id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-012995-1acymenq	Cameron, Lydia C.	A putative enoyl-CoA hydratase contributes to biofilm formation and the antibiotic tolerance of Achromobacter xylosoxidans	2019-08-06		.txt	text/plain	5526	303	40	We chose to further characterize a gene identified most frequently in our screen (echA) encoding a putative enoyl-CoA hydratase that, when disrupted, leads to a decrease in biofilm accumulation and increased susceptibility to multiple classes of antibiotics. We took interest in the gene encoding a putative enoyl-CoA hydratase (Axylo_0405) given the importance of homologous proteins in fatty acid signal biosynthesis and biofilm development among diverse bacterial species. Enoyl-CoA hydratases are central to the biosynthesis of a class of fatty acid signaling molecules, or diffusible signal factors (DSFs), that have been described in diverse bacterial species for their role in mediating virulence, motility and biofilm development. Since the CV staining approach used in the initial mutant screen relies on a dye that stains not only cells, but all biomass adhering to the microtiter plate, we elected to use additional biofilm assays to further characterize the biofilm phenotype of ΔechA, and whether disruption of the putative enoyl-CoA hydratase negatively impacts a specific stage of biofilm development (e.g. attachment, matrix production, maturation).	./cache/cord-012995-1acymenq.txt	./txt/cord-012995-1acymenq.txt