id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003655-uo0hdrgc	de Vries, Rory D.	Paramyxovirus Infections in Ex Vivo Lung Slice Cultures of Different Host Species	2018-03-27		.txt	text/plain	3061	179	56	Here, we describe a protocol for the preparation and ex vivo infection of lung slices from different mammalian host species with various respiratory paramyxoviruses expressing fluorescent reporter proteins, and suggest follow-up experiments including immunohistochemistry, flow cytometry and confocal microscopy. The combination of these viable lung slices with recombinant viruses expressing fluorescent reporter proteins [7] [8] [9] allows for accurate, sensitive and reproducible assessment of respiratory virus infection and dissemination over time. We have validated this technique by infecting lung slices of multiple host species (cotton rats, ferrets, dogs and macaques) with various paramyxoviruses expressing fluorescent reporter proteins (measles virus (MV), canine distemper virus (CDV), human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV)) [10] . Using a (blunt-end) needle or flexible catheter, the fresh lungs are inflated through the trachea (or primary bronchus, if inflation of a half lung or single lobe is desired) with low-melting point agarose mixed with culture medium.	./cache/cord-003655-uo0hdrgc.txt	./txt/cord-003655-uo0hdrgc.txt