id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-295661-v3q1spmm	Resende, Talita Pilar	Evaluation of mouse enteroids as a model for Lawsonia intracellularis infection	2019-07-19		.txt	text/plain	5460	252	31	intracellularis antigen is detectable in mouse enteroids, indicating susceptibility to infection, mouse enteroids fail to replicate the cellular proliferation and gene expression changes observed in proliferative enteropathy. Wnt3a protein, which regulates the signaling pathways related to the cellular proliferation in the intestinal crypt compartment [33] , was removed from the enteroid culture media at least 3 days before infection to enable cells to better differentiate. intracellularis infection induces changes in the proliferation and differentiation of enteroid epithelial cells, as observed in the swine intestine, we evaluated expression of Ki-67, Sox9 and Muc2 in enteroids harvested at 1, 3 and 7 dpi relative to expression in enteroids at 0 dpi. intracellularis antigen stained by immunohistochemistry is observed as small bacilli in the cytoplasm of cell cultures [5, 47, 48] and in the apical cytoplasm of intestinal epithelial cells of naturally and experimentally-infected pigs.	./cache/cord-295661-v3q1spmm.txt	./txt/cord-295661-v3q1spmm.txt