id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-282062-h9smg0w9	Takano, Tomomi	Novel single-stranded, circular DNA virus identified in cats in Japan	2018-09-14		.txt	text/plain	1942	124	56	We detected a novel feline stool-associated circular DNA virus (FeSCV) in fecal samples from cats with diarrhea using consensus primers matching those of circovirus and cyclovirus. Feline cyclovirus was identified by next-generation sequencing analysis in which the viral gene was detected in a pooled fecal sample collected from 4-5 healthy cats. In this study, we performed nested PCR using Circoviridae family consensus primers and detected novel CRESS DNA viruses in several cats with diarrhea symptoms. However, we concluded that FeSCV is a circular DNA virus based on the following: 1) No Giardia intestinalis was detected in the fecal test, and 2) the complete genome of FeSCV was amplified using the rolling-circle amplification and inverse PCR assays. We detected a novel CRESS DNA virus, FeSCV, in fecal samples from cats. Although it was detected using consensus primers of circovirus and cyclovirus, FeSCV was phylogenetically positioned in a clade different from that of these viruses.	./cache/cord-282062-h9smg0w9.txt	./txt/cord-282062-h9smg0w9.txt