id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-279503-w4tn03w0	Kim, Hanbi	Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles	2019-05-07		.txt	text/plain	3541	185	49	In this study, we propose a colorimetric assay based on an extended form of double-stranded DNA (dsDNA) self-assembly shielded gold nanoparticles (AuNPs) under positive electrolyte (e.g., 0.1 M MgCl(2)) for detection of Middle East respiratory syndrome coronavirus (MERS-CoV). This assay could be highly reliable for MERS-CoV diagnosis as we have followed WHO updated recommendations for infectious disease laboratory testing, which targets the two regions on MERS-CoV considered for potential preclinical screening and high sensitivity 20 The developed assay platform was able to detect the target DNA through optical properties of the gold nanoparticles such as color changes with the naked eye and spectral shifts on UV− vis wavelength. We proposed a colorimetric assay using disulfide bonds formed by hybridizing with thiolated probes and a target; this method inhibited the aggregation of AuNPs by salt and limits the color change for diagnosis of MERS.	./cache/cord-279503-w4tn03w0.txt	./txt/cord-279503-w4tn03w0.txt