id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-263239-andje0wu	Dorobantu, Cristina M.	Modulation of the Host Lipid Landscape to Promote RNA Virus Replication: The Picornavirus Encephalomyocarditis Virus Converges on the Pathway Used by Hepatitis C Virus	2015-09-25		.txt	text/plain	8997	455	45	Here we show that cardioviruses manipulate another PI4K, namely the ER-localized phosphatidylinositol 4-kinase III alpha (PI4KA), to generate PI4P-enriched ROs. By siRNA-mediated knockdown and pharmacological inhibition, we demonstrate that PI4KA is an essential host factor for EMCV genome replication. To corroborate that PI4KA activity is required for the step of viral genome replication, we performed a time-of-addition experiment in which AL-9 was added to the cells at different time points after infection with RLuc-EMCV. The Picornavirus EMCV Converges on the Host Lipid Pathway Used by HCV localized throughout the cytoplasm and at the Golgi, OSBP was mainly found at ROs in infected cells, where it largely colocalized with 3AB ( Fig 6D, Pearson's correlation coefficient = 0.71). Finally, data are presented suggesting that the OSBP-mediated exchange of PI4P and cholesterol at RO-MCSs is critical for EMCV genome replication and the global organization of ROs. Membrane alterations in the cytoplasm of cardiovirus-infected cells were already observed decades ago by electron microscopy [37, 38, 63] .	./cache/cord-263239-andje0wu.txt	./txt/cord-263239-andje0wu.txt