id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-291754-1zxztadu	Zhao, Ye	Successful establishment of a reverse genetic system for QX-type infectious bronchitis virus and technical improvement of the rescue procedure	2019-10-15		.txt	text/plain	6848	344	54	In this study, a pathogenic avian infectious bronchitis virus (IBV) QX-type strain YN was successfully rescued by vaccinia virus based reverse genetic technology. To compare the in vitro replication of the rescued virus rYN and its parental strain YN on CEK cells, 200 μl PBS containing 10 3.0 TCID 50 of rYN or YN virus were inoculated onto the CEK cells in 24-well plates, and 200 μl supernatants from three wells from each group were harvested at the time points of 6, 12, 24, 36, 48, and 60 hpi for a real-time PCR detection assay for IBV N gene as described above. Collectively, these results demonstrate the successful rescue of the pathogenic IBV strain YN from cloned cDNA by using electroporation of full-length IBV in vitro transcripts into N-protein expressing cells and subsequent virus amplification in the allantoic cavities of ECE.	./cache/cord-291754-1zxztadu.txt	./txt/cord-291754-1zxztadu.txt