id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002982-zwvesrct	Thiessen, Lindsey D.	Development of a quantitative loop-mediated isothermal amplification assay for the field detection of Erysiphe necator	2018-04-20		.txt	text/plain	5660	264	45	More recently, a loop-mediated isothermal amplification (LAMP) assay was successfully designed for field use in the grape powdery mildew pathosystem; however, false negatives or false positives were prevalent in grower-conducted assays due to the difficulty in perceiving the magnesium pyrophosphate precipitate at low DNA concentrations. The detection of airborne pathogen inoculum has been improved through the development of quantitative PCR (qPCR) assays that allow for near real-time monitoring of inoculum concentration (Carisse et al., 2009b; Rogers, Atkins & West, 2009; Temple & Johnson, 2011; Thiessen et al., 2016) . The use of a fluorescence resonance energy transfer (FRET)-based probe, allows for specific detection of LAMP products and target quantification from field samples without inhibiting amplification , and several portable fluorescence-reading LAMP devices have been made commercially available, such as the Genie (Optigene Ltd., West Sussex, UK) and Bioranger (Diagenetix, Inc., Honolulu, HI, USA).	./cache/cord-002982-zwvesrct.txt	./txt/cord-002982-zwvesrct.txt