id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-013503-tjotro5h	Herrmann, Helena A.	Flux sampling is a powerful tool to study metabolism under changing environmental conditions	2019-09-02		.txt	text/plain	6343	337	47	By constraining a leaf metabolic model to the two conditions and using an appropriate flux sampling algorithm, we were able to explore inherent metabolic robustness to temperature and predict the metabolic changes required to support a photosynthetic acclimation response to cold. Using the experimental data shown in Fig. 3 to constrain the CO 2 input and the malate, fumarate, and starch accumulation reactions using the Arnold model (please see methods for further details), we were able to compute converged flux sampling distributions for all reactions. We did so for both control and cold conditions, which allowed us to overlay the sampling distributions of reaction fluxes and to assess changes required in plant metabolic behavior for acclimation (Fig. 4) . In order to demonstrate how the application of an objective function for an FBA analysis can lead to vastly different conclusions, we have overlaid FBA results for maximum biomass production (under the same model constraints as applied for the sampling) over the flux sampling distributions (Fig. 4) .	./cache/cord-013503-tjotro5h.txt	./txt/cord-013503-tjotro5h.txt