id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-283881-yhed5s98	Karelehto, Eveliina	Polarized Entry of Human Parechoviruses in the Airway Epithelium	2018-08-22		.txt	text/plain	5889	313	47	To bridge this gap, we determined the polarity of infection, replication kinetics, and cell tropism of HPeV1 and HPeV3 in the well-differentiated human airway epithelial (HAE) model. Given that integrins and heparan sulfate have been reported to be expressed at the basolateral surface of polarized epithelium (Erlinger, 1995; Esclatine et al., 2001; Lütschg et al., 2011) , and that HPeV1 has been detected in respiratory patient samples more often than HPeV3 (Harvala et al., 2008) , we hypothesized that HPeV replication efficiency would differ depending on the inoculation site and the HPeV genotype. Furthermore, we speculated that differences in the airway epithelium host response may contribute to the distinct clinical outcomes and performed transcriptome analyses to compare the HAE gene expression profiles induced by HPeV1 and HPeV3 infection. To investigate HPeV replication in the human airway epithelium, we inoculated well-differentiated nasal HAE cultures, established from four individual donors, with HPeV1 and HPeV3 either from the apical or the basolateral surface (Figure 1 ).	./cache/cord-283881-yhed5s98.txt	./txt/cord-283881-yhed5s98.txt