id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-350762-rh4zbehk	Hutcheson, Jessica M.	Delayed Newcastle disease virus replication using RNA interference to target the nucleoprotein	2015-06-04		.txt	text/plain	4301	229	52	In this study, we utilize microRNA (miRNA)-expressing constructs (a type of RNA interference) in an attempt to target and knockdown five NDV structural RNAs for nucleoprotein (NP), phosphoprotein (P), matrix (M), fusion (F), and large (L) protein genes. Using pre-miRNA to activate the cellular RNAi pathway, a miRNA can be used to target the messenger RNA of NDV structural proteins, leading to the degradation of the transcripts and inhibiting viral replication [11] . In this study, we attempted to determine if constitutive expression of miRNA sequences targeting the mRNA of five of the structural NDV proteins in chicken embryo fibroblast cells (DF-1) would lead to decreased viral yield after infection, and/or resistance against NDV cytopathic effects. Inhibition of Newcastle disease virus replication by RNA interference targeting the matrix protein gene in chicken embryo fibroblasts	./cache/cord-350762-rh4zbehk.txt	./txt/cord-350762-rh4zbehk.txt