id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003092-3owcqt3d	Iketani, Sho	Viral Entry Properties Required for Fitness in Humans Are Lost through Rapid Genomic Change during Viral Isolation	2018-07-03		.txt	text/plain	8948	392	46	These results utilize a method for identifying genome-wide changes associated with brief adaptation to culture to highlight the notion that even brief exposure to immortalized cells may affect key viral properties and underscore the balance of features of the HN-F complex required for fitness by circulating viruses. Deep genomic sequencing of nine sets of paired clinical samples (primary nasal swabs in viral transport medium) and culture isolates (culture harvest from zero passage virus) led to discovery of a number of HN mutations associated with rapid evolution in culture. To assess the frequency of mutations identified earlier, we also performed deep sequencing of 118 HPIV-3 clinical samples and culture isolates from the University of Washington Virology Laboratory, allowing us to confirm that the alterations associated with brief exposure to culture for viral isolation were almost entirely found in the sequences of culture isolates and found commonly within populations of viruses in those isolates.	./cache/cord-003092-3owcqt3d.txt	./txt/cord-003092-3owcqt3d.txt