id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003827-65s65ojc	Park, Jeong-In	Tumor-Treating Fields Induce RAW264.7 Macrophage Activation Via NK-κB/MAPK Signaling Pathways	2019-08-11		.txt	text/plain	4729	267	47	MATERIALS AND METHODS: We subjected RAW 264.7 mouse macrophages to clinically relevant levels of tumor-treating fields (0.9 V/cm, 150 kHz) and evaluated alterations in cytokine expression and release, as well as cell viability. RESULTS AND DISCUSSION: Our results indicated that tumor-treating fields treatment at 0.9 V/cm decreased cell viability and increased cytokine messenger RNA/protein levels, as well as levels of nitric oxide and reactive oxygen species, relative to controls. 16 RAW 264.7 cells were treated with TTFs or LPS for 24 hours, and the mRNA expression levels of proinflammatory cytokines such as IL-1b and TNF-a were determined ( Figure 3A) . 17 Tumor-treating fields-administered RAW 264.7 cells showed increased phosphorylation of IkB-a and p65, indicating that the p65 subunit of NF-kB was released from IkB-a, allowing for its translocation to the nucleus to regulate the transcription of many genes that activate macrophages (Figure 4 ).	./cache/cord-003827-65s65ojc.txt	./txt/cord-003827-65s65ojc.txt