id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002838-thygu6at	Lanfranco, Maria Fe	Glial- and Neuronal-Specific Expression of CCL5 mRNA in the Rat Brain	2018-01-12		.txt	text/plain	5806	310	50	In this work, we have used fluorescence in situ hybridization combined with immunohistochemistry to examine the expression profile of CCL5 mRNA in the adult rat brain and provide evidence of its cellular localization. The expression of CCL5 mRNA and protein, together with its receptors, in selected brain cell populations proposes that this chemokine could be involved in neuronal/glial communication. In this study, we have analyzed CCL5 mRNA expression patterns in the rat brain using in situ hybridization combined with immunohistochemistry to detect specific cell types (Grabinski et al., 2015; Lanfranco et al., 2017) . RNAscope in situ hybridization combined with immunohistochemistry revealed that in the CA1 region, in addition to neurons (Figures 6A1-4) , microglia (Figures 6B1-4) and oligodendrocytes (Figures 6E1-4) expressed CCL5 mRNA. Our data shows that under physiological conditions CCL5 mRNA is constitutively expressed in all glial cells (microglia, astrocytes and oligodendrocytes) as well as in a subset of neurons.	./cache/cord-002838-thygu6at.txt	./txt/cord-002838-thygu6at.txt