id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-270911-z637eh2z	Zhou, Jie	Differentiated human airway organoids to assess infectivity of emerging influenza virus	2018-06-26		.txt	text/plain	5003	274	47	title: Differentiated human airway organoids to assess infectivity of emerging influenza virus airway organoid | proximal differentiation | influenza virus | infectivity I nfluenza A viruses (IAVs) can infect a diversity of avian and mammalian species, including humans, and have the remarkable capacity to evolve and adapt to new hosts (1) . Current in vitro models for studying influenza infection in the human respiratory tract involve shortterm cultures of human lung explants and primary airway epithelial cells. We chose to compare the infectivity of H7N2 with that of H7N9/Ah in the PD organoids as a proof of concept, to verify that the differentiated AOs can indeed simulate human airway epithelium in the context of influenza virus infection. Fig. 4 shows that viral loads in the cell lysate and medium of H7N9/ Ah-infected organoids increased gradually after inoculation; the viral titer increased more than 3 log 10 units within 24 h, indicating a robust viral replication.	./cache/cord-270911-z637eh2z.txt	./txt/cord-270911-z637eh2z.txt