id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-260109-vgbrwegt	Charley, B.	Characterization of blood mononuclear cells producing IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)	1990-02-28		.txt	text/plain	3199	217	53	Furthermore, addition of anti-MHC (class II) mAb during PBMC incubation with virus-infected cells reduced IFN yields, suggesting that masking of these surface antigens alters PBMC responsiveness to IFN induction. Moreover, experiments in which IFN0c production was inhibited by monoclonal antibodies (mAb) directed at some epitopes of the TGEV glycoprotein E1 suggested that a defined domain of this transmembrane viral protein played a key role in the IFN0t induction process (Charley and Laurie, 1988) . In the present report, we analysed the nature of lymphocyte subpopulation(s) responsive to IFNa induction by TGEV-infected cell monolayers by using cell-depletion experiments with mAb plus complement. In addition, IFN induction-blocking experiments conducted with anti-MHC (SLA, swine leukocyte antigens) class II mAb without complement suggest a functional role for these membrane molecules in the IFN induction process. However, two preparations of anti-SLA class II mAb (TH22A5 and MSA3) could directly block IFN induction when added during co-incubation of PBMC with i'GEV-infected fixed monolayers or TGE alone (5 independent experiments).	./cache/cord-260109-vgbrwegt.txt	./txt/cord-260109-vgbrwegt.txt