id author title date pages extension mime words sentences flesch summary cache txt cord-262274-oununr9g He, Wei Comprehensive codon usage analysis of porcine deltacoronavirus 2019-09-16 .txt text/plain 4424 225 51 In this study, we analyzed the codon usage pattern of the S gene using complete coding sequences and complete PDCoV genomes to gain a deeper understanding of their genetic relationships and evolutionary history. When the actual ENC-plot values of these sequences are lower than the standard curve, it is suggestive of natural selection playing a role in driving codon usage bias (Fuglsang, 2008) . Overall, the above results indicate that the effect of mutation pressure is in all codon positions, but natural selection plays a major role driving the codon usage bias of PDCoV. We found that the A ≠ U, C ≠ G, for both the S gene and whole genomes, which indicates the inequivalent role of mutation pressure and natural selection in shaping the codon usage of PDCoV. Briefly, PDCoV has a low codon usage bias, which was affected by natural selection, mutation pressure, and dinucleotide abundancy. ./cache/cord-262274-oununr9g.txt ./txt/cord-262274-oununr9g.txt