id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-016144-280kwlev	Maan, Sushila	Novel Molecular Diagnostics and Therapeutic Tools for Livestock Diseases	2018-04-26		.txt	text/plain	6526	364	45	Further, modifications in PCR-based molecular detection techniques have generated a vast array of fast, reliable and specific assays which have widespread applications in veterinary diagnostics. The sensitivity of any genome detection-based method can be enhanced to a very high degree by manipulating any of the three pillars of the assay, i.e. by amplification of target, signal and probe. Common real-time PCRs include (1) SYBR green method where the fluorescent dye SYBR green binds to random dsDNA and can also give nonspecific amplification and (2) dual dyelabelled probe method which involves the use of sequence-specific DNA probes that are labelled with a fluorescent reporter, permitting specific detection after hybridization of the probe with its complementary sequence. To overcome these limitations and to increase efficiency comparable to symmetric PCRs, linear after the exponential (LATE)-PCR was developed based on primer pairs purposely designed for use at unequal concentrations to yield specific single-stranded DNA products in a robust way (Pierce et al.	./cache/cord-016144-280kwlev.txt	./txt/cord-016144-280kwlev.txt