id	sid	tid	token	lemma	pos
cord-010188-884d196k	1	1	key	key	JJ
cord-010188-884d196k	1	2	:	:	:
cord-010188-884d196k	1	3	cord-010188	cord-010188	NNP
cord-010188-884d196k	1	4	-	-	HYPH
cord-010188-884d196k	1	5	884d196k	884d196k	NN
cord-010188-884d196k	1	6	authors	author	NNS
cord-010188-884d196k	1	7	:	:	:
cord-010188-884d196k	1	8	Schlesinger	Schlesinger	NNP
cord-010188-884d196k	1	9	,	,	,
cord-010188-884d196k	1	10	Sondra	Sondra	NNP
cord-010188-884d196k	1	11	title	title	NN
cord-010188-884d196k	1	12	:	:	:
cord-010188-884d196k	1	13	Alphaviruses	Alphaviruses	NNP
cord-010188-884d196k	1	14	—	—	:
cord-010188-884d196k	1	15	vectors	vector	NNS
cord-010188-884d196k	1	16	for	for	IN
cord-010188-884d196k	1	17	the	the	DT
cord-010188-884d196k	1	18	expression	expression	NN
cord-010188-884d196k	1	19	of	of	IN
cord-010188-884d196k	1	20	heterologous	heterologous	JJ
cord-010188-884d196k	1	21	genes	gene	NNS
cord-010188-884d196k	1	22	date	date	NN
cord-010188-884d196k	1	23	:	:	:
cord-010188-884d196k	1	24	2004	2004	CD
cord-010188-884d196k	1	25	-	-	SYM
cord-010188-884d196k	1	26	08	08	CD
cord-010188-884d196k	1	27	-	-	SYM
cord-010188-884d196k	1	28	26	26	CD
cord-010188-884d196k	1	29	journal	journal	NN
cord-010188-884d196k	1	30	:	:	:
cord-010188-884d196k	2	1	Trends	trend	NNS
cord-010188-884d196k	3	1	Biotechnol	Biotechnol	NNP
cord-010188-884d196k	3	2	DOI	DOI	NNP
cord-010188-884d196k	3	3	:	:	:
cord-010188-884d196k	4	1	10.1016/0167	10.1016/0167	CD
cord-010188-884d196k	4	2	-	-	HYPH
cord-010188-884d196k	4	3	7799(93)90070-p	7799(93)90070-p	CD
cord-010188-884d196k	4	4	sha	sha	NNP
cord-010188-884d196k	4	5	:	:	:
cord-010188-884d196k	5	1	654133fc5a86c32e4308b9fbfa434189ae2f5d3f	654133fc5a86c32e4308b9fbfa434189ae2f5d3f	CD
cord-010188-884d196k	5	2	doc_id	doc_id	CD
cord-010188-884d196k	5	3	:	:	:
cord-010188-884d196k	5	4	10188	10188	CD
cord-010188-884d196k	5	5	cord_uid	cord_uid	NNS
cord-010188-884d196k	5	6	:	:	:
cord-010188-884d196k	6	1	884d196k	884d196k	IN
cord-010188-884d196k	6	2	DNA	dna	NN
cord-010188-884d196k	6	3	viruses	virus	NNS
cord-010188-884d196k	6	4	and	and	CC
cord-010188-884d196k	6	5	retroviruses	retrovirus	NNS
cord-010188-884d196k	6	6	are	be	VBP
cord-010188-884d196k	6	7	well	well	RB
cord-010188-884d196k	6	8	established	establish	VBN
cord-010188-884d196k	6	9	as	as	IN
cord-010188-884d196k	6	10	vectors	vector	NNS
cord-010188-884d196k	6	11	for	for	IN
cord-010188-884d196k	6	12	the	the	DT
cord-010188-884d196k	6	13	expression	expression	NN
cord-010188-884d196k	6	14	of	of	IN
cord-010188-884d196k	6	15	heterologous	heterologous	JJ
cord-010188-884d196k	6	16	genes	gene	NNS
cord-010188-884d196k	6	17	,	,	,
cord-010188-884d196k	6	18	but	but	CC
cord-010188-884d196k	6	19	there	there	EX
cord-010188-884d196k	6	20	is	be	VBZ
cord-010188-884d196k	6	21	increasing	increase	VBG
cord-010188-884d196k	6	22	interest	interest	NN
cord-010188-884d196k	6	23	in	in	IN
cord-010188-884d196k	6	24	the	the	DT
cord-010188-884d196k	6	25	possibilities	possibility	NNS
cord-010188-884d196k	6	26	of	of	IN
cord-010188-884d196k	6	27	using	use	VBG
cord-010188-884d196k	6	28	RNA	RNA	NNP
cord-010188-884d196k	6	29	viruses	virus	NNS
cord-010188-884d196k	6	30	,	,	,
cord-010188-884d196k	6	31	which	which	WDT
cord-010188-884d196k	6	32	do	do	VBP
cord-010188-884d196k	6	33	not	not	RB
cord-010188-884d196k	6	34	replicate	replicate	VB
cord-010188-884d196k	6	35	through	through	IN
cord-010188-884d196k	6	36	a	a	DT
cord-010188-884d196k	6	37	DNA	dna	NN
cord-010188-884d196k	6	38	intermediate	intermediate	NN
cord-010188-884d196k	6	39	,	,	,
cord-010188-884d196k	6	40	for	for	IN
cord-010188-884d196k	6	41	this	this	DT
cord-010188-884d196k	6	42	purpose	purpose	NN
cord-010188-884d196k	6	43	.	.	.
cord-010188-884d196k	7	1	This	this	DT
cord-010188-884d196k	7	2	article	article	NN
cord-010188-884d196k	7	3	summarizes	summarize	VBZ
cord-010188-884d196k	7	4	some	some	DT
cord-010188-884d196k	7	5	of	of	IN
cord-010188-884d196k	7	6	the	the	DT
cord-010188-884d196k	7	7	general	general	JJ
cord-010188-884d196k	7	8	properties	property	NNS
cord-010188-884d196k	7	9	of	of	IN
cord-010188-884d196k	7	10	RNA	RNA	NNP
cord-010188-884d196k	7	11	viruses	virus	NNS
cord-010188-884d196k	7	12	and	and	CC
cord-010188-884d196k	7	13	concentrates	concentrate	VBZ
cord-010188-884d196k	7	14	on	on	IN
cord-010188-884d196k	7	15	one	one	CD
cord-010188-884d196k	7	16	class	class	NN
cord-010188-884d196k	7	17	of	of	IN
cord-010188-884d196k	7	18	RNA	RNA	NNP
cord-010188-884d196k	7	19	viruses	virus	NNS
cord-010188-884d196k	7	20	—	—	:
cord-010188-884d196k	7	21	the	the	DT
cord-010188-884d196k	7	22	alphaviruses	alphavirus	NNS
cord-010188-884d196k	7	23	—	—	:
cord-010188-884d196k	7	24	and	and	CC
cord-010188-884d196k	7	25	their	-PRON-	PRP$
cord-010188-884d196k	7	26	potential	potential	NN
cord-010188-884d196k	7	27	as	as	IN
cord-010188-884d196k	7	28	expression	expression	NN
cord-010188-884d196k	7	29	vectors	vector	NNS
cord-010188-884d196k	7	30	.	.	.
cord-010188-884d196k	8	1	Virology	Virology	NNP
cord-010188-884d196k	8	2	is	be	VBZ
cord-010188-884d196k	8	3	celebrating	celebrate	VBG
cord-010188-884d196k	8	4	its	-PRON-	PRP$
cord-010188-884d196k	8	5	centennial	centennial	NN
cord-010188-884d196k	8	6	in	in	IN
cord-010188-884d196k	8	7	1992	1992	CD
cord-010188-884d196k	8	8	.	.	.
cord-010188-884d196k	9	1	The	the	DT
cord-010188-884d196k	9	2	existence	existence	NN
cord-010188-884d196k	9	3	of	of	IN
cord-010188-884d196k	9	4	a	a	DT
cord-010188-884d196k	9	5	filtrable	filtrable	JJ
cord-010188-884d196k	9	6	infectious	infectious	JJ
cord-010188-884d196k	9	7	agent	agent	NN
cord-010188-884d196k	9	8	was	be	VBD
cord-010188-884d196k	9	9	first	first	RB
cord-010188-884d196k	9	10	recognized	recognize	VBN
cord-010188-884d196k	9	11	100	100	CD
cord-010188-884d196k	9	12	years	year	NNS
cord-010188-884d196k	9	13	ago	ago	RB
cord-010188-884d196k	9	14	,	,	,
cord-010188-884d196k	9	15	when	when	WRB
cord-010188-884d196k	9	16	studies	study	NNS
cord-010188-884d196k	9	17	in	in	IN
cord-010188-884d196k	9	18	Russia	Russia	NNP
cord-010188-884d196k	9	19	and	and	CC
cord-010188-884d196k	9	20	The	the	DT
cord-010188-884d196k	9	21	Netherlands	Netherlands	NNP
cord-010188-884d196k	9	22	on	on	IN
cord-010188-884d196k	9	23	the	the	DT
cord-010188-884d196k	9	24	infectious	infectious	JJ
cord-010188-884d196k	9	25	agent	agent	NN
cord-010188-884d196k	9	26	that	that	WDT
cord-010188-884d196k	9	27	caused	cause	VBD
cord-010188-884d196k	9	28	the	the	DT
cord-010188-884d196k	9	29	mosaic	mosaic	JJ
cord-010188-884d196k	9	30	disease	disease	NN
cord-010188-884d196k	9	31	of	of	IN
cord-010188-884d196k	9	32	tobacco	tobacco	NN
cord-010188-884d196k	9	33	plants	plant	NNS
cord-010188-884d196k	9	34	led	lead	VBD
cord-010188-884d196k	9	35	to	to	IN
cord-010188-884d196k	9	36	the	the	DT
cord-010188-884d196k	9	37	concept	concept	NN
cord-010188-884d196k	9	38	of	of	IN
cord-010188-884d196k	9	39	infectious	infectious	JJ
cord-010188-884d196k	9	40	agents	agent	NNS
cord-010188-884d196k	9	41	that	that	WDT
cord-010188-884d196k	9	42	were	be	VBD
cord-010188-884d196k	9	43	smaller	small	JJR
cord-010188-884d196k	9	44	than	than	IN
cord-010188-884d196k	9	45	bacteria	bacteria	NNS
cord-010188-884d196k	9	46	1	1	CD
cord-010188-884d196k	9	47	.	.	.
cord-010188-884d196k	10	1	At	at	IN
cord-010188-884d196k	10	2	the	the	DT
cord-010188-884d196k	10	3	beginning	beginning	NN
cord-010188-884d196k	10	4	of	of	IN
cord-010188-884d196k	10	5	the	the	DT
cord-010188-884d196k	10	6	20th	20th	JJ
cord-010188-884d196k	10	7	century	century	NN
cord-010188-884d196k	10	8	,	,	,
cord-010188-884d196k	10	9	the	the	DT
cord-010188-884d196k	10	10	first	first	JJ
cord-010188-884d196k	10	11	human	human	JJ
cord-010188-884d196k	10	12	virus	virus	NN
cord-010188-884d196k	10	13	was	be	VBD
cord-010188-884d196k	10	14	discovered	discover	VBN
cord-010188-884d196k	10	15	yellow	yellow	JJ
cord-010188-884d196k	10	16	fever	fever	NN
cord-010188-884d196k	10	17	virus	virus	NN
cord-010188-884d196k	10	18	-and	-and	:
cord-010188-884d196k	10	19	for	for	IN
cord-010188-884d196k	10	20	much	much	JJ
cord-010188-884d196k	10	21	of	of	IN
cord-010188-884d196k	10	22	the	the	DT
cord-010188-884d196k	10	23	intervening	intervene	VBG
cord-010188-884d196k	10	24	time	time	NN
cord-010188-884d196k	10	25	the	the	DT
cord-010188-884d196k	10	26	study	study	NN
cord-010188-884d196k	10	27	of	of	IN
cord-010188-884d196k	10	28	viruses	virus	NNS
cord-010188-884d196k	10	29	has	have	VBZ
cord-010188-884d196k	10	30	been	be	VBN
cord-010188-884d196k	10	31	involved	involve	VBN
cord-010188-884d196k	10	32	primarily	primarily	RB
cord-010188-884d196k	10	33	in	in	IN
cord-010188-884d196k	10	34	the	the	DT
cord-010188-884d196k	10	35	identification	identification	NN
cord-010188-884d196k	10	36	and	and	CC
cord-010188-884d196k	10	37	characterization	characterization	NN
cord-010188-884d196k	10	38	of	of	IN
cord-010188-884d196k	10	39	viral	viral	JJ
cord-010188-884d196k	10	40	agents	agent	NNS
cord-010188-884d196k	10	41	that	that	WDT
cord-010188-884d196k	10	42	cause	cause	VBP
cord-010188-884d196k	10	43	disease	disease	NN
cord-010188-884d196k	10	44	.	.	.
cord-010188-884d196k	11	1	However	however	RB
cord-010188-884d196k	11	2	,	,	,
cord-010188-884d196k	11	3	viruses	virus	NNS
cord-010188-884d196k	11	4	are	be	VBP
cord-010188-884d196k	11	5	now	now	RB
cord-010188-884d196k	11	6	beginning	begin	VBG
cord-010188-884d196k	11	7	to	to	TO
cord-010188-884d196k	11	8	be	be	VB
cord-010188-884d196k	11	9	associated	associate	VBN
cord-010188-884d196k	11	10	not	not	RB
cord-010188-884d196k	11	11	only	only	RB
cord-010188-884d196k	11	12	with	with	IN
cord-010188-884d196k	11	13	the	the	DT
cord-010188-884d196k	11	14	cause	cause	NN
cord-010188-884d196k	11	15	of	of	IN
cord-010188-884d196k	11	16	disease	disease	NN
cord-010188-884d196k	11	17	,	,	,
cord-010188-884d196k	11	18	but	but	CC
cord-010188-884d196k	11	19	also	also	RB
cord-010188-884d196k	11	20	with	with	IN
cord-010188-884d196k	11	21	its	-PRON-	PRP$
cord-010188-884d196k	11	22	cure	cure	NN
cord-010188-884d196k	11	23	.	.	.
cord-010188-884d196k	12	1	Viruses	virus	NNS
cord-010188-884d196k	12	2	are	be	VBP
cord-010188-884d196k	12	3	being	be	VBG
cord-010188-884d196k	12	4	used	use	VBN
cord-010188-884d196k	12	5	as	as	IN
cord-010188-884d196k	12	6	vectors	vector	NNS
cord-010188-884d196k	12	7	for	for	IN
cord-010188-884d196k	12	8	the	the	DT
cord-010188-884d196k	12	9	introduction	introduction	NN
cord-010188-884d196k	12	10	of	of	IN
cord-010188-884d196k	12	11	heterologous	heterologous	JJ
cord-010188-884d196k	12	12	genes	gene	NNS
cord-010188-884d196k	12	13	into	into	IN
cord-010188-884d196k	12	14	cells	cell	NNS
cord-010188-884d196k	12	15	,	,	,
cord-010188-884d196k	12	16	which	which	WDT
cord-010188-884d196k	12	17	means	mean	VBZ
cord-010188-884d196k	12	18	that	that	IN
cord-010188-884d196k	12	19	they	-PRON-	PRP
cord-010188-884d196k	12	20	can	can	MD
cord-010188-884d196k	12	21	be	be	VB
cord-010188-884d196k	12	22	used	use	VBN
cord-010188-884d196k	12	23	for	for	IN
cord-010188-884d196k	12	24	the	the	DT
cord-010188-884d196k	12	25	delivery	delivery	NN
cord-010188-884d196k	12	26	of	of	IN
cord-010188-884d196k	12	27	therapeutic	therapeutic	JJ
cord-010188-884d196k	12	28	agents	agent	NNS
cord-010188-884d196k	12	29	into	into	IN
cord-010188-884d196k	12	30	cells	cell	NNS
cord-010188-884d196k	12	31	,	,	,
cord-010188-884d196k	12	32	as	as	RB
cord-010188-884d196k	12	33	well	well	RB
cord-010188-884d196k	12	34	as	as	IN
cord-010188-884d196k	12	35	for	for	IN
cord-010188-884d196k	12	36	gene	gene	NN
cord-010188-884d196k	12	37	therapy	therapy	NN
cord-010188-884d196k	12	38	.	.	.
cord-010188-884d196k	13	1	1KNA	1kna	LS
cord-010188-884d196k	14	1	viruses	virus	NNS
cord-010188-884d196k	14	2	infect	infect	VBP
cord-010188-884d196k	14	3	a	a	DT
cord-010188-884d196k	14	4	wide	wide	JJ
cord-010188-884d196k	14	5	range	range	NN
cord-010188-884d196k	14	6	of	of	IN
cord-010188-884d196k	14	7	organisms	organism	NNS
cord-010188-884d196k	14	8	,	,	,
cord-010188-884d196k	14	9	both	both	DT
cord-010188-884d196k	14	10	prokaryotic	prokaryotic	JJ
cord-010188-884d196k	14	11	and	and	CC
cord-010188-884d196k	14	12	eukaryotic	eukaryotic	JJ
cord-010188-884d196k	14	13	.	.	.
cord-010188-884d196k	15	1	An	an	DT
cord-010188-884d196k	15	2	important	important	JJ
cord-010188-884d196k	15	3	feature	feature	NN
cord-010188-884d196k	15	4	of	of	IN
cord-010188-884d196k	15	5	these	these	DT
cord-010188-884d196k	15	6	viruses	virus	NNS
cord-010188-884d196k	15	7	is	be	VBZ
cord-010188-884d196k	15	8	that	that	IN
cord-010188-884d196k	15	9	many	many	JJ
cord-010188-884d196k	15	10	of	of	IN
cord-010188-884d196k	15	11	them	-PRON-	PRP
cord-010188-884d196k	15	12	produce	produce	VBP
cord-010188-884d196k	15	13	high	high	JJ
cord-010188-884d196k	15	14	S.	S.	NNP
cord-010188-884d196k	15	15	Schlesinger	Schlesinger	NNP
cord-010188-884d196k	15	16	levels	level	NNS
cord-010188-884d196k	15	17	of	of	IN
cord-010188-884d196k	15	18	specific	specific	JJ
cord-010188-884d196k	15	19	viral	viral	JJ
cord-010188-884d196k	15	20	proteins	protein	NNS
cord-010188-884d196k	15	21	,	,	,
cord-010188-884d196k	15	22	which	which	WDT
cord-010188-884d196k	15	23	makes	make	VBZ
cord-010188-884d196k	15	24	them	-PRON-	PRP
cord-010188-884d196k	15	25	good	good	JJ
cord-010188-884d196k	15	26	candidates	candidate	NNS
cord-010188-884d196k	15	27	as	as	IN
cord-010188-884d196k	15	28	vectors	vector	NNS
cord-010188-884d196k	15	29	for	for	IN
cord-010188-884d196k	15	30	heterologous	heterologous	NNP
cord-010188-884d196k	15	31	gene	gene	NNP
cord-010188-884d196k	15	32	expression2	expression2	NNP
cord-010188-884d196k	15	33	,	,	,
cord-010188-884d196k	15	34	3	3	CD
cord-010188-884d196k	15	35	.	.	.
cord-010188-884d196k	16	1	To	to	TO
cord-010188-884d196k	16	2	use	use	VB
cord-010188-884d196k	16	3	the	the	DT
cord-010188-884d196k	16	4	RNA	RNA	NNP
cord-010188-884d196k	16	5	viral	viral	JJ
cord-010188-884d196k	16	6	genome	genome	NN
cord-010188-884d196k	16	7	as	as	IN
cord-010188-884d196k	16	8	a	a	DT
cord-010188-884d196k	16	9	vector	vector	NN
cord-010188-884d196k	16	10	necessitates	necessitate	VBZ
cord-010188-884d196k	16	11	first	first	RB
cord-010188-884d196k	16	12	converting	convert	VBG
cord-010188-884d196k	16	13	the	the	DT
cord-010188-884d196k	16	14	RNA	RNA	NNP
cord-010188-884d196k	16	15	genome	genome	NN
cord-010188-884d196k	16	16	into	into	IN
cord-010188-884d196k	16	17	a	a	DT
cord-010188-884d196k	16	18	genomic	genomic	JJ
cord-010188-884d196k	16	19	complementary	complementary	JJ
cord-010188-884d196k	16	20	DNA	dna	NN
cord-010188-884d196k	16	21	(	(	-LRB-
cord-010188-884d196k	16	22	eDNA	edna	NN
cord-010188-884d196k	16	23	)	)	-RRB-
cord-010188-884d196k	16	24	,	,	,
cord-010188-884d196k	16	25	which	which	WDT
cord-010188-884d196k	16	26	is	be	VBZ
cord-010188-884d196k	16	27	then	then	RB
cord-010188-884d196k	16	28	placed	place	VBN
cord-010188-884d196k	16	29	downstream	downstream	RB
cord-010188-884d196k	16	30	from	from	IN
cord-010188-884d196k	16	31	a	a	DT
cord-010188-884d196k	16	32	promoter	promoter	NN
cord-010188-884d196k	16	33	for	for	IN
cord-010188-884d196k	16	34	a	a	DT
cord-010188-884d196k	16	35	bacterial	bacterial	JJ
cord-010188-884d196k	16	36	or	or	CC
cord-010188-884d196k	16	37	bacteriophage	bacteriophage	NN
cord-010188-884d196k	16	38	DNA	dna	NN
cord-010188-884d196k	16	39	-	-	HYPH
cord-010188-884d196k	16	40	dependent	dependent	JJ
cord-010188-884d196k	16	41	RNA	RNA	NNP
cord-010188-884d196k	16	42	polymerase	polymerase	NN
cord-010188-884d196k	16	43	.	.	.
cord-010188-884d196k	17	1	Inserts	Inserts	NNP
cord-010188-884d196k	17	2	ofheterologous	ofheterologous	JJ
cord-010188-884d196k	17	3	DNA	DNA	NNP
cord-010188-884d196k	17	4	that	that	WDT
cord-010188-884d196k	17	5	encode	encode	VBP
cord-010188-884d196k	17	6	the	the	DT
cord-010188-884d196k	17	7	proteins	protein	NNS
cord-010188-884d196k	17	8	of	of	IN
cord-010188-884d196k	17	9	interest	interest	NN
cord-010188-884d196k	17	10	may	may	MD
cord-010188-884d196k	17	11	then	then	RB
cord-010188-884d196k	17	12	be	be	VB
cord-010188-884d196k	17	13	introduced	introduce	VBN
cord-010188-884d196k	17	14	into	into	IN
cord-010188-884d196k	17	15	the	the	DT
cord-010188-884d196k	17	16	eDNA	edna	NN
cord-010188-884d196k	17	17	downstream	downstream	NN
cord-010188-884d196k	17	18	from	from	IN
cord-010188-884d196k	17	19	a	a	DT
cord-010188-884d196k	17	20	strong	strong	JJ
cord-010188-884d196k	17	21	viral	viral	JJ
cord-010188-884d196k	17	22	promoter	promoter	NN
cord-010188-884d196k	17	23	by	by	IN
cord-010188-884d196k	17	24	standard	standard	JJ
cord-010188-884d196k	17	25	restriction	restriction	NN
cord-010188-884d196k	17	26	-	-	HYPH
cord-010188-884d196k	17	27	endonuclease	endonuclease	NN
cord-010188-884d196k	17	28	digestion	digestion	NN
cord-010188-884d196k	17	29	and	and	CC
cord-010188-884d196k	17	30	ligation	ligation	NN
cord-010188-884d196k	17	31	techniques	technique	NNS
cord-010188-884d196k	17	32	.	.	.
cord-010188-884d196k	18	1	The	the	DT
cord-010188-884d196k	18	2	whole	whole	JJ
cord-010188-884d196k	18	3	eDNA	edna	NN
cord-010188-884d196k	18	4	is	be	VBZ
cord-010188-884d196k	18	5	then	then	RB
cord-010188-884d196k	18	6	transcribed	transcribe	VBN
cord-010188-884d196k	18	7	in	in	IN
cord-010188-884d196k	18	8	vitro	vitro	FW
cord-010188-884d196k	18	9	,	,	,
cord-010188-884d196k	18	10	using	use	VBG
cord-010188-884d196k	18	11	the	the	DT
cord-010188-884d196k	18	12	RNA	RNA	NNP
cord-010188-884d196k	18	13	polymerase	polymerase	NN
cord-010188-884d196k	18	14	promoter	promoter	NN
cord-010188-884d196k	18	15	,	,	,
cord-010188-884d196k	18	16	into	into	IN
cord-010188-884d196k	18	17	the	the	DT
cord-010188-884d196k	18	18	genomic	genomic	JJ
cord-010188-884d196k	18	19	viral	viral	JJ
cord-010188-884d196k	18	20	RNA	RNA	NNP
cord-010188-884d196k	18	21	,	,	,
cord-010188-884d196k	18	22	which	which	WDT
cord-010188-884d196k	18	23	,	,	,
cord-010188-884d196k	18	24	in	in	IN
cord-010188-884d196k	18	25	many	many	JJ
cord-010188-884d196k	18	26	cases	case	NNS
cord-010188-884d196k	18	27	,	,	,
cord-010188-884d196k	18	28	can	can	MD
cord-010188-884d196k	18	29	be	be	VB
cord-010188-884d196k	18	30	directly	directly	RB
cord-010188-884d196k	18	31	transfected	transfecte	VBN
cord-010188-884d196k	18	32	into	into	IN
cord-010188-884d196k	18	33	cultured	cultured	JJ
cord-010188-884d196k	18	34	cells	cell	NNS
cord-010188-884d196k	18	35	,	,	,
cord-010188-884d196k	18	36	or	or	CC
cord-010188-884d196k	18	37	into	into	IN
cord-010188-884d196k	18	38	plants	plant	NNS
cord-010188-884d196k	18	39	or	or	CC
cord-010188-884d196k	18	40	animals	animal	NNS
cord-010188-884d196k	18	41	.	.	.
cord-010188-884d196k	19	1	Many	many	JJ
cord-010188-884d196k	19	2	RNA	RNA	NNP
cord-010188-884d196k	19	3	viruses	virus	NNS
cord-010188-884d196k	19	4	possess	possess	VBP
cord-010188-884d196k	19	5	relatively	relatively	RB
cord-010188-884d196k	19	6	small	small	JJ
cord-010188-884d196k	19	7	genomes	genome	NNS
cord-010188-884d196k	19	8	;	;	:
cord-010188-884d196k	19	9	cloning	clone	VBG
cord-010188-884d196k	19	10	of	of	IN
cord-010188-884d196k	19	11	the	the	DT
cord-010188-884d196k	19	12	recombinant	recombinant	JJ
cord-010188-884d196k	19	13	eDNA	edna	NN
cord-010188-884d196k	19	14	can	can	MD
cord-010188-884d196k	19	15	therefore	therefore	RB
cord-010188-884d196k	19	16	be	be	VB
cord-010188-884d196k	19	17	accomplished	accomplish	VBN
cord-010188-884d196k	19	18	in	in	IN
cord-010188-884d196k	19	19	only	only	RB
cord-010188-884d196k	19	20	a	a	DT
cord-010188-884d196k	19	21	few	few	JJ
cord-010188-884d196k	19	22	steps	step	NNS
cord-010188-884d196k	19	23	.	.	.
cord-010188-884d196k	20	1	Many	many	JJ
cord-010188-884d196k	20	2	of	of	IN
cord-010188-884d196k	20	3	the	the	DT
cord-010188-884d196k	20	4	RNA	RNA	NNP
cord-010188-884d196k	20	5	viruses	virus	NNS
cord-010188-884d196k	20	6	that	that	WDT
cord-010188-884d196k	20	7	infcct	infcct	NNP
cord-010188-884d196k	20	8	higher	high	JJR
cord-010188-884d196k	20	9	eukaryotic	eukaryotic	JJ
cord-010188-884d196k	20	10	organisms	organism	NNS
cord-010188-884d196k	20	11	replicate	replicate	VBP
cord-010188-884d196k	20	12	exclusively	exclusively	RB
cord-010188-884d196k	20	13	in	in	IN
cord-010188-884d196k	20	14	the	the	DT
cord-010188-884d196k	20	15	cytoplasm	cytoplasm	NN
cord-010188-884d196k	20	16	and	and	CC
cord-010188-884d196k	20	17	hence	hence	RB
cord-010188-884d196k	20	18	expression	expression	NN
cord-010188-884d196k	20	19	of	of	IN
cord-010188-884d196k	20	20	their	-PRON-	PRP$
cord-010188-884d196k	20	21	genes	gene	NNS
cord-010188-884d196k	20	22	will	will	MD
cord-010188-884d196k	20	23	be	be	VB
cord-010188-884d196k	20	24	independent	independent	JJ
cord-010188-884d196k	20	25	of	of	IN
cord-010188-884d196k	20	26	host	host	NN
cord-010188-884d196k	20	27	nuclear	nuclear	JJ
cord-010188-884d196k	20	28	functions	function	NNS
cord-010188-884d196k	20	29	and	and	CC
cord-010188-884d196k	20	30	will	will	MD
cord-010188-884d196k	20	31	not	not	RB
cord-010188-884d196k	20	32	involve	involve	VB
cord-010188-884d196k	20	33	any	any	DT
cord-010188-884d196k	20	34	splicing	splicing	NN
cord-010188-884d196k	20	35	.	.	.
cord-010188-884d196k	21	1	Not	not	RB
cord-010188-884d196k	21	2	all	all	DT
cord-010188-884d196k	21	3	RNA	RNA	NNP
cord-010188-884d196k	21	4	viruses	virus	NNS
cord-010188-884d196k	21	5	,	,	,
cord-010188-884d196k	21	6	however	however	RB
cord-010188-884d196k	21	7	,	,	,
cord-010188-884d196k	21	8	have	have	VBP
cord-010188-884d196k	21	9	the	the	DT
cord-010188-884d196k	21	10	same	same	JJ
cord-010188-884d196k	21	11	mode	mode	NN
cord-010188-884d196k	21	12	of	of	IN
cord-010188-884d196k	21	13	replication	replication	NN
cord-010188-884d196k	21	14	and	and	CC
cord-010188-884d196k	21	15	this	this	DT
cord-010188-884d196k	21	16	affects	affect	VBZ
cord-010188-884d196k	21	17	the	the	DT
cord-010188-884d196k	21	18	strategy	strategy	NN
cord-010188-884d196k	21	19	that	that	WDT
cord-010188-884d196k	21	20	must	must	MD
cord-010188-884d196k	21	21	reviews	review	NNS
cord-010188-884d196k	21	22	be	be	VB
cord-010188-884d196k	21	23	used	use	VBN
cord-010188-884d196k	21	24	in	in	IN
cord-010188-884d196k	21	25	engineering	engineer	VBG
cord-010188-884d196k	21	26	the	the	DT
cord-010188-884d196k	21	27	viral	viral	JJ
cord-010188-884d196k	21	28	genome	genome	NN
cord-010188-884d196k	21	29	to	to	TO
cord-010188-884d196k	21	30	obtain	obtain	VB
cord-010188-884d196k	21	31	expression	expression	NN
cord-010188-884d196k	21	32	of	of	IN
cord-010188-884d196k	21	33	the	the	DT
cord-010188-884d196k	21	34	heterologous	heterologous	JJ
cord-010188-884d196k	21	35	genes	gene	NNS
cord-010188-884d196k	21	36	.	.	.
cord-010188-884d196k	22	1	A	a	DT
cord-010188-884d196k	22	2	key	key	JJ
cord-010188-884d196k	22	3	feature	feature	NN
cord-010188-884d196k	22	4	which	which	WDT
cord-010188-884d196k	22	5	divides	divide	VBZ
cord-010188-884d196k	22	6	RNA	RNA	NNP
cord-010188-884d196k	22	7	viruses	virus	NNS
cord-010188-884d196k	22	8	into	into	IN
cord-010188-884d196k	22	9	two	two	CD
cord-010188-884d196k	22	10	categories	category	NNS
cord-010188-884d196k	22	11	is	be	VBZ
cord-010188-884d196k	22	12	the	the	DT
cord-010188-884d196k	22	13	'	'	``
cord-010188-884d196k	22	14	polarity	polarity	NN
cord-010188-884d196k	22	15	'	'	''
cord-010188-884d196k	22	16	of	of	IN
cord-010188-884d196k	22	17	the	the	DT
cord-010188-884d196k	22	18	RNA	RNA	NNP
cord-010188-884d196k	22	19	genome	genome	NN
cord-010188-884d196k	22	20	4	4	CD
cord-010188-884d196k	22	21	.	.	.
cord-010188-884d196k	23	1	RNA	RNA	NNP
cord-010188-884d196k	23	2	genomes	genome	NNS
cord-010188-884d196k	23	3	with	with	IN
cord-010188-884d196k	23	4	positive	positive	JJ
cord-010188-884d196k	23	5	polarity	polarity	NN
cord-010188-884d196k	23	6	are	be	VBP
cord-010188-884d196k	23	7	those	those	DT
cord-010188-884d196k	23	8	in	in	IN
cord-010188-884d196k	23	9	which	which	WDT
cord-010188-884d196k	23	10	the	the	DT
cord-010188-884d196k	23	11	genomic	genomic	JJ
cord-010188-884d196k	23	12	RNA	RNA	NNP
cord-010188-884d196k	23	13	functions	function	NNS
cord-010188-884d196k	23	14	as	as	IN
cord-010188-884d196k	23	15	a	a	DT
cord-010188-884d196k	23	16	mRNA	mrna	NN
cord-010188-884d196k	23	17	;	;	:
cord-010188-884d196k	23	18	the	the	DT
cord-010188-884d196k	23	19	naked	naked	JJ
cord-010188-884d196k	23	20	RNA	RNA	NNP
cord-010188-884d196k	23	21	genome	genome	NN
cord-010188-884d196k	23	22	added	add	VBN
cord-010188-884d196k	23	23	to	to	IN
cord-010188-884d196k	23	24	cells	cell	NNS
cord-010188-884d196k	23	25	is	be	VBZ
cord-010188-884d196k	23	26	infectious	infectious	JJ
cord-010188-884d196k	23	27	(	(	-LRB-
cord-010188-884d196k	23	28	i.e.	i.e.	FW
cord-010188-884d196k	23	29	it	-PRON-	PRP
cord-010188-884d196k	23	30	is	be	VBZ
cord-010188-884d196k	23	31	able	able	JJ
cord-010188-884d196k	23	32	to	to	TO
cord-010188-884d196k	23	33	initiate	initiate	VB
cord-010188-884d196k	23	34	the	the	DT
cord-010188-884d196k	23	35	steps	step	NNS
cord-010188-884d196k	23	36	leading	lead	VBG
cord-010188-884d196k	23	37	to	to	IN
cord-010188-884d196k	23	38	the	the	DT
cord-010188-884d196k	23	39	production	production	NN
cord-010188-884d196k	23	40	of	of	IN
cord-010188-884d196k	23	41	new	new	JJ
cord-010188-884d196k	23	42	virus	virus	NN
cord-010188-884d196k	23	43	progeny	progeny	NN
cord-010188-884d196k	23	44	)	)	-RRB-
cord-010188-884d196k	23	45	.	.	.
cord-010188-884d196k	24	1	RNA	RNA	NNP
cord-010188-884d196k	24	2	gcnomes	gcnomes	NNP
cord-010188-884d196k	24	3	in	in	IN
cord-010188-884d196k	24	4	which	which	WDT
cord-010188-884d196k	24	5	the	the	DT
cord-010188-884d196k	24	6	sequence	sequence	NN
cord-010188-884d196k	24	7	is	be	VBZ
cord-010188-884d196k	24	8	complementary	complementary	JJ
cord-010188-884d196k	24	9	to	to	IN
cord-010188-884d196k	24	10	the	the	DT
cord-010188-884d196k	24	11	mRNA	mrna	NN
cord-010188-884d196k	24	12	(	(	-LRB-
cord-010188-884d196k	24	13	i.e.	i.e.	FW
cord-010188-884d196k	24	14	negative	negative	JJ
cord-010188-884d196k	24	15	polarity	polarity	NN
cord-010188-884d196k	24	16	)	)	-RRB-
cord-010188-884d196k	24	17	must	must	MD
cord-010188-884d196k	24	18	be	be	VB
cord-010188-884d196k	24	19	transcribed	transcribe	VBN
cord-010188-884d196k	24	20	into	into	IN
cord-010188-884d196k	24	21	mRNA	mrna	NN
cord-010188-884d196k	24	22	before	before	IN
cord-010188-884d196k	24	23	translation	translation	NN
cord-010188-884d196k	24	24	to	to	TO
cord-010188-884d196k	24	25	yield	yield	VB
cord-010188-884d196k	24	26	protein	protein	NN
cord-010188-884d196k	24	27	can	can	MD
cord-010188-884d196k	24	28	occur	occur	VB
cord-010188-884d196k	24	29	.	.	.
cord-010188-884d196k	25	1	Negative	negative	JJ
cord-010188-884d196k	25	2	-	-	HYPH
cord-010188-884d196k	25	3	strand	strand	NN
cord-010188-884d196k	25	4	RNA	RNA	NNP
cord-010188-884d196k	25	5	viruses	virus	NNS
cord-010188-884d196k	25	6	contain	contain	VBP
cord-010188-884d196k	25	7	the	the	DT
cord-010188-884d196k	25	8	enzymes	enzyme	NNS
cord-010188-884d196k	25	9	required	require	VBN
cord-010188-884d196k	25	10	for	for	IN
cord-010188-884d196k	25	11	transcription	transcription	NN
cord-010188-884d196k	26	1	O	o	UH
cord-010188-884d196k	26	2	f	f	XX
cord-010188-884d196k	27	1	their	-PRON-	PRP$
cord-010188-884d196k	27	2	RNA	RNA	NNP
cord-010188-884d196k	27	3	within	within	IN
cord-010188-884d196k	27	4	the	the	DT
cord-010188-884d196k	27	5	virus	virus	NN
cord-010188-884d196k	27	6	particle	particle	NN
cord-010188-884d196k	27	7	.	.	.
cord-010188-884d196k	28	1	Although	although	IN
cord-010188-884d196k	28	2	this	this	DT
cord-010188-884d196k	28	3	makes	make	VBZ
cord-010188-884d196k	28	4	the	the	DT
cord-010188-884d196k	28	5	engineering	engineering	NN
cord-010188-884d196k	28	6	of	of	IN
cord-010188-884d196k	28	7	such	such	JJ
cord-010188-884d196k	28	8	genomes	genome	NNS
cord-010188-884d196k	28	9	more	more	RBR
cord-010188-884d196k	28	10	complex	complex	JJ
cord-010188-884d196k	28	11	,	,	,
cord-010188-884d196k	28	12	the	the	DT
cord-010188-884d196k	28	13	feasibility	feasibility	NN
cord-010188-884d196k	28	14	of	of	IN
cord-010188-884d196k	28	15	using	use	VBG
cord-010188-884d196k	28	16	these	these	DT
cord-010188-884d196k	28	17	genomes	genome	NNS
cord-010188-884d196k	28	18	to	to	TO
cord-010188-884d196k	28	19	express	express	VB
cord-010188-884d196k	28	20	heterologous	heterologous	JJ
cord-010188-884d196k	28	21	genes	gene	NNS
cord-010188-884d196k	28	22	has	have	VBZ
cord-010188-884d196k	28	23	been	be	VBN
cord-010188-884d196k	28	24	established	establish	VBN
cord-010188-884d196k	28	25	5,6	5,6	CD
cord-010188-884d196k	28	26	.	.	.
cord-010188-884d196k	29	1	Two	two	CD
cord-010188-884d196k	29	2	other	other	JJ
cord-010188-884d196k	29	3	characteristics	characteristic	NNS
cord-010188-884d196k	29	4	which	which	WDT
cord-010188-884d196k	29	5	vary	vary	VBP
cord-010188-884d196k	29	6	among	among	IN
cord-010188-884d196k	29	7	the	the	DT
cord-010188-884d196k	29	8	different	different	JJ
cord-010188-884d196k	29	9	RNA	rna	NN
cord-010188-884d196k	29	10	-	-	HYPH
cord-010188-884d196k	29	11	virus	virus	NN
cord-010188-884d196k	29	12	families	family	NNS
cord-010188-884d196k	29	13	and	and	CC
cord-010188-884d196k	29	14	are	be	VBP
cord-010188-884d196k	29	15	relevant	relevant	JJ
cord-010188-884d196k	29	16	to	to	IN
cord-010188-884d196k	29	17	their	-PRON-	PRP$
cord-010188-884d196k	29	18	potential	potential	JJ
cord-010188-884d196k	29	19	use	use	NN
cord-010188-884d196k	29	20	as	as	IN
cord-010188-884d196k	29	21	expression	expression	NN
cord-010188-884d196k	29	22	vectors	vector	NNS
cord-010188-884d196k	29	23	are	be	VBP
cord-010188-884d196k	29	24	:	:	:
cord-010188-884d196k	29	25	(	(	-LRB-
cord-010188-884d196k	29	26	1	1	LS
cord-010188-884d196k	29	27	)	)	-RRB-
cord-010188-884d196k	29	28	whether	whether	IN
cord-010188-884d196k	29	29	the	the	DT
cord-010188-884d196k	29	30	genome	genome	NN
cord-010188-884d196k	29	31	exists	exist	VBZ
cord-010188-884d196k	29	32	as	as	IN
cord-010188-884d196k	29	33	a	a	DT
cord-010188-884d196k	29	34	single	single	JJ
cord-010188-884d196k	29	35	molecule	molecule	NN
cord-010188-884d196k	29	36	of	of	IN
cord-010188-884d196k	29	37	RNA	RNA	NNP
cord-010188-884d196k	29	38	or	or	CC
cord-010188-884d196k	29	39	as	as	IN
cord-010188-884d196k	29	40	several	several	JJ
cord-010188-884d196k	29	41	molecules	molecule	NNS
cord-010188-884d196k	29	42	of	of	IN
cord-010188-884d196k	29	43	different	different	JJ
cord-010188-884d196k	29	44	structure	structure	NN
cord-010188-884d196k	29	45	(	(	-LRB-
cord-010188-884d196k	29	46	i.e.	i.e.	FW
cord-010188-884d196k	29	47	a	a	DT
cord-010188-884d196k	29	48	'	'	``
cord-010188-884d196k	29	49	segmented	segmented	JJ
cord-010188-884d196k	29	50	'	'	''
cord-010188-884d196k	29	51	genome	genome	NN
cord-010188-884d196k	29	52	)	)	-RRB-
cord-010188-884d196k	29	53	;	;	:
cord-010188-884d196k	29	54	and	and	CC
cord-010188-884d196k	29	55	(	(	-LRB-
cord-010188-884d196k	29	56	2	2	LS
cord-010188-884d196k	29	57	)	)	-RRB-
cord-010188-884d196k	29	58	how	how	WRB
cord-010188-884d196k	29	59	the	the	DT
cord-010188-884d196k	29	60	genome	genome	NN
cord-010188-884d196k	29	61	is	be	VBZ
cord-010188-884d196k	29	62	translated	translate	VBN
cord-010188-884d196k	29	63	.	.	.
cord-010188-884d196k	30	1	Many	many	JJ
cord-010188-884d196k	30	2	RNA	RNA	NNP
cord-010188-884d196k	30	3	viruses	virus	NNS
cord-010188-884d196k	30	4	have	have	VBP
cord-010188-884d196k	30	5	segmented	segment	VBN
cord-010188-884d196k	30	6	genomes	genome	NNS
cord-010188-884d196k	30	7	:	:	:
cord-010188-884d196k	30	8	examples	example	NNS
cord-010188-884d196k	30	9	include	include	VBP
cord-010188-884d196k	30	10	both	both	CC
cord-010188-884d196k	30	11	the	the	DT
cord-010188-884d196k	30	12	negative	negative	JJ
cord-010188-884d196k	30	13	-	-	HYPH
cord-010188-884d196k	30	14	strand	strand	NN
cord-010188-884d196k	30	15	RNA	RNA	NNP
cord-010188-884d196k	30	16	myxoviruses	myxovirus	NNS
cord-010188-884d196k	30	17	,	,	,
cord-010188-884d196k	30	18	most	most	RBS
cord-010188-884d196k	30	19	notably	notably	RB
cord-010188-884d196k	30	20	influenza	influenza	JJ
cord-010188-884d196k	30	21	virus	virus	NN
cord-010188-884d196k	30	22	;	;	:
cord-010188-884d196k	30	23	and	and	CC
cord-010188-884d196k	30	24	the	the	DT
cord-010188-884d196k	30	25	positive	positive	JJ
cord-010188-884d196k	30	26	-	-	HYPH
cord-010188-884d196k	30	27	strand	strand	NN
cord-010188-884d196k	30	28	RNA	RNA	NNP
cord-010188-884d196k	30	29	plant	plant	NN
cord-010188-884d196k	30	30	viruses	virus	NNS
cord-010188-884d196k	30	31	such	such	JJ
cord-010188-884d196k	30	32	as	as	IN
cord-010188-884d196k	30	33	the	the	DT
cord-010188-884d196k	30	34	bromovimses	bromovimse	NNS
cord-010188-884d196k	30	35	and	and	CC
cord-010188-884d196k	30	36	cucumoviruses	cucumoviruse	NNS
cord-010188-884d196k	30	37	.	.	.
cord-010188-884d196k	31	1	The	the	DT
cord-010188-884d196k	31	2	genome	genome	NN
cord-010188-884d196k	31	3	of	of	IN
cord-010188-884d196k	31	4	viruses	virus	NNS
cord-010188-884d196k	31	5	such	such	JJ
cord-010188-884d196k	31	6	as	as	IN
cord-010188-884d196k	31	7	togaviruses	togaviruses	NNP
cord-010188-884d196k	31	8	,	,	,
cord-010188-884d196k	31	9	picornaviruses	picornaviruses	NNP
cord-010188-884d196k	31	10	and	and	CC
cord-010188-884d196k	31	11	flaviviruses	flaviviruses	NNP
cord-010188-884d196k	31	12	is	be	VBZ
cord-010188-884d196k	31	13	a	a	DT
cord-010188-884d196k	31	14	single	single	JJ
cord-010188-884d196k	31	15	strand	strand	NN
cord-010188-884d196k	31	16	of	of	IN
cord-010188-884d196k	31	17	RNA	RNA	NNP
cord-010188-884d196k	31	18	of	of	IN
cord-010188-884d196k	31	19	positive	positive	JJ
cord-010188-884d196k	31	20	polari	polari	NNP
cord-010188-884d196k	31	21	W.	W.	NNP
cord-010188-884d196k	32	1	The	the	DT
cord-010188-884d196k	32	2	latter	latter	JJ
cord-010188-884d196k	32	3	two	two	CD
cord-010188-884d196k	32	4	virus	virus	NN
cord-010188-884d196k	32	5	families	family	NNS
cord-010188-884d196k	32	6	have	have	VBP
cord-010188-884d196k	32	7	RNA	RNA	NNP
cord-010188-884d196k	32	8	genomes	genome	NNS
cord-010188-884d196k	32	9	with	with	IN
cord-010188-884d196k	32	10	a	a	DT
cord-010188-884d196k	32	11	single	single	JJ
cord-010188-884d196k	32	12	open	open	JJ
cord-010188-884d196k	32	13	reading	reading	NN
cord-010188-884d196k	32	14	frame	frame	NN
cord-010188-884d196k	32	15	(	(	-LRB-
cord-010188-884d196k	32	16	ORF	ORF	NNP
cord-010188-884d196k	32	17	)	)	-RRB-
cord-010188-884d196k	32	18	,	,	,
cord-010188-884d196k	32	19	from	from	IN
cord-010188-884d196k	32	20	which	which	WDT
cord-010188-884d196k	32	21	the	the	DT
cord-010188-884d196k	32	22	individual	individual	JJ
cord-010188-884d196k	32	23	viral	viral	JJ
cord-010188-884d196k	32	24	proteins	protein	NNS
cord-010188-884d196k	32	25	are	be	VBP
cord-010188-884d196k	32	26	generated	generate	VBN
cord-010188-884d196k	32	27	by	by	IN
cord-010188-884d196k	32	28	co	co	NN
cord-010188-884d196k	32	29	-	-	JJ
cord-010188-884d196k	32	30	and	and	CC
cord-010188-884d196k	32	31	posttranslational	posttranslational	JJ
cord-010188-884d196k	32	32	proteolytic	proteolytic	JJ
cord-010188-884d196k	32	33	cleavages	cleavage	NNS
cord-010188-884d196k	32	34	.	.	.
cord-010188-884d196k	33	1	Insertions	insertion	NNS
cord-010188-884d196k	33	2	into	into	IN
cord-010188-884d196k	33	3	these	these	DT
cord-010188-884d196k	33	4	genomes	genome	NNS
cord-010188-884d196k	33	5	must	must	MD
cord-010188-884d196k	33	6	be	be	VB
cord-010188-884d196k	33	7	in	in	IN
cord-010188-884d196k	33	8	frame	frame	NN
cord-010188-884d196k	33	9	,	,	,
cord-010188-884d196k	33	10	and	and	CC
cord-010188-884d196k	33	11	must	must	MD
cord-010188-884d196k	33	12	permit	permit	VB
cord-010188-884d196k	33	13	the	the	DT
cord-010188-884d196k	33	14	correct	correct	JJ
cord-010188-884d196k	33	15	proteolytic	proteolytic	JJ
cord-010188-884d196k	33	16	processing	processing	NN
cord-010188-884d196k	33	17	.	.	.
cord-010188-884d196k	34	1	Efforts	effort	NNS
cord-010188-884d196k	34	2	to	to	TO
cord-010188-884d196k	34	3	insert	insert	VB
cord-010188-884d196k	34	4	small	small	JJ
cord-010188-884d196k	34	5	heterologous	heterologous	JJ
cord-010188-884d196k	34	6	epitopes	epitope	NNS
cord-010188-884d196k	34	7	into	into	IN
cord-010188-884d196k	34	8	the	the	DT
cord-010188-884d196k	34	9	picornavirus	picornavirus	NNP
cord-010188-884d196k	34	10	genome	genome	NN
cord-010188-884d196k	34	11	have	have	VBP
cord-010188-884d196k	34	12	met	meet	VBN
cord-010188-884d196k	34	13	with	with	IN
cord-010188-884d196k	34	14	success	success	NN
cord-010188-884d196k	34	15	7	7	CD
cord-010188-884d196k	34	16	.	.	.
cord-010188-884d196k	35	1	Togaviruses	Togaviruses	NNP
cord-010188-884d196k	35	2	,	,	,
cord-010188-884d196k	35	3	coronaviruses	coronaviruse	NNS
cord-010188-884d196k	35	4	and	and	CC
cord-010188-884d196k	35	5	the	the	DT
cord-010188-884d196k	35	6	plant	plant	NN
cord-010188-884d196k	35	7	viruses	virus	NNS
cord-010188-884d196k	35	8	,	,	,
cord-010188-884d196k	35	9	bromoviruses	bromoviruses	NNP
cord-010188-884d196k	35	10	and	and	CC
cord-010188-884d196k	35	11	cucumoviruses	cucumoviruses	NNPS
cord-010188-884d196k	35	12	,	,	,
cord-010188-884d196k	35	13	are	be	VBP
cord-010188-884d196k	35	14	examples	example	NNS
cord-010188-884d196k	35	15	of	of	IN
cord-010188-884d196k	35	16	virus	virus	NN
cord-010188-884d196k	35	17	families	family	NNS
cord-010188-884d196k	35	18	in	in	IN
cord-010188-884d196k	35	19	which	which	WDT
cord-010188-884d196k	35	20	translation	translation	NN
cord-010188-884d196k	35	21	of	of	IN
cord-010188-884d196k	35	22	the	the	DT
cord-010188-884d196k	35	23	nonstructural	nonstructural	JJ
cord-010188-884d196k	35	24	proteins	protein	NNS
cord-010188-884d196k	35	25	involved	involve	VBN
cord-010188-884d196k	35	26	in	in	IN
cord-010188-884d196k	35	27	replication	replication	NN
cord-010188-884d196k	35	28	,	,	,
cord-010188-884d196k	35	29	and	and	CC
cord-010188-884d196k	35	30	the	the	DT
cord-010188-884d196k	35	31	translation	translation	NN
cord-010188-884d196k	35	32	of	of	IN
cord-010188-884d196k	35	33	the	the	DT
cord-010188-884d196k	35	34	structural	structural	JJ
cord-010188-884d196k	35	35	proteins	protein	NNS
cord-010188-884d196k	35	36	essential	essential	JJ
cord-010188-884d196k	35	37	for	for	IN
cord-010188-884d196k	35	38	packaging	packaging	NN
cord-010188-884d196k	35	39	of	of	IN
cord-010188-884d196k	35	40	the	the	DT
cord-010188-884d196k	35	41	genome	genome	NN
cord-010188-884d196k	35	42	can	can	MD
cord-010188-884d196k	35	43	be	be	VB
cord-010188-884d196k	35	44	independent	independent	JJ
cord-010188-884d196k	35	45	.	.	.
cord-010188-884d196k	36	1	During	during	IN
cord-010188-884d196k	36	2	the	the	DT
cord-010188-884d196k	36	3	course	course	NN
cord-010188-884d196k	36	4	of	of	IN
cord-010188-884d196k	36	5	replication	replication	NN
cord-010188-884d196k	36	6	,	,	,
cord-010188-884d196k	36	7	the	the	DT
cord-010188-884d196k	36	8	infected	infected	JJ
cord-010188-884d196k	36	9	cell	cell	NN
cord-010188-884d196k	36	10	produces	produce	VBZ
cord-010188-884d196k	36	11	both	both	CC
cord-010188-884d196k	36	12	genomicand	genomicand	NNP
cord-010188-884d196k	36	13	subgenomic	subgenomic	JJ
cord-010188-884d196k	36	14	-	-	HYPH
cord-010188-884d196k	36	15	sized	sized	JJ
cord-010188-884d196k	36	16	mRNAs	mrna	NNS
cord-010188-884d196k	36	17	.	.	.
cord-010188-884d196k	37	1	The	the	DT
cord-010188-884d196k	37	2	genomic	genomic	JJ
cord-010188-884d196k	37	3	RNA	RNA	NNP
cord-010188-884d196k	37	4	is	be	VBZ
cord-010188-884d196k	37	5	translated	translate	VBN
cord-010188-884d196k	37	6	to	to	TO
cord-010188-884d196k	37	7	produce	produce	VB
cord-010188-884d196k	37	8	some	some	DT
cord-010188-884d196k	37	9	of	of	IN
cord-010188-884d196k	37	10	the	the	DT
cord-010188-884d196k	37	11	viral	viral	JJ
cord-010188-884d196k	37	12	-	-	HYPH
cord-010188-884d196k	37	13	specific	specific	JJ
cord-010188-884d196k	37	14	proteins	protein	NNS
cord-010188-884d196k	37	15	,	,	,
cord-010188-884d196k	37	16	but	but	CC
cord-010188-884d196k	37	17	the	the	DT
cord-010188-884d196k	37	18	other	other	JJ
cord-010188-884d196k	37	19	viral	viral	JJ
cord-010188-884d196k	37	20	proteins	protein	NNS
cord-010188-884d196k	37	21	are	be	VBP
cord-010188-884d196k	37	22	translated	translate	VBN
cord-010188-884d196k	37	23	from	from	IN
cord-010188-884d196k	37	24	subgenomic	subgenomic	NNP
cord-010188-884d196k	37	25	RNAs	RNAs	NNPS
cord-010188-884d196k	37	26	.	.	.
cord-010188-884d196k	38	1	Alphaviruses	alphavirus	NNS
cord-010188-884d196k	38	2	are	be	VBP
cord-010188-884d196k	38	3	the	the	DT
cord-010188-884d196k	38	4	major	major	JJ
cord-010188-884d196k	38	5	genus	genus	NN
cord-010188-884d196k	38	6	of	of	IN
cord-010188-884d196k	38	7	the	the	DT
cord-010188-884d196k	38	8	Togavirus	Togavirus	NNP
cord-010188-884d196k	38	9	family	family	NN
cord-010188-884d196k	38	10	8	8	CD
cord-010188-884d196k	38	11	.	.	.
cord-010188-884d196k	39	1	They	-PRON-	PRP
cord-010188-884d196k	39	2	include	include	VBP
cord-010188-884d196k	39	3	Sindbis	Sindbis	NNP
cord-010188-884d196k	39	4	virus	virus	NN
cord-010188-884d196k	39	5	,	,	,
cord-010188-884d196k	39	6	Semliki	Semliki	NNP
cord-010188-884d196k	39	7	Forest	Forest	NNP
cord-010188-884d196k	39	8	virus	virus	NN
cord-010188-884d196k	39	9	and	and	CC
cord-010188-884d196k	39	10	the	the	DT
cord-010188-884d196k	39	11	human	human	JJ
cord-010188-884d196k	39	12	pathogens	pathogen	NNS
cord-010188-884d196k	39	13	eastern	eastern	JJ
cord-010188-884d196k	39	14	and	and	CC
cord-010188-884d196k	39	15	western	western	JJ
cord-010188-884d196k	39	16	equine	equine	JJ
cord-010188-884d196k	39	17	encephalitis	encephalitis	NN
cord-010188-884d196k	39	18	viruses	virus	NNS
cord-010188-884d196k	39	19	.	.	.
cord-010188-884d196k	40	1	Sindbis	sindbis	NN
cord-010188-884d196k	40	2	virus	virus	NN
cord-010188-884d196k	40	3	and	and	CC
cord-010188-884d196k	40	4	Semliki	Semliki	NNP
cord-010188-884d196k	40	5	Forest	Forest	NNP
cord-010188-884d196k	40	6	vires	vis	NNS
cord-010188-884d196k	40	7	are	be	VBP
cord-010188-884d196k	40	8	best	well	RBS
cord-010188-884d196k	40	9	known	know	VBN
cord-010188-884d196k	40	10	as	as	IN
cord-010188-884d196k	40	11	valuable	valuable	JJ
cord-010188-884d196k	40	12	models	model	NNS
cord-010188-884d196k	40	13	for	for	IN
cord-010188-884d196k	40	14	molecular	molecular	JJ
cord-010188-884d196k	40	15	and	and	CC
cord-010188-884d196k	40	16	cell	cell	NN
cord-010188-884d196k	40	17	biology	biology	NN
cord-010188-884d196k	40	18	,	,	,
cord-010188-884d196k	40	19	and	and	CC
cord-010188-884d196k	40	20	it	-PRON-	PRP
cord-010188-884d196k	40	21	is	be	VBZ
cord-010188-884d196k	40	22	these	these	DT
cord-010188-884d196k	40	23	two	two	CD
cord-010188-884d196k	40	24	viruses	virus	NNS
cord-010188-884d196k	40	25	that	that	WDT
cord-010188-884d196k	40	26	are	be	VBP
cord-010188-884d196k	40	27	presently	presently	RB
cord-010188-884d196k	40	28	being	be	VBG
cord-010188-884d196k	40	29	developed	develop	VBN
cord-010188-884d196k	40	30	as	as	IN
cord-010188-884d196k	40	31	vectors	vector	NNS
cord-010188-884d196k	40	32	for	for	IN
cord-010188-884d196k	40	33	the	the	DT
cord-010188-884d196k	40	34	expression	expression	NN
cord-010188-884d196k	40	35	of	of	IN
cord-010188-884d196k	40	36	heterologous	heterologous	JJ
cord-010188-884d196k	40	37	genes	gene	NNS
cord-010188-884d196k	40	38	.	.	.
cord-010188-884d196k	41	1	The	the	DT
cord-010188-884d196k	41	2	alphavirus	alphavirus	NNP
cord-010188-884d196k	41	3	genome	genome	NN
cord-010188-884d196k	41	4	is	be	VBZ
cord-010188-884d196k	41	5	a	a	DT
cord-010188-884d196k	41	6	single	single	JJ
cord-010188-884d196k	41	7	-	-	HYPH
cord-010188-884d196k	41	8	strand	strand	NN
cord-010188-884d196k	41	9	RNA	rna	NN
cord-010188-884d196k	41	10	of	of	IN
cord-010188-884d196k	41	11	positive	positive	JJ
cord-010188-884d196k	41	12	polarity	polarity	NN
cord-010188-884d196k	41	13	of	of	IN
cord-010188-884d196k	41	14	~1.2	~1.2	CD
cord-010188-884d196k	41	15	x	x	SYM
cord-010188-884d196k	41	16	104	104	CD
cord-010188-884d196k	41	17	nucleotides	nucleotide	NNS
cord-010188-884d196k	41	18	,	,	,
cord-010188-884d196k	41	19	that	that	WDT
cord-010188-884d196k	41	20	is	be	VBZ
cord-010188-884d196k	41	21	capped	cap	VBN
cord-010188-884d196k	41	22	at	at	IN
cord-010188-884d196k	41	23	the	the	DT
cord-010188-884d196k	41	24	5	5	CD
cord-010188-884d196k	41	25	'	'	''
cord-010188-884d196k	41	26	terminus	terminus	NN
cord-010188-884d196k	41	27	and	and	CC
cord-010188-884d196k	41	28	polyadenylated	polyadenylate	VBD
cord-010188-884d196k	41	29	at	at	IN
cord-010188-884d196k	41	30	the	the	DT
cord-010188-884d196k	41	31	3	3	CD
cord-010188-884d196k	41	32	'	'	NN
cord-010188-884d196k	41	33	terminus	terminus	NN
cord-010188-884d196k	41	34	8,9	8,9	CD
cord-010188-884d196k	41	35	.	.	.
cord-010188-884d196k	42	1	The	the	DT
cord-010188-884d196k	42	2	genomic	genomic	JJ
cord-010188-884d196k	42	3	RNA	rna	NN
cord-010188-884d196k	42	4	is	be	VBZ
cord-010188-884d196k	42	5	referred	refer	VBN
cord-010188-884d196k	42	6	to	to	IN
cord-010188-884d196k	42	7	as	as	IN
cord-010188-884d196k	42	8	49S	49S	NNP
cord-010188-884d196k	42	9	RNA	RNA	NNP
cord-010188-884d196k	42	10	(	(	-LRB-
cord-010188-884d196k	42	11	on	on	IN
cord-010188-884d196k	42	12	the	the	DT
cord-010188-884d196k	42	13	basis	basis	NN
cord-010188-884d196k	42	14	of	of	IN
cord-010188-884d196k	42	15	its	-PRON-	PRP$
cord-010188-884d196k	42	16	sedimentation	sedimentation	NN
cord-010188-884d196k	42	17	coefficient	coefficient	NN
cord-010188-884d196k	42	18	)	)	-RRB-
cord-010188-884d196k	42	19	,	,	,
cord-010188-884d196k	42	20	and	and	CC
cord-010188-884d196k	42	21	is	be	VBZ
cord-010188-884d196k	42	22	encapsidated	encapsidate	VBN
cord-010188-884d196k	42	23	in	in	IN
cord-010188-884d196k	42	24	an	an	DT
cord-010188-884d196k	42	25	icosahedral	icosahedral	JJ
cord-010188-884d196k	42	26	protein	protein	NN
cord-010188-884d196k	42	27	shell	shell	NN
cord-010188-884d196k	42	28	which	which	WDT
cord-010188-884d196k	42	29	is	be	VBZ
cord-010188-884d196k	42	30	composed	compose	VBN
cord-010188-884d196k	42	31	of	of	IN
cord-010188-884d196k	42	32	a	a	DT
cord-010188-884d196k	42	33	singleprotein	singleprotein	NNP
cord-010188-884d196k	42	34	subunit	subunit	NN
cord-010188-884d196k	42	35	.	.	.
cord-010188-884d196k	43	1	This	this	DT
cord-010188-884d196k	43	2	shell	shell	NN
cord-010188-884d196k	43	3	is	be	VBZ
cord-010188-884d196k	43	4	surrounded	surround	VBN
cord-010188-884d196k	43	5	by	by	IN
cord-010188-884d196k	43	6	a	a	DT
cord-010188-884d196k	43	7	lipid	lipid	NN
cord-010188-884d196k	43	8	bilayer	bilayer	NN
cord-010188-884d196k	43	9	that	that	WDT
cord-010188-884d196k	43	10	contains	contain	VBZ
cord-010188-884d196k	43	11	two	two	CD
cord-010188-884d196k	43	12	transmembranal	transmembranal	JJ
cord-010188-884d196k	43	13	glycoproteins	glycoprotein	NNS
cord-010188-884d196k	43	14	,	,	,
cord-010188-884d196k	43	15	organized	organize	VBN
cord-010188-884d196k	43	16	as	as	IN
cord-010188-884d196k	43	17	trimers	trimer	NNS
cord-010188-884d196k	43	18	of	of	IN
cord-010188-884d196k	43	19	heterodimeric	heterodimeric	NNP
cord-010188-884d196k	43	20	subunits	subunit	NNS
cord-010188-884d196k	43	21	.	.	.
cord-010188-884d196k	44	1	As	as	IN
cord-010188-884d196k	44	2	many	many	JJ
cord-010188-884d196k	44	3	of	of	IN
cord-010188-884d196k	44	4	the	the	DT
cord-010188-884d196k	44	5	steps	step	NNS
cord-010188-884d196k	44	6	in	in	IN
cord-010188-884d196k	44	7	vims	vims	JJ
cord-010188-884d196k	44	8	replication	replication	NN
cord-010188-884d196k	44	9	and	and	CC
cord-010188-884d196k	44	10	maturation	maturation	NN
cord-010188-884d196k	44	11	utilize	utilize	VBP
cord-010188-884d196k	44	12	the	the	DT
cord-010188-884d196k	44	13	host	host	NN
cord-010188-884d196k	44	14	cellular	cellular	JJ
cord-010188-884d196k	44	15	machinery	machinery	NN
cord-010188-884d196k	44	16	,	,	,
cord-010188-884d196k	44	17	the	the	DT
cord-010188-884d196k	44	18	virus	virus	NN
cord-010188-884d196k	44	19	proteins	protein	NNS
cord-010188-884d196k	44	20	provide	provide	VBP
cord-010188-884d196k	44	21	markers	marker	NNS
cord-010188-884d196k	44	22	for	for	IN
cord-010188-884d196k	44	23	analysing	analyse	VBG
cord-010188-884d196k	44	24	the	the	DT
cord-010188-884d196k	44	25	synthesis	synthesis	NN
cord-010188-884d196k	44	26	and	and	CC
cord-010188-884d196k	44	27	modification	modification	NN
cord-010188-884d196k	44	28	of	of	IN
cord-010188-884d196k	44	29	membrane	membrane	NN
cord-010188-884d196k	44	30	glycoproteins	glycoprotein	NNS
cord-010188-884d196k	44	31	,	,	,
cord-010188-884d196k	44	32	and	and	CC
cord-010188-884d196k	44	33	the	the	DT
cord-010188-884d196k	44	34	localization	localization	NN
cord-010188-884d196k	44	35	of	of	IN
cord-010188-884d196k	44	36	proteins	protein	NNS
cord-010188-884d196k	44	37	to	to	IN
cord-010188-884d196k	44	38	the	the	DT
cord-010188-884d196k	44	39	plasma	plasma	NN
cord-010188-884d196k	44	40	membrane	membrane	NN
cord-010188-884d196k	44	41	.	.	.
cord-010188-884d196k	45	1	The	the	DT
cord-010188-884d196k	45	2	5	5	CD
cord-010188-884d196k	45	3	'	'	POS
cord-010188-884d196k	45	4	portion	portion	NN
cord-010188-884d196k	45	5	(	(	-LRB-
cord-010188-884d196k	45	6	approximately	approximately	RB
cord-010188-884d196k	45	7	two	two	CD
cord-010188-884d196k	45	8	-	-	HYPH
cord-010188-884d196k	45	9	thirds	third	NNS
cord-010188-884d196k	45	10	)	)	-RRB-
cord-010188-884d196k	45	11	of	of	IN
cord-010188-884d196k	45	12	the	the	DT
cord-010188-884d196k	45	13	alphavirus	alphavirus	NNP
cord-010188-884d196k	45	14	genome	genome	NNP
cord-010188-884d196k	45	15	contains	contain	VBZ
cord-010188-884d196k	45	16	the	the	DT
cord-010188-884d196k	45	17	genetic	genetic	JJ
cord-010188-884d196k	45	18	information	information	NN
cord-010188-884d196k	45	19	encoding	encode	VBG
cord-010188-884d196k	45	20	the	the	DT
cord-010188-884d196k	45	21	nonstructural	nonstructural	JJ
cord-010188-884d196k	45	22	viral	viral	JJ
cord-010188-884d196k	45	23	proteins	protein	NNS
cord-010188-884d196k	45	24	that	that	WDT
cord-010188-884d196k	45	25	are	be	VBP
cord-010188-884d196k	45	26	required	require	VBN
cord-010188-884d196k	45	27	for	for	IN
cord-010188-884d196k	45	28	transcription	transcription	NN
cord-010188-884d196k	45	29	and	and	CC
cord-010188-884d196k	45	30	replication	replication	NN
cord-010188-884d196k	45	31	of	of	IN
cord-010188-884d196k	45	32	the	the	DT
cord-010188-884d196k	45	33	viral	viral	JJ
cord-010188-884d196k	45	34	RNA	RNA	NNP
cord-010188-884d196k	45	35	8,9	8,9	CD
cord-010188-884d196k	45	36	.	.	.
cord-010188-884d196k	46	1	The	the	DT
cord-010188-884d196k	46	2	3	3	CD
cord-010188-884d196k	46	3	'	'	POS
cord-010188-884d196k	46	4	portion	portion	NN
cord-010188-884d196k	46	5	of	of	IN
cord-010188-884d196k	46	6	the	the	DT
cord-010188-884d196k	46	7	gene	gene	NN
cord-010188-884d196k	46	8	(	(	-LRB-
cord-010188-884d196k	46	9	approximately	approximately	RB
cord-010188-884d196k	46	10	one	one	CD
cord-010188-884d196k	46	11	-	-	HYPH
cord-010188-884d196k	46	12	third	third	NN
cord-010188-884d196k	46	13	)	)	-RRB-
cord-010188-884d196k	46	14	contains	contain	VBZ
cord-010188-884d196k	46	15	the	the	DT
cord-010188-884d196k	46	16	genes	gene	NNS
cord-010188-884d196k	46	17	encoding	encode	VBG
cord-010188-884d196k	46	18	the	the	DT
cord-010188-884d196k	46	19	viral	viral	JJ
cord-010188-884d196k	46	20	structural	structural	JJ
cord-010188-884d196k	46	21	proteins	protein	NNS
cord-010188-884d196k	46	22	-the	-the	WDT
cord-010188-884d196k	46	23	capsid	capsid	NN
cord-010188-884d196k	46	24	protein	protein	NN
cord-010188-884d196k	46	25	,	,	,
cord-010188-884d196k	46	26	a	a	DT
cord-010188-884d196k	46	27	hydrophobic	hydrophobic	JJ
cord-010188-884d196k	46	28	6kDa	6kda	CD
cord-010188-884d196k	46	29	protein	protein	NN
cord-010188-884d196k	46	30	and	and	CC
cord-010188-884d196k	46	31	the	the	DT
cord-010188-884d196k	46	32	two	two	CD
cord-010188-884d196k	46	33	viral	viral	JJ
cord-010188-884d196k	46	34	envelope	envelope	NN
cord-010188-884d196k	46	35	glycoproteins	glycoprotein	NNS
cord-010188-884d196k	46	36	.	.	.
cord-010188-884d196k	47	1	The	the	DT
cord-010188-884d196k	47	2	replication	replication	NN
cord-010188-884d196k	47	3	cycle	cycle	NN
cord-010188-884d196k	47	4	of	of	IN
cord-010188-884d196k	47	5	the	the	DT
cord-010188-884d196k	47	6	alphaviruses	alphaviruses	NNP
cord-010188-884d196k	47	7	is	be	VBZ
cord-010188-884d196k	47	8	shown	show	VBN
cord-010188-884d196k	47	9	schematically	schematically	RB
cord-010188-884d196k	47	10	in	in	IN
cord-010188-884d196k	47	11	Fig	Fig	NNP
cord-010188-884d196k	47	12	.	.	.
cord-010188-884d196k	48	1	1	1	LS
cord-010188-884d196k	48	2	.	.	.
cord-010188-884d196k	49	1	Following	follow	VBG
cord-010188-884d196k	49	2	attachment	attachment	NN
cord-010188-884d196k	49	3	of	of	IN
cord-010188-884d196k	49	4	the	the	DT
cord-010188-884d196k	49	5	virus	virus	NN
cord-010188-884d196k	49	6	to	to	IN
cord-010188-884d196k	49	7	the	the	DT
cord-010188-884d196k	49	8	cell	cell	NN
cord-010188-884d196k	49	9	surface	surface	NN
cord-010188-884d196k	49	10	,	,	,
cord-010188-884d196k	49	11	entry	entry	NN
cord-010188-884d196k	49	12	is	be	VBZ
cord-010188-884d196k	49	13	effected	effect	VBN
cord-010188-884d196k	49	14	by	by	IN
cord-010188-884d196k	49	15	receptormediated	receptormediated	JJ
cord-010188-884d196k	49	16	endocytosis	endocytosis	NN
cord-010188-884d196k	49	17	.	.	.
cord-010188-884d196k	50	1	Fusion	fusion	NN
cord-010188-884d196k	50	2	of	of	IN
cord-010188-884d196k	50	3	the	the	DT
cord-010188-884d196k	50	4	virus	virus	NN
cord-010188-884d196k	50	5	membrane	membrane	NN
cord-010188-884d196k	50	6	with	with	IN
cord-010188-884d196k	50	7	the	the	DT
cord-010188-884d196k	50	8	endosomal	endosomal	NNP
cord-010188-884d196k	50	9	membrane	membrane	NN
cord-010188-884d196k	50	10	releases	release	VBZ
cord-010188-884d196k	50	11	the	the	DT
cord-010188-884d196k	50	12	viral	viral	JJ
cord-010188-884d196k	50	13	nucleocapsid	nucleocapsid	NN
cord-010188-884d196k	50	14	into	into	IN
cord-010188-884d196k	50	15	the	the	DT
cord-010188-884d196k	50	16	cytoplasm	cytoplasm	NN
cord-010188-884d196k	50	17	,	,	,
cord-010188-884d196k	50	18	where	where	WRB
cord-010188-884d196k	50	19	translation	translation	NN
cord-010188-884d196k	50	20	of	of	IN
cord-010188-884d196k	50	21	the	the	DT
cord-010188-884d196k	50	22	genomic	genomic	JJ
cord-010188-884d196k	50	23	RNA	rna	NN
cord-010188-884d196k	50	24	occurs	occur	VBZ
cord-010188-884d196k	50	25	.	.	.
cord-010188-884d196k	51	1	Only	only	RB
cord-010188-884d196k	51	2	the	the	DT
cord-010188-884d196k	51	3	nonstructural	nonstructural	JJ
cord-010188-884d196k	51	4	viral	viral	JJ
cord-010188-884d196k	51	5	proteins	protein	NNS
cord-010188-884d196k	51	6	are	be	VBP
cord-010188-884d196k	51	7	translated	translate	VBN
cord-010188-884d196k	51	8	from	from	IN
cord-010188-884d196k	51	9	the	the	DT
cord-010188-884d196k	51	10	genomic	genomic	JJ
cord-010188-884d196k	51	11	RNA	rna	NN
cord-010188-884d196k	51	12	.	.	.
cord-010188-884d196k	52	1	There	there	EX
cord-010188-884d196k	52	2	is	be	VBZ
cord-010188-884d196k	52	3	a	a	DT
cord-010188-884d196k	52	4	single	single	JJ
cord-010188-884d196k	52	5	ORF	ORF	NNP
cord-010188-884d196k	52	6	and	and	CC
cord-010188-884d196k	52	7	the	the	DT
cord-010188-884d196k	52	8	resulting	result	VBG
cord-010188-884d196k	52	9	polyprotein	polyprotein	NN
cord-010188-884d196k	52	10	is	be	VBZ
cord-010188-884d196k	52	11	co	co	JJ
cord-010188-884d196k	52	12	-	-	JJ
cord-010188-884d196k	52	13	and	and	CC
cord-010188-884d196k	52	14	post	post	VB
cord-010188-884d196k	52	15	-	-	NN
cord-010188-884d196k	52	16	translationally	translationally	RB
cord-010188-884d196k	52	17	cleaved	cleave	VBN
cord-010188-884d196k	52	18	to	to	TO
cord-010188-884d196k	52	19	form	form	VB
cord-010188-884d196k	52	20	four	four	CD
cord-010188-884d196k	52	21	polypeptides	polypeptide	NNS
cord-010188-884d196k	52	22	.	.	.
cord-010188-884d196k	53	1	These	these	DT
cord-010188-884d196k	53	2	function	function	VBP
cord-010188-884d196k	53	3	together	together	RB
cord-010188-884d196k	53	4	in	in	IN
cord-010188-884d196k	53	5	a	a	DT
cord-010188-884d196k	53	6	replication	replication	NN
cord-010188-884d196k	53	7	complex	complex	NN
cord-010188-884d196k	53	8	which	which	WDT
cord-010188-884d196k	53	9	is	be	VBZ
cord-010188-884d196k	53	10	required	require	VBN
cord-010188-884d196k	53	11	for	for	IN
cord-010188-884d196k	53	12	the	the	DT
cord-010188-884d196k	53	13	synthesis	synthesis	NN
cord-010188-884d196k	53	14	of	of	IN
cord-010188-884d196k	53	15	the	the	DT
cord-010188-884d196k	53	16	complementary	complementary	JJ
cord-010188-884d196k	53	17	,	,	,
cord-010188-884d196k	53	18	negative	negative	JJ
cord-010188-884d196k	53	19	RNA	RNA	NNP
cord-010188-884d196k	53	20	strand	strand	NN
cord-010188-884d196k	53	21	.	.	.
cord-010188-884d196k	54	1	The	the	DT
cord-010188-884d196k	54	2	newly	newly	RB
cord-010188-884d196k	54	3	synthesized	synthesize	VBN
cord-010188-884d196k	54	4	negative	negative	JJ
cord-010188-884d196k	54	5	RNA	RNA	NNP
cord-010188-884d196k	54	6	strand	strand	NN
cord-010188-884d196k	54	7	serves	serve	VBZ
cord-010188-884d196k	54	8	as	as	IN
cord-010188-884d196k	54	9	a	a	DT
cord-010188-884d196k	54	10	template	template	NN
cord-010188-884d196k	54	11	for	for	IN
cord-010188-884d196k	54	12	the	the	DT
cord-010188-884d196k	54	13	synthesis	synthesis	NN
cord-010188-884d196k	54	14	of	of	IN
cord-010188-884d196k	54	15	more	more	JJR
cord-010188-884d196k	54	16	virion	virion	NN
cord-010188-884d196k	54	17	RNA	RNA	NNP
cord-010188-884d196k	54	18	,	,	,
cord-010188-884d196k	54	19	and	and	CC
cord-010188-884d196k	54	20	also	also	RB
cord-010188-884d196k	54	21	for	for	IN
cord-010188-884d196k	54	22	the	the	DT
cord-010188-884d196k	54	23	transcription	transcription	NN
cord-010188-884d196k	54	24	ofa	ofa	NN
cord-010188-884d196k	54	25	subgenomic	subgenomic	NNP
cord-010188-884d196k	54	26	(	(	-LRB-
cord-010188-884d196k	54	27	26S	26S	NNP
cord-010188-884d196k	54	28	)	)	-RRB-
cord-010188-884d196k	54	29	RNA	RNA	NNP
cord-010188-884d196k	54	30	which	which	WDT
cord-010188-884d196k	54	31	may	may	MD
cord-010188-884d196k	54	32	then	then	RB
cord-010188-884d196k	54	33	be	be	VB
cord-010188-884d196k	54	34	translated	translate	VBN
cord-010188-884d196k	54	35	to	to	TO
cord-010188-884d196k	54	36	yield	yield	VB
cord-010188-884d196k	54	37	the	the	DT
cord-010188-884d196k	54	38	structural	structural	JJ
cord-010188-884d196k	54	39	proteins	protein	NNS
cord-010188-884d196k	54	40	.	.	.
cord-010188-884d196k	55	1	The	the	DT
cord-010188-884d196k	55	2	nucleotides	nucleotide	NNS
cord-010188-884d196k	55	3	spanning	span	VBG
cord-010188-884d196k	55	4	the	the	DT
cord-010188-884d196k	55	5	junction	junction	NN
cord-010188-884d196k	55	6	between	between	IN
cord-010188-884d196k	55	7	the	the	DT
cord-010188-884d196k	55	8	genes	gene	NNS
cord-010188-884d196k	55	9	for	for	IN
cord-010188-884d196k	55	10	the	the	DT
cord-010188-884d196k	55	11	nonstructural	nonstructural	JJ
cord-010188-884d196k	55	12	protein	protein	NN
cord-010188-884d196k	55	13	and	and	CC
cord-010188-884d196k	55	14	those	those	DT
cord-010188-884d196k	55	15	for	for	IN
cord-010188-884d196k	55	16	the	the	DT
cord-010188-884d196k	55	17	structural	structural	JJ
cord-010188-884d196k	55	18	proteins	protein	NNS
cord-010188-884d196k	55	19	on	on	IN
cord-010188-884d196k	55	20	the	the	DT
cord-010188-884d196k	55	21	negative	negative	JJ
cord-010188-884d196k	55	22	RNA	RNA	NNP
cord-010188-884d196k	55	23	strand	strand	NN
cord-010188-884d196k	55	24	serve	serve	VB
cord-010188-884d196k	55	25	as	as	IN
cord-010188-884d196k	55	26	the	the	DT
cord-010188-884d196k	55	27	promoter	promoter	NN
cord-010188-884d196k	55	28	for	for	IN
cord-010188-884d196k	55	29	transcription	transcription	NN
cord-010188-884d196k	55	30	of	of	IN
cord-010188-884d196k	55	31	the	the	DT
cord-010188-884d196k	55	32	subgenomic	subgenomic	NNP
cord-010188-884d196k	55	33	RNA	RNA	NNP
cord-010188-884d196k	55	34	1	1	CD
cord-010188-884d196k	55	35	°	°	NN
cord-010188-884d196k	55	36	,11	,11	NN
cord-010188-884d196k	55	37	.	.	.
cord-010188-884d196k	56	1	The	the	DT
cord-010188-884d196k	56	2	minimal	minimal	JJ
cord-010188-884d196k	56	3	sequence	sequence	NN
cord-010188-884d196k	56	4	required	require	VBN
cord-010188-884d196k	56	5	to	to	TO
cord-010188-884d196k	56	6	initiate	initiate	VB
cord-010188-884d196k	56	7	transcription	transcription	NN
cord-010188-884d196k	56	8	(	(	-LRB-
cord-010188-884d196k	56	9	19	19	CD
cord-010188-884d196k	56	10	nucleotides	nucleotide	NNS
cord-010188-884d196k	56	11	upstream	upstream	RB
cord-010188-884d196k	56	12	and	and	CC
cord-010188-884d196k	56	13	five	five	CD
cord-010188-884d196k	56	14	nucleotides	nucleotide	NNS
cord-010188-884d196k	56	15	downstream	downstream	RB
cord-010188-884d196k	56	16	from	from	IN
cord-010188-884d196k	56	17	the	the	DT
cord-010188-884d196k	56	18	start	start	NN
cord-010188-884d196k	56	19	of	of	IN
cord-010188-884d196k	56	20	the	the	DT
cord-010188-884d196k	56	21	subgenomic	subgenomic	NNP
cord-010188-884d196k	56	22	RNA	RNA	NNP
cord-010188-884d196k	56	23	)	)	-RRB-
cord-010188-884d196k	56	24	is	be	VBZ
cord-010188-884d196k	56	25	located	locate	VBN
cord-010188-884d196k	56	26	within	within	IN
cord-010188-884d196k	56	27	a	a	DT
cord-010188-884d196k	56	28	region	region	NN
cord-010188-884d196k	56	29	that	that	WDT
cord-010188-884d196k	56	30	is	be	VBZ
cord-010188-884d196k	56	31	highly	highly	RB
cord-010188-884d196k	56	32	conserved	conserve	VBN
cord-010188-884d196k	56	33	among	among	IN
cord-010188-884d196k	56	34	alphaviruses	alphavirus	NNS
cord-010188-884d196k	56	35	9	9	CD
cord-010188-884d196k	56	36	11	11	CD
cord-010188-884d196k	56	37	.	.	.
cord-010188-884d196k	57	1	If	if	IN
cord-010188-884d196k	57	2	regions	region	NNS
cord-010188-884d196k	57	3	flanking	flank	VBG
cord-010188-884d196k	57	4	the	the	DT
cord-010188-884d196k	57	5	minimal	minimal	JJ
cord-010188-884d196k	57	6	promoter	promoter	NN
cord-010188-884d196k	57	7	are	be	VBP
cord-010188-884d196k	57	8	also	also	RB
cord-010188-884d196k	57	9	present	present	JJ
cord-010188-884d196k	57	10	,	,	,
cord-010188-884d196k	57	11	the	the	DT
cord-010188-884d196k	57	12	level	level	NN
cord-010188-884d196k	57	13	of	of	IN
cord-010188-884d196k	57	14	transcription	transcription	NN
cord-010188-884d196k	57	15	of	of	IN
cord-010188-884d196k	57	16	the	the	DT
cord-010188-884d196k	57	17	subgenomic	subgenomic	NNP
cord-010188-884d196k	57	18	RNA	RNA	NNP
cord-010188-884d196k	57	19	is	be	VBZ
cord-010188-884d196k	57	20	increased	increase	VBN
cord-010188-884d196k	57	21	.	.	.
cord-010188-884d196k	58	1	The	the	DT
cord-010188-884d196k	58	2	viral	viral	JJ
cord-010188-884d196k	58	3	structural	structural	JJ
cord-010188-884d196k	58	4	proteins	protein	NNS
cord-010188-884d196k	58	5	translated	translate	VBN
cord-010188-884d196k	58	6	from	from	IN
cord-010188-884d196k	58	7	26S	26S	NNP
cord-010188-884d196k	58	8	RNA	RNA	NNP
cord-010188-884d196k	58	9	are	be	VBP
cord-010188-884d196k	58	10	also	also	RB
cord-010188-884d196k	58	11	synthesized	synthesize	VBN
cord-010188-884d196k	58	12	as	as	IN
cord-010188-884d196k	58	13	a	a	DT
cord-010188-884d196k	58	14	polyprotein	polyprotein	NN
cord-010188-884d196k	58	15	,	,	,
cord-010188-884d196k	58	16	with	with	IN
cord-010188-884d196k	58	17	the	the	DT
cord-010188-884d196k	58	18	N	n	CD
cord-010188-884d196k	58	19	-	-	HYPH
cord-010188-884d196k	58	20	terminal	terminal	NN
cord-010188-884d196k	58	21	capsid	capsid	NN
cord-010188-884d196k	58	22	protein	protein	NN
cord-010188-884d196k	58	23	functioning	function	VBG
cord-010188-884d196k	58	24	as	as	IN
cord-010188-884d196k	58	25	an	an	DT
cord-010188-884d196k	58	26	autoprotease	autoprotease	NN
cord-010188-884d196k	58	27	to	to	TO
cord-010188-884d196k	58	28	cleave	cleave	VB
cord-010188-884d196k	58	29	itself	-PRON-	PRP
cord-010188-884d196k	58	30	from	from	IN
cord-010188-884d196k	58	31	the	the	DT
cord-010188-884d196k	58	32	nascent	nascent	JJ
cord-010188-884d196k	58	33	polypeptide	polypeptide	NN
cord-010188-884d196k	58	34	.	.	.
cord-010188-884d196k	59	1	The	the	DT
cord-010188-884d196k	59	2	capsid	capsid	NN
cord-010188-884d196k	59	3	protein	protein	NN
cord-010188-884d196k	59	4	can	can	MD
cord-010188-884d196k	59	5	then	then	RB
cord-010188-884d196k	59	6	interact	interact	VB
cord-010188-884d196k	59	7	with	with	IN
cord-010188-884d196k	59	8	the	the	DT
cord-010188-884d196k	59	9	genomic	genomic	JJ
cord-010188-884d196k	59	10	RNA	RNA	NNP
cord-010188-884d196k	59	11	to	to	TO
cord-010188-884d196k	59	12	form	form	VB
cord-010188-884d196k	59	13	the	the	DT
cord-010188-884d196k	59	14	nucleocapsid	nucleocapsid	NN
cord-010188-884d196k	59	15	.	.	.
cord-010188-884d196k	60	1	There	there	EX
cord-010188-884d196k	60	2	is	be	VBZ
cord-010188-884d196k	60	3	a	a	DT
cord-010188-884d196k	60	4	specific	specific	JJ
cord-010188-884d196k	60	5	recognition	recognition	NN
cord-010188-884d196k	60	6	signal	signal	NN
cord-010188-884d196k	60	7	located	locate	VBN
cord-010188-884d196k	60	8	near	near	IN
cord-010188-884d196k	60	9	the	the	DT
cord-010188-884d196k	60	10	5	5	CD
cord-010188-884d196k	60	11	'	'	''
cord-010188-884d196k	60	12	terminus	terminus	NN
cord-010188-884d196k	60	13	of	of	IN
cord-010188-884d196k	60	14	the	the	DT
cord-010188-884d196k	60	15	RNA	RNA	NNP
cord-010188-884d196k	60	16	that	that	WDT
cord-010188-884d196k	60	17	serves	serve	VBZ
cord-010188-884d196k	60	18	as	as	IN
cord-010188-884d196k	60	19	the	the	DT
cord-010188-884d196k	60	20	nucleation	nucleation	NN
cord-010188-884d196k	60	21	site	site	NN
cord-010188-884d196k	60	22	for	for	IN
cord-010188-884d196k	60	23	encapsidation	encapsidation	NN
cord-010188-884d196k	60	24	12	12	CD
cord-010188-884d196k	60	25	and	and	CC
cord-010188-884d196k	60	26	thus	thus	RB
cord-010188-884d196k	60	27	ensures	ensure	VBZ
cord-010188-884d196k	60	28	that	that	IN
cord-010188-884d196k	60	29	the	the	DT
cord-010188-884d196k	60	30	genomic	genomic	NN
cord-010188-884d196k	60	31	,	,	,
cord-010188-884d196k	60	32	but	but	CC
cord-010188-884d196k	60	33	not	not	RB
cord-010188-884d196k	60	34	the	the	DT
cord-010188-884d196k	60	35	subgenomic	subgenomic	NNP
cord-010188-884d196k	60	36	RNA	RNA	NNP
cord-010188-884d196k	60	37	,	,	,
cord-010188-884d196k	60	38	is	be	VBZ
cord-010188-884d196k	60	39	packaged	package	VBN
cord-010188-884d196k	60	40	.	.	.
cord-010188-884d196k	61	1	The	the	DT
cord-010188-884d196k	61	2	viral	viral	JJ
cord-010188-884d196k	61	3	glycoproteins	glycoprotein	NNS
cord-010188-884d196k	61	4	,	,	,
cord-010188-884d196k	61	5	synthesized	synthesize	VBN
cord-010188-884d196k	61	6	on	on	IN
cord-010188-884d196k	61	7	membrane	membrane	NN
cord-010188-884d196k	61	8	-	-	HYPH
cord-010188-884d196k	61	9	bound	bind	VBN
cord-010188-884d196k	61	10	ribosomes	ribosome	NNS
cord-010188-884d196k	61	11	,	,	,
cord-010188-884d196k	61	12	are	be	VBP
cord-010188-884d196k	61	13	transported	transport	VBN
cord-010188-884d196k	61	14	through	through	IN
cord-010188-884d196k	61	15	the	the	DT
cord-010188-884d196k	61	16	host	host	NN
cord-010188-884d196k	61	17	-	-	HYPH
cord-010188-884d196k	61	18	cell	cell	NN
cord-010188-884d196k	61	19	Golgi	Golgi	NNP
cord-010188-884d196k	61	20	network	network	NN
cord-010188-884d196k	61	21	to	to	IN
cord-010188-884d196k	61	22	the	the	DT
cord-010188-884d196k	61	23	plasma	plasma	NN
cord-010188-884d196k	61	24	membrane	membrane	NN
cord-010188-884d196k	61	25	of	of	IN
cord-010188-884d196k	61	26	the	the	DT
cord-010188-884d196k	61	27	infected	infected	JJ
cord-010188-884d196k	61	28	cell	cell	NN
cord-010188-884d196k	61	29	.	.	.
cord-010188-884d196k	62	1	Finally	finally	RB
cord-010188-884d196k	62	2	,	,	,
cord-010188-884d196k	62	3	interaction	interaction	NN
cord-010188-884d196k	62	4	of	of	IN
cord-010188-884d196k	62	5	the	the	DT
cord-010188-884d196k	62	6	nucleocapsid	nucleocapsid	NN
cord-010188-884d196k	62	7	with	with	IN
cord-010188-884d196k	62	8	the	the	DT
cord-010188-884d196k	62	9	cytoplasmic	cytoplasmic	JJ
cord-010188-884d196k	62	10	tail	tail	NN
cord-010188-884d196k	62	11	of	of	IN
cord-010188-884d196k	62	12	a	a	DT
cord-010188-884d196k	62	13	viral	viral	JJ
cord-010188-884d196k	62	14	glycoprotein	glycoprotein	NN
cord-010188-884d196k	62	15	leads	lead	VBZ
cord-010188-884d196k	62	16	to	to	IN
cord-010188-884d196k	62	17	budding	bud	VBG
cord-010188-884d196k	62	18	of	of	IN
cord-010188-884d196k	62	19	the	the	DT
cord-010188-884d196k	62	20	virus	virus	NN
cord-010188-884d196k	62	21	particle	particle	NN
cord-010188-884d196k	62	22	and	and	CC
cord-010188-884d196k	62	23	release	release	NN
cord-010188-884d196k	62	24	of	of	IN
cord-010188-884d196k	62	25	infectious	infectious	JJ
cord-010188-884d196k	62	26	virus	virus	NN
cord-010188-884d196k	62	27	from	from	IN
cord-010188-884d196k	62	28	the	the	DT
cord-010188-884d196k	62	29	host	host	NN
cord-010188-884d196k	62	30	-	-	HYPH
cord-010188-884d196k	62	31	cell	cell	NN
cord-010188-884d196k	62	32	membrane	membrane	NN
cord-010188-884d196k	62	33	.	.	.
cord-010188-884d196k	63	1	The	the	DT
cord-010188-884d196k	63	2	basic	basic	JJ
cord-010188-884d196k	63	3	strategy	strategy	NN
cord-010188-884d196k	63	4	for	for	IN
cord-010188-884d196k	63	5	using	use	VBG
cord-010188-884d196k	63	6	alphaviruses	alphavirus	NNS
cord-010188-884d196k	63	7	as	as	IN
cord-010188-884d196k	63	8	vectors	vector	NNS
cord-010188-884d196k	63	9	for	for	IN
cord-010188-884d196k	63	10	the	the	DT
cord-010188-884d196k	63	11	expression	expression	NN
cord-010188-884d196k	63	12	of	of	IN
cord-010188-884d196k	63	13	heterologous	heterologous	JJ
cord-010188-884d196k	63	14	genes	gene	NNS
cord-010188-884d196k	63	15	has	have	VBZ
cord-010188-884d196k	63	16	been	be	VBN
cord-010188-884d196k	63	17	to	to	TO
cord-010188-884d196k	63	18	construct	construct	VB
cord-010188-884d196k	63	19	cDNAs	cdna	NNS
cord-010188-884d196k	63	20	of	of	IN
cord-010188-884d196k	63	21	the	the	DT
cord-010188-884d196k	63	22	alphavirus	alphavirus	NNP
cord-010188-884d196k	63	23	genome	genome	NNP
cord-010188-884d196k	63	24	,	,	,
cord-010188-884d196k	63	25	in	in	IN
cord-010188-884d196k	63	26	which	which	WDT
cord-010188-884d196k	63	27	the	the	DT
cord-010188-884d196k	63	28	heterologous	heterologous	JJ
cord-010188-884d196k	63	29	gene	gene	NN
cord-010188-884d196k	63	30	is	be	VBZ
cord-010188-884d196k	63	31	placed	place	VBN
cord-010188-884d196k	63	32	downstream	downstream	RB
cord-010188-884d196k	63	33	from	from	IN
cord-010188-884d196k	63	34	the	the	DT
cord-010188-884d196k	63	35	promoter	promoter	NN
cord-010188-884d196k	63	36	used	use	VBD
cord-010188-884d196k	63	37	to	to	TO
cord-010188-884d196k	63	38	transcribe	transcribe	VB
cord-010188-884d196k	63	39	a	a	DT
cord-010188-884d196k	63	40	subgenomic	subgenomic	NNP
cord-010188-884d196k	63	41	RNA	RNA	NNP
cord-010188-884d196k	63	42	13	13	CD
cord-010188-884d196k	63	43	(	(	-LRB-
cord-010188-884d196k	63	44	Fig	Fig	NNP
cord-010188-884d196k	63	45	.	.	.
cord-010188-884d196k	63	46	2a	2a	CD
cord-010188-884d196k	63	47	)	)	-RRB-
cord-010188-884d196k	63	48	.	.	.
cord-010188-884d196k	64	1	The	the	DT
cord-010188-884d196k	64	2	cDNA	cdna	NN
cord-010188-884d196k	64	3	is	be	VBZ
cord-010188-884d196k	64	4	transcribed	transcribe	VBN
cord-010188-884d196k	64	5	in	in	IN
cord-010188-884d196k	64	6	vitro	vitro	FW
cord-010188-884d196k	64	7	using	use	VBG
cord-010188-884d196k	64	8	the	the	DT
cord-010188-884d196k	64	9	SP6	SP6	NNP
cord-010188-884d196k	64	10	I)NA	I)NA	NNP
cord-010188-884d196k	64	11	-	-	HYPH
cord-010188-884d196k	64	12	dependent	dependent	JJ
cord-010188-884d196k	64	13	RNA	RNA	NNP
cord-010188-884d196k	64	14	polymerase	polymerase	NN
cord-010188-884d196k	64	15	and	and	CC
cord-010188-884d196k	64	16	the	the	DT
cord-010188-884d196k	64	17	RNA	RNA	NNP
cord-010188-884d196k	64	18	transcript	transcript	NN
cord-010188-884d196k	64	19	is	be	VBZ
cord-010188-884d196k	64	20	transfected	transfecte	VBN
cord-010188-884d196k	64	21	into	into	IN
cord-010188-884d196k	64	22	cells	cell	NNS
cord-010188-884d196k	64	23	either	either	CC
cord-010188-884d196k	64	24	by	by	IN
cord-010188-884d196k	64	25	lipofection	lipofection	NN
cord-010188-884d196k	64	26	~4	~4	NFP
cord-010188-884d196k	64	27	or	or	CC
cord-010188-884d196k	64	28	electroporation	electroporation	NN
cord-010188-884d196k	65	1	Is	be	VBZ
cord-010188-884d196k	65	2	-methods	-methods	.
cord-010188-884d196k	66	1	that	that	WDT
cord-010188-884d196k	66	2	enhance	enhance	VBP
cord-010188-884d196k	66	3	the	the	DT
cord-010188-884d196k	66	4	uptake	uptake	NN
cord-010188-884d196k	66	5	of	of	IN
cord-010188-884d196k	66	6	nucleic	nucleic	JJ
cord-010188-884d196k	66	7	acids	acid	NNS
cord-010188-884d196k	66	8	into	into	IN
cord-010188-884d196k	66	9	cells	cell	NNS
cord-010188-884d196k	66	10	.	.	.
cord-010188-884d196k	67	1	The	the	DT
cord-010188-884d196k	67	2	genomic	genomic	JJ
cord-010188-884d196k	67	3	RNA	RNA	NNP
cord-010188-884d196k	67	4	contains	contain	VBZ
cord-010188-884d196k	67	5	the	the	DT
cord-010188-884d196k	67	6	genes	gene	NNS
cord-010188-884d196k	67	7	for	for	IN
cord-010188-884d196k	67	8	the	the	DT
cord-010188-884d196k	67	9	nonstructural	nonstructural	JJ
cord-010188-884d196k	67	10	proteins	protein	NNS
cord-010188-884d196k	67	11	and	and	CC
cord-010188-884d196k	67	12	the	the	DT
cord-010188-884d196k	67	13	cis	cis	NNP
cord-010188-884d196k	67	14	-	-	HYPH
cord-010188-884d196k	67	15	actiug	actiug	NNP
cord-010188-884d196k	67	16	sequences	sequence	NNS
cord-010188-884d196k	67	17	important	important	JJ
cord-010188-884d196k	67	18	in	in	IN
cord-010188-884d196k	67	19	the	the	DT
cord-010188-884d196k	67	20	recognition	recognition	NN
cord-010188-884d196k	67	21	of	of	IN
cord-010188-884d196k	67	22	the	the	DT
cord-010188-884d196k	67	23	RNA	RNA	NNP
cord-010188-884d196k	67	24	for	for	IN
cord-010188-884d196k	67	25	replication	replication	NN
cord-010188-884d196k	67	26	and	and	CC
cord-010188-884d196k	67	27	encapsidation	encapsidation	NN
cord-010188-884d196k	67	28	8,9	8,9	CD
cord-010188-884d196k	67	29	.	.	.
cord-010188-884d196k	68	1	The	the	DT
cord-010188-884d196k	68	2	subgenomic	subgenomic	NNP
cord-010188-884d196k	68	3	RNA	RNA	NNP
cord-010188-884d196k	68	4	synthesized	synthesize	VBN
cord-010188-884d196k	68	5	in	in	IN
cord-010188-884d196k	68	6	the	the	DT
cord-010188-884d196k	68	7	transfected	transfecte	VBN
cord-010188-884d196k	68	8	cells	cell	NNS
cord-010188-884d196k	68	9	is	be	VBZ
cord-010188-884d196k	68	10	translated	translate	VBN
cord-010188-884d196k	68	11	into	into	IN
cord-010188-884d196k	68	12	the	the	DT
cord-010188-884d196k	68	13	heterologous	heterologous	JJ
cord-010188-884d196k	68	14	protein	protein	NN
cord-010188-884d196k	68	15	.	.	.
cord-010188-884d196k	69	1	The	the	DT
cord-010188-884d196k	69	2	first	first	JJ
cord-010188-884d196k	69	3	alphavirus	alphavirus	NN
cord-010188-884d196k	69	4	vector	vector	NN
cord-010188-884d196k	69	5	designed	design	VBN
cord-010188-884d196k	69	6	using	use	VBG
cord-010188-884d196k	69	7	this	this	DT
cord-010188-884d196k	69	8	strategy	strategy	NN
cord-010188-884d196k	69	9	replaced	replace	VBD
cord-010188-884d196k	69	10	the	the	DT
cord-010188-884d196k	69	11	viral	viral	JJ
cord-010188-884d196k	69	12	structural	structural	JJ
cord-010188-884d196k	69	13	-	-	HYPH
cord-010188-884d196k	69	14	protein	protein	NN
cord-010188-884d196k	69	15	gencs	genc	NNS
cord-010188-884d196k	69	16	with	with	IN
cord-010188-884d196k	69	17	the	the	DT
cord-010188-884d196k	69	18	gene	gene	NN
cord-010188-884d196k	69	19	for	for	IN
cord-010188-884d196k	69	20	the	the	DT
cord-010188-884d196k	69	21	bacterial	bacterial	JJ
cord-010188-884d196k	69	22	enzyme	enzyme	NN
cord-010188-884d196k	69	23	chloramphenicol	chloramphenicol	NN
cord-010188-884d196k	69	24	acetyltransferase	acetyltransferase	NNP
cord-010188-884d196k	69	25	(	(	-LRB-
cord-010188-884d196k	69	26	CAT	CAT	NNP
cord-010188-884d196k	69	27	)	)	-RRB-
cord-010188-884d196k	69	28	13	13	CD
cord-010188-884d196k	69	29	.	.	.
cord-010188-884d196k	70	1	About	about	RB
cord-010188-884d196k	70	2	10	10	CD
cord-010188-884d196k	70	3	8	8	CD
cord-010188-884d196k	70	4	CAT	cat	NN
cord-010188-884d196k	70	5	polypeptides	polypeptide	NNS
cord-010188-884d196k	70	6	were	be	VBD
cord-010188-884d196k	70	7	produced	produce	VBN
cord-010188-884d196k	70	8	per	per	IN
cord-010188-884d196k	70	9	transfected	transfected	JJ
cord-010188-884d196k	70	10	cell	cell	NN
cord-010188-884d196k	70	11	,	,	,
cord-010188-884d196k	70	12	corresponding	correspond	VBG
cord-010188-884d196k	70	13	to	to	IN
cord-010188-884d196k	70	14	3	3	CD
cord-010188-884d196k	70	15	%	%	NN
cord-010188-884d196k	70	16	of	of	IN
cord-010188-884d196k	70	17	the	the	DT
cord-010188-884d196k	70	18	total	total	JJ
cord-010188-884d196k	70	19	cell	cell	NN
cord-010188-884d196k	70	20	protein	protein	NN
cord-010188-884d196k	70	21	.	.	.
cord-010188-884d196k	71	1	More	more	RBR
cord-010188-884d196k	71	2	recently	recently	RB
cord-010188-884d196k	71	3	,	,	,
cord-010188-884d196k	71	4	a	a	DT
cord-010188-884d196k	71	5	similar	similar	JJ
cord-010188-884d196k	71	6	vector	vector	NN
cord-010188-884d196k	71	7	was	be	VBD
cord-010188-884d196k	71	8	designed	design	VBN
cord-010188-884d196k	71	9	using	use	VBG
cord-010188-884d196k	71	10	the	the	DT
cord-010188-884d196k	71	11	cDNA	cdna	NN
cord-010188-884d196k	71	12	of	of	IN
cord-010188-884d196k	71	13	Semliki	Semliki	NNP
cord-010188-884d196k	71	14	Forest	Forest	NNP
cord-010188-884d196k	71	15	virus	virus	NN
cord-010188-884d196k	71	16	1-~.	1-~.	CD
cord-010188-884d196k	71	17	Infection	infection	NN
cord-010188-884d196k	71	18	by	by	IN
cord-010188-884d196k	71	19	a	a	DT
cord-010188-884d196k	71	20	virus	virus	NN
cord-010188-884d196k	71	21	is	be	VBZ
cord-010188-884d196k	71	22	a	a	DT
cord-010188-884d196k	71	23	much	much	RB
cord-010188-884d196k	71	24	more	more	RBR
cord-010188-884d196k	71	25	efficient	efficient	JJ
cord-010188-884d196k	71	26	means	mean	NNS
cord-010188-884d196k	71	27	for	for	IN
cord-010188-884d196k	71	28	introducing	introduce	VBG
cord-010188-884d196k	71	29	its	-PRON-	PRP$
cord-010188-884d196k	71	30	genome	genome	NN
cord-010188-884d196k	71	31	into	into	IN
cord-010188-884d196k	71	32	the	the	DT
cord-010188-884d196k	71	33	cell	cell	NN
cord-010188-884d196k	71	34	than	than	IN
cord-010188-884d196k	71	35	transfection	transfection	NN
cord-010188-884d196k	71	36	,	,	,
cord-010188-884d196k	71	37	and	and	CC
cord-010188-884d196k	71	38	several	several	JJ
cord-010188-884d196k	71	39	different	different	JJ
cord-010188-884d196k	71	40	strategies	strategy	NNS
cord-010188-884d196k	71	41	have	have	VBP
cord-010188-884d196k	71	42	been	be	VBN
cord-010188-884d196k	71	43	designed	design	VBN
cord-010188-884d196k	71	44	to	to	TO
cord-010188-884d196k	71	45	permit	permit	VB
cord-010188-884d196k	71	46	packaging	packaging	NN
cord-010188-884d196k	71	47	of	of	IN
cord-010188-884d196k	71	48	the	the	DT
cord-010188-884d196k	71	49	RNA	RNA	NNP
cord-010188-884d196k	71	50	vector	vector	NN
cord-010188-884d196k	71	51	.	.	.
cord-010188-884d196k	72	1	Hahn	Hahn	NNP
cord-010188-884d196k	72	2	et	et	NNP
cord-010188-884d196k	72	3	al	al	NNP
cord-010188-884d196k	72	4	.	.	.
cord-010188-884d196k	73	1	16	16	CD
cord-010188-884d196k	73	2	engineered	engineer	VBD
cord-010188-884d196k	73	3	the	the	DT
cord-010188-884d196k	73	4	Sindbis	Sindbis	NNP
cord-010188-884d196k	73	5	virus	virus	NN
cord-010188-884d196k	73	6	cDNA	cdna	NN
cord-010188-884d196k	73	7	so	so	IN
cord-010188-884d196k	73	8	that	that	IN
cord-010188-884d196k	73	9	it	-PRON-	PRP
cord-010188-884d196k	73	10	contained	contain	VBD
cord-010188-884d196k	73	11	two	two	CD
cord-010188-884d196k	73	12	promoters	promoter	NNS
cord-010188-884d196k	73	13	for	for	IN
cord-010188-884d196k	73	14	subgenomic	subgenomic	NNP
cord-010188-884d196k	73	15	RNA	RNA	NNP
cord-010188-884d196k	73	16	synthesis	synthesis	NN
cord-010188-884d196k	73	17	;	;	:
cord-010188-884d196k	73	18	one	one	CD
cord-010188-884d196k	73	19	upstream	upstream	RB
cord-010188-884d196k	73	20	of	of	IN
cord-010188-884d196k	73	21	the	the	DT
cord-010188-884d196k	73	22	Sindbis	Sindbis	NNP
cord-010188-884d196k	73	23	virus	virus	NN
cord-010188-884d196k	73	24	genes	gene	NNS
cord-010188-884d196k	73	25	,	,	,
cord-010188-884d196k	73	26	the	the	DT
cord-010188-884d196k	73	27	other	other	JJ
cord-010188-884d196k	73	28	upstream	upstream	NN
cord-010188-884d196k	73	29	of	of	IN
cord-010188-884d196k	73	30	the	the	DT
cord-010188-884d196k	73	31	heterologous	heterologous	JJ
cord-010188-884d196k	73	32	gene	gene	NN
cord-010188-884d196k	73	33	(	(	-LRB-
cord-010188-884d196k	73	34	Fig	Fig	NNP
cord-010188-884d196k	73	35	.	.	.
cord-010188-884d196k	73	36	2b	2b	NNP
cord-010188-884d196k	73	37	)	)	-RRB-
cord-010188-884d196k	73	38	.	.	.
cord-010188-884d196k	74	1	Cells	cell	NNS
cord-010188-884d196k	74	2	transfected	transfecte	VBN
cord-010188-884d196k	74	3	with	with	IN
cord-010188-884d196k	74	4	the	the	DT
cord-010188-884d196k	74	5	recombinant	recombinant	NN
cord-010188-884d196k	74	6	,	,	,
cord-010188-884d196k	74	7	in	in	IN
cord-010188-884d196k	74	8	vitro	vitro	FW
cord-010188-884d196k	74	9	transcribed	transcribe	VBN
cord-010188-884d196k	74	10	RNA	RNA	NNP
cord-010188-884d196k	74	11	produced	produce	VBD
cord-010188-884d196k	74	12	vires	vires	NNP
cord-010188-884d196k	74	13	with	with	IN
cord-010188-884d196k	74	14	yields	yield	NNS
cord-010188-884d196k	74	15	ranging	range	VBG
cord-010188-884d196k	74	16	from	from	IN
cord-010188-884d196k	74	17	10	10	CD
cord-010188-884d196k	74	18	8	8	CD
cord-010188-884d196k	74	19	to	to	TO
cord-010188-884d196k	74	20	10	10	CD
cord-010188-884d196k	74	21	9	9	CD
cord-010188-884d196k	74	22	plaque	plaque	NN
cord-010188-884d196k	74	23	forming	form	VBG
cord-010188-884d196k	74	24	units	unit	NNS
cord-010188-884d196k	74	25	(	(	-LRB-
cord-010188-884d196k	74	26	PFU	pfu	NN
cord-010188-884d196k	74	27	)	)	-RRB-
cord-010188-884d196k	74	28	per	per	IN
cord-010188-884d196k	74	29	ml	ml	NN
cord-010188-884d196k	74	30	.	.	.
cord-010188-884d196k	75	1	The	the	DT
cord-010188-884d196k	75	2	vector	vector	NN
cord-010188-884d196k	75	3	studied	study	VBD
cord-010188-884d196k	75	4	in	in	IN
cord-010188-884d196k	75	5	the	the	DT
cord-010188-884d196k	75	6	greatest	great	JJS
cord-010188-884d196k	75	7	detail	detail	NN
cord-010188-884d196k	75	8	contained	contain	VBD
cord-010188-884d196k	75	9	the	the	DT
cord-010188-884d196k	75	10	heterologous	heterologous	JJ
cord-010188-884d196k	75	11	gene	gene	NN
cord-010188-884d196k	75	12	inserted	insert	VBN
cord-010188-884d196k	75	13	downstream	downstream	RB
cord-010188-884d196k	75	14	from	from	IN
cord-010188-884d196k	75	15	the	the	DT
cord-010188-884d196k	75	16	structural	structural	JJ
cord-010188-884d196k	75	17	genes	gene	NNS
cord-010188-884d196k	75	18	.	.	.
cord-010188-884d196k	76	1	Infection	infection	NN
cord-010188-884d196k	76	2	of	of	IN
cord-010188-884d196k	76	3	cells	cell	NNS
cord-010188-884d196k	76	4	with	with	IN
cord-010188-884d196k	76	5	the	the	DT
cord-010188-884d196k	76	6	double	double	JJ
cord-010188-884d196k	76	7	subgenomic	subgenomic	NNP
cord-010188-884d196k	76	8	RNA	RNA	NNP
cord-010188-884d196k	76	9	Sindbis	Sindbis	NNP
cord-010188-884d196k	76	10	virus	virus	NN
cord-010188-884d196k	76	11	vector	vector	NN
cord-010188-884d196k	76	12	resulted	result	VBD
cord-010188-884d196k	76	13	in	in	IN
cord-010188-884d196k	76	14	the	the	DT
cord-010188-884d196k	76	15	production	production	NN
cord-010188-884d196k	76	16	of	of	IN
cord-010188-884d196k	76	17	>	>	XX
cord-010188-884d196k	76	18	t0	t0	NN
cord-010188-884d196k	76	19	6	6	CD
cord-010188-884d196k	76	20	CAT	cat	NN
cord-010188-884d196k	76	21	molecules	molecule	NNS
cord-010188-884d196k	76	22	seven	seven	CD
cord-010188-884d196k	76	23	hours	hour	NNS
cord-010188-884d196k	76	24	after	after	IN
cord-010188-884d196k	76	25	transfection	transfection	NN
cord-010188-884d196k	76	26	.	.	.
cord-010188-884d196k	77	1	The	the	DT
cord-010188-884d196k	77	2	size	size	NN
cord-010188-884d196k	77	3	of	of	IN
cord-010188-884d196k	77	4	the	the	DT
cord-010188-884d196k	77	5	heterologous	heterologous	JJ
cord-010188-884d196k	77	6	insert	insert	NN
cord-010188-884d196k	77	7	can	can	MD
cord-010188-884d196k	77	8	affect	affect	VB
cord-010188-884d196k	77	9	the	the	DT
cord-010188-884d196k	77	10	stability	stability	NN
cord-010188-884d196k	77	11	of	of	IN
cord-010188-884d196k	77	12	the	the	DT
cord-010188-884d196k	77	13	recombinant	recombinant	JJ
cord-010188-884d196k	77	14	RNA	RNA	NNP
cord-010188-884d196k	77	15	.	.	.
cord-010188-884d196k	78	1	Large	large	JJ
cord-010188-884d196k	78	2	inserts	insert	NNS
cord-010188-884d196k	78	3	(	(	-LRB-
cord-010188-884d196k	78	4	i.e.	i.e.	FW
cord-010188-884d196k	78	5	>	>	XX
cord-010188-884d196k	78	6	3	3	CD
cord-010188-884d196k	78	7	kb	kb	NNP
cord-010188-884d196k	78	8	)	)	-RRB-
cord-010188-884d196k	78	9	are	be	VBP
cord-010188-884d196k	78	10	much	much	RB
cord-010188-884d196k	78	11	less	less	RBR
cord-010188-884d196k	78	12	stable	stable	JJ
cord-010188-884d196k	78	13	than	than	IN
cord-010188-884d196k	78	14	smaller	small	JJR
cord-010188-884d196k	78	15	ones	one	NNS
cord-010188-884d196k	78	16	(	(	-LRB-
cord-010188-884d196k	78	17	i.e.	i.e.	FW
cord-010188-884d196k	78	18	<	<	XX
cord-010188-884d196k	78	19	2	2	CD
cord-010188-884d196k	78	20	kb	kb	NNP
cord-010188-884d196k	78	21	)	)	-RRB-
cord-010188-884d196k	78	22	.	.	.
cord-010188-884d196k	79	1	The	the	DT
cord-010188-884d196k	79	2	level	level	NN
cord-010188-884d196k	79	3	of	of	IN
cord-010188-884d196k	79	4	expression	expression	NN
cord-010188-884d196k	79	5	of	of	IN
cord-010188-884d196k	79	6	the	the	DT
cord-010188-884d196k	79	7	heterologous	heterologous	JJ
cord-010188-884d196k	79	8	gene	gene	NN
cord-010188-884d196k	79	9	is	be	VBZ
cord-010188-884d196k	79	10	higher	high	JJR
cord-010188-884d196k	79	11	when	when	WRB
cord-010188-884d196k	79	12	it	-PRON-	PRP
cord-010188-884d196k	79	13	is	be	VBZ
cord-010188-884d196k	79	14	located	locate	VBN
cord-010188-884d196k	79	15	downstream	downstream	RB
cord-010188-884d196k	79	16	from	from	IN
cord-010188-884d196k	79	17	the	the	DT
cord-010188-884d196k	79	18	structural	structural	JJ
cord-010188-884d196k	79	19	genes	gene	NNS
cord-010188-884d196k	79	20	,	,	,
cord-010188-884d196k	79	21	although	although	IN
cord-010188-884d196k	79	22	the	the	DT
cord-010188-884d196k	79	23	recombinant	recombinant	JJ
cord-010188-884d196k	79	24	RNA	RNA	NNP
cord-010188-884d196k	79	25	appears	appear	VBZ
cord-010188-884d196k	79	26	to	to	TO
cord-010188-884d196k	79	27	be	be	VB
cord-010188-884d196k	79	28	more	more	RBR
cord-010188-884d196k	79	29	stable	stable	JJ
cord-010188-884d196k	79	30	when	when	WRB
cord-010188-884d196k	79	31	placed	place	VBN
cord-010188-884d196k	79	32	upstream	upstream	RB
cord-010188-884d196k	79	33	of	of	IN
cord-010188-884d196k	79	34	the	the	DT
cord-010188-884d196k	79	35	structural	structural	JJ
cord-010188-884d196k	79	36	gene	gene	NN
cord-010188-884d196k	79	37	.	.	.
cord-010188-884d196k	80	1	The	the	DT
cord-010188-884d196k	80	2	alphavirus	alphavirus	NNP
cord-010188-884d196k	80	3	vector	vector	NN
cord-010188-884d196k	80	4	containing	contain	VBG
cord-010188-884d196k	80	5	a	a	DT
cord-010188-884d196k	80	6	single	single	JJ
cord-010188-884d196k	80	7	subgenomic	subgenomic	NNP
cord-010188-884d196k	80	8	RNA	RNA	NNP
cord-010188-884d196k	80	9	promoter	promoter	NN
cord-010188-884d196k	80	10	can	can	MD
cord-010188-884d196k	80	11	be	be	VB
cord-010188-884d196k	80	12	packaged	package	VBN
cord-010188-884d196k	80	13	by	by	IN
cord-010188-884d196k	80	14	complementation	complementation	NN
cord-010188-884d196k	80	15	with	with	IN
cord-010188-884d196k	80	16	a	a	DT
cord-010188-884d196k	80	17	defective	defective	JJ
cord-010188-884d196k	80	18	helper	helper	NN
cord-010188-884d196k	80	19	-	-	HYPH
cord-010188-884d196k	80	20	virus	virus	NN
cord-010188-884d196k	80	21	RNA	RNA	NNP
cord-010188-884d196k	80	22	(	(	-LRB-
cord-010188-884d196k	80	23	Fig	Fig	NNP
cord-010188-884d196k	80	24	.	.	.
cord-010188-884d196k	80	25	 	 	_SP
cord-010188-884d196k	80	26	3	3	CD
cord-010188-884d196k	80	27	)	)	-RRB-
cord-010188-884d196k	80	28	.	.	.
cord-010188-884d196k	81	1	Two	two	CD
cord-010188-884d196k	81	2	types	type	NNS
cord-010188-884d196k	81	3	of	of	IN
cord-010188-884d196k	81	4	packaging	package	VBG
cord-010188-884d196k	81	5	helper	helper	NN
cord-010188-884d196k	81	6	RNAs	rna	NNS
cord-010188-884d196k	81	7	have	have	VBP
cord-010188-884d196k	81	8	been	be	VBN
cord-010188-884d196k	81	9	used	use	VBN
cord-010188-884d196k	81	10	:	:	:
cord-010188-884d196k	81	11	•	•	XX
cord-010188-884d196k	82	1	One	one	CD
cord-010188-884d196k	82	2	,	,	,
cord-010188-884d196k	82	3	first	first	RB
cord-010188-884d196k	82	4	described	describe	VBN
cord-010188-884d196k	82	5	by	by	IN
cord-010188-884d196k	82	6	Geigenmtiller	Geigenmtiller	NNP
cord-010188-884d196k	82	7	-	-	HYPH
cord-010188-884d196k	82	8	Gnirke	Gnirke	NNP
cord-010188-884d196k	82	9	et	et	NNP
cord-010188-884d196k	82	10	al	al	NNP
cord-010188-884d196k	82	11	.	.	.
cord-010188-884d196k	83	1	17	17	CD
cord-010188-884d196k	83	2	,	,	,
cord-010188-884d196k	83	3	contains	contain	VBZ
cord-010188-884d196k	83	4	the	the	DT
cord-010188-884d196k	83	5	structural	structural	JJ
cord-010188-884d196k	83	6	genes	gene	NNS
cord-010188-884d196k	83	7	downstream	downstream	RB
cord-010188-884d196k	83	8	from	from	IN
cord-010188-884d196k	83	9	the	the	DT
cord-010188-884d196k	83	10	promoter	promoter	NN
cord-010188-884d196k	83	11	for	for	IN
cord-010188-884d196k	83	12	the	the	DT
cord-010188-884d196k	83	13	subgenomic	subgenomic	NNP
cord-010188-884d196k	83	14	RNA	RNA	NNP
cord-010188-884d196k	83	15	and	and	CC
cord-010188-884d196k	83	16	also	also	RB
cord-010188-884d196k	83	17	contains	contain	VBZ
cord-010188-884d196k	83	18	the	the	DT
cord-010188-884d196k	83	19	cis	cis	NNP
cord-010188-884d196k	83	20	-	-	HYPH
cord-010188-884d196k	83	21	acting	acting	NN
cord-010188-884d196k	83	22	sequences	sequence	NNS
cord-010188-884d196k	83	23	required	require	VBN
cord-010188-884d196k	83	24	for	for	IN
cord-010188-884d196k	83	25	replication	replication	NN
cord-010188-884d196k	83	26	and	and	CC
cord-010188-884d196k	83	27	encapsidation	encapsidation	NN
cord-010188-884d196k	83	28	(	(	-LRB-
cord-010188-884d196k	83	29	Fig	Fig	NNP
cord-010188-884d196k	83	30	.	.	.
cord-010188-884d196k	83	31	3	3	CD
cord-010188-884d196k	83	32	)	)	-RRB-
cord-010188-884d196k	83	33	.	.	.
cord-010188-884d196k	84	1	Most	Most	JJS
cord-010188-884d196k	84	2	of	of	IN
cord-010188-884d196k	84	3	the	the	DT
cord-010188-884d196k	84	4	released	release	VBN
cord-010188-884d196k	84	5	particles	particle	NNS
cord-010188-884d196k	84	6	contain	contain	VBP
cord-010188-884d196k	84	7	both	both	CC
cord-010188-884d196k	84	8	the	the	DT
cord-010188-884d196k	84	9	helper	helper	NN
cord-010188-884d196k	84	10	and	and	CC
cord-010188-884d196k	84	11	vector	vector	NN
cord-010188-884d196k	84	12	RNAs	rna	NNS
cord-010188-884d196k	84	13	in	in	IN
cord-010188-884d196k	84	14	a	a	DT
cord-010188-884d196k	84	15	single	single	JJ
cord-010188-884d196k	84	16	particle	particle	NN
cord-010188-884d196k	84	17	;	;	:
cord-010188-884d196k	84	18	such	such	JJ
cord-010188-884d196k	84	19	particles	particle	NNS
cord-010188-884d196k	84	20	are	be	VBP
cord-010188-884d196k	84	21	able	able	JJ
cord-010188-884d196k	84	22	to	to	TO
cord-010188-884d196k	84	23	form	form	VB
cord-010188-884d196k	84	24	plaques	plaque	NNS
cord-010188-884d196k	84	25	and	and	CC
cord-010188-884d196k	84	26	appear	appear	VBP
cord-010188-884d196k	84	27	to	to	TO
cord-010188-884d196k	84	28	be	be	VB
cord-010188-884d196k	84	29	stable	stable	JJ
cord-010188-884d196k	84	30	over	over	IN
cord-010188-884d196k	84	31	several	several	JJ
cord-010188-884d196k	84	32	passages	passage	NNS
cord-010188-884d196k	84	33	(	(	-LRB-
cord-010188-884d196k	84	34	i.e.	i.e.	FW
cord-010188-884d196k	84	35	repeated	repeat	VBN
cord-010188-884d196k	84	36	subculture	subculture	NN
cord-010188-884d196k	84	37	)	)	-RRB-
cord-010188-884d196k	84	38	.	.	.
cord-010188-884d196k	85	1	This	this	DT
cord-010188-884d196k	85	2	type	type	NN
cord-010188-884d196k	85	3	of	of	IN
cord-010188-884d196k	85	4	packaged	package	VBN
cord-010188-884d196k	85	5	vector	vector	NN
cord-010188-884d196k	85	6	should	should	MD
cord-010188-884d196k	85	7	prove	prove	VB
cord-010188-884d196k	85	8	to	to	TO
cord-010188-884d196k	85	9	be	be	VB
cord-010188-884d196k	85	10	of	of	IN
cord-010188-884d196k	85	11	value	value	NN
cord-010188-884d196k	85	12	in	in	IN
cord-010188-884d196k	85	13	animal	animal	NN
cord-010188-884d196k	85	14	experiments	experiment	NNS
cord-010188-884d196k	85	15	in	in	IN
cord-010188-884d196k	85	16	which	which	WDT
cord-010188-884d196k	85	17	both	both	DT
cord-010188-884d196k	85	18	production	production	NN
cord-010188-884d196k	85	19	of	of	IN
cord-010188-884d196k	85	20	the	the	DT
cord-010188-884d196k	85	21	heterologous	heterologous	JJ
cord-010188-884d196k	85	22	gene	gene	NN
cord-010188-884d196k	85	23	and	and	CC
cord-010188-884d196k	85	24	spread	spread	NN
cord-010188-884d196k	85	25	of	of	IN
cord-010188-884d196k	85	26	the	the	DT
cord-010188-884d196k	85	27	virus	virus	NN
cord-010188-884d196k	85	28	is	be	VBZ
cord-010188-884d196k	85	29	desired	desire	VBN
cord-010188-884d196k	85	30	.	.	.
cord-010188-884d196k	86	1	revieues	revieues	NNP
cord-010188-884d196k	87	1	•	•	NNP
cord-010188-884d196k	88	1	The	the	DT
cord-010188-884d196k	88	2	second	second	JJ
cord-010188-884d196k	88	3	type	type	NN
cord-010188-884d196k	88	4	of	of	IN
cord-010188-884d196k	88	5	defective	defective	JJ
cord-010188-884d196k	88	6	helper	helper	NN
cord-010188-884d196k	88	7	-	-	HYPH
cord-010188-884d196k	88	8	virus	virus	NN
cord-010188-884d196k	88	9	RNA	RNA	NNP
cord-010188-884d196k	88	10	lacks	lack	VBZ
cord-010188-884d196k	88	11	the	the	DT
cord-010188-884d196k	88	12	region	region	NN
cord-010188-884d196k	88	13	required	require	VBN
cord-010188-884d196k	88	14	for	for	IN
cord-010188-884d196k	88	15	encapsidation	encapsidation	NN
cord-010188-884d196k	88	16	of	of	IN
cord-010188-884d196k	88	17	the	the	DT
cord-010188-884d196k	88	18	RNA	RNA	NNP
cord-010188-884d196k	88	19	.	.	.
cord-010188-884d196k	89	1	In	in	IN
cord-010188-884d196k	89	2	studies	study	NNS
cord-010188-884d196k	89	3	of	of	IN
cord-010188-884d196k	89	4	the	the	DT
cord-010188-884d196k	89	5	packaging	packaging	NN
cord-010188-884d196k	89	6	of	of	IN
cord-010188-884d196k	89	7	the	the	DT
cord-010188-884d196k	89	8	Semliki	Semliki	NNP
cord-010188-884d196k	89	9	Forest	Forest	NNP
cord-010188-884d196k	89	10	virus	virus	NN
cord-010188-884d196k	89	11	vector	vector	NN
cord-010188-884d196k	89	12	,	,	,
cord-010188-884d196k	89	13	Liljestr6	Liljestr6	NNP
cord-010188-884d196k	89	14	m	m	NNP
cord-010188-884d196k	89	15	and	and	CC
cord-010188-884d196k	89	16	Garoff	Garoff	NNP
cord-010188-884d196k	89	17	Ls	Ls	NNP
cord-010188-884d196k	89	18	found	find	VBD
cord-010188-884d196k	89	19	that	that	IN
cord-010188-884d196k	89	20	cotransfection	cotransfection	NN
cord-010188-884d196k	89	21	of	of	IN
cord-010188-884d196k	89	22	the	the	DT
cord-010188-884d196k	89	23	latter	latter	JJ
cord-010188-884d196k	89	24	type	type	NN
cord-010188-884d196k	89	25	of	of	IN
cord-010188-884d196k	89	26	helper	helper	NN
cord-010188-884d196k	89	27	RNA	RNA	NNP
cord-010188-884d196k	89	28	with	with	IN
cord-010188-884d196k	89	29	vector	vector	NNP
cord-010188-884d196k	89	30	RNA	RNA	NNP
cord-010188-884d196k	89	31	led	lead	VBD
cord-010188-884d196k	89	32	to	to	IN
cord-010188-884d196k	89	33	efficient	efficient	JJ
cord-010188-884d196k	89	34	packaging	packaging	NN
cord-010188-884d196k	89	35	of	of	IN
cord-010188-884d196k	89	36	the	the	DT
cord-010188-884d196k	89	37	vector	vector	NN
cord-010188-884d196k	89	38	RNA	RNA	NNP
cord-010188-884d196k	89	39	,	,	,
cord-010188-884d196k	89	40	but	but	CC
cord-010188-884d196k	89	41	the	the	DT
cord-010188-884d196k	89	42	helper	helper	NN
cord-010188-884d196k	89	43	RNA	RNA	NNP
cord-010188-884d196k	89	44	was	be	VBD
cord-010188-884d196k	89	45	not	not	RB
cord-010188-884d196k	89	46	packaged	package	VBN
cord-010188-884d196k	89	47	.	.	.
cord-010188-884d196k	90	1	Electroporation	electroporation	NN
cord-010188-884d196k	90	2	of	of	IN
cord-010188-884d196k	90	3	both	both	DT
cord-010188-884d196k	90	4	helper	helper	NN
cord-010188-884d196k	90	5	and	and	CC
cord-010188-884d196k	90	6	vector	vector	NN
cord-010188-884d196k	90	7	RNAs	rna	NNS
cord-010188-884d196k	90	8	produced	produce	VBD
cord-010188-884d196k	90	9	yields	yield	NNS
cord-010188-884d196k	90	10	of	of	IN
cord-010188-884d196k	90	11	up	up	IN
cord-010188-884d196k	90	12	to	to	TO
cord-010188-884d196k	90	13	10	10	CD
cord-010188-884d196k	90	14	9	9	CD
cord-010188-884d196k	90	15	particles	particle	NNS
cord-010188-884d196k	90	16	from	from	IN
cord-010188-884d196k	90	17	5	5	CD
cord-010188-884d196k	90	18	x	x	SYM
cord-010188-884d196k	90	19	10	10	CD
cord-010188-884d196k	90	20	6	6	CD
cord-010188-884d196k	90	21	cells	cell	NNS
cord-010188-884d196k	90	22	(	(	-LRB-
cord-010188-884d196k	90	23	as	as	IN
cord-010188-884d196k	90	24	observed	observe	VBN
cord-010188-884d196k	90	25	by	by	IN
cord-010188-884d196k	90	26	immunofluorescence	immunofluorescence	NN
cord-010188-884d196k	90	27	analysis	analysis	NN
cord-010188-884d196k	90	28	)	)	-RRB-
cord-010188-884d196k	90	29	.	.	.
cord-010188-884d196k	91	1	Packaging	packaging	NN
cord-010188-884d196k	91	2	of	of	IN
cord-010188-884d196k	91	3	the	the	DT
cord-010188-884d196k	91	4	Sindbis	Sindbis	NNP
cord-010188-884d196k	91	5	virus	virus	NN
cord-010188-884d196k	91	6	vector	vector	NN
cord-010188-884d196k	91	7	using	use	VBG
cord-010188-884d196k	91	8	Sindbis	Sindbis	NNP
cord-010188-884d196k	91	9	defective	defective	JJ
cord-010188-884d196k	91	10	helper	helper	NN
cord-010188-884d196k	91	11	RNAs	rna	NNS
cord-010188-884d196k	91	12	has	have	VBZ
cord-010188-884d196k	91	13	proved	prove	VBN
cord-010188-884d196k	91	14	to	to	TO
cord-010188-884d196k	91	15	be	be	VB
cord-010188-884d196k	91	16	more	more	RBR
cord-010188-884d196k	91	17	complex	complex	JJ
cord-010188-884d196k	91	18	(	(	-LRB-
cord-010188-884d196k	91	19	P.	P.	NNP
cord-010188-884d196k	91	20	Bredenbeck	Bredenbeck	NNP
cord-010188-884d196k	91	21	,	,	,
cord-010188-884d196k	91	22	C.	C.	NNP
cord-010188-884d196k	91	23	Rice	Rice	NNP
cord-010188-884d196k	91	24	,	,	,
cord-010188-884d196k	91	25	I.	I.	NNP
cord-010188-884d196k	91	26	Frolov	Frolov	NNP
cord-010188-884d196k	91	27	and	and	CC
cord-010188-884d196k	91	28	S.	S.	NNP
cord-010188-884d196k	91	29	Schlcsinger	Schlcsinger	NNP
cord-010188-884d196k	91	30	,	,	,
cord-010188-884d196k	91	31	unpublished	unpublished	JJ
cord-010188-884d196k	91	32	)	)	-RRB-
cord-010188-884d196k	91	33	.	.	.
cord-010188-884d196k	92	1	Deletion	deletion	NN
cord-010188-884d196k	92	2	of	of	IN
cord-010188-884d196k	92	3	the	the	DT
cord-010188-884d196k	92	4	packaging	packaging	NN
cord-010188-884d196k	92	5	signal	signal	NN
cord-010188-884d196k	92	6	leads	lead	VBZ
cord-010188-884d196k	92	7	to	to	IN
cord-010188-884d196k	92	8	a	a	DT
cord-010188-884d196k	92	9	considerable	considerable	JJ
cord-010188-884d196k	92	10	reduction	reduction	NN
cord-010188-884d196k	92	11	in	in	IN
cord-010188-884d196k	92	12	the	the	DT
cord-010188-884d196k	92	13	amount	amount	NN
cord-010188-884d196k	92	14	of	of	IN
cord-010188-884d196k	92	15	helper	helper	NN
cord-010188-884d196k	92	16	RNA	RNA	NNP
cord-010188-884d196k	92	17	in	in	IN
cord-010188-884d196k	92	18	the	the	DT
cord-010188-884d196k	92	19	progeny	progeny	NN
cord-010188-884d196k	92	20	;	;	:
cord-010188-884d196k	92	21	however	however	RB
cord-010188-884d196k	92	22	,	,	,
cord-010188-884d196k	92	23	its	-PRON-	PRP$
cord-010188-884d196k	92	24	presence	presence	NN
cord-010188-884d196k	92	25	can	can	MD
cord-010188-884d196k	92	26	still	still	RB
cord-010188-884d196k	92	27	be	be	VB
cord-010188-884d196k	92	28	detected	detect	VBN
cord-010188-884d196k	92	29	by	by	IN
cord-010188-884d196k	92	30	analysis	analysis	NN
cord-010188-884d196k	92	31	of	of	IN
cord-010188-884d196k	92	32	the	the	DT
cord-010188-884d196k	92	33	RNA	RNA	NNP
cord-010188-884d196k	92	34	synthesized	synthesize	VBN
cord-010188-884d196k	92	35	in	in	IN
cord-010188-884d196k	92	36	vector	vector	NN
cord-010188-884d196k	92	37	-	-	HYPH
cord-010188-884d196k	92	38	infected	infect	VBN
cord-010188-884d196k	92	39	cells	cell	NNS
cord-010188-884d196k	92	40	,	,	,
cord-010188-884d196k	92	41	and	and	CC
cord-010188-884d196k	92	42	by	by	IN
cord-010188-884d196k	92	43	a	a	DT
cord-010188-884d196k	92	44	low	low	JJ
cord-010188-884d196k	92	45	level	level	NN
cord-010188-884d196k	92	46	of	of	IN
cord-010188-884d196k	92	47	PFU	pfu	NN
cord-010188-884d196k	92	48	in	in	IN
cord-010188-884d196k	92	49	the	the	DT
cord-010188-884d196k	92	50	media	medium	NNS
cord-010188-884d196k	92	51	,	,	,
cord-010188-884d196k	92	52	presumably	presumably	RB
cord-010188-884d196k	92	53	due	due	IN
cord-010188-884d196k	92	54	to	to	IN
cord-010188-884d196k	92	55	copackaging	copackage	VBG
cord-010188-884d196k	92	56	of	of	IN
cord-010188-884d196k	92	57	the	the	DT
cord-010188-884d196k	92	58	RNAs	rna	NNS
cord-010188-884d196k	92	59	.	.	.
cord-010188-884d196k	93	1	This	this	DT
cord-010188-884d196k	93	2	may	may	MD
cord-010188-884d196k	93	3	be	be	VB
cord-010188-884d196k	93	4	due	due	JJ
cord-010188-884d196k	93	5	to	to	IN
cord-010188-884d196k	93	6	the	the	DT
cord-010188-884d196k	93	7	ability	ability	NN
cord-010188-884d196k	93	8	of	of	IN
cord-010188-884d196k	93	9	the	the	DT
cord-010188-884d196k	93	10	encapsidation	encapsidation	NN
cord-010188-884d196k	93	11	process	process	NN
cord-010188-884d196k	93	12	to	to	TO
cord-010188-884d196k	93	13	include	include	VB
cord-010188-884d196k	93	14	other	other	JJ
cord-010188-884d196k	93	15	RNAs	rna	NNS
cord-010188-884d196k	93	16	in	in	IN
cord-010188-884d196k	93	17	the	the	DT
cord-010188-884d196k	93	18	capsid	capsid	NN
cord-010188-884d196k	93	19	in	in	IN
cord-010188-884d196k	93	20	conjunction	conjunction	NN
cord-010188-884d196k	93	21	with	with	IN
cord-010188-884d196k	93	22	the	the	DT
cord-010188-884d196k	93	23	viral	viral	JJ
cord-010188-884d196k	93	24	RNA	RNA	NNP
cord-010188-884d196k	93	25	encoding	encode	VBG
cord-010188-884d196k	93	26	the	the	DT
cord-010188-884d196k	93	27	specific	specific	JJ
cord-010188-884d196k	93	28	packaging	packaging	NN
cord-010188-884d196k	93	29	signal	signal	NN
cord-010188-884d196k	93	30	.	.	.
cord-010188-884d196k	94	1	A	a	DT
cord-010188-884d196k	94	2	second	second	JJ
cord-010188-884d196k	94	3	potential	potential	JJ
cord-010188-884d196k	94	4	problem	problem	NN
cord-010188-884d196k	94	5	is	be	VBZ
cord-010188-884d196k	94	6	recombination	recombination	NN
cord-010188-884d196k	94	7	between	between	IN
cord-010188-884d196k	94	8	the	the	DT
cord-010188-884d196k	94	9	packaging	packaging	NN
cord-010188-884d196k	94	10	helper	helper	NN
cord-010188-884d196k	94	11	virus	virus	NN
cord-010188-884d196k	94	12	RNA	rna	NN
cord-010188-884d196k	94	13	and	and	CC
cord-010188-884d196k	94	14	vector	vector	NN
cord-010188-884d196k	94	15	RNAs	rna	NNS
cord-010188-884d196k	94	16	.	.	.
cord-010188-884d196k	95	1	The	the	DT
cord-010188-884d196k	95	2	two	two	CD
cord-010188-884d196k	95	3	Sindbis	Sindbis	NNP
cord-010188-884d196k	95	4	RNAs	rna	NNS
cord-010188-884d196k	95	5	can	can	MD
cord-010188-884d196k	95	6	undergo	undergo	VB
cord-010188-884d196k	95	7	recombination	recombination	NN
cord-010188-884d196k	95	8	to	to	TO
cord-010188-884d196k	95	9	produce	produce	VB
cord-010188-884d196k	95	10	a	a	DT
cord-010188-884d196k	95	11	single	single	JJ
cord-010188-884d196k	95	12	molecule	molecule	NN
cord-010188-884d196k	95	13	of	of	IN
cord-010188-884d196k	95	14	RNA	RNA	NNP
cord-010188-884d196k	95	15	containing	contain	VBG
cord-010188-884d196k	95	16	the	the	DT
cord-010188-884d196k	95	17	genes	gene	NNS
cord-010188-884d196k	95	18	that	that	WDT
cord-010188-884d196k	95	19	encode	encode	VBP
cord-010188-884d196k	95	20	both	both	CC
cord-010188-884d196k	95	21	the	the	DT
cord-010188-884d196k	95	22	nonstructural	nonstructural	JJ
cord-010188-884d196k	95	23	and	and	CC
cord-010188-884d196k	95	24	structural	structural	JJ
cord-010188-884d196k	95	25	proteins	protein	NNS
cord-010188-884d196k	95	26	m.	m.	NN
cord-010188-884d196k	96	1	Although	although	IN
cord-010188-884d196k	96	2	the	the	DT
cord-010188-884d196k	96	3	growth	growth	NN
cord-010188-884d196k	96	4	and	and	CC
cord-010188-884d196k	96	5	replication	replication	NN
cord-010188-884d196k	96	6	of	of	IN
cord-010188-884d196k	96	7	such	such	JJ
cord-010188-884d196k	96	8	recombinants	recombinant	NNS
cord-010188-884d196k	96	9	are	be	VBP
cord-010188-884d196k	96	10	suppressed	suppress	VBN
cord-010188-884d196k	96	11	under	under	IN
cord-010188-884d196k	96	12	conditions	condition	NNS
cord-010188-884d196k	96	13	in	in	IN
cord-010188-884d196k	96	14	which	which	WDT
cord-010188-884d196k	96	15	most	most	JJS
cord-010188-884d196k	96	16	of	of	IN
cord-010188-884d196k	96	17	the	the	DT
cord-010188-884d196k	96	18	cells	cell	NNS
cord-010188-884d196k	96	19	have	have	VBP
cord-010188-884d196k	96	20	been	be	VBN
cord-010188-884d196k	96	21	transfected	transfecte	VBN
cord-010188-884d196k	96	22	with	with	IN
cord-010188-884d196k	96	23	both	both	DT
cord-010188-884d196k	96	24	helper	helper	NN
cord-010188-884d196k	96	25	and	and	CC
cord-010188-884d196k	96	26	vector	vector	NN
cord-010188-884d196k	96	27	RNAs	rna	NNS
cord-010188-884d196k	96	28	,	,	,
cord-010188-884d196k	96	29	they	-PRON-	PRP
cord-010188-884d196k	96	30	can	can	MD
cord-010188-884d196k	96	31	be	be	VB
cord-010188-884d196k	96	32	detected	detect	VBN
cord-010188-884d196k	96	33	after	after	IN
cord-010188-884d196k	96	34	passaging	passage	VBG
cord-010188-884d196k	96	35	of	of	IN
cord-010188-884d196k	96	36	the	the	DT
cord-010188-884d196k	96	37	infectious	infectious	JJ
cord-010188-884d196k	96	38	particles	particle	NNS
cord-010188-884d196k	96	39	.	.	.
cord-010188-884d196k	97	1	A	a	DT
cord-010188-884d196k	97	2	completely	completely	RB
cord-010188-884d196k	97	3	different	different	JJ
cord-010188-884d196k	97	4	approach	approach	NN
cord-010188-884d196k	97	5	to	to	IN
cord-010188-884d196k	97	6	using	use	VBG
cord-010188-884d196k	97	7	alphaviruses	alphaviruses	NNP
cord-010188-884d196k	97	8	as	as	IN
cord-010188-884d196k	97	9	vectors	vector	NNS
cord-010188-884d196k	97	10	has	have	VBZ
cord-010188-884d196k	97	11	been	be	VBN
cord-010188-884d196k	97	12	to	to	TO
cord-010188-884d196k	97	13	insert	insert	VB
cord-010188-884d196k	97	14	heterologous	heterologous	JJ
cord-010188-884d196k	97	15	sequences	sequence	NNS
cord-010188-884d196k	97	16	into	into	IN
cord-010188-884d196k	97	17	the	the	DT
cord-010188-884d196k	97	18	viral	viral	JJ
cord-010188-884d196k	97	19	structural	structural	JJ
cord-010188-884d196k	97	20	proteins	protein	NNS
cord-010188-884d196k	97	21	in	in	IN
cord-010188-884d196k	97	22	such	such	PDT
cord-010188-884d196k	97	23	a	a	DT
cord-010188-884d196k	97	24	way	way	NN
cord-010188-884d196k	97	25	that	that	WDT
cord-010188-884d196k	97	26	the	the	DT
cord-010188-884d196k	97	27	virus	virus	NN
cord-010188-884d196k	97	28	is	be	VBZ
cord-010188-884d196k	97	29	still	still	RB
cord-010188-884d196k	97	30	viable	viable	JJ
cord-010188-884d196k	97	31	,	,	,
cord-010188-884d196k	97	32	but	but	CC
cord-010188-884d196k	97	33	the	the	DT
cord-010188-884d196k	97	34	heterologous	heterologous	JJ
cord-010188-884d196k	97	35	epitope	epitope	NNP
cord-010188-884d196k	97	36	is	be	VBZ
cord-010188-884d196k	97	37	expressed	express	VBN
cord-010188-884d196k	97	38	and	and	CC
cord-010188-884d196k	97	39	can	can	MD
cord-010188-884d196k	97	40	function	function	VB
cord-010188-884d196k	97	41	as	as	IN
cord-010188-884d196k	97	42	an	an	DT
cord-010188-884d196k	97	43	immunogen	immunogen	NN
cord-010188-884d196k	97	44	~9	~9	XX
cord-010188-884d196k	97	45	.	.	.
cord-010188-884d196k	98	1	This	this	DT
cord-010188-884d196k	98	2	concept	concept	NN
cord-010188-884d196k	98	3	was	be	VBD
cord-010188-884d196k	98	4	originally	originally	RB
cord-010188-884d196k	98	5	tested	test	VBN
cord-010188-884d196k	98	6	with	with	IN
cord-010188-884d196k	98	7	picornaviruses	picornavirus	NNS
cord-010188-884d196k	98	8	,	,	,
cord-010188-884d196k	98	9	where	where	WRB
cord-010188-884d196k	98	10	the	the	DT
cord-010188-884d196k	98	11	site	site	NN
cord-010188-884d196k	98	12	of	of	IN
cord-010188-884d196k	98	13	insertion	insertion	NN
cord-010188-884d196k	98	14	was	be	VBD
cord-010188-884d196k	98	15	chosen	choose	VBN
cord-010188-884d196k	98	16	on	on	IN
cord-010188-884d196k	98	17	the	the	DT
cord-010188-884d196k	98	18	basis	basis	NN
cord-010188-884d196k	98	19	of	of	IN
cord-010188-884d196k	98	20	the	the	DT
cord-010188-884d196k	98	21	crystallographic	crystallographic	JJ
cord-010188-884d196k	98	22	structure	structure	NN
cord-010188-884d196k	98	23	of	of	IN
cord-010188-884d196k	98	24	the	the	DT
cord-010188-884d196k	98	25	virus	virus	NN
cord-010188-884d196k	98	26	7	7	CD
cord-010188-884d196k	98	27	.	.	.
cord-010188-884d196k	99	1	However	however	RB
cord-010188-884d196k	99	2	,	,	,
cord-010188-884d196k	99	3	the	the	DT
cord-010188-884d196k	99	4	three	three	CD
cord-010188-884d196k	99	5	-	-	HYPH
cord-010188-884d196k	99	6	dimensional	dimensional	JJ
cord-010188-884d196k	99	7	structures	structure	NNS
cord-010188-884d196k	99	8	of	of	IN
cord-010188-884d196k	99	9	the	the	DT
cord-010188-884d196k	99	10	Sindbis	Sindbis	NNP
cord-010188-884d196k	99	11	virus	virus	NN
cord-010188-884d196k	99	12	glycoproteins	glycoprotein	NNS
cord-010188-884d196k	99	13	are	be	VBP
cord-010188-884d196k	99	14	not	not	RB
cord-010188-884d196k	99	15	known	know	VBN
cord-010188-884d196k	99	16	,	,	,
cord-010188-884d196k	99	17	and	and	CC
cord-010188-884d196k	99	18	therefore	therefore	RB
cord-010188-884d196k	99	19	it	-PRON-	PRP
cord-010188-884d196k	99	20	has	have	VBZ
cord-010188-884d196k	99	21	not	not	RB
cord-010188-884d196k	99	22	been	be	VBN
cord-010188-884d196k	99	23	possible	possible	JJ
cord-010188-884d196k	99	24	to	to	TO
cord-010188-884d196k	99	25	predict	predict	VB
cord-010188-884d196k	99	26	the	the	DT
cord-010188-884d196k	99	27	regions	region	NNS
cord-010188-884d196k	99	28	of	of	IN
cord-010188-884d196k	99	29	the	the	DT
cord-010188-884d196k	99	30	glycoprotein	glycoprotein	NN
cord-010188-884d196k	99	31	which	which	WDT
cord-010188-884d196k	99	32	form	form	NN
cord-010188-884d196k	99	33	surface	surface	NN
cord-010188-884d196k	99	34	loops	loop	NNS
cord-010188-884d196k	99	35	and	and	CC
cord-010188-884d196k	99	36	into	into	IN
cord-010188-884d196k	99	37	which	which	WDT
cord-010188-884d196k	99	38	insertion	insertion	NN
cord-010188-884d196k	99	39	of	of	IN
cord-010188-884d196k	99	40	additional	additional	JJ
cord-010188-884d196k	99	41	sequence	sequence	NN
cord-010188-884d196k	99	42	would	would	MD
cord-010188-884d196k	99	43	not	not	RB
cord-010188-884d196k	99	44	adversely	adversely	RB
cord-010188-884d196k	99	45	affect	affect	VB
cord-010188-884d196k	99	46	glycoprotein	glycoprotein	NN
cord-010188-884d196k	99	47	folding	fold	VBG
cord-010188-884d196k	99	48	.	.	.
cord-010188-884d196k	100	1	Initial	initial	JJ
cord-010188-884d196k	100	2	studies	study	NNS
cord-010188-884d196k	100	3	,	,	,
cord-010188-884d196k	100	4	using	use	VBG
cord-010188-884d196k	100	5	an	an	DT
cord-010188-884d196k	100	6	epitope	epitope	NN
cord-010188-884d196k	100	7	from	from	IN
cord-010188-884d196k	100	8	Rift	Rift	NNP
cord-010188-884d196k	100	9	Valley	Valley	NNP
cord-010188-884d196k	100	10	fever	fever	NN
cord-010188-884d196k	100	11	virus	virus	NN
cord-010188-884d196k	100	12	,	,	,
cord-010188-884d196k	100	13	involved	involve	VBN
cord-010188-884d196k	100	14	making	make	VBG
cord-010188-884d196k	100	15	random	random	JJ
cord-010188-884d196k	100	16	insertions	insertion	NNS
cord-010188-884d196k	100	17	into	into	IN
cord-010188-884d196k	100	18	the	the	DT
cord-010188-884d196k	100	19	structural	structural	JJ
cord-010188-884d196k	100	20	genes	gene	NNS
cord-010188-884d196k	100	21	,	,	,
cord-010188-884d196k	100	22	followed	follow	VBN
cord-010188-884d196k	100	23	by	by	IN
cord-010188-884d196k	100	24	selection	selection	NN
cord-010188-884d196k	100	25	of	of	IN
cord-010188-884d196k	100	26	viable	viable	JJ
cord-010188-884d196k	100	27	viruses	virus	NNS
cord-010188-884d196k	100	28	containing	contain	VBG
cord-010188-884d196k	100	29	the	the	DT
cord-010188-884d196k	100	30	insert	insert	NN
cord-010188-884d196k	100	31	.	.	.
cord-010188-884d196k	101	1	The	the	DT
cord-010188-884d196k	101	2	chimeric	chimeric	JJ
cord-010188-884d196k	101	3	Sindbis	Sindbis	NNP
cord-010188-884d196k	101	4	virus	virus	NN
cord-010188-884d196k	101	5	that	that	WDT
cord-010188-884d196k	101	6	could	could	MD
cord-010188-884d196k	101	7	tolerate	tolerate	VB
cord-010188-884d196k	101	8	insertions	insertion	NNS
cord-010188-884d196k	101	9	and	and	CC
cord-010188-884d196k	101	10	that	that	WDT
cord-010188-884d196k	101	11	displayed	display	VBD
cord-010188-884d196k	101	12	immunogenic	immunogenic	JJ
cord-010188-884d196k	101	13	Rift	Rift	NNP
cord-010188-884d196k	101	14	Valley	Valley	NNP
cord-010188-884d196k	101	15	fever	fever	NN
cord-010188-884d196k	101	16	virus	virus	NN
cord-010188-884d196k	101	17	epitopes	epitope	NNS
cord-010188-884d196k	101	18	was	be	VBD
cord-010188-884d196k	101	19	used	use	VBN
cord-010188-884d196k	101	20	as	as	IN
cord-010188-884d196k	101	21	a	a	DT
cord-010188-884d196k	101	22	vaccine	vaccine	NN
cord-010188-884d196k	101	23	to	to	TO
cord-010188-884d196k	101	24	make	make	VB
cord-010188-884d196k	101	25	mice	mouse	NNS
cord-010188-884d196k	101	26	resistant	resistant	JJ
cord-010188-884d196k	101	27	to	to	IN
cord-010188-884d196k	101	28	infection	infection	NN
cord-010188-884d196k	101	29	by	by	IN
cord-010188-884d196k	101	30	Rift	Rift	NNP
cord-010188-884d196k	101	31	Valley	Valley	NNP
cord-010188-884d196k	101	32	fever	fever	NN
cord-010188-884d196k	101	33	virus	virus	NN
cord-010188-884d196k	101	34	.	.	.
cord-010188-884d196k	102	1	There	there	EX
cord-010188-884d196k	102	2	are	be	VBP
cord-010188-884d196k	102	3	now	now	RB
cord-010188-884d196k	102	4	several	several	JJ
cord-010188-884d196k	102	5	reports	report	NNS
cord-010188-884d196k	102	6	in	in	IN
cord-010188-884d196k	102	7	the	the	DT
cord-010188-884d196k	102	8	literature	literature	NN
cord-010188-884d196k	102	9	in	in	IN
cord-010188-884d196k	102	10	which	which	WDT
cord-010188-884d196k	102	11	the	the	DT
cord-010188-884d196k	102	12	Sindbis	Sindbis	NNP
cord-010188-884d196k	102	13	virus	virus	NN
cord-010188-884d196k	102	14	vectors	vector	NNS
cord-010188-884d196k	102	15	have	have	VBP
cord-010188-884d196k	102	16	provided	provide	VBN
cord-010188-884d196k	102	17	an	an	DT
cord-010188-884d196k	102	18	The	the	DT
cord-010188-884d196k	102	19	double	double	JJ
cord-010188-884d196k	102	20	subgenomic	subgenomic	NN
cord-010188-884d196k	102	21	RNA	RNA	NNP
cord-010188-884d196k	102	22	Sindbis	Sindbis	NNP
cord-010188-884d196k	102	23	viruses	virus	NNS
cord-010188-884d196k	102	24	have	have	VBP
cord-010188-884d196k	102	25	been	be	VBN
cord-010188-884d196k	102	26	used	use	VBN
cord-010188-884d196k	102	27	in	in	IN
cord-010188-884d196k	102	28	two	two	CD
cord-010188-884d196k	102	29	studies	study	NNS
cord-010188-884d196k	102	30	.	.	.
cord-010188-884d196k	103	1	Hahn	Hahn	NNP
cord-010188-884d196k	103	2	et	et	NNP
cord-010188-884d196k	103	3	al	al	NNP
cord-010188-884d196k	103	4	.	.	.
cord-010188-884d196k	104	1	16	16	CD
cord-010188-884d196k	104	2	inserted	insert	VBD
cord-010188-884d196k	104	3	either	either	CC
cord-010188-884d196k	104	4	a	a	DT
cord-010188-884d196k	104	5	truncated	truncated	JJ
cord-010188-884d196k	104	6	form	form	NN
cord-010188-884d196k	104	7	of	of	IN
cord-010188-884d196k	104	8	the	the	DT
cord-010188-884d196k	104	9	influenza	influenza	NN
cord-010188-884d196k	104	10	hemagglutinin	hemagglutinin	NN
cord-010188-884d196k	104	11	(	(	-LRB-
cord-010188-884d196k	104	12	HA	HA	NNP
cord-010188-884d196k	104	13	)	)	-RRB-
cord-010188-884d196k	104	14	protein	protein	NN
cord-010188-884d196k	104	15	,	,	,
cord-010188-884d196k	104	16	or	or	CC
cord-010188-884d196k	104	17	minigenes	minigene	NNS
cord-010188-884d196k	104	18	encoding	encode	VBG
cord-010188-884d196k	104	19	immunodominant	immunodominant	JJ
cord-010188-884d196k	104	20	cytotoxic	cytotoxic	JJ
cord-010188-884d196k	104	21	T	t	NN
cord-010188-884d196k	104	22	-	-	HYPH
cord-010188-884d196k	104	23	cell	cell	NN
cord-010188-884d196k	104	24	epitopes	epitope	NNS
cord-010188-884d196k	104	25	into	into	IN
cord-010188-884d196k	104	26	the	the	DT
cord-010188-884d196k	104	27	Sindbis	Sindbis	NNP
cord-010188-884d196k	104	28	virus	virus	NN
cord-010188-884d196k	104	29	genome	genome	NN
cord-010188-884d196k	104	30	.	.	.
cord-010188-884d196k	105	1	These	these	DT
cord-010188-884d196k	105	2	vectors	vector	NNS
cord-010188-884d196k	105	3	were	be	VBD
cord-010188-884d196k	105	4	used	use	VBN
cord-010188-884d196k	105	5	to	to	TO
cord-010188-884d196k	105	6	sensitize	sensitize	VB
cord-010188-884d196k	105	7	target	target	NN
cord-010188-884d196k	105	8	cells	cell	NNS
cord-010188-884d196k	105	9	for	for	IN
cord-010188-884d196k	105	10	lysis	lysis	NN
cord-010188-884d196k	105	11	by	by	IN
cord-010188-884d196k	105	12	appropriate	appropriate	JJ
cord-010188-884d196k	105	13	major	major	JJ
cord-010188-884d196k	105	14	histocompatibility	histocompatibility	NN
cord-010188-884d196k	105	15	complex	complex	NN
cord-010188-884d196k	105	16	-	-	HYPH
cord-010188-884d196k	105	17	restricted	restricted	JJ
cord-010188-884d196k	105	18	,	,	,
cord-010188-884d196k	105	19	HA	HA	NNP
cord-010188-884d196k	105	20	-	-	HYPH
cord-010188-884d196k	105	21	specific	specific	JJ
cord-010188-884d196k	105	22	,	,	,
cord-010188-884d196k	105	23	cytotoxic	cytotoxic	JJ
cord-010188-884d196k	105	24	T	t	NN
cord-010188-884d196k	105	25	cells	cell	NNS
cord-010188-884d196k	105	26	.	.	.
cord-010188-884d196k	106	1	They	-PRON-	PRP
cord-010188-884d196k	106	2	were	be	VBD
cord-010188-884d196k	106	3	also	also	RB
cord-010188-884d196k	106	4	used	use	VBN
cord-010188-884d196k	106	5	to	to	TO
cord-010188-884d196k	106	6	infect	infect	VB
cord-010188-884d196k	106	7	mice	mouse	NNS
cord-010188-884d196k	106	8	to	to	TO
cord-010188-884d196k	106	9	prime	prime	VB
cord-010188-884d196k	106	10	a	a	DT
cord-010188-884d196k	106	11	specific	specific	JJ
cord-010188-884d196k	106	12	T	t	NN
cord-010188-884d196k	106	13	-	-	HYPH
cord-010188-884d196k	106	14	cell	cell	NN
cord-010188-884d196k	106	15	response	response	NN
cord-010188-884d196k	106	16	.	.	.
cord-010188-884d196k	107	1	In	in	IN
cord-010188-884d196k	107	2	addition	addition	NN
cord-010188-884d196k	107	3	,	,	,
cord-010188-884d196k	107	4	the	the	DT
cord-010188-884d196k	107	5	double	double	JJ
cord-010188-884d196k	107	6	subgenorrfic	subgenorrfic	NN
cord-010188-884d196k	107	7	1KNA	1kna	CD
cord-010188-884d196k	107	8	vector	vector	NN
cord-010188-884d196k	107	9	was	be	VBD
cord-010188-884d196k	107	10	used	use	VBN
cord-010188-884d196k	107	11	to	to	TO
cord-010188-884d196k	107	12	express	express	VB
cord-010188-884d196k	107	13	the	the	DT
cord-010188-884d196k	107	14	insulin	insulin	NN
cord-010188-884d196k	107	15	-	-	HYPH
cord-010188-884d196k	107	16	regulated	regulate	VBN
cord-010188-884d196k	107	17	glucose	glucose	NN
cord-010188-884d196k	107	18	-	-	HYPH
cord-010188-884d196k	107	19	transporter	transporter	NN
cord-010188-884d196k	107	20	protein	protein	NN
cord-010188-884d196k	107	21	in	in	IN
cord-010188-884d196k	107	22	cultured	cultured	JJ
cord-010188-884d196k	107	23	cells2L	cells2L	NNS
cord-010188-884d196k	108	1	This	this	DT
cord-010188-884d196k	108	2	protein	protein	NN
cord-010188-884d196k	108	3	,	,	,
cord-010188-884d196k	108	4	when	when	WRB
cord-010188-884d196k	108	5	expressed	express	VBN
cord-010188-884d196k	108	6	by	by	IN
cord-010188-884d196k	108	7	the	the	DT
cord-010188-884d196k	108	8	Sindbis	Sindbis	NNP
cord-010188-884d196k	108	9	vector	vector	NN
cord-010188-884d196k	108	10	,	,	,
cord-010188-884d196k	108	11	was	be	VBD
cord-010188-884d196k	108	12	found	find	VBN
cord-010188-884d196k	108	13	in	in	IN
cord-010188-884d196k	108	14	adipocytes	adipocyte	NNS
cord-010188-884d196k	108	15	and	and	CC
cord-010188-884d196k	108	16	muscle	muscle	NN
cord-010188-884d196k	108	17	cells	cell	NNS
cord-010188-884d196k	108	18	-its	-its	:
cord-010188-884d196k	108	19	normal	normal	JJ
cord-010188-884d196k	108	20	intracellular	intracellular	JJ
cord-010188-884d196k	108	21	location	location	NN
cord-010188-884d196k	108	22	.	.	.
cord-010188-884d196k	109	1	The	the	DT
cord-010188-884d196k	109	2	Semliki	Semliki	NNP
cord-010188-884d196k	109	3	Forest	Forest	NNP
cord-010188-884d196k	109	4	virus	virus	NN
cord-010188-884d196k	109	5	vector	vector	NN
cord-010188-884d196k	109	6	has	have	VBZ
cord-010188-884d196k	109	7	been	be	VBN
cord-010188-884d196k	109	8	used	use	VBN
cord-010188-884d196k	109	9	to	to	TO
cord-010188-884d196k	109	10	express	express	VB
cord-010188-884d196k	109	11	a	a	DT
cord-010188-884d196k	109	12	variety	variety	NN
cord-010188-884d196k	109	13	of	of	IN
cord-010188-884d196k	109	14	proteins	protein	NNS
cord-010188-884d196k	109	15	,	,	,
cord-010188-884d196k	109	16	including	include	VBG
cord-010188-884d196k	109	17	dihydrofolate	dihydrofolate	NN
cord-010188-884d196k	109	18	reductase	reductase	NN
cord-010188-884d196k	109	19	(	(	-LRB-
cord-010188-884d196k	109	20	DHFR	DHFR	NNP
cord-010188-884d196k	109	21	)	)	-RRB-
cord-010188-884d196k	109	22	,	,	,
cord-010188-884d196k	109	23	the	the	DT
cord-010188-884d196k	109	24	transferrin	transferrin	NN
cord-010188-884d196k	109	25	receptor	receptor	NN
cord-010188-884d196k	109	26	,	,	,
cord-010188-884d196k	109	27	and	and	CC
cord-010188-884d196k	109	28	lysozyme	lysozyme	NNS
cord-010188-884d196k	109	29	,	,	,
cord-010188-884d196k	109	30	proving	prove	VBG
cord-010188-884d196k	109	31	that	that	IN
cord-010188-884d196k	109	32	the	the	DT
cord-010188-884d196k	109	33	vector	vector	NN
cord-010188-884d196k	109	34	can	can	MD
cord-010188-884d196k	109	35	be	be	VB
cord-010188-884d196k	109	36	used	use	VBN
cord-010188-884d196k	109	37	to	to	TO
cord-010188-884d196k	109	38	express	express	VB
cord-010188-884d196k	109	39	cytoplasmic	cytoplasmic	JJ
cord-010188-884d196k	109	40	,	,	,
cord-010188-884d196k	109	41	membrane	membrane	NN
cord-010188-884d196k	109	42	and	and	CC
cord-010188-884d196k	109	43	secreted	secrete	VBN
cord-010188-884d196k	109	44	proteins	protein	NNS
cord-010188-884d196k	109	45	,	,	,
cord-010188-884d196k	109	46	respectively	respectively	RB
cord-010188-884d196k	109	47	is	be	VBZ
cord-010188-884d196k	109	48	.	.	.
cord-010188-884d196k	110	1	Studies	study	NNS
cord-010188-884d196k	110	2	on	on	IN
cord-010188-884d196k	110	3	the	the	DT
cord-010188-884d196k	110	4	expression	expression	NN
cord-010188-884d196k	110	5	of	of	IN
cord-010188-884d196k	110	6	~3-galactosidase	~3-galactosidase	NFP
cord-010188-884d196k	110	7	indicated	indicate	VBD
cord-010188-884d196k	110	8	that	that	IN
cord-010188-884d196k	110	9	about	about	IN
cord-010188-884d196k	110	10	400txg	400txg	CD
cord-010188-884d196k	110	11	of	of	IN
cord-010188-884d196k	110	12	the	the	DT
cord-010188-884d196k	110	13	heterologous	heterologous	JJ
cord-010188-884d196k	110	14	protein	protein	NN
cord-010188-884d196k	110	15	could	could	MD
cord-010188-884d196k	110	16	be	be	VB
cord-010188-884d196k	110	17	produced	produce	VBN
cord-010188-884d196k	110	18	from	from	IN
cord-010188-884d196k	110	19	5x	5x	CD
cord-010188-884d196k	110	20	106	106	CD
cord-010188-884d196k	110	21	cells	cell	NNS
cord-010188-884d196k	110	22	,	,	,
cord-010188-884d196k	110	23	corresponding	correspond	VBG
cord-010188-884d196k	110	24	to	to	IN
cord-010188-884d196k	110	25	approximately	approximately	RB
cord-010188-884d196k	110	26	25	25	CD
cord-010188-884d196k	110	27	%	%	NN
cord-010188-884d196k	110	28	of	of	IN
cord-010188-884d196k	110	29	total	total	JJ
cord-010188-884d196k	110	30	cellular	cellular	JJ
cord-010188-884d196k	110	31	protein	protein	NN
cord-010188-884d196k	110	32	is	be	VBZ
cord-010188-884d196k	110	33	.	.	.
cord-010188-884d196k	111	1	The	the	DT
cord-010188-884d196k	111	2	initial	initial	JJ
cord-010188-884d196k	111	3	studies	study	NNS
cord-010188-884d196k	111	4	with	with	IN
cord-010188-884d196k	111	5	Sindbis	Sindbis	NNP
cord-010188-884d196k	111	6	and	and	CC
cord-010188-884d196k	111	7	Semliki	Semliki	NNP
cord-010188-884d196k	111	8	Forest	Forest	NNP
cord-010188-884d196k	111	9	virus	virus	NN
cord-010188-884d196k	111	10	suggest	suggest	VBP
cord-010188-884d196k	111	11	that	that	IN
cord-010188-884d196k	111	12	both	both	DT
cord-010188-884d196k	111	13	viruses	virus	NNS
cord-010188-884d196k	111	14	are	be	VBP
cord-010188-884d196k	111	15	promising	promise	VBG
cord-010188-884d196k	111	16	as	as	IN
cord-010188-884d196k	111	17	vectors	vector	NNS
cord-010188-884d196k	111	18	for	for	IN
cord-010188-884d196k	111	19	heterologous	heterologous	JJ
cord-010188-884d196k	111	20	gene	gene	NN
cord-010188-884d196k	111	21	expression	expression	NN
cord-010188-884d196k	111	22	.	.	.
cord-010188-884d196k	112	1	The	the	DT
cord-010188-884d196k	112	2	potential	potential	NN
cord-010188-884d196k	112	3	of	of	IN
cord-010188-884d196k	112	4	using	use	VBG
cord-010188-884d196k	112	5	alphaviruses	alphavirus	NNS
cord-010188-884d196k	112	6	to	to	TO
cord-010188-884d196k	112	7	stimulate	stimulate	VB
cord-010188-884d196k	112	8	production	production	NN
cord-010188-884d196k	112	9	of	of	IN
cord-010188-884d196k	112	10	specific	specific	JJ
cord-010188-884d196k	112	11	subsets	subset	NNS
cord-010188-884d196k	112	12	of	of	IN
cord-010188-884d196k	112	13	T	t	NN
cord-010188-884d196k	112	14	cells	cell	NNS
cord-010188-884d196k	112	15	by	by	IN
cord-010188-884d196k	112	16	infection	infection	NN
cord-010188-884d196k	112	17	of	of	IN
cord-010188-884d196k	112	18	animals	animal	NNS
cord-010188-884d196k	112	19	is	be	VBZ
cord-010188-884d196k	112	20	only	only	RB
cord-010188-884d196k	112	21	just	just	RB
cord-010188-884d196k	112	22	beginning	begin	VBG
cord-010188-884d196k	112	23	to	to	TO
cord-010188-884d196k	112	24	be	be	VB
cord-010188-884d196k	112	25	explored	explore	VBN
cord-010188-884d196k	112	26	.	.	.
cord-010188-884d196k	113	1	The	the	DT
cord-010188-884d196k	113	2	use	use	NN
cord-010188-884d196k	113	3	of	of	IN
cord-010188-884d196k	113	4	alphaviruses	alphavirus	NNS
cord-010188-884d196k	113	5	to	to	TO
cord-010188-884d196k	113	6	express	express	VB
cord-010188-884d196k	113	7	proteins	protein	NNS
cord-010188-884d196k	113	8	for	for	IN
cord-010188-884d196k	113	9	production	production	NN
cord-010188-884d196k	113	10	is	be	VBZ
cord-010188-884d196k	113	11	ready	ready	JJ
cord-010188-884d196k	113	12	to	to	TO
cord-010188-884d196k	113	13	be	be	VB
cord-010188-884d196k	113	14	exploited	exploit	VBN
cord-010188-884d196k	113	15	.	.	.
cord-010188-884d196k	114	1	One	one	CD
cord-010188-884d196k	114	2	particular	particular	JJ
cord-010188-884d196k	114	3	aspect	aspect	NN
cord-010188-884d196k	114	4	of	of	IN
cord-010188-884d196k	114	5	using	use	VBG
cord-010188-884d196k	114	6	alphaviruses	alphavirus	NNS
cord-010188-884d196k	114	7	as	as	IN
cord-010188-884d196k	114	8	vectors	vector	NNS
cord-010188-884d196k	114	9	needs	need	VBZ
cord-010188-884d196k	114	10	further	further	JJ
cord-010188-884d196k	114	11	investigation	investigation	NN
cord-010188-884d196k	114	12	.	.	.
cord-010188-884d196k	115	1	Infection	infection	NN
cord-010188-884d196k	115	2	by	by	IN
cord-010188-884d196k	115	3	these	these	DT
cord-010188-884d196k	115	4	viruses	virus	NNS
cord-010188-884d196k	115	5	is	be	VBZ
cord-010188-884d196k	115	6	cytopathic	cytopathic	JJ
cord-010188-884d196k	115	7	for	for	IN
cord-010188-884d196k	115	8	most	most	JJS
cord-010188-884d196k	115	9	vertebrate	vertebrate	JJ
cord-010188-884d196k	115	10	cells	cell	NNS
cord-010188-884d196k	115	11	that	that	WDT
cord-010188-884d196k	115	12	are	be	VBP
cord-010188-884d196k	115	13	grown	grow	VBN
cord-010188-884d196k	115	14	in	in	IN
cord-010188-884d196k	115	15	culture	culture	NN
cord-010188-884d196k	115	16	.	.	.
cord-010188-884d196k	116	1	There	there	EX
cord-010188-884d196k	116	2	are	be	VBP
cord-010188-884d196k	116	3	some	some	DT
cord-010188-884d196k	116	4	variants	variant	NNS
cord-010188-884d196k	116	5	that	that	WDT
cord-010188-884d196k	116	6	seem	seem	VBP
cord-010188-884d196k	116	7	to	to	TO
cord-010188-884d196k	116	8	be	be	VB
cord-010188-884d196k	116	9	much	much	RB
cord-010188-884d196k	116	10	less	less	RBR
cord-010188-884d196k	116	11	cytopathic	cytopathic	JJ
cord-010188-884d196k	116	12	and	and	CC
cord-010188-884d196k	116	13	,	,	,
cord-010188-884d196k	116	14	for	for	IN
cord-010188-884d196k	116	15	most	most	JJS
cord-010188-884d196k	116	16	of	of	IN
cord-010188-884d196k	116	17	the	the	DT
cord-010188-884d196k	116	18	Sindbis	Sindbis	NNP
cord-010188-884d196k	116	19	virus	virus	NN
cord-010188-884d196k	116	20	variants	variant	NNS
cord-010188-884d196k	116	21	,	,	,
cord-010188-884d196k	116	22	infected	infected	JJ
cord-010188-884d196k	116	23	mosquito	mosquito	NN
cord-010188-884d196k	116	24	cells	cell	NNS
cord-010188-884d196k	116	25	in	in	IN
cord-010188-884d196k	116	26	culture	culture	NN
cord-010188-884d196k	116	27	do	do	VBP
cord-010188-884d196k	116	28	not	not	RB
cord-010188-884d196k	116	29	show	show	VB
cord-010188-884d196k	116	30	cytopathic	cytopathic	JJ
cord-010188-884d196k	116	31	effects	effect	NNS
cord-010188-884d196k	116	32	22	22	CD
cord-010188-884d196k	116	33	.	.	.
cord-010188-884d196k	117	1	The	the	DT
cord-010188-884d196k	117	2	mechanisms	mechanism	NNS
cord-010188-884d196k	117	3	of	of	IN
cord-010188-884d196k	117	4	cell	cell	NN
cord-010188-884d196k	117	5	killing	killing	NN
cord-010188-884d196k	117	6	are	be	VBP
cord-010188-884d196k	117	7	not	not	RB
cord-010188-884d196k	117	8	known	know	VBN
cord-010188-884d196k	117	9	.	.	.
cord-010188-884d196k	118	1	Infection	infection	NN
cord-010188-884d196k	118	2	of	of	IN
cord-010188-884d196k	118	3	cultured	cultured	JJ
cord-010188-884d196k	118	4	cells	cell	NNS
cord-010188-884d196k	118	5	with	with	IN
cord-010188-884d196k	118	6	the	the	DT
cord-010188-884d196k	118	7	Semliki	Semliki	NNP
cord-010188-884d196k	118	8	Forest	Forest	NNP
cord-010188-884d196k	118	9	virus	virus	NN
cord-010188-884d196k	118	10	vectors	vector	VBZ
cord-010188-884d196k	118	11	shuts	shut	VBZ
cord-010188-884d196k	118	12	offthe	offthe	NNP
cord-010188-884d196k	118	13	synthesis	synthesis	NN
cord-010188-884d196k	118	14	of	of	IN
cord-010188-884d196k	118	15	host	host	NN
cord-010188-884d196k	118	16	-	-	HYPH
cord-010188-884d196k	118	17	cell	cell	NN
cord-010188-884d196k	118	18	proteins	protein	NNS
cord-010188-884d196k	118	19	,	,	,
cord-010188-884d196k	118	20	demonstrating	demonstrate	VBG
cord-010188-884d196k	118	21	that	that	IN
cord-010188-884d196k	118	22	the	the	DT
cord-010188-884d196k	118	23	viral	viral	JJ
cord-010188-884d196k	118	24	structural	structural	JJ
cord-010188-884d196k	118	25	proteins	protein	NNS
cord-010188-884d196k	118	26	are	be	VBP
cord-010188-884d196k	118	27	not	not	RB
cord-010188-884d196k	118	28	required	require	VBN
cord-010188-884d196k	118	29	for	for	IN
cord-010188-884d196k	118	30	this	this	DT
cord-010188-884d196k	118	31	effect	effect	NN
cord-010188-884d196k	118	32	15	15	CD
cord-010188-884d196k	118	33	.	.	.
cord-010188-884d196k	119	1	The	the	DT
cord-010188-884d196k	119	2	inhibition	inhibition	NN
cord-010188-884d196k	119	3	of	of	IN
cord-010188-884d196k	119	4	host	host	NN
cord-010188-884d196k	119	5	-	-	HYPH
cord-010188-884d196k	119	6	cell	cell	NN
cord-010188-884d196k	119	7	protein	protein	NN
cord-010188-884d196k	119	8	synthesis	synthesis	NN
cord-010188-884d196k	119	9	may	may	MD
cord-010188-884d196k	119	10	be	be	VB
cord-010188-884d196k	119	11	due	due	JJ
cord-010188-884d196k	119	12	to	to	IN
cord-010188-884d196k	119	13	the	the	DT
cord-010188-884d196k	119	14	expression	expression	NN
cord-010188-884d196k	119	15	of	of	IN
cord-010188-884d196k	119	16	a	a	DT
cord-010188-884d196k	119	17	particular	particular	JJ
cord-010188-884d196k	119	18	viral	viral	JJ
cord-010188-884d196k	119	19	gene	gene	NN
cord-010188-884d196k	119	20	product	product	NN
cord-010188-884d196k	119	21	,	,	,
cord-010188-884d196k	119	22	or	or	CC
cord-010188-884d196k	119	23	it	-PRON-	PRP
cord-010188-884d196k	119	24	could	could	MD
cord-010188-884d196k	119	25	be	be	VB
cord-010188-884d196k	119	26	a	a	DT
cord-010188-884d196k	119	27	consequence	consequence	NN
cord-010188-884d196k	119	28	of	of	IN
cord-010188-884d196k	119	29	the	the	DT
cord-010188-884d196k	119	30	very	very	RB
cord-010188-884d196k	119	31	high	high	JJ
cord-010188-884d196k	119	32	level	level	NN
cord-010188-884d196k	119	33	of	of	IN
cord-010188-884d196k	119	34	expression	expression	NN
cord-010188-884d196k	119	35	of	of	IN
cord-010188-884d196k	119	36	the	the	DT
cord-010188-884d196k	119	37	heterologous	heterologous	JJ
cord-010188-884d196k	119	38	gene	gene	NN
cord-010188-884d196k	119	39	.	.	.
cord-010188-884d196k	120	1	It	-PRON-	PRP
cord-010188-884d196k	120	2	is	be	VBZ
cord-010188-884d196k	120	3	possible	possible	JJ
cord-010188-884d196k	120	4	to	to	TO
cord-010188-884d196k	120	5	modulate	modulate	VB
cord-010188-884d196k	120	6	the	the	DT
cord-010188-884d196k	120	7	expression	expression	NN
cord-010188-884d196k	120	8	of	of	IN
cord-010188-884d196k	120	9	the	the	DT
cord-010188-884d196k	120	10	subgenomic	subgenomic	NNP
cord-010188-884d196k	120	11	RNA	RNA	NNP
cord-010188-884d196k	120	12	by	by	IN
cord-010188-884d196k	120	13	the	the	DT
cord-010188-884d196k	120	14	use	use	NN
cord-010188-884d196k	120	15	of	of	IN
cord-010188-884d196k	120	16	temperature	temperature	NN
cord-010188-884d196k	120	17	-	-	HYPH
cord-010188-884d196k	120	18	sensitive	sensitive	JJ
cord-010188-884d196k	120	19	mutants	mutant	NNS
cord-010188-884d196k	120	20	or	or	CC
cord-010188-884d196k	120	21	by	by	IN
cord-010188-884d196k	120	22	the	the	DT
cord-010188-884d196k	120	23	down	down	JJ
cord-010188-884d196k	120	24	-	-	HYPH
cord-010188-884d196k	120	25	regulation	regulation	NN
cord-010188-884d196k	120	26	of	of	IN
cord-010188-884d196k	120	27	the	the	DT
cord-010188-884d196k	120	28	subgenomic	subgenomic	NNP
cord-010188-884d196k	120	29	RNA	RNA	NNP
cord-010188-884d196k	120	30	promoter	promoter	NN
cord-010188-884d196k	120	31	~3	~3	NFP
cord-010188-884d196k	120	32	.	.	.
cord-010188-884d196k	121	1	A	a	DT
cord-010188-884d196k	121	2	second	second	JJ
cord-010188-884d196k	121	3	issue	issue	NN
cord-010188-884d196k	121	4	of	of	IN
cord-010188-884d196k	121	5	importance	importance	NN
cord-010188-884d196k	121	6	is	be	VBZ
cord-010188-884d196k	121	7	the	the	DT
cord-010188-884d196k	121	8	possible	possible	JJ
cord-010188-884d196k	121	9	biohazard	biohazard	NN
cord-010188-884d196k	121	10	of	of	IN
cord-010188-884d196k	121	11	working	work	VBG
cord-010188-884d196k	121	12	with	with	IN
cord-010188-884d196k	121	13	alphaviruses	alphavirus	NNS
cord-010188-884d196k	121	14	.	.	.
cord-010188-884d196k	122	1	The	the	DT
cord-010188-884d196k	122	2	vectors	vector	NNS
cord-010188-884d196k	122	3	derived	derive	VBN
cord-010188-884d196k	122	4	from	from	IN
cord-010188-884d196k	122	5	Sernliki	Sernliki	NNP
cord-010188-884d196k	122	6	Forest	Forest	NNP
cord-010188-884d196k	122	7	virus	virus	NN
cord-010188-884d196k	122	8	that	that	WDT
cord-010188-884d196k	122	9	are	be	VBP
cord-010188-884d196k	122	10	unable	unable	JJ
cord-010188-884d196k	122	11	to	to	TO
cord-010188-884d196k	122	12	provide	provide	VB
cord-010188-884d196k	122	13	any	any	DT
cord-010188-884d196k	122	14	viral	viral	JJ
cord-010188-884d196k	122	15	structural	structural	JJ
cord-010188-884d196k	122	16	proteins	protein	NNS
cord-010188-884d196k	122	17	(	(	-LRB-
cord-010188-884d196k	122	18	termed	term	VBN
cord-010188-884d196k	122	19	'	'	``
cord-010188-884d196k	122	20	suicide	suicide	NN
cord-010188-884d196k	122	21	'	'	''
cord-010188-884d196k	122	22	vectors	vector	NNS
cord-010188-884d196k	122	23	)	)	-RRB-
cord-010188-884d196k	122	24	should	should	MD
cord-010188-884d196k	122	25	be	be	VB
cord-010188-884d196k	122	26	safe	safe	JJ
cord-010188-884d196k	122	27	for	for	IN
cord-010188-884d196k	122	28	laboratory	laboratory	NN
cord-010188-884d196k	122	29	procedures	procedure	NNS
cord-010188-884d196k	122	30	,	,	,
cord-010188-884d196k	122	31	although	although	IN
cord-010188-884d196k	122	32	the	the	DT
cord-010188-884d196k	122	33	possibility	possibility	NN
cord-010188-884d196k	122	34	of	of	IN
cord-010188-884d196k	122	35	recombination	recombination	NN
cord-010188-884d196k	122	36	and	and	CC
cord-010188-884d196k	122	37	the	the	DT
cord-010188-884d196k	122	38	production	production	NN
cord-010188-884d196k	122	39	of	of	IN
cord-010188-884d196k	122	40	segmented	segmented	JJ
cord-010188-884d196k	122	41	genome	genome	NN
cord-010188-884d196k	122	42	particles	particle	NNS
cord-010188-884d196k	122	43	has	have	VBZ
cord-010188-884d196k	122	44	not	not	RB
cord-010188-884d196k	122	45	been	be	VBN
cord-010188-884d196k	122	46	fully	fully	RB
cord-010188-884d196k	122	47	resolved	resolve	VBN
cord-010188-884d196k	122	48	.	.	.
cord-010188-884d196k	123	1	There	there	EX
cord-010188-884d196k	123	2	is	be	VBZ
cord-010188-884d196k	123	3	one	one	CD
cord-010188-884d196k	123	4	report	report	NN
cord-010188-884d196k	123	5	of	of	IN
cord-010188-884d196k	123	6	a	a	DT
cord-010188-884d196k	123	7	fatal	fatal	JJ
cord-010188-884d196k	123	8	infection	infection	NN
cord-010188-884d196k	123	9	by	by	IN
cord-010188-884d196k	123	10	Semliki	Semliki	NNP
cord-010188-884d196k	123	11	Forest	Forest	NNP
cord-010188-884d196k	123	12	virus	virus	NN
cord-010188-884d196k	123	13	23	23	CD
cord-010188-884d196k	123	14	,	,	,
cord-010188-884d196k	123	15	so	so	CC
cord-010188-884d196k	123	16	caution	caution	NN
cord-010188-884d196k	123	17	in	in	IN
cord-010188-884d196k	123	18	using	use	VBG
cord-010188-884d196k	123	19	this	this	DT
cord-010188-884d196k	123	20	virus	virus	NN
cord-010188-884d196k	123	21	must	must	MD
cord-010188-884d196k	123	22	be	be	VB
cord-010188-884d196k	123	23	maintained	maintain	VBN
cord-010188-884d196k	123	24	.	.	.
cord-010188-884d196k	124	1	Most	Most	JJS
cord-010188-884d196k	124	2	strains	strain	NNS
cord-010188-884d196k	124	3	of	of	IN
cord-010188-884d196k	124	4	Sindbis	Sindbis	NNP
cord-010188-884d196k	124	5	virus	virus	NN
cord-010188-884d196k	124	6	are	be	VBP
cord-010188-884d196k	124	7	not	not	RB
cord-010188-884d196k	124	8	pathogenic	pathogenic	JJ
cord-010188-884d196k	124	9	and	and	CC
cord-010188-884d196k	124	10	so	so	RB
cord-010188-884d196k	124	11	they	-PRON-	PRP
cord-010188-884d196k	124	12	may	may	MD
cord-010188-884d196k	124	13	be	be	VB
cord-010188-884d196k	124	14	preferred	prefer	VBN
cord-010188-884d196k	124	15	for	for	IN
cord-010188-884d196k	124	16	many	many	JJ
cord-010188-884d196k	124	17	purposes	purpose	NNS
cord-010188-884d196k	124	18	,	,	,
cord-010188-884d196k	124	19	particularly	particularly	RB
cord-010188-884d196k	124	20	for	for	IN
cord-010188-884d196k	124	21	those	those	DT
cord-010188-884d196k	124	22	investigators	investigator	NNS
cord-010188-884d196k	124	23	who	who	WP
cord-010188-884d196k	124	24	are	be	VBP
cord-010188-884d196k	124	25	less	less	RBR
cord-010188-884d196k	124	26	familiar	familiar	JJ
cord-010188-884d196k	124	27	with	with	IN
cord-010188-884d196k	124	28	working	work	VBG
cord-010188-884d196k	124	29	with	with	IN
cord-010188-884d196k	124	30	infectious	infectious	JJ
cord-010188-884d196k	124	31	agents	agent	NNS
cord-010188-884d196k	124	32	.	.	.
cord-010188-884d196k	125	1	There	there	EX
cord-010188-884d196k	125	2	are	be	VBP
cord-010188-884d196k	125	3	many	many	JJ
cord-010188-884d196k	125	4	ways	way	NNS
cord-010188-884d196k	125	5	that	that	WDT
cord-010188-884d196k	125	6	these	these	DT
cord-010188-884d196k	125	7	viruses	virus	NNS
cord-010188-884d196k	125	8	can	can	MD
cord-010188-884d196k	125	9	be	be	VB
cord-010188-884d196k	125	10	attenuated	attenuate	VBN
cord-010188-884d196k	125	11	and	and	CC
cord-010188-884d196k	125	12	made	make	VBN
cord-010188-884d196k	125	13	safe	safe	JJ
cord-010188-884d196k	125	14	for	for	IN
cord-010188-884d196k	125	15	routine	routine	JJ
cord-010188-884d196k	125	16	work	work	NN
cord-010188-884d196k	125	17	,	,	,
cord-010188-884d196k	125	18	so	so	IN
cord-010188-884d196k	125	19	that	that	IN
cord-010188-884d196k	125	20	in	in	IN
cord-010188-884d196k	125	21	the	the	DT
cord-010188-884d196k	125	22	future	future	NN
cord-010188-884d196k	125	23	their	-PRON-	PRP$
cord-010188-884d196k	125	24	use	use	NN
cord-010188-884d196k	125	25	should	should	MD
cord-010188-884d196k	125	26	become	become	VB
cord-010188-884d196k	125	27	essentially	essentially	RB
cord-010188-884d196k	125	28	risk	risk	NN
cord-010188-884d196k	125	29	-	-	HYPH
cord-010188-884d196k	125	30	free	free	JJ
cord-010188-884d196k	125	31	.	.	.
cord-010188-884d196k	126	1	The	the	DT
cord-010188-884d196k	126	2	initial	initial	JJ
cord-010188-884d196k	126	3	studies	study	NNS
cord-010188-884d196k	126	4	on	on	IN
cord-010188-884d196k	126	5	the	the	DT
cord-010188-884d196k	126	6	development	development	NN
cord-010188-884d196k	126	7	of	of	IN
cord-010188-884d196k	126	8	alphaviruses	alphaviruses	NNP
cord-010188-884d196k	126	9	as	as	IN
cord-010188-884d196k	126	10	vectors	vector	NNS
cord-010188-884d196k	126	11	uncovered	uncover	VBD
cord-010188-884d196k	126	12	the	the	DT
cord-010188-884d196k	126	13	phenomenon	phenomenon	NN
cord-010188-884d196k	126	14	of	of	IN
cord-010188-884d196k	126	15	recombination	recombination	NN
cord-010188-884d196k	126	16	.	.	.
cord-010188-884d196k	127	1	It	-PRON-	PRP
cord-010188-884d196k	127	2	also	also	RB
cord-010188-884d196k	127	3	led	lead	VBD
cord-010188-884d196k	127	4	to	to	IN
cord-010188-884d196k	127	5	some	some	DT
cord-010188-884d196k	127	6	important	important	JJ
cord-010188-884d196k	127	7	discoveries	discovery	NNS
cord-010188-884d196k	127	8	about	about	IN
cord-010188-884d196k	127	9	these	these	DT
cord-010188-884d196k	127	10	viruses	virus	NNS
cord-010188-884d196k	127	11	(	(	-LRB-
cord-010188-884d196k	127	12	such	such	JJ
cord-010188-884d196k	127	13	as	as	IN
cord-010188-884d196k	127	14	the	the	DT
cord-010188-884d196k	127	15	identification	identification	NN
cord-010188-884d196k	127	16	of	of	IN
cord-010188-884d196k	127	17	the	the	DT
cord-010188-884d196k	127	18	packaging	packaging	NN
cord-010188-884d196k	127	19	signal	signal	NN
cord-010188-884d196k	127	20	and	and	CC
cord-010188-884d196k	127	21	a	a	DT
cord-010188-884d196k	127	22	definition	definition	NN
cord-010188-884d196k	127	23	of	of	IN
cord-010188-884d196k	127	24	the	the	DT
cord-010188-884d196k	127	25	subgenomic	subgenomic	NNP
cord-010188-884d196k	127	26	RNA	RNA	NNP
cord-010188-884d196k	127	27	promoter	promoter	NN
cord-010188-884d196k	127	28	)	)	-RRB-
cord-010188-884d196k	127	29	.	.	.
cord-010188-884d196k	128	1	In	in	IN
cord-010188-884d196k	128	2	the	the	DT
cord-010188-884d196k	128	3	future	future	NN
cord-010188-884d196k	128	4	,	,	,
cord-010188-884d196k	128	5	continued	continue	VBD
cord-010188-884d196k	128	6	work	work	NN
cord-010188-884d196k	128	7	on	on	IN
cord-010188-884d196k	128	8	these	these	DT
cord-010188-884d196k	128	9	vectors	vector	NNS
cord-010188-884d196k	128	10	should	should	MD
cord-010188-884d196k	128	11	not	not	RB
cord-010188-884d196k	128	12	only	only	RB
cord-010188-884d196k	128	13	provide	provide	VB
cord-010188-884d196k	128	14	us	-PRON-	PRP
cord-010188-884d196k	128	15	with	with	IN
cord-010188-884d196k	128	16	improved	improved	JJ
cord-010188-884d196k	128	17	expression	expression	NN
cord-010188-884d196k	128	18	systems	system	NNS
cord-010188-884d196k	128	19	,	,	,
cord-010188-884d196k	128	20	but	but	CC
cord-010188-884d196k	128	21	also	also	RB
cord-010188-884d196k	128	22	reveal	reveal	VB
cord-010188-884d196k	128	23	other	other	JJ
cord-010188-884d196k	128	24	interesting	interesting	JJ
cord-010188-884d196k	128	25	facets	facet	NNS
cord-010188-884d196k	128	26	of	of	IN
cord-010188-884d196k	128	27	the	the	DT
cord-010188-884d196k	128	28	biology	biology	NN
cord-010188-884d196k	128	29	of	of	IN
cord-010188-884d196k	128	30	these	these	DT
cord-010188-884d196k	128	31	viruses	virus	NNS
cord-010188-884d196k	128	32	.	.	.
cord-010188-884d196k	129	1	The	the	DT
cord-010188-884d196k	129	2	7bgaviridae	7bgaviridae	CD
cord-010188-884d196k	129	3	and	and	CC
cord-010188-884d196k	129	4	Flaviviridae	Flaviviridae	NNP
cord-010188-884d196k	129	5	Proc	Proc	NNP
cord-010188-884d196k	129	6	.	.	.
cord-010188-884d196k	130	1	Natl	Natl	NNP
cord-010188-884d196k	130	2	Acad	Acad	NNP
cord-010188-884d196k	130	3	.	.	NNP
cord-010188-884d196k	130	4	&	&	CC
cord-010188-884d196k	130	5	i.	i.	NNP
cord-010188-884d196k	130	6	USA	USA	NNP
cord-010188-884d196k	130	7	Proc	Proc	NNP
cord-010188-884d196k	130	8	.	.	.
cord-010188-884d196k	131	1	Natl	Natl	NNP
cord-010188-884d196k	131	2	Acad	Acad	NNP
cord-010188-884d196k	131	3	.	.	NNP
cord-010188-884d196k	131	4	&	&	CC
cord-010188-884d196k	131	5	i.	i.	NNP
cord-010188-884d196k	131	6	USA	USA	NNP
cord-010188-884d196k	131	7	Proc	Proc	NNP
cord-010188-884d196k	131	8	.	.	.
cord-010188-884d196k	132	1	Natl	Natl	NNP
cord-010188-884d196k	132	2	Acad	Acad	NNP
cord-010188-884d196k	132	3	.	.	NNP
cord-010188-884d196k	132	4	&	&	CC
cord-010188-884d196k	132	5	i.	i.	NNP
cord-010188-884d196k	132	6	USA	USA	NNP
cord-010188-884d196k	133	1	The	the	DT
cord-010188-884d196k	133	2	"	"	``
cord-010188-884d196k	133	3	lb2aviridae	lb2aviridae	NNS
cord-010188-884d196k	133	4	and	and	CC
cord-010188-884d196k	133	5	blavivMdae	blavivMdae	.