id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003697-vmmlxr0o	Zhu, Yang	Efficient Production of Human Norovirus-Specific IgY in Egg Yolks by Vaccination of Hens with a Recombinant Vesicular Stomatitis Virus Expressing VP1 Protein	2019-05-16		.txt	text/plain	7458	382	53	Here, we developed a highly efficient bioreactor to generate high titers of HuNoV-specific IgY in chicken yolks using a recombinant vesicular stomatitis virus expressing HuNoV capsid protein (rVSV-VP1) as an antigen. HuNoV is the major food-and water-borne virus that accounts for more than 95% of nonbacterial acute gastroenteritis worldwide, but this percentage may be underestimated due to In this study, we developed a highly efficient bioreactor for large-scale production of chicken egg yolk IgY antibodies using rVSV-VP1 as an antigen. Percent of blocking activity was calculated by comparing the OD values measured with or without blocking by the chicken IgYs. As shown in Figure 5 , total IgY antibodies isolated from egg yolks of rVSV-VP1 vaccinated groups were capable of blocking the binding of HuNoV VLP to HBGAs (A, B, or O 	./cache/cord-003697-vmmlxr0o.txt	./txt/cord-003697-vmmlxr0o.txt