id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-276587-ynionj5r	Hwang, Mihyun	Alpha/Beta Interferon (IFN-α/β) Signaling in Astrocytes Mediates Protection against Viral Encephalomyelitis and Regulates IFN-γ-Dependent Responses	2018-04-27		.txt	text/plain	9050	552	49	Uncontrolled viral spread throughout the CNS parenchyma of mGFAPcre IFNAR fl/fl mice not only was associated with increased astrocyte infection but also affected neurons and microglia, despite overall elevated and sustained levels of mRNAs for IFN-␤ and IFN-␣ genes and ISGs. IFN-␥, a crucial mediator of MHV control in the CNS, was not impaired, despite reduced T cell CNS infiltration. To evaluate the kinetics of MHV A59 replication relative to Ifn␣/␤ and Ifn␥ mRNA levels in the CNS, brains from uninfected and infected wild-type (wt) C57BL/6 mice were harvested out to day 21 p.i. Virus replication was monitored by expression of viral RNA encoding the N protein (A59 N), which is present on genomic and all subgenomic RNAs (33) . Under the assumption that viral mRNA levels reflect similar replication in both glial populations, these data support microglia as superior initiators of IFN-␤ production relative to astrocytes following MHV A59 infection in vivo; these findings are consistent with results from primary neonatal cell cultures (18, 29) .	./cache/cord-276587-ynionj5r.txt	./txt/cord-276587-ynionj5r.txt