id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-284549-edliu3it	Zhou, Hui	Hepatitis C Virus NS2 Protein Suppresses RNA Interference in Cells	2019-11-27		.txt	text/plain	4697	289	62	In this study, we screened all the nonstructural proteins of HCV and found that HCV NS2 could suppress RNAi induced either by small hairpin RNAs (shRNAs) or small interfering RNAs (siRNAs) in mammalian cells. In this study, we uncovered that HCV nonstructural NS2 protein possessed a potent in vitro VSR activity that suppressed the RNAi induced by short hairpin RNA (shRNA) and siRNA in mammalian cells. Our results showed that the reversal effect of EGFP silencing could be observed at 48 hpt (Fig. 2C) , indicating that the VSR activity was dependent on the expression level of HCV NS2 protein. To investigate whether HCV NS2 can inhibit this step, small RNAs harvested from HEK293T cells co-expressing EGFP-specific shRNA together with NS2 were subjected to Northern blotting with a DIG-labeled RNA probe targeting EGFP siRNA produced from shRNA by Dicer.	./cache/cord-284549-edliu3it.txt	./txt/cord-284549-edliu3it.txt