 382 13 , , , 10_1101-2021_01_07_425723 382 14 using use VBG 10_1101-2021_01_07_425723 382 15 bowtie bowtie NN 10_1101-2021_01_07_425723 382 16 582 582 CD 10_1101-2021_01_07_425723 382 17 ( ( -LRB- 10_1101-2021_01_07_425723 382 18 v1.2.1.1)76 v1.2.1.1)76 NN 10_1101-2021_01_07_425723 382 19 with with IN 10_1101-2021_01_07_425723 382 20 parameters parameter NNS 10_1101-2021_01_07_425723 382 21 ( ( -LRB- 10_1101-2021_01_07_425723 382 22 -v -v NFP 10_1101-2021_01_07_425723 382 23 0 0 CD 10_1101-2021_01_07_425723 382 24 -k -k SYM 10_1101-2021_01_07_425723 382 25 10 10 CD 10_1101-2021_01_07_425723 382 26 --best --best JJ 10_1101-2021_01_07_425723 382 27 -M -M : 10_1101-2021_01_07_425723 382 28 1 1 CD 10_1101-2021_01_07_425723 382 29 ) ) -RRB- 10_1101-2021_01_07_425723 382 30 to to TO 10_1101-2021_01_07_425723 382 31 allow allow VB 10_1101-2021_01_07_425723 382 32 no no DT 10_1101-2021_01_07_425723 382 33 mismatches mismatch NNS 10_1101-2021_01_07_425723 382 34 , , , 10_1101-2021_01_07_425723 382 35 select select VB 10_1101-2021_01_07_425723 382 36 the the DT 10_1101-2021_01_07_425723 382 37 best good JJS 10_1101-2021_01_07_425723 382 38 out out IN 10_1101-2021_01_07_425723 382 39 of of IN 10_1101-2021_01_07_425723 382 40 10 10 CD 10_1101-2021_01_07_425723 382 41 583 583 CD 10_1101-2021_01_07_425723 382 42 possible possible JJ 10_1101-2021_01_07_425723 382 43 alignments alignment NNS 10_1101-2021_01_07_425723 382 44 per per IN 10_1101-2021_01_07_425723 382 45 read read NN 10_1101-2021_01_07_425723 382 46 , , , 10_1101-2021_01_07_425723 382 47 and and CC 10_1101-2021_01_07_425723 382 48 for for IN 10_1101-2021_01_07_425723 382 49 reads read NNS 10_1101-2021_01_07_425723 382 50 having have VBG 10_1101-2021_01_07_425723 382 51 more more JJR 10_1101-2021_01_07_425723 382 52 than than IN 10_1101-2021_01_07_425723 382 53 one one CD 10_1101-2021_01_07_425723 382 54 possible possible JJ 10_1101-2021_01_07_425723 382 55 alignment alignment NN 10_1101-2021_01_07_425723 382 56 randomly randomly RB 10_1101-2021_01_07_425723 382 57 report report VB 10_1101-2021_01_07_425723 382 58 584 584 CD 10_1101-2021_01_07_425723 382 59 only only RB 10_1101-2021_01_07_425723 382 60 one one CD 10_1101-2021_01_07_425723 382 61 . . . 10_1101-2021_01_07_425723 383 1 Alignments alignment NNS 10_1101-2021_01_07_425723 383 2 were be VBD 10_1101-2021_01_07_425723 383 3 filtered filter VBN 10_1101-2021_01_07_425723 383 4 and and CC 10_1101-2021_01_07_425723 383 5 sorted sort VBN 10_1101-2021_01_07_425723 383 6 using use VBG 10_1101-2021_01_07_425723 383 7 samtools samtool NNS 10_1101-2021_01_07_425723 383 8 ( ( -LRB- 10_1101-2021_01_07_425723 383 9 v1.3.1)77 v1.3.1)77 NNP 10_1101-2021_01_07_425723 383 10 . . . 10_1101-2021_01_07_425723 384 1 Read Read VBN 10_1101-2021_01_07_425723 384 2 counts count NNS 10_1101-2021_01_07_425723 384 3 were be VBD 10_1101-2021_01_07_425723 384 4 obtained obtain VBN 10_1101-2021_01_07_425723 384 5 585 585 CD 10_1101-2021_01_07_425723 384 6 .CC .CC : 10_1101-2021_01_07_425723 384 7 - - HYPH 10_1101-2021_01_07_425723 384 8 BY by IN 10_1101-2021_01_07_425723 384 9 - - HYPH 10_1101-2021_01_07_425723 384 10 NC NC NNP 10_1101-2021_01_07_425723 384 11 - - HYPH 10_1101-2021_01_07_425723 384 12 ND ND NNP 10_1101-2021_01_07_425723 384 13 4.0 4.0 CD 10_1101-2021_01_07_425723 384 14 International International NNP 10_1101-2021_01_07_425723 384 15 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 384 16 under under IN 10_1101-2021_01_07_425723 384 17 a a DT 10_1101-2021_01_07_425723 384 18 ( ( -LRB- 10_1101-2021_01_07_425723 384 19 which which WDT 10_1101-2021_01_07_425723 384 20 was be VBD 10_1101-2021_01_07_425723 384 21 not not RB 10_1101-2021_01_07_425723 384 22 certified certify VBN 10_1101-2021_01_07_425723 384 23 by by IN 10_1101-2021_01_07_425723 384 24 peer peer NN 10_1101-2021_01_07_425723 384 25 review review NN 10_1101-2021_01_07_425723 384 26 ) ) -RRB- 10_1101-2021_01_07_425723 384 27 is be VBZ 10_1101-2021_01_07_425723 384 28 the the DT 10_1101-2021_01_07_425723 384 29 author author NN 10_1101-2021_01_07_425723 384 30 / / SYM 10_1101-2021_01_07_425723 384 31 funder funder NN 10_1101-2021_01_07_425723 384 32 , , , 10_1101-2021_01_07_425723 384 33 who who WP 10_1101-2021_01_07_425723 384 34 has have VBZ 10_1101-2021_01_07_425723 384 35 granted grant VBN 10_1101-2021_01_07_425723 384 36 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 384 37 a a DT 10_1101-2021_01_07_425723 384 38 license license NN 10_1101-2021_01_07_425723 384 39 to to TO 10_1101-2021_01_07_425723 384 40 display display VB 10_1101-2021_01_07_425723 384 41 the the DT 10_1101-2021_01_07_425723 384 42 preprint preprint NN 10_1101-2021_01_07_425723 384 43 in in IN 10_1101-2021_01_07_425723 384 44 perpetuity perpetuity NN 10_1101-2021_01_07_425723 384 45 . . . 10_1101-2021_01_07_425723 385 1 It -PRON- PRP 10_1101-2021_01_07_425723 385 2 is be VBZ 10_1101-2021_01_07_425723 385 3 made make VBN 10_1101-2021_01_07_425723 385 4 The the DT 10_1101-2021_01_07_425723 385 5 copyright copyright NN 10_1101-2021_01_07_425723 385 6 holder holder NN 10_1101-2021_01_07_425723 385 7 for for IN 10_1101-2021_01_07_425723 385 8 this this DT 10_1101-2021_01_07_425723 385 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 385 10 version version NN 10_1101-2021_01_07_425723 385 11 posted post VBD 10_1101-2021_01_07_425723 385 12 January January NNP 10_1101-2021_01_07_425723 385 13 8 8 CD 10_1101-2021_01_07_425723 385 14 , , , 10_1101-2021_01_07_425723 385 15 2021 2021 CD 10_1101-2021_01_07_425723 385 16 . . . 10_1101-2021_01_07_425723 385 17 ; ; : 10_1101-2021_01_07_425723 385 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 385 19 : : : 10_1101-2021_01_07_425723 385 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 385 21 preprint preprint NN 10_1101-2021_01_07_425723 385 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 385 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425723 385 24 34 34 CD 10_1101-2021_01_07_425723 385 25 with with IN 10_1101-2021_01_07_425723 385 26 featureCounts featurecount NNS 10_1101-2021_01_07_425723 385 27 ( ( -LRB- 10_1101-2021_01_07_425723 385 28 v1.6.0)78 v1.6.0)78 NN 10_1101-2021_01_07_425723 385 29 using use VBG 10_1101-2021_01_07_425723 385 30 parameters parameter NNS 10_1101-2021_01_07_425723 385 31 ( ( -LRB- 10_1101-2021_01_07_425723 385 32 -B -B : 10_1101-2021_01_07_425723 385 33 -C -C NFP 10_1101-2021_01_07_425723 385 34 ) ) -RRB- 10_1101-2021_01_07_425723 385 35 to to TO 10_1101-2021_01_07_425723 385 36 only only RB 10_1101-2021_01_07_425723 385 37 count count VB 10_1101-2021_01_07_425723 385 38 reads read VBZ 10_1101-2021_01_07_425723 385 39 for for IN 10_1101-2021_01_07_425723 385 40 which which WDT 10_1101-2021_01_07_425723 385 41 both both DT 10_1101-2021_01_07_425723 385 42 pairs pair NNS 10_1101-2021_01_07_425723 385 43 are be VBP 10_1101-2021_01_07_425723 385 44 586 586 CD 10_1101-2021_01_07_425723 385 45 aligned align VBN 10_1101-2021_01_07_425723 385 46 into into IN 10_1101-2021_01_07_425723 385 47 the the DT 10_1101-2021_01_07_425723 385 48 same same JJ 10_1101-2021_01_07_425723 385 49 chromosome chromosome NN 10_1101-2021_01_07_425723 385 50 . . . 10_1101-2021_01_07_425723 386 1 587 587 CD 10_1101-2021_01_07_425723 386 2 Differential differential NN 10_1101-2021_01_07_425723 386 3 gene gene NN 10_1101-2021_01_07_425723 386 4 expression expression NN 10_1101-2021_01_07_425723 386 5 ( ( -LRB- 10_1101-2021_01_07_425723 386 6 DGE DGE NNP 10_1101-2021_01_07_425723 386 7 ) ) -RRB- 10_1101-2021_01_07_425723 386 8 analysis analysis NN 10_1101-2021_01_07_425723 386 9 was be VBD 10_1101-2021_01_07_425723 386 10 performed perform VBN 10_1101-2021_01_07_425723 386 11 using use VBG 10_1101-2021_01_07_425723 386 12 edgeR edgeR NNP 10_1101-2021_01_07_425723 386 13 ( ( -LRB- 10_1101-2021_01_07_425723 386 14 v3.28.1)79 v3.28.1)79 CD 10_1101-2021_01_07_425723 386 15 . . . 10_1101-2021_01_07_425723 387 1 Genes gene NNS 10_1101-2021_01_07_425723 387 2 with with IN 10_1101-2021_01_07_425723 387 3 0 0 CD 10_1101-2021_01_07_425723 387 4 read read NN 10_1101-2021_01_07_425723 387 5 588 588 CD 10_1101-2021_01_07_425723 387 6 counts count NNS 10_1101-2021_01_07_425723 387 7 in in IN 10_1101-2021_01_07_425723 387 8 all all DT 10_1101-2021_01_07_425723 387 9 conditions condition NNS 10_1101-2021_01_07_425723 387 10 were be VBD 10_1101-2021_01_07_425723 387 11 filtered filter VBN 10_1101-2021_01_07_425723 387 12 out out RP 10_1101-2021_01_07_425723 387 13 from from IN 10_1101-2021_01_07_425723 387 14 the the DT 10_1101-2021_01_07_425723 387 15 analysis analysis NN 10_1101-2021_01_07_425723 387 16 , , , 10_1101-2021_01_07_425723 387 17 same same JJ 10_1101-2021_01_07_425723 387 18 as as IN 10_1101-2021_01_07_425723 387 19 genes gene NNS 10_1101-2021_01_07_425723 387 20 with with IN 10_1101-2021_01_07_425723 387 21 less less JJR 10_1101-2021_01_07_425723 387 22 than than IN 10_1101-2021_01_07_425723 387 23 10 10 CD 10_1101-2021_01_07_425723 387 24 counts count NNS 10_1101-2021_01_07_425723 387 25 per per IN 10_1101-2021_01_07_425723 387 26 589 589 CD 10_1101-2021_01_07_425723 387 27 million million CD 10_1101-2021_01_07_425723 387 28 . . . 10_1101-2021_01_07_425723 388 1 Counts count NNS 10_1101-2021_01_07_425723 388 2 were be VBD 10_1101-2021_01_07_425723 388 3 normalized normalized JJ 10_1101-2021_01_07_425723 388 4 using use VBG 10_1101-2021_01_07_425723 388 5 the the DT 10_1101-2021_01_07_425723 388 6 trimmed trim VBN 10_1101-2021_01_07_425723 388 7 mean mean NN 10_1101-2021_01_07_425723 388 8 of of IN 10_1101-2021_01_07_425723 388 9 M M NNP 10_1101-2021_01_07_425723 388 10 values value NNS 10_1101-2021_01_07_425723 388 11 ( ( -LRB- 10_1101-2021_01_07_425723 388 12 TMM TMM NNP 10_1101-2021_01_07_425723 388 13 ) ) -RRB- 10_1101-2021_01_07_425723 388 14 method80 method80 NNP 10_1101-2021_01_07_425723 388 15 , , , 10_1101-2021_01_07_425723 388 16 and and CC 10_1101-2021_01_07_425723 388 17 dispersion dispersion NN 10_1101-2021_01_07_425723 388 18 590 590 CD 10_1101-2021_01_07_425723 388 19 was be VBD 10_1101-2021_01_07_425723 388 20 estimated estimate VBN 10_1101-2021_01_07_425723 388 21 using use VBG 10_1101-2021_01_07_425723 388 22 generalized generalize VBN 10_1101-2021_01_07_425723 388 23 linear linear JJ 10_1101-2021_01_07_425723 388 24 models model NNS 10_1101-2021_01_07_425723 388 25 . . . 10_1101-2021_01_07_425723 389 1 Differentially differentially RB 10_1101-2021_01_07_425723 389 2 expressed express VBD 10_1101-2021_01_07_425723 389 3 genes gene NNS 10_1101-2021_01_07_425723 389 4 were be VBD 10_1101-2021_01_07_425723 389 5 then then RB 10_1101-2021_01_07_425723 389 6 calculated calculate VBN 10_1101-2021_01_07_425723 389 7 591 591 CD 10_1101-2021_01_07_425723 389 8 using use VBG 10_1101-2021_01_07_425723 389 9 a a DT 10_1101-2021_01_07_425723 389 10 log log NN 10_1101-2021_01_07_425723 389 11 ratio ratio NN 10_1101-2021_01_07_425723 389 12 test test NN 10_1101-2021_01_07_425723 389 13 adjusted adjust VBN 10_1101-2021_01_07_425723 389 14 with with IN 10_1101-2021_01_07_425723 389 15 the the DT 10_1101-2021_01_07_425723 389 16 Benjamini Benjamini NNP 10_1101-2021_01_07_425723 389 17 - - HYPH 10_1101-2021_01_07_425723 389 18 Hochberg Hochberg NNP 10_1101-2021_01_07_425723 389 19 method method NN 10_1101-2021_01_07_425723 389 20 . . . 10_1101-2021_01_07_425723 390 1 Absolute absolute JJ 10_1101-2021_01_07_425723 390 2 log2 log2 NN 10_1101-2021_01_07_425723 390 3 fold fold JJ 10_1101-2021_01_07_425723 390 4 - - HYPH 10_1101-2021_01_07_425723 390 5 change change NN 10_1101-2021_01_07_425723 390 6 values value NNS 10_1101-2021_01_07_425723 390 7 > > . 10_1101-2021_01_07_425723 390 8 592 592 CD 10_1101-2021_01_07_425723 390 9 2 2 CD 10_1101-2021_01_07_425723 390 10 , , , 10_1101-2021_01_07_425723 390 11 false false JJ 10_1101-2021_01_07_425723 390 12 discovery discovery NN 10_1101-2021_01_07_425723 390 13 rate rate NN 10_1101-2021_01_07_425723 390 14 < < XX 10_1101-2021_01_07_425723 390 15 0.5 0.5 CD 10_1101-2021_01_07_425723 390 16 , , , 10_1101-2021_01_07_425723 390 17 and and CC 10_1101-2021_01_07_425723 390 18 P p NN 10_1101-2021_01_07_425723 390 19 value value NN 10_1101-2021_01_07_425723 390 20 < < XX 10_1101-2021_01_07_425723 390 21 0.05 0.05 CD 10_1101-2021_01_07_425723 390 22 were be VBD 10_1101-2021_01_07_425723 390 23 used use VBN 10_1101-2021_01_07_425723 390 24 as as IN 10_1101-2021_01_07_425723 390 25 significance significance NN 10_1101-2021_01_07_425723 390 26 cutoffs cutoff NNS 10_1101-2021_01_07_425723 390 27 . . . 10_1101-2021_01_07_425723 391 1 593 593 CD 10_1101-2021_01_07_425723 391 2 Gene gene NN 10_1101-2021_01_07_425723 391 3 set set NN 10_1101-2021_01_07_425723 391 4 analysis analysis NN 10_1101-2021_01_07_425723 391 5 ( ( -LRB- 10_1101-2021_01_07_425723 391 6 GSA GSA NNP 10_1101-2021_01_07_425723 391 7 ) ) -RRB- 10_1101-2021_01_07_425723 391 8 based base VBN 10_1101-2021_01_07_425723 391 9 on on IN 10_1101-2021_01_07_425723 391 10 gene gene NN 10_1101-2021_01_07_425723 391 11 ontology ontology NN 10_1101-2021_01_07_425723 391 12 ( ( -LRB- 10_1101-2021_01_07_425723 391 13 GO GO NNP 10_1101-2021_01_07_425723 391 14 ) ) -RRB- 10_1101-2021_01_07_425723 391 15 terms term NNS 10_1101-2021_01_07_425723 391 16 was be VBD 10_1101-2021_01_07_425723 391 17 used use VBN 10_1101-2021_01_07_425723 391 18 to to TO 10_1101-2021_01_07_425723 391 19 get get VB 10_1101-2021_01_07_425723 391 20 a a DT 10_1101-2021_01_07_425723 391 21 functional functional JJ 10_1101-2021_01_07_425723 391 22 interpretation interpretation NN 10_1101-2021_01_07_425723 391 23 594 594 CD 10_1101-2021_01_07_425723 391 24 of of IN 10_1101-2021_01_07_425723 391 25 the the DT 10_1101-2021_01_07_425723 391 26 DGE DGE NNP 10_1101-2021_01_07_425723 391 27 analysis analysis NN 10_1101-2021_01_07_425723 391 28 . . . 10_1101-2021_01_07_425723 392 1 For for IN 10_1101-2021_01_07_425723 392 2 this this DT 10_1101-2021_01_07_425723 392 3 purpose purpose NN 10_1101-2021_01_07_425723 392 4 , , , 10_1101-2021_01_07_425723 392 5 GO GO NNP 10_1101-2021_01_07_425723 392 6 terms term NNS 10_1101-2021_01_07_425723 392 7 were be VBD 10_1101-2021_01_07_425723 392 8 first first RB 10_1101-2021_01_07_425723 392 9 obtained obtain VBN 10_1101-2021_01_07_425723 392 10 for for IN 10_1101-2021_01_07_425723 392 11 the the DT 10_1101-2021_01_07_425723 392 12 S. S. NNP 10_1101-2021_01_07_425723 392 13 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 392 14 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 392 15 - - HYPH 10_1101-2021_01_07_425723 392 16 7D 7D NNP 10_1101-2021_01_07_425723 392 17 595 595 CD 10_1101-2021_01_07_425723 392 18 ( ( -LRB- 10_1101-2021_01_07_425723 392 19 GCA_002571405.2 GCA_002571405.2 NNP 10_1101-2021_01_07_425723 392 20 ) ) -RRB- 10_1101-2021_01_07_425723 392 21 and and CC 10_1101-2021_01_07_425723 392 22 K. K. NNP 10_1101-2021_01_07_425723 392 23 marxianus marxianus NN 10_1101-2021_01_07_425723 392 24 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 392 25 genome genome NNP 10_1101-2021_01_07_425723 392 26 annotations annotation NNS 10_1101-2021_01_07_425723 392 27 using use VBG 10_1101-2021_01_07_425723 392 28 Funannotate Funannotate NNP 10_1101-2021_01_07_425723 392 29 and and CC 10_1101-2021_01_07_425723 392 30 596 596 CD 10_1101-2021_01_07_425723 392 31 Interproscan Interproscan NNPS 10_1101-2021_01_07_425723 392 32 as as IN 10_1101-2021_01_07_425723 392 33 described describe VBN 10_1101-2021_01_07_425723 392 34 above above RB 10_1101-2021_01_07_425723 392 35 . . . 10_1101-2021_01_07_425723 393 1 Afterwards afterwards RB 10_1101-2021_01_07_425723 393 2 , , , 10_1101-2021_01_07_425723 393 3 Funannotate Funannotate NNP 10_1101-2021_01_07_425723 393 4 compare compare NN 10_1101-2021_01_07_425723 393 5 was be VBD 10_1101-2021_01_07_425723 393 6 used use VBN 10_1101-2021_01_07_425723 393 7 to to TO 10_1101-2021_01_07_425723 393 8 get get VB 10_1101-2021_01_07_425723 393 9 ( ( -LRB- 10_1101-2021_01_07_425723 393 10 co)ortholog co)ortholog NN 10_1101-2021_01_07_425723 393 11 597 597 CD 10_1101-2021_01_07_425723 393 12 groups group NNS 10_1101-2021_01_07_425723 393 13 of of IN 10_1101-2021_01_07_425723 393 14 genes gene NNS 10_1101-2021_01_07_425723 393 15 generated generate VBN 10_1101-2021_01_07_425723 393 16 with with IN 10_1101-2021_01_07_425723 393 17 ProteinOrtho538 ProteinOrtho538 NNP 10_1101-2021_01_07_425723 393 18 using use VBG 10_1101-2021_01_07_425723 393 19 the the DT 10_1101-2021_01_07_425723 393 20 following follow VBG 10_1101-2021_01_07_425723 393 21 public public JJ 10_1101-2021_01_07_425723 393 22 genome genome JJ 10_1101-2021_01_07_425723 393 23 annotations annotation NNS 10_1101-2021_01_07_425723 393 24 S. S. NNP 10_1101-2021_01_07_425723 393 25 598 598 CD 10_1101-2021_01_07_425723 393 26 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 393 27 S288C s288c CD 10_1101-2021_01_07_425723 393 28 ( ( -LRB- 10_1101-2021_01_07_425723 393 29 GCF_000146045.2 GCF_000146045.2 NNP 10_1101-2021_01_07_425723 393 30 ) ) -RRB- 10_1101-2021_01_07_425723 393 31 , , , 10_1101-2021_01_07_425723 393 32 K. K. NNP 10_1101-2021_01_07_425723 393 33 marxianus marxianus NN 10_1101-2021_01_07_425723 393 34 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 393 35 ( ( -LRB- 10_1101-2021_01_07_425723 393 36 GCA_001417835.1 GCA_001417835.1 NNP 10_1101-2021_01_07_425723 393 37 ) ) -RRB- 10_1101-2021_01_07_425723 393 38 , , , 10_1101-2021_01_07_425723 393 39 K. K. NNP 10_1101-2021_01_07_425723 393 40 marxianus marxianus NNP 10_1101-2021_01_07_425723 393 41 599 599 CD 10_1101-2021_01_07_425723 393 42 DMKU3 DMKU3 NNP 10_1101-2021_01_07_425723 393 43 - - HYPH 10_1101-2021_01_07_425723 393 44 1042 1042 CD 10_1101-2021_01_07_425723 393 45 ( ( -LRB- 10_1101-2021_01_07_425723 393 46 GCF_001417885.1 GCF_001417885.1 NNP 10_1101-2021_01_07_425723 393 47 ) ) -RRB- 10_1101-2021_01_07_425723 393 48 , , , 10_1101-2021_01_07_425723 393 49 in in IN 10_1101-2021_01_07_425723 393 50 addition addition NN 10_1101-2021_01_07_425723 393 51 to to IN 10_1101-2021_01_07_425723 393 52 the the DT 10_1101-2021_01_07_425723 393 53 new new JJ 10_1101-2021_01_07_425723 393 54 genome genome JJ 10_1101-2021_01_07_425723 393 55 annotations annotation NNS 10_1101-2021_01_07_425723 393 56 generated generate VBN 10_1101-2021_01_07_425723 393 57 here here RB 10_1101-2021_01_07_425723 393 58 for for IN 10_1101-2021_01_07_425723 393 59 S. S. NNP 10_1101-2021_01_07_425723 393 60 600 600 CD 10_1101-2021_01_07_425723 393 61 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 393 62 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 393 63 - - HYPH 10_1101-2021_01_07_425723 393 64 7D 7D NNP 10_1101-2021_01_07_425723 393 65 , , , 10_1101-2021_01_07_425723 393 66 and and CC 10_1101-2021_01_07_425723 393 67 K. K. NNP 10_1101-2021_01_07_425723 393 68 marxianus marxianus NN 10_1101-2021_01_07_425723 393 69 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 393 70 and and CC 10_1101-2021_01_07_425723 393 71 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 393 72 . . . 10_1101-2021_01_07_425723 394 1 Predicted predict VBN 10_1101-2021_01_07_425723 394 2 GO GO NNP 10_1101-2021_01_07_425723 394 3 terms term NNS 10_1101-2021_01_07_425723 394 4 for for IN 10_1101-2021_01_07_425723 394 5 S. S. NNP 10_1101-2021_01_07_425723 394 6 601 601 CD 10_1101-2021_01_07_425723 394 7 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 394 8 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 394 9 - - HYPH 10_1101-2021_01_07_425723 394 10 7D 7D NNP 10_1101-2021_01_07_425723 394 11 and and CC 10_1101-2021_01_07_425723 394 12 K. K. NNP 10_1101-2021_01_07_425723 394 13 marxianus marxianus NN 10_1101-2021_01_07_425723 394 14 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 394 15 were be VBD 10_1101-2021_01_07_425723 394 16 kept keep VBN 10_1101-2021_01_07_425723 394 17 , , , 10_1101-2021_01_07_425723 394 18 and and CC 10_1101-2021_01_07_425723 394 19 merged merge VBN 10_1101-2021_01_07_425723 394 20 with with IN 10_1101-2021_01_07_425723 394 21 those those DT 10_1101-2021_01_07_425723 394 22 from from IN 10_1101-2021_01_07_425723 394 23 602 602 CD 10_1101-2021_01_07_425723 394 24 corresponding corresponding JJ 10_1101-2021_01_07_425723 394 25 ( ( -LRB- 10_1101-2021_01_07_425723 394 26 co)orthologs co)orthologs NNP 10_1101-2021_01_07_425723 394 27 from from IN 10_1101-2021_01_07_425723 394 28 S. S. NNP 10_1101-2021_01_07_425723 394 29 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 394 30 S288C. s288c. XX 10_1101-2021_01_07_425723 395 1 Genes gene NNS 10_1101-2021_01_07_425723 395 2 with with IN 10_1101-2021_01_07_425723 395 3 term term NN 10_1101-2021_01_07_425723 395 4 GO:0005840 GO:0005840 NNP 10_1101-2021_01_07_425723 395 5 ( ( -LRB- 10_1101-2021_01_07_425723 395 6 ribosome ribosome VBN 10_1101-2021_01_07_425723 395 7 ) ) -RRB- 10_1101-2021_01_07_425723 395 8 were be VBD 10_1101-2021_01_07_425723 395 9 603 603 CD 10_1101-2021_01_07_425723 395 10 not not RB 10_1101-2021_01_07_425723 395 11 considered consider VBN 10_1101-2021_01_07_425723 395 12 for for IN 10_1101-2021_01_07_425723 395 13 further further JJ 10_1101-2021_01_07_425723 395 14 analyses analysis NNS 10_1101-2021_01_07_425723 395 15 . . . 10_1101-2021_01_07_425723 396 1 GSA GSA NNP 10_1101-2021_01_07_425723 396 2 was be VBD 10_1101-2021_01_07_425723 396 3 then then RB 10_1101-2021_01_07_425723 396 4 performed perform VBN 10_1101-2021_01_07_425723 396 5 with with IN 10_1101-2021_01_07_425723 396 6 Piano Piano NNP 10_1101-2021_01_07_425723 396 7 ( ( -LRB- 10_1101-2021_01_07_425723 396 8 v2.4.0)40 v2.4.0)40 NN 10_1101-2021_01_07_425723 396 9 . . . 10_1101-2021_01_07_425723 397 1 Gene gene NN 10_1101-2021_01_07_425723 397 2 set set NN 10_1101-2021_01_07_425723 397 3 statistics statistic NNS 10_1101-2021_01_07_425723 397 4 604 604 CD 10_1101-2021_01_07_425723 397 5 were be VBD 10_1101-2021_01_07_425723 397 6 first first RB 10_1101-2021_01_07_425723 397 7 calculated calculate VBN 10_1101-2021_01_07_425723 397 8 with with IN 10_1101-2021_01_07_425723 397 9 the the DT 10_1101-2021_01_07_425723 397 10 Stouffer Stouffer NNP 10_1101-2021_01_07_425723 397 11 , , , 10_1101-2021_01_07_425723 397 12 Wilcoxon Wilcoxon NNP 10_1101-2021_01_07_425723 397 13 rank rank NN 10_1101-2021_01_07_425723 397 14 - - HYPH 10_1101-2021_01_07_425723 397 15 sum sum NN 10_1101-2021_01_07_425723 397 16 test test NN 10_1101-2021_01_07_425723 397 17 , , , 10_1101-2021_01_07_425723 397 18 and and CC 10_1101-2021_01_07_425723 397 19 reporter reporter NN 10_1101-2021_01_07_425723 397 20 methods method NNS 10_1101-2021_01_07_425723 397 21 implemented implement VBN 10_1101-2021_01_07_425723 397 22 in in IN 10_1101-2021_01_07_425723 397 23 605 605 CD 10_1101-2021_01_07_425723 397 24 Piano Piano NNP 10_1101-2021_01_07_425723 397 25 . . . 10_1101-2021_01_07_425723 398 1 Afterwards afterwards RB 10_1101-2021_01_07_425723 398 2 , , , 10_1101-2021_01_07_425723 398 3 consensus consensus NN 10_1101-2021_01_07_425723 398 4 results result NNS 10_1101-2021_01_07_425723 398 5 were be VBD 10_1101-2021_01_07_425723 398 6 derived derive VBN 10_1101-2021_01_07_425723 398 7 by by IN 10_1101-2021_01_07_425723 398 8 p p NN 10_1101-2021_01_07_425723 398 9 - - HYPH 10_1101-2021_01_07_425723 398 10 value value NN 10_1101-2021_01_07_425723 398 11 and and CC 10_1101-2021_01_07_425723 398 12 rank rank NN 10_1101-2021_01_07_425723 398 13 aggregation aggregation NN 10_1101-2021_01_07_425723 398 14 , , , 10_1101-2021_01_07_425723 398 15 considered consider VBD 10_1101-2021_01_07_425723 398 16 606 606 CD 10_1101-2021_01_07_425723 398 17 significant significant JJ 10_1101-2021_01_07_425723 398 18 if if IN 10_1101-2021_01_07_425723 398 19 absolute absolute JJ 10_1101-2021_01_07_425723 398 20 Fold Fold NNP 10_1101-2021_01_07_425723 398 21 Change Change NNP 10_1101-2021_01_07_425723 398 22 values value NNS 10_1101-2021_01_07_425723 398 23 > > XX 10_1101-2021_01_07_425723 398 24 1 1 CD 10_1101-2021_01_07_425723 398 25 . . . 10_1101-2021_01_07_425723 399 1 ComplexHeatmap ComplexHeatmap NNP 10_1101-2021_01_07_425723 399 2 ( ( -LRB- 10_1101-2021_01_07_425723 399 3 v2.4.3)81 v2.4.3)81 NNP 10_1101-2021_01_07_425723 399 4 was be VBD 10_1101-2021_01_07_425723 399 5 used use VBN 10_1101-2021_01_07_425723 399 6 to to TO 10_1101-2021_01_07_425723 399 7 draw draw VB 10_1101-2021_01_07_425723 399 8 GSA GSA NNP 10_1101-2021_01_07_425723 399 9 results result VBZ 10_1101-2021_01_07_425723 399 10 607 607 CD 10_1101-2021_01_07_425723 399 11 .CC .CC , 10_1101-2021_01_07_425723 399 12 - - HYPH 10_1101-2021_01_07_425723 399 13 BY by IN 10_1101-2021_01_07_425723 399 14 - - HYPH 10_1101-2021_01_07_425723 399 15 NC NC NNP 10_1101-2021_01_07_425723 399 16 - - HYPH 10_1101-2021_01_07_425723 399 17 ND ND NNP 10_1101-2021_01_07_425723 399 18 4.0 4.0 CD 10_1101-2021_01_07_425723 399 19 International International NNP 10_1101-2021_01_07_425723 399 20 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 399 21 under under IN 10_1101-2021_01_07_425723 399 22 a a DT 10_1101-2021_01_07_425723 399 23 ( ( -LRB- 10_1101-2021_01_07_425723 399 24 which which WDT 10_1101-2021_01_07_425723 399 25 was be VBD 10_1101-2021_01_07_425723 399 26 not not RB 10_1101-2021_01_07_425723 399 27 certified certify VBN 10_1101-2021_01_07_425723 399 28 by by IN 10_1101-2021_01_07_425723 399 29 peer peer NN 10_1101-2021_01_07_425723 399 30 review review NN 10_1101-2021_01_07_425723 399 31 ) ) -RRB- 10_1101-2021_01_07_425723 399 32 is be VBZ 10_1101-2021_01_07_425723 399 33 the the DT 10_1101-2021_01_07_425723 399 34 author author NN 10_1101-2021_01_07_425723 399 35 / / SYM 10_1101-2021_01_07_425723 399 36 funder funder NN 10_1101-2021_01_07_425723 399 37 , , , 10_1101-2021_01_07_425723 399 38 who who WP 10_1101-2021_01_07_425723 399 39 has have VBZ 10_1101-2021_01_07_425723 399 40 granted grant VBN 10_1101-2021_01_07_425723 399 41 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 399 42 a a DT 10_1101-2021_01_07_425723 399 43 license license NN 10_1101-2021_01_07_425723 399 44 to to TO 10_1101-2021_01_07_425723 399 45 display display VB 10_1101-2021_01_07_425723 399 46 the the DT 10_1101-2021_01_07_425723 399 47 preprint preprint NN 10_1101-2021_01_07_425723 399 48 in in IN 10_1101-2021_01_07_425723 399 49 perpetuity perpetuity NN 10_1101-2021_01_07_425723 399 50 . . . 10_1101-2021_01_07_425723 400 1 It -PRON- PRP 10_1101-2021_01_07_425723 400 2 is be VBZ 10_1101-2021_01_07_425723 400 3 made make VBN 10_1101-2021_01_07_425723 400 4 The the DT 10_1101-2021_01_07_425723 400 5 copyright copyright NN 10_1101-2021_01_07_425723 400 6 holder holder NN 10_1101-2021_01_07_425723 400 7 for for IN 10_1101-2021_01_07_425723 400 8 this this DT 10_1101-2021_01_07_425723 400 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 400 10 version version NN 10_1101-2021_01_07_425723 400 11 posted post VBD 10_1101-2021_01_07_425723 400 12 January January NNP 10_1101-2021_01_07_425723 400 13 8 8 CD 10_1101-2021_01_07_425723 400 14 , , , 10_1101-2021_01_07_425723 400 15 2021 2021 CD 10_1101-2021_01_07_425723 400 16 . . . 10_1101-2021_01_07_425723 400 17 ; ; : 10_1101-2021_01_07_425723 400 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 400 19 : : : 10_1101-2021_01_07_425723 400 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 400 21 preprint preprint NN 10_1101-2021_01_07_425723 400 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 400 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CC 10_1101-2021_01_07_425723 400 24 35 35 CD 10_1101-2021_01_07_425723 400 25 into into IN 10_1101-2021_01_07_425723 400 26 Fig Fig NNP 10_1101-2021_01_07_425723 400 27 . . . 10_1101-2021_01_07_425723 401 1 2 2 CD 10_1101-2021_01_07_425723 401 2 , , , 10_1101-2021_01_07_425723 401 3 highlighting highlight VBG 10_1101-2021_01_07_425723 401 4 differentially differentially RB 10_1101-2021_01_07_425723 401 5 expressed express VBN 10_1101-2021_01_07_425723 401 6 genes gene NNS 10_1101-2021_01_07_425723 401 7 found find VBN 10_1101-2021_01_07_425723 401 8 in in IN 10_1101-2021_01_07_425723 401 9 a a DT 10_1101-2021_01_07_425723 401 10 previous previous JJ 10_1101-2021_01_07_425723 401 11 study51 study51 NNS 10_1101-2021_01_07_425723 401 12 . . . 10_1101-2021_01_07_425723 402 1 DGE DGE NNP 10_1101-2021_01_07_425723 402 2 and and CC 10_1101-2021_01_07_425723 402 3 GSA GSA NNP 10_1101-2021_01_07_425723 402 4 were be VBD 10_1101-2021_01_07_425723 402 5 608 608 CD 10_1101-2021_01_07_425723 402 6 performed perform VBN 10_1101-2021_01_07_425723 402 7 using use VBG 10_1101-2021_01_07_425723 402 8 R r NN 10_1101-2021_01_07_425723 402 9 ( ( -LRB- 10_1101-2021_01_07_425723 402 10 v4.0.2)82 v4.0.2)82 NN 10_1101-2021_01_07_425723 402 11 . . . 10_1101-2021_01_07_425723 403 1 609 609 CD 10_1101-2021_01_07_425723 403 2 Anaerobic anaerobic JJ 10_1101-2021_01_07_425723 403 3 growth growth NN 10_1101-2021_01_07_425723 403 4 experiments experiment NNS 10_1101-2021_01_07_425723 403 5 610 610 CD 10_1101-2021_01_07_425723 403 6 Anaerobic anaerobic JJ 10_1101-2021_01_07_425723 403 7 shake shake NN 10_1101-2021_01_07_425723 403 8 - - HYPH 10_1101-2021_01_07_425723 403 9 flask flask NN 10_1101-2021_01_07_425723 403 10 experiments experiment NNS 10_1101-2021_01_07_425723 403 11 were be VBD 10_1101-2021_01_07_425723 403 12 performed perform VBN 10_1101-2021_01_07_425723 403 13 in in IN 10_1101-2021_01_07_425723 403 14 a a DT 10_1101-2021_01_07_425723 403 15 Bactron Bactron NNP 10_1101-2021_01_07_425723 403 16 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 403 17 workstation workstation NN 10_1101-2021_01_07_425723 403 18 ( ( -LRB- 10_1101-2021_01_07_425723 403 19 BACTRON300 BACTRON300 NNP 10_1101-2021_01_07_425723 403 20 - - HYPH 10_1101-2021_01_07_425723 403 21 611 611 CD 10_1101-2021_01_07_425723 403 22 2 2 CD 10_1101-2021_01_07_425723 403 23 , , , 10_1101-2021_01_07_425723 403 24 Sheldon Sheldon NNP 10_1101-2021_01_07_425723 403 25 Manufacturing Manufacturing NNP 10_1101-2021_01_07_425723 403 26 , , , 10_1101-2021_01_07_425723 403 27 Cornelius Cornelius NNP 10_1101-2021_01_07_425723 403 28 , , , 10_1101-2021_01_07_425723 403 29 OR or CC 10_1101-2021_01_07_425723 403 30 ) ) -RRB- 10_1101-2021_01_07_425723 403 31 at at IN 10_1101-2021_01_07_425723 403 32 30 30 CD 10_1101-2021_01_07_425723 403 33 ° ° , 10_1101-2021_01_07_425723 403 34 C C NNP 10_1101-2021_01_07_425723 403 35 . . . 10_1101-2021_01_07_425723 404 1 The the DT 10_1101-2021_01_07_425723 404 2 gas gas NN 10_1101-2021_01_07_425723 404 3 atmosphere atmosphere NN 10_1101-2021_01_07_425723 404 4 consisted consist VBD 10_1101-2021_01_07_425723 404 5 of of IN 10_1101-2021_01_07_425723 404 6 85 85 CD 10_1101-2021_01_07_425723 404 7 % % NN 10_1101-2021_01_07_425723 404 8 N2 N2 NNP 10_1101-2021_01_07_425723 404 9 , , , 10_1101-2021_01_07_425723 404 10 10 10 CD 10_1101-2021_01_07_425723 404 11 % % NN 10_1101-2021_01_07_425723 404 12 CO2 CO2 NNP 10_1101-2021_01_07_425723 404 13 612 612 CD 10_1101-2021_01_07_425723 404 14 and and CC 10_1101-2021_01_07_425723 404 15 5 5 CD 10_1101-2021_01_07_425723 404 16 % % NN 10_1101-2021_01_07_425723 404 17 H2 h2 NN 10_1101-2021_01_07_425723 404 18 and and CC 10_1101-2021_01_07_425723 404 19 was be VBD 10_1101-2021_01_07_425723 404 20 maintained maintain VBN 10_1101-2021_01_07_425723 404 21 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 404 22 by by IN 10_1101-2021_01_07_425723 404 23 a a DT 10_1101-2021_01_07_425723 404 24 Pd Pd NNP 10_1101-2021_01_07_425723 404 25 catalyst catalyst NN 10_1101-2021_01_07_425723 404 26 . . . 10_1101-2021_01_07_425723 405 1 The the DT 10_1101-2021_01_07_425723 405 2 catalyst catalyst NN 10_1101-2021_01_07_425723 405 3 was be VBD 10_1101-2021_01_07_425723 405 4 re re VBN 10_1101-2021_01_07_425723 405 5 - - VBN 10_1101-2021_01_07_425723 405 6 generated generate VBN 10_1101-2021_01_07_425723 405 7 by by IN 10_1101-2021_01_07_425723 405 8 heating heating NN 10_1101-2021_01_07_425723 405 9 till till IN 10_1101-2021_01_07_425723 405 10 613 613 CD 10_1101-2021_01_07_425723 405 11 160 160 CD 10_1101-2021_01_07_425723 405 12 ° ° , 10_1101-2021_01_07_425723 405 13 C c NN 10_1101-2021_01_07_425723 405 14 every every DT 10_1101-2021_01_07_425723 405 15 week week NN 10_1101-2021_01_07_425723 405 16 and and CC 10_1101-2021_01_07_425723 405 17 interchanged interchange VBN 10_1101-2021_01_07_425723 405 18 by by IN 10_1101-2021_01_07_425723 405 19 placing place VBG 10_1101-2021_01_07_425723 405 20 it -PRON- PRP 10_1101-2021_01_07_425723 405 21 in in IN 10_1101-2021_01_07_425723 405 22 the the DT 10_1101-2021_01_07_425723 405 23 airlock airlock NN 10_1101-2021_01_07_425723 405 24 whenever whenever WRB 10_1101-2021_01_07_425723 405 25 the the DT 10_1101-2021_01_07_425723 405 26 pass pass NN 10_1101-2021_01_07_425723 405 27 - - HYPH 10_1101-2021_01_07_425723 405 28 box box NN 10_1101-2021_01_07_425723 405 29 was be VBD 10_1101-2021_01_07_425723 405 30 used use VBN 10_1101-2021_01_07_425723 405 31 . . . 10_1101-2021_01_07_425723 406 1 50-mL 50-ml RB 10_1101-2021_01_07_425723 406 2 614 614 CD 10_1101-2021_01_07_425723 406 3 Shake shake NN 10_1101-2021_01_07_425723 406 4 flasks flask NNS 10_1101-2021_01_07_425723 406 5 were be VBD 10_1101-2021_01_07_425723 406 6 filled fill VBN 10_1101-2021_01_07_425723 406 7 with with IN 10_1101-2021_01_07_425723 406 8 40 40 CD 10_1101-2021_01_07_425723 406 9 mL ml NN 10_1101-2021_01_07_425723 406 10 ( ( -LRB- 10_1101-2021_01_07_425723 406 11 80 80 CD 10_1101-2021_01_07_425723 406 12 % % NN 10_1101-2021_01_07_425723 406 13 volumetric volumetric NN 10_1101-2021_01_07_425723 406 14 ) ) -RRB- 10_1101-2021_01_07_425723 406 15 media medium NNS 10_1101-2021_01_07_425723 406 16 and and CC 10_1101-2021_01_07_425723 406 17 placed place VBN 10_1101-2021_01_07_425723 406 18 on on IN 10_1101-2021_01_07_425723 406 19 an an DT 10_1101-2021_01_07_425723 406 20 orbital orbital JJ 10_1101-2021_01_07_425723 406 21 shaker shaker NN 10_1101-2021_01_07_425723 406 22 ( ( -LRB- 10_1101-2021_01_07_425723 406 23 KS KS NNP 10_1101-2021_01_07_425723 406 24 130 130 CD 10_1101-2021_01_07_425723 406 25 615 615 CD 10_1101-2021_01_07_425723 406 26 basic basic JJ 10_1101-2021_01_07_425723 406 27 , , , 10_1101-2021_01_07_425723 406 28 IKA IKA NNP 10_1101-2021_01_07_425723 406 29 , , , 10_1101-2021_01_07_425723 406 30 Staufen Staufen NNP 10_1101-2021_01_07_425723 406 31 , , , 10_1101-2021_01_07_425723 406 32 Germany Germany NNP 10_1101-2021_01_07_425723 406 33 ) ) -RRB- 10_1101-2021_01_07_425723 406 34 set set VBD 10_1101-2021_01_07_425723 406 35 at at IN 10_1101-2021_01_07_425723 406 36 240 240 CD 10_1101-2021_01_07_425723 406 37 rpm rpm NN 10_1101-2021_01_07_425723 406 38 inside inside IN 10_1101-2021_01_07_425723 406 39 the the DT 10_1101-2021_01_07_425723 406 40 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 406 41 chamber chamber NN 10_1101-2021_01_07_425723 406 42 . . . 10_1101-2021_01_07_425723 407 1 Sterile sterile JJ 10_1101-2021_01_07_425723 407 2 growth growth NN 10_1101-2021_01_07_425723 407 3 media medium NNS 10_1101-2021_01_07_425723 407 4 was be VBD 10_1101-2021_01_07_425723 407 5 616 616 CD 10_1101-2021_01_07_425723 407 6 placed place VBN 10_1101-2021_01_07_425723 407 7 inside inside IN 10_1101-2021_01_07_425723 407 8 the the DT 10_1101-2021_01_07_425723 407 9 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 407 10 chamber chamber NNP 10_1101-2021_01_07_425723 407 11 24 24 CD 10_1101-2021_01_07_425723 407 12 h h NNS 10_1101-2021_01_07_425723 407 13 prior prior RB 10_1101-2021_01_07_425723 407 14 to to IN 10_1101-2021_01_07_425723 407 15 inoculation inoculation NN 10_1101-2021_01_07_425723 407 16 to to TO 10_1101-2021_01_07_425723 407 17 ensure ensure VB 10_1101-2021_01_07_425723 407 18 complete complete JJ 10_1101-2021_01_07_425723 407 19 removal removal NN 10_1101-2021_01_07_425723 407 20 of of IN 10_1101-2021_01_07_425723 407 21 traces trace NNS 10_1101-2021_01_07_425723 407 22 of of IN 10_1101-2021_01_07_425723 407 23 617 617 CD 10_1101-2021_01_07_425723 407 24 oxygen oxygen NN 10_1101-2021_01_07_425723 407 25 . . . 10_1101-2021_01_07_425723 408 1 618 618 CD 10_1101-2021_01_07_425723 408 2 The the DT 10_1101-2021_01_07_425723 408 3 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 408 4 growth growth NN 10_1101-2021_01_07_425723 408 5 ability ability NN 10_1101-2021_01_07_425723 408 6 of of IN 10_1101-2021_01_07_425723 408 7 the the DT 10_1101-2021_01_07_425723 408 8 yeast yeast NN 10_1101-2021_01_07_425723 408 9 strains strain NNS 10_1101-2021_01_07_425723 408 10 was be VBD 10_1101-2021_01_07_425723 408 11 tested test VBN 10_1101-2021_01_07_425723 408 12 on on IN 10_1101-2021_01_07_425723 408 13 SMG SMG NNP 10_1101-2021_01_07_425723 408 14 - - HYPH 10_1101-2021_01_07_425723 408 15 urea urea NNP 10_1101-2021_01_07_425723 408 16 with with IN 10_1101-2021_01_07_425723 408 17 50 50 CD 10_1101-2021_01_07_425723 408 18 g·L-1 g·l-1 CD 10_1101-2021_01_07_425723 408 19 glucose glucose NN 10_1101-2021_01_07_425723 408 20 at at IN 10_1101-2021_01_07_425723 408 21 pH pH NNP 10_1101-2021_01_07_425723 408 22 6.0 6.0 CD 10_1101-2021_01_07_425723 408 23 619 619 CD 10_1101-2021_01_07_425723 408 24 with with IN 10_1101-2021_01_07_425723 408 25 Tween Tween NNP 10_1101-2021_01_07_425723 408 26 80 80 CD 10_1101-2021_01_07_425723 408 27 prepared prepare VBD 10_1101-2021_01_07_425723 408 28 as as IN 10_1101-2021_01_07_425723 408 29 described describe VBN 10_1101-2021_01_07_425723 408 30 earlier early RBR 10_1101-2021_01_07_425723 408 31 . . . 10_1101-2021_01_07_425723 409 1 The the DT 10_1101-2021_01_07_425723 409 2 growth growth NN 10_1101-2021_01_07_425723 409 3 experiments experiment NNS 10_1101-2021_01_07_425723 409 4 were be VBD 10_1101-2021_01_07_425723 409 5 started start VBN 10_1101-2021_01_07_425723 409 6 from from IN 10_1101-2021_01_07_425723 409 7 aerobic aerobic JJ 10_1101-2021_01_07_425723 409 8 pre-620 pre-620 NN 10_1101-2021_01_07_425723 409 9 cultures culture NNS 10_1101-2021_01_07_425723 409 10 on on IN 10_1101-2021_01_07_425723 409 11 SMG SMG NNP 10_1101-2021_01_07_425723 409 12 - - HYPH 10_1101-2021_01_07_425723 409 13 urea urea NNP 10_1101-2021_01_07_425723 409 14 media medium NNS 10_1101-2021_01_07_425723 409 15 and and CC 10_1101-2021_01_07_425723 409 16 the the DT 10_1101-2021_01_07_425723 409 17 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 409 18 shake shake NN 10_1101-2021_01_07_425723 409 19 flasks flask NNS 10_1101-2021_01_07_425723 409 20 were be VBD 10_1101-2021_01_07_425723 409 21 inoculated inoculate VBN 10_1101-2021_01_07_425723 409 22 at at IN 10_1101-2021_01_07_425723 409 23 an an DT 10_1101-2021_01_07_425723 409 24 OD660 od660 NN 10_1101-2021_01_07_425723 409 25 of of IN 10_1101-2021_01_07_425723 409 26 0.2 0.2 CD 10_1101-2021_01_07_425723 409 27 621 621 CD 10_1101-2021_01_07_425723 409 28 ( ( -LRB- 10_1101-2021_01_07_425723 409 29 corresponding correspond VBG 10_1101-2021_01_07_425723 409 30 to to IN 10_1101-2021_01_07_425723 409 31 an an DT 10_1101-2021_01_07_425723 409 32 OD600 od600 NN 10_1101-2021_01_07_425723 409 33 of of IN 10_1101-2021_01_07_425723 409 34 0.14 0.14 CD 10_1101-2021_01_07_425723 409 35 ) ) -RRB- 10_1101-2021_01_07_425723 409 36 . . . 10_1101-2021_01_07_425723 410 1 In in IN 10_1101-2021_01_07_425723 410 2 order order NN 10_1101-2021_01_07_425723 410 3 to to TO 10_1101-2021_01_07_425723 410 4 minimize minimize VB 10_1101-2021_01_07_425723 410 5 opening open VBG 10_1101-2021_01_07_425723 410 6 the the DT 10_1101-2021_01_07_425723 410 7 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 410 8 chamber chamber NN 10_1101-2021_01_07_425723 410 9 , , , 10_1101-2021_01_07_425723 410 10 culture culture NN 10_1101-2021_01_07_425723 410 11 622 622 CD 10_1101-2021_01_07_425723 410 12 growth growth NN 10_1101-2021_01_07_425723 410 13 was be VBD 10_1101-2021_01_07_425723 410 14 monitored monitor VBN 10_1101-2021_01_07_425723 410 15 by by IN 10_1101-2021_01_07_425723 410 16 optical optical JJ 10_1101-2021_01_07_425723 410 17 density density NN 10_1101-2021_01_07_425723 410 18 measurements measurement NNS 10_1101-2021_01_07_425723 410 19 inside inside IN 10_1101-2021_01_07_425723 410 20 the the DT 10_1101-2021_01_07_425723 410 21 chamber chamber NN 10_1101-2021_01_07_425723 410 22 using use VBG 10_1101-2021_01_07_425723 410 23 an an DT 10_1101-2021_01_07_425723 410 24 Ultrospec Ultrospec NNP 10_1101-2021_01_07_425723 410 25 10 10 CD 10_1101-2021_01_07_425723 410 26 cell cell NN 10_1101-2021_01_07_425723 410 27 623 623 CD 10_1101-2021_01_07_425723 410 28 density density NN 10_1101-2021_01_07_425723 410 29 meter meter NN 10_1101-2021_01_07_425723 410 30 ( ( -LRB- 10_1101-2021_01_07_425723 410 31 Biochrom Biochrom NNP 10_1101-2021_01_07_425723 410 32 , , , 10_1101-2021_01_07_425723 410 33 Cambridge Cambridge NNP 10_1101-2021_01_07_425723 410 34 , , , 10_1101-2021_01_07_425723 410 35 UK UK NNP 10_1101-2021_01_07_425723 410 36 ) ) -RRB- 10_1101-2021_01_07_425723 410 37 at at IN 10_1101-2021_01_07_425723 410 38 a a DT 10_1101-2021_01_07_425723 410 39 600 600 CD 10_1101-2021_01_07_425723 410 40 nm nm CD 10_1101-2021_01_07_425723 410 41 wavelength wavelength NN 10_1101-2021_01_07_425723 410 42 . . . 10_1101-2021_01_07_425723 411 1 When when WRB 10_1101-2021_01_07_425723 411 2 the the DT 10_1101-2021_01_07_425723 411 3 optical optical JJ 10_1101-2021_01_07_425723 411 4 density density NN 10_1101-2021_01_07_425723 411 5 of of IN 10_1101-2021_01_07_425723 411 6 culture culture NN 10_1101-2021_01_07_425723 411 7 624 624 CD 10_1101-2021_01_07_425723 411 8 no no RB 10_1101-2021_01_07_425723 411 9 longer long RBR 10_1101-2021_01_07_425723 411 10 increased increase VBD 10_1101-2021_01_07_425723 411 11 or or CC 10_1101-2021_01_07_425723 411 12 decreased decrease VBD 10_1101-2021_01_07_425723 411 13 new new JJ 10_1101-2021_01_07_425723 411 14 shake shake NN 10_1101-2021_01_07_425723 411 15 - - HYPH 10_1101-2021_01_07_425723 411 16 flask flask NN 10_1101-2021_01_07_425723 411 17 cultures culture NNS 10_1101-2021_01_07_425723 411 18 were be VBD 10_1101-2021_01_07_425723 411 19 inoculated inoculate VBN 10_1101-2021_01_07_425723 411 20 by by IN 10_1101-2021_01_07_425723 411 21 serial serial JJ 10_1101-2021_01_07_425723 411 22 transfer transfer NN 10_1101-2021_01_07_425723 411 23 at at IN 10_1101-2021_01_07_425723 411 24 an an DT 10_1101-2021_01_07_425723 411 25 initial initial JJ 10_1101-2021_01_07_425723 411 26 625 625 CD 10_1101-2021_01_07_425723 411 27 OD600 od600 NN 10_1101-2021_01_07_425723 411 28 of of IN 10_1101-2021_01_07_425723 411 29 0.2 0.2 CD 10_1101-2021_01_07_425723 411 30 . . . 10_1101-2021_01_07_425723 412 1 626 626 CD 10_1101-2021_01_07_425723 412 2 Laboratory laboratory NN 10_1101-2021_01_07_425723 412 3 evolution evolution NN 10_1101-2021_01_07_425723 412 4 in in IN 10_1101-2021_01_07_425723 412 5 low low JJ 10_1101-2021_01_07_425723 412 6 oxygen oxygen NN 10_1101-2021_01_07_425723 412 7 atmosphere atmosphere NN 10_1101-2021_01_07_425723 412 8 627 627 CD 10_1101-2021_01_07_425723 412 9 Adaptive adaptive JJ 10_1101-2021_01_07_425723 412 10 laboratory laboratory NN 10_1101-2021_01_07_425723 412 11 evolution evolution NN 10_1101-2021_01_07_425723 412 12 for for IN 10_1101-2021_01_07_425723 412 13 strict strict JJ 10_1101-2021_01_07_425723 412 14 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 412 15 growth growth NN 10_1101-2021_01_07_425723 412 16 was be VBD 10_1101-2021_01_07_425723 412 17 performed perform VBN 10_1101-2021_01_07_425723 412 18 in in IN 10_1101-2021_01_07_425723 412 19 a a DT 10_1101-2021_01_07_425723 412 20 Bactron Bactron NNP 10_1101-2021_01_07_425723 412 21 anaerobic anaerobic NN 10_1101-2021_01_07_425723 412 22 628 628 CD 10_1101-2021_01_07_425723 412 23 workstation workstation NN 10_1101-2021_01_07_425723 412 24 ( ( -LRB- 10_1101-2021_01_07_425723 412 25 BACTRON BACTRON NNP 10_1101-2021_01_07_425723 412 26 BAC BAC NNP 10_1101-2021_01_07_425723 412 27 - - HYPH 10_1101-2021_01_07_425723 412 28 X-2E X-2E NNP 10_1101-2021_01_07_425723 412 29 , , , 10_1101-2021_01_07_425723 412 30 Sheldon Sheldon NNP 10_1101-2021_01_07_425723 412 31 Manufacturing Manufacturing NNP 10_1101-2021_01_07_425723 412 32 ) ) -RRB- 10_1101-2021_01_07_425723 412 33 at at IN 10_1101-2021_01_07_425723 412 34 30 30 CD 10_1101-2021_01_07_425723 412 35 ° ° , 10_1101-2021_01_07_425723 412 36 C C NNP 10_1101-2021_01_07_425723 412 37 . . . 10_1101-2021_01_07_425723 413 1 50-mL 50-ml JJ 10_1101-2021_01_07_425723 413 2 Shake shake NN 10_1101-2021_01_07_425723 413 3 flasks flask NNS 10_1101-2021_01_07_425723 413 4 were be VBD 10_1101-2021_01_07_425723 413 5 filled fill VBN 10_1101-2021_01_07_425723 413 6 with with IN 10_1101-2021_01_07_425723 413 7 629 629 CD 10_1101-2021_01_07_425723 413 8 .CC .CC , 10_1101-2021_01_07_425723 413 9 - - HYPH 10_1101-2021_01_07_425723 413 10 BY by IN 10_1101-2021_01_07_425723 413 11 - - HYPH 10_1101-2021_01_07_425723 413 12 NC NC NNP 10_1101-2021_01_07_425723 413 13 - - HYPH 10_1101-2021_01_07_425723 413 14 ND ND NNP 10_1101-2021_01_07_425723 413 15 4.0 4.0 CD 10_1101-2021_01_07_425723 413 16 International International NNP 10_1101-2021_01_07_425723 413 17 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 413 18 under under IN 10_1101-2021_01_07_425723 413 19 a a DT 10_1101-2021_01_07_425723 413 20 ( ( -LRB- 10_1101-2021_01_07_425723 413 21 which which WDT 10_1101-2021_01_07_425723 413 22 was be VBD 10_1101-2021_01_07_425723 413 23 not not RB 10_1101-2021_01_07_425723 413 24 certified certify VBN 10_1101-2021_01_07_425723 413 25 by by IN 10_1101-2021_01_07_425723 413 26 peer peer NN 10_1101-2021_01_07_425723 413 27 review review NN 10_1101-2021_01_07_425723 413 28 ) ) -RRB- 10_1101-2021_01_07_425723 413 29 is be VBZ 10_1101-2021_01_07_425723 413 30 the the DT 10_1101-2021_01_07_425723 413 31 author author NN 10_1101-2021_01_07_425723 413 32 / / SYM 10_1101-2021_01_07_425723 413 33 funder funder NN 10_1101-2021_01_07_425723 413 34 , , , 10_1101-2021_01_07_425723 413 35 who who WP 10_1101-2021_01_07_425723 413 36 has have VBZ 10_1101-2021_01_07_425723 413 37 granted grant VBN 10_1101-2021_01_07_425723 413 38 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 413 39 a a DT 10_1101-2021_01_07_425723 413 40 license license NN 10_1101-2021_01_07_425723 413 41 to to TO 10_1101-2021_01_07_425723 413 42 display display VB 10_1101-2021_01_07_425723 413 43 the the DT 10_1101-2021_01_07_425723 413 44 preprint preprint NN 10_1101-2021_01_07_425723 413 45 in in IN 10_1101-2021_01_07_425723 413 46 perpetuity perpetuity NN 10_1101-2021_01_07_425723 413 47 . . . 10_1101-2021_01_07_425723 414 1 It -PRON- PRP 10_1101-2021_01_07_425723 414 2 is be VBZ 10_1101-2021_01_07_425723 414 3 made make VBN 10_1101-2021_01_07_425723 414 4 The the DT 10_1101-2021_01_07_425723 414 5 copyright copyright NN 10_1101-2021_01_07_425723 414 6 holder holder NN 10_1101-2021_01_07_425723 414 7 for for IN 10_1101-2021_01_07_425723 414 8 this this DT 10_1101-2021_01_07_425723 414 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 414 10 version version NN 10_1101-2021_01_07_425723 414 11 posted post VBD 10_1101-2021_01_07_425723 414 12 January January NNP 10_1101-2021_01_07_425723 414 13 8 8 CD 10_1101-2021_01_07_425723 414 14 , , , 10_1101-2021_01_07_425723 414 15 2021 2021 CD 10_1101-2021_01_07_425723 414 16 . . . 10_1101-2021_01_07_425723 414 17 ; ; : 10_1101-2021_01_07_425723 414 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 414 19 : : : 10_1101-2021_01_07_425723 414 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 414 21 preprint preprint NN 10_1101-2021_01_07_425723 414 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 414 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 414 24 36 36 CD 10_1101-2021_01_07_425723 414 25 40 40 CD 10_1101-2021_01_07_425723 414 26 mL mL NNP 10_1101-2021_01_07_425723 414 27 SMG SMG NNP 10_1101-2021_01_07_425723 414 28 - - HYPH 10_1101-2021_01_07_425723 414 29 urea urea NNP 10_1101-2021_01_07_425723 414 30 with with IN 10_1101-2021_01_07_425723 414 31 50 50 CD 10_1101-2021_01_07_425723 414 32 g·L-1 g·l-1 CD 10_1101-2021_01_07_425723 414 33 glucose glucose NN 10_1101-2021_01_07_425723 414 34 and and CC 10_1101-2021_01_07_425723 414 35 including include VBG 10_1101-2021_01_07_425723 414 36 420 420 CD 10_1101-2021_01_07_425723 414 37 mg·L-1 mg·l-1 NN 10_1101-2021_01_07_425723 414 38 Tween Tween NNP 10_1101-2021_01_07_425723 414 39 80 80 CD 10_1101-2021_01_07_425723 414 40 . . . 10_1101-2021_01_07_425723 415 1 Subsequently subsequently RB 10_1101-2021_01_07_425723 415 2 the the DT 10_1101-2021_01_07_425723 415 3 shake shake NN 10_1101-2021_01_07_425723 415 4 - - HYPH 10_1101-2021_01_07_425723 415 5 flask flask NN 10_1101-2021_01_07_425723 415 6 630 630 CD 10_1101-2021_01_07_425723 415 7 media medium NNS 10_1101-2021_01_07_425723 415 8 were be VBD 10_1101-2021_01_07_425723 415 9 inoculated inoculate VBN 10_1101-2021_01_07_425723 415 10 with with IN 10_1101-2021_01_07_425723 415 11 IMX2323 IMX2323 NNP 10_1101-2021_01_07_425723 415 12 from from IN 10_1101-2021_01_07_425723 415 13 glycerol glycerol NNP 10_1101-2021_01_07_425723 415 14 cryo cryo NN 10_1101-2021_01_07_425723 415 15 - - HYPH 10_1101-2021_01_07_425723 415 16 stock stock NN 10_1101-2021_01_07_425723 415 17 at at IN 10_1101-2021_01_07_425723 415 18 OD660 od660 NN 10_1101-2021_01_07_425723 415 19 < < XX 10_1101-2021_01_07_425723 415 20 0.01 0.01 XX 10_1101-2021_01_07_425723 415 21 and and CC 10_1101-2021_01_07_425723 415 22 thereafter thereafter RB 10_1101-2021_01_07_425723 415 23 placed place VBD 10_1101-2021_01_07_425723 415 24 631 631 CD 10_1101-2021_01_07_425723 415 25 inside inside IN 10_1101-2021_01_07_425723 415 26 the the DT 10_1101-2021_01_07_425723 415 27 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 415 28 chamber chamber NN 10_1101-2021_01_07_425723 415 29 . . . 10_1101-2021_01_07_425723 416 1 Due due IN 10_1101-2021_01_07_425723 416 2 to to IN 10_1101-2021_01_07_425723 416 3 frequent frequent JJ 10_1101-2021_01_07_425723 416 4 opening opening NN 10_1101-2021_01_07_425723 416 5 of of IN 10_1101-2021_01_07_425723 416 6 the the DT 10_1101-2021_01_07_425723 416 7 pass pass NN 10_1101-2021_01_07_425723 416 8 - - HYPH 10_1101-2021_01_07_425723 416 9 box box NN 10_1101-2021_01_07_425723 416 10 and and CC 10_1101-2021_01_07_425723 416 11 lack lack NN 10_1101-2021_01_07_425723 416 12 of of IN 10_1101-2021_01_07_425723 416 13 catalyst catalyst NN 10_1101-2021_01_07_425723 416 14 inside inside IN 10_1101-2021_01_07_425723 416 15 the the DT 10_1101-2021_01_07_425723 416 16 632 632 CD 10_1101-2021_01_07_425723 416 17 pass pass NN 10_1101-2021_01_07_425723 416 18 - - HYPH 10_1101-2021_01_07_425723 416 19 box box NN 10_1101-2021_01_07_425723 416 20 oxygen oxygen NN 10_1101-2021_01_07_425723 416 21 entry entry NN 10_1101-2021_01_07_425723 416 22 was be VBD 10_1101-2021_01_07_425723 416 23 more more RBR 10_1101-2021_01_07_425723 416 24 permissive permissive JJ 10_1101-2021_01_07_425723 416 25 . . . 10_1101-2021_01_07_425723 417 1 After after IN 10_1101-2021_01_07_425723 417 2 the the DT 10_1101-2021_01_07_425723 417 3 optical optical JJ 10_1101-2021_01_07_425723 417 4 density density NN 10_1101-2021_01_07_425723 417 5 of of IN 10_1101-2021_01_07_425723 417 6 the the DT 10_1101-2021_01_07_425723 417 7 cultures culture NNS 10_1101-2021_01_07_425723 417 8 no no RB 10_1101-2021_01_07_425723 417 9 longer long RBR 10_1101-2021_01_07_425723 417 10 633 633 CD 10_1101-2021_01_07_425723 417 11 increased increase VBD 10_1101-2021_01_07_425723 417 12 , , , 10_1101-2021_01_07_425723 417 13 cultures culture NNS 10_1101-2021_01_07_425723 417 14 were be VBD 10_1101-2021_01_07_425723 417 15 transferred transfer VBN 10_1101-2021_01_07_425723 417 16 to to IN 10_1101-2021_01_07_425723 417 17 new new JJ 10_1101-2021_01_07_425723 417 18 media medium NNS 10_1101-2021_01_07_425723 417 19 by by IN 10_1101-2021_01_07_425723 417 20 40 40 CD 10_1101-2021_01_07_425723 417 21 - - HYPH 10_1101-2021_01_07_425723 417 22 50x 50x NNS 10_1101-2021_01_07_425723 417 23 serial serial JJ 10_1101-2021_01_07_425723 417 24 dilution dilution NN 10_1101-2021_01_07_425723 417 25 . . . 10_1101-2021_01_07_425723 418 1 For for IN 10_1101-2021_01_07_425723 418 2 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 418 3 , , , 10_1101-2021_01_07_425723 418 4 IMS1112 IMS1112 NNP 10_1101-2021_01_07_425723 418 5 , , , 10_1101-2021_01_07_425723 418 6 634 634 CD 10_1101-2021_01_07_425723 418 7 IMS1113 ims1113 NN 10_1101-2021_01_07_425723 418 8 three three CD 10_1101-2021_01_07_425723 418 9 and and CC 10_1101-2021_01_07_425723 418 10 for for IN 10_1101-2021_01_07_425723 418 11 IMS1131 IMS1131 NNP 10_1101-2021_01_07_425723 418 12 , , , 10_1101-2021_01_07_425723 418 13 IMS1132 IMS1132 NNP 10_1101-2021_01_07_425723 418 14 , , , 10_1101-2021_01_07_425723 418 15 IMS1133 IMS1133 NNP 10_1101-2021_01_07_425723 418 16 four four CD 10_1101-2021_01_07_425723 418 17 serial serial JJ 10_1101-2021_01_07_425723 418 18 transfers transfer NNS 10_1101-2021_01_07_425723 418 19 in in IN 10_1101-2021_01_07_425723 418 20 shake shake NN 10_1101-2021_01_07_425723 418 21 - - HYPH 10_1101-2021_01_07_425723 418 22 flask flask NN 10_1101-2021_01_07_425723 418 23 media medium NNS 10_1101-2021_01_07_425723 418 24 were be VBD 10_1101-2021_01_07_425723 418 25 635 635 CD 10_1101-2021_01_07_425723 418 26 performed perform VBN 10_1101-2021_01_07_425723 418 27 after after IN 10_1101-2021_01_07_425723 418 28 which which WDT 10_1101-2021_01_07_425723 418 29 single single JJ 10_1101-2021_01_07_425723 418 30 colony colony NN 10_1101-2021_01_07_425723 418 31 isolates isolate NNS 10_1101-2021_01_07_425723 418 32 were be VBD 10_1101-2021_01_07_425723 418 33 made make VBN 10_1101-2021_01_07_425723 418 34 by by IN 10_1101-2021_01_07_425723 418 35 plating plate VBG 10_1101-2021_01_07_425723 418 36 on on IN 10_1101-2021_01_07_425723 418 37 YPD YPD NNP 10_1101-2021_01_07_425723 418 38 agar agar NN 10_1101-2021_01_07_425723 418 39 media medium NNS 10_1101-2021_01_07_425723 418 40 with with IN 10_1101-2021_01_07_425723 418 41 hygromycin hygromycin NNP 10_1101-2021_01_07_425723 418 42 636 636 CD 10_1101-2021_01_07_425723 418 43 antibiotic antibiotic JJ 10_1101-2021_01_07_425723 418 44 at at IN 10_1101-2021_01_07_425723 418 45 30 30 CD 10_1101-2021_01_07_425723 418 46 ° ° , 10_1101-2021_01_07_425723 418 47 C C NNP 10_1101-2021_01_07_425723 418 48 aerobically aerobically RB 10_1101-2021_01_07_425723 418 49 . . . 10_1101-2021_01_07_425723 419 1 Single single JJ 10_1101-2021_01_07_425723 419 2 colony colony NN 10_1101-2021_01_07_425723 419 3 isolates isolate NNS 10_1101-2021_01_07_425723 419 4 were be VBD 10_1101-2021_01_07_425723 419 5 subsequently subsequently RB 10_1101-2021_01_07_425723 419 6 restreaked restreake VBN 10_1101-2021_01_07_425723 419 7 sequentially sequentially RB 10_1101-2021_01_07_425723 419 8 for for IN 10_1101-2021_01_07_425723 419 9 637 637 CD 10_1101-2021_01_07_425723 419 10 three three CD 10_1101-2021_01_07_425723 419 11 times time NNS 10_1101-2021_01_07_425723 419 12 on on IN 10_1101-2021_01_07_425723 419 13 the the DT 10_1101-2021_01_07_425723 419 14 same same JJ 10_1101-2021_01_07_425723 419 15 media medium NNS 10_1101-2021_01_07_425723 419 16 before before IN 10_1101-2021_01_07_425723 419 17 the the DT 10_1101-2021_01_07_425723 419 18 isolates isolate NNS 10_1101-2021_01_07_425723 419 19 were be VBD 10_1101-2021_01_07_425723 419 20 propagated propagate VBN 10_1101-2021_01_07_425723 419 21 in in IN 10_1101-2021_01_07_425723 419 22 SM SM NNP 10_1101-2021_01_07_425723 419 23 glucose glucose NNP 10_1101-2021_01_07_425723 419 24 media medium NNS 10_1101-2021_01_07_425723 419 25 and and CC 10_1101-2021_01_07_425723 419 26 glycerol glycerol NN 10_1101-2021_01_07_425723 419 27 638 638 CD 10_1101-2021_01_07_425723 419 28 cryo cryo NN 10_1101-2021_01_07_425723 419 29 stocked stock VBN 10_1101-2021_01_07_425723 419 30 . . . 10_1101-2021_01_07_425723 420 1 639 639 CD 10_1101-2021_01_07_425723 420 2 To to TO 10_1101-2021_01_07_425723 420 3 determine determine VB 10_1101-2021_01_07_425723 420 4 if if IN 10_1101-2021_01_07_425723 420 5 an an DT 10_1101-2021_01_07_425723 420 6 oxygen oxygen NN 10_1101-2021_01_07_425723 420 7 - - HYPH 10_1101-2021_01_07_425723 420 8 limited limit VBN 10_1101-2021_01_07_425723 420 9 pre pre JJ 10_1101-2021_01_07_425723 420 10 - - NN 10_1101-2021_01_07_425723 420 11 culture culture NN 10_1101-2021_01_07_425723 420 12 was be VBD 10_1101-2021_01_07_425723 420 13 required require VBN 10_1101-2021_01_07_425723 420 14 for for IN 10_1101-2021_01_07_425723 420 15 the the DT 10_1101-2021_01_07_425723 420 16 strict strict JJ 10_1101-2021_01_07_425723 420 17 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 420 18 growth growth NN 10_1101-2021_01_07_425723 420 19 of of IN 10_1101-2021_01_07_425723 420 20 IMX2323 IMX2323 NNP 10_1101-2021_01_07_425723 420 21 640 640 CD 10_1101-2021_01_07_425723 420 22 strain strain VBP 10_1101-2021_01_07_425723 420 23 a a DT 10_1101-2021_01_07_425723 420 24 cross cross JJ 10_1101-2021_01_07_425723 420 25 - - JJ 10_1101-2021_01_07_425723 420 26 validation validation JJ 10_1101-2021_01_07_425723 420 27 experiment experiment NN 10_1101-2021_01_07_425723 420 28 was be VBD 10_1101-2021_01_07_425723 420 29 performed perform VBN 10_1101-2021_01_07_425723 420 30 . . . 10_1101-2021_01_07_425723 421 1 In in IN 10_1101-2021_01_07_425723 421 2 parallel parallel NN 10_1101-2021_01_07_425723 421 3 , , , 10_1101-2021_01_07_425723 421 4 yeast yeast NN 10_1101-2021_01_07_425723 421 5 strains strain NNS 10_1101-2021_01_07_425723 421 6 were be VBD 10_1101-2021_01_07_425723 421 7 cultivated cultivate VBN 10_1101-2021_01_07_425723 421 8 in in IN 10_1101-2021_01_07_425723 421 9 50-mL 50-ml RB 10_1101-2021_01_07_425723 421 10 641 641 CD 10_1101-2021_01_07_425723 421 11 shake shake NN 10_1101-2021_01_07_425723 421 12 - - HYPH 10_1101-2021_01_07_425723 421 13 flask flask NN 10_1101-2021_01_07_425723 421 14 cultures culture NNS 10_1101-2021_01_07_425723 421 15 with with IN 10_1101-2021_01_07_425723 421 16 SMG SMG NNP 10_1101-2021_01_07_425723 421 17 - - HYPH 10_1101-2021_01_07_425723 421 18 urea urea NNP 10_1101-2021_01_07_425723 421 19 with with IN 10_1101-2021_01_07_425723 421 20 50 50 CD 10_1101-2021_01_07_425723 421 21 g·L-1 g·l-1 CD 10_1101-2021_01_07_425723 421 22 glucose glucose NN 10_1101-2021_01_07_425723 421 23 at at IN 10_1101-2021_01_07_425723 421 24 pH pH NNP 10_1101-2021_01_07_425723 421 25 6.0 6.0 CD 10_1101-2021_01_07_425723 421 26 with with IN 10_1101-2021_01_07_425723 421 27 Tween Tween NNP 10_1101-2021_01_07_425723 421 28 80 80 CD 10_1101-2021_01_07_425723 421 29 in in IN 10_1101-2021_01_07_425723 421 30 both both CC 10_1101-2021_01_07_425723 421 31 the the DT 10_1101-2021_01_07_425723 421 32 Bactron Bactron NNP 10_1101-2021_01_07_425723 421 33 642 642 CD 10_1101-2021_01_07_425723 421 34 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 421 35 workstation workstation NN 10_1101-2021_01_07_425723 421 36 ( ( -LRB- 10_1101-2021_01_07_425723 421 37 BACTRON BACTRON NNP 10_1101-2021_01_07_425723 421 38 BAC BAC NNP 10_1101-2021_01_07_425723 421 39 - - HYPH 10_1101-2021_01_07_425723 421 40 X-2E X-2E NNP 10_1101-2021_01_07_425723 421 41 , , , 10_1101-2021_01_07_425723 421 42 Sheldon Sheldon NNP 10_1101-2021_01_07_425723 421 43 Manufacturing Manufacturing NNP 10_1101-2021_01_07_425723 421 44 ) ) -RRB- 10_1101-2021_01_07_425723 421 45 with with IN 10_1101-2021_01_07_425723 421 46 low low JJ 10_1101-2021_01_07_425723 421 47 levels level NNS 10_1101-2021_01_07_425723 421 48 of of IN 10_1101-2021_01_07_425723 421 49 oxygen-643 oxygen-643 JJ 10_1101-2021_01_07_425723 421 50 contamination contamination NN 10_1101-2021_01_07_425723 421 51 , , , 10_1101-2021_01_07_425723 421 52 and and CC 10_1101-2021_01_07_425723 421 53 in in IN 10_1101-2021_01_07_425723 421 54 the the DT 10_1101-2021_01_07_425723 421 55 Bactron Bactron NNP 10_1101-2021_01_07_425723 421 56 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 421 57 workstation workstation NN 10_1101-2021_01_07_425723 421 58 ( ( -LRB- 10_1101-2021_01_07_425723 421 59 BACTRON300 BACTRON300 NNP 10_1101-2021_01_07_425723 421 60 - - HYPH 10_1101-2021_01_07_425723 421 61 2 2 CD 10_1101-2021_01_07_425723 421 62 , , , 10_1101-2021_01_07_425723 421 63 Sheldon Sheldon NNP 10_1101-2021_01_07_425723 421 64 Manufacturing Manufacturing NNP 10_1101-2021_01_07_425723 421 65 ) ) -RRB- 10_1101-2021_01_07_425723 421 66 with with IN 10_1101-2021_01_07_425723 421 67 644 644 CD 10_1101-2021_01_07_425723 421 68 strict strict JJ 10_1101-2021_01_07_425723 421 69 control control NN 10_1101-2021_01_07_425723 421 70 of of IN 10_1101-2021_01_07_425723 421 71 oxygen oxygen NN 10_1101-2021_01_07_425723 421 72 - - HYPH 10_1101-2021_01_07_425723 421 73 contamination contamination NN 10_1101-2021_01_07_425723 421 74 . . . 10_1101-2021_01_07_425723 422 1 After after IN 10_1101-2021_01_07_425723 422 2 stagnation stagnation NN 10_1101-2021_01_07_425723 422 3 of of IN 10_1101-2021_01_07_425723 422 4 growth growth NN 10_1101-2021_01_07_425723 422 5 was be VBD 10_1101-2021_01_07_425723 422 6 observed observe VBN 10_1101-2021_01_07_425723 422 7 in in IN 10_1101-2021_01_07_425723 422 8 the the DT 10_1101-2021_01_07_425723 422 9 second second JJ 10_1101-2021_01_07_425723 422 10 serial serial JJ 10_1101-2021_01_07_425723 422 11 645 645 CD 10_1101-2021_01_07_425723 422 12 transfer transfer NN 10_1101-2021_01_07_425723 422 13 of of IN 10_1101-2021_01_07_425723 422 14 the the DT 10_1101-2021_01_07_425723 422 15 shake shake NN 10_1101-2021_01_07_425723 422 16 - - HYPH 10_1101-2021_01_07_425723 422 17 flask flask NN 10_1101-2021_01_07_425723 422 18 cultures culture VBZ 10_1101-2021_01_07_425723 422 19 a a DT 10_1101-2021_01_07_425723 422 20 1.5 1.5 CD 10_1101-2021_01_07_425723 422 21 mL ml NN 10_1101-2021_01_07_425723 422 22 sample sample NN 10_1101-2021_01_07_425723 422 23 of of IN 10_1101-2021_01_07_425723 422 24 each each DT 10_1101-2021_01_07_425723 422 25 culture culture NN 10_1101-2021_01_07_425723 422 26 was be VBD 10_1101-2021_01_07_425723 422 27 taken take VBN 10_1101-2021_01_07_425723 422 28 , , , 10_1101-2021_01_07_425723 422 29 sealed seal VBN 10_1101-2021_01_07_425723 422 30 , , , 10_1101-2021_01_07_425723 422 31 and and CC 10_1101-2021_01_07_425723 422 32 used use VBD 10_1101-2021_01_07_425723 422 33 to to TO 10_1101-2021_01_07_425723 422 34 646 646 CD 10_1101-2021_01_07_425723 422 35 inoculate inoculate VB 10_1101-2021_01_07_425723 422 36 fresh fresh JJ 10_1101-2021_01_07_425723 422 37 - - HYPH 10_1101-2021_01_07_425723 422 38 media medium NNS 10_1101-2021_01_07_425723 422 39 in in IN 10_1101-2021_01_07_425723 422 40 the the DT 10_1101-2021_01_07_425723 422 41 other other JJ 10_1101-2021_01_07_425723 422 42 Bactron Bactron NNP 10_1101-2021_01_07_425723 422 43 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 422 44 workstation workstation NN 10_1101-2021_01_07_425723 422 45 . . . 10_1101-2021_01_07_425723 423 1 Simultaneously simultaneously RB 10_1101-2021_01_07_425723 423 2 , , , 10_1101-2021_01_07_425723 423 3 the the DT 10_1101-2021_01_07_425723 423 4 original original JJ 10_1101-2021_01_07_425723 423 5 culture culture NN 10_1101-2021_01_07_425723 423 6 647 647 CD 10_1101-2021_01_07_425723 423 7 was be VBD 10_1101-2021_01_07_425723 423 8 used use VBN 10_1101-2021_01_07_425723 423 9 to to TO 10_1101-2021_01_07_425723 423 10 inoculate inoculate VB 10_1101-2021_01_07_425723 423 11 fresh fresh JJ 10_1101-2021_01_07_425723 423 12 media medium NNS 10_1101-2021_01_07_425723 423 13 in in IN 10_1101-2021_01_07_425723 423 14 the the DT 10_1101-2021_01_07_425723 423 15 same same JJ 10_1101-2021_01_07_425723 423 16 Bactron Bactron NNP 10_1101-2021_01_07_425723 423 17 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 423 18 workstation workstation NN 10_1101-2021_01_07_425723 423 19 , , , 10_1101-2021_01_07_425723 423 20 thereby thereby RB 10_1101-2021_01_07_425723 423 21 resulting result VBG 10_1101-2021_01_07_425723 423 22 in in IN 10_1101-2021_01_07_425723 423 23 4 4 CD 10_1101-2021_01_07_425723 423 24 648 648 CD 10_1101-2021_01_07_425723 423 25 parallel parallel JJ 10_1101-2021_01_07_425723 423 26 cultures culture NNS 10_1101-2021_01_07_425723 423 27 of of IN 10_1101-2021_01_07_425723 423 28 each each DT 10_1101-2021_01_07_425723 423 29 strain strain NN 10_1101-2021_01_07_425723 423 30 of of IN 10_1101-2021_01_07_425723 423 31 which which WDT 10_1101-2021_01_07_425723 423 32 halve halve NN 10_1101-2021_01_07_425723 423 33 were be VBD 10_1101-2021_01_07_425723 423 34 derived derive VBN 10_1101-2021_01_07_425723 423 35 from from IN 10_1101-2021_01_07_425723 423 36 the the DT 10_1101-2021_01_07_425723 423 37 other other JJ 10_1101-2021_01_07_425723 423 38 Bactron Bactron NNP 10_1101-2021_01_07_425723 423 39 anaerobic anaerobic VBD 10_1101-2021_01_07_425723 423 40 649 649 CD 10_1101-2021_01_07_425723 423 41 workstation workstation NN 10_1101-2021_01_07_425723 423 42 . . . 10_1101-2021_01_07_425723 424 1 650 650 CD 10_1101-2021_01_07_425723 424 2 Laboratory laboratory NN 10_1101-2021_01_07_425723 424 3 evolution evolution NN 10_1101-2021_01_07_425723 424 4 in in IN 10_1101-2021_01_07_425723 424 5 sequential sequential JJ 10_1101-2021_01_07_425723 424 6 batch batch NN 10_1101-2021_01_07_425723 424 7 reactors reactor NNS 10_1101-2021_01_07_425723 424 8 651 651 CD 10_1101-2021_01_07_425723 424 9 .CC .CC , 10_1101-2021_01_07_425723 424 10 - - HYPH 10_1101-2021_01_07_425723 424 11 BY by IN 10_1101-2021_01_07_425723 424 12 - - HYPH 10_1101-2021_01_07_425723 424 13 NC NC NNP 10_1101-2021_01_07_425723 424 14 - - HYPH 10_1101-2021_01_07_425723 424 15 ND ND NNP 10_1101-2021_01_07_425723 424 16 4.0 4.0 CD 10_1101-2021_01_07_425723 424 17 International International NNP 10_1101-2021_01_07_425723 424 18 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 424 19 under under IN 10_1101-2021_01_07_425723 424 20 a a DT 10_1101-2021_01_07_425723 424 21 ( ( -LRB- 10_1101-2021_01_07_425723 424 22 which which WDT 10_1101-2021_01_07_425723 424 23 was be VBD 10_1101-2021_01_07_425723 424 24 not not RB 10_1101-2021_01_07_425723 424 25 certified certify VBN 10_1101-2021_01_07_425723 424 26 by by IN 10_1101-2021_01_07_425723 424 27 peer peer NN 10_1101-2021_01_07_425723 424 28 review review NN 10_1101-2021_01_07_425723 424 29 ) ) -RRB- 10_1101-2021_01_07_425723 424 30 is be VBZ 10_1101-2021_01_07_425723 424 31 the the DT 10_1101-2021_01_07_425723 424 32 author author NN 10_1101-2021_01_07_425723 424 33 / / SYM 10_1101-2021_01_07_425723 424 34 funder funder NN 10_1101-2021_01_07_425723 424 35 , , , 10_1101-2021_01_07_425723 424 36 who who WP 10_1101-2021_01_07_425723 424 37 has have VBZ 10_1101-2021_01_07_425723 424 38 granted grant VBN 10_1101-2021_01_07_425723 424 39 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 424 40 a a DT 10_1101-2021_01_07_425723 424 41 license license NN 10_1101-2021_01_07_425723 424 42 to to TO 10_1101-2021_01_07_425723 424 43 display display VB 10_1101-2021_01_07_425723 424 44 the the DT 10_1101-2021_01_07_425723 424 45 preprint preprint NN 10_1101-2021_01_07_425723 424 46 in in IN 10_1101-2021_01_07_425723 424 47 perpetuity perpetuity NN 10_1101-2021_01_07_425723 424 48 . . . 10_1101-2021_01_07_425723 425 1 It -PRON- PRP 10_1101-2021_01_07_425723 425 2 is be VBZ 10_1101-2021_01_07_425723 425 3 made make VBN 10_1101-2021_01_07_425723 425 4 The the DT 10_1101-2021_01_07_425723 425 5 copyright copyright NN 10_1101-2021_01_07_425723 425 6 holder holder NN 10_1101-2021_01_07_425723 425 7 for for IN 10_1101-2021_01_07_425723 425 8 this this DT 10_1101-2021_01_07_425723 425 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 425 10 version version NN 10_1101-2021_01_07_425723 425 11 posted post VBD 10_1101-2021_01_07_425723 425 12 January January NNP 10_1101-2021_01_07_425723 425 13 8 8 CD 10_1101-2021_01_07_425723 425 14 , , , 10_1101-2021_01_07_425723 425 15 2021 2021 CD 10_1101-2021_01_07_425723 425 16 . . . 10_1101-2021_01_07_425723 425 17 ; ; : 10_1101-2021_01_07_425723 425 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 425 19 : : : 10_1101-2021_01_07_425723 425 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 425 21 preprint preprint NN 10_1101-2021_01_07_425723 425 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 425 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425723 425 24 37 37 CD 10_1101-2021_01_07_425723 425 25 Laboratory Laboratory NNP 10_1101-2021_01_07_425723 425 26 evolution evolution NN 10_1101-2021_01_07_425723 425 27 for for IN 10_1101-2021_01_07_425723 425 28 selection selection NN 10_1101-2021_01_07_425723 425 29 of of IN 10_1101-2021_01_07_425723 425 30 fast fast JJ 10_1101-2021_01_07_425723 425 31 growth growth NN 10_1101-2021_01_07_425723 425 32 at at IN 10_1101-2021_01_07_425723 425 33 high high JJ 10_1101-2021_01_07_425723 425 34 temperatures temperature NNS 10_1101-2021_01_07_425723 425 35 was be VBD 10_1101-2021_01_07_425723 425 36 performed perform VBN 10_1101-2021_01_07_425723 425 37 in in IN 10_1101-2021_01_07_425723 425 38 400-mL 400-ml CD 10_1101-2021_01_07_425723 425 39 652 652 CD 10_1101-2021_01_07_425723 425 40 MultiFors MultiFors NNP 10_1101-2021_01_07_425723 425 41 ( ( -LRB- 10_1101-2021_01_07_425723 425 42 Infors Infors NNPS 10_1101-2021_01_07_425723 425 43 Benelux Benelux NNP 10_1101-2021_01_07_425723 425 44 , , , 10_1101-2021_01_07_425723 425 45 Velp Velp NNP 10_1101-2021_01_07_425723 425 46 , , , 10_1101-2021_01_07_425723 425 47 the the DT 10_1101-2021_01_07_425723 425 48 Netherlands Netherlands NNP 10_1101-2021_01_07_425723 425 49 ) ) -RRB- 10_1101-2021_01_07_425723 425 50 bioreactors bioreactor NNS 10_1101-2021_01_07_425723 425 51 with with IN 10_1101-2021_01_07_425723 425 52 a a DT 10_1101-2021_01_07_425723 425 53 working work VBG 10_1101-2021_01_07_425723 425 54 volume volume NN 10_1101-2021_01_07_425723 425 55 of of IN 10_1101-2021_01_07_425723 425 56 100 100 CD 10_1101-2021_01_07_425723 425 57 mL ml NN 10_1101-2021_01_07_425723 425 58 for for IN 10_1101-2021_01_07_425723 425 59 the the DT 10_1101-2021_01_07_425723 425 60 653 653 CD 10_1101-2021_01_07_425723 425 61 strain strain NN 10_1101-2021_01_07_425723 425 62 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 425 63 on on IN 10_1101-2021_01_07_425723 425 64 SMG SMG NNP 10_1101-2021_01_07_425723 425 65 20 20 CD 10_1101-2021_01_07_425723 425 66 g·L-1 g·l-1 CD 10_1101-2021_01_07_425723 425 67 glucose glucose VBP 10_1101-2021_01_07_425723 425 68 media medium NNS 10_1101-2021_01_07_425723 425 69 with with IN 10_1101-2021_01_07_425723 425 70 Tween Tween NNP 10_1101-2021_01_07_425723 425 71 80 80 CD 10_1101-2021_01_07_425723 425 72 in in IN 10_1101-2021_01_07_425723 425 73 triplicate triplicate NN 10_1101-2021_01_07_425723 425 74 . . . 10_1101-2021_01_07_425723 426 1 Anaerobic anaerobic JJ 10_1101-2021_01_07_425723 426 2 conditions condition NNS 10_1101-2021_01_07_425723 426 3 were be VBD 10_1101-2021_01_07_425723 426 4 654 654 CD 10_1101-2021_01_07_425723 426 5 created create VBN 10_1101-2021_01_07_425723 426 6 and and CC 10_1101-2021_01_07_425723 426 7 maintained maintain VBN 10_1101-2021_01_07_425723 426 8 by by IN 10_1101-2021_01_07_425723 426 9 continuous continuous JJ 10_1101-2021_01_07_425723 426 10 aeration aeration NN 10_1101-2021_01_07_425723 426 11 of of IN 10_1101-2021_01_07_425723 426 12 the the DT 10_1101-2021_01_07_425723 426 13 cultures culture NNS 10_1101-2021_01_07_425723 426 14 with with IN 10_1101-2021_01_07_425723 426 15 50 50 CD 10_1101-2021_01_07_425723 426 16 mL·min-1 mL·min-1 NNS 10_1101-2021_01_07_425723 426 17 ( ( -LRB- 10_1101-2021_01_07_425723 426 18 0.5 0.5 CD 10_1101-2021_01_07_425723 426 19 vvm vvm NN 10_1101-2021_01_07_425723 426 20 ) ) -RRB- 10_1101-2021_01_07_425723 426 21 N2 N2 NNP 10_1101-2021_01_07_425723 426 22 gas gas NN 10_1101-2021_01_07_425723 426 23 and and CC 10_1101-2021_01_07_425723 426 24 655 655 CD 10_1101-2021_01_07_425723 426 25 continuous continuous JJ 10_1101-2021_01_07_425723 426 26 aeration aeration NN 10_1101-2021_01_07_425723 426 27 of of IN 10_1101-2021_01_07_425723 426 28 the the DT 10_1101-2021_01_07_425723 426 29 media medium NNS 10_1101-2021_01_07_425723 426 30 vessels vessel NNS 10_1101-2021_01_07_425723 426 31 with with IN 10_1101-2021_01_07_425723 426 32 N2 N2 NNP 10_1101-2021_01_07_425723 426 33 gas gas NN 10_1101-2021_01_07_425723 426 34 . . . 10_1101-2021_01_07_425723 427 1 The the DT 10_1101-2021_01_07_425723 427 2 pH pH NNP 10_1101-2021_01_07_425723 427 3 was be VBD 10_1101-2021_01_07_425723 427 4 set set VBN 10_1101-2021_01_07_425723 427 5 at at IN 10_1101-2021_01_07_425723 427 6 5.0 5.0 CD 10_1101-2021_01_07_425723 427 7 and and CC 10_1101-2021_01_07_425723 427 8 maintained maintain VBN 10_1101-2021_01_07_425723 427 9 by by IN 10_1101-2021_01_07_425723 427 10 the the DT 10_1101-2021_01_07_425723 427 11 656 656 CD 10_1101-2021_01_07_425723 427 12 continuous continuous JJ 10_1101-2021_01_07_425723 427 13 addition addition NN 10_1101-2021_01_07_425723 427 14 of of IN 10_1101-2021_01_07_425723 427 15 sterile sterile JJ 10_1101-2021_01_07_425723 427 16 2 2 CD 10_1101-2021_01_07_425723 427 17 M M NNP 10_1101-2021_01_07_425723 427 18 KOH KOH NNP 10_1101-2021_01_07_425723 427 19 . . . 10_1101-2021_01_07_425723 428 1 Growth growth NN 10_1101-2021_01_07_425723 428 2 was be VBD 10_1101-2021_01_07_425723 428 3 monitored monitor VBN 10_1101-2021_01_07_425723 428 4 by by IN 10_1101-2021_01_07_425723 428 5 analysis analysis NN 10_1101-2021_01_07_425723 428 6 of of IN 10_1101-2021_01_07_425723 428 7 the the DT 10_1101-2021_01_07_425723 428 8 CO2 CO2 NNP 10_1101-2021_01_07_425723 428 9 in in IN 10_1101-2021_01_07_425723 428 10 the the DT 10_1101-2021_01_07_425723 428 11 bioreactor bioreactor NN 10_1101-2021_01_07_425723 428 12 657 657 CD 10_1101-2021_01_07_425723 428 13 off off IN 10_1101-2021_01_07_425723 428 14 - - HYPH 10_1101-2021_01_07_425723 428 15 gas gas NN 10_1101-2021_01_07_425723 428 16 and and CC 10_1101-2021_01_07_425723 428 17 a a DT 10_1101-2021_01_07_425723 428 18 new new JJ 10_1101-2021_01_07_425723 428 19 empty empty JJ 10_1101-2021_01_07_425723 428 20 - - HYPH 10_1101-2021_01_07_425723 428 21 refill refill NN 10_1101-2021_01_07_425723 428 22 cycle cycle NN 10_1101-2021_01_07_425723 428 23 was be VBD 10_1101-2021_01_07_425723 428 24 initiated initiate VBN 10_1101-2021_01_07_425723 428 25 when when WRB 10_1101-2021_01_07_425723 428 26 the the DT 10_1101-2021_01_07_425723 428 27 batch batch NN 10_1101-2021_01_07_425723 428 28 time time NN 10_1101-2021_01_07_425723 428 29 had have VBD 10_1101-2021_01_07_425723 428 30 at at IN 10_1101-2021_01_07_425723 428 31 least least JJS 10_1101-2021_01_07_425723 428 32 elapsed elapse VBN 10_1101-2021_01_07_425723 428 33 15 15 CD 10_1101-2021_01_07_425723 428 34 hours hour NNS 10_1101-2021_01_07_425723 428 35 and and CC 10_1101-2021_01_07_425723 428 36 658 658 CD 10_1101-2021_01_07_425723 428 37 the the DT 10_1101-2021_01_07_425723 428 38 CO2 CO2 NNP 10_1101-2021_01_07_425723 428 39 signal signal NN 10_1101-2021_01_07_425723 428 40 dropped drop VBD 10_1101-2021_01_07_425723 428 41 to to IN 10_1101-2021_01_07_425723 428 42 70 70 CD 10_1101-2021_01_07_425723 428 43 % % NN 10_1101-2021_01_07_425723 428 44 of of IN 10_1101-2021_01_07_425723 428 45 the the DT 10_1101-2021_01_07_425723 428 46 maximum maximum NN 10_1101-2021_01_07_425723 428 47 reached reach VBN 10_1101-2021_01_07_425723 428 48 in in IN 10_1101-2021_01_07_425723 428 49 each each DT 10_1101-2021_01_07_425723 428 50 batch batch NN 10_1101-2021_01_07_425723 428 51 . . . 10_1101-2021_01_07_425723 429 1 The the DT 10_1101-2021_01_07_425723 429 2 dilution dilution NN 10_1101-2021_01_07_425723 429 3 factor factor NN 10_1101-2021_01_07_425723 429 4 of of IN 10_1101-2021_01_07_425723 429 5 each each DT 10_1101-2021_01_07_425723 429 6 659 659 CD 10_1101-2021_01_07_425723 429 7 empty empty JJ 10_1101-2021_01_07_425723 429 8 - - HYPH 10_1101-2021_01_07_425723 429 9 refill refill NN 10_1101-2021_01_07_425723 429 10 cycle cycle NN 10_1101-2021_01_07_425723 429 11 was be VBD 10_1101-2021_01_07_425723 429 12 14.3-fold 14.3-fold CD 10_1101-2021_01_07_425723 429 13 ( ( -LRB- 10_1101-2021_01_07_425723 429 14 100 100 CD 10_1101-2021_01_07_425723 429 15 mL mL NNP 10_1101-2021_01_07_425723 429 16 working working NN 10_1101-2021_01_07_425723 429 17 volume volume NN 10_1101-2021_01_07_425723 429 18 , , , 10_1101-2021_01_07_425723 429 19 7 7 CD 10_1101-2021_01_07_425723 429 20 mL mL NNP 10_1101-2021_01_07_425723 429 21 residual residual JJ 10_1101-2021_01_07_425723 429 22 volume volume NN 10_1101-2021_01_07_425723 429 23 ) ) -RRB- 10_1101-2021_01_07_425723 429 24 . . . 10_1101-2021_01_07_425723 430 1 The the DT 10_1101-2021_01_07_425723 430 2 first first JJ 10_1101-2021_01_07_425723 430 3 batch batch NN 10_1101-2021_01_07_425723 430 4 660 660 CD 10_1101-2021_01_07_425723 430 5 fermentation fermentation NN 10_1101-2021_01_07_425723 430 6 was be VBD 10_1101-2021_01_07_425723 430 7 performed perform VBN 10_1101-2021_01_07_425723 430 8 at at IN 10_1101-2021_01_07_425723 430 9 30 30 CD 10_1101-2021_01_07_425723 430 10 ° ° , 10_1101-2021_01_07_425723 430 11 C C NNP 10_1101-2021_01_07_425723 430 12 after after IN 10_1101-2021_01_07_425723 430 13 which which WDT 10_1101-2021_01_07_425723 430 14 in in IN 10_1101-2021_01_07_425723 430 15 the the DT 10_1101-2021_01_07_425723 430 16 second second JJ 10_1101-2021_01_07_425723 430 17 batch batch NN 10_1101-2021_01_07_425723 430 18 the the DT 10_1101-2021_01_07_425723 430 19 temperature temperature NN 10_1101-2021_01_07_425723 430 20 was be VBD 10_1101-2021_01_07_425723 430 21 increased increase VBN 10_1101-2021_01_07_425723 430 22 to to IN 10_1101-2021_01_07_425723 430 23 661 661 CD 10_1101-2021_01_07_425723 430 24 42 42 CD 10_1101-2021_01_07_425723 430 25 ° ° NNS 10_1101-2021_01_07_425723 430 26 C C NNP 10_1101-2021_01_07_425723 430 27 and and CC 10_1101-2021_01_07_425723 430 28 maintained maintain VBD 10_1101-2021_01_07_425723 430 29 at at IN 10_1101-2021_01_07_425723 430 30 for for IN 10_1101-2021_01_07_425723 430 31 18 18 CD 10_1101-2021_01_07_425723 430 32 consecutive consecutive JJ 10_1101-2021_01_07_425723 430 33 sequential sequential JJ 10_1101-2021_01_07_425723 430 34 batches batch NNS 10_1101-2021_01_07_425723 430 35 . . . 10_1101-2021_01_07_425723 431 1 After after IN 10_1101-2021_01_07_425723 431 2 the the DT 10_1101-2021_01_07_425723 431 3 18 18 CD 10_1101-2021_01_07_425723 431 4 batch batch NN 10_1101-2021_01_07_425723 431 5 cycle cycle NN 10_1101-2021_01_07_425723 431 6 at at IN 10_1101-2021_01_07_425723 431 7 42 42 CD 10_1101-2021_01_07_425723 431 8 ° ° , 10_1101-2021_01_07_425723 431 9 C C NNP 10_1101-2021_01_07_425723 431 10 the the DT 10_1101-2021_01_07_425723 431 11 662 662 CD 10_1101-2021_01_07_425723 431 12 culture culture NN 10_1101-2021_01_07_425723 431 13 temperature temperature NN 10_1101-2021_01_07_425723 431 14 was be VBD 10_1101-2021_01_07_425723 431 15 again again RB 10_1101-2021_01_07_425723 431 16 increased increase VBN 10_1101-2021_01_07_425723 431 17 to to IN 10_1101-2021_01_07_425723 431 18 45 45 CD 10_1101-2021_01_07_425723 431 19 ° ° NNS 10_1101-2021_01_07_425723 431 20 C C NNP 10_1101-2021_01_07_425723 431 21 and and CC 10_1101-2021_01_07_425723 431 22 maintained maintain VBD 10_1101-2021_01_07_425723 431 23 subsequently subsequently RB 10_1101-2021_01_07_425723 431 24 . . . 10_1101-2021_01_07_425723 432 1 Growth growth NN 10_1101-2021_01_07_425723 432 2 rate rate NN 10_1101-2021_01_07_425723 432 3 was be VBD 10_1101-2021_01_07_425723 432 4 663 663 CD 10_1101-2021_01_07_425723 432 5 calculated calculate VBN 10_1101-2021_01_07_425723 432 6 based base VBN 10_1101-2021_01_07_425723 432 7 on on IN 10_1101-2021_01_07_425723 432 8 the the DT 10_1101-2021_01_07_425723 432 9 CO2 CO2 NNP 10_1101-2021_01_07_425723 432 10 production production NN 10_1101-2021_01_07_425723 432 11 as as IN 10_1101-2021_01_07_425723 432 12 measured measure VBN 10_1101-2021_01_07_425723 432 13 by by IN 10_1101-2021_01_07_425723 432 14 the the DT 10_1101-2021_01_07_425723 432 15 CO2 CO2 NNP 10_1101-2021_01_07_425723 432 16 fraction fraction NN 10_1101-2021_01_07_425723 432 17 in in IN 10_1101-2021_01_07_425723 432 18 the the DT 10_1101-2021_01_07_425723 432 19 culture culture NN 10_1101-2021_01_07_425723 432 20 off off IN 10_1101-2021_01_07_425723 432 21 - - HYPH 10_1101-2021_01_07_425723 432 22 gas gas NN 10_1101-2021_01_07_425723 432 23 in in IN 10_1101-2021_01_07_425723 432 24 664 664 CD 10_1101-2021_01_07_425723 432 25 essence essence NN 10_1101-2021_01_07_425723 432 26 as as IN 10_1101-2021_01_07_425723 432 27 described describe VBN 10_1101-2021_01_07_425723 432 28 previously83 previously83 NNP 10_1101-2021_01_07_425723 432 29 . . . 10_1101-2021_01_07_425723 433 1 In in IN 10_1101-2021_01_07_425723 433 2 short short JJ 10_1101-2021_01_07_425723 433 3 , , , 10_1101-2021_01_07_425723 433 4 the the DT 10_1101-2021_01_07_425723 433 5 CO2 CO2 NNP 10_1101-2021_01_07_425723 433 6 fraction fraction NN 10_1101-2021_01_07_425723 433 7 in in IN 10_1101-2021_01_07_425723 433 8 the the DT 10_1101-2021_01_07_425723 433 9 off off JJ 10_1101-2021_01_07_425723 433 10 - - HYPH 10_1101-2021_01_07_425723 433 11 gas gas NN 10_1101-2021_01_07_425723 433 12 was be VBD 10_1101-2021_01_07_425723 433 13 converted convert VBN 10_1101-2021_01_07_425723 433 14 to to IN 10_1101-2021_01_07_425723 433 15 a a DT 10_1101-2021_01_07_425723 433 16 CO2 CO2 NNP 10_1101-2021_01_07_425723 433 17 665 665 CD 10_1101-2021_01_07_425723 433 18 evolution evolution NN 10_1101-2021_01_07_425723 433 19 rate rate NN 10_1101-2021_01_07_425723 433 20 of of IN 10_1101-2021_01_07_425723 433 21 mmol mmol NN 10_1101-2021_01_07_425723 433 22 per per IN 10_1101-2021_01_07_425723 433 23 hour hour NN 10_1101-2021_01_07_425723 433 24 and and CC 10_1101-2021_01_07_425723 433 25 subsequently subsequently RB 10_1101-2021_01_07_425723 433 26 summed sum VBD 10_1101-2021_01_07_425723 433 27 over over IN 10_1101-2021_01_07_425723 433 28 time time NN 10_1101-2021_01_07_425723 433 29 for for IN 10_1101-2021_01_07_425723 433 30 each each DT 10_1101-2021_01_07_425723 433 31 cycle cycle NN 10_1101-2021_01_07_425723 433 32 . . . 10_1101-2021_01_07_425723 434 1 The the DT 10_1101-2021_01_07_425723 434 2 corresponding corresponding JJ 10_1101-2021_01_07_425723 434 3 666 666 CD 10_1101-2021_01_07_425723 434 4 cumulative cumulative JJ 10_1101-2021_01_07_425723 434 5 CO2 CO2 NNP 10_1101-2021_01_07_425723 434 6 profile profile NN 10_1101-2021_01_07_425723 434 7 was be VBD 10_1101-2021_01_07_425723 434 8 transformed transform VBN 10_1101-2021_01_07_425723 434 9 to to IN 10_1101-2021_01_07_425723 434 10 natural natural JJ 10_1101-2021_01_07_425723 434 11 log log NN 10_1101-2021_01_07_425723 434 12 after after IN 10_1101-2021_01_07_425723 434 13 which which WDT 10_1101-2021_01_07_425723 434 14 the the DT 10_1101-2021_01_07_425723 434 15 stepwise stepwise JJ 10_1101-2021_01_07_425723 434 16 slope slope NN 10_1101-2021_01_07_425723 434 17 of of IN 10_1101-2021_01_07_425723 434 18 the the DT 10_1101-2021_01_07_425723 434 19 log log NN 10_1101-2021_01_07_425723 434 20 667 667 CD 10_1101-2021_01_07_425723 434 21 transformed transform VBN 10_1101-2021_01_07_425723 434 22 data datum NNS 10_1101-2021_01_07_425723 434 23 was be VBD 10_1101-2021_01_07_425723 434 24 calculated calculate VBN 10_1101-2021_01_07_425723 434 25 . . . 10_1101-2021_01_07_425723 435 1 Subsequently subsequently RB 10_1101-2021_01_07_425723 435 2 an an DT 10_1101-2021_01_07_425723 435 3 iterative iterative JJ 10_1101-2021_01_07_425723 435 4 exclusion exclusion NN 10_1101-2021_01_07_425723 435 5 of of IN 10_1101-2021_01_07_425723 435 6 datapoints datapoint NNS 10_1101-2021_01_07_425723 435 7 of of IN 10_1101-2021_01_07_425723 435 8 the the DT 10_1101-2021_01_07_425723 435 9 stepwise stepwise JJ 10_1101-2021_01_07_425723 435 10 668 668 CD 10_1101-2021_01_07_425723 435 11 slope slope NN 10_1101-2021_01_07_425723 435 12 of of IN 10_1101-2021_01_07_425723 435 13 the the DT 10_1101-2021_01_07_425723 435 14 log log NN 10_1101-2021_01_07_425723 435 15 transformed transform VBN 10_1101-2021_01_07_425723 435 16 cumulative cumulative JJ 10_1101-2021_01_07_425723 435 17 CO2 CO2 NNP 10_1101-2021_01_07_425723 435 18 profile profile NN 10_1101-2021_01_07_425723 435 19 was be VBD 10_1101-2021_01_07_425723 435 20 performed perform VBN 10_1101-2021_01_07_425723 435 21 with with IN 10_1101-2021_01_07_425723 435 22 exclusion exclusion NN 10_1101-2021_01_07_425723 435 23 criteria criterion NNS 10_1101-2021_01_07_425723 435 24 of of IN 10_1101-2021_01_07_425723 435 25 more more JJR 10_1101-2021_01_07_425723 435 26 than than IN 10_1101-2021_01_07_425723 435 27 669 669 CD 10_1101-2021_01_07_425723 435 28 one one CD 10_1101-2021_01_07_425723 435 29 standard standard JJ 10_1101-2021_01_07_425723 435 30 deviation deviation NN 10_1101-2021_01_07_425723 435 31 below below IN 10_1101-2021_01_07_425723 435 32 the the DT 10_1101-2021_01_07_425723 435 33 mean mean NN 10_1101-2021_01_07_425723 435 34 . . . 10_1101-2021_01_07_425723 436 1 670 670 CD 10_1101-2021_01_07_425723 436 2 Variant variant JJ 10_1101-2021_01_07_425723 436 3 calling call VBG 10_1101-2021_01_07_425723 436 4 671 671 CD 10_1101-2021_01_07_425723 436 5 DNA dna NN 10_1101-2021_01_07_425723 436 6 sequencing sequencing NN 10_1101-2021_01_07_425723 436 7 reads read NNS 10_1101-2021_01_07_425723 436 8 were be VBD 10_1101-2021_01_07_425723 436 9 aligned align VBN 10_1101-2021_01_07_425723 436 10 into into IN 10_1101-2021_01_07_425723 436 11 the the DT 10_1101-2021_01_07_425723 436 12 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 436 13 described describe VBN 10_1101-2021_01_07_425723 436 14 above above IN 10_1101-2021_01_07_425723 436 15 including include VBG 10_1101-2021_01_07_425723 436 16 an an DT 10_1101-2021_01_07_425723 436 17 additional additional JJ 10_1101-2021_01_07_425723 436 18 672 672 CD 10_1101-2021_01_07_425723 436 19 sequence sequence NN 10_1101-2021_01_07_425723 436 20 with with IN 10_1101-2021_01_07_425723 436 21 TtSTC1 ttstc1 JJ 10_1101-2021_01_07_425723 436 22 construct construct NN 10_1101-2021_01_07_425723 436 23 , , , 10_1101-2021_01_07_425723 436 24 and and CC 10_1101-2021_01_07_425723 436 25 used use VBD 10_1101-2021_01_07_425723 436 26 to to TO 10_1101-2021_01_07_425723 436 27 detect detect VB 10_1101-2021_01_07_425723 436 28 sequence sequence NN 10_1101-2021_01_07_425723 436 29 variants variant NNS 10_1101-2021_01_07_425723 436 30 using use VBG 10_1101-2021_01_07_425723 436 31 a a DT 10_1101-2021_01_07_425723 436 32 method method NN 10_1101-2021_01_07_425723 436 33 previously previously RB 10_1101-2021_01_07_425723 436 34 673 673 CD 10_1101-2021_01_07_425723 436 35 reported84 reported84 NN 10_1101-2021_01_07_425723 436 36 . . . 10_1101-2021_01_07_425723 437 1 Briefly briefly RB 10_1101-2021_01_07_425723 437 2 , , , 10_1101-2021_01_07_425723 437 3 reads read NNS 10_1101-2021_01_07_425723 437 4 were be VBD 10_1101-2021_01_07_425723 437 5 aligned align VBN 10_1101-2021_01_07_425723 437 6 using use VBG 10_1101-2021_01_07_425723 437 7 BWA BWA NNP 10_1101-2021_01_07_425723 437 8 ( ( -LRB- 10_1101-2021_01_07_425723 437 9 v0.7.15-r1142-dirty)85 v0.7.15-r1142-dirty)85 NN 10_1101-2021_01_07_425723 437 10 , , , 10_1101-2021_01_07_425723 437 11 alignments alignment NNS 10_1101-2021_01_07_425723 437 12 were be VBD 10_1101-2021_01_07_425723 437 13 processed process VBN 10_1101-2021_01_07_425723 437 14 674 674 CD 10_1101-2021_01_07_425723 437 15 .CC .CC , 10_1101-2021_01_07_425723 437 16 - - HYPH 10_1101-2021_01_07_425723 437 17 BY by IN 10_1101-2021_01_07_425723 437 18 - - HYPH 10_1101-2021_01_07_425723 437 19 NC NC NNP 10_1101-2021_01_07_425723 437 20 - - HYPH 10_1101-2021_01_07_425723 437 21 ND ND NNP 10_1101-2021_01_07_425723 437 22 4.0 4.0 CD 10_1101-2021_01_07_425723 437 23 International International NNP 10_1101-2021_01_07_425723 437 24 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 437 25 under under IN 10_1101-2021_01_07_425723 437 26 a a DT 10_1101-2021_01_07_425723 437 27 ( ( -LRB- 10_1101-2021_01_07_425723 437 28 which which WDT 10_1101-2021_01_07_425723 437 29 was be VBD 10_1101-2021_01_07_425723 437 30 not not RB 10_1101-2021_01_07_425723 437 31 certified certify VBN 10_1101-2021_01_07_425723 437 32 by by IN 10_1101-2021_01_07_425723 437 33 peer peer NN 10_1101-2021_01_07_425723 437 34 review review NN 10_1101-2021_01_07_425723 437 35 ) ) -RRB- 10_1101-2021_01_07_425723 437 36 is be VBZ 10_1101-2021_01_07_425723 437 37 the the DT 10_1101-2021_01_07_425723 437 38 author author NN 10_1101-2021_01_07_425723 437 39 / / SYM 10_1101-2021_01_07_425723 437 40 funder funder NN 10_1101-2021_01_07_425723 437 41 , , , 10_1101-2021_01_07_425723 437 42 who who WP 10_1101-2021_01_07_425723 437 43 has have VBZ 10_1101-2021_01_07_425723 437 44 granted grant VBN 10_1101-2021_01_07_425723 437 45 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 437 46 a a DT 10_1101-2021_01_07_425723 437 47 license license NN 10_1101-2021_01_07_425723 437 48 to to TO 10_1101-2021_01_07_425723 437 49 display display VB 10_1101-2021_01_07_425723 437 50 the the DT 10_1101-2021_01_07_425723 437 51 preprint preprint NN 10_1101-2021_01_07_425723 437 52 in in IN 10_1101-2021_01_07_425723 437 53 perpetuity perpetuity NN 10_1101-2021_01_07_425723 437 54 . . . 10_1101-2021_01_07_425723 438 1 It -PRON- PRP 10_1101-2021_01_07_425723 438 2 is be VBZ 10_1101-2021_01_07_425723 438 3 made make VBN 10_1101-2021_01_07_425723 438 4 The the DT 10_1101-2021_01_07_425723 438 5 copyright copyright NN 10_1101-2021_01_07_425723 438 6 holder holder NN 10_1101-2021_01_07_425723 438 7 for for IN 10_1101-2021_01_07_425723 438 8 this this DT 10_1101-2021_01_07_425723 438 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 438 10 version version NN 10_1101-2021_01_07_425723 438 11 posted post VBD 10_1101-2021_01_07_425723 438 12 January January NNP 10_1101-2021_01_07_425723 438 13 8 8 CD 10_1101-2021_01_07_425723 438 14 , , , 10_1101-2021_01_07_425723 438 15 2021 2021 CD 10_1101-2021_01_07_425723 438 16 . . . 10_1101-2021_01_07_425723 438 17 ; ; : 10_1101-2021_01_07_425723 438 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 438 19 : : : 10_1101-2021_01_07_425723 438 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 438 21 preprint preprint NN 10_1101-2021_01_07_425723 438 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 438 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425723 438 24 38 38 CD 10_1101-2021_01_07_425723 438 25 using use VBG 10_1101-2021_01_07_425723 438 26 samtools samtool NNS 10_1101-2021_01_07_425723 438 27 ( ( -LRB- 10_1101-2021_01_07_425723 438 28 v1.3.1)77 v1.3.1)77 NNP 10_1101-2021_01_07_425723 438 29 and and CC 10_1101-2021_01_07_425723 438 30 Picard Picard NNP 10_1101-2021_01_07_425723 438 31 tools tool NNS 10_1101-2021_01_07_425723 438 32 ( ( -LRB- 10_1101-2021_01_07_425723 438 33 v2.20.2-SNAPSHOT v2.20.2-snapshot NN 10_1101-2021_01_07_425723 438 34 ) ) -RRB- 10_1101-2021_01_07_425723 438 35 ( ( -LRB- 10_1101-2021_01_07_425723 438 36 http://broadinstitute.github.io/picard http://broadinstitute.github.io/picard NN 10_1101-2021_01_07_425723 438 37 ) ) -RRB- 10_1101-2021_01_07_425723 438 38 , , , 10_1101-2021_01_07_425723 438 39 675 675 CD 10_1101-2021_01_07_425723 438 40 and and CC 10_1101-2021_01_07_425723 438 41 variants variant NNS 10_1101-2021_01_07_425723 438 42 were be VBD 10_1101-2021_01_07_425723 438 43 then then RB 10_1101-2021_01_07_425723 438 44 called call VBN 10_1101-2021_01_07_425723 438 45 using use VBG 10_1101-2021_01_07_425723 438 46 the the DT 10_1101-2021_01_07_425723 438 47 Genome Genome NNP 10_1101-2021_01_07_425723 438 48 Analysis Analysis NNP 10_1101-2021_01_07_425723 438 49 Toolkit Toolkit NNP 10_1101-2021_01_07_425723 438 50 ( ( -LRB- 10_1101-2021_01_07_425723 438 51 v3.8 v3.8 NNP 10_1101-2021_01_07_425723 438 52 - - HYPH 10_1101-2021_01_07_425723 438 53 1 1 CD 10_1101-2021_01_07_425723 438 54 - - HYPH 10_1101-2021_01_07_425723 438 55 0-gf15c1c3ef)86 0-gf15c1c3ef)86 CD 10_1101-2021_01_07_425723 438 56 HaplotypeCaller HaplotypeCaller NNP 10_1101-2021_01_07_425723 438 57 676 676 CD 10_1101-2021_01_07_425723 438 58 in in IN 10_1101-2021_01_07_425723 438 59 DISCOVERY DISCOVERY NNP 10_1101-2021_01_07_425723 438 60 and and CC 10_1101-2021_01_07_425723 438 61 GVCF GVCF NNP 10_1101-2021_01_07_425723 438 62 modes mode NNS 10_1101-2021_01_07_425723 438 63 . . . 10_1101-2021_01_07_425723 439 1 Variants variant NNS 10_1101-2021_01_07_425723 439 2 were be VBD 10_1101-2021_01_07_425723 439 3 only only RB 10_1101-2021_01_07_425723 439 4 called call VBN 10_1101-2021_01_07_425723 439 5 at at IN 10_1101-2021_01_07_425723 439 6 sites site NNS 10_1101-2021_01_07_425723 439 7 with with IN 10_1101-2021_01_07_425723 439 8 minimum minimum JJ 10_1101-2021_01_07_425723 439 9 variant variant JJ 10_1101-2021_01_07_425723 439 10 confidence confidence NN 10_1101-2021_01_07_425723 439 11 677 677 CD 10_1101-2021_01_07_425723 439 12 normalized normalize VBN 10_1101-2021_01_07_425723 439 13 by by IN 10_1101-2021_01_07_425723 439 14 unfiltered unfiltered JJ 10_1101-2021_01_07_425723 439 15 depth depth NN 10_1101-2021_01_07_425723 439 16 of of IN 10_1101-2021_01_07_425723 439 17 variant variant JJ 10_1101-2021_01_07_425723 439 18 samples sample NNS 10_1101-2021_01_07_425723 439 19 ( ( -LRB- 10_1101-2021_01_07_425723 439 20 QD QD NNP 10_1101-2021_01_07_425723 439 21 ) ) -RRB- 10_1101-2021_01_07_425723 439 22 of of IN 10_1101-2021_01_07_425723 439 23 20 20 CD 10_1101-2021_01_07_425723 439 24 , , , 10_1101-2021_01_07_425723 439 25 read read VBD 10_1101-2021_01_07_425723 439 26 depth depth NN 10_1101-2021_01_07_425723 439 27 ( ( -LRB- 10_1101-2021_01_07_425723 439 28 DP DP NNP 10_1101-2021_01_07_425723 439 29 ) ) -RRB- 10_1101-2021_01_07_425723 439 30 ≥ ≥ NN 10_1101-2021_01_07_425723 439 31 5 5 CD 10_1101-2021_01_07_425723 439 32 , , , 10_1101-2021_01_07_425723 439 33 and and CC 10_1101-2021_01_07_425723 439 34 genotype genotype NNP 10_1101-2021_01_07_425723 439 35 quality quality NN 10_1101-2021_01_07_425723 439 36 678 678 CD 10_1101-2021_01_07_425723 439 37 ( ( -LRB- 10_1101-2021_01_07_425723 439 38 GQ GQ NNP 10_1101-2021_01_07_425723 439 39 ) ) -RRB- 10_1101-2021_01_07_425723 439 40 > > XX 10_1101-2021_01_07_425723 439 41 20 20 CD 10_1101-2021_01_07_425723 439 42 , , , 10_1101-2021_01_07_425723 439 43 excluding exclude VBG 10_1101-2021_01_07_425723 439 44 a a DT 10_1101-2021_01_07_425723 439 45 7.1 7.1 CD 10_1101-2021_01_07_425723 439 46 kb kb NN 10_1101-2021_01_07_425723 439 47 region region NN 10_1101-2021_01_07_425723 439 48 in in IN 10_1101-2021_01_07_425723 439 49 chromosome chromosome NN 10_1101-2021_01_07_425723 439 50 5 5 CD 10_1101-2021_01_07_425723 439 51 containing contain VBG 10_1101-2021_01_07_425723 439 52 rDNA rdna NN 10_1101-2021_01_07_425723 439 53 . . . 10_1101-2021_01_07_425723 440 1 Variants variant NNS 10_1101-2021_01_07_425723 440 2 were be VBD 10_1101-2021_01_07_425723 440 3 annotated annotate VBN 10_1101-2021_01_07_425723 440 4 using use VBG 10_1101-2021_01_07_425723 440 5 679 679 CD 10_1101-2021_01_07_425723 440 6 the the DT 10_1101-2021_01_07_425723 440 7 genome genome JJ 10_1101-2021_01_07_425723 440 8 annotation annotation NN 10_1101-2021_01_07_425723 440 9 described describe VBN 10_1101-2021_01_07_425723 440 10 above above RB 10_1101-2021_01_07_425723 440 11 , , , 10_1101-2021_01_07_425723 440 12 including include VBG 10_1101-2021_01_07_425723 440 13 the the DT 10_1101-2021_01_07_425723 440 14 TtSTC1 ttstc1 NN 10_1101-2021_01_07_425723 440 15 construct construct NN 10_1101-2021_01_07_425723 440 16 , , , 10_1101-2021_01_07_425723 440 17 with with IN 10_1101-2021_01_07_425723 440 18 SnpEff SnpEff NNP 10_1101-2021_01_07_425723 440 19 ( ( -LRB- 10_1101-2021_01_07_425723 440 20 v5.0)87 v5.0)87 : 10_1101-2021_01_07_425723 440 21 and and CC 10_1101-2021_01_07_425723 440 22 680 680 CD 10_1101-2021_01_07_425723 440 23 VCFannotator VCFannotator NNP 10_1101-2021_01_07_425723 440 24 ( ( -LRB- 10_1101-2021_01_07_425723 440 25 http://vcfannotator.sourceforge.net http://vcfannotator.sourceforge.net NNP 10_1101-2021_01_07_425723 440 26 ) ) -RRB- 10_1101-2021_01_07_425723 440 27 . . . 10_1101-2021_01_07_425723 441 1 681 681 CD 10_1101-2021_01_07_425723 441 2 Statistics statistic NNS 10_1101-2021_01_07_425723 441 3 682 682 CD 10_1101-2021_01_07_425723 441 4 Statistical statistical JJ 10_1101-2021_01_07_425723 441 5 test test NN 10_1101-2021_01_07_425723 441 6 performed perform VBN 10_1101-2021_01_07_425723 441 7 are be VBP 10_1101-2021_01_07_425723 441 8 given give VBN 10_1101-2021_01_07_425723 441 9 as as IN 10_1101-2021_01_07_425723 441 10 two two CD 10_1101-2021_01_07_425723 441 11 sided side VBN 10_1101-2021_01_07_425723 441 12 with with IN 10_1101-2021_01_07_425723 441 13 unequal unequal JJ 10_1101-2021_01_07_425723 441 14 variance variance NN 10_1101-2021_01_07_425723 441 15 t t NN 10_1101-2021_01_07_425723 441 16 - - HYPH 10_1101-2021_01_07_425723 441 17 test test NN 10_1101-2021_01_07_425723 441 18 unless unless IN 10_1101-2021_01_07_425723 441 19 specifically specifically RB 10_1101-2021_01_07_425723 441 20 stated state VBN 10_1101-2021_01_07_425723 441 21 683 683 CD 10_1101-2021_01_07_425723 441 22 otherwise otherwise RB 10_1101-2021_01_07_425723 441 23 . . . 10_1101-2021_01_07_425723 442 1 We -PRON- PRP 10_1101-2021_01_07_425723 442 2 denote denote VBP 10_1101-2021_01_07_425723 442 3 technical technical JJ 10_1101-2021_01_07_425723 442 4 replicates replicate NNS 10_1101-2021_01_07_425723 442 5 as as IN 10_1101-2021_01_07_425723 442 6 measurements measurement NNS 10_1101-2021_01_07_425723 442 7 derived derive VBN 10_1101-2021_01_07_425723 442 8 from from IN 10_1101-2021_01_07_425723 442 9 a a DT 10_1101-2021_01_07_425723 442 10 single single JJ 10_1101-2021_01_07_425723 442 11 cell cell NN 10_1101-2021_01_07_425723 442 12 culture culture NN 10_1101-2021_01_07_425723 442 13 . . . 10_1101-2021_01_07_425723 443 1 Biological biological JJ 10_1101-2021_01_07_425723 443 2 684 684 CD 10_1101-2021_01_07_425723 443 3 replicates replicate NNS 10_1101-2021_01_07_425723 443 4 are be VBP 10_1101-2021_01_07_425723 443 5 measurements measurement NNS 10_1101-2021_01_07_425723 443 6 originating originate VBG 10_1101-2021_01_07_425723 443 7 from from IN 10_1101-2021_01_07_425723 443 8 independent independent JJ 10_1101-2021_01_07_425723 443 9 cell cell NN 10_1101-2021_01_07_425723 443 10 cultures culture NNS 10_1101-2021_01_07_425723 443 11 . . . 10_1101-2021_01_07_425723 444 1 Independent independent JJ 10_1101-2021_01_07_425723 444 2 experiments experiment NNS 10_1101-2021_01_07_425723 444 3 are be VBP 10_1101-2021_01_07_425723 444 4 685 685 CD 10_1101-2021_01_07_425723 444 5 two two CD 10_1101-2021_01_07_425723 444 6 experiments experiment NNS 10_1101-2021_01_07_425723 444 7 identical identical JJ 10_1101-2021_01_07_425723 444 8 in in IN 10_1101-2021_01_07_425723 444 9 set set VBN 10_1101-2021_01_07_425723 444 10 - - HYPH 10_1101-2021_01_07_425723 444 11 up up RP 10_1101-2021_01_07_425723 444 12 separated separate VBN 10_1101-2021_01_07_425723 444 13 by by IN 10_1101-2021_01_07_425723 444 14 the the DT 10_1101-2021_01_07_425723 444 15 difference difference NN 10_1101-2021_01_07_425723 444 16 in in IN 10_1101-2021_01_07_425723 444 17 execution execution NN 10_1101-2021_01_07_425723 444 18 days day NNS 10_1101-2021_01_07_425723 444 19 . . . 10_1101-2021_01_07_425723 445 1 If if IN 10_1101-2021_01_07_425723 445 2 possible possible JJ 10_1101-2021_01_07_425723 445 3 variance variance NN 10_1101-2021_01_07_425723 445 4 686 686 CD 10_1101-2021_01_07_425723 445 5 from from IN 10_1101-2021_01_07_425723 445 6 independent independent JJ 10_1101-2021_01_07_425723 445 7 experiments experiment NNS 10_1101-2021_01_07_425723 445 8 with with IN 10_1101-2021_01_07_425723 445 9 identical identical JJ 10_1101-2021_01_07_425723 445 10 setup setup NN 10_1101-2021_01_07_425723 445 11 were be VBD 10_1101-2021_01_07_425723 445 12 pooled pool VBN 10_1101-2021_01_07_425723 445 13 together together RB 10_1101-2021_01_07_425723 445 14 , , , 10_1101-2021_01_07_425723 445 15 but but CC 10_1101-2021_01_07_425723 445 16 independent independent JJ 10_1101-2021_01_07_425723 445 17 687 687 CD 10_1101-2021_01_07_425723 445 18 experiments experiment NNS 10_1101-2021_01_07_425723 445 19 from from IN 10_1101-2021_01_07_425723 445 20 time time NN 10_1101-2021_01_07_425723 445 21 - - HYPH 10_1101-2021_01_07_425723 445 22 course course NN 10_1101-2021_01_07_425723 445 23 experiments experiment NNS 10_1101-2021_01_07_425723 445 24 ( ( -LRB- 10_1101-2021_01_07_425723 445 25 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 445 26 growth growth NN 10_1101-2021_01_07_425723 445 27 studies study NNS 10_1101-2021_01_07_425723 445 28 ) ) -RRB- 10_1101-2021_01_07_425723 445 29 are be VBP 10_1101-2021_01_07_425723 445 30 reported report VBN 10_1101-2021_01_07_425723 445 31 separately separately RB 10_1101-2021_01_07_425723 445 32 . . . 10_1101-2021_01_07_425723 446 1 p-688 p-688 NNP 10_1101-2021_01_07_425723 446 2 values value NNS 10_1101-2021_01_07_425723 446 3 were be VBD 10_1101-2021_01_07_425723 446 4 corrected correct VBN 10_1101-2021_01_07_425723 446 5 for for IN 10_1101-2021_01_07_425723 446 6 multiple multiple JJ 10_1101-2021_01_07_425723 446 7 - - HYPH 10_1101-2021_01_07_425723 446 8 hypothesis hypothesis NN 10_1101-2021_01_07_425723 446 9 testing testing NN 10_1101-2021_01_07_425723 446 10 which which WDT 10_1101-2021_01_07_425723 446 11 is be VBZ 10_1101-2021_01_07_425723 446 12 specifically specifically RB 10_1101-2021_01_07_425723 446 13 reported report VBN 10_1101-2021_01_07_425723 446 14 each each DT 10_1101-2021_01_07_425723 446 15 time time NN 10_1101-2021_01_07_425723 446 16 . . . 10_1101-2021_01_07_425723 447 1 No no DT 10_1101-2021_01_07_425723 447 2 data data NN 10_1101-2021_01_07_425723 447 3 689 689 CD 10_1101-2021_01_07_425723 447 4 was be VBD 10_1101-2021_01_07_425723 447 5 excluded exclude VBN 10_1101-2021_01_07_425723 447 6 based base VBN 10_1101-2021_01_07_425723 447 7 on on IN 10_1101-2021_01_07_425723 447 8 the the DT 10_1101-2021_01_07_425723 447 9 resulting result VBG 10_1101-2021_01_07_425723 447 10 data datum NNS 10_1101-2021_01_07_425723 447 11 out out RB 10_1101-2021_01_07_425723 447 12 - - HYPH 10_1101-2021_01_07_425723 447 13 come come VB 10_1101-2021_01_07_425723 447 14 . . . 10_1101-2021_01_07_425723 448 1 690 690 CD 10_1101-2021_01_07_425723 448 2 Data datum NNS 10_1101-2021_01_07_425723 448 3 availability availability NN 10_1101-2021_01_07_425723 448 4 691 691 CD 10_1101-2021_01_07_425723 448 5 Data datum NNS 10_1101-2021_01_07_425723 448 6 supporting support VBG 10_1101-2021_01_07_425723 448 7 the the DT 10_1101-2021_01_07_425723 448 8 findings finding NNS 10_1101-2021_01_07_425723 448 9 of of IN 10_1101-2021_01_07_425723 448 10 this this DT 10_1101-2021_01_07_425723 448 11 work work NN 10_1101-2021_01_07_425723 448 12 are be VBP 10_1101-2021_01_07_425723 448 13 available available JJ 10_1101-2021_01_07_425723 448 14 within within IN 10_1101-2021_01_07_425723 448 15 the the DT 10_1101-2021_01_07_425723 448 16 paper paper NN 10_1101-2021_01_07_425723 448 17 and and CC 10_1101-2021_01_07_425723 448 18 source source NN 10_1101-2021_01_07_425723 448 19 data datum NNS 10_1101-2021_01_07_425723 448 20 for for IN 10_1101-2021_01_07_425723 448 21 all all DT 10_1101-2021_01_07_425723 448 22 figures figure NNS 10_1101-2021_01_07_425723 448 23 in in IN 10_1101-2021_01_07_425723 448 24 692 692 CD 10_1101-2021_01_07_425723 448 25 this this DT 10_1101-2021_01_07_425723 448 26 study study NN 10_1101-2021_01_07_425723 448 27 are be VBP 10_1101-2021_01_07_425723 448 28 available available JJ 10_1101-2021_01_07_425723 448 29 at at IN 10_1101-2021_01_07_425723 448 30 the the DT 10_1101-2021_01_07_425723 448 31 www.data.4TU.nl www.data.4TU.nl JJR 10_1101-2021_01_07_425723 448 32 repository repository NN 10_1101-2021_01_07_425723 448 33 with with IN 10_1101-2021_01_07_425723 448 34 the the DT 10_1101-2021_01_07_425723 448 35 doi:10.4121/13265552 doi:10.4121/13265552 NN 10_1101-2021_01_07_425723 448 36 . . . 10_1101-2021_01_07_425723 449 1 693 693 CD 10_1101-2021_01_07_425723 449 2 The the DT 10_1101-2021_01_07_425723 449 3 raw raw JJ 10_1101-2021_01_07_425723 449 4 RNA RNA NNP 10_1101-2021_01_07_425723 449 5 - - HYPH 10_1101-2021_01_07_425723 449 6 sequencing sequence VBG 10_1101-2021_01_07_425723 449 7 data datum NNS 10_1101-2021_01_07_425723 449 8 that that WDT 10_1101-2021_01_07_425723 449 9 supports support VBZ 10_1101-2021_01_07_425723 449 10 the the DT 10_1101-2021_01_07_425723 449 11 findings finding NNS 10_1101-2021_01_07_425723 449 12 of of IN 10_1101-2021_01_07_425723 449 13 this this DT 10_1101-2021_01_07_425723 449 14 study study NN 10_1101-2021_01_07_425723 449 15 are be VBP 10_1101-2021_01_07_425723 449 16 available available JJ 10_1101-2021_01_07_425723 449 17 from from IN 10_1101-2021_01_07_425723 449 18 the the DT 10_1101-2021_01_07_425723 449 19 Genome Genome NNP 10_1101-2021_01_07_425723 449 20 694 694 CD 10_1101-2021_01_07_425723 449 21 Expression Expression NNP 10_1101-2021_01_07_425723 449 22 Omnibus Omnibus NNP 10_1101-2021_01_07_425723 449 23 ( ( -LRB- 10_1101-2021_01_07_425723 449 24 GEO GEO NNP 10_1101-2021_01_07_425723 449 25 ) ) -RRB- 10_1101-2021_01_07_425723 449 26 website website NN 10_1101-2021_01_07_425723 449 27 ( ( -LRB- 10_1101-2021_01_07_425723 449 28 https://www.ncbi.nlm.nih.gov/geo/ https://www.ncbi.nlm.nih.gov/geo/ NNP 10_1101-2021_01_07_425723 449 29 ) ) -RRB- 10_1101-2021_01_07_425723 449 30 with with IN 10_1101-2021_01_07_425723 449 31 number number NN 10_1101-2021_01_07_425723 449 32 GSE164344 gse164344 NN 10_1101-2021_01_07_425723 449 33 . . . 10_1101-2021_01_07_425723 450 1 695 695 CD 10_1101-2021_01_07_425723 450 2 .CC .CC : 10_1101-2021_01_07_425723 450 3 - - HYPH 10_1101-2021_01_07_425723 450 4 BY by IN 10_1101-2021_01_07_425723 450 5 - - HYPH 10_1101-2021_01_07_425723 450 6 NC NC NNP 10_1101-2021_01_07_425723 450 7 - - HYPH 10_1101-2021_01_07_425723 450 8 ND ND NNP 10_1101-2021_01_07_425723 450 9 4.0 4.0 CD 10_1101-2021_01_07_425723 450 10 International International NNP 10_1101-2021_01_07_425723 450 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 450 12 under under IN 10_1101-2021_01_07_425723 450 13 a a DT 10_1101-2021_01_07_425723 450 14 ( ( -LRB- 10_1101-2021_01_07_425723 450 15 which which WDT 10_1101-2021_01_07_425723 450 16 was be VBD 10_1101-2021_01_07_425723 450 17 not not RB 10_1101-2021_01_07_425723 450 18 certified certify VBN 10_1101-2021_01_07_425723 450 19 by by IN 10_1101-2021_01_07_425723 450 20 peer peer NN 10_1101-2021_01_07_425723 450 21 review review NN 10_1101-2021_01_07_425723 450 22 ) ) -RRB- 10_1101-2021_01_07_425723 450 23 is be VBZ 10_1101-2021_01_07_425723 450 24 the the DT 10_1101-2021_01_07_425723 450 25 author author NN 10_1101-2021_01_07_425723 450 26 / / SYM 10_1101-2021_01_07_425723 450 27 funder funder NN 10_1101-2021_01_07_425723 450 28 , , , 10_1101-2021_01_07_425723 450 29 who who WP 10_1101-2021_01_07_425723 450 30 has have VBZ 10_1101-2021_01_07_425723 450 31 granted grant VBN 10_1101-2021_01_07_425723 450 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 450 33 a a DT 10_1101-2021_01_07_425723 450 34 license license NN 10_1101-2021_01_07_425723 450 35 to to TO 10_1101-2021_01_07_425723 450 36 display display VB 10_1101-2021_01_07_425723 450 37 the the DT 10_1101-2021_01_07_425723 450 38 preprint preprint NN 10_1101-2021_01_07_425723 450 39 in in IN 10_1101-2021_01_07_425723 450 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 450 41 . . . 10_1101-2021_01_07_425723 451 1 It -PRON- PRP 10_1101-2021_01_07_425723 451 2 is be VBZ 10_1101-2021_01_07_425723 451 3 made make VBN 10_1101-2021_01_07_425723 451 4 The the DT 10_1101-2021_01_07_425723 451 5 copyright copyright NN 10_1101-2021_01_07_425723 451 6 holder holder NN 10_1101-2021_01_07_425723 451 7 for for IN 10_1101-2021_01_07_425723 451 8 this this DT 10_1101-2021_01_07_425723 451 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 451 10 version version NN 10_1101-2021_01_07_425723 451 11 posted post VBD 10_1101-2021_01_07_425723 451 12 January January NNP 10_1101-2021_01_07_425723 451 13 8 8 CD 10_1101-2021_01_07_425723 451 14 , , , 10_1101-2021_01_07_425723 451 15 2021 2021 CD 10_1101-2021_01_07_425723 451 16 . . . 10_1101-2021_01_07_425723 451 17 ; ; : 10_1101-2021_01_07_425723 451 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 451 19 : : : 10_1101-2021_01_07_425723 451 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 451 21 preprint preprint NN 10_1101-2021_01_07_425723 451 22 http://www.data.4tu.nl/ http://www.data.4tu.nl/ '' 10_1101-2021_01_07_425723 451 23 https://www.ncbi.nlm.nih.gov/geo/ https://www.ncbi.nlm.nih.gov/geo/ NNP 10_1101-2021_01_07_425723 451 24 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 451 25 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CC 10_1101-2021_01_07_425723 451 26 39 39 CD 10_1101-2021_01_07_425723 451 27 Whole whole RB 10_1101-2021_01_07_425723 451 28 - - HYPH 10_1101-2021_01_07_425723 451 29 genome genome NN 10_1101-2021_01_07_425723 451 30 sequencing sequencing NN 10_1101-2021_01_07_425723 451 31 data datum NNS 10_1101-2021_01_07_425723 451 32 of of IN 10_1101-2021_01_07_425723 451 33 the the DT 10_1101-2021_01_07_425723 451 34 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 451 35 , , , 10_1101-2021_01_07_425723 451 36 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 451 37 and and CC 10_1101-2021_01_07_425723 451 38 evolved evolve VBD 10_1101-2021_01_07_425723 451 39 strains strain NNS 10_1101-2021_01_07_425723 451 40 were be VBD 10_1101-2021_01_07_425723 451 41 deposited deposit VBN 10_1101-2021_01_07_425723 451 42 at at IN 10_1101-2021_01_07_425723 451 43 NCBI NCBI NNP 10_1101-2021_01_07_425723 451 44 696 696 CD 10_1101-2021_01_07_425723 451 45 ( ( -LRB- 10_1101-2021_01_07_425723 451 46 https://www.ncbi.nlm.nih.gov/ https://www.ncbi.nlm.nih.gov/ NNP 10_1101-2021_01_07_425723 451 47 ) ) -RRB- 10_1101-2021_01_07_425723 451 48 under under IN 10_1101-2021_01_07_425723 451 49 BioProject BioProject NNP 10_1101-2021_01_07_425723 451 50 accession accession NN 10_1101-2021_01_07_425723 451 51 number number NN 10_1101-2021_01_07_425723 451 52 PRJNA679749 PRJNA679749 NNP 10_1101-2021_01_07_425723 451 53 . . . 10_1101-2021_01_07_425723 452 1 697 697 CD 10_1101-2021_01_07_425723 452 2 Code code NN 10_1101-2021_01_07_425723 452 3 availability availability NN 10_1101-2021_01_07_425723 452 4 698 698 CD 10_1101-2021_01_07_425723 452 5 The the DT 10_1101-2021_01_07_425723 452 6 code code NN 10_1101-2021_01_07_425723 452 7 that that WDT 10_1101-2021_01_07_425723 452 8 were be VBD 10_1101-2021_01_07_425723 452 9 used use VBN 10_1101-2021_01_07_425723 452 10 to to TO 10_1101-2021_01_07_425723 452 11 generate generate VB 10_1101-2021_01_07_425723 452 12 the the DT 10_1101-2021_01_07_425723 452 13 results result NNS 10_1101-2021_01_07_425723 452 14 obtained obtain VBN 10_1101-2021_01_07_425723 452 15 in in IN 10_1101-2021_01_07_425723 452 16 this this DT 10_1101-2021_01_07_425723 452 17 study study NN 10_1101-2021_01_07_425723 452 18 are be VBP 10_1101-2021_01_07_425723 452 19 archived archive VBN 10_1101-2021_01_07_425723 452 20 in in IN 10_1101-2021_01_07_425723 452 21 a a DT 10_1101-2021_01_07_425723 452 22 Gitlab Gitlab NNP 10_1101-2021_01_07_425723 452 23 699 699 CD 10_1101-2021_01_07_425723 452 24 repository repository NN 10_1101-2021_01_07_425723 452 25 ( ( -LRB- 10_1101-2021_01_07_425723 452 26 https://gitlab.tudelft.nl/rortizmerino/kmar_anaerobic https://gitlab.tudelft.nl/rortizmerino/kmar_anaerobic NN 10_1101-2021_01_07_425723 452 27 ) ) -RRB- 10_1101-2021_01_07_425723 452 28 . . . 10_1101-2021_01_07_425723 453 1 700 700 CD 10_1101-2021_01_07_425723 453 2 Author author NN 10_1101-2021_01_07_425723 453 3 ’s ’s POS 10_1101-2021_01_07_425723 453 4 contributions contribution NNS 10_1101-2021_01_07_425723 453 5 701 701 CD 10_1101-2021_01_07_425723 453 6 WD WD NNP 10_1101-2021_01_07_425723 453 7 and and CC 10_1101-2021_01_07_425723 453 8 JTP JTP NNP 10_1101-2021_01_07_425723 453 9 designed design VBD 10_1101-2021_01_07_425723 453 10 the the DT 10_1101-2021_01_07_425723 453 11 study study NN 10_1101-2021_01_07_425723 453 12 and and CC 10_1101-2021_01_07_425723 453 13 wrote write VBD 10_1101-2021_01_07_425723 453 14 the the DT 10_1101-2021_01_07_425723 453 15 manuscript manuscript NN 10_1101-2021_01_07_425723 453 16 . . . 10_1101-2021_01_07_425723 454 1 WD WD NNP 10_1101-2021_01_07_425723 454 2 performed perform VBD 10_1101-2021_01_07_425723 454 3 molecular molecular JJ 10_1101-2021_01_07_425723 454 4 cloning cloning NN 10_1101-2021_01_07_425723 454 5 , , , 10_1101-2021_01_07_425723 454 6 bioreactor bioreactor NN 10_1101-2021_01_07_425723 454 7 702 702 CD 10_1101-2021_01_07_425723 454 8 cultivation cultivation NN 10_1101-2021_01_07_425723 454 9 experiment experiment NN 10_1101-2021_01_07_425723 454 10 , , , 10_1101-2021_01_07_425723 454 11 transcriptome transcriptome DT 10_1101-2021_01_07_425723 454 12 analysis analysis NN 10_1101-2021_01_07_425723 454 13 and and CC 10_1101-2021_01_07_425723 454 14 sterol sterol NN 10_1101-2021_01_07_425723 454 15 - - HYPH 10_1101-2021_01_07_425723 454 16 uptake uptake JJ 10_1101-2021_01_07_425723 454 17 experiments experiment NNS 10_1101-2021_01_07_425723 454 18 . . . 10_1101-2021_01_07_425723 455 1 JB JB NNP 10_1101-2021_01_07_425723 455 2 contributed contribute VBD 10_1101-2021_01_07_425723 455 3 to to IN 10_1101-2021_01_07_425723 455 4 703 703 CD 10_1101-2021_01_07_425723 455 5 bioreactor bioreactor NN 10_1101-2021_01_07_425723 455 6 cultivation cultivation NN 10_1101-2021_01_07_425723 455 7 experiments experiment NNS 10_1101-2021_01_07_425723 455 8 and and CC 10_1101-2021_01_07_425723 455 9 molecular molecular JJ 10_1101-2021_01_07_425723 455 10 cloning cloning NN 10_1101-2021_01_07_425723 455 11 . . . 10_1101-2021_01_07_425723 456 1 FW FW NNP 10_1101-2021_01_07_425723 456 2 contributed contribute VBD 10_1101-2021_01_07_425723 456 3 to to IN 10_1101-2021_01_07_425723 456 4 the the DT 10_1101-2021_01_07_425723 456 5 molecular molecular JJ 10_1101-2021_01_07_425723 456 6 cloning cloning NN 10_1101-2021_01_07_425723 456 7 and and CC 10_1101-2021_01_07_425723 456 8 704 704 CD 10_1101-2021_01_07_425723 456 9 sterol sterol JJ 10_1101-2021_01_07_425723 456 10 - - HYPH 10_1101-2021_01_07_425723 456 11 uptake uptake NN 10_1101-2021_01_07_425723 456 12 experiments experiment NNS 10_1101-2021_01_07_425723 456 13 . . . 10_1101-2021_01_07_425723 457 1 AK AK NNP 10_1101-2021_01_07_425723 457 2 and and CC 10_1101-2021_01_07_425723 457 3 CM CM NNP 10_1101-2021_01_07_425723 457 4 contributed contribute VBD 10_1101-2021_01_07_425723 457 5 to to IN 10_1101-2021_01_07_425723 457 6 bioreactor bioreactor NN 10_1101-2021_01_07_425723 457 7 experiments experiment NNS 10_1101-2021_01_07_425723 457 8 and and CC 10_1101-2021_01_07_425723 457 9 transcriptome transcriptome DT 10_1101-2021_01_07_425723 457 10 705 705 CD 10_1101-2021_01_07_425723 457 11 studies study NNS 10_1101-2021_01_07_425723 457 12 . . . 10_1101-2021_01_07_425723 458 1 PdlT PdlT NNP 10_1101-2021_01_07_425723 458 2 performed perform VBD 10_1101-2021_01_07_425723 458 3 plasmid plasmid NN 10_1101-2021_01_07_425723 458 4 and and CC 10_1101-2021_01_07_425723 458 5 genome genome JJ 10_1101-2021_01_07_425723 458 6 sequencing sequencing NN 10_1101-2021_01_07_425723 458 7 . . . 10_1101-2021_01_07_425723 459 1 RO RO NNP 10_1101-2021_01_07_425723 459 2 contributed contribute VBD 10_1101-2021_01_07_425723 459 3 to to IN 10_1101-2021_01_07_425723 459 4 transcriptome transcriptome DT 10_1101-2021_01_07_425723 459 5 analysis analysis NN 10_1101-2021_01_07_425723 459 6 and and CC 10_1101-2021_01_07_425723 459 7 706 706 CD 10_1101-2021_01_07_425723 459 8 performed perform VBN 10_1101-2021_01_07_425723 459 9 sequence sequence NN 10_1101-2021_01_07_425723 459 10 annotation annotation NN 10_1101-2021_01_07_425723 459 11 and and CC 10_1101-2021_01_07_425723 459 12 assembly assembly NN 10_1101-2021_01_07_425723 459 13 . . . 10_1101-2021_01_07_425723 460 1 707 707 CD 10_1101-2021_01_07_425723 460 2 Acknowledgements acknowledgement NNS 10_1101-2021_01_07_425723 460 3 708 708 CD 10_1101-2021_01_07_425723 460 4 We -PRON- PRP 10_1101-2021_01_07_425723 460 5 thank thank VBP 10_1101-2021_01_07_425723 460 6 Mark Mark NNP 10_1101-2021_01_07_425723 460 7 Bisschops Bisschops NNP 10_1101-2021_01_07_425723 460 8 and and CC 10_1101-2021_01_07_425723 460 9 Hannes Hannes NNP 10_1101-2021_01_07_425723 460 10 Jürgens Jürgens NNP 10_1101-2021_01_07_425723 460 11 for for IN 10_1101-2021_01_07_425723 460 12 fruitful fruitful JJ 10_1101-2021_01_07_425723 460 13 discussions discussion NNS 10_1101-2021_01_07_425723 460 14 . . . 10_1101-2021_01_07_425723 461 1 We -PRON- PRP 10_1101-2021_01_07_425723 461 2 thank thank VBP 10_1101-2021_01_07_425723 461 3 Erik Erik NNP 10_1101-2021_01_07_425723 461 4 de de FW 10_1101-2021_01_07_425723 461 5 Hulster Hulster NNP 10_1101-2021_01_07_425723 461 6 for for IN 10_1101-2021_01_07_425723 461 7 709 709 CD 10_1101-2021_01_07_425723 461 8 fermentation fermentation NN 10_1101-2021_01_07_425723 461 9 support support NN 10_1101-2021_01_07_425723 461 10 and and CC 10_1101-2021_01_07_425723 461 11 Marcel Marcel NNP 10_1101-2021_01_07_425723 461 12 van van NNP 10_1101-2021_01_07_425723 461 13 den den NNP 10_1101-2021_01_07_425723 461 14 Broek Broek NNP 10_1101-2021_01_07_425723 461 15 for for IN 10_1101-2021_01_07_425723 461 16 input input NN 10_1101-2021_01_07_425723 461 17 on on IN 10_1101-2021_01_07_425723 461 18 the the DT 10_1101-2021_01_07_425723 461 19 bioinformatics bioinformatic NNS 10_1101-2021_01_07_425723 461 20 analyses analysis NNS 10_1101-2021_01_07_425723 461 21 . . . 10_1101-2021_01_07_425723 462 1 710 710 CD 10_1101-2021_01_07_425723 462 2 Competing compete VBG 10_1101-2021_01_07_425723 462 3 interest interest NN 10_1101-2021_01_07_425723 462 4 711 711 CD 10_1101-2021_01_07_425723 462 5 WD WD NNP 10_1101-2021_01_07_425723 462 6 and and CC 10_1101-2021_01_07_425723 462 7 JTP JTP NNP 10_1101-2021_01_07_425723 462 8 are be VBP 10_1101-2021_01_07_425723 462 9 co co NNS 10_1101-2021_01_07_425723 462 10 - - NNS 10_1101-2021_01_07_425723 462 11 inventors inventor NNS 10_1101-2021_01_07_425723 462 12 on on IN 10_1101-2021_01_07_425723 462 13 a a DT 10_1101-2021_01_07_425723 462 14 patent patent NN 10_1101-2021_01_07_425723 462 15 application application NN 10_1101-2021_01_07_425723 462 16 that that WDT 10_1101-2021_01_07_425723 462 17 covers cover VBZ 10_1101-2021_01_07_425723 462 18 aspects aspect NNS 10_1101-2021_01_07_425723 462 19 of of IN 10_1101-2021_01_07_425723 462 20 this this DT 10_1101-2021_01_07_425723 462 21 work work NN 10_1101-2021_01_07_425723 462 22 . . . 10_1101-2021_01_07_425723 463 1 The the DT 10_1101-2021_01_07_425723 463 2 authors author NNS 10_1101-2021_01_07_425723 463 3 712 712 CD 10_1101-2021_01_07_425723 463 4 declare declare VBP 10_1101-2021_01_07_425723 463 5 no no DT 10_1101-2021_01_07_425723 463 6 conflict conflict NN 10_1101-2021_01_07_425723 463 7 of of IN 10_1101-2021_01_07_425723 463 8 interest interest NN 10_1101-2021_01_07_425723 463 9 . . . 10_1101-2021_01_07_425723 464 1 713 713 CD 10_1101-2021_01_07_425723 464 2 Funding funding NN 10_1101-2021_01_07_425723 464 3 714 714 CD 10_1101-2021_01_07_425723 464 4 This this DT 10_1101-2021_01_07_425723 464 5 work work NN 10_1101-2021_01_07_425723 464 6 was be VBD 10_1101-2021_01_07_425723 464 7 supported support VBN 10_1101-2021_01_07_425723 464 8 by by IN 10_1101-2021_01_07_425723 464 9 Advanced Advanced NNP 10_1101-2021_01_07_425723 464 10 Grant Grant NNP 10_1101-2021_01_07_425723 464 11 ( ( -LRB- 10_1101-2021_01_07_425723 464 12 grant grant VB 10_1101-2021_01_07_425723 464 13 # # $ 10_1101-2021_01_07_425723 464 14 694633 694633 CD 10_1101-2021_01_07_425723 464 15 ) ) -RRB- 10_1101-2021_01_07_425723 464 16 of of IN 10_1101-2021_01_07_425723 464 17 the the DT 10_1101-2021_01_07_425723 464 18 European European NNP 10_1101-2021_01_07_425723 464 19 Research Research NNP 10_1101-2021_01_07_425723 464 20 Council Council NNP 10_1101-2021_01_07_425723 464 21 to to IN 10_1101-2021_01_07_425723 464 22 JTP JTP NNP 10_1101-2021_01_07_425723 464 23 . . . 10_1101-2021_01_07_425723 465 1 715 715 CD 10_1101-2021_01_07_425723 465 2 .CC .CC NFP 10_1101-2021_01_07_425723 465 3 - - HYPH 10_1101-2021_01_07_425723 465 4 BY by IN 10_1101-2021_01_07_425723 465 5 - - HYPH 10_1101-2021_01_07_425723 465 6 NC NC NNP 10_1101-2021_01_07_425723 465 7 - - HYPH 10_1101-2021_01_07_425723 465 8 ND ND NNP 10_1101-2021_01_07_425723 465 9 4.0 4.0 CD 10_1101-2021_01_07_425723 465 10 International International NNP 10_1101-2021_01_07_425723 465 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 465 12 under under IN 10_1101-2021_01_07_425723 465 13 a a DT 10_1101-2021_01_07_425723 465 14 ( ( -LRB- 10_1101-2021_01_07_425723 465 15 which which WDT 10_1101-2021_01_07_425723 465 16 was be VBD 10_1101-2021_01_07_425723 465 17 not not RB 10_1101-2021_01_07_425723 465 18 certified certify VBN 10_1101-2021_01_07_425723 465 19 by by IN 10_1101-2021_01_07_425723 465 20 peer peer NN 10_1101-2021_01_07_425723 465 21 review review NN 10_1101-2021_01_07_425723 465 22 ) ) -RRB- 10_1101-2021_01_07_425723 465 23 is be VBZ 10_1101-2021_01_07_425723 465 24 the the DT 10_1101-2021_01_07_425723 465 25 author author NN 10_1101-2021_01_07_425723 465 26 / / SYM 10_1101-2021_01_07_425723 465 27 funder funder NN 10_1101-2021_01_07_425723 465 28 , , , 10_1101-2021_01_07_425723 465 29 who who WP 10_1101-2021_01_07_425723 465 30 has have VBZ 10_1101-2021_01_07_425723 465 31 granted grant VBN 10_1101-2021_01_07_425723 465 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 465 33 a a DT 10_1101-2021_01_07_425723 465 34 license license NN 10_1101-2021_01_07_425723 465 35 to to TO 10_1101-2021_01_07_425723 465 36 display display VB 10_1101-2021_01_07_425723 465 37 the the DT 10_1101-2021_01_07_425723 465 38 preprint preprint NN 10_1101-2021_01_07_425723 465 39 in in IN 10_1101-2021_01_07_425723 465 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 465 41 . . . 10_1101-2021_01_07_425723 466 1 It -PRON- PRP 10_1101-2021_01_07_425723 466 2 is be VBZ 10_1101-2021_01_07_425723 466 3 made make VBN 10_1101-2021_01_07_425723 466 4 The the DT 10_1101-2021_01_07_425723 466 5 copyright copyright NN 10_1101-2021_01_07_425723 466 6 holder holder NN 10_1101-2021_01_07_425723 466 7 for for IN 10_1101-2021_01_07_425723 466 8 this this DT 10_1101-2021_01_07_425723 466 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 466 10 version version NN 10_1101-2021_01_07_425723 466 11 posted post VBD 10_1101-2021_01_07_425723 466 12 January January NNP 10_1101-2021_01_07_425723 466 13 8 8 CD 10_1101-2021_01_07_425723 466 14 , , , 10_1101-2021_01_07_425723 466 15 2021 2021 CD 10_1101-2021_01_07_425723 466 16 . . . 10_1101-2021_01_07_425723 466 17 ; ; : 10_1101-2021_01_07_425723 466 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 466 19 : : : 10_1101-2021_01_07_425723 466 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 466 21 preprint preprint NN 10_1101-2021_01_07_425723 466 22 https://gitlab.tudelft.nl/rortizmerino/kmar_anaerobic https://gitlab.tudelft.nl/rortizmerino/kmar_anaerobic -RRB- 10_1101-2021_01_07_425723 466 23 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 466 24 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425723 466 25 40 40 CD 10_1101-2021_01_07_425723 466 26 References reference NNS 10_1101-2021_01_07_425723 466 27 716 716 CD 10_1101-2021_01_07_425723 466 28 1 1 CD 10_1101-2021_01_07_425723 466 29 . . . 10_1101-2021_01_07_425723 467 1 Annual Annual NNP 10_1101-2021_01_07_425723 467 2 World World NNP 10_1101-2021_01_07_425723 467 3 Fuel Fuel NNP 10_1101-2021_01_07_425723 467 4 Ethanol Ethanol NNP 10_1101-2021_01_07_425723 467 5 Production Production NNP 10_1101-2021_01_07_425723 467 6 . . . 10_1101-2021_01_07_425723 468 1 Renewable renewable JJ 10_1101-2021_01_07_425723 468 2 Fuels Fuels NNPS 10_1101-2021_01_07_425723 468 3 Association Association NNP 10_1101-2021_01_07_425723 468 4 ( ( -LRB- 10_1101-2021_01_07_425723 468 5 2020 2020 CD 10_1101-2021_01_07_425723 468 6 ) ) -RRB- 10_1101-2021_01_07_425723 468 7 . . . 10_1101-2021_01_07_425723 469 1 Available available JJ 10_1101-2021_01_07_425723 469 2 at at IN 10_1101-2021_01_07_425723 469 3 : : : 10_1101-2021_01_07_425723 469 4 717 717 CD 10_1101-2021_01_07_425723 469 5 https://ethanolrfa.org/statistics/annual-ethanol-production/. https://ethanolrfa.org/statistics/annual-ethanol-production/. . 10_1101-2021_01_07_425723 470 1 ( ( -LRB- 10_1101-2021_01_07_425723 470 2 Accessed access VBN 10_1101-2021_01_07_425723 470 3 : : : 10_1101-2021_01_07_425723 470 4 2nd 2nd JJ 10_1101-2021_01_07_425723 470 5 May May NNP 10_1101-2021_01_07_425723 470 6 2020 2020 CD 10_1101-2021_01_07_425723 470 7 ) ) -RRB- 10_1101-2021_01_07_425723 470 8 718 718 CD 10_1101-2021_01_07_425723 470 9 2 2 CD 10_1101-2021_01_07_425723 470 10 . . . 10_1101-2021_01_07_425723 471 1 Jansen Jansen NNP 10_1101-2021_01_07_425723 471 2 , , , 10_1101-2021_01_07_425723 471 3 M. M. NNP 10_1101-2021_01_07_425723 471 4 L. L. NNP 10_1101-2021_01_07_425723 471 5 A. A. NNP 10_1101-2021_01_07_425723 471 6 et et FW 10_1101-2021_01_07_425723 471 7 al al NNP 10_1101-2021_01_07_425723 471 8 . . . 10_1101-2021_01_07_425723 472 1 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 472 2 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 472 3 strains strain VBZ 10_1101-2021_01_07_425723 472 4 for for IN 10_1101-2021_01_07_425723 472 5 second second JJ 10_1101-2021_01_07_425723 472 6 - - HYPH 10_1101-2021_01_07_425723 472 7 generation generation NN 10_1101-2021_01_07_425723 472 8 ethanol ethanol NN 10_1101-2021_01_07_425723 472 9 719 719 CD 10_1101-2021_01_07_425723 472 10 production production NN 10_1101-2021_01_07_425723 472 11 : : : 10_1101-2021_01_07_425723 472 12 from from IN 10_1101-2021_01_07_425723 472 13 academic academic JJ 10_1101-2021_01_07_425723 472 14 exploration exploration NN 10_1101-2021_01_07_425723 472 15 to to IN 10_1101-2021_01_07_425723 472 16 industrial industrial JJ 10_1101-2021_01_07_425723 472 17 implementation implementation NN 10_1101-2021_01_07_425723 472 18 . . . 10_1101-2021_01_07_425723 473 1 FEMS FEMS NNP 10_1101-2021_01_07_425723 473 2 Yeast Yeast NNP 10_1101-2021_01_07_425723 473 3 Res res NN 10_1101-2021_01_07_425723 473 4 . . . 10_1101-2021_01_07_425723 474 1 17 17 CD 10_1101-2021_01_07_425723 474 2 , , , 10_1101-2021_01_07_425723 474 3 1–20 1–20 CD 10_1101-2021_01_07_425723 474 4 720 720 CD 10_1101-2021_01_07_425723 474 5 ( ( -LRB- 10_1101-2021_01_07_425723 474 6 2017 2017 CD 10_1101-2021_01_07_425723 474 7 ) ) -RRB- 10_1101-2021_01_07_425723 474 8 . . . 10_1101-2021_01_07_425723 475 1 721 721 CD 10_1101-2021_01_07_425723 475 2 3 3 CD 10_1101-2021_01_07_425723 475 3 . . . 10_1101-2021_01_07_425723 476 1 Weusthuis Weusthuis NNP 10_1101-2021_01_07_425723 476 2 , , , 10_1101-2021_01_07_425723 476 3 R. R. NNP 10_1101-2021_01_07_425723 476 4 A. A. NNP 10_1101-2021_01_07_425723 476 5 , , , 10_1101-2021_01_07_425723 476 6 Lamot Lamot NNP 10_1101-2021_01_07_425723 476 7 , , , 10_1101-2021_01_07_425723 476 8 I. I. NNP 10_1101-2021_01_07_425723 476 9 , , , 10_1101-2021_01_07_425723 476 10 van van NNP 10_1101-2021_01_07_425723 476 11 der der NNP 10_1101-2021_01_07_425723 476 12 Oost Oost NNP 10_1101-2021_01_07_425723 476 13 , , , 10_1101-2021_01_07_425723 476 14 J. J. NNP 10_1101-2021_01_07_425723 477 1 & & CC 10_1101-2021_01_07_425723 477 2 Sanders Sanders NNP 10_1101-2021_01_07_425723 477 3 , , , 10_1101-2021_01_07_425723 477 4 J. J. NNP 10_1101-2021_01_07_425723 477 5 P. P. NNP 10_1101-2021_01_07_425723 477 6 M. M. NNP 10_1101-2021_01_07_425723 477 7 Microbial Microbial NNP 10_1101-2021_01_07_425723 477 8 production production NN 10_1101-2021_01_07_425723 477 9 of of IN 10_1101-2021_01_07_425723 477 10 bulk bulk NN 10_1101-2021_01_07_425723 477 11 722 722 CD 10_1101-2021_01_07_425723 477 12 chemicals chemical NNS 10_1101-2021_01_07_425723 477 13 : : : 10_1101-2021_01_07_425723 477 14 development development NN 10_1101-2021_01_07_425723 477 15 of of IN 10_1101-2021_01_07_425723 477 16 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 477 17 processes process NNS 10_1101-2021_01_07_425723 477 18 . . . 10_1101-2021_01_07_425723 478 1 Trends Trends NNPS 10_1101-2021_01_07_425723 478 2 Biotechnol Biotechnol NNP 10_1101-2021_01_07_425723 478 3 . . . 10_1101-2021_01_07_425723 479 1 29 29 CD 10_1101-2021_01_07_425723 479 2 , , , 10_1101-2021_01_07_425723 479 3 153–158 153–158 CD 10_1101-2021_01_07_425723 479 4 ( ( -LRB- 10_1101-2021_01_07_425723 479 5 2011 2011 CD 10_1101-2021_01_07_425723 479 6 ) ) -RRB- 10_1101-2021_01_07_425723 479 7 . . . 10_1101-2021_01_07_425723 480 1 723 723 CD 10_1101-2021_01_07_425723 480 2 4 4 CD 10_1101-2021_01_07_425723 480 3 . . . 10_1101-2021_01_07_425723 481 1 Favaro Favaro NNP 10_1101-2021_01_07_425723 481 2 , , , 10_1101-2021_01_07_425723 481 3 L. L. NNP 10_1101-2021_01_07_425723 481 4 , , , 10_1101-2021_01_07_425723 481 5 Jansen Jansen NNP 10_1101-2021_01_07_425723 481 6 , , , 10_1101-2021_01_07_425723 481 7 T. T. NNP 10_1101-2021_01_07_425723 481 8 & & CC 10_1101-2021_01_07_425723 481 9 van van NNP 10_1101-2021_01_07_425723 481 10 Zyl Zyl NNP 10_1101-2021_01_07_425723 481 11 , , , 10_1101-2021_01_07_425723 481 12 W. W. NNP 10_1101-2021_01_07_425723 481 13 H. H. NNP 10_1101-2021_01_07_425723 481 14 Exploring Exploring NNP 10_1101-2021_01_07_425723 481 15 industrial industrial JJ 10_1101-2021_01_07_425723 481 16 and and CC 10_1101-2021_01_07_425723 481 17 natural natural JJ 10_1101-2021_01_07_425723 481 18 Saccharomyces Saccharomyces NNPS 10_1101-2021_01_07_425723 481 19 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 481 20 724 724 CD 10_1101-2021_01_07_425723 481 21 strains strain NNS 10_1101-2021_01_07_425723 481 22 for for IN 10_1101-2021_01_07_425723 481 23 the the DT 10_1101-2021_01_07_425723 481 24 bio bio NNP 10_1101-2021_01_07_425723 481 25 - - HYPH 10_1101-2021_01_07_425723 481 26 based base VBN 10_1101-2021_01_07_425723 481 27 economy economy NN 10_1101-2021_01_07_425723 481 28 from from IN 10_1101-2021_01_07_425723 481 29 biomass biomass NN 10_1101-2021_01_07_425723 481 30 : : : 10_1101-2021_01_07_425723 481 31 the the DT 10_1101-2021_01_07_425723 481 32 case case NN 10_1101-2021_01_07_425723 481 33 of of IN 10_1101-2021_01_07_425723 481 34 bioethanol bioethanol NN 10_1101-2021_01_07_425723 481 35 . . . 10_1101-2021_01_07_425723 482 1 Crit Crit NNP 10_1101-2021_01_07_425723 482 2 . . . 10_1101-2021_01_07_425723 483 1 Rev. Rev. NNP 10_1101-2021_01_07_425723 484 1 Biotechnol Biotechnol NNS 10_1101-2021_01_07_425723 484 2 . . . 10_1101-2021_01_07_425723 485 1 39 39 CD 10_1101-2021_01_07_425723 485 2 , , , 10_1101-2021_01_07_425723 485 3 725 725 CD 10_1101-2021_01_07_425723 485 4 800–816 800–816 CD 10_1101-2021_01_07_425723 485 5 ( ( -LRB- 10_1101-2021_01_07_425723 485 6 2019 2019 CD 10_1101-2021_01_07_425723 485 7 ) ) -RRB- 10_1101-2021_01_07_425723 485 8 . . . 10_1101-2021_01_07_425723 486 1 726 726 CD 10_1101-2021_01_07_425723 486 2 5 5 CD 10_1101-2021_01_07_425723 486 3 . . . 10_1101-2021_01_07_425723 487 1 Stovicek Stovicek NNP 10_1101-2021_01_07_425723 487 2 , , , 10_1101-2021_01_07_425723 487 3 V. V. NNP 10_1101-2021_01_07_425723 487 4 , , , 10_1101-2021_01_07_425723 487 5 Holkenbrink Holkenbrink NNP 10_1101-2021_01_07_425723 487 6 , , , 10_1101-2021_01_07_425723 487 7 C. C. NNP 10_1101-2021_01_07_425723 487 8 & & CC 10_1101-2021_01_07_425723 487 9 Borodina Borodina NNP 10_1101-2021_01_07_425723 487 10 , , , 10_1101-2021_01_07_425723 487 11 I. I. NNP 10_1101-2021_01_07_425723 487 12 CRISPR CRISPR NNP 10_1101-2021_01_07_425723 487 13 / / SYM 10_1101-2021_01_07_425723 487 14 Cas Cas NNP 10_1101-2021_01_07_425723 487 15 system system NN 10_1101-2021_01_07_425723 487 16 for for IN 10_1101-2021_01_07_425723 487 17 yeast yeast NN 10_1101-2021_01_07_425723 487 18 genome genome JJ 10_1101-2021_01_07_425723 487 19 engineering engineering NN 10_1101-2021_01_07_425723 487 20 : : : 10_1101-2021_01_07_425723 487 21 727 727 CD 10_1101-2021_01_07_425723 487 22 advances advance NNS 10_1101-2021_01_07_425723 487 23 and and CC 10_1101-2021_01_07_425723 487 24 applications application NNS 10_1101-2021_01_07_425723 487 25 . . . 10_1101-2021_01_07_425723 488 1 FEMS FEMS NNP 10_1101-2021_01_07_425723 488 2 Yeast Yeast NNP 10_1101-2021_01_07_425723 488 3 Res res NN 10_1101-2021_01_07_425723 488 4 . . . 10_1101-2021_01_07_425723 489 1 17 17 CD 10_1101-2021_01_07_425723 489 2 , , , 10_1101-2021_01_07_425723 489 3 1–16 1–16 CD 10_1101-2021_01_07_425723 489 4 ( ( -LRB- 10_1101-2021_01_07_425723 489 5 2017 2017 CD 10_1101-2021_01_07_425723 489 6 ) ) -RRB- 10_1101-2021_01_07_425723 489 7 . . . 10_1101-2021_01_07_425723 490 1 728 728 CD 10_1101-2021_01_07_425723 490 2 6 6 CD 10_1101-2021_01_07_425723 490 3 . . . 10_1101-2021_01_07_425723 491 1 Hong Hong NNP 10_1101-2021_01_07_425723 491 2 , , , 10_1101-2021_01_07_425723 491 3 J. J. NNP 10_1101-2021_01_07_425723 491 4 , , , 10_1101-2021_01_07_425723 491 5 Wang Wang NNP 10_1101-2021_01_07_425723 491 6 , , , 10_1101-2021_01_07_425723 491 7 Y. Y. NNP 10_1101-2021_01_07_425723 491 8 , , , 10_1101-2021_01_07_425723 491 9 Kumagai Kumagai NNP 10_1101-2021_01_07_425723 491 10 , , , 10_1101-2021_01_07_425723 491 11 H. H. NNP 10_1101-2021_01_07_425723 491 12 & & CC 10_1101-2021_01_07_425723 491 13 Tamaki Tamaki NNP 10_1101-2021_01_07_425723 491 14 , , , 10_1101-2021_01_07_425723 491 15 H. H. NNP 10_1101-2021_01_07_425723 491 16 Construction Construction NNP 10_1101-2021_01_07_425723 491 17 of of IN 10_1101-2021_01_07_425723 491 18 thermotolerant thermotolerant JJ 10_1101-2021_01_07_425723 491 19 yeast yeast NN 10_1101-2021_01_07_425723 491 20 expressing express VBG 10_1101-2021_01_07_425723 491 21 729 729 CD 10_1101-2021_01_07_425723 491 22 thermostable thermostable JJ 10_1101-2021_01_07_425723 491 23 cellulase cellulase NN 10_1101-2021_01_07_425723 491 24 genes gene NNS 10_1101-2021_01_07_425723 491 25 . . . 10_1101-2021_01_07_425723 492 1 J. J. NNP 10_1101-2021_01_07_425723 492 2 Biotechnol Biotechnol NNP 10_1101-2021_01_07_425723 492 3 . . . 10_1101-2021_01_07_425723 493 1 130 130 CD 10_1101-2021_01_07_425723 493 2 , , , 10_1101-2021_01_07_425723 493 3 114–123 114–123 CD 10_1101-2021_01_07_425723 493 4 ( ( -LRB- 10_1101-2021_01_07_425723 493 5 2007 2007 CD 10_1101-2021_01_07_425723 493 6 ) ) -RRB- 10_1101-2021_01_07_425723 493 7 . . . 10_1101-2021_01_07_425723 494 1 730 730 CD 10_1101-2021_01_07_425723 494 2 7 7 CD 10_1101-2021_01_07_425723 494 3 . . . 10_1101-2021_01_07_425723 495 1 Laman Laman NNP 10_1101-2021_01_07_425723 495 2 Trip Trip NNP 10_1101-2021_01_07_425723 495 3 , , , 10_1101-2021_01_07_425723 495 4 D. D. NNP 10_1101-2021_01_07_425723 495 5 S. S. NNP 10_1101-2021_01_07_425723 495 6 & & CC 10_1101-2021_01_07_425723 495 7 Youk Youk NNP 10_1101-2021_01_07_425723 495 8 , , , 10_1101-2021_01_07_425723 495 9 H. H. NNP 10_1101-2021_01_07_425723 495 10 Yeasts Yeasts NNPS 10_1101-2021_01_07_425723 495 11 collectively collectively RB 10_1101-2021_01_07_425723 495 12 extend extend VBP 10_1101-2021_01_07_425723 495 13 the the DT 10_1101-2021_01_07_425723 495 14 limits limit NNS 10_1101-2021_01_07_425723 495 15 of of IN 10_1101-2021_01_07_425723 495 16 habitable habitable JJ 10_1101-2021_01_07_425723 495 17 temperatures temperature NNS 10_1101-2021_01_07_425723 495 18 by by IN 10_1101-2021_01_07_425723 495 19 731 731 CD 10_1101-2021_01_07_425723 495 20 secreting secrete VBG 10_1101-2021_01_07_425723 495 21 glutathione glutathione NN 10_1101-2021_01_07_425723 495 22 . . . 10_1101-2021_01_07_425723 496 1 Nat Nat NNP 10_1101-2021_01_07_425723 496 2 . . . 10_1101-2021_01_07_425723 497 1 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 497 2 . . . 10_1101-2021_01_07_425723 498 1 5 5 CD 10_1101-2021_01_07_425723 498 2 , , , 10_1101-2021_01_07_425723 498 3 943–954 943–954 CD 10_1101-2021_01_07_425723 498 4 ( ( -LRB- 10_1101-2021_01_07_425723 498 5 2020 2020 CD 10_1101-2021_01_07_425723 498 6 ) ) -RRB- 10_1101-2021_01_07_425723 498 7 . . . 10_1101-2021_01_07_425723 499 1 732 732 CD 10_1101-2021_01_07_425723 499 2 8 8 CD 10_1101-2021_01_07_425723 499 3 . . . 10_1101-2021_01_07_425723 500 1 Choudhary Choudhary NNP 10_1101-2021_01_07_425723 500 2 , , , 10_1101-2021_01_07_425723 500 3 J. J. NNP 10_1101-2021_01_07_425723 500 4 , , , 10_1101-2021_01_07_425723 500 5 Singh Singh NNP 10_1101-2021_01_07_425723 500 6 , , , 10_1101-2021_01_07_425723 500 7 S. S. NNP 10_1101-2021_01_07_425723 500 8 & & CC 10_1101-2021_01_07_425723 500 9 Nain Nain NNP 10_1101-2021_01_07_425723 500 10 , , , 10_1101-2021_01_07_425723 500 11 L. L. NNP 10_1101-2021_01_07_425723 500 12 Thermotolerant Thermotolerant NNP 10_1101-2021_01_07_425723 500 13 fermenting ferment VBG 10_1101-2021_01_07_425723 500 14 yeasts yeast NNS 10_1101-2021_01_07_425723 500 15 for for IN 10_1101-2021_01_07_425723 500 16 simultaneous simultaneous JJ 10_1101-2021_01_07_425723 500 17 733 733 CD 10_1101-2021_01_07_425723 500 18 saccharification saccharification NN 10_1101-2021_01_07_425723 500 19 fermentation fermentation NN 10_1101-2021_01_07_425723 500 20 of of IN 10_1101-2021_01_07_425723 500 21 lignocellulosic lignocellulosic NN 10_1101-2021_01_07_425723 500 22 biomass biomass NN 10_1101-2021_01_07_425723 500 23 . . . 10_1101-2021_01_07_425723 501 1 Electron Electron NNP 10_1101-2021_01_07_425723 501 2 . . . 10_1101-2021_01_07_425723 502 1 J. J. NNP 10_1101-2021_01_07_425723 502 2 Biotechnol Biotechnol NNP 10_1101-2021_01_07_425723 502 3 . . . 10_1101-2021_01_07_425723 503 1 21 21 CD 10_1101-2021_01_07_425723 503 2 , , , 10_1101-2021_01_07_425723 503 3 82–92 82–92 CD 10_1101-2021_01_07_425723 503 4 ( ( -LRB- 10_1101-2021_01_07_425723 503 5 2016 2016 CD 10_1101-2021_01_07_425723 503 6 ) ) -RRB- 10_1101-2021_01_07_425723 503 7 . . . 10_1101-2021_01_07_425723 504 1 734 734 CD 10_1101-2021_01_07_425723 504 2 9 9 CD 10_1101-2021_01_07_425723 504 3 . . . 10_1101-2021_01_07_425723 505 1 Thorwall Thorwall NNP 10_1101-2021_01_07_425723 505 2 , , , 10_1101-2021_01_07_425723 505 3 S. S. NNP 10_1101-2021_01_07_425723 505 4 , , , 10_1101-2021_01_07_425723 505 5 Schwartz Schwartz NNP 10_1101-2021_01_07_425723 505 6 , , , 10_1101-2021_01_07_425723 505 7 C. C. NNP 10_1101-2021_01_07_425723 505 8 , , , 10_1101-2021_01_07_425723 505 9 Chartron Chartron NNP 10_1101-2021_01_07_425723 505 10 , , , 10_1101-2021_01_07_425723 505 11 J. J. NNP 10_1101-2021_01_07_425723 505 12 W. W. NNP 10_1101-2021_01_07_425723 505 13 & & CC 10_1101-2021_01_07_425723 505 14 Wheeldon Wheeldon NNP 10_1101-2021_01_07_425723 505 15 , , , 10_1101-2021_01_07_425723 505 16 I. I. NNP 10_1101-2021_01_07_425723 505 17 Stress stress NN 10_1101-2021_01_07_425723 505 18 - - HYPH 10_1101-2021_01_07_425723 505 19 tolerant tolerant JJ 10_1101-2021_01_07_425723 505 20 non non JJ 10_1101-2021_01_07_425723 505 21 - - JJ 10_1101-2021_01_07_425723 505 22 conventional conventional JJ 10_1101-2021_01_07_425723 505 23 735 735 CD 10_1101-2021_01_07_425723 505 24 microbes microbe NNS 10_1101-2021_01_07_425723 505 25 enable enable VBP 10_1101-2021_01_07_425723 505 26 next next JJ 10_1101-2021_01_07_425723 505 27 - - HYPH 10_1101-2021_01_07_425723 505 28 generation generation NN 10_1101-2021_01_07_425723 505 29 chemical chemical NN 10_1101-2021_01_07_425723 505 30 biosynthesis biosynthesis NN 10_1101-2021_01_07_425723 505 31 . . . 10_1101-2021_01_07_425723 506 1 Nat Nat NNP 10_1101-2021_01_07_425723 506 2 . . . 10_1101-2021_01_07_425723 507 1 Chem Chem NNP 10_1101-2021_01_07_425723 507 2 . . . 10_1101-2021_01_07_425723 508 1 Biol Biol NNP 10_1101-2021_01_07_425723 508 2 . . . 10_1101-2021_01_07_425723 509 1 16 16 CD 10_1101-2021_01_07_425723 509 2 , , , 10_1101-2021_01_07_425723 509 3 113–121 113–121 CD 10_1101-2021_01_07_425723 509 4 ( ( -LRB- 10_1101-2021_01_07_425723 509 5 2020 2020 CD 10_1101-2021_01_07_425723 509 6 ) ) -RRB- 10_1101-2021_01_07_425723 509 7 . . . 10_1101-2021_01_07_425723 510 1 736 736 CD 10_1101-2021_01_07_425723 510 2 10 10 CD 10_1101-2021_01_07_425723 510 3 . . . 10_1101-2021_01_07_425723 511 1 Mejía Mejía NNP 10_1101-2021_01_07_425723 511 2 - - HYPH 10_1101-2021_01_07_425723 511 3 Barajas Barajas NNP 10_1101-2021_01_07_425723 511 4 , , , 10_1101-2021_01_07_425723 511 5 J. J. NNP 10_1101-2021_01_07_425723 512 1 A. A. NNP 10_1101-2021_01_07_425723 512 2 et et FW 10_1101-2021_01_07_425723 512 3 al al NNP 10_1101-2021_01_07_425723 512 4 . . . 10_1101-2021_01_07_425723 513 1 Second second JJ 10_1101-2021_01_07_425723 513 2 - - HYPH 10_1101-2021_01_07_425723 513 3 Generation Generation NNP 10_1101-2021_01_07_425723 513 4 Bioethanol Bioethanol NNP 10_1101-2021_01_07_425723 513 5 Production Production NNP 10_1101-2021_01_07_425723 513 6 through through IN 10_1101-2021_01_07_425723 513 7 a a DT 10_1101-2021_01_07_425723 513 8 Simultaneous simultaneous JJ 10_1101-2021_01_07_425723 513 9 737 737 CD 10_1101-2021_01_07_425723 513 10 Saccharification Saccharification NNP 10_1101-2021_01_07_425723 513 11 - - HYPH 10_1101-2021_01_07_425723 513 12 Fermentation fermentation NN 10_1101-2021_01_07_425723 513 13 Process Process NNP 10_1101-2021_01_07_425723 513 14 Using Using NNP 10_1101-2021_01_07_425723 513 15 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 513 16 Marxianus Marxianus NNP 10_1101-2021_01_07_425723 513 17 Thermotolerant Thermotolerant NNP 10_1101-2021_01_07_425723 513 18 Yeast Yeast NNP 10_1101-2021_01_07_425723 513 19 . . . 10_1101-2021_01_07_425723 514 1 in in IN 10_1101-2021_01_07_425723 514 2 738 738 CD 10_1101-2021_01_07_425723 514 3 Special Special NNP 10_1101-2021_01_07_425723 514 4 Topics Topics NNPS 10_1101-2021_01_07_425723 514 5 in in IN 10_1101-2021_01_07_425723 514 6 Renewable renewable JJ 10_1101-2021_01_07_425723 514 7 Energy Energy NNP 10_1101-2021_01_07_425723 514 8 Systems Systems NNPS 10_1101-2021_01_07_425723 514 9 ( ( -LRB- 10_1101-2021_01_07_425723 514 10 InTech InTech NNP 10_1101-2021_01_07_425723 514 11 , , , 10_1101-2021_01_07_425723 514 12 2018 2018 CD 10_1101-2021_01_07_425723 514 13 ) ) -RRB- 10_1101-2021_01_07_425723 514 14 . . . 10_1101-2021_01_07_425723 515 1 doi:10.5772 doi:10.5772 NNP 10_1101-2021_01_07_425723 515 2 / / SYM 10_1101-2021_01_07_425723 515 3 intechopen.78052 intechopen.78052 NNP 10_1101-2021_01_07_425723 515 4 739 739 CD 10_1101-2021_01_07_425723 515 5 11 11 CD 10_1101-2021_01_07_425723 515 6 . . . 10_1101-2021_01_07_425723 516 1 Snoek Snoek NNP 10_1101-2021_01_07_425723 516 2 , , , 10_1101-2021_01_07_425723 516 3 I. I. NNP 10_1101-2021_01_07_425723 516 4 S. S. NNP 10_1101-2021_01_07_425723 516 5 I. I. NNP 10_1101-2021_01_07_425723 517 1 & & CC 10_1101-2021_01_07_425723 517 2 Steensma Steensma NNP 10_1101-2021_01_07_425723 517 3 , , , 10_1101-2021_01_07_425723 517 4 H. H. NNP 10_1101-2021_01_07_425723 517 5 Y. Y. NNP 10_1101-2021_01_07_425723 518 1 Why why WRB 10_1101-2021_01_07_425723 518 2 does do VBZ 10_1101-2021_01_07_425723 518 3 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 518 4 lactis lactis RB 10_1101-2021_01_07_425723 518 5 not not RB 10_1101-2021_01_07_425723 518 6 grow grow VB 10_1101-2021_01_07_425723 518 7 under under IN 10_1101-2021_01_07_425723 518 8 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 518 9 740 740 CD 10_1101-2021_01_07_425723 518 10 conditions condition NNS 10_1101-2021_01_07_425723 518 11 ? ? . 10_1101-2021_01_07_425723 519 1 Comparison comparison NN 10_1101-2021_01_07_425723 519 2 of of IN 10_1101-2021_01_07_425723 519 3 essential essential JJ 10_1101-2021_01_07_425723 519 4 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 519 5 genes gene NNS 10_1101-2021_01_07_425723 519 6 of of IN 10_1101-2021_01_07_425723 519 7 Saccharomyces Saccharomyces NNPS 10_1101-2021_01_07_425723 519 8 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 519 9 with with IN 10_1101-2021_01_07_425723 519 10 the the DT 10_1101-2021_01_07_425723 519 11 741 741 CD 10_1101-2021_01_07_425723 519 12 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 519 13 lactis lactis JJ 10_1101-2021_01_07_425723 519 14 genome genome NN 10_1101-2021_01_07_425723 519 15 . . . 10_1101-2021_01_07_425723 520 1 FEMS FEMS NNP 10_1101-2021_01_07_425723 520 2 Yeast Yeast NNP 10_1101-2021_01_07_425723 520 3 Res res NN 10_1101-2021_01_07_425723 520 4 . . . 10_1101-2021_01_07_425723 521 1 6 6 CD 10_1101-2021_01_07_425723 521 2 , , , 10_1101-2021_01_07_425723 521 3 393–403 393–403 CD 10_1101-2021_01_07_425723 521 4 ( ( -LRB- 10_1101-2021_01_07_425723 521 5 2006 2006 CD 10_1101-2021_01_07_425723 521 6 ) ) -RRB- 10_1101-2021_01_07_425723 521 7 . . . 10_1101-2021_01_07_425723 522 1 742 742 CD 10_1101-2021_01_07_425723 522 2 12 12 CD 10_1101-2021_01_07_425723 522 3 . . . 10_1101-2021_01_07_425723 523 1 Visser Visser NNP 10_1101-2021_01_07_425723 523 2 , , , 10_1101-2021_01_07_425723 523 3 W. W. NNP 10_1101-2021_01_07_425723 523 4 , , , 10_1101-2021_01_07_425723 523 5 Scheffers Scheffers NNP 10_1101-2021_01_07_425723 523 6 , , , 10_1101-2021_01_07_425723 523 7 W. W. NNP 10_1101-2021_01_07_425723 523 8 A. A. NNP 10_1101-2021_01_07_425723 523 9 , , , 10_1101-2021_01_07_425723 523 10 Batenburg Batenburg NNP 10_1101-2021_01_07_425723 523 11 - - HYPH 10_1101-2021_01_07_425723 523 12 Van Van NNP 10_1101-2021_01_07_425723 523 13 der der NN 10_1101-2021_01_07_425723 523 14 Vegte Vegte NNP 10_1101-2021_01_07_425723 523 15 , , , 10_1101-2021_01_07_425723 523 16 W. W. NNP 10_1101-2021_01_07_425723 523 17 H. H. NNP 10_1101-2021_01_07_425723 523 18 & & CC 10_1101-2021_01_07_425723 523 19 Van Van NNP 10_1101-2021_01_07_425723 523 20 Dijken Dijken NNP 10_1101-2021_01_07_425723 523 21 , , , 10_1101-2021_01_07_425723 523 22 J. J. NNP 10_1101-2021_01_07_425723 523 23 P. P. NNP 10_1101-2021_01_07_425723 523 24 Oxygen Oxygen NNP 10_1101-2021_01_07_425723 523 25 743 743 CD 10_1101-2021_01_07_425723 523 26 requirements requirement NNS 10_1101-2021_01_07_425723 523 27 of of IN 10_1101-2021_01_07_425723 523 28 yeasts yeast NNS 10_1101-2021_01_07_425723 523 29 . . . 10_1101-2021_01_07_425723 524 1 Appl Appl NNP 10_1101-2021_01_07_425723 524 2 . . . 10_1101-2021_01_07_425723 525 1 Environ Environ NNP 10_1101-2021_01_07_425723 525 2 . . . 10_1101-2021_01_07_425723 526 1 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 526 2 . . . 10_1101-2021_01_07_425723 527 1 56 56 CD 10_1101-2021_01_07_425723 527 2 , , , 10_1101-2021_01_07_425723 527 3 3785–3792 3785–3792 CD 10_1101-2021_01_07_425723 527 4 ( ( -LRB- 10_1101-2021_01_07_425723 527 5 1990 1990 CD 10_1101-2021_01_07_425723 527 6 ) ) -RRB- 10_1101-2021_01_07_425723 527 7 . . . 10_1101-2021_01_07_425723 528 1 744 744 CD 10_1101-2021_01_07_425723 528 2 13 13 CD 10_1101-2021_01_07_425723 528 3 . . . 10_1101-2021_01_07_425723 529 1 Merico Merico NNP 10_1101-2021_01_07_425723 529 2 , , , 10_1101-2021_01_07_425723 529 3 A. a. NN 10_1101-2021_01_07_425723 529 4 , , , 10_1101-2021_01_07_425723 529 5 Sulo Sulo NNP 10_1101-2021_01_07_425723 529 6 , , , 10_1101-2021_01_07_425723 529 7 P. P. NNP 10_1101-2021_01_07_425723 529 8 , , , 10_1101-2021_01_07_425723 529 9 Piškur Piškur NNP 10_1101-2021_01_07_425723 529 10 , , , 10_1101-2021_01_07_425723 529 11 J. J. NNP 10_1101-2021_01_07_425723 530 1 & & CC 10_1101-2021_01_07_425723 530 2 Compagno Compagno NNP 10_1101-2021_01_07_425723 530 3 , , , 10_1101-2021_01_07_425723 530 4 C. C. NNP 10_1101-2021_01_07_425723 530 5 Fermentative Fermentative NNP 10_1101-2021_01_07_425723 530 6 lifestyle lifestyle NN 10_1101-2021_01_07_425723 530 7 in in IN 10_1101-2021_01_07_425723 530 8 yeasts yeast NNS 10_1101-2021_01_07_425723 530 9 belonging belong VBG 10_1101-2021_01_07_425723 530 10 to to IN 10_1101-2021_01_07_425723 530 11 the the DT 10_1101-2021_01_07_425723 530 12 745 745 CD 10_1101-2021_01_07_425723 530 13 Saccharomyces Saccharomyces NNPS 10_1101-2021_01_07_425723 530 14 complex complex JJ 10_1101-2021_01_07_425723 530 15 . . . 10_1101-2021_01_07_425723 531 1 FEBS FEBS NNP 10_1101-2021_01_07_425723 531 2 J. J. NNP 10_1101-2021_01_07_425723 532 1 274 274 CD 10_1101-2021_01_07_425723 532 2 , , , 10_1101-2021_01_07_425723 532 3 976–989 976–989 CD 10_1101-2021_01_07_425723 532 4 ( ( -LRB- 10_1101-2021_01_07_425723 532 5 2007 2007 CD 10_1101-2021_01_07_425723 532 6 ) ) -RRB- 10_1101-2021_01_07_425723 532 7 . . . 10_1101-2021_01_07_425723 533 1 746 746 CD 10_1101-2021_01_07_425723 533 2 14 14 CD 10_1101-2021_01_07_425723 533 3 . . . 10_1101-2021_01_07_425723 534 1 Andreasen Andreasen NNP 10_1101-2021_01_07_425723 534 2 , , , 10_1101-2021_01_07_425723 534 3 A. a. NN 10_1101-2021_01_07_425723 535 1 A. A. NNP 10_1101-2021_01_07_425723 536 1 & & CC 10_1101-2021_01_07_425723 536 2 Stier Stier NNP 10_1101-2021_01_07_425723 536 3 , , , 10_1101-2021_01_07_425723 536 4 T. T. NNP 10_1101-2021_01_07_425723 536 5 J. J. NNP 10_1101-2021_01_07_425723 537 1 B. B. NNP 10_1101-2021_01_07_425723 537 2 Anaerobic anaerobic JJ 10_1101-2021_01_07_425723 537 3 nutrition nutrition NN 10_1101-2021_01_07_425723 537 4 of of IN 10_1101-2021_01_07_425723 537 5 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 537 6 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 537 7 I. I. NNP 10_1101-2021_01_07_425723 537 8 Ergosterol Ergosterol NNP 10_1101-2021_01_07_425723 537 9 747 747 CD 10_1101-2021_01_07_425723 537 10 requirement requirement NN 10_1101-2021_01_07_425723 537 11 for for IN 10_1101-2021_01_07_425723 537 12 growth growth NN 10_1101-2021_01_07_425723 537 13 in in IN 10_1101-2021_01_07_425723 537 14 a a DT 10_1101-2021_01_07_425723 537 15 defined define VBN 10_1101-2021_01_07_425723 537 16 medium medium NN 10_1101-2021_01_07_425723 537 17 . . . 10_1101-2021_01_07_425723 538 1 J. J. NNP 10_1101-2021_01_07_425723 539 1 Cell cell NN 10_1101-2021_01_07_425723 539 2 . . . 10_1101-2021_01_07_425723 540 1 Physiol Physiol NNP 10_1101-2021_01_07_425723 540 2 . . . 10_1101-2021_01_07_425723 541 1 41 41 CD 10_1101-2021_01_07_425723 541 2 , , , 10_1101-2021_01_07_425723 541 3 23–26 23–26 CD 10_1101-2021_01_07_425723 541 4 ( ( -LRB- 10_1101-2021_01_07_425723 541 5 1953 1953 CD 10_1101-2021_01_07_425723 541 6 ) ) -RRB- 10_1101-2021_01_07_425723 541 7 . . . 10_1101-2021_01_07_425723 542 1 748 748 CD 10_1101-2021_01_07_425723 542 2 15 15 CD 10_1101-2021_01_07_425723 542 3 . . . 10_1101-2021_01_07_425723 543 1 Andreasen Andreasen NNP 10_1101-2021_01_07_425723 543 2 , , , 10_1101-2021_01_07_425723 543 3 A. a. NN 10_1101-2021_01_07_425723 544 1 A. A. NNP 10_1101-2021_01_07_425723 545 1 & & CC 10_1101-2021_01_07_425723 545 2 Stier Stier NNP 10_1101-2021_01_07_425723 545 3 , , , 10_1101-2021_01_07_425723 545 4 T. T. NNP 10_1101-2021_01_07_425723 545 5 J. J. NNP 10_1101-2021_01_07_425723 546 1 B. B. NNP 10_1101-2021_01_07_425723 546 2 Anaerobic anaerobic JJ 10_1101-2021_01_07_425723 546 3 nutrition nutrition NN 10_1101-2021_01_07_425723 546 4 of of IN 10_1101-2021_01_07_425723 546 5 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 546 6 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 546 7 II ii CD 10_1101-2021_01_07_425723 546 8 . . . 10_1101-2021_01_07_425723 547 1 Unsaturated Unsaturated NNP 10_1101-2021_01_07_425723 547 2 749 749 CD 10_1101-2021_01_07_425723 547 3 fatty fatty NN 10_1101-2021_01_07_425723 547 4 acid acid NN 10_1101-2021_01_07_425723 547 5 requirement requirement NN 10_1101-2021_01_07_425723 547 6 for for IN 10_1101-2021_01_07_425723 547 7 growth growth NN 10_1101-2021_01_07_425723 547 8 in in IN 10_1101-2021_01_07_425723 547 9 a a DT 10_1101-2021_01_07_425723 547 10 defined define VBN 10_1101-2021_01_07_425723 547 11 medium medium NN 10_1101-2021_01_07_425723 547 12 . . . 10_1101-2021_01_07_425723 548 1 J. J. NNP 10_1101-2021_01_07_425723 549 1 Cell cell NN 10_1101-2021_01_07_425723 549 2 . . . 10_1101-2021_01_07_425723 550 1 Physiol Physiol NNP 10_1101-2021_01_07_425723 550 2 . . . 10_1101-2021_01_07_425723 551 1 43 43 CD 10_1101-2021_01_07_425723 551 2 , , , 10_1101-2021_01_07_425723 551 3 271–281 271–281 CD 10_1101-2021_01_07_425723 551 4 ( ( -LRB- 10_1101-2021_01_07_425723 551 5 1953 1953 CD 10_1101-2021_01_07_425723 551 6 ) ) -RRB- 10_1101-2021_01_07_425723 551 7 . . . 10_1101-2021_01_07_425723 552 1 750 750 CD 10_1101-2021_01_07_425723 552 2 16 16 CD 10_1101-2021_01_07_425723 552 3 . . . 10_1101-2021_01_07_425723 553 1 Passi Passi NNP 10_1101-2021_01_07_425723 553 2 , , , 10_1101-2021_01_07_425723 553 3 S. S. NNP 10_1101-2021_01_07_425723 553 4 et et NNP 10_1101-2021_01_07_425723 553 5 al al NNP 10_1101-2021_01_07_425723 553 6 . . . 10_1101-2021_01_07_425723 554 1 Saturated saturate VBN 10_1101-2021_01_07_425723 554 2 dicarboxylic dicarboxylic JJ 10_1101-2021_01_07_425723 554 3 acids acid NNS 10_1101-2021_01_07_425723 554 4 as as IN 10_1101-2021_01_07_425723 554 5 products product NNS 10_1101-2021_01_07_425723 554 6 of of IN 10_1101-2021_01_07_425723 554 7 unsaturated unsaturated JJ 10_1101-2021_01_07_425723 554 8 fatty fatty NN 10_1101-2021_01_07_425723 554 9 acid acid NN 10_1101-2021_01_07_425723 554 10 oxidation oxidation NN 10_1101-2021_01_07_425723 554 11 . . . 10_1101-2021_01_07_425723 555 1 751 751 CD 10_1101-2021_01_07_425723 555 2 Biochim Biochim NNP 10_1101-2021_01_07_425723 555 3 . . . 10_1101-2021_01_07_425723 556 1 Biophys Biophys NNP 10_1101-2021_01_07_425723 556 2 . . . 10_1101-2021_01_07_425723 557 1 Acta Acta NNP 10_1101-2021_01_07_425723 557 2 - - HYPH 10_1101-2021_01_07_425723 557 3 Lipids Lipids NNP 10_1101-2021_01_07_425723 557 4 Lipid Lipid NNP 10_1101-2021_01_07_425723 557 5 Metab Metab NNP 10_1101-2021_01_07_425723 557 6 . . . 10_1101-2021_01_07_425723 558 1 1168 1168 CD 10_1101-2021_01_07_425723 558 2 , , , 10_1101-2021_01_07_425723 558 3 190–198 190–198 CD 10_1101-2021_01_07_425723 558 4 ( ( -LRB- 10_1101-2021_01_07_425723 558 5 1993 1993 CD 10_1101-2021_01_07_425723 558 6 ) ) -RRB- 10_1101-2021_01_07_425723 558 7 . . . 10_1101-2021_01_07_425723 559 1 752 752 CD 10_1101-2021_01_07_425723 559 2 17 17 CD 10_1101-2021_01_07_425723 559 3 . . . 10_1101-2021_01_07_425723 560 1 Verduyn Verduyn NNP 10_1101-2021_01_07_425723 560 2 , , , 10_1101-2021_01_07_425723 560 3 C. C. NNP 10_1101-2021_01_07_425723 560 4 , , , 10_1101-2021_01_07_425723 560 5 Postma Postma NNP 10_1101-2021_01_07_425723 560 6 , , , 10_1101-2021_01_07_425723 560 7 E. E. NNP 10_1101-2021_01_07_425723 560 8 , , , 10_1101-2021_01_07_425723 560 9 Scheffers Scheffers NNP 10_1101-2021_01_07_425723 560 10 , , , 10_1101-2021_01_07_425723 560 11 W. W. NNP 10_1101-2021_01_07_425723 560 12 A. A. NNP 10_1101-2021_01_07_425723 561 1 & & CC 10_1101-2021_01_07_425723 561 2 van van NNP 10_1101-2021_01_07_425723 561 3 Dijken Dijken NNP 10_1101-2021_01_07_425723 561 4 , , , 10_1101-2021_01_07_425723 561 5 J. J. NNP 10_1101-2021_01_07_425723 561 6 P. P. NNP 10_1101-2021_01_07_425723 561 7 Physiology Physiology NNP 10_1101-2021_01_07_425723 561 8 of of IN 10_1101-2021_01_07_425723 561 9 Saccharomyces Saccharomyces NNP 10_1101-2021_01_07_425723 561 10 753 753 CD 10_1101-2021_01_07_425723 561 11 Cerevisiae Cerevisiae NNPS 10_1101-2021_01_07_425723 561 12 in in IN 10_1101-2021_01_07_425723 561 13 Anaerobic Anaerobic NNP 10_1101-2021_01_07_425723 561 14 Glucose Glucose NNP 10_1101-2021_01_07_425723 561 15 - - HYPH 10_1101-2021_01_07_425723 561 16 Limited Limited NNP 10_1101-2021_01_07_425723 561 17 Chemostat Chemostat NNP 10_1101-2021_01_07_425723 561 18 Cultures Cultures NNPS 10_1101-2021_01_07_425723 561 19 . . . 10_1101-2021_01_07_425723 562 1 J. J. NNP 10_1101-2021_01_07_425723 562 2 Gen. Gen. NNP 10_1101-2021_01_07_425723 562 3 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 562 4 . . . 10_1101-2021_01_07_425723 563 1 136 136 CD 10_1101-2021_01_07_425723 563 2 , , , 10_1101-2021_01_07_425723 563 3 395–403 395–403 CD 10_1101-2021_01_07_425723 563 4 754 754 CD 10_1101-2021_01_07_425723 563 5 .CC .CC : 10_1101-2021_01_07_425723 563 6 - - HYPH 10_1101-2021_01_07_425723 563 7 BY by IN 10_1101-2021_01_07_425723 563 8 - - HYPH 10_1101-2021_01_07_425723 563 9 NC NC NNP 10_1101-2021_01_07_425723 563 10 - - HYPH 10_1101-2021_01_07_425723 563 11 ND ND NNP 10_1101-2021_01_07_425723 563 12 4.0 4.0 CD 10_1101-2021_01_07_425723 563 13 International International NNP 10_1101-2021_01_07_425723 563 14 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 563 15 under under IN 10_1101-2021_01_07_425723 563 16 a a DT 10_1101-2021_01_07_425723 563 17 ( ( -LRB- 10_1101-2021_01_07_425723 563 18 which which WDT 10_1101-2021_01_07_425723 563 19 was be VBD 10_1101-2021_01_07_425723 563 20 not not RB 10_1101-2021_01_07_425723 563 21 certified certify VBN 10_1101-2021_01_07_425723 563 22 by by IN 10_1101-2021_01_07_425723 563 23 peer peer NN 10_1101-2021_01_07_425723 563 24 review review NN 10_1101-2021_01_07_425723 563 25 ) ) -RRB- 10_1101-2021_01_07_425723 563 26 is be VBZ 10_1101-2021_01_07_425723 563 27 the the DT 10_1101-2021_01_07_425723 563 28 author author NN 10_1101-2021_01_07_425723 563 29 / / SYM 10_1101-2021_01_07_425723 563 30 funder funder NN 10_1101-2021_01_07_425723 563 31 , , , 10_1101-2021_01_07_425723 563 32 who who WP 10_1101-2021_01_07_425723 563 33 has have VBZ 10_1101-2021_01_07_425723 563 34 granted grant VBN 10_1101-2021_01_07_425723 563 35 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 563 36 a a DT 10_1101-2021_01_07_425723 563 37 license license NN 10_1101-2021_01_07_425723 563 38 to to TO 10_1101-2021_01_07_425723 563 39 display display VB 10_1101-2021_01_07_425723 563 40 the the DT 10_1101-2021_01_07_425723 563 41 preprint preprint NN 10_1101-2021_01_07_425723 563 42 in in IN 10_1101-2021_01_07_425723 563 43 perpetuity perpetuity NN 10_1101-2021_01_07_425723 563 44 . . . 10_1101-2021_01_07_425723 564 1 It -PRON- PRP 10_1101-2021_01_07_425723 564 2 is be VBZ 10_1101-2021_01_07_425723 564 3 made make VBN 10_1101-2021_01_07_425723 564 4 The the DT 10_1101-2021_01_07_425723 564 5 copyright copyright NN 10_1101-2021_01_07_425723 564 6 holder holder NN 10_1101-2021_01_07_425723 564 7 for for IN 10_1101-2021_01_07_425723 564 8 this this DT 10_1101-2021_01_07_425723 564 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 564 10 version version NN 10_1101-2021_01_07_425723 564 11 posted post VBD 10_1101-2021_01_07_425723 564 12 January January NNP 10_1101-2021_01_07_425723 564 13 8 8 CD 10_1101-2021_01_07_425723 564 14 , , , 10_1101-2021_01_07_425723 564 15 2021 2021 CD 10_1101-2021_01_07_425723 564 16 . . . 10_1101-2021_01_07_425723 564 17 ; ; : 10_1101-2021_01_07_425723 564 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 564 19 : : : 10_1101-2021_01_07_425723 564 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 564 21 preprint preprint NN 10_1101-2021_01_07_425723 564 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 564 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 564 24 41 41 CD 10_1101-2021_01_07_425723 564 25 ( ( -LRB- 10_1101-2021_01_07_425723 564 26 1990 1990 CD 10_1101-2021_01_07_425723 564 27 ) ) -RRB- 10_1101-2021_01_07_425723 564 28 . . . 10_1101-2021_01_07_425723 565 1 755 755 CD 10_1101-2021_01_07_425723 565 2 18 18 CD 10_1101-2021_01_07_425723 565 3 . . . 10_1101-2021_01_07_425723 566 1 Perli Perli NNP 10_1101-2021_01_07_425723 566 2 , , , 10_1101-2021_01_07_425723 566 3 T. T. NNP 10_1101-2021_01_07_425723 566 4 , , , 10_1101-2021_01_07_425723 566 5 Wronska Wronska NNP 10_1101-2021_01_07_425723 566 6 , , , 10_1101-2021_01_07_425723 566 7 A. A. NNP 10_1101-2021_01_07_425723 566 8 K. K. NNP 10_1101-2021_01_07_425723 566 9 , , , 10_1101-2021_01_07_425723 566 10 Ortiz Ortiz NNP 10_1101-2021_01_07_425723 566 11 - - HYPH 10_1101-2021_01_07_425723 566 12 Merino Merino NNP 10_1101-2021_01_07_425723 566 13 , , , 10_1101-2021_01_07_425723 566 14 R. R. NNP 10_1101-2021_01_07_425723 566 15 A. A. NNP 10_1101-2021_01_07_425723 566 16 , , , 10_1101-2021_01_07_425723 566 17 Pronk Pronk NNP 10_1101-2021_01_07_425723 566 18 , , , 10_1101-2021_01_07_425723 566 19 J. J. NNP 10_1101-2021_01_07_425723 566 20 T. T. NNP 10_1101-2021_01_07_425723 566 21 & & CC 10_1101-2021_01_07_425723 566 22 Daran Daran NNP 10_1101-2021_01_07_425723 566 23 , , , 10_1101-2021_01_07_425723 566 24 J. J. NNP 10_1101-2021_01_07_425723 566 25 M. M. NNP 10_1101-2021_01_07_425723 566 26 Vitamin Vitamin NNP 10_1101-2021_01_07_425723 566 27 requirements requirement NNS 10_1101-2021_01_07_425723 566 28 and and CC 10_1101-2021_01_07_425723 566 29 756 756 CD 10_1101-2021_01_07_425723 566 30 biosynthesis biosynthesis NN 10_1101-2021_01_07_425723 566 31 in in IN 10_1101-2021_01_07_425723 566 32 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 566 33 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 566 34 . . . 10_1101-2021_01_07_425723 567 1 Yeast yeast NN 10_1101-2021_01_07_425723 567 2 1–22 1–22 CD 10_1101-2021_01_07_425723 567 3 ( ( -LRB- 10_1101-2021_01_07_425723 567 4 2020 2020 CD 10_1101-2021_01_07_425723 567 5 ) ) -RRB- 10_1101-2021_01_07_425723 567 6 . . . 10_1101-2021_01_07_425723 568 1 doi:10.1002 doi:10.1002 NNP 10_1101-2021_01_07_425723 568 2 / / SYM 10_1101-2021_01_07_425723 568 3 yea.3461 yea.3461 NNP 10_1101-2021_01_07_425723 568 4 757 757 CD 10_1101-2021_01_07_425723 568 5 19 19 CD 10_1101-2021_01_07_425723 568 6 . . . 10_1101-2021_01_07_425723 569 1 Dekker Dekker NNP 10_1101-2021_01_07_425723 569 2 , , , 10_1101-2021_01_07_425723 569 3 W. W. NNP 10_1101-2021_01_07_425723 569 4 J. J. NNP 10_1101-2021_01_07_425723 569 5 C. C. NNP 10_1101-2021_01_07_425723 569 6 , , , 10_1101-2021_01_07_425723 569 7 Wiersma Wiersma NNP 10_1101-2021_01_07_425723 569 8 , , , 10_1101-2021_01_07_425723 569 9 S. S. NNP 10_1101-2021_01_07_425723 569 10 J. J. NNP 10_1101-2021_01_07_425723 569 11 , , , 10_1101-2021_01_07_425723 569 12 Bouwknegt Bouwknegt NNP 10_1101-2021_01_07_425723 569 13 , , , 10_1101-2021_01_07_425723 569 14 J. J. NNP 10_1101-2021_01_07_425723 569 15 , , , 10_1101-2021_01_07_425723 569 16 Mooiman Mooiman NNP 10_1101-2021_01_07_425723 569 17 , , , 10_1101-2021_01_07_425723 569 18 C. C. NNP 10_1101-2021_01_07_425723 569 19 & & CC 10_1101-2021_01_07_425723 569 20 Pronk Pronk NNP 10_1101-2021_01_07_425723 569 21 , , , 10_1101-2021_01_07_425723 569 22 J. J. NNP 10_1101-2021_01_07_425723 569 23 T. T. NNP 10_1101-2021_01_07_425723 569 24 Anaerobic anaerobic JJ 10_1101-2021_01_07_425723 569 25 growth growth NN 10_1101-2021_01_07_425723 569 26 of of IN 10_1101-2021_01_07_425723 569 27 758 758 CD 10_1101-2021_01_07_425723 569 28 Saccharomyces Saccharomyces NNPS 10_1101-2021_01_07_425723 569 29 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 569 30 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 569 31 - - HYPH 10_1101-2021_01_07_425723 569 32 7D 7D NNP 10_1101-2021_01_07_425723 569 33 does do VBZ 10_1101-2021_01_07_425723 569 34 not not RB 10_1101-2021_01_07_425723 569 35 depend depend VB 10_1101-2021_01_07_425723 569 36 on on IN 10_1101-2021_01_07_425723 569 37 synthesis synthesis NN 10_1101-2021_01_07_425723 569 38 or or CC 10_1101-2021_01_07_425723 569 39 supplementation supplementation NN 10_1101-2021_01_07_425723 569 40 of of IN 10_1101-2021_01_07_425723 569 41 759 759 CD 10_1101-2021_01_07_425723 569 42 unsaturated unsaturated JJ 10_1101-2021_01_07_425723 569 43 fatty fatty NN 10_1101-2021_01_07_425723 569 44 acids acid NNS 10_1101-2021_01_07_425723 569 45 . . . 10_1101-2021_01_07_425723 570 1 FEMS FEMS NNP 10_1101-2021_01_07_425723 570 2 Yeast Yeast NNP 10_1101-2021_01_07_425723 570 3 Res res NN 10_1101-2021_01_07_425723 570 4 . . . 10_1101-2021_01_07_425723 571 1 19 19 CD 10_1101-2021_01_07_425723 571 2 , , , 10_1101-2021_01_07_425723 571 3 ( ( -LRB- 10_1101-2021_01_07_425723 571 4 2019 2019 CD 10_1101-2021_01_07_425723 571 5 ) ) -RRB- 10_1101-2021_01_07_425723 571 6 . . . 10_1101-2021_01_07_425723 572 1 760 760 CD 10_1101-2021_01_07_425723 572 2 20 20 CD 10_1101-2021_01_07_425723 572 3 . . . 10_1101-2021_01_07_425723 573 1 Wilcox Wilcox NNP 10_1101-2021_01_07_425723 573 2 , , , 10_1101-2021_01_07_425723 573 3 L. L. NNP 10_1101-2021_01_07_425723 573 4 J. J. NNP 10_1101-2021_01_07_425723 573 5 et et NNP 10_1101-2021_01_07_425723 573 6 al al NNP 10_1101-2021_01_07_425723 573 7 . . . 10_1101-2021_01_07_425723 574 1 Transcriptional transcriptional JJ 10_1101-2021_01_07_425723 574 2 profiling profiling NN 10_1101-2021_01_07_425723 574 3 identifies identifie NNS 10_1101-2021_01_07_425723 574 4 two two CD 10_1101-2021_01_07_425723 574 5 members member NNS 10_1101-2021_01_07_425723 574 6 of of IN 10_1101-2021_01_07_425723 574 7 the the DT 10_1101-2021_01_07_425723 574 8 ATP atp NN 10_1101-2021_01_07_425723 574 9 - - HYPH 10_1101-2021_01_07_425723 574 10 binding bind VBG 10_1101-2021_01_07_425723 574 11 cassette cassette NN 10_1101-2021_01_07_425723 574 12 761 761 CD 10_1101-2021_01_07_425723 574 13 transporter transporter NN 10_1101-2021_01_07_425723 574 14 superfamily superfamily RB 10_1101-2021_01_07_425723 574 15 required require VBN 10_1101-2021_01_07_425723 574 16 for for IN 10_1101-2021_01_07_425723 574 17 sterol sterol NN 10_1101-2021_01_07_425723 574 18 uptake uptake NNP 10_1101-2021_01_07_425723 574 19 in in IN 10_1101-2021_01_07_425723 574 20 yeast yeast NN 10_1101-2021_01_07_425723 574 21 . . . 10_1101-2021_01_07_425723 575 1 J. J. NNP 10_1101-2021_01_07_425723 575 2 Biol Biol NNP 10_1101-2021_01_07_425723 575 3 . . . 10_1101-2021_01_07_425723 576 1 Chem Chem NNP 10_1101-2021_01_07_425723 576 2 . . . 10_1101-2021_01_07_425723 577 1 277 277 CD 10_1101-2021_01_07_425723 577 2 , , , 10_1101-2021_01_07_425723 577 3 32466–32472 32466–32472 CD 10_1101-2021_01_07_425723 577 4 762 762 CD 10_1101-2021_01_07_425723 577 5 ( ( -LRB- 10_1101-2021_01_07_425723 577 6 2002 2002 CD 10_1101-2021_01_07_425723 577 7 ) ) -RRB- 10_1101-2021_01_07_425723 577 8 . . . 10_1101-2021_01_07_425723 578 1 763 763 CD 10_1101-2021_01_07_425723 578 2 21 21 CD 10_1101-2021_01_07_425723 578 3 . . . 10_1101-2021_01_07_425723 579 1 Black Black NNP 10_1101-2021_01_07_425723 579 2 , , , 10_1101-2021_01_07_425723 579 3 P. P. NNP 10_1101-2021_01_07_425723 579 4 N. N. NNP 10_1101-2021_01_07_425723 579 5 & & CC 10_1101-2021_01_07_425723 579 6 DiRusso DiRusso NNP 10_1101-2021_01_07_425723 579 7 , , , 10_1101-2021_01_07_425723 579 8 C. C. NNP 10_1101-2021_01_07_425723 579 9 C. C. NNP 10_1101-2021_01_07_425723 579 10 Yeast Yeast NNP 10_1101-2021_01_07_425723 579 11 acyl acyl NN 10_1101-2021_01_07_425723 579 12 - - HYPH 10_1101-2021_01_07_425723 579 13 CoA CoA NNP 10_1101-2021_01_07_425723 579 14 synthetases synthetase VBZ 10_1101-2021_01_07_425723 579 15 at at IN 10_1101-2021_01_07_425723 579 16 the the DT 10_1101-2021_01_07_425723 579 17 crossroads crossroad NNS 10_1101-2021_01_07_425723 579 18 of of IN 10_1101-2021_01_07_425723 579 19 fatty fatty NN 10_1101-2021_01_07_425723 579 20 acid acid VBP 10_1101-2021_01_07_425723 579 21 764 764 CD 10_1101-2021_01_07_425723 579 22 metabolism metabolism NN 10_1101-2021_01_07_425723 579 23 and and CC 10_1101-2021_01_07_425723 579 24 regulation regulation NN 10_1101-2021_01_07_425723 579 25 . . . 10_1101-2021_01_07_425723 580 1 Biochim Biochim NNP 10_1101-2021_01_07_425723 580 2 . . . 10_1101-2021_01_07_425723 581 1 Biophys Biophys NNP 10_1101-2021_01_07_425723 581 2 . . . 10_1101-2021_01_07_425723 582 1 Acta Acta NNP 10_1101-2021_01_07_425723 582 2 - - HYPH 10_1101-2021_01_07_425723 582 3 Mol Mol NNP 10_1101-2021_01_07_425723 582 4 . . . 10_1101-2021_01_07_425723 583 1 Cell Cell NNP 10_1101-2021_01_07_425723 583 2 Biol Biol NNP 10_1101-2021_01_07_425723 583 3 . . . 10_1101-2021_01_07_425723 584 1 Lipids lipid NNS 10_1101-2021_01_07_425723 584 2 1771 1771 CD 10_1101-2021_01_07_425723 584 3 , , , 10_1101-2021_01_07_425723 584 4 286–298 286–298 CD 10_1101-2021_01_07_425723 584 5 ( ( -LRB- 10_1101-2021_01_07_425723 584 6 2007 2007 CD 10_1101-2021_01_07_425723 584 7 ) ) -RRB- 10_1101-2021_01_07_425723 584 8 . . . 10_1101-2021_01_07_425723 585 1 765 765 CD 10_1101-2021_01_07_425723 585 2 22 22 CD 10_1101-2021_01_07_425723 585 3 . . . 10_1101-2021_01_07_425723 586 1 Jacquier Jacquier NNP 10_1101-2021_01_07_425723 586 2 , , , 10_1101-2021_01_07_425723 586 3 N. N. NNP 10_1101-2021_01_07_425723 586 4 & & CC 10_1101-2021_01_07_425723 586 5 Schneiter Schneiter NNP 10_1101-2021_01_07_425723 586 6 , , , 10_1101-2021_01_07_425723 586 7 R. R. NNP 10_1101-2021_01_07_425723 586 8 Ypk1 Ypk1 NNP 10_1101-2021_01_07_425723 586 9 , , , 10_1101-2021_01_07_425723 586 10 the the DT 10_1101-2021_01_07_425723 586 11 yeast yeast NN 10_1101-2021_01_07_425723 586 12 orthologue orthologue NN 10_1101-2021_01_07_425723 586 13 of of IN 10_1101-2021_01_07_425723 586 14 the the DT 10_1101-2021_01_07_425723 586 15 human human JJ 10_1101-2021_01_07_425723 586 16 serum- serum- NN 10_1101-2021_01_07_425723 586 17 and and CC 10_1101-2021_01_07_425723 586 18 glucocorticoid-766 glucocorticoid-766 NNP 10_1101-2021_01_07_425723 586 19 induced induce VBN 10_1101-2021_01_07_425723 586 20 kinase kinase NN 10_1101-2021_01_07_425723 586 21 , , , 10_1101-2021_01_07_425723 586 22 is be VBZ 10_1101-2021_01_07_425723 586 23 required require VBN 10_1101-2021_01_07_425723 586 24 for for IN 10_1101-2021_01_07_425723 586 25 efficient efficient JJ 10_1101-2021_01_07_425723 586 26 uptake uptake NN 10_1101-2021_01_07_425723 586 27 of of IN 10_1101-2021_01_07_425723 586 28 fatty fatty NN 10_1101-2021_01_07_425723 586 29 acids acid NNS 10_1101-2021_01_07_425723 586 30 . . . 10_1101-2021_01_07_425723 587 1 J. J. NNP 10_1101-2021_01_07_425723 588 1 Cell Cell NNP 10_1101-2021_01_07_425723 588 2 Sci Sci NNP 10_1101-2021_01_07_425723 588 3 . . . 10_1101-2021_01_07_425723 589 1 123 123 CD 10_1101-2021_01_07_425723 589 2 , , , 10_1101-2021_01_07_425723 589 3 2218–2227 2218–2227 CD 10_1101-2021_01_07_425723 589 4 ( ( -LRB- 10_1101-2021_01_07_425723 589 5 2010 2010 CD 10_1101-2021_01_07_425723 589 6 ) ) -RRB- 10_1101-2021_01_07_425723 589 7 . . . 10_1101-2021_01_07_425723 590 1 767 767 CD 10_1101-2021_01_07_425723 590 2 23 23 CD 10_1101-2021_01_07_425723 590 3 . . . 10_1101-2021_01_07_425723 591 1 Blomqvist Blomqvist NNP 10_1101-2021_01_07_425723 591 2 , , , 10_1101-2021_01_07_425723 591 3 J. J. NNP 10_1101-2021_01_07_425723 591 4 , , , 10_1101-2021_01_07_425723 591 5 Nogue Nogue NNP 10_1101-2021_01_07_425723 591 6 , , , 10_1101-2021_01_07_425723 591 7 V. V. NNP 10_1101-2021_01_07_425723 591 8 S. S. NNP 10_1101-2021_01_07_425723 591 9 , , , 10_1101-2021_01_07_425723 591 10 Gorwa Gorwa NNP 10_1101-2021_01_07_425723 591 11 - - HYPH 10_1101-2021_01_07_425723 591 12 Grauslund Grauslund NNP 10_1101-2021_01_07_425723 591 13 , , , 10_1101-2021_01_07_425723 591 14 M. M. NNP 10_1101-2021_01_07_425723 591 15 & & CC 10_1101-2021_01_07_425723 591 16 Passoth Passoth NNP 10_1101-2021_01_07_425723 591 17 , , , 10_1101-2021_01_07_425723 591 18 V. V. NNP 10_1101-2021_01_07_425723 591 19 Physiological physiological JJ 10_1101-2021_01_07_425723 591 20 requirements requirement NNS 10_1101-2021_01_07_425723 591 21 for for IN 10_1101-2021_01_07_425723 591 22 768 768 CD 10_1101-2021_01_07_425723 591 23 growth growth NN 10_1101-2021_01_07_425723 591 24 and and CC 10_1101-2021_01_07_425723 591 25 competitveness competitveness NN 10_1101-2021_01_07_425723 591 26 of of IN 10_1101-2021_01_07_425723 591 27 Dekkera Dekkera NNP 10_1101-2021_01_07_425723 591 28 bruxellensis bruxellensis NN 10_1101-2021_01_07_425723 591 29 under under IN 10_1101-2021_01_07_425723 591 30 oxygen oxygen NN 10_1101-2021_01_07_425723 591 31 limited limit VBN 10_1101-2021_01_07_425723 591 32 or or CC 10_1101-2021_01_07_425723 591 33 anaerobic anaerobic VBD 10_1101-2021_01_07_425723 591 34 769 769 CD 10_1101-2021_01_07_425723 591 35 conditions condition NNS 10_1101-2021_01_07_425723 591 36 . . . 10_1101-2021_01_07_425723 592 1 Yeast yeast NN 10_1101-2021_01_07_425723 592 2 29 29 CD 10_1101-2021_01_07_425723 592 3 , , , 10_1101-2021_01_07_425723 592 4 265–274 265–274 CD 10_1101-2021_01_07_425723 592 5 ( ( -LRB- 10_1101-2021_01_07_425723 592 6 2012 2012 CD 10_1101-2021_01_07_425723 592 7 ) ) -RRB- 10_1101-2021_01_07_425723 592 8 . . . 10_1101-2021_01_07_425723 593 1 770 770 CD 10_1101-2021_01_07_425723 593 2 24 24 CD 10_1101-2021_01_07_425723 593 3 . . . 10_1101-2021_01_07_425723 594 1 Zavrel Zavrel NNP 10_1101-2021_01_07_425723 594 2 , , , 10_1101-2021_01_07_425723 594 3 M. M. NNP 10_1101-2021_01_07_425723 594 4 , , , 10_1101-2021_01_07_425723 594 5 Hoot Hoot NNP 10_1101-2021_01_07_425723 594 6 , , , 10_1101-2021_01_07_425723 594 7 S. S. NNP 10_1101-2021_01_07_425723 594 8 J. J. NNP 10_1101-2021_01_07_425723 595 1 & & CC 10_1101-2021_01_07_425723 595 2 White White NNP 10_1101-2021_01_07_425723 595 3 , , , 10_1101-2021_01_07_425723 595 4 T. T. NNP 10_1101-2021_01_07_425723 595 5 C. C. NNP 10_1101-2021_01_07_425723 595 6 Comparison Comparison NNP 10_1101-2021_01_07_425723 595 7 of of IN 10_1101-2021_01_07_425723 595 8 sterol sterol NN 10_1101-2021_01_07_425723 595 9 import import NN 10_1101-2021_01_07_425723 595 10 under under IN 10_1101-2021_01_07_425723 595 11 aerobic aerobic JJ 10_1101-2021_01_07_425723 595 12 and and CC 10_1101-2021_01_07_425723 595 13 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 595 14 771 771 CD 10_1101-2021_01_07_425723 595 15 conditions condition NNS 10_1101-2021_01_07_425723 595 16 in in IN 10_1101-2021_01_07_425723 595 17 three three CD 10_1101-2021_01_07_425723 595 18 fungal fungal JJ 10_1101-2021_01_07_425723 595 19 species specie NNS 10_1101-2021_01_07_425723 595 20 , , , 10_1101-2021_01_07_425723 595 21 Candida Candida NNP 10_1101-2021_01_07_425723 595 22 albicans albican NNS 10_1101-2021_01_07_425723 595 23 , , , 10_1101-2021_01_07_425723 595 24 Candida Candida NNP 10_1101-2021_01_07_425723 595 25 glabrata glabrata NN 10_1101-2021_01_07_425723 595 26 , , , 10_1101-2021_01_07_425723 595 27 and and CC 10_1101-2021_01_07_425723 595 28 Saccharomyces saccharomyce VBZ 10_1101-2021_01_07_425723 595 29 772 772 CD 10_1101-2021_01_07_425723 595 30 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 595 31 . . . 10_1101-2021_01_07_425723 596 1 Eukaryot Eukaryot NNP 10_1101-2021_01_07_425723 596 2 . . . 10_1101-2021_01_07_425723 597 1 Cell cell NN 10_1101-2021_01_07_425723 597 2 12 12 CD 10_1101-2021_01_07_425723 597 3 , , , 10_1101-2021_01_07_425723 597 4 725–738 725–738 CD 10_1101-2021_01_07_425723 597 5 ( ( -LRB- 10_1101-2021_01_07_425723 597 6 2013 2013 CD 10_1101-2021_01_07_425723 597 7 ) ) -RRB- 10_1101-2021_01_07_425723 597 8 . . . 10_1101-2021_01_07_425723 598 1 773 773 CD 10_1101-2021_01_07_425723 598 2 25 25 CD 10_1101-2021_01_07_425723 598 3 . . . 10_1101-2021_01_07_425723 599 1 Visser Visser NNP 10_1101-2021_01_07_425723 599 2 , , , 10_1101-2021_01_07_425723 599 3 W. W. NNP 10_1101-2021_01_07_425723 599 4 , , , 10_1101-2021_01_07_425723 599 5 Scheffers Scheffers NNP 10_1101-2021_01_07_425723 599 6 , , , 10_1101-2021_01_07_425723 599 7 W. W. NNP 10_1101-2021_01_07_425723 599 8 A. A. NNP 10_1101-2021_01_07_425723 599 9 , , , 10_1101-2021_01_07_425723 599 10 Batenburg Batenburg NNP 10_1101-2021_01_07_425723 599 11 - - HYPH 10_1101-2021_01_07_425723 599 12 Van Van NNP 10_1101-2021_01_07_425723 599 13 der der NN 10_1101-2021_01_07_425723 599 14 Vegte Vegte NNP 10_1101-2021_01_07_425723 599 15 , , , 10_1101-2021_01_07_425723 599 16 W. W. NNP 10_1101-2021_01_07_425723 599 17 H. H. NNP 10_1101-2021_01_07_425723 599 18 & & CC 10_1101-2021_01_07_425723 599 19 Van Van NNP 10_1101-2021_01_07_425723 599 20 Dijken Dijken NNP 10_1101-2021_01_07_425723 599 21 , , , 10_1101-2021_01_07_425723 599 22 J. J. NNP 10_1101-2021_01_07_425723 599 23 P. P. NNP 10_1101-2021_01_07_425723 599 24 Oxygen Oxygen NNP 10_1101-2021_01_07_425723 599 25 774 774 CD 10_1101-2021_01_07_425723 599 26 requirements requirement NNS 10_1101-2021_01_07_425723 599 27 of of IN 10_1101-2021_01_07_425723 599 28 yeasts yeast NNS 10_1101-2021_01_07_425723 599 29 . . . 10_1101-2021_01_07_425723 600 1 Appl Appl NNP 10_1101-2021_01_07_425723 600 2 . . . 10_1101-2021_01_07_425723 601 1 Environ Environ NNP 10_1101-2021_01_07_425723 601 2 . . . 10_1101-2021_01_07_425723 602 1 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 602 2 . . . 10_1101-2021_01_07_425723 603 1 56 56 CD 10_1101-2021_01_07_425723 603 2 , , , 10_1101-2021_01_07_425723 603 3 3785–3792 3785–3792 CD 10_1101-2021_01_07_425723 603 4 ( ( -LRB- 10_1101-2021_01_07_425723 603 5 1990 1990 CD 10_1101-2021_01_07_425723 603 6 ) ) -RRB- 10_1101-2021_01_07_425723 603 7 . . . 10_1101-2021_01_07_425723 604 1 775 775 CD 10_1101-2021_01_07_425723 604 2 26 26 CD 10_1101-2021_01_07_425723 604 3 . . . 10_1101-2021_01_07_425723 605 1 Dashko Dashko NNP 10_1101-2021_01_07_425723 605 2 , , , 10_1101-2021_01_07_425723 605 3 S. S. NNP 10_1101-2021_01_07_425723 605 4 , , , 10_1101-2021_01_07_425723 605 5 Zhou Zhou NNP 10_1101-2021_01_07_425723 605 6 , , , 10_1101-2021_01_07_425723 605 7 N. N. NNP 10_1101-2021_01_07_425723 605 8 , , , 10_1101-2021_01_07_425723 605 9 Compagno Compagno NNP 10_1101-2021_01_07_425723 605 10 , , , 10_1101-2021_01_07_425723 605 11 C. C. NNP 10_1101-2021_01_07_425723 605 12 & & CC 10_1101-2021_01_07_425723 605 13 Piškur Piškur NNP 10_1101-2021_01_07_425723 605 14 , , , 10_1101-2021_01_07_425723 605 15 J. J. NNP 10_1101-2021_01_07_425723 606 1 Why why WRB 10_1101-2021_01_07_425723 606 2 , , , 10_1101-2021_01_07_425723 606 3 when when WRB 10_1101-2021_01_07_425723 606 4 , , , 10_1101-2021_01_07_425723 606 5 and and CC 10_1101-2021_01_07_425723 606 6 how how WRB 10_1101-2021_01_07_425723 606 7 did do VBD 10_1101-2021_01_07_425723 606 8 yeast yeast NN 10_1101-2021_01_07_425723 606 9 evolve evolve VB 10_1101-2021_01_07_425723 606 10 alcoholic alcoholic JJ 10_1101-2021_01_07_425723 606 11 776 776 CD 10_1101-2021_01_07_425723 606 12 fermentation fermentation NN 10_1101-2021_01_07_425723 606 13 ? ? . 10_1101-2021_01_07_425723 607 1 FEMS FEMS NNP 10_1101-2021_01_07_425723 607 2 Yeast Yeast NNP 10_1101-2021_01_07_425723 607 3 Res res NN 10_1101-2021_01_07_425723 607 4 . . . 10_1101-2021_01_07_425723 608 1 14 14 CD 10_1101-2021_01_07_425723 608 2 , , , 10_1101-2021_01_07_425723 608 3 826–832 826–832 CD 10_1101-2021_01_07_425723 608 4 ( ( -LRB- 10_1101-2021_01_07_425723 608 5 2014 2014 CD 10_1101-2021_01_07_425723 608 6 ) ) -RRB- 10_1101-2021_01_07_425723 608 7 . . . 10_1101-2021_01_07_425723 609 1 777 777 CD 10_1101-2021_01_07_425723 609 2 27 27 CD 10_1101-2021_01_07_425723 609 3 . . . 10_1101-2021_01_07_425723 610 1 Snoek Snoek NNP 10_1101-2021_01_07_425723 610 2 , , , 10_1101-2021_01_07_425723 610 3 I. I. NNP 10_1101-2021_01_07_425723 610 4 S. S. NNP 10_1101-2021_01_07_425723 610 5 I. I. NNP 10_1101-2021_01_07_425723 611 1 & & CC 10_1101-2021_01_07_425723 611 2 Steensma Steensma NNP 10_1101-2021_01_07_425723 611 3 , , , 10_1101-2021_01_07_425723 611 4 H. H. NNP 10_1101-2021_01_07_425723 611 5 Y. Y. NNP 10_1101-2021_01_07_425723 612 1 Factors factor NNS 10_1101-2021_01_07_425723 612 2 involved involve VBN 10_1101-2021_01_07_425723 612 3 in in IN 10_1101-2021_01_07_425723 612 4 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 612 5 growth growth NN 10_1101-2021_01_07_425723 612 6 of of IN 10_1101-2021_01_07_425723 612 7 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 612 8 778 778 CD 10_1101-2021_01_07_425723 612 9 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 612 10 . . . 10_1101-2021_01_07_425723 613 1 Yeast yeast NN 10_1101-2021_01_07_425723 613 2 24 24 CD 10_1101-2021_01_07_425723 613 3 , , , 10_1101-2021_01_07_425723 613 4 1–10 1–10 CD 10_1101-2021_01_07_425723 613 5 ( ( -LRB- 10_1101-2021_01_07_425723 613 6 2007 2007 CD 10_1101-2021_01_07_425723 613 7 ) ) -RRB- 10_1101-2021_01_07_425723 613 8 . . . 10_1101-2021_01_07_425723 614 1 779 779 CD 10_1101-2021_01_07_425723 614 2 28 28 CD 10_1101-2021_01_07_425723 614 3 . . . 10_1101-2021_01_07_425723 615 1 Vale Vale NNP 10_1101-2021_01_07_425723 615 2 da da NNP 10_1101-2021_01_07_425723 615 3 Costa Costa NNP 10_1101-2021_01_07_425723 615 4 , , , 10_1101-2021_01_07_425723 615 5 B. B. NNP 10_1101-2021_01_07_425723 615 6 L. L. NNP 10_1101-2021_01_07_425723 615 7 , , , 10_1101-2021_01_07_425723 615 8 Basso Basso NNP 10_1101-2021_01_07_425723 615 9 , , , 10_1101-2021_01_07_425723 615 10 T. T. NNP 10_1101-2021_01_07_425723 615 11 O. O. NNP 10_1101-2021_01_07_425723 615 12 , , , 10_1101-2021_01_07_425723 615 13 Raghavendran Raghavendran NNP 10_1101-2021_01_07_425723 615 14 , , , 10_1101-2021_01_07_425723 615 15 V. V. NNP 10_1101-2021_01_07_425723 615 16 & & CC 10_1101-2021_01_07_425723 615 17 Gombert Gombert NNP 10_1101-2021_01_07_425723 615 18 , , , 10_1101-2021_01_07_425723 615 19 A. A. NNP 10_1101-2021_01_07_425723 615 20 K. K. NNP 10_1101-2021_01_07_425723 615 21 Anaerobiosis Anaerobiosis NNP 10_1101-2021_01_07_425723 615 22 revisited revisit VBD 10_1101-2021_01_07_425723 615 23 : : : 10_1101-2021_01_07_425723 615 24 780 780 CD 10_1101-2021_01_07_425723 615 25 growth growth NN 10_1101-2021_01_07_425723 615 26 of of IN 10_1101-2021_01_07_425723 615 27 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 615 28 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 615 29 under under IN 10_1101-2021_01_07_425723 615 30 extremely extremely RB 10_1101-2021_01_07_425723 615 31 low low JJ 10_1101-2021_01_07_425723 615 32 oxygen oxygen NN 10_1101-2021_01_07_425723 615 33 availability availability NN 10_1101-2021_01_07_425723 615 34 . . . 10_1101-2021_01_07_425723 616 1 Appl Appl NNP 10_1101-2021_01_07_425723 616 2 . . . 10_1101-2021_01_07_425723 617 1 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 617 2 . . . 10_1101-2021_01_07_425723 618 1 781 781 CD 10_1101-2021_01_07_425723 618 2 Biotechnol Biotechnol NNPS 10_1101-2021_01_07_425723 618 3 . . . 10_1101-2021_01_07_425723 619 1 1–16 1–16 CD 10_1101-2021_01_07_425723 619 2 ( ( -LRB- 10_1101-2021_01_07_425723 619 3 2018 2018 CD 10_1101-2021_01_07_425723 619 4 ) ) -RRB- 10_1101-2021_01_07_425723 619 5 . . . 10_1101-2021_01_07_425723 620 1 doi:10.1007 doi:10.1007 NNP 10_1101-2021_01_07_425723 620 2 / / SYM 10_1101-2021_01_07_425723 620 3 s00253 s00253 NNP 10_1101-2021_01_07_425723 620 4 - - HYPH 10_1101-2021_01_07_425723 620 5 017 017 CD 10_1101-2021_01_07_425723 620 6 - - HYPH 10_1101-2021_01_07_425723 620 7 8732 8732 CD 10_1101-2021_01_07_425723 620 8 - - SYM 10_1101-2021_01_07_425723 620 9 4 4 CD 10_1101-2021_01_07_425723 620 10 782 782 CD 10_1101-2021_01_07_425723 620 11 29 29 CD 10_1101-2021_01_07_425723 620 12 . . . 10_1101-2021_01_07_425723 621 1 Wilkins Wilkins NNP 10_1101-2021_01_07_425723 621 2 , , , 10_1101-2021_01_07_425723 621 3 M. M. NNP 10_1101-2021_01_07_425723 621 4 R. R. NNP 10_1101-2021_01_07_425723 621 5 , , , 10_1101-2021_01_07_425723 621 6 Mueller Mueller NNP 10_1101-2021_01_07_425723 621 7 , , , 10_1101-2021_01_07_425723 621 8 M. M. NNP 10_1101-2021_01_07_425723 621 9 , , , 10_1101-2021_01_07_425723 621 10 Eichling Eichling NNP 10_1101-2021_01_07_425723 621 11 , , , 10_1101-2021_01_07_425723 621 12 S. S. NNP 10_1101-2021_01_07_425723 621 13 & & CC 10_1101-2021_01_07_425723 621 14 Banat Banat NNP 10_1101-2021_01_07_425723 621 15 , , , 10_1101-2021_01_07_425723 621 16 I. I. NNP 10_1101-2021_01_07_425723 621 17 M. M. NNP 10_1101-2021_01_07_425723 621 18 Fermentation Fermentation NNP 10_1101-2021_01_07_425723 621 19 of of IN 10_1101-2021_01_07_425723 621 20 xylose xylose NN 10_1101-2021_01_07_425723 621 21 by by IN 10_1101-2021_01_07_425723 621 22 the the DT 10_1101-2021_01_07_425723 621 23 783 783 CD 10_1101-2021_01_07_425723 621 24 thermotolerant thermotolerant JJ 10_1101-2021_01_07_425723 621 25 yeast yeast NN 10_1101-2021_01_07_425723 621 26 strains strain VBZ 10_1101-2021_01_07_425723 621 27 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 621 28 marxianus marxianus NN 10_1101-2021_01_07_425723 621 29 IMB2 IMB2 NNP 10_1101-2021_01_07_425723 621 30 , , , 10_1101-2021_01_07_425723 621 31 IMB4 IMB4 NNP 10_1101-2021_01_07_425723 621 32 , , , 10_1101-2021_01_07_425723 621 33 and and CC 10_1101-2021_01_07_425723 621 34 IMB5 IMB5 NNP 10_1101-2021_01_07_425723 621 35 under under IN 10_1101-2021_01_07_425723 621 36 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 621 37 784 784 CD 10_1101-2021_01_07_425723 621 38 conditions condition NNS 10_1101-2021_01_07_425723 621 39 . . . 10_1101-2021_01_07_425723 622 1 Process Process NNP 10_1101-2021_01_07_425723 622 2 Biochem Biochem NNP 10_1101-2021_01_07_425723 622 3 . . . 10_1101-2021_01_07_425723 623 1 43 43 CD 10_1101-2021_01_07_425723 623 2 , , , 10_1101-2021_01_07_425723 623 3 346–350 346–350 CD 10_1101-2021_01_07_425723 623 4 ( ( -LRB- 10_1101-2021_01_07_425723 623 5 2008 2008 CD 10_1101-2021_01_07_425723 623 6 ) ) -RRB- 10_1101-2021_01_07_425723 623 7 . . . 10_1101-2021_01_07_425723 624 1 785 785 CD 10_1101-2021_01_07_425723 624 2 30 30 CD 10_1101-2021_01_07_425723 624 3 . . . 10_1101-2021_01_07_425723 625 1 Hughes Hughes NNP 10_1101-2021_01_07_425723 625 2 , , , 10_1101-2021_01_07_425723 625 3 S. S. NNP 10_1101-2021_01_07_425723 625 4 R. R. NNP 10_1101-2021_01_07_425723 625 5 et et NNP 10_1101-2021_01_07_425723 625 6 al al NNP 10_1101-2021_01_07_425723 625 7 . . . 10_1101-2021_01_07_425723 626 1 Automated automated JJ 10_1101-2021_01_07_425723 626 2 UV uv NN 10_1101-2021_01_07_425723 626 3 - - HYPH 10_1101-2021_01_07_425723 626 4 C C NNP 10_1101-2021_01_07_425723 626 5 Mutagenesis Mutagenesis NNP 10_1101-2021_01_07_425723 626 6 of of IN 10_1101-2021_01_07_425723 626 7 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 626 8 marxianus marxianus NN 10_1101-2021_01_07_425723 626 9 NRRL NRRL NNP 10_1101-2021_01_07_425723 626 10 Y-1109 y-1109 NN 10_1101-2021_01_07_425723 626 11 and and CC 10_1101-2021_01_07_425723 626 12 786 786 CD 10_1101-2021_01_07_425723 626 13 Selection Selection NNP 10_1101-2021_01_07_425723 626 14 for for IN 10_1101-2021_01_07_425723 626 15 Microaerophilic Microaerophilic NNP 10_1101-2021_01_07_425723 626 16 Growth Growth NNP 10_1101-2021_01_07_425723 626 17 and and CC 10_1101-2021_01_07_425723 626 18 Ethanol Ethanol NNP 10_1101-2021_01_07_425723 626 19 Production Production NNP 10_1101-2021_01_07_425723 626 20 at at IN 10_1101-2021_01_07_425723 626 21 Elevated elevated JJ 10_1101-2021_01_07_425723 626 22 Temperature temperature NN 10_1101-2021_01_07_425723 626 23 on on IN 10_1101-2021_01_07_425723 626 24 787 787 CD 10_1101-2021_01_07_425723 626 25 Biomass Biomass NNP 10_1101-2021_01_07_425723 626 26 Sugars Sugars NNPS 10_1101-2021_01_07_425723 626 27 . . . 10_1101-2021_01_07_425723 627 1 J. J. NNP 10_1101-2021_01_07_425723 628 1 Lab lab NN 10_1101-2021_01_07_425723 628 2 . . . 10_1101-2021_01_07_425723 629 1 Autom autom NN 10_1101-2021_01_07_425723 629 2 . . . 10_1101-2021_01_07_425723 630 1 18 18 CD 10_1101-2021_01_07_425723 630 2 , , , 10_1101-2021_01_07_425723 630 3 276–290 276–290 CD 10_1101-2021_01_07_425723 630 4 ( ( -LRB- 10_1101-2021_01_07_425723 630 5 2013 2013 CD 10_1101-2021_01_07_425723 630 6 ) ) -RRB- 10_1101-2021_01_07_425723 630 7 . . . 10_1101-2021_01_07_425723 631 1 788 788 CD 10_1101-2021_01_07_425723 631 2 31 31 CD 10_1101-2021_01_07_425723 631 3 . . . 10_1101-2021_01_07_425723 632 1 Tetsuya Tetsuya NNP 10_1101-2021_01_07_425723 632 2 , , , 10_1101-2021_01_07_425723 632 3 G. G. NNP 10_1101-2021_01_07_425723 632 4 et et NNP 10_1101-2021_01_07_425723 632 5 al al NNP 10_1101-2021_01_07_425723 632 6 . . . 10_1101-2021_01_07_425723 633 1 Bioethanol Bioethanol NNP 10_1101-2021_01_07_425723 633 2 Production Production NNP 10_1101-2021_01_07_425723 633 3 from from IN 10_1101-2021_01_07_425723 633 4 Lignocellulosic Lignocellulosic NNP 10_1101-2021_01_07_425723 633 5 Biomass Biomass NNP 10_1101-2021_01_07_425723 633 6 by by IN 10_1101-2021_01_07_425723 633 7 a a DT 10_1101-2021_01_07_425723 633 8 Novel Novel NNP 10_1101-2021_01_07_425723 633 9 Kluyveromyces Kluyveromyces NNPS 10_1101-2021_01_07_425723 633 10 789 789 CD 10_1101-2021_01_07_425723 633 11 marxianus marxianus JJ 10_1101-2021_01_07_425723 633 12 Strain Strain NNP 10_1101-2021_01_07_425723 633 13 . . . 10_1101-2021_01_07_425723 634 1 Biosci Biosci NNP 10_1101-2021_01_07_425723 634 2 . . . 10_1101-2021_01_07_425723 635 1 Biotechnol Biotechnol NNS 10_1101-2021_01_07_425723 635 2 . . . 10_1101-2021_01_07_425723 636 1 Biochem Biochem NNP 10_1101-2021_01_07_425723 636 2 . . . 10_1101-2021_01_07_425723 637 1 77 77 CD 10_1101-2021_01_07_425723 637 2 , , , 10_1101-2021_01_07_425723 637 3 1505–1510 1505–1510 CD 10_1101-2021_01_07_425723 637 4 ( ( -LRB- 10_1101-2021_01_07_425723 637 5 2013 2013 CD 10_1101-2021_01_07_425723 637 6 ) ) -RRB- 10_1101-2021_01_07_425723 637 7 . . . 10_1101-2021_01_07_425723 638 1 790 790 CD 10_1101-2021_01_07_425723 638 2 32 32 CD 10_1101-2021_01_07_425723 638 3 . . . 10_1101-2021_01_07_425723 638 4 van van NNP 10_1101-2021_01_07_425723 638 5 Urk Urk NNP 10_1101-2021_01_07_425723 638 6 , , , 10_1101-2021_01_07_425723 638 7 H. H. NNP 10_1101-2021_01_07_425723 638 8 , , , 10_1101-2021_01_07_425723 638 9 Postma Postma NNP 10_1101-2021_01_07_425723 638 10 , , , 10_1101-2021_01_07_425723 638 11 E. E. NNP 10_1101-2021_01_07_425723 638 12 , , , 10_1101-2021_01_07_425723 638 13 Scheffers Scheffers NNP 10_1101-2021_01_07_425723 638 14 , , , 10_1101-2021_01_07_425723 638 15 W. W. NNP 10_1101-2021_01_07_425723 638 16 A. A. NNP 10_1101-2021_01_07_425723 639 1 & & CC 10_1101-2021_01_07_425723 639 2 van van NNP 10_1101-2021_01_07_425723 639 3 Dijken Dijken NNP 10_1101-2021_01_07_425723 639 4 , , , 10_1101-2021_01_07_425723 639 5 J. J. NNP 10_1101-2021_01_07_425723 639 6 P. P. NNP 10_1101-2021_01_07_425723 639 7 Glucose Glucose NNP 10_1101-2021_01_07_425723 639 8 Transport Transport NNP 10_1101-2021_01_07_425723 639 9 in in IN 10_1101-2021_01_07_425723 639 10 Crabtree Crabtree NNP 10_1101-2021_01_07_425723 639 11 - - HYPH 10_1101-2021_01_07_425723 639 12 positive positive JJ 10_1101-2021_01_07_425723 639 13 791 791 CD 10_1101-2021_01_07_425723 639 14 and and CC 10_1101-2021_01_07_425723 639 15 Crabtree Crabtree NNP 10_1101-2021_01_07_425723 639 16 - - HYPH 10_1101-2021_01_07_425723 639 17 negative negative JJ 10_1101-2021_01_07_425723 639 18 Yeasts Yeasts NNPS 10_1101-2021_01_07_425723 639 19 . . . 10_1101-2021_01_07_425723 640 1 J. J. NNP 10_1101-2021_01_07_425723 640 2 Gen. Gen. NNP 10_1101-2021_01_07_425723 640 3 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 640 4 . . . 10_1101-2021_01_07_425723 641 1 135 135 CD 10_1101-2021_01_07_425723 641 2 , , , 10_1101-2021_01_07_425723 641 3 2399–2406 2399–2406 CD 10_1101-2021_01_07_425723 641 4 ( ( -LRB- 10_1101-2021_01_07_425723 641 5 1989 1989 CD 10_1101-2021_01_07_425723 641 6 ) ) -RRB- 10_1101-2021_01_07_425723 641 7 . . . 10_1101-2021_01_07_425723 642 1 792 792 CD 10_1101-2021_01_07_425723 642 2 33 33 CD 10_1101-2021_01_07_425723 642 3 . . . 10_1101-2021_01_07_425723 642 4 von von NNP 10_1101-2021_01_07_425723 642 5 Meyenburg Meyenburg NNP 10_1101-2021_01_07_425723 642 6 , , , 10_1101-2021_01_07_425723 642 7 K. K. NNP 10_1101-2021_01_07_425723 642 8 Katabolit Katabolit NNP 10_1101-2021_01_07_425723 642 9 - - HYPH 10_1101-2021_01_07_425723 642 10 Repression Repression NNP 10_1101-2021_01_07_425723 642 11 und und RB 10_1101-2021_01_07_425723 642 12 der der IN 10_1101-2021_01_07_425723 642 13 Sprossungszyklus Sprossungszyklus NNP 10_1101-2021_01_07_425723 642 14 von von NNP 10_1101-2021_01_07_425723 642 15 Saccharomyces Saccharomyces NNP 10_1101-2021_01_07_425723 642 16 793 793 CD 10_1101-2021_01_07_425723 642 17 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 642 18 . . . 10_1101-2021_01_07_425723 643 1 ( ( -LRB- 10_1101-2021_01_07_425723 643 2 ETH ETH NNP 10_1101-2021_01_07_425723 643 3 Zürich Zürich NNP 10_1101-2021_01_07_425723 643 4 , , , 10_1101-2021_01_07_425723 643 5 1969 1969 CD 10_1101-2021_01_07_425723 643 6 ) ) -RRB- 10_1101-2021_01_07_425723 643 7 . . . 10_1101-2021_01_07_425723 644 1 doi:10.3929 doi:10.3929 LS 10_1101-2021_01_07_425723 644 2 / / SYM 10_1101-2021_01_07_425723 644 3 ethz ethz NNP 10_1101-2021_01_07_425723 644 4 - - HYPH 10_1101-2021_01_07_425723 644 5 a-000099923 a-000099923 CD 10_1101-2021_01_07_425723 644 6 794 794 CD 10_1101-2021_01_07_425723 644 7 .CC .CC : 10_1101-2021_01_07_425723 644 8 - - HYPH 10_1101-2021_01_07_425723 644 9 BY by IN 10_1101-2021_01_07_425723 644 10 - - HYPH 10_1101-2021_01_07_425723 644 11 NC NC NNP 10_1101-2021_01_07_425723 644 12 - - HYPH 10_1101-2021_01_07_425723 644 13 ND ND NNP 10_1101-2021_01_07_425723 644 14 4.0 4.0 CD 10_1101-2021_01_07_425723 644 15 International International NNP 10_1101-2021_01_07_425723 644 16 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 644 17 under under IN 10_1101-2021_01_07_425723 644 18 a a DT 10_1101-2021_01_07_425723 644 19 ( ( -LRB- 10_1101-2021_01_07_425723 644 20 which which WDT 10_1101-2021_01_07_425723 644 21 was be VBD 10_1101-2021_01_07_425723 644 22 not not RB 10_1101-2021_01_07_425723 644 23 certified certify VBN 10_1101-2021_01_07_425723 644 24 by by IN 10_1101-2021_01_07_425723 644 25 peer peer NN 10_1101-2021_01_07_425723 644 26 review review NN 10_1101-2021_01_07_425723 644 27 ) ) -RRB- 10_1101-2021_01_07_425723 644 28 is be VBZ 10_1101-2021_01_07_425723 644 29 the the DT 10_1101-2021_01_07_425723 644 30 author author NN 10_1101-2021_01_07_425723 644 31 / / SYM 10_1101-2021_01_07_425723 644 32 funder funder NN 10_1101-2021_01_07_425723 644 33 , , , 10_1101-2021_01_07_425723 644 34 who who WP 10_1101-2021_01_07_425723 644 35 has have VBZ 10_1101-2021_01_07_425723 644 36 granted grant VBN 10_1101-2021_01_07_425723 644 37 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 644 38 a a DT 10_1101-2021_01_07_425723 644 39 license license NN 10_1101-2021_01_07_425723 644 40 to to TO 10_1101-2021_01_07_425723 644 41 display display VB 10_1101-2021_01_07_425723 644 42 the the DT 10_1101-2021_01_07_425723 644 43 preprint preprint NN 10_1101-2021_01_07_425723 644 44 in in IN 10_1101-2021_01_07_425723 644 45 perpetuity perpetuity NN 10_1101-2021_01_07_425723 644 46 . . . 10_1101-2021_01_07_425723 645 1 It -PRON- PRP 10_1101-2021_01_07_425723 645 2 is be VBZ 10_1101-2021_01_07_425723 645 3 made make VBN 10_1101-2021_01_07_425723 645 4 The the DT 10_1101-2021_01_07_425723 645 5 copyright copyright NN 10_1101-2021_01_07_425723 645 6 holder holder NN 10_1101-2021_01_07_425723 645 7 for for IN 10_1101-2021_01_07_425723 645 8 this this DT 10_1101-2021_01_07_425723 645 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 645 10 version version NN 10_1101-2021_01_07_425723 645 11 posted post VBD 10_1101-2021_01_07_425723 645 12 January January NNP 10_1101-2021_01_07_425723 645 13 8 8 CD 10_1101-2021_01_07_425723 645 14 , , , 10_1101-2021_01_07_425723 645 15 2021 2021 CD 10_1101-2021_01_07_425723 645 16 . . . 10_1101-2021_01_07_425723 645 17 ; ; : 10_1101-2021_01_07_425723 645 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 645 19 : : : 10_1101-2021_01_07_425723 645 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 645 21 preprint preprint NN 10_1101-2021_01_07_425723 645 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 645 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 645 24 42 42 CD 10_1101-2021_01_07_425723 645 25 34 34 CD 10_1101-2021_01_07_425723 645 26 . . . 10_1101-2021_01_07_425723 646 1 Rouwenhorst Rouwenhorst NNP 10_1101-2021_01_07_425723 646 2 , , , 10_1101-2021_01_07_425723 646 3 R. R. NNP 10_1101-2021_01_07_425723 646 4 J. J. NNP 10_1101-2021_01_07_425723 646 5 , , , 10_1101-2021_01_07_425723 646 6 Visser Visser NNP 10_1101-2021_01_07_425723 646 7 , , , 10_1101-2021_01_07_425723 646 8 L. L. NNP 10_1101-2021_01_07_425723 646 9 E. E. NNP 10_1101-2021_01_07_425723 646 10 , , , 10_1101-2021_01_07_425723 646 11 Van Van NNP 10_1101-2021_01_07_425723 646 12 Der Der NNP 10_1101-2021_01_07_425723 646 13 Baan Baan NNP 10_1101-2021_01_07_425723 646 14 , , , 10_1101-2021_01_07_425723 646 15 A. A. NNP 10_1101-2021_01_07_425723 647 1 A. A. NNP 10_1101-2021_01_07_425723 647 2 , , , 10_1101-2021_01_07_425723 647 3 Scheffers Scheffers NNP 10_1101-2021_01_07_425723 647 4 , , , 10_1101-2021_01_07_425723 647 5 W. W. NNP 10_1101-2021_01_07_425723 647 6 A. A. NNP 10_1101-2021_01_07_425723 648 1 & & CC 10_1101-2021_01_07_425723 648 2 Van Van NNP 10_1101-2021_01_07_425723 648 3 Dijken Dijken NNP 10_1101-2021_01_07_425723 648 4 , , , 10_1101-2021_01_07_425723 648 5 J. J. NNP 10_1101-2021_01_07_425723 648 6 P. P. NNP 10_1101-2021_01_07_425723 648 7 795 795 CD 10_1101-2021_01_07_425723 648 8 Production Production NNP 10_1101-2021_01_07_425723 648 9 , , , 10_1101-2021_01_07_425723 648 10 Distribution distribution NN 10_1101-2021_01_07_425723 648 11 , , , 10_1101-2021_01_07_425723 648 12 and and CC 10_1101-2021_01_07_425723 648 13 Kinetic Kinetic NNP 10_1101-2021_01_07_425723 648 14 Properties Properties NNPS 10_1101-2021_01_07_425723 648 15 of of IN 10_1101-2021_01_07_425723 648 16 Inulinase Inulinase NNP 10_1101-2021_01_07_425723 648 17 in in IN 10_1101-2021_01_07_425723 648 18 Continuous continuous JJ 10_1101-2021_01_07_425723 648 19 Cultures Cultures NNPS 10_1101-2021_01_07_425723 648 20 of of IN 10_1101-2021_01_07_425723 648 21 796 796 CD 10_1101-2021_01_07_425723 648 22 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 648 23 marxianus marxianus NN 10_1101-2021_01_07_425723 648 24 CBS CBS NNP 10_1101-2021_01_07_425723 648 25 6556 6556 CD 10_1101-2021_01_07_425723 648 26 . . . 10_1101-2021_01_07_425723 649 1 Appl Appl NNP 10_1101-2021_01_07_425723 649 2 . . . 10_1101-2021_01_07_425723 650 1 Environ Environ NNP 10_1101-2021_01_07_425723 650 2 . . . 10_1101-2021_01_07_425723 651 1 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 651 2 . . . 10_1101-2021_01_07_425723 652 1 54 54 CD 10_1101-2021_01_07_425723 652 2 , , , 10_1101-2021_01_07_425723 652 3 1131–1137 1131–1137 CD 10_1101-2021_01_07_425723 652 4 ( ( -LRB- 10_1101-2021_01_07_425723 652 5 1988 1988 CD 10_1101-2021_01_07_425723 652 6 ) ) -RRB- 10_1101-2021_01_07_425723 652 7 . . . 10_1101-2021_01_07_425723 653 1 797 797 CD 10_1101-2021_01_07_425723 653 2 35 35 CD 10_1101-2021_01_07_425723 653 3 . . . 10_1101-2021_01_07_425723 654 1 Bakker Bakker NNP 10_1101-2021_01_07_425723 654 2 , , , 10_1101-2021_01_07_425723 654 3 B. B. NNP 10_1101-2021_01_07_425723 654 4 M. M. NNP 10_1101-2021_01_07_425723 654 5 et et NNP 10_1101-2021_01_07_425723 654 6 al al NNP 10_1101-2021_01_07_425723 654 7 . . . 10_1101-2021_01_07_425723 655 1 Stoichiometry stoichiometry NN 10_1101-2021_01_07_425723 655 2 and and CC 10_1101-2021_01_07_425723 655 3 compartmentation compartmentation NN 10_1101-2021_01_07_425723 655 4 of of IN 10_1101-2021_01_07_425723 655 5 NADH NADH NNP 10_1101-2021_01_07_425723 655 6 metabolism metabolism NN 10_1101-2021_01_07_425723 655 7 in in IN 10_1101-2021_01_07_425723 655 8 Saccharomyces Saccharomyces NNP 10_1101-2021_01_07_425723 655 9 798 798 CD 10_1101-2021_01_07_425723 655 10 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 655 11 . . . 10_1101-2021_01_07_425723 656 1 FEMS FEMS NNP 10_1101-2021_01_07_425723 656 2 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 656 3 . . . 10_1101-2021_01_07_425723 657 1 Rev. Rev. NNP 10_1101-2021_01_07_425723 658 1 25 25 CD 10_1101-2021_01_07_425723 658 2 , , , 10_1101-2021_01_07_425723 658 3 15–37 15–37 CD 10_1101-2021_01_07_425723 658 4 ( ( -LRB- 10_1101-2021_01_07_425723 658 5 2001 2001 CD 10_1101-2021_01_07_425723 658 6 ) ) -RRB- 10_1101-2021_01_07_425723 658 7 . . . 10_1101-2021_01_07_425723 659 1 799 799 CD 10_1101-2021_01_07_425723 659 2 36 36 CD 10_1101-2021_01_07_425723 659 3 . . . 10_1101-2021_01_07_425723 660 1 Jeong Jeong NNP 10_1101-2021_01_07_425723 660 2 , , , 10_1101-2021_01_07_425723 660 3 H. H. NNP 10_1101-2021_01_07_425723 660 4 et et NNP 10_1101-2021_01_07_425723 660 5 al al NNP 10_1101-2021_01_07_425723 660 6 . . . 10_1101-2021_01_07_425723 661 1 Genome genome JJ 10_1101-2021_01_07_425723 661 2 sequence sequence NN 10_1101-2021_01_07_425723 661 3 of of IN 10_1101-2021_01_07_425723 661 4 the the DT 10_1101-2021_01_07_425723 661 5 thermotolerant thermotolerant JJ 10_1101-2021_01_07_425723 661 6 yeast yeast NN 10_1101-2021_01_07_425723 661 7 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 661 8 marxianus marxianus JJ 10_1101-2021_01_07_425723 661 9 var var NN 10_1101-2021_01_07_425723 661 10 . . . 10_1101-2021_01_07_425723 662 1 800 800 CD 10_1101-2021_01_07_425723 662 2 marxianus marxianus JJ 10_1101-2021_01_07_425723 662 3 KCTC KCTC NNP 10_1101-2021_01_07_425723 662 4 17555 17555 CD 10_1101-2021_01_07_425723 662 5 . . . 10_1101-2021_01_07_425723 663 1 Eukaryot Eukaryot NNP 10_1101-2021_01_07_425723 663 2 . . . 10_1101-2021_01_07_425723 664 1 Cell cell NN 10_1101-2021_01_07_425723 664 2 11 11 CD 10_1101-2021_01_07_425723 664 3 , , , 10_1101-2021_01_07_425723 664 4 1584–1585 1584–1585 CD 10_1101-2021_01_07_425723 664 5 ( ( -LRB- 10_1101-2021_01_07_425723 664 6 2012 2012 CD 10_1101-2021_01_07_425723 664 7 ) ) -RRB- 10_1101-2021_01_07_425723 664 8 . . . 10_1101-2021_01_07_425723 665 1 801 801 CD 10_1101-2021_01_07_425723 665 2 37 37 CD 10_1101-2021_01_07_425723 665 3 . . . 10_1101-2021_01_07_425723 666 1 Jordá Jordá NNP 10_1101-2021_01_07_425723 666 2 , , , 10_1101-2021_01_07_425723 666 3 T. T. NNP 10_1101-2021_01_07_425723 666 4 & & CC 10_1101-2021_01_07_425723 666 5 Puig Puig NNP 10_1101-2021_01_07_425723 666 6 , , , 10_1101-2021_01_07_425723 666 7 S. S. NNP 10_1101-2021_01_07_425723 666 8 Regulation Regulation NNP 10_1101-2021_01_07_425723 666 9 of of IN 10_1101-2021_01_07_425723 666 10 Ergosterol Ergosterol NNP 10_1101-2021_01_07_425723 666 11 Biosynthesis Biosynthesis NNP 10_1101-2021_01_07_425723 666 12 in in IN 10_1101-2021_01_07_425723 666 13 Saccharomyces Saccharomyces NNP 10_1101-2021_01_07_425723 666 14 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 666 15 . . . 10_1101-2021_01_07_425723 667 1 Genes gene NNS 10_1101-2021_01_07_425723 667 2 802 802 CD 10_1101-2021_01_07_425723 667 3 ( ( -LRB- 10_1101-2021_01_07_425723 667 4 Basel Basel NNP 10_1101-2021_01_07_425723 667 5 ) ) -RRB- 10_1101-2021_01_07_425723 667 6 . . . 10_1101-2021_01_07_425723 668 1 11 11 CD 10_1101-2021_01_07_425723 668 2 , , , 10_1101-2021_01_07_425723 668 3 795 795 CD 10_1101-2021_01_07_425723 668 4 ( ( -LRB- 10_1101-2021_01_07_425723 668 5 2020 2020 CD 10_1101-2021_01_07_425723 668 6 ) ) -RRB- 10_1101-2021_01_07_425723 668 7 . . . 10_1101-2021_01_07_425723 669 1 803 803 CD 10_1101-2021_01_07_425723 669 2 38 38 CD 10_1101-2021_01_07_425723 669 3 . . . 10_1101-2021_01_07_425723 670 1 Lechner Lechner NNP 10_1101-2021_01_07_425723 670 2 , , , 10_1101-2021_01_07_425723 670 3 M. M. NNP 10_1101-2021_01_07_425723 670 4 et et NNP 10_1101-2021_01_07_425723 670 5 al al NNP 10_1101-2021_01_07_425723 670 6 . . . 10_1101-2021_01_07_425723 671 1 Proteinortho Proteinortho NNP 10_1101-2021_01_07_425723 671 2 : : : 10_1101-2021_01_07_425723 671 3 Detection detection NN 10_1101-2021_01_07_425723 671 4 of of IN 10_1101-2021_01_07_425723 671 5 ( ( -LRB- 10_1101-2021_01_07_425723 671 6 Co-)orthologs Co-)orthologs NNP 10_1101-2021_01_07_425723 671 7 in in IN 10_1101-2021_01_07_425723 671 8 large large JJ 10_1101-2021_01_07_425723 671 9 - - HYPH 10_1101-2021_01_07_425723 671 10 scale scale NN 10_1101-2021_01_07_425723 671 11 analysis analysis NN 10_1101-2021_01_07_425723 671 12 . . . 10_1101-2021_01_07_425723 672 1 BMC BMC NNP 10_1101-2021_01_07_425723 672 2 804 804 CD 10_1101-2021_01_07_425723 672 3 Bioinformatics Bioinformatics NNP 10_1101-2021_01_07_425723 672 4 12 12 CD 10_1101-2021_01_07_425723 672 5 , , , 10_1101-2021_01_07_425723 672 6 124 124 CD 10_1101-2021_01_07_425723 672 7 ( ( -LRB- 10_1101-2021_01_07_425723 672 8 2011 2011 CD 10_1101-2021_01_07_425723 672 9 ) ) -RRB- 10_1101-2021_01_07_425723 672 10 . . . 10_1101-2021_01_07_425723 673 1 805 805 CD 10_1101-2021_01_07_425723 673 2 39 39 CD 10_1101-2021_01_07_425723 673 3 . . . 10_1101-2021_01_07_425723 674 1 Nagy Nagy NNP 10_1101-2021_01_07_425723 674 2 , , , 10_1101-2021_01_07_425723 674 3 M. M. NNP 10_1101-2021_01_07_425723 674 4 , , , 10_1101-2021_01_07_425723 674 5 Lacroute Lacroute NNP 10_1101-2021_01_07_425723 674 6 , , , 10_1101-2021_01_07_425723 674 7 F. F. NNP 10_1101-2021_01_07_425723 674 8 & & CC 10_1101-2021_01_07_425723 674 9 Thomas Thomas NNP 10_1101-2021_01_07_425723 674 10 , , , 10_1101-2021_01_07_425723 674 11 D. D. NNP 10_1101-2021_01_07_425723 674 12 Divergent Divergent NNP 10_1101-2021_01_07_425723 674 13 evolution evolution NN 10_1101-2021_01_07_425723 674 14 of of IN 10_1101-2021_01_07_425723 674 15 pyrimidine pyrimidine JJ 10_1101-2021_01_07_425723 674 16 biosynthesis biosynthesis NN 10_1101-2021_01_07_425723 674 17 between between IN 10_1101-2021_01_07_425723 674 18 806 806 CD 10_1101-2021_01_07_425723 674 19 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 674 20 and and CC 10_1101-2021_01_07_425723 674 21 aerobic aerobic JJ 10_1101-2021_01_07_425723 674 22 yeasts yeast NNS 10_1101-2021_01_07_425723 674 23 . . . 10_1101-2021_01_07_425723 675 1 Proc Proc NNP 10_1101-2021_01_07_425723 675 2 . . . 10_1101-2021_01_07_425723 676 1 Natl Natl NNP 10_1101-2021_01_07_425723 676 2 . . . 10_1101-2021_01_07_425723 677 1 Acad Acad NNS 10_1101-2021_01_07_425723 677 2 . . . 10_1101-2021_01_07_425723 678 1 Sci Sci NNP 10_1101-2021_01_07_425723 678 2 . . . 10_1101-2021_01_07_425723 679 1 U. U. NNP 10_1101-2021_01_07_425723 679 2 S. S. NNP 10_1101-2021_01_07_425723 679 3 A. a. NN 10_1101-2021_01_07_425723 680 1 89 89 CD 10_1101-2021_01_07_425723 680 2 , , , 10_1101-2021_01_07_425723 680 3 8966–8970 8966–8970 CD 10_1101-2021_01_07_425723 680 4 ( ( -LRB- 10_1101-2021_01_07_425723 680 5 1992 1992 CD 10_1101-2021_01_07_425723 680 6 ) ) -RRB- 10_1101-2021_01_07_425723 680 7 . . . 10_1101-2021_01_07_425723 681 1 807 807 CD 10_1101-2021_01_07_425723 681 2 40 40 CD 10_1101-2021_01_07_425723 681 3 . . . 10_1101-2021_01_07_425723 682 1 Väremo Väremo NNP 10_1101-2021_01_07_425723 682 2 , , , 10_1101-2021_01_07_425723 682 3 L. L. NNP 10_1101-2021_01_07_425723 682 4 , , , 10_1101-2021_01_07_425723 682 5 Nielsen Nielsen NNP 10_1101-2021_01_07_425723 682 6 , , , 10_1101-2021_01_07_425723 682 7 J. J. NNP 10_1101-2021_01_07_425723 683 1 & & CC 10_1101-2021_01_07_425723 683 2 Nookaew Nookaew NNP 10_1101-2021_01_07_425723 683 3 , , , 10_1101-2021_01_07_425723 683 4 I. I. NNP 10_1101-2021_01_07_425723 684 1 Enriching enrich VBG 10_1101-2021_01_07_425723 684 2 the the DT 10_1101-2021_01_07_425723 684 3 gene gene NN 10_1101-2021_01_07_425723 684 4 set set VBD 10_1101-2021_01_07_425723 684 5 analysis analysis NN 10_1101-2021_01_07_425723 684 6 of of IN 10_1101-2021_01_07_425723 684 7 genome genome NN 10_1101-2021_01_07_425723 684 8 - - HYPH 10_1101-2021_01_07_425723 684 9 wide wide JJ 10_1101-2021_01_07_425723 684 10 data datum NNS 10_1101-2021_01_07_425723 684 11 by by IN 10_1101-2021_01_07_425723 684 12 808 808 CD 10_1101-2021_01_07_425723 684 13 incorporating incorporate VBG 10_1101-2021_01_07_425723 684 14 directionality directionality NN 10_1101-2021_01_07_425723 684 15 of of IN 10_1101-2021_01_07_425723 684 16 gene gene NN 10_1101-2021_01_07_425723 684 17 expression expression NN 10_1101-2021_01_07_425723 684 18 and and CC 10_1101-2021_01_07_425723 684 19 combining combine VBG 10_1101-2021_01_07_425723 684 20 statistical statistical JJ 10_1101-2021_01_07_425723 684 21 hypotheses hypothesis NNS 10_1101-2021_01_07_425723 684 22 and and CC 10_1101-2021_01_07_425723 684 23 809 809 CD 10_1101-2021_01_07_425723 684 24 methods method NNS 10_1101-2021_01_07_425723 684 25 . . . 10_1101-2021_01_07_425723 685 1 Nucleic Nucleic NNP 10_1101-2021_01_07_425723 685 2 Acids Acids NNPS 10_1101-2021_01_07_425723 685 3 Res Res NNP 10_1101-2021_01_07_425723 685 4 . . . 10_1101-2021_01_07_425723 686 1 41 41 CD 10_1101-2021_01_07_425723 686 2 , , , 10_1101-2021_01_07_425723 686 3 4378–4391 4378–4391 CD 10_1101-2021_01_07_425723 686 4 ( ( -LRB- 10_1101-2021_01_07_425723 686 5 2013 2013 CD 10_1101-2021_01_07_425723 686 6 ) ) -RRB- 10_1101-2021_01_07_425723 686 7 . . . 10_1101-2021_01_07_425723 687 1 810 810 CD 10_1101-2021_01_07_425723 687 2 41 41 CD 10_1101-2021_01_07_425723 687 3 . . . 10_1101-2021_01_07_425723 688 1 Tai Tai NNP 10_1101-2021_01_07_425723 688 2 , , , 10_1101-2021_01_07_425723 688 3 S. S. NNP 10_1101-2021_01_07_425723 688 4 L. L. NNP 10_1101-2021_01_07_425723 688 5 et et NNP 10_1101-2021_01_07_425723 688 6 al al NNP 10_1101-2021_01_07_425723 688 7 . . . 10_1101-2021_01_07_425723 689 1 Two two CD 10_1101-2021_01_07_425723 689 2 - - HYPH 10_1101-2021_01_07_425723 689 3 dimensional dimensional JJ 10_1101-2021_01_07_425723 689 4 transcriptome transcriptome NNP 10_1101-2021_01_07_425723 689 5 analysis analysis NN 10_1101-2021_01_07_425723 689 6 in in IN 10_1101-2021_01_07_425723 689 7 chemostat chemostat JJ 10_1101-2021_01_07_425723 689 8 cultures culture NNS 10_1101-2021_01_07_425723 689 9 : : : 10_1101-2021_01_07_425723 689 10 Combinatorial combinatorial NN 10_1101-2021_01_07_425723 689 11 811 811 CD 10_1101-2021_01_07_425723 689 12 effects effect NNS 10_1101-2021_01_07_425723 689 13 of of IN 10_1101-2021_01_07_425723 689 14 oxygen oxygen NN 10_1101-2021_01_07_425723 689 15 availability availability NN 10_1101-2021_01_07_425723 689 16 and and CC 10_1101-2021_01_07_425723 689 17 macronutrient macronutrient NN 10_1101-2021_01_07_425723 689 18 limitation limitation NN 10_1101-2021_01_07_425723 689 19 in in IN 10_1101-2021_01_07_425723 689 20 Saccharomyces Saccharomyces NNP 10_1101-2021_01_07_425723 689 21 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 689 22 . . . 10_1101-2021_01_07_425723 690 1 J. J. NNP 10_1101-2021_01_07_425723 690 2 Biol Biol NNP 10_1101-2021_01_07_425723 690 3 . . . 10_1101-2021_01_07_425723 691 1 812 812 CD 10_1101-2021_01_07_425723 691 2 Chem Chem NNP 10_1101-2021_01_07_425723 691 3 . . . 10_1101-2021_01_07_425723 692 1 280 280 CD 10_1101-2021_01_07_425723 692 2 , , , 10_1101-2021_01_07_425723 692 3 437–447 437–447 CD 10_1101-2021_01_07_425723 692 4 ( ( -LRB- 10_1101-2021_01_07_425723 692 5 2005 2005 CD 10_1101-2021_01_07_425723 692 6 ) ) -RRB- 10_1101-2021_01_07_425723 692 7 . . . 10_1101-2021_01_07_425723 693 1 813 813 CD 10_1101-2021_01_07_425723 693 2 42 42 CD 10_1101-2021_01_07_425723 693 3 . . . 10_1101-2021_01_07_425723 694 1 Alimardani Alimardani NNP 10_1101-2021_01_07_425723 694 2 , , , 10_1101-2021_01_07_425723 694 3 P. P. NNP 10_1101-2021_01_07_425723 694 4 et et NNP 10_1101-2021_01_07_425723 694 5 al al NNP 10_1101-2021_01_07_425723 694 6 . . . 10_1101-2021_01_07_425723 695 1 SUT1-promoted sut1-promote VBN 10_1101-2021_01_07_425723 695 2 sterol sterol NN 10_1101-2021_01_07_425723 695 3 uptake uptake NN 10_1101-2021_01_07_425723 695 4 involves involve VBZ 10_1101-2021_01_07_425723 695 5 the the DT 10_1101-2021_01_07_425723 695 6 ABC ABC NNP 10_1101-2021_01_07_425723 695 7 transporter transporter NN 10_1101-2021_01_07_425723 695 8 Aus1 Aus1 NNP 10_1101-2021_01_07_425723 695 9 and and CC 10_1101-2021_01_07_425723 695 10 the the DT 10_1101-2021_01_07_425723 695 11 814 814 CD 10_1101-2021_01_07_425723 695 12 mannoprotein mannoprotein NN 10_1101-2021_01_07_425723 695 13 Dan1 Dan1 NNP 10_1101-2021_01_07_425723 695 14 whose whose WP$ 10_1101-2021_01_07_425723 695 15 synergistic synergistic JJ 10_1101-2021_01_07_425723 695 16 action action NN 10_1101-2021_01_07_425723 695 17 is be VBZ 10_1101-2021_01_07_425723 695 18 sufficient sufficient JJ 10_1101-2021_01_07_425723 695 19 for for IN 10_1101-2021_01_07_425723 695 20 this this DT 10_1101-2021_01_07_425723 695 21 process process NN 10_1101-2021_01_07_425723 695 22 . . . 10_1101-2021_01_07_425723 696 1 Biochem Biochem NNP 10_1101-2021_01_07_425723 696 2 . . . 10_1101-2021_01_07_425723 697 1 J. J. NNP 10_1101-2021_01_07_425723 698 1 381 381 CD 10_1101-2021_01_07_425723 698 2 , , , 10_1101-2021_01_07_425723 698 3 195–815 195–815 CD 10_1101-2021_01_07_425723 698 4 202 202 CD 10_1101-2021_01_07_425723 698 5 ( ( -LRB- 10_1101-2021_01_07_425723 698 6 2004 2004 CD 10_1101-2021_01_07_425723 698 7 ) ) -RRB- 10_1101-2021_01_07_425723 698 8 . . . 10_1101-2021_01_07_425723 699 1 816 816 CD 10_1101-2021_01_07_425723 699 2 43 43 CD 10_1101-2021_01_07_425723 699 3 . . . 10_1101-2021_01_07_425723 700 1 Marek Marek NNP 10_1101-2021_01_07_425723 700 2 , , , 10_1101-2021_01_07_425723 700 3 M. M. NNP 10_1101-2021_01_07_425723 700 4 , , , 10_1101-2021_01_07_425723 700 5 Silvestro Silvestro NNP 10_1101-2021_01_07_425723 700 6 , , , 10_1101-2021_01_07_425723 700 7 D. D. NNP 10_1101-2021_01_07_425723 700 8 , , , 10_1101-2021_01_07_425723 700 9 Fredslund Fredslund NNP 10_1101-2021_01_07_425723 700 10 , , , 10_1101-2021_01_07_425723 700 11 M. M. NNP 10_1101-2021_01_07_425723 700 12 D. D. NNP 10_1101-2021_01_07_425723 700 13 , , , 10_1101-2021_01_07_425723 700 14 Andersen Andersen NNP 10_1101-2021_01_07_425723 700 15 , , , 10_1101-2021_01_07_425723 700 16 T. T. NNP 10_1101-2021_01_07_425723 700 17 G. G. NNP 10_1101-2021_01_07_425723 700 18 & & CC 10_1101-2021_01_07_425723 700 19 Pomorski Pomorski NNP 10_1101-2021_01_07_425723 700 20 , , , 10_1101-2021_01_07_425723 700 21 T. T. NNP 10_1101-2021_01_07_425723 700 22 G. G. NNP 10_1101-2021_01_07_425723 700 23 Serum Serum NNP 10_1101-2021_01_07_425723 700 24 albumin albumin NN 10_1101-2021_01_07_425723 700 25 817 817 CD 10_1101-2021_01_07_425723 700 26 promotes promote VBZ 10_1101-2021_01_07_425723 700 27 ATP atp NN 10_1101-2021_01_07_425723 700 28 - - HYPH 10_1101-2021_01_07_425723 700 29 binding bind VBG 10_1101-2021_01_07_425723 700 30 cassette cassette NN 10_1101-2021_01_07_425723 700 31 transporter transporter NN 10_1101-2021_01_07_425723 700 32 - - HYPH 10_1101-2021_01_07_425723 700 33 dependent dependent JJ 10_1101-2021_01_07_425723 700 34 sterol sterol NN 10_1101-2021_01_07_425723 700 35 uptake uptake NN 10_1101-2021_01_07_425723 700 36 in in IN 10_1101-2021_01_07_425723 700 37 yeast yeast NN 10_1101-2021_01_07_425723 700 38 . . . 10_1101-2021_01_07_425723 701 1 FEMS FEMS NNP 10_1101-2021_01_07_425723 701 2 Yeast Yeast NNP 10_1101-2021_01_07_425723 701 3 Res res NN 10_1101-2021_01_07_425723 701 4 . . . 10_1101-2021_01_07_425723 702 1 818 818 CD 10_1101-2021_01_07_425723 702 2 14 14 CD 10_1101-2021_01_07_425723 702 3 , , , 10_1101-2021_01_07_425723 702 4 1223–1233 1223–1233 CD 10_1101-2021_01_07_425723 702 5 ( ( -LRB- 10_1101-2021_01_07_425723 702 6 2014 2014 CD 10_1101-2021_01_07_425723 702 7 ) ) -RRB- 10_1101-2021_01_07_425723 702 8 . . . 10_1101-2021_01_07_425723 703 1 819 819 CD 10_1101-2021_01_07_425723 703 2 44 44 CD 10_1101-2021_01_07_425723 703 3 . . . 10_1101-2021_01_07_425723 704 1 Marek Marek NNP 10_1101-2021_01_07_425723 704 2 , , , 10_1101-2021_01_07_425723 704 3 M. M. NNP 10_1101-2021_01_07_425723 704 4 et et NNP 10_1101-2021_01_07_425723 704 5 al al NNP 10_1101-2021_01_07_425723 704 6 . . . 10_1101-2021_01_07_425723 705 1 The the DT 10_1101-2021_01_07_425723 705 2 yeast yeast NN 10_1101-2021_01_07_425723 705 3 plasma plasma NN 10_1101-2021_01_07_425723 705 4 membrane membrane NN 10_1101-2021_01_07_425723 705 5 ATP ATP NNP 10_1101-2021_01_07_425723 705 6 binding bind VBG 10_1101-2021_01_07_425723 705 7 cassette cassette NN 10_1101-2021_01_07_425723 705 8 ( ( -LRB- 10_1101-2021_01_07_425723 705 9 ABC ABC NNP 10_1101-2021_01_07_425723 705 10 ) ) -RRB- 10_1101-2021_01_07_425723 705 11 transporter transporter NN 10_1101-2021_01_07_425723 705 12 Aus1 Aus1 NNP 10_1101-2021_01_07_425723 705 13 : : : 10_1101-2021_01_07_425723 705 14 820 820 CD 10_1101-2021_01_07_425723 705 15 Purification Purification NNP 10_1101-2021_01_07_425723 705 16 , , , 10_1101-2021_01_07_425723 705 17 characterization characterization NN 10_1101-2021_01_07_425723 705 18 , , , 10_1101-2021_01_07_425723 705 19 and and CC 10_1101-2021_01_07_425723 705 20 the the DT 10_1101-2021_01_07_425723 705 21 effect effect NN 10_1101-2021_01_07_425723 705 22 of of IN 10_1101-2021_01_07_425723 705 23 lipids lipid NNS 10_1101-2021_01_07_425723 705 24 on on IN 10_1101-2021_01_07_425723 705 25 its -PRON- PRP$ 10_1101-2021_01_07_425723 705 26 activity activity NN 10_1101-2021_01_07_425723 705 27 . . . 10_1101-2021_01_07_425723 706 1 J. J. NNP 10_1101-2021_01_07_425723 706 2 Biol Biol NNP 10_1101-2021_01_07_425723 706 3 . . . 10_1101-2021_01_07_425723 707 1 Chem Chem NNP 10_1101-2021_01_07_425723 707 2 . . . 10_1101-2021_01_07_425723 708 1 286 286 CD 10_1101-2021_01_07_425723 708 2 , , , 10_1101-2021_01_07_425723 708 3 21835–821 21835–821 CD 10_1101-2021_01_07_425723 708 4 21843 21843 CD 10_1101-2021_01_07_425723 708 5 ( ( -LRB- 10_1101-2021_01_07_425723 708 6 2011 2011 CD 10_1101-2021_01_07_425723 708 7 ) ) -RRB- 10_1101-2021_01_07_425723 708 8 . . . 10_1101-2021_01_07_425723 709 1 822 822 CD 10_1101-2021_01_07_425723 709 2 45 45 CD 10_1101-2021_01_07_425723 709 3 . . . 10_1101-2021_01_07_425723 710 1 Takishita Takishita NNP 10_1101-2021_01_07_425723 710 2 , , , 10_1101-2021_01_07_425723 710 3 K. K. NNP 10_1101-2021_01_07_425723 710 4 et et NNP 10_1101-2021_01_07_425723 710 5 al al NNP 10_1101-2021_01_07_425723 710 6 . . . 10_1101-2021_01_07_425723 711 1 Lateral lateral JJ 10_1101-2021_01_07_425723 711 2 transfer transfer NN 10_1101-2021_01_07_425723 711 3 of of IN 10_1101-2021_01_07_425723 711 4 tetrahymanol tetrahymanol NN 10_1101-2021_01_07_425723 711 5 - - HYPH 10_1101-2021_01_07_425723 711 6 synthesizing synthesize VBG 10_1101-2021_01_07_425723 711 7 genes gene NNS 10_1101-2021_01_07_425723 711 8 has have VBZ 10_1101-2021_01_07_425723 711 9 allowed allow VBN 10_1101-2021_01_07_425723 711 10 multiple multiple JJ 10_1101-2021_01_07_425723 711 11 823 823 CD 10_1101-2021_01_07_425723 711 12 diverse diverse JJ 10_1101-2021_01_07_425723 711 13 eukaryote eukaryote NN 10_1101-2021_01_07_425723 711 14 lineages lineage NNS 10_1101-2021_01_07_425723 711 15 to to TO 10_1101-2021_01_07_425723 711 16 independently independently RB 10_1101-2021_01_07_425723 711 17 adapt adapt VB 10_1101-2021_01_07_425723 711 18 to to IN 10_1101-2021_01_07_425723 711 19 environments environment NNS 10_1101-2021_01_07_425723 711 20 without without IN 10_1101-2021_01_07_425723 711 21 oxygen oxygen NN 10_1101-2021_01_07_425723 711 22 . . . 10_1101-2021_01_07_425723 712 1 Biol Biol NNP 10_1101-2021_01_07_425723 712 2 . . . 10_1101-2021_01_07_425723 713 1 Direct direct JJ 10_1101-2021_01_07_425723 713 2 824 824 CD 10_1101-2021_01_07_425723 713 3 7 7 CD 10_1101-2021_01_07_425723 713 4 , , , 10_1101-2021_01_07_425723 713 5 5 5 CD 10_1101-2021_01_07_425723 713 6 ( ( -LRB- 10_1101-2021_01_07_425723 713 7 2012 2012 CD 10_1101-2021_01_07_425723 713 8 ) ) -RRB- 10_1101-2021_01_07_425723 713 9 . . . 10_1101-2021_01_07_425723 714 1 825 825 CD 10_1101-2021_01_07_425723 714 2 46 46 CD 10_1101-2021_01_07_425723 714 3 . . . 10_1101-2021_01_07_425723 715 1 Wiersma Wiersma NNP 10_1101-2021_01_07_425723 715 2 , , , 10_1101-2021_01_07_425723 715 3 S. S. NNP 10_1101-2021_01_07_425723 715 4 J. J. NNP 10_1101-2021_01_07_425723 715 5 , , , 10_1101-2021_01_07_425723 715 6 Mooiman Mooiman NNP 10_1101-2021_01_07_425723 715 7 , , , 10_1101-2021_01_07_425723 715 8 C. C. NNP 10_1101-2021_01_07_425723 715 9 , , , 10_1101-2021_01_07_425723 715 10 Giera Giera NNP 10_1101-2021_01_07_425723 715 11 , , , 10_1101-2021_01_07_425723 715 12 M. M. NNP 10_1101-2021_01_07_425723 715 13 & & CC 10_1101-2021_01_07_425723 715 14 Pronk Pronk NNP 10_1101-2021_01_07_425723 715 15 , , , 10_1101-2021_01_07_425723 715 16 J. J. NNP 10_1101-2021_01_07_425723 715 17 T. T. NNP 10_1101-2021_01_07_425723 715 18 Squalene Squalene NNP 10_1101-2021_01_07_425723 715 19 - - HYPH 10_1101-2021_01_07_425723 715 20 Tetrahymanol Tetrahymanol NNP 10_1101-2021_01_07_425723 715 21 Cyclase Cyclase NNP 10_1101-2021_01_07_425723 715 22 Expression expression NN 10_1101-2021_01_07_425723 715 23 826 826 CD 10_1101-2021_01_07_425723 715 24 Enables Enables NNP 10_1101-2021_01_07_425723 715 25 Sterol Sterol NNP 10_1101-2021_01_07_425723 715 26 - - HYPH 10_1101-2021_01_07_425723 715 27 Independent Independent NNP 10_1101-2021_01_07_425723 715 28 Growth Growth NNP 10_1101-2021_01_07_425723 715 29 of of IN 10_1101-2021_01_07_425723 715 30 Saccharomyces Saccharomyces NNPS 10_1101-2021_01_07_425723 715 31 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 715 32 . . . 10_1101-2021_01_07_425723 716 1 Appl Appl NNP 10_1101-2021_01_07_425723 716 2 . . . 10_1101-2021_01_07_425723 717 1 Environ Environ NNP 10_1101-2021_01_07_425723 717 2 . . . 10_1101-2021_01_07_425723 718 1 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 718 2 . . . 10_1101-2021_01_07_425723 719 1 86 86 CD 10_1101-2021_01_07_425723 719 2 , , , 10_1101-2021_01_07_425723 719 3 1–827 1–827 CD 10_1101-2021_01_07_425723 719 4 15 15 CD 10_1101-2021_01_07_425723 719 5 ( ( -LRB- 10_1101-2021_01_07_425723 719 6 2020 2020 CD 10_1101-2021_01_07_425723 719 7 ) ) -RRB- 10_1101-2021_01_07_425723 719 8 . . . 10_1101-2021_01_07_425723 720 1 828 828 CD 10_1101-2021_01_07_425723 720 2 47 47 CD 10_1101-2021_01_07_425723 720 3 . . . 10_1101-2021_01_07_425723 721 1 Rajkumar Rajkumar NNP 10_1101-2021_01_07_425723 721 2 , , , 10_1101-2021_01_07_425723 721 3 A. a. NN 10_1101-2021_01_07_425723 721 4 S. S. NNP 10_1101-2021_01_07_425723 721 5 , , , 10_1101-2021_01_07_425723 721 6 Varela Varela NNP 10_1101-2021_01_07_425723 721 7 , , , 10_1101-2021_01_07_425723 721 8 J. J. NNP 10_1101-2021_01_07_425723 722 1 A. A. NNP 10_1101-2021_01_07_425723 722 2 , , , 10_1101-2021_01_07_425723 722 3 Juergens Juergens NNP 10_1101-2021_01_07_425723 722 4 , , , 10_1101-2021_01_07_425723 722 5 H. H. NNP 10_1101-2021_01_07_425723 722 6 , , , 10_1101-2021_01_07_425723 722 7 Daran Daran NNP 10_1101-2021_01_07_425723 722 8 , , , 10_1101-2021_01_07_425723 722 9 J. J. NNP 10_1101-2021_01_07_425723 722 10 G. G. NNP 10_1101-2021_01_07_425723 722 11 & & CC 10_1101-2021_01_07_425723 722 12 Morrissey Morrissey NNP 10_1101-2021_01_07_425723 722 13 , , , 10_1101-2021_01_07_425723 722 14 J. J. NNP 10_1101-2021_01_07_425723 722 15 P. P. NNP 10_1101-2021_01_07_425723 722 16 Biological Biological NNP 10_1101-2021_01_07_425723 722 17 Parts Parts NNPS 10_1101-2021_01_07_425723 722 18 for for IN 10_1101-2021_01_07_425723 722 19 829 829 CD 10_1101-2021_01_07_425723 722 20 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 722 21 marxianus marxianus NN 10_1101-2021_01_07_425723 722 22 Synthetic Synthetic NNP 10_1101-2021_01_07_425723 722 23 Biology biology NN 10_1101-2021_01_07_425723 722 24 . . . 10_1101-2021_01_07_425723 723 1 Front front NN 10_1101-2021_01_07_425723 723 2 . . . 10_1101-2021_01_07_425723 724 1 Bioeng Bioeng NNP 10_1101-2021_01_07_425723 724 2 . . . 10_1101-2021_01_07_425723 725 1 Biotechnol Biotechnol NNS 10_1101-2021_01_07_425723 725 2 . . . 10_1101-2021_01_07_425723 726 1 7 7 LS 10_1101-2021_01_07_425723 726 2 , , , 10_1101-2021_01_07_425723 726 3 1–15 1–15 CD 10_1101-2021_01_07_425723 726 4 ( ( -LRB- 10_1101-2021_01_07_425723 726 5 2019 2019 CD 10_1101-2021_01_07_425723 726 6 ) ) -RRB- 10_1101-2021_01_07_425723 726 7 . . . 10_1101-2021_01_07_425723 727 1 830 830 CD 10_1101-2021_01_07_425723 727 2 48 48 CD 10_1101-2021_01_07_425723 727 3 . . . 10_1101-2021_01_07_425723 728 1 Landry Landry NNP 10_1101-2021_01_07_425723 728 2 , , , 10_1101-2021_01_07_425723 728 3 B. B. NNP 10_1101-2021_01_07_425723 728 4 D. D. NNP 10_1101-2021_01_07_425723 728 5 , , , 10_1101-2021_01_07_425723 728 6 Doyle Doyle NNP 10_1101-2021_01_07_425723 728 7 , , , 10_1101-2021_01_07_425723 728 8 J. J. NNP 10_1101-2021_01_07_425723 728 9 P. P. NNP 10_1101-2021_01_07_425723 728 10 , , , 10_1101-2021_01_07_425723 728 11 Toczyski Toczyski NNP 10_1101-2021_01_07_425723 728 12 , , , 10_1101-2021_01_07_425723 728 13 D. D. NNP 10_1101-2021_01_07_425723 728 14 P. P. NNP 10_1101-2021_01_07_425723 728 15 & & CC 10_1101-2021_01_07_425723 728 16 Benanti Benanti NNP 10_1101-2021_01_07_425723 728 17 , , , 10_1101-2021_01_07_425723 728 18 J. J. NNP 10_1101-2021_01_07_425723 729 1 A. A. NNP 10_1101-2021_01_07_425723 729 2 F F NNP 10_1101-2021_01_07_425723 729 3 - - HYPH 10_1101-2021_01_07_425723 729 4 Box Box NNP 10_1101-2021_01_07_425723 729 5 Protein Protein NNP 10_1101-2021_01_07_425723 729 6 Specificity Specificity NNP 10_1101-2021_01_07_425723 729 7 for for IN 10_1101-2021_01_07_425723 729 8 G1 G1 NNP 10_1101-2021_01_07_425723 729 9 Cyclins Cyclins NNP 10_1101-2021_01_07_425723 729 10 Is be VBZ 10_1101-2021_01_07_425723 729 11 831 831 CD 10_1101-2021_01_07_425723 729 12 Dictated dictate VBN 10_1101-2021_01_07_425723 729 13 by by IN 10_1101-2021_01_07_425723 729 14 Subcellular Subcellular NNP 10_1101-2021_01_07_425723 729 15 Localization Localization NNP 10_1101-2021_01_07_425723 729 16 . . . 10_1101-2021_01_07_425723 730 1 PLoS PLoS NNP 10_1101-2021_01_07_425723 730 2 Genet Genet NNP 10_1101-2021_01_07_425723 730 3 . . . 10_1101-2021_01_07_425723 731 1 8 8 CD 10_1101-2021_01_07_425723 731 2 , , , 10_1101-2021_01_07_425723 731 3 e1002851 e1002851 NNP 10_1101-2021_01_07_425723 731 4 ( ( -LRB- 10_1101-2021_01_07_425723 731 5 2012 2012 CD 10_1101-2021_01_07_425723 731 6 ) ) -RRB- 10_1101-2021_01_07_425723 731 7 . . . 10_1101-2021_01_07_425723 732 1 832 832 CD 10_1101-2021_01_07_425723 732 2 49 49 CD 10_1101-2021_01_07_425723 732 3 . . . 10_1101-2021_01_07_425723 733 1 Fonseca Fonseca NNP 10_1101-2021_01_07_425723 733 2 , , , 10_1101-2021_01_07_425723 733 3 G. G. NNP 10_1101-2021_01_07_425723 733 4 G. G. NNP 10_1101-2021_01_07_425723 733 5 , , , 10_1101-2021_01_07_425723 733 6 Heinzle Heinzle NNP 10_1101-2021_01_07_425723 733 7 , , , 10_1101-2021_01_07_425723 733 8 E. E. NNP 10_1101-2021_01_07_425723 733 9 , , , 10_1101-2021_01_07_425723 733 10 Wittmann Wittmann NNP 10_1101-2021_01_07_425723 733 11 , , , 10_1101-2021_01_07_425723 733 12 C. C. NNP 10_1101-2021_01_07_425723 733 13 & & CC 10_1101-2021_01_07_425723 733 14 Gombert Gombert NNP 10_1101-2021_01_07_425723 733 15 , , , 10_1101-2021_01_07_425723 733 16 A. A. NNP 10_1101-2021_01_07_425723 733 17 K. K. NNP 10_1101-2021_01_07_425723 733 18 The the DT 10_1101-2021_01_07_425723 733 19 yeast yeast NN 10_1101-2021_01_07_425723 733 20 Kluyveromyces kluyveromyce VBZ 10_1101-2021_01_07_425723 733 21 marxianus marxianus NN 10_1101-2021_01_07_425723 733 22 833 833 CD 10_1101-2021_01_07_425723 733 23 and and CC 10_1101-2021_01_07_425723 733 24 its -PRON- PRP$ 10_1101-2021_01_07_425723 733 25 biotechnological biotechnological JJ 10_1101-2021_01_07_425723 733 26 potential potential NN 10_1101-2021_01_07_425723 733 27 . . . 10_1101-2021_01_07_425723 734 1 Appl Appl NNP 10_1101-2021_01_07_425723 734 2 . . . 10_1101-2021_01_07_425723 735 1 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 735 2 . . . 10_1101-2021_01_07_425723 736 1 Biotechnol Biotechnol NNS 10_1101-2021_01_07_425723 736 2 . . . 10_1101-2021_01_07_425723 737 1 79 79 CD 10_1101-2021_01_07_425723 737 2 , , , 10_1101-2021_01_07_425723 737 3 339–354 339–354 CD 10_1101-2021_01_07_425723 737 4 ( ( -LRB- 10_1101-2021_01_07_425723 737 5 2008 2008 CD 10_1101-2021_01_07_425723 737 6 ) ) -RRB- 10_1101-2021_01_07_425723 737 7 . . . 10_1101-2021_01_07_425723 738 1 834 834 CD 10_1101-2021_01_07_425723 738 2 50 50 CD 10_1101-2021_01_07_425723 738 3 . . . 10_1101-2021_01_07_425723 739 1 Madeira Madeira NNP 10_1101-2021_01_07_425723 739 2 - - HYPH 10_1101-2021_01_07_425723 739 3 Jr Jr NNP 10_1101-2021_01_07_425723 739 4 , , , 10_1101-2021_01_07_425723 739 5 J. J. NNP 10_1101-2021_01_07_425723 739 6 V. V. NNP 10_1101-2021_01_07_425723 739 7 & & CC 10_1101-2021_01_07_425723 739 8 Gombert Gombert NNP 10_1101-2021_01_07_425723 739 9 , , , 10_1101-2021_01_07_425723 739 10 A. A. NNP 10_1101-2021_01_07_425723 739 11 K. K. NNP 10_1101-2021_01_07_425723 739 12 Towards towards IN 10_1101-2021_01_07_425723 739 13 high high JJ 10_1101-2021_01_07_425723 739 14 - - HYPH 10_1101-2021_01_07_425723 739 15 temperature temperature NN 10_1101-2021_01_07_425723 739 16 fuel fuel NN 10_1101-2021_01_07_425723 739 17 ethanol ethanol NN 10_1101-2021_01_07_425723 739 18 production production NN 10_1101-2021_01_07_425723 739 19 using use VBG 10_1101-2021_01_07_425723 739 20 835 835 CD 10_1101-2021_01_07_425723 739 21 .CC .CC , 10_1101-2021_01_07_425723 739 22 - - : 10_1101-2021_01_07_425723 739 23 BY by IN 10_1101-2021_01_07_425723 739 24 - - HYPH 10_1101-2021_01_07_425723 739 25 NC NC NNP 10_1101-2021_01_07_425723 739 26 - - HYPH 10_1101-2021_01_07_425723 739 27 ND ND NNP 10_1101-2021_01_07_425723 739 28 4.0 4.0 CD 10_1101-2021_01_07_425723 739 29 International International NNP 10_1101-2021_01_07_425723 739 30 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 739 31 under under IN 10_1101-2021_01_07_425723 739 32 a a DT 10_1101-2021_01_07_425723 739 33 ( ( -LRB- 10_1101-2021_01_07_425723 739 34 which which WDT 10_1101-2021_01_07_425723 739 35 was be VBD 10_1101-2021_01_07_425723 739 36 not not RB 10_1101-2021_01_07_425723 739 37 certified certify VBN 10_1101-2021_01_07_425723 739 38 by by IN 10_1101-2021_01_07_425723 739 39 peer peer NN 10_1101-2021_01_07_425723 739 40 review review NN 10_1101-2021_01_07_425723 739 41 ) ) -RRB- 10_1101-2021_01_07_425723 739 42 is be VBZ 10_1101-2021_01_07_425723 739 43 the the DT 10_1101-2021_01_07_425723 739 44 author author NN 10_1101-2021_01_07_425723 739 45 / / SYM 10_1101-2021_01_07_425723 739 46 funder funder NN 10_1101-2021_01_07_425723 739 47 , , , 10_1101-2021_01_07_425723 739 48 who who WP 10_1101-2021_01_07_425723 739 49 has have VBZ 10_1101-2021_01_07_425723 739 50 granted grant VBN 10_1101-2021_01_07_425723 739 51 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 739 52 a a DT 10_1101-2021_01_07_425723 739 53 license license NN 10_1101-2021_01_07_425723 739 54 to to TO 10_1101-2021_01_07_425723 739 55 display display VB 10_1101-2021_01_07_425723 739 56 the the DT 10_1101-2021_01_07_425723 739 57 preprint preprint NN 10_1101-2021_01_07_425723 739 58 in in IN 10_1101-2021_01_07_425723 739 59 perpetuity perpetuity NN 10_1101-2021_01_07_425723 739 60 . . . 10_1101-2021_01_07_425723 740 1 It -PRON- PRP 10_1101-2021_01_07_425723 740 2 is be VBZ 10_1101-2021_01_07_425723 740 3 made make VBN 10_1101-2021_01_07_425723 740 4 The the DT 10_1101-2021_01_07_425723 740 5 copyright copyright NN 10_1101-2021_01_07_425723 740 6 holder holder NN 10_1101-2021_01_07_425723 740 7 for for IN 10_1101-2021_01_07_425723 740 8 this this DT 10_1101-2021_01_07_425723 740 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 740 10 version version NN 10_1101-2021_01_07_425723 740 11 posted post VBD 10_1101-2021_01_07_425723 740 12 January January NNP 10_1101-2021_01_07_425723 740 13 8 8 CD 10_1101-2021_01_07_425723 740 14 , , , 10_1101-2021_01_07_425723 740 15 2021 2021 CD 10_1101-2021_01_07_425723 740 16 . . . 10_1101-2021_01_07_425723 740 17 ; ; : 10_1101-2021_01_07_425723 740 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 740 19 : : : 10_1101-2021_01_07_425723 740 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 740 21 preprint preprint NN 10_1101-2021_01_07_425723 740 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 740 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425723 740 24 43 43 CD 10_1101-2021_01_07_425723 740 25 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 740 26 marxianus marxianus NN 10_1101-2021_01_07_425723 740 27 : : : 10_1101-2021_01_07_425723 740 28 On on IN 10_1101-2021_01_07_425723 740 29 the the DT 10_1101-2021_01_07_425723 740 30 search search NN 10_1101-2021_01_07_425723 740 31 for for IN 10_1101-2021_01_07_425723 740 32 plug plug NN 10_1101-2021_01_07_425723 740 33 - - HYPH 10_1101-2021_01_07_425723 740 34 in in RP 10_1101-2021_01_07_425723 740 35 strains strain NNS 10_1101-2021_01_07_425723 740 36 for for IN 10_1101-2021_01_07_425723 740 37 the the DT 10_1101-2021_01_07_425723 740 38 Brazilian brazilian JJ 10_1101-2021_01_07_425723 740 39 sugarcane sugarcane NN 10_1101-2021_01_07_425723 740 40 - - HYPH 10_1101-2021_01_07_425723 740 41 based base VBN 10_1101-2021_01_07_425723 740 42 836 836 CD 10_1101-2021_01_07_425723 740 43 biorefinery biorefinery NN 10_1101-2021_01_07_425723 740 44 . . . 10_1101-2021_01_07_425723 741 1 Biomass Biomass NNP 10_1101-2021_01_07_425723 741 2 and and CC 10_1101-2021_01_07_425723 741 3 Bioenergy Bioenergy NNP 10_1101-2021_01_07_425723 741 4 119 119 CD 10_1101-2021_01_07_425723 741 5 , , , 10_1101-2021_01_07_425723 741 6 217–228 217–228 CD 10_1101-2021_01_07_425723 741 7 ( ( -LRB- 10_1101-2021_01_07_425723 741 8 2018 2018 CD 10_1101-2021_01_07_425723 741 9 ) ) -RRB- 10_1101-2021_01_07_425723 741 10 . . . 10_1101-2021_01_07_425723 742 1 837 837 CD 10_1101-2021_01_07_425723 742 2 51 51 CD 10_1101-2021_01_07_425723 742 3 . . . 10_1101-2021_01_07_425723 743 1 Tai Tai NNP 10_1101-2021_01_07_425723 743 2 , , , 10_1101-2021_01_07_425723 743 3 S. S. NNP 10_1101-2021_01_07_425723 743 4 L. L. NNP 10_1101-2021_01_07_425723 743 5 et et NNP 10_1101-2021_01_07_425723 743 6 al al NNP 10_1101-2021_01_07_425723 743 7 . . . 10_1101-2021_01_07_425723 744 1 Two two CD 10_1101-2021_01_07_425723 744 2 - - HYPH 10_1101-2021_01_07_425723 744 3 dimensional dimensional JJ 10_1101-2021_01_07_425723 744 4 transcriptome transcriptome NNP 10_1101-2021_01_07_425723 744 5 analysis analysis NN 10_1101-2021_01_07_425723 744 6 in in IN 10_1101-2021_01_07_425723 744 7 chemostat chemostat JJ 10_1101-2021_01_07_425723 744 8 cultures culture NNS 10_1101-2021_01_07_425723 744 9 : : : 10_1101-2021_01_07_425723 744 10 Combinatorial combinatorial NN 10_1101-2021_01_07_425723 744 11 838 838 CD 10_1101-2021_01_07_425723 744 12 effects effect NNS 10_1101-2021_01_07_425723 744 13 of of IN 10_1101-2021_01_07_425723 744 14 oxygen oxygen NN 10_1101-2021_01_07_425723 744 15 availability availability NN 10_1101-2021_01_07_425723 744 16 and and CC 10_1101-2021_01_07_425723 744 17 macronutrient macronutrient NN 10_1101-2021_01_07_425723 744 18 limitation limitation NN 10_1101-2021_01_07_425723 744 19 in in IN 10_1101-2021_01_07_425723 744 20 Saccharomyces Saccharomyces NNP 10_1101-2021_01_07_425723 744 21 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 744 22 . . . 10_1101-2021_01_07_425723 745 1 J. J. NNP 10_1101-2021_01_07_425723 745 2 Biol Biol NNP 10_1101-2021_01_07_425723 745 3 . . . 10_1101-2021_01_07_425723 746 1 839 839 CD 10_1101-2021_01_07_425723 746 2 Chem Chem NNP 10_1101-2021_01_07_425723 746 3 . . . 10_1101-2021_01_07_425723 747 1 280 280 CD 10_1101-2021_01_07_425723 747 2 , , , 10_1101-2021_01_07_425723 747 3 437–447 437–447 CD 10_1101-2021_01_07_425723 747 4 ( ( -LRB- 10_1101-2021_01_07_425723 747 5 2005 2005 CD 10_1101-2021_01_07_425723 747 6 ) ) -RRB- 10_1101-2021_01_07_425723 747 7 . . . 10_1101-2021_01_07_425723 748 1 840 840 CD 10_1101-2021_01_07_425723 748 2 52 52 CD 10_1101-2021_01_07_425723 748 3 . . . 10_1101-2021_01_07_425723 749 1 Seret Seret NNP 10_1101-2021_01_07_425723 749 2 , , , 10_1101-2021_01_07_425723 749 3 M. M. NNP 10_1101-2021_01_07_425723 749 4 L. L. NNP 10_1101-2021_01_07_425723 749 5 , , , 10_1101-2021_01_07_425723 749 6 Diffels Diffels NNP 10_1101-2021_01_07_425723 749 7 , , , 10_1101-2021_01_07_425723 749 8 J. J. NNP 10_1101-2021_01_07_425723 749 9 F. F. NNP 10_1101-2021_01_07_425723 749 10 , , , 10_1101-2021_01_07_425723 749 11 Goffeau Goffeau NNP 10_1101-2021_01_07_425723 749 12 , , , 10_1101-2021_01_07_425723 749 13 A. a. NN 10_1101-2021_01_07_425723 750 1 & & CC 10_1101-2021_01_07_425723 750 2 Baret Baret NNP 10_1101-2021_01_07_425723 750 3 , , , 10_1101-2021_01_07_425723 750 4 P. P. NNP 10_1101-2021_01_07_425723 750 5 V. V. NNP 10_1101-2021_01_07_425723 750 6 Combined Combined NNP 10_1101-2021_01_07_425723 750 7 phylogeny phylogeny NN 10_1101-2021_01_07_425723 750 8 and and CC 10_1101-2021_01_07_425723 750 9 neighborhood neighborhood NN 10_1101-2021_01_07_425723 750 10 841 841 CD 10_1101-2021_01_07_425723 750 11 analysis analysis NN 10_1101-2021_01_07_425723 750 12 of of IN 10_1101-2021_01_07_425723 750 13 the the DT 10_1101-2021_01_07_425723 750 14 evolution evolution NN 10_1101-2021_01_07_425723 750 15 of of IN 10_1101-2021_01_07_425723 750 16 the the DT 10_1101-2021_01_07_425723 750 17 ABC ABC NNP 10_1101-2021_01_07_425723 750 18 transporters transporter NNS 10_1101-2021_01_07_425723 750 19 conferring confer VBG 10_1101-2021_01_07_425723 750 20 multiple multiple JJ 10_1101-2021_01_07_425723 750 21 drug drug NN 10_1101-2021_01_07_425723 750 22 resistance resistance NN 10_1101-2021_01_07_425723 750 23 in in IN 10_1101-2021_01_07_425723 750 24 842 842 CD 10_1101-2021_01_07_425723 750 25 hemiascomycete hemiascomycete JJ 10_1101-2021_01_07_425723 750 26 yeasts yeast NNS 10_1101-2021_01_07_425723 750 27 . . . 10_1101-2021_01_07_425723 751 1 BMC BMC NNP 10_1101-2021_01_07_425723 751 2 Genomics Genomics NNP 10_1101-2021_01_07_425723 751 3 10 10 CD 10_1101-2021_01_07_425723 751 4 , , , 10_1101-2021_01_07_425723 751 5 459 459 CD 10_1101-2021_01_07_425723 751 6 ( ( -LRB- 10_1101-2021_01_07_425723 751 7 2009 2009 CD 10_1101-2021_01_07_425723 751 8 ) ) -RRB- 10_1101-2021_01_07_425723 751 9 . . . 10_1101-2021_01_07_425723 752 1 843 843 CD 10_1101-2021_01_07_425723 752 2 53 53 CD 10_1101-2021_01_07_425723 752 3 . . . 10_1101-2021_01_07_425723 753 1 Shi Shi NNP 10_1101-2021_01_07_425723 753 2 , , , 10_1101-2021_01_07_425723 753 3 N. N. NNP 10_1101-2021_01_07_425723 753 4 Q. Q. NNP 10_1101-2021_01_07_425723 754 1 & & CC 10_1101-2021_01_07_425723 754 2 Jeffries Jeffries NNP 10_1101-2021_01_07_425723 754 3 , , , 10_1101-2021_01_07_425723 754 4 T. T. NNP 10_1101-2021_01_07_425723 754 5 W. w. NN 10_1101-2021_01_07_425723 754 6 Anaerobic anaerobic JJ 10_1101-2021_01_07_425723 754 7 growth growth NN 10_1101-2021_01_07_425723 754 8 and and CC 10_1101-2021_01_07_425723 754 9 improved improved JJ 10_1101-2021_01_07_425723 754 10 fermentation fermentation NN 10_1101-2021_01_07_425723 754 11 of of IN 10_1101-2021_01_07_425723 754 12 Pichia Pichia NNP 10_1101-2021_01_07_425723 754 13 stipitis stipitis NN 10_1101-2021_01_07_425723 754 14 bearing bear VBG 10_1101-2021_01_07_425723 754 15 844 844 CD 10_1101-2021_01_07_425723 754 16 a a DT 10_1101-2021_01_07_425723 754 17 URA1 URA1 NNP 10_1101-2021_01_07_425723 754 18 gene gene NN 10_1101-2021_01_07_425723 754 19 from from IN 10_1101-2021_01_07_425723 754 20 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 754 21 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 754 22 . . . 10_1101-2021_01_07_425723 755 1 Appl Appl NNP 10_1101-2021_01_07_425723 755 2 . . . 10_1101-2021_01_07_425723 756 1 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 756 2 . . . 10_1101-2021_01_07_425723 757 1 Biotechnol Biotechnol NNS 10_1101-2021_01_07_425723 757 2 . . . 10_1101-2021_01_07_425723 758 1 50 50 CD 10_1101-2021_01_07_425723 758 2 , , , 10_1101-2021_01_07_425723 758 3 339–345 339–345 CD 10_1101-2021_01_07_425723 758 4 ( ( -LRB- 10_1101-2021_01_07_425723 758 5 1998 1998 CD 10_1101-2021_01_07_425723 758 6 ) ) -RRB- 10_1101-2021_01_07_425723 758 7 . . . 10_1101-2021_01_07_425723 759 1 845 845 CD 10_1101-2021_01_07_425723 759 2 54 54 CD 10_1101-2021_01_07_425723 759 3 . . . 10_1101-2021_01_07_425723 760 1 Gojković Gojković NNP 10_1101-2021_01_07_425723 760 2 , , , 10_1101-2021_01_07_425723 760 3 Z. Z. NNP 10_1101-2021_01_07_425723 760 4 et et FW 10_1101-2021_01_07_425723 760 5 al al NNP 10_1101-2021_01_07_425723 760 6 . . . 10_1101-2021_01_07_425723 761 1 Horizontal horizontal JJ 10_1101-2021_01_07_425723 761 2 gene gene NN 10_1101-2021_01_07_425723 761 3 transfer transfer NN 10_1101-2021_01_07_425723 761 4 promoted promote VBD 10_1101-2021_01_07_425723 761 5 evolution evolution NN 10_1101-2021_01_07_425723 761 6 of of IN 10_1101-2021_01_07_425723 761 7 the the DT 10_1101-2021_01_07_425723 761 8 ability ability NN 10_1101-2021_01_07_425723 761 9 to to TO 10_1101-2021_01_07_425723 761 10 propagate propagate VB 10_1101-2021_01_07_425723 761 11 under under IN 10_1101-2021_01_07_425723 761 12 846 846 CD 10_1101-2021_01_07_425723 761 13 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 761 14 conditions condition NNS 10_1101-2021_01_07_425723 761 15 in in IN 10_1101-2021_01_07_425723 761 16 yeasts yeast NNS 10_1101-2021_01_07_425723 761 17 . . . 10_1101-2021_01_07_425723 762 1 Mol Mol NNP 10_1101-2021_01_07_425723 762 2 . . . 10_1101-2021_01_07_425723 763 1 Genet Genet NNP 10_1101-2021_01_07_425723 763 2 . . . 10_1101-2021_01_07_425723 764 1 Genomics Genomics NNP 10_1101-2021_01_07_425723 764 2 271 271 CD 10_1101-2021_01_07_425723 764 3 , , , 10_1101-2021_01_07_425723 764 4 387–393 387–393 CD 10_1101-2021_01_07_425723 764 5 ( ( -LRB- 10_1101-2021_01_07_425723 764 6 2004 2004 CD 10_1101-2021_01_07_425723 764 7 ) ) -RRB- 10_1101-2021_01_07_425723 764 8 . . . 10_1101-2021_01_07_425723 765 1 847 847 CD 10_1101-2021_01_07_425723 765 2 55 55 CD 10_1101-2021_01_07_425723 765 3 . . . 10_1101-2021_01_07_425723 766 1 Riley Riley NNP 10_1101-2021_01_07_425723 766 2 , , , 10_1101-2021_01_07_425723 766 3 R. R. NNP 10_1101-2021_01_07_425723 766 4 et et NNP 10_1101-2021_01_07_425723 766 5 al al NNP 10_1101-2021_01_07_425723 766 6 . . . 10_1101-2021_01_07_425723 767 1 Comparative comparative JJ 10_1101-2021_01_07_425723 767 2 genomics genomic NNS 10_1101-2021_01_07_425723 767 3 of of IN 10_1101-2021_01_07_425723 767 4 biotechnologically biotechnologically RB 10_1101-2021_01_07_425723 767 5 important important JJ 10_1101-2021_01_07_425723 767 6 yeasts yeast NNS 10_1101-2021_01_07_425723 767 7 . . . 10_1101-2021_01_07_425723 768 1 Proc Proc NNP 10_1101-2021_01_07_425723 768 2 . . . 10_1101-2021_01_07_425723 769 1 Natl Natl NNP 10_1101-2021_01_07_425723 769 2 . . . 10_1101-2021_01_07_425723 770 1 Acad Acad NNS 10_1101-2021_01_07_425723 770 2 . . . 10_1101-2021_01_07_425723 771 1 Sci Sci NNP 10_1101-2021_01_07_425723 771 2 . . . 10_1101-2021_01_07_425723 772 1 848 848 CD 10_1101-2021_01_07_425723 772 2 U. U. NNP 10_1101-2021_01_07_425723 772 3 S. S. NNP 10_1101-2021_01_07_425723 772 4 A. a. NN 10_1101-2021_01_07_425723 773 1 113 113 CD 10_1101-2021_01_07_425723 773 2 , , , 10_1101-2021_01_07_425723 773 3 9882–9887 9882–9887 CD 10_1101-2021_01_07_425723 773 4 ( ( -LRB- 10_1101-2021_01_07_425723 773 5 2016 2016 CD 10_1101-2021_01_07_425723 773 6 ) ) -RRB- 10_1101-2021_01_07_425723 773 7 . . . 10_1101-2021_01_07_425723 774 1 849 849 CD 10_1101-2021_01_07_425723 774 2 56 56 CD 10_1101-2021_01_07_425723 774 3 . . . 10_1101-2021_01_07_425723 775 1 Guo Guo NNP 10_1101-2021_01_07_425723 775 2 , , , 10_1101-2021_01_07_425723 775 3 L. L. NNP 10_1101-2021_01_07_425723 775 4 , , , 10_1101-2021_01_07_425723 775 5 Pang Pang NNP 10_1101-2021_01_07_425723 775 6 , , , 10_1101-2021_01_07_425723 775 7 Z. Z. NNP 10_1101-2021_01_07_425723 775 8 , , , 10_1101-2021_01_07_425723 775 9 Gao Gao NNP 10_1101-2021_01_07_425723 775 10 , , , 10_1101-2021_01_07_425723 775 11 C. C. NNP 10_1101-2021_01_07_425723 775 12 , , , 10_1101-2021_01_07_425723 775 13 Chen Chen NNP 10_1101-2021_01_07_425723 775 14 , , , 10_1101-2021_01_07_425723 775 15 X. X. NNP 10_1101-2021_01_07_425723 776 1 & & CC 10_1101-2021_01_07_425723 776 2 Liu Liu NNP 10_1101-2021_01_07_425723 776 3 , , , 10_1101-2021_01_07_425723 776 4 L. L. NNP 10_1101-2021_01_07_425723 776 5 Engineering Engineering NNP 10_1101-2021_01_07_425723 776 6 microbial microbial JJ 10_1101-2021_01_07_425723 776 7 cell cell NN 10_1101-2021_01_07_425723 776 8 morphology morphology NN 10_1101-2021_01_07_425723 776 9 and and CC 10_1101-2021_01_07_425723 776 10 membrane membrane NN 10_1101-2021_01_07_425723 776 11 850 850 CD 10_1101-2021_01_07_425723 776 12 homeostasis homeostasis NN 10_1101-2021_01_07_425723 776 13 toward toward IN 10_1101-2021_01_07_425723 776 14 industrial industrial JJ 10_1101-2021_01_07_425723 776 15 applications application NNS 10_1101-2021_01_07_425723 776 16 . . . 10_1101-2021_01_07_425723 777 1 Curr curr UH 10_1101-2021_01_07_425723 777 2 . . . 10_1101-2021_01_07_425723 778 1 Opin opin JJ 10_1101-2021_01_07_425723 778 2 . . . 10_1101-2021_01_07_425723 779 1 Biotechnol Biotechnol NNS 10_1101-2021_01_07_425723 779 2 . . . 10_1101-2021_01_07_425723 780 1 66 66 CD 10_1101-2021_01_07_425723 780 2 , , , 10_1101-2021_01_07_425723 780 3 18–26 18–26 CD 10_1101-2021_01_07_425723 780 4 ( ( -LRB- 10_1101-2021_01_07_425723 780 5 2020 2020 CD 10_1101-2021_01_07_425723 780 6 ) ) -RRB- 10_1101-2021_01_07_425723 780 7 . . . 10_1101-2021_01_07_425723 781 1 851 851 CD 10_1101-2021_01_07_425723 781 2 57 57 CD 10_1101-2021_01_07_425723 781 3 . . . 10_1101-2021_01_07_425723 782 1 Entian Entian NNP 10_1101-2021_01_07_425723 782 2 , , , 10_1101-2021_01_07_425723 782 3 K.-D. K.-D. NNP 10_1101-2021_01_07_425723 782 4 & & CC 10_1101-2021_01_07_425723 782 5 Kötter Kötter NNP 10_1101-2021_01_07_425723 782 6 , , , 10_1101-2021_01_07_425723 782 7 P. P. NNP 10_1101-2021_01_07_425723 782 8 25 25 CD 10_1101-2021_01_07_425723 782 9 Yeast Yeast NNP 10_1101-2021_01_07_425723 782 10 Genetic Genetic NNP 10_1101-2021_01_07_425723 782 11 Strain Strain NNP 10_1101-2021_01_07_425723 782 12 and and CC 10_1101-2021_01_07_425723 782 13 Plasmid Plasmid NNP 10_1101-2021_01_07_425723 782 14 Collections Collections NNPS 10_1101-2021_01_07_425723 782 15 . . . 10_1101-2021_01_07_425723 783 1 in in IN 10_1101-2021_01_07_425723 783 2 Methods method NNS 10_1101-2021_01_07_425723 783 3 in in IN 10_1101-2021_01_07_425723 783 4 852 852 CD 10_1101-2021_01_07_425723 783 5 Microbiology Microbiology NNP 10_1101-2021_01_07_425723 783 6 629–666 629–666 CD 10_1101-2021_01_07_425723 783 7 ( ( -LRB- 10_1101-2021_01_07_425723 783 8 2007 2007 CD 10_1101-2021_01_07_425723 783 9 ) ) -RRB- 10_1101-2021_01_07_425723 783 10 . . . 10_1101-2021_01_07_425723 784 1 doi:10.1016 doi:10.1016 JJ 10_1101-2021_01_07_425723 784 2 / / SYM 10_1101-2021_01_07_425723 784 3 S0580 S0580 NNP 10_1101-2021_01_07_425723 784 4 - - HYPH 10_1101-2021_01_07_425723 784 5 9517(06)36025 9517(06)36025 NNP 10_1101-2021_01_07_425723 784 6 - - HYPH 10_1101-2021_01_07_425723 784 7 4 4 CD 10_1101-2021_01_07_425723 784 8 853 853 CD 10_1101-2021_01_07_425723 784 9 58 58 CD 10_1101-2021_01_07_425723 784 10 . . . 10_1101-2021_01_07_425723 785 1 Nijkamp Nijkamp NNP 10_1101-2021_01_07_425723 785 2 , , , 10_1101-2021_01_07_425723 785 3 J. J. NNP 10_1101-2021_01_07_425723 785 4 F. F. NNP 10_1101-2021_01_07_425723 785 5 et et NNP 10_1101-2021_01_07_425723 785 6 al al NNP 10_1101-2021_01_07_425723 785 7 . . . 10_1101-2021_01_07_425723 786 1 De De NNP 10_1101-2021_01_07_425723 786 2 novo novo NNP 10_1101-2021_01_07_425723 786 3 sequencing sequencing NN 10_1101-2021_01_07_425723 786 4 , , , 10_1101-2021_01_07_425723 786 5 assembly assembly NN 10_1101-2021_01_07_425723 786 6 and and CC 10_1101-2021_01_07_425723 786 7 analysis analysis NN 10_1101-2021_01_07_425723 786 8 of of IN 10_1101-2021_01_07_425723 786 9 the the DT 10_1101-2021_01_07_425723 786 10 genome genome NN 10_1101-2021_01_07_425723 786 11 of of IN 10_1101-2021_01_07_425723 786 12 the the DT 10_1101-2021_01_07_425723 786 13 laboratory laboratory NN 10_1101-2021_01_07_425723 786 14 854 854 CD 10_1101-2021_01_07_425723 786 15 strain strain NN 10_1101-2021_01_07_425723 786 16 Saccharomyces Saccharomyces NNPS 10_1101-2021_01_07_425723 786 17 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 786 18 CEN.PK113 CEN.PK113 NNP 10_1101-2021_01_07_425723 786 19 - - HYPH 10_1101-2021_01_07_425723 786 20 7D 7D NNP 10_1101-2021_01_07_425723 786 21 , , , 10_1101-2021_01_07_425723 786 22 a a DT 10_1101-2021_01_07_425723 786 23 model model NN 10_1101-2021_01_07_425723 786 24 for for IN 10_1101-2021_01_07_425723 786 25 modern modern JJ 10_1101-2021_01_07_425723 786 26 industrial industrial JJ 10_1101-2021_01_07_425723 786 27 biotechnology biotechnology NN 10_1101-2021_01_07_425723 786 28 . . . 10_1101-2021_01_07_425723 787 1 855 855 CD 10_1101-2021_01_07_425723 787 2 Microb Microb NNP 10_1101-2021_01_07_425723 787 3 . . . 10_1101-2021_01_07_425723 788 1 Cell Cell NNP 10_1101-2021_01_07_425723 788 2 Fact Fact NNP 10_1101-2021_01_07_425723 788 3 . . . 10_1101-2021_01_07_425723 789 1 11 11 CD 10_1101-2021_01_07_425723 789 2 , , , 10_1101-2021_01_07_425723 789 3 36 36 CD 10_1101-2021_01_07_425723 789 4 ( ( -LRB- 10_1101-2021_01_07_425723 789 5 2012 2012 CD 10_1101-2021_01_07_425723 789 6 ) ) -RRB- 10_1101-2021_01_07_425723 789 7 . . . 10_1101-2021_01_07_425723 790 1 856 856 CD 10_1101-2021_01_07_425723 790 2 59 59 CD 10_1101-2021_01_07_425723 790 3 . . . 10_1101-2021_01_07_425723 791 1 Bracher Bracher NNP 10_1101-2021_01_07_425723 791 2 , , , 10_1101-2021_01_07_425723 791 3 J. J. NNP 10_1101-2021_01_07_425723 791 4 M. M. NNP 10_1101-2021_01_07_425723 791 5 et et NNP 10_1101-2021_01_07_425723 791 6 al al NNP 10_1101-2021_01_07_425723 791 7 . . . 10_1101-2021_01_07_425723 792 1 Laboratory laboratory NN 10_1101-2021_01_07_425723 792 2 evolution evolution NN 10_1101-2021_01_07_425723 792 3 of of IN 10_1101-2021_01_07_425723 792 4 a a DT 10_1101-2021_01_07_425723 792 5 biotin biotin NN 10_1101-2021_01_07_425723 792 6 - - HYPH 10_1101-2021_01_07_425723 792 7 requiring require VBG 10_1101-2021_01_07_425723 792 8 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 792 9 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 792 10 strain strain NN 10_1101-2021_01_07_425723 792 11 for for IN 10_1101-2021_01_07_425723 792 12 857 857 CD 10_1101-2021_01_07_425723 792 13 full full JJ 10_1101-2021_01_07_425723 792 14 biotin biotin JJ 10_1101-2021_01_07_425723 792 15 prototrophy prototrophy NN 10_1101-2021_01_07_425723 792 16 and and CC 10_1101-2021_01_07_425723 792 17 identification identification NN 10_1101-2021_01_07_425723 792 18 of of IN 10_1101-2021_01_07_425723 792 19 causal causal JJ 10_1101-2021_01_07_425723 792 20 mutations mutation NNS 10_1101-2021_01_07_425723 792 21 . . . 10_1101-2021_01_07_425723 793 1 Appl Appl NNP 10_1101-2021_01_07_425723 793 2 . . . 10_1101-2021_01_07_425723 794 1 Environ Environ NNP 10_1101-2021_01_07_425723 794 2 . . . 10_1101-2021_01_07_425723 795 1 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 795 2 . . . 10_1101-2021_01_07_425723 796 1 83 83 CD 10_1101-2021_01_07_425723 796 2 , , , 10_1101-2021_01_07_425723 796 3 1–16 1–16 CD 10_1101-2021_01_07_425723 796 4 858 858 CD 10_1101-2021_01_07_425723 796 5 ( ( -LRB- 10_1101-2021_01_07_425723 796 6 2017 2017 CD 10_1101-2021_01_07_425723 796 7 ) ) -RRB- 10_1101-2021_01_07_425723 796 8 . . . 10_1101-2021_01_07_425723 797 1 859 859 CD 10_1101-2021_01_07_425723 797 2 60 60 CD 10_1101-2021_01_07_425723 797 3 . . . 10_1101-2021_01_07_425723 798 1 Lee Lee NNP 10_1101-2021_01_07_425723 798 2 , , , 10_1101-2021_01_07_425723 798 3 M. M. NNP 10_1101-2021_01_07_425723 798 4 E. E. NNP 10_1101-2021_01_07_425723 798 5 , , , 10_1101-2021_01_07_425723 798 6 DeLoache DeLoache NNP 10_1101-2021_01_07_425723 798 7 , , , 10_1101-2021_01_07_425723 798 8 W. W. NNP 10_1101-2021_01_07_425723 798 9 C. C. NNP 10_1101-2021_01_07_425723 798 10 , , , 10_1101-2021_01_07_425723 798 11 Cervantes Cervantes NNP 10_1101-2021_01_07_425723 798 12 , , , 10_1101-2021_01_07_425723 798 13 B. B. NNP 10_1101-2021_01_07_425723 799 1 & & CC 10_1101-2021_01_07_425723 799 2 Dueber Dueber NNP 10_1101-2021_01_07_425723 799 3 , , , 10_1101-2021_01_07_425723 799 4 J. J. NNP 10_1101-2021_01_07_425723 799 5 E. E. NNP 10_1101-2021_01_07_425723 799 6 A A NNP 10_1101-2021_01_07_425723 799 7 Highly highly RB 10_1101-2021_01_07_425723 799 8 Characterized characterize VBN 10_1101-2021_01_07_425723 799 9 Yeast yeast NN 10_1101-2021_01_07_425723 799 10 Toolkit Toolkit VBZ 10_1101-2021_01_07_425723 799 11 for for IN 10_1101-2021_01_07_425723 799 12 860 860 CD 10_1101-2021_01_07_425723 799 13 Modular Modular NNP 10_1101-2021_01_07_425723 799 14 , , , 10_1101-2021_01_07_425723 799 15 Multipart Multipart NNP 10_1101-2021_01_07_425723 799 16 Assembly Assembly NNP 10_1101-2021_01_07_425723 799 17 . . . 10_1101-2021_01_07_425723 800 1 ACS ACS NNP 10_1101-2021_01_07_425723 800 2 Synth Synth NNP 10_1101-2021_01_07_425723 800 3 . . . 10_1101-2021_01_07_425723 801 1 Biol Biol NNP 10_1101-2021_01_07_425723 801 2 . . . 10_1101-2021_01_07_425723 802 1 4 4 LS 10_1101-2021_01_07_425723 802 2 , , , 10_1101-2021_01_07_425723 802 3 975–986 975–986 CD 10_1101-2021_01_07_425723 802 4 ( ( -LRB- 10_1101-2021_01_07_425723 802 5 2015 2015 CD 10_1101-2021_01_07_425723 802 6 ) ) -RRB- 10_1101-2021_01_07_425723 802 7 . . . 10_1101-2021_01_07_425723 803 1 861 861 CD 10_1101-2021_01_07_425723 803 2 61 61 CD 10_1101-2021_01_07_425723 803 3 . . . 10_1101-2021_01_07_425723 804 1 Mans Mans NNP 10_1101-2021_01_07_425723 804 2 , , , 10_1101-2021_01_07_425723 804 3 R. R. NNP 10_1101-2021_01_07_425723 804 4 et et NNP 10_1101-2021_01_07_425723 804 5 al al NNP 10_1101-2021_01_07_425723 804 6 . . . 10_1101-2021_01_07_425723 805 1 CRISPR CRISPR NNP 10_1101-2021_01_07_425723 805 2 / / SYM 10_1101-2021_01_07_425723 805 3 Cas9 Cas9 NNP 10_1101-2021_01_07_425723 805 4 : : : 10_1101-2021_01_07_425723 805 5 A a DT 10_1101-2021_01_07_425723 805 6 molecular molecular JJ 10_1101-2021_01_07_425723 805 7 Swiss swiss JJ 10_1101-2021_01_07_425723 805 8 army army NN 10_1101-2021_01_07_425723 805 9 knife knife NN 10_1101-2021_01_07_425723 805 10 for for IN 10_1101-2021_01_07_425723 805 11 simultaneous simultaneous JJ 10_1101-2021_01_07_425723 805 12 introduction introduction NN 10_1101-2021_01_07_425723 805 13 of of IN 10_1101-2021_01_07_425723 805 14 862 862 CD 10_1101-2021_01_07_425723 805 15 multiple multiple JJ 10_1101-2021_01_07_425723 805 16 genetic genetic JJ 10_1101-2021_01_07_425723 805 17 modifications modification NNS 10_1101-2021_01_07_425723 805 18 in in IN 10_1101-2021_01_07_425723 805 19 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 805 20 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 805 21 . . . 10_1101-2021_01_07_425723 806 1 FEMS FEMS NNP 10_1101-2021_01_07_425723 806 2 Yeast Yeast NNP 10_1101-2021_01_07_425723 806 3 Res res NN 10_1101-2021_01_07_425723 806 4 . . . 10_1101-2021_01_07_425723 807 1 15 15 CD 10_1101-2021_01_07_425723 807 2 , , , 10_1101-2021_01_07_425723 807 3 1–15 1–15 CD 10_1101-2021_01_07_425723 807 4 ( ( -LRB- 10_1101-2021_01_07_425723 807 5 2015 2015 CD 10_1101-2021_01_07_425723 807 6 ) ) -RRB- 10_1101-2021_01_07_425723 807 7 . . . 10_1101-2021_01_07_425723 808 1 863 863 CD 10_1101-2021_01_07_425723 808 2 62 62 CD 10_1101-2021_01_07_425723 808 3 . . . 10_1101-2021_01_07_425723 809 1 Lorenz Lorenz NNP 10_1101-2021_01_07_425723 809 2 , , , 10_1101-2021_01_07_425723 809 3 R. R. NNP 10_1101-2021_01_07_425723 809 4 et et NNP 10_1101-2021_01_07_425723 809 5 al al NNP 10_1101-2021_01_07_425723 809 6 . . . 10_1101-2021_01_07_425723 810 1 ViennaRNA ViennaRNA NFP 10_1101-2021_01_07_425723 810 2 Package package NN 10_1101-2021_01_07_425723 810 3 2.0 2.0 CD 10_1101-2021_01_07_425723 810 4 . . . 10_1101-2021_01_07_425723 811 1 Algorithms Algorithms NNP 10_1101-2021_01_07_425723 811 2 Mol Mol NNP 10_1101-2021_01_07_425723 811 3 . . . 10_1101-2021_01_07_425723 812 1 Biol Biol NNP 10_1101-2021_01_07_425723 812 2 . . . 10_1101-2021_01_07_425723 813 1 6 6 CD 10_1101-2021_01_07_425723 813 2 , , , 10_1101-2021_01_07_425723 813 3 26 26 CD 10_1101-2021_01_07_425723 813 4 ( ( -LRB- 10_1101-2021_01_07_425723 813 5 2011 2011 CD 10_1101-2021_01_07_425723 813 6 ) ) -RRB- 10_1101-2021_01_07_425723 813 7 . . . 10_1101-2021_01_07_425723 814 1 864 864 CD 10_1101-2021_01_07_425723 814 2 63 63 CD 10_1101-2021_01_07_425723 814 3 . . . 10_1101-2021_01_07_425723 815 1 Hassing Hassing NNP 10_1101-2021_01_07_425723 815 2 , , , 10_1101-2021_01_07_425723 815 3 E. E. NNP 10_1101-2021_01_07_425723 815 4 J. J. NNP 10_1101-2021_01_07_425723 815 5 , , , 10_1101-2021_01_07_425723 815 6 de de NNP 10_1101-2021_01_07_425723 815 7 Groot Groot NNP 10_1101-2021_01_07_425723 815 8 , , , 10_1101-2021_01_07_425723 815 9 P. P. NNP 10_1101-2021_01_07_425723 815 10 A. A. NNP 10_1101-2021_01_07_425723 815 11 , , , 10_1101-2021_01_07_425723 815 12 Marquenie Marquenie NNP 10_1101-2021_01_07_425723 815 13 , , , 10_1101-2021_01_07_425723 815 14 V. V. NNP 10_1101-2021_01_07_425723 815 15 R. R. NNP 10_1101-2021_01_07_425723 815 16 , , , 10_1101-2021_01_07_425723 815 17 Pronk Pronk NNP 10_1101-2021_01_07_425723 815 18 , , , 10_1101-2021_01_07_425723 815 19 J. J. NNP 10_1101-2021_01_07_425723 815 20 T. T. NNP 10_1101-2021_01_07_425723 815 21 & & CC 10_1101-2021_01_07_425723 815 22 Daran Daran NNP 10_1101-2021_01_07_425723 815 23 , , , 10_1101-2021_01_07_425723 815 24 J. J. NNP 10_1101-2021_01_07_425723 815 25 M. M. NNP 10_1101-2021_01_07_425723 815 26 G. G. NNP 10_1101-2021_01_07_425723 815 27 Connecting Connecting NNP 10_1101-2021_01_07_425723 815 28 central central JJ 10_1101-2021_01_07_425723 815 29 865 865 CD 10_1101-2021_01_07_425723 815 30 carbon carbon NN 10_1101-2021_01_07_425723 815 31 and and CC 10_1101-2021_01_07_425723 815 32 aromatic aromatic JJ 10_1101-2021_01_07_425723 815 33 amino amino NN 10_1101-2021_01_07_425723 815 34 acid acid NN 10_1101-2021_01_07_425723 815 35 metabolisms metabolisms NNP 10_1101-2021_01_07_425723 815 36 to to TO 10_1101-2021_01_07_425723 815 37 improve improve VB 10_1101-2021_01_07_425723 815 38 de de FW 10_1101-2021_01_07_425723 815 39 novo novo NNP 10_1101-2021_01_07_425723 815 40 2-phenylethanol 2-phenylethanol CD 10_1101-2021_01_07_425723 815 41 production production NN 10_1101-2021_01_07_425723 815 42 in in IN 10_1101-2021_01_07_425723 815 43 866 866 CD 10_1101-2021_01_07_425723 815 44 Saccharomyces Saccharomyces NNPS 10_1101-2021_01_07_425723 815 45 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 815 46 . . . 10_1101-2021_01_07_425723 816 1 Metab Metab NNP 10_1101-2021_01_07_425723 816 2 . . . 10_1101-2021_01_07_425723 817 1 Eng Eng NNP 10_1101-2021_01_07_425723 817 2 . . . 10_1101-2021_01_07_425723 818 1 56 56 CD 10_1101-2021_01_07_425723 818 2 , , , 10_1101-2021_01_07_425723 818 3 165–180 165–180 CD 10_1101-2021_01_07_425723 818 4 ( ( -LRB- 10_1101-2021_01_07_425723 818 5 2019 2019 CD 10_1101-2021_01_07_425723 818 6 ) ) -RRB- 10_1101-2021_01_07_425723 818 7 . . . 10_1101-2021_01_07_425723 819 1 867 867 CD 10_1101-2021_01_07_425723 819 2 64 64 CD 10_1101-2021_01_07_425723 819 3 . . . 10_1101-2021_01_07_425723 820 1 Gietz Gietz NNP 10_1101-2021_01_07_425723 820 2 , , , 10_1101-2021_01_07_425723 820 3 R. R. NNP 10_1101-2021_01_07_425723 820 4 D. D. NNP 10_1101-2021_01_07_425723 820 5 & & CC 10_1101-2021_01_07_425723 820 6 Woods Woods NNP 10_1101-2021_01_07_425723 820 7 , , , 10_1101-2021_01_07_425723 820 8 R. R. NNP 10_1101-2021_01_07_425723 820 9 A. A. NNP 10_1101-2021_01_07_425723 821 1 Genetic genetic JJ 10_1101-2021_01_07_425723 821 2 Transformation Transformation NNP 10_1101-2021_01_07_425723 821 3 of of IN 10_1101-2021_01_07_425723 821 4 Yeast Yeast NNP 10_1101-2021_01_07_425723 821 5 . . . 10_1101-2021_01_07_425723 822 1 Biotechniques biotechnique NNS 10_1101-2021_01_07_425723 822 2 30 30 CD 10_1101-2021_01_07_425723 822 3 , , , 10_1101-2021_01_07_425723 822 4 816–831 816–831 CD 10_1101-2021_01_07_425723 822 5 ( ( -LRB- 10_1101-2021_01_07_425723 822 6 2001 2001 CD 10_1101-2021_01_07_425723 822 7 ) ) -RRB- 10_1101-2021_01_07_425723 822 8 . . . 10_1101-2021_01_07_425723 823 1 868 868 CD 10_1101-2021_01_07_425723 823 2 65 65 CD 10_1101-2021_01_07_425723 823 3 . . . 10_1101-2021_01_07_425723 824 1 Solis Solis NNP 10_1101-2021_01_07_425723 824 2 - - HYPH 10_1101-2021_01_07_425723 824 3 Escalante Escalante NNP 10_1101-2021_01_07_425723 824 4 , , , 10_1101-2021_01_07_425723 824 5 D. D. NNP 10_1101-2021_01_07_425723 824 6 et et NNP 10_1101-2021_01_07_425723 824 7 al al NNP 10_1101-2021_01_07_425723 824 8 . . . 10_1101-2021_01_07_425723 825 1 amdSYM amdSYM NNP 10_1101-2021_01_07_425723 825 2 , , , 10_1101-2021_01_07_425723 825 3 A a DT 10_1101-2021_01_07_425723 825 4 new new JJ 10_1101-2021_01_07_425723 825 5 dominant dominant JJ 10_1101-2021_01_07_425723 825 6 recyclable recyclable JJ 10_1101-2021_01_07_425723 825 7 marker marker NN 10_1101-2021_01_07_425723 825 8 cassette cassette NN 10_1101-2021_01_07_425723 825 9 for for IN 10_1101-2021_01_07_425723 825 10 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 825 11 869 869 CD 10_1101-2021_01_07_425723 825 12 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 825 13 . . . 10_1101-2021_01_07_425723 826 1 FEMS FEMS NNP 10_1101-2021_01_07_425723 826 2 Yeast Yeast NNP 10_1101-2021_01_07_425723 826 3 Res res NN 10_1101-2021_01_07_425723 826 4 . . . 10_1101-2021_01_07_425723 827 1 13 13 CD 10_1101-2021_01_07_425723 827 2 , , , 10_1101-2021_01_07_425723 827 3 126–139 126–139 CD 10_1101-2021_01_07_425723 827 4 ( ( -LRB- 10_1101-2021_01_07_425723 827 5 2013 2013 CD 10_1101-2021_01_07_425723 827 6 ) ) -RRB- 10_1101-2021_01_07_425723 827 7 . . . 10_1101-2021_01_07_425723 828 1 870 870 CD 10_1101-2021_01_07_425723 828 2 66 66 CD 10_1101-2021_01_07_425723 828 3 . . . 10_1101-2021_01_07_425723 829 1 Postma Postma NNP 10_1101-2021_01_07_425723 829 2 , , , 10_1101-2021_01_07_425723 829 3 E. E. NNP 10_1101-2021_01_07_425723 829 4 , , , 10_1101-2021_01_07_425723 829 5 Verduyn Verduyn NNP 10_1101-2021_01_07_425723 829 6 , , , 10_1101-2021_01_07_425723 829 7 C. C. NNP 10_1101-2021_01_07_425723 829 8 , , , 10_1101-2021_01_07_425723 829 9 Scheffers Scheffers NNP 10_1101-2021_01_07_425723 829 10 , , , 10_1101-2021_01_07_425723 829 11 W. W. NNP 10_1101-2021_01_07_425723 829 12 A. A. NNP 10_1101-2021_01_07_425723 830 1 & & CC 10_1101-2021_01_07_425723 830 2 Van Van NNP 10_1101-2021_01_07_425723 830 3 Dijken Dijken NNP 10_1101-2021_01_07_425723 830 4 , , , 10_1101-2021_01_07_425723 830 5 J. J. NNP 10_1101-2021_01_07_425723 830 6 P. P. NNP 10_1101-2021_01_07_425723 830 7 Enzymic Enzymic NNP 10_1101-2021_01_07_425723 830 8 analysis analysis NN 10_1101-2021_01_07_425723 830 9 of of IN 10_1101-2021_01_07_425723 830 10 the the DT 10_1101-2021_01_07_425723 830 11 crabtree crabtree NN 10_1101-2021_01_07_425723 830 12 871 871 CD 10_1101-2021_01_07_425723 830 13 effect effect NN 10_1101-2021_01_07_425723 830 14 in in IN 10_1101-2021_01_07_425723 830 15 glucose glucose NN 10_1101-2021_01_07_425723 830 16 - - HYPH 10_1101-2021_01_07_425723 830 17 limited limit VBN 10_1101-2021_01_07_425723 830 18 chemostat chemostat JJ 10_1101-2021_01_07_425723 830 19 cultures culture NNS 10_1101-2021_01_07_425723 830 20 of of IN 10_1101-2021_01_07_425723 830 21 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 830 22 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 830 23 . . . 10_1101-2021_01_07_425723 831 1 Appl Appl NNP 10_1101-2021_01_07_425723 831 2 . . . 10_1101-2021_01_07_425723 832 1 Environ Environ NNP 10_1101-2021_01_07_425723 832 2 . . . 10_1101-2021_01_07_425723 833 1 872 872 CD 10_1101-2021_01_07_425723 833 2 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 833 3 . . . 10_1101-2021_01_07_425723 834 1 55 55 CD 10_1101-2021_01_07_425723 834 2 , , , 10_1101-2021_01_07_425723 834 3 468–477 468–477 CD 10_1101-2021_01_07_425723 834 4 ( ( -LRB- 10_1101-2021_01_07_425723 834 5 1989 1989 CD 10_1101-2021_01_07_425723 834 6 ) ) -RRB- 10_1101-2021_01_07_425723 834 7 . . . 10_1101-2021_01_07_425723 835 1 873 873 CD 10_1101-2021_01_07_425723 835 2 67 67 CD 10_1101-2021_01_07_425723 835 3 . . . 10_1101-2021_01_07_425723 836 1 Mashego Mashego NNP 10_1101-2021_01_07_425723 836 2 , , , 10_1101-2021_01_07_425723 836 3 M. M. NNP 10_1101-2021_01_07_425723 836 4 R. R. NNP 10_1101-2021_01_07_425723 836 5 , , , 10_1101-2021_01_07_425723 836 6 van van NNP 10_1101-2021_01_07_425723 836 7 Gulik Gulik NNP 10_1101-2021_01_07_425723 836 8 , , , 10_1101-2021_01_07_425723 836 9 W. W. NNP 10_1101-2021_01_07_425723 836 10 M. M. NNP 10_1101-2021_01_07_425723 836 11 , , , 10_1101-2021_01_07_425723 836 12 Vinke Vinke NNP 10_1101-2021_01_07_425723 836 13 , , , 10_1101-2021_01_07_425723 836 14 J. J. NNP 10_1101-2021_01_07_425723 836 15 L. L. NNP 10_1101-2021_01_07_425723 836 16 & & CC 10_1101-2021_01_07_425723 836 17 Heijnen Heijnen NNP 10_1101-2021_01_07_425723 836 18 , , , 10_1101-2021_01_07_425723 836 19 J. J. NNP 10_1101-2021_01_07_425723 836 20 J. J. NNP 10_1101-2021_01_07_425723 837 1 Critical critical JJ 10_1101-2021_01_07_425723 837 2 evaluation evaluation NN 10_1101-2021_01_07_425723 837 3 of of IN 10_1101-2021_01_07_425723 837 4 sampling sample VBG 10_1101-2021_01_07_425723 837 5 874 874 CD 10_1101-2021_01_07_425723 837 6 techniques technique NNS 10_1101-2021_01_07_425723 837 7 for for IN 10_1101-2021_01_07_425723 837 8 residual residual JJ 10_1101-2021_01_07_425723 837 9 glucose glucose NN 10_1101-2021_01_07_425723 837 10 determination determination NN 10_1101-2021_01_07_425723 837 11 in in IN 10_1101-2021_01_07_425723 837 12 carbon carbon NN 10_1101-2021_01_07_425723 837 13 - - HYPH 10_1101-2021_01_07_425723 837 14 limited limit VBN 10_1101-2021_01_07_425723 837 15 chemostat chemostat JJ 10_1101-2021_01_07_425723 837 16 culture culture NN 10_1101-2021_01_07_425723 837 17 875 875 CD 10_1101-2021_01_07_425723 837 18 .CC .CC NFP 10_1101-2021_01_07_425723 837 19 - - HYPH 10_1101-2021_01_07_425723 837 20 BY by IN 10_1101-2021_01_07_425723 837 21 - - HYPH 10_1101-2021_01_07_425723 837 22 NC NC NNP 10_1101-2021_01_07_425723 837 23 - - HYPH 10_1101-2021_01_07_425723 837 24 ND ND NNP 10_1101-2021_01_07_425723 837 25 4.0 4.0 CD 10_1101-2021_01_07_425723 837 26 International International NNP 10_1101-2021_01_07_425723 837 27 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 837 28 under under IN 10_1101-2021_01_07_425723 837 29 a a DT 10_1101-2021_01_07_425723 837 30 ( ( -LRB- 10_1101-2021_01_07_425723 837 31 which which WDT 10_1101-2021_01_07_425723 837 32 was be VBD 10_1101-2021_01_07_425723 837 33 not not RB 10_1101-2021_01_07_425723 837 34 certified certify VBN 10_1101-2021_01_07_425723 837 35 by by IN 10_1101-2021_01_07_425723 837 36 peer peer NN 10_1101-2021_01_07_425723 837 37 review review NN 10_1101-2021_01_07_425723 837 38 ) ) -RRB- 10_1101-2021_01_07_425723 837 39 is be VBZ 10_1101-2021_01_07_425723 837 40 the the DT 10_1101-2021_01_07_425723 837 41 author author NN 10_1101-2021_01_07_425723 837 42 / / SYM 10_1101-2021_01_07_425723 837 43 funder funder NN 10_1101-2021_01_07_425723 837 44 , , , 10_1101-2021_01_07_425723 837 45 who who WP 10_1101-2021_01_07_425723 837 46 has have VBZ 10_1101-2021_01_07_425723 837 47 granted grant VBN 10_1101-2021_01_07_425723 837 48 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 837 49 a a DT 10_1101-2021_01_07_425723 837 50 license license NN 10_1101-2021_01_07_425723 837 51 to to TO 10_1101-2021_01_07_425723 837 52 display display VB 10_1101-2021_01_07_425723 837 53 the the DT 10_1101-2021_01_07_425723 837 54 preprint preprint NN 10_1101-2021_01_07_425723 837 55 in in IN 10_1101-2021_01_07_425723 837 56 perpetuity perpetuity NN 10_1101-2021_01_07_425723 837 57 . . . 10_1101-2021_01_07_425723 838 1 It -PRON- PRP 10_1101-2021_01_07_425723 838 2 is be VBZ 10_1101-2021_01_07_425723 838 3 made make VBN 10_1101-2021_01_07_425723 838 4 The the DT 10_1101-2021_01_07_425723 838 5 copyright copyright NN 10_1101-2021_01_07_425723 838 6 holder holder NN 10_1101-2021_01_07_425723 838 7 for for IN 10_1101-2021_01_07_425723 838 8 this this DT 10_1101-2021_01_07_425723 838 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 838 10 version version NN 10_1101-2021_01_07_425723 838 11 posted post VBD 10_1101-2021_01_07_425723 838 12 January January NNP 10_1101-2021_01_07_425723 838 13 8 8 CD 10_1101-2021_01_07_425723 838 14 , , , 10_1101-2021_01_07_425723 838 15 2021 2021 CD 10_1101-2021_01_07_425723 838 16 . . . 10_1101-2021_01_07_425723 838 17 ; ; : 10_1101-2021_01_07_425723 838 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 838 19 : : : 10_1101-2021_01_07_425723 838 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 838 21 preprint preprint NN 10_1101-2021_01_07_425723 838 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 838 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 838 24 44 44 CD 10_1101-2021_01_07_425723 838 25 ofSaccharomyces ofsaccharomyces NN 10_1101-2021_01_07_425723 838 26 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 838 27 . . . 10_1101-2021_01_07_425723 839 1 Biotechnol Biotechnol NNS 10_1101-2021_01_07_425723 839 2 . . . 10_1101-2021_01_07_425723 840 1 Bioeng Bioeng NNP 10_1101-2021_01_07_425723 840 2 . . . 10_1101-2021_01_07_425723 841 1 83 83 CD 10_1101-2021_01_07_425723 841 2 , , , 10_1101-2021_01_07_425723 841 3 395–399 395–399 CD 10_1101-2021_01_07_425723 841 4 ( ( -LRB- 10_1101-2021_01_07_425723 841 5 2003 2003 CD 10_1101-2021_01_07_425723 841 6 ) ) -RRB- 10_1101-2021_01_07_425723 841 7 . . . 10_1101-2021_01_07_425723 842 1 876 876 CD 10_1101-2021_01_07_425723 842 2 68 68 CD 10_1101-2021_01_07_425723 842 3 . . . 10_1101-2021_01_07_425723 843 1 Boender boender NN 10_1101-2021_01_07_425723 843 2 , , , 10_1101-2021_01_07_425723 843 3 L. L. NNP 10_1101-2021_01_07_425723 843 4 G. G. NNP 10_1101-2021_01_07_425723 843 5 M. M. NNP 10_1101-2021_01_07_425723 843 6 , , , 10_1101-2021_01_07_425723 843 7 De De NNP 10_1101-2021_01_07_425723 843 8 Hulster Hulster NNP 10_1101-2021_01_07_425723 843 9 , , , 10_1101-2021_01_07_425723 843 10 E. E. NNP 10_1101-2021_01_07_425723 843 11 A. a. NN 10_1101-2021_01_07_425723 843 12 F. F. NNP 10_1101-2021_01_07_425723 843 13 , , , 10_1101-2021_01_07_425723 843 14 Van Van NNP 10_1101-2021_01_07_425723 843 15 Maris Maris NNP 10_1101-2021_01_07_425723 843 16 , , , 10_1101-2021_01_07_425723 843 17 A. a. NN 10_1101-2021_01_07_425723 843 18 J. J. NNP 10_1101-2021_01_07_425723 844 1 A. A. NNP 10_1101-2021_01_07_425723 844 2 , , , 10_1101-2021_01_07_425723 844 3 Daran Daran NNP 10_1101-2021_01_07_425723 844 4 - - HYPH 10_1101-2021_01_07_425723 844 5 Lapujade Lapujade NNP 10_1101-2021_01_07_425723 844 6 , , , 10_1101-2021_01_07_425723 844 7 P. P. NNP 10_1101-2021_01_07_425723 844 8 A. A. NNP 10_1101-2021_01_07_425723 844 9 S. S. NNP 10_1101-2021_01_07_425723 844 10 & & CC 10_1101-2021_01_07_425723 844 11 Pronk Pronk NNP 10_1101-2021_01_07_425723 844 12 , , , 10_1101-2021_01_07_425723 844 13 J. J. NNP 10_1101-2021_01_07_425723 844 14 T. T. NNP 10_1101-2021_01_07_425723 844 15 877 877 CD 10_1101-2021_01_07_425723 844 16 Quantitative quantitative JJ 10_1101-2021_01_07_425723 844 17 physiology physiology NN 10_1101-2021_01_07_425723 844 18 of of IN 10_1101-2021_01_07_425723 844 19 Saccharomyces saccharomyce NNS 10_1101-2021_01_07_425723 844 20 cerevisiae cerevisiae VBZ 10_1101-2021_01_07_425723 844 21 at at IN 10_1101-2021_01_07_425723 844 22 near near JJ 10_1101-2021_01_07_425723 844 23 - - HYPH 10_1101-2021_01_07_425723 844 24 zero zero CD 10_1101-2021_01_07_425723 844 25 specific specific JJ 10_1101-2021_01_07_425723 844 26 growth growth NN 10_1101-2021_01_07_425723 844 27 rates rate NNS 10_1101-2021_01_07_425723 844 28 . . . 10_1101-2021_01_07_425723 845 1 Appl Appl NNP 10_1101-2021_01_07_425723 845 2 . . . 10_1101-2021_01_07_425723 846 1 878 878 CD 10_1101-2021_01_07_425723 846 2 Environ Environ NNP 10_1101-2021_01_07_425723 846 3 . . . 10_1101-2021_01_07_425723 847 1 Microbiol Microbiol NNP 10_1101-2021_01_07_425723 847 2 . . . 10_1101-2021_01_07_425723 848 1 75 75 CD 10_1101-2021_01_07_425723 848 2 , , , 10_1101-2021_01_07_425723 848 3 5607–5614 5607–5614 CD 10_1101-2021_01_07_425723 848 4 ( ( -LRB- 10_1101-2021_01_07_425723 848 5 2009 2009 CD 10_1101-2021_01_07_425723 848 6 ) ) -RRB- 10_1101-2021_01_07_425723 848 7 . . . 10_1101-2021_01_07_425723 849 1 879 879 CD 10_1101-2021_01_07_425723 849 2 69 69 CD 10_1101-2021_01_07_425723 849 3 . . . 10_1101-2021_01_07_425723 850 1 Koren Koren NNP 10_1101-2021_01_07_425723 850 2 , , , 10_1101-2021_01_07_425723 850 3 S. S. NNP 10_1101-2021_01_07_425723 850 4 et et NNP 10_1101-2021_01_07_425723 850 5 al al NNP 10_1101-2021_01_07_425723 850 6 . . . 10_1101-2021_01_07_425723 851 1 Canu Canu NNP 10_1101-2021_01_07_425723 851 2 : : : 10_1101-2021_01_07_425723 851 3 Scalable scalable JJ 10_1101-2021_01_07_425723 851 4 and and CC 10_1101-2021_01_07_425723 851 5 accurate accurate JJ 10_1101-2021_01_07_425723 851 6 long long RB 10_1101-2021_01_07_425723 851 7 - - HYPH 10_1101-2021_01_07_425723 851 8 read read VBN 10_1101-2021_01_07_425723 851 9 assembly assembly NN 10_1101-2021_01_07_425723 851 10 via via IN 10_1101-2021_01_07_425723 851 11 adaptive adaptive JJ 10_1101-2021_01_07_425723 851 12 κ κ NNP 10_1101-2021_01_07_425723 851 13 - - HYPH 10_1101-2021_01_07_425723 851 14 mer mer NN 10_1101-2021_01_07_425723 851 15 weighting weighting NN 10_1101-2021_01_07_425723 851 16 and and CC 10_1101-2021_01_07_425723 851 17 880 880 CD 10_1101-2021_01_07_425723 851 18 repeat repeat NN 10_1101-2021_01_07_425723 851 19 separation separation NN 10_1101-2021_01_07_425723 851 20 . . . 10_1101-2021_01_07_425723 852 1 Genome Genome NNP 10_1101-2021_01_07_425723 852 2 Res Res NNP 10_1101-2021_01_07_425723 852 3 . . . 10_1101-2021_01_07_425723 853 1 27 27 CD 10_1101-2021_01_07_425723 853 2 , , , 10_1101-2021_01_07_425723 853 3 722–736 722–736 CD 10_1101-2021_01_07_425723 853 4 ( ( -LRB- 10_1101-2021_01_07_425723 853 5 2017 2017 CD 10_1101-2021_01_07_425723 853 6 ) ) -RRB- 10_1101-2021_01_07_425723 853 7 . . . 10_1101-2021_01_07_425723 854 1 881 881 CD 10_1101-2021_01_07_425723 854 2 70 70 CD 10_1101-2021_01_07_425723 854 3 . . . 10_1101-2021_01_07_425723 855 1 Kolmogorov Kolmogorov NNP 10_1101-2021_01_07_425723 855 2 , , , 10_1101-2021_01_07_425723 855 3 M. M. NNP 10_1101-2021_01_07_425723 855 4 , , , 10_1101-2021_01_07_425723 855 5 Yuan Yuan NNP 10_1101-2021_01_07_425723 855 6 , , , 10_1101-2021_01_07_425723 855 7 J. J. NNP 10_1101-2021_01_07_425723 855 8 , , , 10_1101-2021_01_07_425723 855 9 Lin Lin NNP 10_1101-2021_01_07_425723 855 10 , , , 10_1101-2021_01_07_425723 855 11 Y. Y. NNP 10_1101-2021_01_07_425723 856 1 & & CC 10_1101-2021_01_07_425723 856 2 Pevzner Pevzner NNP 10_1101-2021_01_07_425723 856 3 , , , 10_1101-2021_01_07_425723 856 4 P. P. NNP 10_1101-2021_01_07_425723 856 5 A. A. NNP 10_1101-2021_01_07_425723 857 1 Assembly assembly NN 10_1101-2021_01_07_425723 857 2 of of IN 10_1101-2021_01_07_425723 857 3 long long JJ 10_1101-2021_01_07_425723 857 4 , , , 10_1101-2021_01_07_425723 857 5 error error NN 10_1101-2021_01_07_425723 857 6 - - HYPH 10_1101-2021_01_07_425723 857 7 prone prone JJ 10_1101-2021_01_07_425723 857 8 reads read VBZ 10_1101-2021_01_07_425723 857 9 using use VBG 10_1101-2021_01_07_425723 857 10 882 882 CD 10_1101-2021_01_07_425723 857 11 repeat repeat NN 10_1101-2021_01_07_425723 857 12 graphs graphs NN 10_1101-2021_01_07_425723 857 13 . . . 10_1101-2021_01_07_425723 858 1 Nat Nat NNP 10_1101-2021_01_07_425723 858 2 . . . 10_1101-2021_01_07_425723 859 1 Biotechnol Biotechnol NNS 10_1101-2021_01_07_425723 859 2 . . . 10_1101-2021_01_07_425723 860 1 37 37 CD 10_1101-2021_01_07_425723 860 2 , , , 10_1101-2021_01_07_425723 860 3 540–546 540–546 CD 10_1101-2021_01_07_425723 860 4 ( ( -LRB- 10_1101-2021_01_07_425723 860 5 2019 2019 CD 10_1101-2021_01_07_425723 860 6 ) ) -RRB- 10_1101-2021_01_07_425723 860 7 . . . 10_1101-2021_01_07_425723 861 1 883 883 CD 10_1101-2021_01_07_425723 861 2 71 71 CD 10_1101-2021_01_07_425723 861 3 . . . 10_1101-2021_01_07_425723 862 1 Walker Walker NNP 10_1101-2021_01_07_425723 862 2 , , , 10_1101-2021_01_07_425723 862 3 B. B. NNP 10_1101-2021_01_07_425723 862 4 J. J. NNP 10_1101-2021_01_07_425723 862 5 et et NNP 10_1101-2021_01_07_425723 862 6 al al NNP 10_1101-2021_01_07_425723 862 7 . . . 10_1101-2021_01_07_425723 863 1 Pilon pilon NN 10_1101-2021_01_07_425723 863 2     _SP 10_1101-2021_01_07_425723 863 3 : : : 10_1101-2021_01_07_425723 863 4 An an DT 10_1101-2021_01_07_425723 863 5 Integrated Integrated NNP 10_1101-2021_01_07_425723 863 6 Tool Tool NNP 10_1101-2021_01_07_425723 863 7 for for IN 10_1101-2021_01_07_425723 863 8 Comprehensive Comprehensive NNP 10_1101-2021_01_07_425723 863 9 Microbial Microbial NNP 10_1101-2021_01_07_425723 863 10 Variant Variant NNP 10_1101-2021_01_07_425723 863 11 Detection Detection NNP 10_1101-2021_01_07_425723 863 12 and and CC 10_1101-2021_01_07_425723 863 13 884 884 CD 10_1101-2021_01_07_425723 863 14 Genome Genome NNP 10_1101-2021_01_07_425723 863 15 Assembly Assembly NNP 10_1101-2021_01_07_425723 863 16 Improvement Improvement NNP 10_1101-2021_01_07_425723 863 17 . . . 10_1101-2021_01_07_425723 864 1 PLoS PLoS : 10_1101-2021_01_07_425723 864 2 One one CD 10_1101-2021_01_07_425723 864 3 9 9 CD 10_1101-2021_01_07_425723 864 4 , , , 10_1101-2021_01_07_425723 864 5 ( ( -LRB- 10_1101-2021_01_07_425723 864 6 2014 2014 CD 10_1101-2021_01_07_425723 864 7 ) ) -RRB- 10_1101-2021_01_07_425723 864 8 . . . 10_1101-2021_01_07_425723 865 1 885 885 CD 10_1101-2021_01_07_425723 865 2 72 72 CD 10_1101-2021_01_07_425723 865 3 . . . 10_1101-2021_01_07_425723 866 1 Palmer Palmer NNP 10_1101-2021_01_07_425723 866 2 , , , 10_1101-2021_01_07_425723 866 3 J. J. NNP 10_1101-2021_01_07_425723 867 1 & & CC 10_1101-2021_01_07_425723 867 2 Stajich Stajich NNP 10_1101-2021_01_07_425723 867 3 , , , 10_1101-2021_01_07_425723 867 4 J. J. NNP 10_1101-2021_01_07_425723 867 5 funannotate funannotate NN 10_1101-2021_01_07_425723 867 6 . . . 10_1101-2021_01_07_425723 868 1 ( ( -LRB- 10_1101-2021_01_07_425723 868 2 2019 2019 CD 10_1101-2021_01_07_425723 868 3 ) ) -RRB- 10_1101-2021_01_07_425723 868 4 . . . 10_1101-2021_01_07_425723 869 1 doi:10.5281 doi:10.5281 NNP 10_1101-2021_01_07_425723 869 2 / / SYM 10_1101-2021_01_07_425723 869 3 zenodo.3548120 zenodo.3548120 NNP 10_1101-2021_01_07_425723 869 4 886 886 CD 10_1101-2021_01_07_425723 869 5 73 73 CD 10_1101-2021_01_07_425723 869 6 . . . 10_1101-2021_01_07_425723 870 1 Jones Jones NNP 10_1101-2021_01_07_425723 870 2 , , , 10_1101-2021_01_07_425723 870 3 P. P. NNP 10_1101-2021_01_07_425723 870 4 et et NNP 10_1101-2021_01_07_425723 870 5 al al NNP 10_1101-2021_01_07_425723 870 6 . . . 10_1101-2021_01_07_425723 871 1 InterProScan InterProScan NNP 10_1101-2021_01_07_425723 871 2 5 5 CD 10_1101-2021_01_07_425723 871 3 : : : 10_1101-2021_01_07_425723 871 4 Genome genome NN 10_1101-2021_01_07_425723 871 5 - - HYPH 10_1101-2021_01_07_425723 871 6 scale scale NN 10_1101-2021_01_07_425723 871 7 protein protein NN 10_1101-2021_01_07_425723 871 8 function function NN 10_1101-2021_01_07_425723 871 9 classification classification NN 10_1101-2021_01_07_425723 871 10 . . . 10_1101-2021_01_07_425723 872 1 Bioinformatics Bioinformatics NNP 10_1101-2021_01_07_425723 872 2 30 30 CD 10_1101-2021_01_07_425723 872 3 , , , 10_1101-2021_01_07_425723 872 4 887 887 CD 10_1101-2021_01_07_425723 872 5 1236–1240 1236–1240 CD 10_1101-2021_01_07_425723 872 6 ( ( -LRB- 10_1101-2021_01_07_425723 872 7 2014 2014 CD 10_1101-2021_01_07_425723 872 8 ) ) -RRB- 10_1101-2021_01_07_425723 872 9 . . . 10_1101-2021_01_07_425723 873 1 888 888 CD 10_1101-2021_01_07_425723 873 2 74 74 CD 10_1101-2021_01_07_425723 873 3 . . . 10_1101-2021_01_07_425723 874 1 Bankevich Bankevich NNP 10_1101-2021_01_07_425723 874 2 , , , 10_1101-2021_01_07_425723 874 3 A. A. NNP 10_1101-2021_01_07_425723 874 4 et et FW 10_1101-2021_01_07_425723 874 5 al al NNP 10_1101-2021_01_07_425723 874 6 . . . 10_1101-2021_01_07_425723 875 1 SPAdes SPAdes NNP 10_1101-2021_01_07_425723 875 2 : : : 10_1101-2021_01_07_425723 875 3 A a DT 10_1101-2021_01_07_425723 875 4 new new JJ 10_1101-2021_01_07_425723 875 5 genome genome JJ 10_1101-2021_01_07_425723 875 6 assembly assembly NN 10_1101-2021_01_07_425723 875 7 algorithm algorithm NN 10_1101-2021_01_07_425723 875 8 and and CC 10_1101-2021_01_07_425723 875 9 its -PRON- PRP$ 10_1101-2021_01_07_425723 875 10 applications application NNS 10_1101-2021_01_07_425723 875 11 to to IN 10_1101-2021_01_07_425723 875 12 single single JJ 10_1101-2021_01_07_425723 875 13 - - HYPH 10_1101-2021_01_07_425723 875 14 cell cell NN 10_1101-2021_01_07_425723 875 15 889 889 CD 10_1101-2021_01_07_425723 875 16 sequencing sequencing NN 10_1101-2021_01_07_425723 875 17 . . . 10_1101-2021_01_07_425723 876 1 J. J. NNP 10_1101-2021_01_07_425723 876 2 Comput Comput NNP 10_1101-2021_01_07_425723 876 3 . . . 10_1101-2021_01_07_425723 877 1 Biol Biol NNP 10_1101-2021_01_07_425723 877 2 . . . 10_1101-2021_01_07_425723 878 1 19 19 CD 10_1101-2021_01_07_425723 878 2 , , , 10_1101-2021_01_07_425723 878 3 455–477 455–477 CD 10_1101-2021_01_07_425723 878 4 ( ( -LRB- 10_1101-2021_01_07_425723 878 5 2012 2012 CD 10_1101-2021_01_07_425723 878 6 ) ) -RRB- 10_1101-2021_01_07_425723 878 7 . . . 10_1101-2021_01_07_425723 879 1 890 890 CD 10_1101-2021_01_07_425723 879 2 75 75 CD 10_1101-2021_01_07_425723 879 3 . . . 10_1101-2021_01_07_425723 880 1 Grabherr Grabherr NNP 10_1101-2021_01_07_425723 880 2 , , , 10_1101-2021_01_07_425723 880 3 M. M. NNP 10_1101-2021_01_07_425723 880 4 G. G. NNP 10_1101-2021_01_07_425723 880 5 et et NNP 10_1101-2021_01_07_425723 880 6 al al NNP 10_1101-2021_01_07_425723 880 7 . . . 10_1101-2021_01_07_425723 881 1 Full full JJ 10_1101-2021_01_07_425723 881 2 - - HYPH 10_1101-2021_01_07_425723 881 3 length length NN 10_1101-2021_01_07_425723 881 4 transcriptome transcriptome DT 10_1101-2021_01_07_425723 881 5 assembly assembly NN 10_1101-2021_01_07_425723 881 6 from from IN 10_1101-2021_01_07_425723 881 7 RNA RNA NNP 10_1101-2021_01_07_425723 881 8 - - HYPH 10_1101-2021_01_07_425723 881 9 Seq Seq NNP 10_1101-2021_01_07_425723 881 10 data datum NNS 10_1101-2021_01_07_425723 881 11 without without IN 10_1101-2021_01_07_425723 881 12 a a DT 10_1101-2021_01_07_425723 881 13 reference reference NN 10_1101-2021_01_07_425723 881 14 891 891 CD 10_1101-2021_01_07_425723 881 15 genome genome NN 10_1101-2021_01_07_425723 881 16 . . . 10_1101-2021_01_07_425723 882 1 Nat Nat NNP 10_1101-2021_01_07_425723 882 2 . . . 10_1101-2021_01_07_425723 883 1 Biotechnol Biotechnol NNS 10_1101-2021_01_07_425723 883 2 . . . 10_1101-2021_01_07_425723 884 1 29 29 CD 10_1101-2021_01_07_425723 884 2 , , , 10_1101-2021_01_07_425723 884 3 644–652 644–652 CD 10_1101-2021_01_07_425723 884 4 ( ( -LRB- 10_1101-2021_01_07_425723 884 5 2011 2011 CD 10_1101-2021_01_07_425723 884 6 ) ) -RRB- 10_1101-2021_01_07_425723 884 7 . . . 10_1101-2021_01_07_425723 885 1 892 892 CD 10_1101-2021_01_07_425723 885 2 76 76 CD 10_1101-2021_01_07_425723 885 3 . . . 10_1101-2021_01_07_425723 886 1 Langmead Langmead NNP 10_1101-2021_01_07_425723 886 2 , , , 10_1101-2021_01_07_425723 886 3 B. B. NNP 10_1101-2021_01_07_425723 886 4 , , , 10_1101-2021_01_07_425723 886 5 Trapnell Trapnell NNP 10_1101-2021_01_07_425723 886 6 , , , 10_1101-2021_01_07_425723 886 7 C. C. NNP 10_1101-2021_01_07_425723 886 8 , , , 10_1101-2021_01_07_425723 886 9 Pop Pop NNP 10_1101-2021_01_07_425723 886 10 , , , 10_1101-2021_01_07_425723 886 11 M. M. NNP 10_1101-2021_01_07_425723 886 12 & & CC 10_1101-2021_01_07_425723 886 13 Salzberg Salzberg NNP 10_1101-2021_01_07_425723 886 14 , , , 10_1101-2021_01_07_425723 886 15 S. S. NNP 10_1101-2021_01_07_425723 886 16 L. L. NNP 10_1101-2021_01_07_425723 886 17 Ultrafast Ultrafast NNP 10_1101-2021_01_07_425723 886 18 and and CC 10_1101-2021_01_07_425723 886 19 memory memory NN 10_1101-2021_01_07_425723 886 20 - - HYPH 10_1101-2021_01_07_425723 886 21 efficient efficient JJ 10_1101-2021_01_07_425723 886 22 alignment alignment NN 10_1101-2021_01_07_425723 886 23 of of IN 10_1101-2021_01_07_425723 886 24 893 893 CD 10_1101-2021_01_07_425723 886 25 short short JJ 10_1101-2021_01_07_425723 886 26 DNA DNA NNP 10_1101-2021_01_07_425723 886 27 sequences sequence NNS 10_1101-2021_01_07_425723 886 28 to to IN 10_1101-2021_01_07_425723 886 29 the the DT 10_1101-2021_01_07_425723 886 30 human human JJ 10_1101-2021_01_07_425723 886 31 genome genome NN 10_1101-2021_01_07_425723 886 32 . . . 10_1101-2021_01_07_425723 887 1 Genome Genome NNP 10_1101-2021_01_07_425723 887 2 Biol Biol NNP 10_1101-2021_01_07_425723 887 3 . . . 10_1101-2021_01_07_425723 888 1 10 10 CD 10_1101-2021_01_07_425723 888 2 , , , 10_1101-2021_01_07_425723 888 3 R25 R25 NNP 10_1101-2021_01_07_425723 888 4 ( ( -LRB- 10_1101-2021_01_07_425723 888 5 2009 2009 CD 10_1101-2021_01_07_425723 888 6 ) ) -RRB- 10_1101-2021_01_07_425723 888 7 . . . 10_1101-2021_01_07_425723 889 1 894 894 CD 10_1101-2021_01_07_425723 889 2 77 77 CD 10_1101-2021_01_07_425723 889 3 . . . 10_1101-2021_01_07_425723 890 1 Li Li NNP 10_1101-2021_01_07_425723 890 2 , , , 10_1101-2021_01_07_425723 890 3 H. H. NNP 10_1101-2021_01_07_425723 890 4 et et NNP 10_1101-2021_01_07_425723 890 5 al al NNP 10_1101-2021_01_07_425723 890 6 . . . 10_1101-2021_01_07_425723 891 1 The the DT 10_1101-2021_01_07_425723 891 2 Sequence Sequence NNP 10_1101-2021_01_07_425723 891 3 Alignment Alignment NNP 10_1101-2021_01_07_425723 891 4 / / SYM 10_1101-2021_01_07_425723 891 5 Map Map NNP 10_1101-2021_01_07_425723 891 6 format format NN 10_1101-2021_01_07_425723 891 7 and and CC 10_1101-2021_01_07_425723 891 8 SAMtools samtool NNS 10_1101-2021_01_07_425723 891 9 . . . 10_1101-2021_01_07_425723 892 1 Bioinformatics Bioinformatics NNP 10_1101-2021_01_07_425723 892 2 25 25 CD 10_1101-2021_01_07_425723 892 3 , , , 10_1101-2021_01_07_425723 892 4 2078–2079 2078–2079 CD 10_1101-2021_01_07_425723 892 5 895 895 CD 10_1101-2021_01_07_425723 892 6 ( ( -LRB- 10_1101-2021_01_07_425723 892 7 2009 2009 CD 10_1101-2021_01_07_425723 892 8 ) ) -RRB- 10_1101-2021_01_07_425723 892 9 . . . 10_1101-2021_01_07_425723 893 1 896 896 CD 10_1101-2021_01_07_425723 893 2 78 78 CD 10_1101-2021_01_07_425723 893 3 . . . 10_1101-2021_01_07_425723 894 1 Liao Liao NNP 10_1101-2021_01_07_425723 894 2 , , , 10_1101-2021_01_07_425723 894 3 Y. Y. NNP 10_1101-2021_01_07_425723 894 4 , , , 10_1101-2021_01_07_425723 894 5 Smyth Smyth NNP 10_1101-2021_01_07_425723 894 6 , , , 10_1101-2021_01_07_425723 894 7 G. G. NNP 10_1101-2021_01_07_425723 894 8 K. K. NNP 10_1101-2021_01_07_425723 894 9 & & CC 10_1101-2021_01_07_425723 894 10 Shi Shi NNP 10_1101-2021_01_07_425723 894 11 , , , 10_1101-2021_01_07_425723 894 12 W. W. NNP 10_1101-2021_01_07_425723 894 13 FeatureCounts FeatureCounts NNP 10_1101-2021_01_07_425723 894 14 : : : 10_1101-2021_01_07_425723 894 15 An an DT 10_1101-2021_01_07_425723 894 16 efficient efficient JJ 10_1101-2021_01_07_425723 894 17 general general JJ 10_1101-2021_01_07_425723 894 18 purpose purpose NN 10_1101-2021_01_07_425723 894 19 program program NN 10_1101-2021_01_07_425723 894 20 for for IN 10_1101-2021_01_07_425723 894 21 assigning assign VBG 10_1101-2021_01_07_425723 894 22 897 897 CD 10_1101-2021_01_07_425723 894 23 sequence sequence NN 10_1101-2021_01_07_425723 894 24 reads read VBZ 10_1101-2021_01_07_425723 894 25 to to IN 10_1101-2021_01_07_425723 894 26 genomic genomic JJ 10_1101-2021_01_07_425723 894 27 features feature NNS 10_1101-2021_01_07_425723 894 28 . . . 10_1101-2021_01_07_425723 895 1 Bioinformatics Bioinformatics NNP 10_1101-2021_01_07_425723 895 2 30 30 CD 10_1101-2021_01_07_425723 895 3 , , , 10_1101-2021_01_07_425723 895 4 923–930 923–930 CD 10_1101-2021_01_07_425723 895 5 ( ( -LRB- 10_1101-2021_01_07_425723 895 6 2014 2014 CD 10_1101-2021_01_07_425723 895 7 ) ) -RRB- 10_1101-2021_01_07_425723 895 8 . . . 10_1101-2021_01_07_425723 896 1 898 898 CD 10_1101-2021_01_07_425723 896 2 79 79 CD 10_1101-2021_01_07_425723 896 3 . . . 10_1101-2021_01_07_425723 897 1 McCarthy McCarthy NNP 10_1101-2021_01_07_425723 897 2 , , , 10_1101-2021_01_07_425723 897 3 D. D. NNP 10_1101-2021_01_07_425723 897 4 J. J. NNP 10_1101-2021_01_07_425723 897 5 , , , 10_1101-2021_01_07_425723 897 6 Chen Chen NNP 10_1101-2021_01_07_425723 897 7 , , , 10_1101-2021_01_07_425723 897 8 Y. Y. NNP 10_1101-2021_01_07_425723 898 1 & & CC 10_1101-2021_01_07_425723 898 2 Smyth Smyth NNP 10_1101-2021_01_07_425723 898 3 , , , 10_1101-2021_01_07_425723 898 4 G. G. NNP 10_1101-2021_01_07_425723 898 5 K. K. NNP 10_1101-2021_01_07_425723 898 6 Differential Differential NNP 10_1101-2021_01_07_425723 898 7 expression expression NN 10_1101-2021_01_07_425723 898 8 analysis analysis NN 10_1101-2021_01_07_425723 898 9 of of IN 10_1101-2021_01_07_425723 898 10 multifactor multifactor NN 10_1101-2021_01_07_425723 898 11 RNA RNA NNP 10_1101-2021_01_07_425723 898 12 - - HYPH 10_1101-2021_01_07_425723 898 13 Seq Seq NNP 10_1101-2021_01_07_425723 898 14 899 899 CD 10_1101-2021_01_07_425723 898 15 experiments experiment NNS 10_1101-2021_01_07_425723 898 16 with with IN 10_1101-2021_01_07_425723 898 17 respect respect NN 10_1101-2021_01_07_425723 898 18 to to IN 10_1101-2021_01_07_425723 898 19 biological biological JJ 10_1101-2021_01_07_425723 898 20 variation variation NN 10_1101-2021_01_07_425723 898 21 . . . 10_1101-2021_01_07_425723 899 1 Nucleic Nucleic NNP 10_1101-2021_01_07_425723 899 2 Acids Acids NNPS 10_1101-2021_01_07_425723 899 3 Res Res NNP 10_1101-2021_01_07_425723 899 4 . . . 10_1101-2021_01_07_425723 900 1 40 40 CD 10_1101-2021_01_07_425723 900 2 , , , 10_1101-2021_01_07_425723 900 3 4288–4297 4288–4297 CD 10_1101-2021_01_07_425723 900 4 ( ( -LRB- 10_1101-2021_01_07_425723 900 5 2012 2012 CD 10_1101-2021_01_07_425723 900 6 ) ) -RRB- 10_1101-2021_01_07_425723 900 7 . . . 10_1101-2021_01_07_425723 901 1 900 900 CD 10_1101-2021_01_07_425723 901 2 80 80 CD 10_1101-2021_01_07_425723 901 3 . . . 10_1101-2021_01_07_425723 902 1 Robinson Robinson NNP 10_1101-2021_01_07_425723 902 2 , , , 10_1101-2021_01_07_425723 902 3 M. M. NNP 10_1101-2021_01_07_425723 902 4 D. D. NNP 10_1101-2021_01_07_425723 902 5 & & CC 10_1101-2021_01_07_425723 902 6 Oshlack Oshlack NNP 10_1101-2021_01_07_425723 902 7 , , , 10_1101-2021_01_07_425723 902 8 A. a. NN 10_1101-2021_01_07_425723 903 1 A a DT 10_1101-2021_01_07_425723 903 2 scaling scale VBG 10_1101-2021_01_07_425723 903 3 normalization normalization NN 10_1101-2021_01_07_425723 903 4 method method NN 10_1101-2021_01_07_425723 903 5 for for IN 10_1101-2021_01_07_425723 903 6 differential differential NN 10_1101-2021_01_07_425723 903 7 expression expression NN 10_1101-2021_01_07_425723 903 8 analysis analysis NN 10_1101-2021_01_07_425723 903 9 901 901 CD 10_1101-2021_01_07_425723 903 10 of of IN 10_1101-2021_01_07_425723 903 11 RNA RNA NNP 10_1101-2021_01_07_425723 903 12 - - HYPH 10_1101-2021_01_07_425723 903 13 seq seq NN 10_1101-2021_01_07_425723 903 14 data datum NNS 10_1101-2021_01_07_425723 903 15 . . . 10_1101-2021_01_07_425723 904 1 Genome Genome NNP 10_1101-2021_01_07_425723 904 2 Biol Biol NNP 10_1101-2021_01_07_425723 904 3 . . . 10_1101-2021_01_07_425723 905 1 11 11 CD 10_1101-2021_01_07_425723 905 2 , , , 10_1101-2021_01_07_425723 905 3 ( ( -LRB- 10_1101-2021_01_07_425723 905 4 2010 2010 CD 10_1101-2021_01_07_425723 905 5 ) ) -RRB- 10_1101-2021_01_07_425723 905 6 . . . 10_1101-2021_01_07_425723 906 1 902 902 CD 10_1101-2021_01_07_425723 906 2 81 81 CD 10_1101-2021_01_07_425723 906 3 . . . 10_1101-2021_01_07_425723 907 1 Gu Gu NNP 10_1101-2021_01_07_425723 907 2 , , , 10_1101-2021_01_07_425723 907 3 Z. Z. NNP 10_1101-2021_01_07_425723 907 4 , , , 10_1101-2021_01_07_425723 907 5 Eils Eils NNP 10_1101-2021_01_07_425723 907 6 , , , 10_1101-2021_01_07_425723 907 7 R. R. NNP 10_1101-2021_01_07_425723 907 8 & & CC 10_1101-2021_01_07_425723 907 9 Schlesner Schlesner NNP 10_1101-2021_01_07_425723 907 10 , , , 10_1101-2021_01_07_425723 907 11 M. M. NNP 10_1101-2021_01_07_425723 907 12 Complex Complex NNP 10_1101-2021_01_07_425723 907 13 heatmaps heatmap NNS 10_1101-2021_01_07_425723 907 14 reveal reveal VBP 10_1101-2021_01_07_425723 907 15 patterns pattern NNS 10_1101-2021_01_07_425723 907 16 and and CC 10_1101-2021_01_07_425723 907 17 correlations correlation NNS 10_1101-2021_01_07_425723 907 18 in in IN 10_1101-2021_01_07_425723 907 19 903 903 CD 10_1101-2021_01_07_425723 907 20 multidimensional multidimensional JJ 10_1101-2021_01_07_425723 907 21 genomic genomic JJ 10_1101-2021_01_07_425723 907 22 data datum NNS 10_1101-2021_01_07_425723 907 23 . . . 10_1101-2021_01_07_425723 908 1 Bioinformatics Bioinformatics NNP 10_1101-2021_01_07_425723 908 2 32 32 CD 10_1101-2021_01_07_425723 908 3 , , , 10_1101-2021_01_07_425723 908 4 2847–2849 2847–2849 CD 10_1101-2021_01_07_425723 908 5 ( ( -LRB- 10_1101-2021_01_07_425723 908 6 2016 2016 CD 10_1101-2021_01_07_425723 908 7 ) ) -RRB- 10_1101-2021_01_07_425723 908 8 . . . 10_1101-2021_01_07_425723 909 1 904 904 CD 10_1101-2021_01_07_425723 909 2 82 82 CD 10_1101-2021_01_07_425723 909 3 . . . 10_1101-2021_01_07_425723 910 1 R R NNP 10_1101-2021_01_07_425723 910 2 Core Core NNP 10_1101-2021_01_07_425723 910 3 Team Team NNP 10_1101-2021_01_07_425723 910 4 . . . 10_1101-2021_01_07_425723 911 1 R r NN 10_1101-2021_01_07_425723 911 2 : : : 10_1101-2021_01_07_425723 911 3 A A NNP 10_1101-2021_01_07_425723 911 4 Language Language NNP 10_1101-2021_01_07_425723 911 5 and and CC 10_1101-2021_01_07_425723 911 6 Environment Environment NNP 10_1101-2021_01_07_425723 911 7 for for IN 10_1101-2021_01_07_425723 911 8 Statistical Statistical NNP 10_1101-2021_01_07_425723 911 9 Computing Computing NNP 10_1101-2021_01_07_425723 911 10 . . . 10_1101-2021_01_07_425723 912 1 ( ( -LRB- 10_1101-2021_01_07_425723 912 2 2017 2017 CD 10_1101-2021_01_07_425723 912 3 ) ) -RRB- 10_1101-2021_01_07_425723 912 4 . . . 10_1101-2021_01_07_425723 913 1 905 905 CD 10_1101-2021_01_07_425723 913 2 83 83 CD 10_1101-2021_01_07_425723 913 3 . . . 10_1101-2021_01_07_425723 914 1 Juergens Juergens NNP 10_1101-2021_01_07_425723 914 2 , , , 10_1101-2021_01_07_425723 914 3 H. H. NNP 10_1101-2021_01_07_425723 914 4 et et NNP 10_1101-2021_01_07_425723 914 5 al al NNP 10_1101-2021_01_07_425723 914 6 . . . 10_1101-2021_01_07_425723 915 1 Evaluation evaluation NN 10_1101-2021_01_07_425723 915 2 of of IN 10_1101-2021_01_07_425723 915 3 a a DT 10_1101-2021_01_07_425723 915 4 novel novel JJ 10_1101-2021_01_07_425723 915 5 cloud cloud NN 10_1101-2021_01_07_425723 915 6 - - HYPH 10_1101-2021_01_07_425723 915 7 based base VBN 10_1101-2021_01_07_425723 915 8 software software NN 10_1101-2021_01_07_425723 915 9 platform platform NN 10_1101-2021_01_07_425723 915 10 for for IN 10_1101-2021_01_07_425723 915 11 structured structured JJ 10_1101-2021_01_07_425723 915 12 experiment experiment NN 10_1101-2021_01_07_425723 915 13 906 906 CD 10_1101-2021_01_07_425723 915 14 design design NN 10_1101-2021_01_07_425723 915 15 and and CC 10_1101-2021_01_07_425723 915 16 linked link VBD 10_1101-2021_01_07_425723 915 17 data datum NNS 10_1101-2021_01_07_425723 915 18 analytics analytic NNS 10_1101-2021_01_07_425723 915 19 . . . 10_1101-2021_01_07_425723 916 1 Sci Sci NNP 10_1101-2021_01_07_425723 916 2 . . . 10_1101-2021_01_07_425723 917 1 Data datum NNS 10_1101-2021_01_07_425723 917 2 5 5 CD 10_1101-2021_01_07_425723 917 3 , , , 10_1101-2021_01_07_425723 917 4 1–12 1–12 NNP 10_1101-2021_01_07_425723 917 5 ( ( -LRB- 10_1101-2021_01_07_425723 917 6 2018 2018 CD 10_1101-2021_01_07_425723 917 7 ) ) -RRB- 10_1101-2021_01_07_425723 917 8 . . . 10_1101-2021_01_07_425723 918 1 907 907 CD 10_1101-2021_01_07_425723 918 2 84 84 CD 10_1101-2021_01_07_425723 918 3 . . . 10_1101-2021_01_07_425723 919 1 Ortiz Ortiz NNP 10_1101-2021_01_07_425723 919 2 - - HYPH 10_1101-2021_01_07_425723 919 3 Merino Merino NNP 10_1101-2021_01_07_425723 919 4 , , , 10_1101-2021_01_07_425723 919 5 R. R. NNP 10_1101-2021_01_07_425723 919 6 A. A. NNP 10_1101-2021_01_07_425723 919 7 et et FW 10_1101-2021_01_07_425723 919 8 al al NNP 10_1101-2021_01_07_425723 919 9 . . . 10_1101-2021_01_07_425723 920 1 Ploidy Ploidy NNP 10_1101-2021_01_07_425723 920 2 Variation Variation NNP 10_1101-2021_01_07_425723 920 3 in in IN 10_1101-2021_01_07_425723 920 4 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 920 5 marxianus marxianus NN 10_1101-2021_01_07_425723 920 6 Separates Separates NNPS 10_1101-2021_01_07_425723 920 7 Dairy Dairy NNP 10_1101-2021_01_07_425723 920 8 and and CC 10_1101-2021_01_07_425723 920 9 Non-908 non-908 CD 10_1101-2021_01_07_425723 920 10 dairy dairy NN 10_1101-2021_01_07_425723 920 11 Isolates Isolates NNPS 10_1101-2021_01_07_425723 920 12 . . . 10_1101-2021_01_07_425723 921 1 Front front NN 10_1101-2021_01_07_425723 921 2 . . . 10_1101-2021_01_07_425723 922 1 Genet Genet NNP 10_1101-2021_01_07_425723 922 2 . . . 10_1101-2021_01_07_425723 923 1 9 9 CD 10_1101-2021_01_07_425723 923 2 , , , 10_1101-2021_01_07_425723 923 3 1–16 1–16 CD 10_1101-2021_01_07_425723 923 4 ( ( -LRB- 10_1101-2021_01_07_425723 923 5 2018 2018 CD 10_1101-2021_01_07_425723 923 6 ) ) -RRB- 10_1101-2021_01_07_425723 923 7 . . . 10_1101-2021_01_07_425723 924 1 909 909 CD 10_1101-2021_01_07_425723 924 2 85 85 CD 10_1101-2021_01_07_425723 924 3 . . . 10_1101-2021_01_07_425723 925 1 Li Li NNP 10_1101-2021_01_07_425723 925 2 , , , 10_1101-2021_01_07_425723 925 3 H. H. NNP 10_1101-2021_01_07_425723 925 4 & & CC 10_1101-2021_01_07_425723 925 5 Durbin Durbin NNP 10_1101-2021_01_07_425723 925 6 , , , 10_1101-2021_01_07_425723 925 7 R. R. NNP 10_1101-2021_01_07_425723 925 8 Fast Fast NNP 10_1101-2021_01_07_425723 925 9 and and CC 10_1101-2021_01_07_425723 925 10 accurate accurate JJ 10_1101-2021_01_07_425723 925 11 short short JJ 10_1101-2021_01_07_425723 925 12 read read NN 10_1101-2021_01_07_425723 925 13 alignment alignment NN 10_1101-2021_01_07_425723 925 14 with with IN 10_1101-2021_01_07_425723 925 15 Burrows Burrows NNP 10_1101-2021_01_07_425723 925 16 - - HYPH 10_1101-2021_01_07_425723 925 17 Wheeler Wheeler NNP 10_1101-2021_01_07_425723 925 18 transform transform NN 10_1101-2021_01_07_425723 925 19 . . . 10_1101-2021_01_07_425723 926 1 910 910 CD 10_1101-2021_01_07_425723 926 2 Bioinformatics Bioinformatics NNP 10_1101-2021_01_07_425723 926 3 25 25 CD 10_1101-2021_01_07_425723 926 4 , , , 10_1101-2021_01_07_425723 926 5 1754–1760 1754–1760 CD 10_1101-2021_01_07_425723 926 6 ( ( -LRB- 10_1101-2021_01_07_425723 926 7 2009 2009 CD 10_1101-2021_01_07_425723 926 8 ) ) -RRB- 10_1101-2021_01_07_425723 926 9 . . . 10_1101-2021_01_07_425723 927 1 911 911 CD 10_1101-2021_01_07_425723 927 2 86 86 CD 10_1101-2021_01_07_425723 927 3 . . . 10_1101-2021_01_07_425723 928 1 Auwera Auwera NNP 10_1101-2021_01_07_425723 928 2 , , , 10_1101-2021_01_07_425723 928 3 G. G. NNP 10_1101-2021_01_07_425723 928 4 A. A. NNP 10_1101-2021_01_07_425723 928 5 et et FW 10_1101-2021_01_07_425723 928 6 al al NNP 10_1101-2021_01_07_425723 928 7 . . . 10_1101-2021_01_07_425723 929 1 From from IN 10_1101-2021_01_07_425723 929 2 FastQ FastQ NNP 10_1101-2021_01_07_425723 929 3 Data Data NNP 10_1101-2021_01_07_425723 929 4 to to IN 10_1101-2021_01_07_425723 929 5 High high JJ 10_1101-2021_01_07_425723 929 6 - - HYPH 10_1101-2021_01_07_425723 929 7 Confidence confidence NN 10_1101-2021_01_07_425723 929 8 Variant Variant NNP 10_1101-2021_01_07_425723 929 9 Calls call NNS 10_1101-2021_01_07_425723 929 10 : : : 10_1101-2021_01_07_425723 929 11 The The NNP 10_1101-2021_01_07_425723 929 12 Genome Genome NNP 10_1101-2021_01_07_425723 929 13 Analysis Analysis NNP 10_1101-2021_01_07_425723 929 14 912 912 CD 10_1101-2021_01_07_425723 929 15 Toolkit Toolkit NNS 10_1101-2021_01_07_425723 929 16 Best Best NNP 10_1101-2021_01_07_425723 929 17 Practices Practices NNPS 10_1101-2021_01_07_425723 929 18 Pipeline Pipeline NNP 10_1101-2021_01_07_425723 929 19 . . . 10_1101-2021_01_07_425723 930 1 Curr curr UH 10_1101-2021_01_07_425723 930 2 . . . 10_1101-2021_01_07_425723 931 1 Protoc Protoc NNP 10_1101-2021_01_07_425723 931 2 . . . 10_1101-2021_01_07_425723 932 1 Bioinforma Bioinforma NNP 10_1101-2021_01_07_425723 932 2 . . . 10_1101-2021_01_07_425723 933 1 43 43 CD 10_1101-2021_01_07_425723 933 2 , , , 10_1101-2021_01_07_425723 933 3 11.10.1 11.10.1 CD 10_1101-2021_01_07_425723 933 4 - - SYM 10_1101-2021_01_07_425723 933 5 11.10.33 11.10.33 CD 10_1101-2021_01_07_425723 933 6 ( ( -LRB- 10_1101-2021_01_07_425723 933 7 2013 2013 CD 10_1101-2021_01_07_425723 933 8 ) ) -RRB- 10_1101-2021_01_07_425723 933 9 . . . 10_1101-2021_01_07_425723 934 1 913 913 CD 10_1101-2021_01_07_425723 934 2 87 87 CD 10_1101-2021_01_07_425723 934 3 . . . 10_1101-2021_01_07_425723 935 1 Cingolani Cingolani NNP 10_1101-2021_01_07_425723 935 2 , , , 10_1101-2021_01_07_425723 935 3 P. P. NNP 10_1101-2021_01_07_425723 935 4 et et NNP 10_1101-2021_01_07_425723 935 5 al al NNP 10_1101-2021_01_07_425723 935 6 . . . 10_1101-2021_01_07_425723 936 1 A a DT 10_1101-2021_01_07_425723 936 2 program program NN 10_1101-2021_01_07_425723 936 3 for for IN 10_1101-2021_01_07_425723 936 4 annotating annotate VBG 10_1101-2021_01_07_425723 936 5 and and CC 10_1101-2021_01_07_425723 936 6 predicting predict VBG 10_1101-2021_01_07_425723 936 7 the the DT 10_1101-2021_01_07_425723 936 8 effects effect NNS 10_1101-2021_01_07_425723 936 9 of of IN 10_1101-2021_01_07_425723 936 10 single single JJ 10_1101-2021_01_07_425723 936 11 nucleotide nucleotide JJ 10_1101-2021_01_07_425723 936 12 914 914 CD 10_1101-2021_01_07_425723 936 13 .CC .CC : 10_1101-2021_01_07_425723 936 14 - - HYPH 10_1101-2021_01_07_425723 936 15 BY by IN 10_1101-2021_01_07_425723 936 16 - - HYPH 10_1101-2021_01_07_425723 936 17 NC NC NNP 10_1101-2021_01_07_425723 936 18 - - HYPH 10_1101-2021_01_07_425723 936 19 ND ND NNP 10_1101-2021_01_07_425723 936 20 4.0 4.0 CD 10_1101-2021_01_07_425723 936 21 International International NNP 10_1101-2021_01_07_425723 936 22 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 936 23 under under IN 10_1101-2021_01_07_425723 936 24 a a DT 10_1101-2021_01_07_425723 936 25 ( ( -LRB- 10_1101-2021_01_07_425723 936 26 which which WDT 10_1101-2021_01_07_425723 936 27 was be VBD 10_1101-2021_01_07_425723 936 28 not not RB 10_1101-2021_01_07_425723 936 29 certified certify VBN 10_1101-2021_01_07_425723 936 30 by by IN 10_1101-2021_01_07_425723 936 31 peer peer NN 10_1101-2021_01_07_425723 936 32 review review NN 10_1101-2021_01_07_425723 936 33 ) ) -RRB- 10_1101-2021_01_07_425723 936 34 is be VBZ 10_1101-2021_01_07_425723 936 35 the the DT 10_1101-2021_01_07_425723 936 36 author author NN 10_1101-2021_01_07_425723 936 37 / / SYM 10_1101-2021_01_07_425723 936 38 funder funder NN 10_1101-2021_01_07_425723 936 39 , , , 10_1101-2021_01_07_425723 936 40 who who WP 10_1101-2021_01_07_425723 936 41 has have VBZ 10_1101-2021_01_07_425723 936 42 granted grant VBN 10_1101-2021_01_07_425723 936 43 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 936 44 a a DT 10_1101-2021_01_07_425723 936 45 license license NN 10_1101-2021_01_07_425723 936 46 to to TO 10_1101-2021_01_07_425723 936 47 display display VB 10_1101-2021_01_07_425723 936 48 the the DT 10_1101-2021_01_07_425723 936 49 preprint preprint NN 10_1101-2021_01_07_425723 936 50 in in IN 10_1101-2021_01_07_425723 936 51 perpetuity perpetuity NN 10_1101-2021_01_07_425723 936 52 . . . 10_1101-2021_01_07_425723 937 1 It -PRON- PRP 10_1101-2021_01_07_425723 937 2 is be VBZ 10_1101-2021_01_07_425723 937 3 made make VBN 10_1101-2021_01_07_425723 937 4 The the DT 10_1101-2021_01_07_425723 937 5 copyright copyright NN 10_1101-2021_01_07_425723 937 6 holder holder NN 10_1101-2021_01_07_425723 937 7 for for IN 10_1101-2021_01_07_425723 937 8 this this DT 10_1101-2021_01_07_425723 937 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 937 10 version version NN 10_1101-2021_01_07_425723 937 11 posted post VBD 10_1101-2021_01_07_425723 937 12 January January NNP 10_1101-2021_01_07_425723 937 13 8 8 CD 10_1101-2021_01_07_425723 937 14 , , , 10_1101-2021_01_07_425723 937 15 2021 2021 CD 10_1101-2021_01_07_425723 937 16 . . . 10_1101-2021_01_07_425723 937 17 ; ; : 10_1101-2021_01_07_425723 937 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 937 19 : : : 10_1101-2021_01_07_425723 937 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 937 21 preprint preprint NN 10_1101-2021_01_07_425723 937 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 937 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CC 10_1101-2021_01_07_425723 937 24 45 45 CD 10_1101-2021_01_07_425723 937 25 polymorphisms polymorphism NNS 10_1101-2021_01_07_425723 937 26 , , , 10_1101-2021_01_07_425723 937 27 SnpEff snpeff NN 10_1101-2021_01_07_425723 937 28 : : : 10_1101-2021_01_07_425723 937 29 SNPs snp NNS 10_1101-2021_01_07_425723 937 30 in in IN 10_1101-2021_01_07_425723 937 31 the the DT 10_1101-2021_01_07_425723 937 32 genome genome NN 10_1101-2021_01_07_425723 937 33 of of IN 10_1101-2021_01_07_425723 937 34 Drosophila Drosophila NNP 10_1101-2021_01_07_425723 937 35 melanogaster melanogaster NN 10_1101-2021_01_07_425723 937 36 strain strain NN 10_1101-2021_01_07_425723 937 37 w1118 w1118 NN 10_1101-2021_01_07_425723 937 38 ; ; : 10_1101-2021_01_07_425723 937 39 iso-2 iso-2 UH 10_1101-2021_01_07_425723 937 40 ; ; : 10_1101-2021_01_07_425723 937 41 iso-915 iso-915 NN 10_1101-2021_01_07_425723 937 42 3 3 CD 10_1101-2021_01_07_425723 937 43 . . . 10_1101-2021_01_07_425723 938 1 Fly Fly NNP 10_1101-2021_01_07_425723 938 2 ( ( -LRB- 10_1101-2021_01_07_425723 938 3 Austin Austin NNP 10_1101-2021_01_07_425723 938 4 ) ) -RRB- 10_1101-2021_01_07_425723 938 5 . . . 10_1101-2021_01_07_425723 939 1 6 6 CD 10_1101-2021_01_07_425723 939 2 , , , 10_1101-2021_01_07_425723 939 3 80–92 80–92 NNP 10_1101-2021_01_07_425723 939 4 ( ( -LRB- 10_1101-2021_01_07_425723 939 5 2012 2012 CD 10_1101-2021_01_07_425723 939 6 ) ) -RRB- 10_1101-2021_01_07_425723 939 7 . . . 10_1101-2021_01_07_425723 940 1 916 916 CD 10_1101-2021_01_07_425723 940 2 917 917 CD 10_1101-2021_01_07_425723 940 3 .CC .CC : 10_1101-2021_01_07_425723 940 4 - - HYPH 10_1101-2021_01_07_425723 940 5 BY by IN 10_1101-2021_01_07_425723 940 6 - - HYPH 10_1101-2021_01_07_425723 940 7 NC NC NNP 10_1101-2021_01_07_425723 940 8 - - HYPH 10_1101-2021_01_07_425723 940 9 ND ND NNP 10_1101-2021_01_07_425723 940 10 4.0 4.0 CD 10_1101-2021_01_07_425723 940 11 International International NNP 10_1101-2021_01_07_425723 940 12 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 940 13 under under IN 10_1101-2021_01_07_425723 940 14 a a DT 10_1101-2021_01_07_425723 940 15 ( ( -LRB- 10_1101-2021_01_07_425723 940 16 which which WDT 10_1101-2021_01_07_425723 940 17 was be VBD 10_1101-2021_01_07_425723 940 18 not not RB 10_1101-2021_01_07_425723 940 19 certified certify VBN 10_1101-2021_01_07_425723 940 20 by by IN 10_1101-2021_01_07_425723 940 21 peer peer NN 10_1101-2021_01_07_425723 940 22 review review NN 10_1101-2021_01_07_425723 940 23 ) ) -RRB- 10_1101-2021_01_07_425723 940 24 is be VBZ 10_1101-2021_01_07_425723 940 25 the the DT 10_1101-2021_01_07_425723 940 26 author author NN 10_1101-2021_01_07_425723 940 27 / / SYM 10_1101-2021_01_07_425723 940 28 funder funder NN 10_1101-2021_01_07_425723 940 29 , , , 10_1101-2021_01_07_425723 940 30 who who WP 10_1101-2021_01_07_425723 940 31 has have VBZ 10_1101-2021_01_07_425723 940 32 granted grant VBN 10_1101-2021_01_07_425723 940 33 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 940 34 a a DT 10_1101-2021_01_07_425723 940 35 license license NN 10_1101-2021_01_07_425723 940 36 to to TO 10_1101-2021_01_07_425723 940 37 display display VB 10_1101-2021_01_07_425723 940 38 the the DT 10_1101-2021_01_07_425723 940 39 preprint preprint NN 10_1101-2021_01_07_425723 940 40 in in IN 10_1101-2021_01_07_425723 940 41 perpetuity perpetuity NN 10_1101-2021_01_07_425723 940 42 . . . 10_1101-2021_01_07_425723 941 1 It -PRON- PRP 10_1101-2021_01_07_425723 941 2 is be VBZ 10_1101-2021_01_07_425723 941 3 made make VBN 10_1101-2021_01_07_425723 941 4 The the DT 10_1101-2021_01_07_425723 941 5 copyright copyright NN 10_1101-2021_01_07_425723 941 6 holder holder NN 10_1101-2021_01_07_425723 941 7 for for IN 10_1101-2021_01_07_425723 941 8 this this DT 10_1101-2021_01_07_425723 941 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 941 10 version version NN 10_1101-2021_01_07_425723 941 11 posted post VBD 10_1101-2021_01_07_425723 941 12 January January NNP 10_1101-2021_01_07_425723 941 13 8 8 CD 10_1101-2021_01_07_425723 941 14 , , , 10_1101-2021_01_07_425723 941 15 2021 2021 CD 10_1101-2021_01_07_425723 941 16 . . . 10_1101-2021_01_07_425723 941 17 ; ; : 10_1101-2021_01_07_425723 941 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 941 19 : : : 10_1101-2021_01_07_425723 941 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 941 21 preprint preprint NN 10_1101-2021_01_07_425723 941 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 941 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 941 24 46 46 CD 10_1101-2021_01_07_425723 941 25 Description description NN 10_1101-2021_01_07_425723 941 26 of of IN 10_1101-2021_01_07_425723 941 27 Additional Additional NNP 10_1101-2021_01_07_425723 941 28 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 941 29 Files Files NNP 10_1101-2021_01_07_425723 941 30 918 918 CD 10_1101-2021_01_07_425723 941 31 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 941 32 Data Data NNP 10_1101-2021_01_07_425723 941 33 Set Set NNP 10_1101-2021_01_07_425723 941 34 1 1 CD 10_1101-2021_01_07_425723 941 35 | | NNP 10_1101-2021_01_07_425723 941 36 Overview Overview NNP 10_1101-2021_01_07_425723 941 37 of of IN 10_1101-2021_01_07_425723 941 38 flow flow NN 10_1101-2021_01_07_425723 941 39 cytometry cytometry NN 10_1101-2021_01_07_425723 941 40 samples sample NNS 10_1101-2021_01_07_425723 941 41 with with IN 10_1101-2021_01_07_425723 941 42 meta meta JJ 10_1101-2021_01_07_425723 941 43 - - HYPH 10_1101-2021_01_07_425723 941 44 data datum NNS 10_1101-2021_01_07_425723 941 45 . . . 10_1101-2021_01_07_425723 942 1 Meta meta JJ 10_1101-2021_01_07_425723 942 2 - - HYPH 10_1101-2021_01_07_425723 942 3 data datum NNS 10_1101-2021_01_07_425723 942 4 Table table NN 10_1101-2021_01_07_425723 942 5 of of IN 10_1101-2021_01_07_425723 942 6 919 919 CD 10_1101-2021_01_07_425723 942 7 file file NN 10_1101-2021_01_07_425723 942 8 names name NNS 10_1101-2021_01_07_425723 942 9 , , , 10_1101-2021_01_07_425723 942 10 frequency frequency NN 10_1101-2021_01_07_425723 942 11 of of IN 10_1101-2021_01_07_425723 942 12 cells cell NNS 10_1101-2021_01_07_425723 942 13 compared compare VBN 10_1101-2021_01_07_425723 942 14 to to IN 10_1101-2021_01_07_425723 942 15 parent parent NN 10_1101-2021_01_07_425723 942 16 , , , 10_1101-2021_01_07_425723 942 17 number number NN 10_1101-2021_01_07_425723 942 18 of of IN 10_1101-2021_01_07_425723 942 19 cells cell NNS 10_1101-2021_01_07_425723 942 20 in in IN 10_1101-2021_01_07_425723 942 21 each each DT 10_1101-2021_01_07_425723 942 22 group group NN 10_1101-2021_01_07_425723 942 23 , , , 10_1101-2021_01_07_425723 942 24 strain strain VB 10_1101-2021_01_07_425723 942 25 name name NN 10_1101-2021_01_07_425723 942 26 , , , 10_1101-2021_01_07_425723 942 27 time time NN 10_1101-2021_01_07_425723 942 28 920 920 CD 10_1101-2021_01_07_425723 942 29 point point NN 10_1101-2021_01_07_425723 942 30 of of IN 10_1101-2021_01_07_425723 942 31 fluorescence fluorescence NN 10_1101-2021_01_07_425723 942 32 measurement measurement NN 10_1101-2021_01_07_425723 942 33 after after IN 10_1101-2021_01_07_425723 942 34 4 4 CD 10_1101-2021_01_07_425723 942 35 hours hour NNS 10_1101-2021_01_07_425723 942 36 ( ( -LRB- 10_1101-2021_01_07_425723 942 37 1 1 CD 10_1101-2021_01_07_425723 942 38 ) ) -RRB- 10_1101-2021_01_07_425723 942 39 or or CC 10_1101-2021_01_07_425723 942 40 23 23 CD 10_1101-2021_01_07_425723 942 41 hours hour NNS 10_1101-2021_01_07_425723 942 42 ( ( -LRB- 10_1101-2021_01_07_425723 942 43 2 2 CD 10_1101-2021_01_07_425723 942 44 ) ) -RRB- 10_1101-2021_01_07_425723 942 45 , , , 10_1101-2021_01_07_425723 942 46 staining stain VBG 10_1101-2021_01_07_425723 942 47 of of IN 10_1101-2021_01_07_425723 942 48 cells cell NNS 10_1101-2021_01_07_425723 942 49 with with IN 10_1101-2021_01_07_425723 942 50 propidium-921 propidium-921 NNP 10_1101-2021_01_07_425723 942 51 iodide iodide NNP 10_1101-2021_01_07_425723 942 52 ( ( -LRB- 10_1101-2021_01_07_425723 942 53 PI PI NNP 10_1101-2021_01_07_425723 942 54 ) ) -RRB- 10_1101-2021_01_07_425723 942 55 with with IN 10_1101-2021_01_07_425723 942 56 value value NN 10_1101-2021_01_07_425723 942 57 ( ( -LRB- 10_1101-2021_01_07_425723 942 58 PI PI NNP 10_1101-2021_01_07_425723 942 59 ) ) -RRB- 10_1101-2021_01_07_425723 942 60 or or CC 10_1101-2021_01_07_425723 942 61 without without IN 10_1101-2021_01_07_425723 942 62 PI PI NNP 10_1101-2021_01_07_425723 942 63 staining staining NN 10_1101-2021_01_07_425723 942 64 ( ( -LRB- 10_1101-2021_01_07_425723 942 65 - - HYPH 10_1101-2021_01_07_425723 942 66 ) ) -RRB- 10_1101-2021_01_07_425723 942 67 , , , 10_1101-2021_01_07_425723 942 68 staining stain VBG 10_1101-2021_01_07_425723 942 69 of of IN 10_1101-2021_01_07_425723 942 70 cells cell NNS 10_1101-2021_01_07_425723 942 71 with with IN 10_1101-2021_01_07_425723 942 72 Tween Tween NNP 10_1101-2021_01_07_425723 942 73 80 80 CD 10_1101-2021_01_07_425723 942 74 NBD NBD NNP 10_1101-2021_01_07_425723 942 75 - - HYPH 10_1101-2021_01_07_425723 942 76 cholesterol cholesterol NN 10_1101-2021_01_07_425723 942 77 ( ( -LRB- 10_1101-2021_01_07_425723 942 78 TN TN NNP 10_1101-2021_01_07_425723 942 79 ) ) -RRB- 10_1101-2021_01_07_425723 942 80 922 922 CD 10_1101-2021_01_07_425723 942 81 or or CC 10_1101-2021_01_07_425723 942 82 with with IN 10_1101-2021_01_07_425723 942 83 Tween Tween NNP 10_1101-2021_01_07_425723 942 84 80 80 CD 10_1101-2021_01_07_425723 942 85 only only RB 10_1101-2021_01_07_425723 942 86 ( ( -LRB- 10_1101-2021_01_07_425723 942 87 T t NN 10_1101-2021_01_07_425723 942 88 ) ) -RRB- 10_1101-2021_01_07_425723 942 89 , , , 10_1101-2021_01_07_425723 942 90 with with IN 10_1101-2021_01_07_425723 942 91 species specie NNS 10_1101-2021_01_07_425723 942 92 names name NNS 10_1101-2021_01_07_425723 942 93 abbreviated abbreviate VBD 10_1101-2021_01_07_425723 942 94 K. K. NNP 10_1101-2021_01_07_425723 942 95 marxianus marxianus NN 10_1101-2021_01_07_425723 942 96 ( ( -LRB- 10_1101-2021_01_07_425723 942 97 Km Km NNP 10_1101-2021_01_07_425723 942 98 ) ) -RRB- 10_1101-2021_01_07_425723 942 99 or or CC 10_1101-2021_01_07_425723 942 100 S. S. NNP 10_1101-2021_01_07_425723 942 101 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 942 102 ( ( -LRB- 10_1101-2021_01_07_425723 942 103 Sc Sc NNP 10_1101-2021_01_07_425723 942 104 ) ) -RRB- 10_1101-2021_01_07_425723 942 105 . . . 10_1101-2021_01_07_425723 943 1 923 923 CD 10_1101-2021_01_07_425723 943 2 [ [ -LRB- 10_1101-2021_01_07_425723 943 3 Example example NN 10_1101-2021_01_07_425723 943 4 picture picture NN 10_1101-2021_01_07_425723 943 5 of of IN 10_1101-2021_01_07_425723 943 6 file file NN 10_1101-2021_01_07_425723 943 7 FlowCyto_Table.xlsx FlowCyto_Table.xlsx . 10_1101-2021_01_07_425723 943 8 ] ] -RRB- 10_1101-2021_01_07_425723 943 9 924 924 CD 10_1101-2021_01_07_425723 943 10 925 925 CD 10_1101-2021_01_07_425723 943 11 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 943 12 Data Data NNP 10_1101-2021_01_07_425723 943 13 set set NN 10_1101-2021_01_07_425723 943 14 2 2 CD 10_1101-2021_01_07_425723 943 15 | | CD 10_1101-2021_01_07_425723 943 16 Flow Flow NNP 10_1101-2021_01_07_425723 943 17 cytometry cytometry NN 10_1101-2021_01_07_425723 943 18 non non JJ 10_1101-2021_01_07_425723 943 19 - - JJ 10_1101-2021_01_07_425723 943 20 gated gated JJ 10_1101-2021_01_07_425723 943 21 data datum NNS 10_1101-2021_01_07_425723 943 22 of of IN 10_1101-2021_01_07_425723 943 23 FL3-A FL3-A NNP 10_1101-2021_01_07_425723 943 24 versus versus IN 10_1101-2021_01_07_425723 943 25 FL1-A FL1-A NNP 10_1101-2021_01_07_425723 943 26 of of IN 10_1101-2021_01_07_425723 943 27 all all DT 10_1101-2021_01_07_425723 943 28 samples sample NNS 10_1101-2021_01_07_425723 943 29 . . . 10_1101-2021_01_07_425723 944 1 926 926 CD 10_1101-2021_01_07_425723 944 2 Flow Flow NNP 10_1101-2021_01_07_425723 944 3 cytometry cytometry NN 10_1101-2021_01_07_425723 944 4 data datum NNS 10_1101-2021_01_07_425723 944 5 of of IN 10_1101-2021_01_07_425723 944 6 showing show VBG 10_1101-2021_01_07_425723 944 7 fluorescent fluorescent NN 10_1101-2021_01_07_425723 944 8 NBDC NBDC NNP 10_1101-2021_01_07_425723 944 9 uptake uptake JJ 10_1101-2021_01_07_425723 944 10 by by IN 10_1101-2021_01_07_425723 944 11 K. K. NNP 10_1101-2021_01_07_425723 944 12 marxianus marxianus NN 10_1101-2021_01_07_425723 944 13 , , , 10_1101-2021_01_07_425723 944 14 S. S. NNP 10_1101-2021_01_07_425723 944 15 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 944 16 strains strain VBZ 10_1101-2021_01_07_425723 944 17 with with IN 10_1101-2021_01_07_425723 944 18 for for IN 10_1101-2021_01_07_425723 944 19 927 927 CD 10_1101-2021_01_07_425723 944 20 each each DT 10_1101-2021_01_07_425723 944 21 sample sample NN 10_1101-2021_01_07_425723 944 22 the the DT 10_1101-2021_01_07_425723 944 23 intensity intensity NN 10_1101-2021_01_07_425723 944 24 of of IN 10_1101-2021_01_07_425723 944 25 counts count NNS 10_1101-2021_01_07_425723 944 26 ( ( -LRB- 10_1101-2021_01_07_425723 944 27 pseudo pseudo NNP 10_1101-2021_01_07_425723 944 28 - - JJ 10_1101-2021_01_07_425723 944 29 colored colored JJ 10_1101-2021_01_07_425723 944 30 ) ) -RRB- 10_1101-2021_01_07_425723 944 31 for for IN 10_1101-2021_01_07_425723 944 32 533/30 533/30 CD 10_1101-2021_01_07_425723 944 33 nm nm IN 10_1101-2021_01_07_425723 944 34 ( ( -LRB- 10_1101-2021_01_07_425723 944 35 FL1 FL1 NNP 10_1101-2021_01_07_425723 944 36 ) ) -RRB- 10_1101-2021_01_07_425723 944 37 for for IN 10_1101-2021_01_07_425723 944 38 NBDC NBDC NNP 10_1101-2021_01_07_425723 944 39 and and CC 10_1101-2021_01_07_425723 944 40 > > XX 10_1101-2021_01_07_425723 944 41 670 670 CD 10_1101-2021_01_07_425723 944 42 nm nm CD 10_1101-2021_01_07_425723 944 43 ( ( -LRB- 10_1101-2021_01_07_425723 944 44 FL3 FL3 NNP 10_1101-2021_01_07_425723 944 45 ) ) -RRB- 10_1101-2021_01_07_425723 944 46 928 928 CD 10_1101-2021_01_07_425723 944 47 for for IN 10_1101-2021_01_07_425723 944 48 PI PI NNP 10_1101-2021_01_07_425723 944 49 . . . 10_1101-2021_01_07_425723 945 1 929 929 CD 10_1101-2021_01_07_425723 945 2 [ [ -LRB- 10_1101-2021_01_07_425723 945 3 Example example NN 10_1101-2021_01_07_425723 945 4 of of IN 10_1101-2021_01_07_425723 945 5 first first JJ 10_1101-2021_01_07_425723 945 6 row row NN 10_1101-2021_01_07_425723 945 7 of of IN 10_1101-2021_01_07_425723 945 8 FlowCyto_FL1_FL3.pdf flowcyto_fl1_fl3.pdf NN 10_1101-2021_01_07_425723 945 9 ] ] -RRB- 10_1101-2021_01_07_425723 945 10 930 930 CD 10_1101-2021_01_07_425723 945 11 Filename Filename NNP 10_1101-2021_01_07_425723 945 12 Strain Strain NNP 10_1101-2021_01_07_425723 945 13 Time Time NNP 10_1101-2021_01_07_425723 945 14 point point NN 10_1101-2021_01_07_425723 945 15 PI pi NN 10_1101-2021_01_07_425723 945 16 # # $ 10_1101-2021_01_07_425723 945 17 Day Day NNP 10_1101-2021_01_07_425723 945 18 Staining Staining NNP 10_1101-2021_01_07_425723 945 19 Cells Cells NNPS 10_1101-2021_01_07_425723 945 20 / / SYM 10_1101-2021_01_07_425723 945 21 PI PI NNP 10_1101-2021_01_07_425723 945 22 - - HYPH 10_1101-2021_01_07_425723 945 23 ne ne NNP 10_1101-2021_01_07_425723 945 24 Cells Cells NNPS 10_1101-2021_01_07_425723 945 25 / / SYM 10_1101-2021_01_07_425723 945 26 PI PI NNP 10_1101-2021_01_07_425723 945 27 - - HYPH 10_1101-2021_01_07_425723 945 28 po po NNP 10_1101-2021_01_07_425723 945 29 Cells Cells NNPS 10_1101-2021_01_07_425723 945 30 / / SYM 10_1101-2021_01_07_425723 945 31 PI PI NNP 10_1101-2021_01_07_425723 945 32 - - HYPH 10_1101-2021_01_07_425723 945 33 ne ne NNP 10_1101-2021_01_07_425723 945 34 A09 A09 NNP 10_1101-2021_01_07_425723 945 35 CBS6556_T_A_PI_1.fcs CBS6556_T_A_PI_1.fcs NNP 10_1101-2021_01_07_425723 945 36 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 945 37 1 1 CD 10_1101-2021_01_07_425723 945 38 PI PI NNP 10_1101-2021_01_07_425723 945 39 A a NN 10_1101-2021_01_07_425723 945 40 1 1 CD 10_1101-2021_01_07_425723 945 41 T t NN 10_1101-2021_01_07_425723 945 42 576 576 CD 10_1101-2021_01_07_425723 945 43 411000 411000 CD 10_1101-2021_01_07_425723 945 44 75590 75590 CD 10_1101-2021_01_07_425723 945 45 B09 B09 NNP 10_1101-2021_01_07_425723 945 46 CBS6556_T_B_PI_1.fcs CBS6556_T_B_PI_1.fcs NNP 10_1101-2021_01_07_425723 945 47 CBS6556 CBS6556 NNP 10_1101-2021_01_07_425723 945 48 1 1 CD 10_1101-2021_01_07_425723 945 49 PI PI NNP 10_1101-2021_01_07_425723 945 50 B B NNP 10_1101-2021_01_07_425723 945 51 1 1 CD 10_1101-2021_01_07_425723 945 52 T t NN 10_1101-2021_01_07_425723 945 53 625 625 CD 10_1101-2021_01_07_425723 945 54 398024 398024 CD 10_1101-2021_01_07_425723 945 55 88212 88212 CD 10_1101-2021_01_07_425723 945 56 A01 A01 NNP 10_1101-2021_01_07_425723 945 57 IMX585_T_A___1.fcs IMX585_T_A___1.fcs NNP 10_1101-2021_01_07_425723 945 58 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 945 59 1 1 CD 10_1101-2021_01_07_425723 945 60 - - HYPH 10_1101-2021_01_07_425723 945 61 A a NN 10_1101-2021_01_07_425723 945 62 2 2 CD 10_1101-2021_01_07_425723 945 63 T t NN 10_1101-2021_01_07_425723 945 64 1391 1391 CD 10_1101-2021_01_07_425723 945 65 3 3 CD 10_1101-2021_01_07_425723 945 66 472000 472000 CD 10_1101-2021_01_07_425723 945 67 .CC .CC : 10_1101-2021_01_07_425723 945 68 - - HYPH 10_1101-2021_01_07_425723 945 69 BY by IN 10_1101-2021_01_07_425723 945 70 - - HYPH 10_1101-2021_01_07_425723 945 71 NC NC NNP 10_1101-2021_01_07_425723 945 72 - - HYPH 10_1101-2021_01_07_425723 945 73 ND ND NNP 10_1101-2021_01_07_425723 945 74 4.0 4.0 CD 10_1101-2021_01_07_425723 945 75 International International NNP 10_1101-2021_01_07_425723 945 76 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 945 77 under under IN 10_1101-2021_01_07_425723 945 78 a a DT 10_1101-2021_01_07_425723 945 79 ( ( -LRB- 10_1101-2021_01_07_425723 945 80 which which WDT 10_1101-2021_01_07_425723 945 81 was be VBD 10_1101-2021_01_07_425723 945 82 not not RB 10_1101-2021_01_07_425723 945 83 certified certify VBN 10_1101-2021_01_07_425723 945 84 by by IN 10_1101-2021_01_07_425723 945 85 peer peer NN 10_1101-2021_01_07_425723 945 86 review review NN 10_1101-2021_01_07_425723 945 87 ) ) -RRB- 10_1101-2021_01_07_425723 945 88 is be VBZ 10_1101-2021_01_07_425723 945 89 the the DT 10_1101-2021_01_07_425723 945 90 author author NN 10_1101-2021_01_07_425723 945 91 / / SYM 10_1101-2021_01_07_425723 945 92 funder funder NN 10_1101-2021_01_07_425723 945 93 , , , 10_1101-2021_01_07_425723 945 94 who who WP 10_1101-2021_01_07_425723 945 95 has have VBZ 10_1101-2021_01_07_425723 945 96 granted grant VBN 10_1101-2021_01_07_425723 945 97 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 945 98 a a DT 10_1101-2021_01_07_425723 945 99 license license NN 10_1101-2021_01_07_425723 945 100 to to TO 10_1101-2021_01_07_425723 945 101 display display VB 10_1101-2021_01_07_425723 945 102 the the DT 10_1101-2021_01_07_425723 945 103 preprint preprint NN 10_1101-2021_01_07_425723 945 104 in in IN 10_1101-2021_01_07_425723 945 105 perpetuity perpetuity NN 10_1101-2021_01_07_425723 945 106 . . . 10_1101-2021_01_07_425723 946 1 It -PRON- PRP 10_1101-2021_01_07_425723 946 2 is be VBZ 10_1101-2021_01_07_425723 946 3 made make VBN 10_1101-2021_01_07_425723 946 4 The the DT 10_1101-2021_01_07_425723 946 5 copyright copyright NN 10_1101-2021_01_07_425723 946 6 holder holder NN 10_1101-2021_01_07_425723 946 7 for for IN 10_1101-2021_01_07_425723 946 8 this this DT 10_1101-2021_01_07_425723 946 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 946 10 version version NN 10_1101-2021_01_07_425723 946 11 posted post VBD 10_1101-2021_01_07_425723 946 12 January January NNP 10_1101-2021_01_07_425723 946 13 8 8 CD 10_1101-2021_01_07_425723 946 14 , , , 10_1101-2021_01_07_425723 946 15 2021 2021 CD 10_1101-2021_01_07_425723 946 16 . . . 10_1101-2021_01_07_425723 946 17 ; ; : 10_1101-2021_01_07_425723 946 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 946 19 : : : 10_1101-2021_01_07_425723 946 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 946 21 preprint preprint NN 10_1101-2021_01_07_425723 946 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 946 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 946 24 47 47 CD 10_1101-2021_01_07_425723 946 25 931 931 CD 10_1101-2021_01_07_425723 946 26 .CC .CC : 10_1101-2021_01_07_425723 946 27 - - HYPH 10_1101-2021_01_07_425723 946 28 BY by IN 10_1101-2021_01_07_425723 946 29 - - HYPH 10_1101-2021_01_07_425723 946 30 NC NC NNP 10_1101-2021_01_07_425723 946 31 - - HYPH 10_1101-2021_01_07_425723 946 32 ND ND NNP 10_1101-2021_01_07_425723 946 33 4.0 4.0 CD 10_1101-2021_01_07_425723 946 34 International International NNP 10_1101-2021_01_07_425723 946 35 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 946 36 under under IN 10_1101-2021_01_07_425723 946 37 a a DT 10_1101-2021_01_07_425723 946 38 ( ( -LRB- 10_1101-2021_01_07_425723 946 39 which which WDT 10_1101-2021_01_07_425723 946 40 was be VBD 10_1101-2021_01_07_425723 946 41 not not RB 10_1101-2021_01_07_425723 946 42 certified certify VBN 10_1101-2021_01_07_425723 946 43 by by IN 10_1101-2021_01_07_425723 946 44 peer peer NN 10_1101-2021_01_07_425723 946 45 review review NN 10_1101-2021_01_07_425723 946 46 ) ) -RRB- 10_1101-2021_01_07_425723 946 47 is be VBZ 10_1101-2021_01_07_425723 946 48 the the DT 10_1101-2021_01_07_425723 946 49 author author NN 10_1101-2021_01_07_425723 946 50 / / SYM 10_1101-2021_01_07_425723 946 51 funder funder NN 10_1101-2021_01_07_425723 946 52 , , , 10_1101-2021_01_07_425723 946 53 who who WP 10_1101-2021_01_07_425723 946 54 has have VBZ 10_1101-2021_01_07_425723 946 55 granted grant VBN 10_1101-2021_01_07_425723 946 56 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 946 57 a a DT 10_1101-2021_01_07_425723 946 58 license license NN 10_1101-2021_01_07_425723 946 59 to to TO 10_1101-2021_01_07_425723 946 60 display display VB 10_1101-2021_01_07_425723 946 61 the the DT 10_1101-2021_01_07_425723 946 62 preprint preprint NN 10_1101-2021_01_07_425723 946 63 in in IN 10_1101-2021_01_07_425723 946 64 perpetuity perpetuity NN 10_1101-2021_01_07_425723 946 65 . . . 10_1101-2021_01_07_425723 947 1 It -PRON- PRP 10_1101-2021_01_07_425723 947 2 is be VBZ 10_1101-2021_01_07_425723 947 3 made make VBN 10_1101-2021_01_07_425723 947 4 The the DT 10_1101-2021_01_07_425723 947 5 copyright copyright NN 10_1101-2021_01_07_425723 947 6 holder holder NN 10_1101-2021_01_07_425723 947 7 for for IN 10_1101-2021_01_07_425723 947 8 this this DT 10_1101-2021_01_07_425723 947 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 947 10 version version NN 10_1101-2021_01_07_425723 947 11 posted post VBD 10_1101-2021_01_07_425723 947 12 January January NNP 10_1101-2021_01_07_425723 947 13 8 8 CD 10_1101-2021_01_07_425723 947 14 , , , 10_1101-2021_01_07_425723 947 15 2021 2021 CD 10_1101-2021_01_07_425723 947 16 . . . 10_1101-2021_01_07_425723 947 17 ; ; : 10_1101-2021_01_07_425723 947 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 947 19 : : : 10_1101-2021_01_07_425723 947 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 947 21 preprint preprint NN 10_1101-2021_01_07_425723 947 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 947 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 947 24 50 50 CD 10_1101-2021_01_07_425723 947 25 Supplemental supplemental JJ 10_1101-2021_01_07_425723 947 26 material material NN 10_1101-2021_01_07_425723 947 27 for for IN 10_1101-2021_01_07_425723 947 28 : : : 10_1101-2021_01_07_425723 947 29 934 934 CD 10_1101-2021_01_07_425723 947 30 Engineering engineer VBG 10_1101-2021_01_07_425723 947 31 the the DT 10_1101-2021_01_07_425723 947 32 thermotolerant thermotolerant JJ 10_1101-2021_01_07_425723 947 33 industrial industrial JJ 10_1101-2021_01_07_425723 947 34 yeast yeast NN 10_1101-2021_01_07_425723 947 35 Kluyveromyces Kluyveromyces NNP 10_1101-2021_01_07_425723 947 36 marxianus marxianus NN 10_1101-2021_01_07_425723 947 37 for for IN 10_1101-2021_01_07_425723 947 38 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 947 39 growth growth NN 10_1101-2021_01_07_425723 947 40 935 935 CD 10_1101-2021_01_07_425723 947 41 Wijbrand Wijbrand NNP 10_1101-2021_01_07_425723 947 42 J. J. NNP 10_1101-2021_01_07_425723 947 43 C. C. NNP 10_1101-2021_01_07_425723 947 44 Dekker Dekker NNP 10_1101-2021_01_07_425723 947 45 , , , 10_1101-2021_01_07_425723 947 46 Raúl Raúl NNP 10_1101-2021_01_07_425723 947 47 A. A. NNP 10_1101-2021_01_07_425723 947 48 Ortiz Ortiz NNP 10_1101-2021_01_07_425723 947 49 - - HYPH 10_1101-2021_01_07_425723 947 50 Merino Merino NNP 10_1101-2021_01_07_425723 947 51 , , , 10_1101-2021_01_07_425723 947 52 Astrid Astrid NNP 10_1101-2021_01_07_425723 947 53 Kaljouw Kaljouw NNP 10_1101-2021_01_07_425723 947 54 , , , 10_1101-2021_01_07_425723 947 55 Julius Julius NNP 10_1101-2021_01_07_425723 947 56 Battjes Battjes NNP 10_1101-2021_01_07_425723 947 57 , , , 10_1101-2021_01_07_425723 947 58 Frank Frank NNP 10_1101-2021_01_07_425723 947 59 Wiering Wiering NNP 10_1101-2021_01_07_425723 947 60 , , , 10_1101-2021_01_07_425723 947 61 Christiaan Christiaan NNP 10_1101-2021_01_07_425723 947 62 936 936 CD 10_1101-2021_01_07_425723 947 63 Mooiman Mooiman NNP 10_1101-2021_01_07_425723 947 64 , , , 10_1101-2021_01_07_425723 947 65 Pilar Pilar NNP 10_1101-2021_01_07_425723 947 66 de de NNP 10_1101-2021_01_07_425723 947 67 la la NNP 10_1101-2021_01_07_425723 947 68 Torre Torre NNP 10_1101-2021_01_07_425723 947 69 , , , 10_1101-2021_01_07_425723 947 70 and and CC 10_1101-2021_01_07_425723 947 71 Jack Jack NNP 10_1101-2021_01_07_425723 947 72 T. T. NNP 10_1101-2021_01_07_425723 947 73 Pronk Pronk NNP 10_1101-2021_01_07_425723 947 74 * * NFP 10_1101-2021_01_07_425723 947 75 937 937 CD 10_1101-2021_01_07_425723 947 76 Department Department NNP 10_1101-2021_01_07_425723 947 77 of of IN 10_1101-2021_01_07_425723 947 78 Biotechnology Biotechnology NNP 10_1101-2021_01_07_425723 947 79 , , , 10_1101-2021_01_07_425723 947 80 Delft Delft NNP 10_1101-2021_01_07_425723 947 81 University University NNP 10_1101-2021_01_07_425723 947 82 of of IN 10_1101-2021_01_07_425723 947 83 Technology Technology NNP 10_1101-2021_01_07_425723 947 84 , , , 10_1101-2021_01_07_425723 947 85 van van NNP 10_1101-2021_01_07_425723 947 86 der der NNP 10_1101-2021_01_07_425723 947 87 Maasweg Maasweg NNP 10_1101-2021_01_07_425723 947 88 9 9 CD 10_1101-2021_01_07_425723 947 89 , , , 10_1101-2021_01_07_425723 947 90 2629 2629 CD 10_1101-2021_01_07_425723 947 91 HZ HZ NNP 10_1101-2021_01_07_425723 947 92 Delft Delft NNP 10_1101-2021_01_07_425723 947 93 , , , 10_1101-2021_01_07_425723 947 94 The the DT 10_1101-2021_01_07_425723 947 95 938 938 CD 10_1101-2021_01_07_425723 947 96 Netherlands Netherlands NNP 10_1101-2021_01_07_425723 947 97 939 939 CD 10_1101-2021_01_07_425723 947 98 * * NFP 10_1101-2021_01_07_425723 947 99 Corresponding Corresponding NNP 10_1101-2021_01_07_425723 947 100 author author NN 10_1101-2021_01_07_425723 947 101 : : : 10_1101-2021_01_07_425723 947 102 Department Department NNP 10_1101-2021_01_07_425723 947 103 of of IN 10_1101-2021_01_07_425723 947 104 Biotechnology Biotechnology NNP 10_1101-2021_01_07_425723 947 105 , , , 10_1101-2021_01_07_425723 947 106 Delft Delft NNP 10_1101-2021_01_07_425723 947 107 University University NNP 10_1101-2021_01_07_425723 947 108 of of IN 10_1101-2021_01_07_425723 947 109 Technology Technology NNP 10_1101-2021_01_07_425723 947 110 , , , 10_1101-2021_01_07_425723 947 111 Van Van NNP 10_1101-2021_01_07_425723 947 112 der der XX 10_1101-2021_01_07_425723 947 113 Maasweg Maasweg NNP 10_1101-2021_01_07_425723 947 114 940 940 CD 10_1101-2021_01_07_425723 947 115 9 9 CD 10_1101-2021_01_07_425723 947 116 , , , 10_1101-2021_01_07_425723 947 117 2629 2629 CD 10_1101-2021_01_07_425723 947 118 HZ HZ NNP 10_1101-2021_01_07_425723 947 119 Delft Delft NNP 10_1101-2021_01_07_425723 947 120 , , , 10_1101-2021_01_07_425723 947 121 The the DT 10_1101-2021_01_07_425723 947 122 Netherlands Netherlands NNP 10_1101-2021_01_07_425723 947 123 , , , 10_1101-2021_01_07_425723 947 124 E e NN 10_1101-2021_01_07_425723 947 125 - - NN 10_1101-2021_01_07_425723 947 126 mail mail NN 10_1101-2021_01_07_425723 947 127 : : : 10_1101-2021_01_07_425723 947 128 j.t.pronk@tudelft.nl j.t.pronk@tudelft.nl UH 10_1101-2021_01_07_425723 947 129 , , , 10_1101-2021_01_07_425723 947 130 Tel tel NN 10_1101-2021_01_07_425723 947 131 : : : 10_1101-2021_01_07_425723 947 132 +31 +31 CD 10_1101-2021_01_07_425723 947 133 15 15 CD 10_1101-2021_01_07_425723 947 134 2783214 2783214 CD 10_1101-2021_01_07_425723 947 135 . . . 10_1101-2021_01_07_425723 948 1 941 941 CD 10_1101-2021_01_07_425723 948 2 .CC .CC : 10_1101-2021_01_07_425723 948 3 - - HYPH 10_1101-2021_01_07_425723 948 4 BY by IN 10_1101-2021_01_07_425723 948 5 - - HYPH 10_1101-2021_01_07_425723 948 6 NC NC NNP 10_1101-2021_01_07_425723 948 7 - - HYPH 10_1101-2021_01_07_425723 948 8 ND ND NNP 10_1101-2021_01_07_425723 948 9 4.0 4.0 CD 10_1101-2021_01_07_425723 948 10 International International NNP 10_1101-2021_01_07_425723 948 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 948 12 under under IN 10_1101-2021_01_07_425723 948 13 a a DT 10_1101-2021_01_07_425723 948 14 ( ( -LRB- 10_1101-2021_01_07_425723 948 15 which which WDT 10_1101-2021_01_07_425723 948 16 was be VBD 10_1101-2021_01_07_425723 948 17 not not RB 10_1101-2021_01_07_425723 948 18 certified certify VBN 10_1101-2021_01_07_425723 948 19 by by IN 10_1101-2021_01_07_425723 948 20 peer peer NN 10_1101-2021_01_07_425723 948 21 review review NN 10_1101-2021_01_07_425723 948 22 ) ) -RRB- 10_1101-2021_01_07_425723 948 23 is be VBZ 10_1101-2021_01_07_425723 948 24 the the DT 10_1101-2021_01_07_425723 948 25 author author NN 10_1101-2021_01_07_425723 948 26 / / SYM 10_1101-2021_01_07_425723 948 27 funder funder NN 10_1101-2021_01_07_425723 948 28 , , , 10_1101-2021_01_07_425723 948 29 who who WP 10_1101-2021_01_07_425723 948 30 has have VBZ 10_1101-2021_01_07_425723 948 31 granted grant VBN 10_1101-2021_01_07_425723 948 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 948 33 a a DT 10_1101-2021_01_07_425723 948 34 license license NN 10_1101-2021_01_07_425723 948 35 to to TO 10_1101-2021_01_07_425723 948 36 display display VB 10_1101-2021_01_07_425723 948 37 the the DT 10_1101-2021_01_07_425723 948 38 preprint preprint NN 10_1101-2021_01_07_425723 948 39 in in IN 10_1101-2021_01_07_425723 948 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 948 41 . . . 10_1101-2021_01_07_425723 949 1 It -PRON- PRP 10_1101-2021_01_07_425723 949 2 is be VBZ 10_1101-2021_01_07_425723 949 3 made make VBN 10_1101-2021_01_07_425723 949 4 The the DT 10_1101-2021_01_07_425723 949 5 copyright copyright NN 10_1101-2021_01_07_425723 949 6 holder holder NN 10_1101-2021_01_07_425723 949 7 for for IN 10_1101-2021_01_07_425723 949 8 this this DT 10_1101-2021_01_07_425723 949 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 949 10 version version NN 10_1101-2021_01_07_425723 949 11 posted post VBD 10_1101-2021_01_07_425723 949 12 January January NNP 10_1101-2021_01_07_425723 949 13 8 8 CD 10_1101-2021_01_07_425723 949 14 , , , 10_1101-2021_01_07_425723 949 15 2021 2021 CD 10_1101-2021_01_07_425723 949 16 . . . 10_1101-2021_01_07_425723 949 17 ; ; : 10_1101-2021_01_07_425723 949 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 949 19 : : : 10_1101-2021_01_07_425723 949 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 949 21 preprint preprint NN 10_1101-2021_01_07_425723 949 22 mailto:j.t.pronk@tudelft.nl mailto:j.t.pronk@tudelft.nl NN 10_1101-2021_01_07_425723 949 23 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 NNP 10_1101-2021_01_07_425723 949 24 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 949 25 51 51 CD 10_1101-2021_01_07_425723 949 26 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 949 27 Fig Fig NNP 10_1101-2021_01_07_425723 949 28 . . . 10_1101-2021_01_07_425723 950 1 1 1 CD 10_1101-2021_01_07_425723 950 2 | | NNP 10_1101-2021_01_07_425723 950 3 Ethanol Ethanol NNP 10_1101-2021_01_07_425723 950 4 evaporation evaporation NN 10_1101-2021_01_07_425723 950 5 rate rate NN 10_1101-2021_01_07_425723 950 6 . . . 10_1101-2021_01_07_425723 951 1 Ethanol ethanol NN 10_1101-2021_01_07_425723 951 2 concentration concentration NN 10_1101-2021_01_07_425723 951 3 over over IN 10_1101-2021_01_07_425723 951 4 time time NN 10_1101-2021_01_07_425723 951 5 with with IN 10_1101-2021_01_07_425723 951 6 reactor reactor NN 10_1101-2021_01_07_425723 951 7 volume volume NN 10_1101-2021_01_07_425723 951 8 942 942 CD 10_1101-2021_01_07_425723 951 9 of of IN 10_1101-2021_01_07_425723 951 10 1200 1200 CD 10_1101-2021_01_07_425723 951 11 mL mL NNP 10_1101-2021_01_07_425723 951 12 SM SM NNP 10_1101-2021_01_07_425723 951 13 glucose glucose NNP 10_1101-2021_01_07_425723 951 14 urea urea NNP 10_1101-2021_01_07_425723 951 15 media medium NNS 10_1101-2021_01_07_425723 951 16 maintained maintain VBD 10_1101-2021_01_07_425723 951 17 at at IN 10_1101-2021_01_07_425723 951 18 30 30 CD 10_1101-2021_01_07_425723 951 19 ° ° , 10_1101-2021_01_07_425723 951 20 C C NNP 10_1101-2021_01_07_425723 951 21 , , , 10_1101-2021_01_07_425723 951 22 stirred stir VBN 10_1101-2021_01_07_425723 951 23 with with IN 10_1101-2021_01_07_425723 951 24 800 800 CD 10_1101-2021_01_07_425723 951 25 rpm rpm NNS 10_1101-2021_01_07_425723 951 26 and and CC 10_1101-2021_01_07_425723 951 27 aerated aerate VBD 10_1101-2021_01_07_425723 951 28 with with IN 10_1101-2021_01_07_425723 951 29 a a DT 10_1101-2021_01_07_425723 951 30 943 943 CD 10_1101-2021_01_07_425723 951 31 volumetric volumetric NN 10_1101-2021_01_07_425723 951 32 gas gas NN 10_1101-2021_01_07_425723 951 33 flow flow NN 10_1101-2021_01_07_425723 951 34 rate rate NN 10_1101-2021_01_07_425723 951 35 of of IN 10_1101-2021_01_07_425723 951 36 500 500 CD 10_1101-2021_01_07_425723 951 37 mL·min-1 mL·min-1 NNS 10_1101-2021_01_07_425723 951 38 . . . 10_1101-2021_01_07_425723 952 1 The the DT 10_1101-2021_01_07_425723 952 2 reactor reactor NN 10_1101-2021_01_07_425723 952 3 off off IN 10_1101-2021_01_07_425723 952 4 - - HYPH 10_1101-2021_01_07_425723 952 5 gas gas NN 10_1101-2021_01_07_425723 952 6 was be VBD 10_1101-2021_01_07_425723 952 7 cooled cool VBN 10_1101-2021_01_07_425723 952 8 by by IN 10_1101-2021_01_07_425723 952 9 passing pass VBG 10_1101-2021_01_07_425723 952 10 through through IN 10_1101-2021_01_07_425723 952 11 a a DT 10_1101-2021_01_07_425723 952 12 condenser condenser NN 10_1101-2021_01_07_425723 952 13 944 944 CD 10_1101-2021_01_07_425723 952 14 cooled cool VBD 10_1101-2021_01_07_425723 952 15 at at IN 10_1101-2021_01_07_425723 952 16 2 2 CD 10_1101-2021_01_07_425723 952 17 ° ° , 10_1101-2021_01_07_425723 952 18 C C NNP 10_1101-2021_01_07_425723 952 19 . . . 10_1101-2021_01_07_425723 953 1 Circles circle NNS 10_1101-2021_01_07_425723 953 2 and and CC 10_1101-2021_01_07_425723 953 3 orange orange JJ 10_1101-2021_01_07_425723 953 4 line line NN 10_1101-2021_01_07_425723 953 5 represent represent VBP 10_1101-2021_01_07_425723 953 6 the the DT 10_1101-2021_01_07_425723 953 7 condition condition NN 10_1101-2021_01_07_425723 953 8 with with IN 10_1101-2021_01_07_425723 953 9 sparge sparge NN 10_1101-2021_01_07_425723 953 10 aeration aeration NN 10_1101-2021_01_07_425723 953 11 and and CC 10_1101-2021_01_07_425723 953 12 Tween Tween NNP 10_1101-2021_01_07_425723 953 13 80 80 CD 10_1101-2021_01_07_425723 953 14 ( ( -LRB- 10_1101-2021_01_07_425723 953 15 T t NN 10_1101-2021_01_07_425723 953 16 ) ) -RRB- 10_1101-2021_01_07_425723 953 17 945 945 CD 10_1101-2021_01_07_425723 953 18 media medium NNS 10_1101-2021_01_07_425723 953 19 supplementation supplementation NN 10_1101-2021_01_07_425723 953 20 , , , 10_1101-2021_01_07_425723 953 21 diamonds diamond NNS 10_1101-2021_01_07_425723 953 22 and and CC 10_1101-2021_01_07_425723 953 23 blue blue JJ 10_1101-2021_01_07_425723 953 24 line line NN 10_1101-2021_01_07_425723 953 25 head head NN 10_1101-2021_01_07_425723 953 26 - - HYPH 10_1101-2021_01_07_425723 953 27 space space NN 10_1101-2021_01_07_425723 953 28 aeration aeration NN 10_1101-2021_01_07_425723 953 29 with with IN 10_1101-2021_01_07_425723 953 30 Tween Tween NNP 10_1101-2021_01_07_425723 953 31 80 80 CD 10_1101-2021_01_07_425723 953 32 , , , 10_1101-2021_01_07_425723 953 33 triangle triangle NN 10_1101-2021_01_07_425723 953 34 and and CC 10_1101-2021_01_07_425723 953 35 red red JJ 10_1101-2021_01_07_425723 953 36 946 946 CD 10_1101-2021_01_07_425723 953 37 line line NN 10_1101-2021_01_07_425723 953 38 represent represent VBP 10_1101-2021_01_07_425723 953 39 head head NN 10_1101-2021_01_07_425723 953 40 space space NN 10_1101-2021_01_07_425723 953 41 aeration aeration NN 10_1101-2021_01_07_425723 953 42 and and CC 10_1101-2021_01_07_425723 953 43 Tween Tween NNP 10_1101-2021_01_07_425723 953 44 80 80 CD 10_1101-2021_01_07_425723 953 45 omission omission NN 10_1101-2021_01_07_425723 953 46 . . . 10_1101-2021_01_07_425723 954 1 Data datum NNS 10_1101-2021_01_07_425723 954 2 represent represent VBP 10_1101-2021_01_07_425723 954 3 mean mean VBP 10_1101-2021_01_07_425723 954 4 with with IN 10_1101-2021_01_07_425723 954 5 standard standard JJ 10_1101-2021_01_07_425723 954 6 947 947 CD 10_1101-2021_01_07_425723 954 7 deviation deviation NN 10_1101-2021_01_07_425723 954 8 from from IN 10_1101-2021_01_07_425723 954 9 three three CD 10_1101-2021_01_07_425723 954 10 independent independent JJ 10_1101-2021_01_07_425723 954 11 reactor reactor NN 10_1101-2021_01_07_425723 954 12 experiments experiment NNS 10_1101-2021_01_07_425723 954 13 . . . 10_1101-2021_01_07_425723 955 1 948 948 CD 10_1101-2021_01_07_425723 955 2 AGF AGF NNP 10_1101-2021_01_07_425723 955 3 Aeration Aeration NNP 10_1101-2021_01_07_425723 955 4 type type NN 10_1101-2021_01_07_425723 955 5 Ethanol ethanol NN 10_1101-2021_01_07_425723 955 6 evaporation evaporation NN 10_1101-2021_01_07_425723 955 7 ( ( -LRB- 10_1101-2021_01_07_425723 955 8 mmol·h-1 mmol·h-1 NNP 10_1101-2021_01_07_425723 955 9 ) ) -RRB- 10_1101-2021_01_07_425723 955 10 T T NNP 10_1101-2021_01_07_425723 955 11 Sparge sparge NN 10_1101-2021_01_07_425723 955 12 0.00578 0.00578 CD 10_1101-2021_01_07_425723 955 13 ± ± CD 10_1101-2021_01_07_425723 955 14 0.00062 0.00062 CD 10_1101-2021_01_07_425723 955 15 T T NNP 10_1101-2021_01_07_425723 955 16 Head head NN 10_1101-2021_01_07_425723 955 17 - - HYPH 10_1101-2021_01_07_425723 955 18 space space NN 10_1101-2021_01_07_425723 955 19 0.00625 0.00625 CD 10_1101-2021_01_07_425723 955 20 ± ± CD 10_1101-2021_01_07_425723 955 21 0.00032 0.00032 CD 10_1101-2021_01_07_425723 955 22 Head head NN 10_1101-2021_01_07_425723 955 23 - - HYPH 10_1101-2021_01_07_425723 955 24 space space NN 10_1101-2021_01_07_425723 955 25 0.00653 0.00653 CD 10_1101-2021_01_07_425723 955 26 ± ± CD 10_1101-2021_01_07_425723 955 27 0.00020 0.00020 CD 10_1101-2021_01_07_425723 955 28 949 949 CD 10_1101-2021_01_07_425723 955 29 950 950 CD 10_1101-2021_01_07_425723 955 30 100 100 CD 10_1101-2021_01_07_425723 955 31 150 150 CD 10_1101-2021_01_07_425723 955 32 200 200 CD 10_1101-2021_01_07_425723 955 33 250 250 CD 10_1101-2021_01_07_425723 955 34 300 300 CD 10_1101-2021_01_07_425723 955 35 350 350 CD 10_1101-2021_01_07_425723 955 36 400 400 CD 10_1101-2021_01_07_425723 955 37 450 450 CD 10_1101-2021_01_07_425723 955 38 0 0 CD 10_1101-2021_01_07_425723 955 39 24 24 CD 10_1101-2021_01_07_425723 955 40 48 48 CD 10_1101-2021_01_07_425723 955 41 72 72 CD 10_1101-2021_01_07_425723 955 42 96 96 CD 10_1101-2021_01_07_425723 955 43 120 120 CD 10_1101-2021_01_07_425723 955 44 144 144 CD 10_1101-2021_01_07_425723 955 45 168 168 CD 10_1101-2021_01_07_425723 955 46 192 192 CD 10_1101-2021_01_07_425723 955 47 c c NN 10_1101-2021_01_07_425723 955 48 e e NN 10_1101-2021_01_07_425723 955 49 th th XX 10_1101-2021_01_07_425723 955 50 an an DT 10_1101-2021_01_07_425723 955 51 ol old FW 10_1101-2021_01_07_425723 955 52 ( ( -LRB- 10_1101-2021_01_07_425723 955 53 m m NNP 10_1101-2021_01_07_425723 955 54 M M NNP 10_1101-2021_01_07_425723 955 55 ) ) -RRB- 10_1101-2021_01_07_425723 955 56 Time Time NNP 10_1101-2021_01_07_425723 955 57 ( ( -LRB- 10_1101-2021_01_07_425723 955 58 h h NNP 10_1101-2021_01_07_425723 955 59 ) ) -RRB- 10_1101-2021_01_07_425723 955 60 .CC .CC NFP 10_1101-2021_01_07_425723 955 61 - - : 10_1101-2021_01_07_425723 955 62 BY by IN 10_1101-2021_01_07_425723 955 63 - - HYPH 10_1101-2021_01_07_425723 955 64 NC NC NNP 10_1101-2021_01_07_425723 955 65 - - HYPH 10_1101-2021_01_07_425723 955 66 ND ND NNP 10_1101-2021_01_07_425723 955 67 4.0 4.0 CD 10_1101-2021_01_07_425723 955 68 International International NNP 10_1101-2021_01_07_425723 955 69 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 955 70 under under IN 10_1101-2021_01_07_425723 955 71 a a DT 10_1101-2021_01_07_425723 955 72 ( ( -LRB- 10_1101-2021_01_07_425723 955 73 which which WDT 10_1101-2021_01_07_425723 955 74 was be VBD 10_1101-2021_01_07_425723 955 75 not not RB 10_1101-2021_01_07_425723 955 76 certified certify VBN 10_1101-2021_01_07_425723 955 77 by by IN 10_1101-2021_01_07_425723 955 78 peer peer NN 10_1101-2021_01_07_425723 955 79 review review NN 10_1101-2021_01_07_425723 955 80 ) ) -RRB- 10_1101-2021_01_07_425723 955 81 is be VBZ 10_1101-2021_01_07_425723 955 82 the the DT 10_1101-2021_01_07_425723 955 83 author author NN 10_1101-2021_01_07_425723 955 84 / / SYM 10_1101-2021_01_07_425723 955 85 funder funder NN 10_1101-2021_01_07_425723 955 86 , , , 10_1101-2021_01_07_425723 955 87 who who WP 10_1101-2021_01_07_425723 955 88 has have VBZ 10_1101-2021_01_07_425723 955 89 granted grant VBN 10_1101-2021_01_07_425723 955 90 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 955 91 a a DT 10_1101-2021_01_07_425723 955 92 license license NN 10_1101-2021_01_07_425723 955 93 to to TO 10_1101-2021_01_07_425723 955 94 display display VB 10_1101-2021_01_07_425723 955 95 the the DT 10_1101-2021_01_07_425723 955 96 preprint preprint NN 10_1101-2021_01_07_425723 955 97 in in IN 10_1101-2021_01_07_425723 955 98 perpetuity perpetuity NN 10_1101-2021_01_07_425723 955 99 . . . 10_1101-2021_01_07_425723 956 1 It -PRON- PRP 10_1101-2021_01_07_425723 956 2 is be VBZ 10_1101-2021_01_07_425723 956 3 made make VBN 10_1101-2021_01_07_425723 956 4 The the DT 10_1101-2021_01_07_425723 956 5 copyright copyright NN 10_1101-2021_01_07_425723 956 6 holder holder NN 10_1101-2021_01_07_425723 956 7 for for IN 10_1101-2021_01_07_425723 956 8 this this DT 10_1101-2021_01_07_425723 956 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 956 10 version version NN 10_1101-2021_01_07_425723 956 11 posted post VBD 10_1101-2021_01_07_425723 956 12 January January NNP 10_1101-2021_01_07_425723 956 13 8 8 CD 10_1101-2021_01_07_425723 956 14 , , , 10_1101-2021_01_07_425723 956 15 2021 2021 CD 10_1101-2021_01_07_425723 956 16 . . . 10_1101-2021_01_07_425723 956 17 ; ; : 10_1101-2021_01_07_425723 956 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 956 19 : : : 10_1101-2021_01_07_425723 956 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 956 21 preprint preprint NN 10_1101-2021_01_07_425723 956 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 956 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 956 24 52 52 CD 10_1101-2021_01_07_425723 956 25 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 956 26 Fig Fig NNP 10_1101-2021_01_07_425723 956 27 . . . 10_1101-2021_01_07_425723 957 1 2 2 CD 10_1101-2021_01_07_425723 957 2 | | NNP 10_1101-2021_01_07_425723 957 3 Consensus Consensus NNP 10_1101-2021_01_07_425723 957 4 biological biological JJ 10_1101-2021_01_07_425723 957 5 process process NN 10_1101-2021_01_07_425723 957 6 GO GO NNP 10_1101-2021_01_07_425723 957 7 term term NN 10_1101-2021_01_07_425723 957 8 enrichment enrichment NN 10_1101-2021_01_07_425723 957 9 for for IN 10_1101-2021_01_07_425723 957 10 K. K. NNP 10_1101-2021_01_07_425723 957 11 marxianus marxianus NNP 10_1101-2021_01_07_425723 957 12 contrast contrast NNP 10_1101-2021_01_07_425723 957 13 951 951 CD 10_1101-2021_01_07_425723 957 14 31 31 CD 10_1101-2021_01_07_425723 957 15 . . . 10_1101-2021_01_07_425723 958 1 GO GO NNP 10_1101-2021_01_07_425723 958 2 terms term NNS 10_1101-2021_01_07_425723 958 3 are be VBP 10_1101-2021_01_07_425723 958 4 clustered cluster VBN 10_1101-2021_01_07_425723 958 5 according accord VBG 10_1101-2021_01_07_425723 958 6 to to IN 10_1101-2021_01_07_425723 958 7 their -PRON- PRP$ 10_1101-2021_01_07_425723 958 8 rank rank NN 10_1101-2021_01_07_425723 958 9 . . . 10_1101-2021_01_07_425723 959 1 See see VB 10_1101-2021_01_07_425723 959 2 legend legend NN 10_1101-2021_01_07_425723 959 3 of of IN 10_1101-2021_01_07_425723 959 4 Fig Fig NNP 10_1101-2021_01_07_425723 959 5 . . . 10_1101-2021_01_07_425723 960 1 2 2 CD 10_1101-2021_01_07_425723 960 2 for for IN 10_1101-2021_01_07_425723 960 3 experimental experimental JJ 10_1101-2021_01_07_425723 960 4 details detail NNS 10_1101-2021_01_07_425723 960 5 . . . 10_1101-2021_01_07_425723 961 1 952 952 CD 10_1101-2021_01_07_425723 961 2 .CC .CC : 10_1101-2021_01_07_425723 961 3 - - HYPH 10_1101-2021_01_07_425723 961 4 BY by IN 10_1101-2021_01_07_425723 961 5 - - HYPH 10_1101-2021_01_07_425723 961 6 NC NC NNP 10_1101-2021_01_07_425723 961 7 - - HYPH 10_1101-2021_01_07_425723 961 8 ND ND NNP 10_1101-2021_01_07_425723 961 9 4.0 4.0 CD 10_1101-2021_01_07_425723 961 10 International International NNP 10_1101-2021_01_07_425723 961 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 961 12 under under IN 10_1101-2021_01_07_425723 961 13 a a DT 10_1101-2021_01_07_425723 961 14 ( ( -LRB- 10_1101-2021_01_07_425723 961 15 which which WDT 10_1101-2021_01_07_425723 961 16 was be VBD 10_1101-2021_01_07_425723 961 17 not not RB 10_1101-2021_01_07_425723 961 18 certified certify VBN 10_1101-2021_01_07_425723 961 19 by by IN 10_1101-2021_01_07_425723 961 20 peer peer NN 10_1101-2021_01_07_425723 961 21 review review NN 10_1101-2021_01_07_425723 961 22 ) ) -RRB- 10_1101-2021_01_07_425723 961 23 is be VBZ 10_1101-2021_01_07_425723 961 24 the the DT 10_1101-2021_01_07_425723 961 25 author author NN 10_1101-2021_01_07_425723 961 26 / / SYM 10_1101-2021_01_07_425723 961 27 funder funder NN 10_1101-2021_01_07_425723 961 28 , , , 10_1101-2021_01_07_425723 961 29 who who WP 10_1101-2021_01_07_425723 961 30 has have VBZ 10_1101-2021_01_07_425723 961 31 granted grant VBN 10_1101-2021_01_07_425723 961 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 961 33 a a DT 10_1101-2021_01_07_425723 961 34 license license NN 10_1101-2021_01_07_425723 961 35 to to TO 10_1101-2021_01_07_425723 961 36 display display VB 10_1101-2021_01_07_425723 961 37 the the DT 10_1101-2021_01_07_425723 961 38 preprint preprint NN 10_1101-2021_01_07_425723 961 39 in in IN 10_1101-2021_01_07_425723 961 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 961 41 . . . 10_1101-2021_01_07_425723 962 1 It -PRON- PRP 10_1101-2021_01_07_425723 962 2 is be VBZ 10_1101-2021_01_07_425723 962 3 made make VBN 10_1101-2021_01_07_425723 962 4 The the DT 10_1101-2021_01_07_425723 962 5 copyright copyright NN 10_1101-2021_01_07_425723 962 6 holder holder NN 10_1101-2021_01_07_425723 962 7 for for IN 10_1101-2021_01_07_425723 962 8 this this DT 10_1101-2021_01_07_425723 962 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 962 10 version version NN 10_1101-2021_01_07_425723 962 11 posted post VBD 10_1101-2021_01_07_425723 962 12 January January NNP 10_1101-2021_01_07_425723 962 13 8 8 CD 10_1101-2021_01_07_425723 962 14 , , , 10_1101-2021_01_07_425723 962 15 2021 2021 CD 10_1101-2021_01_07_425723 962 16 . . . 10_1101-2021_01_07_425723 962 17 ; ; : 10_1101-2021_01_07_425723 962 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 962 19 : : : 10_1101-2021_01_07_425723 962 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 962 21 preprint preprint NN 10_1101-2021_01_07_425723 962 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 962 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 962 24 53 53 CD 10_1101-2021_01_07_425723 962 25 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 962 26 Fig Fig NNP 10_1101-2021_01_07_425723 962 27 . . . 10_1101-2021_01_07_425723 963 1 3 3 CD 10_1101-2021_01_07_425723 963 2 | | NNP 10_1101-2021_01_07_425723 963 3 Consensus Consensus NNP 10_1101-2021_01_07_425723 963 4 biological biological JJ 10_1101-2021_01_07_425723 963 5 process process NN 10_1101-2021_01_07_425723 963 6 GO GO NNP 10_1101-2021_01_07_425723 963 7 term term NN 10_1101-2021_01_07_425723 963 8 enrichment enrichment NN 10_1101-2021_01_07_425723 963 9 for for IN 10_1101-2021_01_07_425723 963 10 K. K. NNP 10_1101-2021_01_07_425723 963 11 marxianus marxianus NNP 10_1101-2021_01_07_425723 963 12 contrast contrast NN 10_1101-2021_01_07_425723 963 13 953 953 CD 10_1101-2021_01_07_425723 963 14 43 43 CD 10_1101-2021_01_07_425723 963 15 . . . 10_1101-2021_01_07_425723 964 1 GO GO NNP 10_1101-2021_01_07_425723 964 2 terms term NNS 10_1101-2021_01_07_425723 964 3 are be VBP 10_1101-2021_01_07_425723 964 4 clustered cluster VBN 10_1101-2021_01_07_425723 964 5 according accord VBG 10_1101-2021_01_07_425723 964 6 to to IN 10_1101-2021_01_07_425723 964 7 their -PRON- PRP$ 10_1101-2021_01_07_425723 964 8 rank rank NN 10_1101-2021_01_07_425723 964 9 . . . 10_1101-2021_01_07_425723 965 1 See see VB 10_1101-2021_01_07_425723 965 2 legend legend NN 10_1101-2021_01_07_425723 965 3 of of IN 10_1101-2021_01_07_425723 965 4 Fig Fig NNP 10_1101-2021_01_07_425723 965 5 . . . 10_1101-2021_01_07_425723 966 1 2 2 CD 10_1101-2021_01_07_425723 966 2 for for IN 10_1101-2021_01_07_425723 966 3 experimental experimental JJ 10_1101-2021_01_07_425723 966 4 details detail NNS 10_1101-2021_01_07_425723 966 5 . . . 10_1101-2021_01_07_425723 967 1 954 954 CD 10_1101-2021_01_07_425723 967 2 .CC .CC : 10_1101-2021_01_07_425723 967 3 - - HYPH 10_1101-2021_01_07_425723 967 4 BY by IN 10_1101-2021_01_07_425723 967 5 - - HYPH 10_1101-2021_01_07_425723 967 6 NC NC NNP 10_1101-2021_01_07_425723 967 7 - - HYPH 10_1101-2021_01_07_425723 967 8 ND ND NNP 10_1101-2021_01_07_425723 967 9 4.0 4.0 CD 10_1101-2021_01_07_425723 967 10 International International NNP 10_1101-2021_01_07_425723 967 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 967 12 under under IN 10_1101-2021_01_07_425723 967 13 a a DT 10_1101-2021_01_07_425723 967 14 ( ( -LRB- 10_1101-2021_01_07_425723 967 15 which which WDT 10_1101-2021_01_07_425723 967 16 was be VBD 10_1101-2021_01_07_425723 967 17 not not RB 10_1101-2021_01_07_425723 967 18 certified certify VBN 10_1101-2021_01_07_425723 967 19 by by IN 10_1101-2021_01_07_425723 967 20 peer peer NN 10_1101-2021_01_07_425723 967 21 review review NN 10_1101-2021_01_07_425723 967 22 ) ) -RRB- 10_1101-2021_01_07_425723 967 23 is be VBZ 10_1101-2021_01_07_425723 967 24 the the DT 10_1101-2021_01_07_425723 967 25 author author NN 10_1101-2021_01_07_425723 967 26 / / SYM 10_1101-2021_01_07_425723 967 27 funder funder NN 10_1101-2021_01_07_425723 967 28 , , , 10_1101-2021_01_07_425723 967 29 who who WP 10_1101-2021_01_07_425723 967 30 has have VBZ 10_1101-2021_01_07_425723 967 31 granted grant VBN 10_1101-2021_01_07_425723 967 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 967 33 a a DT 10_1101-2021_01_07_425723 967 34 license license NN 10_1101-2021_01_07_425723 967 35 to to TO 10_1101-2021_01_07_425723 967 36 display display VB 10_1101-2021_01_07_425723 967 37 the the DT 10_1101-2021_01_07_425723 967 38 preprint preprint NN 10_1101-2021_01_07_425723 967 39 in in IN 10_1101-2021_01_07_425723 967 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 967 41 . . . 10_1101-2021_01_07_425723 968 1 It -PRON- PRP 10_1101-2021_01_07_425723 968 2 is be VBZ 10_1101-2021_01_07_425723 968 3 made make VBN 10_1101-2021_01_07_425723 968 4 The the DT 10_1101-2021_01_07_425723 968 5 copyright copyright NN 10_1101-2021_01_07_425723 968 6 holder holder NN 10_1101-2021_01_07_425723 968 7 for for IN 10_1101-2021_01_07_425723 968 8 this this DT 10_1101-2021_01_07_425723 968 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 968 10 version version NN 10_1101-2021_01_07_425723 968 11 posted post VBD 10_1101-2021_01_07_425723 968 12 January January NNP 10_1101-2021_01_07_425723 968 13 8 8 CD 10_1101-2021_01_07_425723 968 14 , , , 10_1101-2021_01_07_425723 968 15 2021 2021 CD 10_1101-2021_01_07_425723 968 16 . . . 10_1101-2021_01_07_425723 968 17 ; ; : 10_1101-2021_01_07_425723 968 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 968 19 : : : 10_1101-2021_01_07_425723 968 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 968 21 preprint preprint NN 10_1101-2021_01_07_425723 968 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 968 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 968 24 54 54 CD 10_1101-2021_01_07_425723 968 25 955 955 CD 10_1101-2021_01_07_425723 968 26 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 968 27 Fig Fig NNP 10_1101-2021_01_07_425723 968 28 . . . 10_1101-2021_01_07_425723 969 1 4 4 CD 10_1101-2021_01_07_425723 969 2 | | NNP 10_1101-2021_01_07_425723 969 3 Consensus Consensus NNP 10_1101-2021_01_07_425723 969 4 biological biological JJ 10_1101-2021_01_07_425723 969 5 process process NN 10_1101-2021_01_07_425723 969 6 GO GO NNP 10_1101-2021_01_07_425723 969 7 term term NN 10_1101-2021_01_07_425723 969 8 enrichment enrichment NN 10_1101-2021_01_07_425723 969 9 for for IN 10_1101-2021_01_07_425723 969 10 S. S. NNP 10_1101-2021_01_07_425723 969 11 cerevisiae cerevisiae NNP 10_1101-2021_01_07_425723 969 12 contrast contrast NN 10_1101-2021_01_07_425723 969 13 31 31 CD 10_1101-2021_01_07_425723 969 14 . . . 10_1101-2021_01_07_425723 970 1 956 956 CD 10_1101-2021_01_07_425723 970 2 GO GO NNP 10_1101-2021_01_07_425723 970 3 terms term NNS 10_1101-2021_01_07_425723 970 4 are be VBP 10_1101-2021_01_07_425723 970 5 clustered cluster VBN 10_1101-2021_01_07_425723 970 6 according accord VBG 10_1101-2021_01_07_425723 970 7 to to IN 10_1101-2021_01_07_425723 970 8 their -PRON- PRP$ 10_1101-2021_01_07_425723 970 9 rank rank NN 10_1101-2021_01_07_425723 970 10 . . . 10_1101-2021_01_07_425723 971 1 See see VB 10_1101-2021_01_07_425723 971 2 legend legend NN 10_1101-2021_01_07_425723 971 3 of of IN 10_1101-2021_01_07_425723 971 4 Fig Fig NNP 10_1101-2021_01_07_425723 971 5 . . . 10_1101-2021_01_07_425723 972 1 2 2 CD 10_1101-2021_01_07_425723 972 2 for for IN 10_1101-2021_01_07_425723 972 3 experimental experimental JJ 10_1101-2021_01_07_425723 972 4 details detail NNS 10_1101-2021_01_07_425723 972 5 . . . 10_1101-2021_01_07_425723 973 1 957 957 CD 10_1101-2021_01_07_425723 973 2 .CC .CC : 10_1101-2021_01_07_425723 973 3 - - HYPH 10_1101-2021_01_07_425723 973 4 BY by IN 10_1101-2021_01_07_425723 973 5 - - HYPH 10_1101-2021_01_07_425723 973 6 NC NC NNP 10_1101-2021_01_07_425723 973 7 - - HYPH 10_1101-2021_01_07_425723 973 8 ND ND NNP 10_1101-2021_01_07_425723 973 9 4.0 4.0 CD 10_1101-2021_01_07_425723 973 10 International International NNP 10_1101-2021_01_07_425723 973 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 973 12 under under IN 10_1101-2021_01_07_425723 973 13 a a DT 10_1101-2021_01_07_425723 973 14 ( ( -LRB- 10_1101-2021_01_07_425723 973 15 which which WDT 10_1101-2021_01_07_425723 973 16 was be VBD 10_1101-2021_01_07_425723 973 17 not not RB 10_1101-2021_01_07_425723 973 18 certified certify VBN 10_1101-2021_01_07_425723 973 19 by by IN 10_1101-2021_01_07_425723 973 20 peer peer NN 10_1101-2021_01_07_425723 973 21 review review NN 10_1101-2021_01_07_425723 973 22 ) ) -RRB- 10_1101-2021_01_07_425723 973 23 is be VBZ 10_1101-2021_01_07_425723 973 24 the the DT 10_1101-2021_01_07_425723 973 25 author author NN 10_1101-2021_01_07_425723 973 26 / / SYM 10_1101-2021_01_07_425723 973 27 funder funder NN 10_1101-2021_01_07_425723 973 28 , , , 10_1101-2021_01_07_425723 973 29 who who WP 10_1101-2021_01_07_425723 973 30 has have VBZ 10_1101-2021_01_07_425723 973 31 granted grant VBN 10_1101-2021_01_07_425723 973 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 973 33 a a DT 10_1101-2021_01_07_425723 973 34 license license NN 10_1101-2021_01_07_425723 973 35 to to TO 10_1101-2021_01_07_425723 973 36 display display VB 10_1101-2021_01_07_425723 973 37 the the DT 10_1101-2021_01_07_425723 973 38 preprint preprint NN 10_1101-2021_01_07_425723 973 39 in in IN 10_1101-2021_01_07_425723 973 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 973 41 . . . 10_1101-2021_01_07_425723 974 1 It -PRON- PRP 10_1101-2021_01_07_425723 974 2 is be VBZ 10_1101-2021_01_07_425723 974 3 made make VBN 10_1101-2021_01_07_425723 974 4 The the DT 10_1101-2021_01_07_425723 974 5 copyright copyright NN 10_1101-2021_01_07_425723 974 6 holder holder NN 10_1101-2021_01_07_425723 974 7 for for IN 10_1101-2021_01_07_425723 974 8 this this DT 10_1101-2021_01_07_425723 974 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 974 10 version version NN 10_1101-2021_01_07_425723 974 11 posted post VBD 10_1101-2021_01_07_425723 974 12 January January NNP 10_1101-2021_01_07_425723 974 13 8 8 CD 10_1101-2021_01_07_425723 974 14 , , , 10_1101-2021_01_07_425723 974 15 2021 2021 CD 10_1101-2021_01_07_425723 974 16 . . . 10_1101-2021_01_07_425723 974 17 ; ; : 10_1101-2021_01_07_425723 974 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 974 19 : : : 10_1101-2021_01_07_425723 974 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 974 21 preprint preprint NN 10_1101-2021_01_07_425723 974 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 974 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 974 24 55 55 CD 10_1101-2021_01_07_425723 974 25 958 958 CD 10_1101-2021_01_07_425723 974 26 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 974 27 Fig Fig NNP 10_1101-2021_01_07_425723 974 28 . . . 10_1101-2021_01_07_425723 975 1 5 5 CD 10_1101-2021_01_07_425723 975 2 | | NNP 10_1101-2021_01_07_425723 975 3 Consensus Consensus NNP 10_1101-2021_01_07_425723 975 4 biological biological JJ 10_1101-2021_01_07_425723 975 5 process process NN 10_1101-2021_01_07_425723 975 6 GO GO NNP 10_1101-2021_01_07_425723 975 7 term term NN 10_1101-2021_01_07_425723 975 8 enrichment enrichment NN 10_1101-2021_01_07_425723 975 9 for for IN 10_1101-2021_01_07_425723 975 10 S. S. NNP 10_1101-2021_01_07_425723 975 11 cerevisiae cerevisiae NNP 10_1101-2021_01_07_425723 975 12 contrast contrast NN 10_1101-2021_01_07_425723 975 13 43 43 CD 10_1101-2021_01_07_425723 975 14 . . . 10_1101-2021_01_07_425723 976 1 959 959 CD 10_1101-2021_01_07_425723 976 2 GO GO NNP 10_1101-2021_01_07_425723 976 3 terms term NNS 10_1101-2021_01_07_425723 976 4 are be VBP 10_1101-2021_01_07_425723 976 5 clustered cluster VBN 10_1101-2021_01_07_425723 976 6 according accord VBG 10_1101-2021_01_07_425723 976 7 to to IN 10_1101-2021_01_07_425723 976 8 their -PRON- PRP$ 10_1101-2021_01_07_425723 976 9 rank rank NN 10_1101-2021_01_07_425723 976 10 . . . 10_1101-2021_01_07_425723 977 1 See see VB 10_1101-2021_01_07_425723 977 2 legend legend NN 10_1101-2021_01_07_425723 977 3 of of IN 10_1101-2021_01_07_425723 977 4 Fig Fig NNP 10_1101-2021_01_07_425723 977 5 . . . 10_1101-2021_01_07_425723 978 1 2 2 CD 10_1101-2021_01_07_425723 978 2 for for IN 10_1101-2021_01_07_425723 978 3 experimental experimental JJ 10_1101-2021_01_07_425723 978 4 details detail NNS 10_1101-2021_01_07_425723 978 5 . . . 10_1101-2021_01_07_425723 979 1 960 960 CD 10_1101-2021_01_07_425723 979 2 .CC .CC : 10_1101-2021_01_07_425723 979 3 - - HYPH 10_1101-2021_01_07_425723 979 4 BY by IN 10_1101-2021_01_07_425723 979 5 - - HYPH 10_1101-2021_01_07_425723 979 6 NC NC NNP 10_1101-2021_01_07_425723 979 7 - - HYPH 10_1101-2021_01_07_425723 979 8 ND ND NNP 10_1101-2021_01_07_425723 979 9 4.0 4.0 CD 10_1101-2021_01_07_425723 979 10 International International NNP 10_1101-2021_01_07_425723 979 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 979 12 under under IN 10_1101-2021_01_07_425723 979 13 a a DT 10_1101-2021_01_07_425723 979 14 ( ( -LRB- 10_1101-2021_01_07_425723 979 15 which which WDT 10_1101-2021_01_07_425723 979 16 was be VBD 10_1101-2021_01_07_425723 979 17 not not RB 10_1101-2021_01_07_425723 979 18 certified certify VBN 10_1101-2021_01_07_425723 979 19 by by IN 10_1101-2021_01_07_425723 979 20 peer peer NN 10_1101-2021_01_07_425723 979 21 review review NN 10_1101-2021_01_07_425723 979 22 ) ) -RRB- 10_1101-2021_01_07_425723 979 23 is be VBZ 10_1101-2021_01_07_425723 979 24 the the DT 10_1101-2021_01_07_425723 979 25 author author NN 10_1101-2021_01_07_425723 979 26 / / SYM 10_1101-2021_01_07_425723 979 27 funder funder NN 10_1101-2021_01_07_425723 979 28 , , , 10_1101-2021_01_07_425723 979 29 who who WP 10_1101-2021_01_07_425723 979 30 has have VBZ 10_1101-2021_01_07_425723 979 31 granted grant VBN 10_1101-2021_01_07_425723 979 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 979 33 a a DT 10_1101-2021_01_07_425723 979 34 license license NN 10_1101-2021_01_07_425723 979 35 to to TO 10_1101-2021_01_07_425723 979 36 display display VB 10_1101-2021_01_07_425723 979 37 the the DT 10_1101-2021_01_07_425723 979 38 preprint preprint NN 10_1101-2021_01_07_425723 979 39 in in IN 10_1101-2021_01_07_425723 979 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 979 41 . . . 10_1101-2021_01_07_425723 980 1 It -PRON- PRP 10_1101-2021_01_07_425723 980 2 is be VBZ 10_1101-2021_01_07_425723 980 3 made make VBN 10_1101-2021_01_07_425723 980 4 The the DT 10_1101-2021_01_07_425723 980 5 copyright copyright NN 10_1101-2021_01_07_425723 980 6 holder holder NN 10_1101-2021_01_07_425723 980 7 for for IN 10_1101-2021_01_07_425723 980 8 this this DT 10_1101-2021_01_07_425723 980 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 980 10 version version NN 10_1101-2021_01_07_425723 980 11 posted post VBD 10_1101-2021_01_07_425723 980 12 January January NNP 10_1101-2021_01_07_425723 980 13 8 8 CD 10_1101-2021_01_07_425723 980 14 , , , 10_1101-2021_01_07_425723 980 15 2021 2021 CD 10_1101-2021_01_07_425723 980 16 . . . 10_1101-2021_01_07_425723 980 17 ; ; : 10_1101-2021_01_07_425723 980 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 980 19 : : : 10_1101-2021_01_07_425723 980 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 980 21 preprint preprint NN 10_1101-2021_01_07_425723 980 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 980 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 980 24 56 56 CD 10_1101-2021_01_07_425723 980 25 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 980 26 Fig Fig NNP 10_1101-2021_01_07_425723 980 27 . . . 10_1101-2021_01_07_425723 981 1 6 6 CD 10_1101-2021_01_07_425723 981 2 | | CD 10_1101-2021_01_07_425723 981 3 GO GO NNP 10_1101-2021_01_07_425723 981 4 term term NN 10_1101-2021_01_07_425723 981 5 enrichment enrichment NN 10_1101-2021_01_07_425723 981 6 comparison comparison NN 10_1101-2021_01_07_425723 981 7 of of IN 10_1101-2021_01_07_425723 981 8 biological biological JJ 10_1101-2021_01_07_425723 981 9 process process NN 10_1101-2021_01_07_425723 981 10 of of IN 10_1101-2021_01_07_425723 981 11 K. K. NNP 10_1101-2021_01_07_425723 981 12 marxianus marxianus NNP 10_1101-2021_01_07_425723 981 13 ( ( -LRB- 10_1101-2021_01_07_425723 981 14 kmar kmar NNP 10_1101-2021_01_07_425723 981 15 ) ) -RRB- 10_1101-2021_01_07_425723 981 16 961 961 CD 10_1101-2021_01_07_425723 981 17 to to IN 10_1101-2021_01_07_425723 981 18 S. S. NNP 10_1101-2021_01_07_425723 981 19 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 981 20 ( ( -LRB- 10_1101-2021_01_07_425723 981 21 scer scer NN 10_1101-2021_01_07_425723 981 22 ) ) -RRB- 10_1101-2021_01_07_425723 981 23 of of IN 10_1101-2021_01_07_425723 981 24 contrast contrast NN 10_1101-2021_01_07_425723 981 25 43 43 CD 10_1101-2021_01_07_425723 981 26 . . . 10_1101-2021_01_07_425723 982 1 GO GO NNP 10_1101-2021_01_07_425723 982 2 terms term NNS 10_1101-2021_01_07_425723 982 3 were be VBD 10_1101-2021_01_07_425723 982 4 annotated annotate VBN 10_1101-2021_01_07_425723 982 5 with with IN 10_1101-2021_01_07_425723 982 6 the the DT 10_1101-2021_01_07_425723 982 7 color color NN 10_1101-2021_01_07_425723 982 8 of of IN 10_1101-2021_01_07_425723 982 9 distinct distinct JJ 10_1101-2021_01_07_425723 982 10 directionality directionality NN 10_1101-2021_01_07_425723 982 11 962 962 CD 10_1101-2021_01_07_425723 982 12 ( ( -LRB- 10_1101-2021_01_07_425723 982 13 up up IN 10_1101-2021_01_07_425723 982 14 ( ( -LRB- 10_1101-2021_01_07_425723 982 15 blue blue NNP 10_1101-2021_01_07_425723 982 16 ) ) -RRB- 10_1101-2021_01_07_425723 982 17 down down IN 10_1101-2021_01_07_425723 982 18 ( ( -LRB- 10_1101-2021_01_07_425723 982 19 brown brown NNP 10_1101-2021_01_07_425723 982 20 ) ) -RRB- 10_1101-2021_01_07_425723 982 21 ) ) -RRB- 10_1101-2021_01_07_425723 982 22 and and CC 10_1101-2021_01_07_425723 982 23 the the DT 10_1101-2021_01_07_425723 982 24 color color NN 10_1101-2021_01_07_425723 982 25 intensity intensity NN 10_1101-2021_01_07_425723 982 26 was be VBD 10_1101-2021_01_07_425723 982 27 determined determine VBN 10_1101-2021_01_07_425723 982 28 by by IN 10_1101-2021_01_07_425723 982 29 the the DT 10_1101-2021_01_07_425723 982 30 magnitude magnitude NN 10_1101-2021_01_07_425723 982 31 of of IN 10_1101-2021_01_07_425723 982 32 the the DT 10_1101-2021_01_07_425723 982 33 inverse inverse NN 10_1101-2021_01_07_425723 982 34 rank rank NN 10_1101-2021_01_07_425723 982 35 . . . 10_1101-2021_01_07_425723 983 1 963 963 CD 10_1101-2021_01_07_425723 983 2 GO GO NNP 10_1101-2021_01_07_425723 983 3 terms term NNS 10_1101-2021_01_07_425723 983 4 with with IN 10_1101-2021_01_07_425723 983 5 significant significant JJ 10_1101-2021_01_07_425723 983 6 mixed mixed JJ 10_1101-2021_01_07_425723 983 7 - - HYPH 10_1101-2021_01_07_425723 983 8 directionality directionality NN 10_1101-2021_01_07_425723 983 9 or or CC 10_1101-2021_01_07_425723 983 10 non non NN 10_1101-2021_01_07_425723 983 11 - - JJ 10_1101-2021_01_07_425723 983 12 directionality directionality JJ 10_1101-2021_01_07_425723 983 13 , , , 10_1101-2021_01_07_425723 983 14 as as IN 10_1101-2021_01_07_425723 983 15 having have VBG 10_1101-2021_01_07_425723 983 16 no no DT 10_1101-2021_01_07_425723 983 17 pronounced pronounce VBN 10_1101-2021_01_07_425723 983 18 distinct distinct JJ 10_1101-2021_01_07_425723 983 19 964 964 CD 10_1101-2021_01_07_425723 983 20 directionality directionality NN 10_1101-2021_01_07_425723 983 21 , , , 10_1101-2021_01_07_425723 983 22 are be VBP 10_1101-2021_01_07_425723 983 23 colored color VBN 10_1101-2021_01_07_425723 983 24 white white JJ 10_1101-2021_01_07_425723 983 25 . . . 10_1101-2021_01_07_425723 984 1 Shared share VBN 10_1101-2021_01_07_425723 984 2 GO GO NNP 10_1101-2021_01_07_425723 984 3 terms term NNS 10_1101-2021_01_07_425723 984 4 between between IN 10_1101-2021_01_07_425723 984 5 K. K. NNP 10_1101-2021_01_07_425723 984 6 marxianus marxianus NN 10_1101-2021_01_07_425723 984 7 and and CC 10_1101-2021_01_07_425723 984 8 S. S. NNP 10_1101-2021_01_07_425723 984 9 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 984 10 are be VBP 10_1101-2021_01_07_425723 984 11 965 965 CD 10_1101-2021_01_07_425723 984 12 connected connect VBN 10_1101-2021_01_07_425723 984 13 by by IN 10_1101-2021_01_07_425723 984 14 a a DT 10_1101-2021_01_07_425723 984 15 line.966 line.966 ADD 10_1101-2021_01_07_425723 984 16 .CC .CC : 10_1101-2021_01_07_425723 984 17 - - HYPH 10_1101-2021_01_07_425723 984 18 BY by IN 10_1101-2021_01_07_425723 984 19 - - HYPH 10_1101-2021_01_07_425723 984 20 NC NC NNP 10_1101-2021_01_07_425723 984 21 - - HYPH 10_1101-2021_01_07_425723 984 22 ND ND NNP 10_1101-2021_01_07_425723 984 23 4.0 4.0 CD 10_1101-2021_01_07_425723 984 24 International International NNP 10_1101-2021_01_07_425723 984 25 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 984 26 under under IN 10_1101-2021_01_07_425723 984 27 a a DT 10_1101-2021_01_07_425723 984 28 ( ( -LRB- 10_1101-2021_01_07_425723 984 29 which which WDT 10_1101-2021_01_07_425723 984 30 was be VBD 10_1101-2021_01_07_425723 984 31 not not RB 10_1101-2021_01_07_425723 984 32 certified certify VBN 10_1101-2021_01_07_425723 984 33 by by IN 10_1101-2021_01_07_425723 984 34 peer peer NN 10_1101-2021_01_07_425723 984 35 review review NN 10_1101-2021_01_07_425723 984 36 ) ) -RRB- 10_1101-2021_01_07_425723 984 37 is be VBZ 10_1101-2021_01_07_425723 984 38 the the DT 10_1101-2021_01_07_425723 984 39 author author NN 10_1101-2021_01_07_425723 984 40 / / SYM 10_1101-2021_01_07_425723 984 41 funder funder NN 10_1101-2021_01_07_425723 984 42 , , , 10_1101-2021_01_07_425723 984 43 who who WP 10_1101-2021_01_07_425723 984 44 has have VBZ 10_1101-2021_01_07_425723 984 45 granted grant VBN 10_1101-2021_01_07_425723 984 46 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 984 47 a a DT 10_1101-2021_01_07_425723 984 48 license license NN 10_1101-2021_01_07_425723 984 49 to to TO 10_1101-2021_01_07_425723 984 50 display display VB 10_1101-2021_01_07_425723 984 51 the the DT 10_1101-2021_01_07_425723 984 52 preprint preprint NN 10_1101-2021_01_07_425723 984 53 in in IN 10_1101-2021_01_07_425723 984 54 perpetuity perpetuity NN 10_1101-2021_01_07_425723 984 55 . . . 10_1101-2021_01_07_425723 985 1 It -PRON- PRP 10_1101-2021_01_07_425723 985 2 is be VBZ 10_1101-2021_01_07_425723 985 3 made make VBN 10_1101-2021_01_07_425723 985 4 The the DT 10_1101-2021_01_07_425723 985 5 copyright copyright NN 10_1101-2021_01_07_425723 985 6 holder holder NN 10_1101-2021_01_07_425723 985 7 for for IN 10_1101-2021_01_07_425723 985 8 this this DT 10_1101-2021_01_07_425723 985 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 985 10 version version NN 10_1101-2021_01_07_425723 985 11 posted post VBD 10_1101-2021_01_07_425723 985 12 January January NNP 10_1101-2021_01_07_425723 985 13 8 8 CD 10_1101-2021_01_07_425723 985 14 , , , 10_1101-2021_01_07_425723 985 15 2021 2021 CD 10_1101-2021_01_07_425723 985 16 . . . 10_1101-2021_01_07_425723 985 17 ; ; : 10_1101-2021_01_07_425723 985 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 985 19 : : : 10_1101-2021_01_07_425723 985 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 985 21 preprint preprint NN 10_1101-2021_01_07_425723 985 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 985 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 985 24 57 57 CD 10_1101-2021_01_07_425723 985 25 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 985 26 Fig Fig NNP 10_1101-2021_01_07_425723 985 27 . . . 10_1101-2021_01_07_425723 986 1 7 7 CD 10_1101-2021_01_07_425723 986 2 | | NNP 10_1101-2021_01_07_425723 986 3 Uptake Uptake NNP 10_1101-2021_01_07_425723 986 4 of of IN 10_1101-2021_01_07_425723 986 5 the the DT 10_1101-2021_01_07_425723 986 6 fluorescent fluorescent NN 10_1101-2021_01_07_425723 986 7 sterol sterol NN 10_1101-2021_01_07_425723 986 8 derivative derivative JJ 10_1101-2021_01_07_425723 986 9 NBDC NBDC NNP 10_1101-2021_01_07_425723 986 10 by by IN 10_1101-2021_01_07_425723 986 11 S. S. NNP 10_1101-2021_01_07_425723 986 12 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 986 13 and and CC 10_1101-2021_01_07_425723 986 14 K. K. NNP 10_1101-2021_01_07_425723 986 15 967 967 CD 10_1101-2021_01_07_425723 986 16 marxianus marxianus NN 10_1101-2021_01_07_425723 986 17 strains strain VBZ 10_1101-2021_01_07_425723 986 18 after after IN 10_1101-2021_01_07_425723 986 19 23 23 CD 10_1101-2021_01_07_425723 986 20 h h NN 10_1101-2021_01_07_425723 986 21 staining staining NN 10_1101-2021_01_07_425723 986 22 . . . 10_1101-2021_01_07_425723 987 1 968 968 CD 10_1101-2021_01_07_425723 987 2 Flow Flow NNP 10_1101-2021_01_07_425723 987 3 cytometry cytometry NN 10_1101-2021_01_07_425723 987 4 data datum NNS 10_1101-2021_01_07_425723 987 5 of of IN 10_1101-2021_01_07_425723 987 6 Fig Fig NNP 10_1101-2021_01_07_425723 987 7 . . . 10_1101-2021_01_07_425723 988 1 4 4 CD 10_1101-2021_01_07_425723 988 2 with with IN 10_1101-2021_01_07_425723 988 3 prolonged prolong VBN 10_1101-2021_01_07_425723 988 4 staining staining NN 10_1101-2021_01_07_425723 988 5 after after IN 10_1101-2021_01_07_425723 988 6 pulse pulse NN 10_1101-2021_01_07_425723 988 7 - - HYPH 10_1101-2021_01_07_425723 988 8 addition addition NN 10_1101-2021_01_07_425723 988 9 of of IN 10_1101-2021_01_07_425723 988 10 NBD NBD NNP 10_1101-2021_01_07_425723 988 11 - - HYPH 10_1101-2021_01_07_425723 988 12 cholesterol cholesterol NN 10_1101-2021_01_07_425723 988 13 to to IN 10_1101-2021_01_07_425723 988 14 the the DT 10_1101-2021_01_07_425723 988 15 969 969 CD 10_1101-2021_01_07_425723 988 16 shake shake NN 10_1101-2021_01_07_425723 988 17 - - HYPH 10_1101-2021_01_07_425723 988 18 flask flask NN 10_1101-2021_01_07_425723 988 19 cultures culture NNS 10_1101-2021_01_07_425723 988 20 for for IN 10_1101-2021_01_07_425723 988 21 23 23 CD 10_1101-2021_01_07_425723 988 22 h. h. NNS 10_1101-2021_01_07_425723 988 23 Bar bar VB 10_1101-2021_01_07_425723 988 24 charts chart NNS 10_1101-2021_01_07_425723 988 25 of of IN 10_1101-2021_01_07_425723 988 26 the the DT 10_1101-2021_01_07_425723 988 27 median median JJ 10_1101-2021_01_07_425723 988 28 and and CC 10_1101-2021_01_07_425723 988 29 pooled pool VBN 10_1101-2021_01_07_425723 988 30 standard standard JJ 10_1101-2021_01_07_425723 988 31 deviation deviation NN 10_1101-2021_01_07_425723 988 32 of of IN 10_1101-2021_01_07_425723 988 33 the the DT 10_1101-2021_01_07_425723 988 34 NBD-970 NBD-970 NNP 10_1101-2021_01_07_425723 988 35 cholesterol cholesterol NN 10_1101-2021_01_07_425723 988 36 fluorescence fluorescence NN 10_1101-2021_01_07_425723 988 37 intensity intensity NN 10_1101-2021_01_07_425723 988 38 of of IN 10_1101-2021_01_07_425723 988 39 PI pi NN 10_1101-2021_01_07_425723 988 40 - - HYPH 10_1101-2021_01_07_425723 988 41 negative negative JJ 10_1101-2021_01_07_425723 988 42 cells cell NNS 10_1101-2021_01_07_425723 988 43 with with IN 10_1101-2021_01_07_425723 988 44 pooled pool VBN 10_1101-2021_01_07_425723 988 45 variance variance NN 10_1101-2021_01_07_425723 988 46 from from IN 10_1101-2021_01_07_425723 988 47 the the DT 10_1101-2021_01_07_425723 988 48 biological biological JJ 10_1101-2021_01_07_425723 988 49 replicate replicate VB 10_1101-2021_01_07_425723 988 50 971 971 CD 10_1101-2021_01_07_425723 988 51 cultures culture NNS 10_1101-2021_01_07_425723 988 52 . . . 10_1101-2021_01_07_425723 989 1 See see VB 10_1101-2021_01_07_425723 989 2 legend legend NN 10_1101-2021_01_07_425723 989 3 Fig Fig NNP 10_1101-2021_01_07_425723 989 4 . . . 10_1101-2021_01_07_425723 990 1 4 4 CD 10_1101-2021_01_07_425723 990 2 for for IN 10_1101-2021_01_07_425723 990 3 experimental experimental JJ 10_1101-2021_01_07_425723 990 4 details detail NNS 10_1101-2021_01_07_425723 990 5 . . . 10_1101-2021_01_07_425723 991 1 972 972 CD 10_1101-2021_01_07_425723 991 2 .CC .CC NFP 10_1101-2021_01_07_425723 991 3 - - HYPH 10_1101-2021_01_07_425723 991 4 BY by IN 10_1101-2021_01_07_425723 991 5 - - HYPH 10_1101-2021_01_07_425723 991 6 NC NC NNP 10_1101-2021_01_07_425723 991 7 - - HYPH 10_1101-2021_01_07_425723 991 8 ND ND NNP 10_1101-2021_01_07_425723 991 9 4.0 4.0 CD 10_1101-2021_01_07_425723 991 10 International International NNP 10_1101-2021_01_07_425723 991 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 991 12 under under IN 10_1101-2021_01_07_425723 991 13 a a DT 10_1101-2021_01_07_425723 991 14 ( ( -LRB- 10_1101-2021_01_07_425723 991 15 which which WDT 10_1101-2021_01_07_425723 991 16 was be VBD 10_1101-2021_01_07_425723 991 17 not not RB 10_1101-2021_01_07_425723 991 18 certified certify VBN 10_1101-2021_01_07_425723 991 19 by by IN 10_1101-2021_01_07_425723 991 20 peer peer NN 10_1101-2021_01_07_425723 991 21 review review NN 10_1101-2021_01_07_425723 991 22 ) ) -RRB- 10_1101-2021_01_07_425723 991 23 is be VBZ 10_1101-2021_01_07_425723 991 24 the the DT 10_1101-2021_01_07_425723 991 25 author author NN 10_1101-2021_01_07_425723 991 26 / / SYM 10_1101-2021_01_07_425723 991 27 funder funder NN 10_1101-2021_01_07_425723 991 28 , , , 10_1101-2021_01_07_425723 991 29 who who WP 10_1101-2021_01_07_425723 991 30 has have VBZ 10_1101-2021_01_07_425723 991 31 granted grant VBN 10_1101-2021_01_07_425723 991 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 991 33 a a DT 10_1101-2021_01_07_425723 991 34 license license NN 10_1101-2021_01_07_425723 991 35 to to TO 10_1101-2021_01_07_425723 991 36 display display VB 10_1101-2021_01_07_425723 991 37 the the DT 10_1101-2021_01_07_425723 991 38 preprint preprint NN 10_1101-2021_01_07_425723 991 39 in in IN 10_1101-2021_01_07_425723 991 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 991 41 . . . 10_1101-2021_01_07_425723 992 1 It -PRON- PRP 10_1101-2021_01_07_425723 992 2 is be VBZ 10_1101-2021_01_07_425723 992 3 made make VBN 10_1101-2021_01_07_425723 992 4 The the DT 10_1101-2021_01_07_425723 992 5 copyright copyright NN 10_1101-2021_01_07_425723 992 6 holder holder NN 10_1101-2021_01_07_425723 992 7 for for IN 10_1101-2021_01_07_425723 992 8 this this DT 10_1101-2021_01_07_425723 992 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 992 10 version version NN 10_1101-2021_01_07_425723 992 11 posted post VBD 10_1101-2021_01_07_425723 992 12 January January NNP 10_1101-2021_01_07_425723 992 13 8 8 CD 10_1101-2021_01_07_425723 992 14 , , , 10_1101-2021_01_07_425723 992 15 2021 2021 CD 10_1101-2021_01_07_425723 992 16 . . . 10_1101-2021_01_07_425723 992 17 ; ; : 10_1101-2021_01_07_425723 992 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 992 19 : : : 10_1101-2021_01_07_425723 992 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 992 21 preprint preprint NN 10_1101-2021_01_07_425723 992 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 992 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ RB 10_1101-2021_01_07_425723 992 24 58 58 CD 10_1101-2021_01_07_425723 992 25 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 992 26 Fig Fig NNP 10_1101-2021_01_07_425723 992 27 . . . 10_1101-2021_01_07_425723 993 1 8 8 CD 10_1101-2021_01_07_425723 993 2 | | CD 10_1101-2021_01_07_425723 993 3 Flow Flow NNP 10_1101-2021_01_07_425723 993 4 cytometry cytometry NN 10_1101-2021_01_07_425723 993 5 gating gate VBG 10_1101-2021_01_07_425723 993 6 strategy strategy NN 10_1101-2021_01_07_425723 993 7 of of IN 10_1101-2021_01_07_425723 993 8 both both DT 10_1101-2021_01_07_425723 993 9 K. K. NNP 10_1101-2021_01_07_425723 993 10 marxianus marxianus NN 10_1101-2021_01_07_425723 993 11 ( ( -LRB- 10_1101-2021_01_07_425723 993 12 left leave VBD 10_1101-2021_01_07_425723 993 13 panel panel NN 10_1101-2021_01_07_425723 993 14 ) ) -RRB- 10_1101-2021_01_07_425723 993 15 and and CC 10_1101-2021_01_07_425723 993 16 S. S. NNP 10_1101-2021_01_07_425723 993 17 973 973 CD 10_1101-2021_01_07_425723 993 18 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 993 19 ( ( -LRB- 10_1101-2021_01_07_425723 993 20 right right JJ 10_1101-2021_01_07_425723 993 21 panel panel NN 10_1101-2021_01_07_425723 993 22 ) ) -RRB- 10_1101-2021_01_07_425723 993 23 samples sample NNS 10_1101-2021_01_07_425723 993 24 . . . 10_1101-2021_01_07_425723 994 1 Gates gate NNS 10_1101-2021_01_07_425723 994 2 were be VBD 10_1101-2021_01_07_425723 994 3 set set VBN 10_1101-2021_01_07_425723 994 4 per per IN 10_1101-2021_01_07_425723 994 5 one one CD 10_1101-2021_01_07_425723 994 6 species specie NNS 10_1101-2021_01_07_425723 994 7 for for IN 10_1101-2021_01_07_425723 994 8 all all DT 10_1101-2021_01_07_425723 994 9 samples sample NNS 10_1101-2021_01_07_425723 994 10 independent independent JJ 10_1101-2021_01_07_425723 994 11 of of IN 10_1101-2021_01_07_425723 994 12 NBDC NBDC NNP 10_1101-2021_01_07_425723 994 13 974 974 CD 10_1101-2021_01_07_425723 994 14 staining staining NN 10_1101-2021_01_07_425723 994 15 . . . 10_1101-2021_01_07_425723 995 1 Density density NN 10_1101-2021_01_07_425723 995 2 of of IN 10_1101-2021_01_07_425723 995 3 events event NNS 10_1101-2021_01_07_425723 995 4 were be VBD 10_1101-2021_01_07_425723 995 5 calculated calculate VBN 10_1101-2021_01_07_425723 995 6 by by IN 10_1101-2021_01_07_425723 995 7 FlowJo flowjo DT 10_1101-2021_01_07_425723 995 8 software software NN 10_1101-2021_01_07_425723 995 9 and and CC 10_1101-2021_01_07_425723 995 10 represented represent VBD 10_1101-2021_01_07_425723 995 11 in in IN 10_1101-2021_01_07_425723 995 12 pseudo pseudo NN 10_1101-2021_01_07_425723 995 13 - - NN 10_1101-2021_01_07_425723 995 14 color color NN 10_1101-2021_01_07_425723 995 15 ( ( -LRB- 10_1101-2021_01_07_425723 995 16 blue blue JJ 10_1101-2021_01_07_425723 995 17 975 975 CD 10_1101-2021_01_07_425723 995 18 low low JJ 10_1101-2021_01_07_425723 995 19 density density NN 10_1101-2021_01_07_425723 995 20 , , , 10_1101-2021_01_07_425723 995 21 red red JJ 10_1101-2021_01_07_425723 995 22 high high JJ 10_1101-2021_01_07_425723 995 23 - - HYPH 10_1101-2021_01_07_425723 995 24 density density NN 10_1101-2021_01_07_425723 995 25 ) ) -RRB- 10_1101-2021_01_07_425723 995 26 . . . 10_1101-2021_01_07_425723 996 1 The the DT 10_1101-2021_01_07_425723 996 2 gate gate NN 10_1101-2021_01_07_425723 996 3 between between IN 10_1101-2021_01_07_425723 996 4 PI pi NN 10_1101-2021_01_07_425723 996 5 - - HYPH 10_1101-2021_01_07_425723 996 6 negative negative JJ 10_1101-2021_01_07_425723 996 7 and and CC 10_1101-2021_01_07_425723 996 8 PI pi NN 10_1101-2021_01_07_425723 996 9 - - HYPH 10_1101-2021_01_07_425723 996 10 positive positive JJ 10_1101-2021_01_07_425723 996 11 was be VBD 10_1101-2021_01_07_425723 996 12 inside inside IN 10_1101-2021_01_07_425723 996 13 the the DT 10_1101-2021_01_07_425723 996 14 “ " `` 10_1101-2021_01_07_425723 996 15 Cells Cells NNPS 10_1101-2021_01_07_425723 996 16 ” " '' 10_1101-2021_01_07_425723 996 17 976 976 CD 10_1101-2021_01_07_425723 996 18 gated gate VBN 10_1101-2021_01_07_425723 996 19 - - HYPH 10_1101-2021_01_07_425723 996 20 population population NN 10_1101-2021_01_07_425723 996 21 . . . 10_1101-2021_01_07_425723 997 1 977 977 CD 10_1101-2021_01_07_425723 997 2 .CC .CC : 10_1101-2021_01_07_425723 997 3 - - HYPH 10_1101-2021_01_07_425723 997 4 BY by IN 10_1101-2021_01_07_425723 997 5 - - HYPH 10_1101-2021_01_07_425723 997 6 NC NC NNP 10_1101-2021_01_07_425723 997 7 - - HYPH 10_1101-2021_01_07_425723 997 8 ND ND NNP 10_1101-2021_01_07_425723 997 9 4.0 4.0 CD 10_1101-2021_01_07_425723 997 10 International International NNP 10_1101-2021_01_07_425723 997 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 997 12 under under IN 10_1101-2021_01_07_425723 997 13 a a DT 10_1101-2021_01_07_425723 997 14 ( ( -LRB- 10_1101-2021_01_07_425723 997 15 which which WDT 10_1101-2021_01_07_425723 997 16 was be VBD 10_1101-2021_01_07_425723 997 17 not not RB 10_1101-2021_01_07_425723 997 18 certified certify VBN 10_1101-2021_01_07_425723 997 19 by by IN 10_1101-2021_01_07_425723 997 20 peer peer NN 10_1101-2021_01_07_425723 997 21 review review NN 10_1101-2021_01_07_425723 997 22 ) ) -RRB- 10_1101-2021_01_07_425723 997 23 is be VBZ 10_1101-2021_01_07_425723 997 24 the the DT 10_1101-2021_01_07_425723 997 25 author author NN 10_1101-2021_01_07_425723 997 26 / / SYM 10_1101-2021_01_07_425723 997 27 funder funder NN 10_1101-2021_01_07_425723 997 28 , , , 10_1101-2021_01_07_425723 997 29 who who WP 10_1101-2021_01_07_425723 997 30 has have VBZ 10_1101-2021_01_07_425723 997 31 granted grant VBN 10_1101-2021_01_07_425723 997 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 997 33 a a DT 10_1101-2021_01_07_425723 997 34 license license NN 10_1101-2021_01_07_425723 997 35 to to TO 10_1101-2021_01_07_425723 997 36 display display VB 10_1101-2021_01_07_425723 997 37 the the DT 10_1101-2021_01_07_425723 997 38 preprint preprint NN 10_1101-2021_01_07_425723 997 39 in in IN 10_1101-2021_01_07_425723 997 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 997 41 . . . 10_1101-2021_01_07_425723 998 1 It -PRON- PRP 10_1101-2021_01_07_425723 998 2 is be VBZ 10_1101-2021_01_07_425723 998 3 made make VBN 10_1101-2021_01_07_425723 998 4 The the DT 10_1101-2021_01_07_425723 998 5 copyright copyright NN 10_1101-2021_01_07_425723 998 6 holder holder NN 10_1101-2021_01_07_425723 998 7 for for IN 10_1101-2021_01_07_425723 998 8 this this DT 10_1101-2021_01_07_425723 998 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 998 10 version version NN 10_1101-2021_01_07_425723 998 11 posted post VBD 10_1101-2021_01_07_425723 998 12 January January NNP 10_1101-2021_01_07_425723 998 13 8 8 CD 10_1101-2021_01_07_425723 998 14 , , , 10_1101-2021_01_07_425723 998 15 2021 2021 CD 10_1101-2021_01_07_425723 998 16 . . . 10_1101-2021_01_07_425723 998 17 ; ; : 10_1101-2021_01_07_425723 998 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 998 19 : : : 10_1101-2021_01_07_425723 998 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 998 21 preprint preprint NN 10_1101-2021_01_07_425723 998 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 998 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 998 24 59 59 CD 10_1101-2021_01_07_425723 998 25 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 998 26 Fig Fig NNP 10_1101-2021_01_07_425723 998 27 . . . 10_1101-2021_01_07_425723 999 1 9 9 CD 10_1101-2021_01_07_425723 999 2 | | NNP 10_1101-2021_01_07_425723 999 3 Cross cross NN 10_1101-2021_01_07_425723 999 4 - - NN 10_1101-2021_01_07_425723 999 5 validation validation NN 10_1101-2021_01_07_425723 999 6 of of IN 10_1101-2021_01_07_425723 999 7 oxygen oxygen NN 10_1101-2021_01_07_425723 999 8 - - HYPH 10_1101-2021_01_07_425723 999 9 limited limit VBN 10_1101-2021_01_07_425723 999 10 and and CC 10_1101-2021_01_07_425723 999 11 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 999 12 growth growth NN 10_1101-2021_01_07_425723 999 13 of of IN 10_1101-2021_01_07_425723 999 14 K. K. NNP 10_1101-2021_01_07_425723 999 15 marxianus marxianus NNP 10_1101-2021_01_07_425723 999 16 978 978 CD 10_1101-2021_01_07_425723 999 17 IMX2323 IMX2323 NNP 10_1101-2021_01_07_425723 999 18 . . . 10_1101-2021_01_07_425723 1000 1 Strains strain NNS 10_1101-2021_01_07_425723 1000 2 were be VBD 10_1101-2021_01_07_425723 1000 3 grown grow VBN 10_1101-2021_01_07_425723 1000 4 in in IN 10_1101-2021_01_07_425723 1000 5 shake shake NN 10_1101-2021_01_07_425723 1000 6 - - HYPH 10_1101-2021_01_07_425723 1000 7 flask flask NN 10_1101-2021_01_07_425723 1000 8 cultures culture NNS 10_1101-2021_01_07_425723 1000 9 in in IN 10_1101-2021_01_07_425723 1000 10 an an DT 10_1101-2021_01_07_425723 1000 11 oxygen oxygen NN 10_1101-2021_01_07_425723 1000 12 - - HYPH 10_1101-2021_01_07_425723 1000 13 limited limit VBN 10_1101-2021_01_07_425723 1000 14 ( ( -LRB- 10_1101-2021_01_07_425723 1000 15 a a LS 10_1101-2021_01_07_425723 1000 16 ) ) -RRB- 10_1101-2021_01_07_425723 1000 17 and and CC 10_1101-2021_01_07_425723 1000 18 strict strict JJ 10_1101-2021_01_07_425723 1000 19 anaerobic anaerobic NN 10_1101-2021_01_07_425723 1000 20 979 979 CD 10_1101-2021_01_07_425723 1000 21 environment environment NN 10_1101-2021_01_07_425723 1000 22 ( ( -LRB- 10_1101-2021_01_07_425723 1000 23 b b NN 10_1101-2021_01_07_425723 1000 24 ) ) -RRB- 10_1101-2021_01_07_425723 1000 25 . . . 10_1101-2021_01_07_425723 1001 1 To to TO 10_1101-2021_01_07_425723 1001 2 perform perform VB 10_1101-2021_01_07_425723 1001 3 cross cross NN 10_1101-2021_01_07_425723 1001 4 - - NN 10_1101-2021_01_07_425723 1001 5 validation validation NN 10_1101-2021_01_07_425723 1001 6 between between IN 10_1101-2021_01_07_425723 1001 7 the the DT 10_1101-2021_01_07_425723 1001 8 two two CD 10_1101-2021_01_07_425723 1001 9 parallel parallel JJ 10_1101-2021_01_07_425723 1001 10 running running NN 10_1101-2021_01_07_425723 1001 11 experiments experiment NNS 10_1101-2021_01_07_425723 1001 12 , , , 10_1101-2021_01_07_425723 1001 13 1.5 1.5 CD 10_1101-2021_01_07_425723 1001 14 mL ml NN 10_1101-2021_01_07_425723 1001 15 980 980 CD 10_1101-2021_01_07_425723 1001 16 aliquot aliquot NNS 10_1101-2021_01_07_425723 1001 17 of of IN 10_1101-2021_01_07_425723 1001 18 each each DT 10_1101-2021_01_07_425723 1001 19 culture culture NN 10_1101-2021_01_07_425723 1001 20 was be VBD 10_1101-2021_01_07_425723 1001 21 sealed seal VBN 10_1101-2021_01_07_425723 1001 22 and and CC 10_1101-2021_01_07_425723 1001 23 transferred transfer VBN 10_1101-2021_01_07_425723 1001 24 quickly quickly RB 10_1101-2021_01_07_425723 1001 25 between between IN 10_1101-2021_01_07_425723 1001 26 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 1001 27 chambers chamber NNS 10_1101-2021_01_07_425723 1001 28 and and CC 10_1101-2021_01_07_425723 1001 29 used use VBD 10_1101-2021_01_07_425723 1001 30 to to IN 10_1101-2021_01_07_425723 1001 31 981 981 CD 10_1101-2021_01_07_425723 1001 32 inoculate inoculate VB 10_1101-2021_01_07_425723 1001 33 two two CD 10_1101-2021_01_07_425723 1001 34 shake shake NN 10_1101-2021_01_07_425723 1001 35 - - HYPH 10_1101-2021_01_07_425723 1001 36 flask flask NN 10_1101-2021_01_07_425723 1001 37 cultures culture NNS 10_1101-2021_01_07_425723 1001 38 , , , 10_1101-2021_01_07_425723 1001 39 represented represent VBN 10_1101-2021_01_07_425723 1001 40 with with IN 10_1101-2021_01_07_425723 1001 41 crossed cross VBN 10_1101-2021_01_07_425723 1001 42 - - HYPH 10_1101-2021_01_07_425723 1001 43 arrows arrow NNS 10_1101-2021_01_07_425723 1001 44 ( ( -LRB- 10_1101-2021_01_07_425723 1001 45 ⤮ ⤮ NNP 10_1101-2021_01_07_425723 1001 46 ) ) -RRB- 10_1101-2021_01_07_425723 1001 47 . . . 10_1101-2021_01_07_425723 1002 1 The the DT 10_1101-2021_01_07_425723 1002 2 cultures culture NNS 10_1101-2021_01_07_425723 1002 3 from from IN 10_1101-2021_01_07_425723 1002 4 the the DT 10_1101-2021_01_07_425723 1002 5 strain strain NN 10_1101-2021_01_07_425723 1002 6 982 982 CD 10_1101-2021_01_07_425723 1002 7 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 1002 8 ( ( -LRB- 10_1101-2021_01_07_425723 1002 9 ⤮ ⤮ NNP 10_1101-2021_01_07_425723 1002 10 ) ) -RRB- 10_1101-2021_01_07_425723 1002 11 in in IN 10_1101-2021_01_07_425723 1002 12 the the DT 10_1101-2021_01_07_425723 1002 13 third third JJ 10_1101-2021_01_07_425723 1002 14 transfer transfer NN 10_1101-2021_01_07_425723 1002 15 ( ( -LRB- 10_1101-2021_01_07_425723 1002 16 C3 C3 NNP 10_1101-2021_01_07_425723 1002 17 ) ) -RRB- 10_1101-2021_01_07_425723 1002 18 in in IN 10_1101-2021_01_07_425723 1002 19 the the DT 10_1101-2021_01_07_425723 1002 20 strict strict JJ 10_1101-2021_01_07_425723 1002 21 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 1002 22 environment environment NN 10_1101-2021_01_07_425723 1002 23 ( ( -LRB- 10_1101-2021_01_07_425723 1002 24 b b NN 10_1101-2021_01_07_425723 1002 25 ) ) -RRB- 10_1101-2021_01_07_425723 1002 26 were be VBD 10_1101-2021_01_07_425723 1002 27 hence hence RB 10_1101-2021_01_07_425723 1002 28 inoculated inoculate VBN 10_1101-2021_01_07_425723 1002 29 983 983 CD 10_1101-2021_01_07_425723 1002 30 from from IN 10_1101-2021_01_07_425723 1002 31 an an DT 10_1101-2021_01_07_425723 1002 32 aliquot aliquot NNS 10_1101-2021_01_07_425723 1002 33 of of IN 10_1101-2021_01_07_425723 1002 34 the the DT 10_1101-2021_01_07_425723 1002 35 cultures culture NNS 10_1101-2021_01_07_425723 1002 36 of of IN 10_1101-2021_01_07_425723 1002 37 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 1002 38 ( ( -LRB- 10_1101-2021_01_07_425723 1002 39 C2 c2 NN 10_1101-2021_01_07_425723 1002 40 ) ) -RRB- 10_1101-2021_01_07_425723 1002 41 grown grow VBN 10_1101-2021_01_07_425723 1002 42 in in IN 10_1101-2021_01_07_425723 1002 43 oxygen oxygen NN 10_1101-2021_01_07_425723 1002 44 - - HYPH 10_1101-2021_01_07_425723 1002 45 limited limit VBN 10_1101-2021_01_07_425723 1002 46 environment environment NN 10_1101-2021_01_07_425723 1002 47 ( ( -LRB- 10_1101-2021_01_07_425723 1002 48 a a DT 10_1101-2021_01_07_425723 1002 49 ) ) -RRB- 10_1101-2021_01_07_425723 1002 50 . . . 10_1101-2021_01_07_425723 1003 1 This this DT 10_1101-2021_01_07_425723 1003 2 resulted result VBD 10_1101-2021_01_07_425723 1003 3 984 984 CD 10_1101-2021_01_07_425723 1003 4 in in IN 10_1101-2021_01_07_425723 1003 5 a a DT 10_1101-2021_01_07_425723 1003 6 serial serial JJ 10_1101-2021_01_07_425723 1003 7 transfer transfer NN 10_1101-2021_01_07_425723 1003 8 of of IN 10_1101-2021_01_07_425723 1003 9 26.7 26.7 CD 10_1101-2021_01_07_425723 1003 10 times time NNS 10_1101-2021_01_07_425723 1003 11 dilution dilution NN 10_1101-2021_01_07_425723 1003 12 from from IN 10_1101-2021_01_07_425723 1003 13 transfer transfer NNP 10_1101-2021_01_07_425723 1003 14 C2 c2 NN 10_1101-2021_01_07_425723 1003 15 to to TO 10_1101-2021_01_07_425723 1003 16 C3 c3 VB 10_1101-2021_01_07_425723 1003 17 . . . 10_1101-2021_01_07_425723 1004 1 Aerobic Aerobic NNP 10_1101-2021_01_07_425723 1004 2 grown grow VBD 10_1101-2021_01_07_425723 1004 3 pre pre JJ 10_1101-2021_01_07_425723 1004 4 - - NNS 10_1101-2021_01_07_425723 1004 5 cultures culture NNS 10_1101-2021_01_07_425723 1004 6 were be VBD 10_1101-2021_01_07_425723 1004 7 used use VBN 10_1101-2021_01_07_425723 1004 8 985 985 CD 10_1101-2021_01_07_425723 1004 9 to to TO 10_1101-2021_01_07_425723 1004 10 inoculate inoculate VB 10_1101-2021_01_07_425723 1004 11 the the DT 10_1101-2021_01_07_425723 1004 12 first first JJ 10_1101-2021_01_07_425723 1004 13 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 1004 14 culture culture NN 10_1101-2021_01_07_425723 1004 15 on on IN 10_1101-2021_01_07_425723 1004 16 SMG SMG NNP 10_1101-2021_01_07_425723 1004 17 - - HYPH 10_1101-2021_01_07_425723 1004 18 urea urea NNP 10_1101-2021_01_07_425723 1004 19 containing contain VBG 10_1101-2021_01_07_425723 1004 20 50 50 CD 10_1101-2021_01_07_425723 1004 21 g·L-1 g·l-1 CD 10_1101-2021_01_07_425723 1004 22 glucose glucose NN 10_1101-2021_01_07_425723 1004 23 and and CC 10_1101-2021_01_07_425723 1004 24 Tween Tween NNP 10_1101-2021_01_07_425723 1004 25 80 80 CD 10_1101-2021_01_07_425723 1004 26 . . . 10_1101-2021_01_07_425723 1005 1 Data datum NNS 10_1101-2021_01_07_425723 1005 2 986 986 CD 10_1101-2021_01_07_425723 1005 3 depicted depict VBN 10_1101-2021_01_07_425723 1005 4 are be VBP 10_1101-2021_01_07_425723 1005 5 of of IN 10_1101-2021_01_07_425723 1005 6 each each DT 10_1101-2021_01_07_425723 1005 7 replicate replicate NN 10_1101-2021_01_07_425723 1005 8 culture culture NN 10_1101-2021_01_07_425723 1005 9 ( ( -LRB- 10_1101-2021_01_07_425723 1005 10 points point NNS 10_1101-2021_01_07_425723 1005 11 ) ) -RRB- 10_1101-2021_01_07_425723 1005 12 and and CC 10_1101-2021_01_07_425723 1005 13 the the DT 10_1101-2021_01_07_425723 1005 14 mean mean JJ 10_1101-2021_01_07_425723 1005 15 ( ( -LRB- 10_1101-2021_01_07_425723 1005 16 dotted dotted JJ 10_1101-2021_01_07_425723 1005 17 line line NN 10_1101-2021_01_07_425723 1005 18 ) ) -RRB- 10_1101-2021_01_07_425723 1005 19 from from IN 10_1101-2021_01_07_425723 1005 20 independent independent JJ 10_1101-2021_01_07_425723 1005 21 biological biological JJ 10_1101-2021_01_07_425723 1005 22 987 987 CD 10_1101-2021_01_07_425723 1005 23 duplicate duplicate JJ 10_1101-2021_01_07_425723 1005 24 cultures culture NNS 10_1101-2021_01_07_425723 1005 25 , , , 10_1101-2021_01_07_425723 1005 26 serial serial JJ 10_1101-2021_01_07_425723 1005 27 transfers transfer NNS 10_1101-2021_01_07_425723 1005 28 cultures culture NNS 10_1101-2021_01_07_425723 1005 29 are be VBP 10_1101-2021_01_07_425723 1005 30 represented represent VBN 10_1101-2021_01_07_425723 1005 31 with with IN 10_1101-2021_01_07_425723 1005 32 the the DT 10_1101-2021_01_07_425723 1005 33 number number NN 10_1101-2021_01_07_425723 1005 34 of of IN 10_1101-2021_01_07_425723 1005 35 respective respective JJ 10_1101-2021_01_07_425723 1005 36 transfer transfer NN 10_1101-2021_01_07_425723 1005 37 ( ( -LRB- 10_1101-2021_01_07_425723 1005 38 C1 C1 NNP 10_1101-2021_01_07_425723 1005 39 - - HYPH 10_1101-2021_01_07_425723 1005 40 988 988 CD 10_1101-2021_01_07_425723 1005 41 3 3 CD 10_1101-2021_01_07_425723 1005 42 ) ) -RRB- 10_1101-2021_01_07_425723 1005 43 .989 .989 CD 10_1101-2021_01_07_425723 1005 44 .CC .CC NFP 10_1101-2021_01_07_425723 1005 45 - - HYPH 10_1101-2021_01_07_425723 1005 46 BY by IN 10_1101-2021_01_07_425723 1005 47 - - HYPH 10_1101-2021_01_07_425723 1005 48 NC NC NNP 10_1101-2021_01_07_425723 1005 49 - - HYPH 10_1101-2021_01_07_425723 1005 50 ND ND NNP 10_1101-2021_01_07_425723 1005 51 4.0 4.0 CD 10_1101-2021_01_07_425723 1005 52 International International NNP 10_1101-2021_01_07_425723 1005 53 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 1005 54 under under IN 10_1101-2021_01_07_425723 1005 55 a a DT 10_1101-2021_01_07_425723 1005 56 ( ( -LRB- 10_1101-2021_01_07_425723 1005 57 which which WDT 10_1101-2021_01_07_425723 1005 58 was be VBD 10_1101-2021_01_07_425723 1005 59 not not RB 10_1101-2021_01_07_425723 1005 60 certified certify VBN 10_1101-2021_01_07_425723 1005 61 by by IN 10_1101-2021_01_07_425723 1005 62 peer peer NN 10_1101-2021_01_07_425723 1005 63 review review NN 10_1101-2021_01_07_425723 1005 64 ) ) -RRB- 10_1101-2021_01_07_425723 1005 65 is be VBZ 10_1101-2021_01_07_425723 1005 66 the the DT 10_1101-2021_01_07_425723 1005 67 author author NN 10_1101-2021_01_07_425723 1005 68 / / SYM 10_1101-2021_01_07_425723 1005 69 funder funder NN 10_1101-2021_01_07_425723 1005 70 , , , 10_1101-2021_01_07_425723 1005 71 who who WP 10_1101-2021_01_07_425723 1005 72 has have VBZ 10_1101-2021_01_07_425723 1005 73 granted grant VBN 10_1101-2021_01_07_425723 1005 74 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 1005 75 a a DT 10_1101-2021_01_07_425723 1005 76 license license NN 10_1101-2021_01_07_425723 1005 77 to to TO 10_1101-2021_01_07_425723 1005 78 display display VB 10_1101-2021_01_07_425723 1005 79 the the DT 10_1101-2021_01_07_425723 1005 80 preprint preprint NN 10_1101-2021_01_07_425723 1005 81 in in IN 10_1101-2021_01_07_425723 1005 82 perpetuity perpetuity NN 10_1101-2021_01_07_425723 1005 83 . . . 10_1101-2021_01_07_425723 1006 1 It -PRON- PRP 10_1101-2021_01_07_425723 1006 2 is be VBZ 10_1101-2021_01_07_425723 1006 3 made make VBN 10_1101-2021_01_07_425723 1006 4 The the DT 10_1101-2021_01_07_425723 1006 5 copyright copyright NN 10_1101-2021_01_07_425723 1006 6 holder holder NN 10_1101-2021_01_07_425723 1006 7 for for IN 10_1101-2021_01_07_425723 1006 8 this this DT 10_1101-2021_01_07_425723 1006 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 1006 10 version version NN 10_1101-2021_01_07_425723 1006 11 posted post VBD 10_1101-2021_01_07_425723 1006 12 January January NNP 10_1101-2021_01_07_425723 1006 13 8 8 CD 10_1101-2021_01_07_425723 1006 14 , , , 10_1101-2021_01_07_425723 1006 15 2021 2021 CD 10_1101-2021_01_07_425723 1006 16 . . . 10_1101-2021_01_07_425723 1006 17 ; ; : 10_1101-2021_01_07_425723 1006 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 1006 19 : : : 10_1101-2021_01_07_425723 1006 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 1006 21 preprint preprint NN 10_1101-2021_01_07_425723 1006 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 1006 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425723 1006 24 60 60 CD 10_1101-2021_01_07_425723 1006 25 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 1006 26 Fig Fig NNP 10_1101-2021_01_07_425723 1006 27 . . . 10_1101-2021_01_07_425723 1007 1 10 10 CD 10_1101-2021_01_07_425723 1007 2 | | NNP 10_1101-2021_01_07_425723 1007 3 Sterol Sterol NNP 10_1101-2021_01_07_425723 1007 4 - - HYPH 10_1101-2021_01_07_425723 1007 5 independent independent JJ 10_1101-2021_01_07_425723 1007 6 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 1007 7 growth growth NN 10_1101-2021_01_07_425723 1007 8 of of IN 10_1101-2021_01_07_425723 1007 9 S. S. NNP 10_1101-2021_01_07_425723 1007 10 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 1007 11 IMX585 IMX585 NNP 10_1101-2021_01_07_425723 1007 12 ( ( -LRB- 10_1101-2021_01_07_425723 1007 13 reference reference NN 10_1101-2021_01_07_425723 1007 14 ) ) -RRB- 10_1101-2021_01_07_425723 1007 15 , , , 10_1101-2021_01_07_425723 1007 16 990 990 CD 10_1101-2021_01_07_425723 1007 17 IMX1438 IMX1438 NNP 10_1101-2021_01_07_425723 1007 18 ( ( -LRB- 10_1101-2021_01_07_425723 1007 19 TtSTC1 ttstc1 NN 10_1101-2021_01_07_425723 1007 20 ) ) -RRB- 10_1101-2021_01_07_425723 1007 21 , , , 10_1101-2021_01_07_425723 1007 22 K. K. NNP 10_1101-2021_01_07_425723 1007 23 marxianus marxianus NN 10_1101-2021_01_07_425723 1007 24 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 1007 25 ( ( -LRB- 10_1101-2021_01_07_425723 1007 26 reference reference NN 10_1101-2021_01_07_425723 1007 27 ) ) -RRB- 10_1101-2021_01_07_425723 1007 28 and and CC 10_1101-2021_01_07_425723 1007 29 IMX2323 IMX2323 NNP 10_1101-2021_01_07_425723 1007 30 ( ( -LRB- 10_1101-2021_01_07_425723 1007 31 TtSTC1 TtSTC1 NNP 10_1101-2021_01_07_425723 1007 32 ) ) -RRB- 10_1101-2021_01_07_425723 1007 33 . . . 10_1101-2021_01_07_425723 1008 1 Aerobic Aerobic NNP 10_1101-2021_01_07_425723 1008 2 grown grow VBN 10_1101-2021_01_07_425723 1008 3 pre-991 pre-991 NN 10_1101-2021_01_07_425723 1008 4 cultures culture NNS 10_1101-2021_01_07_425723 1008 5 were be VBD 10_1101-2021_01_07_425723 1008 6 used use VBN 10_1101-2021_01_07_425723 1008 7 to to TO 10_1101-2021_01_07_425723 1008 8 inoculate inoculate VB 10_1101-2021_01_07_425723 1008 9 shake shake NN 10_1101-2021_01_07_425723 1008 10 - - HYPH 10_1101-2021_01_07_425723 1008 11 flask flask NN 10_1101-2021_01_07_425723 1008 12 cultures culture NNS 10_1101-2021_01_07_425723 1008 13 with with IN 10_1101-2021_01_07_425723 1008 14 SMG SMG NNP 10_1101-2021_01_07_425723 1008 15 - - HYPH 10_1101-2021_01_07_425723 1008 16 urea urea NNP 10_1101-2021_01_07_425723 1008 17 containing contain VBG 10_1101-2021_01_07_425723 1008 18 50 50 CD 10_1101-2021_01_07_425723 1008 19 g·L-1 g·L-1 NNP 10_1101-2021_01_07_425723 1008 20 glucose glucose NN 10_1101-2021_01_07_425723 1008 21 and and CC 10_1101-2021_01_07_425723 1008 22 992 992 CD 10_1101-2021_01_07_425723 1008 23 Tween Tween NNP 10_1101-2021_01_07_425723 1008 24 80 80 CD 10_1101-2021_01_07_425723 1008 25 in in IN 10_1101-2021_01_07_425723 1008 26 a a DT 10_1101-2021_01_07_425723 1008 27 strict strict JJ 10_1101-2021_01_07_425723 1008 28 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 1008 29 environment environment NN 10_1101-2021_01_07_425723 1008 30 at at IN 10_1101-2021_01_07_425723 1008 31 an an DT 10_1101-2021_01_07_425723 1008 32 OD600 od600 NN 10_1101-2021_01_07_425723 1008 33 of of IN 10_1101-2021_01_07_425723 1008 34 0.1 0.1 CD 10_1101-2021_01_07_425723 1008 35 for for IN 10_1101-2021_01_07_425723 1008 36 all all DT 10_1101-2021_01_07_425723 1008 37 strains strain NNS 10_1101-2021_01_07_425723 1008 38 , , , 10_1101-2021_01_07_425723 1008 39 and and CC 10_1101-2021_01_07_425723 1008 40 both both CC 10_1101-2021_01_07_425723 1008 41 at at IN 10_1101-2021_01_07_425723 1008 42 OD600 od600 NN 10_1101-2021_01_07_425723 1008 43 of of IN 10_1101-2021_01_07_425723 1008 44 0.1 0.1 CD 10_1101-2021_01_07_425723 1008 45 and and CC 10_1101-2021_01_07_425723 1008 46 993 993 CD 10_1101-2021_01_07_425723 1008 47 0.6 0.6 CD 10_1101-2021_01_07_425723 1008 48 for for IN 10_1101-2021_01_07_425723 1008 49 NBRC1777 NBRC1777 NNP 10_1101-2021_01_07_425723 1008 50 and and CC 10_1101-2021_01_07_425723 1008 51 IMX2323 IMX2323 NNP 10_1101-2021_01_07_425723 1008 52 . . . 10_1101-2021_01_07_425723 1009 1 Data datum NNS 10_1101-2021_01_07_425723 1009 2 depicted depict VBN 10_1101-2021_01_07_425723 1009 3 are be VBP 10_1101-2021_01_07_425723 1009 4 of of IN 10_1101-2021_01_07_425723 1009 5 each each DT 10_1101-2021_01_07_425723 1009 6 replicate replicate NN 10_1101-2021_01_07_425723 1009 7 culture culture NN 10_1101-2021_01_07_425723 1009 8 ( ( -LRB- 10_1101-2021_01_07_425723 1009 9 points point NNS 10_1101-2021_01_07_425723 1009 10 ) ) -RRB- 10_1101-2021_01_07_425723 1009 11 and and CC 10_1101-2021_01_07_425723 1009 12 the the DT 10_1101-2021_01_07_425723 1009 13 mean mean NNP 10_1101-2021_01_07_425723 1009 14 994 994 CD 10_1101-2021_01_07_425723 1009 15 ( ( -LRB- 10_1101-2021_01_07_425723 1009 16 dotted dotted JJ 10_1101-2021_01_07_425723 1009 17 line line NN 10_1101-2021_01_07_425723 1009 18 ) ) -RRB- 10_1101-2021_01_07_425723 1009 19 from from IN 10_1101-2021_01_07_425723 1009 20 independent independent JJ 10_1101-2021_01_07_425723 1009 21 biological biological JJ 10_1101-2021_01_07_425723 1009 22 duplicate duplicate JJ 10_1101-2021_01_07_425723 1009 23 cultures culture NNS 10_1101-2021_01_07_425723 1009 24 , , , 10_1101-2021_01_07_425723 1009 25 serial serial JJ 10_1101-2021_01_07_425723 1009 26 transfers transfer NNS 10_1101-2021_01_07_425723 1009 27 cultures culture NNS 10_1101-2021_01_07_425723 1009 28 are be VBP 10_1101-2021_01_07_425723 1009 29 represented represent VBN 10_1101-2021_01_07_425723 1009 30 995 995 CD 10_1101-2021_01_07_425723 1009 31 with with IN 10_1101-2021_01_07_425723 1009 32 the the DT 10_1101-2021_01_07_425723 1009 33 number number NN 10_1101-2021_01_07_425723 1009 34 of of IN 10_1101-2021_01_07_425723 1009 35 respective respective JJ 10_1101-2021_01_07_425723 1009 36 transfer transfer NN 10_1101-2021_01_07_425723 1009 37 ( ( -LRB- 10_1101-2021_01_07_425723 1009 38 C1 C1 NNP 10_1101-2021_01_07_425723 1009 39 - - HYPH 10_1101-2021_01_07_425723 1009 40 2 2 CD 10_1101-2021_01_07_425723 1009 41 ) ) -RRB- 10_1101-2021_01_07_425723 1009 42 . . . 10_1101-2021_01_07_425723 1010 1 996 996 CD 10_1101-2021_01_07_425723 1010 2 .CC .CC : 10_1101-2021_01_07_425723 1010 3 - - HYPH 10_1101-2021_01_07_425723 1010 4 BY by IN 10_1101-2021_01_07_425723 1010 5 - - HYPH 10_1101-2021_01_07_425723 1010 6 NC NC NNP 10_1101-2021_01_07_425723 1010 7 - - HYPH 10_1101-2021_01_07_425723 1010 8 ND ND NNP 10_1101-2021_01_07_425723 1010 9 4.0 4.0 CD 10_1101-2021_01_07_425723 1010 10 International International NNP 10_1101-2021_01_07_425723 1010 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 1010 12 under under IN 10_1101-2021_01_07_425723 1010 13 a a DT 10_1101-2021_01_07_425723 1010 14 ( ( -LRB- 10_1101-2021_01_07_425723 1010 15 which which WDT 10_1101-2021_01_07_425723 1010 16 was be VBD 10_1101-2021_01_07_425723 1010 17 not not RB 10_1101-2021_01_07_425723 1010 18 certified certify VBN 10_1101-2021_01_07_425723 1010 19 by by IN 10_1101-2021_01_07_425723 1010 20 peer peer NN 10_1101-2021_01_07_425723 1010 21 review review NN 10_1101-2021_01_07_425723 1010 22 ) ) -RRB- 10_1101-2021_01_07_425723 1010 23 is be VBZ 10_1101-2021_01_07_425723 1010 24 the the DT 10_1101-2021_01_07_425723 1010 25 author author NN 10_1101-2021_01_07_425723 1010 26 / / SYM 10_1101-2021_01_07_425723 1010 27 funder funder NN 10_1101-2021_01_07_425723 1010 28 , , , 10_1101-2021_01_07_425723 1010 29 who who WP 10_1101-2021_01_07_425723 1010 30 has have VBZ 10_1101-2021_01_07_425723 1010 31 granted grant VBN 10_1101-2021_01_07_425723 1010 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 1010 33 a a DT 10_1101-2021_01_07_425723 1010 34 license license NN 10_1101-2021_01_07_425723 1010 35 to to TO 10_1101-2021_01_07_425723 1010 36 display display VB 10_1101-2021_01_07_425723 1010 37 the the DT 10_1101-2021_01_07_425723 1010 38 preprint preprint NN 10_1101-2021_01_07_425723 1010 39 in in IN 10_1101-2021_01_07_425723 1010 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 1010 41 . . . 10_1101-2021_01_07_425723 1011 1 It -PRON- PRP 10_1101-2021_01_07_425723 1011 2 is be VBZ 10_1101-2021_01_07_425723 1011 3 made make VBN 10_1101-2021_01_07_425723 1011 4 The the DT 10_1101-2021_01_07_425723 1011 5 copyright copyright NN 10_1101-2021_01_07_425723 1011 6 holder holder NN 10_1101-2021_01_07_425723 1011 7 for for IN 10_1101-2021_01_07_425723 1011 8 this this DT 10_1101-2021_01_07_425723 1011 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 1011 10 version version NN 10_1101-2021_01_07_425723 1011 11 posted post VBD 10_1101-2021_01_07_425723 1011 12 January January NNP 10_1101-2021_01_07_425723 1011 13 8 8 CD 10_1101-2021_01_07_425723 1011 14 , , , 10_1101-2021_01_07_425723 1011 15 2021 2021 CD 10_1101-2021_01_07_425723 1011 16 . . . 10_1101-2021_01_07_425723 1011 17 ; ; : 10_1101-2021_01_07_425723 1011 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 1011 19 : : : 10_1101-2021_01_07_425723 1011 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 1011 21 preprint preprint NN 10_1101-2021_01_07_425723 1011 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 1011 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 1011 24 61 61 CD 10_1101-2021_01_07_425723 1011 25 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 1011 26 Fig Fig NNP 10_1101-2021_01_07_425723 1011 27 . . . 10_1101-2021_01_07_425723 1012 1 11 11 CD 10_1101-2021_01_07_425723 1012 2 | | JJ 10_1101-2021_01_07_425723 1012 3 CO2 co2 JJ 10_1101-2021_01_07_425723 1012 4 fraction fraction NN 10_1101-2021_01_07_425723 1012 5 in in IN 10_1101-2021_01_07_425723 1012 6 the the DT 10_1101-2021_01_07_425723 1012 7 off off JJ 10_1101-2021_01_07_425723 1012 8 - - HYPH 10_1101-2021_01_07_425723 1012 9 gas gas NN 10_1101-2021_01_07_425723 1012 10 of of IN 10_1101-2021_01_07_425723 1012 11 K. K. NNP 10_1101-2021_01_07_425723 1012 12 marxianus marxianus NN 10_1101-2021_01_07_425723 1012 13 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 1012 14 . . . 10_1101-2021_01_07_425723 1013 1 Production production NN 10_1101-2021_01_07_425723 1013 2 of of IN 10_1101-2021_01_07_425723 1013 3 CO2 CO2 NNP 10_1101-2021_01_07_425723 1013 4 as as IN 10_1101-2021_01_07_425723 1013 5 997 997 CD 10_1101-2021_01_07_425723 1013 6 measured measure VBN 10_1101-2021_01_07_425723 1013 7 by by IN 10_1101-2021_01_07_425723 1013 8 the the DT 10_1101-2021_01_07_425723 1013 9 fraction fraction NN 10_1101-2021_01_07_425723 1013 10 of of IN 10_1101-2021_01_07_425723 1013 11 CO2 CO2 NNP 10_1101-2021_01_07_425723 1013 12 in in IN 10_1101-2021_01_07_425723 1013 13 the the DT 10_1101-2021_01_07_425723 1013 14 off off JJ 10_1101-2021_01_07_425723 1013 15 - - HYPH 10_1101-2021_01_07_425723 1013 16 gas gas NN 10_1101-2021_01_07_425723 1013 17 of of IN 10_1101-2021_01_07_425723 1013 18 the the DT 10_1101-2021_01_07_425723 1013 19 individual individual JJ 10_1101-2021_01_07_425723 1013 20 bioreactor bioreactor NN 10_1101-2021_01_07_425723 1013 21 cultivations cultivation NNS 10_1101-2021_01_07_425723 1013 22 of of IN 10_1101-2021_01_07_425723 1013 23 the the DT 10_1101-2021_01_07_425723 1013 24 K. K. NNP 10_1101-2021_01_07_425723 1013 25 998 998 CD 10_1101-2021_01_07_425723 1013 26 marxianus marxianus NN 10_1101-2021_01_07_425723 1013 27 strain strain NN 10_1101-2021_01_07_425723 1013 28 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 1013 29 on on IN 10_1101-2021_01_07_425723 1013 30 SMG SMG NNP 10_1101-2021_01_07_425723 1013 31 media medium NNS 10_1101-2021_01_07_425723 1013 32 pH ph NN 10_1101-2021_01_07_425723 1013 33 5.0 5.0 CD 10_1101-2021_01_07_425723 1013 34 with with IN 10_1101-2021_01_07_425723 1013 35 20 20 CD 10_1101-2021_01_07_425723 1013 36 g·L-1 g·L-1 NNP 10_1101-2021_01_07_425723 1013 37 glucose glucose NN 10_1101-2021_01_07_425723 1013 38 , , , 10_1101-2021_01_07_425723 1013 39 420 420 CD 10_1101-2021_01_07_425723 1013 40 mg·L-1 mg·l-1 NN 10_1101-2021_01_07_425723 1013 41 Tween Tween NNP 10_1101-2021_01_07_425723 1013 42 80 80 CD 10_1101-2021_01_07_425723 1013 43 over over IN 10_1101-2021_01_07_425723 1013 44 time time NN 10_1101-2021_01_07_425723 1013 45 999 999 CD 10_1101-2021_01_07_425723 1013 46 ( ( -LRB- 10_1101-2021_01_07_425723 1013 47 Left left JJ 10_1101-2021_01_07_425723 1013 48 panels panel NNS 10_1101-2021_01_07_425723 1013 49 ) ) -RRB- 10_1101-2021_01_07_425723 1013 50 . . . 10_1101-2021_01_07_425723 1014 1 The the DT 10_1101-2021_01_07_425723 1014 2 temperature temperature NN 10_1101-2021_01_07_425723 1014 3 profile profile NN 10_1101-2021_01_07_425723 1014 4 was be VBD 10_1101-2021_01_07_425723 1014 5 incrementally incrementally RB 10_1101-2021_01_07_425723 1014 6 increased increase VBN 10_1101-2021_01_07_425723 1014 7 at at IN 10_1101-2021_01_07_425723 1014 8 the the DT 10_1101-2021_01_07_425723 1014 9 beginning beginning NN 10_1101-2021_01_07_425723 1014 10 of of IN 10_1101-2021_01_07_425723 1014 11 a a DT 10_1101-2021_01_07_425723 1014 12 new new JJ 10_1101-2021_01_07_425723 1014 13 batch batch NN 10_1101-2021_01_07_425723 1014 14 cycle cycle NN 10_1101-2021_01_07_425723 1014 15 1000 1000 CD 10_1101-2021_01_07_425723 1014 16 ( ( -LRB- 10_1101-2021_01_07_425723 1014 17 right right JJ 10_1101-2021_01_07_425723 1014 18 panels panel NNS 10_1101-2021_01_07_425723 1014 19 ) ) -RRB- 10_1101-2021_01_07_425723 1014 20 . . . 10_1101-2021_01_07_425723 1015 1 After after IN 10_1101-2021_01_07_425723 1015 2 430 430 CD 10_1101-2021_01_07_425723 1015 3 h h NN 10_1101-2021_01_07_425723 1015 4 the the DT 10_1101-2021_01_07_425723 1015 5 performance performance NN 10_1101-2021_01_07_425723 1015 6 of of IN 10_1101-2021_01_07_425723 1015 7 the the DT 10_1101-2021_01_07_425723 1015 8 off off JJ 10_1101-2021_01_07_425723 1015 9 - - HYPH 10_1101-2021_01_07_425723 1015 10 gas gas NN 10_1101-2021_01_07_425723 1015 11 analyzer analyzer NN 10_1101-2021_01_07_425723 1015 12 of of IN 10_1101-2021_01_07_425723 1015 13 replicate replicate NN 10_1101-2021_01_07_425723 1015 14 M3R M3R NNP 10_1101-2021_01_07_425723 1015 15 deteriorated deteriorate VBD 10_1101-2021_01_07_425723 1015 16 . . . 10_1101-2021_01_07_425723 1016 1 1001 1001 CD 10_1101-2021_01_07_425723 1016 2 .CC .CC , 10_1101-2021_01_07_425723 1016 3 - - HYPH 10_1101-2021_01_07_425723 1016 4 BY by IN 10_1101-2021_01_07_425723 1016 5 - - HYPH 10_1101-2021_01_07_425723 1016 6 NC NC NNP 10_1101-2021_01_07_425723 1016 7 - - HYPH 10_1101-2021_01_07_425723 1016 8 ND ND NNP 10_1101-2021_01_07_425723 1016 9 4.0 4.0 CD 10_1101-2021_01_07_425723 1016 10 International International NNP 10_1101-2021_01_07_425723 1016 11 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 1016 12 under under IN 10_1101-2021_01_07_425723 1016 13 a a DT 10_1101-2021_01_07_425723 1016 14 ( ( -LRB- 10_1101-2021_01_07_425723 1016 15 which which WDT 10_1101-2021_01_07_425723 1016 16 was be VBD 10_1101-2021_01_07_425723 1016 17 not not RB 10_1101-2021_01_07_425723 1016 18 certified certify VBN 10_1101-2021_01_07_425723 1016 19 by by IN 10_1101-2021_01_07_425723 1016 20 peer peer NN 10_1101-2021_01_07_425723 1016 21 review review NN 10_1101-2021_01_07_425723 1016 22 ) ) -RRB- 10_1101-2021_01_07_425723 1016 23 is be VBZ 10_1101-2021_01_07_425723 1016 24 the the DT 10_1101-2021_01_07_425723 1016 25 author author NN 10_1101-2021_01_07_425723 1016 26 / / SYM 10_1101-2021_01_07_425723 1016 27 funder funder NN 10_1101-2021_01_07_425723 1016 28 , , , 10_1101-2021_01_07_425723 1016 29 who who WP 10_1101-2021_01_07_425723 1016 30 has have VBZ 10_1101-2021_01_07_425723 1016 31 granted grant VBN 10_1101-2021_01_07_425723 1016 32 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 1016 33 a a DT 10_1101-2021_01_07_425723 1016 34 license license NN 10_1101-2021_01_07_425723 1016 35 to to TO 10_1101-2021_01_07_425723 1016 36 display display VB 10_1101-2021_01_07_425723 1016 37 the the DT 10_1101-2021_01_07_425723 1016 38 preprint preprint NN 10_1101-2021_01_07_425723 1016 39 in in IN 10_1101-2021_01_07_425723 1016 40 perpetuity perpetuity NN 10_1101-2021_01_07_425723 1016 41 . . . 10_1101-2021_01_07_425723 1017 1 It -PRON- PRP 10_1101-2021_01_07_425723 1017 2 is be VBZ 10_1101-2021_01_07_425723 1017 3 made make VBN 10_1101-2021_01_07_425723 1017 4 The the DT 10_1101-2021_01_07_425723 1017 5 copyright copyright NN 10_1101-2021_01_07_425723 1017 6 holder holder NN 10_1101-2021_01_07_425723 1017 7 for for IN 10_1101-2021_01_07_425723 1017 8 this this DT 10_1101-2021_01_07_425723 1017 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 1017 10 version version NN 10_1101-2021_01_07_425723 1017 11 posted post VBD 10_1101-2021_01_07_425723 1017 12 January January NNP 10_1101-2021_01_07_425723 1017 13 8 8 CD 10_1101-2021_01_07_425723 1017 14 , , , 10_1101-2021_01_07_425723 1017 15 2021 2021 CD 10_1101-2021_01_07_425723 1017 16 . . . 10_1101-2021_01_07_425723 1017 17 ; ; : 10_1101-2021_01_07_425723 1017 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 1017 19 : : : 10_1101-2021_01_07_425723 1017 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 1017 21 preprint preprint NN 10_1101-2021_01_07_425723 1017 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 1017 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ RB 10_1101-2021_01_07_425723 1017 24 62 62 CD 10_1101-2021_01_07_425723 1017 25 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 1017 26 Table Table NNP 10_1101-2021_01_07_425723 1017 27 1 1 CD 10_1101-2021_01_07_425723 1017 28 | | NNP 10_1101-2021_01_07_425723 1017 29 Mutations Mutations NNPS 10_1101-2021_01_07_425723 1017 30 identified identify VBN 10_1101-2021_01_07_425723 1017 31 by by IN 10_1101-2021_01_07_425723 1017 32 whole whole JJ 10_1101-2021_01_07_425723 1017 33 - - HYPH 10_1101-2021_01_07_425723 1017 34 genome genome RB 10_1101-2021_01_07_425723 1017 35 sequencing sequencing NN 10_1101-2021_01_07_425723 1017 36 in in IN 10_1101-2021_01_07_425723 1017 37 comparison comparison NN 10_1101-2021_01_07_425723 1017 38 to to IN 10_1101-2021_01_07_425723 1017 39 the the DT 10_1101-2021_01_07_425723 1017 40 1002 1002 CD 10_1101-2021_01_07_425723 1017 41 reference reference NN 10_1101-2021_01_07_425723 1017 42 K. K. NNP 10_1101-2021_01_07_425723 1017 43 marxianus marxianus NN 10_1101-2021_01_07_425723 1017 44 strain strain VBP 10_1101-2021_01_07_425723 1017 45 IMX2323 IMX2323 NNP 10_1101-2021_01_07_425723 1017 46 . . . 10_1101-2021_01_07_425723 1018 1 Overview overview NN 10_1101-2021_01_07_425723 1018 2 of of IN 10_1101-2021_01_07_425723 1018 3 mutations mutation NNS 10_1101-2021_01_07_425723 1018 4 detected detect VBN 10_1101-2021_01_07_425723 1018 5 in in IN 10_1101-2021_01_07_425723 1018 6 the the DT 10_1101-2021_01_07_425723 1018 7 strains strain NNS 10_1101-2021_01_07_425723 1018 8 after after IN 10_1101-2021_01_07_425723 1018 9 selected select VBN 10_1101-2021_01_07_425723 1018 10 for for IN 10_1101-2021_01_07_425723 1018 11 1003 1003 CD 10_1101-2021_01_07_425723 1018 12 strict strict JJ 10_1101-2021_01_07_425723 1018 13 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 1018 14 growth growth NN 10_1101-2021_01_07_425723 1018 15 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 1018 16 , , , 10_1101-2021_01_07_425723 1018 17 IMS1131 IMS1131 NNP 10_1101-2021_01_07_425723 1018 18 , , , 10_1101-2021_01_07_425723 1018 19 IMS1132 IMS1132 NNP 10_1101-2021_01_07_425723 1018 20 , , , 10_1101-2021_01_07_425723 1018 21 IMS1133 IMS1133 NNP 10_1101-2021_01_07_425723 1018 22 compared compare VBN 10_1101-2021_01_07_425723 1018 23 to to IN 10_1101-2021_01_07_425723 1018 24 the the DT 10_1101-2021_01_07_425723 1018 25 TtSTC1 ttstc1 NN 10_1101-2021_01_07_425723 1018 26 engineered engineer VBN 10_1101-2021_01_07_425723 1018 27 1004 1004 CD 10_1101-2021_01_07_425723 1018 28 strain strain NN 10_1101-2021_01_07_425723 1018 29 ( ( -LRB- 10_1101-2021_01_07_425723 1018 30 IMX2323 IMX2323 NNP 10_1101-2021_01_07_425723 1018 31 ) ) -RRB- 10_1101-2021_01_07_425723 1018 32 . . . 10_1101-2021_01_07_425723 1019 1 Resequencing resequence VBG 10_1101-2021_01_07_425723 1019 2 of of IN 10_1101-2021_01_07_425723 1019 3 IMS1111 IMS1111 NNP 10_1101-2021_01_07_425723 1019 4 after after IN 10_1101-2021_01_07_425723 1019 5 4 4 CD 10_1101-2021_01_07_425723 1019 6 transfers transfer NNS 10_1101-2021_01_07_425723 1019 7 in in IN 10_1101-2021_01_07_425723 1019 8 strict strict JJ 10_1101-2021_01_07_425723 1019 9 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 1019 10 conditions condition NNS 10_1101-2021_01_07_425723 1019 11 is be VBZ 10_1101-2021_01_07_425723 1019 12 for for IN 10_1101-2021_01_07_425723 1019 13 clarity clarity NN 10_1101-2021_01_07_425723 1019 14 1005 1005 CD 10_1101-2021_01_07_425723 1019 15 referred refer VBD 10_1101-2021_01_07_425723 1019 16 with with IN 10_1101-2021_01_07_425723 1019 17 the the DT 10_1101-2021_01_07_425723 1019 18 strain strain NN 10_1101-2021_01_07_425723 1019 19 name name NN 10_1101-2021_01_07_425723 1019 20 IMS1115 IMS1115 NNP 10_1101-2021_01_07_425723 1019 21 . . . 10_1101-2021_01_07_425723 1020 1 Overview overview NN 10_1101-2021_01_07_425723 1020 2 of of IN 10_1101-2021_01_07_425723 1020 3 mutations mutation NNS 10_1101-2021_01_07_425723 1020 4 of of IN 10_1101-2021_01_07_425723 1020 5 the the DT 10_1101-2021_01_07_425723 1020 6 bioreactor bioreactor NN 10_1101-2021_01_07_425723 1020 7 populations population NNS 10_1101-2021_01_07_425723 1020 8 after after IN 10_1101-2021_01_07_425723 1020 9 1006 1006 CD 10_1101-2021_01_07_425723 1020 10 prolonged prolong VBD 10_1101-2021_01_07_425723 1020 11 selection selection NN 10_1101-2021_01_07_425723 1020 12 for for IN 10_1101-2021_01_07_425723 1020 13 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 1020 14 growth growth NN 10_1101-2021_01_07_425723 1020 15 at at IN 10_1101-2021_01_07_425723 1020 16 elevated elevated JJ 10_1101-2021_01_07_425723 1020 17 temperatures temperature NNS 10_1101-2021_01_07_425723 1020 18 , , , 10_1101-2021_01_07_425723 1020 19 represented represent VBN 10_1101-2021_01_07_425723 1020 20 by by IN 10_1101-2021_01_07_425723 1020 21 the the DT 10_1101-2021_01_07_425723 1020 22 bioreactor bioreactor NN 10_1101-2021_01_07_425723 1020 23 1007 1007 CD 10_1101-2021_01_07_425723 1020 24 replicates replicates NNP 10_1101-2021_01_07_425723 1020 25 ( ( -LRB- 10_1101-2021_01_07_425723 1020 26 M3R M3R NNP 10_1101-2021_01_07_425723 1020 27 , , , 10_1101-2021_01_07_425723 1020 28 M5R M5R NNP 10_1101-2021_01_07_425723 1020 29 , , , 10_1101-2021_01_07_425723 1020 30 and and CC 10_1101-2021_01_07_425723 1020 31 M6L m6l NN 10_1101-2021_01_07_425723 1020 32 ) ) -RRB- 10_1101-2021_01_07_425723 1020 33 . . . 10_1101-2021_01_07_425723 1021 1 Mutations mutation NNS 10_1101-2021_01_07_425723 1021 2 in in IN 10_1101-2021_01_07_425723 1021 3 coding code VBG 10_1101-2021_01_07_425723 1021 4 regions region NNS 10_1101-2021_01_07_425723 1021 5 are be VBP 10_1101-2021_01_07_425723 1021 6 annotated annotate VBN 10_1101-2021_01_07_425723 1021 7 as as IN 10_1101-2021_01_07_425723 1021 8 synonymous synonymous JJ 10_1101-2021_01_07_425723 1021 9 ( ( -LRB- 10_1101-2021_01_07_425723 1021 10 SYN SYN NNP 10_1101-2021_01_07_425723 1021 11 ) ) -RRB- 10_1101-2021_01_07_425723 1021 12 , , , 10_1101-2021_01_07_425723 1021 13 non-1008 non-1008 JJ 10_1101-2021_01_07_425723 1021 14 synonymous synonymous JJ 10_1101-2021_01_07_425723 1021 15 ( ( -LRB- 10_1101-2021_01_07_425723 1021 16 NSY NSY NNP 10_1101-2021_01_07_425723 1021 17 ) ) -RRB- 10_1101-2021_01_07_425723 1021 18 , , , 10_1101-2021_01_07_425723 1021 19 insertion insertion NN 10_1101-2021_01_07_425723 1021 20 or or CC 10_1101-2021_01_07_425723 1021 21 deletions deletion NNS 10_1101-2021_01_07_425723 1021 22 . . . 10_1101-2021_01_07_425723 1022 1 Mutations mutation NNS 10_1101-2021_01_07_425723 1022 2 in in IN 10_1101-2021_01_07_425723 1022 3 non non JJ 10_1101-2021_01_07_425723 1022 4 - - JJ 10_1101-2021_01_07_425723 1022 5 coding coding JJ 10_1101-2021_01_07_425723 1022 6 regions region NNS 10_1101-2021_01_07_425723 1022 7 are be VBP 10_1101-2021_01_07_425723 1022 8 reported report VBN 10_1101-2021_01_07_425723 1022 9 with with IN 10_1101-2021_01_07_425723 1022 10 the the DT 10_1101-2021_01_07_425723 1022 11 1009 1009 CD 10_1101-2021_01_07_425723 1022 12 identifier identifier NN 10_1101-2021_01_07_425723 1022 13 of of IN 10_1101-2021_01_07_425723 1022 14 the the DT 10_1101-2021_01_07_425723 1022 15 neighboring neighboring NN 10_1101-2021_01_07_425723 1022 16 gene gene NN 10_1101-2021_01_07_425723 1022 17 , , , 10_1101-2021_01_07_425723 1022 18 directionality directionality NN 10_1101-2021_01_07_425723 1022 19 and and CC 10_1101-2021_01_07_425723 1022 20 strand strand NNP 10_1101-2021_01_07_425723 1022 21 ( ( -LRB- 10_1101-2021_01_07_425723 1022 22 + + SYM 10_1101-2021_01_07_425723 1022 23 /- /- . 10_1101-2021_01_07_425723 1022 24 ) ) -RRB- 10_1101-2021_01_07_425723 1022 25 . . . 10_1101-2021_01_07_425723 1023 1 For for IN 10_1101-2021_01_07_425723 1023 2 K. K. NNP 10_1101-2021_01_07_425723 1023 3 marxianus marxianus JJ 10_1101-2021_01_07_425723 1023 4 genes gene NNS 10_1101-2021_01_07_425723 1023 5 , , , 10_1101-2021_01_07_425723 1023 6 corresponding correspond VBG 10_1101-2021_01_07_425723 1023 7 1010 1010 CD 10_1101-2021_01_07_425723 1023 8 S. S. NNP 10_1101-2021_01_07_425723 1023 9 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 1023 10 orthologs ortholog VBZ 10_1101-2021_01_07_425723 1023 11 with with IN 10_1101-2021_01_07_425723 1023 12 the the DT 10_1101-2021_01_07_425723 1023 13 S288C S288C NNP 10_1101-2021_01_07_425723 1023 14 identifier identifier NNP 10_1101-2021_01_07_425723 1023 15 are be VBP 10_1101-2021_01_07_425723 1023 16 listed list VBN 10_1101-2021_01_07_425723 1023 17 if if IN 10_1101-2021_01_07_425723 1023 18 applicable applicable JJ 10_1101-2021_01_07_425723 1023 19 . . . 10_1101-2021_01_07_425723 1024 1 QD QD NNP 10_1101-2021_01_07_425723 1024 2 refers refer VBZ 10_1101-2021_01_07_425723 1024 3 to to IN 10_1101-2021_01_07_425723 1024 4 quality quality NN 10_1101-2021_01_07_425723 1024 5 by by IN 10_1101-2021_01_07_425723 1024 6 depth depth NN 10_1101-2021_01_07_425723 1024 7 1011 1011 CD 10_1101-2021_01_07_425723 1024 8 calculated calculate VBN 10_1101-2021_01_07_425723 1024 9 by by IN 10_1101-2021_01_07_425723 1024 10 GATK GATK NNP 10_1101-2021_01_07_425723 1024 11 and and CC 10_1101-2021_01_07_425723 1024 12 genotyping genotyping NNP 10_1101-2021_01_07_425723 1024 13 overviews overview NNS 10_1101-2021_01_07_425723 1024 14 are be VBP 10_1101-2021_01_07_425723 1024 15 given give VBN 10_1101-2021_01_07_425723 1024 16 per per IN 10_1101-2021_01_07_425723 1024 17 strain strain NN 10_1101-2021_01_07_425723 1024 18 using use VBG 10_1101-2021_01_07_425723 1024 19 the the DT 10_1101-2021_01_07_425723 1024 20 GATK GATK NNP 10_1101-2021_01_07_425723 1024 21 fields field VBZ 10_1101-2021_01_07_425723 1024 22 GT GT NNP 10_1101-2021_01_07_425723 1024 23 : : : 10_1101-2021_01_07_425723 1024 24 1/1 1/1 CD 10_1101-2021_01_07_425723 1024 25 for for IN 10_1101-2021_01_07_425723 1024 26 1012 1012 CD 10_1101-2021_01_07_425723 1024 27 homozygous homozygous JJ 10_1101-2021_01_07_425723 1024 28 alternative alternative NN 10_1101-2021_01_07_425723 1024 29 , , , 10_1101-2021_01_07_425723 1024 30 1/0 1/0 CD 10_1101-2021_01_07_425723 1024 31 for for IN 10_1101-2021_01_07_425723 1024 32 heterozygous heterozygous JJ 10_1101-2021_01_07_425723 1024 33 , , , 10_1101-2021_01_07_425723 1024 34 AD ad NN 10_1101-2021_01_07_425723 1024 35 : : : 10_1101-2021_01_07_425723 1024 36 allelic allelic JJ 10_1101-2021_01_07_425723 1024 37 depth depth NN 10_1101-2021_01_07_425723 1024 38 ( ( -LRB- 10_1101-2021_01_07_425723 1024 39 number number NN 10_1101-2021_01_07_425723 1024 40 of of IN 10_1101-2021_01_07_425723 1024 41 reads read NNS 10_1101-2021_01_07_425723 1024 42 per per IN 10_1101-2021_01_07_425723 1024 43 reference reference NN 10_1101-2021_01_07_425723 1024 44 and and CC 10_1101-2021_01_07_425723 1024 45 1013 1013 CD 10_1101-2021_01_07_425723 1024 46 alternative alternative JJ 10_1101-2021_01_07_425723 1024 47 alleles allele NNS 10_1101-2021_01_07_425723 1024 48 called call VBD 10_1101-2021_01_07_425723 1024 49 ) ) -RRB- 10_1101-2021_01_07_425723 1024 50 , , , 10_1101-2021_01_07_425723 1024 51 DP dp NN 10_1101-2021_01_07_425723 1024 52 : : : 10_1101-2021_01_07_425723 1024 53 approximate approximate RB 10_1101-2021_01_07_425723 1024 54 read read VBD 10_1101-2021_01_07_425723 1024 55 depth depth NN 10_1101-2021_01_07_425723 1024 56 at at IN 10_1101-2021_01_07_425723 1024 57 the the DT 10_1101-2021_01_07_425723 1024 58 corresponding correspond VBG 10_1101-2021_01_07_425723 1024 59 genomic genomic JJ 10_1101-2021_01_07_425723 1024 60 position position NN 10_1101-2021_01_07_425723 1024 61 , , , 10_1101-2021_01_07_425723 1024 62 and and CC 10_1101-2021_01_07_425723 1024 63 GQ GQ NNP 10_1101-2021_01_07_425723 1024 64 : : : 10_1101-2021_01_07_425723 1024 65 1014 1014 CD 10_1101-2021_01_07_425723 1024 66 genotype genotype NNP 10_1101-2021_01_07_425723 1024 67 quality quality NN 10_1101-2021_01_07_425723 1024 68 . . . 10_1101-2021_01_07_425723 1025 1 NA NA NNP 10_1101-2021_01_07_425723 1025 2 indicates indicate VBZ 10_1101-2021_01_07_425723 1025 3 variants variant NNS 10_1101-2021_01_07_425723 1025 4 were be VBD 10_1101-2021_01_07_425723 1025 5 not not RB 10_1101-2021_01_07_425723 1025 6 called call VBN 10_1101-2021_01_07_425723 1025 7 in in IN 10_1101-2021_01_07_425723 1025 8 that that DT 10_1101-2021_01_07_425723 1025 9 position position NN 10_1101-2021_01_07_425723 1025 10 in in IN 10_1101-2021_01_07_425723 1025 11 the the DT 10_1101-2021_01_07_425723 1025 12 corresponding corresponding JJ 10_1101-2021_01_07_425723 1025 13 strain strain NN 10_1101-2021_01_07_425723 1025 14 . . . 10_1101-2021_01_07_425723 1026 1 1015 1015 CD 10_1101-2021_01_07_425723 1026 2 Chro Chro NNP 10_1101-2021_01_07_425723 1026 3 mos mos NN 10_1101-2021_01_07_425723 1026 4 ome ome UH 10_1101-2021_01_07_425723 1026 5 Po Po NNP 10_1101-2021_01_07_425723 1026 6 siti siti NNP 10_1101-2021_01_07_425723 1026 7 on on IN 10_1101-2021_01_07_425723 1026 8 Descri Descri NNP 10_1101-2021_01_07_425723 1026 9 ption ption NN 10_1101-2021_01_07_425723 1026 10 Type Type NNP 10_1101-2021_01_07_425723 1026 11 Kmar Kmar NNP 10_1101-2021_01_07_425723 1026 12 ID ID NNP 10_1101-2021_01_07_425723 1026 13 S28 S28 NNP 10_1101-2021_01_07_425723 1026 14 8cSy 8csy CD 10_1101-2021_01_07_425723 1026 15 stID stID NNP 10_1101-2021_01_07_425723 1026 16 G G NNP 10_1101-2021_01_07_425723 1026 17 e e NN 10_1101-2021_01_07_425723 1026 18 n n NN 10_1101-2021_01_07_425723 1026 19 e e NN 10_1101-2021_01_07_425723 1026 20 Q q NN 10_1101-2021_01_07_425723 1026 21 D d NN 10_1101-2021_01_07_425723 1026 22 IM IM NNP 10_1101-2021_01_07_425723 1026 23 X2 x2 NN 10_1101-2021_01_07_425723 1026 24 32 32 CD 10_1101-2021_01_07_425723 1026 25 3 3 CD 10_1101-2021_01_07_425723 1026 26 IMS11 IMS11 NNP 10_1101-2021_01_07_425723 1026 27 11 11 CD 10_1101-2021_01_07_425723 1026 28 IMS IMS NNP 10_1101-2021_01_07_425723 1026 29 113 113 CD 10_1101-2021_01_07_425723 1026 30 1 1 CD 10_1101-2021_01_07_425723 1026 31 IMS1 ims1 CD 10_1101-2021_01_07_425723 1026 32 132 132 CD 10_1101-2021_01_07_425723 1026 33 IMS IMS NNP 10_1101-2021_01_07_425723 1026 34 113 113 CD 10_1101-2021_01_07_425723 1026 35 3 3 CD 10_1101-2021_01_07_425723 1026 36 IMS11 IMS11 NNP 10_1101-2021_01_07_425723 1026 37 15 15 CD 10_1101-2021_01_07_425723 1026 38 M3R M3R NNP 10_1101-2021_01_07_425723 1026 39 M5R M5R NNP 10_1101-2021_01_07_425723 1026 40 M6L M6L NNP 10_1101-2021_01_07_425723 1026 41 Mutation Mutation NNP 10_1101-2021_01_07_425723 1026 42 spectra spectra NN 10_1101-2021_01_07_425723 1026 43 of of IN 10_1101-2021_01_07_425723 1026 44 IMX2323 IMX2323 NNP 10_1101-2021_01_07_425723 1026 45 derived derive VBD 10_1101-2021_01_07_425723 1026 46 single single JJ 10_1101-2021_01_07_425723 1026 47 isolates isolate NNS 10_1101-2021_01_07_425723 1026 48 after after IN 10_1101-2021_01_07_425723 1026 49 selection selection NN 10_1101-2021_01_07_425723 1026 50 for for IN 10_1101-2021_01_07_425723 1026 51 strict strict JJ 10_1101-2021_01_07_425723 1026 52 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 1026 53 growth growth NN 10_1101-2021_01_07_425723 1026 54 3 3 CD 10_1101-2021_01_07_425723 1026 55 89 89 CD 10_1101-2021_01_07_425723 1026 56 78 78 CD 10_1101-2021_01_07_425723 1026 57 44 44 CD 10_1101-2021_01_07_425723 1026 58 Asp- Asp- NNP 10_1101-2021_01_07_425723 1026 59 747- 747- CD 10_1101-2021_01_07_425723 1026 60 Asp Asp NNP 10_1101-2021_01_07_425723 1026 61 CDS:(S cds:(s NN 10_1101-2021_01_07_425723 1026 62 YN YN NNP 10_1101-2021_01_07_425723 1026 63 ) ) -RRB- 10_1101-2021_01_07_425723 1026 64 TPUv TPUv VBZ 10_1101-2021_01_07_425723 1026 65 2_00 2_00 CD 10_1101-2021_01_07_425723 1026 66 2092 2092 CD 10_1101-2021_01_07_425723 1026 67 YDR YDR NNP 10_1101-2021_01_07_425723 1026 68 283 283 CD 10_1101-2021_01_07_425723 1026 69 C c NN 10_1101-2021_01_07_425723 1026 70 G g NN 10_1101-2021_01_07_425723 1026 71 cn cn NN 10_1101-2021_01_07_425723 1026 72 2 2 CD 10_1101-2021_01_07_425723 1026 73 3 3 CD 10_1101-2021_01_07_425723 1026 74 2 2 CD 10_1101-2021_01_07_425723 1026 75 NA NA NNP 10_1101-2021_01_07_425723 1026 76 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 77 , , , 10_1101-2021_01_07_425723 1026 78 120:1 120:1 CD 10_1101-2021_01_07_425723 1026 79 20:99 20:99 CD 10_1101-2021_01_07_425723 1026 80 NA na CD 10_1101-2021_01_07_425723 1026 81 NA NA NNP 10_1101-2021_01_07_425723 1026 82 NA NA NNP 10_1101-2021_01_07_425723 1026 83 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 84 , , , 10_1101-2021_01_07_425723 1026 85 105:1 105:1 CD 10_1101-2021_01_07_425723 1026 86 05:99 05:99 CD 10_1101-2021_01_07_425723 1026 87 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 88 , , , 10_1101-2021_01_07_425723 1026 89 99:9 99:9 CD 10_1101-2021_01_07_425723 1026 90 9:99 9:99 CD 10_1101-2021_01_07_425723 1026 91 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 92 , , , 10_1101-2021_01_07_425723 1026 93 110:1 110:1 CD 10_1101-2021_01_07_425723 1026 94 10:99 10:99 CD 10_1101-2021_01_07_425723 1026 95 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 96 , , , 10_1101-2021_01_07_425723 1026 97 118:1 118:1 CD 10_1101-2021_01_07_425723 1026 98 18:99 18:99 CD 10_1101-2021_01_07_425723 1026 99 8 8 CD 10_1101-2021_01_07_425723 1026 100 59 59 CD 10_1101-2021_01_07_425723 1026 101 15 15 CD 10_1101-2021_01_07_425723 1026 102 6 6 CD 10_1101-2021_01_07_425723 1026 103 codon codon NN 10_1101-2021_01_07_425723 1026 104 : : : 10_1101-2021_01_07_425723 1026 105 TCA TCA NNP 10_1101-2021_01_07_425723 1026 106 CDS CDS NNP 10_1101-2021_01_07_425723 1026 107 : : : 10_1101-2021_01_07_425723 1026 108 IN in IN 10_1101-2021_01_07_425723 1026 109 SERTI SERTI NNP 10_1101-2021_01_07_425723 1026 110 ON[1 ON[1 NNP 10_1101-2021_01_07_425723 1026 111 ] ] -RRB- 10_1101-2021_01_07_425723 1026 112 TPUv TPUv VBZ 10_1101-2021_01_07_425723 1026 113 2_00 2_00 CD 10_1101-2021_01_07_425723 1026 114 4766 4766 CD 10_1101-2021_01_07_425723 1026 115 Tran Tran NNP 10_1101-2021_01_07_425723 1026 116 spos spo VBD 10_1101-2021_01_07_425723 1026 117 on on IN 10_1101-2021_01_07_425723 1026 118 2 2 CD 10_1101-2021_01_07_425723 1026 119 7 7 CD 10_1101-2021_01_07_425723 1026 120 NA NA NNP 10_1101-2021_01_07_425723 1026 121 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 122 , , , 10_1101-2021_01_07_425723 1026 123 7:7:21 7:7:21 CD 10_1101-2021_01_07_425723 1026 124 NA NA NNP 10_1101-2021_01_07_425723 1026 125 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 126 , , , 10_1101-2021_01_07_425723 1026 127 15:1 15:1 CD 10_1101-2021_01_07_425723 1026 128 5:45 5:45 CD 10_1101-2021_01_07_425723 1026 129 1/1 1/1 CD 10_1101-2021_01_07_425723 1026 130 : : : 10_1101-2021_01_07_425723 1026 131 0,9 0,9 CD 10_1101-2021_01_07_425723 1026 132 : : : 10_1101-2021_01_07_425723 1026 133 9:27 9:27 CD 10_1101-2021_01_07_425723 1026 134 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 135 , , , 10_1101-2021_01_07_425723 1026 136 9:9:27 9:9:27 NNP 10_1101-2021_01_07_425723 1026 137 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 138 , , , 10_1101-2021_01_07_425723 1026 139 12:1 12:1 CD 10_1101-2021_01_07_425723 1026 140 2:36 2:36 CD 10_1101-2021_01_07_425723 1026 141 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 142 , , , 10_1101-2021_01_07_425723 1026 143 7:7:21 7:7:21 NNP 10_1101-2021_01_07_425723 1026 144 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 145 , , , 10_1101-2021_01_07_425723 1026 146 7:7:21 7:7:21 CD 10_1101-2021_01_07_425723 1026 147 8 8 CD 10_1101-2021_01_07_425723 1026 148 55 55 CD 10_1101-2021_01_07_425723 1026 149 04 04 CD 10_1101-2021_01_07_425723 1026 150 50 50 CD 10_1101-2021_01_07_425723 1026 151 Trp- trp- CD 10_1101-2021_01_07_425723 1026 152 350- 350- CD 10_1101-2021_01_07_425723 1026 153 STP STP NNP 10_1101-2021_01_07_425723 1026 154 CDS:(N cds:(n NN 10_1101-2021_01_07_425723 1026 155 ON on NN 10_1101-2021_01_07_425723 1026 156 ) ) -RRB- 10_1101-2021_01_07_425723 1026 157 TPUv TPUv NNS 10_1101-2021_01_07_425723 1026 158 2_00 2_00 CD 10_1101-2021_01_07_425723 1026 159 4999 4999 CD 10_1101-2021_01_07_425723 1026 160 YAL YAL NNP 10_1101-2021_01_07_425723 1026 161 040 040 CD 10_1101-2021_01_07_425723 1026 162 C c NN 10_1101-2021_01_07_425723 1026 163 Cl Cl NNP 10_1101-2021_01_07_425723 1026 164 n n NN 10_1101-2021_01_07_425723 1026 165 3 3 CD 10_1101-2021_01_07_425723 1026 166 2 2 CD 10_1101-2021_01_07_425723 1026 167 3 3 CD 10_1101-2021_01_07_425723 1026 168 NA NA NNP 10_1101-2021_01_07_425723 1026 169 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 170 , , , 10_1101-2021_01_07_425723 1026 171 119:1 119:1 CD 10_1101-2021_01_07_425723 1026 172 19:99 19:99 CD 10_1101-2021_01_07_425723 1026 173 NA na NN 10_1101-2021_01_07_425723 1026 174 NA NA NNP 10_1101-2021_01_07_425723 1026 175 NA NA NNP 10_1101-2021_01_07_425723 1026 176 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 177 , , , 10_1101-2021_01_07_425723 1026 178 143:1 143:1 CD 10_1101-2021_01_07_425723 1026 179 43:99 43:99 CD 10_1101-2021_01_07_425723 1026 180 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 181 , , , 10_1101-2021_01_07_425723 1026 182 89:8 89:8 CD 10_1101-2021_01_07_425723 1026 183 9:99 9:99 CD 10_1101-2021_01_07_425723 1026 184 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 185 , , , 10_1101-2021_01_07_425723 1026 186 117:1 117:1 CD 10_1101-2021_01_07_425723 1026 187 17:99 17:99 CD 10_1101-2021_01_07_425723 1026 188 1/1:1 1/1:1 CD 10_1101-2021_01_07_425723 1026 189 , , , 10_1101-2021_01_07_425723 1026 190 98:99 98:99 CD 10_1101-2021_01_07_425723 1026 191 : : : 10_1101-2021_01_07_425723 1026 192 99 99 CD 10_1101-2021_01_07_425723 1026 193 4 4 CD 10_1101-2021_01_07_425723 1026 194 45 45 CD 10_1101-2021_01_07_425723 1026 195 97 97 CD 10_1101-2021_01_07_425723 1026 196 50 50 CD 10_1101-2021_01_07_425723 1026 197 TPUv2 tpuv2 NN 10_1101-2021_01_07_425723 1026 198 _ _ NNP 10_1101-2021_01_07_425723 1026 199 0026 0026 CD 10_1101-2021_01_07_425723 1026 200 39-T1 39-t1 CD 10_1101-2021_01_07_425723 1026 201 p3UTR p3UTR NNP 10_1101-2021_01_07_425723 1026 202 : : : 10_1101-2021_01_07_425723 1026 203 + + SYM 10_1101-2021_01_07_425723 1026 204 TPUv TPUv NNS 10_1101-2021_01_07_425723 1026 205 2_00 2_00 CD 10_1101-2021_01_07_425723 1026 206 2639 2639 CD 10_1101-2021_01_07_425723 1026 207 YGR YGR NNP 10_1101-2021_01_07_425723 1026 208 156 156 CD 10_1101-2021_01_07_425723 1026 209 W W NNP 10_1101-2021_01_07_425723 1026 210 Pt Pt NNP 10_1101-2021_01_07_425723 1026 211 i1 i1 NN 10_1101-2021_01_07_425723 1026 212 3 3 CD 10_1101-2021_01_07_425723 1026 213 5 5 CD 10_1101-2021_01_07_425723 1026 214 NA NA NNP 10_1101-2021_01_07_425723 1026 215 NA NA NNP 10_1101-2021_01_07_425723 1026 216 1/1 1/1 CD 10_1101-2021_01_07_425723 1026 217 : : : 10_1101-2021_01_07_425723 1026 218 0,9 0,9 CD 10_1101-2021_01_07_425723 1026 219 : : : 10_1101-2021_01_07_425723 1026 220 9:29 9:29 CD 10_1101-2021_01_07_425723 1026 221 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 222 , , , 10_1101-2021_01_07_425723 1026 223 9:11 9:11 CD 10_1101-2021_01_07_425723 1026 224 : : : 10_1101-2021_01_07_425723 1026 225 54 54 CD 10_1101-2021_01_07_425723 1026 226 1/1 1/1 CD 10_1101-2021_01_07_425723 1026 227 : : : 10_1101-2021_01_07_425723 1026 228 0,9 0,9 CD 10_1101-2021_01_07_425723 1026 229 : : : 10_1101-2021_01_07_425723 1026 230 9:38 9:38 CD 10_1101-2021_01_07_425723 1026 231 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 232 , , , 10_1101-2021_01_07_425723 1026 233 4:6:24 4:6:24 NNP 10_1101-2021_01_07_425723 1026 234 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 235 , , , 10_1101-2021_01_07_425723 1026 236 10:1 10:1 CD 10_1101-2021_01_07_425723 1026 237 0:35 0:35 CD 10_1101-2021_01_07_425723 1026 238 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 239 , , , 10_1101-2021_01_07_425723 1026 240 7:7:26 7:7:26 CD 10_1101-2021_01_07_425723 1026 241 NA na TO 10_1101-2021_01_07_425723 1026 242 5 5 CD 10_1101-2021_01_07_425723 1026 243 17 17 CD 10_1101-2021_01_07_425723 1026 244 74 74 CD 10_1101-2021_01_07_425723 1026 245 29 29 CD 10_1101-2021_01_07_425723 1026 246 TPUv2 tpuv2 NN 10_1101-2021_01_07_425723 1026 247 _ _ NNP 10_1101-2021_01_07_425723 1026 248 0031 0031 CD 10_1101-2021_01_07_425723 1026 249 61-T1 61-t1 CD 10_1101-2021_01_07_425723 1026 250 p5UTR p5UTR NNP 10_1101-2021_01_07_425723 1026 251 : : : 10_1101-2021_01_07_425723 1026 252 - - : 10_1101-2021_01_07_425723 1026 253 TPUv TPUv NNS 10_1101-2021_01_07_425723 1026 254 2_00 2_00 CD 10_1101-2021_01_07_425723 1026 255 3161 3161 CD 10_1101-2021_01_07_425723 1026 256 YBR YBR NNP 10_1101-2021_01_07_425723 1026 257 283 283 CD 10_1101-2021_01_07_425723 1026 258 C C NNP 10_1101-2021_01_07_425723 1026 259 Ss Ss NNP 10_1101-2021_01_07_425723 1026 260 h h NN 10_1101-2021_01_07_425723 1026 261 1 1 CD 10_1101-2021_01_07_425723 1026 262 2 2 CD 10_1101-2021_01_07_425723 1026 263 7 7 CD 10_1101-2021_01_07_425723 1026 264 NA NA NNP 10_1101-2021_01_07_425723 1026 265 NA NA NNP 10_1101-2021_01_07_425723 1026 266 1/1 1/1 CD 10_1101-2021_01_07_425723 1026 267 : : : 10_1101-2021_01_07_425723 1026 268 0,9 0,9 CD 10_1101-2021_01_07_425723 1026 269 : : : 10_1101-2021_01_07_425723 1026 270 9:27 9:27 CD 10_1101-2021_01_07_425723 1026 271 NA NA NNP 10_1101-2021_01_07_425723 1026 272 NA NA NNP 10_1101-2021_01_07_425723 1026 273 0/1:1 0/1:1 CD 10_1101-2021_01_07_425723 1026 274 , , , 10_1101-2021_01_07_425723 1026 275 7:8:21 7:8:21 VBG 10_1101-2021_01_07_425723 1026 276 NA na IN 10_1101-2021_01_07_425723 1026 277 NA na IN 10_1101-2021_01_07_425723 1026 278 NA na TO 10_1101-2021_01_07_425723 1026 279 5 5 CD 10_1101-2021_01_07_425723 1026 280 90 90 CD 10_1101-2021_01_07_425723 1026 281 94 94 CD 10_1101-2021_01_07_425723 1026 282 77 77 CD 10_1101-2021_01_07_425723 1026 283 UTP22 UTP22 NNP 10_1101-2021_01_07_425723 1026 284 p5UTR p5UTR NNP 10_1101-2021_01_07_425723 1026 285 : : : 10_1101-2021_01_07_425723 1026 286 + + SYM 10_1101-2021_01_07_425723 1026 287 TPUv TPUv NNS 10_1101-2021_01_07_425723 1026 288 2_00 2_00 CD 10_1101-2021_01_07_425723 1026 289 3518 3518 CD 10_1101-2021_01_07_425723 1026 290 YGR YGR NNP 10_1101-2021_01_07_425723 1026 291 090 090 CD 10_1101-2021_01_07_425723 1026 292 W w NN 10_1101-2021_01_07_425723 1026 293 U u NN 10_1101-2021_01_07_425723 1026 294 tp tp NN 10_1101-2021_01_07_425723 1026 295 2 2 CD 10_1101-2021_01_07_425723 1026 296 2 2 CD 10_1101-2021_01_07_425723 1026 297 3 3 CD 10_1101-2021_01_07_425723 1026 298 5 5 CD 10_1101-2021_01_07_425723 1026 299 NA NA NNP 10_1101-2021_01_07_425723 1026 300 1/1:1 1/1:1 CD 10_1101-2021_01_07_425723 1026 301 , , , 10_1101-2021_01_07_425723 1026 302 11:12 11:12 CD 10_1101-2021_01_07_425723 1026 303 : : : 10_1101-2021_01_07_425723 1026 304 34 34 CD 10_1101-2021_01_07_425723 1026 305 NA na NN 10_1101-2021_01_07_425723 1026 306 NA NA NNP 10_1101-2021_01_07_425723 1026 307 1/1 1/1 CD 10_1101-2021_01_07_425723 1026 308 : : : 10_1101-2021_01_07_425723 1026 309 1,8 1,8 CD 10_1101-2021_01_07_425723 1026 310 : : : 10_1101-2021_01_07_425723 1026 311 9:24 9:24 CD 10_1101-2021_01_07_425723 1026 312 1/1:0 1/1:0 CD 10_1101-2021_01_07_425723 1026 313 , , , 10_1101-2021_01_07_425723 1026 314 11:11 11:11 CD 10_1101-2021_01_07_425723 1026 315 : : SYM 10_1101-2021_01_07_425723 1026 316 36 36 CD 10_1101-2021_01_07_425723 1026 317 NA na NN 10_1101-2021_01_07_425723 1026 318 NA NA NNP 10_1101-2021_01_07_425723 1026 319 NA NA NNP 10_1101-2021_01_07_425723 1026 320 Mutations Mutations NNPS 10_1101-2021_01_07_425723 1026 321 in in IN 10_1101-2021_01_07_425723 1026 322 whole whole JJ 10_1101-2021_01_07_425723 1026 323 populations population NNS 10_1101-2021_01_07_425723 1026 324 after after IN 10_1101-2021_01_07_425723 1026 325 selection selection NN 10_1101-2021_01_07_425723 1026 326 for for IN 10_1101-2021_01_07_425723 1026 327 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 1026 328 growth growth NN 10_1101-2021_01_07_425723 1026 329 at at IN 10_1101-2021_01_07_425723 1026 330 elevated elevated JJ 10_1101-2021_01_07_425723 1026 331 temperatures temperature NNS 10_1101-2021_01_07_425723 1026 332 .CC .CC NFP 10_1101-2021_01_07_425723 1026 333 - - : 10_1101-2021_01_07_425723 1026 334 BY by IN 10_1101-2021_01_07_425723 1026 335 - - HYPH 10_1101-2021_01_07_425723 1026 336 NC NC NNP 10_1101-2021_01_07_425723 1026 337 - - HYPH 10_1101-2021_01_07_425723 1026 338 ND ND NNP 10_1101-2021_01_07_425723 1026 339 4.0 4.0 CD 10_1101-2021_01_07_425723 1026 340 International International NNP 10_1101-2021_01_07_425723 1026 341 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 1026 342 under under IN 10_1101-2021_01_07_425723 1026 343 a a DT 10_1101-2021_01_07_425723 1026 344 ( ( -LRB- 10_1101-2021_01_07_425723 1026 345 which which WDT 10_1101-2021_01_07_425723 1026 346 was be VBD 10_1101-2021_01_07_425723 1026 347 not not RB 10_1101-2021_01_07_425723 1026 348 certified certify VBN 10_1101-2021_01_07_425723 1026 349 by by IN 10_1101-2021_01_07_425723 1026 350 peer peer NN 10_1101-2021_01_07_425723 1026 351 review review NN 10_1101-2021_01_07_425723 1026 352 ) ) -RRB- 10_1101-2021_01_07_425723 1026 353 is be VBZ 10_1101-2021_01_07_425723 1026 354 the the DT 10_1101-2021_01_07_425723 1026 355 author author NN 10_1101-2021_01_07_425723 1026 356 / / SYM 10_1101-2021_01_07_425723 1026 357 funder funder NN 10_1101-2021_01_07_425723 1026 358 , , , 10_1101-2021_01_07_425723 1026 359 who who WP 10_1101-2021_01_07_425723 1026 360 has have VBZ 10_1101-2021_01_07_425723 1026 361 granted grant VBN 10_1101-2021_01_07_425723 1026 362 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 1026 363 a a DT 10_1101-2021_01_07_425723 1026 364 license license NN 10_1101-2021_01_07_425723 1026 365 to to TO 10_1101-2021_01_07_425723 1026 366 display display VB 10_1101-2021_01_07_425723 1026 367 the the DT 10_1101-2021_01_07_425723 1026 368 preprint preprint NN 10_1101-2021_01_07_425723 1026 369 in in IN 10_1101-2021_01_07_425723 1026 370 perpetuity perpetuity NN 10_1101-2021_01_07_425723 1026 371 . . . 10_1101-2021_01_07_425723 1027 1 It -PRON- PRP 10_1101-2021_01_07_425723 1027 2 is be VBZ 10_1101-2021_01_07_425723 1027 3 made make VBN 10_1101-2021_01_07_425723 1027 4 The the DT 10_1101-2021_01_07_425723 1027 5 copyright copyright NN 10_1101-2021_01_07_425723 1027 6 holder holder NN 10_1101-2021_01_07_425723 1027 7 for for IN 10_1101-2021_01_07_425723 1027 8 this this DT 10_1101-2021_01_07_425723 1027 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 1027 10 version version NN 10_1101-2021_01_07_425723 1027 11 posted post VBD 10_1101-2021_01_07_425723 1027 12 January January NNP 10_1101-2021_01_07_425723 1027 13 8 8 CD 10_1101-2021_01_07_425723 1027 14 , , , 10_1101-2021_01_07_425723 1027 15 2021 2021 CD 10_1101-2021_01_07_425723 1027 16 . . . 10_1101-2021_01_07_425723 1027 17 ; ; : 10_1101-2021_01_07_425723 1027 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 1027 19 : : : 10_1101-2021_01_07_425723 1027 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 1027 21 preprint preprint NN 10_1101-2021_01_07_425723 1027 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 1027 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 1027 24 63 63 CD 10_1101-2021_01_07_425723 1027 25 3 3 CD 10_1101-2021_01_07_425723 1027 26 13 13 CD 10_1101-2021_01_07_425723 1027 27 52 52 CD 10_1101-2021_01_07_425723 1027 28 43 43 CD 10_1101-2021_01_07_425723 1027 29 0 0 CD 10_1101-2021_01_07_425723 1027 30 codon codon NN 10_1101-2021_01_07_425723 1027 31 : : : 10_1101-2021_01_07_425723 1027 32 AAT AAT NNP 10_1101-2021_01_07_425723 1027 33 CDS CDS NNP 10_1101-2021_01_07_425723 1027 34 : : : 10_1101-2021_01_07_425723 1027 35 D d NN 10_1101-2021_01_07_425723 1027 36 ELETIO eletio JJ 10_1101-2021_01_07_425723 1027 37 N[-3 N[-3 NNS 10_1101-2021_01_07_425723 1027 38 ] ] -RRB- 10_1101-2021_01_07_425723 1027 39 TPUv TPUv NNS 10_1101-2021_01_07_425723 1027 40 2_00 2_00 CD 10_1101-2021_01_07_425723 1027 41 2327 2327 CD 10_1101-2021_01_07_425723 1027 42 YLR YLR NNP 10_1101-2021_01_07_425723 1027 43 352 352 CD 10_1101-2021_01_07_425723 1027 44 W W NNP 10_1101-2021_01_07_425723 1027 45 Lu Lu NNP 10_1101-2021_01_07_425723 1027 46 g g NN 10_1101-2021_01_07_425723 1027 47 1 1 CD 10_1101-2021_01_07_425723 1027 48 2 2 CD 10_1101-2021_01_07_425723 1027 49 2 2 CD 10_1101-2021_01_07_425723 1027 50 NA na NN 10_1101-2021_01_07_425723 1027 51 NA NA NNP 10_1101-2021_01_07_425723 1027 52 NA NA NNP 10_1101-2021_01_07_425723 1027 53 NA NA NNP 10_1101-2021_01_07_425723 1027 54 NA NA NNP 10_1101-2021_01_07_425723 1027 55 NA NA NNP 10_1101-2021_01_07_425723 1027 56 NA NA NNP 10_1101-2021_01_07_425723 1027 57 NA NA NNP 10_1101-2021_01_07_425723 1027 58 0/1:39 0/1:39 CD 10_1101-2021_01_07_425723 1027 59 , , , 10_1101-2021_01_07_425723 1027 60 65:10 65:10 CD 10_1101-2021_01_07_425723 1027 61 7:99 7:99 CD 10_1101-2021_01_07_425723 1027 62 8 8 CD 10_1101-2021_01_07_425723 1027 63 63 63 CD 10_1101-2021_01_07_425723 1027 64 57 57 CD 10_1101-2021_01_07_425723 1027 65 79 79 CD 10_1101-2021_01_07_425723 1027 66 codon codon NN 10_1101-2021_01_07_425723 1027 67 : : : 10_1101-2021_01_07_425723 1027 68 CAG CAG NNP 10_1101-2021_01_07_425723 1027 69 CDS CDS NNP 10_1101-2021_01_07_425723 1027 70 : : : 10_1101-2021_01_07_425723 1027 71 IN in IN 10_1101-2021_01_07_425723 1027 72 SERTI SERTI NNP 10_1101-2021_01_07_425723 1027 73 ON[9 ON[9 NNP 10_1101-2021_01_07_425723 1027 74 ] ] -RRB- 10_1101-2021_01_07_425723 1027 75 TPUv TPUv NNS 10_1101-2021_01_07_425723 1027 76 2_00 2_00 CD 10_1101-2021_01_07_425723 1027 77 5049 5049 CD 10_1101-2021_01_07_425723 1027 78 No no DT 10_1101-2021_01_07_425723 1027 79 similarity similarity NN 10_1101-2021_01_07_425723 1027 80 2 2 CD 10_1101-2021_01_07_425723 1027 81 6 6 CD 10_1101-2021_01_07_425723 1027 82 NA na NN 10_1101-2021_01_07_425723 1027 83 NA NA NNP 10_1101-2021_01_07_425723 1027 84 NA NA NNP 10_1101-2021_01_07_425723 1027 85 NA NA NNP 10_1101-2021_01_07_425723 1027 86 NA NA NNP 10_1101-2021_01_07_425723 1027 87 NA NA NNP 10_1101-2021_01_07_425723 1027 88 NA NA NNP 10_1101-2021_01_07_425723 1027 89 NA NA NNP 10_1101-2021_01_07_425723 1027 90 0/1:25 0/1:25 CD 10_1101-2021_01_07_425723 1027 91 , , , 10_1101-2021_01_07_425723 1027 92 49:74 49:74 CD 10_1101-2021_01_07_425723 1027 93 : : : 10_1101-2021_01_07_425723 1027 94 99 99 CD 10_1101-2021_01_07_425723 1027 95 1016 1016 CD 10_1101-2021_01_07_425723 1027 96 .CC .CC : 10_1101-2021_01_07_425723 1027 97 - - : 10_1101-2021_01_07_425723 1027 98 BY by IN 10_1101-2021_01_07_425723 1027 99 - - HYPH 10_1101-2021_01_07_425723 1027 100 NC NC NNP 10_1101-2021_01_07_425723 1027 101 - - HYPH 10_1101-2021_01_07_425723 1027 102 ND ND NNP 10_1101-2021_01_07_425723 1027 103 4.0 4.0 CD 10_1101-2021_01_07_425723 1027 104 International International NNP 10_1101-2021_01_07_425723 1027 105 licenseavailable licenseavailable NN 10_1101-2021_01_07_425723 1027 106 under under IN 10_1101-2021_01_07_425723 1027 107 a a DT 10_1101-2021_01_07_425723 1027 108 ( ( -LRB- 10_1101-2021_01_07_425723 1027 109 which which WDT 10_1101-2021_01_07_425723 1027 110 was be VBD 10_1101-2021_01_07_425723 1027 111 not not RB 10_1101-2021_01_07_425723 1027 112 certified certify VBN 10_1101-2021_01_07_425723 1027 113 by by IN 10_1101-2021_01_07_425723 1027 114 peer peer NN 10_1101-2021_01_07_425723 1027 115 review review NN 10_1101-2021_01_07_425723 1027 116 ) ) -RRB- 10_1101-2021_01_07_425723 1027 117 is be VBZ 10_1101-2021_01_07_425723 1027 118 the the DT 10_1101-2021_01_07_425723 1027 119 author author NN 10_1101-2021_01_07_425723 1027 120 / / SYM 10_1101-2021_01_07_425723 1027 121 funder funder NN 10_1101-2021_01_07_425723 1027 122 , , , 10_1101-2021_01_07_425723 1027 123 who who WP 10_1101-2021_01_07_425723 1027 124 has have VBZ 10_1101-2021_01_07_425723 1027 125 granted grant VBN 10_1101-2021_01_07_425723 1027 126 bioRxiv biorxiv IN 10_1101-2021_01_07_425723 1027 127 a a DT 10_1101-2021_01_07_425723 1027 128 license license NN 10_1101-2021_01_07_425723 1027 129 to to TO 10_1101-2021_01_07_425723 1027 130 display display VB 10_1101-2021_01_07_425723 1027 131 the the DT 10_1101-2021_01_07_425723 1027 132 preprint preprint NN 10_1101-2021_01_07_425723 1027 133 in in IN 10_1101-2021_01_07_425723 1027 134 perpetuity perpetuity NN 10_1101-2021_01_07_425723 1027 135 . . . 10_1101-2021_01_07_425723 1028 1 It -PRON- PRP 10_1101-2021_01_07_425723 1028 2 is be VBZ 10_1101-2021_01_07_425723 1028 3 made make VBN 10_1101-2021_01_07_425723 1028 4 The the DT 10_1101-2021_01_07_425723 1028 5 copyright copyright NN 10_1101-2021_01_07_425723 1028 6 holder holder NN 10_1101-2021_01_07_425723 1028 7 for for IN 10_1101-2021_01_07_425723 1028 8 this this DT 10_1101-2021_01_07_425723 1028 9 preprintthis preprintthis NN 10_1101-2021_01_07_425723 1028 10 version version NN 10_1101-2021_01_07_425723 1028 11 posted post VBD 10_1101-2021_01_07_425723 1028 12 January January NNP 10_1101-2021_01_07_425723 1028 13 8 8 CD 10_1101-2021_01_07_425723 1028 14 , , , 10_1101-2021_01_07_425723 1028 15 2021 2021 CD 10_1101-2021_01_07_425723 1028 16 . . . 10_1101-2021_01_07_425723 1028 17 ; ; : 10_1101-2021_01_07_425723 1028 18 https://doi.org/10.1101/2021.01.07.425723doi https://doi.org/10.1101/2021.01.07.425723doi ADD 10_1101-2021_01_07_425723 1028 19 : : : 10_1101-2021_01_07_425723 1028 20 bioRxiv biorxiv VB 10_1101-2021_01_07_425723 1028 21 preprint preprint NN 10_1101-2021_01_07_425723 1028 22 https://doi.org/10.1101/2021.01.07.425723 https://doi.org/10.1101/2021.01.07.425723 UH 10_1101-2021_01_07_425723 1028 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425723 1028 24 Abstract Abstract NNP 10_1101-2021_01_07_425723 1028 25 Results Results NNPS 10_1101-2021_01_07_425723 1028 26 K. K. NNP 10_1101-2021_01_07_425723 1028 27 marxianus marxianus NN 10_1101-2021_01_07_425723 1028 28 and and CC 10_1101-2021_01_07_425723 1028 29 S. S. NNP 10_1101-2021_01_07_425723 1028 30 cerevisiae cerevisiae NNS 10_1101-2021_01_07_425723 1028 31 show show VBP 10_1101-2021_01_07_425723 1028 32 different different JJ 10_1101-2021_01_07_425723 1028 33 physiological physiological JJ 10_1101-2021_01_07_425723 1028 34 responses response NNS 10_1101-2021_01_07_425723 1028 35 to to IN 10_1101-2021_01_07_425723 1028 36 extreme extreme JJ 10_1101-2021_01_07_425723 1028 37 oxygen oxygen NN 10_1101-2021_01_07_425723 1028 38 limitation limitation NN 10_1101-2021_01_07_425723 1028 39 Transcriptional transcriptional JJ 10_1101-2021_01_07_425723 1028 40 responses response NNS 10_1101-2021_01_07_425723 1028 41 of of IN 10_1101-2021_01_07_425723 1028 42 K. K. NNP 10_1101-2021_01_07_425723 1028 43 marxianus marxianus NN 10_1101-2021_01_07_425723 1028 44 to to IN 10_1101-2021_01_07_425723 1028 45 oxygen oxygen NN 10_1101-2021_01_07_425723 1028 46 limitation limitation NN 10_1101-2021_01_07_425723 1028 47 involve involve VBP 10_1101-2021_01_07_425723 1028 48 ergosterol ergosterol JJ 10_1101-2021_01_07_425723 1028 49 metabolism metabolism NN 10_1101-2021_01_07_425723 1028 50 Absence Absence NNP 10_1101-2021_01_07_425723 1028 51 of of IN 10_1101-2021_01_07_425723 1028 52 sterol sterol JJ 10_1101-2021_01_07_425723 1028 53 import import NN 10_1101-2021_01_07_425723 1028 54 in in IN 10_1101-2021_01_07_425723 1028 55 K. K. NNP 10_1101-2021_01_07_425723 1028 56 marxianus marxianus NN 10_1101-2021_01_07_425723 1028 57 Engineering Engineering NNP 10_1101-2021_01_07_425723 1028 58 K. K. NNP 10_1101-2021_01_07_425723 1028 59 marxianus marxianus NN 10_1101-2021_01_07_425723 1028 60 for for IN 10_1101-2021_01_07_425723 1028 61 oxygen oxygen NN 10_1101-2021_01_07_425723 1028 62 - - HYPH 10_1101-2021_01_07_425723 1028 63 independent independent JJ 10_1101-2021_01_07_425723 1028 64 growth growth NN 10_1101-2021_01_07_425723 1028 65 Test test NN 10_1101-2021_01_07_425723 1028 66 of of IN 10_1101-2021_01_07_425723 1028 67 anaerobic anaerobic JJ 10_1101-2021_01_07_425723 1028 68 thermotolerance thermotolerance NN 10_1101-2021_01_07_425723 1028 69 and and CC 10_1101-2021_01_07_425723 1028 70 selection selection NN 10_1101-2021_01_07_425723 1028 71 for for IN 10_1101-2021_01_07_425723 1028 72 fast fast JJ 10_1101-2021_01_07_425723 1028 73 growing grow VBG 10_1101-2021_01_07_425723 1028 74 anaerobes anaerobe NNS 10_1101-2021_01_07_425723 1028 75 Discussion Discussion NNP 10_1101-2021_01_07_425723 1028 76 online online JJ 10_1101-2021_01_07_425723 1028 77 Methods Methods NNP 10_1101-2021_01_07_425723 1028 78 Yeast Yeast NNP 10_1101-2021_01_07_425723 1028 79 strains strain NNS 10_1101-2021_01_07_425723 1028 80 , , , 10_1101-2021_01_07_425723 1028 81 maintenance maintenance NN 10_1101-2021_01_07_425723 1028 82 and and CC 10_1101-2021_01_07_425723 1028 83 shake shake NN 10_1101-2021_01_07_425723 1028 84 - - HYPH 10_1101-2021_01_07_425723 1028 85 flask flask NN 10_1101-2021_01_07_425723 1028 86 cultivation cultivation NN 10_1101-2021_01_07_425723 1028 87 Expression Expression NNP 10_1101-2021_01_07_425723 1028 88 cassette cassette NN 10_1101-2021_01_07_425723 1028 89 and and CC 10_1101-2021_01_07_425723 1028 90 plasmid plasmid NN 10_1101-2021_01_07_425723 1028 91 construction construction NN 10_1101-2021_01_07_425723 1028 92 Strain Strain NNP 10_1101-2021_01_07_425723 1028 93 construction construction NN 10_1101-2021_01_07_425723 1028 94 Chemostat chemostat JJ 10_1101-2021_01_07_425723 1028 95 cultivation cultivation NN 10_1101-2021_01_07_425723 1028 96 Metabolite metabolite JJ 10_1101-2021_01_07_425723 1028 97 analysis analysis NN 10_1101-2021_01_07_425723 1028 98 Gas Gas NNP 10_1101-2021_01_07_425723 1028 99 analysis analysis NN 10_1101-2021_01_07_425723 1028 100 Ethanol ethanol NN 10_1101-2021_01_07_425723 1028 101 evaporation evaporation NN 10_1101-2021_01_07_425723 1028 102 rate rate NN 10_1101-2021_01_07_425723 1028 103 Lipid Lipid NNP 10_1101-2021_01_07_425723 1028 104 extractions extraction NNS 10_1101-2021_01_07_425723 1028 105 & & CC 10_1101-2021_01_07_425723 1028 106 GC GC NNP 10_1101-2021_01_07_425723 1028 107 analysis analysis NN 10_1101-2021_01_07_425723 1028 108 Sterol Sterol NNP 10_1101-2021_01_07_425723 1028 109 uptake uptake VBD 10_1101-2021_01_07_425723 1028 110 assay assay NN 10_1101-2021_01_07_425723 1028 111 Long long RB 10_1101-2021_01_07_425723 1028 112 read read VBD 10_1101-2021_01_07_425723 1028 113 sequencing sequencing NN 10_1101-2021_01_07_425723 1028 114 , , , 10_1101-2021_01_07_425723 1028 115 assembly assembly NN 10_1101-2021_01_07_425723 1028 116 , , , 10_1101-2021_01_07_425723 1028 117 and and CC 10_1101-2021_01_07_425723 1028 118 annotation annotation NN 10_1101-2021_01_07_425723 1028 119 Illumina Illumina NNP 10_1101-2021_01_07_425723 1028 120 sequencing sequence VBG 10_1101-2021_01_07_425723 1028 121 RNA RNA NNP 10_1101-2021_01_07_425723 1028 122 isolation isolation NN 10_1101-2021_01_07_425723 1028 123 , , , 10_1101-2021_01_07_425723 1028 124 sequencing sequencing NN 10_1101-2021_01_07_425723 1028 125 and and CC 10_1101-2021_01_07_425723 1028 126 transcriptome transcriptome DT 10_1101-2021_01_07_425723 1028 127 analysis analysis NN 10_1101-2021_01_07_425723 1028 128 Anaerobic anaerobic JJ 10_1101-2021_01_07_425723 1028 129 growth growth NN 10_1101-2021_01_07_425723 1028 130 experiments experiment VBZ 10_1101-2021_01_07_425723 1028 131 Laboratory Laboratory NNP 10_1101-2021_01_07_425723 1028 132 evolution evolution NN 10_1101-2021_01_07_425723 1028 133 in in IN 10_1101-2021_01_07_425723 1028 134 low low JJ 10_1101-2021_01_07_425723 1028 135 oxygen oxygen NN 10_1101-2021_01_07_425723 1028 136 atmosphere atmosphere NN 10_1101-2021_01_07_425723 1028 137 Laboratory Laboratory NNP 10_1101-2021_01_07_425723 1028 138 evolution evolution NN 10_1101-2021_01_07_425723 1028 139 in in IN 10_1101-2021_01_07_425723 1028 140 sequential sequential JJ 10_1101-2021_01_07_425723 1028 141 batch batch NN 10_1101-2021_01_07_425723 1028 142 reactors reactor NNS 10_1101-2021_01_07_425723 1028 143 Statistics Statistics NNPS 10_1101-2021_01_07_425723 1028 144 Data Data NNP 10_1101-2021_01_07_425723 1028 145 availability availability NN 10_1101-2021_01_07_425723 1028 146 Code Code NNP 10_1101-2021_01_07_425723 1028 147 availability availability NN 10_1101-2021_01_07_425723 1028 148 Author author NN 10_1101-2021_01_07_425723 1028 149 ’s ’s POS 10_1101-2021_01_07_425723 1028 150 contributions contribution NNS 10_1101-2021_01_07_425723 1028 151 Acknowledgements Acknowledgements NNP 10_1101-2021_01_07_425723 1028 152 Competing compete VBG 10_1101-2021_01_07_425723 1028 153 interest interest NN 10_1101-2021_01_07_425723 1028 154 Funding funding NN 10_1101-2021_01_07_425723 1028 155 References References NNPS 10_1101-2021_01_07_425723 1028 156 Description description NN 10_1101-2021_01_07_425723 1028 157 of of IN 10_1101-2021_01_07_425723 1028 158 Additional Additional NNP 10_1101-2021_01_07_425723 1028 159 Supplementary Supplementary NNP 10_1101-2021_01_07_425723 1028 160 Files Files NNPS 10_1101-2021_01_07_425723 1028 161 Reporting Reporting NNP 10_1101-2021_01_07_425723 1028 162 summary summary NN 10_1101-2021_01_07_425723 1028 163 Supplemental supplemental JJ 10_1101-2021_01_07_425723 1028 164 material material NN 10_1101-2021_01_07_425723 1028 165 for for IN 10_1101-2021_01_07_425723 1028 166 : : :