id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-325740-ak7bf0gq	Payne, H. R.	Bovine coronavirus antigen in the host cell plasmalemma	1990-10-31		.txt	text/plain	2437	129	51	Abstract Expression of bovine coronavirus (BCV) antigen in the plasmalemma of epithelioid human rectal tumor (HRT-18) and fibroblastic bovine fetal spleen (BFS) cell lines was traced by immunofluorescence and immunoelectron microscopy facilitated by colloidal gold. Analysis of the labeling characteristics established that the goldmarked-sites represented de novo expression of BCV antigen in the plasma membrane of infected cells. Bovine fetal spleen (BFS) cells are highly susceptible to BCV-induced cell fusion if trypsin is added to the medium of infected cultures. Viral antigen did not increase in the plasma membrane after the onset of cell fusion in infected BFS cell cultures. The differences among infected BFS and HRT-18 cells in the surface expression of viral antigen and fusion might result from the kinetics and mechanisms of protein expression and the differences in the composition of the two plasma membranes.	./cache/cord-325740-ak7bf0gq.txt	./txt/cord-325740-ak7bf0gq.txt