id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004866-slqje8cq	Orlik-Eisel, Gabriele	The cytotoxin of Pseudomonas aeruginosa: Cytotoxicity requires proteolytic activation	1990		.txt	text/plain	3495	258	56	Like [(125)I] labeled material from Pseudomonas aeruginosa this polypeptide bound to membrane preparations from Ehrlich ascites cells, as evidenced by sedimentation through a sucrose gradient at neutral pH. Expression of the cytotoxin gene in Escherichia coli was inducible with IPTG (compare lanes 1 and 2 in Fig. 5 B) , again yielding material that migrated like the non-processed form of the cytotoxin in SDS-PAGE (compare with lane 4). We have established the sequence of a pore-forming cytotoxin from Pseudomonas aeruginosa by determining the amino-terminal amino acid sequence of the purified protein and sequencing of the structural gene, as identified by a pool of synthetic oligonucleotides. Within the N-terminal domain, a remarkable homology to a pentapeptide consensus sequence (TonB-box), commonly found in outer membrane receptor proteins of the Escherichia coli iron transport system, was detected. Pseudomonas aerugmosa cytotoxin: The nucleotide sequence of the gene and the mechanism of activation of the protein	./cache/cord-004866-slqje8cq.txt	./txt/cord-004866-slqje8cq.txt