id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-006971-5xgurlue	Ozer, Tugba	Review—Chemical and Biological Sensors for Viral Detection	2019-12-19		.txt	text/plain	8120	460	48	43, 44 Various nanomaterials have been utilized in nucleic acid-based biosensors due to properties such as large surface area, high conductivity, and strong affinity toward bioreceptor probes with reactive groups such as thiols leading to high sensitivity and low limits of detection. 72 Hybridization of DNA probes with long single-stranded nucleic acids requires the target first being completely denatured to remove inhibitory secondary structure, followed by time for limited numbers of denatured target genomes to interact with surface-bound probe DNAs. Hybridization in this context is similar to hybridization performed in nuclease protection assays, which use long RNA probes (200-500 bases) with a short 95°C denaturation step followed by 12-16 hr incubations at high temperatures (55-65°C) in high ionic strength solutions to reduce secondary structure formation and achieve efficient and specific hybridization.	./cache/cord-006971-5xgurlue.txt	./txt/cord-006971-5xgurlue.txt