id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-269358-dm3mdqhl	White, L. A.	Inactivation and stability of viral diagnostic reagents treated by gamma radiation	1990-10-31		.txt	text/plain	3180	195	42	Influenza antigens inactivated with 1·7 Mrad displayed comparable potency, sensitivity, specificity and stability to those inactivated by standard procedures with beta-propiolactone (BPL). Gamma radiation is safer than chemical inactivation procedures and is a reliable and effective replacement for BPL in preparing diagnostic reagents. Because of the potential carcinogenic hazard to the laboratorian associated with the use of BPL1' and the deleterious effects of BPL on some hemagglutinating (HA) antigens, our laboratory investigated inactivation ofviral reagents by gamma radiation from a cobalt-60 source. i5 The CDC Inventoryil of reference antigens and antisera were used as test controls and for evaluating the sensitivity and specificity of all radiation-inactivated reagents. Preliminary experiments were conducted to determine the amount of gamma radiation required to inactivate the infectivity of 13 influenza virus strains. These viruses were inactivated by gamma radiation of 0.42 to 3.70 Mrad without adverse effect on the potency, sensitivity or specificity of the antigen.	./cache/cord-269358-dm3mdqhl.txt	./txt/cord-269358-dm3mdqhl.txt