id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-277187-rcxjjxw3	Xu, Zhichao	Attenuation and characterization of porcine enteric alphacoronavirus strain GDS04 via serial cell passage	2019-11-04		.txt	text/plain	5894	302	54	Porcine enteric alphacoronavirus (PEAV) was first detected by our team via genomic analysis of samples collected from a diarrhea-outbreak swine herds routinely vaccinated with porcine epidemic diarrhea virus (PEDV) vaccine in a farm in Guangdong, China in February 2017 (Gong et al., 2017) . The clinical symptoms caused by PEAV in newborn piglets are similar to that by other porcine enteric pathogens such as PEDV and transmissible gastroenteritis virus (TGEV), which include vomiting, diarrhea, dehydration, and mortality rate as high as 90% (Gong et al., 2017; Zhou et al., 2018b) . To develop a live-attenuated vaccine for PEAV, we generated candidates via serial cell passage of the parental PEAV GDS04 strain and evaluated the pathogenicity of PEAV P15, P67 and P100 in 5-day-old newborn piglets. To generate an attenuated PEAV vaccine candidate, PEAV GDS04 was passaged regularly to a total of 100 passages in Vero cells and the selected passages including P15, P67 and P100 were characterized by sequencing and analyzing the complete genome (Fig. 1) .	./cache/cord-277187-rcxjjxw3.txt	./txt/cord-277187-rcxjjxw3.txt