id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003623-n01rgqyv	Schuh, Amy J.	Comparative analysis of serologic cross-reactivity using convalescent sera from filovirus-experimentally infected fruit bats	2019-04-30		.txt	text/plain	6363	277	37	To evaluate the ability of our system comprising seven filovirus-specific indirect ELISAs to predict the filovirus species most antigenically similar to the species responsible for past infection, we tested seven Marburg virus convalescent serum 35 or whole blood 36 samples collected from experimentally inoculated ERBs. Five of these samples www.nature.com/scientificreports www.nature.com/scientificreports/ were collected four weeks post primary Marburg virus inoculation 35, 36 , while two of the samples were collected at 23 and 27 weeks post primary inoculation following a "natural" boost (i.e., Marburg virus-specific antibody levels waned in these bats and then increased following contact with infectious cagemates) 36 . Although significant levels of serological IgG cross-reactivity were observed between the prime-boost filovirus-specific antisera and some of the filovirus antigens, when the overall covariance of the seven-individual indirect ELISAs in the system were considered, we were able to predict the filovirus species responsible for past infection 100% of the time using as little as 25 μL of sera (each serum was tested against each antigen in duplicate).	./cache/cord-003623-n01rgqyv.txt	./txt/cord-003623-n01rgqyv.txt