id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-339514-0aa58pi6	Ho, Yu	Assembly of human severe acute respiratory syndrome coronavirus-like particles	2004-06-11		.txt	text/plain	2662	140	53	Viral particles of human severe acute respiratory syndrome coronavirus (SARS CoV) consist of three virion structural proteins, including spike protein, membrane protein, and envelope protein. In this report, virus-like particles were assembled in insect cells by the co-infection with recombinant baculoviruses, which separately express one of these three virion proteins. Sucrose gradient purification followed by Western blot analysis and immunogold labeling showed that the spike protein could be incorporated into the virus like particle also. Recombinant baculoviruses encoding E, M, and S protein genes were used to infect Sf21 insect cell, and the expression of each protein was checked by Western blot at 4 dpi (data not shown). The packaging signal of coronavirus RNA was previously identified by using defective interfering viral particles [26] , since the SARS-CoV is a highly infectious virus, it would be much more feasible to identify this signal by using VLPs. Moreover, S protein-containing VLPs should be able to specifically target to the host cell of the SARS CoV and serve as a safe and efficient tool to deliver Western analysis confirmed the presence of S and E proteins mainly in fractions 16-18.	./cache/cord-339514-0aa58pi6.txt	./txt/cord-339514-0aa58pi6.txt