id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-331237-t3z1hbox	Ogawa, Hirohito	Molecular epidemiology of pathogenic Leptospira spp. in the straw-colored fruit bat (Eidolon helvum) migrating to Zambia from the Democratic Republic of Congo	2015-03-16		.txt	text/plain	2811	181	57	helvum samples and previously reported sequences, revealed that 12 of the fragments grouped with Leptospira borgpetersenii and Leptospira kirschneri; however, the remaining 58 flaB fragments appeared not to be associated with any reported species. Additionally, the 16S ribosomal RNA gene (rrs) amplified from 27 randomly chosen flaB-positive samples was compared with previously reported sequences, including bat-derived Leptospira spp. A nested PCR based on the flagellin B gene (flaB) sequence was used to amplify the extracted DNA samples (n = 529) to detect the flaB gene of pathogenic Leptospira spp. Six flaB fragments (ZFB08-62, ZFB09-25, ZFB09-32, ZFB12-05, ZFB12-107 and ZFB12-110) in the FC5 cluster were related to the corresponding gene sequences, all of which were identical to Leptospira borgpetersenii strains including Jules, De 10, Arborea, Poi, and Veldrat Batavia 46. The phylogenetic analyses of flaB and rrs infer that genes from potentially pathogenic Leptospira spp.	./cache/cord-331237-t3z1hbox.txt	./txt/cord-331237-t3z1hbox.txt