id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-298083-4h3tg6hg	Ho, Tin-Yun	Antigenicity and receptor-binding ability of recombinant SARS coronavirus spike protein	2004-01-23		.txt	text/plain	3557	208	56	In order to analyze the antigenicity and receptor-binding ability of SARS-CoV S protein, we expressed the S protein in Escherichia coli using a pET expression vector. By biotinylated ELISA and Western blot using biotin-labeled S protein as the probe, we identified 130-kDa and 140-kDa proteins in Vero cells that might be the cellular receptors responsible for SARS-CoV infection. Taken together, these results suggested that recombinant S protein exhibited the antigenicity and receptor-binding ability, and it could be a good candidate for further developing SARS vaccine and anti-SARS therapy. These data suggested that comparison of primary amino acid sequences does not provide insight into the receptor-binding specificity or antigenic properties of SARS-CoV S protein. To analyze the antigenicity of recombinant S protein, we performed Western blot and ELISA using sera from SARS patients or from spike-immunized rabbits.	./cache/cord-298083-4h3tg6hg.txt	./txt/cord-298083-4h3tg6hg.txt