id sid tid token lemma pos cord-255350-dmbl4emn 1 1 key key NN cord-255350-dmbl4emn 1 2 : : : cord-255350-dmbl4emn 2 1 cord-255350-dmbl4emn cord-255350-dmbl4emn NNS cord-255350-dmbl4emn 2 2 authors author NNS cord-255350-dmbl4emn 2 3 : : : cord-255350-dmbl4emn 2 4 Bonsor Bonsor NNP cord-255350-dmbl4emn 2 5 , , , cord-255350-dmbl4emn 2 6 Daniel Daniel NNP cord-255350-dmbl4emn 2 7 A. a. NN cord-255350-dmbl4emn 2 8 ; ; : cord-255350-dmbl4emn 2 9 Beckett Beckett NNP cord-255350-dmbl4emn 2 10 , , , cord-255350-dmbl4emn 2 11 Dorothy Dorothy NNP cord-255350-dmbl4emn 2 12 ; ; : cord-255350-dmbl4emn 2 13 Sundberg Sundberg NNP cord-255350-dmbl4emn 2 14 , , , cord-255350-dmbl4emn 2 15 Eric Eric NNP cord-255350-dmbl4emn 2 16 J. J. NNP cord-255350-dmbl4emn 2 17 title title NN cord-255350-dmbl4emn 2 18 : : : cord-255350-dmbl4emn 2 19 Structure structure NN cord-255350-dmbl4emn 2 20 of of IN cord-255350-dmbl4emn 2 21 the the DT cord-255350-dmbl4emn 2 22 N n CD cord-255350-dmbl4emn 2 23 - - HYPH cord-255350-dmbl4emn 2 24 terminal terminal JJ cord-255350-dmbl4emn 2 25 dimerization dimerization NN cord-255350-dmbl4emn 2 26 domain domain NN cord-255350-dmbl4emn 2 27 of of IN cord-255350-dmbl4emn 2 28 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 2 29 date date NN cord-255350-dmbl4emn 2 30 : : : cord-255350-dmbl4emn 2 31 2015 2015 CD cord-255350-dmbl4emn 2 32 - - HYPH cord-255350-dmbl4emn 2 33 08 08 CD cord-255350-dmbl4emn 2 34 - - SYM cord-255350-dmbl4emn 2 35 25 25 CD cord-255350-dmbl4emn 2 36 journal journal NN cord-255350-dmbl4emn 2 37 : : : cord-255350-dmbl4emn 2 38 Acta Acta NNP cord-255350-dmbl4emn 2 39 Crystallographica Crystallographica NNP cord-255350-dmbl4emn 2 40 Section Section NNP cord-255350-dmbl4emn 2 41 F F NNP cord-255350-dmbl4emn 2 42 Structural Structural NNP cord-255350-dmbl4emn 2 43 Biology Biology NNP cord-255350-dmbl4emn 2 44 Communications Communications NNPS cord-255350-dmbl4emn 2 45 DOI DOI NNP cord-255350-dmbl4emn 2 46 : : : cord-255350-dmbl4emn 2 47 10.1107 10.1107 CD cord-255350-dmbl4emn 2 48 / / SYM cord-255350-dmbl4emn 2 49 s2053230x15013576 s2053230x15013576 NN cord-255350-dmbl4emn 2 50 sha sha NNP cord-255350-dmbl4emn 2 51 : : : cord-255350-dmbl4emn 2 52 e8c42853ec4c6de90bb3f9d34077971e6166ced4 e8c42853ec4c6de90bb3f9d34077971e6166ced4 NNP cord-255350-dmbl4emn 2 53 doc_id doc_id NNPS cord-255350-dmbl4emn 2 54 : : : cord-255350-dmbl4emn 2 55 255350 255350 CD cord-255350-dmbl4emn 2 56 cord_uid cord_uid NNS cord-255350-dmbl4emn 2 57 : : : cord-255350-dmbl4emn 2 58 dmbl4emn dmbl4emn NNP cord-255350-dmbl4emn 2 59 CEACAM7 ceacam7 XX cord-255350-dmbl4emn 3 1 is be VBZ cord-255350-dmbl4emn 3 2 a a DT cord-255350-dmbl4emn 3 3 human human JJ cord-255350-dmbl4emn 3 4 cellular cellular JJ cord-255350-dmbl4emn 3 5 adhesion adhesion NN cord-255350-dmbl4emn 3 6 protein protein NN cord-255350-dmbl4emn 3 7 that that WDT cord-255350-dmbl4emn 3 8 is be VBZ cord-255350-dmbl4emn 3 9 expressed express VBN cord-255350-dmbl4emn 3 10 on on IN cord-255350-dmbl4emn 3 11 the the DT cord-255350-dmbl4emn 3 12 surface surface NN cord-255350-dmbl4emn 3 13 of of IN cord-255350-dmbl4emn 3 14 colon colon NN cord-255350-dmbl4emn 3 15 and and CC cord-255350-dmbl4emn 3 16 rectum rectum NN cord-255350-dmbl4emn 3 17 epithelial epithelial JJ cord-255350-dmbl4emn 3 18 cells cell NNS cord-255350-dmbl4emn 3 19 and and CC cord-255350-dmbl4emn 3 20 is be VBZ cord-255350-dmbl4emn 3 21 downregulated downregulate VBN cord-255350-dmbl4emn 3 22 in in IN cord-255350-dmbl4emn 3 23 colorectal colorectal JJ cord-255350-dmbl4emn 3 24 cancers cancer NNS cord-255350-dmbl4emn 3 25 . . . cord-255350-dmbl4emn 4 1 It -PRON- PRP cord-255350-dmbl4emn 4 2 achieves achieve VBZ cord-255350-dmbl4emn 4 3 cell cell NN cord-255350-dmbl4emn 4 4 adhesion adhesion NN cord-255350-dmbl4emn 4 5 through through IN cord-255350-dmbl4emn 4 6 dimerization dimerization NN cord-255350-dmbl4emn 4 7 of of IN cord-255350-dmbl4emn 4 8 the the DT cord-255350-dmbl4emn 4 9 N n CD cord-255350-dmbl4emn 4 10 - - HYPH cord-255350-dmbl4emn 4 11 terminal terminal JJ cord-255350-dmbl4emn 4 12 IgV igv NN cord-255350-dmbl4emn 4 13 domain domain NN cord-255350-dmbl4emn 4 14 . . . cord-255350-dmbl4emn 5 1 The the DT cord-255350-dmbl4emn 5 2 crystal crystal JJ cord-255350-dmbl4emn 5 3 structure structure NN cord-255350-dmbl4emn 5 4 of of IN cord-255350-dmbl4emn 5 5 the the DT cord-255350-dmbl4emn 5 6 N n CD cord-255350-dmbl4emn 5 7 - - HYPH cord-255350-dmbl4emn 5 8 terminal terminal JJ cord-255350-dmbl4emn 5 9 dimerization dimerization NN cord-255350-dmbl4emn 5 10 domain domain NN cord-255350-dmbl4emn 5 11 of of IN cord-255350-dmbl4emn 5 12 CEACAM CEACAM NNP cord-255350-dmbl4emn 5 13 has have VBZ cord-255350-dmbl4emn 5 14 been be VBN cord-255350-dmbl4emn 5 15 determined determine VBN cord-255350-dmbl4emn 5 16 at at IN cord-255350-dmbl4emn 5 17 1.47 1.47 CD cord-255350-dmbl4emn 5 18 Å å NN cord-255350-dmbl4emn 5 19 resolution resolution NN cord-255350-dmbl4emn 5 20 . . . cord-255350-dmbl4emn 6 1 The the DT cord-255350-dmbl4emn 6 2 overall overall JJ cord-255350-dmbl4emn 6 3 fold fold NN cord-255350-dmbl4emn 6 4 of of IN cord-255350-dmbl4emn 6 5 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 6 6 is be VBZ cord-255350-dmbl4emn 6 7 similar similar JJ cord-255350-dmbl4emn 6 8 to to IN cord-255350-dmbl4emn 6 9 those those DT cord-255350-dmbl4emn 6 10 of of IN cord-255350-dmbl4emn 6 11 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 6 12 and and CC cord-255350-dmbl4emn 6 13 CEACAM5 CEACAM5 NNP cord-255350-dmbl4emn 6 14 ; ; : cord-255350-dmbl4emn 6 15 however however RB cord-255350-dmbl4emn 6 16 , , , cord-255350-dmbl4emn 6 17 there there EX cord-255350-dmbl4emn 6 18 are be VBP cord-255350-dmbl4emn 6 19 differences difference NNS cord-255350-dmbl4emn 6 20 , , , cord-255350-dmbl4emn 6 21 the the DT cord-255350-dmbl4emn 6 22 most most RBS cord-255350-dmbl4emn 6 23 notable notable JJ cord-255350-dmbl4emn 6 24 of of IN cord-255350-dmbl4emn 6 25 which which WDT cord-255350-dmbl4emn 6 26 is be VBZ cord-255350-dmbl4emn 6 27 an an DT cord-255350-dmbl4emn 6 28 insertion insertion NN cord-255350-dmbl4emn 6 29 that that WDT cord-255350-dmbl4emn 6 30 causes cause VBZ cord-255350-dmbl4emn 6 31 the the DT cord-255350-dmbl4emn 6 32 C′′ C′′ NNP cord-255350-dmbl4emn 6 33 strand strand NN cord-255350-dmbl4emn 6 34 to to TO cord-255350-dmbl4emn 6 35 buckle buckle NN cord-255350-dmbl4emn 6 36 , , , cord-255350-dmbl4emn 6 37 leading lead VBG cord-255350-dmbl4emn 6 38 to to IN cord-255350-dmbl4emn 6 39 the the DT cord-255350-dmbl4emn 6 40 creation creation NN cord-255350-dmbl4emn 6 41 of of IN cord-255350-dmbl4emn 6 42 a a DT cord-255350-dmbl4emn 6 43 hydrogen hydrogen NN cord-255350-dmbl4emn 6 44 bond bond NN cord-255350-dmbl4emn 6 45 in in IN cord-255350-dmbl4emn 6 46 the the DT cord-255350-dmbl4emn 6 47 dimerization dimerization NN cord-255350-dmbl4emn 6 48 interface interface NN cord-255350-dmbl4emn 6 49 . . . cord-255350-dmbl4emn 7 1 The the DT cord-255350-dmbl4emn 7 2 K K NNP cord-255350-dmbl4emn 7 3 ( ( -LRB- cord-255350-dmbl4emn 7 4 dimerization dimerization NN cord-255350-dmbl4emn 7 5 ) ) -RRB- cord-255350-dmbl4emn 7 6 for for IN cord-255350-dmbl4emn 7 7 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 7 8 determined determine VBN cord-255350-dmbl4emn 7 9 by by IN cord-255350-dmbl4emn 7 10 sedimentation sedimentation NN cord-255350-dmbl4emn 7 11 equilibrium equilibrium NN cord-255350-dmbl4emn 7 12 is be VBZ cord-255350-dmbl4emn 7 13 tenfold tenfold RB cord-255350-dmbl4emn 7 14 tighter tight JJR cord-255350-dmbl4emn 7 15 than than IN cord-255350-dmbl4emn 7 16 that that DT cord-255350-dmbl4emn 7 17 measured measure VBN cord-255350-dmbl4emn 7 18 for for IN cord-255350-dmbl4emn 7 19 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 7 20 . . . cord-255350-dmbl4emn 8 1 These these DT cord-255350-dmbl4emn 8 2 findings finding NNS cord-255350-dmbl4emn 8 3 suggest suggest VBP cord-255350-dmbl4emn 8 4 that that IN cord-255350-dmbl4emn 8 5 the the DT cord-255350-dmbl4emn 8 6 dimerization dimerization NN cord-255350-dmbl4emn 8 7 affinities affinity NNS cord-255350-dmbl4emn 8 8 of of IN cord-255350-dmbl4emn 8 9 CEACAMs ceacam NNS cord-255350-dmbl4emn 8 10 are be VBP cord-255350-dmbl4emn 8 11 modulated modulate VBN cord-255350-dmbl4emn 8 12 via via IN cord-255350-dmbl4emn 8 13 sequence sequence NN cord-255350-dmbl4emn 8 14 variation variation NN cord-255350-dmbl4emn 8 15 in in IN cord-255350-dmbl4emn 8 16 the the DT cord-255350-dmbl4emn 8 17 dimerization dimerization NN cord-255350-dmbl4emn 8 18 surface surface NN cord-255350-dmbl4emn 8 19 . . . cord-255350-dmbl4emn 9 1 Carcinoembryonic Carcinoembryonic NNP cord-255350-dmbl4emn 9 2 antigen antigen NN cord-255350-dmbl4emn 9 3 - - HYPH cord-255350-dmbl4emn 9 4 related relate VBN cord-255350-dmbl4emn 9 5 cell cell NN cord-255350-dmbl4emn 9 6 adhesion adhesion NN cord-255350-dmbl4emn 9 7 molecules molecule NNS cord-255350-dmbl4emn 9 8 ( ( -LRB- cord-255350-dmbl4emn 9 9 CEACAMs ceacam NNS cord-255350-dmbl4emn 9 10 ) ) -RRB- cord-255350-dmbl4emn 9 11 belong belong VBP cord-255350-dmbl4emn 9 12 to to IN cord-255350-dmbl4emn 9 13 the the DT cord-255350-dmbl4emn 9 14 immunoglobulin immunoglobulin NN cord-255350-dmbl4emn 9 15 ( ( -LRB- cord-255350-dmbl4emn 9 16 Ig Ig NNP cord-255350-dmbl4emn 9 17 ) ) -RRB- cord-255350-dmbl4emn 9 18 family family NN cord-255350-dmbl4emn 9 19 and and CC cord-255350-dmbl4emn 9 20 are be VBP cord-255350-dmbl4emn 9 21 expressed express VBN cord-255350-dmbl4emn 9 22 differentially differentially RB cord-255350-dmbl4emn 9 23 on on IN cord-255350-dmbl4emn 9 24 the the DT cord-255350-dmbl4emn 9 25 surfaces surface NNS cord-255350-dmbl4emn 9 26 of of IN cord-255350-dmbl4emn 9 27 cells cell NNS cord-255350-dmbl4emn 9 28 ( ( -LRB- cord-255350-dmbl4emn 9 29 Gray Gray NNP cord-255350-dmbl4emn 9 30 - - HYPH cord-255350-dmbl4emn 9 31 Owen Owen NNP cord-255350-dmbl4emn 9 32 & & CC cord-255350-dmbl4emn 9 33 Blumberg Blumberg NNP cord-255350-dmbl4emn 9 34 , , , cord-255350-dmbl4emn 9 35 2006 2006 CD cord-255350-dmbl4emn 9 36 ; ; : cord-255350-dmbl4emn 9 37 Tchoupa Tchoupa NNP cord-255350-dmbl4emn 9 38 et et NNP cord-255350-dmbl4emn 9 39 al al NNP cord-255350-dmbl4emn 9 40 . . NNP cord-255350-dmbl4emn 9 41 , , , cord-255350-dmbl4emn 9 42 2014 2014 CD cord-255350-dmbl4emn 9 43 ) ) -RRB- cord-255350-dmbl4emn 9 44 . . . cord-255350-dmbl4emn 10 1 There there EX cord-255350-dmbl4emn 10 2 are be VBP cord-255350-dmbl4emn 10 3 12 12 CD cord-255350-dmbl4emn 10 4 CEACAMs ceacam NNS cord-255350-dmbl4emn 10 5 found find VBN cord-255350-dmbl4emn 10 6 in in IN cord-255350-dmbl4emn 10 7 humans human NNS cord-255350-dmbl4emn 10 8 : : : cord-255350-dmbl4emn 10 9 CEACAM1 ceacam1 ADD cord-255350-dmbl4emn 10 10 , , , cord-255350-dmbl4emn 10 11 CEACAM3-CEACAM8 CEACAM3-CEACAM8 NNP cord-255350-dmbl4emn 10 12 , , , cord-255350-dmbl4emn 10 13 CEACAM16 CEACAM16 NNP cord-255350-dmbl4emn 10 14 and and CC cord-255350-dmbl4emn 10 15 CEACAM18-CEACAM21 ceacam18-ceacam21 VB cord-255350-dmbl4emn 11 1 ( ( -LRB- cord-255350-dmbl4emn 11 2 Beauchemin Beauchemin NNP cord-255350-dmbl4emn 11 3 & & CC cord-255350-dmbl4emn 11 4 Arabzadeh Arabzadeh NNP cord-255350-dmbl4emn 11 5 , , , cord-255350-dmbl4emn 11 6 2013 2013 CD cord-255350-dmbl4emn 11 7 ; ; : cord-255350-dmbl4emn 11 8 Tchoupa Tchoupa NNP cord-255350-dmbl4emn 11 9 et et NNP cord-255350-dmbl4emn 11 10 al al NNP cord-255350-dmbl4emn 11 11 . . NNP cord-255350-dmbl4emn 11 12 , , , cord-255350-dmbl4emn 11 13 2014 2014 CD cord-255350-dmbl4emn 11 14 ) ) -RRB- cord-255350-dmbl4emn 11 15 . . . cord-255350-dmbl4emn 12 1 Their -PRON- PRP$ cord-255350-dmbl4emn 12 2 functions function NNS cord-255350-dmbl4emn 12 3 and and CC cord-255350-dmbl4emn 12 4 roles role NNS cord-255350-dmbl4emn 12 5 in in IN cord-255350-dmbl4emn 12 6 cellular cellular JJ cord-255350-dmbl4emn 12 7 processes process NNS cord-255350-dmbl4emn 12 8 are be VBP cord-255350-dmbl4emn 12 9 diverse diverse JJ cord-255350-dmbl4emn 12 10 and and CC cord-255350-dmbl4emn 12 11 include include VBP cord-255350-dmbl4emn 12 12 roles role NNS cord-255350-dmbl4emn 12 13 in in IN cord-255350-dmbl4emn 12 14 phagocytosis phagocytosis NN cord-255350-dmbl4emn 12 15 , , , cord-255350-dmbl4emn 12 16 hearing hearing NN cord-255350-dmbl4emn 12 17 , , , cord-255350-dmbl4emn 12 18 proliferation proliferation NN cord-255350-dmbl4emn 12 19 , , , cord-255350-dmbl4emn 12 20 signaling signal VBG cord-255350-dmbl4emn 12 21 , , , cord-255350-dmbl4emn 12 22 tumor tumor NN cord-255350-dmbl4emn 12 23 suppression suppression NN cord-255350-dmbl4emn 12 24 and and CC cord-255350-dmbl4emn 12 25 cell cell NN cord-255350-dmbl4emn 12 26 adhesion adhesion NN cord-255350-dmbl4emn 12 27 ( ( -LRB- cord-255350-dmbl4emn 12 28 Oikawa Oikawa NNP cord-255350-dmbl4emn 12 29 et et FW cord-255350-dmbl4emn 12 30 al al NNP cord-255350-dmbl4emn 12 31 . . . cord-255350-dmbl4emn 13 1 , , , cord-255350-dmbl4emn 13 2 1989 1989 CD cord-255350-dmbl4emn 13 3 ( ( -LRB- cord-255350-dmbl4emn 13 4 Oikawa Oikawa NNP cord-255350-dmbl4emn 13 5 et et NNP cord-255350-dmbl4emn 13 6 al al NNP cord-255350-dmbl4emn 13 7 . . . cord-255350-dmbl4emn 14 1 , , , cord-255350-dmbl4emn 14 2 , , , cord-255350-dmbl4emn 14 3 1991 1991 CD cord-255350-dmbl4emn 15 1 Benchimol Benchimol NNP cord-255350-dmbl4emn 15 2 et et NNP cord-255350-dmbl4emn 15 3 al al NNP cord-255350-dmbl4emn 15 4 . . NNP cord-255350-dmbl4emn 15 5 , , , cord-255350-dmbl4emn 15 6 1989 1989 CD cord-255350-dmbl4emn 15 7 ; ; : cord-255350-dmbl4emn 16 1 Streichert Streichert NNP cord-255350-dmbl4emn 16 2 et et NNP cord-255350-dmbl4emn 16 3 al al NNP cord-255350-dmbl4emn 16 4 . . NNP cord-255350-dmbl4emn 16 5 , , , cord-255350-dmbl4emn 16 6 2001 2001 CD cord-255350-dmbl4emn 16 7 ; ; : cord-255350-dmbl4emn 17 1 Pils Pils NNP cord-255350-dmbl4emn 17 2 et et NNP cord-255350-dmbl4emn 17 3 al al NNP cord-255350-dmbl4emn 17 4 . . NNP cord-255350-dmbl4emn 17 5 , , , cord-255350-dmbl4emn 17 6 2008 2008 CD cord-255350-dmbl4emn 17 7 ; ; : cord-255350-dmbl4emn 17 8 Singer Singer NNP cord-255350-dmbl4emn 17 9 et et NNP cord-255350-dmbl4emn 17 10 al al NNP cord-255350-dmbl4emn 17 11 . . NNP cord-255350-dmbl4emn 17 12 , , , cord-255350-dmbl4emn 17 13 2010 2010 CD cord-255350-dmbl4emn 17 14 ; ; : cord-255350-dmbl4emn 17 15 Zheng Zheng NNP cord-255350-dmbl4emn 17 16 et et NNP cord-255350-dmbl4emn 17 17 al al NNP cord-255350-dmbl4emn 17 18 . . NNP cord-255350-dmbl4emn 17 19 , , , cord-255350-dmbl4emn 17 20 2011 2011 CD cord-255350-dmbl4emn 17 21 ) ) -RRB- cord-255350-dmbl4emn 17 22 . . . cord-255350-dmbl4emn 18 1 CEACAMs ceacam NNS cord-255350-dmbl4emn 18 2 are be VBP cord-255350-dmbl4emn 18 3 typically typically RB cord-255350-dmbl4emn 18 4 dysregulated dysregulate VBN cord-255350-dmbl4emn 18 5 in in IN cord-255350-dmbl4emn 18 6 cancer cancer NN cord-255350-dmbl4emn 18 7 and and CC cord-255350-dmbl4emn 18 8 are be VBP cord-255350-dmbl4emn 18 9 found find VBN cord-255350-dmbl4emn 18 10 to to TO cord-255350-dmbl4emn 18 11 be be VB cord-255350-dmbl4emn 18 12 parasitized parasitize VBN cord-255350-dmbl4emn 18 13 by by IN cord-255350-dmbl4emn 18 14 bacteria bacteria NNS cord-255350-dmbl4emn 18 15 ( ( -LRB- cord-255350-dmbl4emn 18 16 e.g. e.g. RB cord-255350-dmbl4emn 18 17 Neisseria Neisseria NNP cord-255350-dmbl4emn 18 18 meningitidis meningitidis NN cord-255350-dmbl4emn 18 19 , , , cord-255350-dmbl4emn 18 20 Escherichia Escherichia NNP cord-255350-dmbl4emn 18 21 coli coli NNS cord-255350-dmbl4emn 18 22 and and CC cord-255350-dmbl4emn 18 23 Haemophilus haemophilus JJ cord-255350-dmbl4emn 18 24 influenzae influenzae NN cord-255350-dmbl4emn 18 25 ) ) -RRB- cord-255350-dmbl4emn 18 26 and and CC cord-255350-dmbl4emn 18 27 viruses virus NNS cord-255350-dmbl4emn 18 28 in in IN cord-255350-dmbl4emn 18 29 mice mouse NNS cord-255350-dmbl4emn 18 30 ( ( -LRB- cord-255350-dmbl4emn 18 31 e.g. e.g. RB cord-255350-dmbl4emn 18 32 coronavirus coronavirus NN cord-255350-dmbl4emn 18 33 ) ) -RRB- cord-255350-dmbl4emn 18 34 during during IN cord-255350-dmbl4emn 18 35 infection infection NN cord-255350-dmbl4emn 18 36 ( ( -LRB- cord-255350-dmbl4emn 18 37 Dveksler Dveksler NNP cord-255350-dmbl4emn 18 38 et et FW cord-255350-dmbl4emn 18 39 al al NNP cord-255350-dmbl4emn 18 40 . . NNP cord-255350-dmbl4emn 18 41 , , , cord-255350-dmbl4emn 18 42 1991 1991 CD cord-255350-dmbl4emn 18 43 ; ; : cord-255350-dmbl4emn 18 44 Leusch Leusch NNP cord-255350-dmbl4emn 18 45 et et NNP cord-255350-dmbl4emn 18 46 al al NNP cord-255350-dmbl4emn 18 47 . . . cord-255350-dmbl4emn 19 1 , , , cord-255350-dmbl4emn 19 2 1991 1991 CD cord-255350-dmbl4emn 19 3 ; ; : cord-255350-dmbl4emn 19 4 Bos Bos NNP cord-255350-dmbl4emn 19 5 et et NNP cord-255350-dmbl4emn 19 6 al al NNP cord-255350-dmbl4emn 19 7 . . NNP cord-255350-dmbl4emn 19 8 , , , cord-255350-dmbl4emn 19 9 1997 1997 CD cord-255350-dmbl4emn 19 10 ; ; : cord-255350-dmbl4emn 19 11 Schö Schö NNP cord-255350-dmbl4emn 19 12 lzel lzel NNS cord-255350-dmbl4emn 19 13 et et NNP cord-255350-dmbl4emn 19 14 al al NNP cord-255350-dmbl4emn 19 15 . . NNP cord-255350-dmbl4emn 19 16 , , , cord-255350-dmbl4emn 19 17 2000 2000 CD cord-255350-dmbl4emn 20 1 ; ; : cord-255350-dmbl4emn 20 2 Virji Virji NNP cord-255350-dmbl4emn 20 3 et et NNP cord-255350-dmbl4emn 20 4 al al NNP cord-255350-dmbl4emn 20 5 . . NNP cord-255350-dmbl4emn 20 6 , , , cord-255350-dmbl4emn 20 7 2000 2000 CD cord-255350-dmbl4emn 20 8 ; ; : cord-255350-dmbl4emn 20 9 Duxbury Duxbury NNP cord-255350-dmbl4emn 20 10 et et NNP cord-255350-dmbl4emn 20 11 al al NNP cord-255350-dmbl4emn 20 12 . . NNP cord-255350-dmbl4emn 20 13 , , , cord-255350-dmbl4emn 20 14 2004 2004 CD cord-255350-dmbl4emn 21 1 ; ; : cord-255350-dmbl4emn 21 2 Litkouhi Litkouhi NNP cord-255350-dmbl4emn 21 3 et et NNP cord-255350-dmbl4emn 21 4 al al NNP cord-255350-dmbl4emn 21 5 . . NNP cord-255350-dmbl4emn 21 6 , , , cord-255350-dmbl4emn 21 7 2008 2008 CD cord-255350-dmbl4emn 21 8 ; ; : cord-255350-dmbl4emn 21 9 Obrink Obrink NNP cord-255350-dmbl4emn 21 10 , , , cord-255350-dmbl4emn 21 11 2008 2008 CD cord-255350-dmbl4emn 21 12 ; ; : cord-255350-dmbl4emn 21 13 Singer Singer NNP cord-255350-dmbl4emn 21 14 et et NNP cord-255350-dmbl4emn 21 15 al al NNP cord-255350-dmbl4emn 21 16 . . NNP cord-255350-dmbl4emn 21 17 , , , cord-255350-dmbl4emn 21 18 2010 2010 CD cord-255350-dmbl4emn 21 19 ) ) -RRB- cord-255350-dmbl4emn 21 20 . . . cord-255350-dmbl4emn 22 1 CEACAMs ceacam NNS cord-255350-dmbl4emn 22 2 contain contain VBP cord-255350-dmbl4emn 22 3 an an DT cord-255350-dmbl4emn 22 4 N n CD cord-255350-dmbl4emn 22 5 - - HYPH cord-255350-dmbl4emn 22 6 terminal terminal NN cord-255350-dmbl4emn 22 7 immunoglobulin immunoglobulin NN cord-255350-dmbl4emn 22 8 variable variable NN cord-255350-dmbl4emn 22 9 domain domain NN cord-255350-dmbl4emn 22 10 ( ( -LRB- cord-255350-dmbl4emn 22 11 IgV igv NN cord-255350-dmbl4emn 22 12 ) ) -RRB- cord-255350-dmbl4emn 22 13 , , , cord-255350-dmbl4emn 22 14 a a DT cord-255350-dmbl4emn 22 15 variable variable JJ cord-255350-dmbl4emn 22 16 number number NN cord-255350-dmbl4emn 22 17 of of IN cord-255350-dmbl4emn 22 18 immunoglobulin immunoglobulin NN cord-255350-dmbl4emn 22 19 constant constant JJ cord-255350-dmbl4emn 22 20 domains domain NNS cord-255350-dmbl4emn 22 21 ( ( -LRB- cord-255350-dmbl4emn 22 22 IgC2 igc2 NN cord-255350-dmbl4emn 22 23 ) ) -RRB- cord-255350-dmbl4emn 22 24 and and CC cord-255350-dmbl4emn 22 25 either either CC cord-255350-dmbl4emn 22 26 a a DT cord-255350-dmbl4emn 22 27 C c NN cord-255350-dmbl4emn 22 28 - - HYPH cord-255350-dmbl4emn 22 29 terminal terminal NN cord-255350-dmbl4emn 22 30 transmembrane transmembrane NN cord-255350-dmbl4emn 22 31 and and CC cord-255350-dmbl4emn 22 32 cytoplasmic cytoplasmic JJ cord-255350-dmbl4emn 22 33 domain domain NN cord-255350-dmbl4emn 22 34 or or CC cord-255350-dmbl4emn 22 35 a a DT cord-255350-dmbl4emn 22 36 glycophosphatidyl glycophosphatidyl NN cord-255350-dmbl4emn 22 37 - - HYPH cord-255350-dmbl4emn 22 38 inositol inositol JJ cord-255350-dmbl4emn 22 39 ( ( -LRB- cord-255350-dmbl4emn 22 40 GPI GPI NNP cord-255350-dmbl4emn 22 41 ) ) -RRB- cord-255350-dmbl4emn 22 42 moiety moiety NN cord-255350-dmbl4emn 22 43 by by IN cord-255350-dmbl4emn 22 44 which which WDT cord-255350-dmbl4emn 22 45 they -PRON- PRP cord-255350-dmbl4emn 22 46 are be VBP cord-255350-dmbl4emn 22 47 anchored anchor VBN cord-255350-dmbl4emn 22 48 to to IN cord-255350-dmbl4emn 22 49 the the DT cord-255350-dmbl4emn 22 50 plasma plasma NN cord-255350-dmbl4emn 22 51 membrane membrane NN cord-255350-dmbl4emn 22 52 ( ( -LRB- cord-255350-dmbl4emn 22 53 Tchoupa Tchoupa NNP cord-255350-dmbl4emn 22 54 et et FW cord-255350-dmbl4emn 22 55 al al NNP cord-255350-dmbl4emn 22 56 . . NNP cord-255350-dmbl4emn 22 57 , , , cord-255350-dmbl4emn 22 58 2014 2014 CD cord-255350-dmbl4emn 22 59 ) ) -RRB- cord-255350-dmbl4emn 22 60 . . . cord-255350-dmbl4emn 23 1 Cell cell NN cord-255350-dmbl4emn 23 2 adhesion adhesion NN cord-255350-dmbl4emn 23 3 is be VBZ cord-255350-dmbl4emn 23 4 achieved achieve VBN cord-255350-dmbl4emn 23 5 through through IN cord-255350-dmbl4emn 23 6 the the DT cord-255350-dmbl4emn 23 7 N n CD cord-255350-dmbl4emn 23 8 - - HYPH cord-255350-dmbl4emn 23 9 terminal terminal NN cord-255350-dmbl4emn 23 10 domain domain NN cord-255350-dmbl4emn 23 11 of of IN cord-255350-dmbl4emn 23 12 CEACAMs ceacam NNS cord-255350-dmbl4emn 23 13 , , , cord-255350-dmbl4emn 23 14 which which WDT cord-255350-dmbl4emn 23 15 can can MD cord-255350-dmbl4emn 23 16 undergo undergo VB cord-255350-dmbl4emn 23 17 heterodimerization heterodimerization NN cord-255350-dmbl4emn 23 18 and and CC cord-255350-dmbl4emn 23 19 homodimerization homodimerization NN cord-255350-dmbl4emn 23 20 in in IN cord-255350-dmbl4emn 23 21 a a DT cord-255350-dmbl4emn 23 22 cis cis NNP cord-255350-dmbl4emn 23 23 ( ( -LRB- cord-255350-dmbl4emn 23 24 on on IN cord-255350-dmbl4emn 23 25 the the DT cord-255350-dmbl4emn 23 26 same same JJ cord-255350-dmbl4emn 23 27 cell cell NN cord-255350-dmbl4emn 23 28 ) ) -RRB- cord-255350-dmbl4emn 23 29 or or CC cord-255350-dmbl4emn 23 30 trans trans NNP cord-255350-dmbl4emn 23 31 ( ( -LRB- cord-255350-dmbl4emn 23 32 across across IN cord-255350-dmbl4emn 23 33 different different JJ cord-255350-dmbl4emn 23 34 cells cell NNS cord-255350-dmbl4emn 23 35 ) ) -RRB- cord-255350-dmbl4emn 23 36 fashion fashion NN cord-255350-dmbl4emn 24 1 ( ( -LRB- cord-255350-dmbl4emn 24 2 Taheri Taheri NNP cord-255350-dmbl4emn 24 3 et et FW cord-255350-dmbl4emn 24 4 al al NNP cord-255350-dmbl4emn 24 5 . . NNP cord-255350-dmbl4emn 24 6 , , , cord-255350-dmbl4emn 24 7 2000 2000 CD cord-255350-dmbl4emn 24 8 ; ; : cord-255350-dmbl4emn 25 1 Watt Watt NNP cord-255350-dmbl4emn 25 2 et et NNP cord-255350-dmbl4emn 25 3 al al NNP cord-255350-dmbl4emn 25 4 . . NNP cord-255350-dmbl4emn 25 5 , , , cord-255350-dmbl4emn 25 6 2001 2001 CD cord-255350-dmbl4emn 25 7 ) ) -RRB- cord-255350-dmbl4emn 25 8 . . . cord-255350-dmbl4emn 26 1 # # $ cord-255350-dmbl4emn 26 2 2015 2015 CD cord-255350-dmbl4emn 26 3 International International NNP cord-255350-dmbl4emn 26 4 Union Union NNP cord-255350-dmbl4emn 26 5 of of IN cord-255350-dmbl4emn 26 6 Crystallography Crystallography NNP cord-255350-dmbl4emn 26 7 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 26 8 is be VBZ cord-255350-dmbl4emn 26 9 expressed express VBN cord-255350-dmbl4emn 26 10 on on IN cord-255350-dmbl4emn 26 11 highly highly RB cord-255350-dmbl4emn 26 12 differentiated differentiated JJ cord-255350-dmbl4emn 26 13 epithelial epithelial JJ cord-255350-dmbl4emn 26 14 cells cell NNS cord-255350-dmbl4emn 26 15 of of IN cord-255350-dmbl4emn 26 16 the the DT cord-255350-dmbl4emn 26 17 colon colon NN cord-255350-dmbl4emn 26 18 and and CC cord-255350-dmbl4emn 26 19 rectum rectum NN cord-255350-dmbl4emn 26 20 and and CC cord-255350-dmbl4emn 26 21 on on IN cord-255350-dmbl4emn 26 22 the the DT cord-255350-dmbl4emn 26 23 epithelial epithelial JJ cord-255350-dmbl4emn 26 24 cells cell NNS cord-255350-dmbl4emn 26 25 within within IN cord-255350-dmbl4emn 26 26 the the DT cord-255350-dmbl4emn 26 27 ducts duct NNS cord-255350-dmbl4emn 26 28 of of IN cord-255350-dmbl4emn 26 29 the the DT cord-255350-dmbl4emn 26 30 pancreas pancrea NNS cord-255350-dmbl4emn 26 31 ( ( -LRB- cord-255350-dmbl4emn 26 32 Schö Schö NNP cord-255350-dmbl4emn 26 33 lzel lzel NNS cord-255350-dmbl4emn 26 34 et et NNP cord-255350-dmbl4emn 26 35 al al NNP cord-255350-dmbl4emn 26 36 . . NNP cord-255350-dmbl4emn 26 37 , , , cord-255350-dmbl4emn 26 38 2000 2000 CD cord-255350-dmbl4emn 26 39 ) ) -RRB- cord-255350-dmbl4emn 26 40 . . . cord-255350-dmbl4emn 27 1 The the DT cord-255350-dmbl4emn 27 2 expression expression NN cord-255350-dmbl4emn 27 3 pattern pattern NN cord-255350-dmbl4emn 27 4 of of IN cord-255350-dmbl4emn 27 5 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 27 6 suggests suggest VBZ cord-255350-dmbl4emn 27 7 a a DT cord-255350-dmbl4emn 27 8 specialized specialized JJ cord-255350-dmbl4emn 27 9 function function NN cord-255350-dmbl4emn 27 10 . . . cord-255350-dmbl4emn 28 1 In in IN cord-255350-dmbl4emn 28 2 fetal fetal JJ cord-255350-dmbl4emn 28 3 tissues tissue NNS cord-255350-dmbl4emn 28 4 of of IN cord-255350-dmbl4emn 28 5 the the DT cord-255350-dmbl4emn 28 6 colon colon NN cord-255350-dmbl4emn 28 7 , , , cord-255350-dmbl4emn 28 8 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 28 9 is be VBZ cord-255350-dmbl4emn 28 10 located locate VBN cord-255350-dmbl4emn 28 11 at at IN cord-255350-dmbl4emn 28 12 the the DT cord-255350-dmbl4emn 28 13 base base NN cord-255350-dmbl4emn 28 14 of of IN cord-255350-dmbl4emn 28 15 epithelial epithelial JJ cord-255350-dmbl4emn 28 16 cells cell NNS cord-255350-dmbl4emn 28 17 and and CC cord-255350-dmbl4emn 28 18 has have VBZ cord-255350-dmbl4emn 28 19 been be VBN cord-255350-dmbl4emn 28 20 found find VBN cord-255350-dmbl4emn 28 21 to to TO cord-255350-dmbl4emn 28 22 migrate migrate VB cord-255350-dmbl4emn 28 23 to to IN cord-255350-dmbl4emn 28 24 the the DT cord-255350-dmbl4emn 28 25 apical apical JJ cord-255350-dmbl4emn 28 26 surface surface NN cord-255350-dmbl4emn 28 27 a a DT cord-255350-dmbl4emn 28 28 few few JJ cord-255350-dmbl4emn 28 29 days day NNS cord-255350-dmbl4emn 28 30 after after IN cord-255350-dmbl4emn 28 31 birth birth NN cord-255350-dmbl4emn 29 1 ( ( -LRB- cord-255350-dmbl4emn 29 2 Schö Schö NNP cord-255350-dmbl4emn 29 3 lzel lzel NNS cord-255350-dmbl4emn 29 4 et et NNP cord-255350-dmbl4emn 29 5 al al NNP cord-255350-dmbl4emn 29 6 . . . cord-255350-dmbl4emn 30 1 , , , cord-255350-dmbl4emn 30 2 2000 2000 CD cord-255350-dmbl4emn 30 3 ) ) -RRB- cord-255350-dmbl4emn 30 4 . . . cord-255350-dmbl4emn 31 1 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 31 2 contains contain VBZ cord-255350-dmbl4emn 31 3 three three CD cord-255350-dmbl4emn 31 4 domains domain NNS cord-255350-dmbl4emn 31 5 : : : cord-255350-dmbl4emn 31 6 an an DT cord-255350-dmbl4emn 31 7 N n CD cord-255350-dmbl4emn 31 8 - - HYPH cord-255350-dmbl4emn 31 9 terminal terminal JJ cord-255350-dmbl4emn 31 10 IgV igv NN cord-255350-dmbl4emn 31 11 domain domain NN cord-255350-dmbl4emn 31 12 , , , cord-255350-dmbl4emn 31 13 a a DT cord-255350-dmbl4emn 31 14 single single JJ cord-255350-dmbl4emn 31 15 IgC2 igc2 JJ cord-255350-dmbl4emn 31 16 domain domain NN cord-255350-dmbl4emn 31 17 and and CC cord-255350-dmbl4emn 31 18 a a DT cord-255350-dmbl4emn 31 19 cell cell NN cord-255350-dmbl4emn 31 20 - - HYPH cord-255350-dmbl4emn 31 21 surface surface NN cord-255350-dmbl4emn 31 22 GPI GPI NNP cord-255350-dmbl4emn 31 23 anchor anchor NN cord-255350-dmbl4emn 31 24 domain domain NN cord-255350-dmbl4emn 31 25 ( ( -LRB- cord-255350-dmbl4emn 31 26 Tchoupa Tchoupa NNP cord-255350-dmbl4emn 31 27 et et FW cord-255350-dmbl4emn 31 28 al al NNP cord-255350-dmbl4emn 31 29 . . NNP cord-255350-dmbl4emn 31 30 , , , cord-255350-dmbl4emn 31 31 2014 2014 CD cord-255350-dmbl4emn 31 32 ) ) -RRB- cord-255350-dmbl4emn 31 33 . . . cord-255350-dmbl4emn 32 1 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 32 2 expression expression NN cord-255350-dmbl4emn 32 3 is be VBZ cord-255350-dmbl4emn 32 4 downregulated downregulate VBN cord-255350-dmbl4emn 32 5 during during IN cord-255350-dmbl4emn 32 6 the the DT cord-255350-dmbl4emn 32 7 early early JJ cord-255350-dmbl4emn 32 8 development development NN cord-255350-dmbl4emn 32 9 of of IN cord-255350-dmbl4emn 32 10 colorectal colorectal JJ cord-255350-dmbl4emn 32 11 tumors tumor NNS cord-255350-dmbl4emn 32 12 , , , cord-255350-dmbl4emn 32 13 unlike unlike IN cord-255350-dmbl4emn 32 14 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 32 15 or or CC cord-255350-dmbl4emn 32 16 CEACAM6 ceacam6 NN cord-255350-dmbl4emn 32 17 , , , cord-255350-dmbl4emn 32 18 which which WDT cord-255350-dmbl4emn 32 19 are be VBP cord-255350-dmbl4emn 32 20 typically typically RB cord-255350-dmbl4emn 32 21 upregulated upregulate VBN cord-255350-dmbl4emn 32 22 , , , cord-255350-dmbl4emn 32 23 suggesting suggest VBG cord-255350-dmbl4emn 32 24 a a DT cord-255350-dmbl4emn 32 25 tumor tumor NN cord-255350-dmbl4emn 32 26 - - HYPH cord-255350-dmbl4emn 32 27 suppression suppression NN cord-255350-dmbl4emn 32 28 function function NN cord-255350-dmbl4emn 32 29 ( ( -LRB- cord-255350-dmbl4emn 32 30 Schö Schö NNP cord-255350-dmbl4emn 32 31 lzel lzel NNS cord-255350-dmbl4emn 32 32 et et NNP cord-255350-dmbl4emn 32 33 al al NNP cord-255350-dmbl4emn 32 34 . . NNP cord-255350-dmbl4emn 32 35 , , , cord-255350-dmbl4emn 32 36 2000 2000 CD cord-255350-dmbl4emn 32 37 ) ) -RRB- cord-255350-dmbl4emn 32 38 . . . cord-255350-dmbl4emn 33 1 Currently currently RB cord-255350-dmbl4emn 33 2 , , , cord-255350-dmbl4emn 33 3 it -PRON- PRP cord-255350-dmbl4emn 33 4 is be VBZ cord-255350-dmbl4emn 33 5 unknown unknown JJ cord-255350-dmbl4emn 33 6 whether whether IN cord-255350-dmbl4emn 33 7 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 33 8 is be VBZ cord-255350-dmbl4emn 33 9 involved involve VBN cord-255350-dmbl4emn 33 10 in in IN cord-255350-dmbl4emn 33 11 cell cell NN cord-255350-dmbl4emn 33 12 adhesion adhesion NN cord-255350-dmbl4emn 33 13 through through IN cord-255350-dmbl4emn 33 14 homodimerization homodimerization NN cord-255350-dmbl4emn 33 15 or or CC cord-255350-dmbl4emn 33 16 if if IN cord-255350-dmbl4emn 33 17 any any DT cord-255350-dmbl4emn 33 18 structural structural JJ cord-255350-dmbl4emn 33 19 differences difference NNS cord-255350-dmbl4emn 33 20 exist exist VBP cord-255350-dmbl4emn 33 21 that that WDT cord-255350-dmbl4emn 33 22 could could MD cord-255350-dmbl4emn 33 23 potentially potentially RB cord-255350-dmbl4emn 33 24 allow allow VB cord-255350-dmbl4emn 33 25 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 33 26 to to TO cord-255350-dmbl4emn 33 27 function function VB cord-255350-dmbl4emn 33 28 as as IN cord-255350-dmbl4emn 33 29 a a DT cord-255350-dmbl4emn 33 30 tumorsuppression tumorsuppression NN cord-255350-dmbl4emn 33 31 molecule molecule NN cord-255350-dmbl4emn 33 32 when when WRB cord-255350-dmbl4emn 33 33 compared compare VBN cord-255350-dmbl4emn 33 34 with with IN cord-255350-dmbl4emn 33 35 the the DT cord-255350-dmbl4emn 33 36 two two CD cord-255350-dmbl4emn 33 37 known know VBN cord-255350-dmbl4emn 33 38 structures structure NNS cord-255350-dmbl4emn 33 39 of of IN cord-255350-dmbl4emn 33 40 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 33 41 and and CC cord-255350-dmbl4emn 33 42 CEACAM5 CEACAM5 NNP cord-255350-dmbl4emn 34 1 ( ( -LRB- cord-255350-dmbl4emn 34 2 Fedarovich Fedarovich NNP cord-255350-dmbl4emn 34 3 et et NNP cord-255350-dmbl4emn 34 4 al al NNP cord-255350-dmbl4emn 34 5 . . NNP cord-255350-dmbl4emn 34 6 , , , cord-255350-dmbl4emn 34 7 2006 2006 CD cord-255350-dmbl4emn 34 8 ; ; : cord-255350-dmbl4emn 34 9 Korotkova Korotkova NNP cord-255350-dmbl4emn 34 10 et et FW cord-255350-dmbl4emn 34 11 al al NNP cord-255350-dmbl4emn 34 12 . . NNP cord-255350-dmbl4emn 34 13 , , , cord-255350-dmbl4emn 34 14 2008 2008 CD cord-255350-dmbl4emn 34 15 ) ) -RRB- cord-255350-dmbl4emn 34 16 . . . cord-255350-dmbl4emn 35 1 Here here RB cord-255350-dmbl4emn 35 2 , , , cord-255350-dmbl4emn 35 3 we -PRON- PRP cord-255350-dmbl4emn 35 4 report report VBP cord-255350-dmbl4emn 35 5 the the DT cord-255350-dmbl4emn 35 6 1.47 1.47 CD cord-255350-dmbl4emn 35 7 Å å NN cord-255350-dmbl4emn 35 8 resolution resolution NN cord-255350-dmbl4emn 35 9 X x NN cord-255350-dmbl4emn 35 10 - - NN cord-255350-dmbl4emn 35 11 ray ray NN cord-255350-dmbl4emn 35 12 crystal crystal NN cord-255350-dmbl4emn 35 13 structure structure NN cord-255350-dmbl4emn 35 14 of of IN cord-255350-dmbl4emn 35 15 the the DT cord-255350-dmbl4emn 35 16 N n CD cord-255350-dmbl4emn 35 17 - - HYPH cord-255350-dmbl4emn 35 18 terminal terminal NN cord-255350-dmbl4emn 35 19 domain domain NN cord-255350-dmbl4emn 35 20 of of IN cord-255350-dmbl4emn 35 21 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 35 22 and and CC cord-255350-dmbl4emn 35 23 have have VBP cord-255350-dmbl4emn 35 24 characterized characterize VBN cord-255350-dmbl4emn 35 25 its -PRON- PRP$ cord-255350-dmbl4emn 35 26 oligomeric oligomeric JJ cord-255350-dmbl4emn 35 27 state state NN cord-255350-dmbl4emn 35 28 in in IN cord-255350-dmbl4emn 35 29 solution solution NN cord-255350-dmbl4emn 35 30 . . . cord-255350-dmbl4emn 36 1 The the DT cord-255350-dmbl4emn 36 2 N n CD cord-255350-dmbl4emn 36 3 - - HYPH cord-255350-dmbl4emn 36 4 terminal terminal NN cord-255350-dmbl4emn 36 5 domain domain NN cord-255350-dmbl4emn 36 6 of of IN cord-255350-dmbl4emn 36 7 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 36 8 was be VBD cord-255350-dmbl4emn 36 9 synthesized synthesize VBN cord-255350-dmbl4emn 36 10 as as IN cord-255350-dmbl4emn 36 11 a a DT cord-255350-dmbl4emn 36 12 codon codon NN cord-255350-dmbl4emn 36 13 - - HYPH cord-255350-dmbl4emn 36 14 optimized optimize VBN cord-255350-dmbl4emn 36 15 GeneArt GeneArt NNP cord-255350-dmbl4emn 36 16 string string NN cord-255350-dmbl4emn 36 17 ( ( -LRB- cord-255350-dmbl4emn 36 18 Life Life NNP cord-255350-dmbl4emn 36 19 Technologies Technologies NNPS cord-255350-dmbl4emn 36 20 ) ) -RRB- cord-255350-dmbl4emn 36 21 , , , cord-255350-dmbl4emn 36 22 which which WDT cord-255350-dmbl4emn 36 23 was be VBD cord-255350-dmbl4emn 36 24 digested digest VBN cord-255350-dmbl4emn 36 25 and and CC cord-255350-dmbl4emn 36 26 ligated ligate VBN cord-255350-dmbl4emn 36 27 into into IN cord-255350-dmbl4emn 36 28 an an DT cord-255350-dmbl4emn 36 29 NcoI NcoI NNP cord-255350-dmbl4emn 36 30 / / SYM cord-255350-dmbl4emn 36 31 XhoI XhoI NNP cord-255350-dmbl4emn 36 32 - - HYPH cord-255350-dmbl4emn 36 33 cut cut VBN cord-255350-dmbl4emn 36 34 pET-21d pet-21d JJ cord-255350-dmbl4emn 36 35 vector vector NN cord-255350-dmbl4emn 36 36 without without IN cord-255350-dmbl4emn 36 37 a a DT cord-255350-dmbl4emn 36 38 purification purification NN cord-255350-dmbl4emn 36 39 tag tag NN cord-255350-dmbl4emn 36 40 . . . cord-255350-dmbl4emn 37 1 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 37 2 was be VBD cord-255350-dmbl4emn 37 3 expressed express VBN cord-255350-dmbl4emn 37 4 in in IN cord-255350-dmbl4emn 37 5 inclusion inclusion NN cord-255350-dmbl4emn 37 6 bodies body NNS cord-255350-dmbl4emn 37 7 in in IN cord-255350-dmbl4emn 37 8 E. E. NNP cord-255350-dmbl4emn 37 9 coli coli NNS cord-255350-dmbl4emn 38 1 BL21 BL21 NNP cord-255350-dmbl4emn 38 2 ( ( -LRB- cord-255350-dmbl4emn 39 1 DE3 DE3 NNP cord-255350-dmbl4emn 39 2 ) ) -RRB- cord-255350-dmbl4emn 39 3 pLysS pLysS NNP cord-255350-dmbl4emn 39 4 cells cell NNS cord-255350-dmbl4emn 39 5 . . . cord-255350-dmbl4emn 40 1 Briefly briefly RB cord-255350-dmbl4emn 40 2 , , , cord-255350-dmbl4emn 40 3 1 1 CD cord-255350-dmbl4emn 40 4 l l NN cord-255350-dmbl4emn 40 5 of of IN cord-255350-dmbl4emn 40 6 cells cell NNS cord-255350-dmbl4emn 40 7 were be VBD cord-255350-dmbl4emn 40 8 grown grow VBN cord-255350-dmbl4emn 40 9 in in IN cord-255350-dmbl4emn 40 10 LB LB NNP cord-255350-dmbl4emn 40 11 Miller Miller NNP cord-255350-dmbl4emn 40 12 at at IN cord-255350-dmbl4emn 40 13 310 310 CD cord-255350-dmbl4emn 40 14 K k NN cord-255350-dmbl4emn 40 15 until until IN cord-255350-dmbl4emn 40 16 an an DT cord-255350-dmbl4emn 40 17 OD OD NNP cord-255350-dmbl4emn 40 18 600 600 CD cord-255350-dmbl4emn 40 19 nm nm NN cord-255350-dmbl4emn 40 20 of of IN cord-255350-dmbl4emn 40 21 $ $ $ cord-255350-dmbl4emn 40 22 0.6 0.6 CD cord-255350-dmbl4emn 40 23 was be VBD cord-255350-dmbl4emn 40 24 attained attain VBN cord-255350-dmbl4emn 40 25 , , , cord-255350-dmbl4emn 40 26 prior prior RB cord-255350-dmbl4emn 40 27 to to IN cord-255350-dmbl4emn 40 28 induction induction NN cord-255350-dmbl4emn 40 29 with with IN cord-255350-dmbl4emn 40 30 1 1 CD cord-255350-dmbl4emn 40 31 mM mm CD cord-255350-dmbl4emn 40 32 isopropyl isopropyl NN cord-255350-dmbl4emn 40 33 -d-1-thiogalactopyranoside -d-1-thiogalactopyranoside . cord-255350-dmbl4emn 41 1 ( ( -LRB- cord-255350-dmbl4emn 41 2 IPTG IPTG NNP cord-255350-dmbl4emn 41 3 ) ) -RRB- cord-255350-dmbl4emn 41 4 . . . cord-255350-dmbl4emn 42 1 Cells cell NNS cord-255350-dmbl4emn 42 2 were be VBD cord-255350-dmbl4emn 42 3 grown grow VBN cord-255350-dmbl4emn 42 4 for for IN cord-255350-dmbl4emn 42 5 a a DT cord-255350-dmbl4emn 42 6 further further JJ cord-255350-dmbl4emn 42 7 4 4 CD cord-255350-dmbl4emn 42 8 h h NN cord-255350-dmbl4emn 42 9 before before IN cord-255350-dmbl4emn 42 10 harvesting harvesting NN cord-255350-dmbl4emn 42 11 ( ( -LRB- cord-255350-dmbl4emn 42 12 5000 5000 CD cord-255350-dmbl4emn 42 13 g g NN cord-255350-dmbl4emn 42 14 for for IN cord-255350-dmbl4emn 42 15 15 15 CD cord-255350-dmbl4emn 42 16 min min NN cord-255350-dmbl4emn 42 17 at at IN cord-255350-dmbl4emn 42 18 277 277 CD cord-255350-dmbl4emn 42 19 K K NNP cord-255350-dmbl4emn 42 20 ) ) -RRB- cord-255350-dmbl4emn 42 21 . . . cord-255350-dmbl4emn 43 1 The the DT cord-255350-dmbl4emn 43 2 cells cell NNS cord-255350-dmbl4emn 43 3 were be VBD cord-255350-dmbl4emn 43 4 resuspended resuspend VBN cord-255350-dmbl4emn 43 5 in in IN cord-255350-dmbl4emn 43 6 lysis lysis NN cord-255350-dmbl4emn 43 7 buffer buffer NN cord-255350-dmbl4emn 43 8 [ [ -LRB- cord-255350-dmbl4emn 43 9 50 50 CD cord-255350-dmbl4emn 43 10 mM mm CD cord-255350-dmbl4emn 43 11 Tris Tris NNP cord-255350-dmbl4emn 43 12 - - HYPH cord-255350-dmbl4emn 43 13 HCl HCl NNP cord-255350-dmbl4emn 43 14 , , , cord-255350-dmbl4emn 43 15 500 500 CD cord-255350-dmbl4emn 43 16 mM mm CD cord-255350-dmbl4emn 43 17 NaCl nacl NN cord-255350-dmbl4emn 43 18 , , , cord-255350-dmbl4emn 43 19 1%(v 1%(v CD cord-255350-dmbl4emn 43 20 / / SYM cord-255350-dmbl4emn 43 21 v v NN cord-255350-dmbl4emn 43 22 ) ) -RRB- cord-255350-dmbl4emn 44 1 Triton Triton NNP cord-255350-dmbl4emn 44 2 X-100 X-100 VBD cord-255350-dmbl4emn 44 3 pH pH NNP cord-255350-dmbl4emn 44 4 7.5 7.5 CD cord-255350-dmbl4emn 44 5 ] ] -RRB- cord-255350-dmbl4emn 44 6 and and CC cord-255350-dmbl4emn 44 7 lysed lyse VBN cord-255350-dmbl4emn 44 8 by by IN cord-255350-dmbl4emn 44 9 sonication sonication NN cord-255350-dmbl4emn 44 10 . . . cord-255350-dmbl4emn 45 1 Inclusion inclusion NN cord-255350-dmbl4emn 45 2 bodies body NNS cord-255350-dmbl4emn 45 3 were be VBD cord-255350-dmbl4emn 45 4 isolated isolate VBN cord-255350-dmbl4emn 45 5 ( ( -LRB- cord-255350-dmbl4emn 45 6 20 20 CD cord-255350-dmbl4emn 45 7 000 000 CD cord-255350-dmbl4emn 45 8 g g NN cord-255350-dmbl4emn 45 9 for for IN cord-255350-dmbl4emn 45 10 20 20 CD cord-255350-dmbl4emn 45 11 min min NN cord-255350-dmbl4emn 45 12 at at IN cord-255350-dmbl4emn 45 13 277 277 CD cord-255350-dmbl4emn 45 14 K K NNP cord-255350-dmbl4emn 45 15 ) ) -RRB- cord-255350-dmbl4emn 45 16 , , , cord-255350-dmbl4emn 45 17 resuspended resuspend VBN cord-255350-dmbl4emn 45 18 in in IN cord-255350-dmbl4emn 45 19 lysis lysis NN cord-255350-dmbl4emn 45 20 buffer buffer NN cord-255350-dmbl4emn 45 21 , , , cord-255350-dmbl4emn 45 22 sonicated sonicate VBN cord-255350-dmbl4emn 45 23 and and CC cord-255350-dmbl4emn 45 24 isolated isolate VBN cord-255350-dmbl4emn 45 25 by by IN cord-255350-dmbl4emn 45 26 centrifugation centrifugation NN cord-255350-dmbl4emn 45 27 ( ( -LRB- cord-255350-dmbl4emn 45 28 20 20 CD cord-255350-dmbl4emn 45 29 000 000 CD cord-255350-dmbl4emn 45 30 g g NN cord-255350-dmbl4emn 45 31 for for IN cord-255350-dmbl4emn 45 32 20 20 CD cord-255350-dmbl4emn 45 33 min min NN cord-255350-dmbl4emn 45 34 at at IN cord-255350-dmbl4emn 45 35 277 277 CD cord-255350-dmbl4emn 45 36 K K NNP cord-255350-dmbl4emn 45 37 ) ) -RRB- cord-255350-dmbl4emn 45 38 . . . cord-255350-dmbl4emn 46 1 Inclusion inclusion NN cord-255350-dmbl4emn 46 2 bodies body NNS cord-255350-dmbl4emn 46 3 were be VBD cord-255350-dmbl4emn 46 4 washed wash VBN cord-255350-dmbl4emn 46 5 with with IN cord-255350-dmbl4emn 46 6 a a DT cord-255350-dmbl4emn 46 7 high high JJ cord-255350-dmbl4emn 46 8 - - HYPH cord-255350-dmbl4emn 46 9 salt salt NN cord-255350-dmbl4emn 46 10 buffer buffer NN cord-255350-dmbl4emn 46 11 ( ( -LRB- cord-255350-dmbl4emn 46 12 50 50 CD cord-255350-dmbl4emn 46 13 mM mM NNP cord-255350-dmbl4emn 46 14 Tris Tris NNP cord-255350-dmbl4emn 46 15 - - HYPH cord-255350-dmbl4emn 46 16 HCl HCl NNP cord-255350-dmbl4emn 46 17 , , , cord-255350-dmbl4emn 46 18 1.0 1.0 CD cord-255350-dmbl4emn 46 19 M m NN cord-255350-dmbl4emn 46 20 NaCl nacl NN cord-255350-dmbl4emn 46 21 pH pH NNP cord-255350-dmbl4emn 46 22 8.0 8.0 CD cord-255350-dmbl4emn 46 23 ) ) -RRB- cord-255350-dmbl4emn 46 24 to to TO cord-255350-dmbl4emn 46 25 remove remove VB cord-255350-dmbl4emn 46 26 DNA DNA NNP cord-255350-dmbl4emn 46 27 , , , cord-255350-dmbl4emn 46 28 followed follow VBN cord-255350-dmbl4emn 46 29 by by IN cord-255350-dmbl4emn 46 30 lysis lysis NN cord-255350-dmbl4emn 46 31 buffer buffer NN cord-255350-dmbl4emn 46 32 without without IN cord-255350-dmbl4emn 46 33 Triton Triton NNP cord-255350-dmbl4emn 46 34 X-100 x-100 RB cord-255350-dmbl4emn 46 35 . . . cord-255350-dmbl4emn 47 1 Inclusion inclusion NN cord-255350-dmbl4emn 47 2 bodies body NNS cord-255350-dmbl4emn 47 3 were be VBD cord-255350-dmbl4emn 47 4 dissolved dissolve VBN cord-255350-dmbl4emn 47 5 in in IN cord-255350-dmbl4emn 47 6 30 30 CD cord-255350-dmbl4emn 47 7 mM mm CD cord-255350-dmbl4emn 47 8 Tris Tris NNP cord-255350-dmbl4emn 47 9 - - HYPH cord-255350-dmbl4emn 47 10 HCl HCl NNP cord-255350-dmbl4emn 47 11 , , , cord-255350-dmbl4emn 47 12 150 150 CD cord-255350-dmbl4emn 47 13 mM mm CD cord-255350-dmbl4emn 47 14 NaCl nacl NN cord-255350-dmbl4emn 47 15 , , , cord-255350-dmbl4emn 47 16 8.0 8.0 CD cord-255350-dmbl4emn 47 17 M m NN cord-255350-dmbl4emn 47 18 urea urea NN cord-255350-dmbl4emn 47 19 pH pH NNP cord-255350-dmbl4emn 47 20 8.3 8.3 CD cord-255350-dmbl4emn 47 21 ( ( -LRB- cord-255350-dmbl4emn 47 22 $ $ $ cord-255350-dmbl4emn 47 23 5 5 CD cord-255350-dmbl4emn 47 24 ml ml NNS cord-255350-dmbl4emn 47 25 per per IN cord-255350-dmbl4emn 47 26 litre litre NN cord-255350-dmbl4emn 47 27 of of IN cord-255350-dmbl4emn 47 28 grown grown JJ cord-255350-dmbl4emn 47 29 cells cell NNS cord-255350-dmbl4emn 47 30 ) ) -RRB- cord-255350-dmbl4emn 47 31 , , , cord-255350-dmbl4emn 47 32 refolded refolde VBN cord-255350-dmbl4emn 47 33 by by IN cord-255350-dmbl4emn 47 34 rapid rapid JJ cord-255350-dmbl4emn 47 35 dilution dilution NN cord-255350-dmbl4emn 47 36 ( ( -LRB- cord-255350-dmbl4emn 47 37 1:12 1:12 CD cord-255350-dmbl4emn 47 38 ratio ratio NN cord-255350-dmbl4emn 47 39 ) ) -RRB- cord-255350-dmbl4emn 47 40 at at IN cord-255350-dmbl4emn 47 41 277 277 CD cord-255350-dmbl4emn 47 42 K k NN cord-255350-dmbl4emn 47 43 into into IN cord-255350-dmbl4emn 47 44 50 50 CD cord-255350-dmbl4emn 47 45 mM mm CD cord-255350-dmbl4emn 47 46 CHES CHES NNP cord-255350-dmbl4emn 47 47 - - HYPH cord-255350-dmbl4emn 47 48 HCl HCl NNP cord-255350-dmbl4emn 47 49 , , , cord-255350-dmbl4emn 47 50 500 500 CD cord-255350-dmbl4emn 47 51 mM mm CD cord-255350-dmbl4emn 47 52 l l NN cord-255350-dmbl4emn 47 53 - - HYPH cord-255350-dmbl4emn 47 54 arginine arginine NN cord-255350-dmbl4emn 47 55 pH pH NNP cord-255350-dmbl4emn 47 56 9.2 9.2 CD cord-255350-dmbl4emn 47 57 and and CC cord-255350-dmbl4emn 47 58 left leave VBD cord-255350-dmbl4emn 47 59 for for IN cord-255350-dmbl4emn 47 60 24 24 CD cord-255350-dmbl4emn 47 61 h. h. NN cord-255350-dmbl4emn 47 62 Refolded Refolded NNP cord-255350-dmbl4emn 47 63 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 47 64 was be VBD cord-255350-dmbl4emn 47 65 dialyzed dialyze VBN cord-255350-dmbl4emn 47 66 against against IN cord-255350-dmbl4emn 47 67 10 10 CD cord-255350-dmbl4emn 47 68 mM mM NNP cord-255350-dmbl4emn 47 69 Tris Tris NNP cord-255350-dmbl4emn 47 70 - - HYPH cord-255350-dmbl4emn 47 71 HCl HCl NNP cord-255350-dmbl4emn 47 72 pH pH NNP cord-255350-dmbl4emn 47 73 8.0 8.0 CD cord-255350-dmbl4emn 47 74 and and CC cord-255350-dmbl4emn 47 75 concentrated concentrate VBN cord-255350-dmbl4emn 47 76 by by IN cord-255350-dmbl4emn 47 77 anion anion NN cord-255350-dmbl4emn 47 78 - - HYPH cord-255350-dmbl4emn 47 79 exchange exchange NN cord-255350-dmbl4emn 47 80 chromatography chromatography NN cord-255350-dmbl4emn 47 81 ( ( -LRB- cord-255350-dmbl4emn 47 82 Mono Mono NNP cord-255350-dmbl4emn 47 83 Q Q NNP cord-255350-dmbl4emn 47 84 , , , cord-255350-dmbl4emn 47 85 GE GE NNP cord-255350-dmbl4emn 47 86 Healthcare Healthcare NNP cord-255350-dmbl4emn 47 87 ) ) -RRB- cord-255350-dmbl4emn 47 88 . . . cord-255350-dmbl4emn 48 1 A a DT cord-255350-dmbl4emn 48 2 linear linear JJ cord-255350-dmbl4emn 48 3 salt salt NN cord-255350-dmbl4emn 48 4 gradient gradient NN cord-255350-dmbl4emn 48 5 from from IN cord-255350-dmbl4emn 48 6 0 0 CD cord-255350-dmbl4emn 48 7 to to IN cord-255350-dmbl4emn 48 8 1000 1000 CD cord-255350-dmbl4emn 48 9 mM mm NN cord-255350-dmbl4emn 48 10 was be VBD cord-255350-dmbl4emn 48 11 run run VBN cord-255350-dmbl4emn 48 12 at at IN cord-255350-dmbl4emn 48 13 1 1 CD cord-255350-dmbl4emn 48 14 ml ml NN cord-255350-dmbl4emn 48 15 min min NN cord-255350-dmbl4emn 48 16 À1 À1 NNP cord-255350-dmbl4emn 48 17 over over IN cord-255350-dmbl4emn 48 18 15 15 CD cord-255350-dmbl4emn 48 19 min min NN cord-255350-dmbl4emn 48 20 , , , cord-255350-dmbl4emn 48 21 with with IN cord-255350-dmbl4emn 48 22 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 48 23 eluting elute VBG cord-255350-dmbl4emn 48 24 at at IN cord-255350-dmbl4emn 48 25 between between IN cord-255350-dmbl4emn 48 26 50 50 CD cord-255350-dmbl4emn 48 27 and and CC cord-255350-dmbl4emn 48 28 100 100 CD cord-255350-dmbl4emn 48 29 mM mm CD cord-255350-dmbl4emn 48 30 NaCl nacl NN cord-255350-dmbl4emn 48 31 . . . cord-255350-dmbl4emn 49 1 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 49 2 was be VBD cord-255350-dmbl4emn 49 3 further further RB cord-255350-dmbl4emn 49 4 purified purify VBN cord-255350-dmbl4emn 49 5 by by IN cord-255350-dmbl4emn 49 6 size size NN cord-255350-dmbl4emn 49 7 - - HYPH cord-255350-dmbl4emn 49 8 exclusion exclusion NN cord-255350-dmbl4emn 49 9 chromatography chromatography NN cord-255350-dmbl4emn 49 10 ( ( -LRB- cord-255350-dmbl4emn 49 11 Superdex Superdex NNP cord-255350-dmbl4emn 49 12 200 200 CD cord-255350-dmbl4emn 49 13 , , , cord-255350-dmbl4emn 49 14 GE GE NNP cord-255350-dmbl4emn 49 15 Healthcare Healthcare NNP cord-255350-dmbl4emn 49 16 ) ) -RRB- cord-255350-dmbl4emn 49 17 in in IN cord-255350-dmbl4emn 49 18 50 50 CD cord-255350-dmbl4emn 49 19 mM mm CD cord-255350-dmbl4emn 49 20 Tris Tris NNP cord-255350-dmbl4emn 49 21 - - HYPH cord-255350-dmbl4emn 49 22 HCl HCl NNP cord-255350-dmbl4emn 49 23 , , , cord-255350-dmbl4emn 49 24 150 150 CD cord-255350-dmbl4emn 49 25 mM mm CD cord-255350-dmbl4emn 49 26 NaCl nacl NN cord-255350-dmbl4emn 49 27 , , , cord-255350-dmbl4emn 49 28 1 1 CD cord-255350-dmbl4emn 49 29 mM mm CD cord-255350-dmbl4emn 49 30 EDTA edta NN cord-255350-dmbl4emn 49 31 pH ph NN cord-255350-dmbl4emn 50 1 7.5 7.5 CD cord-255350-dmbl4emn 50 2 and and CC cord-255350-dmbl4emn 50 3 fractions fraction NNS cord-255350-dmbl4emn 50 4 were be VBD cord-255350-dmbl4emn 50 5 stored store VBN cord-255350-dmbl4emn 50 6 at at IN cord-255350-dmbl4emn 50 7 277 277 CD cord-255350-dmbl4emn 50 8 K. K. NNP cord-255350-dmbl4emn 50 9 Typically typically RB cord-255350-dmbl4emn 50 10 , , , cord-255350-dmbl4emn 50 11 10 10 CD cord-255350-dmbl4emn 50 12 mg mg NN cord-255350-dmbl4emn 50 13 of of IN cord-255350-dmbl4emn 50 14 refolded refolde VBN cord-255350-dmbl4emn 50 15 protein protein NN cord-255350-dmbl4emn 50 16 per per IN cord-255350-dmbl4emn 50 17 litre litre NN cord-255350-dmbl4emn 50 18 was be VBD cord-255350-dmbl4emn 50 19 obtained obtain VBN cord-255350-dmbl4emn 50 20 with with IN cord-255350-dmbl4emn 50 21 a a DT cord-255350-dmbl4emn 50 22 refolding refolde VBG cord-255350-dmbl4emn 50 23 efficiency efficiency NN cord-255350-dmbl4emn 50 24 of of IN cord-255350-dmbl4emn 50 25 10 10 CD cord-255350-dmbl4emn 50 26 % % NN cord-255350-dmbl4emn 50 27 . . . cord-255350-dmbl4emn 51 1 Macromolecule Macromolecule NNP cord-255350-dmbl4emn 51 2 - - HYPH cord-255350-dmbl4emn 51 3 production production NN cord-255350-dmbl4emn 51 4 information information NN cord-255350-dmbl4emn 51 5 is be VBZ cord-255350-dmbl4emn 51 6 summarized summarize VBN cord-255350-dmbl4emn 51 7 in in IN cord-255350-dmbl4emn 51 8 Table Table NNP cord-255350-dmbl4emn 51 9 1 1 CD cord-255350-dmbl4emn 51 10 . . . cord-255350-dmbl4emn 52 1 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 52 2 was be VBD cord-255350-dmbl4emn 52 3 concentrated concentrate VBN cord-255350-dmbl4emn 52 4 using use VBG cord-255350-dmbl4emn 52 5 a a DT cord-255350-dmbl4emn 52 6 Centricon Centricon NNP cord-255350-dmbl4emn 52 7 centrifugal centrifugal JJ cord-255350-dmbl4emn 52 8 filter filter NN cord-255350-dmbl4emn 52 9 unit unit NN cord-255350-dmbl4emn 52 10 ( ( -LRB- cord-255350-dmbl4emn 52 11 10 10 CD cord-255350-dmbl4emn 52 12 kDa kDa NNP cord-255350-dmbl4emn 52 13 MWCO MWCO NNP cord-255350-dmbl4emn 52 14 , , , cord-255350-dmbl4emn 52 15 Millipore Millipore NNP cord-255350-dmbl4emn 52 16 ) ) -RRB- cord-255350-dmbl4emn 52 17 and and CC cord-255350-dmbl4emn 52 18 subsequently subsequently RB cord-255350-dmbl4emn 52 19 dialyzed dialyze VBD cord-255350-dmbl4emn 52 20 against against IN cord-255350-dmbl4emn 52 21 20 20 CD cord-255350-dmbl4emn 52 22 mM mM NNP cord-255350-dmbl4emn 52 23 Tris Tris NNP cord-255350-dmbl4emn 52 24 - - HYPH cord-255350-dmbl4emn 52 25 HCl HCl NNP cord-255350-dmbl4emn 52 26 , , , cord-255350-dmbl4emn 52 27 100 100 CD cord-255350-dmbl4emn 52 28 mM mm CD cord-255350-dmbl4emn 52 29 NaCl nacl NN cord-255350-dmbl4emn 52 30 pH pH NNP cord-255350-dmbl4emn 52 31 7.5 7.5 CD cord-255350-dmbl4emn 52 32 . . . cord-255350-dmbl4emn 53 1 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 54 1 at at IN cord-255350-dmbl4emn 54 2 5.9 5.9 CD cord-255350-dmbl4emn 54 3 mg mg NN cord-255350-dmbl4emn 54 4 ml ml NN cord-255350-dmbl4emn 54 5 À1 À1 NNP cord-255350-dmbl4emn 54 6 was be VBD cord-255350-dmbl4emn 54 7 screened screen VBN cord-255350-dmbl4emn 54 8 against against IN cord-255350-dmbl4emn 54 9 The the DT cord-255350-dmbl4emn 54 10 JCSG+ JCSG+ NNP cord-255350-dmbl4emn 54 11 Suite Suite NNP cord-255350-dmbl4emn 54 12 screen screen NN cord-255350-dmbl4emn 54 13 ( ( -LRB- cord-255350-dmbl4emn 54 14 Qiagen Qiagen NNP cord-255350-dmbl4emn 54 15 ) ) -RRB- cord-255350-dmbl4emn 54 16 using use VBG cord-255350-dmbl4emn 54 17 a a DT cord-255350-dmbl4emn 54 18 Crystal Crystal NNP cord-255350-dmbl4emn 54 19 Gryphon Gryphon NNP cord-255350-dmbl4emn 54 20 Protein Protein NNP cord-255350-dmbl4emn 54 21 Crystallography Crystallography NNP cord-255350-dmbl4emn 54 22 System System NNP cord-255350-dmbl4emn 54 23 ( ( -LRB- cord-255350-dmbl4emn 54 24 Art Art NNP cord-255350-dmbl4emn 54 25 Robbins Robbins NNP cord-255350-dmbl4emn 54 26 Instruments Instruments NNPS cord-255350-dmbl4emn 54 27 ) ) -RRB- cord-255350-dmbl4emn 54 28 with with IN cord-255350-dmbl4emn 54 29 sitting sit VBG cord-255350-dmbl4emn 54 30 drops drop NNS cord-255350-dmbl4emn 54 31 consisting consist VBG cord-255350-dmbl4emn 54 32 of of IN cord-255350-dmbl4emn 54 33 150 150 CD cord-255350-dmbl4emn 54 34 nl nl NNP cord-255350-dmbl4emn 54 35 protein protein NN cord-255350-dmbl4emn 54 36 solution solution NN cord-255350-dmbl4emn 54 37 and and CC cord-255350-dmbl4emn 54 38 150 150 CD cord-255350-dmbl4emn 54 39 nl nl NNP cord-255350-dmbl4emn 54 40 reservoir reservoir NN cord-255350-dmbl4emn 54 41 solution solution NN cord-255350-dmbl4emn 54 42 equilibrated equilibrate VBN cord-255350-dmbl4emn 54 43 against against IN cord-255350-dmbl4emn 54 44 50 50 CD cord-255350-dmbl4emn 54 45 ml ml NN cord-255350-dmbl4emn 54 46 reservoir reservoir NN cord-255350-dmbl4emn 54 47 solution solution NN cord-255350-dmbl4emn 54 48 . . . cord-255350-dmbl4emn 55 1 A a DT cord-255350-dmbl4emn 55 2 shower shower NN cord-255350-dmbl4emn 55 3 of of IN cord-255350-dmbl4emn 55 4 small small JJ cord-255350-dmbl4emn 55 5 crystals crystal NNS cord-255350-dmbl4emn 55 6 grew grow VBD cord-255350-dmbl4emn 55 7 over over IN cord-255350-dmbl4emn 55 8 5 5 CD cord-255350-dmbl4emn 55 9 d d NN cord-255350-dmbl4emn 55 10 in in IN cord-255350-dmbl4emn 55 11 condition condition NN cord-255350-dmbl4emn 55 12 D6 d6 NN cord-255350-dmbl4emn 55 13 _SP cord-255350-dmbl4emn 55 14 Crystals crystal NNS cord-255350-dmbl4emn 55 15 of of IN cord-255350-dmbl4emn 55 16 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 55 17 were be VBD cord-255350-dmbl4emn 55 18 washed wash VBN cord-255350-dmbl4emn 55 19 and and CC cord-255350-dmbl4emn 55 20 cryoprotected cryoprotecte VBN cord-255350-dmbl4emn 55 21 in in IN cord-255350-dmbl4emn 55 22 mother mother NN cord-255350-dmbl4emn 55 23 liquor liquor NN cord-255350-dmbl4emn 55 24 containing contain VBG cord-255350-dmbl4emn 55 25 20%(v 20%(v CD cord-255350-dmbl4emn 55 26 / / SYM cord-255350-dmbl4emn 55 27 v v NN cord-255350-dmbl4emn 55 28 ) ) -RRB- cord-255350-dmbl4emn 55 29 glycerol glycerol NN cord-255350-dmbl4emn 55 30 . . . cord-255350-dmbl4emn 56 1 Data datum NNS cord-255350-dmbl4emn 56 2 were be VBD cord-255350-dmbl4emn 56 3 collected collect VBN cord-255350-dmbl4emn 56 4 on on IN cord-255350-dmbl4emn 56 5 beamline beamline NN cord-255350-dmbl4emn 56 6 23-ID 23-id CD cord-255350-dmbl4emn 56 7 - - HYPH cord-255350-dmbl4emn 56 8 B b NN cord-255350-dmbl4emn 56 9 at at IN cord-255350-dmbl4emn 56 10 the the DT cord-255350-dmbl4emn 56 11 Advanced Advanced NNP cord-255350-dmbl4emn 56 12 Photon Photon NNP cord-255350-dmbl4emn 56 13 Source Source NNP cord-255350-dmbl4emn 56 14 ( ( -LRB- cord-255350-dmbl4emn 56 15 APS APS NNP cord-255350-dmbl4emn 56 16 ) ) -RRB- cord-255350-dmbl4emn 56 17 , , , cord-255350-dmbl4emn 56 18 Argonne Argonne NNP cord-255350-dmbl4emn 56 19 National National NNP cord-255350-dmbl4emn 56 20 Laboratory Laboratory NNP cord-255350-dmbl4emn 56 21 , , , cord-255350-dmbl4emn 56 22 USA USA NNP cord-255350-dmbl4emn 56 23 . . . cord-255350-dmbl4emn 57 1 Data datum NNS cord-255350-dmbl4emn 57 2 were be VBD cord-255350-dmbl4emn 57 3 processed process VBN cord-255350-dmbl4emn 57 4 using use VBG cord-255350-dmbl4emn 57 5 HKL-2000 HKL-2000 NNP cord-255350-dmbl4emn 57 6 ( ( -LRB- cord-255350-dmbl4emn 57 7 Otwinowski Otwinowski NNP cord-255350-dmbl4emn 57 8 & & CC cord-255350-dmbl4emn 57 9 Minor Minor NNP cord-255350-dmbl4emn 57 10 , , , cord-255350-dmbl4emn 57 11 1997 1997 CD cord-255350-dmbl4emn 57 12 ) ) -RRB- cord-255350-dmbl4emn 57 13 . . . cord-255350-dmbl4emn 58 1 Data data NN cord-255350-dmbl4emn 58 2 - - HYPH cord-255350-dmbl4emn 58 3 collection collection NN cord-255350-dmbl4emn 58 4 and and CC cord-255350-dmbl4emn 58 5 processing processing NN cord-255350-dmbl4emn 58 6 statistics statistic NNS cord-255350-dmbl4emn 58 7 are be VBP cord-255350-dmbl4emn 58 8 shown show VBN cord-255350-dmbl4emn 58 9 in in IN cord-255350-dmbl4emn 58 10 Table Table NNP cord-255350-dmbl4emn 58 11 3 3 CD cord-255350-dmbl4emn 58 12 . . . cord-255350-dmbl4emn 59 1 F F NNP cord-255350-dmbl4emn 59 2 obs obs NNP cord-255350-dmbl4emn 59 3 were be VBD cord-255350-dmbl4emn 59 4 obtained obtain VBN cord-255350-dmbl4emn 59 5 using use VBG cord-255350-dmbl4emn 59 6 SCALEPACK2MTZ SCALEPACK2MTZ NNS cord-255350-dmbl4emn 59 7 . . . cord-255350-dmbl4emn 60 1 Molecular molecular JJ cord-255350-dmbl4emn 60 2 replacement replacement NN cord-255350-dmbl4emn 60 3 was be VBD cord-255350-dmbl4emn 60 4 performed perform VBN cord-255350-dmbl4emn 60 5 using use VBG cord-255350-dmbl4emn 60 6 MOLREP MOLREP NNP cord-255350-dmbl4emn 60 7 ( ( -LRB- cord-255350-dmbl4emn 60 8 Vagin Vagin NNP cord-255350-dmbl4emn 60 9 & & CC cord-255350-dmbl4emn 60 10 Teplyakov Teplyakov NNP cord-255350-dmbl4emn 60 11 , , , cord-255350-dmbl4emn 60 12 2010 2010 CD cord-255350-dmbl4emn 60 13 ) ) -RRB- cord-255350-dmbl4emn 60 14 and and CC cord-255350-dmbl4emn 60 15 a a DT cord-255350-dmbl4emn 60 16 CHAINSAW CHAINSAW NNP cord-255350-dmbl4emn 60 17 ( ( -LRB- cord-255350-dmbl4emn 60 18 Stein Stein NNP cord-255350-dmbl4emn 60 19 , , , cord-255350-dmbl4emn 60 20 2008 2008 CD cord-255350-dmbl4emn 60 21 ) ) -RRB- cord-255350-dmbl4emn 60 22 model model NN cord-255350-dmbl4emn 60 23 of of IN cord-255350-dmbl4emn 60 24 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 61 1 ( ( -LRB- cord-255350-dmbl4emn 61 2 PDB PDB NNP cord-255350-dmbl4emn 61 3 entry entry NN cord-255350-dmbl4emn 61 4 2qsq 2qsq CD cord-255350-dmbl4emn 61 5 ; ; : cord-255350-dmbl4emn 62 1 Korotkova Korotkova NNP cord-255350-dmbl4emn 62 2 et et FW cord-255350-dmbl4emn 62 3 al al NNP cord-255350-dmbl4emn 62 4 . . NNP cord-255350-dmbl4emn 62 5 , , , cord-255350-dmbl4emn 62 6 2008 2008 CD cord-255350-dmbl4emn 62 7 ) ) -RRB- cord-255350-dmbl4emn 62 8 , , , cord-255350-dmbl4emn 62 9 a a DT cord-255350-dmbl4emn 62 10 protein protein NN cord-255350-dmbl4emn 62 11 with with IN cord-255350-dmbl4emn 62 12 65 65 CD cord-255350-dmbl4emn 62 13 % % NN cord-255350-dmbl4emn 62 14 sequence sequence NN cord-255350-dmbl4emn 62 15 identity identity NN cord-255350-dmbl4emn 62 16 to to IN cord-255350-dmbl4emn 62 17 CEACAM7 ceacam7 XX cord-255350-dmbl4emn 62 18 . . . cord-255350-dmbl4emn 63 1 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 63 2 was be VBD cord-255350-dmbl4emn 63 3 refined refine VBN cord-255350-dmbl4emn 63 4 with with IN cord-255350-dmbl4emn 63 5 REFMAC REFMAC NNP cord-255350-dmbl4emn 63 6 ( ( -LRB- cord-255350-dmbl4emn 63 7 Murshudov Murshudov NNP cord-255350-dmbl4emn 63 8 et et NNP cord-255350-dmbl4emn 63 9 al al NNP cord-255350-dmbl4emn 63 10 . . NNP cord-255350-dmbl4emn 63 11 , , , cord-255350-dmbl4emn 63 12 2011 2011 CD cord-255350-dmbl4emn 63 13 ) ) -RRB- cord-255350-dmbl4emn 63 14 and and CC cord-255350-dmbl4emn 63 15 rebuilt rebuild VBN cord-255350-dmbl4emn 63 16 in in IN cord-255350-dmbl4emn 63 17 Coot Coot NNP cord-255350-dmbl4emn 63 18 ( ( -LRB- cord-255350-dmbl4emn 63 19 Emsley Emsley NNP cord-255350-dmbl4emn 63 20 et et FW cord-255350-dmbl4emn 63 21 al al NNP cord-255350-dmbl4emn 63 22 . . NNP cord-255350-dmbl4emn 63 23 , , , cord-255350-dmbl4emn 63 24 2010 2010 CD cord-255350-dmbl4emn 63 25 ) ) -RRB- cord-255350-dmbl4emn 63 26 . . . cord-255350-dmbl4emn 64 1 MolProbity MolProbity NNP cord-255350-dmbl4emn 65 1 ( ( -LRB- cord-255350-dmbl4emn 65 2 Chen Chen NNP cord-255350-dmbl4emn 65 3 et et NNP cord-255350-dmbl4emn 65 4 al al NNP cord-255350-dmbl4emn 65 5 . . NNP cord-255350-dmbl4emn 65 6 , , , cord-255350-dmbl4emn 65 7 2010 2010 CD cord-255350-dmbl4emn 65 8 ) ) -RRB- cord-255350-dmbl4emn 65 9 was be VBD cord-255350-dmbl4emn 65 10 used use VBN cord-255350-dmbl4emn 65 11 for for IN cord-255350-dmbl4emn 65 12 Ramachandran Ramachandran NNP cord-255350-dmbl4emn 65 13 analysis analysis NN cord-255350-dmbl4emn 65 14 . . . cord-255350-dmbl4emn 66 1 Refinement refinement NN cord-255350-dmbl4emn 66 2 statistics statistic NNS cord-255350-dmbl4emn 66 3 are be VBP cord-255350-dmbl4emn 66 4 shown show VBN cord-255350-dmbl4emn 66 5 in in IN cord-255350-dmbl4emn 66 6 Table Table NNP cord-255350-dmbl4emn 66 7 4 4 CD cord-255350-dmbl4emn 66 8 . . . cord-255350-dmbl4emn 67 1 Sedimentation sedimentation NN cord-255350-dmbl4emn 67 2 - - HYPH cord-255350-dmbl4emn 67 3 equilibrium equilibrium NN cord-255350-dmbl4emn 67 4 measurements measurement NNS cord-255350-dmbl4emn 67 5 of of IN cord-255350-dmbl4emn 67 6 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 67 7 were be VBD cord-255350-dmbl4emn 67 8 performed perform VBN cord-255350-dmbl4emn 67 9 using use VBG cord-255350-dmbl4emn 67 10 a a DT cord-255350-dmbl4emn 67 11 Beckman Beckman NNP cord-255350-dmbl4emn 67 12 - - HYPH cord-255350-dmbl4emn 67 13 Coulter Coulter NNP cord-255350-dmbl4emn 67 14 XL XL NNP cord-255350-dmbl4emn 67 15 - - : cord-255350-dmbl4emn 67 16 I -PRON- PRP cord-255350-dmbl4emn 67 17 analytical analytical JJ cord-255350-dmbl4emn 67 18 ultracentrifuge ultracentrifuge NN cord-255350-dmbl4emn 67 19 equipped equip VBN cord-255350-dmbl4emn 67 20 with with IN cord-255350-dmbl4emn 67 21 a a DT cord-255350-dmbl4emn 67 22 four four CD cord-255350-dmbl4emn 67 23 - - HYPH cord-255350-dmbl4emn 67 24 hole hole NN cord-255350-dmbl4emn 68 1 An-60 An-60 NNS cord-255350-dmbl4emn 69 1 Ti Ti NNP cord-255350-dmbl4emn 69 2 rotor rotor VB cord-255350-dmbl4emn 69 3 at at IN cord-255350-dmbl4emn 69 4 20 20 CD cord-255350-dmbl4emn 69 5 C. C. NNP cord-255350-dmbl4emn 69 6 Prior Prior NNP cord-255350-dmbl4emn 69 7 to to IN cord-255350-dmbl4emn 69 8 centrifugation centrifugation NN cord-255350-dmbl4emn 69 9 , , , cord-255350-dmbl4emn 69 10 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 69 11 was be VBD cord-255350-dmbl4emn 69 12 dialyzed dialyze VBN cord-255350-dmbl4emn 69 13 extensively extensively RB cord-255350-dmbl4emn 69 14 against against IN cord-255350-dmbl4emn 69 15 50 50 CD cord-255350-dmbl4emn 69 16 mM mm CD cord-255350-dmbl4emn 69 17 Tris Tris NNP cord-255350-dmbl4emn 69 18 - - HYPH cord-255350-dmbl4emn 69 19 HCl HCl NNP cord-255350-dmbl4emn 69 20 pH pH NNP cord-255350-dmbl4emn 69 21 7.5 7.5 CD cord-255350-dmbl4emn 69 22 , , , cord-255350-dmbl4emn 69 23 50 50 CD cord-255350-dmbl4emn 69 24 mM mm CD cord-255350-dmbl4emn 69 25 NaCl nacl NN cord-255350-dmbl4emn 69 26 . . . cord-255350-dmbl4emn 70 1 SEDNTERP SEDNTERP NNP cord-255350-dmbl4emn 70 2 ( ( -LRB- cord-255350-dmbl4emn 70 3 http://sednterp.unh.edu http://sednterp.unh.edu NN cord-255350-dmbl4emn 70 4 ) ) -RRB- cord-255350-dmbl4emn 70 5 was be VBD cord-255350-dmbl4emn 70 6 used use VBN cord-255350-dmbl4emn 70 7 to to TO cord-255350-dmbl4emn 70 8 calculate calculate VB cord-255350-dmbl4emn 70 9 values value NNS cord-255350-dmbl4emn 70 10 for for IN cord-255350-dmbl4emn 70 11 the the DT cord-255350-dmbl4emn 70 12 protein protein NN cord-255350-dmbl4emn 70 13 partial partial JJ cord-255350-dmbl4emn 70 14 specific specific JJ cord-255350-dmbl4emn 70 15 volume volume NN cord-255350-dmbl4emn 70 16 and and CC cord-255350-dmbl4emn 70 17 solvent solvent NN cord-255350-dmbl4emn 70 18 density density NN cord-255350-dmbl4emn 70 19 from from IN cord-255350-dmbl4emn 70 20 the the DT cord-255350-dmbl4emn 70 21 protein protein NN cord-255350-dmbl4emn 70 22 amino amino NN cord-255350-dmbl4emn 70 23 - - HYPH cord-255350-dmbl4emn 70 24 acid acid NN cord-255350-dmbl4emn 70 25 sequence sequence NN cord-255350-dmbl4emn 70 26 and and CC cord-255350-dmbl4emn 70 27 buffer buffer NN cord-255350-dmbl4emn 70 28 composition composition NN cord-255350-dmbl4emn 70 29 , , , cord-255350-dmbl4emn 70 30 respectively respectively RB cord-255350-dmbl4emn 70 31 . . . cord-255350-dmbl4emn 71 1 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 72 1 at at IN cord-255350-dmbl4emn 72 2 three three CD cord-255350-dmbl4emn 72 3 different different JJ cord-255350-dmbl4emn 72 4 concentrations concentration NNS cord-255350-dmbl4emn 72 5 ( ( -LRB- cord-255350-dmbl4emn 72 6 24.1 24.1 CD cord-255350-dmbl4emn 72 7 , , , cord-255350-dmbl4emn 72 8 14.5 14.5 CD cord-255350-dmbl4emn 72 9 and and CC cord-255350-dmbl4emn 72 10 9.6 9.6 CD cord-255350-dmbl4emn 72 11 mM mm CD cord-255350-dmbl4emn 72 12 ) ) -RRB- cord-255350-dmbl4emn 72 13 was be VBD cord-255350-dmbl4emn 72 14 loaded load VBN cord-255350-dmbl4emn 72 15 into into IN cord-255350-dmbl4emn 72 16 cells cell NNS cord-255350-dmbl4emn 72 17 equipped equip VBN cord-255350-dmbl4emn 72 18 with with IN cord-255350-dmbl4emn 72 19 six six CD cord-255350-dmbl4emn 72 20 - - HYPH cord-255350-dmbl4emn 72 21 hole hole NN cord-255350-dmbl4emn 72 22 charcoal charcoal NN cord-255350-dmbl4emn 72 23 - - HYPH cord-255350-dmbl4emn 72 24 filled fill VBN cord-255350-dmbl4emn 72 25 Epon Epon NNP cord-255350-dmbl4emn 72 26 centerpieces centerpiece NNS cord-255350-dmbl4emn 72 27 ( ( -LRB- cord-255350-dmbl4emn 72 28 1.2 1.2 CD cord-255350-dmbl4emn 72 29 cm cm NN cord-255350-dmbl4emn 72 30 path path NN cord-255350-dmbl4emn 72 31 length length NN cord-255350-dmbl4emn 72 32 ) ) -RRB- cord-255350-dmbl4emn 72 33 with with IN cord-255350-dmbl4emn 72 34 sapphire sapphire NN cord-255350-dmbl4emn 72 35 windows window NNS cord-255350-dmbl4emn 72 36 . . . cord-255350-dmbl4emn 73 1 Centrifugation Centrifugation NNP cord-255350-dmbl4emn 73 2 was be VBD cord-255350-dmbl4emn 73 3 carried carry VBN cord-255350-dmbl4emn 73 4 out out RP cord-255350-dmbl4emn 73 5 at at IN cord-255350-dmbl4emn 73 6 29 29 CD cord-255350-dmbl4emn 73 7 000 000 CD cord-255350-dmbl4emn 73 8 , , , cord-255350-dmbl4emn 73 9 32 32 CD cord-255350-dmbl4emn 73 10 000 000 CD cord-255350-dmbl4emn 73 11 and and CC cord-255350-dmbl4emn 73 12 35 35 CD cord-255350-dmbl4emn 73 13 000 000 CD cord-255350-dmbl4emn 73 14 rev rev NN cord-255350-dmbl4emn 73 15 min min NNP cord-255350-dmbl4emn 73 16 À1 À1 NNP cord-255350-dmbl4emn 73 17 and and CC cord-255350-dmbl4emn 73 18 scans scan NNS cord-255350-dmbl4emn 73 19 were be VBD cord-255350-dmbl4emn 73 20 acquired acquire VBN cord-255350-dmbl4emn 73 21 at at IN cord-255350-dmbl4emn 73 22 280 280 CD cord-255350-dmbl4emn 73 23 nm nm NN cord-255350-dmbl4emn 73 24 with with IN cord-255350-dmbl4emn 73 25 a a DT cord-255350-dmbl4emn 73 26 step step NN cord-255350-dmbl4emn 73 27 size size NN cord-255350-dmbl4emn 73 28 of of IN cord-255350-dmbl4emn 73 29 0.001 0.001 CD cord-255350-dmbl4emn 73 30 and and CC cord-255350-dmbl4emn 73 31 five five CD cord-255350-dmbl4emn 73 32 averages average NNS cord-255350-dmbl4emn 73 33 per per IN cord-255350-dmbl4emn 73 34 step step NN cord-255350-dmbl4emn 73 35 . . . cord-255350-dmbl4emn 74 1 The the DT cord-255350-dmbl4emn 74 2 data datum NNS cord-255350-dmbl4emn 74 3 were be VBD cord-255350-dmbl4emn 74 4 globally globally RB cord-255350-dmbl4emn 74 5 analyzed analyze VBN cord-255350-dmbl4emn 74 6 using use VBG cord-255350-dmbl4emn 74 7 the the DT cord-255350-dmbl4emn 74 8 WinNonLin WinNonLin NNP cord-255350-dmbl4emn 74 9 program program NN cord-255350-dmbl4emn 74 10 ( ( -LRB- cord-255350-dmbl4emn 74 11 Johnson Johnson NNP cord-255350-dmbl4emn 74 12 et et NNP cord-255350-dmbl4emn 74 13 al al NNP cord-255350-dmbl4emn 74 14 . . NNP cord-255350-dmbl4emn 74 15 , , , cord-255350-dmbl4emn 74 16 1981 1981 CD cord-255350-dmbl4emn 74 17 ) ) -RRB- cord-255350-dmbl4emn 74 18 . . . cord-255350-dmbl4emn 75 1 A a DT cord-255350-dmbl4emn 75 2 single single JJ cord-255350-dmbl4emn 75 3 X x NN cord-255350-dmbl4emn 75 4 - - NN cord-255350-dmbl4emn 75 5 ray ray NN cord-255350-dmbl4emn 75 6 diffraction diffraction NN cord-255350-dmbl4emn 75 7 data datum NNS cord-255350-dmbl4emn 75 8 set set NN cord-255350-dmbl4emn 75 9 was be VBD cord-255350-dmbl4emn 75 10 collected collect VBN cord-255350-dmbl4emn 75 11 to to IN cord-255350-dmbl4emn 75 12 a a DT cord-255350-dmbl4emn 75 13 resolution resolution NN cord-255350-dmbl4emn 75 14 of of IN cord-255350-dmbl4emn 75 15 1.47 1.47 CD cord-255350-dmbl4emn 75 16 Å å NN cord-255350-dmbl4emn 75 17 . . . cord-255350-dmbl4emn 76 1 Initial initial JJ cord-255350-dmbl4emn 76 2 indexing indexing NN cord-255350-dmbl4emn 76 3 of of IN cord-255350-dmbl4emn 76 4 the the DT cord-255350-dmbl4emn 76 5 data datum NNS cord-255350-dmbl4emn 76 6 suggested suggest VBD cord-255350-dmbl4emn 76 7 that that IN cord-255350-dmbl4emn 76 8 the the DT cord-255350-dmbl4emn 76 9 crystal crystal NN cord-255350-dmbl4emn 76 10 contained contain VBD cord-255350-dmbl4emn 76 11 a a DT cord-255350-dmbl4emn 76 12 primitive primitive JJ cord-255350-dmbl4emn 76 13 orthorhombic orthorhombic JJ cord-255350-dmbl4emn 76 14 lattice lattice NN cord-255350-dmbl4emn 76 15 with with IN cord-255350-dmbl4emn 76 16 two two CD cord-255350-dmbl4emn 76 17 molecules molecule NNS cord-255350-dmbl4emn 76 18 in in IN cord-255350-dmbl4emn 76 19 the the DT cord-255350-dmbl4emn 76 20 asymmetric asymmetric JJ cord-255350-dmbl4emn 76 21 unit unit NN cord-255350-dmbl4emn 76 22 . . . cord-255350-dmbl4emn 77 1 However however RB cord-255350-dmbl4emn 77 2 , , , cord-255350-dmbl4emn 77 3 attempts attempt VBZ cord-255350-dmbl4emn 77 4 to to TO cord-255350-dmbl4emn 77 5 find find VB cord-255350-dmbl4emn 77 6 a a DT cord-255350-dmbl4emn 77 7 molecular molecular JJ cord-255350-dmbl4emn 77 8 - - HYPH cord-255350-dmbl4emn 77 9 replacement replacement NN cord-255350-dmbl4emn 77 10 solution solution NN cord-255350-dmbl4emn 77 11 using use VBG cord-255350-dmbl4emn 77 12 the the DT cord-255350-dmbl4emn 77 13 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 77 14 monomer monomer NN cord-255350-dmbl4emn 77 15 as as IN cord-255350-dmbl4emn 77 16 a a DT cord-255350-dmbl4emn 77 17 search search NN cord-255350-dmbl4emn 77 18 model model NN cord-255350-dmbl4emn 77 19 yielded yield VBD cord-255350-dmbl4emn 77 20 no no DT cord-255350-dmbl4emn 77 21 solutions solution NNS cord-255350-dmbl4emn 77 22 that that WDT cord-255350-dmbl4emn 77 23 would would MD cord-255350-dmbl4emn 77 24 refine refine VB cord-255350-dmbl4emn 77 25 in in IN cord-255350-dmbl4emn 77 26 any any DT cord-255350-dmbl4emn 77 27 of of IN cord-255350-dmbl4emn 77 28 the the DT cord-255350-dmbl4emn 77 29 orthorhombic orthorhombic JJ cord-255350-dmbl4emn 77 30 space space NN cord-255350-dmbl4emn 77 31 groups group NNS cord-255350-dmbl4emn 77 32 . . . cord-255350-dmbl4emn 78 1 The the DT cord-255350-dmbl4emn 78 2 data datum NNS cord-255350-dmbl4emn 78 3 were be VBD cord-255350-dmbl4emn 78 4 reprocessed reprocess VBN cord-255350-dmbl4emn 78 5 in in IN cord-255350-dmbl4emn 78 6 a a DT cord-255350-dmbl4emn 78 7 primitive primitive JJ cord-255350-dmbl4emn 78 8 monoclinic monoclinic JJ cord-255350-dmbl4emn 78 9 lattice lattice NN cord-255350-dmbl4emn 78 10 with with IN cord-255350-dmbl4emn 78 11 a a DT cord-255350-dmbl4emn 78 12 angle angle NN cord-255350-dmbl4emn 78 13 of of IN cord-255350-dmbl4emn 78 14 89.99 89.99 CD cord-255350-dmbl4emn 78 15 , , , cord-255350-dmbl4emn 78 16 which which WDT cord-255350-dmbl4emn 78 17 led lead VBD cord-255350-dmbl4emn 78 18 to to IN cord-255350-dmbl4emn 78 19 the the DT cord-255350-dmbl4emn 78 20 correct correct JJ cord-255350-dmbl4emn 78 21 solution solution NN cord-255350-dmbl4emn 78 22 in in IN cord-255350-dmbl4emn 78 23 space space NN cord-255350-dmbl4emn 78 24 group group NN cord-255350-dmbl4emn 78 25 P2 P2 NNP cord-255350-dmbl4emn 78 26 1 1 CD cord-255350-dmbl4emn 78 27 with with IN cord-255350-dmbl4emn 78 28 four four CD cord-255350-dmbl4emn 78 29 copies copy NNS cord-255350-dmbl4emn 78 30 of of IN cord-255350-dmbl4emn 78 31 the the DT cord-255350-dmbl4emn 78 32 search search NN cord-255350-dmbl4emn 78 33 model model NN cord-255350-dmbl4emn 78 34 found find VBN cord-255350-dmbl4emn 78 35 in in IN cord-255350-dmbl4emn 78 36 the the DT cord-255350-dmbl4emn 78 37 asymmetric asymmetric JJ cord-255350-dmbl4emn 78 38 unit unit NN cord-255350-dmbl4emn 78 39 . . . cord-255350-dmbl4emn 79 1 h|L|i h|L|i NNP cord-255350-dmbl4emn 79 2 tests test NNS cord-255350-dmbl4emn 79 3 of of IN cord-255350-dmbl4emn 79 4 the the DT cord-255350-dmbl4emn 79 5 data datum NNS cord-255350-dmbl4emn 79 6 ( ( -LRB- cord-255350-dmbl4emn 79 7 0.503 0.503 CD cord-255350-dmbl4emn 79 8 ) ) -RRB- cord-255350-dmbl4emn 79 9 show show VBP cord-255350-dmbl4emn 79 10 that that IN cord-255350-dmbl4emn 79 11 the the DT cord-255350-dmbl4emn 79 12 data datum NNS cord-255350-dmbl4emn 79 13 are be VBP cord-255350-dmbl4emn 79 14 untwinned untwinne VBN cord-255350-dmbl4emn 79 15 and and CC cord-255350-dmbl4emn 79 16 no no DT cord-255350-dmbl4emn 79 17 pseudo pseudo JJ cord-255350-dmbl4emn 79 18 - - JJ cord-255350-dmbl4emn 79 19 merohedral merohedral JJ cord-255350-dmbl4emn 79 20 twinning twinning NN cord-255350-dmbl4emn 79 21 was be VBD cord-255350-dmbl4emn 79 22 detected detect VBN cord-255350-dmbl4emn 79 23 . . . cord-255350-dmbl4emn 80 1 All all DT cord-255350-dmbl4emn 80 2 residues residue NNS cord-255350-dmbl4emn 80 3 in in IN cord-255350-dmbl4emn 80 4 the the DT cord-255350-dmbl4emn 80 5 final final JJ cord-255350-dmbl4emn 80 6 model model NN cord-255350-dmbl4emn 80 7 were be VBD cord-255350-dmbl4emn 80 8 modeled model VBN cord-255350-dmbl4emn 80 9 except except IN cord-255350-dmbl4emn 80 10 for for IN cord-255350-dmbl4emn 80 11 the the DT cord-255350-dmbl4emn 80 12 initial initial JJ cord-255350-dmbl4emn 80 13 alanines alanine NNS cord-255350-dmbl4emn 80 14 of of IN cord-255350-dmbl4emn 80 15 two two CD cord-255350-dmbl4emn 80 16 of of IN cord-255350-dmbl4emn 80 17 the the DT cord-255350-dmbl4emn 80 18 four four CD cord-255350-dmbl4emn 80 19 molecules molecule NNS cord-255350-dmbl4emn 80 20 in in IN cord-255350-dmbl4emn 80 21 the the DT cord-255350-dmbl4emn 80 22 asymmetric asymmetric JJ cord-255350-dmbl4emn 80 23 unit unit NN cord-255350-dmbl4emn 80 24 . . . cord-255350-dmbl4emn 81 1 The the DT cord-255350-dmbl4emn 81 2 final final JJ cord-255350-dmbl4emn 81 3 model model NN cord-255350-dmbl4emn 81 4 contained contain VBD cord-255350-dmbl4emn 81 5 three three CD cord-255350-dmbl4emn 81 6 chloride chloride NN cord-255350-dmbl4emn 81 7 ions ion NNS cord-255350-dmbl4emn 81 8 and and CC cord-255350-dmbl4emn 81 9 269 269 CD cord-255350-dmbl4emn 81 10 waters water NNS cord-255350-dmbl4emn 81 11 . . . cord-255350-dmbl4emn 82 1 The the DT cord-255350-dmbl4emn 82 2 final final JJ cord-255350-dmbl4emn 82 3 model model NN cord-255350-dmbl4emn 82 4 was be VBD cord-255350-dmbl4emn 82 5 refined refine VBN cord-255350-dmbl4emn 82 6 to to IN cord-255350-dmbl4emn 82 7 an an DT cord-255350-dmbl4emn 82 8 R r NN cord-255350-dmbl4emn 82 9 cryst cryst NN cord-255350-dmbl4emn 82 10 and and CC cord-255350-dmbl4emn 82 11 R r NN cord-255350-dmbl4emn 82 12 free free JJ cord-255350-dmbl4emn 82 13 of of IN cord-255350-dmbl4emn 82 14 0.143 0.143 CD cord-255350-dmbl4emn 82 15 and and CC cord-255350-dmbl4emn 82 16 0.193 0.193 CD cord-255350-dmbl4emn 82 17 , , , cord-255350-dmbl4emn 82 18 respectively respectively RB cord-255350-dmbl4emn 82 19 . . . cord-255350-dmbl4emn 83 1 The the DT cord-255350-dmbl4emn 83 2 structure structure NN cord-255350-dmbl4emn 83 3 factors factor NNS cord-255350-dmbl4emn 83 4 and and CC cord-255350-dmbl4emn 83 5 model model NN cord-255350-dmbl4emn 83 6 have have VBP cord-255350-dmbl4emn 83 7 been be VBN cord-255350-dmbl4emn 83 8 deposited deposit VBN cord-255350-dmbl4emn 83 9 in in IN cord-255350-dmbl4emn 83 10 the the DT cord-255350-dmbl4emn 83 11 Protein Protein NNP cord-255350-dmbl4emn 83 12 Data Data NNP cord-255350-dmbl4emn 83 13 Bank Bank NNP cord-255350-dmbl4emn 83 14 ( ( -LRB- cord-255350-dmbl4emn 83 15 PDB PDB NNP cord-255350-dmbl4emn 83 16 entry entry NN cord-255350-dmbl4emn 83 17 4y89 4y89 CD cord-255350-dmbl4emn 83 18 ) ) -RRB- cord-255350-dmbl4emn 83 19 . . . cord-255350-dmbl4emn 84 1 The the DT cord-255350-dmbl4emn 84 2 closest close JJS cord-255350-dmbl4emn 84 3 homologs homolog NNS cord-255350-dmbl4emn 84 4 of of IN cord-255350-dmbl4emn 84 5 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 84 6 in in IN cord-255350-dmbl4emn 84 7 the the DT cord-255350-dmbl4emn 84 8 PDB PDB NNP cord-255350-dmbl4emn 84 9 are be VBP cord-255350-dmbl4emn 84 10 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 84 11 and and CC cord-255350-dmbl4emn 84 12 CEACAM5 CEACAM5 NNP cord-255350-dmbl4emn 84 13 , , , cord-255350-dmbl4emn 84 14 which which WDT cord-255350-dmbl4emn 84 15 both both DT cord-255350-dmbl4emn 84 16 share share VBP cord-255350-dmbl4emn 84 17 65 65 CD cord-255350-dmbl4emn 84 18 % % NN cord-255350-dmbl4emn 84 19 sequence sequence NN cord-255350-dmbl4emn 84 20 identity identity NN cord-255350-dmbl4emn 84 21 with with IN cord-255350-dmbl4emn 84 22 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 84 23 , , , cord-255350-dmbl4emn 84 24 with with IN cord-255350-dmbl4emn 84 25 38 38 CD cord-255350-dmbl4emn 84 26 residues residue NNS cord-255350-dmbl4emn 84 27 differing differ VBG cord-255350-dmbl4emn 84 28 between between IN cord-255350-dmbl4emn 84 29 the the DT cord-255350-dmbl4emn 84 30 proteins protein NNS cord-255350-dmbl4emn 84 31 ( ( -LRB- cord-255350-dmbl4emn 84 32 Fig Fig NNP cord-255350-dmbl4emn 84 33 . . . cord-255350-dmbl4emn 84 34 1a 1a CD cord-255350-dmbl4emn 84 35 ) ) -RRB- cord-255350-dmbl4emn 84 36 . . . cord-255350-dmbl4emn 85 1 The the DT cord-255350-dmbl4emn 85 2 overall overall JJ cord-255350-dmbl4emn 85 3 fold fold NN cord-255350-dmbl4emn 85 4 of of IN cord-255350-dmbl4emn 85 5 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 85 6 is be VBZ cord-255350-dmbl4emn 85 7 similar similar JJ cord-255350-dmbl4emn 85 8 to to IN cord-255350-dmbl4emn 85 9 those those DT cord-255350-dmbl4emn 85 10 of of IN cord-255350-dmbl4emn 85 11 the the DT cord-255350-dmbl4emn 85 12 other other JJ cord-255350-dmbl4emn 85 13 CEACAMs ceacam NNS cord-255350-dmbl4emn 85 14 that that WDT cord-255350-dmbl4emn 85 15 have have VBP cord-255350-dmbl4emn 85 16 been be VBN cord-255350-dmbl4emn 85 17 determined determine VBN cord-255350-dmbl4emn 85 18 previously previously RB cord-255350-dmbl4emn 85 19 . . . cord-255350-dmbl4emn 86 1 The the DT cord-255350-dmbl4emn 86 2 overall overall JJ cord-255350-dmbl4emn 86 3 topology topology NN cord-255350-dmbl4emn 86 4 is be VBZ cord-255350-dmbl4emn 86 5 that that DT cord-255350-dmbl4emn 86 6 of of IN cord-255350-dmbl4emn 86 7 the the DT cord-255350-dmbl4emn 86 8 V v NN cord-255350-dmbl4emn 86 9 - - HYPH cord-255350-dmbl4emn 86 10 set set NN cord-255350-dmbl4emn 86 11 fold fold NN cord-255350-dmbl4emn 86 12 of of IN cord-255350-dmbl4emn 86 13 the the DT cord-255350-dmbl4emn 86 14 immunoglobulin immunoglobulin NN cord-255350-dmbl4emn 86 15 superfamily superfamily NN cord-255350-dmbl4emn 86 16 , , , cord-255350-dmbl4emn 86 17 comprised comprise VBN cord-255350-dmbl4emn 86 18 of of IN cord-255350-dmbl4emn 86 19 two two CD cord-255350-dmbl4emn 86 20 -sheets -sheets HYPH cord-255350-dmbl4emn 86 21 labeled label VBN cord-255350-dmbl4emn 86 22 ABED ABED NNP cord-255350-dmbl4emn 86 23 and and CC cord-255350-dmbl4emn 86 24 A a DT cord-255350-dmbl4emn 86 25 0 0 CD cord-255350-dmbl4emn 86 26 GFCC gfcc NN cord-255350-dmbl4emn 86 27 0 0 CD cord-255350-dmbl4emn 86 28 C c NN cord-255350-dmbl4emn 86 29 00 00 NFP cord-255350-dmbl4emn 86 30 . . . cord-255350-dmbl4emn 87 1 The the DT cord-255350-dmbl4emn 87 2 sheets sheet NNS cord-255350-dmbl4emn 87 3 are be VBP cord-255350-dmbl4emn 87 4 connected connect VBN cord-255350-dmbl4emn 87 5 by by IN cord-255350-dmbl4emn 87 6 the the DT cord-255350-dmbl4emn 87 7 BC BC NNP cord-255350-dmbl4emn 87 8 , , , cord-255350-dmbl4emn 87 9 EF EF NNP cord-255350-dmbl4emn 87 10 , , , cord-255350-dmbl4emn 87 11 C c NN cord-255350-dmbl4emn 87 12 00 00 CD cord-255350-dmbl4emn 87 13 D d NN cord-255350-dmbl4emn 87 14 and and CC cord-255350-dmbl4emn 87 15 AA aa NN cord-255350-dmbl4emn 87 16 0 0 CD cord-255350-dmbl4emn 87 17 loops loop NNS cord-255350-dmbl4emn 87 18 ( ( -LRB- cord-255350-dmbl4emn 87 19 Fig fig NN cord-255350-dmbl4emn 87 20 . . . cord-255350-dmbl4emn 87 21 1b 1b CD cord-255350-dmbl4emn 87 22 ) ) -RRB- cord-255350-dmbl4emn 87 23 . . . cord-255350-dmbl4emn 88 1 The the DT cord-255350-dmbl4emn 88 2 r.m.s.d.s r.m.s.d. NNS cord-255350-dmbl4emn 88 3 of of IN cord-255350-dmbl4emn 88 4 the the DT cord-255350-dmbl4emn 88 5 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 88 6 molecules molecule NNS cord-255350-dmbl4emn 88 7 to to IN cord-255350-dmbl4emn 88 8 each each DT cord-255350-dmbl4emn 88 9 other other JJ cord-255350-dmbl4emn 88 10 are be VBP cord-255350-dmbl4emn 88 11 low low JJ cord-255350-dmbl4emn 88 12 ( ( -LRB- cord-255350-dmbl4emn 88 13 $ $ $ cord-255350-dmbl4emn 88 14 0.30 0.30 CD cord-255350-dmbl4emn 88 15 Å å NN cord-255350-dmbl4emn 88 16 ) ) -RRB- cord-255350-dmbl4emn 88 17 , , , cord-255350-dmbl4emn 88 18 showing show VBG cord-255350-dmbl4emn 88 19 no no DT cord-255350-dmbl4emn 88 20 structural structural JJ cord-255350-dmbl4emn 88 21 differences difference NNS cord-255350-dmbl4emn 88 22 within within IN cord-255350-dmbl4emn 88 23 the the DT cord-255350-dmbl4emn 88 24 asymmetric asymmetric JJ cord-255350-dmbl4emn 88 25 unit unit NN cord-255350-dmbl4emn 88 26 . . . cord-255350-dmbl4emn 89 1 The the DT cord-255350-dmbl4emn 89 2 four four CD cord-255350-dmbl4emn 89 3 molecules molecule NNS cord-255350-dmbl4emn 89 4 of of IN cord-255350-dmbl4emn 89 5 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 89 6 form form NN cord-255350-dmbl4emn 89 7 two two CD cord-255350-dmbl4emn 89 8 pairs pair NNS cord-255350-dmbl4emn 89 9 of of IN cord-255350-dmbl4emn 89 10 dimers dimer NNS cord-255350-dmbl4emn 89 11 ( ( -LRB- cord-255350-dmbl4emn 89 12 Fig Fig NNP cord-255350-dmbl4emn 89 13 . . . cord-255350-dmbl4emn 89 14 1c 1c CD cord-255350-dmbl4emn 89 15 ) ) -RRB- cord-255350-dmbl4emn 89 16 . . . cord-255350-dmbl4emn 90 1 The the DT cord-255350-dmbl4emn 90 2 dimer dimer NN cord-255350-dmbl4emn 90 3 interface interface NN cord-255350-dmbl4emn 90 4 is be VBZ cord-255350-dmbl4emn 90 5 formed form VBN cord-255350-dmbl4emn 90 6 from from IN cord-255350-dmbl4emn 90 7 the the DT cord-255350-dmbl4emn 90 8 second second JJ cord-255350-dmbl4emn 90 9 -sheet -sheet NN cord-255350-dmbl4emn 90 10 , , , cord-255350-dmbl4emn 90 11 A a DT cord-255350-dmbl4emn 90 12 0 0 CD cord-255350-dmbl4emn 90 13 GFCC gfcc NN cord-255350-dmbl4emn 90 14 0 0 CD cord-255350-dmbl4emn 90 15 C c NN cord-255350-dmbl4emn 90 16 00 00 NFP cord-255350-dmbl4emn 90 17 , , , cord-255350-dmbl4emn 90 18 specifically specifically RB cord-255350-dmbl4emn 90 19 the the DT cord-255350-dmbl4emn 90 20 GFCC GFCC NNP cord-255350-dmbl4emn 90 21 0 0 CD cord-255350-dmbl4emn 90 22 C c NN cord-255350-dmbl4emn 90 23 00 00 NFP cord-255350-dmbl4emn 90 24 strands strand NNS cord-255350-dmbl4emn 90 25 and and CC cord-255350-dmbl4emn 90 26 the the DT cord-255350-dmbl4emn 90 27 CC CC NNP cord-255350-dmbl4emn 90 28 0 0 CD cord-255350-dmbl4emn 90 29 , , , cord-255350-dmbl4emn 90 30 C c NN cord-255350-dmbl4emn 90 31 0 0 CD cord-255350-dmbl4emn 90 32 C c NN cord-255350-dmbl4emn 90 33 00 00 NFP cord-255350-dmbl4emn 90 34 and and CC cord-255350-dmbl4emn 90 35 FG FG NNP cord-255350-dmbl4emn 90 36 loops loop NNS cord-255350-dmbl4emn 90 37 ( ( -LRB- cord-255350-dmbl4emn 90 38 Fig Fig NNP cord-255350-dmbl4emn 90 39 . . . cord-255350-dmbl4emn 90 40 1c 1c CD cord-255350-dmbl4emn 90 41 ) ) -RRB- cord-255350-dmbl4emn 90 42 . . . cord-255350-dmbl4emn 91 1 Dimerization Dimerization NNP cord-255350-dmbl4emn 91 2 buries bury VBZ cord-255350-dmbl4emn 91 3 1610 1610 CD cord-255350-dmbl4emn 91 4 Å å NN cord-255350-dmbl4emn 91 5 2 2 CD cord-255350-dmbl4emn 91 6 of of IN cord-255350-dmbl4emn 91 7 solvent solvent NN cord-255350-dmbl4emn 91 8 - - HYPH cord-255350-dmbl4emn 91 9 accessible accessible JJ cord-255350-dmbl4emn 91 10 surface surface NN cord-255350-dmbl4emn 91 11 area area NN cord-255350-dmbl4emn 91 12 as as IN cord-255350-dmbl4emn 91 13 calculated calculate VBN cord-255350-dmbl4emn 91 14 by by IN cord-255350-dmbl4emn 91 15 PISA PISA NNP cord-255350-dmbl4emn 91 16 ( ( -LRB- cord-255350-dmbl4emn 91 17 Krissinel Krissinel NNP cord-255350-dmbl4emn 91 18 & & CC cord-255350-dmbl4emn 91 19 Henrick Henrick NNP cord-255350-dmbl4emn 91 20 , , , cord-255350-dmbl4emn 91 21 2007 2007 CD cord-255350-dmbl4emn 91 22 ) ) -RRB- cord-255350-dmbl4emn 91 23 . . . cord-255350-dmbl4emn 92 1 This this DT cord-255350-dmbl4emn 92 2 is be VBZ cord-255350-dmbl4emn 92 3 similar similar JJ cord-255350-dmbl4emn 92 4 to to IN cord-255350-dmbl4emn 92 5 the the DT cord-255350-dmbl4emn 92 6 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 92 7 and and CC cord-255350-dmbl4emn 92 8 CEACAM5 CEACAM5 NNP cord-255350-dmbl4emn 92 9 homodimers homodimer NNS cord-255350-dmbl4emn 92 10 , , , cord-255350-dmbl4emn 92 11 which which WDT cord-255350-dmbl4emn 92 12 bury bury VBP cord-255350-dmbl4emn 92 13 1600 1600 CD cord-255350-dmbl4emn 92 14 and and CC cord-255350-dmbl4emn 92 15 1460 1460 CD cord-255350-dmbl4emn 92 16 Å å NN cord-255350-dmbl4emn 92 17 2 2 CD cord-255350-dmbl4emn 92 18 of of IN cord-255350-dmbl4emn 92 19 solvent solvent NN cord-255350-dmbl4emn 92 20 - - HYPH cord-255350-dmbl4emn 92 21 accessible accessible JJ cord-255350-dmbl4emn 92 22 surface surface NN cord-255350-dmbl4emn 92 23 area area NN cord-255350-dmbl4emn 92 24 , , , cord-255350-dmbl4emn 92 25 respectively respectively RB cord-255350-dmbl4emn 92 26 . . . cord-255350-dmbl4emn 93 1 The the DT cord-255350-dmbl4emn 93 2 shape shape NN cord-255350-dmbl4emn 93 3 - - HYPH cord-255350-dmbl4emn 93 4 complementarity complementarity NN cord-255350-dmbl4emn 93 5 value value NN cord-255350-dmbl4emn 93 6 ( ( -LRB- cord-255350-dmbl4emn 93 7 Sc Sc NNP cord-255350-dmbl4emn 93 8 ; ; : cord-255350-dmbl4emn 93 9 Lawrence Lawrence NNP cord-255350-dmbl4emn 93 10 & & CC cord-255350-dmbl4emn 93 11 Colman Colman NNP cord-255350-dmbl4emn 93 12 , , , cord-255350-dmbl4emn 93 13 1993 1993 CD cord-255350-dmbl4emn 93 14 ) ) -RRB- cord-255350-dmbl4emn 93 15 is be VBZ cord-255350-dmbl4emn 93 16 0.68 0.68 CD cord-255350-dmbl4emn 93 17 , , , cord-255350-dmbl4emn 93 18 which which WDT cord-255350-dmbl4emn 93 19 is be VBZ cord-255350-dmbl4emn 93 20 smaller small JJR cord-255350-dmbl4emn 93 21 than than IN cord-255350-dmbl4emn 93 22 those those DT cord-255350-dmbl4emn 93 23 for for IN cord-255350-dmbl4emn 93 24 the the DT cord-255350-dmbl4emn 93 25 other other JJ cord-255350-dmbl4emn 93 26 CEACAMs ceacam NNS cord-255350-dmbl4emn 93 27 , , , cord-255350-dmbl4emn 93 28 with with IN cord-255350-dmbl4emn 93 29 values value NNS cord-255350-dmbl4emn 93 30 of of IN cord-255350-dmbl4emn 93 31 0.81 0.81 CD cord-255350-dmbl4emn 93 32 and and CC cord-255350-dmbl4emn 93 33 0.72 0.72 CD cord-255350-dmbl4emn 93 34 for for IN cord-255350-dmbl4emn 93 35 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 93 36 and and CC cord-255350-dmbl4emn 93 37 CEACAM5 CEACAM5 NNP cord-255350-dmbl4emn 93 38 , , , cord-255350-dmbl4emn 93 39 respectively respectively RB cord-255350-dmbl4emn 93 40 . . . cord-255350-dmbl4emn 94 1 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 95 1 forms form VBZ cord-255350-dmbl4emn 95 2 nine nine CD cord-255350-dmbl4emn 95 3 hydrogen hydrogen NN cord-255350-dmbl4emn 95 4 bonds bond NNS cord-255350-dmbl4emn 95 5 in in IN cord-255350-dmbl4emn 95 6 the the DT cord-255350-dmbl4emn 95 7 dimerization dimerization NN cord-255350-dmbl4emn 95 8 interface interface NN cord-255350-dmbl4emn 95 9 . . . cord-255350-dmbl4emn 96 1 This this DT cord-255350-dmbl4emn 96 2 is be VBZ cord-255350-dmbl4emn 96 3 more more JJR cord-255350-dmbl4emn 96 4 than than IN cord-255350-dmbl4emn 96 5 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 97 1 ( ( -LRB- cord-255350-dmbl4emn 97 2 six six CD cord-255350-dmbl4emn 97 3 hydrogen hydrogen NN cord-255350-dmbl4emn 97 4 bonds bond NNS cord-255350-dmbl4emn 97 5 ) ) -RRB- cord-255350-dmbl4emn 97 6 but but CC cord-255350-dmbl4emn 97 7 less less JJR cord-255350-dmbl4emn 97 8 than than IN cord-255350-dmbl4emn 97 9 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 98 1 ( ( -LRB- cord-255350-dmbl4emn 98 2 16 16 CD cord-255350-dmbl4emn 98 3 hydrogen hydrogen NN cord-255350-dmbl4emn 98 4 bonds bond NNS cord-255350-dmbl4emn 98 5 ) ) -RRB- cord-255350-dmbl4emn 98 6 . . . cord-255350-dmbl4emn 99 1 Of of IN cord-255350-dmbl4emn 99 2 the the DT cord-255350-dmbl4emn 99 3 38 38 CD cord-255350-dmbl4emn 99 4 residues residue NNS cord-255350-dmbl4emn 99 5 in in IN cord-255350-dmbl4emn 99 6 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 99 7 that that WDT cord-255350-dmbl4emn 99 8 differ differ VBP cord-255350-dmbl4emn 99 9 from from IN cord-255350-dmbl4emn 99 10 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 100 1 and and CC cord-255350-dmbl4emn 100 2 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 100 3 , , , cord-255350-dmbl4emn 100 4 eight eight CD cord-255350-dmbl4emn 100 5 are be VBP cord-255350-dmbl4emn 100 6 found find VBN cord-255350-dmbl4emn 100 7 in in IN cord-255350-dmbl4emn 100 8 the the DT cord-255350-dmbl4emn 100 9 dimerization dimerization NN cord-255350-dmbl4emn 100 10 interface interface NN cord-255350-dmbl4emn 100 11 . . . cord-255350-dmbl4emn 101 1 The the DT cord-255350-dmbl4emn 101 2 dimerization dimerization NN cord-255350-dmbl4emn 101 3 constant constant JJ cord-255350-dmbl4emn 101 4 of of IN cord-255350-dmbl4emn 101 5 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 101 6 was be VBD cord-255350-dmbl4emn 101 7 determined determine VBN cord-255350-dmbl4emn 101 8 previously previously RB cord-255350-dmbl4emn 101 9 to to TO cord-255350-dmbl4emn 101 10 be be VB cord-255350-dmbl4emn 101 11 0.8 0.8 CD cord-255350-dmbl4emn 101 12 mM mm CD cord-255350-dmbl4emn 101 13 by by IN cord-255350-dmbl4emn 101 14 analytical analytical JJ cord-255350-dmbl4emn 101 15 ultracentrifugation ultracentrifugation NN cord-255350-dmbl4emn 102 1 ( ( -LRB- cord-255350-dmbl4emn 102 2 Korotkova Korotkova NNP cord-255350-dmbl4emn 102 3 et et FW cord-255350-dmbl4emn 102 4 al al NNP cord-255350-dmbl4emn 102 5 . . NNP cord-255350-dmbl4emn 102 6 , , , cord-255350-dmbl4emn 102 7 2008 2008 CD cord-255350-dmbl4emn 102 8 ) ) -RRB- cord-255350-dmbl4emn 102 9 . . . cord-255350-dmbl4emn 103 1 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 103 2 does do VBZ cord-255350-dmbl4emn 103 3 dimerize dimerize NNP cord-255350-dmbl4emn 103 4 but but CC cord-255350-dmbl4emn 103 5 forms form VBZ cord-255350-dmbl4emn 103 6 high high JJ cord-255350-dmbl4emn 103 7 molecular molecular JJ cord-255350-dmbl4emn 103 8 - - HYPH cord-255350-dmbl4emn 103 9 weight weight NN cord-255350-dmbl4emn 103 10 oligomers oligomer NNS cord-255350-dmbl4emn 103 11 ( ( -LRB- cord-255350-dmbl4emn 103 12 Korotkova Korotkova NNP cord-255350-dmbl4emn 103 13 et et FW cord-255350-dmbl4emn 103 14 al al NNP cord-255350-dmbl4emn 103 15 . . NNP cord-255350-dmbl4emn 103 16 , , , cord-255350-dmbl4emn 103 17 2008 2008 CD cord-255350-dmbl4emn 103 18 ) ) -RRB- cord-255350-dmbl4emn 103 19 . . . cord-255350-dmbl4emn 104 1 The the DT cord-255350-dmbl4emn 104 2 dimerization dimerization NN cord-255350-dmbl4emn 104 3 constant constant NN cord-255350-dmbl4emn 104 4 of of IN cord-255350-dmbl4emn 104 5 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 104 6 was be VBD cord-255350-dmbl4emn 104 7 estimated estimate VBN cord-255350-dmbl4emn 104 8 by by IN cord-255350-dmbl4emn 104 9 sedimentation sedimentation NN cord-255350-dmbl4emn 104 10 - - HYPH cord-255350-dmbl4emn 104 11 equilibrium equilibrium NN cord-255350-dmbl4emn 104 12 analysis analysis NN cord-255350-dmbl4emn 104 13 using use VBG cord-255350-dmbl4emn 104 14 analytical analytical JJ cord-255350-dmbl4emn 104 15 ultra- ultra- NN cord-255350-dmbl4emn 104 16 in in IN cord-255350-dmbl4emn 104 17 50 50 CD cord-255350-dmbl4emn 104 18 mM mm CD cord-255350-dmbl4emn 104 19 Tris Tris NNP cord-255350-dmbl4emn 104 20 - - HYPH cord-255350-dmbl4emn 104 21 HCl HCl NNP cord-255350-dmbl4emn 104 22 , , , cord-255350-dmbl4emn 104 23 50 50 CD cord-255350-dmbl4emn 104 24 mM mm CD cord-255350-dmbl4emn 104 25 sodium sodium NN cord-255350-dmbl4emn 104 26 chloride chloride NN cord-255350-dmbl4emn 104 27 pH pH NNP cord-255350-dmbl4emn 104 28 7.5 7.5 CD cord-255350-dmbl4emn 104 29 with with IN cord-255350-dmbl4emn 104 30 rotor rotor NN cord-255350-dmbl4emn 104 31 speeds speed NNS cord-255350-dmbl4emn 104 32 of of IN cord-255350-dmbl4emn 104 33 29 29 CD cord-255350-dmbl4emn 104 34 000 000 CD cord-255350-dmbl4emn 104 35 , , , cord-255350-dmbl4emn 104 36 32 32 CD cord-255350-dmbl4emn 104 37 000 000 CD cord-255350-dmbl4emn 104 38 and and CC cord-255350-dmbl4emn 104 39 35 35 CD cord-255350-dmbl4emn 104 40 000 000 CD cord-255350-dmbl4emn 104 41 rev rev NN cord-255350-dmbl4emn 104 42 min min NNP cord-255350-dmbl4emn 104 43 À1 À1 NNP cord-255350-dmbl4emn 104 44 ( ( -LRB- cord-255350-dmbl4emn 104 45 blue blue JJ cord-255350-dmbl4emn 104 46 , , , cord-255350-dmbl4emn 104 47 red red JJ cord-255350-dmbl4emn 104 48 and and CC cord-255350-dmbl4emn 104 49 green green JJ cord-255350-dmbl4emn 104 50 curves curve NNS cord-255350-dmbl4emn 104 51 , , , cord-255350-dmbl4emn 104 52 respectively respectively RB cord-255350-dmbl4emn 104 53 ) ) -RRB- cord-255350-dmbl4emn 104 54 . . . cord-255350-dmbl4emn 105 1 Bottom bottom RB cord-255350-dmbl4emn 105 2 , , , cord-255350-dmbl4emn 105 3 residuals residual NNS cord-255350-dmbl4emn 105 4 of of IN cord-255350-dmbl4emn 105 5 fitted fit VBN cord-255350-dmbl4emn 105 6 data datum NNS cord-255350-dmbl4emn 105 7 for for IN cord-255350-dmbl4emn 105 8 each each DT cord-255350-dmbl4emn 105 9 curve curve NN cord-255350-dmbl4emn 105 10 . . . cord-255350-dmbl4emn 106 1 centrifugation centrifugation NN cord-255350-dmbl4emn 106 2 ( ( -LRB- cord-255350-dmbl4emn 106 3 Fig Fig NNP cord-255350-dmbl4emn 106 4 . . . cord-255350-dmbl4emn 106 5 1d 1d CD cord-255350-dmbl4emn 106 6 ) ) -RRB- cord-255350-dmbl4emn 106 7 . . . cord-255350-dmbl4emn 107 1 An an DT cord-255350-dmbl4emn 107 2 estimated estimated JJ cord-255350-dmbl4emn 107 3 average average JJ cord-255350-dmbl4emn 107 4 molecular molecular JJ cord-255350-dmbl4emn 107 5 weight weight NN cord-255350-dmbl4emn 107 6 of of IN cord-255350-dmbl4emn 107 7 24.7 24.7 CD cord-255350-dmbl4emn 107 8 AE AE NNP cord-255350-dmbl4emn 107 9 0.7 0.7 CD cord-255350-dmbl4emn 107 10 kDa kDa NNS cord-255350-dmbl4emn 107 11 ( ( -LRB- cord-255350-dmbl4emn 107 12 the the DT cord-255350-dmbl4emn 107 13 theoretical theoretical JJ cord-255350-dmbl4emn 107 14 monomer monomer NN cord-255350-dmbl4emn 107 15 molecular molecular JJ cord-255350-dmbl4emn 107 16 weight weight NN cord-255350-dmbl4emn 107 17 is be VBZ cord-255350-dmbl4emn 107 18 12 12 CD cord-255350-dmbl4emn 107 19 574 574 CD cord-255350-dmbl4emn 107 20 Da Da NNS cord-255350-dmbl4emn 107 21 ) ) -RRB- cord-255350-dmbl4emn 107 22 and and CC cord-255350-dmbl4emn 108 1 a a DT cord-255350-dmbl4emn 108 2 K K NNP cord-255350-dmbl4emn 108 3 dimerization dimerization NN cord-255350-dmbl4emn 108 4 of of IN cord-255350-dmbl4emn 108 5 95 95 CD cord-255350-dmbl4emn 108 6 nM nm NN cord-255350-dmbl4emn 108 7 ( ( -LRB- cord-255350-dmbl4emn 108 8 +20 +20 CD cord-255350-dmbl4emn 108 9 / / SYM cord-255350-dmbl4emn 108 10 À60 À60 NNP cord-255350-dmbl4emn 108 11 nM nM NNP cord-255350-dmbl4emn 108 12 ) ) -RRB- cord-255350-dmbl4emn 108 13 were be VBD cord-255350-dmbl4emn 108 14 measured measure VBN cord-255350-dmbl4emn 108 15 , , , cord-255350-dmbl4emn 108 16 a a DT cord-255350-dmbl4emn 108 17 tenfold tenfold JJ cord-255350-dmbl4emn 108 18 increase increase NN cord-255350-dmbl4emn 108 19 in in IN cord-255350-dmbl4emn 108 20 affinity affinity NN cord-255350-dmbl4emn 108 21 when when WRB cord-255350-dmbl4emn 108 22 compared compare VBN cord-255350-dmbl4emn 108 23 with with IN cord-255350-dmbl4emn 108 24 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 108 25 . . . cord-255350-dmbl4emn 109 1 We -PRON- PRP cord-255350-dmbl4emn 109 2 observed observe VBD cord-255350-dmbl4emn 109 3 no no DT cord-255350-dmbl4emn 109 4 higher high JJR cord-255350-dmbl4emn 109 5 molecular molecular JJ cord-255350-dmbl4emn 109 6 weight weight NN cord-255350-dmbl4emn 109 7 oligomers oligomer NNS cord-255350-dmbl4emn 109 8 for for IN cord-255350-dmbl4emn 109 9 CEACAM7 ceacam7 XX cord-255350-dmbl4emn 109 10 other other JJ cord-255350-dmbl4emn 109 11 than than IN cord-255350-dmbl4emn 109 12 the the DT cord-255350-dmbl4emn 109 13 dimer dimer NN cord-255350-dmbl4emn 109 14 . . . cord-255350-dmbl4emn 110 1 Superposition superposition NN cord-255350-dmbl4emn 110 2 of of IN cord-255350-dmbl4emn 110 3 the the DT cord-255350-dmbl4emn 110 4 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 110 5 dimer dimer NN cord-255350-dmbl4emn 110 6 ( ( -LRB- cord-255350-dmbl4emn 110 7 A a NN cord-255350-dmbl4emn 110 8 + + CC cord-255350-dmbl4emn 110 9 B b NN cord-255350-dmbl4emn 110 10 ) ) -RRB- cord-255350-dmbl4emn 110 11 onto onto IN cord-255350-dmbl4emn 110 12 the the DT cord-255350-dmbl4emn 110 13 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 110 14 dimer dimer NN cord-255350-dmbl4emn 110 15 ( ( -LRB- cord-255350-dmbl4emn 110 16 A a NN cord-255350-dmbl4emn 110 17 + + CC cord-255350-dmbl4emn 110 18 B b NN cord-255350-dmbl4emn 110 19 ) ) -RRB- cord-255350-dmbl4emn 110 20 was be VBD cord-255350-dmbl4emn 110 21 achieved achieve VBN cord-255350-dmbl4emn 110 22 using use VBG cord-255350-dmbl4emn 110 23 one one CD cord-255350-dmbl4emn 110 24 half half NN cord-255350-dmbl4emn 110 25 of of IN cord-255350-dmbl4emn 110 26 each each DT cord-255350-dmbl4emn 110 27 dimer dimer NN cord-255350-dmbl4emn 110 28 ( ( -LRB- cord-255350-dmbl4emn 110 29 molecule molecule NN cord-255350-dmbl4emn 110 30 A A NNP cord-255350-dmbl4emn 110 31 ) ) -RRB- cord-255350-dmbl4emn 110 32 and and CC cord-255350-dmbl4emn 110 33 two two CD cord-255350-dmbl4emn 110 34 r.m.s.d.s r.m.s.d. NNS cord-255350-dmbl4emn 110 35 were be VBD cord-255350-dmbl4emn 110 36 calculated calculate VBN cord-255350-dmbl4emn 110 37 : : : cord-255350-dmbl4emn 110 38 one one CD cord-255350-dmbl4emn 110 39 for for IN cord-255350-dmbl4emn 110 40 the the DT cord-255350-dmbl4emn 110 41 first first JJ cord-255350-dmbl4emn 110 42 half half NN cord-255350-dmbl4emn 110 43 of of IN cord-255350-dmbl4emn 110 44 the the DT cord-255350-dmbl4emn 110 45 dimer dimer NN cord-255350-dmbl4emn 110 46 ( ( -LRB- cord-255350-dmbl4emn 110 47 molecule molecule NN cord-255350-dmbl4emn 110 48 A A NNP cord-255350-dmbl4emn 110 49 ) ) -RRB- cord-255350-dmbl4emn 110 50 , , , cord-255350-dmbl4emn 110 51 which which WDT cord-255350-dmbl4emn 110 52 results result VBZ cord-255350-dmbl4emn 110 53 in in IN cord-255350-dmbl4emn 110 54 an an DT cord-255350-dmbl4emn 110 55 r.m.s.d r.m.s.d NNP cord-255350-dmbl4emn 110 56 . . NNP cord-255350-dmbl4emn 110 57 of of IN cord-255350-dmbl4emn 110 58 0.67 0.67 CD cord-255350-dmbl4emn 110 59 Å å NN cord-255350-dmbl4emn 110 60 , , , cord-255350-dmbl4emn 110 61 and and CC cord-255350-dmbl4emn 110 62 the the DT cord-255350-dmbl4emn 110 63 second second JJ cord-255350-dmbl4emn 110 64 for for IN cord-255350-dmbl4emn 110 65 the the DT cord-255350-dmbl4emn 110 66 second second JJ cord-255350-dmbl4emn 110 67 half half NN cord-255350-dmbl4emn 110 68 , , , cord-255350-dmbl4emn 110 69 which which WDT cord-255350-dmbl4emn 110 70 shows show VBZ cord-255350-dmbl4emn 110 71 a a DT cord-255350-dmbl4emn 110 72 larger large JJR cord-255350-dmbl4emn 110 73 r.m.s.d r.m.s.d NNP cord-255350-dmbl4emn 110 74 . . . cord-255350-dmbl4emn 111 1 of of IN cord-255350-dmbl4emn 111 2 2.70 2.70 CD cord-255350-dmbl4emn 111 3 Å å NN cord-255350-dmbl4emn 111 4 ( ( -LRB- cord-255350-dmbl4emn 111 5 molecule molecule NN cord-255350-dmbl4emn 111 6 B B NNP cord-255350-dmbl4emn 111 7 ; ; : cord-255350-dmbl4emn 111 8 Fig Fig NNP cord-255350-dmbl4emn 111 9 . . . cord-255350-dmbl4emn 111 10 2a 2a CD cord-255350-dmbl4emn 111 11 ) ) -RRB- cord-255350-dmbl4emn 111 12 . . . cord-255350-dmbl4emn 112 1 Superposition superposition NN cord-255350-dmbl4emn 112 2 of of IN cord-255350-dmbl4emn 112 3 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 112 4 onto onto IN cord-255350-dmbl4emn 112 5 the the DT cord-255350-dmbl4emn 112 6 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 112 7 dimer dimer NN cord-255350-dmbl4emn 112 8 using use VBG cord-255350-dmbl4emn 112 9 the the DT cord-255350-dmbl4emn 112 10 same same JJ cord-255350-dmbl4emn 112 11 method method NN cord-255350-dmbl4emn 112 12 reveals reveal VBZ cord-255350-dmbl4emn 112 13 similar similar JJ cord-255350-dmbl4emn 112 14 r.m.s.d.s r.m.s.d. NNS cord-255350-dmbl4emn 112 15 of of IN cord-255350-dmbl4emn 112 16 0.83 0.83 CD cord-255350-dmbl4emn 112 17 Å å NN cord-255350-dmbl4emn 112 18 for for IN cord-255350-dmbl4emn 112 19 molecule molecule NN cord-255350-dmbl4emn 112 20 A a NN cord-255350-dmbl4emn 112 21 and and CC cord-255350-dmbl4emn 112 22 2.26 2.26 CD cord-255350-dmbl4emn 112 23 Å å NN cord-255350-dmbl4emn 112 24 for for IN cord-255350-dmbl4emn 112 25 molecule molecule NN cord-255350-dmbl4emn 112 26 B B NNP cord-255350-dmbl4emn 112 27 ( ( -LRB- cord-255350-dmbl4emn 112 28 Fig Fig NNP cord-255350-dmbl4emn 112 29 . . . cord-255350-dmbl4emn 112 30 2b 2b NNP cord-255350-dmbl4emn 112 31 ) ) -RRB- cord-255350-dmbl4emn 112 32 . . . cord-255350-dmbl4emn 113 1 Closer close JJR cord-255350-dmbl4emn 113 2 comparison comparison NN cord-255350-dmbl4emn 113 3 of of IN cord-255350-dmbl4emn 113 4 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 113 5 with with IN cord-255350-dmbl4emn 113 6 CEACAM5 CEACAM5 NNP cord-255350-dmbl4emn 114 1 and and CC cord-255350-dmbl4emn 114 2 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 114 3 shows show VBZ cord-255350-dmbl4emn 114 4 two two CD cord-255350-dmbl4emn 114 5 major major JJ cord-255350-dmbl4emn 114 6 regions region NNS cord-255350-dmbl4emn 114 7 of of IN cord-255350-dmbl4emn 114 8 deviation deviation NN cord-255350-dmbl4emn 114 9 . . . cord-255350-dmbl4emn 115 1 The the DT cord-255350-dmbl4emn 115 2 first first JJ cord-255350-dmbl4emn 115 3 is be VBZ cord-255350-dmbl4emn 115 4 the the DT cord-255350-dmbl4emn 115 5 BC BC NNP cord-255350-dmbl4emn 115 6 loop loop NN cord-255350-dmbl4emn 115 7 ( ( -LRB- cord-255350-dmbl4emn 115 8 residues residue NNS cord-255350-dmbl4emn 115 9 23 23 CD cord-255350-dmbl4emn 115 10 - - SYM cord-255350-dmbl4emn 115 11 29 29 CD cord-255350-dmbl4emn 115 12 ) ) -RRB- cord-255350-dmbl4emn 115 13 , , , cord-255350-dmbl4emn 115 14 which which WDT cord-255350-dmbl4emn 115 15 is be VBZ cord-255350-dmbl4emn 115 16 not not RB cord-255350-dmbl4emn 115 17 involved involve VBN cord-255350-dmbl4emn 115 18 in in IN cord-255350-dmbl4emn 115 19 the the DT cord-255350-dmbl4emn 115 20 dimerization dimerization NN cord-255350-dmbl4emn 115 21 interface interface NN cord-255350-dmbl4emn 115 22 ( ( -LRB- cord-255350-dmbl4emn 115 23 Fig Fig NNP cord-255350-dmbl4emn 115 24 . . . cord-255350-dmbl4emn 115 25 2c 2c NNP cord-255350-dmbl4emn 115 26 ) ) -RRB- cord-255350-dmbl4emn 115 27 . . . cord-255350-dmbl4emn 116 1 This this DT cord-255350-dmbl4emn 116 2 region region NN cord-255350-dmbl4emn 116 3 is be VBZ cord-255350-dmbl4emn 116 4 highly highly RB cord-255350-dmbl4emn 116 5 conserved conserve VBN cord-255350-dmbl4emn 116 6 among among IN cord-255350-dmbl4emn 116 7 members member NNS cord-255350-dmbl4emn 116 8 of of IN cord-255350-dmbl4emn 116 9 the the DT cord-255350-dmbl4emn 116 10 CEACAM CEACAM NNP cord-255350-dmbl4emn 116 11 family family NN cord-255350-dmbl4emn 116 12 ; ; : cord-255350-dmbl4emn 116 13 however however RB cord-255350-dmbl4emn 116 14 , , , cord-255350-dmbl4emn 116 15 positions position VBZ cord-255350-dmbl4emn 116 16 25 25 CD cord-255350-dmbl4emn 116 17 - - SYM cord-255350-dmbl4emn 116 18 26 26 CD cord-255350-dmbl4emn 116 19 of of IN cord-255350-dmbl4emn 116 20 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 116 21 differ differ VBP cord-255350-dmbl4emn 116 22 from from IN cord-255350-dmbl4emn 116 23 those those DT cord-255350-dmbl4emn 116 24 of of IN cord-255350-dmbl4emn 116 25 the the DT cord-255350-dmbl4emn 116 26 other other JJ cord-255350-dmbl4emn 116 27 CEACAMs ceacam NNS cord-255350-dmbl4emn 116 28 . . . cord-255350-dmbl4emn 117 1 In in IN cord-255350-dmbl4emn 117 2 all all DT cord-255350-dmbl4emn 117 3 other other JJ cord-255350-dmbl4emn 117 4 CEACAMs ceacam NNS cord-255350-dmbl4emn 117 5 these these DT cord-255350-dmbl4emn 117 6 residues residue NNS cord-255350-dmbl4emn 117 7 are be VBP cord-255350-dmbl4emn 117 8 Leu25 Leu25 NNP cord-255350-dmbl4emn 117 9 and and CC cord-255350-dmbl4emn 117 10 Pro26 Pro26 NNP cord-255350-dmbl4emn 117 11 . . . cord-255350-dmbl4emn 118 1 However however RB cord-255350-dmbl4emn 118 2 , , , cord-255350-dmbl4emn 118 3 in in IN cord-255350-dmbl4emn 118 4 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 118 5 they -PRON- PRP cord-255350-dmbl4emn 118 6 are be VBP cord-255350-dmbl4emn 118 7 Glu25 Glu25 NNP cord-255350-dmbl4emn 118 8 and and CC cord-255350-dmbl4emn 118 9 Ser26 Ser26 NNP cord-255350-dmbl4emn 118 10 . . . cord-255350-dmbl4emn 119 1 The the DT cord-255350-dmbl4emn 119 2 loss loss NN cord-255350-dmbl4emn 119 3 of of IN cord-255350-dmbl4emn 119 4 Pro26 Pro26 NNP cord-255350-dmbl4emn 119 5 is be VBZ cord-255350-dmbl4emn 119 6 likely likely JJ cord-255350-dmbl4emn 119 7 to to TO cord-255350-dmbl4emn 119 8 reduce reduce VB cord-255350-dmbl4emn 119 9 the the DT cord-255350-dmbl4emn 119 10 rigidity rigidity NN cord-255350-dmbl4emn 119 11 of of IN cord-255350-dmbl4emn 119 12 the the DT cord-255350-dmbl4emn 119 13 loop loop NN cord-255350-dmbl4emn 119 14 . . . cord-255350-dmbl4emn 120 1 The the DT cord-255350-dmbl4emn 120 2 r.m.s.d r.m.s.d NNP cord-255350-dmbl4emn 120 3 . . . cord-255350-dmbl4emn 120 4 of of IN cord-255350-dmbl4emn 120 5 this this DT cord-255350-dmbl4emn 120 6 loop loop NN cord-255350-dmbl4emn 120 7 in in IN cord-255350-dmbl4emn 120 8 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 120 9 is be VBZ cord-255350-dmbl4emn 120 10 3.10 3.10 CD cord-255350-dmbl4emn 120 11 Å å NN cord-255350-dmbl4emn 120 12 relative relative JJ cord-255350-dmbl4emn 120 13 to to IN cord-255350-dmbl4emn 120 14 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 120 15 and and CC cord-255350-dmbl4emn 121 1 the the DT cord-255350-dmbl4emn 121 2 displacement displacement NN cord-255350-dmbl4emn 121 3 of of IN cord-255350-dmbl4emn 121 4 this this DT cord-255350-dmbl4emn 121 5 loop loop NN cord-255350-dmbl4emn 121 6 causes cause VBZ cord-255350-dmbl4emn 121 7 a a DT cord-255350-dmbl4emn 121 8 slight slight JJ cord-255350-dmbl4emn 121 9 movement movement NN cord-255350-dmbl4emn 121 10 of of IN cord-255350-dmbl4emn 121 11 the the DT cord-255350-dmbl4emn 121 12 N n CD cord-255350-dmbl4emn 121 13 - - HYPH cord-255350-dmbl4emn 121 14 terminal terminal NN cord-255350-dmbl4emn 121 15 -strand -strand CD cord-255350-dmbl4emn 121 16 . . . cord-255350-dmbl4emn 122 1 Notably notably RB cord-255350-dmbl4emn 122 2 , , , cord-255350-dmbl4emn 122 3 the the DT cord-255350-dmbl4emn 122 4 preceding precede VBG cord-255350-dmbl4emn 122 5 residue residue NN cord-255350-dmbl4emn 122 6 is be VBZ cord-255350-dmbl4emn 122 7 Asn24 Asn24 NNP cord-255350-dmbl4emn 122 8 , , , cord-255350-dmbl4emn 122 9 a a DT cord-255350-dmbl4emn 122 10 unique unique JJ cord-255350-dmbl4emn 122 11 N n NN cord-255350-dmbl4emn 122 12 - - HYPH cord-255350-dmbl4emn 122 13 linked link VBN cord-255350-dmbl4emn 122 14 glycosylation glycosylation NN cord-255350-dmbl4emn 122 15 site site NN cord-255350-dmbl4emn 122 16 found find VBN cord-255350-dmbl4emn 122 17 only only RB cord-255350-dmbl4emn 122 18 in in IN cord-255350-dmbl4emn 122 19 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 122 20 and and CC cord-255350-dmbl4emn 122 21 CEACAM4 CEACAM4 NNP cord-255350-dmbl4emn 122 22 . . . cord-255350-dmbl4emn 123 1 Three three CD cord-255350-dmbl4emn 123 2 other other JJ cord-255350-dmbl4emn 123 3 glycosylation glycosylation NN cord-255350-dmbl4emn 123 4 sites site NNS cord-255350-dmbl4emn 123 5 are be VBP cord-255350-dmbl4emn 123 6 present present JJ cord-255350-dmbl4emn 123 7 in in IN cord-255350-dmbl4emn 123 8 CEACAM7 ceacam7 XX cord-255350-dmbl4emn 124 1 ( ( -LRB- cord-255350-dmbl4emn 124 2 Asn52 Asn52 NNP cord-255350-dmbl4emn 124 3 , , , cord-255350-dmbl4emn 124 4 Asn72 Asn72 NNP cord-255350-dmbl4emn 124 5 and and CC cord-255350-dmbl4emn 124 6 Asn79 Asn79 NNP cord-255350-dmbl4emn 124 7 ) ) -RRB- cord-255350-dmbl4emn 124 8 . . . cord-255350-dmbl4emn 125 1 These these DT cord-255350-dmbl4emn 125 2 are be VBP cord-255350-dmbl4emn 125 3 highlighted highlight VBN cord-255350-dmbl4emn 125 4 in in IN cord-255350-dmbl4emn 125 5 Fig Fig NNP cord-255350-dmbl4emn 125 6 . . . cord-255350-dmbl4emn 125 7 2(d 2(d CD cord-255350-dmbl4emn 125 8 ) ) -RRB- cord-255350-dmbl4emn 125 9 , , , cord-255350-dmbl4emn 125 10 showing show VBG cord-255350-dmbl4emn 125 11 that that IN cord-255350-dmbl4emn 125 12 none none NN cord-255350-dmbl4emn 125 13 are be VBP cord-255350-dmbl4emn 125 14 found find VBN cord-255350-dmbl4emn 125 15 in in IN cord-255350-dmbl4emn 125 16 the the DT cord-255350-dmbl4emn 125 17 dimerization dimerization NN cord-255350-dmbl4emn 125 18 interface interface NN cord-255350-dmbl4emn 125 19 . . . cord-255350-dmbl4emn 126 1 Glycosylation glycosylation NN cord-255350-dmbl4emn 126 2 of of IN cord-255350-dmbl4emn 126 3 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 126 4 has have VBZ cord-255350-dmbl4emn 126 5 been be VBN cord-255350-dmbl4emn 126 6 shown show VBN cord-255350-dmbl4emn 126 7 to to TO cord-255350-dmbl4emn 126 8 be be VB cord-255350-dmbl4emn 126 9 important important JJ cord-255350-dmbl4emn 126 10 for for IN cord-255350-dmbl4emn 126 11 interaction interaction NN cord-255350-dmbl4emn 126 12 with with IN cord-255350-dmbl4emn 126 13 CD8 CD8 NNP cord-255350-dmbl4emn 126 14 ( ( -LRB- cord-255350-dmbl4emn 126 15 Roda Roda NNP cord-255350-dmbl4emn 126 16 et et NNP cord-255350-dmbl4emn 126 17 al al NNP cord-255350-dmbl4emn 126 18 . . NNP cord-255350-dmbl4emn 126 19 , , , cord-255350-dmbl4emn 126 20 2014 2014 CD cord-255350-dmbl4emn 126 21 ) ) -RRB- cord-255350-dmbl4emn 126 22 and and CC cord-255350-dmbl4emn 126 23 therefore therefore RB cord-255350-dmbl4emn 126 24 may may MD cord-255350-dmbl4emn 126 25 also also RB cord-255350-dmbl4emn 126 26 be be VB cord-255350-dmbl4emn 126 27 important important JJ cord-255350-dmbl4emn 126 28 for for IN cord-255350-dmbl4emn 126 29 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 126 30 function function NN cord-255350-dmbl4emn 126 31 . . . cord-255350-dmbl4emn 127 1 The the DT cord-255350-dmbl4emn 127 2 second second JJ cord-255350-dmbl4emn 127 3 region region NN cord-255350-dmbl4emn 127 4 of of IN cord-255350-dmbl4emn 127 5 deviation deviation NN cord-255350-dmbl4emn 127 6 between between IN cord-255350-dmbl4emn 127 7 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 127 8 , , , cord-255350-dmbl4emn 127 9 CEACAM5 CEACAM5 '' cord-255350-dmbl4emn 127 10 and and CC cord-255350-dmbl4emn 127 11 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 127 12 is be VBZ cord-255350-dmbl4emn 127 13 the the DT cord-255350-dmbl4emn 127 14 C c NN cord-255350-dmbl4emn 127 15 0 0 CD cord-255350-dmbl4emn 127 16 C c NN cord-255350-dmbl4emn 127 17 00 00 CD cord-255350-dmbl4emn 127 18 loop loop NN cord-255350-dmbl4emn 127 19 and and CC cord-255350-dmbl4emn 127 20 the the DT cord-255350-dmbl4emn 127 21 C C NNP cord-255350-dmbl4emn 127 22 00 00 CD cord-255350-dmbl4emn 127 23 strand strand NN cord-255350-dmbl4emn 127 24 . . . cord-255350-dmbl4emn 128 1 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 128 2 is be VBZ cord-255350-dmbl4emn 128 3 unique unique JJ cord-255350-dmbl4emn 128 4 compared compare VBN cord-255350-dmbl4emn 128 5 with with IN cord-255350-dmbl4emn 128 6 other other JJ cord-255350-dmbl4emn 128 7 CEACAMs ceacam NNS cord-255350-dmbl4emn 128 8 , , , cord-255350-dmbl4emn 128 9 as as IN cord-255350-dmbl4emn 128 10 the the DT cord-255350-dmbl4emn 128 11 C c NN cord-255350-dmbl4emn 128 12 0 0 CD cord-255350-dmbl4emn 128 13 C c NN cord-255350-dmbl4emn 128 14 00 00 CD cord-255350-dmbl4emn 128 15 loop loop NN cord-255350-dmbl4emn 128 16 contains contain VBZ cord-255350-dmbl4emn 128 17 a a DT cord-255350-dmbl4emn 128 18 single single JJ cord-255350-dmbl4emn 128 19 amino amino NN cord-255350-dmbl4emn 128 20 - - HYPH cord-255350-dmbl4emn 128 21 acid acid NN cord-255350-dmbl4emn 128 22 insertion insertion NN cord-255350-dmbl4emn 128 23 ( ( -LRB- cord-255350-dmbl4emn 128 24 isoleucine isoleucine NNP cord-255350-dmbl4emn 128 25 ) ) -RRB- cord-255350-dmbl4emn 128 26 between between IN cord-255350-dmbl4emn 128 27 residues residue NNS cord-255350-dmbl4emn 128 28 52 52 CD cord-255350-dmbl4emn 128 29 and and CC cord-255350-dmbl4emn 128 30 53 53 CD cord-255350-dmbl4emn 128 31 . . . cord-255350-dmbl4emn 129 1 To to TO cord-255350-dmbl4emn 129 2 accommodate accommodate VB cord-255350-dmbl4emn 129 3 the the DT cord-255350-dmbl4emn 129 4 insertion insertion NN cord-255350-dmbl4emn 129 5 in in IN cord-255350-dmbl4emn 129 6 the the DT cord-255350-dmbl4emn 129 7 C c NN cord-255350-dmbl4emn 129 8 0 0 CD cord-255350-dmbl4emn 129 9 C c NN cord-255350-dmbl4emn 129 10 00 00 NFP cord-255350-dmbl4emn 129 11 loop loop NN cord-255350-dmbl4emn 129 12 without without IN cord-255350-dmbl4emn 129 13 altering alter VBG cord-255350-dmbl4emn 129 14 the the DT cord-255350-dmbl4emn 129 15 length length NN cord-255350-dmbl4emn 129 16 of of IN cord-255350-dmbl4emn 129 17 the the DT cord-255350-dmbl4emn 129 18 C c NN cord-255350-dmbl4emn 129 19 0 0 CD cord-255350-dmbl4emn 129 20 C c NN cord-255350-dmbl4emn 129 21 00 00 NFP cord-255350-dmbl4emn 129 22 or or CC cord-255350-dmbl4emn 129 23 the the DT cord-255350-dmbl4emn 129 24 C c NN cord-255350-dmbl4emn 129 25 00 00 HYPH cord-255350-dmbl4emn 129 26 D d NN cord-255350-dmbl4emn 129 27 loops loop NNS cord-255350-dmbl4emn 129 28 , , , cord-255350-dmbl4emn 129 29 the the DT cord-255350-dmbl4emn 129 30 C C NNP cord-255350-dmbl4emn 129 31 00 00 CD cord-255350-dmbl4emn 129 32 strand strand NN cord-255350-dmbl4emn 129 33 is be VBZ cord-255350-dmbl4emn 129 34 found find VBN cord-255350-dmbl4emn 129 35 to to TO cord-255350-dmbl4emn 129 36 be be VB cord-255350-dmbl4emn 129 37 distorted distort VBN cord-255350-dmbl4emn 129 38 relative relative JJ cord-255350-dmbl4emn 129 39 to to IN cord-255350-dmbl4emn 129 40 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 130 1 ( ( -LRB- cord-255350-dmbl4emn 130 2 Fig Fig NNP cord-255350-dmbl4emn 130 3 . . . cord-255350-dmbl4emn 130 4 3a 3a LS cord-255350-dmbl4emn 130 5 ) ) -RRB- cord-255350-dmbl4emn 131 1 and and CC cord-255350-dmbl4emn 131 2 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 132 1 ( ( -LRB- cord-255350-dmbl4emn 132 2 Fig Fig NNP cord-255350-dmbl4emn 132 3 . . . cord-255350-dmbl4emn 132 4 3b 3b CD cord-255350-dmbl4emn 132 5 ) ) -RRB- cord-255350-dmbl4emn 132 6 . . . cord-255350-dmbl4emn 133 1 The the DT cord-255350-dmbl4emn 133 2 C c NN cord-255350-dmbl4emn 133 3 00 00 CD cord-255350-dmbl4emn 133 4 strand strand NN cord-255350-dmbl4emn 133 5 bulges bulge NNS cord-255350-dmbl4emn 133 6 in in IN cord-255350-dmbl4emn 133 7 the the DT cord-255350-dmbl4emn 133 8 center center NN cord-255350-dmbl4emn 133 9 ( ( -LRB- cord-255350-dmbl4emn 133 10 residues residue NNS cord-255350-dmbl4emn 133 11 56 56 CD cord-255350-dmbl4emn 133 12 - - SYM cord-255350-dmbl4emn 133 13 60 60 CD cord-255350-dmbl4emn 133 14 ) ) -RRB- cord-255350-dmbl4emn 133 15 , , , cord-255350-dmbl4emn 133 16 causing cause VBG cord-255350-dmbl4emn 133 17 breakages breakage NNS cord-255350-dmbl4emn 133 18 of of IN cord-255350-dmbl4emn 133 19 hydrogen hydrogen NN cord-255350-dmbl4emn 133 20 bonds bond NNS cord-255350-dmbl4emn 133 21 in in IN cord-255350-dmbl4emn 133 22 the the DT cord-255350-dmbl4emn 133 23 antiparallel antiparallel NN cord-255350-dmbl4emn 133 24 -sheet -sheet : cord-255350-dmbl4emn 133 25 between between IN cord-255350-dmbl4emn 133 26 the the DT cord-255350-dmbl4emn 133 27 C C NNP cord-255350-dmbl4emn 133 28 0 0 CD cord-255350-dmbl4emn 133 29 and and CC cord-255350-dmbl4emn 133 30 C c NN cord-255350-dmbl4emn 133 31 00 00 CD cord-255350-dmbl4emn 133 32 strands strand NNS cord-255350-dmbl4emn 133 33 . . . cord-255350-dmbl4emn 134 1 The the DT cord-255350-dmbl4emn 134 2 C C NNP cord-255350-dmbl4emn 134 3 00 00 CD cord-255350-dmbl4emn 134 4 strand strand NN cord-255350-dmbl4emn 134 5 of of IN cord-255350-dmbl4emn 134 6 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 134 7 is be VBZ cord-255350-dmbl4emn 134 8 held hold VBN cord-255350-dmbl4emn 134 9 in in IN cord-255350-dmbl4emn 134 10 place place NN cord-255350-dmbl4emn 134 11 through through IN cord-255350-dmbl4emn 134 12 a a DT cord-255350-dmbl4emn 134 13 hydrogen hydrogen NN cord-255350-dmbl4emn 134 14 bond bond NN cord-255350-dmbl4emn 134 15 from from IN cord-255350-dmbl4emn 134 16 the the DT cord-255350-dmbl4emn 134 17 O O NNP cord-255350-dmbl4emn 134 18 " " '' cord-255350-dmbl4emn 134 19 2 2 CD cord-255350-dmbl4emn 134 20 atom atom NN cord-255350-dmbl4emn 134 21 of of IN cord-255350-dmbl4emn 134 22 Asn57 Asn57 NNP cord-255350-dmbl4emn 134 23 to to IN cord-255350-dmbl4emn 134 24 the the DT cord-255350-dmbl4emn 134 25 N n CD cord-255350-dmbl4emn 134 26 atom atom NN cord-255350-dmbl4emn 134 27 of of IN cord-255350-dmbl4emn 134 28 Gly48 Gly48 NNP cord-255350-dmbl4emn 134 29 ( ( -LRB- cord-255350-dmbl4emn 134 30 Fig Fig NNP cord-255350-dmbl4emn 134 31 . . . cord-255350-dmbl4emn 134 32 3c 3c NNP cord-255350-dmbl4emn 134 33 ) ) -RRB- cord-255350-dmbl4emn 134 34 . . . cord-255350-dmbl4emn 135 1 In in IN cord-255350-dmbl4emn 135 2 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 135 3 the the DT cord-255350-dmbl4emn 135 4 carbonyl carbonyl NN cord-255350-dmbl4emn 135 5 group group NN cord-255350-dmbl4emn 135 6 of of IN cord-255350-dmbl4emn 135 7 Thr57 Thr57 NNP cord-255350-dmbl4emn 135 8 forms form VBZ cord-255350-dmbl4emn 135 9 the the DT cord-255350-dmbl4emn 135 10 hydrogen hydrogen NN cord-255350-dmbl4emn 135 11 bond bond NN cord-255350-dmbl4emn 135 12 to to IN cord-255350-dmbl4emn 135 13 Gly48 Gly48 NNP cord-255350-dmbl4emn 135 14 ( ( -LRB- cord-255350-dmbl4emn 135 15 Fig Fig NNP cord-255350-dmbl4emn 135 16 . . . cord-255350-dmbl4emn 135 17 3d 3d NNP cord-255350-dmbl4emn 135 18 ) ) -RRB- cord-255350-dmbl4emn 135 19 . . . cord-255350-dmbl4emn 136 1 Although although IN cord-255350-dmbl4emn 136 2 the the DT cord-255350-dmbl4emn 136 3 C C NNP cord-255350-dmbl4emn 136 4 00 00 CD cord-255350-dmbl4emn 136 5 strand strand NN cord-255350-dmbl4emn 136 6 does do VBZ cord-255350-dmbl4emn 136 7 not not RB cord-255350-dmbl4emn 136 8 buckle buckle VB cord-255350-dmbl4emn 136 9 in in IN cord-255350-dmbl4emn 136 10 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 136 11 , , , cord-255350-dmbl4emn 136 12 as as IN cord-255350-dmbl4emn 136 13 in in IN cord-255350-dmbl4emn 136 14 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 136 15 , , , cord-255350-dmbl4emn 136 16 it -PRON- PRP cord-255350-dmbl4emn 136 17 is be VBZ cord-255350-dmbl4emn 136 18 found find VBN cord-255350-dmbl4emn 136 19 that that IN cord-255350-dmbl4emn 136 20 Thr57 Thr57 NNP cord-255350-dmbl4emn 136 21 ( ( -LRB- cord-255350-dmbl4emn 136 22 a a DT cord-255350-dmbl4emn 136 23 ) ) -RRB- cord-255350-dmbl4emn 136 24 Superposition superposition NN cord-255350-dmbl4emn 136 25 of of IN cord-255350-dmbl4emn 136 26 the the DT cord-255350-dmbl4emn 136 27 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 136 28 dimer dimer NNP cord-255350-dmbl4emn 136 29 ( ( -LRB- cord-255350-dmbl4emn 136 30 CEA7A CEA7A NNP cord-255350-dmbl4emn 136 31 and and CC cord-255350-dmbl4emn 136 32 CEA7B CEA7B NNP cord-255350-dmbl4emn 136 33 ; ; : cord-255350-dmbl4emn 136 34 red red JJ cord-255350-dmbl4emn 136 35 and and CC cord-255350-dmbl4emn 136 36 pink pink JJ cord-255350-dmbl4emn 136 37 , , , cord-255350-dmbl4emn 136 38 respectively respectively RB cord-255350-dmbl4emn 136 39 ) ) -RRB- cord-255350-dmbl4emn 136 40 onto onto IN cord-255350-dmbl4emn 136 41 the the DT cord-255350-dmbl4emn 136 42 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 136 43 dimer dimer NN cord-255350-dmbl4emn 136 44 ( ( -LRB- cord-255350-dmbl4emn 136 45 CEA5A CEA5A NNP cord-255350-dmbl4emn 136 46 and and CC cord-255350-dmbl4emn 136 47 CEA5B CEA5B JJS cord-255350-dmbl4emn 136 48 ; ; : cord-255350-dmbl4emn 136 49 light light NN cord-255350-dmbl4emn 136 50 and and CC cord-255350-dmbl4emn 136 51 dark dark JJ cord-255350-dmbl4emn 136 52 cyan cyan NN cord-255350-dmbl4emn 136 53 , , , cord-255350-dmbl4emn 136 54 respectively respectively RB cord-255350-dmbl4emn 136 55 ) ) -RRB- cord-255350-dmbl4emn 136 56 through through IN cord-255350-dmbl4emn 136 57 molecule molecule NN cord-255350-dmbl4emn 136 58 A. a. NN cord-255350-dmbl4emn 137 1 ( ( -LRB- cord-255350-dmbl4emn 137 2 b b LS cord-255350-dmbl4emn 137 3 ) ) -RRB- cord-255350-dmbl4emn 137 4 Superposition superposition NN cord-255350-dmbl4emn 137 5 of of IN cord-255350-dmbl4emn 137 6 the the DT cord-255350-dmbl4emn 137 7 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 137 8 dimer dimer NNP cord-255350-dmbl4emn 137 9 ( ( -LRB- cord-255350-dmbl4emn 137 10 CEA7A CEA7A NNP cord-255350-dmbl4emn 137 11 and and CC cord-255350-dmbl4emn 137 12 CEA7B CEA7B NNP cord-255350-dmbl4emn 137 13 ; ; : cord-255350-dmbl4emn 137 14 red red JJ cord-255350-dmbl4emn 137 15 and and CC cord-255350-dmbl4emn 137 16 pink pink JJ cord-255350-dmbl4emn 137 17 , , , cord-255350-dmbl4emn 137 18 respectively respectively RB cord-255350-dmbl4emn 137 19 ) ) -RRB- cord-255350-dmbl4emn 137 20 onto onto IN cord-255350-dmbl4emn 137 21 the the DT cord-255350-dmbl4emn 137 22 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 137 23 dimer dimer NN cord-255350-dmbl4emn 137 24 ( ( -LRB- cord-255350-dmbl4emn 137 25 CEA1A cea1a CD cord-255350-dmbl4emn 137 26 and and CC cord-255350-dmbl4emn 137 27 CEA1B cea1b NN cord-255350-dmbl4emn 137 28 ; ; . cord-255350-dmbl4emn 138 1 blue blue JJ cord-255350-dmbl4emn 138 2 and and CC cord-255350-dmbl4emn 138 3 lilac lilac NN cord-255350-dmbl4emn 138 4 , , , cord-255350-dmbl4emn 138 5 respectively respectively RB cord-255350-dmbl4emn 138 6 ) ) -RRB- cord-255350-dmbl4emn 138 7 through through IN cord-255350-dmbl4emn 138 8 molecule molecule NN cord-255350-dmbl4emn 138 9 A. a. NN cord-255350-dmbl4emn 138 10 ( ( -LRB- cord-255350-dmbl4emn 138 11 c c NN cord-255350-dmbl4emn 138 12 ) ) -RRB- cord-255350-dmbl4emn 138 13 Alignment Alignment NNP cord-255350-dmbl4emn 138 14 of of IN cord-255350-dmbl4emn 138 15 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 138 16 and and CC cord-255350-dmbl4emn 138 17 CEACAM5 CEACAM5 NNP cord-255350-dmbl4emn 138 18 monomers monomer NNS cord-255350-dmbl4emn 138 19 , , , cord-255350-dmbl4emn 138 20 colored color VBN cord-255350-dmbl4emn 138 21 by by IN cord-255350-dmbl4emn 138 22 r.m.s.d r.m.s.d NNP cord-255350-dmbl4emn 138 23 . . . cord-255350-dmbl4emn 139 1 Dark dark JJ cord-255350-dmbl4emn 139 2 blue blue NN cord-255350-dmbl4emn 139 3 is be VBZ cord-255350-dmbl4emn 139 4 low low JJ cord-255350-dmbl4emn 139 5 r.m.s.d r.m.s.d NNP cord-255350-dmbl4emn 139 6 . . . cord-255350-dmbl4emn 140 1 and and CC cord-255350-dmbl4emn 140 2 red red NN cord-255350-dmbl4emn 140 3 is be VBZ cord-255350-dmbl4emn 140 4 high high JJ cord-255350-dmbl4emn 140 5 r.m.s.d r.m.s.d NNP cord-255350-dmbl4emn 140 6 . . . cord-255350-dmbl4emn 141 1 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 141 2 is be VBZ cord-255350-dmbl4emn 141 3 omitted omit VBN cord-255350-dmbl4emn 141 4 for for IN cord-255350-dmbl4emn 141 5 clarity clarity NN cord-255350-dmbl4emn 141 6 . . . cord-255350-dmbl4emn 142 1 ( ( -LRB- cord-255350-dmbl4emn 142 2 d d NN cord-255350-dmbl4emn 142 3 ) ) -RRB- cord-255350-dmbl4emn 143 1 Potential potential JJ cord-255350-dmbl4emn 143 2 N n CD cord-255350-dmbl4emn 143 3 - - HYPH cord-255350-dmbl4emn 143 4 linked link VBN cord-255350-dmbl4emn 143 5 glycosylation glycosylation NN cord-255350-dmbl4emn 143 6 sites site NNS cord-255350-dmbl4emn 143 7 of of IN cord-255350-dmbl4emn 143 8 the the DT cord-255350-dmbl4emn 143 9 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 143 10 dimer dimer NNP cord-255350-dmbl4emn 143 11 . . . cord-255350-dmbl4emn 144 1 All all DT cord-255350-dmbl4emn 144 2 residues residue NNS cord-255350-dmbl4emn 144 3 are be VBP cord-255350-dmbl4emn 144 4 solvent solvent NN cord-255350-dmbl4emn 144 5 - - HYPH cord-255350-dmbl4emn 144 6 exposed expose VBN cord-255350-dmbl4emn 144 7 and and CC cord-255350-dmbl4emn 144 8 are be VBP cord-255350-dmbl4emn 144 9 not not RB cord-255350-dmbl4emn 144 10 found find VBN cord-255350-dmbl4emn 144 11 in in IN cord-255350-dmbl4emn 144 12 the the DT cord-255350-dmbl4emn 144 13 dimerization dimerization NN cord-255350-dmbl4emn 144 14 interface interface NN cord-255350-dmbl4emn 144 15 . . . cord-255350-dmbl4emn 145 1 does do VBZ cord-255350-dmbl4emn 145 2 form form VB cord-255350-dmbl4emn 145 3 a a DT cord-255350-dmbl4emn 145 4 hydrogen hydrogen NN cord-255350-dmbl4emn 145 5 bond bond NN cord-255350-dmbl4emn 145 6 across across IN cord-255350-dmbl4emn 145 7 the the DT cord-255350-dmbl4emn 145 8 dimerization dimerization NN cord-255350-dmbl4emn 145 9 interface interface NN cord-255350-dmbl4emn 145 10 to to IN cord-255350-dmbl4emn 145 11 Asp95 Asp95 NNP cord-255350-dmbl4emn 145 12 ( ( -LRB- cord-255350-dmbl4emn 145 13 Fig Fig NNP cord-255350-dmbl4emn 145 14 . . . cord-255350-dmbl4emn 145 15 3e 3e NNP cord-255350-dmbl4emn 145 16 ) ) -RRB- cord-255350-dmbl4emn 145 17 . . . cord-255350-dmbl4emn 146 1 This this DT cord-255350-dmbl4emn 146 2 buckling buckling NN cord-255350-dmbl4emn 146 3 of of IN cord-255350-dmbl4emn 146 4 the the DT cord-255350-dmbl4emn 146 5 C c NN cord-255350-dmbl4emn 146 6 00 00 CD cord-255350-dmbl4emn 146 7 strand strand NN cord-255350-dmbl4emn 146 8 creates create VBZ cord-255350-dmbl4emn 146 9 an an DT cord-255350-dmbl4emn 146 10 extra extra JJ cord-255350-dmbl4emn 146 11 interaction interaction NN cord-255350-dmbl4emn 146 12 in in IN cord-255350-dmbl4emn 146 13 the the DT cord-255350-dmbl4emn 146 14 dimerization dimerization NN cord-255350-dmbl4emn 146 15 interface interface NN cord-255350-dmbl4emn 146 16 , , , cord-255350-dmbl4emn 146 17 potentially potentially RB cord-255350-dmbl4emn 146 18 explaining explain VBG cord-255350-dmbl4emn 146 19 why why WRB cord-255350-dmbl4emn 146 20 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 146 21 forms form NNS cord-255350-dmbl4emn 146 22 such such JJ cord-255350-dmbl4emn 146 23 tight tight JJ cord-255350-dmbl4emn 146 24 dimers dimer NNS cord-255350-dmbl4emn 146 25 and and CC cord-255350-dmbl4emn 146 26 has have VBZ cord-255350-dmbl4emn 146 27 not not RB cord-255350-dmbl4emn 146 28 been be VBN cord-255350-dmbl4emn 146 29 found find VBN cord-255350-dmbl4emn 146 30 to to TO cord-255350-dmbl4emn 146 31 form form VB cord-255350-dmbl4emn 146 32 heterodimeric heterodimeric JJ cord-255350-dmbl4emn 146 33 CEACAM CEACAM NNP cord-255350-dmbl4emn 146 34 complexes complex NNS cord-255350-dmbl4emn 146 35 such such JJ cord-255350-dmbl4emn 146 36 as as IN cord-255350-dmbl4emn 146 37 CEACAM6-CEACAM8 CEACAM6-CEACAM8 NNP cord-255350-dmbl4emn 146 38 , , , cord-255350-dmbl4emn 146 39 CEACAM1-CEACAM8 CEACAM1-CEACAM8 NNP cord-255350-dmbl4emn 146 40 , , , cord-255350-dmbl4emn 146 41 CEACAM1-CEACAM6 CEACAM1-CEACAM6 NNP cord-255350-dmbl4emn 146 42 , , , cord-255350-dmbl4emn 146 43 CEACAM3-CEACAM6 CEACAM3-CEACAM6 NNP cord-255350-dmbl4emn 146 44 and and CC cord-255350-dmbl4emn 146 45 CEACAM5-CEACAM6 CEACAM5-CEACAM6 NNP cord-255350-dmbl4emn 146 46 ( ( -LRB- cord-255350-dmbl4emn 146 47 Oikawa Oikawa NNP cord-255350-dmbl4emn 146 48 et et NNP cord-255350-dmbl4emn 146 49 al al NNP cord-255350-dmbl4emn 146 50 . . NNP cord-255350-dmbl4emn 146 51 , , , cord-255350-dmbl4emn 146 52 1991 1991 CD cord-255350-dmbl4emn 146 53 ; ; : cord-255350-dmbl4emn 146 54 Skubitz Skubitz NNP cord-255350-dmbl4emn 146 55 & & CC cord-255350-dmbl4emn 146 56 Skubitz Skubitz NNP cord-255350-dmbl4emn 146 57 , , , cord-255350-dmbl4emn 146 58 2008 2008 CD cord-255350-dmbl4emn 146 59 ; ; : cord-255350-dmbl4emn 146 60 Singer Singer NNP cord-255350-dmbl4emn 146 61 et et NNP cord-255350-dmbl4emn 146 62 al al NNP cord-255350-dmbl4emn 146 63 . . . cord-255350-dmbl4emn 147 1 , , , cord-255350-dmbl4emn 147 2 2014 2014 CD cord-255350-dmbl4emn 147 3 ) ) -RRB- cord-255350-dmbl4emn 147 4 . . . cord-255350-dmbl4emn 148 1 Although although IN cord-255350-dmbl4emn 148 2 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 148 3 does do VBZ cord-255350-dmbl4emn 148 4 not not RB cord-255350-dmbl4emn 148 5 buckle buckle VB cord-255350-dmbl4emn 148 6 , , , cord-255350-dmbl4emn 148 7 it -PRON- PRP cord-255350-dmbl4emn 148 8 too too RB cord-255350-dmbl4emn 148 9 creates create VBZ cord-255350-dmbl4emn 148 10 an an DT cord-255350-dmbl4emn 148 11 extra extra JJ cord-255350-dmbl4emn 148 12 interaction interaction NN cord-255350-dmbl4emn 148 13 across across IN cord-255350-dmbl4emn 148 14 the the DT cord-255350-dmbl4emn 148 15 dimerization dimerization NN cord-255350-dmbl4emn 148 16 through through IN cord-255350-dmbl4emn 148 17 reorientation reorientation NN cord-255350-dmbl4emn 148 18 of of IN cord-255350-dmbl4emn 148 19 Asp95 Asp95 NNP cord-255350-dmbl4emn 148 20 . . . cord-255350-dmbl4emn 149 1 This this DT cord-255350-dmbl4emn 149 2 also also RB cord-255350-dmbl4emn 149 3 suggests suggest VBZ cord-255350-dmbl4emn 149 4 that that IN cord-255350-dmbl4emn 149 5 this this DT cord-255350-dmbl4emn 149 6 hydrogen hydrogen NN cord-255350-dmbl4emn 149 7 bond bond NN cord-255350-dmbl4emn 149 8 is be VBZ cord-255350-dmbl4emn 149 9 important important JJ cord-255350-dmbl4emn 149 10 for for IN cord-255350-dmbl4emn 149 11 a a DT cord-255350-dmbl4emn 149 12 higher high JJR cord-255350-dmbl4emn 149 13 affinity affinity NN cord-255350-dmbl4emn 149 14 interaction interaction NN cord-255350-dmbl4emn 149 15 . . . cord-255350-dmbl4emn 150 1 The the DT cord-255350-dmbl4emn 150 2 structure structure NN cord-255350-dmbl4emn 150 3 of of IN cord-255350-dmbl4emn 150 4 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 150 5 reveals reveal VBZ cord-255350-dmbl4emn 150 6 that that IN cord-255350-dmbl4emn 150 7 the the DT cord-255350-dmbl4emn 150 8 dimerization dimerization NN cord-255350-dmbl4emn 150 9 interface interface NN cord-255350-dmbl4emn 150 10 is be VBZ cord-255350-dmbl4emn 150 11 comprised comprise VBN cord-255350-dmbl4emn 150 12 of of IN cord-255350-dmbl4emn 150 13 the the DT cord-255350-dmbl4emn 150 14 same same JJ cord-255350-dmbl4emn 150 15 face face NN cord-255350-dmbl4emn 150 16 as as IN cord-255350-dmbl4emn 150 17 other other JJ cord-255350-dmbl4emn 150 18 CEACAMs ceacam NNS cord-255350-dmbl4emn 150 19 ( ( -LRB- cord-255350-dmbl4emn 150 20 GFCC GFCC NNP cord-255350-dmbl4emn 150 21 0 0 CD cord-255350-dmbl4emn 150 22 C c NN cord-255350-dmbl4emn 150 23 00 00 NFP cord-255350-dmbl4emn 150 24 ) ) -RRB- cord-255350-dmbl4emn 150 25 yet yet CC cord-255350-dmbl4emn 150 26 can can MD cord-255350-dmbl4emn 150 27 accommodate accommodate VB cord-255350-dmbl4emn 150 28 16 16 CD cord-255350-dmbl4emn 150 29 different different JJ cord-255350-dmbl4emn 150 30 residues residue NNS cord-255350-dmbl4emn 150 31 ( ( -LRB- cord-255350-dmbl4emn 150 32 eight eight CD cord-255350-dmbl4emn 150 33 from from IN cord-255350-dmbl4emn 150 34 each each DT cord-255350-dmbl4emn 150 35 monomer monomer NN cord-255350-dmbl4emn 150 36 ) ) -RRB- cord-255350-dmbl4emn 150 37 , , , cord-255350-dmbl4emn 150 38 suggesting suggest VBG cord-255350-dmbl4emn 150 39 that that IN cord-255350-dmbl4emn 150 40 these these DT cord-255350-dmbl4emn 150 41 sequence sequence NN cord-255350-dmbl4emn 150 42 differences difference NNS cord-255350-dmbl4emn 150 43 can can MD cord-255350-dmbl4emn 150 44 modulate modulate VB cord-255350-dmbl4emn 150 45 the the DT cord-255350-dmbl4emn 150 46 homodimerization homodimerization NN cord-255350-dmbl4emn 150 47 to to TO cord-255350-dmbl4emn 150 48 achieve achieve VB cord-255350-dmbl4emn 150 49 a a DT cord-255350-dmbl4emn 150 50 tenfold tenfold JJ cord-255350-dmbl4emn 150 51 increase increase NN cord-255350-dmbl4emn 150 52 in in IN cord-255350-dmbl4emn 150 53 affinity affinity NN cord-255350-dmbl4emn 150 54 . . . cord-255350-dmbl4emn 151 1 ( ( -LRB- cord-255350-dmbl4emn 151 2 a a LS cord-255350-dmbl4emn 151 3 ) ) -RRB- cord-255350-dmbl4emn 152 1 The the DT cord-255350-dmbl4emn 152 2 polypeptide polypeptide NN cord-255350-dmbl4emn 152 3 backbone backbone NN cord-255350-dmbl4emn 152 4 of of IN cord-255350-dmbl4emn 152 5 the the DT cord-255350-dmbl4emn 152 6 C C NNP cord-255350-dmbl4emn 152 7 0 0 CD cord-255350-dmbl4emn 152 8 and and CC cord-255350-dmbl4emn 152 9 C c NN cord-255350-dmbl4emn 152 10 00 00 CD cord-255350-dmbl4emn 152 11 strands strand NNS cord-255350-dmbl4emn 152 12 of of IN cord-255350-dmbl4emn 152 13 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 152 14 ( ( -LRB- cord-255350-dmbl4emn 152 15 red red NNP cord-255350-dmbl4emn 152 16 ) ) -RRB- cord-255350-dmbl4emn 152 17 and and CC cord-255350-dmbl4emn 153 1 CEACAM5 CEACAM5 NNP cord-255350-dmbl4emn 154 1 ( ( -LRB- cord-255350-dmbl4emn 154 2 cyan cyan NNP cord-255350-dmbl4emn 154 3 ) ) -RRB- cord-255350-dmbl4emn 154 4 . . . cord-255350-dmbl4emn 155 1 ( ( -LRB- cord-255350-dmbl4emn 155 2 b b LS cord-255350-dmbl4emn 155 3 ) ) -RRB- cord-255350-dmbl4emn 155 4 The the DT cord-255350-dmbl4emn 155 5 polypeptide polypeptide NN cord-255350-dmbl4emn 155 6 backbone backbone NN cord-255350-dmbl4emn 155 7 of of IN cord-255350-dmbl4emn 155 8 the the DT cord-255350-dmbl4emn 155 9 C C NNP cord-255350-dmbl4emn 155 10 0 0 CD cord-255350-dmbl4emn 155 11 and and CC cord-255350-dmbl4emn 155 12 C c NN cord-255350-dmbl4emn 155 13 00 00 CD cord-255350-dmbl4emn 155 14 strands strand NNS cord-255350-dmbl4emn 155 15 of of IN cord-255350-dmbl4emn 155 16 CEACAM7 ceacam7 XX cord-255350-dmbl4emn 156 1 ( ( -LRB- cord-255350-dmbl4emn 156 2 red red NNP cord-255350-dmbl4emn 156 3 ) ) -RRB- cord-255350-dmbl4emn 156 4 and and CC cord-255350-dmbl4emn 156 5 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 157 1 ( ( -LRB- cord-255350-dmbl4emn 157 2 blue blue NNP cord-255350-dmbl4emn 157 3 ) ) -RRB- cord-255350-dmbl4emn 157 4 . . . cord-255350-dmbl4emn 158 1 ( ( -LRB- cord-255350-dmbl4emn 158 2 c c NN cord-255350-dmbl4emn 158 3 ) ) -RRB- cord-255350-dmbl4emn 159 1 The the DT cord-255350-dmbl4emn 159 2 insertion insertion NN cord-255350-dmbl4emn 159 3 of of IN cord-255350-dmbl4emn 159 4 Ser54 Ser54 NNP cord-255350-dmbl4emn 159 5 in in IN cord-255350-dmbl4emn 159 6 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 159 7 ( ( -LRB- cord-255350-dmbl4emn 159 8 red red NNP cord-255350-dmbl4emn 159 9 ) ) -RRB- cord-255350-dmbl4emn 159 10 results result NNS cord-255350-dmbl4emn 159 11 in in IN cord-255350-dmbl4emn 159 12 breakage breakage NN cord-255350-dmbl4emn 159 13 of of IN cord-255350-dmbl4emn 159 14 the the DT cord-255350-dmbl4emn 159 15 main main JJ cord-255350-dmbl4emn 159 16 - - HYPH cord-255350-dmbl4emn 159 17 chain chain NN cord-255350-dmbl4emn 159 18 antiparallel antiparallel IN cord-255350-dmbl4emn 159 19 hydrogen hydrogen NN cord-255350-dmbl4emn 159 20 bonds bond NNS cord-255350-dmbl4emn 159 21 and and CC cord-255350-dmbl4emn 159 22 replacement replacement NN cord-255350-dmbl4emn 159 23 with with IN cord-255350-dmbl4emn 159 24 a a DT cord-255350-dmbl4emn 159 25 hydrogen hydrogen NN cord-255350-dmbl4emn 159 26 bond bond NN cord-255350-dmbl4emn 159 27 from from IN cord-255350-dmbl4emn 159 28 the the DT cord-255350-dmbl4emn 159 29 side side NN cord-255350-dmbl4emn 159 30 chain chain NN cord-255350-dmbl4emn 159 31 of of IN cord-255350-dmbl4emn 159 32 Asn57 Asn57 NNP cord-255350-dmbl4emn 159 33 . . . cord-255350-dmbl4emn 160 1 This this DT cord-255350-dmbl4emn 160 2 residue residue NN cord-255350-dmbl4emn 160 3 also also RB cord-255350-dmbl4emn 160 4 forms form VBZ cord-255350-dmbl4emn 160 5 a a DT cord-255350-dmbl4emn 160 6 hydrogen hydrogen NN cord-255350-dmbl4emn 160 7 bond bond NN cord-255350-dmbl4emn 160 8 across across IN cord-255350-dmbl4emn 160 9 the the DT cord-255350-dmbl4emn 160 10 dimer dimer NN cord-255350-dmbl4emn 160 11 interface interface NN cord-255350-dmbl4emn 160 12 to to IN cord-255350-dmbl4emn 160 13 Asn96 Asn96 NNP cord-255350-dmbl4emn 160 14 ( ( -LRB- cord-255350-dmbl4emn 160 15 brown brown NNP cord-255350-dmbl4emn 160 16 ) ) -RRB- cord-255350-dmbl4emn 160 17 . . . cord-255350-dmbl4emn 161 1 ( ( -LRB- cord-255350-dmbl4emn 161 2 d d NN cord-255350-dmbl4emn 161 3 ) ) -RRB- cord-255350-dmbl4emn 162 1 In in IN cord-255350-dmbl4emn 162 2 CEACAM5 ceacam5 NN cord-255350-dmbl4emn 162 3 ( ( -LRB- cord-255350-dmbl4emn 162 4 green green JJ cord-255350-dmbl4emn 162 5 ) ) -RRB- cord-255350-dmbl4emn 162 6 , , , cord-255350-dmbl4emn 162 7 only only RB cord-255350-dmbl4emn 162 8 the the DT cord-255350-dmbl4emn 162 9 main main JJ cord-255350-dmbl4emn 162 10 - - HYPH cord-255350-dmbl4emn 162 11 chain chain NN cord-255350-dmbl4emn 163 1 antiparallel antiparallel RB cord-255350-dmbl4emn 164 1 hydrogen hydrogen NN cord-255350-dmbl4emn 164 2 bonds bond NNS cord-255350-dmbl4emn 164 3 exist exist VBP cord-255350-dmbl4emn 164 4 . . . cord-255350-dmbl4emn 165 1 Thr57 Thr57 NNP cord-255350-dmbl4emn 165 2 is be VBZ cord-255350-dmbl4emn 165 3 too too RB cord-255350-dmbl4emn 165 4 short short JJ cord-255350-dmbl4emn 165 5 to to TO cord-255350-dmbl4emn 165 6 form form VB cord-255350-dmbl4emn 165 7 a a DT cord-255350-dmbl4emn 165 8 hydrogen hydrogen NN cord-255350-dmbl4emn 165 9 bond bond NN cord-255350-dmbl4emn 165 10 across across IN cord-255350-dmbl4emn 165 11 the the DT cord-255350-dmbl4emn 165 12 dimer dimer NN cord-255350-dmbl4emn 165 13 interface interface NN cord-255350-dmbl4emn 165 14 to to IN cord-255350-dmbl4emn 165 15 Asp95 Asp95 NNP cord-255350-dmbl4emn 165 16 ( ( -LRB- cord-255350-dmbl4emn 165 17 purple purple NN cord-255350-dmbl4emn 165 18 ) ) -RRB- cord-255350-dmbl4emn 165 19 . . . cord-255350-dmbl4emn 166 1 ( ( -LRB- cord-255350-dmbl4emn 166 2 e e LS cord-255350-dmbl4emn 166 3 ) ) -RRB- cord-255350-dmbl4emn 167 1 However however RB cord-255350-dmbl4emn 167 2 , , , cord-255350-dmbl4emn 167 3 this this DT cord-255350-dmbl4emn 167 4 is be VBZ cord-255350-dmbl4emn 167 5 not not RB cord-255350-dmbl4emn 167 6 the the DT cord-255350-dmbl4emn 167 7 case case NN cord-255350-dmbl4emn 167 8 for for IN cord-255350-dmbl4emn 167 9 CEACAM1 CEACAM1 NNP cord-255350-dmbl4emn 167 10 ( ( -LRB- cord-255350-dmbl4emn 167 11 blue blue NNP cord-255350-dmbl4emn 167 12 ) ) -RRB- cord-255350-dmbl4emn 167 13 . . . cord-255350-dmbl4emn 168 1 Thr57 Thr57 NNP cord-255350-dmbl4emn 168 2 forms form VBZ cord-255350-dmbl4emn 168 3 a a DT cord-255350-dmbl4emn 168 4 hydrogen hydrogen NN cord-255350-dmbl4emn 168 5 bond bond NN cord-255350-dmbl4emn 168 6 across across IN cord-255350-dmbl4emn 168 7 the the DT cord-255350-dmbl4emn 168 8 dimer dimer NN cord-255350-dmbl4emn 168 9 interface interface NN cord-255350-dmbl4emn 168 10 to to IN cord-255350-dmbl4emn 168 11 Asp95 Asp95 NNP cord-255350-dmbl4emn 168 12 ( ( -LRB- cord-255350-dmbl4emn 168 13 gray gray JJ cord-255350-dmbl4emn 168 14 ) ) -RRB- cord-255350-dmbl4emn 168 15 . . . cord-255350-dmbl4emn 169 1 N n CD cord-255350-dmbl4emn 169 2 - - HYPH cord-255350-dmbl4emn 169 3 terminal terminal JJ cord-255350-dmbl4emn 169 4 dimerization dimerization NN cord-255350-dmbl4emn 169 5 domain domain NN cord-255350-dmbl4emn 169 6 of of IN cord-255350-dmbl4emn 169 7 CEACAM7 CEACAM7 NNP cord-255350-dmbl4emn 169 8 Lung Lung NNP cord-255350-dmbl4emn 169 9 Cancer Cancer NNP cord-255350-dmbl4emn 169 10 Proc Proc NNP cord-255350-dmbl4emn 169 11 . . . cord-255350-dmbl4emn 170 1 Natl Natl NNP cord-255350-dmbl4emn 170 2 Acad Acad NNP cord-255350-dmbl4emn 170 3 . . . cord-255350-dmbl4emn 171 1 Sci Sci NNP cord-255350-dmbl4emn 171 2 . . NNP cord-255350-dmbl4emn 171 3 USA USA NNP cord-255350-dmbl4emn 172 1 We -PRON- PRP cord-255350-dmbl4emn 172 2 thank thank VBP cord-255350-dmbl4emn 172 3 the the DT cord-255350-dmbl4emn 172 4 staff staff NN cord-255350-dmbl4emn 172 5 of of IN cord-255350-dmbl4emn 172 6 the the DT cord-255350-dmbl4emn 172 7 Advanced Advanced NNP cord-255350-dmbl4emn 172 8 Photon Photon NNP cord-255350-dmbl4emn 172 9 Source Source NNP cord-255350-dmbl4emn 172 10 ( ( -LRB- cord-255350-dmbl4emn 172 11 APS APS NNP cord-255350-dmbl4emn 172 12 ) ) -RRB- cord-255350-dmbl4emn 172 13 GM GM NNP cord-255350-dmbl4emn 172 14 / / SYM cord-255350-dmbl4emn 172 15 CA CA NNP cord-255350-dmbl4emn 172 16 CAT CAT NNP cord-255350-dmbl4emn 172 17 beamline beamline NN cord-255350-dmbl4emn 172 18 23-ID 23-id CD cord-255350-dmbl4emn 172 19 - - HYPH cord-255350-dmbl4emn 172 20 B b NN cord-255350-dmbl4emn 172 21 for for IN cord-255350-dmbl4emn 172 22 their -PRON- PRP$ cord-255350-dmbl4emn 172 23 support support NN cord-255350-dmbl4emn 172 24 . . . cord-255350-dmbl4emn 173 1 The the DT cord-255350-dmbl4emn 173 2 authors author NNS cord-255350-dmbl4emn 173 3 declare declare VBP cord-255350-dmbl4emn 173 4 no no DT cord-255350-dmbl4emn 173 5 competing compete VBG cord-255350-dmbl4emn 173 6 financial financial JJ cord-255350-dmbl4emn 173 7 interests interest NNS cord-255350-dmbl4emn 173 8 . . .